LOCUS       DAIAHG010000001        32245 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_1_length_32245_cov_3.264828, whole genome shotgun sequence.
ACCESSION   DAIAHG010000001 DAIAHG010000000
VERSION     DAIAHG010000001.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 32245)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32245)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..32245
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1_length_32245_cov_3.264828"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    12763..12772
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9759 a         7074 c         6244 g         9158 t
ORIGIN      
        1 aaaggttttt ttctccgtgt tgataatccc catcaaaagc gtcgcaaact gccccacatt
       61 caccatttca tggaaccgtc ttgcataaaa aaactctctt tttttcatga aacgcgctat
      121 agtcaaggaa aataaatcac acattacctc aaggagaagc ctgtgcttga aaacctccac
      181 ctcacgacag aaaaggatgc cctcatgaca gagacggatc ttgtgaattc aaactcaaat
      241 gataataaaa aagtccttgt gaccttggat attaacgaga ttatggcgct catcccccat
      301 cgctatccca tgcttttgat tgaccgcctt gaaattcttg ccttttcaga aaaagccatt
      361 ggcattaaaa atgtatcgat gaacgagtgg tattttgaag gtcattttcc aggaaaaccg
      421 attatgccag gtgttcttat tgttgaggcc atggcccaag cagcagcagc ccttgtgatg
      481 aaatccatgg aggttgatgg cgtagatctt gccagtaagc ttgtttattt tatggccatc
      541 gaggaagcta aattccgtcg ccctgtggtg ccaggagatg cgcttcgcct tcacgtgaca
      601 aaagaaaaaa gccgcggccc tgtgtggcgc tttaagggcg aaggtattgt ggatggaaaa
      661 attgctagtg aagctaattt tacagcgatg attgtggatc ggtaggtttt tatgtccatt
      721 gcaacgatca cagatcttaa accatcgatt cacccaacag cgattatttc gaatacagcc
      781 gttattggcg atcatgtcag cattggcccc tactcttatg taggagacca cgttcatgtc
      841 ggcgcccata ccactcttaa agcccatgtt gtcatagaat cctatacgac aattggtgag
      901 cactgtacgg ttcaccccta tgccgtgatt ggcaatgatc ctcagtccgt tagttataag
      961 ggcgagccta caaaaacaat gattggtcat cacacagtca tccgcgaaca tgcaacgatt
     1021 aatcgcggta cagtccaagg acgtggtgag acgcgcgttg ggaatcattg ttatctcatg
     1081 acgtcctgtc atattgccca cgatagtatc gttggcgatt atgttcagtt tgctaattgc
     1141 gcaaccattg gcggtcacgt tgttgtgggc gattatgcca ttcttggagg actttgtgca
     1201 gtgcatcagt ttgtccgtat cggccatcac gccatgatca gcggcatggc cggtgtgaaa
     1261 tatgatgtca ttccttatgg ccttctcagt gcagaggata caaagctaag cggtttgaat
     1321 cttattggtc ttaaacgtcg tggtttttca agtgatgaaa tccaaagcat ccgccgtgcg
     1381 tataatgaac ttttcgacca caaggaaaaa ccttttatcg aacgccttgc ggatgtaaag
     1441 aaattttaca aagattcaaa ggctgttgag caacttttgc actttatcga ggaacaatcc
     1501 gatcgctcgc tttgttttcc ttcggactaa gcaagagatc atctctattt tatcttaagc
     1561 attttaaaaa tattaatcat ttcatattac taaaaaacta aaagaccatt ttttttaatg
     1621 agttgtttac tttttttgtg taagaatgcc caaagaaaat cattcaagat tggctttgga
     1681 aaatatgaaa ttattcatca attttttgtc ttttttgatt atttcattag cacaatgtgc
     1741 gcatgcaagc aataatgagt actcagcgta tataaaagat atcaaaaatc agctcaaccc
     1801 agatacgtat acttactgtg caaaaaagga agtagccttt aatccagaaa tcttcacaga
     1861 gggcaatggc cctgaggctt tatttatgac agggatgtat tatttcatga tgggagaaaa
     1921 gcctcccaaa tatctaaagg tctttgaaaa agcagcagag gggggaagca atagcacagg
     1981 agacgataat aagacatgtg acaattatgc ctgtttagtg ctgggtattt tttataaaga
     2041 gggagccttt ggcatcgaga aaaacccaga aaaatcagaa aattatttca tcaaaattac
     2101 gcatattaaa aattctacat ttgaaagaga aaatttttta aaacttattc atgattttca
     2161 taaacacctc tttcattcta atgagtataa aaaaaatatt aatcaaagtt ttctatctga
     2221 atttgatact ctttttaccc tcaaggaaaa aaatgaagaa gagataataa aaatcaatgt
     2281 cattgaagaa aaaaagaatg aaatcaaaaa agattttgat ttaggtaaga aaaaaaaatc
     2341 tcttataaac gctattttcg gtaaaaaaaa taaaaaagaa actaataata aagaggataa
     2401 aaaactccaa tcatttttga atgacactca taatcaaagt aaaagacgac aatctatcat
     2461 cggcgctttt ctaaagaata ataaaaagaa ggaagataag gaaaacaata ataaaaatct
     2521 aatcagtcaa acgcctctga cgaaatttaa ttcttttgga aaaatagacg acaaaaaatg
     2581 agattaaaaa tgagtccaat ttgaaaaaag aagattcatt ttaagattta agagagggcg
     2641 tgtgctcctc tcttaaaaag gcattttgaa cccaggaggc aagggcatac ccgctgtggc
     2701 tttgctcatt tcctctgcac ttttcgcttc gacctttgcg cgtgcatcat taagagccgc
     2761 tgtgatgagg tcctcgagca tctccgcgtc attcggatca ataatcttag gatcaatttt
     2821 aatcttaagc acgtcgaact ttccattcat tgtcacctcg accatgcctg cacctgagtt
     2881 gccctggacc tcaatcaacg caattttttc ttgcgcctca ttcatctttt gctgcatttt
     2941 ttgagcctgc tgcatcagcc ctttcatatt tccaaacatt tactatcctt ttgagtattt
     3001 tatctgatgt tctcccacaa tagcattgac gctcaaaaag cgcgataaga aaggatcatc
     3061 actgtaaaat agtttatttt tccctttaaa tatgaagctg ttttgattgc gtcttaacga
     3121 tgaaattgct aaaatgacga caaaaatacc ccccattctc acgcatacac atcaaggaaa
     3181 atcaatggcg aaatcgcaac aaaacgtggc tcttctcatg ggaggatggt cagcagaacg
     3241 agaggtttcc ttaagttcgg gcggagaaat tgccaaagct ttggagaaaa tggggcacgc
     3301 agtccgtgtg atcgatgtaa ccgacgactc tgaagcgctt ttgcagcatt taacgccccg
     3361 tcctgatgtt atttttaatg cccttcatgg cacgggcggt gaggatggtg taatccaagg
     3421 atttcttgat gttttaaaaa ttccctatac gcactcgggc gttgcggctt cagcgattgc
     3481 tatggataag gttttatcgc gcaaaatctt tgagcatcat ggcatcccaa cacctccttg
     3541 ggctcttgtt aaacgcatga gccttaagga tcatctacct tttgagaaac cttttgtgat
     3601 taaacccctc aaaaatggct ccagccgtgg cgtttacatt gtaaaagaca cattgcctga
     3661 agaaattttt tcagacgctt gggcttttgg tgacgatctt ctcattgaaa aatatctgcc
     3721 aggacgagag attcaggtag gtgttttagg tgaaaaagct cttggtgcca ttgaaatttg
     3781 ccccaaaaca gaattttacg actatcaagc caaatacacg gagggttttg cagagcatat
     3841 tatgcctgca ggtctttcaa aagatgatta tgatcacgtt ctggagcttg gcctcagagc
     3901 acataatgcg ttaggttgcc gcggggttag ccgtgtcgat tttatttttt atgagggtac
     3961 cttttacctt ttagaggtga atacacatcc tgggatgacc cctttgtcgc ttgttcctga
     4021 aattgcggcc tataagggcc tctcttttga agatgttctt cagcatttgc tcaatcatgc
     4081 tacctatagg acataaatgt aatgcgggtt tcacgcctta aactatcgcg caagaaaaac
     4141 ccaaagagct attttttagc cccctttctt ctttttttaa gccggtttcg tcgtcgtttt
     4201 tttctcatca gccctctttt tatcatagga gctttgagcg taacgtggca attcggttat
     4261 attcaatctt tttatgattc tttggcgaca aaaacctctg atcttttaag ccggtgcggc
     4321 tatcgctttc atgatattta tattcaaggg cgcacattta caaccatgca ggaaattttg
     4381 caggctgtga atatgtatca aggagatcct agttttaaat acagcccatc agatattaaa
     4441 aatcgtttgg agcaactccc ctggattgct aaagcggatg tgcgacgtga acttccaggt
     4501 gccctgcata ttcatatgag tgagcgtaaa cccattgctc tatggcagca tcagcagcgc
     4561 ttttacctcg tagatgaaga gggtattgtt attcaagcct ctatgctccc ccaatttcaa
     4621 catcttccca ttgttgtggg gcaagacgct cccttgcaga cacctaaaat cttagccatg
     4681 ctcggtcatt ataaggaaat tttaacacgc atcacaggca ttgtacggat gaataaacgt
     4741 cgatgggatc ttatccttca taaaaaattg acaattaaac tcccagaaac acaggtagaa
     4801 gcagcgctgg cacgtttaaa ttttttgtta gaggcgcata aaattaatct cgacgaagtg
     4861 acagttattg atctccgttt gcaaaaacaa cttattgcca aagtatcccc ccatgcagcc
     4921 gctcgtctca agcttaaggg tgtgagtgct taaacattaa tttagagtag caagattcta
     4981 ctttttatcg ctcgcatctt tcggcgtgaa cacaaactta gcaatcaagg actctaaact
     5041 aatggagggc tgtgtataaa cgattgtctc cccatttttt aaatagtgct cgctttctcc
     5101 aggaacaatt gccacatact tagaccccat aagcccatca ctcgcaatct ccgcaacgga
     5161 atccaccgga atgttataat ccttatcaaa gccaaaggta agctccgcca aaagattttc
     5221 cccagcaaaa cgaatgcctg tcacctcacc aaccttcacc ccacgaagct tgacatcgct
     5281 gcccattaaa atgccatcta cacgctcaaa cttagcattc agatgaaatg tttgataact
     5341 cgctatggtc gtattggcct tgtaggcata gttgaaaaaa atccccgcaa ccaaaagcac
     5401 aaaagcaccc ataatcattt caagataatt tgtacgcatc aaaatgcaga tatccttatt
     5461 tttccatata gcttttggcc atccaccggt atttttcaag gtgattaacg agatcaatga
     5521 gaatatcttg tgtcacaaga tcaccagatt tttcacacat gccccagagg cttttccctt
     5581 gagagatgac agtctcgcaa ttttttataa aaatctcaac gacttttagc tctcttaatg
     5641 aaatctccga ggaatcttcg agctcttgca agcggtggat atcagaaaga gtgcctgggg
     5701 catagtcatc gcaggcacga atgcgctccg caagcgtatc aatatgttca aaaagatctt
     5761 tataaaccat ttcaaaaaaa ccatggagtt ggaaaaagtg atgccctttc acattccaat
     5821 gaaagccttg aacctgggta aaaagagcat agacattgct cattaaaatt ttcatagggt
     5881 gcatataatg ctctgtcatt tttatcgact ctctaaacat atctttatgc tttaagtcta
     5941 cgcctcatat cttaaaaaaa gattaatatc ttaaaaaaaa ataccccgac caaaggggga
     6001 gcaaggtcgg ggtactgagc tcccttctga caaaggagca ccgccgcgga ggagcccttt
     6061 cagactcaac tcaacgccta cgggtacata tacagctctg cgcgccgtat ttcttctgac
     6121 gtaataggag ggggaacaat atcttgtcct ctcttgtcct cttcaacaag agctaattct
     6181 tttttattat caggctctcc tggttcatgg gtcagctctt ttttaagcgc tggatccact
     6241 acattttcaa caattgctct ctcttccaat tctttttcta gagccttttg agccatggcg
     6301 gcatggggat caatgggcgt tacagcatca tccggtgtgg gactgtgtgg cgctggcaca
     6361 ccatcctcaa tatccccctc tcttagcctt aggccttcct ccgatgcctc ttcaatccaa
     6421 ggaagtgcga ttgtttcctt aaggctcgtt ccatgacttt taaagtaatc ctttaaaacc
     6481 gtgttatctt ttttctccgt atcataaggc gatggggcca cctgagaccc ctcccctgtc
     6541 ttttcagggg cataaggcgc actattttca gaaggttctg ccgcaaagct cagccctgcg
     6601 caaaaattta caaagattaa gaaaaaccag cgcatcatat ctctgcccat cacattattt
     6661 aaaatttccc tctaaggatc agactaacgc ttaaatatta ataaaaagtt aatatatttc
     6721 ccctaaaaca ctctgtttaa aaaaataata ttatgaggat aaccccttgt tttttgggca
     6781 taaggcccct atcttaaagg aaaaatcctt tcttttagat cattatgttg acccttacac
     6841 aggctgcggc ggcacgtatt tccgccatta atgcagaaaa taaaaatcaa aaacgcctgc
     6901 gtattgccgt tcaagggggc gggtgctccg gctttcaata cacatttctt atggatgatg
     6961 ccgttttaat ggatgatcac attttttcac atcaggatgc cgaaattatt attgacgaaa
     7021 tgtccctttc tcttctgggt ggaagcacac tcgattatca agatgatctt gccgcatcga
     7081 tgttcaccat cacaaatcca aacgctaaaa cggggtgtgg atgcggtaat tcttttagta
     7141 tttaatattc ttttcattca tcattggcct cctcttaggc cttttcacgc gcatgaaaat
     7201 accgtacaaa gaaagaagtt tcagaaaaaa agagccatcg tccgtgacaa cattattcca
     7261 aaaacaggca aaaacactga ccattgcttc gtggaatgtg aactctgtcc gcgcaagact
     7321 cccggtttta gagagctggc tacgcctaca caatcctgat gttgtgtgtc tacaagaaac
     7381 aaaatgcgag gataaaaatt ttcccacaga agtgttctcg gaccttggat acaatagcgc
     7441 gcattttggc caaaaaacgt ttaatggtgt ggctatttta tctaaatttc cttttgatga
     7501 aattgtgcgc gatattccaa atcttgagca tgatcaggcg cgttatatcg aggcaggcat
     7561 cagcgccttt ggtcagtatt ttcgcgttgc atccatctat gtccccaacg gtcaagaggt
     7621 tggcagtgac aagtatgatt ataaattaaa ataccttgag gccttaaggt tgcacatgca
     7681 gcatcttttg agctatgacg agatggctct tttttgtggc gattataaca tcgcgcctac
     7741 ggataatgat gtctatgatc cagcgtcttg gcatgagaaa attttgtgca gtacacctga
     7801 acgcaaggcc tttgcagcac tcacccacct tggctacacc gatattttag gatttgtcgc
     7861 tccccccccc tatacatggt gggattaccg tgcaggaagt tttcaaaaaa atcagggtct
     7921 tcgcattgac cacattttag cctcaccaaa agcaagcgac cgtgtccttt tcaatgaaac
     7981 aaaagtcgac aaagacgtac gaggtcttga aaaatcttct gatcatgcgc ctgtgatcgt
     8041 cgccctcaca ctttaaacga ttcgataaca tctttaagat gtgtattttt agctaccatc
     8101 ctcaaatcag ttatgctata gatgggacat caaattatgt taatcattgt taaaaataat
     8161 gaaaagaact tttcagccta gtaatcttgt acgcaaacgt cgtcatggtt ttcgcgcacg
     8221 tatggccact gtgggtggac gcaaaatcat cgctaaccgt cgtagagcag gtcgcaaaaa
     8281 actttcggcc tagtctttag agataaaccc atgaaagagg tgcgccttaa aaaacgttct
     8341 gaattcatgg ctttgtcaaa aagtcgccaa tctgttttta tgccgcattt tattgtccaa
     8401 tatcttttta aagaggcgca gggatttccg cgctttggat atacggtttc taaaaaagta
     8461 gggaatgccg ttgcacgtaa tcgtgtaaaa cgaaggcttc gtgagcttgt gcgccttaca
     8521 ctcaaaaaaa atcacgattt ttttactctc tcttatgata ttgtttttat tgcacgttca
     8581 tcggcgcgac aagcaccgtt tgatgagctt atcaagcatt ttgaggatgc tttgatgcgg
     8641 attacctcct ttcatactta aattttttta tgtaaaaagg cctgtttcca tgattcaacg
     8701 tttgtgtatt tttctaatac gtatttaccg ctggggtatc agccccttta aaagacaaag
     8761 ctgtcgtttc gagccgagtt gctctttgta cgccattcat gctatagaat atcatggtgt
     8821 catcaagggc ataacacttg cggttaaacg cctgttgcgt tgccacccct tttcaaaaac
     8881 ccacgggtat gatcctatcc caccctctaa tcgaatgaag catccatgaa cgaacaaaaa
     8941 aatcttatcc ttgccgttgt ccttgttatc ggtattcttt ttggctttca ctatttttat
     9001 gagcaaccca aagcacagca aacccttgca gcacgccaag agaacaaaga gttgaatgca
     9061 aaagatacac agtcccccca agaggccctc ggtttagccc atacactgac agaacttaac
     9121 cgtgatgagg cgattgcaaa gggaaaacgc attgtcatta aaacaaacaa acttgagggg
     9181 tcgattaatc ttgtaggtgg cgtcatcgat gacctcaagc tcagggatta taaagaaacc
     9241 acagacaaaa atagccctga tgtaatattg ctgagcccct ttcagacaaa aaccggttac
     9301 cttggcttta cgtcctggaa atccctggat aaaaacactc ctcttatcct tcctcaaacg
     9361 gacaccgtat ggcatacaga gagcacagag cttggtgttg gaaaacctgt aaaactcatt
     9421 tacaccaatg gaaaaggaca aacatttgag cgcactatta gtgttgacga tcattatatg
     9481 ttcaccattg aggataaaat tattaataac accgcaaatc ctcttacact caaacacatt
     9541 gcaaccgttg aacgccacga tatccccgtt tctgcacaaa gtggttttat tcttcacgag
     9601 ggtgccattg gtgtggtgaa tggtaaactc cacgaggttg actattccga catgacgctt
     9661 gagcccaagc cacaagatac ttattttaca aaagcaaaac gcagttggat tggttttacg
     9721 gataaatact ggctcacagc ccttattcca acgcctgata aagaatccac aatggttttt
     9781 ctcggcaatc acgataaaaa aatctatcaa gtccttgcag aaagccaagc ggttactctt
     9841 gaaggcggta aagaagccac tgaatcggtt catttttttg ccggagccaa ggttcttgaa
     9901 cttttggata tgtacgaaca gaagcttggt tttgataagt ttgatcttgc tgttgacttt
     9961 ggatggtttt attttctcac aaaaccgatg ctatctttcc ttcagttttt gcacaacctc
    10021 cttggaaact ttggtctcgc cattcttgcc cttacagtga ttgttaaaat tatatttttc
    10081 cctcttgcca ataaatctta cagatccatg agccgcatgc gcgcctttgc acctcagatt
    10141 gaagcgctca aagcacgcta tggcaatgat aaaatgaaac tgaatcaagc catgatggag
    10201 ctttataaaa aagaacagat caaccctatg tcgggctgtt tgcccatgat tattcaggcc
    10261 cctgtgtttt tctgtctata taaagtcctc tatgtatcgt tagatatgcg ccaggcccct
    10321 ttttacggat ggatccacga tctttccatt cctgatccta cgtcgctttt taatcttttt
    10381 ggcctgctcc cctttacacc accgcacttt ttgcaaattg gtgcatggcc aattattatg
    10441 ggcgcgagta tgtttctgca gcaaaaactt aatcctcagc cggcggacaa ggcacaggca
    10501 acgatgttta tgattatgcc gctttttatg acctttctct ttgcaggttt cccagcaggg
    10561 cttgttatct actgggcgtg gagcaatatc cttggaatcc ttcaacaatg gatgattatg
    10621 cgtatggaag caaagcgtgt gcgcatacag aaaaaataga gagacaatag agaggcatta
    10681 accctctctt aatcttttat taacctttat cacgctatcc tatcctcgtt acccacaatg
    10741 acgaggattt tttatgcgcc ttataacact tgcaaccgtc atgctgtctt tcacatccat
    10801 ttcatccatc aatgcggggg attggcgtat acatgatttt tatcctgagt ctaatggtca
    10861 gttcaatccc aatgacagag gctttctacg tattcttccg tctgaaaata tgtcgagtgc
    10921 tagcctcata ggatataaaa aaaatgaaga ggaagaaaag gattatgatt attcaaagtt
    10981 tttgtcctat attcaagtta gcgatgaatc cttttcacac actcgatggg gctctgtagg
    11041 cctgctttcc aataatcatg atactcaaat catctaccga gtgcctttaa tagaattttc
    11101 ttttgaggaa aaggatgaca aaaaatcacc tgccgaagaa gcctgggaat tctttttaaa
    11161 agaaacaaat cctaaggaaa atcttggaga gacggagaaa aatgcgaagg ataacttaga
    11221 gggagcctta aaagatctcg acctaacctc caacgacctc gatttagtct taaaagaatt
    11281 ttacgatgaa attgatgagg aagaggatga aaaggatgaa gaaaatctca aaaagtccgt
    11341 tgtgctttct tttcaaaata aagagtgaag agatcgcctt tttagtgttt actcctctca
    11401 gaacatatct ataattttta atcacttatg acctatcgcg ttcttgcgct tacgcttttt
    11461 cccgaaatgt ttccagggcc tcttgactat tctttggcag gaaaagcgct taaagaaaaa
    11521 aagtgggaga tagagacgat aaatctgcgt gattttgggc tttcaaaaca taaaaatgtg
    11581 gatgataccc cctatggcgg tggcccaggc atggtgatgc gcccggatgt tatcgatgca
    11641 gcgctccaaa aagcccatgc gcatgctccc tcgaataaga taatttattt tagtcctcgt
    11701 ggcgttcccc tcacacaaaa cctggcccaa gattttgcag cgctttctgg ggtgacactt
    11761 ttgtgtggcc gttttgaagc ggtggatcag cgcgtcattg acaaatggga tttgatggaa
    11821 gtaagtcttg gggattatat tctttccggc ggtgaagttg cagcgctcac tctcttagat
    11881 tgtattgtgc gcattcttcc cggtgtcatg ggcaaccgag aatcggatga ggaggaaagt
    11941 ttttcaaaag atcttcttga atatccccat tacacgcgcc ctcatttgtg ggaaaataga
    12001 gctgtgccag agattttact ctcaggcgat catgaaaaaa tcaagacctg gcgccgcgaa
    12061 caggccgaaa ttatcacaca acagcgccgc cctgatttgt tcgaaaaata tcaatcacgc
    12121 aaggataaaa actaatcctt atcctcttgc tcaagcgtga tatcgacaat gccttcccct
    12181 tcataaaaat ttattatctt ggcctcgtaa acaatttttt gatgcatttc cttccaccgc
    12241 gcatattcgt ttctattttt aatctcctca ccaagatttt tatagtcgta ataatcattc
    12301 tcaaagggcg agaaaaaagg tacaaagaaa ttttttcgct cttttgtatc cacaagatac
    12361 tccttgtcat cttttttaga acggacactt tgaaccttga gagggccaat atcccggtga
    12421 ccataaattg caaaagaata actcccagga actccctttc catcaatata ttcttgatac
    12481 acctcaaaat catccgtcaa aacctcaagg gtattattcc ccgtatcgct cactcccata
    12541 atgtgatttt gatacccaat gggcatttta aatttaaaat gaatcgtttt gctttttgga
    12601 ataataaaaa gccgaggaac accaagtctt ttgctatcct ttaatttctc ctgcaaatac
    12661 aaattcggga acacataacc aaaaggaact tttcggactt taaaggcttc ccactccgtc
    12721 gtgatcagat taaaaagctt accattttca gatattttgt atnnnnnnnn nncaaaaaag
    12781 ggcgccattt tctccatcat gcgctgagca tacgccctaa tctcaacaat attatacccc
    12841 cagtacggct catgaatgta attgagcgcc tttgagtatg tttccttgtg tttttctcta
    12901 aagtcgtcca ctttttcttt taaagttgtt ttctcctcac tcaggagtgt gtaaaggttt
    12961 tcaaccatgg gaaaattacc cgcttgattc gcaatacggt aggctacttc gataaaaata
    13021 aaagaacatt cttcgggctc taaattcagc tccttccctt gataaatttc taaaaatgcc
    13081 tgagtcatat cagggagttc gatctttttt tcatccattt ttttacagat ttcctcggcc
    13141 atccatccca aatcattgta aaaaagaaga tcaagacatg tgagaaatgt ctcatcatga
    13201 taaggttcat tgaaaaactc aaagttattg gaattttgaa ttaaaatatc aggaatatcc
    13261 ttattattta ttttcctctc gtcatttttg gaaaatttct ttttaaattt tgaaaaattc
    13321 cagagtgttt tgcgtgctcc ttcaggtacg taattcgcaa tagcaaaaag actttctatg
    13381 tctttgcgaa actctactaa agctttgttt tccgattttt ccgacttatc ctctgaaata
    13441 ctaatttcca atgcgtgtga agatggaagc accacaaggc aaaaaagaaa ggtaaatact
    13501 atttttctaa acatttttga aacatatttt aaaatcatat tattcttgaa cattatatga
    13561 ttaattatat taataaaaag taaaattcag gatattttta cgcacggtgg ttctctattc
    13621 gcgagatatc tcttgctaaa atctttttag gttattagaa tgagtcctgt gtaaatttga
    13681 aaaaatttat cactcttcga cgcataagat cgtgccttag tcctgctttt tggcgatcat
    13741 cccataaaaa aatcacctga acggtgcgaa gaatcagcag taagaacaac caaggagtgc
    13801 ccctccattg atgagggcac tgtagaatga aaaggtgatt ttttatgaac attattcaac
    13861 agcttgaaag cgaacaagtc gcacgcctta cggaaaataa acaagttccg atgtttcgcg
    13921 caggtgacac tgtgcgtgtg catgtcaaag tcaaagaagg tgaacgcgag cgcgtacagc
    13981 cctatgaagg cgttgtctta gggcgcaaaa tgcgcggact caactcctcc tttcgcgtac
    14041 gtaaaatgtc ttacggcgag ggtgtagagc gcgtatttcc tttgttttcc cccaatatcc
    14101 gcgtagaact tgtccgcaaa ggtaaggtct gccgcgcaaa attgtactat cttcgtggtc
    14161 gtacgggtaa aagtgcacgt attgctgaga gcaatcgcac ctacaaagac taagttttta
    14221 ttcctttttt tttagagcac gaagcccgag gattttcctt gggttttttg ctttttaggg
    14281 aatttttgac gataattgcg taatatttga gctatttttc acgaaaaaca tagaataatt
    14341 tttttggttt atttttcacg aaaagaacgg gatttttgcc tcattccagc ataaaatcac
    14401 tatttttccg gaatatcttc acgaaatttc agatttttga gactgaatcc agcaaaagca
    14461 ttgaagattt ttctaaaatt cctttcagcc cccccctcac gcgggagaaa aattttaaaa
    14521 aacatatcgt caggacaaac ttaaaaaatc tcgcacctga ggcagtgtta attttttttg
    14581 cttattcaac agaaaatgat ccaacttttc cacagcctca aaaagagcag cgtaacgacg
    14641 atcaatccgc gccaacaaat agtcaacagc tgcccggtcc acacttatcc cgcgccctaa
    14701 aaaaaactta tgcgccaagg atatcctcaa agcctcgtcc atttcgtcaa tcataagagg
    14761 gtaaaaagcc ctcacgcgag aagccaaatc atttgtggaa aaagcccact gattaaccgg
    14821 cgttgatgaa aaaaaagtca cttttttcaa ggctaaatac tgatgattaa tcaggtgaaa
    14881 aagccactcc cctggcgcct tatccgcatc atcaatgatg atctcttgtt tttccaaagt
    14941 cgatggcgag ggaagatgcg ccgcagatcc attcaatata cgcacatgat acgtgcagga
    15001 taaaatccgc gcgagggtgc ttttccctga tttttcagga ccgacaataa gcatttgcag
    15061 taaaggccac tcttcataac gggcgagcca atcacacaca tcgccattcg cctgaccata
    15121 aataagctca ttttcataga gcttgggatg atcaagggga agaagaagtt gcataaacta
    15181 ttttttaatt aagaaggaac acgtttataa aaacggcttt tgtaatattc ttctaaggca
    15241 agacgtgtaa tcgttcccac aatcgccgcc acaggcaaag caacgatcac gcccataaaa
    15301 ccaaaccacg ttgcggcggc taaaagagaa aaaataatcc atatagggtg caggccgact
    15361 ttttcaccca caagttttgg cgtcagaaaa tttccctcga acaaattcac acacccaaaa
    15421 accacaagaa ttgccataat ctgccaccat ccttgaaaat gaattagtcc gatcatcaga
    15481 cttaaaagaa gaccaattaa tgcccctaaa taaggaataa aggccaaaaa gccggtcata
    15541 aatcctacaa aaaacgcatt gggaagacta atcaatgtta gcaaaagcgt atatatcaaa
    15601 atcagtgtaa ggcacacagc cacttgacct tttgcataat ccccaagggt tttatgaacc
    15661 cgaagcgcat aatgacgcac cttaggaaga tagggccttg gaataagttt tcccacctgc
    15721 gcaataaaac gctcccaatc tttgagaaaa taaaacatca caacaggcgt taaaatcaaa
    15781 agcgaaatca cattcgcaaa gatcatccca ctgcccagaa tgttaataaa aaacccgacg
    15841 atcacctggg ccacatcgcc aacatggtgc gtaatttgcg tctttaattg atccatcgtt
    15901 ggcaaaccaa aatcctggga tagccgctcg atcagcggtg tcatcagctc aagaagttta
    15961 ttgctcatcg taggaaggct gcgactaatc acgatcaatt cctgatgcat atagggaatg
    16021 agaatcatca aaaatgtcgc gataagacag atgaaaaaaa gcatcacaac cgctgtggca
    16081 aggccacgac tcagccccat tcgcgtaatg cggctcacaa gaggattcag cgtataggca
    16141 ccgatgcacc ccgctaaaaa cggcaaaaaa atagcactta agctgtgaag aagccacaca
    16201 ctcagagcgc ctaacccgat gagtacccac tgtgtcaatg tcatggtgcg atgctatgaa
    16261 ggttttttta aaatcctaac atagtttaaa agactccaca tatagaataa tccagaggcg
    16321 atagttgtcc ccgccatgat tatacccgtc atcgaaagaa gcccttgggg gactttgagc
    16381 aaaacaaaac taatgtagag gatctgtaag caggtattta atttactaat gaaaagaggt
    16441 ttcatttgaa attttgaccg ctgcaaaaga agcatcaagg ctgtgatcaa cataaaacaa
    16501 tcacgaaaga caacaatcat catcagccaa aaaggaatcg cccctaaaaa ataaaatcct
    16561 aagtagcttc ccacaagaag aactttatcg gccaccggat caagcagctg ccctaacctt
    16621 gattcttggc ctaaacgcct tgcaaaaaaa ccatcggccc agtccgtcca tgctgcaata
    16681 aaaaaaagac taagcgccag aaattccctg gaatagaacg cacaatacaa aaaaagaggg
    16741 gtgagaaaaa tgcgaaggag agaaagtcca ttgggcaatg acacaaaggc actttctctc
    16801 catcttaaca tcaagaaaaa accttagcct acgatctcaa tctgtgaaaa gaaaaaggca
    16861 atttcaatcg cagcattatc ggtagaatcg gatccatgaa cggagttcgc ttcaatattt
    16921 tctgcaaact ctttgcgaat agttccctca gctgcatttg caggatttgt agcgcccatc
    16981 acatcacggt atttttgaac agcattatcg ccttctaaaa cctgcaccac gacaggacct
    17041 gcggtcattg agttgcacaa atcgctaaaa aaagaacgct ccgcatgcac gccatagaat
    17101 ttttcagcct gtgcacgtgt caggtgaata cgcttttgag caatgatgcg aaggccactt
    17161 tgctcgatca atgcattgat ttttcctgtg agattacgac gtgtcgcatc aggcttaatg
    17221 atagaaagtg tttgttgggt tgtcattata atacaattta aaattatcgg tctctgaaat
    17281 agttttatta caatcacaga tctttctcaa gaaaattacg cagcatcatc cgaaaccctt
    17341 tccaatttat aaatcccctc ataaaatcta taaaaccacg ctatgccctt gaaaaatttc
    17401 caaaagaaca gggcgaaaaa attgaagata tagactgaaa aatagttgtt tttctcactt
    17461 ttttcttttt taaatgcgct taaccgataa ttttgctatc attaggatgt cttagagcat
    17521 tgggaatatt cctcaaaaat taagaaatac aaaagtgata tggtgactga cataaaaaac
    17581 ctcccctcta ttcttccgct tattcccttg caaggtgcgg tgcttttccc aaaagggcaa
    17641 ttgccactcc cgattatatc gcccaaacac ttttcagctg ttgcggatgt gtggcacacg
    17701 caccgtgtca ttggcattgt tcaacccctt gatcacgaag agttgaaacg cagcccctcg
    17761 tttttcaagg caggaagtgc cgctaagatg acggatttgg gtgagtttga tgatggacgc
    17821 ctctatgtta cagtcacagg cctttgtcgt tttgatatta ccgaagacct cacgccctta
    17881 ggcgatgtgc gccgtgcccg tgttgattat tctgcctatg cgtccgatct tgtggcagag
    17941 atggatttct cccttgatcg tcccaaactt atcaaagaac tcacaacata ctttaagcgg
    18001 cttgagattg atgccgattt tgatgaaatg cacaaaattc caaacgagaa actcatcacg
    18061 gcccttgcta tggcctgtcc tttaacatcc agcgagcgtc aggctctttt acagtgttca
    18121 acgattgttg agcaatcaaa gcttttaaca tcactcattg aaatggcgaa taaagaacct
    18181 aatatgttta aaattacctg tcactaggtc ctctcttaaa aataatgatg agcaaaggaa
    18241 aaacagatgc ttgatcccaa acttctttca attctggtgt gccctctgac aaaggcgcct
    18301 ttgatctatg accgagaaaa aaatgaactt atctcaaaaa aagctggtct tgcctttcct
    18361 atccgccaca atattcctgt tatgctgatt gatgaggccc gaaaaataga tgaagagtcg
    18421 ctttgaaaaa atataaaaca ctctttgcaa ttatcactct tgcacttagc ctcggtggtc
    18481 tttacatttt atataaccat tacacctctc aaaggatacc tcctatggaa gaaaacccct
    18541 cgattgttat ttacacaacc acatactgcc cttattgtgt gaaggcaaaa aaacttctgg
    18601 ctcaaaaaaa cgtcccctat catgaaatta atgttgagga cgataaggcg cgccaggaaa
    18661 tgattgagcg cgcccatggt cgtcgaaccg tccctcaaat ttttattggt gattatcatg
    18721 tgggtggatg cgacgacctt tatgcgctag agaaagaagg ccgcctggat gaccttcttg
    18781 gaaaaaagca tcgtcaataa aagatacttt ataagctttt gtcgggtata gtgatgcttc
    18841 cctcatcatc agaactatcg taatcatcgt cgtaatagac aatagtcgcg acgtctttaa
    18901 tgctacttaa aagggcatta gtatgcagcg ataggttatc atagtcttct tcactcatac
    18961 cattttcgaa taaaacataa ttttttgctt ccttgaaggc gtcaagcatg attggtttag
    19021 caactttatt gtattttttt tctgcatcct tatacaccgt aagcaatcgc ttcatttctt
    19081 gatcctcaag gtgtttgcga aaatttaaaa agtctttgtt catgggctta aaatcccatg
    19141 taagtgacgc tcttttttca cgctcttcag catgttggcg atgctgaatg gcattataaa
    19201 aattacaata attcttgaga tgctctcgat agacaacctc tatactattt attgattcat
    19261 tattacttga gagcttagag aatacttctt ttttctcaga ttgtatttct tcattgatct
    19321 gatgataaaa attaatttgc ttcctttcag tgttagaaat ataaaaaatc gtgtcatcgg
    19381 gtgtcagcgt atcgcttgat ttggggtttt ttgagatttc tgacgcttca gagaaattaa
    19441 ttgtggaact aaggaggatt gagatataga taattgattt catgataaga accttgtaac
    19501 aatttgttac tacggtagtc tatttaggtc aaaaatatta ataattagtt aattttcatg
    19561 attatattat caatatattt caaaaataca ttacttaaac tacgcactta ttaatttatg
    19621 aaaaagttct agcttttcat acgccctgtc aaaaacccct gtacgtttta ctctaaacca
    19681 ctttttctct attctcgaag gcatacctgt caaaagctca agcgcttcaa aaacactcga
    19741 tactgtataa atatgaaaaa gaccatcacg cacagcctca acaacctcat ctttgacagt
    19801 taaattttgc gcattaaccc gaggaacaat cactccttga gaacctgtaa aaccacgcat
    19861 acgacaaatt tcaaaaaatc cttcaacctt atggtgcaca ccgcccacag cctgggccgt
    19921 accaaattga ttcacagatc ctgtaatcgc caaatcctgg cgcaggggca cgtctgataa
    19981 agacgataaa attgcacaca gctctgccat agaagcacta tcaccctcta cgtcattata
    20041 attttgctca aaggttaacg agcaggagaa agaaatagga aatgtttgcg caaaatggct
    20101 gcttaaaaac ccatcgagga taaacgctcc cttttgttga ataggccccg ccatatctgt
    20161 aaggcgctca atattaatca caccatgatt ccctacatag gtccgtgcag aaatgcgact
    20221 tggcagacca aactgatgat cccccacact taaaacggtt aaaccattca cttgcccaac
    20281 gcgcgagcct tcggtatcga tcagaacagt cccattaata atctcttgat gcacctgatc
    20341 ctcgatgcga gcgttacgca tgcgcctttc taaaagtgtt ttacgcacat ggcttttttc
    20401 aatcacagtt gatttttcat cttttgcaaa agcaagactt tccgcgataa catcgccaat
    20461 aagttcaaaa cgccccgaaa gttttcttcg gtctgacgcc cacctggcac tgcattgcgt
    20521 taaataatga acagcttccg gtgtcatatg cgttgcataa acttgctcgg ctgtttgttt
    20581 gataagacgc gaatacgcca aaacattcgg tggattaatc tcaagatcag gatcaatatc
    20641 tgctttaatt ttaaagtgaa tgcgaaaatc aggatcacta aagaaaaaag aataatacca
    20701 catgggctgg cccacgataa aaacctgaat gtccaacgga atagatttcg gcgcaggcgc
    20761 atcaattaag ggtgacggcg tatgctcatt ttggtgctct ttgatccgca cctgtttatc
    20821 acgaagcgct atcttgagat gctcccacac atcaggactg ttcgcaagat tttctgcgcg
    20881 aatcacaagg atcccctcat tggctttatg caatgagcca ggccgaatca tcgtaaaatg
    20941 tgtttcatac atccccgctt gagagatacg gtttttaatc cccccaaaca tattttcata
    21001 agagggaata gactcaagaa aaacacgtgg gtatttatat ttttgattat cgataaaaag
    21061 attcacatca taacgctcaa aacgattttt aacttgtgga tcatcctcct ccaaaaaaag
    21121 atcaatattc tctaaaacat ccgcttcaaa atcatcaatc cactcgccta aatatttact
    21181 gtaatcattg cgcagttttt gccacagagt ttttgtcaca tttttcgtaa gcgtctcttg
    21241 cagaatttta atttgcttaa ggacctgacg gcttgccagg ctcacattca gtgtcaggcg
    21301 attgagttga tcgcgaattt gagccacttg actctctttc tttttcttac tcagtgattt
    21361 caaaggagaa gctgccgcct ccccctcttc tttttcttga atttcttgga tgtgaaaccc
    21421 atccggtgtc tgttcgacaa aaaacccgta cttcagcgca aaccctttaa ctttttcaaa
    21481 atcatcattg gcttgttgat caagttgata gctaatttta tcaatttgtt taagatacgt
    21541 cggacttgta aacgtttttt tgagaaccgt ttcaatattt ttaatatgtt ctttaagagc
    21601 ttttttaagc ttacatccca tgccattggg aagtttaaaa gggagaggct tatgttgttg
    21661 cttaaaatta tttaaataga cccaatcaga gggcgctgga agtgttttaa tatgctcgtt
    21721 aaggtaatca agggttgccg tcatgcgacc acttcgatct tcacccacaa caaaaacgtg
    21781 aaaaccattt ttccgaagtt tgaggccaaa cataatggcc tctttcgcgc gtgcatgaga
    21841 ggataaatca aaaagactcg aaggtttttg aggcttaggc ggagtttccg caagaggaaa
    21901 ttctggaatg cctatttctt tatcactcag tcgatatttt tcgatgtcca taaatgtccc
    21961 tgtgatgcac agcctaaagg cgtaacccta ggattcactc taaacatcaa atattaataa
    22021 agttttagtt ttttggtttt tttaaggaaa tttaagaaaa aaaaagataa atctgagaaa
    22081 aaacttcgcc acagctcttt ctcatctttt tgcctcaggg gctttgacgg tcttttagga
    22141 gttcatgctt tggaagaaat cgctatttgt tttgctgtat ttcaatttat catgaatgaa
    22201 ttccatggct tcaatcgttc ccatgggatt cagaatacgg cgcagcaccc acatcttggt
    22261 gagctctgct ttatccgtca caagaagttc ttctttgcgt gtccctgact tattaatatc
    22321 aatagagggg aagagacgtt tatcagaaag tttgcggtca agcacaattt ccgagttacc
    22381 cgtcccctta aattcttcaa aaatgacctc atccatacgc gatcctgtat cgacaagggc
    22441 cgttgcaata atggtaagag agccaccatc ctcgatatta cgtgctgcac caaaaaagcg
    22501 cttgggacgt tgcagtgcat tcgcatccac accacctgtt aaaactttgc ccgaagaagg
    22561 cacaaccgta ttatacgcac gcgccaagcg tgtgatcgaa tcgagtaaaa tcacaacatc
    22621 ccgtttttgc tccacaagcc gcttcgcttt ttcaatcacc atttcagcaa cctgtacgtg
    22681 gcgcgcagcc ggttcatcaa aagttgaact gacaacctct cccttcaccg agcgcgccat
    22741 atctgttact tcctcaggac gttcgtcaat gagcaagaca atcaggtaca cttctggatg
    22801 attatgactg atcgcatggg caatattctg taacatcacc gtttttcctg tacgtggcgg
    22861 tgcgacaatc aaggcacgct gcccctttcc aagaggtgct acgaggtcaa ttacgcgctg
    22921 agaaaaatcc ttcccattag atccttcatg gggctcaatc tcgagcttga ggcgttcatc
    22981 cggatagagc ggggtaagat tgtcaaaatt aatgcggtat ttaacagact caggcgtttc
    23041 aaaattaatt ttattgatct tttgtgttgc gaaataacgc tcaccatcct taggcgccct
    23101 gatctccccc tcaattgtat cccctgtgcg caaaccaaag cggcgcactt ggttgggtga
    23161 cacataaata tcatcgggtc ccggcaaata gttggcctca ggcgaacgca aaaaaccgaa
    23221 accatcctgt aagatttcga tgactccatc accaataatc ggcgtatctt gctcggaaag
    23281 ttttttcaaa attgcgaaaa gaatatcctg tttgcgcaag gaacttgcat tttcaatcga
    23341 tagattttca gcaaaagcaa cgagctcggc gggagattta cgtttaagtt cttgaagatg
    23401 cataggaaaa agagagcctt tagaggttaa gaaagagagt tatttaataa aaagcagggt
    23461 ttaaaagggc ttaacgatcg caaaaatgac aataatgatg agcaaaagtg ttggaatttc
    23521 attaataatc ctgaaaaatt ttcctgtaaa gcgacagcga ccctcggcaa gttcctttcg
    23581 gcatgtgttg agaaaaaagt gaaagagaac gagaagaaaa acagctgcaa gctttgcatg
    23641 aagccacggc gaagaccatg tacctggaac catagcaaga agaatgccaa gcgtcaaggt
    23701 taaatgcatg gcgggctgca taataatttt ataaagccgt gcttccatga ccacgaaggt
    23761 ctcacgggtt tcgcgaattg aagtttcgac atgatagaca aaaagacgtg gcaaataaaa
    23821 caatcccgcc atccacgcaa tgacggcaat aaggtgaaac gctttaatcc atagataata
    23881 gtgatcgaga aaatcgtaaa aagaagtcat agctaccttt tgactgcgac aagattccta
    23941 cactttgtct aagattttaa aatctttcaa ccttaaatat gatgatgatg aatcattgtc
    24001 tttttgccat gggtatcccc aacaaagtgc tctttcattt ttgccgttgt tttttgcccc
    24061 acgtgctcgt aattttcctt gatccattta tccgccgcaa gccgtccttt ttcaaaaaga
    24121 taacttaaaa actcccactc tgtattgagc ttgctcgacc agccaaggtc ttggaaaacc
    24181 tcctcatcct cgatcacatg catatacaca cgcttaattt tttctttgtc caaaaggcct
    24241 tcatcaataa gattgctaat gaagttgatc gcgcgcatct cacgaataac cgacgcattg
    24301 ttcgtaattt cattcagacg gtccgcgatt tcccttgatg taatcggaag atgtttacgc
    24361 tctgttggat tgagctgaac catgataatg tcgggtgttt cacaattata aataagcgga
    24421 aagaaaacag ggttaccaat aaaaccacca tcccagtagt actcaccatc caccttgaca
    24481 gcattatgaa tcgttggcaa acatgcggtg gctaagaggc actcaatttt catttcatcc
    24541 aggctaaaaa ctttaaggcg ccccgtgaac acatgggttg cacataaaaa aaccttgcac
    24601 cctttaaact tgcgaagact atcaaaatca aaacattttt gaatggtatt tttcagggga
    24661 tcatgccctg tgggatttaa ctcatagggt gaaaacatac ggctaagcat atcaaaaacc
    24721 ataaaacccg gggaattata catcgtaaat ttacccatca gtttatcgat aggaccacga
    24781 ttattcaggg cgctgtgctt tcctacctga gagatttctt cccaaaaaat gcgaagctct
    24841 tcacgcgcct gctgattccc gccacgcatc aatccctgcg ccacagcgat ggcattcatt
    24901 cccccagcac tcgttcccgt cagaccttca attttaaaac ggccatcctc aagcaaacgg
    24961 tccatcactc cccaactaaa agcaccgtgg gcccctcccc cctgaagcgc aatcgccaca
    25021 ggcttttgcc cctcaatcac cgtctctttt gtttcttttt tgcttgccat aggaagcccc
    25081 agtctttcat tgatctagct acagagtagg acaagaaagt taatttttta ttaaaatctg
    25141 cagaattttc cccaaacata aaattttttt taaagaaatg gaaaagtagc ctcactcaaa
    25201 aaaatgaaat ttatcctttt gttttcctgt cacatacata aaatttttga ttccccctcc
    25261 tcgctgcaat agagtataaa ctttaatcaa gattgaccaa tccatcacct aacagtcaaa
    25321 gaaaaatgca cacgtgtctt tgaagctatt caataacatt gatttcagct atcgttagga
    25381 aaaaatgatt ctctttttga tccatgacgt cgattacccc tattgagttt aatcaagcgc
    25441 tttccgagcg ctatttggcc tatgccctct ccacgattat gtcgcgggcc cttcccgatg
    25501 tacgcgatgg attaaaaccc gttcacaggc gccttcttta tgccatgtac gagctcaagc
    25561 tcaatccaaa tagtagtttt aaaaagtgcg cgcgtgtggt gggtgatgtc atgggtaagt
    25621 ttcacccgca tggcgatgcg tcgatttatg atgctcttgt gcgccttgct caagattttt
    25681 ccgtacgcta tcccttaatt gatgggcagg gaaactttgg caatattgat ggcgataacg
    25741 cagcagctat gcgttacaca gaggcacgtc tgacagatat tgccacactt ttgttccaat
    25801 ccattgatgc tgatacagtg gatttcgttc ccacttatga cacagaagga atggagccct
    25861 cggtatttcc tgcctctttt ccgaatcttc ttgctaatgg ctcaagcggc attgctgtgg
    25921 gcatggcaac gaatattccc ccccataatc tttttgaact ctgtcaggcc atgatagccc
    25981 tcattgacgc accagaaatc cctgatgagg atctcttaaa atatattccc ggacctgact
    26041 tcccaacggg tggcgttctt gtcgagtctt ctgagaatat tgcaaaagct tatctcacag
    26101 gacgcggcag ctttcgcctt agggcacact acacaaaaga agatctcaaa ggtggaaact
    26161 accgcatcat tattgatcaa attccttatc aagttcaaaa atcaaaactc cttgagaaaa
    26221 ttgccgatct tttgcagcaa aaaaagctcc cccttttggg gaatattatg gatgaatcca
    26281 gcgaagatct aagaatcgtt cttgagccaa agtcgcgcaa tgttactccc gaacacctca
    26341 tggagtctct ttttaaacaa acagaccttg acatacgcat tagcctgaat atgaatgtgc
    26401 tgagccttag cggaaagcca gagattttga gcctcaaatc cgtgcttatg caatttatcc
    26461 agcaccgcac acaggtactt cggcgcagcg cacagttccg attaaaacat attgaagagc
    26521 gccttcatct cctcaaagga ttgcttattg cctatttaaa cctcgatgag gttatttcaa
    26581 ttattcgcga aagcgatgac cccaaagttg agcttattaa gcgttttgac ctgagtgatt
    26641 ctcaggccga agcgattctt aacatgcgat tgagatccct tcgcaagctt aatgaaattg
    26701 aaattcaaaa agaacatagc gcccttgagc aagagcaaaa aaatctacaa gatcttctgg
    26761 catctgaggc ccagcaaaaa aaatatctta aaaaagagtt tacttctctt tcaaaaacgt
    26821 atggccccga aacacttctt ggaaagcgcc gcacgctcat gagcacgccc cctgaatcca
    26881 ttgatttaaa tgttctcgag cctgttgaga ttgagcctgt tacgattgtg tgctctgcca
    26941 aaggctgggt gcgtggctta aaaggccatg tgaacgcaca tgatattaaa tacaaagagg
    27001 gagacgaagg acgctttgtc attcacagcc aaagtcacct aaaaattctt ctttttacta
    27061 aaaaaggcaa gtctttcacg ctgagctttg acaaactccc cacagggcgc ggtcaaggcg
    27121 atcctttgcg tcttttaatc gaccttgcgc cggacgatga tattctttat tttggggatc
    27181 tcccacaaga ggaaacggcg tttctcctca taaacaacaa agggcgcggg tttcagatca
    27241 acactaaaca tttagttgct cagacaaaac aaggcaaaca cattatgacg tgcgcccatg
    27301 acgaatatgt gttcttttgt gctccgatta cacagcctca cgtcgcactc ataggtaccc
    27361 accggaaact tctgatcgtt gacacaaaag acattcctca ctttgcaaaa ggacagggcg
    27421 tgagtttgca aaaatataag gacgcagagc cctccgacgc tcttcttttt gacgcagcca
    27481 atggcctgac ctaccgacgc gggctccaaa ttgtttgcga aaaaaatctt catccctgga
    27541 tggcaaaacg cggcagtgtg ggcaaactcg ccccccttgg ttttccccgc aataatcgct
    27601 tcgaagggta aaatacaagc acccccatta aaaattaaaa aaacttgcat tgttcacaaa
    27661 aaaaaagtat ataagaagtg taaagaagtc gaaattttaa ccgtttgttt acttctctgc
    27721 cgtaaagtat gaagttgtaa ggcaaccgct tattatttta aacactataa caccaaggaa
    27781 ggttacatta tgaaaattac attaaaaaca ttgatgcttc tctccacagt cgcatctttc
    27841 gcaacgatcg catctctttc atctgcaagt gcaagtgatg aattgagcgc aacaagaaag
    27901 aaagttatgc gcggtaagaa caaaaataat gaaaacacag aagagattcg catagagact
    27961 aaaaaagaaa agaaaaataa agaagagaat aaagaagaga ataaagaagt aaagaaacca
    28021 aacaaagagg ttaatttgtc cgatgatgag aatgagaaca acactgactt gggcttaaca
    28081 agaaagaaag ttatacgcgg taagaacaaa aataatgaaa acacagaaga gattcgcata
    28141 gagactaaaa aagaaaagaa aaaaattgat tcgtacgatg atgagaatga gaacaacaat
    28201 gaattgagct caacaagaaa gaaagttaca cgcggtaaga acaaaaataa tgaaaacaca
    28261 gaagagattg atatagagaa taatgaagag atcattaaag agaataatga agagatcatt
    28321 aaagagaata ataaaaataa aaaaaatgag aaagtagaaa agaaaaaaac aaacaaagaa
    28381 aaggctgctg aactctactt ccaacctgaa aaaattctca atattctcga aaaagttgat
    28441 gagttgacaa agatgcttgc aaatagcgaa aacagcatta atgagctcga aaaagcactt
    28501 cttgagaaag atatacagtt taaacagctc acagaaaggc tcaaaaaatt gggtagtgtt
    28561 gaggtggaaa agaaagaact agaagaaaaa tataaaaaat ataaaaaatt ggcgagcgct
    28621 tcaaaacttc tacaaaaagc tgaagtctcc gatgacgagg aagaagaaga agaagaagaa
    28681 aaattcaaga agaacaacaa cgaattcaac ttcgacaaca acaacttcaa gaagaacaac
    28741 aacgaattca acttcgacaa caacaatctc aaccaagaaa acttcgacaa cgagtaaatc
    28801 gttctctttg attaaattga aaaagggctc cctgcaaacg ggggccctct ttttttatca
    28861 cacccccttc atacattttt tacctttttc ccgcatcctg aaaaaagaac acttttgcgc
    28921 gggggattgc atcggtgatc gcgtcatatt aagctaagag aaacggagga aggaattatt
    28981 tttatgcagc ccacacattc ttcaaaaaat cacccctatc aaattatcga ccccagcccc
    29041 tggccccttt tggcggcctt gggtgcgcta cttttgggcg tggggggtgc tcttgccatg
    29101 catgggtata cggccatcgc cgtgatctta ggaggcgtga tcgtcgtcct tgccgcggct
    29161 gcgtggtggc atgatatgat tcatgaaagc aacaccaagg atatttatac ggacaaagta
    29221 cagctgagcc tgcgcattgg catggtgctt tttatcacgt ccgaggtgct ttttttcgct
    29281 ggtttttttg gatcctattt ttattcagca ctttcaagcg aagccctcca tggcgtgtgg
    29341 ccacctaaaa atattgtgac tcttgatcct ttccaccttc cttacttgaa cacactcatt
    29401 ttgctgcttt ccggcacaac cgtcacctgg gcccatcacg cccttgtgca aaataatctg
    29461 gaggatgcac tcaagggatt gcttttgacg atttgcctcg gtattagctt tacctgtgta
    29521 cagatttatg aatacggtca tgcagctttt ggcctcaaag acggcgtgta tggttctaat
    29581 ttttacatgg cgacgggttt tcacggcctt catgtgatta ttggcacact ttttttaagc
    29641 gtgtgcgctt atcgcacgtg gaaaaaagat ttctcgccca aagcccacgt tggcttagaa
    29701 gccgcggcgt ggtactggca ttttgtcgat gctgtgtggc ttttcttgtt cgtgtctatt
    29761 tactggtggg gagatagcgc cacatttagt cactaaaaca ctcaaaaaga tggcggatgg
    29821 ggtgggattc gaacccacga agagcttcca cccttgccgg ttttcaagac cggtgccttc
    29881 aaccgctcgg ccacccatcc aactttccta ttaatgcaag aagacacccc caaaggtcaa
    29941 ggataaaagc gtcaaaagaa gaaaaaccgc cccttgcttt caaaaacagg ctcctgcata
    30001 atggaaagga gtttttttaa gtattaaaat ttatgcgttg tacgtctttt tgcacagcta
    30061 atgcctataa tatcgataat tttgctaaat ttcttcgcaa cgaaggttat gaacctaagt
    30121 tctatgacaa tgtgattcac attcaaaaag acgaagacgg cacgagcgcg gatgtttttt
    30181 tctttcctta tggatgcatt atttcatggg ggcttgagag cgacagcgag cgcctcatga
    30241 ttgacctcgt taaaactttt gaggataagc ctttggcttc gattaccgag gatttttcaa
    30301 cctatcttat tggggataat acgcaaatta atgaggaaga agacgaaatt attctcgagg
    30361 aagataatgt tcttatcaaa ttatctcttt cccacggcct ttctcaatct gtaaaactgg
    30421 cgtcctttga ggtatctatc ggcgagacga ttgcgaaaac acgccacctt ccaggcgaac
    30481 ttgcactgaa ggggaaaatt tcactctcgc gcaaaaaact tgctcaacag cttggacatc
    30541 ttgttgaaga acgcaattcg attaatctgc acacagacat tttagatacg ccagagtttt
    30601 tttggcgccg cccaaagtat gagccctact atatcattgc tgctgaatat atggatattc
    30661 aaacgcgcct tgacattctc aacaaacgtc ttgatgtgat tcacgaatta tacgagattc
    30721 tttcaaacga gttgaaccat cttcactcat cacgccttga gctgattatt atttatctta
    30781 ttatcagcga ggttgttcta acgctcctta atatcttaaa atggatctag cattcactta
    30841 atataaacca aaatctaaat aagtaaaaaa tgttttaaaa atgagtaaag ataaaattaa
    30901 cgaaaactat aaaataattg ctcaaaataa acgagcacgc tttgactatg aaattattga
    30961 gatgcttgag gtggggatta tcctcacagg cacggagctg aaatcattac gccaaggaaa
    31021 aggaagcatt gcagaatctc acgccggtga aatggagggg ggactgtggc tctttaatgc
    31081 gcacattctt gaatacacgc atgccaaaca ttttaaccat gatcctaaac gtccgcgtca
    31141 attgcttttg cataaaaaac agctgaatcg ctttcttggg gctattcgca aaaaagggct
    31201 cacgattgtg cctgtggtga tgtattttaa tcacaaaggg cgtgtgaagc ttcaaattgc
    31261 cttggggcgc ggcaaaaaaa atgtcgataa gcgcgagtcg attaaagaac gcgattggca
    31321 acgagataaa agcagactcc tcaaaaacag agacgcttag gcatggataa gatcttaagg
    31381 gcatgcacag ttatttcgat tattttttag gcgcgtggtt catcgatcgt tgcatttctg
    31441 atggatcctc catgcggggg caagcacttt tcacgccctc tagtactaca gaaaaacatc
    31501 ttttctatgc agagcatgga acgcatgaag ggccattcgg cgtcctcgat tttttcaaaa
    31561 attatattta tgagattaaa agccctcact cttttagtct ttattttgcg gctcacgaac
    31621 aaaaaggaga gcactttatg acctttgacg gacctttagg cagcgcgctt acagccgaac
    31681 atatgtgtga aaaagatatc tatcagggta cctttcagcg cctcggagac cgtgattttg
    31741 tgatgagcta taatgtgcgc ggccccaaaa aagattactc aatcatcaca cactgcacac
    31801 gcatgcaccc tcaccgttga tttaccttgg tttgcggtca atccactcca tgtcaaaatc
    31861 tgaaaaacca aagtgatgcc caatttcatg aatcatgacg tgctgcacaa gatggtcaat
    31921 gctttcgctg ttttcctctg aaaaacgaat caacggacag cgataaagaa aaatagtatc
    31981 gggcacctca acaatgcttg tcagattttt caaaggcaga gggatcccac ggtaaagccc
    32041 caaaagatcg tatttatccg ttaaattaag actgagtaat gtcttttcat cggcaaaatt
    32101 ttccacacgg atcataatat tttgggtaaa aactctaaat tttttgggta attcttctaa
    32161 aactttataa gctaactcat aaacatcggt gaggtctgga aggttcaaaa gcccctcctc
    32221 tccatgtgtt acaccatcga ttgag
//
LOCUS       DAIAHG010000002        18373 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_2_length_18373_cov_3.298317, whole genome shotgun sequence.
ACCESSION   DAIAHG010000002 DAIAHG010000000
VERSION     DAIAHG010000002.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 18373)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18373)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18373
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2_length_18373_cov_3.298317"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5555 a         4164 c         3579 g         5074 t
ORIGIN      
        1 agcagctttt ttttcaaagc atatatattc tctgccttgc ttgatcccca tctttcgcca
       61 aacattggac ccatgaggaa tcgccaatat cgtatatttt tttacccctt tatcaatcat
      121 ctgattcaaa gagaaacgca gcaaaagaga gccaattcct ttttggcggt acgccggtag
      181 cgtaatcgaa aaataaccag aaacaatttt accgtaaaca atcatgaggc gtgacgcgat
      241 aggtttccct tgataatacg ccacataaaa gaaaacgttt gggttattga aaatattttt
      301 aacaaagttt tcaaggtttt taccatttaa accctttgag gacgcaacaa cagccaccca
      361 atctttcaga tccttctcat ccacgatgtg cttaagctca atatccttag acgctatttc
      421 tgaaaatttt ttatcgagat caagaaagcg ggatggataa atacccgaca aactataacc
      481 acctttttga atactctcta tgtgcgcttt attactctca tcaatccaaa gctcaaaggg
      541 ctgattacca taaaacgcat tcaacgtttt caaaatatcc tccagctttg cttgattaac
      601 aggtgcactg ataaacgaaa aatggaaccc tgggttttgg tgtttttcag gagctactgc
      661 agcaactccc cattcttttt caagggtttt ccctcctgtc ccacagagct ttccaaagga
      721 caaaacacac atattttcat cagacgaaga ggaattcgag gaattttctg cttgctgcat
      781 tgactggtgc tgctcactct gatttttctc cgcataacac gaggaggaga caatcccccc
      841 catacctact aaaaacacac gtagtaacaa cgagggaaag cgcactttga atctgcaatc
      901 ttttgaaaaa acacttcttt tctggtaaca ggattaaaaa atcaatgcaa gatttttctt
      961 tataaaacct ttcatccctg cccactctcc gataaaataa gaaaataatg taacctaatg
     1021 tttcttttat tctcatttat gcaaaaaatt ctcggtcttc aaatcgaccc actccctacg
     1081 ctcaacctag agtttgactc cagtctttat ctcgcctacg aggcccaaaa gcgaggctat
     1141 agccttttta cctatcaacc caaaaatctt ttttacgcaa ataatcatct taaggcgtat
     1201 ggtcaaaaaa tcgcattaag ccatgaaggc aaggcctatc acattataga ggagacgctt
     1261 tgcactctgg aagagctcga cacgcttttg atccgccaag acccgccctt tgatcaacgt
     1321 tatatggcga atctttatct ccttgagctt ttagaacata aagtccgtat tcttaactct
     1381 ccccgcggca tcaaagcttt ttgtgaaaag actctccccc ttttttttcc tgattttatt
     1441 cctccaacga tcattacaga aaatgcaaaa gcactttatg attttagtca acagcatgag
     1501 caacttgtac tgaaacccct ttttggtcat ggcggccacg gcgtcataac acttcaaaag
     1561 ccctcattgc aaacgcttga aaccctttta accctttacc acacaaccta tccagggcct
     1621 attatcgggc aaaaattctt gaatgatatt ctcaaaggag acaaacgcat tcttttgatc
     1681 caaggcgagc ctgtttctat ttttaaaaga catcctaaaa cgggggacat ccgttctaat
     1741 atggtccaag ggggcacagc agagccttgt gatttttcta agcgcgatat ggagatatgc
     1801 gcggcgttga agccccttct taagcagtat aatctttctc ttgtgggcat agatattatt
     1861 ggtgattacc tcatggaagt gaatgtcaca tcgcccacgg gattgcgcgc tgcggaaaaa
     1921 ctttacgaca tgaatcttgc tcagactttt tgggagaaca tcccgtaacg gtcaatcacc
     1981 ctgaaaaatt ttctggctaa acaccgaaat aaaccttcat aaataaacct tagcgagaga
     2041 caatcgtttt cgtctttgga tcgtagcttt gccccggaaa tagaatattt ttaacccctt
     2101 gatctgtcag ttccaccatt tgagcattaa gcggacgaag agtaatattt tcattgatgc
     2161 gacaatgatc ctgatgatag agcgtttttt ccatccaaag gctccacgat gttggcgagg
     2221 tataaatcag tgctacaagc gtataaattt catgcttttc aagggacaca tccgctttct
     2281 caggcccatc atttttttct ttaccttttt taagcgcgtc ttttatcgcc tttcgctcct
     2341 cctcatcttg aaaaagatga tgcccctcct catgctcccc ataaagggga acactcatga
     2401 gactaatcag agtcaacgcg tgacaccacc tcaacacaca cggttccttc aaaaaaataa
     2461 gatgtatcgc cttgtcttag tcttatcaaa acatcatcac tcatcggctg taaacgttta
     2521 agtgtgctat tgcttaaaac acagtaaaca ggagcttcga ataaaagcgc ttttataaaa
     2581 tcaaaaaaag tatgatccac atgggtttta agtgaaaaaa taaccgtgcg cgcgctatac
     2641 ttttccccta aagcataagg agatgccggt tttgtttgcg gattaagtgt gatgtgacaa
     2701 tcgtgcgaat tcgcaaactt ttgcgcccat ttctcaatca acgcccgttg aaagggctga
     2761 tgtaacagct cataagaccg cgttaaatcc tcacgcgcca tcaatgcctc aatcgttttt
     2821 ttatacccct ccgcctttaa gagagcctgc gtaagacttt tttcctcaga ctcaaacgac
     2881 tctttttttt caatggacca aaaaactaaa aaaaggctcc cgcccaaaaa actgaggctc
     2941 aaaacccccc acagcatcag aaacacaata ccctttgacg gcctgtgcgc ttttaaagaa
     3001 aatgtcttca ccagtgtcgt catgaggttt tttctttctt tgcactcttt tcaaaagaaa
     3061 tcgtctgagc ggataaagtt attttataat ggggaaaaag ctttttaagg tgcgttgcta
     3121 aaaaaaaact gagatcctca tgaaaataaa aacgcgcgga tgcatcctta taattcacat
     3181 ccatggcttt tggaaaaagt ttttcttttc gcacaaactc tgcaaaagat ttccaaaaat
     3241 catcctcaaa gggcacatca tgattttttt ctatggcctg aaaaaccgcc gcttccttgg
     3301 gaatatccat cgcacgcgca agagagtgtt gaaggggatc taaatgcttt ttgtcataca
     3361 taacctccac aaagagcgaa agagaaagaa aaatgatcag cagcggcaaa agaagataaa
     3421 gtgttgtctt tttaaaaaac tttaaaaaac ttgcctttga aaggttaggt gtaaggggtg
     3481 gcttaaacac tattttttgc ttacttttta agccattttg aattacttcg ttcaaatcat
     3541 agggaacgcg cttcatcgtc ataagggccg atgcttcgaa agcaaccgca tcgttcgaga
     3601 agaaaaaaac cgtaatagga gtatcttgat aaccaaagcg tttgagatat gtttttgacg
     3661 cctctagttc ttttttaaga tgagccgttt ctaaaaatgt gcgcttaaaa ataaaatgat
     3721 ttttgtgata gacagcaagc gcataacgat gattttcctg gcgaagaata aaaacccacg
     3781 aagatccttg cccatgaatt ctttgcagtg cctgaaaaaa aagatcatcc agcaagtaaa
     3841 aagacggaag acctctccct tttttgaaat tttcctctaa attctttctc agtttttcat
     3901 ccgaaaacca cacactcatg ccgttttgat acgcataaaa agcgccaaga cgccgctgtt
     3961 ctctttgaat gtgatacagt gttatatatt ttttaaaaaa agaaagcccc tgaaactcaa
     4021 ggcgtttttg ctccaacgtt tcgggcgcaa gcaacaccat agaggccgat tccagtccct
     4081 gtgcaccggg ccataatgta tacgttgaat accccatcaa ggtcgtaacg cacttttaaa
     4141 aactttcttt tatagctcat gatggcgagg aaaatcttaa aactaagtta atattttaag
     4201 aaaaataaac caaaaataga aacttcatta agattttttt tacctttaag acttacactc
     4261 taaaaaggat cccttgtgag tagagaagga aaaacagaat gaactttgat gaagcgatta
     4321 agtcccattc cgattggaaa atgaagttac aacgctattt atccaaccca gatggctcca
     4381 ttaacgcggc ccagctctct caagataatc tttgcgtcct tggcaaatgg attcatggtg
     4441 atggtaaaaa atacagtcac cttgatgaat atcaacagct tctaagcgcc cataaaaatt
     4501 ttcacaaaag cgcttcagac attgtgatac gcagagacag gggcgaggat gtcaaggcgg
     4561 atattgctct ttccggtaaa agcccctatg ctacccactc ttcgagcgtt atttctttgc
     4621 tcatgaagat gcggcaactt tgtaaaaact aaaatgagaa caagggggct aaagcggcaa
     4681 ctttgtaaaa actaaaaata gaacaaaggg cctaaaatgg cccctgcttt atttttcttt
     4741 tttggaagat gttttatttt taccctttgg cgtcgcgcca ctgagatcgt caaaaaagtc
     4801 ttttaccttt gagaaaaacc cagcagattg agggctcgag gcatgctctt catcgccttt
     4861 ttcgaactcc tctaagagtt ctttttgtct tttggttaaa ttaacaggtg tctcgacaat
     4921 cgtttccaca tacatgtccc cacgacttgt ggagcgcagc aaactcatcc ccttgccctt
     4981 aagtcgaaat tgatgattcg attgcgtccc cgcaggaatt ttaattttca cagcagcgcc
     5041 ctctatactt ggcacttcca cctcaccacc aagagcagcc gttgtcatgg gaataggcac
     5101 cttgcacgca atatcatgct cgtggcgctt aaaaaaacgg tgaggcttaa tatgaataaa
     5161 cacataaaga tcgcccgaag gaccgccacg caagcccgct tcaccgtcac cgcccaagcg
     5221 aatacgcgtc ccctcatcca cacctgctgg aattttcacc tcgatggtac gttctttttt
     5281 catccgccct gtgcccgagc aaggccggca tggtttatca atggattggc ccacaccctg
     5341 acacgccgaa caagtccgct ccaccgtaaa aaaaccctgt tgaaaacgca gttttcctcg
     5401 cccgtgacag gttttacact gcacaggtgc cgcgccaccc tcagcaccag agccattgca
     5461 acttgtacaa gggcaaggtg cggaaaaacg aagcgtcgat gtcagtcctg taaaagcttc
     5521 ctcgagtgtt atctctaaat tatagcgaat atcattgcca cgaagggctg cgtcgcgctg
     5581 ttgcgattgt ccgcgtcgcc cttgacctga aaactcacca aaaacctcat caataatatc
     5641 ggcaaagttc gccccgccaa agccgcctga aaagtcaaat ccgcccccaa agccaccggg
     5701 gttaaagccg ccctgtccgc caccttgttc aaacgccgca tggccataac gatcgtacgc
     5761 cgccttttta tcgggatctt tgaggacgtc ataggcctca ttaatttctt taaatttctt
     5821 ttccgactcc ttatcgccag gatttttatc cggatgatat ttcatggcca gtttccggta
     5881 cgcttttttc aaatcatccg cgctggcact ttttgtcacc ccaaggaggt cataataatc
     5941 ttttttactc atgattttga aaggtcagtt ctctttaatg cgcccattct acttgaaaaa
     6001 aagtttaaaa gccagatcga cctttaactc tttctttaca cttttaatgt taatgtgtta
     6061 gatgttggcg taattttatt atgtaaatga aaaacaatga aacgtatgaa taaaataatt
     6121 gcaatctctc tatcttttgt ttttgggggt ggcgtaaata gtacctatgg cgcagggaaa
     6181 gaatcagaat taattagacc ccggacacag tcagaaaaaa tggccgattt tatcagaaaa
     6241 attaatatat tacataaaaa agaaagagaa aaaatagaaa acaatatatt ccagaacatc
     6301 catcatttaa acaatgaaat cagaaaaatt aatatattat acaaaaaaga agaaaatgaa
     6361 aatgataaga agatgcttaa atcccttgaa ctacgacaaa aacaacaagg aaaattccta
     6421 gcatttttag aaacttgtga gattgagaaa ggattcatca cattcagcag gcatttacta
     6481 aatatgttgg aggaatatga aaaagatttt tcaggtgaag agatgctggc actaaaagat
     6541 gatttatata aatttttaga aaatctttat tctagttttg attttgataa agaacatgcg
     6601 caagaaataa gagaggatta ttttcattcg aactctgttg gaagcggaga tagagagacc
     6661 gacgacataa tatcagccat actggcgaat cttgaaaaaa acctgaaata ttaaaaaaag
     6721 tgaaaggaat gaaaaacaat gaaacgtatg aataaaataa ttgcaatctc tcttcttttg
     6781 tttttggggg tggcgtaaat agtacctatg gcatgaatga ggagaaaacc gaaaaagtag
     6841 aaaaaagatc tgaagaagaa aagaaggaat tttccataca caacgttgag atcctttttt
     6901 cccgatctct aagtggggat tataagaaaa aagatctgaa aaatctaaat ggttttacgc
     6961 ttcataacta tactaagatt tctatcaatc caacaaaaaa tctttattca gcgtttgaag
     7021 attttttgaa caaagaagat ataaagaaag aaattttggg gtcttacctc ttggaagaag
     7081 agattaaaaa tatcatcatt caaccagcat cattacaaat aggcaagctg ggctatctcg
     7141 gttcaacaga aggagtaaag aaatatactc aagaagatct gaaaaaaatg acagcagatg
     7201 gtgttagtaa cgatttaagc aaatctagct ataactacat atccatcgct cttttcaaaa
     7261 agggtaaaga caattaagtt tcattcatcg tgaaaaaaag cccccaaaag ggggcctttt
     7321 cattttaagt tttgccgccg cactaccctt tgttacgctt gtcgtgatca acatcctcat
     7381 agtcagcatc cacgattgtg ccttctttag gagcatcctc agcatcaccc gtcgcccccg
     7441 ctgctgcggc ttcttcctga gacgctttat aaatgtgctc acccattttc atggctgctt
     7501 gcatcaaggc ctctgtcttg gacttaatcg cctcgggatc ttctgaggtc agcacatcac
     7561 ggagatcttt aattgcgccc tcaatcaatg tgacatcctc aggcgttaac ttatctttat
     7621 gctccgtcaa ggatttttcc gtttgatgcg caagactttc accatgattg cgtgcttcga
     7681 caacctcgcg acgtttttta tccgcctctg cattcgcctc tgcctctttc accattgcgt
     7741 cgatatcctt atccgaaagt cctcccgacg cttggatgcg aatttggtgc tctttgcccg
     7801 tcgctttatc ctttgccgag acctgtacga taccgttcgc atcaatatca aatgtcacct
     7861 caacttgcgg cataccgcgg ggtgcaggcg caatgcccac aagatcaaat tgcccgagaa
     7921 gtttattatc cgaagccatt tcacgctcac cttggaacac acgaatagtc accgctgttt
     7981 gattatcctc tgctgtggag aagatctggc tttttttcgt gggaatcgtt gtattgcgat
     8041 cgatcaaacg cgtaaacaca ccgccaaggg tctcaatccc aagagaaaga ggtgtcacat
     8101 caagcagcaa tacatccttt acatcaccgc gcagcacacc gccttggatc gcagcaccaa
     8161 tcgccacaac ctcatcagga ttaacaccac gatgggggtc acggccaaaa aagcttttca
     8221 ccgtttctaa cacttttggc atacgggtca taccgcccac cataatcact tcatcaattt
     8281 ggctggctga aatacccgca tcacgaaggg ctgccttgca cggctcaatc gtgcgtgcaa
     8341 ttaaatcttc cacaagagat tcaagttttg cgcgcgtcag tttaatatta agatgctttg
     8401 gacccgacgc atcagctgaa ataaacggaa gattaatatc cgtttgcacc gccgaagaca
     8461 gttcaatttt tgctttttct gcagcttctt tcagacgttg cantgcaagt ttatctttgc
     8521 gcaaatcaat gccttgctct tttttaaact catccgcaag gaaatccata atgcgcgcgt
     8581 caaaatcctc acccccaagg aatgtatcac cattcgtgga tttaacttcg aaaaccccat
     8641 caccaatttc taaaatcgag acgtcaaagg ttccaccacc aaggtcatac accacaactg
     8701 tacccgattc ttttttctca aggccatacg ccaaagctgc agctgtgggc tcattaataa
     8761 tacgcagcac atcaaggccc gcgattttac ccgcatcttt tgtggcctgg cgctgcgcat
     8821 cattaaaata cgctggcacc gtaatcacag cctgtgtcac ggactcaccc aaataagatt
     8881 ccgccgtttc cttcattttt tgaagaataa atgcactaat ttgactgggg ctataatcct
     8941 gatcccttga gtggacccat gcatcgccat tgtctgattt aataatttta tagggaacaa
     9001 ggccaatatc cttttgcgtc atcggatcat taaaaggcct tccaatcagg cgcttaatcg
     9061 caaaaagagt attttctgga ttcgtcacag cctggcgttt cgccgcttgt ccgaccacac
     9121 gctcgcctga atctgtaaag gcaagcatcg aaggcgttgt gcgtgcgccc tcgctatttt
     9181 caattacttt tgctgtgttt ccttccatca cggccacaca cgagtttgtg gtgccaagat
     9241 caataccaat tacacgtgac atctctttat ctcctttttc ttttgttatc tgtgcgtttc
     9301 aaaaattagc agccatcgtc atagactgct aataatataa aatgaaatca cctaaaaagc
     9361 aagtacgaca tcatcacaag acttcaagtg tgagagcacg gataaagatt gatcaccgtt
     9421 tttaaacact tgttaacttt tccaagatag agtgaataat gatctctacg atagagtgat
     9481 gttaacagaa aaaataattt tatggagttt ataatatgct aaattttaaa aatattttgt
     9541 acgtcggtgc catctcctca ctatgctggg taggcacggt atctccttct ctgagcgtgg
     9601 atgagtactt taaggagatg cgtgaaaaaa gccgtggcta ttcagaaaag ccagattgga
     9661 gacgtgaaaa gaaacttgaa caagaaaaaa taaaaagatt tgtagatgat atcgttgggg
     9721 atatagaact taagatagaa aaatgtgatg ataccacaaa atcaaagatt atagatgata
     9781 tcattgaccg cctcaaagag ctaaaaaatg atgataataa tgaagaataa aaaaactaga
     9841 tttcaaaaat gttttctaaa gactaaacac ccgattgagg agatctcaca taaagaataa
     9901 acactctaag gaggtctctg caggggcctc cctttaaaaa aataaaatcc atagcttttt
     9961 tgagggtgaa aagcaagatt tttgtagcgc tttttaaaaa ataaaaacac aacatctagt
    10021 gatttttgtc tgtaaattga atagatattg tggaaaaata cccaaaagac caaacgtaaa
    10081 aaagcacaaa actgatgttt gccaatccct tctttaatga tccacgccat ccttttcttt
    10141 ctctttttcg cacgcaccac gccccaaaag cccttgcaaa agccactcag agcctcacaa
    10201 ttggccgtct tttcacctat agcattattc attacagcac ctggatgctc agccacgtca
    10261 cactgtccac actccctttg tcgctggacc acaatcaaat gaccttaacc tcagcaccga
    10321 tcatcagccc ccttcatcgc ttgccacagg aaatgcccca gcccacgcgt cacctctcca
    10381 tgaaaaagag tctcagcacg ataactcttc ctgaaaaaat gcatctcccg acacacatgc
    10441 taagcaaaac gccgatgctc aaaaagctgc cctaccccct cgcccaagag atgcttcaaa
    10501 aaacgaatgt tcagcccatc ttccaagcac ctcccacgat ccccccccaa accctcgaaa
    10561 agatcatgga ggagatgctc cctttaacca agaatacgcc tatccacaca acgcctcttc
    10621 agcaggaaaa taatatcgtg aaggacacaa cgcctttcac aacacagtct gatccatccg
    10681 tggtctccaa agaattgctc acagagacaa cgtatacaca ggataaaatt ccccctcaat
    10741 ccctcgaaaa gatcatggag gagatcggcc tgcccctttg gtatgccaaa aatacgcacc
    10801 ggtttttaga aaaagatctt tttatgccgc tcaaacagga aagcattcaa agcacctgcg
    10861 ataaggcgca agaaagtgca aagattaatg ccgagaacta ctttcaaaag ggcgtcgggc
    10921 acgcttttta tgatacattc acatcccctt ttgaagcagg gcgctatgcc catcacgttg
    10981 gccttgccct tggcgatgac acgtcccccc actttcaaga gactctgttg aacgagaaaa
    11041 taaatcagtt tcttggcgct ttgaacaacg aggtgagctc taaaaatcct gagattgcac
    11101 cgtatgcgcc cacgatttta tatcacggca ctcacaaaat tgtaaaccaa gagctcctta
    11161 gcaatggtta tgatgaaaaa gacgacccta atggccaaat caaatccttt tatcaaaaaa
    11221 cagggcattt tatctgtggc tatcactatg aaaaaaatcg caattttttt gaagaaaata
    11281 aaggaatcta ggcatcacaa cgactgaaag ctatcccccc tgtgaaaaaa ttattctccc
    11341 cactcctttt tgtttttacc cttgccttca actctctaag ccttgcaaac gaaggggttg
    11401 tccaaaattc cacggaggga agaaatcttt atttaaagac aatcgaaaaa agcaaaaatg
    11461 cctttgtcgg tacgtttaaa atttatgaca atatgtacaa tgatcctgaa atcttagaat
    11521 ccctcagcaa aagtcaaaat ccccatgtat tgctggagtc gcgtctttca aaagaagaat
    11581 cacggaatca aaaatatatt ggcatggaaa aatctcttca aaaaatcatc gacaccggcg
    11641 ctgtggtttt ttcgaatctg acactcttta aaaatagtca tatcaaatgt ctttatcttc
    11701 ctgaaaaaga gctttggata ggcacgacaa acttcgacgt gccaacagat catgagggaa
    11761 aaacaatttt gaatgaagga gaggtccgac gggatttttg ccttattctt gatgatgaaa
    11821 aacttatgaa agagtttgat attattttgc ataaagtgat tgaaaatgag acgatttcgt
    11881 ggccgacgca ctccattcaa gaggatttga agacaacgct tttatcctgg ggtcctacac
    11941 agcataaaaa agatattatt gatctcatca acggcgctca aaaatccata aaaatttatc
    12001 aacaggacct gcaagattct gacatcaaag atgctctttt aaaagcttta gacaaaaatg
    12061 tccaaattca gattcttatg tctaaacatc cctttggaga gaataaagag aacaaaagcc
    12121 tcaaaaattt aaatgaactt aaggaaaaag gggcgaatgt tattctcaca ggagaaaggt
    12181 ttaaagacgg tcactacgtt catatccacg caaaggtttt aatgattgat gatacgcgta
    12241 tgtatttagg atcggctaat ttttacggac ccacactgga tccacatgcg aatgagctga
    12301 atgtgggcgt tatcacccaa gagaaaaaat acattaaccc tgttctcaaa caatttgacc
    12361 aagattggaa agatcacgca cacatgctat gatgagatag aggtggctac ggctgtaatt
    12421 tctgaaagcg ccaaagccca cgatccagtg tataacgtat tctatgatga aaacggtctt
    12481 gatcgccaag ccaaaattca atttcgtgaa gagcaatagg gtaagcgctc cacgtttttg
    12541 gccgtggaat atccactttt gaatgcacaa gatcttgaat gcgttgctgc ataagatctt
    12601 catccaccat cactgcattt ttctctgaaa cagccgtaac aacctgcgct tcacgttttc
    12661 gctcacgcca aagacggtcc gatagagagg gaagcgttgt cgacgcagca gcccataaaa
    12721 tttacttgct gcagtgtctc acgccaccat aatgttcctg caacaaaagg atttgtagcg
    12781 atatctttgc cttttttgct ggcagagctg ctggcaaaaa taacgcccgt atcatcgatg
    12841 gtttttaata aaacaacccg actcaaaggc tttttatgcc tatccaccgt tgataggata
    12901 agactacctg gctctctcac accaaggtgt tctgcatctt tgaacttttt taatacatgc
    12961 aaagggttat gtagtagcgc atcatcctca aatcgaacga gcgccggatt ccccgtcagt
    13021 gttttaattt ctgaaacttt tgtcatctct catctcaatt tcttcgtaaa ccacagcacc
    13081 aggtcatcat caagtggata actgttacct ggaatcgtgg gttcgtcatg ataagttaag
    13141 taacgccttt tccgtcaggg atatagccac gtttgatata aagtcttttt tcatatgcac
    13201 atgttgcacg tttgggcacg gaacattgtc aaggatagca aaaacctcat accctctctt
    13261 ttgatagaaa acaacggcat cctcattaat cgtctccaaa acagcaagat agcaccctct
    13321 cacctttgct tcagattcca atctttccaa aagctttgat gctaaacctt gtttgcgaaa
    13381 gatctcgata acaaaaaaat catcgataaa gagaacatta ttgatgcaaa ttttaccact
    13441 tattccggca atcacttggt cctcttgttt aaaaacgtag cgtacaaact caaaaggcgt
    13501 atcttttccc ttaaaatcag ggacgattgt cttgttaaac tcaaaaagtt gattgctgag
    13561 cgcgtctagc tcttgtggtg tgccttctgt caaaatcatt ttttaactcc tattttatat
    13621 tgtgctcttt ggttattata acacttttcc cacagaaaca tcgtgtggat ccgattcatg
    13681 gccatcggga tcgttaaagg gcatgtccgt atagccattt ttgagataaa atttcaagct
    13741 gctttgcctg gattccacat gaatgctttg aatattgagg cgctttagcc atttttcaat
    13801 gaatgctaga aaacgacttc ccaatccttg attgcgttta ttctcatcaa tcacaatgat
    13861 acgcatcgct gccctttggt cgggccaaaa ttgaatgtgc gcatatccaa taatctccgt
    13921 accttgataa agaacaaggt gagcatggtc tttgtgatga aatgtccatg tataaggatc
    13981 ttctatgccg tacgggccaa aaaaataggt atcgcgaaaa tgtttggctg cttgccactc
    14041 tgtctcggag tgacacgtta gcatacgcgt ttgataccat ttaagaatgt aaaggcgaac
    14101 aaggcttgag aggttgtggt cgggagaacg atttttgtca ataatgctta tgatcgagcg
    14161 cactgaacag ttttctgtct ttgcaatatc cttaagagcc gcccaaaact catcctcaag
    14221 ggtcacactc gtgctgtggc cgctcaagtc aatcgatcgt ttttttatca tctacaaaac
    14281 atatctctat cttgagcaaa gagcagcaac ttttttaggg atttcctcgt aaaatgaatc
    14341 atgatgatcg atataatccc ccacaagagc agataattga tccagttgat gggtaaaaaa
    14401 gtgatcggca ccaggaataa catggtaatc aatcgcaata cccctttgag tgctgagttt
    14461 tgctgcaagt ttttcaactg ctgcaggcgt gacaatcgta tctgcgtcgc cctgaatcat
    14521 ttgaccagag acaggacagg gagcaagaaa attaaactca tagcgatccg ctggggggct
    14581 aaccgcgata aaagccttaa gctctggtcg ccgcatcaaa agttgcatac tgacccatgc
    14641 accaaaagaa aagcccgcca cccaaaaatc cctggcatgt gggttcatgg cctggagcca
    14701 atcaagggct gacgcggcgt cgctcagctc tccctcacca ttatcataac tcccctggga
    14761 acggccaaca cctctaaaat taaagcgcaa aacagaaaat cctttttccg caaaagtttt
    14821 ataaagggta tagacaacct tgttattcat tgttccacca tgctgtggat ggggatgaag
    14881 aatgagtgcg acaggcgcct ttggatcaga tgcgggttga aattttccct caagacgacc
    14941 ggcagctccg gcaaaaagaa tatcaggcat ttaggacctc ctgttcaaaa caacgttaat
    15001 ccccatagga aaagtatgaa ttaaaaataa ccaaaaagca agaatttgcc ttttaatgtt
    15061 gactattttt gtcggaatta ataagataga aaaaagaagg ggcctttgaa acatactctg
    15121 tttgaaaaaa ccctgcataa aacgacctct tgggcgtatt ttaaaatgac ggtatcatga
    15181 aaaacctttt aagtactaga tttattttga aaggaatgac cccatgcgtg taagtacgaa
    15241 agcaagatat gctgtctcag ccatggtaga tatggccgtt atcactgaaa atgatcctgc
    15301 tcgcgctgtt tccctcaatg atattgccgt acgccaagag cttcccacgg cttatcttga
    15361 gcaaattttc gctaaactgc gtcaggcgca gcttgtgcag agtatacgcg gtgcccacgg
    15421 cggctataag cttggaagac ctgtagatac aatacgtatt gcggatatta tttatgctgt
    15481 ggacaatccc tcaaaagcca cgcgctgtga tcagcattcc ccgtcggggt gtatgagtac
    15541 cggaaaaaga tgcctcaccc acaatctttg ggagggactt gatcacgtga ttcatcacta
    15601 tttgagccaa ttatcccttg aggatattgc ttttcaaaaa aaatctatac cgaatgagca
    15661 actctcgaca gattctttaa cgcaaaaggt ccgcgcctat gtattgtgat tataatgcca
    15721 cggccccttt gcgcgattct gtgaaaaaag aactcatgag cgcgtttgat cttttaggaa
    15781 atccttctgc tgttcacagc tatggtcgga atgtacgcgg atgcatcgag cgtgcccgca
    15841 acactattgc tgattacttt aaaacagacg cacagaatgt tattttcaca agcggcgcca
    15901 cagaggcgaa taatatgatt ctgaaaaatc ataaagggcc cgtttttctc tcctcgattg
    15961 agcatagttc cgttttcaat gcacgcattg atgctcaatc tattgcggtg aatgaagatg
    16021 gtgttgtgga tctctaccac cttgagtccc ttttgaaaaa tgctgagggc acaatgcccc
    16081 ttgttgctgt catggctgct aataatgaaa cgggagtcat tcaaccgatt caggatgttt
    16141 tcaatctttg taaaaaatat caagcccttc tccatgtgga tgctgttcag gctgtgggaa
    16201 agttggaaca agaaacctat aactgtgcga cgacaatgac aatttctggc cacaagctgg
    16261 gcgcactttc agggattggc gcacttatta aaaaatcaga ctaccatcca acagcgctga
    16321 ttaatggagg cgggcaagaa cgccgcatgc ggtcgggcac agagaatatc ttagggatta
    16381 tcagccttgg caaagccatc gaggatctct gtcatgacga ctggcactct actcagcaca
    16441 ttcgtgagca cctcgagtcc atgatgttaa aggctgttcc caacaccatt atttttgggc
    16501 acaaggtaag ccgccttccc aacacgattg atgtcgctat gccaggcgtt agcagcgacc
    16561 tacagcttat gttgtttgat caacagaata tagcgatatc gtctggatcc gcatgctcgt
    16621 caggaaaaat tcaatcatca cgggttttaa aagccatgaa tgttgacccg ctcctcataa
    16681 ataatgctat acgcatcagc tttggaacaa aaacaacact tgatcaggct gaggatttat
    16741 tcaaagcatg gttcggtatt tatcaaaaac atcacaaaaa ataaggtaga ttttatgtcc
    16801 gcttgcacag ttattaatat gaaaacagat caaaaaaaac acatctacct tgactatcag
    16861 gcaacgaccc cctgtgatcc acgtgtagtc gagcgcatgc taccttattt tacagagcat
    16921 tttggcaatc ctcattcgct tagtcatatc catggctgga gtgccagcga agctgtggag
    16981 gaagcccgcg cagacgttgc aaaattgatt ggtgcagacc ccaaagaaat tattttcaca
    17041 agcggtgcca cagagtcaaa taatcttgct cttaaaggcg ttggaagttt tcacaaagat
    17101 aaaaaaaatc acattattac gtgtgtcaca gagcataaat gcgttctcga tacctgtcgc
    17161 cacctggagc aagaaggatt cgagattacg taccttcccg tgcacagcaa tggccttgtg
    17221 gatcttgagg ttttgaagag tgcttttaaa gagacgaccc tcatggtgtc aattatggcc
    17281 gtgaataacg agattggggt gattcaagat cttaaagcaa taggtcagat ctgtcgtgag
    17341 cggggtgttt tttttcacac agatgccgca caggctgttg ggaaaattcc tcttaatgtg
    17401 gaggaaatga acattgattt gatgagtatt tctgcgcata agctttatgg tccaaagggt
    17461 atcggtgctc tttatgtgcg ccgtaagcca cgtgtgcggt tgaatgcgct tattaatggt
    17521 ggtggacaag agcggggcat gcgttctgga acactctctc ccgccctttg tgttggtttt
    17581 ggcgaggcct gtcgtttggc agctgctgaa atggatactg agggaaagca ccttttaaaa
    17641 ttacgcacaa aactctatga gggaattatg catcatattt cggatgcctt tttaaatggt
    17701 gaccttgaga agcgtattcc tggaaatctg aatatcagct ttgcttttgt ggagggagaa
    17761 gggcttatgg gtgacttgtc ggatctgtcg ctgtcctctg ggtccgcctg cacctccgca
    17821 tcgttagaac cttcttacgt gctgcgtgcc cttggtgtga cagaagatct tgcccacaca
    17881 tcactacgtt ttgggttggg acgctttaca acagaagaag acattgatta tacaattgaa
    17941 cgcgtcaaat gcgccgtaga gcgcctgcgt gccatgagtc cgctgtacga aatggcaaaa
    18001 gaaggtattg acataaacac cattcaatgg gatgaccact aatataagga gaatacaatg
    18061 cagtatagtg aaaaagtcat ggagcatttt aataatccac gcaatgtggg ctccttggat
    18121 aaaaatgcac cggatgtggg cacaggcctt gtgggtgcgc cctcgtgtgg ggatgtgatg
    18181 aaacttcaga ttcaagtcag ccctgagggt gtgattgagg atgctaaatt taaaactttt
    18241 ggctgcaagt ccgccattgc gtcttcctcg cttgtcacag aatggattaa aggaaaaact
    18301 ttagaggaag cggccaccct taaaaatgtg gatattgcaa cccacttagc attgcctcct
    18361 gtgaaaattc att
//
LOCUS       DAIAHG010000003        18046 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_3_length_18046_cov_2.810597, whole genome shotgun sequence.
ACCESSION   DAIAHG010000003 DAIAHG010000000
VERSION     DAIAHG010000003.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 18046)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18046)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18046
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3_length_18046_cov_2.810597"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5295 a         3526 c         3814 g         5411 t
ORIGIN      
        1 aaacttttga gcccttcgaa ctatcctgat gtgcaccaag ggaaaaagct tctccagaaa
       61 tttttattaa aatacgcttc aagaactctc gccattcttc ttcttcttat acttaattct
      121 acacaaaaaa agtctttttt gcacgcgctc ttggtatttt ttcttatcat tttaatctat
      181 tgctttgcat tcaaacatgg caccataaaa gcacagacat ctaagatgga aagtcataat
      241 gaagatattt atattaatcg cttttatctt aatgggatct agtattacaa aaatttacag
      301 ctctcactct gattttttag agaaaaaaga gagtcccccc caaacattca tgaccctttc
      361 aaaaaaagaa aatacctctg atttggtaaa ttttctatct acatatgcga aagaaaatga
      421 aaattatgat acttattttc aaaatatgct tggagattgc ctccttgaag agactcacta
      481 tgttgttaaa aatgtaaaag aaagaggtaa attatttcag ataattctag gtcactccga
      541 tatttcaaaa ctaattacaa ttgaggataa aaataaaatt cttgatcttt ttcttgatga
      601 agagagggct aaaatctatt ttcaaacaca ttttattcca gaggaaattg acattatcgc
      661 gcatcttgtg gagacatgta gcaatcatga cataaatgat aaaaggcttg atcaagccaa
      721 tacgctttat aatgtcctaa aaaaggaatt tgataagcgg ataaaggaaa aagatacaat
      781 aaaaaaagaa gccaaggaaa ataagaaaaa ttatattaat aatatcatcc cctcatgggt
      841 tcctatttta aatggactcg ctcataatat gcttggctca tggtcagagg aagactttac
      901 agaaaagccc tttatttctt atgacgcgca agaacggatt gaaaaagcgc ttcgtcgtat
      961 tgctgaaaga acgcctatta tagagttata aaaaaaggag cccacacagg ctcccccctt
     1021 tgatagcgat aaaactaaaa atcttagact tttgcctgtg ccatcacttc agcagcaaag
     1081 tcagattcct ctttttcaat accctcaccc aatgcaaaac gctcaaaggc cgtcagttta
     1141 atcgcagagc ccaactcttt tgcagcctca gtaataatat cctggacacg cttgcttgga
     1201 tccattacaa aagcttgctc aagaagaaca acttcttcat aaaatttacg cacacgccct
     1261 tcgaccattt tttcaataat cgcctctgga cggccagaag cctttgcttg gtcaataaga
     1321 atcgcacgct cacgctccaa tgacgtagga tctacatcat caatactcaa agcctggggc
     1381 tgtgttgctg caatatgcat cgcaattttt ttaccaagct cattgagttt atctttagag
     1441 gccgctgact caagagcaac aagcacccca attttaccaa ggccgggaat catcgccgaa
     1501 tgaatatagg atgtgacaac accttcagag acattgagac gtttgacacg gcgcaagttc
     1561 atattctcac cgatcacagc gattaaatgc gtcagagcgc cttgaacggt tcctccctca
     1621 agaccaacgt tttgattcgc caaatcctca acagacatat cctttgagag cgctttttta
     1681 gcggcctcaa ggacaagctg ttggaatttt tcattgcgcg caataaagtc tgtctctgca
     1741 ttcacctcaa gaacaacacc cgatgtgccc tctgttgcaa caccaacaag accttcagca
     1801 gccacacgac ctgctttttt agcagccgct gcaaggcctt ttttacggag ccagtctaca
     1861 gcctcttcaa tattgccttt tacttctgca agagcttttt tacagtccat catccccgcg
     1921 ccagtttttt cgcgcagttc cttaaccatt gatgcagtaa tttctgtcat tttttattct
     1981 ccgcttttat ctttaatatc ttaagcttct tctgcttttt cagcgattgt ttccgctggc
     2041 acatgcgcag aggccccaat atcaatacct gcactgatca tttctttttg aagaccatca
     2101 aggacagcct cagcaatcaa atcgcagtaa agctcaatag agcgcatggc atcatcgttg
     2161 ccgggcacag ggaaatgcac atcgtcaggg gaagagttgc tatcaagaat agcaatcaca
     2221 ggaataccaa ggcgcttagc ttcttgaatc gcaatcattt ctttgtttgt atcaatcacg
     2281 aaaagaatat caggaagacc acccatctcg cgaataccgc caagggcaaa ctcaagcttt
     2341 tgataatcgc gttccatgtt gagaatttct ttttttgtca tacgaccggg ctgttcacgg
     2401 agcttatcca tttccttcat acgcttaatg gattgattca ccgttttcca attcgtcagc
     2461 gtaccaccga gccagcgatg attaatataa tactgaccac agcgtgctgc tgcttcttta
     2521 actttgccag acgcttgcgc ttttgtgcct acgaaaagaa cgcgcccccc agaggatacg
     2581 acgtcacgta ccgccgtaag cgccttgtgc aacattggca ctgtttgctc aaggtcaaga
     2641 atatgaatac cattgcgcac accaaaaata taaggattca tcttaggatt ccagcgacgt
     2701 gtatggtgac cgtagtgaac gcctgcttca agaagctgac gcattgaaaa tgttggcata
     2761 gccataataa atactccaaa attaatgttt ttccggttaa acctccgcaa agtgtgagcc
     2821 ccccatcaaa agttgaagag caccggatgg tttgccttgc ctttccctca aaaaatcaat
     2881 aaggtacaac aaagcgagcc gactttgcgt gcggattcct gatgactata cagaaattcc
     2941 ctcgtggttt caaggtgaat gcgcataaat taaggatgat aacgactatt taacgcagca
     3001 aaaacaatca aagatgtgtt ttcgaatgag cttattcagg caatttgggg tggtttcctt
     3061 tgatgaggcg gctcttaaaa atgaactcaa ggaactttca tcacaatttc ttgtggttaa
     3121 tattgagggt gttttacaag cctaatacag cccttatgat ttaaaaaaaa acttagtata
     3181 aaaaaaagtg agacaatgac gacctacttt aatcttcaac attattttga gaacaaccct
     3241 ttgaaaaaag ggcgcatcct cggccttgat gttggggata aatacattgg aatgagtctt
     3301 tccgatgaac gcatggtgat tgcatccccc tttgagggct atcaacgcaa aagttttcaa
     3361 aaagacagtg actattttgt taagacagtt agggactatg ccatacaatt tattattgta
     3421 gggctccctc ttaatcttga tggcacaatg agcacaaaag caaagaacat tgtagccttt
     3481 attgagcgca ttgaaaacgt tgtcggcgtg gattttgtgt tttgggacga acgtttttcc
     3541 accatggccg tggaacgagc catgcttcaa caggatatga gccgcaaaaa acgagaaaat
     3601 aatatcgata aacttgcggc tacttatatt ctccaaggtg ctttggattt tataaaaaac
     3661 agaatctcca ccacatagtt tttaatacaa gcacagcaaa aaattacata caaccacact
     3721 ccttgaagga tggaggacac aaacgctttt gaggggtatt ctccacgagc tccaaaacgc
     3781 tctccgccaa agaatcaata aacaaccgat ccgtcccaag agcctctacg cgcgaataat
     3841 gacaaaggct cagttccttt gcacgatctt tatactgaat atcaagctcg accagcgtct
     3901 cggagtgatc ggaaacaaag ctaataggaa caaccacagc tccgagatta cttttttgac
     3961 aaaggtgtaa agcctcatca atggtcggac ttagccattt tttaggtccc acacggcttt
     4021 gatagcatat ttgataggga atagacgcaa gcaacggatc atgcataatc gcattaactg
     4081 ttgcctcgac ttgaaacgca taaggatcac cactttccac aatatcaagg ggaatgccat
     4141 gcgcagaaaa taataatttt aagggagctt tgatggtttt tatgtgattc gcaataagct
     4201 gctgatgcgc ttttataaaa tgctcattca gtggataaca gcatttcttt tgtgtaatcg
     4261 acaaaagatc tttcgcatat ttatcccact catgaaaaga gctcttcgtc gttgttgtgg
     4321 aaaactgagg ataaagtggc aaaagaataa tcgcatctgg cttagaatca cgcacagccc
     4381 taacgacttc tttcgtgaat ggtggagcat aacgcataca cataaaaacc ttcgcctgga
     4441 gccctttttc aactaaattt tcctccaaag ccaaagcttg tgcttttgtc ttttcgttca
     4501 acggagatcc tccaccgatc tggcggtaga tagctttggc tttttgcgtg cgcagtgtac
     4561 tgataagaaa ggcaagtgga tagcgaagaa gcgctggaag acggataatg tgcggatctt
     4621 taaaaagatt ccacaaaaaa ggcctcacgg ctttttggtt taaagggccc ccaagattaa
     4681 aaagaacaac ggctatcttc atttgtaaat ttttaaaaaa attaaaactg attttagtca
     4741 tcctaaagga atttcagaaa aaaatcctaa gtattctttg catttttctc tcacacgtta
     4801 atactttgtt aatattttcc tgatctaata gcaaagttgg gacacttcat aaaagggata
     4861 ctaaaatgct caagatcatc acactttcaa cgcttttttt gtcaagtata gcaacagtaa
     4921 atgctatgac aaacatggga aaaaccaatg atgagtacca agagttttta aatgaaaaaa
     4981 agaaaaaaca ataatagcaa acaagaaata cccaagccat agcgaggctc ttattttaga
     5041 tgaaaaaaca acaatgtttc ctgaaaaaca aaatgctctt tttgcaagct atgatcaaaa
     5101 taattccttt ggcaaaatcc ccggaagcgt aaaggattta aaaatagaaa aacaagaaaa
     5161 ttctcttatc aagtgtcttt ttacaacaac agaggatgat tcttttgagg ctaaaaatat
     5221 tgatctgaca tacacaagcg gtgcatccct taagttctac aacacaaaag atgagaatat
     5281 tattctttgc ttgcgctatg ttataagcta tggcaacacg atcgctaagg aaaatagcaa
     5341 tccgaccatt acttatggtg atttttgtga aaagattggt gagtatctcg gtcttgagct
     5401 aaaagtggca tcttatagct taaaaaacta accccccctc acacaacgga taagcctcgt
     5461 cagattctcc ggcggggttt ccttgatgat accatgccca aggttgaaaa caaaaggacg
     5521 ctcagccata tggctgcata tcttttttgc cgctatctcc atcgcttcat cgccctggag
     5581 gaggagctca gggtcaagat ttccctgaag tggtaaagtt gtatgggggg taatatcggc
     5641 aaaatcaaca ccttgccaaa ggccaagagc tatgggcaca tcaggcagat gctcctctac
     5701 cgctctcgca atagccacgg agagagacgc ctcaccacga ctataataaa caatcggcgt
     5761 gcttgggaaa tcttggtgaa tcgcttttgc aatcttaata aacggattga ttaaaaggcg
     5821 ctctctatcc tctgctggtg ctttaatggc ccaggaatca aaaagttgca caacatctgc
     5881 accggctttg atttgatttt ttaaatgaag agtaacctta tgtgtgagaa gatctaaaag
     5941 aacatcaaaa tctttgtcac tgcagtgatt gataatctta gaaaaatgtt cgcttttccc
     6001 ttcttcgatc atgtaacaag ccagcgtcca aggcgttcca gcaaacccga aaagggcttt
     6061 atcttttgaa agcttagcac gggtttgaga aatggcttcg tacacagggg cgagatcgat
     6121 ggaaaaagaa tcgtcgtcaa catccacgta atttttaatt ttatactttt caaggacagg
     6181 gccgtgattt tttttaaaac ttacacgttg acccagcgca tcaggaatca caaggatgtc
     6241 tgaaaaaata attgccgcat ctaaatcaaa acgggttata ggctgtagtg ttacctccgt
     6301 tgcttcctta ggggagtagc aaaaagccat gaaatcacta aaattatgac ggattgcttg
     6361 atattcggga aggtaacgcc ctgcttggcg cataaaccat aggggaaggc gcgctgtctt
     6421 ttttccaagg agggtttctc gaacgatcat gtttcttact ataatataaa tatttattta
     6481 aaaaaatgtt tttgtggtag ttggccttgt gaattctgtg gataaaaaag ttcatagcca
     6541 tttatccaca acggaaaatt tatccacaag agcgccactt gctttgaaaa agcttatatt
     6601 tttgaaggat attcttgggg ataaaaaaag aataaagagg tgtgggggaa aaactattaa
     6661 caatgaaaaa agaacgtggc aaaagggagc ggtgtgtggg gataattttt ctaattttct
     6721 gcccacactt ttgtccacag acctttgaag aggtttttca cagggtttgt gaggttgaaa
     6781 agcttaattg ttatccttga gctgtttttt gaggtcttgg atctcattat gtatttggtt
     6841 aattatttca tcgagcccat ctacctccct tggcccttga atccgtccgc cctttatctt
     6901 gagccttcta tcgtcactat acaaacttag tgttttactc cacagggagt ttcggtcttt
     6961 tttgagcttt tcaatttttt catcaggaga aagctcttca gttttctcaa agttggttga
     7021 gagatttttg ttgttgttag tctcattatc actgttgatt tcatcactac tcagcgcaag
     7081 tgttgttgtg aacaagtagg cagggtataa aataattttt tcattgtgat tagcaccttt
     7141 ggtgtatata gaatgattag tggtctaacg ctctgcgctg cttccaagca tgctccattg
     7201 aacggactga atatcaggat caaggacaag atcttccaca acttgctcga tgctttggtc
     7261 gcgcgatcct tttgttttaa agacacagcg caaagtcaca tccttgtcat ccatcataat
     7321 aatctgcatt tgcttgaggg aaaaatgcct gtggcgcatg cgtttaatga gcgtttcccg
     7381 cacatcacgg ttgtgattca tctcgcaaac gatatcaatc gagtagagga catctgcatc
     7441 aattgcaacg acagatcgcc tttccatgta aagctcaacc cgcctgagaa gtgtattcgt
     7501 gagaatgata aaaaaagtaa aaataaaggc tttgaggtgt aaaccaacgc cacaaaagac
     7561 accaagagcg cctccacacc aaagcgttgc tgctgtgttg agtccgtgga tgttaaagcc
     7621 ttctcgcata atagagccag cgcctaaaaa cccaataccg gagacaattt gtgcaacaac
     7681 gcgggacaca ctttctcctt cgacgaattc aacgccaaaa attgtgaaag cagtggagcc
     7741 aagagagaca agcatgtttg ttcgaaggcc cgctgtgcgt tgatgaaact gcctctcaaa
     7801 tcctacgagg gagcttagaa taagggcaga taaaagcctt agaaatgttt cttcccaatg
     7861 catttagaaa aaattataaa agacctttaa ttgtatttta cataaaagga agttaacaaa
     7921 atcttaaaaa aaggggagcg attcactttt taaagtgtat acgtgtttaa aattaatctt
     7981 tgaaaagagg tgttgttttg ctgagaaagg cagtcattcc ctcatgagca tctttgcgca
     8041 aaaaaagatt tttaaaagct tcgcgttcct tttgaagatg atgcgtgtga ggggtattac
     8101 tatcgtaaag atttttgaga attcttttga tggattgaat cgcgggtcct gacaaagctt
     8161 caattttttt tgcgatatca aaggcttttg aaagaagctc atcgggttta acaatagtat
     8221 tcacaaggcc ccattcatag gctttttgcg cacttataat ttcacctgtg gcacacattt
     8281 caaaacttct atgataacca ataagctctg taaggcgcag tgttccaccg catccaggaa
     8341 gaagggcaag cttaatttcc ggctgcccca taagggctct gtctgttgca atgagaatat
     8401 cccccatcat ggcaatttca agacctcctc ccaaaacata accatcgagc gccataataa
     8461 taggtttaga gagggttgat acaacctccc agggtgctag ccaaagatca attgcattgg
     8521 gatcaaggga tataagctcg tcaatggcgg cgcctacaca aaaagctttg ggattctcag
     8581 aggaaacaac aatgaccttg cagtgcttgt cactatcggc ctctttaagg tgctctgcaa
     8641 gcttaagaga aaagctttgg ctgagtttgt tcacactgct ttgaaggaga atatgaatgg
     8701 tgccttcttt tctatagctg gatatcatgg ggtgacaagc cttctaaaat atcttaagtt
     8761 tgattagatc attttttcca catctccaaa agccccttga taaagcatca tcacaattat
     8821 ttgaatagtt tagctacaaa gagtgccatc attgtggcag gggttttggt tattttaaaa
     8881 tctattgcct ggggaatgac aaatgcgctc agtcttcaag cgtcgctcat tgattctttg
     8941 cttgatgtgg gggcgtctgt tattaatttt tttgcgatta aacatgcggc tcgtccagcc
     9001 gataaagaac atcggtttgg tcacggaaag cttgaggcga ttgcgtcgct aggccagtct
     9061 atttttattg cagcgtcagc cttgtggcta attattgatt gcttaaggag gttgtatcgt
     9121 cctgagcccc ttgagcacaa tagtgttgga acggttgtta ttattattgc tgttttttta
     9181 acgcttggcc ttgttttatt tcaacgctat gtctacaaaa aaacacattc tttagccgtt
     9241 aaagcagact ctcttcatta tcaaacggat tttttaacca atcttgcggt tcttatctct
     9301 cttaatctca gtgtttcatt tttagatagc cttatcggcg gaggaattgc tatttatatt
     9361 ggcatcactt cttggactat ttttaggcat gctcttgatc agctcatgga cagagagctt
     9421 cccttagatg aagtggatcg catcgaggaa attgtgtgca gtgtcccaag agtaataggg
     9481 gttcatgatt tgcgtacgcg aatgtctgga tatacgcagt ttattcaaat gcatctggat
     9541 atggatccga atatcactct tttggaggcg catggcattg ctctagaggc aacgcgtcag
     9601 ctaaaaatag aatttcctaa ggcagagatt ttgattcatc aggatccttt tggctttgat
     9661 aatgatcatt aaatgataaa aaaaccttac cgtcttctta cctctcttca tttcaaagta
     9721 cggcattttt tgtcgaaaat gacgcgacga ttgtcgggga aagttgcctt gggtctcgca
     9781 gtgggagccc ttctttcctg cgttgcaacg tatatggctt tgtcaacgtc tgggagtttt
     9841 gaggacaaaa cacagcatgt tttgcccctt atttatcttg atctttttct tcttttttct
     9901 ctgctgattg tcattattcg gcgtcttagt gcgttgtggc gcgagcataa acaaggactt
     9961 gcgggatcaa agctgcatat taaacttgtt cttttattta gtgctattgc cgtaacgcct
    10021 gctattttag tggctgtttt ttcagcactc ttttttaatt ttggtgtgaa agcctggttt
    10081 agtgagcccg taaaggacgc tattgaagaa gcgcgcagtg tcgcccaggc ctatttagag
    10141 gaaaatcaaa gtaccattcg tcatgatgcc gccatgattg tggcggaaat gcgtccccat
    10201 gtaccagtat taattcatga ccgagaggct ttttctgaag cgctgacaga ccaagttgag
    10261 caccgcggac ttggtgaggc catcgttttt gacgtgcatc aacacgttat cgcgcgatcc
    10321 tatttaacct ttgccctgga gtttgaaaaa attcctgagg aggatatgaa aagagcgcag
    10381 cagggtgaac ttgtgattcg cgtgagcgag gggggagata aggtgcgtgc tcttgtgcgg
    10441 ctggatccta ttacaaatac ctatctgtat cttggaaaat ttgtggatgc gaaggtttta
    10501 catcacctca atcaaacaaa aggtgctgtg gcagaatatc atcgtttgga ggatcagcgt
    10561 tctggtcttc atattacttt tattgcgttt tttattctta ttgcgattat tttgctgctc
    10621 gctgcaattt gggtggggct ttctttagct tattttctta tgcaaccggt gcgtcagctc
    10681 attacagctg ctgagtcggt gcgtcagggg gatctgagtg tttctgtgcg tgacattcca
    10741 gaagagcatg agcttggtca tcttgcggat gcgtttaatc gcatgatcgc gcaagtttat
    10801 tttcagcgca gagaacttct ctctaaaaac atagagcttg agcatcgtca gcagtttatt
    10861 gagtccgttc ttcaaggggt ttcagcgggg attattggcg ttgataaaaa ttataaaatt
    10921 gaactgatta atcaaaaagc gattgagctt ttaggggtga aatcatggag tgattccaat
    10981 cgtgataaaa atttagcagc gatgagtcct gagcttttta atatgattga aaaaactaca
    11041 ggcttttcac cttatttcga gcaaattacg attgagcgta gggggcattc ccgtatctta
    11101 caagtgtgta ttgtgcccga agcaaattta aaaaatccag atgcgttgag accagaggga
    11161 tatgttgtga cctttgacga tattacatcc cttgttggtg ctgagcgcaa agcagcttgg
    11221 tctgatgtgg cgcggcgtat tgctcatgaa attaaaaacc ctcttacgcc tattcaacta
    11281 tccgcagagc gtcttaagcg tcgttatctg aaagaaatta caagtgatcc tgagaccttt
    11341 caggggtgca ttgacacaat tattcgtcag gtcagccata ttggtaagct tgttaatgag
    11401 ttctcatctt ttgcacgcat gccggagcct gaattaaagc ttgaggattt aagtgctatt
    11461 tgcaagcaaa caattgttct tcacgagggg gcgcacaagg atattatctt taatacactc
    11521 ctcccaaaac atcctgtcat gtttttgtgt gatgctcagc aaatctctca agtccttaca
    11581 aaccttgtgc aaaatgcgat tgatgcgatg gagggagagg ggggtcgtca ccaagataat
    11641 aaaattgttt taatcaaatt agtggaaaat acttctgata taaaaataac cgttggcgat
    11701 aatggcccag gttttccttt acaaaaccgt gagcgtcttt taaaaccata cgtaacaaac
    11761 cgtgcaaaag gtactggcct tggccttgcg attgttgcta agatcataga agatcacggt
    11821 ggagcgttag agctaacgac aagctctatg gggggagcgg ctgtggtcat aacgtttgta
    11881 agacagggct ctggtgttaa aactgagaca acaacgatag agaaaatctg atggcgcacg
    11941 atatactaat cgtcgatgat gagcgtgata ttcgcgatct tatctctggc atattaagtg
    12001 atgaaggcta ccaaacgcgt atggctgagg atggattatc agccatagaa gcaattaaaa
    12061 aacgtcagcc aagccttgtt attcttgatg tttggcttgg ggatagtgac cgtgatggtc
    12121 tcaagatcct tgatcaaatc aagcgcgatc atccttatgt tcctgtcatt atgatcagtg
    12181 gtcatgggac gattgaaaca gctgtgagtg ccattaaaaa aggggcgtac gattttattg
    12241 aaaagccctt tcaagtcgat cgtctgcttg ttttggtcga gcgtgcgatt gagtcctcga
    12301 tgcttcgtat ggaaaatgac tctctcaaga aaaaagcagg cccatctctc gcacttttgg
    12361 ggcaaacagc tgttattgcg agtcttaaaa atgcgcttga aaaggcagct ccgacgaata
    12421 gtcgcctttt tctaaagggg ccgcctgggt ctgaaaaaga atcgattgca aaatacattc
    12481 atgaccattc acggcgtgca aaagctcctt ttatgacctt taattgcaca tctattcacc
    12541 ctcagtttat tgaggcggag ctttttggga cagagatttc tgggctttca gcggatcaac
    12601 cccgtaaaat tggtgttgtt gagcaatgcc atgggggcac tctttttatt gataatattg
    12661 atgagatgcc gctgcctatt caggcaaaac tcttaaggct tttacaggaa aatgcttttc
    12721 agcgcgtggg cagtaatgat aaaacgcctg tggatattcg ttttattggt ggatcgaata
    12781 aaattgatca agccctttta gaaggaaatt ttcgcgaaga cctttattat cgacttgccg
    12841 ttcttgcttt tgatgtgcca gcactcaaag agcgtattct cgatattccc taccttgtca
    12901 aacacttaat ggaggatcag tcagcgcttc atggtgtttc tgttcgaaaa ttttccgaag
    12961 aggcattagc cattttacaa agctatgact ggccaggtaa tattaagcag ctaaaaaata
    13021 tgattgattg ggtgcttatt aatgcccatc catcctgtaa agaacccgtt tctattcaag
    13081 aacttcccac agacattatc aataataata atatggcagc tgtctggaac aaaggggcgg
    13141 aaattgttgt tttgccgttg cgtgaagcgc gtgaagcatt tgaacgtgat tatcttgagg
    13201 ctcaggtaaa tcgttttggt gggaatattt ctcaaacagc tcgctttatt ggaatggaac
    13261 gctcagcatt gcaccgaaaa ctgagggccc ttggcgtgta cgaaggtaaa gatgatttaa
    13321 aggatgacga atctaaaacg gcctgagcca ttgcataatt ttttttaaat acctgtgcag
    13381 acttctttaa actcttcctc aagagatgca attgctgctt taataccgtc gattttataa
    13441 tgaatatcat ttttttcatc gtctgtgagg tcagggtttt caagcttttt tatttcctcg
    13501 tctaaatcat gtttcgcaag cataagatta tgattggcaa gattgcattt ggggtttcct
    13561 gcataggctg tttgcataaa aagcattgaa atagccaaaa acaaattttt tttcatcaca
    13621 tatcttctca aaaataagta gcgcataagg ttattctacg atgaaaaagc ttaataaaaa
    13681 atgaatacat ttctttttgc aaaggggtct atcgattttg agtaagactc atggtagaat
    13741 gacgaaaact ctctattttt ttaaagagcc catgaaagtt attgttttag gtgccggtca
    13801 agtgggatat agcatcgcaa aatatctttc tcttgaggaa aataatgttt ctgtgattga
    13861 taattcacca catcttttga gaaaaatagg ggattcactg gatattcagc ctattgtggg
    13921 agaggcgtcg catcctgatg ttttgcaaag ggcgggtgct gaaaatgctg atttaatgat
    13981 cgctgtgaca gggtctgatg aagtgaatat tgtggcatgc gaggtggctc aggctttgtt
    14041 tcacgtgaaa acaaagattg cccgtattcg tgcacaaaac tatatccgtc ctgactggtc
    14101 gcatcttttt agcaaaaaac atattgctat tgatcatatt atttctcccg aagttgaaat
    14161 tgcaaagtct attagtcgta gtattcgtgt gatgggtgct tttaacgtga tttcgttgat
    14221 ggatgaccgt attaaaagta ttgggattag atgccctaaa caggccccta ttttgaacac
    14281 acctttgagg tttttagcat ctactttccc caaacttact ctttctattt tatgcattac
    14341 acgaggagaa aaggttttta ttcccgatgg agacgatcag attcttgccg gagatgaggt
    14401 ttatatcctt attgatacag agcaaatgcg cgaggtcaca gccgtatttg ggttcagtga
    14461 tttcgaggga cgccgtattc ttattatggg ggctgggaat ataggacttt ctttggcaaa
    14521 agaactcgag agtcatgccg gtattcaatg taaaatgatc gagcgtaatc cagaacgtgc
    14581 agagatggcg gccaatcatt taaaaaacac tgaagtatta tgtggggatg cgctggattt
    14641 taatgttctc aaagaagcga ataccattgc aacagaaacg gttgtgagca tcacagagga
    14701 tgataaggtc aatacccttt cgtctcttct tgcgaagcgc ttaggggcga agcgtgccat
    14761 gtgtttgctg aataatatgg cttacggggg atttgtttct tctcttgggg tggatgcaat
    14821 tattaacccc aagtccatta cggtttcaac cattttacag catattcgtc aggggcggat
    14881 tcgcggcatt cactctattc gcaacgattt tgcagagctc attgatgcag aagcgaggga
    14941 aacaagcctt gccgttgggc taagtgtggg ggatgttaat atcaatggtc aaattatggt
    15001 agcagcgctt ttgcgcgatg atcagattac ttttttgcct tcctcgaaca cgtttattcg
    15061 tattgatgat cggctgattg tcattgctgc aaaagcgtcg attaaaaaaa tagaaaaact
    15121 ttttgcgcta cgttcggggt atttgtagat tcctttgtgt cttttattta cagattttta
    15181 ataaagccgc ctttaaaata aaagaatgat agaagagctt ttagagggtg tttatgctaa
    15241 aaattcgcct gttttttgta tttttatgct ctttttctgt gcctcaagac attaaagctt
    15301 ctgaagaaaa tgagatgtgc gaggattatc atcattataa aaaaagaatg ctcaaaaatt
    15361 ttgaggagcg caacattaaa caaatgaatg atctctataa aagcgaattg aaaataagtc
    15421 gatacggggc gttggagaaa acaaatacgg aaaattatgt gttttgtgaa gttttgaggg
    15481 actatctcaa aaaaaatatt gaaaatgata atgatatggt gctgaatcag tttttagtgc
    15541 atgtgggtgc aaaggaaccg aatgaaacgt atcccgatgt gcaatggaca ggaacaagca
    15601 atcctaatga gggatggtgc acgcatattt tgagtctatc gtgcatgcca tttttctaaa
    15661 aaagcctcga aacggtggct gcttttttgg gtgttttatc aaaatgatgt gaaagacttt
    15721 gtgggcggcc aaataaatat ccttgtgcta gttggatacc catatcgtta agcgtttctg
    15781 cttcaccttc tgtctcaaca ccttcagcta aaagagtggc accgctggcc tcgagcaagt
    15841 tgagcaaaca tccaagggtg agtttttttg aagaatcaac atcgatacct tgaacataag
    15901 atttatcaat ttttatggta tcaaaatcca tataagtcag atatttcatc gtcgcatttc
    15961 ctgttccaaa atcatccaac gcaactttta taccttggcg acgcagccat gtcacagttt
    16021 tagaaagctc aataaagctt ttgggagctt tatgctctgt aatttcaata tgcagacggc
    16081 ttggatcgta tttagcgtta ataatattct taaaaagatc ttgtgcaaag gacatatcaa
    16141 gggctttggc gcaaatatta atggcgatgt gctgatccgt atgaatatag gctagatggc
    16201 gaatagcttc cataaccagc cattgattga gggagtgtgt gagattataa aaattcacaa
    16261 tctcaataca tttttctgga tgaatcgcgc caaaaagggg gtcaaaccat cttaaaagag
    16321 cttcataacc aagcacacgg ccgcttttca tatccacttg aggttgaaaa gaaagagaga
    16381 gcgggggtgt tgtttgcatt gatgtttgag gatttaaagc ctctaccaat gcatgatgat
    16441 aatgtgtatt tacaataaaa gccatgataa cacgaatatt tgttgaatca ttagttatat
    16501 actagtgatt ttttattttg caattagttt tttgatttgt ttgtttaaac ttttaaacaa
    16561 tttgaatgta tcagattcta aaatattatg caagagtaaa atccttttga gaataaataa
    16621 aaagcttctt gttattgatt tttcaaccca ctatattacc cctatgccaa atgattggat
    16681 gaccgctggt agtcatacga gaggaaagtc cgggctcctt atcaacacgg tggcggataa
    16741 cgtccgccgg catttttggt gacgaaaatg ttagggaaag tgccacagaa ataataccgc
    16801 ccctacaaag ggtaagggtg aaaaggtgcg gtaagagcgc accgcgcctc tggtaacaga
    16861 ggtggcaggg taaaccccac cgggagcaag acaaaatagg aagggatgta gggagttccc
    16921 tacacttgtg ttgatgcaag ctcttcgggt atgtcgcagg aggtgtttgg taacaaacat
    16981 cctagatgaa tggtcatcga gaaaatttat tttcttacag aacccggctt atagatcatt
    17041 tggctttttt caatcttcta tcttattttt gcaaactaat tattctataa tttctttaaa
    17101 atttattgca aaaagcctct ctggtgaatc taaaaaaacg ccctcttttg cgctattgct
    17161 ttcatcttgt aacgttgctt agctttttga gcagtgtttt tttggcaggt atttttgcac
    17221 ttcttttcta tttcggtcgt ggtcttcccg atcataatac acttgagtcc tatgatccta
    17281 ttttaacgaa ccgtatttat atgcacggag acgatatttg gcgggattat gcaaaggaaa
    17341 ggcgtttttt tacttcgatt gatttaattc ctgagcttgt tgtgaatgct tttttagcag
    17401 cagaggataa aaattttttc catcatccag ggcttgattt gatgggggtt tttagagctg
    17461 ttgtgaaaaa cacattgacg ggtaagtgga aaacacggcc tatgggcgca tcgacaatca
    17521 cacagcaagt ggcaaaaaat ttcttattga ccggtgagcg aagttttaca cgcaaaataa
    17581 aagaagcgat tatggcagta cgcattgagc gaacactgac caagcagcgt attttagaac
    17641 tttatcttaa tcaaatttac cttgggtgcg ggtcttatgg tgtgacggcg gctgcagaat
    17701 actattttca taaaaagctt aatgaactca cagtaggcga ggtggctttt ttagcagcgt
    17761 tgcctaaggc tccagaagtc tatcaacccc ataaaaatcc aggtcaggca aaaacacggc
    17821 gtgattgggt gttggatcgt ctttatgaat cgcatttgat ctctgcaaga gaacttattt
    17881 tagcaaaaga taaacctatc tcaacaattg caaagcataa agaagaaaaa ttccacggtg
    17941 agtattttat tgaggcaaca cggcaaaaac ttattgagct tgtagggttt aaaaactatg
    18001 agaagggtgg ctattttctg tggacaaccg ttcaaaaaga tatcca
//
LOCUS       DAIAHG010000004        17884 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_4_length_17884_cov_3.309710, whole genome shotgun sequence.
ACCESSION   DAIAHG010000004 DAIAHG010000000
VERSION     DAIAHG010000004.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 17884)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17884)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17884
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4_length_17884_cov_3.309710"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5129 a         4061 c         3597 g         5097 t
ORIGIN      
        1 gtcagccgcg caagcgttac gcaacgcctg cgtctgcccg gaaaattagc ggattgttct
       61 gatacacagc tcaatgaaac ggagcttttc cttgtggagg gtgattctgc gggaggctct
      121 gcgaaacaag cgcgcgaccg aaaaacccag gccgttctgc ctttgcgggg aaaaattctc
      181 aacgtagcga acgcaaccct tgataaaatg aaggctaacc aagagctttc ggaccttgcg
      241 cttgcccttg ggtgcggcac gggcaaacat tgtcatgctg aaaagcgccg ctatggcaaa
      301 gtcattatta tgacagacgc tgatgttgac ggcgcccaca ttgcgtcgct actgatgaca
      361 ttttttctca aagaaatgac cgaccttgtg attaaagggc atctttatct ggcacagccc
      421 cccctttacc gcatcacaca aggcagcaca acctattacg cagcgaatga gaatgagcgc
      481 gatcttcttg tgaaaaaact taaaggaaaa ccgcaagttg gccgttttaa aggccttgga
      541 gagatgttac ccgctcagct gaaacaaacc acgatggata aaaaaaatcg cacactcctc
      601 aaaattaata ttcaatccct tgaggaaagc acacaatttc tggaaaaact tatgggaaaa
      661 aaccccgaag cccgttttca ttttattcaa gacaatgcgc tcaaaataag cgatggcccc
      721 tttgactaat cacaggggaa gttgtttatt tatacactct atagcttgat ctgattctct
      781 aaaaattctt taaatgcttt gactttttta gaatcattca ggtgatcagg gtgcgtaatg
      841 tgcagatcaa acgacggccc cttacactcc ggcaaaacgg gaatcagttg attaaaatcc
      901 cgcgccacaa agtgaggaag cgctccaata cctgttcctt tttcaatcaa ctgcataatg
      961 gcataagcgt tattcaccgc cacatgaaaa tcacgcacat gccctgtctc agcgccgact
     1021 ttgagcgtcc aattcactcc ctcaaacggg ggcgtaatgt gcgttccaaa gacaataagt
     1081 ttgtgataat ccaaatcctc aggatattta ggcgtcccat aatcaagaag atattccttg
     1141 ctcgcataca tctgaagcgt atccctaaca aaaaaactac tcgtcatatt ggggtgcttt
     1201 ggagggccat aactaatcgc gatatccgcc tcgcgcatgg aaaaatcaac ttctgtatcc
     1261 gtcaaggcaa gacacaaatt aatgcctgga tacatttctg aaaaaaggtg caaacgaggc
     1321 ggcatccata tgcttccaaa tgctacggaa caggcgacgc gtagctcacc tttggcctca
     1381 ttacggctct caagaagacg gttttgtgtc gcagaaattt gagcaaacac actcatgact
     1441 gttttatgca aaagctcacc ttctgcggtg agaatcagcc cccgcgcatg gcgtgtaaaa
     1501 agttgcgcgc ctaagttttc ttcgagcgta ccgacctgtc gactgactgc agactggcta
     1561 atattcagac gctcacccgc ccgggtaaaa ctgccagctt gtgccaccac atgaaaaata
     1621 cgcagcttat cccaatccat cgcactcata aattataaaa ccccttgttc tgctaaaaag
     1681 cgctctgcat caagtgctgc catacacccc tgccccgccg atgtcacagc ctgacggtaa
     1741 agaggatctt gaacgtcccc tgctgcaaaa acccccggaa tcgatgtttt aggtgttccc
     1801 ggctgtgtca aaatatatcc ctctttattc aaatccaact gaccttttaa aaagccactc
     1861 atcggtgtat ggccaatcgc cacaaaaaca ccttccgttt caagggtttg aaggcgtcca
     1921 tccactgtat ttttcaacac aacacctgta acatttggag gattatcatc gcccaaaatg
     1981 tcctccaccg tgtgattcca cagcacagaa attttaggat tattaaaaag acgcgtttgc
     2041 gcaatttgct ccgcacgcag tttatcacgc ctatggacaa gcgtgacatg agaggcatgg
     2101 tgcgtcaaat agagtgcttc ttccaccgca gcatttcctc cgccgacaac caccactttt
     2161 ttatttttaa agaaaaaccc atcgcaggtt gcacatcctg acaccccata tcctaaaaat
     2221 ttttcttcac taggaaggcc aagccatttc gcctgcgcac ctgtggcaat aatgagacta
     2281 tcacaggtat aaatttctcc gccctctgtc tgacaggtaa aggggcgttt gctaagatca
     2341 acacgttcaa cgatatcttc gatcatggtc gtcccgacat gctccgcctg ggcacgcatt
     2401 tcttccatca gctgaggccc ctgaacggct gtgcgaaagc ccgggtagtt ttccacatcc
     2461 agtgtaatca taagctgccc ccctggctgc atcccaagga actgcagggg cttgaggttc
     2521 gcacgcgccg cgtaaatagc cgccgcatac cccgccgggc cacttccaat aatcatcact
     2581 tttgcatgca ttaaagaggg tatagtcata aatttttcac atagcttcat tttcttcttg
     2641 gcaacctagc acaaatgctg atatatatcg agtcattaat agggccggct tagctcagtt
     2701 ggtagagcaa ctgatttgta atcagtaggt cgcgagttcg aatcctgcag ccggcaccat
     2761 cttcaaattc ctgttgacgt catttaaaag atgccagaaa atgtcatccg tagatgtctc
     2821 tcttacaggt ttccattatg aaaaaattcc tgacactttt tacttttatt atgttcgcag
     2881 caaaagcttt tggctcagaa aaatcctctt gggaggatgt tatgaattca aaacagaagc
     2941 aaaatctgaa atcgaatgac gttaaagaag cgatctcata ttttaaagat gatgtcctca
     3001 acaaaaagct tgagggactt ggtgggtgtg agattacgaa tttttctttt gaagtggaaa
     3061 attttaataa cgcaaaagtt tcagcctatc ttcaaatctt aggggatacc cgctattatg
     3121 gatctttgga ggaatcttat aaacttggta ataatgcaca aggttggcgc caactcaccc
     3181 ttgaaattgt taataagaac tttgaggctc taggtaagcg aataaaagat tttggcacaa
     3241 acgatggatc atttagttca gaacgtgata atgcaaagcg tcaaataaac aaggcttaca
     3301 agaatttttc cccaaattcc tggaagatct tgcttaacgg ttacgtggat tgggtttctc
     3361 agcgctggaa agatcttcaa aatttaatac agtaaatcta aaccctggtg gcccttgttg
     3421 gtttcattga gaggcttata ttatgaaaaa aattattata cttgttatta tgttcgcagc
     3481 aaaagctttt agctcagact acgagtttga gtcttatgat tattctgcgt ccatacaaaa
     3541 gtatgataag gaaaaagttt ttcattgcct gagaaaatta ggtgaaagcc attattacga
     3601 aaacttaaag gaggctatga aggagacaga ttttacgaat cgcacaaata ggatagaacg
     3661 tcttttcaca aacctgcgag ctcttaataa aaaggtaata gaatttgacg agggcaaaaa
     3721 tcttctcgag aatgcgcttt attacgttga tgatttgtgc acaacgtttg aaatttggcg
     3781 atccttgtac acttaataaa aatgaggctt aaattttaac gatgatcgtg atcccttctc
     3841 ttccttcctc tgtacgtctt gtggccgtca ccaaagggcg ctctgttgct gagattgagg
     3901 atcttatcgc agaaggcgtt atatggtttg gtgaaaatcg cctgaacgag atacaaacca
     3961 aatggccttt tttaaaggaa aaataccccc atgtgcgcct gcaatttatt ggtcagcttc
     4021 aaagcaataa agtggcaaga attctcaaac actgcgatag tcttctcagt attgatcgca
     4081 aaagccttct tgaggcaatt gcaacagctc aaaaaaatga tacttcttca aaagaatatc
     4141 tcattcaggt caaccttgca aaagaacctc aaaagggggg agtgatcccg gaaaatctca
     4201 aagagctcat cgtttgtgca cgcagccaca cccttcccat tcaggggctt tccgcgatcc
     4261 ctcccctgcg ccaagacccc accccctatt ttacccagct caaggcgctt tgcacgctct
     4321 acaatcttaa agagtgcagc atggggatga gtgacgattt tgacacagcc atcgcccttg
     4381 gggccacaca agtgcgtctc ggccgacggc tttttcgtta gagatcttcc tcttcctcgt
     4441 taagatcttc ctcttcctcg ttaagatctt cctcttcttc gttaagatct tcctcttctt
     4501 cgttaagatc ttcctcttct tcgttaagat cttcctcttc ctcgttaaga tcttcctcaa
     4561 gttcgccttc tatctctcgt atctcttttt ctaatttttt ttccttgcgc ttttttttat
     4621 ggccagcaac taaaacgcca ctgtcatcaa ttttttcagc gatagtgtta agctcttctc
     4681 tgatttcccc tagaatctca gcactttctt ttctgttatg gtgttccttt ttaatgagta
     4741 attttttgag ttctttttct ttgatttgcg tgtctagcaa tttttcttta atgtctttca
     4801 cactttcaag tctctcttcc ttcttattca ccttctcttt cagatcaaca atctcttttt
     4861 tttctttttt attgtctttt ttcatatttt tttctttttt aggaaattct tttttgtcca
     4921 gatcctcgac gatagtctca ttcttatttt tttcagaaga ttttttttct agatcctcga
     4981 cactagtctc attcttattt tttttattta gtggttttct ttctaccttt tgccttgtag
     5041 agttagagtt ctcattagaa aaaccatcag tgcttgtgag caaaagcatt gcggaaaggg
     5101 cactcgcaca ctttaaaaaa tttttaatca acataaaatc tcctttgagt taaaacaatt
     5161 caacatcatc actggccttt gttgaggccg tttccatccc cgatgaaaaa ggttctttgt
     5221 cctgagttgc cagaagatga tcaatttttg accccatttc ctcgggcaca tgatcataga
     5281 gcgcttgcgg atgcaccgca caatggggga tgtgaagctc atcttgcagc tcatgaatcg
     5341 tttcaataga ttttttcatt tgctcaagat cttgacgcac gcggtcctga tactggatgt
     5401 ctgtgataag ttgacgcgca gaatccgaaa actcacggga agcctggctg gcatttgtca
     5461 cgatattgga aattttctca ctattggcaa tgagaccttc catcatctcg tccagctgat
     5521 ccttcgccat catattgctg ctcatatcaa tgctggctac tttttgtaaa atttcttgag
     5581 cgtaattaag tgtctctgtc atttgattga cttgactacg gatactaatc gcaaggcgct
     5641 gcgtatcatt ggaaagatca cgcacttcac tcgcaacgac ttggaaactc tcgcccgcct
     5701 cccctgctct cacggcttca atcgttgcat taatagaaag atattttgtt ttgtggttaa
     5761 taatttcaat ttgacgaatg cttttttcaa tttcattcag gtttttcaca ccatcattta
     5821 agatatacac catcaacatg gcttgctttg aaatctcaag catacagcta atcgattcaa
     5881 gaaatgtttt gtgcagaagc gacgaaactt cttggatccc gatatcgctc tcgcccacct
     5941 taacggtttg tgcaatagag ataagctgtt gcaaataatc tgcctgcaag gccgcatttt
     6001 ttgaaagcag ttgaaattga ttcgaaagtg ccaccgtttt attttcagaa agattcatca
     6061 cagcatcaat acggtgcgac atgccttcat ttgctttttc aatgacactg tgcagctcgg
     6121 ccagaacagc ctcctcctct gtcacgtgat ggggaacgtg atgaggtgca ggatgctctt
     6181 cagaagcttt tttgacaacg tttaagtcga tatcacttgt aatcatacat agcactcggc
     6241 gttttagaac atgacaattc tgtctttagt cctattaaaa caaaaaattc tgaataaaat
     6301 attaataata cataaaatta attttatatt tttgagatgt tttttgagcg ctgtatattt
     6361 atcgagactc ctacaaaaaa agactttttt taaaatattt tattaaaaga caaaatagtc
     6421 tatatttaga ctaatttttc taccaaactg cgcaaagact ctatatgcac tttttttggt
     6481 cacagcacac ccccaacttt tttctctgct tttttgccag catcgttatc aatctatttt
     6541 tcttctctcc atccttggct gagacgcctt ctcacacaaa cgaaccgcct gcaaaagaac
     6601 atacgcatcc tgatccagaa aaagagctag aaccccttgt gtgcgataca acaccccaag
     6661 ggctcaccct ccttgtcacg ggacttggcc ttaatgaatc cctcacagag cgcgccattg
     6721 cagaactccc caaagagatt gctctctcat tctcagccta cgctacaatg atcaaccagt
     6781 ggacatccct tgcaaaagca gcaggtcatc agatttttat tgagcttggg tcagaaaaag
     6841 acctgaataa cacagggatc cttgcttatt ttgatggtgt tcttcttatt cctgaaaaac
     6901 cgcccgtgac agatcctaaa atccttgaaa atttcgtcta tagccttgga gtcaggaaaa
     6961 aaatgatttt agaaggtcgc ctctttccca aagatttatt agcactcgaa gcccaaaagc
     7021 aaaaagcccc ctttagaaag attcaatact ctctttccga agcaagtctt tttgagggaa
     7081 aacaagatat ttttgagctc aaaggcattg tcacctttcc tgttagtttt cttaattatt
     7141 tgatcgacat gttaaaagca cagaaaaatt tatctcttat tcccctcacc ctttgaggcg
     7201 ctatgcaatt aaaacttaac aggcgggttg ttttcgttta cactaaaata cgtgagtaaa
     7261 gatcgacccc atgctattga tgtaacaatt gacaatgatc aatggtgcga tcttggtcat
     7321 ctatcaaaag acttaggccc tgatcaaaaa aaatgggatg aggttgcgtg gacttctttt
     7381 tttgagagga ttctgttaaa agtcgccgct gtcttagggg tagacaaacc ctttggtcta
     7441 agtattcttc tgacagataa tcaccatatc caagagctca atcacaattt tagaggtaag
     7501 aacaaaccta caaatgtgtt atcctttccc tcaggcattg aacatccttc gccggatgga
     7561 tttttttttc taggagatgt ggcctttgcc catgacgtta ttgttgaaga gtcaaatact
     7621 gagaaaaaaa tatttttaaa tcatatgaca cacctaacga ttcacggtat ccttcatctt
     7681 cttggctatg atcacgaaat accagccgag gctgagacaa tggaaaacct tgaaattctt
     7741 atcctgagtc attttaatat taggaacccg tacacgtaat ttatgagaac tatgtccgat
     7801 acactatccc ccctttcgcc aaaaccccat cgcctgaaag gaaaaatgcg tcagctgtgg
     7861 cgtaaactca cccgtcatga cgatagcagc cttcgtgaaa ccctcgagga gcttatcgaa
     7921 gaagttgacg aggttgaacc ctctattgaa tcggatgaac gacttctgtt aggcaatgtc
     7981 ctgagtctga gggatttaac cgcccaggat gttatgattc cacgcgcgga tattgtggcc
     8041 gtttcgcaca cgattaatgc gcaagagctt atggccacaa tgacacgcac aggcctttcg
     8101 agagttcccg tgtacaaaga taatcttgat gaaattattg gcatggtcag catgaaagat
     8161 atgctggctt ggtgccaaaa taaatccaca acgtttaaaa ttcgtcagat gcttcgcgaa
     8221 gttctttttg tctctccaag catgcgcacc ctcgatcttc ttcttcaaat gcgccaaagc
     8281 ggtacaaaaa tagcgcttgt cgtggatgaa tacggtggga ttgatggcct tgtgactttt
     8341 tcaaatttga ttgaggaaat tatcggcgat atccaaaatg cgcacgatca gggagaaccg
     8401 atgaaaattc aacgccttca ggatggaaat attcttatcg atgcgcgtta taatctagaa
     8461 gaacttgagg agcagttgaa ccttaatttc gatgtcgcgg atattacgga cgacgttgat
     8521 acaattgggg gccttgtgag ctctcttgca gggcatgtac ctgttccagg ggaacttgtg
     8581 cgccatccca acggttttga atttgaagtc atggatgcag accccaggcg gattaaaacc
     8641 cttcttattc acattacaag tcttaaaaaa taatattcag cagagaaaag gccttttgac
     8701 acgatgacac agaaaatgaa tgttgcgatt aacggttttg gacgcatagg ccgtatggtg
     8761 ctcagagctt ttctcgagca ggatagcctt ccttttgaga ttattgcgat taatgacctt
     8821 acccctattg agacgaatgc gcaccttctt gcgtatgatt ccgtgcatgg caggctgaag
     8881 gccgatataa agattgagaa tgatcacctc gtgattcaaa agggttcaaa aacccacact
     8941 atccatgcgg taaaagaagc aaatcctaaa aatcttccct gggccaagca ttcaattgac
     9001 gttgtgcttg aatgctctgg caggtttacg gatggtgaat ctgcaaaagc acatcttgag
     9061 gcgggtgcaa agaaggtttt tatctctgcc cctgcaacaa atgttgatgc cactatcgtt
     9121 tttggcgtca atcacacaac actcaaagcc tctgatcgga ttttctcaaa cgcctcatgt
     9181 accacgaatt gccttgcgcc tgttgcaaaa gtattgcacg agcacttcgg tatcgagcat
     9241 ggttatatga cgacgattca tgcgtataca gccgatcaaa atcttgtgga tggcgcccac
     9301 aaagaccttc agcgcgcacg ggcagccgcg atgtcgctta tccccagcac aacaggtgcg
     9361 gccaaatctg tgggccttgt cttgcccgat cttaagggaa aacttgatgg cacatccatt
     9421 cgtgtgccga caccgaatgt ttcgctgatt gattttaaat tcacgacaaa aaaccctgtc
     9481 acaaagacag atattaatga agcgatgata aacgctgcga agggtccttt gaagggaatc
     9541 ctcgcaacca atagccttcc ccttgtctcc atcgatttta atagtgatcc ccacagtgcc
     9601 acttttgatc tttctcaaac acaaaccctt ggccatcatt ttgcgcgcat ccttgcctgg
     9661 tatgataatg aatggggctt ttcaaatcgc atgattgatg tgctaagata ccttaagacg
     9721 ttatagtttt ttttaaatca aggagatttt ctcatgagcc gttttcaagg aatttttatg
     9781 aatcttggcc tgggtgttgc cttagcaacg ggtcttgtct actccagcat tgttgtcaca
     9841 aaatccatgg agcgtatgcg cagcaccatc gaagtcaagg ggtatgcaga gcgcaaaatc
     9901 aaagcggatt atgtcagctg gaccattcaa tttacagcgc gcaatgctga cctggcccaa
     9961 gcttataaaa cccttgagat ctataaggat gaaattcttg gatttttaaa agcaggggat
    10021 gtcaaggatg ctaagatcga catcggtggg gttaataaac aaacacacta caaaggcgat
    10081 gaaaatggtc gcagcaccag tgatattgga tcctttgaac tcacgcaaac agtgaccatc
    10141 gcctcgcctg acattgataa aatcaaagag cttgcgctta agattagtgc tcttggggct
    10201 aaaggcattg aaattgaacc acgctcgccc tcttatctta ttggacggga aaaacttgag
    10261 aaagttaaga ttgagctcct tgctgaggcg acaaaaagtg ctaaggagcg tgcggatcaa
    10321 tttaccctta atagcggcac aaatattggt cgcctcatca gcgcgcggca aggcgttttt
    10381 caagtcacag gcgaggattc gacagaggtt gatgactatg gaacctatga tacatcctcc
    10441 attgacaagg ttgtgaaaat cgttgtgacg ctctcgtata caacagaaag ctaatccgtt
    10501 taacataatg gagtaaggaa tgcaggggat tcttgcctct tggacgaaaa tcttttagca
    10561 tcagccatgt tttaagcaat aaggtctcct gattcatgcg tgaatttttc tacaaaactc
    10621 aaattggcca aaaatttctt gacaggccgt ggtatggata caacgtgtac caggcggcta
    10681 agctcgcgga acgccttcat attcctgaaa ttgcggtgat tgagtttggt gttgccacag
    10741 ggcgtggcct tttgtgcatt gaaaagcatg tggaagcgat tgaaaaattt tcccctgtaa
    10801 aatttcatat ttatgggttt gatacaggcg gcggtcttcc cacactcgag gggtataaag
    10861 atcttgccca tcattggaaa gcaggcatgt tcacaatgga tgttgagggg ctccaaaaaa
    10921 aactaaaacg cgcaaaactt attcttggga atgtgcgcga aacggtgccg cagtttttgt
    10981 taaagcacac tccacccccg attgcgtgtg tgctatttga tgtggattta tattcctcca
    11041 cccgcgatgc attggctctt tttcacgaaa atcactctgc ttttttgcca cgcgtacgca
    11101 cttattttga tgatatttta ggcaatgaac tatcgcttaa taatgaattt ttaggagaga
    11161 agtgcgccat ttctgaattc aatcaccaac aagaatcgat taaaatcgcg caagttgcac
    11221 accttcaggc gaaaagcatc cgctataaat ggtacagcaa atgctatgct acgcattttt
    11281 ttgagcatcc actgtatgaa aagtatattg ggcgctcgta aaccttaggc ccaataatta
    11341 aaaaaatttt acccagcggc tacaacttta tcctttacgg aaatccccga caagagcggt
    11401 gaccacgcag gatccgaagc attgtggggt gttttaagac tcacttttac aagccccgtc
    11461 atatccatac agcacagctg tgagcggaca tatccccctc gctgtggcgc gtgctctttt
    11521 gtataggtaa tatagcgacc attaggtgac caagcggctc cctcaattaa ataagcttcg
    11581 tcaataagac gttcatttga gccatcaatg ttcatcacac ccagataaaa acgccccccg
    11641 atttgcttac taaacgcaat cagatcaccc cttggcgacc atacgggctg agagtatttt
    11701 ccaggcccct gactgatttg cgtgacacca ctaccgtccg cgcgcatcat aaacattttc
    11761 tcgaaaccat tacgatcaga agtaaagacg atgtattgtc catccggcga ataacacggc
    11821 gacgtatcaa tacgcgtatg ctgtgtaagc tggcgtgttt cttttgtctg aatattatag
    11881 gtatagaggg ctgtagttcc atttttagac aaactcatca caaccgtcga cccgtctggc
    11941 gaaaaacgcg gcgcaaaggt catgccctgg aactcgccaa gaagttttgt ttcttttgcc
    12001 cgcacatcca ttaaataaac gcctgcactg tgcttattat aacgcaaata cgcaatatga
    12061 tgggtcgagg gtgaaaaacg cggcgtgagg acgaggtttt ttccatccgt caaatgcaca
    12121 acattatgtc cgtcaatatc catgatgctg aggcgttttt tcttggcttt accataacct
    12181 tgctcatcaa taaaaacaat ttgtgagtcg aaaaagccag catcacctgt gacacgctca
    12241 tacaccacat cggcaatcat atgcgccagc ttgcgccact ttccctcttc tgcggaaaga
    12301 ctaaatcctt ttaagagaat ccctttgagt acatcataaa ttttaaattg caccgtaatt
    12361 tttgacccgc tgtgttcaac tttaccaatg agcaaaattt gatcattaat cacacgccag
    12421 tctgcaaaac gcacatcgtt atgcacagac acagggtctt gaataaatcc agcacgactc
    12481 aaaagctcaa agcgcccgca cattgcaagg tcgtcctcaa tcacttgcgt aatttttcgc
    12541 gcaaattcac cctcacccag aaaaagagga atggcaatgg gtgtgggttt gatttgccct
    12601 tttgtgattt cgatcacttc ggggatgtcg ccggatgatc ccgctcctgg tgcagcaggt
    12661 gccccgggaa gagttcccgt gggaagattc ccaagaggtt taggagcctc cgcaaatggt
    12721 gtagaggtca cacacgaaaa ggcacccaaa aaaagaaaaa ttttaaaaaa catggggaaa
    12781 gaaaaataat attttatctc ttctttttta acccatgcgg gggggcttcg caacgctttt
    12841 tcatcctcgt ctcaatgatc aatcaatgtc aaaatcctca attttgttat ttttatcgta
    12901 atcgcctgtt tgaacaagtt tatacatttt ctgatagctt gttatctttt tctccgtcag
    12961 ataattcaaa aacgtctcaa ggtcctgtgg cggaagcaca aaaaggagat aaaggaattt
    13021 atcaaaactg taatccccgc tatgaagatt attatcatct atgaacgttg agcgtctctc
    13081 ctcaaaaacg ctactctgtc tgtggaggtt tacagaggaa tcatttttta ttttttccca
    13141 ctcagtggtg ttaaaatctc tgatcgcatc gtcaatacta atcttcttgt tttccatata
    13201 ctcacagcga tcgacgatct ttttgaacaa agaatcccac tctttttcag ggtgaagcga
    13261 aagaatccat gtcagtttgt cagcatagaa atcagagctt tgatccccga gaagcgaata
    13321 agcatagttt tttttatttt cagaatattt tttccaaaaa tctttattat tatcatttga
    13381 attaagacgc ggaggttttt tgcttttaat aaattccttg gcaccctcat aattttttaa
    13441 aaatgtgtca gggagcggca cactattttt aaaatactcc cccccaatcg aaagggtcaa
    13501 ttttttttca ggcgagaagt cacttatgta cgcaattgga agttctttaa gaataaaatc
    13561 cagttcggtc gtattttcag gaatttctac ttttctacca acaaacagct gacccgaagg
    13621 aagcgaaaaa tatccattcc ctaaatcttc acgggaaggt agtgcgaatg taaactcagc
    13681 attttcccat ttcttaattt cctttgatat ctcttcatct gagagcgtcc tttccttttt
    13741 attttcaaga ccaataaaaa gcccaggaaa tgccttctta agctcatcct ttgtaaaaga
    13801 tttgttgtta accgtaaaag ttgcggatga agattcacca atatcatgaa gttcttcatc
    13861 cgtagaattt gatgcaaagc atatcgaaaa agcagtgctg cccaagagag cgacggcgga
    13921 gacaataaga aaattgagtt tcacaagtat ttacctttcg cgatttattt atcattatca
    13981 ctgtcaaagg taaacaaatt cttaataaaa taaaaaagtc aatggattat tatgtgtatt
    14041 attatttgaa agcgacgccc gtgatctcat aagatttacc accgctgggc gtgctgacct
    14101 cgacggtatc gccttttttc ttgccaataa gcgcccgcgc aagaggtgct gtcaccgaca
    14161 aaagaccccg tttaatatcg gattcatcac tgccgacgat ttgatacgtt gcctcttctt
    14221 cggtgtcttc atcaataagc gtgactgtgg caccaaattt aatatctgtt ccagtgatcg
    14281 cggagacatt aataatctcc gcacggccaa gtttgtcctc cagctctgcg atgcgccctt
    14341 cgataaatcc ctgacgctcc tttgccgcat gatattcagc gttttcagaa agatccccgt
    14401 gctcgcgcgc ttctgcaata gctttgatca cagaaggtct ctcatgagac tttaagtgct
    14461 tgagctcctc ttgcaagcga tcaaaaccag atgatgtcat gggaattttt ttatccattg
    14521 tgccgcccaa aatcagtatg aaggtaaata tgttaaatcg agttcccatt cttccacaaa
    14581 agcagaaaag aattcaagat tttttgatca aaaggctctc aaaaacacga gaaccttttt
    14641 tgttagtgga ggccggagcc ggaatcgaac cgacgtccaa ggatttgcaa tcctctgcat
    14701 gaccattctg ccatccggcc agaaagcaat agcgtgcatg tgcattttct cgtatagtct
    14761 tataaaggat ttaagtcaag cttttcatca agatgtcttc tggtatcgat ttttatcaca
    14821 tgcgtcaaca catgatcaaa gcacagctcc aacctcagca tgtgattgat tcagtgattc
    14881 tcaaagcctt tgaaaaggtt ccccgcgaac tttttcttga acctaaaatg cattttcaaa
    14941 gctattatga taccgacatc gagatgtcga cagatcagcc caaaaggttt cttatttcgc
    15001 ctcgcaaact atcaaaactt ttcaatcttg ttgatttttc aacgtctcct aagattcttt
    15061 tgattggttt tgccacagga tacagccttg ctcttgcgta tgagatgggg gcgaaagtct
    15121 atggcataga ggaagataat ctcctttata gctttgccca aacagccctt caaaactatt
    15181 ttgatacgtt ttatggggtt actaattttg atgaagagct tcttattgat catcaccccc
    15241 tagcaggagg gctcctagag catagccttt atgattacat tatcatcgag ggaggtatcg
    15301 aaactcttcc ctcgacgctt tttgatcagc tgcgccctca gggaaaaatc atcggcattc
    15361 attgtcacca acatccgatg ggtgtttttt ctgccacaaa agagggaaaa atgcatatcg
    15421 atagctttga aagcgcttcg attctcaatg gttttcacac acaaaggaga tttgcactat
    15481 gatagttttt caacacacag aaattacttc tgaagaattg tttgatcttt tgcaaaaaaa
    15541 cagtgatgtt gtgcttattg atgtgcgcga agaagatgaa tttaatttgt gtaaaataaa
    15601 tggcagtatt aaccttccgt taagacattt ggtgcaaaac ttgcagaaga tagagcagga
    15661 aaaacctctt gtgacgatat gccatcatgg tgttcgaagt atgcaagcag cgattttgct
    15721 caaagaatat gggtttaata atgttgcaag tcttaaaggg ggacttgata actgggcaac
    15781 aacagttgat ttaacgatgc cacgctatta ggaaagaaca gataaaaatg acactcaaaa
    15841 aaatatccat ccttatgcta acaaccgcta taacctccgg tgcgtgttat gctgcagcag
    15901 ctccagcaaa gacggcaaca gcagcacctt ctgctaaaaa cacaacggtt gtgaaaaaaa
    15961 atgatacagg tgtcgatact attttttcgg ctcttgagtc tgcctatcaa aataacccag
    16021 agcttgcggc tgaaatcaca aaagtgaacc aagccgacga gggaatggtc caggcaaagg
    16081 ctggttaccg tccaaaagtc gatggtaccg taagcgtggg cgccaccaat acaattaatg
    16141 gcggcgagct taagagtatt ggctttaata ctatcgcgga acaacaaagt gtgcccacaa
    16201 catcagcggg catcaacgta tcacaaaatc tttatcaagg tggcgcaacg attgcctctg
    16261 taaagggcgc agagcaccgc atcaaaggcg aaagagcccg tcttttgatt ttagaacaaa
    16321 acatctttat tcaggttatt caggttatct tagatattat tctaaaagaa tctgaaattg
    16381 atctctatga aggaaattta aaattgctcc aacaaaccct tgatgcaacg aatgcaaaat
    16441 ttgctgttgg tgaagaaacc cgtacgtctg ttgcggaatc agaagctcag cttgctgatg
    16501 gcgcagccca acttgagaca gcacgttcag aacttgaggg gcttcgtgca acttttgcaa
    16561 aactcacagg gcgtcaagct aaaaaactca ttaagcctga tgtttcaaaa gatattccaa
    16621 aaactctcga gcaagcgatt caatttgcca cagaaaataa tccaacagtc attgctgcaa
    16681 aatttgatga agcttttgcg cgttatgagg ttgatcgcat cggtgctggg cttcttccct
    16741 caattgacct aaaagccagt agtactttta ctgcatctac ggatcgtacg cgcttttctg
    16801 ttagagatag gatcgttagt gggggagata caaatacaaa cctcaatgcc tctatcacca
    16861 tgacagtacc tctttatgag caaggggctg tacgttcaca aaaacgtagt gcgcacgaag
    16921 ctgctgcagg ggcgcgtatt tccattgaga cacagcgcag gcttgttgtc gaaaaacttg
    16981 tttcttcttg gcaaaactac ttagccgcac ggattaatat tgagaacttt aaaaaacaag
    17041 tccaagcaag cgaagttcgt ctcgatggca cacgtcagga aatgcaggtt ggaacacgcg
    17101 ttcttttgga tgtattaaac gcccaaagag atcttttaag ttcgcagctt aatcttgtcc
    17161 gcgctgaacg tggttacctt tttgaggcct ttaatgtgat tgcaactatg ggcggtttaa
    17221 cagctaaacg catgaagtta aaagttaatt actataatcc tggcctgcat tataatgaga
    17281 ctaagggaaa aatataaaaa gttttatagg ataaccatat gatgacaaat cgcaaagaaa
    17341 atgatcagga gatgtccatg gaagagatct tagcctccat tcgcaagtat gtcacagatg
    17401 atgacagacc taagtatcaa acctcaacaa tctcggatat agaggaggaa ccccttgatc
    17461 tgcgtcttgt tagccccccc caagaaacgg caacgcggcc agagccagca gcgtatacac
    17521 cgcccccacc tccgacacaa gagcattcag aacctcttga aacgcatccg ctatcctcac
    17581 ccgcaaacgc tcaaaaacca gcacaacagc ccatcaacgg tatcaatgga ttaacttccc
    17641 ctgaagcgat gactgcgtct gcgcacgcgt tatctcaact gagagagacg atacagtctg
    17701 cgcaaaagca agaagaaacg ccagcagctt cccctgcaat gcaaaataca tctttagaag
    17761 ctattgcgat gcaagccatg gggccctatc tcaaacaatg gcttgatacc aacctttctt
    17821 tggtcgtaga gcgccttgta gaaaaagaaa taaaacgaat tacagaggaa tttttgaaaa
    17881 aata
//
LOCUS       DAIAHG010000005        17026 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_5_length_17026_cov_3.436309, whole genome shotgun sequence.
ACCESSION   DAIAHG010000005 DAIAHG010000000
VERSION     DAIAHG010000005.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 17026)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17026)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17026
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5_length_17026_cov_3.436309"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4433 a         3574 c         3951 g         5068 t
ORIGIN      
        1 tagaatcatc ttataaagac gaaaaaaggt tagtaaaaat gtgtggtatt gttggattaa
       61 ttgcgcgtga cgatgttgtc aataaaattt tagaagggct cgaacgccta gaatatcgag
      121 gctatgactc tgcagggatt gcaacgattc atgagggtaa actcgatcgc cgccgctctg
      181 aggggaaact gcgtgcgctc aaagaagtgt gctttcacgc gccccttcaa gggaccattg
      241 ggattggtca tacacgttgg gcaacacatg gtccagctat cgaggcgaat gcgcatcccc
      301 atgccaatca tcgtattgct cttgtgcata atggtattat tgaaaatttt agtatcctaa
      361 aaaaagatct tgagacgcgc tatacctttg aaagtcaaac ggataccgag gttatcgttt
      421 atctcatcga ggacgagctt gccaaagatc aaaatccttt gaaagcgctt caagcggctg
      481 tgaaaaaact caaaggaacg tacgcgattg ctgtcctttt taaggagagt ccagacacac
      541 tttatgcagt gcgacaggga agccctctcg tgcttgggat ttcagaaaag gcgtcgtacc
      601 ttggatccga tgccatttct ctcgcaccgt gggtaaagca actttgttat ttagaagatg
      661 gagatttagc tgaactcaca ctcggtcatg tcactcttta taaccgtgaa gatcaaaaag
      721 ttcagcgtcc ttttgtcgcg aatccgatgg ccacggaaaa tattagtaaa gggccctatc
      781 gtcattatat gctcaaggaa attttcgagc agtcaaccgt tttaagtcaa gggcttcagc
      841 atattttaga aaaagaggcg catcatcttc acgctcctca aggtgacatt gattttaaag
      901 ctattgagtc tttatcgatt attgcctgtg gcacgtctta ttatgcggca atggtggcaa
      961 agtattggtt tgaaaaatat gcgcatattc ctgtggatgt ggacatcgcc tctgaatttc
     1021 gctatcgcca tcctgtgttt acaaaaaacc atgcgtgttt ttttatctct caatctggcg
     1081 agacactgga tacgcttgca gccctcgagc tttctaaaaa acatgccttg accttgggga
     1141 ttgtgaatgt gccgcaaagt tccattgcgc gggcggcgca ccatgttgtg tttacccatg
     1201 caggtcccga gattggtgtg gcttcaacaa aagcatttac gtgtcagctg ctcacacttt
     1261 tgacgcttgc actcgaggct gcacgtcaac gcaaatatct gagcgcgcgt gaagaagagt
     1321 cgatgatcca aagtttacga tcacttcccg gtcttgtgca tcaggtgttg atcaataata
     1381 aaatggaggc tgttgcacaa acaattttaa aggcgcctca tgtgattttt ttagggcgcg
     1441 gcacgcaata tcccatcgcc ttggagggcg cgctcaagct caaggaaatt agttatattc
     1501 catcacaagc gtatgcatcg ggagagctta agcacgggcc tattgcgctt attgatgagc
     1561 atgtggcgat ttttatcctt gctccttttg atgcgtggtt cgaaaagtca gcgggcaata
     1621 ttcaagaaat tcacgcgcgt aaagggcata tgactgtttt tacggatgag gagggggccg
     1681 agcatttaca aacgatgttg cccggcgttt tccaccttgg gaaaaatctt atccttcttc
     1741 ccaaggttga tcctcttctt cacccttttc tttatgtgct gccgcttcag cttttgtctt
     1801 attatacggg aatgctcaaa ggaacagatg tggatcaacc gcgcaatctt gctaaatctg
     1861 taacggtgga gtaggaaaaa aatcaaaaat ggataggaaa tgtgctcgct tttagaaggg
     1921 gagcattttt tctttctcag aaaaagagct ttaaatcttt attctgcttt agctcaaact
     1981 tcttacgctt ttggcatgtg taaaacaata acgaggcgtt gacagttttt ctttatccta
     2041 ttagcataaa atttaggaaa attttaataa tgtggctctg atgacagcac ctgtcaaaca
     2101 aaagcgacct ctttcgccac acctgcaagt ctataagcct caactgacaa gtatgctttc
     2161 tattcttcat cgccttaccg ggatggcgct gagccttggg gcttttctcc ttgtggggta
     2221 tctttattgt ttaacagaaa gcgaggcgac gcgggggtgt cttttctctg tgactcaagg
     2281 gccttttttt aaaattataa gttttttgtg gctctttgcg ctttgttttc atttattgaa
     2341 tggcgtgcgt caccttgcgt gggatatggg gtatggtctt gagataaagc aagtgtacat
     2401 ttctgggtat agtgttttaa tccttagcat tctttttacc ttttttcttt atggagatct
     2461 tttatggcgt taatcagttc agaaaaaggg cttacaccgt ggaaacatca gcggctcacg
     2521 gcggttattc ttttattcgc ggtggctttt tttatctatg cattctgtgt gacgattaaa
     2581 gcgtctcagg cggatattat cctttggctg caaaaaccac tcatcaaggg gttttgtctt
     2641 ttgtctcttg gagctctttt ctatcatagt tttttagggc ttaagatgat cgtggaagat
     2701 tatctgcatc acaaacatct acgccaggct gtgctttttc ttatgttaat tgcgcatgtt
     2761 ttttttacgc ttcttggcgg tcttttaatc ctctcttttt agttaaggca aatacagtat
     2821 gacacaagcc tataccacac acattcatga gtacgatgtt gttattgttg gtgcaggagg
     2881 atcggggctg cgtgctgccc ttggtatgtc cgcagccggt ttgaaaacag cgtgtgtgac
     2941 aaaagttttt cccacaagaa gtcacacagt tgcagctcaa ggtgggatta gtgcagcgct
     3001 caacaatatg aaagacggcg atgactggcg ctatcatttt tatgacactg taaaaggttc
     3061 agattggctt ggcgatcaag atgcgattga atacatgtgc cggaatgcca tcgaggctgt
     3121 ggtcgagcta gagcatatgg gggtaccttt ttcccgcacg gatgagggga aaatttatca
     3181 acgtcccttt ggggggcata caattgatca tggcaaaagt atggctaggc gcgcctgtgc
     3241 cgctgcggat cgcacaggtc atgcgatttt gcatacgctt tatcaacaaa gccttgccca
     3301 taatacccaa ttttttatcg aatatttcgc ccttgacctt attatggaag acggtgagtg
     3361 ccgaggtatc atcgcttggt gtttggatga tggttctctt catattttta gagcgcatac
     3421 cgtagttctc gctacagggg gctatggccg tacttttttc tcgtgtacag gtgcgcatac
     3481 atgtacgggg gatggcaatg ccatggtact gcgcgcgggg cttcctttgc aggatatgga
     3541 gtttattcaa attcacccca caggtattta tggtgcaggc tgtcttattt cagaaggtgc
     3601 gcgcggcgag ggcggatatc ttacgaattc taaaggcgag cgttttatgg agaaatatgc
     3661 gcccaatacg aaggatttag cctcccgcga tgttgtcagc cgtgctattg ctgtggaggt
     3721 tttagagggg cgcggctgtg gccctgaaaa ggattatgtg catttgcatc ttgagcacct
     3781 cagtccacaa gttctgcacg agcgtctgcc ggggatttct gaaacagcaa aaatatttgc
     3841 gggtgtggag gtgacaaaag aaccaattcc tgttgtccct actgtgcatt acaatatggg
     3901 gggcattcct acaaatattc acggtgaagt tgttaaaaat ggtcaaaaag aaacggtttc
     3961 ggggcttatg gctttgggtg aggcagcctg tgtttctgtg catggtgcca accgtttggg
     4021 gacgaattct ttgctggatc ttgttgtttt cggtcgtgca gcagcccttc gtgcggcaga
     4081 aaccataaaa cctggaacac ctcataaaac aatttcaccg gatctttatg cgcatacact
     4141 tgcgcagttt gaccagcttc gctatgccca cggcgatttt cccacagggg agattcgtgt
     4201 cgcgatgcaa aaaacaatgc aaaagcattg tggtgttttc cgtaatgatg cactgcttaa
     4261 agaggggctc gacgagctta aaaaagtgca agaatctatg aaaaatattc gtattaatga
     4321 tcactcgctg atttggaata cggacttgct cgaggcatgg gagcttaaaa atctcatggc
     4381 acaatgttgg gtcactcttg agtctgcatt gcaacgccat gaaagccgtg gggggcatgc
     4441 gcgcgatgat tatcctgagc gcgatgataa aaattggctc catcacaccc tatcttccct
     4501 tgatggaaag aacaaagtga cttttgacaa acgccctgtc tctcttacac cgctgacaaa
     4561 ggacgtggag ccgattccgc ttcaaaagcg cgtgtattag gaaaaaaggg ggagagcctc
     4621 caccctctcc catctgtatt ttactcaata tacatatcga tgaatttttt gtttttttga
     4681 agattataaa tttcttcctt tgtactcgtt gtgataggct tcattttgta ataaagttct
     4741 aagagataat ctaatccgtt tgatgtgaaa gcaatgaagt gggttacgta ttgtgtgtcg
     4801 aaaagggagt accctgaaag attatttgga tcagtttgaa aatttccttt attacgtggc
     4861 gttaatccct ttaaaaaaga tttgctgatt ttatcctcaa gaagattttt ttttctatta
     4921 ctgcggcatg tcagtgagtc aagctcccat ttattgcctt tgctatcttc aacgtatcct
     4981 tttcgatgtt gaagagcatc gatgatatcc tctaatttga acataggatt ttttattttt
     5041 tcctgccaag atttgacctt taaaattgta tttgcattaa cctctcctgc aaggggatag
     5101 aacatgtacg ttccgttaat aattccattg tcgtcagtga tatcaatagc gcatccaata
     5161 atatcataag gtgcatactc taaatgtcta tattcgtctg ctttggataa acgaaggttt
     5221 gtttcgtctt caatgggcat ttctcgtaat acatccacaa aaaagttttg aaactttcca
     5281 agcccttcga ttattttcca ttgctttgtg gttgtatatt ttgtgagctt tttcttaagg
     5341 tcttgacaga ttttttcccc ttgttctgtg gagctgacgt tgcttataat ctcagaagcg
     5401 catgcccccc ccccagtata tggtgagtgt gagagtaatt aaagattgaa tgattttttt
     5461 catgatagaa atttccatat taaaaataat actaacgata agtaacatat catgaaaaac
     5521 ttaagatttt cctaaggctt tttcgaggcc gattattctt ccattgccgt tgtgtcgggt
     5581 tctctgatat aaataaattt agctattttt aaggcgtgtg gatgtgatga ctgaaatgaa
     5641 cggtgaggcg tctctttttt ttaaaaggtt tttgagaaac cctgcgaggc ttggcacact
     5701 tgcacctgtt tcagaacgtt tggcgcgcct tgcggcgtca tgtgtatcac ttccctctga
     5761 cggcaaaatt gtcgaaattg gtgcgggagc ggggcgcctc acgcgtgctt ttttaggggc
     5821 cggggtcaaa ccaaaaaacc tcaaagcgat tgagcttgat ccggatcttt gcgcattcgc
     5881 aaaagaaaat ataaaaagcg tagattttat cgagggcaat gcaaaggatc tcgcaacgct
     5941 tcttccaaaa tcttggattg gtagtgttga tattgttttt tcaagcatcc ctttcatgta
     6001 tttacctcgg gaaatgcgcg cagagattac agaatctgtt tttgatgtgc tgaagcctgg
     6061 tggggatttt ttccacctga cctataacta ttggtcgcct ctcgagggga tggggtatga
     6121 acaaaggcgt tgcgcaacac tttgggcgaa tttccctcct ggctttattt ggcgatatcg
     6181 caagcagatg atacctcact tgagagctgc ttaaaaaatg ccaaagactg ttgaaaaaaa
     6241 tattaagcgt gaggaaaact ttctctttct aagacagctt gtgaaaaatc ctaaaatgct
     6301 tggggcgatt gtgcccagct ccgaggcgct tggaaaattt atctctaagc aaattgtcat
     6361 tacagaccaa acacagtgtg ttgaaattgg tgcagggacg ggcagtttga cactcgctct
     6421 tttgcgtgcg gggattcttc ctgaaaatct ttatgttgtg gagcttgata caactctttc
     6481 ggatttttta aaaaagaaac tacctgccga agttcatgtt attaaaggtg atgcggcgca
     6541 tctcgagaaa cttcttccct cgcatattat agggcacgtg gatacggttg tgtcaggtat
     6601 tcccatgatg aatttatcga aggcagagca aacggccctt attgagtcaa gttttaatgt
     6661 gatgaagaat gagggaagct tactacagtt tacatatggt cctctttcgc cgctgcccgc
     6721 aaagaaatta gggctttcta aaaaacgcat cggacatgtt cttcttaatt tcccaccggc
     6781 aacgatttgg cgttatcagc gtagctctat cgcgcaataa ataagaagcg tgtgaggggg
     6841 gtacgttttg ttttgcaatg ttattcttcc gcttttattt gatcgcgcgc ttacatatca
     6901 aacagatgaa gatatcgggg taggaactct ggttaacatc ccttggcgca ataaaactct
     6961 ttttggtctt gtgatagaaa cggacgttgt gcctctcgag aatgtgacga tcaaaaaagc
     7021 aatccctctc cccttgaatc ttgagcgaaa gatgttggct tttatgcaac aggtagctga
     7081 atataatctt atgcctatag gatgggtgtg gcgcatggtt ttaggaggag tcaaccttga
     7141 taaaattatt gatagtgatc tgacactcaa aacacctcat ttatccttca ttgacgcgcg
     7201 gacaaggcat cttaaaaacc ttaatgcgga tcagcagcag gctgttgatt ttatccatac
     7261 acaactcgat aaaggagcct atgcctcaat ccttttggac ggtgtgacgg gctctgggaa
     7321 gacagaagtg tattttcata gcattgcaaa aacgcttgat gcgcaaaaac aatgtttgat
     7381 cctgttgcct gaaattggtt tgtcctatca gtggctccag cgatttgaag cacagtttgg
     7441 ttttacaccg gtggtgtgga attcgcacac cacgcccaaa aaacgtaggg aggcgtgggc
     7501 aaaggctata agcggaaccc cgcaagtttt tgtaggtgca cgctctgctc tttttctccc
     7561 ttataaaaat cttggtctta tcattgtgga tgaggagcac gatagttctt ataaacaaga
     7621 ggagcaaggt cattatcacg cgcgggatat ggcgatttta aaggcttatc acgctgcaat
     7681 tccgattatt ctcgcttcag cgactccctc gcttgaatct tttatcaatg cagagcaggg
     7741 aaagtcaacc catcttcgcc ttaaaaatcg ctttggtgag gcccttttgc caaaggtcta
     7801 tttaatcgat cagaaaaata aaaatcctcg tttgtcaaaa ggatggctca gtgaggcgct
     7861 taaaattgct ctttacaaac gattaaaagc caacgagcaa tcgctgcttt ttctcaatcg
     7921 acgggggtat gcgcccatta ccttgtgtgc atcgtgtggg tatgtgtacg agtgtccggg
     7981 gtgttctgct tctttggtgc aacatcgtcg caaaggttat gcgcatcttc agtgtcatca
     8041 ttgtggattt gaaaaaaaac tggatcttca gtgccctcaa tgtcttacag ctgatacgtt
     8101 gattccttgt ggtcctggca ttgatcgtgt ttttgaggat gtgacaaagc tttttcccga
     8161 tgcacgcgtc agctctctta cgagtgatat gacgcccaaa gatcagcgtg tgctttggga
     8221 gcgcatgatg gcgaacgagg tggatattat tattggaacg caaatgattg caaaggggca
     8281 ccacttgccg catctgacgc ttgtgggagt gattgatggc gatatggggc tttatggtgg
     8341 ggatctgcgc ggtgaggaaa aagcttttca gcttttgcaa caggtcagtg gaagggcagg
     8401 gcgcgaggct aaacctggcg aggtttttat tcaaacctat caacctgaaa atgctctttt
     8461 caaagcccta caacagcatg atcgtgatca ttttatgcat caagaatcga cactgcgcaa
     8521 agagatggcg ctgcctccgc atacgcgttt aatcagtctt gtcgtgtcgg gaatggacga
     8581 gcacaaagtt aaggaatgtg caaaaaaaat agcactcttt ttaacgaaaa cacccgcgat
     8641 tcaagttctt gggcccgcac ccgcgcttat gctgcgcctc aaaagtcgtt atcgttatcg
     8701 cattttgctt aaagggggca aacaccttaa aatgagtgcc tggctaaaac aaaggctgtc
     8761 ggagctaaaa attccaacat cgatcacgct gactgtggat gttgatccaa tacagtttat
     8821 ttaaacaact gttttatttt ttttggatta tgtaattttt tattctcttt tttcttttct
     8881 gaaagttctt tgacatactc tatccatgtt atttcttcat tttcattttt ctttattttc
     8941 tctttggtgt tatttttttg agtattttct tttttgtgct caatcccatt ctcattctta
     9001 ttctcttttt cagaggcata aagagcagaa aaacaggttg ccatcaggag aagaaaaaaa
     9061 tagcgcattg ttataaaatc cttttggtga tgattaagca aacagtgtga tgggattttc
     9121 tataaaactt ttgaaggatg caaggaattt cgcaccaagc gcaccatcaa ccgcacggtg
     9181 atccacagaa agagtgcagt tcatgagcgt ggcttgggta accgtgccat ttttgatcac
     9241 aggcgttgga acgccagcac ctacggctaa aatgcaggcc tgaggcggat taataatcgc
     9301 ctgaaattgt gtgatgccat acatgccaag gttcgagata caaaaactcc ctccttgaaa
     9361 ctcatggggc tgcagcttgc cgtcacgggc acgctctgcc agctgtttca ttgtggcgga
     9421 gagattccca agggactgtg aggacgcgtc tcggataatc ggcgtgacaa ggcccccatc
     9481 aatcgccaca gctacagaaa tatccgtttg agaatagcga cggataaacg tctcatgaaa
     9541 agacacatta gcatcgggaa cttctttgag cgcttttgca gccgccataa tcacaaaatc
     9601 attgacggag attttattat gtttctcgcg tttttcatta aggcttgtgc gtatttttaa
     9661 aagttcatca atctcgcatg tgatcgataa ataaaaatga ggaacggttt gtttcgattc
     9721 cacaaggcgc ttagcaatcg ttttgcgcat catggttagg ggaatatcgg tgaagtcact
     9781 ctcttttgac gcggcgatgt gtcctgctga gggtttgtgg gctgttgcac cctccacgtc
     9841 cgccttgata atacgtcctc tgggccctgt tcccacaaca gtggacagat ccatattcaa
     9901 agaagacgca aggcgctttg ccacaggtga ggcaatcaca cgattattat ttgcagaagg
     9961 cgccgtagca ggtgtgaccg caaggggtgg tgtattgtct tgtaaagacg gtgtaggcgc
    10021 agcgtgcgtt gtcgtgacgg gcgatggttc aactttaata ttctccaaag ccgatgcgtc
    10081 ttcaccttcc tcaaggatta gtgcaatgag tgtattaact tttactgcct cagatccttc
    10141 tggaataagg attttaccaa gtgtgccttc atccacggct tctacttcca tggtggcttt
    10201 gtctgtctca atttctgcaa ggacttggcc tgctttaata ctgtcgccct cttttttgag
    10261 ccatttgaca agatttcctt ctgtcatggt ggggctaagg gctggcatta aaacctgaac
    10321 gggcataagt atctcttcct gagttcgtat gttttctcta tcaaaaagaa aacatgtgct
    10381 gagtatgtga tcaaaagtct accaaggcct cttttatcac gcaaagcttt tttaaaaaag
    10441 cttttttact gaagggcgct cttggagtag agtgaaggga ataaagatta aaaaaatata
    10501 aaaacatgtt gtatacctcc caaactcttg aacagcttga gcacattgca gaagattttg
    10561 ctaaaacact gataccacct tgctgccttt tactctacgg aaatttaggg gcagggaaaa
    10621 caacctttgc gcgtatgctc attcaaagtc ttacatccaa ggataccgtt gttccaagtc
    10681 cgactttcac actcttgcaa acctatgaca tttcaaaaaa tcattccgtc caaggacaca
    10741 ttacgcactt tgatttgtac cgccttcacg ccgaggatga tttgttggat attggctttg
    10801 aggagtatag tcacgaacat atttgtctga tcgagtggcc agagcgtttg ggcgcgtttt
    10861 taccagaaaa atatataaga attatgctgg aggaggattc cccaacgacg cgtcatctaa
    10921 ccattgagtc cataggattt gagtgatggg acactcgcaa caattgctgt gtgagtcagg
    10981 agagctctat gattttctgc acaaaaacgg tctcgcctcc gcccccctag aggcgcttcc
    11041 tatggatgca tcaaagcgtc attattaccg tgtgcagggg gaaaataagc ttgttatggt
    11101 ctgtccaccc tgtcaaaaac cagagcaatt tatcaacgtt tcagcatttt tgaagaccat
    11161 tggcctcaac gcaccatgtg tccttgcgaa ggaaaatgat ttttacctga ttgaggattt
    11221 tggtcaaaat acctaccgca aggcgatggg aaaaacgccc ccaaaagaac tttatacact
    11281 ggcaacagat gtgcttattc atctcaaaga gcgcgtgcgc gaaaaaccgt cctttgtgcc
    11341 tgtgtatgac atgcaaatgt ttttgaatga ggctgcaatt tttcccgaat ggtacaaggg
    11401 gatacccgcc cacttgccat gctatttggg catttggaag gatcttttaa gcacactgga
    11461 ggtgccagag tgtgtgattc taagggatta tcacattgat aatttgttct atttacccgc
    11521 gtttcagggg ctcaaagcct gtggtctgat tgactttcaa gatgctgttt ttggccctgt
    11581 gggctatgat cttgtgtcgc ttctttttga tgcgcgtacg gatgtgccac aaaatttggt
    11641 tgattttctg atggatcgct atatcacaac gtttcctgcg gcaaagcaaa gtgctcttga
    11701 aagggcctgt acaattctca atatgcacag gcaactgcgc attcttggcg ttttttcaag
    11761 gctgagtcag cgtgatggga agcatcacta tcttcagtat gtaccgcgta ttttaaccta
    11821 tgttaggacg cttttcaaaa atcccctttt caaggatttg aagcgctggt gcgacgccaa
    11881 agggggattt taatcaaggg ggcttttgat gtttacatag ctttgagcct tttcatgact
    11941 tcctctttgc caaggatgtc catgatttca aaaatactcg gagaaatagt gcggcctgta
    12001 agggcaatgc gcacactttg agccatatca cccagtttca cagaccagag ctcggcatgc
    12061 gtgcgaaaaa gtgtttctaa ggaagagctc gaaaagtcat gggtctcctc aagcgctgcg
    12121 ataattttga ctaagagggt tttaagggcc tcattaaaaa gctttgtggc cttctcatcc
    12181 aggggaagac gcgtgatata caactgtgcc atatcggcaa gctcaagaag tgttttggcg
    12241 cgttgcttaa gtcccgccat gcctttttcc aggcgatgtt gatcaaggtc cgtaagcgta
    12301 tatccttttt tctcaagaaa aggggtgagc tcagaaagca gcgcaaggtc agccatctcg
    12361 cgcatataat ggccattaag gcttgtgagc tttacaatat caaaacgtga gggcgacttg
    12421 ccaataccgc tcacctcaaa aagtgcaatc gcgcggttgg tctcaataat ttcctcatcc
    12481 ccatgtcccc atcccagacg cagcaaataa ttgcgcatgg cctcggctaa aaaacccatc
    12541 tctttgtatt gttcagtgcc aagagcgcca tggcgtttgg aaagtttagc accatcggga
    12601 ccatgaatca ggggaatatg cgcaaaagca ggcgtgctcc agccgcaggc ttcataaata
    12661 tgtttttggc gaaaggtgtt ggtcaggtga tcatcgccgc gaatcacgtg ggtaatgccc
    12721 atatcgtggt cgtccaccac aacggacagc atataggtgg gcgtcccgtc cgaacgcagc
    12781 aaaatcatat catccagctg gtcattcgca acctcaatat gaccttgcac aaggtcatcc
    12841 acaatggttt ttccttgctg cggtgctttc aacctcaggg cgggcttgac acctgtaggc
    12901 gcttccctcg gatcgcgatc gcgccaaagg ccattatagc ggggattttt tccctcacgc
    12961 tttgctgcgt cccgcatggc ctcgagctct tcgggcgtgc aataacaata gtaggctttg
    13021 ccttggtcta aaagggtttg cgcaacctct tgatggcggg cggctctggc ggattggaat
    13081 acaacgtcac cgtcaaaagt aaggccgagc cagttgagtg attcgaaaat tgcctcaacg
    13141 gcctcttgcg ttgagcgctc tcggtccgtg tcctcgatgc gcaaatgaaa tgttccccca
    13201 aaatgcttgg caaacagcca attaaagagg gctgtgcgcg cactgccaat gtggagataa
    13261 ccggtgggag agggcgcaaa acgggtaacg acagagggca tagatttttt ggatgtgaca
    13321 ttttccccca ctatatcaaa aatttagcag gtgtggctat tgttttgcgc ataaaaaaac
    13381 caccccgaag ggtggctttg agtcaagata gaaaaaatct tagatagcac taatgtcgtc
    13441 ttccttacca tctgtaaggt ctaataattt gttgttatat gagtttttga gtttatttaa
    13501 tttaatcttc ttatcatcct tgtcgagagt cttatcctta ttgactgact taacttcatt
    13561 atcatacttt tctttgagct cctcttttgc ttccttaagt gcggtacctt tcagatgctc
    13621 tccgacctct ttttctgcgg attcgaaagc ctcatcgatg ctttcggcaa aatctttatc
    13681 atcttgataa gcgtctttga gggagtttac acgattcaca agcaaattgt agagtttgtt
    13741 tgattgctct tccagttttt tattgttagg gttaaaatca aagttgttac ttttggtgtc
    13801 tacaaacata ccgttaagct tgttgccagt aacaacggtg tggataaggt cagtcacggc
    13861 attctcatga ccatcaatat caaacagctt gtccaaaagc ttctcgagtt ttacagaggc
    13921 ttttgcctca ccttcattca atttataaga ttttttctcc ttattctcat tttcaatatt
    13981 gagtttttta tttgaaaatt cgccaagccc aaaatgttta tctgctgcat ttgctttggg
    14041 agctccaacg gcaacgatga gggctgctgc ggaaagtaat agtgataatt ttttcataat
    14101 tcacaccttt aaaaataatt gttaaccaca ttatgacgat gctatcgtca ccttacataa
    14161 aaatcgtatc atgtcaaaat actatatgca agagcttttt tttaaaaaat gtattttttt
    14221 ttagttaaat acatcaaaat aaaagtctcc aatactaccc taaactacaa aggttaataa
    14281 ttcgtaaaaa attaagagat atttgactta ttttttatat ttttaccttt gccatccgcg
    14341 cttcttttga ctacattgga acttaaatac gatttcctat gaaaagacct tacacggtta
    14401 tgaatacctt ttgtcaacgc tttgtatttt tgtcgagtgt tttttttctt gcagcgtgtc
    14461 aagaaagtaa cgatgagtat gtggaacgtc ctgtggagga gttatacaca gaggcgatga
    14521 atcggctcaa cagtggcagt ttttcaaagg cagccgaggg ttttgcagag gttgagcgtc
    14581 agcaccccta ttcaaattgg gccttgcggg cacagcttat gtcgggcttt gcgtactatc
    14641 aagcaaaaaa atacgacgag gcgatcgaga gcttttctgt gttcataggc cttcatccta
    14701 tgcataaaga tgtgccctat gcgcaatata tgatcggtat ttgctattat gaacaggtgc
    14761 cgattacaga gcgtgatcaa cgttctacag aaaaagcact tctggccttt aaagaagttg
    14821 tggatcgcta tccaaacagt ccttatgcaa aagatgcaaa gattaaaatg gattttattc
    14881 acgatcattt ggcggggaaa gaaatggatg tgggacgcta ttatcagtct cagggaaatt
    14941 atttagcggc tgtgaatcgc tttaaaattg ttgtggctca ataccaaaca acaaaccatg
    15001 cccaagaggc gcttcaccgt ttggttgagt gctatttaag tcttggttta gtggatcaag
    15061 ccaaggctgc cgcggcggtc ttagggcata actatcccgg aagcccctgg taccaagagt
    15121 cttataatct cctcaatggg aaaaagaaag atcactaacc tatgctttca cgcctttata
    15181 tttccaatct tgtgctgatt gataaactag aggtggtttt tgaaaaaggg ttgaccgttt
    15241 tcacaggcga gacaggggct gggaaatcca tgctgttgga tgcactgggc cttattcttg
    15301 gggagcgttt tgagagccat cttttgcgtc ccgggcaaca gtctctttcc attacggcat
    15361 gctttgaggc gacaaatcat atgtctgctt tccttgacaa tcagggcatt gatcaagaga
    15421 tttatgatga tgatcttttg ttgaagcgca ccgtggatat caaaggggag gatggcaaaa
    15481 gcaaaggccg tgcctttatt cacaatgaac ctgtgacact caatgtgctc aaacaatgtg
    15541 ctgaaaaaat gatggatatt cacggtcaat tcgatcgctt tatggatcct tcttattata
    15601 ttgatcttgt tgatgctttt ggggggcatg cgacccagtg cacagcagtt aaaaaaacct
    15661 atcatgtatg gcgcgaggct gaggaggcgt tgaatcagct gcactttaaa caaagtcatg
    15721 ccaaagaacg tcacgagcaa ttgcaatttg atatcgagga acttcgtcag ctcaaccctc
    15781 aaaaaaacga ggaagatctt ttacttagcg cgcgcgaggc ccttaaaaat gcatcgattg
    15841 ccggggaatc ttttcaaaaa gcggcaactt ttttagccgg tcaaggcgat caaggccttg
    15901 cttttccttt gattcaaatt caaaaacttc tttcgcggtg tgagggagag aacgcgacca
    15961 ttacgcgctt agagcaaatg attgtagatc ttcacgatat tgaatcggag attcatgcaa
    16021 aggccaccgc gcttcctttt gatcccacgg cgtttgaaaa aagcgaggaa aggctccata
    16081 accttcgcca gcttgcgcgc aaacataaaa caaatgcgaa tgatttagcg gatttgatgc
    16141 aaagttttca ggaggaatat gcgtcgcttg agcataggga ggaggcactt gccgcgtgcg
    16201 ctgcaaatct aaaaatgtgc gagcaagaat ttgttatgca tgcaacgcac cttcatcaaa
    16261 accgtgtcaa ggcggcaaac acattatcag ccgccattat gaaggaattg gcgccgctta
    16321 aactcgagca cgctcttttt aatgttgcaa tcgcacctct ttcaattaag gaagcccatg
    16381 aaaaagggat ggaccgtgtg gattttcttt tttcagccaa ccctgggata gagccacagt
    16441 ctattggaaa agtcgcctca gggggtgagc tttcaaggct tatgttagcg ttgaaggtgg
    16501 tgctttctca agcaggtcac gtgcaaacac aaatttttga tgagatcgat agtggggttg
    16561 gcggtgcaac agccacagcg attggccttc gtctcaagcg tctttccaag aatcagcagg
    16621 tgattgccgt gacgcattca ccccaagttg cagcctttgc ggatcatcat tttaaggtta
    16681 ttaagcatgt gaaggaagat caaacgtttg tgactcttga aaaacttcag cacaaagagc
    16741 gtgatcatga gctttcgcgg atgctcgctg gagatacagt tacgcctgag gcgcttcaag
    16801 ccgctcggtc ccttcttgag caggcggcta cgcaagtgga ccgttcagcg tttgagcagc
    16861 ctatattgtc ctagacaaag agcaagtgat caaaaagagc actcgcccct agcattctcg
    16921 ttttagacga agctgtctaa ttttctcgct ctttttattt taatttaatt caattcaatt
    16981 cacctgttcc tggcgcaata tcactgggct cttctgggga tgtctc
//
LOCUS       DAIAHG010000006        14983 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_6_length_14983_cov_3.731987, whole genome shotgun sequence.
ACCESSION   DAIAHG010000006 DAIAHG010000000
VERSION     DAIAHG010000006.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 14983)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14983)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14983
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6_length_14983_cov_3.731987"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4384 a         3288 c         2942 g         4369 t
ORIGIN      
        1 cccgagccgg actcgaacca gcgacctagt ggttaacagc cacgtgctct accaactgag
       61 ctatcgggga ataagataag cattttatag catgcactta acaccctgta aagaggaata
      121 ttcctcgcta aacaaaaaag agagcccaca aagtggctct ccaaagtttc tataaaatat
      181 ttatctcatt aatttttttc aatcgtttct tgttttttgg tttcttcatc atcttctcca
      241 aaattttctt tgtagttttt ttctccgaag atctttttat acttttcttt tagcgttttg
      301 cggtcgttaa ttcttttatc cagagtttct tgatacatct tacaaaactc tccggagtca
      361 ttctttattg cgctctttaa atcttgtata tctttttttg tgtttaaaag aagataaata
      421 atttgagttt cttccttttt ctttgtctca taaatagatg taatgttttg tatgattttt
      481 gaattaggat caagaagctc aaaatcaatt tctttgtttc gaagatatga cattccttgt
      541 tttgtctcat tggaaggatg ttcagtcgtt attcctctgt tgcaagggat gctctagcaa
      601 agaggcttgt gagagcaagc gtcttaaaat tttattaaag gtaattataa tctccatttt
      661 tattgtgtta gtgctttgta gcaccgtggt tttatatcag aaaaaagaga gaatacaact
      721 ataattttaa ttatcgtcgt gtttatgatc atgatggtga tgataaggtg cgatggttga
      781 cggaacagcc cccccaaaaa gccccaaata ttcgggatgc ttgcgcacat cttcggggtg
      841 tccttcacag caaatatgat gattgagaca aaaaaccttg tcactcgttg ccatcaccat
      901 gtgaagatca tgagacacca aaagaacggc acagtttgtc tgacgccgcg cagcatcaat
      961 ataaggataa agctcgtgtt gagatgtaat gtccataccc tgagagggtt catctaaaat
     1021 aagcacatcg ggcgcctcaa gggttgcttg aacaagaagt attttttgaa gctctccccc
     1081 tgacaacgag gccattgcaa aagacgcttt gtcgagcatt tttaaagaat cgagtttttc
     1141 ttcgatttta gcgatatttt ttgtaaataa agtgagaaaa tccatgacac gcagaggtaa
     1201 aaaggtatta aacactattt tctggggcac ataccctatt tttatccctt ttttgcgttt
     1261 aatcgcgcct gatgtcggtt cttttaatcc taaaactaac tttaaaaggg ttgtttttcc
     1321 cgcaccgttc ggcccaataa gcgtcgtaat ctgcccttta aaaagctcaa gactcacatc
     1381 ataaagaatg cgtcgttctt taccctccgc cgtttgacgt gaaaaaccaa gttgatggat
     1441 agaaataagg ggttttgaca caggctcgcg tgctttcgat gcaaaaaaat tttcactaag
     1501 accccatcat accacgtttg cccccaatcc cactccattt ttcataaaaa atctgattca
     1561 aatcttgaaa aaattaactc tttattaact tttctctaaa gaagatgagg ggagagaaaa
     1621 cacttatgga cagatacatc atgcctcgat tcttcatctt tttactcctc caaatcctga
     1681 taacctcgca tcttttggcg agtaatgagg atgaaattct ctttaataaa gaggattctc
     1741 ttacaaagaa aaaacaaaaa atggaaaaaa tcataacgcc aagcattaaa gaggaggaac
     1801 ttgctcgctt taccgtcaca aaagggcgta tagactattg caaagaaaag gaagagaacc
     1861 tctattttca tattgagtat tttctgaagc accccgaagt ttttattaaa tttcctcaaa
     1921 aaataacgac tttttctctt tcgaaagtga attttgatga ggggaagaat ctagttccct
     1981 atagtcctaa ggtgttagaa agaatttctt tttacaatgg cattgattat ctcatacaaa
     2041 actgtacgct ctacaaaaat aatggcgatt ttttgattca atttgatgat gatgaaatcg
     2101 atatcctttc tgatcgtttg aaggaaaaaa atgtcgacac gttgtgggtt cagttttgtc
     2161 atttttcagg ggattgttca aaaaatttag caaagattct taatacaaat tcatcacttc
     2221 acacgcttca tatattctat aatgacacgt ttaatgacga tgccatggag tccctctatc
     2281 catctctcat aaaaacgtcc ctaaagcgct tgttcattag aggaaataag ctgactgata
     2341 aaagtgcaag aacaatcgct cagatagtca aatccctgga tcttgaagag cttaccatag
     2401 atgaatatgg tctgagccca aaagacgcta cgattatttt agaggcatta caagacagca
     2461 cgtccctcat tgaattttgt gttagcgcct atagagaaga ggatgagtgc gatttctttt
     2521 acacctctct ttacagaaaa atagaagcgc gcgttgatca gaatagaaaa aatcttgtga
     2581 aaactgttca agaagagtgg ggggtgaatg ataataaaag aaaagatgaa gatataacgg
     2641 aaatagtaaa agaggacaac ggttagaggg ggcctaagag cggaaccccc ttggctgctg
     2701 cgggaaagct aatttttctc tatgaccgac tagtcgtgta aaactctcta aagtggggcc
     2761 gagggatatc ccccagcctt ctcttttttt aggcttttgt ggttgctttt aacgttgtcc
     2821 ttgtgagaat aacagaaacg acgaggtaat ttgtctcacc aaggttacat tcctttataa
     2881 ggtcattaaa tgtttttttg cgaagatttt ttgttgttat ttcttgtgtg tattgatttt
     2941 tttctttatc aaagagcgct tggatttttt ttatcctgat gttagaaaaa ttcttagagc
     3001 caaacaaggc atacatactt tcgttcattt cctctttaag aaatgggatt attttttcct
     3061 gattaaaaca ctctaatcca gttttaaaat aagtatttgc aaaaaaatca gagtaattgt
     3121 ttagagagat gcgctgagaa gaatttgttt ttttatggcc tttttccgaa aatttgatta
     3181 gaaacaagaa atctttaccc ttaggattct ttccctcggc aaagtaagga ttatacagat
     3241 ctcctttgtc ttgtatacat tgaacaattt tgcttttact ttcctcctca atctcactcc
     3301 caagggcgga agtgatgagg ctgccttgtg ccaaacaaag agagatggca atatattgca
     3361 tgaagttttt cattgtatta acttttttat tgagttgata gggtttttat aagttaaaaa
     3421 taaattcctt acaataaaaa agcttctctt aatcatctcc catcttaagg gctgcgataa
     3481 aggcactttg gggaatctct acagagccaa actgacgcat acgtttttta ccttcttttt
     3541 gcttgtccaa aagtttgcgc ttacgggaaa tatcgccgcc atagcatttt gccgtcacat
     3601 ccttgcgaat ggccgaaact gtttcacggg cgataacttt gccgccaata gctgcctgaa
     3661 tcgcaatttt aaaaagttgg cgcggaataa gatcttttag tcttgagcat aattgtcgcc
     3721 cacgggtttc tgcacggctt ttgtaaacga tcatagaaag cgcatccaca acatctccat
     3781 tcacaagaat agaaaccttg acaaggtctc cctcgcggta ctcatccact tgataatcaa
     3841 aactcgcata cccacgacta atggatttaa ggcgatcata aaaatcaaac acaacctcat
     3901 tcagaggaag tttgtaaacg gccatagcgc gattgcctgc atacgtaagg tcaacctgct
     3961 cgccgcggcg ttctgaacaa agggttagaa tagagccaag gtactcgtca ggcaccataa
     4021 tggttgcacg aatccaaggt tcctccataa aatcaacctt catcacatcg ggcatatccg
     4081 atggattatg aagctccatc accgtgccgt tcgtcatatg aagtttataa acaacactgg
     4141 gtgctgtggt aataagatca aggtcaaact cgcgctcgag gcgttcttga ataatttcaa
     4201 ggtgcaaaag ccctaaaaac ccacagcgaa aaccatagcc aagagccgcc gaactctctg
     4261 gttcaaaatg aaaactcgcg tcattgagtt gaagtttttg caaactctca cgcaattttt
     4321 caaaatcccc cgcatccaca ggaaacagtc cacaaaaaac aacaggcaca gaaggtttaa
     4381 aacctggaag cgcctcggca cacggtttgc gttcttctgt aatcgtgtca cccactttgc
     4441 aatctgccac ggccttgatg ctggcggtaa taaaaccaat ttcgcccgga taaagagtat
     4501 cgaccatcac ttgtttaggt gcaaagacac cgacgcgatc gacctcgtac gtagcccctg
     4561 tacccatcat acggatttta gcgccttttt taaggacacc atctttaatc cgcacaagaa
     4621 tcatcacccc tagataagga tcataccaac tgtctacgag catagcttta agatgggcat
     4681 tcacatcgcc tgtgggggct ggaagacgtg ttacaatcgc ctctaaaaca tccccaatgc
     4741 ctatccctgt tttcgcagag atcatcacag cctctgaggc atcgatccca atcacatctt
     4801 caatttgcgc tctgacacgc tccggctcag ccgcagggag atcaatttta ttcaaaatag
     4861 gtacaatctc gtgattcgca tcaatagcct gatacacatt cgcaagggtt tgtgcttcta
     4921 caccttggga cgcatcaacc acaagcaaag atccctcaca ggctgcaagg gaacgactca
     4981 cttcataggc aaagtccaca tgtccaggtg tatccataag gttcagaata taaacctcgc
     5041 cgtcctttgc tttgtaattt agacgtactg tttgagcttt gatcgtaatg ccgcgctcgc
     5101 gctcaatatc catagagtca agcatttgtt ctttcatatc gcgtagttcc acggcgccac
     5161 acacctggat aagacgatcg gcaagggttg atttgccatg atcaatatgc gcgacaatcg
     5221 aaaagttacg aatttgagta agaggtgtca tggtcatgcg cgtaaggtcc caccggtttc
     5281 tttttcaatt aaagtaattg ttgttgtcat aatttgctca atctcaacct cactcagtgt
     5341 ggcgtgttcg ggctgaaggc gaaggctcac cgccaatgat ttttgcccct cgagaggtgt
     5401 tccaacttct ccttcaaaaa catcaaaaag gcccacacgc tcaagtttaa catgctttgg
     5461 aaacgttgct tgccgtgtaa gtttttgcag taaaatctgc atcagttttc ccgcttcaaa
     5521 atcttttttt actaaaaacg caaaatcgcg ttccacaggt tgaaaggggg caacggaagg
     5581 ttttgctttt ttatgcttaa gcgcaggaag attctccatc atgatttcaa aggcacacag
     5641 aggaccgtca agatcaaatt cttttgcaac acgtggatga atttgcccaa aatacccaag
     5701 aactttcgtc ccctgagtga gcgctgctga gcgccctgga tgataatatg agggggcttt
     5761 ctctgaaaga gtttgtatat tattttcgct gatgccaaag atatccaaaa catccatcat
     5821 atgtgctttc atgtcaaaaa catggatgtc ttgttgtttt tcccgccagt gccgcggatg
     5881 atttttcccc acaaaaagag ctgtcgcatg gagtctttga cgatccttaa agtgcgttcc
     5941 ataaacatgt gcaatctcaa aaagaggaat cgtctcttgt gcacgttttt gattgtcaaa
     6001 agcaacccgc agcaaactgg gcacaaggct tggccgcatt gtcgtaagtt ctgtcgtaat
     6061 cggatttttt aaatgaataa gcgtgtcagc atccgtggaa tcacaaaaag atgccgccgt
     6121 tttcgcatca ataaaagaaa aattaatcgt ctctatatag ccaagattga cgagcgcttg
     6181 cctgcatact tttgatgcat gcgcactcgg cacaggcatt gtcatatgct taacaggcaa
     6241 cgaaacttct tgaatcacat caacaccacg caaccgcaca atttcttcga ttaaatcaat
     6301 atcctcactc aaatcatgac gccatgaagg gactgtaaca gtgagttgat ccatatcctg
     6361 agactcaatc gcgcatccaa gaaaccgcag catatgcccg atgcgttcaa tgaaaaaagt
     6421 ctcgcctgtg acacgaacaa ttttttctaa ggtcagtgta atccgatggg gggtataaga
     6481 aattttatcc ttcacccagt gcgtttgact aatctcgccc ccacactgat tttgaatcat
     6541 acaaagggct tgtgccattc cctcttcgac cattaaagga tccacgccac gctcaaaacg
     6601 cgtgcgagca tcagaaagaa tggcaagttt ttgacctgtc tttgcaattg taatgggatc
     6661 gaaccatgct gactccaaca aaacattgcg cgtctcgtta tcacaccctg tgcgtttgcc
     6721 gcccataatc cctgcgagag agataattcc ttcttcgtca caaataacgg gcataccttc
     6781 ggatagagta tacgttttat cgtccaaccc ctcaaaagtc tcgccttctt ttgataaacg
     6841 tagtgttata gtacccatca ctttgtcagc atcaaacaca tgcaatgggc ccccaagatc
     6901 taaacaaaca taatttgtca catccaccaa agcagaaaga gaacgcagac ccacagcctt
     6961 catacggctt tttatatttt gagggctttg tccgttcgtc acacctttta tcagtgtaag
     7021 accaaactgg taaaaaacgt cgggcgcatg attagtcaca tgaaaagtgt gattaaactg
     7081 tgtttttact tttggtgctt taaaagcttt cagcgttaca tttttatttt ttgttttaaa
     7141 ataagcataa agatcccgcg cgatcccata cacagaaaag caatccgcgc gattgggtgt
     7201 gatggcaata tcaatcacag gatcatcaag gccaagagcg ctgacgatag gactccccag
     7261 cggcagatcc gtctcaagct ctaaaatccc ctcacaaggc acatcaatca aaagctcatc
     7321 agaagagcaa agcatgccag ggctatcgat accacgcact ttacccgcct taagaacttg
     7381 gcctgttgac gggatcacaa caccgacttg cgctaaaaca actttgatgc ctgcacgagc
     7441 attctttcca ccacaaacaa gttcaagtgt tttttgtcct gtattcacgg tacaaatttg
     7501 caaacggtcc gcttcgggat ggggacgtac ggcactgatc tctgccaaaa taaaatcttt
     7561 gaggcgctcg gcttcatgcg tcacatcttc aacctcaagg ccaatattca caagcccctc
     7621 aagaacatcc tcgaggacaa aatccccctc taagtaatca cgcagccacg cgtacgttaa
     7681 tttcataaaa aacgagcctt tcattcatta tactttatgt gtagcagatt tatgggcatt
     7741 gagggaagaa cagcctttgc aaaaaaagat aattatcttt caaaaaagta ttctctgctt
     7801 tataagtagt gcataaaatt ttttaaaata tttctttgat ttatggatac aaagcaacgt
     7861 ctcgccttcg cctttcgcat tctcgctcat ctcggtttgg atgatcatac ctacacacat
     7921 ttatcggcgc gctcaaaaca tggtttttac ctcccggctt ttggctaccg ctttgaggag
     7981 atcacaaaag atcttttgat tgaagtcgat cacaatggga aacctctcga tcccacgtca
     8041 agtcttaata ttacagggca aattatccat caaagtattt ataaaaaacg ccctgatatt
     8101 tgcgctgttt ttcaccttca cacaccggca atgatcgctg tgtctgccat ggaaaaagga
     8161 cttttgccgc tcagtcagtg ggcgcttcat ttttatgaac aagtgaatta tcacgactat
     8221 ggtgctcttg cactggaagg agaccaaggg gagggcatcg ctaatgacct tggcgataaa
     8281 agggtcttga tgttgcgcaa tcacggtgtg attgtgtgtg gccgtacact tcacgaagcg
     8341 cttttttacg cgtatcacct ggagcgtgct tgccaaacac agtgtttagc gctctccagc
     8401 cctaagcctc ttatcattcc cgaccatgcg gtatgtgtgc aaagctgtca cgagctttta
     8461 tcctttgaga aagaccttgg actgcgcgat tggcttgctt gggaaagggt tttagtccca
     8521 taaaattatt tttttatcca cacgccaagg atttctgacc gtcaagtctt ttttttgcat
     8581 ttatgttaaa aaaagttctt gcgttatggc gttgattttt gcacgaatca atgttttcct
     8641 agtgtttcac gaggtgtgcc ttaaaaagta gcacaaatgt caaggccctg tgtgcgtctt
     8701 aaaaagcact ttcctcacac atgttcccca caaagttatc cacagattct ggggaaaact
     8761 taaaaaatcc ttaagaaacc tctgataatc ttcagcaaac tattgagaaa gtgacgtagt
     8821 gcaattgttt tttaaccgat gaaaaacgaa ttttaaaaaa taaaaaatta ggatccccaa
     8881 attttacatt ttgcagcgcc ctcaccctcc aaaagcaaat cggtaagcgc ccacacaaga
     8941 gcatccacac gatcaggcga tttgcttgta acacctggca cataactaca catttgttct
     9001 tcgagcaagt gcagcgggcg actatgaaac acgcggcctt tttcataaag agcggcaatg
     9061 ggctccgcac gcatggcctt gccccgcgtg gcacgtactt ccctataagc aacttgcgca
     9121 tcgactgact taatcatacg ccccaccaaa tcacccccct tgttcacctc tgccacaatg
     9181 cggtcagcct tatactgatg ataggcctga acggcgcgct gtccccaatc aaggggcgaa
     9241 tgtttcccgc taaggtcatc aaggatatac gcattttttt tctcatcaat acccgcaaca
     9301 acgatccctg tttcatcgga gcctgcatgg tgtgttgttg caggatcaat tgccacaacg
     9361 atacgatcca aaagaggaat gtggcgatta tcatactgag gctgctcgta atgaatactc
     9421 gagcgctgcc aaagagcacc ttgaatctcg ctcaggatct cgccataaag ttcttgggag
     9481 ccaagttttg ttcctttaaa ttcctcgaca acttgttgga taaaaagggg cgacagattt
     9541 tccctattat caaaagttgt gcctcgcgtg acatgaacgc ccttttgtcc taaaagtttt
     9601 tgaagaatgg gcagaggtcg cggcgttgtc gtaataatgc accttggttt ttcgccaaga
     9661 cggagcgata acattaactg atccattaac ttgtcgggat agcgaaattt tgcaagttca
     9721 tcaacccatg caaggtcaaa ttgtggccca cgcaattgat caacttgatc gccaccaaac
     9781 atcacggcga ctgcaccatt tttaaaatgt atttctcttt tgctcatctc aaggcgtggc
     9841 tgattttttg tagaataggt actcaaaaga ccgcttattc cctccaccat aattgagcgt
     9901 gtttgtacaa tggacgcgcc aatcaaagca atacgcctat agccgtgagc gtcaatcaat
     9961 ttgcgaagac tctcagcgcc tgttcgtgtt tttccaaaac cacgacctgc aagaatcaac
    10021 catatgtgcc aatcatcatc gccctccatg aggggcaacc tttgattctc ccgcgctagc
    10081 atctcccagt tattatcaag ccgttggcga ttttttttta tgtcttttaa gtattgaaga
    10141 acctgttcat aggacattgt aaaaactccc ttatgcatct taaaggacga gtgtgtcaga
    10201 ccttttgaaa ggcttcgata cttaaaaaat cccaacgttg aaatcctgaa aaaattcaga
    10261 agaaatccaa aatttggtga gattttctat tttgacttta aaattcaaat aaaaagcgta
    10321 taataaaatg cattatttcc tctttttttt atatatcgca tgcgcctttt ttttctttgc
    10381 ctttcttttc tttgtttagc cgcttgttct aagaaatcgg atcatacatt acgtgtgggc
    10441 atgacaaatg gtccccatgc agaaattatt gagtatgttc aaaagcgcgc caaagccgag
    10501 ggattagacc ttgaacttgt cccatttgat gattttattc agccgaacgt tgctttggac
    10561 gcgggagata tcgacgttaa tatttatcaa cacctcccct ttttacagga gcaaatgaaa
    10621 tcacggggct atgattttgt cgtcgtcggc aaagcgatta cacttcctct gggtatttat
    10681 ggtgcggacc atgtgacgtc tttaagcacg cttaaacatg ggacgaaagt tctcatttcc
    10741 gtggatccaa gcaatgcagc acgctcccta atgcttttag aaaaagcggg gcttttgacg
    10801 ctctccaatc atattaatcc cagcgttaaa gatatccaag aaaacccctt gggccttaaa
    10861 attattgaag ttgaggcgcc actcattcca cgcctgctca aagatgatgc agatattgcc
    10921 gttattattg cggattgggt gatggtttca gggatggatg cgaacaaagc gttgtttaaa
    10981 gaagacgcta aatccactta tgcaaatctt attgtcgtta aaaaaggcag tgaaacacgc
    11041 gcggatattc aaaaacttct tcacatctac caaagcgatg atgtgaaaaa atttattctt
    11101 gaaaaattca aaggggctat actccctgca tggtaaagct agcgtgacaa aaacttgagc
    11161 aaggaagttt cgagcttccc cgtgcttccc gctccttact aagggtaaat tttattacta
    11221 aactagcccc aaggtaaatt ttattatttc gccgtcaatg ggccaagaat catgatcatt
    11281 tggcgcccct caagcttagg cgcatgctcg actttagaaa tttcttcgaa ctgaaggcgc
    11341 acacgctcta aaactcccat gccaatttct tggtgggaaa gctcacggcc acgaaagcgc
    11401 atcgcaattt taactttatt tccctcaagg agaaagcgct caatcgcctt gcacttaatg
    11461 cccaaatcat gcacatcgat catagggcgc agctgcacct ctttgatttc aataactttt
    11521 tgcttctttt tagcctcagc ttttttcttt tgaagctcaa atttgtactt accatagtcg
    11581 agaattttac acaccggtgg ctcagcattc ggcgagacct ccacaagatc aaggcccacc
    11641 cgcaatgcat gctgcaacgc ttcttgcttt gacacaaccc cgaccatttc accatccgca
    11701 tcaacgaggc gaacagtttg agataaaatc tcgttattga cgcggggacc gtctttggat
    11761 tgttgatcgt gaggacgtgt aataagtctc tccatactat aaaaaatgca cacgtcaata
    11821 taccatgtct taaccttact gaacaatgga tttgtttgaa aaagagtgtc tgtttgtttt
    11881 ataattgccg catgagtgcg ttgagggcaa aataatatga cgttttattc ggtgacttgt
    11941 ccacagtgcc aaaaaaatca tcaagtcccc gaagattacc tcaaagataa ggatgcacgc
    12001 ttagtcaagt gcgcacagtg ccaacatgtc tggcgctatc tgaagaaatc aggttttttc
    12061 aatacatcgc gcttacggtt tcccttttca acgccaccac tgagtgccac gtatgattac
    12121 cgaggaaaaa ttcagcatca ctcgctttac agctataaat ggcgccgctt ttttatcaag
    12181 tgggggtcgt tgtcaattct tttggtggcg agtttttttc tatttaaaaa agatattttg
    12241 tcgatacccc cagaattttc tcaaaaagcc tatgcttttt tagataaaac caaaaatcag
    12301 gcctatcatt tcattcgctc ttttttaacg caatcagagc ctgcaaaaga aggcataaca
    12361 ttgaatgtgc ttacttctca tctatccatc attaatggtg aacctaccct gaggattaaa
    12421 ggcgaagtga tcaatcactc gcaccaagat attgatcttc ccgtgataac aataaaactt
    12481 atggacacga atgataaagg aaacggagag ctcgatgtaa ggcactcatg gctttacaaa
    12541 cttgaaaata tgccccttag agcaggcgag catcgctttt ttgaaacgac ggggaattgc
    12601 cttcaaaata aactaccctc aacagtcacc cttcacttta atgaaaacga ataagagaaa
    12661 aatttcatga caatgatcga ctatgatacc tttgagcgtg taaatttgcg cagtggcact
    12721 attatcaaag tagaggaatt cccaagagcg aaaaaacctg cctttaaaat atgggcagac
    12781 tttggtcctg agatcggtat tttgcaaaca tccgcgcagg tcacaaaaca ttatacgccg
    12841 gaaactttgg tgaataagca aattgtcgga tgtgtgaact taggcgaaaa aaatattgca
    12901 ggctttactt ctcagtttct tttagtgggt tttagcgacg tcaataatga catttgcttg
    12961 atcacaacgg atcaaaaagt ccccaacgga caaaaaatgt gctaagacgt aattcttaat
    13021 aagaagtaga caaaatgagc actgccttga cccctcaaaa aatcctatgc atcggcgatt
    13081 ttatgttgga cctttttatt gaaggtgttg tggaacgtat ttcacccgaa gcgcccattc
    13141 ctgtgctcaa ggaaaaaaat cgtttttata cactgggcgg tgccgggaat gtggtgcgca
    13201 atctagcgtc actcgggctt aatgtcgtag cgataggtgt tttggatagt catgatgcag
    13261 ggaaaaaaat tctctctttt acaaaagatc tccccaatgt taacgcttgt tttatcacag
    13321 aagctcactg gacaacgcct cttaaaacac gcctttcatc caatggacaa caacttctgc
    13381 gctttgatca cgagaaaaat aatactcttt cacaaacatt agaaacagaa atttatgagc
    13441 ttattgacca gcatcttcca acagcaagtg cggtaattct atcggactat cataaaggca
    13501 cactaagcga tactttatgc aaggcgatta ttgacaaagc gaaagcgcac aatgtccccg
    13561 tgattgttga cccaaagggt aaagattata aaaaatatag tggtgcgacc cttttaacgc
    13621 ctaatcttgg agaacttaag gccaccacac ataaaaatct ttcctctcat gagtctattg
    13681 ttcaagcggc aaatgatttg cgagatcgct atgatgtttc agcggttatt gtcacgatgg
    13741 gggcagatgg aatgatggtt gcccaggggg atgatcacta cattcatatt ccctcaaaag
    13801 caaaagatgt ttttgatgta tccggtgcag gtgacacagt tatcgccaca ttaacctatg
    13861 gtcttgtgca acagttatcc cttcaagagg ctgctatgct tgcgaataaa gcaggaggga
    13921 ttgttgtggg taaaattggc acagctgtga ttcaaaaaga tgagctgttt gatcaaaaaa
    13981 atgattcaga aaaaattatg aatctttcgg atattttaga acaagtaaaa cgctggaaat
    14041 cacaaaacct tacagttggt tttacaaatg ggtgctttga ccttttgcac ttggggcatt
    14101 tgcatcttct acgtcaatgt aaaaaccaat gcgatcgtct gattttagga ctgaataccg
    14161 atgcgtcgat tcaacgcctc aaaggaccga cgcgtcccat tcaaaatcaa gaggtgcgct
    14221 ctcaaatcct agcggccctt gaacttgtgg atgcggtgat tctttttgat gaggacaccc
    14281 cccttgcgct tatcacgtct attctacccg atgttctcat caaaggatcg gactacagcc
    14341 ttgagaatgt tgtaggcgcc aaggaagtca tagacaatgg ggggcgtgtt tacttagccg
    14401 agctgaaaga tggtttcagc acaacaagta cggtgaaaaa aatagcgctg gtcgggtaaa
    14461 ttcttcaaaa agaaaaaaat gagttcacaa aaaacatttt caaaccacac tccccagtct
    14521 ttcagaaaaa ctgtagagcc tcatcataaa aaagtaagaa tcaaaataag aaaacaatat
    14581 caatgtagtg caacaaaata ttaacttggg cttatcgcga tcatcaacta tttttctaat
    14641 agaccagagg tgatatccca ttagcttaag tttttggaca actcatttgg tacaggtagc
    14701 cagtctactc agaaaaatgt ttgacaatcc tcaaggctga ggggttttgt tgattacttt
    14761 aagtgacatc ttaagtcaaa tgtatgatgc tttttttcct gtttttcccc gaatttttct
    14821 cttttatgga actttttttg aggataatta ccccgtattt gagttgtctc gcatgagaaa
    14881 tacgcgtttt ttgagcaatt tttccaataa ttttcatgaa atttcagatt ttttaggcaa
    14941 aattggagta ttttttgtgg gttttcatga aaattcggta att
//
LOCUS       DAIAHG010000007        14234 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_7_length_14234_cov_3.007841, whole genome shotgun sequence.
ACCESSION   DAIAHG010000007 DAIAHG010000000
VERSION     DAIAHG010000007.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 14234)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14234)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14234
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7_length_14234_cov_3.007841"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    8555..8564
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3873 a         2942 c         3277 g         4132 t
ORIGIN      
        1 tagttttctt ggcacagcat ttattcaggc agtggagcag cttacagggc cgcaaaaaaa
       61 cctcaaggcc atcgatattg atggaaattt cgatcatgat acaaaatgga atctttttat
      121 ggaaaccctt gagtcgctgg atcagggtga aggggttttg gtgctcgcag atctgtttgg
      181 gagtacaccg tccaatatcg ctattgcggg gctggctgtg agtaaaattg aggttgttgt
      241 gggggtgaat cttccgatgc ttattcatgt ccttgaaaat cgtcaacgga atactcttga
      301 ggatcttgca gagtttgcaa aacaagcggg tcaaaaggat attaaaattg cgtcgacatt
      361 tctgactgaa aatcctttag acacaaaaga gctttctcaa gcgtgaattt tttgctccct
      421 aaaaagacca gtttctatta gaatgctcaa agaaaaagcg tagaaaaatc aaaaggagag
      481 atcatgtcgc ttacccatga ggatattaag aaaattgcga atttagcacg catacgcatt
      541 gatgcgtcgg aatatgatgc cgtgcaacaa cagctttcag gtattcttgg atggattgat
      601 caattgcaac aagtggatac agaaggtgtt cttccctacc gcgatctttt ggaaacatcg
      661 acgtatgagc gtgaggactg tgttgttgag gatcatcaag aggcgcttat tttagcgaat
      721 gctcctgaga aggctcataa catgttcgcc gttaataaag ttgtggaata gaggtattga
      781 aaaaaattat gacagattta acaaagctca cgcttactgc ggctaaaaaa ggtcttgcgt
      841 caaaggcctt cacaagtgtg gagatcacac aggcgcacat tgctaagatt gaaaaaaatc
      901 gcgctcttaa tgcgtacatt accgataatt ttgaaggggc gcttgctcag gcaaaggagg
      961 cggatgcgcg ctatgaaaaa gggaatgctg gaaaacttga ggggttgccc cttgggatta
     1021 aagatctttt ttgcacaaaa ggacttcgca cgacagccgc ttctaaaatt cttggtaatt
     1081 ttgttcctcc gtatgagtca accgtcacgc aaaatttatt ggatgcgggg gctgtttttt
     1141 taggcaagtt gaatatggat gagtttgcga tgggctcagc caatcttacg agtgcttttg
     1201 gtccgtgtat gagtccttgg aaacccaaag aggatgcgca caaacaaatt gttccgggtg
     1261 gttcttcggg gggatcttcg gcggcggtgg cagcggatat cgcactagcg gcgacagcgt
     1321 ctgatacggg aggctctatt cgtcaacctg cagcttttac aggcactgtg gggattaaac
     1381 ctacgtatgg tctttgctca cgctatggca tggtggcttt tgcgtcgtcg ctggatcagg
     1441 cggggcctat tacgaaaacg gttgaggacg cagcattgat gctggaggtg atggcaagct
     1501 atgatctcaa agatagcaca tcgcttgatg tgaaaatccc aagctacaca cagcatttaa
     1561 cagaaaatat taaaggcctg cgtgtgggca ttgtgaagga atatattgat catctcaaag
     1621 acgaagcttt ggagcttttt catcaaggag tggcgtggct gaaagaggcg gggtgtacgt
     1681 tcaaagatgt atcgcttcca acaacacctt atgcactccc cacatattat attattgcac
     1741 cggctgaggc gtcggcgaat cttgcgcggt atgatggtct gaagtacggt ttgcgcgttg
     1801 agggtgagac actggatgat atgtatgagc gcacacgccg cgaaggattt ggagacgagg
     1861 tgcgtcgtcg tattatgatt ggtacgtatg ttctttctgc aggatacttt gatgcgtact
     1921 atatcaaagc gcaaaaaatt caaacactga ttaaaaatga ttttgatcgt gcctttaagg
     1981 atgtggatgt tttattgacg cccacgacac cgacggatgc atttgcgatt gatgaaacgc
     2041 caacggatcc tgtgacaatg tatcttaatg atattttcac tgtgaccaca aatcttgcgg
     2101 ggcttccggg gatttccgtg ccagcgggac tttctaagcg tggtcttccc ttaggattac
     2161 agcttattgg acccaaatta tccgaacaag ttttgtttaa tgcggcgctt gttcttgaaa
     2221 aagctgcagg ttttaaagga tctttcgatg ctttaaaaca tattcatcat taagaaaagg
     2281 gagaatttta tgacaacaga tcattttaaa gaaatccctg tggagccgca taatcttgtg
     2341 aagggaaaaa ccggtgagtg ggaggtcgtg attggccttg aggttcatgc acagattatt
     2401 tctaaatcta aattgtttgc cacggcctct gctgattttg gcggtgatcc caacgcacat
     2461 gttggatttc ttgatgcagg gatgcctgga acactgcccg ttttgaataa agtctgtgtg
     2521 gatcaagcca tcaaaacagg gcttgggctt agtgcaaaaa ttaatttgct ctctgtcttt
     2581 gaccgtaaaa attattttta tgcagatttg ccccttgggt atcaaatttc tcagtttacg
     2641 cgtccgattg tgagcgacgg atatcttgat attgatgttg agggagagac aaagcgcatc
     2701 ggtattacac gtcttcacct tgaaatggat gcggggaaaa gcattcatga cctccatcct
     2761 acaaaatcat atattgattt taatcgtgct ggcgttggtt tgatggagat tgtatcagaa
     2821 cctgaaatac gctcaagtgc ccaggctcag gcgtatgtga aaaagctccg tatgattttg
     2881 cgttatcttg gaacgtgtga tggcaatatg gagcagggca gccttcgtgt ggatgcgaac
     2941 gtttcactgc gcagaccagg cgaaaagttt ggtacgcgcg ctgaaattaa aaatgttaac
     3001 tccattcgat tccttggtca ggccattgag tatgagattg atcgccagat ggatattctt
     3061 gaaagtggcg gggaaattgt ccaagaaaca cgtctttttg atccgaataa aggcgaaaca
     3121 cggtccatgc gttctaagga aaatgcccat gattatcgtt attttcctga tccggatctg
     3181 ttgccccttc gtttgacaga agagcgcatt gacaccctaa aaaaagcgat gccagaactt
     3241 cccgatgcta aaaaagcacg ctttatggag gcctttaacc tgaccgcata tgatgcggat
     3301 gtgcttgtgg cggaaaagga aacggccgat tactatgatg cggctattgc agcccttacg
     3361 gcgaaggata aaacagctgc cgctaaaatg cttgcgaatt ggcttatttc agaagttttt
     3421 gcagcgatga accgtgatca taaaaccctc gcgtcctttg cgccagaggc tcttgcggaa
     3481 cttgttgatc ttattcagga caatacgatt tcggggaaaa tagccaaaga tgtttttgca
     3541 aagatgtggg aaagtgggaa atccgctaaa acgcttgtaa gcgagcttgg gttgcaacag
     3601 atcacagatc cttcagcgat tcaagggatt gtggaggata ttattgccaa aaatcctgat
     3661 aatgttgcag cgtacaaagc gggtaagcaa cagctttttg gtttttttgt cggtcaggcg
     3721 atggctgcga caaaaggcaa ggcgaatccg gcacttgtca atgaggtatt gaaaaaaata
     3781 ctggaggaat agaaccttaa agatttgcgg gagaggttgg ggagtttatc aggctctcct
     3841 ccttcataat ctctaaaaag gttgcaaaag ctatatttct tccgcagaca atcgccgcga
     3901 tttttttgcc tttgagtgta tgtgcccttt taatggctcc tgcaacagca acacctgcgg
     3961 ctccttcaat gatcatcgat tcattaatcg cgagatattt tatcgcttct ttgatgtcag
     4021 cttcactcac aagaactgta tgatcaataa gtttttgaca aagggcgaag gtgatagaat
     4081 ctttttcaat cccgccagct gttccatcag aaagcgtttc tttttcatcc acattgatga
     4141 tggcaccgct tttaaggcac tcgtacatgg atcgtgcctt ttctggccaa caggcaaaaa
     4201 cacgaatatt tggaaagtat gatttgagat aagcagccgt tcccgagaga aggccacctc
     4261 ctcccacggc tataaaaata gcatccaatg atccacttgt tgggagattt caaggcctat
     4321 ggtgccttgc cctgcgatga catctaaatc attataaggg ggggatataa ggtgtttgtg
     4381 tgcggtgtgc ttcgatcata gcaatatctt ctggaagagc actctcctcc accagcgtca
     4441 cgttcgcgcc caatgcacta agtttttcgt atttatacgg agaaagagtc tttggtgcaa
     4501 aaatatgtgc tttgagatta aatttttccg ccagtgttgc aacagcaagt ccgtggttgc
     4561 ccgaagaagc cgtgattatt ttctcatact ttttcgcgtc aagcagtcgc agtattttat
     4621 gagacgcacc acgaatttta aaagatcctg tgtattgaca attttcacac ttaagaagga
     4681 attgtgtctt agtcagatct gataagaaat gacttttgag aagcggcgta tacgctacat
     4741 gctttcggat acgcaaaaaa gcttcctgga tctctcctgg aagctttttg aggaaattgt
     4801 ttgctcttat tgtcaaactc taacccaaga tattaatcgg tttctttggc ccgctctgcg
     4861 cgtttgcgat cgttgggatc cagaagaatt ttgcgaaggc ggatgcattt tggggtcact
     4921 tcaacgcatt catcgggttc gatgtacgca atcgcttgct ctaatgtcat gattttaggc
     4981 ggagtgagac gaatcgcctc gtcctttccc gatgcacgca cgttggtgag ctgctttgca
     5041 cgcaatgcat taatctcgag gtcattatca cggttatgct caccaaggat catcccaagg
     5101 taaattttat caccgggttg gacaaacata atgccgcgat cttcgagctt ccacatggca
     5161 taggccacag cttcgccgtc accattagaa atgagcacac cgttacggcg cccttcgatg
     5221 ggaccgcggt aagtatcata tttgaggaaa agacggttca taatacctgt gccgcgtgta
     5281 tccgttaaaa attgcccgtg atagccaatc aaaccgcgtg agggggcatg gaaaataata
     5341 cgggtttttc caccgccaga ggggcgcatg tcgaccattt ctgccttacg catggccatg
     5401 gcatccacga ctgtcccggt ataagcgtca tcaacatcaa tatgaacctc ttcaatgggt
     5461 tccatgcgga caccattaac tgtttgataa agaacctttg gacgaccaat cgatagctcg
     5521 aacccttcgc ggcgcatggt ttcaatcaaa acaccgagct gtaattcacc gcggcctgcc
     5581 acatcaaaag catcgcggtc atcactttct gtaatacgaa tcgcaacgtt cccctcggcc
     5641 tcgcgcatca aacgatcacg aatcacgcgg gaggtgactt ttgtgccttc acgccctgct
     5701 aagggagagt cgttaataga aaaacgcatg gagagcgttg gggggtcaat gggaagtgct
     5761 gcaagaggct cattcacttc gggcgccata actgtttcag ccacagtagg tttctcaaga
     5821 ccggcaatag caataatatc gcccgcttcg gcaacatcca caggggtgcg ctcaagtccg
     5881 cggaaggata aaattttaga caaacggcct tgctcgatca gctcgccttg ttgattcagg
     5941 acttttacgg gcatattaag gcgcgctgtt cctgagtaaa tgcggcccgt gagaatgcgg
     6001 cccaggtaag aatcatattc acgtgtcgta atcagcatgc gaaagggggc gtttttatca
     6061 ccgcttacaa tcggaacatg ctcaaggaca agatccataa ggggttcgag agttccgcct
     6121 tggtgttcta aatccatgga ggcccagccg ctgcgtcctg aggcatagac aatgggaaaa
     6181 ttaagttgtt catcatgagc atccaaagac ataaaaagtt caaacacttc gtctaaaact
     6241 tcgtgggcgc gtgcatcttg gcggtcaact ttgttaatca caacaatggg tttgaggccc
     6301 agtttgagtg cttttccaag aacgaatttc gtttgcggca taggcccttc agcggcatca
     6361 acaaggagaa tgcagctgtc caccatagag agaatgcgct ctacctcgcc gccaaagtca
     6421 gcgtgtccag gtgtatccac gatattaata cgtgtgtctt tccacatgag ggatgtacac
     6481 tttgccaaaa tcgtaatgcc ccgctcgcgc tccaagtcgt tgctgtccat ggcgcgctct
     6541 tgaacatttt ggttgctgcg aaaggtgccg ctttgcttga ggagaccatc cacaagggtc
     6601 gttttaccgt ggtcaacgtg ggcgataata gcaatattac gaatatcagt catggggttt
     6661 taaaatccta aaaaaagtga ggttagcgcc actaatgctc caccatagaa gcaaagtctt
     6721 aagaatgagt taatcattca tagccaaaat aacaatagcg cacgtgaaaa attaaagttt
     6781 taccactcta ccttattcga aagatgaatg aaagaatgtt tttaaattta cgtctttctt
     6841 tagttctttt ttttaatttt attgagagtt aattgccgct tgaggggaga aaggcgatca
     6901 atatacaaaa tcccgtcaag atgatcgatt tcatgggaaa aagcacaggc taagaaatct
     6961 gaaaaatcct cttcttttag gatattatca cgatcaagat agcgcagacg aatttttttg
     7021 gggcgtttaa caaccgcatt tgtattgggg acagaaaggc aaccttcgtt ccaatcggct
     7081 gtttcactat ctttccaaag aatttctgga ttgcccatgt gataaagttt tttctcacca
     7141 tcgatgtcta tctcgacaac aatgacacgc ttgtgaacgc cgatttgagg ggcagcaagt
     7201 ccaacaccat cttcggcaat catcgtctcg accatatcat ccataagggt ccgaatctca
     7261 tcatccacac actctatggg taaggatttt tttttaaggc gtgggtcggg ataggtgagg
     7321 acagtgcgaa cagtcatgaa atgtggggct ttctcgtgtc aaaaattaac ggttttcatc
     7381 actaatatag ttgttttttt ttatttttct agtttttctg cagagaaata cttatttgcc
     7441 atagattatt gcgtcacaaa aagattcagg cagctctata aatcgatctt gaaacgtttt
     7501 ggacgcaata agatgagtgt tattttttta tgcttttttc aatgtatcta acgaattttt
     7561 tatttatttc cttagtagat agttttttat ttttgggcgt tttagaatct tttgttaaat
     7621 caataatctt gttagtgtgt tttttttgtc tttttttggt cgtatttaca aatttttccg
     7681 tacttttaat agcattgcct tctttgtcaa aattcacaaa tacataagta aaattctttt
     7741 tttcataaac aattttattt ttattaaatg tcacatttac aaaattataa tttttatgat
     7801 caggtttttg attacaatct tctttgtttt tcgggttagc taacgctgtt gtgagtatta
     7861 aaaggctgag gtaaataaat ttgactagta ttttcatctg taggttcatt tttcatgtaa
     7921 ataatgtgca tttatctcat gattattaaa aaaattaaat agtaaaaata atttattgat
     7981 aagatatata cgataaaaaa aagaaggaca caaaatatgt gaccttcttt ttagttttat
     8041 tcgtgcttta ttctttttta tgtatccaag aagctgcgca gtttgcgtga gcggcttgga
     8101 tgtttgagtt tacgtagcgc ttttgcttcg atctgacgaa tgcgctcgcg tgttacggca
     8161 aattgttggc ccacttcttc gagagtgtga tccgtgttca ttccgatccc aaagcgcata
     8221 cgcagcacgc gctcctcgcg gggggtgaga ctggagagaa cccgcgttgt cgtctcacgc
     8281 agattcgcgt gaatagccgc ctctacgggc agaacggcgt tcttatcctc aataaaatca
     8341 ccaagatggc tatcttcctc gtccccgata ggtgtttcaa gagagatagg ttctttggca
     8401 attttgagaa ctttgcgcac tttatcaagg ggcatcaaaa gtttttctgc aagctcttcg
     8461 ggtgtgggtt cgcgcccaat ctcatgcata atttgacgtg atgtacgcac gagtttatta
     8521 atcgtctcga tcatatgcac aggaatacga atcgnnnnnn nnnngtgcgt gcttggtccg
     8581 caatcgagcg tgtaattgcc tgacgaatcc accatgtggc gtaagttgaa aacttatacc
     8641 cacggcgata ttcgaattta tcaacagcct tcatcaaacc aatattcccc tcttgaataa
     8701 gatcaaggaa ttgcaatcca cggtttgtat attttttagc aatcgaaatc acaagacgga
     8761 ggttcgcctc gatcatttct ttttttgcac gcgatgcttc gcgctcgccg cgttgaataa
     8821 tcgtcacaat tgcacgaaga tctggaaaag gaagcccaac acgcttttca acatgcgcca
     8881 tttctgcaat cagtgcttcg atgtcgtttg tatggcgtgc aaggaaattt tcccattttt
     8941 tatccttaag agttttaact ttgctcagcc aatcatgatt aatcacctga ccattaaaat
     9001 gttttaagaa agcttcgcgc ttaaggccac tattttctgc gagagtcaca agaccgcgct
     9061 cgagattggc aacacttttg ttcaaattcg tgtgttcttc gagtaaatca tccacgcgca
     9121 tcatattcag cttaagatca gagagcatca caagaagttc ggattttatt ttttgatact
     9181 ctttatgctc cgaaaagctt ttaactttag cctctttgtg gagtgtatcg cgaagtgagg
     9241 caagctcctt ataaaggctg agatagctct caaattgatg caaaaccttt ggcagcattg
     9301 aggcttcata gtccgaaagc tcgtgtacaa ccgtatctgc gtctgcacca gcatctgcgg
     9361 attccgtgga tgattcttcg tcttctgtat ttgtgtcttc atcatcaaaa ccactattgg
     9421 tatcgatagt aatcacatca cgcaaaagaa ttttgctttc actcaagagc ttgaaccaat
     9481 gcgcaatagc tttttctgtc acaggacttt cggccaagcc gcccagcatc atttccttgc
     9541 caaactcaat acgtttagca attgcaattt cgccatcgcg ggaaagaagc tcaacgttgc
     9601 ccatttcacg caaatacatg cgcacaggat cgtccgtccg accggcggta tcctcaacct
     9661 cttcggcctc ttcgttgcca tcgaaattaa attcactgcg cggtgcaatc gcttcattgg
     9721 ttgttgtcac aagggattca tcgacttctt catcagagtc cacaatgcta atgccaattt
     9781 ccgcaatcat cgtcatggct tcatcaatct gatccgacgt cagttgatct tggggaaggg
     9841 cctcattaag ctcatcataa gtaatatagc caagctcctt tcccttggcg ataagacggc
     9901 gcaaacctgg attcgatttg ctgagctcca tcaaaggaat atccgtagat tcggcttcct
     9961 ctgtgctacc atcccgacga tttgttttga gtgtcataaa atctccttta gcagtgaaaa
    10021 atcttgcaag tctgtttgta tttgtttaag tccgttccag gtttgaatat cccagtgttc
    10081 tttgagttta tttttgtaat catcgagtgc tttttcgagg cccatgcgta cattatagcg
    10141 ttgccacaca tctaaaaacc ccgcttcagc ctcttcggtt gttgcggctt cctttgagaa
    10201 gggcgctatg gccataagag aagcatcttt tttaagcgtg gtcaccaagg cattagtctc
    10261 aagggcaatt tgtgcttttg ttgcaagccc ttcgtcgcca cactggcttt ctataagtgt
    10321 ttgaaccagc tccttttctt cctccccctc aaataccaca cgcacaagct cttcgacaat
    10381 tgttggtatt aatgtagggt gattgagcaa agttacaagc aaaatttttt gagccagagg
    10441 acctgagtca ggcattgtga tttctttggg ggtattaata agcgttttag agggggctga
    10501 gtgatttttt tgagtggact caaagattct tgcatagaag gcttgtttgt aaaaaccccg
    10561 aatatcctta tcttcgatgg tctcgatgga ttttaaaaaa tcctgtttta tctgggcttt
    10621 gatttcgggg atttcaaggt gtgtcttttg ggcataatgg tcgcacaaaa tcgtaatcat
    10681 aggtttggcg tgaggcaaaa aagacgcaaa agccgccgca cctttcccgc gcaaaaggct
    10741 gtcgggatct tcgcccgagg gaagtgtgat gaattgaaaa gaatagcctg gcttcaaaag
    10801 agggagcgca tgataggcgg cacgaatcat cgccttatgt cctgcggaat cgccatcaaa
    10861 acaaagaaag ggtgtgtagc tatagcgcca taaaatctgc atttgttcgg ttgtcagcgc
    10921 tgtgcccaga ggcgctacgg cattaaaacc gttgatttgt gcagtaattg catcaaaata
    10981 cccttcaaca acgatcaaag gacgattctc ggagacattt ttaatattat tcaggccgaa
    11041 aagcgtcatt tttttatgaa aaagttctgt ttcagggcta ttcatgtatt taggttgctg
    11101 gtccttgtgc aaagatcgcc cgccaaaagc aatcacgcga ccctttgtat catgaatggg
    11161 aatcatcagt ctgtcgcgaa agcggggaag aatctgttga ctgacacttc cttcgatggc
    11221 gaggccggct tggatcacct ctgaagtact aaatccctta gctattaaat gcggcacaag
    11281 atcctgtgtg gcaaaaccca gtcggtagtt ttgaatcact tccgcaccga ggctccgctg
    11341 agttaaatag ttttgtgcca aagtattttt agcaaggttt ttttgaaaaa acagcgtcgc
    11401 ttcctctaga agactgaaaa gacgttcgtg cgtatcttta tgatttattt ttttttgagt
    11461 gatggcgggc agagcaatac ctgccatttc acacagttgc ttgagggctt ccataaaagg
    11521 aagcttatga taatccatca taaagcggag aatatcccca tgagcgccgc agccaaagca
    11581 atgataaaat tgtttgggat cattgatggt aaaagaaggg gttttttctt cgtgaaaagg
    11641 gcagcacgcc tgaaactcat ggccatgtcg tttgattttg ataaaacgtg cgataacacc
    11701 tgataaaggt aagaggtctc gaagtttttt aataaatggt gtgaattctg acatggaggc
    11761 ccttacgata tcaccttata ctaaaggaaa acactattaa tgactagggg tatcattcga
    11821 tgaagaggca atggactgaa acggcgatga ttcagtccat tttagcaatc agtttgtaga
    11881 cttatctttt gtggagtgtg ttgagataat caacgtactg ttggctaatc tcattgagga
    11941 aagcaatgaa gagaaaaagt ctacagaagt ttagggctcc atgttggata aagggatcgc
    12001 ttgagagagc cgcaatggca atgtttgtaa aaaaaccaaa tttcgttcca atgtcgagat
    12061 ggttgtagag tgtgttaata tttttattca cctctaattt ttcaatcttc ttaagaattt
    12121 tctctgattt accgatgacc tcttcaatgt tttcttgagt gtcttgacgg ttttgaagga
    12181 atgtttttgt gttattctca aatgacgcct tatcatcctc gcgagcgtga gcaatagaaa
    12241 tgacggaaag ggcaagaaga agaattttga gcatgtgtga tcccttggtt gtttttataa
    12301 tcgccccacc ataccctttc agaatttttt taaaaagcaa aaagcgctat taatattcat
    12361 ttttagagaa ggagaaacgc tttaactttg gctatttttc agaacaagtg tgatccagcg
    12421 atttctgtca taaaggcgat ggagcgaaaa tccctgttca agataagctt tttcaacatc
    12481 ttcctgttgc caatcgagga gccctgagag aataacatac ccatgcggtg caagatgctg
    12541 atgcatggcg ggcgctaaat gaatcagcgg ttttgcaagg atatttgcga taataaggtc
    12601 aaatgtttca atcgttttat cgaggccctc gccaaggcgt gcttcgataa gcgggctgat
    12661 attattaagc gcagcattgc tcatggtgac gcggacggcc tcagtatcat tatcaatggc
    12721 aagggttttt acaggattaa gtttgcacgc agcaatcgct aaaatgcctg aaccacatcc
    12781 taaatccaat ggattttccc acttaaattc atccaagaga tcactgaggg caagcataca
    12841 cccggaggtt gtaccatgtt ctccacttcc aaaagctgtg gctgcgtcaa gaataagagg
    12901 gattttattc gtaggaatat cccctttgaa atgactgctg tgaataaaaa aaggaccgac
    12961 atcctgagga gggaattttt cccacatctc ctctagccaa ttattttcca tcaaaggttg
    13021 aatggtaatg agcggttctt ctatgccaag ggtttttgcg gcaagaagga tttgcatgcg
    13081 caaatctttt tcattaggtt cctcaagcat atggccttcg acaaaccaat cctcagagtc
    13141 ccagtcagcc ttaggatctt caatttccac ccaagacgtt gttacacaat gatcctcgat
    13201 cacagcactg agcgcttcga ggtagggttg ctttgtttga ataataactt gccacataat
    13261 aattttattt tttcttgaaa agtgaggttt gtctaaggct atagagagcg atgaaaatca
    13321 tcaaggaaag tgcgaaaaga caaggtacaa cccagtgccc atgaagacta tagaatgttg
    13381 gtgcgatggc ctctggtagg ctaaagtcaa taatgccaat atcattaaga tcgagtttat
    13441 gcaaaatgcg tccataaggg tcgatcacgg ccgaaatgcc attattggca acacgcacaa
    13501 gggggagtcc ttgttcaata gcgcgcacac gcacatttcc cagatgttga tgggggccat
    13561 aagagtcgcc gtaccacgca tcattcgtaa gattcaaaag ccactgggca ggcgttttag
    13621 aagctttgtc tttgggggag aaaatctctc gtgaaaaaat agcttcgtag caaatgagag
    13681 gcctaaacgg ggggacattt ttaatagcaa tgatattatc ccttgtgcca ggaacaaaat
    13741 ccttggcgcc atgcgttatt ttattgaccc ccggaaaaag aaaacgaaaa ggaatatatt
    13801 cgccaaaagg gacaagatgt tttttgtcat aaagagcttg gatctctcct ttatcattca
    13861 aagcaacaag gctgttgcgg ggggagtcac cgtcctcgcg cacactgcca aggaaaacat
    13921 atcctccttg gggggcagca tttccaagca ttttttgaat ttccgggtat tttgaaaaat
    13981 caaaagttac agccgcttct ggccataaaa tcgcctgcac aggcttttcc gcatcaagtt
    14041 gggatagaag gccttgtaat tgcagatggc gttgtgtttg ttctggatcc cattttgctg
    14101 tttgctgaat gctgggttga atgatcctaa aattataagg tgtgagaacg gtgtcatgag
    14161 caaggcggag ataaccacaa agggccagtc ctacgagaat cgctgtcgaa aaaaaagtat
    14221 aaccccatgt gcgc
//
LOCUS       DAIAHG010000008        14138 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_8_length_14138_cov_3.424792, whole genome shotgun sequence.
ACCESSION   DAIAHG010000008 DAIAHG010000000
VERSION     DAIAHG010000008.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 14138)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14138)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14138
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8_length_14138_cov_3.424792"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    8347..8356
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4642 a         3270 c         2793 g         3423 t
ORIGIN      
        1 gaaagactcc ccatccaacg gtttttcaaa aaaagcatgg gcgtcaagtt gattaacgtg
       61 ggaaatattt ttgtaattgc tcatggaaga atgaatcaca acgggaattt cctttgtttg
      121 aagaaggccc ttgaggcgct gtaaaaccat ttttccattc atactcggca ttacaagatc
      181 gagaatgata atatcaggct tctcctttct ttcaagatgt ttcaatgctt catgcgcatc
      241 tgtgtaggaa gagaaagtaa aattagcccg acactctgtt tttaaaattt ctttgacgag
      301 attaatatct tttttgctgt catcaatcat catgacgtga atgtttctat tattcgttgt
      361 aacctcatca ctaaaacgca tacttttaaa tgagtagtac tttggatgat tatatgacgg
      421 ttgcgtagag ttccacataa aaaatctcct ttcattagcc taccattaat aacagtgtta
      481 aacaaaagtg actctattca ggccacaaat ataaatctaa ctcaaacaag taaataaatg
      541 attaacaatc actagagttt tatttatatt tttagctatt gtggggaaaa attaaagagt
      601 aaacgctgtt taaatcgtct caggcacaag gcgtagtgcc atactaaagt aaacaccatc
      661 attcaattgc gccctgcggt aatcaaagct ttcaaagtga atccctggca acggttttgt
      721 cagcgtactt ttcattgcgc ttaggtctgc attttgaata tacgtcccaa acgtcgtgtt
      781 tttgtcaagt tttcccgttg tatcttcaaa gctaaagtag cgaatcttgt atttttgatg
      841 attaatcgta atgattttgc ccacaatcgc tttgtccaca aaagcatttg attggctcat
      901 ttcgatttct ttgcaggctg tgatcagttg ttgcagcata agcgccccct cttgagtatc
      961 gatcacaaca cgttgaggct ttgtctccgc acacaccgtg ttcataacgg gaaaagtaac
     1021 aagggataac agaataaata gtttgagcat agtcacactt tctttcaagt ttattgaaaa
     1081 aattttagca caggccccaa gagaccatca aaaaccctcc agcgtattta aaatagctgt
     1141 ggttttttca aaatacgtcg taggcagctg gtcttcctcg gcccaaaccc aagcaagcac
     1201 cgcttgcaca aaacaccaat caaggattcg ttgataaggc aattcaagga gctcagcaaa
     1261 gcgcgtgacg cgaccctgaa tactatttgg cgcatctacg tgatgcagta attctggtat
     1321 gggattgcgg ataaaagcgg caacctcaaa ggccctctca ccgattaccc cttttggatc
     1381 gatcaccacc caatcattcc cctgccacag aatattatca tgatgcaaat cgccatgcag
     1441 gagggtatct gcaccacgtg tttgcaataa aaaatcgcgc attttttttg ctttttgaag
     1501 ggaactctct gggatacatg ttgctgtgtc caaggccttc agccaatctc tgatatgggg
     1561 gaaaggatga ttttgaggaa tcgcagacct atgcaaacgc tgcataagag aacaggcaat
     1621 ctggatgctt tcctcatctt tgtgcggaaa aaaagactta agcgacgttc caggcacagc
     1681 acgctcgagc aagagcatgc ccggctcctc ggcaatagca gtcacagttc cacgatcttt
     1741 aaaacaccga agagccatgg cttctcttgc aagacccgcc gtatccatac ttcgttttaa
     1801 aatgacaggc accccttttt gaaaacctga ggctacataa ttgtaggtta gagaagggaa
     1861 cggtgtaagc tctgtcagat tgtattttag agccatgtca cgcagaaaac ccgggagctc
     1921 ctctaaccac ttcgcgccct ctttgccata tattttttct atcgtcgtct caaaagaaac
     1981 gacggatgtg cttttagtca tgggtgtttc acaggagatt cttttttagc cttatgccac
     2041 tgattaataa tgaggcccgc aaaaacaagg aggcaccctg aaaatgttgt gagcgtatac
     2101 gcctcgcgaa aaaccatcca cgaaagcgtc ataccaaaaa caggaatcag caacgcatac
     2161 ggtacgacaa tcgctgcatc ataaagttta aaaagatacg cccaaagcgt tgacccaatc
     2221 catgttccgg cgacgacgat aaaagccaac gagagcacac ccttcatcga aatatggcgc
     2281 aaactttcga ttataagctc tggtccctca aaaataaaac ttaggcctaa aaggggcaaa
     2341 ggcgggacaa gccccatcca aatcacaagc ccaaaagcat ccacattttt tgcctttcga
     2401 aaaaacatat tcgcaaaagc ccaaaagagg gctgctatca taatacaggc aaaccctatg
     2461 atattcgcct gtccccctgt ttgctgcgcg attagcccaa tccccataaa agcaatgacc
     2521 atgccaaggg cttgccgcgg ggaaacctga cttttaaaga agattttcga cagaataatg
     2581 gtaaagagca cttgtgtttg cagcaaaaga gaggccagcc ctggggaaaa ttcaaaatag
     2641 agactcagaa aaacaaagga aaacttacac accccaagaa gaagcgcgat agtcgacata
     2701 agggaccacg aaagattctt aggctttgga atgaaaaaaa tcaaaggaaa tgttgaaaat
     2761 ataaatcgca aacacaaaag taaaaaaggc ggcaggtcat tcatacacca cttcgccacc
     2821 acaaaattac tcccccacac aaaagcgatc gtgagggcca aaaaaacatg tctaatatgc
     2881 ataaaaatta atgaaatgag ggcgaaagaa cagctgccat tctacgggat gaaaccatct
     2941 tccacaagag agaaacatcg ttattcccac cattcgcacc aaagtttaag gagagaaata
     3001 tatgtattag aatactcaaa ggatgcaaaa ctatgttaga atgcacagat agaaaaaaac
     3061 ttttacacac atccatgcaa aaaaaatcca ttttttggct cattctcctc atcactgctg
     3121 tttttttagg cgatgttctc tccaagcaat gggtcgttgc ccacatgacc acggacaaag
     3181 tatggcccat cacatccttt tttaacatcg tccttacggt caatcatggg gtaagctttg
     3241 ccatgctgtg ggccagccat atctatggcg tttatgcgct tattgggctt acaagcctgt
     3301 tatcagcggt tgtgctttat tttcttttca tcagccccac atttttcgaa aaaatggcct
     3361 tctcccttgt ttttgcaggg gcccttggca atctttttga tcgcattcgt attggcggcg
     3421 ttgtagactt tttagacttt catatgctag gttaccactg gcctgccttt aatgtggcag
     3481 actcagccat ttgtattggc gtttttttac tttttcttta ccatttcgtg ttacaaaaaa
     3541 aataagagaa gcaggtttta ttcattatga cgtattatta ctcccttttt ttcatgctaa
     3601 gctccctagc ccttttgctc tcaggatgcg acactgttcg cagcaccctg ggccttgatc
     3661 actatcaagc agaccctttt aatgtgccca caaatccgcc cctgacactc ccccctgaat
     3721 acacactccg gcctcctgcc cccggcgctg cgccgacgtt ttcaaaaaca tccaacacac
     3781 aagcccagca aacgctctcc acctacaatg ctcaggtcta ttcaagcaat gccacagaaa
     3841 atggtcttgt cggacaaatg aaaacaggga cacctcctgc caatattcgc gaacttgtgg
     3901 ataaagaggc ccatgaggaa agtaatctgg gtgggaaact tggcaaacaa gtggatgatt
     3961 ggaagacgca ggccaagaaa aatatcgcaa gtatttacac aaacgatggt gcagaaaaac
     4021 agaaggactc ctcccctcct ccacaacctc aggatccaaa aacaacctca tgatcgaaag
     4081 aataattgca tccatcatag gttttttagg gagagccctg atcatagcac tccccctcgg
     4141 ttcgttgggt gcaaaggata tgcaaagcac aacgcacatt gtctcacccc ctccacaaaa
     4201 aattttagat cacccactcc accttcatct tccctttaaa ctttctcctc caggcacatc
     4261 ccttcaaagc ataaagattc aagatattca cacgcctctt ggttttcatg gctggcttgt
     4321 ggaaaataaa gatatccccg ttgtgactgt cgagtttatg tttgttgatg cgggatctaa
     4381 aaatgaaata aaaggtaaaa aagggcttgt cgcactccta gcgtcactta tggatgaggg
     4441 ggcgggcgac tatacaagtg atgattttaa aaaatttctc atcacgaata atattaatct
     4501 tattgtcagt cagggagttg ataattttat tttaggtttt tctgtgccta aaacgcatgt
     4561 cgctcaggcc tttaaggcca tggaccttat tcttacacgc ctaagatttg actcagaacc
     4621 cttagagcgc atcaaaacac atcttcacct ccattataca cagagaaaag aaaatgaggg
     4681 tgaacggggc atggaggaac tcgaacaaac gctcatggcc ggctcacctt acgtatcgtc
     4741 tcttgatgat cgtgtcgcgg atcttagaaa cataagtgca acagatctta aagatttcat
     4801 gaaaaatcac tttaccaaaa acaaattaat tatctctgta tgcggtgata tttccacaga
     4861 cgagctgtca aagctcattg atacatcgct aaaaaacctt ccacttaaag caacacccga
     4921 agcactccct gatgtaagtt ttcaaaacct tggatcgtta caccctattt ttatggatat
     4981 tcctcaaagc cttattcttt tcgcccaccc gggatttaag cgcagtgatc ctgattattt
     5041 tgcacttgat cttgcgatgg atattcttgg aagtggcgag cttgggtcca ggttaatgaa
     5101 ccgtgtccgc gaagagaacg gccttgtcta tggcattggg atcacaaaac ctatttataa
     5161 acattatcaa ctcattttag gaaatgcttc tacccacaca gaaaacgtca caaaagtaat
     5221 tgatcttatt cgaagtgagt ggaaaaaatt tatcgcagca ggcgtgacca aagaagagct
     5281 cgatcgtgca aaacaacgct ccattggtct ttttccacta cggctcggaa atacgcaaag
     5341 tatcgcctcg ctgctgacat ccattcaata ttataacttg gggattgact accttgaaaa
     5401 gcgtgcacaa gccatcaatg atgtgacgct tgagagggtt aataagctta ttcaagaacg
     5461 ctttaaagat gacgggctca cctttgtgat tgtaggccgt gaaaactctt cacacgcacc
     5521 gatacaaaaa atcaaaggaa aatcgtcatg acctatctcg tgcgcttttt tctttggata
     5581 atcatcacct ctgttagcgt tgctcaaagt gctgcctctc tttttaatca tcaaagtttt
     5641 acattagcca atggcctcaa ggtttttatc ctccccaacc atctctcgcc gcttattcat
     5701 gttggtcttt attataaagt aggtaccgcc gatgatgctc ccgaagaaca cggtttgtcc
     5761 cattttctcg agcatatgat gtttaaggga accgaaatga tcccaagggg tatgctggac
     5821 acacttcttt tgcaacaggg cgttgcgtat aacgctcaca caactcccga ctatacatgc
     5881 tacgaagcca cgtttgctaa aaatcaactg gagcttattc tttttctcga agctgatcgc
     5941 atgacgcatc ttaattttac acgcgcagat attgaatctg agcgcaaggt tgtttttgaa
     6001 gaacgtgcca tgcgccttga taatcacccc tttggaaaag tgattgaaat ttatctaagg
     6061 tccctttatt ggaaacaccc ctatggcatt ccggctattg gctatccaga aaatattgca
     6121 cactatagtt ttgaatctgt ccgcaaacac tacgagacct attacacacc caataatgcg
     6181 attcttatta tcgcaggaga tactaacagc gccacggtaa agccccttgt tgaaaaatat
     6241 tttggcccca tcccttttaa aaaagttcca gaacgccttc gccctaagga accaagccat
     6301 gagggaacgg ttatacgcat tgaacacaca gacgcccgta atcatgccat tgtcctgcaa
     6361 tattcttatc atgcacccag tgccgtaagt gaggggaaaa ggcatgccct gccccttatt
     6421 cttctcacac aaattctctc gggcaatgaa ctcagtcgcc tttggcaaaa atttgtgaat
     6481 caagaaaaaa tcgctgtgaa cattgatttt tcatatgacc cttggagtat tgaccctgaa
     6541 acacttgata ttattatgac actcaatgaa ggagtcacca aaaaatacgc ggagaaacgc
     6601 ctagaggaag agatccaact tctcttaaaa aaaggtgtga cccctgagga attagctcat
     6661 gcaaagcgtg atattctagc cagtcttgcg tttattaaag atggccttga aagcactgtc
     6721 caaatgtttt caagagttgt ttttggggtt ccagaagaag atattgagca ttttgatcag
     6781 cgcattgaag ccgtaactct tgaacaagtt aatgaggcga ttcatgatgt ttttgataaa
     6841 aaacccgaag ttatcctcac acttttaccc aaaggttatg acctgtccaa agaggataca
     6901 cctcccctgg cacaatcttt gtggaacgcc ctgcgctcta ttttttaaaa actatgcagt
     6961 tttaagagcg cattttgata aaaagcatgg ataaaaaact aaataaaata acagcaccac
     7021 actgatgcaa aagagcaagc ttaatgggca caatgtaaag caccgttata atcccaagag
     7081 tgatttgaag acttacggct aagaaaagaa gaacggcctc tgtcttataa cgcaataccc
     7141 aaaggcggtt aatcagccaa aggcaactcg cacctgtgag cattgcaagc acacggtgca
     7201 cccattgaac cgttgcagga ttttcaagaa aattagtata gactggcgtg taaaaaagcc
     7261 actctgatgg cacccaatcc ccccccattt taggaaatgt gtcatagagt ttccccgcac
     7321 gcagccctgc gacaaacgcg ccataaataa ttgtcacgct tgtgagaaaa aaagtaatgc
     7381 gcagcggaaa attaagcttt tgaggcggtt tttttcgatc agggtacaaa gataaaagcg
     7441 cactcaacat gaaaaataaa agtaaaaaag ccaggcttag gtgagccgca agacggtagt
     7501 gactgacata aggattattc acaaggccac ttttcaccat gtaccacccc atccctccct
     7561 ggatcatccc cagggctaaa atcaccaaaa gtttgcggcg caagggcttt tcgcagtgtt
     7621 tccaaaaaaa gaaaaggggg agaagaaaaa caagcccgag aaaacgcccc aacagccgat
     7681 gactgtattc catgtaaaaa atgaatttaa aatcacttag catgagatcc atgtgcttaa
     7741 gttgatattc gggaaaagct ttgtatttct cgaactccaa cgcccatgcc tcttcagaaa
     7801 gtggagggat aatccctgtg ataggtttcc actccacaat cgaaagtcct gattctgtca
     7861 aacgcgttgc ccctcccagt aaaatcatcg ccacaacaag taaaatgcaa agcaaaagcc
     7921 aaagcgagag agggcccctt aaattatttt tttttaacat aaatttgaga ctaatttaaa
     7981 taaaactaat ttagcttaga aaaacacatc ccctgttgca aaggttttta tctctcccgt
     8041 ttctgttttt aaaagaagac atccctcttt atcaatatta tgaaaggatc ctttgataac
     8101 acgatcccct tgttgaatcg tcatcatcgc gtgacgcttg tcccgcaaag atatccactg
     8161 atcacacaaa tccggccagt ggccttgcgc taagtcgttc aaatatgcca agagataagt
     8221 tttaaggccc tctaaaagat cacaaagagc aatagaatac cctgatgctt tgagggatgt
     8281 ggcaggatac aacacctcga caagctctgg cgcgtgctct ctattcacgc ctatgcctat
     8341 ggataannnn nnnnnnaaca cagaaaaatc ctgagattct tcgaggattt cgatgagaat
     8401 ccctgctatt ttttttccgc ccagcaacac atcattgggc cattttaggg ataaatcttt
     8461 taccccttga gtatcgagtg ctttataaag tgcaagggct gcaacaaaag aaataaatgt
     8521 tttatgttgt aacgcacaag gcaagacaaa cgtcgcaaaa agatttccct tggctgaaac
     8581 ccatgagcgc ccccgccgcc ccctcccctt ttcttgggac tgcgccgtga cataaaaagg
     8641 aatgggtgtt tttttctgat aaagatcacg tgcaacatcc atcgtactgg acacatgatc
     8701 aaacgcatgc aaagaaagtg tcatgtgtaa aaaagcgtcg acgccgcaat attcgtccag
     8761 ttcaaaaggt atttaggcag gaaaataagc cctgtgagaa gcgaaataga cagaataacg
     8821 ataaggtagt taagaccaag tttgccagaa acatgcgtta agattgatgg ctcaacaggc
     8881 ccatcaaaaa acattatttt gaggatatta agataataat aggttgttat aaccgttgtc
     8941 agaaccatca caatagccac attgtaaaat tccgaaccaa cggccgcctg tagtaacaga
     9001 agttttccca aaaaccctgg aaaaggaggt aatcccgaca ttgtcagcac aagaatggca
     9061 atagcaattg cgacaaaagg cctttgtgtc gcaagacctt taagatcaga aagtttatcg
     9121 atagtactgc cccgacgaag caaaatcatg acgcatgtca aaaacccaat catcgaaaag
     9181 atatagagtg ttgtgtaaaa aagacttcct cttaagcctg attcattcgc aaaagcaagg
     9241 cccacaagag aaaaaccaat gctactaatg gatgcatacg ccagaaaccg cctcacattg
     9301 ccttgcatga cagaggccac agccccaaaa accatcgagg ccacggcaag gatcatcaaa
     9361 aggggctgcc acgtagaaat ttccgtataa aagggcccca gaaaaagcct catcataaaa
     9421 ccaagcgctg cgattttagg ggctgtcgac acgagaacaa gaagcggata gggtgtgcct
     9481 tgataaacat ctggcgtcca catatgaaaa ggaaaagagg agactttaaa agcgagaccg
     9541 cacagtaaaa aggcaatacc aaggtaaaga ctcagagggg actgagcgcc attattgagc
     9601 aaagattcaa gcacattaaa atttgtcgtt ccagaaaatc cgtaaataaa acttattcca
     9661 aaaagaataa aacaactcgc cacagctcca aggataaaat attttaaaac cgcttccggt
     9721 acattttttt cctcatgaaa aagcccaatc atcacataga gggcaagact ttgaatttca
     9781 agaccgatgt aagccgggag gaaatcattc gcagaaacaa ggataaacat gcctaaaaca
     9841 gcacaaagaa gcagcacgac aaactcaaaa tgtttcactt taaaaaaagt caaatgggat
     9901 tgaagattca gcgctaaaag agctgcacaa agtgcgataa tactcttgat aaaaagacta
     9961 aagctgtcat taaaaaaatg gccatcaaag gtcacatcac gcaaccctgg caagaaaaaa
    10021 cagccaaaag cgatggcgag taacatcaac gaggttaagc taaaaagcca tgctttacgt
    10081 tgacctttgc taaaaaggcc tgcaaccgtt gcgcctagag cggcaaaaac gagacagact
    10141 tcaggagacg ctggaagaaa atagctgaga actgtcatcg tgctggattc cccgtcattt
    10201 tatctttttt tccctttgta ttggttaact ttggcggttt ttcttgttgc ataaaaagat
    10261 cgagcaatgt ctcatcagaa ctaaatcttt tgccataagc atcaagcatg tgatgggcat
    10321 atacattcaa aatcgatgtg acaggcttag gataaagccc taagataaac acaggcacga
    10381 taagtgctgt aagcacaaaa aactcagaaa aacgaaggtc agctatgtgc ttaatgtcta
    10441 aaaaatgagc attcaactta tcatacacaa tacgacggta aagccataaa ccgtaggcag
    10501 cacttagtat aatgcctaaa acaacaaagg ctgtgagtgt aggagacact tgaaaaatgc
    10561 caagggttac aaaaaactca ccaagaaatc cgcttgtccc cggaagccca atcgacgcca
    10621 gtgtaaaaag cataaaaaac gttgcatacc gcggcatttt tgcagcaagc cccccataat
    10681 aggcaatctc tcgtgtgtga aaacgctcgt aaacgacacc cacacaaaaa aacaatgcag
    10741 ccgaaataag accatgactc aacatttgaa taatacttcc cacaagaccg tagtggttaa
    10801 aagaaaaaat accaagtgtc acaaacccca tatgtgcgat ggaggaatag gccacaagct
    10861 ttttcatgtc tttttgggca agagccacaa gggacgcata aagaatcgca acaaccgata
    10921 aaaaatagac aaggggtgca aaatagacag aggcttcggg aaacaaaggc agtgaaagcc
    10981 tgagcatacc atagccacca agtttcaata aaatacccgc aagcagcata gaccctgctg
    11041 taggtgcctc cacgtgcgca tccggcaacc atgtatgcac aggccacatc ggaattttaa
    11101 cggcaaatgc gacaaaaagg gccccccata gccagccttg gagataaaaa ggaaaatgcg
    11161 catggtgcat ttccaccata ttcgtcgtac ctacttcgta atacacaacg acaaacgcca
    11221 cgagcatcaa cacagaccca acgagggtat aaaggaaaaa tttaaacgtc gcataaatac
    11281 gcttttcgcc cccccaaatc cctataatca aaaacattgg aatgaggaca gcctcgaaga
    11341 aaaaataaaa caacacaaga tcaagggcgc taaaaacacc gattaagaaa gattctaaga
    11401 gcaagaaaca gatataatat tcgcgcacac gcttgaggtg atagttatgc accagtgcga
    11461 tgagcgtaag gaatgatgtg agtacgatga gaaaaagcga tagaccgtca acgcccaaat
    11521 gatatgaact attaaaagcg gaaatccacg catatttttc aacgaactgc atcgttgtca
    11581 caagaggctc aaagtgatgc cacgcataaa cacttaaaaa aaagtgaatc cctgcaacaa
    11641 aaagagcaac acgcggtgta ttttgaaggc tttgattatc gcgaataaaa aaaattgtca
    11701 cggccccaag aagaggcaga aggatcaaaa ggcttaaaag cggccattgc attaataaaa
    11761 acctccttga acccatagat aaagaagcac actcatcaga cctaccatca tcgcaaacgc
    11821 atagtgataa agatagcccg tttgaagctg cttaaaagct gttgatattc gcgtcacaag
    11881 ggacgcaaga ccattagggc caaggcgatc aatcgtcaaa aggtcccctc tttgccataa
    11941 aatcgtccca agttccaaag aaccctgtac aaaaatttta gaatatattt tatcaaaaaa
    12001 ccacttattc aacgcaaaaa ggtagaggta ttttaaatgg cgcgtgagat taaatgctac
    12061 atgaggtttt gtgaaaaaga agaaataagc ggcaccgacg cccaaaagag ccgcaataat
    12121 gggagcttgt aaaacccaac ttggcacact tgcatggtga tgaataaaaa aatcctctgt
    12181 cataaatcca agaaaagtag cccctagcgc aagacaaata agtggtaccc gcatgaccca
    12241 gggcgcttcg tgaatatggg ccattacatg ctcatctgca cggggactgc catgaaaaac
    12301 tttgattaaa agacgccagc tataaagtgc tgttagaaac gccacaaaaa gaccaatccc
    12361 aaaaagcatc tttgcctgtg ccgttccccc tgcataggtt gccacaagga tgctatcttt
    12421 tgaataaaac cctgagaaaa agggaatccc cgcaagcgca aggttaccaa tccacatcat
    12481 ggcgtaggta aaggggatgc gccgataaag cccccccatt ttttccatat tctgctcatc
    12541 cgacaaggca tgaatcacag cccctgcccc caaaaagaga agcgccttaa aaaaagcatg
    12601 ggtaatcagg tgaaaaaaag ccgcatcaac gccgcccatt cccgctgcca taaccatata
    12661 gcccagctgg ctacacgttg aataagcgat cacgcgctta atatcctctt gcacaagtgc
    12721 tactgtcgct gcaaagaaac acgtaacacc cccaacccag gcaataaaat gctgaaccga
    12781 taccgccatt tcaaaaagag gatgcaggcg aataagcaaa tacactcctg ctgtgaccat
    12841 ggttgcagcg tgaatgagtg cagatacggg cgttgggcct tccatagcat cgggcaacca
    12901 cacatgcaat cctatctgcg ctgattttcc catcactccg aggaaaagag ccatacatat
    12961 taaactcacc gcatcaaccg aatagccaaa aaataaaaat gtttttccac tcaagagccc
    13021 taatgtcgat ggttctaaaa tccgctgtag atcaagcgtt tgtgtcataa aaaacaaagc
    13081 agcaagcccc acaacaaggc ccgcatctcc cacacggttc accacaaatg ctttaatcgc
    13141 agcactgttc gcagacggtt tatgaaacca aaagccaatc aacaaataag aagcaagacc
    13201 aacaccctcc catcctaaaa aaagctggac aagattcggt gccataatca gggaaagcat
    13261 aaaaaacgta aaaagactta aatacgccat gaaccgcgga atcgatgcat cgtgggacat
    13321 atacccaacg gaataaatgt gaataagcgc tgacacaaca ttcaccatca ccatcatggt
    13381 aaggctcgtg cgatccatca tcatcccaaa ggtcatattc agagaatcaa cgtcgatcca
    13441 ttgccaaaaa gtaagggttg ttgttttaaa agcgataaac agaaaaatag aagcgacggc
    13501 tgcaatcccc ataaaactac aggagagcca ttgcaccaag cgatcactta cacgtttatt
    13561 caaaaggatt gcaatgaaaa agcccacaag tggcgaaaaa aaggcatagt agggtaagta
    13621 gtgcgccatt agttcttcat cccttcatct gacttagatc atccacacgg atagagcgat
    13681 gctggcgata atagacaacc agaatagcaa ggccaatcgc cgcctctgca gccgccaccg
    13741 tgagaataaa taatgaaaag acctggccga ccatatcatg gaagtcactt gaaaacgcca
    13801 caaaattaat attcgttgca agcaacatca gctcaatcga catcaacatg agaataagat
    13861 tgcgacgatt gagaataatg cccaaaagcc ctatggaaaa aacaatgaca ccaaggatca
    13921 aatagtgctg taagggaata accatatcaa tcatgaggat aatcctttgc cgggtttgac
    13981 gttcaccatt ttcagtgtct gcgcaggatc gcgttccaac tgacgacgaa tgtcctgacg
    14041 cttgagtgtt tttttcgtct taagtgtcag agcaatcgca cccaccatcg cgacaaaaag
    14101 aatgatgcct gcaagctgaa acgctaagaa ataattgg
//
LOCUS       DAIAHG010000009        13815 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_9_length_13815_cov_3.176227, whole genome shotgun sequence.
ACCESSION   DAIAHG010000009 DAIAHG010000000
VERSION     DAIAHG010000009.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 13815)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13815)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13815
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9_length_13815_cov_3.176227"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3953 a         2890 c         2984 g         3988 t
ORIGIN      
        1 cacagaagct ttactgtgat tgaaggggcc cttgtcttag aggtggtcga taggcttaaa
       61 aaagataagc ttcttgcggg aactattacg cgtatccccg aggaagggcg ccttttgccg
      121 gcaacctatc cctatcatcg tggagaagat cgccaggtgc ttattgatcg tatggaaaaa
      181 gcgatggagg atactttact tgagttgtgg gaaaagcgcc caggggattg tctttttaaa
      241 aatccttccg aggctttgac aatggcatca atcattgaaa aggaaagcag tcaaatcatt
      301 acagagcaac cccgtatcgc aggtgttttt aaaaatcgta tgcttaaaaa gatgcgtctt
      361 cagtcggatc ccaccgttat ttatggcctc acacgcggtg aaaaaccctt aggccgtgca
      421 cttacacgaa cagatttaaa agttgattct ttttataaca cctatttata tttaggtctt
      481 cccccagcac ctattgcctg ccctcgcaaa gaagccatcg aggcagctct tcaccctgat
      541 gaacacgatg agttttattt tgtggccaat ggcaaaggtg gacataactt tgcaaaaacc
      601 ctcgaagaac atctaaaaaa tgtgcaaaaa tggcgagaga ttcagcagca aaaacattat
      661 acggctgaga gtaaatagct caaaaatagt gagaaaatat cgatgaacac caaaaattct
      721 attcacagaa ggggcctatt actggttctc tcatcgcctt cgggcgctgg caaaacaacg
      781 attgctaaaa aattgctcga gagtgagcaa catcttgata tgtctgtttc tgtcacaacg
      841 cgtcctcctc gtcccggtga aaaaaatggt gtcgattatt tttttgtgga tgagcccaca
      901 tttcaagaga tgctcctccg tgatgcattt ttagagcacg caaaggtttt tggaaactcc
      961 tatgggacgc tcaaagaacc ggtttttaat actctgaaaa atggcaaaga tattattttt
     1021 gatattgatt ggcagggcac acagcaattg gcgctttatg cgcgcacgga tcttgtgagt
     1081 atttttattc ttccgcccag cgtcaaagct cttgaagaac gcctcaataa acgggggcaa
     1141 gatacgcatg atgtgatgcg tacgcggatg gatcaagcgg ctaatgaaat gagccactgg
     1201 gcggaatatg attacgtgat tattaacagg gatttagaat caagtatcct tgaggtccaa
     1261 aaaatcttgc actctgagcg tttaaagcgt acacgtcagc ttggtcttgt ggattttgtt
     1321 aacacactca ggcaagagtt ataaaaatca gttttttatc aacctaaaat caaagaaaaa
     1381 aacaacttcc gggaaacttt gaagaaatta acttaagaag ctctaatgta gcaaacgaat
     1441 gctttgataa tgcctgtgac aagattttga aaaacaaagt tcttaaaaaa attgatctga
     1501 gtggcaatgg gttggatgaa aagagtgaga actacatatc gattcaagaa gtgctcgaaa
     1561 aaataataaa aattaaaaaa tgcatgggct tagtttgaga acccacgctt cttatctctc
     1621 taaaggatca ttttcctgcg ctttactttt aattgagctc cggatctttc gaccaatcaa
     1681 tcacttttcc acgggtatca tcccatcgat aaagccatga aacaccataa ggaatataac
     1741 aattgtctgg ctcagagtat gcctcatcct ccattgcatg atctaggatt ataaaattat
     1801 actcaagttc acgggcgatt ttgagcaaat cttccatggc ataggcatta attaaattcg
     1861 catgcaaaag ccatatatgg cgaatattgc gaccaaaaat tttttcagac gcagctctat
     1921 aaaaagcaaa ctttgcctgt gtatgcgcaa gatactcagt aattattttt tctttatttt
     1981 ccggattttc cgttagtttt tgattaaact tccaatcatc tgtatcaatc gtaacggggg
     2041 caattgtata attttccttt tttaaaaaag tctcaaaggg tgcacgtatt tcttttgtag
     2101 accccgtatc aaggtaggga tgcctaaaat atttataaga gaaccctgca tcttgcataa
     2161 gtttttttga aatttttgct cccttaagga cgtcctcttg aaagtcttca gcctttgatt
     2221 ggcttaagta cggatgggaa tatgtgtgat ttccaaggga atggccatac tcaatccatc
     2281 gttttagaat cgctattttt tcctctgtcc cccccttatc atagaggttt ccctcattca
     2341 caaaaccaat ggcaggtgca ccaaaattct ttaaagcctc taaaatactc tcgctaatga
     2401 attgctgatc ttctattgat aaatcctgtg gacagggaag atcatcaaat gttatcgcca
     2461 cctcatgcgc acaggcgaaa ggtgtcacta aaaataagag tataaggata aaaagtttca
     2521 ttacgacatt actagcgtgc taggggttta aagcgaatgc gtttaggatc caacgcatcg
     2581 gcacccaggc ggcgcttttt atccgcctcg taactttcaa agtttccttc aaaccactcc
     2641 acatgactct cgccctcaaa cgcaagaata tgtgtggcac agcgatccag gaagaaacga
     2701 tcgtgggaaa tcacaatggc acatcctgcg tagttgataa gcccttcttc cagcgaacgc
     2761 agcgtttcca catcaagatc gttcgtgggt tcgtccaaaa gaagcacatt tgcccctgat
     2821 tttaaaatgc gtgccaaatg cacacggttg cgctcacccc ctgagagaag acccactttt
     2881 ttttgctgat cagcccctct aaaattaaaa caagagcagt acgcacggct tggcatctcg
     2941 cgcctgccca attgcacaac atcaagacca tccgagattt cctgccacac agttttattg
     3001 gcatcgagca catcacggct ttgatccaca tagccaagaa cgacagactc cccaagttta
     3061 aggtcgcccg catcaggttt ttcttgccct gtgagcattc taaaaagggt tgtcttaccc
     3121 gcaccattgg ggccaatcac ccccacaata ccgccgcgtg gaagcttaaa cgacaagtcg
     3181 tcgattaaaa gtttgtcgcc aaaagctttt gtaatatttt tggcctcaat cacattatcg
     3241 cccaggcgct ctccaggcgg aatgaaaatt tgaccatcgc cttgacctgt atcgtaggtt
     3301 ttgctcagga gttcctcata cgctgcaata cgggctttgc ttttggactg acgggccttg
     3361 ggcgattggc gaatccactc aagttcttga gccagttgct tttggcgcga ttgttcttgt
     3421 ttttgctcaa ggacaaggcg tttttgtttg gcttcaagcc atgcggaata gtttccttca
     3481 aaaggaatgc cttcgccgcg gtcaagttct aaaatccatc ccacaacatt atcaagaaaa
     3541 taacgatcgt gggtgatcag aacaacggtg cctttatagt tttgaagata tttttctagc
     3601 catgccacag attctgcatc aaggtggttc gtaggctcgt ctaaaagtaa caaatcaggc
     3661 attgagagca gcaagcgaca cagagcaaca cggcgtttct caccgccaga aagacgctca
     3721 acagcccagt ctccgggagg gcagcgcagt gcgtccatcg cgatatcaac tttgcgatca
     3781 atatcccatg catccacatt atcaattttt tcttggagtt ctgcctgcgt ttctaaaagc
     3841 gcattcattt catcatcaga catgggctcc ataaattttt cattgagggc ctcaaaatcc
     3901 tctaatagtt ttttaatcga cgccacaccc agcatcacat tgcccagcac atcaagagtt
     3961 gcatcaagct caggctcctg tgctaaatag ccgaccttga caccctctgc ggcccaggct
     4021 tccccttgaa attccttatc caagcccgcc ataattttta acagtgtgga cttacctgca
     4081 ccgttggggc cgatgatgcc aatcttggca ccggggaaaa aagacagcca gatatctttt
     4141 aagacagtgc gtccgccggg gtaatgcttt gagagcgatt taatgacata agaatattga
     4201 taggaggcca taaaaaaaca caattgtaag atctaagagg taccctacca tagatcatgg
     4261 tgaattaaaa agggggtagc gatcctcccc tcatcaggtt tttttttact tatttattgt
     4321 ttttatagag gctaccgaat agtaagatca cgatcgtact tttggcatgc attgtcgaga
     4381 tctcggaaac catttttggt aatagtgtta tttaaaatcc acaattttgt gaggcacgta
     4441 ttattccaaa taatttcttt aagggaatca atgcttttat cagaaaggcc cgtgtcgtaa
     4501 aaactaattt cttcaatccc tgaattcttc aggtaaagac taagcgtttt aaggccatta
     4561 tccgtcagat tattttcgcc aaggttaact atttttatat ttttattttt gctgagagtt
     4621 tgtccaaatt tttccatact tttttctgtg atataagtat tgtaaagcga aagcttcttg
     4681 acttctaagt ttgaaatctc atcacaaagg atattaagac cgttatctcc gattgtttct
     4741 ttgttaagat cgagctctcc ctcacaaaat ttttctttga caagcgtgcg tatccgatct
     4801 ttctcctttt cttcttttag atcactcccc aaaagagatg ctgtgcctgt caaaaaaaga
     4861 ataaaagcaa ctgtgtgtat acaaaagcga taaagcatca tgattctttc tcctcaaaaa
     4921 tatctattaa agtcttccac agattatttt ttatgtcgag gggatgattt cttgtaaacg
     4981 tttgagggtg agaatctcag gcaaaaccac aggctcgccc tcttttggat aaaaaacgca
     5041 aaagggaatg ccttttctgt caaaggactc caagagtgct gtaatctctg gattggcatg
     5101 ggtccagtca gctttaagga catgaacgcc atgagctttg aagaggtctt gagcattttt
     5161 cgacgctaac acatgctcat tcatttgaca cgtcaaacac cacgcagctg ttgcatagac
     5221 cagcgttggc tccttgagtg caatggcacg atcgagtttt tcttttgaat agagagagtc
     5281 atgatagcaa gattcatttt tagtgttatc actaactaat atccaaaaag gtagtgctat
     5341 actgagtgct gaagaaaaca ttgtgatttt tgtgaaaaga ttttttctac caacgttatt
     5401 ttttaaaatc catagggcaa aagctaaagc aacaagggtc caaccgctat aaaaaatacc
     5461 gcttggtcct acctcgtgta ttaaaatcca tattagccaa acaagtgtaa ggtaaagggg
     5521 aaaggccaag aattctttga gttgaatcat ccaagggcct ggcttgggaa gtctgttttt
     5581 tttgatagga agaaaactga ggcataaaaa aggaaaagca aggccaagac ctaaaaacat
     5641 cattgtcaga agaatcatcc aggcattttt tgtaaaggcg tatccaatag ctgtggccat
     5701 gaagggggct gtgcagggtg ttgccactaa tgtagccaaa atacctgtca agaacgggga
     5761 taaaaacgca tgggaggttt ctaatttttt ggaaagcttt tcgcttactt ttccccctgt
     5821 ccaggaaata tgaaaaaatc ctgaaagatt gagggcaatt aaaaacaaaa gccctatcat
     5881 aaaaaggata aagggcggtg actgcatttg aaacccccag cctatggccg tacctgtatg
     5941 ctttagcgca atcatcaccc cacctagaca aagaaaagat gccacaatcc caagggtgta
     6001 tgccacccct tctctttttt tggaggagtt ttcgtgctga ataagactta agatttttag
     6061 agaaagaacc ggaaacacac agggcattgc gttcaaaata ataccaccta gaaaggcaca
     6121 ggcgagcata agaatgtttt gtattagggg atttattgtg tgtgtgaggg tgatatcaaa
     6181 agcttttatc ccatttttca tatcaaatat caaaagccct gacgttgtgg cgatatcttt
     6241 ctcatttcta aggggcactt gaatataaaa atgattatca tccatttcaa aggtttgagg
     6301 cgcatgatca tcgacccaca gatcctggcg agggagaaaa ttaatttttt gaatagggtt
     6361 tatctcgtct tttggatttt caaaaataag agcctttggc acaataatcg tcagcatttt
     6421 ttgatgctgt gtgtaggtta aaatttctgt gattttatgg ggcgtttttt gtttgataag
     6481 atcaggaatt tctttggagc gcggctcttt tgagagaggt agattcagtg aaagtgttgt
     6541 tttttcaggc acacagaggt tcgaacacac aagccacttg gccttgagcg tgagctgaat
     6601 aaagttttta tcataatttt cagggacatg aatctctgta aaataatagg tttttcctgt
     6661 gtatccatag ctcgtgctgt gttcacttct caaaatagac ggtgttgacc acagaatggg
     6721 tcccgctttt agaccatcgt caagcgtcca ttcgaacgtt gcttcaagtc ctgcatcacc
     6781 ggggttttcc caatataacg attgattggc ttcgggttct aagacaagtg caacccaaaa
     6841 agatccgcct ttggagggat ttgaaagtgc gataagggaa gcctttgtgt ggggtgtcgc
     6901 tgcagaaaaa gtattcgcac cggttattaa aataaaaaag cttaaaaaaa agctgaagac
     6961 aacagacata gacacggagg taattctaaa aaatctattt tattaaaagc gtgatctttc
     7021 ccatcgagca agttaaaaat cccacttgct atctcttaaa acttcagaga atatcgagga
     7081 agaaggaaac taagactttg gagactttat gtcagggacg cacacacacg aaatcatcac
     7141 agaggatctt ccccgttggg acctatcgga tttttatcac tccccagagg attcaaaact
     7201 tcagacggat atggaaagtc tgcaaaaaga tgtgaaggcc ttcgtacaaa aacaaggaac
     7261 gattaaaaat cgtctctcag agctgtatga tgatctgatg atttttcaag atattgcaca
     7321 gcgttcatca aaattatcgt cttattccta tttgaatttt gcaacgcaaa tgacgaatga
     7381 aaaggcaaaa gcctttgagc aaaaagtcag tgagtttcaa acaagtcttt ggacggattt
     7441 gatttttttc acgcatgaaa ttcaagagct cagtgatgaa gatatggaaa aagcgtatgc
     7501 ttctaatgca gcattaaaaa cgtataagcc atggctggat catacgcgcg tctataaaga
     7561 tcacatgtta gagcttcccc aggagcaaat tcttgtgcaa aaagatctca catcgagtgc
     7621 ggcgtgggtg cgtctttatg atcaaacact ggcggagatg atgtttaagg accataaggg
     7681 tgtctcgctg aatctttcgc aagtgcttga gcggatgtct gatcctgagg cagcggtgcg
     7741 tcaagaagca gcagagttat tatcccagga gctttcgtct aaaaaatcac tgctgacgct
     7801 tatttttaat actcttttaa aggataagga gattaatgat aatcttcgtc gctatccttc
     7861 accttggacg tcgcgccatt tggccaatca aatcgaagaa gaggttgtgg atgcgctcgt
     7921 ggcgacggta aaggataatt atgacgtgtg tcatcgttat tatgcgtgga aagcgaagca
     7981 atttggtgtg aagcgtcttg agtattggga ccgtaatgcc ccactttctc atgcagcaga
     8041 ccctaaaaca tcgtgggatg acgccaaaaa aatagttttt gaagcgtatc atgctttttg
     8101 ccctaagatt gctgctattg ggaaaaaatt ttttgatcat gattggatcg acgttccagc
     8161 ctatgcaggc aaaacttctg gcgcattttc ccaccccaca acgccggatg ttcatcctta
     8221 ccttatgctt aattttattg gcaagcgccg cgacgtgatg acgctcgccc acgagcttgg
     8281 tcacggcgtg catcagtatt tatcgaacgc acagccctat cttttaacgc atactccact
     8341 gacggtagcc gagacggctt ctgtgtttgg tgagatgcta gcgttccgtt ccatgctctc
     8401 aaaagcaaaa acgaaagatg aaaaacgcag cctccttgcg gggaagatcg aggacatgat
     8461 caacacagtt tttcgccaaa tttcttttca tgaattcgaa gttttcttgc acacaaagcg
     8521 caaagagggc gagctttctt atgatgattt tacaaaggcg tgggaaaaaa cacagcgcga
     8581 ggcccttggc gatgccgtgc atttgcatcc gctcagccat aatttttggg gctatatttc
     8641 ccactttttg cacgtacctt tttatgtgta tgcctatgca tttggtgatt gtttggtcaa
     8701 tagtctttat agtgtgtacc tttcaaagac agttcctgac tttcaagagc gttatgtgca
     8761 tctgctcagt caaggaggaa gccagacgta tgataatctt ttaaaaccct ttgggcttga
     8821 tcctaaaaat aaatcttttt ggcagggtgg gatcgatgtc attaaggaac ttttggggga
     8881 acttgaagct ctttaatccc atagtttttc ctcttttaat ccatatttgt ctcatttttt
     8941 aggaagtatt ggtgtatttc cccaaaaaac aaggaaaaat gagctctcga tatcctaaaa
     9001 agaggcaaag actttgtaaa aaattacttt ttccacctct ttttgtgtgt ttttcaggga
     9061 tattgtgctc atttcccctt aaaatagtca ttttcctcca atttttggcg attttcggaa
     9121 taatttccct tttttgacac gaaattttag atttttgagc ctttttcttc actattttag
     9181 ggcaaaaaat cactatcaga atcaccccta aaaatcctaa gaatgtaaag ccttcatctc
     9241 cctaacctac tgaatttctt ttgtgagtgt tgacattaaa aatgtgtaaa caatgaaaca
     9301 cttcgtaata tacctatcat tgcatcgaag gaaaaaatgg cagatcctat aattaagacc
     9361 cattgttatt gatgcattta tgctgctcac ttctcaaaaa acacttaaaa actctgtatc
     9421 ctgcacgggt gttggcgttc attcaggtca aacagcgtcc cttacgtttc atggagcccc
     9481 tgcccatacg ggtattgtgt ttttgcgagg tgacctcagc acaaaaacag aggattttat
     9541 ctcctcgcat attcattccg tcgttgatac agcgttttgt acgaccctcg cgaataagcg
     9601 cggtcataag gcacggactg tggagcatgt tcttgcagcc ttcaaaggat gtggcattga
     9661 caatgctatt gtggagcttc atggcgagga agtgcctatt atggatggca gtgctgctcc
     9721 ctttgttgaa atgatcgagc gtgccggcgt tcttttgcag gatacgccga cagcaatgat
     9781 taagataaaa cgccctgtca gcgtggcttt tggggacagt aaagctatgc tcattcctga
     9841 tgaaactttc aatcttcaga tgacattcaa tgcggggcgt cgcctcaata acctatcttg
     9901 gacctttgat tttagcccaa cctttgatca ttttcgcgac catattgcat ccgcacggac
     9961 gtttggtttt tatgaggacg cagaaaaaat gtgggcagca ggtcttgcga aggggacctc
    10021 tttggataat acagttgtct ttcaagacgg catcctcatg aacacctcag ggcttagatt
    10081 tcaagacgaa atggtccgtc acaagcttct cgatgcgttg ggcgatttag cgcttttagg
    10141 gcgctcaatc caaggaaaat ttattggcgt aaattcaggc cacacgctgc actataagct
    10201 catgcaagaa gtgttgagct ctgaggataa ttattctttt taaacttttc cactgattgc
    10261 aaacgttttt tgtattcctt taatagtagt atctttgacc ctgctgggga gcgggcgcgc
    10321 attgttgctg attttgaacc atcgcagggt tttgaggttg atacatctgt ggttgaggat
    10381 agagctgttg ttgctgtggg tagtttcctt gataggaggg agcttgtggc gtttgataac
    10441 tatgattccc ctcatatcca ccatgcggca actcccgacg atactcaacg ggcggctgat
    10501 ttcctgaaga atactcctga ggttgaggca tttgctgatg tgaatgtatt tgtgaaggca
    10561 ttgatgggtg tggaggcgct ggtggtgcgt atccctgtgg caagggatgt tgagtttggg
    10621 ggggcaattg attttgccct tgatagggac cattttgcgg gttttgcggc ggcataggcg
    10681 aagagggtgg ttgtgctacc tgaccttgaa aaaatttttt agcttcttga cgactatagc
    10741 gagatgctgc ataaataccc cctacggcgt ttgtgtgcac acgatttgct gcataatttg
    10801 caaaaggttg aaggggtgct gcaatttttg actgcgctat gccaagcgta ccttccacgc
    10861 ggctcacaac attggctaaa aattcatgct gtttttcagc aaccgcacga gaagccacac
    10921 gggaaaggtc gctcctctct ccattcttac tttgaggagg cgcactctga cttgcctgag
    10981 cccatgtttg cgtgggaggc tttggacttt ctggaaatgt tttataggcg actagaaagc
    11041 ctacagcaaa gaaaagagcg cccaggaaca taagccccat cataagaccg aaaatggata
    11101 atttattcat tgcaaaccaa aattcttgaa tgatccagtg gttaataaaa ggaatttcct
    11161 taaaagactt tagaaaagaa tcacgccatt tttttgattg aacctttggc ggtgatgagg
    11221 gataggggtg aaaaaatcct gtcgtgcgtg catcaataac cttcatattt gtcagggaca
    11281 aaggtgccgc aatcacagag gggatcccgc tcattttttt gtgcaaaggg cttttcgatt
    11341 gactttttag agaagaggga gcgacaaaag cggcgtgatt tttgtgtagc acttgcatcc
    11401 gtcacccttt tttaaaaaaa attctcttta actttagacg atttatagcc gttacacacg
    11461 cttatttttt tatagtgtga acctatgatg tttttttact tctcaaaaac catgaacgcg
    11521 agtgggacgg ttgaaaagag cctctatcac atgctcaaga ggagcgccgt caataacatc
    11581 actcacagct gtgcataaag gcatatcaat atcatacgcc tttgcaagct ttagaatagg
    11641 agaaagacta tagtaacctt cactgagcgg atgtccctca ctcagaattt cttcgataga
    11701 ttgccctttt gcaagcttca tcccgagact gaaattacgg gatttagggc ttgagcatgt
    11761 tagtgttaaa tcccccacac ctgcagggcc cagaaaagtt tccaaacgtg cgccaagggc
    11821 cagtccaagg cgcgtcatct ctgcaagacc gcgcgccatc aaggccgcca ttgcattttt
    11881 tccaagccct cgctcttcaa cgatgccaca tccgatagct aatacatttt tcacaacgcc
    11941 cgcaacctca atacccgtat aatccgttgt cgggtaaaca cgaaaagttt tgtgcgcaaa
    12001 aagcttgata agctgaggat tgtgggtcgc aagagacgct gctgcgggta aattatccac
    12061 aagctctatg gcaaaattgg ggcctgaaag aagcgaaaga gggtttggaa gaatattttt
    12121 aacactatca tagggaaaca acggcaaggg atcgtgatca ataatgcctt tgcttgttaa
    12181 aataaccgga atgtctttag gaataaatgg cgtgagagca tggatgcttt caggcaaaag
    12241 ctgcgcagga atcgcaacga tgatcgcatc tgcatttttt aaaacagcgg ttgatgttgt
    12301 cgctgctata tcgtgcggta aagcaagatt tttaaaataa tttttattgg tatgatggtg
    12361 tgttatttca tcagcctgag aagcgtcttt gacaagaaat gttacttgat gatgtgcacg
    12421 gtgacatgta agggcaagag ctgtgccaaa agaccctgca cccacaacaa taatagaatg
    12481 ttttttcatt tttaggtctc aagtcctttt aagcagtaca gggattaaat cgtcgccatt
    12541 atcgatgata aaaaacaaaa agcgcaacaa ctcattttag aattttactc aaactataat
    12601 gtggtaagac cacacgcata ggcaaaaatc tcaagaggat gagcatgatg aaataggctt
    12661 ttttgatgag catcagagga agagacgtcg acgatttgca cctccatccc ctgacgaata
    12721 tgctgtgccg caagagggca ttcggatgct atatatttat tctgatgatg gatagcctgt
    12781 ttaaaaacag gtgttcctac atagagagct gtatcaaaat tttccttcat cattccccaa
    12841 gagccgccat ggcccgaaca gcgctcaata atattcacaa tcgtatcagg aataaaacgc
    12901 aggagctccg ctgcttttgc gcctatgttt tgagcacggc tgtggcaagc caaatgcaac
    12961 gtcacatgat cgccaagagg atgaggtttt tcggggaatc cctttttctt aatcagttga
    13021 accaaatact cactgacatc catcgtatgt tttgcaaggc gctgaatatt gttttgttgt
    13081 ggcaaaagca agggccattc ttgtttgagc attagcgtgc acgaggggaa aagaccaaga
    13141 atcttatacc ctttatcaat ccacggggca aaaatatccg caatatgctc ggctgttttc
    13201 gcaaccttcg aaagatttcc ttgctcaagc aaaggcattc cacagcatcc aggataaaga
    13261 acctctgtct ccacaccatg atgcttaaga agcttaagtg cagcttcagc aacggatgtt
    13321 tgatggtagt tgccatagca tgtggcataa agaacgactt tttcgccaaa accaggggct
    13381 ttttcattcg cagggataga gtttttcagc gattttgcca caaaggtttc tttttcaaat
    13441 ttaggcacaa aagccctttt gtcgatacct gttacttttt caatcatggg gcgtgtgagc
    13501 gtattttttt catcaagtgc ccaattgaca agaggtgcaa aaggcttcgc taggcttgca
    13561 tttctgtcca tcttcacgat ttctttttgc acaaagggtg tgcttttttt ctgctgctcg
    13621 acggcccgat agcgcagcat tagatgggga aaatcaatat taaacgggtg aggaggaaca
    13681 taaggacatt tgttcacaaa acacatatcg cacaaggtgc acgcctcaac aacaggttta
    13741 aaatcctcac tttttacaga atcaagctca cctgtagggc tgttatcgac aagatcaaac
    13801 agccgtggaa agctg
//
LOCUS       DAIAHG010000010        13671 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_10_length_13671_cov_4.160218, whole genome shotgun sequence.
ACCESSION   DAIAHG010000010 DAIAHG010000000
VERSION     DAIAHG010000010.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 13671)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13671)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13671
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10_length_13671_cov_4.160218"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3860 a         3131 c         2831 g         3849 t
ORIGIN      
        1 agccatgtga attcatccca ccatcaagct gtcctggggg ttgggaaaga tttacacgtt
       61 tcagccacag cccctgatgg tattattgaa ggaatcgaac acacaggcga tgttttttgt
      121 gtaggtgttc agtggcatcc tgaatatgaa atgattgatt tggataaaaa actttttcag
      181 acttttatac aggcatgccg gaaataaata aaaacatgcc tcagcaaaaa caaccatccc
      241 ccgagcgtct cagcaaattc ctcagccgtg cgggtgtatg ttcccgccgt gatgcggaaa
      301 aatatattgc tgaaggcctc attcaggtca atggaaacat tgttacaacc cctgtgacgt
      361 ttgtcacggc aaaggaccgt gttctttttg atggcaatcc tgtgcaaggg aaagaggata
      421 tccgtctttg gctttactat aaacctcttg gccttgtcac aacccataaa gaccccgagg
      481 gacgaccaac ggtttttgat aatcttccaa gggacatgcc acgcgtcatc agtgtgggca
      541 ggcttgacct caactccgaa ggccttttgg ttctgacaaa cagtggtgca ttttcccgcc
      601 acgcagagca tcctaaaaca ggatggaagc gtatttataa agtacgtgtt tttgggcacg
      661 tggatgagct taagcttcaa gaacttcaac aggggatcac gattgatcat gtgacgtatc
      721 aacccattga ggctgttctt tcgcgtcaat caggccgcaa tgcatggctg cttgtcacac
      781 tcaaggaagg aaaaaaccgc gaaattcgca aagtgatgaa tcacatcggc cttgaggtca
      841 accgcctgat ccgaattgct tatggccctt ttgaacttgg atccctcaag cctggtgaca
      901 ttaaagagat taaaaatcaa tccgcagaag atttcttgga ctaagagagc tcctgagagg
      961 attttagtct caaagaatct tcctcacagg gcctcgatct taatctttag ccttctcaag
     1021 catttttttc atcgtactga caatacgatc aagaccactt gcatcgttac cgccagtttg
     1081 cgccatatca gcgcggccac caccgccctg ccctcccaca agaggcgcta tttcgcgaat
     1141 catctggccc gcatgaaatt tttctgtaag atcctctgtc acgcccaaaa gcacactcac
     1201 tttgccttca ctgacattgg tcagcacaac aacccccgaa gaaatacttt tcttcagttg
     1261 atccataatg gttttaagat cttttgcggg ataatctgtc aaatgcttga taagcagcgg
     1321 cgtttgatga atcatttcaa ctgactcttc ggatgcacca cccgctgcaa ttttatgacg
     1381 aagttctgtg atctgatggg aaagcgcttt ttgctcgtcc atcatgcgca ctattttttc
     1441 agaaattttc tctggcgatg tctttaaaag ctggcttatt tccttaaggc ggctctccat
     1501 cgtttgtgta aacagaagtg ctgcttcacc cgcaaccgct tcaatacgac gaacacccgc
     1561 ggcaataccc gcctctgaaa gaattttaaa aaacccaatc tcccccgagt cgttcacatg
     1621 ggtcccacca caaagttctg tggagacgtc ccccatacgc acgacacgca cactctcccc
     1681 atatttttcg ccaaagagtg ccattgcccc cgcttccacc gcttctttgg gcgtttgaat
     1741 cgatgtggat gtatgattat tcttttgaat ctgtctgttc acaaaatgct caagcgccat
     1801 aatatccgca tcactcaacg gctgaggatg agaaaaatca aagcgaaggc gatcttgctc
     1861 caccaaagaa cctttttgcg tcacatgctc ccccagaata gagcgcaacg ccgcgtgcaa
     1921 taaatgggtc acagaatgat gctgcctaag gcccttgcga cgctcttcat tcacctcaag
     1981 tttgatcgta tcaccaacct taagagtccc tgtgagttgc tccaaacggt ggactatcag
     2041 attctcaaag ggctttatcg tgtcacgaat cacagcagag ccttttgcag ttttgagaat
     2101 acctcggtcc cccatttgcc cgcccgattc accgtaaaaa ggcgtttgat tggtaataat
     2161 atcaataatc tttgtgtgcg cccccacccc ttgatgtgaa atctcatcaa tcatggcccc
     2221 ctccacgaca atggctgtga caatggcttc actttgtaag gtctcatagc ctagaaaatc
     2281 cgtcgcaccg gctttttcct tgacctcaaa ccatatttta tcactttttg actcgcccga
     2341 gcctgcccaa gctgcacggg ctgactcttt ttggcgcgtc atcgccgcat caaaccccgt
     2401 cttgtcgagt tttttcccca taccacgcag cacatcttct gttaaatcca aaggaaaacc
     2461 aaaagtatca tacaatttaa aagcaacatc tcctgggaaa gcgtctgatg cccctagttt
     2521 tgccatctcc tcttgcaaga gttttaaacc gcgcccaaga gtttcacgga atttttcttc
     2581 ttcctgctta agcgtcccca gcattatatt ttcagcacgg gcaagctcgg gatagtgcac
     2641 acccatcaca tcaatcaggg ttgggactag cctgtgcatg agaggatcct tcgctcccaa
     2701 taaatgagca tgccgcatgg cacggcgcat aatacggcgc aacacatagc cacgcccctc
     2761 attactcggc agcacccctt ccgcaagaag aaaacaactt gcccgcaaat gatccgcaat
     2821 cacttgatgg gattgctgca tggttgcatt atgcgtcagg tctttagagg cctcgatgag
     2881 tgttttaaaa aggtctgtgt caaaattgct atgcacacct tgaagcaccg cccctatgcg
     2941 ctctagtccc atccctgtat ccacagaagg ttttggcaaa ttaatcatgg agccatccgc
     3001 taaacgttca tactgcataa acacaaggtt ccaaatctca acaaagcgat caccatcctc
     3061 atcaggcgag ccaggggggc cacctgcaat atgcgcaccg tgatcataga aaatttctgt
     3121 agatggacca caaggccccg tatcacctgc tgaccaaaaa ttatccaagg ttgccattcg
     3181 aataatttta ttatcagaaa accctgctat ctttttccac aaagatgcag cttcctcatc
     3241 ggtatgatag accgttacta aaaggcgcga gggatccaag tgaaaatgtt ttgtcaccat
     3301 ctcccatgca taaaaaatcg cttcttcctt gaagtaatcc ccaaaagaaa aattgcccag
     3361 catctcaaaa aacgtatgat gacgcgttgt ataaccaaca ttgtcaagat cattatgttt
     3421 tccccctgcg cgcaaacatt tttgcgaaga cgtcgcacga gtataagaac gcttttcaac
     3481 ccctgtaaaa agatctttga actgcaccat tcccgcagag gtaaacatca acgttgggtc
     3541 attacgcgga atcaacggac tggattcaac caccgtgtga tgttgatttt taaaataatc
     3601 aaggaaagtt gagcgaatat ctgtcaggcg cataaaatca tctaaaaaca aagaaacttg
     3661 ttctatttat aatcgatgca ttgctttctg tcacgtctat cgcttcagat cttttaaaat
     3721 atgcttaagg atctaagagg gatctaagat cgtcgataaa taatcgcccc gctcttagga
     3781 aatcattaac tttttctgat catactggga ggtggaagaa atacatccaa gaaagttggg
     3841 tgcgcaaagg tttccggctt gaaatgtgcg acagagctct cgccatttta ggtcaaagat
     3901 tgaaaagtgc aagcttttta atacctcttc ggacaatgtc gtgaggggaa caggagagtt
     3961 ttgtgtcgtg gtggcggtgg gtagggaaac gccacggggg atgacgtagg gaccccgcta
     4021 ggggggagtg tgttcgttga gggtagagaa gcactagaaa tgctttaatc cggcgcgtaa
     4081 gtaattatat cccgtcacaa gagtaagaat acctgcaatc cacaagagag tttcaccaag
     4141 agcatgaata tcatttgggg gattaagagc gtcacttagg agaagggtgc tgagagcaag
     4201 catttgaata tgttcaattt gtttatcctc tgcgagcgtg ctaagagcct cataaatcct
     4261 tgggatattt ccagcgattc tgccttgaaa tgtcaaagaa agctccgagt atttttcaaa
     4321 gagatctttc attttataga gctcctcttt ggcttttcca ataaggacag gttcacagaa
     4381 tttcacaact tcattggccc aatcttctgc aaagttctct tttttagatg attgaaaagt
     4441 gaaagctttc gtatccttcg cattctcttt aagcgcatca ttagaactcc aactttcaga
     4501 ggtgtgcgca aaaagtgcga aaagcagtgc tgaaacaatt attttcatga aaaaatcctt
     4561 tggggaatga acaacttttc taggttagaa tgcaagattt tacaatgcga tagcatttta
     4621 aataaaattt tggattagca atacctttcc atatccatta gactgagtca ttttctaccc
     4681 tccaaaagag acgacaatga aacttggtgc acgccttttt gaagttctgg cgctatggtc
     4741 cacttatgat tattgcgagc ctgcggatac ttatcttcat catgcgctta agatgaatcg
     4801 ctatatagga agttccgatc gccgtgccat acgggatttg ttttatgacc ttttgcgcaa
     4861 gcatattctt ttggaaggaa agatccatgg gtttattaaa aaaacaggta atcgccccgt
     4921 taataccctt gaattttatc gttggatgct tttggcgcac ttaaaagaaa cagatcaaaa
     4981 gggtgctctt gcaaccttca caggggaaaa atatcatttg gatacaatga cagaagttga
     5041 agaaaaactt ttagcgatcg aatccttaca accgttggat gcggcgtcca gccacaattt
     5101 aacacctttt tggtatgaaa aactccatgc acagtttgga gaaatgcttc ttgagctcat
     5161 ggcatctctg gatcaagagg caccgctcga tgtgcgggtg aatacactta aatttacgat
     5221 tgaggaagcg atggatctgc tggcaaatca taatgtcaaa gcacacgtcc ttccctattc
     5281 gcccgtggga ctgcgtattc acgacaaacg gtcttttccg aatgatcttg tccaacaagg
     5341 gctttttgag cctcaagacg aggggtcaca gattatcacg ctcatgtgtg atgtgaaacc
     5401 tggtatgcgt attgtggatt tttgtgctgg tgcgggaggt aaaacattag gtcttgccat
     5461 ggatatgaaa aataaagggc agattatcgc ctctgatatc tcgcaaaagc gtttgaaccg
     5521 tgcaaaagag cgtttcaggc ggctaaattt acaaaatatc gtggttaaaa tgatggatgc
     5581 tcagtggatt aaacgtcaca caggggaatt tgatcgcgtt cttgtggatg cgccctgttc
     5641 agggagtgga acgtggcgcc gtaatcctga gctaaaacag cgtacaaatg aaaaagacct
     5701 gcatgaactt gtgcaaaaac agcgtgctat tgtgaatgac gccagtcgcc ttgtgaaaaa
     5761 aggagggcgc ctcatttatg caacatgctc tcttttaaag gatgaaaatg atgatcagat
     5821 tgcgtggttt ttagaccaac atccagattt tcaattgatc gatttatcga atgaaatgtt
     5881 ttttaacaca cctccgtgca atacgcccac atggtcaatg acaccgctta aaaataatac
     5941 ggatggtttc ttcctcagct gtatggaaaa aaaataaaat attttatcga cattcaatcg
     6001 aaattttact ctttattaac tttcttctgt tattcctgta gatgttacta agatttatgg
     6061 agatattctg atgaaaaaaa tacttatgct gacctgctac ctgctacctg ctacctgcta
     6121 ccttagcgag tgtagtttat gcaagtgata gcgaagaaaa aagtatcaaa tttcaaaaaa
     6181 taaaactcaa ttctaagaag gattatgaga atcttaaaga gttgatcaag gaaaaatggg
     6241 aagagaaaaa agaacaagga caaaaactcc ttaaagaagc aattaaagaa acacttaagg
     6301 aacataaaat taagaaaaaa gatttcttta aggcgaatga acaatatgat acacaggaag
     6361 atgttttcct ctcaaataac gatggtagtt tcgttaggga aggtaatcct ttttataaaa
     6421 ttaaagaaaa tctttttcaa tcagagaatc aaacatccct gagtatgggg tataagtagt
     6481 gaatttatcc caaacttttc tccaggtcat taattttttg gaggagggcc ttgtcgccgt
     6541 ttgctttgag gcgcgcactt ttgacttttg ccttggcagc ggccctatcc cccttgagcg
     6601 catctgactc tgcattcgca agaagtgcat gcccctcttg accatttgcc atatacactt
     6661 tgccaagaag atgccacgca aaatcactct ccggcgcctt ttgagtaatg attgtcaagt
     6721 ggtgaatcgc ttcaggaagg tgttgctgtt gttccaccaa aagatgggcc aaagacatct
     6781 taagataaat agcctggggc ttaagactca cggcttttcg aagcgcttcg atactttcgt
     6841 ttttctggcc gatatccata agaagttgcg attttagctc aagagcataa gagttctgag
     6901 gctctagttt gagcatctga tcaatttctt caagcccctg tcgcgtttga gccgtgcgcc
     6961 catacgctac tgcgcgcgca taagagtcat tcgctgtttt gcccggaaac attttcaaaa
     7021 tacttttagg atcaagcgta aacgcaagga tctttgtgcg aatgcgctca aaggatgatt
     7081 gaaaagccgc acgcttaagc caaggaagcg ttgccgtttg cttgacatcg tgctcgacag
     7141 cctgaatacg atcttccgtt agagggtgat cgagaaaata aggactaata tcgggtgcac
     7201 cgtaagcttt ttggagtttc acaagaaaag ttaataagcc tgtggccgga atgcctgctt
     7261 ttgaaagaat tttaatcgcc tctgcatctg ctgctttttc aagattttgc atattacgca
     7321 tcacagatcc catataggcg ttttgtccgc ccgtcacacc cgccataagc ggcgctgcat
     7381 cctttgccgc aagagctgca atccctccaa gggcaaaagc aatggccgca gtcataaagg
     7441 ctttgtctgc acccacagca gcttggacag aatgaacacc ttttccgtgc gcaatttcgt
     7501 gagcaataac gcctaagaat tcttcgactg tgtcacacat gcgaaaaagt ccagcataga
     7561 taagaaattg ccctcctgag gctgaggctg cgttcacggc cgtattagca aggagataaa
     7621 tcttgggcgt aaaagttgcg cgtttccccc ccaaaggctt cagaagagta tcaaaatatt
     7681 ctttaaaaac agcttctacc tcatcatcca aaatcacact atcgtgagga atcacaggaa
     7741 gtgcgtgagc acagggattt aaacaagtac ccagtataaa aagagcagta agcgcccctt
     7801 ttatacattt gatgggatga gagatgggcg gcctcttagg attcaataag gcgtttccac
     7861 caccccttgc gctcttttcc tgaatcacct tcttgctccc ttgcttgtgt gttggccggt
     7921 gtagtcgcag cattttttgt atcacttaat gttgccacag gcgctccagc tctctcgccc
     7981 gttgacgatt gcgcgtctct gccttcagca ttttttttgt ggcgacgacg gtcaaaatgg
     8041 cgcctccgct ttttattttt tctcccatca cctacgacag actcagcagt agtctctttt
     8101 gaaactactt caggagaaga ggtttctgtt gtttgtgaaa catgaggcat ctcatccgct
     8161 atgggcgcag gagtctctga gattgatttt ttgtgagagc gggaacgctt tcgattattt
     8221 ttcttttttg gagaggtaag ctgaagggac tcatccttcc cctctccctc attttcaagg
     8281 gaatgtgctt cctttttttc ctcaatagtt ggcgctggtg ttgtcttttt ttgaagattt
     8341 gaggcacgcg tatgttgagg acgatctttt gatttttcat aagaatgtgc tttaggctca
     8401 agttttttag aaggcatcgc cgattcagat tttatttctg tcacgagctg atcttccttg
     8461 taaacctcga tatggtattc agcattttgc attgcaccat cagatttaac ggtgatagca
     8521 acatcatacg tcgtctctat atcaacaaga tctcggcgct tttcattgag aatataggta
     8581 tcaaccccca caggcacacg cacgttaagt cgtgctccct tgtgatgcat ggctgttccc
     8641 tcgatttgac gcaggacaaa aagcgccata gatgcagcag aacgaataaa tcctgaacca
     8701 cgacagtgag gacactggtg tgtgctgctt tctaaaagac taggacgcag gcgctgacgg
     8761 gagagctcta aaaggccaaa ttgaccgatt ttacccattt taatccgcgc acgatcttgt
     8821 ttaagcgact cttgaaaaat tttctcaact tgagcaatat gatttttttc actcatatcg
     8881 ataaagtcaa taacaacaag cccccccaaa tcacgcaaac gtatttgacg tgcgacttct
     8941 ttcgcggcct cgaggtttgt tttgagagcc gtatcatgaa tatgacgctc acgtgttgat
     9001 tttcctgagt tcacatcaat tgcaacaagc gcctctgttt gcgcaatcac aatcgatccc
     9061 cccgagggca gcgccactgt gggttggaac atttggtcga taaaatcttc cacatgatat
     9121 ttttggaaaa gagaaacggt tttatcttta tattgttgca cttttttcga ttgtgctgga
     9181 gacatagtct tcatcagatt tttagcggct ttatacgcct cgtcaccttc gataaaaaca
     9241 tcctcgatat cttttgtata cgcatcgcgc aaagcacgct taatcagatc accttccgca
     9301 taaataagcg caggcgcaat ggatgaaagt gttttttcac ggatatctgc ccatagacgc
     9361 aaaagatatt caaaatcttt tttaagttct gttttctttt tatccgcacc ggctgtacgt
     9421 acgatcaagc tcacgccctc tggaatatcc agactatcca gaattttgcg caggcgatta
     9481 cgatcttttt gttctgtgat tttgcgcgag atcccaccgc gccttacacc agaattcggc
     9541 atgagcacac aataacggcc cggcagagaa agatacgttg tgaccgctgc accttttcca
     9601 ccgcgttctt cttttacaac ttgaatcaga agaatttggc gccgtttaat aacctcttga
     9661 attttatagc gatagcgcaa gggacgttgg cggggtgaat cttcgtcaaa atcttcctca
     9721 gagttttcac tactaagctc aggatccccc tcaacttctt ctcctgacgc atccgcaatt
     9781 tcaacgtcac catccacagc gtctgaagga gatttaaact cctcttctgc aagatcctct
     9841 gcctcgtggc gatcccctcg cggaatatga taatagtcag gatggatttc actaaagggt
     9901 aaaaagccct ggcgattgcc gccaaattct acgaaagcag cctgaagtga aggctcaacg
     9961 cgtatgactt tagcgaggta aatgttgcct tttagttgtc ttttactaaa aatttctgtt
    10021 tcgaattctt caatgcgctg accatctgta atagccatac gcacctcttc gcgatgactc
    10081 gcgtcgataa gcattgtttt tgccatgggg catgtgtctc cgtttttatg cttttttgtg
    10141 gcgcccaata gccaagatgc gctctataat aacatgaaaa agagcgaaag ggggctttca
    10201 aaaaagcgat taataaaata gcgctgtagg gaaaaaagat aggatcgcaa tcgtacaggc
    10261 aagcagatga aattctcatg tatcccctcc ttgtgcctac gactgacgtc aaaattttac
    10321 ttatccctat gataaataaa tcacatagca aggttttttt aaagataaat ctgttgtttt
    10381 taaaagtatt ttatgctttt tccccccaaa taagggggat gtaaaaaaaa ctagtttttg
    10441 tgcgaagcag gcgccttttt atcaccgcca ccttcaggct tgtctttttc gtatatttgc
    10501 acatcaaggc ctgggaagag gcgcgtaatg cctttcgaaa caacactatc tccgacattt
    10561 agcccctcta taatctgtgt gcgctcttga taggtatgcc ccactttaac cagctgaaga
    10621 gctgcctttc catcctttac aaggtacaca aaagattgct ccggcgttgt ataaatcgcc
    10681 tctttaggca cagaaatcgc cgctggatcc tcgaaaataa taagatccac gtcaatattg
    10741 ctgccaacaa tacaatcatc gcacgcataa tcaataaaag ccggccccat gtgagactct
    10801 gggtcaatca atttttgaat acccgtcaca gggaatgatt tcccctcaat cagtgctttt
    10861 tgccccgcat tcaccttagc tacgaattcc tcaggtacat cacatgtgat caccaaatgc
    10921 ccaggcttat agacggtgac aagcacatcc cccgcattca tatgcgcacc ctcacgcacc
    10981 ttaaatacgc caacaacacc atcgaaaggc ataataaatt ggctgtcctc caggcgacgt
    11041 tttgcctcaa acgcctgttt ttccgcccgt atcgcctcca tacgtttgac ctcgacctcc
    11101 tgttttgaca cggttttcgt gttgcctaat ttttgcgcac gatcgtactg ttctttggca
    11161 atggttgcct ccatcgacgc aagctcataa tttttttccg catcagcatt ttgaatcgaa
    11221 gcaatcacat cgcctttctt cgcaacggcg ccagggagaa catgggtatc cacagcgccc
    11281 gccatttttg ccgtcaggaa agattcgaac ttaggtttga taacgccaac aagaccaatc
    11341 gaaaaagcaa agggagcttt tgtgacgttt tcaacttcaa ctattttagg tggcatctcc
    11401 ggcgctttgg agcaggctgc aagagcacaa aagaggaggc ttgtgagaaa acgcatagag
    11461 ctgcccaaaa aatttcaaga gatttgggcg aactctagca cgatttttaa cgattttcac
    11521 ctttaaaaac acacagcatc gcttaaaaaa ggcggcactg tgtcctttaa agcataaaga
    11581 gactaaaagt taagatcatt cgaaaggtta gaggtatgat tatgattagt tagaaaattt
    11641 tctgctgtat ctttgattaa atcatattta tcaaccatct ttttttcttc tggagaccac
    11701 tctttacgac ctttcttgta aatgtcctta atatcactgt ctttctcgac ggttttttta
    11761 attgcacgta gcacgtctct ggccgtactc caatacaaac gctctccttt cttataagcc
    11821 acgatatctg taatgagggt catgagccag tttttaacct gatcggtggt gtagtaaaaa
    11881 tcatcaaaca tcgtcatgta ttcgcccacc acagtcttat aggactcatc gcctttgtcg
    11941 tagtcagtat tgtaagcctt caccggatca aggtcaggtt ttttaaaaat gatattattc
    12001 ataaacttaa tgatcgtgtt gaggtttgcc tcattacaat catccagcat tgtaatcagc
    12061 tcttctttct tttgattgag ctttatcttt ttttcagaga gctttttttc ctcagtcttg
    12121 acgtttttaa tctcttttgt aatttcattt atattatcat ccttttcact ggcgtggctg
    12181 agtgttaccc cgcccgcaca caaaagaagc gacaatgcaa cggttttctt catcaaagac
    12241 ataatttttt tcctttcata gttgactatt agtttaagaa aaatattcca aacatatata
    12301 tttatatcaa aagaaagtta agaaaaagta aaaaacgtta ttttttgaaa aaatagctga
    12361 ggtacataag gggaatgcct aggatcgaga caaaaaaacg agcgacgtag gtccccagaa
    12421 tataggtaaa aatcagtgtt tgcgccgtta caggatgcgg actgaggaca acccaagcga
    12481 gggtcgaaaa aagaatatta tcgacaagtg ccgagagaag cgtcgcaagg ccagtacgca
    12541 accataaaaa acgcgtatga gtcgcatcag aaattttctt aaacacccaa atatcaagga
    12601 attgactaac gaaataagcc accaaacttg cgatcataag gcgcggcgct ggcataaata
    12661 aaagctccat cgcaccatag gtattcacgc tctccgtatt atttttgaga ggagcatgcg
    12721 cgagcgtgat gagcataaaa agtgtaaatg caatctgtgt cagaaagcta agcaccacgg
    12781 ctttgcgggc tttctccgcg ccataatgct ctgtcagcat atctgtcgcg aggtacgtca
    12841 tggcgaataa aaccgtacca agggccaccg gttcaccaat aaaagaaaat tgggcggtgt
    12901 gcaagacctg aatattcgcc gtgatataag caagattgat aaagcaaaaa agaccgcttg
    12961 caccccccac ttttaaaaaa aagagcagaa aaatagcggc aacactaaat aaaacaaagc
    13021 tcagcagctc tggcgaaagc gtgtgaaaaa acgtatgaaa aatgagctga agggatgtgt
    13081 ccataggaaa taaaaaaacg cactcagtta tgggataaag agtgcgcaaa tactttttta
    13141 aaaaggataa ttaatgagcc gcagctgctt ttttctcttc aacaagcacc ttgagctttg
    13201 caattttagg atccatatcg gctttgttcg cacgcaaaag ataagcgtcc aagcccccat
    13261 gaaactcaat actgcgaagg gcatgcgttg taaggcgcat acgaacagac tgacctaaaa
    13321 catcgctaaa gaggtttttc tcttgcaaat tcggcaaaaa gcgacgcttt gttttattat
    13381 tagcatggct tacattgtga ccatattgaa ctgtttttcc agtgaccata cagcggcgcg
    13441 tcatggcttt attccttatt gattgaaaat gcgtgtgata cttagttccc tttataatcg
    13501 tgaaaaggta tttttgtcaa gaaaaagatt cttaaaaata tgctgcaggg ggaaagattt
    13561 tttcttttga tcgctcttga aaaaaacgaa gtgagcagct ttttctcaaa ttatgagcct
    13621 aaaacattaa ccttttattc acacacctta acaaaaagtt aattttcgca c
//
LOCUS       DAIAHG010000011        13502 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_11_length_13502_cov_3.530652, whole genome shotgun sequence.
ACCESSION   DAIAHG010000011 DAIAHG010000000
VERSION     DAIAHG010000011.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 13502)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13502)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13502
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11_length_13502_cov_3.530652"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4198 a         2707 c         2909 g         3688 t
ORIGIN      
        1 agggaacaga atcttttgtt ttgatttgcg atgaccatga tgtgccaaaa aacctgaggc
       61 cggatggtgt ttgggatcac tgggtgattt ttaatctgcc taaggaaacg cgtacactcc
      121 ccaaaggtgt gaaagatctt ccagcaggag cagttgtggg gaaaaatagt tggaatcgaa
      181 acgattacgg tggcccttgt cctcctgacc gtgagcatcg ctaccaattt aaactctatg
      241 cgctcaatga aaaacttgat ctggaggggc gtgcaacaaa aaaggatgtt gaaaaagcca
      301 tggcagggca tatcttagag gaaacgcttt taatggggcg ttatgataaa aaacaaaggc
      361 gcctgaaata gtttttagga aaaattaact ggccaagtgc aaaagagtgc gaaagtgagc
      421 atgacaaata gcaatggcta aggcgtctgc agcatcgtct gtgactttgc cacagcctac
      481 taaaagcctt tgtaccatca tggcgacttg ggctttatcc gcatggccta cgcccacgac
      541 agcttttttg acacggttgg cggagtattc tccaatcaca aggttataac gtgcgggcac
      601 catcaacaca acgccgcgtg ccattcccag gcgaagtgtg ctaagcggat ttgtattcac
      661 aaatgtctcc tccacagccg cttcatcggg gtggttttct gcgatcacat gactgagctg
      721 ttcgcaaaga ctgcgcaatc ggtaggcaag agattgctga gagtttgatt taatcacgcc
      781 atgtgtaacg taaaccaagc ggttgccgat gctatcaatc acaccataac ctgttttttg
      841 aagtccggga tcaataccga taatgcgtgt cataaaaaag catagcgtga tgattttttt
      901 aaaagagtat cacaggcaat atttttttac attttattca tttgcatcga ttgtctcttt
      961 tatggtgctt ttctcttcgt ttaattccct gtatccgaac aatgtatatt cgaaggcagg
     1021 gcgttggcct acaggagaat tatcatcctc attaagcttc acatccttag acatctcatc
     1081 aataatttct acgatcgact ttggcttttg attttcctct tcctcttttt tcttctccca
     1141 cagccactca tcgttttttt cttcgtttaa taaaggaaag gtcggctttg tgttaagaag
     1201 aagttcgtta agagtgggta ttgatttttt aagatatgtt tctttcaagc attcttcgtc
     1261 attctcctgc aactttttac gcatttcttt aatttctttg atgaggatgg gggacatgaa
     1321 tacagagctc gtgacagaca agagttgatt ggactcctca tagtgttcct tgagtctgct
     1381 cattaaaaaa tctatttctt tttgtctttt tacttcgtct tcgggggttt taattgtttt
     1441 ttccagataa gactttagtt cttcctcagt cttagaaggc atggagacag aaatttttgt
     1501 ataacgaggg ggcagaaaga tcttatcctc aaagtcagag ttattagaac ccaaagacac
     1561 agaggcattg aggaaaatca aaagaaaaag aaaaacgatc atagttacaa actctcaaac
     1621 catatctttt agaagtttag aattgtaaag attaaaaaag agttaagatt gacctgcttt
     1681 gaggcgctct tttaaaagct attttaactc tttttacttc attaaagatt tttctttttc
     1741 attttcttta tgacgaggtc aatggcttgc tcaggcgtga tttctgtaaa atttatggct
     1801 tttggaatgg aggcaaaaat tttccccaca cgcacataag gtccgaattt accaatattc
     1861 acttccatag gctcgcctgt ttcaggaaaa atcccaagag gttttggaag ctgcttataa
     1921 aacatggcgt cttctagcgt gaaggtgctg agtgtgagtc cagccggtag gcctacgcgc
     1981 ttaggttttg gatctttggg ctttgcttta ctgggctttc cagactttgt gagtttaggg
     2041 ggagggggtt gatcaaactc tcccatccat tggagatacc aaccataggg acctttgcgt
     2101 aaaacaacgt cattgcctgt gagggggtct tgccctaaga tttttgattc gtcctgacgt
     2161 tgggctgttt caccctcact atctgtggcg gccgtttcca caagaggacg cgtatagctg
     2221 cattcagggt agttagagca tcctagaaac gcaccaaatt taccaagttt aaggccaagc
     2281 ttcccttcat tgcatttagg acagtgatta tcttttgccg cgccaaaaag ataggtcgca
     2341 agatcatttt gaagttggtc aataacgtgc gtaatatcca gtttcatcgc ttcttggaca
     2401 gtgtgatgga aggggcccca aaaagcatcc atgactttta gccactggcg tttgccatca
     2461 gaaatctcat ccaattcctc ttcaaggttt gctgtaaaat gatactcaac gtaggtttta
     2521 aagtaatttt ctaaaaaagt tgtaacaata cgcccacgat cattgggaat aaattgacgt
     2581 ttttccaggt gtacataatc acgatcttga agaacttgaa gaatactcgc gtaagttgac
     2641 ggacgcccaa tcccaagctc ctcaagtttt ttaacaagac tcgcctcgcc atagcgggga
     2701 gggggctctg taaaatgttg gcgcggtatt attttatcaa gattcatcgg atctttttca
     2761 cgcacaggtg gaagaagttt ttcctcatct tccgaagttt tagtgttgtc cgcaccatca
     2821 tctgtgcctt cttgatatac gcgcaaaaaa ccatcaaaaa caagcgtcga gcctgtcgct
     2881 ttaaatgcgt gctgatgatt atccgagtcg atgacaaggc tcacttggtc aaaaacagcc
     2941 tgcgtcattt gacaggcaag cgtgcgcttc caaatcaaat cataaagttt atattgagct
     3001 tcatcaagat agggctttag ggactcaggt gtacgggtga tatctgtggg acgtacagcc
     3061 tcatgggctt cttgagcatt tttggctttg cttttgtaaa cgagaggcgc ctgtgggaga
     3121 tatttaccgc catagttttt ggtaatatag tgacgcatat gctctacagc ttcggcggat
     3181 aattgcacac catctgtacg catataagta ataagacctg tgctctctcc cccaatagaa
     3241 atgccctcat aaagtttttg agcaatttgc atagtgcgac tggcgctaaa cccgagtttg
     3301 cgcgaggctt cttgttggag tgtggaggta ataaaagggg ctgtgggatt gcgcttaacc
     3361 tgctttttat caatggattg gatattgtaa tgatgttttt gaagagtctc gacagccgtt
     3421 gttgccaaaa gttcgctatt aatcgaaaac ttctcgagtt tttttccact gagatgcgtc
     3481 aattttgcgc ttaagggtaa attttctggt gtgtgaaagt gtcctgtaat cgaccaatac
     3541 tcttcgcgtt taaacgcttc aatctcgatt tcccggttca caataagccg taaggcgaca
     3601 gattggacgc gtccggctga gcgagatcca gggagttttc gccaaagtac gggagaaata
     3661 ttaaagccca caaggtaatc taaggcacgg cgcgccatat acgcatccac gagatcttgg
     3721 tttacatcac gggggttttt aagcgctgct tggacagcgg tctttgtgat ttcattaaaa
     3781 acaatgcgcg caatgtgttt gcctttgagg gcttttttgt cctgaaggac ttgtaaaatg
     3841 tgccaggaaa tggcttctcc ttcgcgatca gggtcggttg cgagatagac tgtatcaacg
     3901 tttttgactt cgcgcaaaat cgcatcaata tactttttag agcgtgcatc gatctcccac
     3961 agcattttaa atgcgtggtc agggtccaca gagccatttt tagagggtag atctctcacg
     4021 tgaccatagc ttgcaataac actaaaatcc ttgccgaggt aacggttaat agtttttgct
     4081 tttgcagggg actcaacgat aacaagactt ggcataagaa aatgtaaaaa cctcttttgt
     4141 cgttcaaata gccataagag gttgcgacct cttaacacat ttcattctgt ctgtgttgta
     4201 agccaatagc aagctttcca ttcagttgta aagaaaatag tgagggggtg ttgatgatta
     4261 taaaaaaacc actccgaaga gtggctttga gttttttagt attttttacc ggttggccat
     4321 tattttgcaa gcattgcaag caagagaagg gcgacgatgt tgataatttt gatcatggga
     4381 ttaatcgcag gacccgctgt atctttataa ggatctccta cagtatcacc tgtcacagcg
     4441 gctttatgag cctcggagcc tttgccgcca tgatggccat cttcgatgta tttttttgca
     4501 ttatcccagg ccccaccacc agacgtcatc gaaatagcaa cgaaaatacc tgtaacgatc
     4561 gttcctgtaa gcattgcacc aagtgctgag aatgcagagc tttgacccgc aatggcattc
     4621 actgcaaaat aaagagcaat cggcgctaca atgggaagga gggatggagc aatcatttct
     4681 ttgatcgccg cacgcgtcaa aaggtcaaca gcgcgggaat aatcaggttt tcctgttccc
     4741 tccataatgc ctttaatctc gcggaactgg cgacggactt cgatgaccac agccgctgct
     4801 gcacgcccca cagccttcat acccatggaa ccaaaaagat aaggcaaaag accaccaagg
     4861 aaaagtccaa tcagcacaaa aggatctttg agcgaaaatt caacattaag gtttggaaaa
     4921 taatgtttaa gatcttctgt gtacgctgca aaaagaacaa gtgctgcaag ggcggcagag
     4981 ccaatagcat atccttttgt cacagccttt gttgtgttgc ccaccgcatc aagcgcatct
     5041 gtaataacgc ggacttcttt gggcaaattc gccatttctg cgataccgcc agcattatct
     5101 gtgacagggc cgtaagcatc gagtgctacg acgattcctg caagggcaag cattgttgtc
     5161 gccgcaatac cgacaccata aagacctgca ttcatatacg cgataatgat acctgcacag
     5221 atgacaatca cagggagtgc tgtggcttcc atggacacag caagaccttg aataatattt
     5281 gttgcatgac ctgttgtaga ggcttcagca acagacttga cagggcgaaa atgcgtggat
     5341 gtataaaact cagtaatcca tacaagaagg cctgtcacag caagacctgt aatcgcacag
     5401 ataaaaagat tcatcgatga aaatgtcata ttttgaatct gcatcatggg taaattcatg
     5461 gcatccgtca aaaggaaaat ggcacctgtt gagacaacag aagccgcgat aaagccctta
     5521 taaagcgctt tcataatatt ttgactgttg ccgaggcgta caaaaaatgt gccaataatt
     5581 gaaccaacga tacagacacc gctgataaga aggggataga gcataagggc cgattgaata
     5641 ggtcctgtaa agaaaattgc tgcaagcaac atacttgcca caatagtcac agcatacgtt
     5701 tcaaaaaggt ctgcagccat acccgcacag tcgcccacgt tgtcgcccac gttatccgca
     5761 atcacagcag ggttgcgagg atcatcttcg gggatgcctg cctcgatttt tcctacaagg
     5821 tccgcaccaa cgtcagcacc ttttgtaaaa ataccgccac caaggcgtgc aaaaatagaa
     5881 atgagtgatg caccaaagct aagggctacg agggcttcaa ggatttggcg tggttcatga
     5941 tgcgcagtga caagataaaa ataataaccg ctgacaccgg ctaaaccaag gcttacgaca
     6001 agcatacctg tgatggcacc cgatttaaat gcgatatcaa gagcgcgccc aaggccactg
     6061 cgtgccgctt ctgctgtgcg cacattcgca cggacagaaa catacatacc gatatagcca
     6121 gcaacgccag aaagtactgc gccaatgcaa aaaccaagcg caacaagtgg cgataaaagc
     6181 cacgccaaaa gacctgcaat gacgacacca accatggcaa tggttgagta ttgacgattt
     6241 aaataggcgc tagccccctc ttggatagcg gctgcgatac gttgcatatc agcattgccg
     6301 gttgccgccg aaaaaacctg gcgacttgca aaaagcgcat agagaagtcc taaaatagca
     6361 cttccaataa tgagaaaaac aggatccaca aagacctcct tgtaagagtt acacacaagc
     6421 acatcataaa atgatgcttc tttgcgtgac aggatacgtt aaattggcaa aatttgcaaa
     6481 tttccttttg cttttgtcct aaaacggatc acttaatctt tctttaatag gcgtctattc
     6541 aagtgaatga ttgtaaatcg tggtgtctta tttatttacc accaaccgag aaaacgccac
     6601 caaaggccgc caaggccaag ccatataata aggctttgga tgctgacgaa aaaaccaagt
     6661 gtccaccatg tcgccacagg aacatagcct gttccaaaca aaaccacagc ggaagatgat
     6721 ccatagtggg tcatgtgaga aaagaggctg ctgcaataac caaaaagaag ggctgccacg
     6781 aggggaggtg tgccaattgc aatcgcgact gttagaaatg caccgtacat agcgcttaca
     6841 tgggcggtat tgcttgcaaa gaaatagtga ctatagaaat aaacagcgac gagaaataaa
     6901 aaggcccaat gccactcaaa ttggcttacg taagcgctca tgctgtgact aaaccatgca
     6961 acaaaaccat actcgctgag gtattttgcc atggtgacga ggattgcaaa ccaaatcagt
     7021 gtgtgccatg cgtctttttc ttttaaaatg tcctcaaagc ttaaaacgcc tgtgagaagt
     7081 aaaatggaaa gacccataag cgctgttgtt gtgctgctga caccgatgct tttaccataa
     7141 atccaaaagc caagcataag ggcaaacgtc ccaatcatga tccactcgtt ttttttaatg
     7201 gggcccattt ctgttaattt ttctttggca agacttaccg catgaggaag aattttaagc
     7261 tcaggtggat aaataaaata aagcatgagg ggcatgagca agatgcttgc aatgcctggg
     7321 acaatagcac ctgtgaacca gttgccccac gtaatcatgg cgccagagct tgcggcaatt
     7381 ttctgtacca tggggttcgc cgccatggct gttaaaaaca ttgcacttgt aataaggttt
     7441 ccatgatagc tgacctgaac gagaaaactt ccgatttttc gctctgtgcc atcatgcggt
     7501 ctactcccca aagactcggc catggatttg agaataggat aaataatgcc acctgcacgg
     7561 gcagagttgc ttgggataag cggcgcaata aaaagttctg taaacacaat gccatagcca
     7621 aggccaagac tatattttcc taaaaaccga acaaagatat aggcaatgcg tgtgccaagc
     7681 tggctattaa taaaccctct cgcaatgaaa aagacataaa ccacaagcca aatcacatca
     7741 tcgccgtagc ctgacaaacc ctgtgtgcca agatcaagcg ttcctgtgag ggcggcgact
     7801 acaaggctga gcattgcaag ggcgcccata ggcatggctt taaggataat ccctaaaatc
     7861 gttgccacaa aaatagcgaa aaggtgccaa cctcttggat caaggcctac gggaacggga
     7921 aggttgtaca agaccgcccc aaggaggaca gggattaaaa gacgcgtgag cataaaaact
     7981 ttggtattga cgaaaatgat atccattgta gggaaatttg cgcaccttta ccaagaattt
     8041 ttcacaaaaa tcttttaaaa actatttttt ccttcattct taacgattta ttcatttttt
     8101 acttttacca tgagtgtatg gaacggtatt ttgcgcatag actgcgtgaa atagaggtga
     8161 ataagcatat aattcttgaa aataaagggc gtgtgtacta aaaatagccc atatttcaca
     8221 atgaattata atatttattt aaaattttat taatagaggg ctttaataat gaaacactat
     8281 ttaacacttt tatcactcct catggcaagc acagccatga tcacaacagg cacgtcaaaa
     8341 acattcgctt ctgatattaa ggaagatgaa gatatagact ggaagctgaa ggagatcgct
     8401 acagacgatg aggatgatgt tgaggagaat gttattcccg acgaaagtcc gtatgattat
     8461 atcacttatc cttttaatgg ggatgactac tatattatcg ctgtggatga aaataaaata
     8521 aaaaaacaaa gggaatctgg tggaatgagt tcaatggagg gcttttataa agaagttctc
     8581 aaggaaaaaa caaaaactat gggagctctc cttaaaggag cttataagga cgaggatggg
     8641 aatattcttt cctccgaaaa ggtctttgca aataaatttt tagattttgt ggatgctttt
     8701 cctaaggatc caaatctcaa caatattatt aatgtgcttc ccaatgatat tttgaatgat
     8761 gaattggtta aaaaagaaat aaataattta aaagaagaga aaagtaaaaa attcaacatc
     8821 gtcaataaaa ataaaaaaga agaaaacgaa ataaaagatt tttcaaaaga aataaataat
     8881 cttaaggaag aaaaagagaa taaaaacctg aatatcaatg cggaaaaggc tctccattat
     8941 attaattatg aggggtataa tgttcttttg aaaaataatt gggagaatat taatgataaa
     9001 gaggttgtaa acttcctcaa aaaatacaaa cccatgacac agtatacaga ccctcagtac
     9061 aaggataacg cgttgtttaa aacaggggga aaatatcaag gctattataa caaaccagaa
     9121 aattttgagc gtgaaatgct caaccttttt aaaaaatctt taaaggatgg tgaagataaa
     9181 gacattcttc tgaaacttat tccagatgat atgaaaaaag atattgagga aaagattgaa
     9241 aaagagaaaa acgataaaaa gattcaaaat gagcgtgagc tgcgtttaaa gacagtgaac
     9301 gacgtggcga aaaaactaga aaaggataat gatctctatg tcatcattac agatgaactg
     9361 ggcgctccct atcgcattct tgtgaataaa tcaaaacttg gcacccagga agataaattc
     9421 gaggagaata aatcaatctt caagtttagt aaaagttggg gcgaaggaca tagtcttgca
     9481 gactatgttt ggcaaacctt taatttcctt gatgcagaaa aagaaggaaa acaaaatcag
     9541 atgactcaat ctttgtttgg aaatctaata aatcattcaa tcccagaaac tattctcaat
     9601 gcttattatg ggatggctgg cataaatgcc ggtaatgaga atgtaatcga gaaaattatt
     9661 gacgagaatg taatcgagaa aaaagaagaa ataaagatcg ataatgtaat cgagaataaa
     9721 gacgaaataa aagaagagaa aaacgagaaa aaatccacgc ctattgataa tattttgaat
     9781 gagaaaatca gcaacaggga taaagccctc aaggtgattg actatgccgt aagccagctc
     9841 gacagtgatt tctggcctta tgcgtttggt gagaaaatga gggaacttgg cgtgaatatc
     9901 atcggttgga atgaacttct ggatggtgcc cttgataagg aacttgatga ctttgaatat
     9961 aagcctctga gggcgaaact tgcggtgaat aatctgaaaa ataagattat tcaaaacaag
    10021 ggttataata agattttaac gttcccaggc tataactaac cgtcacaggc ggacctcaat
    10081 cagggtgaga tcatcgttca atttgatgtc acccagcatg tccaggtggc cacggagcgt
    10141 ggctaccaaa ttttttccct ggagctcgtc gatgaaggag gggagaaatt ccggcagcaa
    10201 acacacgccg ggcagggatt tgggctccca caaaaggtcg ctgtacagca cgatggaact
    10261 gccgcttggc gtctccatgg cgcgctcggg ataggccgca ctggcgctca cgcccatggg
    10321 caaaccggac ccatccccca cccaaaaagc ccgcttttcc ggggtataga gaatcggatc
    10381 aggtgcgctc gcaccgacgt aacgaaaggt ttttttctcc gtgttgataa tccccatcaa
    10441 aagcgtcgca aactgcccca cattcaccat ttcatggaac ctcgcattta aaagactgag
    10501 aaatcctggg ggtgtatggg ccgtgttttt atattcttgg acaagcgtgt gaatacgaaa
    10561 ggtattaagg gctgcttgga tccccttacc tgaatagtca caaatccaaa cacccaattg
    10621 atggtcgtca atttcaatga gcccccaaag gtcgccgctc aaaaaacggg agggctcaaa
    10681 aatataatca attttgacat tatagcgctc ttcaatggcc gcaatatttt ccggactggg
    10741 caggagggat ttttgaagat taaacgcctg cttaatatca ctttgagagg tagtgtaata
    10801 accctcgagt tgtttgatca tatgcattgt gacaagctga acctttacgc gggataaaag
    10861 ctcaagcctg tgaataggct tgcttaaaac atcattcgcg ccggatgacc atgcttcttg
    10921 tttttcttcg cttgtggtga gcgccgtttg cacaataatt ggaatatggc ggaaggcgtc
    10981 acttttttta gaaattttaa taaattcaat cccccccatg acaggcatca gaagatcaca
    11041 gataataagg tcagggagaa atgtttccat tttttctaag gcatcttgac cgttttcagc
    11101 catttcaata tgggtatacc cttctccctc aaggtatgtc aaaataagct cgcggttgag
    11161 ggctgtatca tccacgatga ggatattgga attaaaaata gtggcgctat taggattgag
    11221 cgactgcttg ataaacattg tctgcctctc caaaattaag cgtcaaagtg tatgttgatt
    11281 tgtcgtaatc catctggcta aaaagacgta gaatatcact tcctcgaata tgaagagagg
    11341 gcgtactttg aaaggattca aaattaaaat tctcatcata ggggttaatg attttaatgc
    11401 taaaacaagg ctcgatctgc acagcgacac agagagtttt cctttggctt tgagtctcaa
    11461 tgagcttttt ttcaatatat ttatgaaaat cgagaatatc ttctggccgt ttgaccttta
    11521 aaagccccca taaaagagag ttagagaggg cttcgtgaaa ggctgtcagt gccataggcg
    11581 aaagatattt tttacagtgc ttcgcaaggt aataattcac aatctctaca ataggaaaag
    11641 tgtaatagca cctgggagat aaatggagga caaaaacgtg attctcatca aacgtataat
    11701 gagggtatgc gtagtacgat tggaagaaaa aagaaattcc tttatcgata tcacgataaa
    11761 aatcggtgag agttatgtca ccgtgataca tgattttttt taaagctttt ttcacaaaaa
    11821 taatctcatt ctattgtaaa gaggctttca aaatttccaa gtgtgagcat ttttttcact
    11881 tgtccttggg agttttttaa aacgacatgg ccatttttgg cggttacttt gtcgcgaacg
    11941 ataagcagca ttccaaggcc tgcggaatca atgaattcaa gttcttttag atctaaaaga
    12001 caggattttg ggctaaattg attgagataa tcgctaattt ctcgaaaaac agaataatca
    12061 gaaaaagtga gtctccctcg aaggtcaatc tccatttgag ggcttgtgct tttgagctcg
    12121 taaatcatgg gtcccttgcc tttatcgtga caatgatcat ttcagttctt atctttgagt
    12181 gtcgcttaaa aaccttaaga aactcttaaa aacatttaaa aaaataatta tattttttga
    12241 attttagggt aagagaaaag agatagaatc agaagaatat tgagtcctat taaaaaagga
    12301 aaaaggttcg tgagcgacat ccacccatta tcaaacataa aacacaaaag aaaacagatg
    12361 ataaaattta tatgccctag agaaaaaacc tgatgcagca caatttccac tcacttcgtg
    12421 atcctcttat ccaccatttg acgataaaaa agttaataat ttcctaagaa aaatcttttt
    12481 agatgcacat aaaaaaaagc caaaaattaa ttttctttaa cgattgtgtg ataacctttt
    12541 attaggaact ctttgaaaaa gaagctatag atgcaatgtt ctttggccct aagtattgga
    12601 atttttttga ttgtggcgcc tgctgaggca cgttggcacc atcacgatca ccgtcattgg
    12661 ggatggggtg ggcctgtcat tattgggcct gatccctttt ggccgccggt ccaagaagag
    12721 gtcattatca caccacctcc tccgccagtg ttaacacccc caccagcgcc tccagttgtg
    12781 gttaaagaac gtgtgattat caaaaagccc aaaaaactta aaaaaatatt ggattgcaaa
    12841 ccctatcgaa agtgtcttga aaagggtgtg gatattccaa aatgcaaagc aacttatcca
    12901 aagtgcgtag gcaaataaaa tgcataaagg ctaaagccta agaaacagaa catataaaaa
    12961 gtaacgaagg gggcatgctt ttcttttgtt gcataatgat aaaggcaaag gctgctcccc
    13021 aagcatataa aaaaaagagg gatcatcgat accgcaatta aaagaggtgt tgcgcacact
    13081 aaaaaaagat cagctgtggc tatccatctt tttttaagac gatactgaag accgtagatc
    13141 attaaaaaaa acgcacaaca gacaatactt tctttccaag ctggattttt ccaccaaagg
    13201 ataacgctta taacaattaa aaaatccaca agccgtatcc tacgagaggt gtagtcttcc
    13261 caagcgatgc ggcttgcaaa aaaaagaaaa agagggagga aaagagcagt cacgctcttt
    13321 ttcctttgag ggaattattc ccagtcatag actttttatt ccaaaatatt cgtccaaaca
    13381 ttctttttga catgattttt atttttcttt gcgttatttt ttaatttttt attcttcttt
    13441 gttttttaaa ttataggagt ttgtccattt cctgctgatg ttagtagtgg aagagcagtc
    13501 gt
//
LOCUS       DAIAHG010000012        12863 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_12_length_12863_cov_3.147974, whole genome shotgun sequence.
ACCESSION   DAIAHG010000012 DAIAHG010000000
VERSION     DAIAHG010000012.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 12863)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12863)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12863
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12_length_12863_cov_3.147974"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3617 a         2608 c         2871 g         3767 t
ORIGIN      
        1 gtttttatgc tgagagcgct catcttgaca cgtcacaaca gtatttgtgg gaagatgggt
       61 aatacgcacg gccgaatctg ttgtattcac gtgctgtccg ccggcacctg acgcacggta
      121 cacatcaatc aaaaggtctt tctcttcgat atgaatatcc acgtcttcgg cttcaggaag
      181 cacagctaca gttgccgcag aagtgtgaat gcggccgctg gcctctgttt ctggcacacg
      241 ctgcacacgg tgcacaccgg attcatattt aagacgggcg aaaacaccct tgcccataat
      301 cgacatgcta gcctctttaa cgccgcctac acccgtttca ctgaggttta aaatatcgac
      361 tttccatccc ttaagcgctg cataccgttg atacatgcga aacaaatctg cggcaaaaag
      421 acccgcttcg tcaccgcctg tgcctgcacg gacttctaaa attgcattgc gctcgtcatc
      481 catgtctttg ggcaaaagga gaactttaat attgagctcg agggttggca ttttttcttt
      541 aaggtcaaga atttcttgct gggcaaggtc tttaaattca cggtccgttg ttgaatcttt
      601 gagcatctcc tcaaggtcgg ccagctgttt ttctgcctca aaaagctcta agattgcttc
      661 tttcacaggc gttaaatccg agtattcctt ggagagtttt gcgaattcag cagggtcaag
      721 gccctgtgca gacatgagtg tgctgaggtc attgtgccga tcaacaacgc ggctgagttt
      781 agctcgaaaa gacataggtg tatcctaaaa atttttgaaa aaaagagtgt tcttggcggc
      841 caaagggcac tcgtccttaa aataggtgaa tttaagggat aaagctatct ttaaagtgtt
      901 ttaaggggga tttgactgat ttttttggat tgagggagca catacgtgct gcgctccctt
      961 tttttaggta aatactaatc cttatcttca tcagaaaaat attcgtactt ttcaaaaaga
     1021 ttttctattg ctgagcggag attctcgctc gttgactcat catcataagt tttctttaaa
     1081 gactggaaga tcttatcata gcatttttgc gctattttct gagatttttc atccaacaag
     1141 atcgtgtctt tgagcttgtc ttggagctca tagattgatt taaaaatctc aaaatgatac
     1201 tgatgagcaa ccctaccaaa gacgcgatca agtgtatctc tgtcattgtt tgttaaattc
     1261 ttcttatcaa gatcaagact ctttaaatcg cgagtaagca tgatgagctg tagcacgggg
     1321 ggcatattat tagggctatt tgtgaggcgt tcaatttcct catctttttc tttgagtttt
     1381 ttgtgaagct tggaaatatc ctgcaaaagc ccgctgtgct tctctcccca aatgtcgttt
     1441 tctttttcaa gcttttttat gttctcctcg agttttttga ttttctcatc aaactcactc
     1501 ttaagctttt gaattttttc aatatccctt ctttcactat tgatctgatt catggcctga
     1561 ttcagttcat gagtctcctc ctcactcgcc ctcacaggag aaaggtcaaa tgctaccccc
     1621 cccccataga tattaatgac attagtgtta aaatcttttt cataaaatta ctccatttta
     1681 ttaaagttaa tgattgatca cccatctcta tagataacag agaaaagtaa agaaagggtt
     1741 aaccttgcgc tctatattag aaaaaaagtg catgcggccc ctcctcacaa tttgattttc
     1801 tattcattgt gcatttgtag gatggacatg tcagagggaa ggggcgctgt gaaggtgagg
     1861 gtttctcccg agcgggggtg gataaaacgt atttctgttg catgaagggc ttgacgggga
     1921 aagtgtaaaa tcacctctgg ccaggattga gataaggttt ttttgccata aagagggtcg
     1981 ccaatgaggg ggtgaccgat actttgcata tgcacacgaa tttgatgggt gcggccagtc
     2041 aaaagagtgc agcggacttt ggcgtaactg aggttttttt tctcatcgac gacccaactt
     2101 tgaagtgttt tataaagagt aatcgcattt tttccataag gttccttcac aacggtcatt
     2161 ttttgccggt gtgtggggtg acggcctatg ggtttatcaa tgcgtccctc ccttggaaca
     2221 ggtatgccaa aaacaaaagc aatatatgtg cgcgacaagg agcgatctgc aaattgctca
     2281 ctcagctttt gatgcgcata atcattttta gcaatgacca taagccctgt tgtattctta
     2341 tctaggcggt gcacaatccc cgggcgctga accccgccga tgcctgataa tgaatcacca
     2401 aagtggtgga gcaatccatt cacaagggta tcatcctctg tgcctgcgcc cggatgaacc
     2461 gtgaggtctg cttgtttatt aatgacaagg acatcgtcat cctcataaag aatatcaagc
     2521 gcgatatctt ttgggataat gtgagagggt ttggggtcat ccagtgtcag tttatagatt
     2581 ccctgtgtgg cttttatgtt acttttctgg acaatatgat cattatgcaa aaggcttttt
     2641 tgtcgaatca ggtcttgaaa gcgattgcgt gtgagttctg gacaaagctc ttgaaagagt
     2701 ggcaaaaggc ctttatccaa gcgttctggg gtgtcatgat tgaggataac ggttaaaatt
     2761 ctcacaggcg ttaaagattt attaaggatt ttcttgtacc tttttagcat aggttggaag
     2821 atttgtgtga aaacagctct tccatttgga ttaaaagatg ttaaaatttt attgatgtga
     2881 tttaacacta actgaaggat taaattctta tgaaaaaagt actttttaca ctcgcagcgc
     2941 ttttgacaag cgcaactctt gtaacacccg tattcgctac tcaggaagaa gttccagctg
     3001 atcagcctac atccgagcct gtcgcaataa atgcgcctgt tcatcacaaa aacaaacagg
     3061 aaaagatgac aaaagctgac gttgaaaaag cgtggaacga ggctgttgca aaaatgacag
     3121 aggcaatgaa gcatgtgaca gatgaagacc aaacatgcct taggaccatc catcaagcgg
     3181 cgatgaacgt tgatggcgct gctaagaaac ttgaggctgc tgctaaaaag cttgaggctg
     3241 ctaaagcgaa aaaagaaaaa aaagaagaac ctaaaaaaga tgaaaaagct gcagaaggcg
     3301 ctaaggctga agagaaaaaa gaagagccta aggctgaaga agcaaagcct gaggaaaaga
     3361 aataagtttc tcttcgcttt caaaacaatt aaaaaggata ctatatcaat ggtatccttt
     3421 tttcattttc aagtatgaca gatctttcct ttcacccttc tattttgcgt gcttatgata
     3481 tccgcggaac ctttggtgaa aatctttttc ctcaagatgc cactctttta gggcaaaaaa
     3541 tcgcagcgta ttttaagcgg tatcatccct caaagacgtg taccatgggg attgtgcgcg
     3601 acggtcgcca tagttcaccc gcgcttcaca aagtccttat cgaatcgctt ctttgtcaag
     3661 gtattcacgt tctagatttt ggtgtaggcc cgtcacctct tggctattac gcctcgtttt
     3721 ttgagaaaat agaaggactt gttgtaatta cagcgtccca caatcctaag caggataatg
     3781 gttttaaaat tcttctcctt gggcaaaata tatgtggtga tgcattgcat gcgtttgcat
     3841 cactgccggc gtgcccttct ccagaggaaa aaggacacta tcatgcaaaa aatatattcg
     3901 aagattatat acagcatacc cgtcaggcac ttgcgaaaac ggctacaaag ttgcgtgtgg
     3961 cgtgggattt tggtcatggg gccatgtctg tgcttgcacc ttatattaaa aaatatatcc
     4021 caggcgagca tttttttctt tgtgaaacac tcaatggcga ttttccaagt catgaaccgg
     4081 atccaacagt ggagaaaaat cttgctcttt tgaaagagtg tgttgtgagt aaggggtgtg
     4141 cgattggttt tgcgtttgat ggggatggtg atcgtttggg cgttgtggat cacacaggag
     4201 cgatgattgc aggagatcgt ttagtgactc ttttaggggc gcatttttta aaaaatcaac
     4261 cagggacaac ggttcttgct gatattaaat ccagtatgat ttttaaggaa gttattgaaa
     4321 aaaatggggg tgtgcctctt ttgacccgtt cgggccattc tttcattaaa acaaaaatga
     4381 aagaaacagg tgcccttttt gcgggggaga tgagtgggca tctttttttt gcagatgaaa
     4441 ataaaggcta tgatgatggt ctctatgcgg ccttgcgcgt tttacgaatg cttgatgaaa
     4501 tgcaaatgtc actgcatgag cttaataatg ctctgccttc ctatgatgca acgccagaga
     4561 ttaaattatc tgttgaggtg ggcgagaatt ttgcaactgt tgagcgtgtt aaaaaagatc
     4621 ttatacatca acaaaaatct tttagtgagg aagatggtgt gcgttttgaa acggacaaag
     4681 gttggtggct tattcgggcg tcaaatacgg gaaatcacct tattgtacgt atggaaggaa
     4741 gaacgcaaga tgatcttgaa gtgatgaaag cggattgcaa gtctatttta aaaaaagctt
     4801 taaacggtac cataacgttg gaattttagt ttttttatgg cttgctaact ctattgtgtt
     4861 tcaatctttt tctttgaaaa aaaagataaa aatcctaagc agcatcctca agacctttca
     4921 caccatcaac cgcaaggaga cgggagcgca catctgctgt gacatgatag cgctgaggca
     4981 aataaatctg cacaggtgtg gggatgttgt cgagctcaat gtgaatcaca aggcgtgttt
     5041 gcccttggcc gcagccggat aaaatagcct gcatcatcgg cacagaagtt gacgtatcca
     5101 cgcgaagacg aagactgtgt gtgagatctt gaacaatatc ctcaagagag ctcacactgt
     5161 tcgcaagcag acgaaagcct tccccctctt gtctcaacgt tgcagtaata aaaagggctt
     5221 tgccaggaat aagaagatcc cgtgcttttg tatagttctc agaaaaaagg gcaacctcga
     5281 aaacaccttg ctgatcagac atttgaacaa aggcgtactt gttgccattt ttgcttgtgc
     5341 gctctgtttt tgtaataata acaccgacga gctttatagc cgtattatca ctgagatttg
     5401 agagtttatc cgatgttatc gcacccaatt ttctaaggtt agtataaatt tgaaggggat
     5461 gggaggagag aaaaaaagaa agagcattaa actcatgggt aagtttttca agggggcccc
     5521 attttttcac aggctccatc gcaaaggatt gatgagtcgg agcggcacta aaaagggatt
     5581 gttgtgaact tgtggcgcct tgtttacgag aagctgcaaa acgcagcatc gcctctgcat
     5641 ggtggtagag catggcgcga ttggtttcca aagcatcgaa acagcctgca gcaatcagat
     5701 tctcaagctg acgtttgttc ataatgctat ggtcgaggcg ttggacaaaa tcttcgacac
     5761 ttttataaag accattcaac tcgcgttcac gggtgaggga ctccatagcg ctggtaccaa
     5821 catttttaag agcagctaaa gcatagcgca cgcctccctt gtaggggctt gtggtctctt
     5881 ccacacaaaa ctctgcttga gaaaaattaa tatccggagg cagaagttga atctgaagac
     5941 gctccatgtc ttgacgaaac gcattaagtt tgtcggtatt atgcatatca tacgtcatgg
     6001 aagcggcata aaattcgctt ggatagtttg ctttgagata ggccgtttga tacgcaagaa
     6061 gtgcgtaagg cgccgagtga gatttattaa aaccatagcc tgcaaacttt gccatttgat
     6121 cgaaaatttg gcttgcaata tcgggagcga caccctgctc gatcgcacct tttgtaaaga
     6181 ttgcgcgctg tgcatccatt tctgatttta tttttttacc catggcacgg cgaagcatat
     6241 cagcagcgcc aagactataa ccgccaagtt tttgagcaat atgcatgacc tgttcttgat
     6301 acaccatgac cccataagta tcttttaaaa tcggctcaag ggcggggtga agatacgtga
     6361 cggcctcttc accgtgttta cacgcgacgt aacgcggaat atcatccatg gggcctggac
     6421 gaaaaagagc aacaagagca ataatatctt caaagcggtc aggtttaagt ttacgcacaa
     6481 cctcacgcat gcccgagctt tcaagttgaa acacacccat tgtttcaaca cgttgcaaaa
     6541 gctcaaaggt tttttgatca tcaaggggaa tttctgcagg cgtaatgttt atgctttttt
     6601 cgcgaataag tgcacagcac ttttcaatca cagtcagggt tttaaggcct aaaaagtcaa
     6661 atttgacaag accagccagt tccacatatt tcatattaaa ttgtgtggcg ggcacacctt
     6721 tgggctcatc gagataaagg gggacaagct cataaagcgg tcgatcaccg atgaccacac
     6781 ctgccgcatg tttggatgcg tggcggtaaa gcccttcaag ttgtgtgccc atatcaatca
     6841 gttcgcgcac cgtttcatca tttttacggg cttcctcaag cgcaggctcg agggcaagtg
     6901 cttgttcaag agtgacagga ttagcggggt tattggggat cagtttgcta attttatcga
     6961 cttgattata cggcatttgc agcacacggc ccacatcacg caacacggca cgggcctgga
     7021 gttttccaaa ggtaataatg tgtgcgacgc gatcatgacc gtatttctcg cggacataat
     7081 caatcacttc atcgcggcga tcttgacaaa aatcaatatc aaagtcaggc atggagacac
     7141 gctctgggtt aagaaaacgc tcaaagataa gaccaaagcg aatgggatcc atgtctgtaa
     7201 tcgtcagcgc ccacgcgaca agggatcctg cacctgagcc gcgccctgga cctacaggaa
     7261 tagagcggat tttcgcaaat tgaataaaat cagcgacgat gagaaaatat ccggggaagc
     7321 ccatcgcttc aataaccttg agttcaaaat ccaggcgttc ttgatagtga gggggaatct
     7381 ctggtaaatc tttaagacgt gcgcgcaatc cctcatatgc ttggcgttga agttcctccg
     7441 cctcactgcg gccttcgcta cttttataag aaggtaaaat aggtttgcgg ccactcggca
     7501 taaagtgaca gcgctttgca atcgccacag tattataaag ggcttctgga agatctgaaa
     7561 aaagcgcatg catgtctttt gagtttttaa aaaagtgctc aggagttacc ttgcggcgat
     7621 cattttctgt gacataccga ccatcggcaa tgcataaaag agcatcgtgg gccttgtgca
     7681 ttgtcttttc ggaaaaaaac acatcatttg ttgcaacaat aggaatatgg tgtgtgtccg
     7741 ctaattttaa aagctccgtc tcaacctctt cctcttcaga atcaaaaatg tcctcggtgt
     7801 tatggcgttg aatctcgata taaaagcggt caccaaaaag atttttataa aaagtgatct
     7861 cctccaaagc gtcatcgttg cgtttttggg acagcaatcg atacaaagat ccacgctttc
     7921 caccattcag acacaaaagg ccctgcgcat gttctaaaag atcattgtga tgaatttgcg
     7981 gcgggtacgt tggatcaatc gtaaggtagc tttgactaat gagttttaaa agattgagat
     8041 atccctcctc gttttgacac aataaaatca tgtgatcggg atgtgatttg gccttggttt
     8101 catccttttg aaaagtcgct gtgacattca cctggcatcc aataatcggc tgtacgcccg
     8161 cgtcggcgca cgccatagca aattcaaggg caccaaaaag attatgcgtg tccgtgatgg
     8221 cgacggctgg catggcattg tcaaggcaaa gttttggaat cgctttgatc atcaaagccc
     8281 cctctgcaag ggaataggcc gagtggaggc gcaaatgaat aaacggaagg gtcaaaggag
     8341 aatcgttttt agagaataca aaaaatctat atccttactt ttacaggaaa aatccttaag
     8401 atgctaggta tgtagaatgc aaaataaaat ttgtttttag attttgaatg gtctatataa
     8461 atattctaaa aaatattttt aaatgagatt ttaatgatta aatacatttt tattttgagc
     8521 gtttttacaa gtgttgcttt cggaagtgag tcaaatcttg agaaagaaca tcctcttaaa
     8581 cgtaaatatg accagataga agaatgtaaa tatgaaggat taacagataa tcaaaaaaaa
     8641 attcaagaaa aaattgaaaa gcttcttaag gaaaataatg atttagaaga aggagtaaag
     8701 cctcttaaga aaattgctgg tattcaaagt tttgaagata ttaaaaagca cctgtatgaa
     8761 aaagatatag ctatattttt aaattttact agtatatgtt ttggggattt ggcttttatt
     8821 aaagaattgt ccactagagc tataaaattt tcagagaaaa aagttaattc caataaaact
     8881 aaaattaaag acctcgaaaa taaatttaaa aatgatgaag tagaaagtga cgaggatgaa
     8941 ataagcgttg attcaaatgt agggtcggaa agtgacgagg ataatattat agtagatgag
     9001 gatgatatag atctgaatta atagtgccta ttactattga gcggtggtgt acctcttaga
     9061 ggtgtgttgc cgcttttttt gtgcaccctt gtcatcaggc ctatggaaat ggcactatga
     9121 gcgcagtaat cttttagccg tttgaaacga tgacaacgct caatcctgtt cctgaagagc
     9181 caacgattta ttcggtaagt ctgattagta aggccttgcg cgatgttgta gaagggcagt
     9241 tctcgggcat tcgcgtcaaa ggcgaggtca cgggcctcaa acgccataca tccgggcata
     9301 tttactttgc ccttaaagac acagaggctg tgatggatgc tgtgtgttgg cgggggacaa
     9361 agcaagtgga tttgctgcgt gagggccttg agattattgc gtatggcaag atcacaacct
     9421 atcctggtcg ctcaaagtat cagatgattg tgacccattt tgagccaagc ggtgaggggg
     9481 ctttactgca acttttgctg cagcttaagg aaaaattgcg ccaagagggg ctttttgagg
     9541 agcgttataa aaaaaagctg cctgcttttc ccaaacgcat tggtttgatc acatccccca
     9601 caggtgccgt tttgcgggat ataatgcacc gtttgcgcga acgctatccg tgttctgttt
     9661 taatcaaacc tgtgcttgtt caaggcgatg gcgcagcact tcaggtcaca gaagcgattc
     9721 gactcatgaa tgccttggag gatcacctgc gccctgatgt cttaattatc gcaagagggg
     9781 gaggtagtct tgaggatttg tttgcattta atgatgaaaa tcttgtgcgc gcagcttttg
     9841 aaagtaggat tcctctcatt tctgccatcg gtcacgagac tgattttacg cttcttgatt
     9901 ttgtggcgga tgtgcgtgcg cccacgccaa cagcggcagc tgaacttgcc acacccgttc
     9961 ttaccgatat cagattcaaa gttcttgatg catatacacg gctgaatcaa gcggtgcaaa
    10021 atatactcaa acatcaaaca ctcaaacttg agcgctttga gcaaattctt aaagatcctc
    10081 tgcgctatct tttcgataaa cagcaaaaac ttgatgattg gggggagcgt ctacagtttg
    10141 gtattcaaca cacgtttgtg caaaagcgtt tgcaattagg acaagttgac gtaaagccgc
    10201 caagggctat gatggatctt gccacaataa aattaacgca cagcagcgca cagcttagac
    10261 aaaactttga caataaaatc agcggccttt tggaaaaagt tcattacgta gggcgatcgt
    10321 tggagaatat gtcttataaa aaaacccttt cgcggggatt ttgtctggcg catacggtca
    10381 agggtgacat tatcgcgtct ttagcagacg tgcgttacag cacccccttt gttttggagc
    10441 tcgttgacgg cacggtggac gtaaaagcca ttccctcgct taatcaacaa aaaaagctct
    10501 tttaatcttt attcgagtca agccgttcat attctttctg caactcgtcc ttccattcgt
    10561 taattttatt tgtcaggttt ttctgatttt tctcggaaac accgtccatt tttaagtagc
    10621 tttcggctgt ctcaatatga tctttcaaca aacttataat aattttagaa tcagaaatat
    10681 aggtacccac cacagaattt tttataaagt ttttgatctt tgtgtcaagc ggagacattt
    10741 cagccgtttc cttcaaatcc ttcagcgtat ccttccagtt tttaattaga tctttaagag
    10801 tattcgtatt tttgtcagaa atatattttt ctaatctcaa aattttttcg gctgcctcaa
    10861 tatgcttttt caaccagtct gtaaaaattg tgagattagt gggcttagag gtatcgagca
    10921 agcacaaatc aaaaaattct gtataagaat tattgctcca attttggatc tttttattaa
    10981 gtttttgaat tttttcaata tcccttcttt cacgattaac ttgattcatg gcctgattca
    11041 gttcattcat ctcctcatca ctcgccctca caggagaaat atgaagggct acccccccca
    11101 tagatattaa tgacatcagt atcaatatct ttttcataaa aatattcctc tgttaatgat
    11161 taatcacccg tctttataca tgacagagaa aagtaaagaa agggttaagg gaggggggtg
    11221 aattttcttg tcaatgtttc cttttcatga taggatgtgt cgtcgaagtg gatggggccg
    11281 caaagggtat gcctcaccgc gttaatgtta attttttaag aaaagagatt gaaaatgccc
    11341 aaaatgaaaa caaaaagtgg tgcgaaaaag cgttttcgca ttacgggttc tggcaaggtc
    11401 aaaatgggcc aagctggcaa acgtcatgga atgattaagc gttctaataa atttattcgc
    11461 aatgcgcgtg gcatgaccat catgcacgaa agtgatgcga aaaaagttct tacatatttc
    11521 ttgcacggtt aggaggatta agctatggca cgtgttaaac gcggtgtgac ggctcatgcg
    11581 cgtcacaaaa aagtcctcaa agccgccaaa ggttttcgtg gacgctcaag tacttgtttt
    11641 cgcccagcaa ttcaacgcct agaaaaggcg ctgcaatatg cgtatcgcga tcgtcgcgtg
    11701 aaaaagcgcg agtttcgtgg cctttggatt cagcgcatta atgccgctgt gcgcgagatg
    11761 gatatgactt attctcgcct cattaatggc cttaacaagg cggcgatctc cattgatcgc
    11821 aaagtaatgg cggatcttgc aatgaatcaa cccgaggctt ttaaacagct cgttgaacaa
    11881 gcaaaggcag ctctcgctta aagcgattgc attaagtcta ctaaaaaaac tcagccaggc
    11941 gtatggttgg gtttttttgt ttcaaactat aaaaatctct aagctaaaaa aataagtatt
    12001 ttttatctta ttttatgtta aaatccggtc tcgtacaaaa aatagaaaaa ataactatga
    12061 tgccttcgcg ctccttccaa attcagccag aacaccaaac tattgatcgc attgcagcag
    12121 ctattctcct ttttgtggga agcttaacgg ttcttgcagg cgccattatt gcaccgtcac
    12181 ttcctgcaat tgcgcaacac cttgccaccc aaggactcac gattgaaagt gctaattttt
    12241 ggggcaagat gcttgtctcc atccctgctc ttgtgatttt agtgtgctcc ccccttatgg
    12301 gatacgtgat tgatcgtcat ggacggcgta atgttctttt atcagggctt tttctttttg
    12361 ccctttccgg cgtatggggc ggcattcacc cccagttcta tagcctgatt attagccgca
    12421 tgggccttgg gctttctgtc gctatgagta tgacaaccgc tatgacattg atttctgact
    12481 attttaaagg tcacgagcgc gaacgtttta tgggctattt cagcagtgcc atggcccttg
    12541 gtgggattgt ttactttatc cttggaggat tcgttgccac agtaaattgg cgcgcacctt
    12601 tttttattta cctccttgcg ctaccagctt ttgtgcttgc gtattttcat attcctgagc
    12661 ctgttttaga aaaatcacag aactattctt ttggagaaaa gacgaatgcc ttttctgccc
    12721 cccaaaaaac gccaacactt gtgattgcga tgctgtatgc tacagccctt ttgaatatgg
    12781 tgatctactt tgcgattcca acacagctgt cctttctttt gcgtaatatt ggcttcaata
    12841 gcccccttca tgtgggcctt aca
//
LOCUS       DAIAHG010000013        12842 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_13_length_12842_cov_3.475206, whole genome shotgun sequence.
ACCESSION   DAIAHG010000013 DAIAHG010000000
VERSION     DAIAHG010000013.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 12842)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12842)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12842
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13_length_12842_cov_3.475206"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    2563..2572
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3716 a         2700 c         2692 g         3724 t
ORIGIN      
        1 atcaagaggg ggactttgga ggggggcgga ttgctgtgat gcgcaagggg cgtgtttttg
       61 aaaaagcagg cgttgccttt gccgatgtta aagggaattt ccccaaagaa tttgctaagc
      121 aaatcccagg atgcacagac agtacagaat tcaccgccac gggtatttcc cttgtgtttc
      181 accctcgcaa cccttacgtt ccgattgtgc atatgaacac acgttttatt acaacgaaag
      241 ggagatcttg gtttggcgga ggcgctgacc taacgcctgt gatgcccttc gaggaagata
      301 cagcgttttt tcatcacgct atgaaagaag tgtgcgacac ttatgataaa aaggcttaca
      361 cagaatttaa agaatggtgc gatgagtatt tttatattaa acatcgcggt gaaacacggg
      421 gtgtaggggg gatcttttat gattaccttt ttgtagaggg ggataatttt aatgatcttt
      481 ttgcctttac aaaggatgtt gggcttcaat ttctaagggc gtatgccgcg attgcgcgcc
      541 gccgttatca tcatgcctat ggcgatgaag aacagcacgc ccagtttatc aagcgttctc
      601 gctatgcaga atttaatctt gtctatgacc gcgggattaa gtttggcctt aacacaaatg
      661 gcaatattga tgcgatgttt atgtcgcttc cgcctctgtg tgggtgggag taaaaagttg
      721 atttatatcg agtaataatt gtataagata atattataaa aaataacaat ataaaattca
      781 tgtttttatt tattttaata tttctcagcc ttactgaagc aacctttggt aaagaaattt
      841 ctcaagacgc tattttgccc tacaacggga aaaaagaaag agagttcgct ctgtcttatg
      901 gtgttaaaaa acttcctgtt cttttggagt atacaaccga aaaatgcacc attcattata
      961 ttggcgcaaa gcattctttc aatatcaagt cagaaacaca tcaggttatt aaaaaagaaa
     1021 tcgaagattt caatcctgag tttatgattt tagaaggatt tgagaatcgc gggaatctga
     1081 aattttggga aaatgtagtt aagcaaacag acaatgtatc ccgctatgct gcgtttggtg
     1141 aaccaatata tgcaatgtat ttagcaacac gaaaaaacac tcccttttgc tcaggcgaac
     1201 catttaataa agatattttc aagcacctta aggataaggg tttttccaaa gacgatatcg
     1261 ctctttatta tgttctaagg caaataccgc aagcgtatga acgaaaggaa gttactcctg
     1321 aaaacttgtc ttttttcatc aatggtttcc ttcaatctat agacgagcta aagggatatg
     1381 attttaaaac gtgtaatatt ctttttaaaa aactgactaa gaaaagtttg tctcttgagg
     1441 ccgttactga gaggatcctt cgcgaacttt tccctgaact ttttcaggag atcaacacct
     1501 ttcgtgatgt ttctatttta aaaactcttg cagagaaatc taaaaaatat aaaaaaatct
     1561 tgattatcta tggtgcgtca catatctata cacagaacga ggttcttgag gaactttttg
     1621 gaaaaccaaa aataatagac atggatcttt tactcggcga taataataag atttaaaggt
     1681 acgtgagaaa taatgtctga tttgcaccat gctgtttctc ttttaaagga aggcaagctt
     1741 atcgggctgc ccacagaaac ggtgtatggg cttgccgcag atgcccttaa ctccaaagct
     1801 atggcaagta tttttcacct caaagggcgg ccgacgttta accccctcat tattcacacc
     1861 cacagccttg aggcgctcaa agaagatatt attattaccc ccacggcccg ccttcttgcg
     1921 gagcactttt ggccaggccc tctcaccctt gtcctaagac gcagtccaca ttgtcgtatt
     1981 gatcctttgg cgagcgcggg ccttccaaca cttgctgtgc gtattccacg ccaccccctt
     2041 gctttagagc ttttaaaact aataaatcgc cctcttgcgg cgcccagtgc gaatctttcc
     2101 aatcatctca gccctacgac aaaagatatg gtgcttaaag attttaaaga tctttatgtt
     2161 ttggagggag gacctgctgt cgttggggtt gagtccacga ttattggatt agagggagac
     2221 gttccgacac tctatcgccc tggcgggatt gctttggagg cgatcgaggc tgtgattggc
     2281 acaattgcac aaccaggaaa aggcattcat gcaccaggca tgcaaaaaaa acattatgcg
     2341 ccaaatcact cattgcgcct taatgcggta tcgaaaaatg agggagagct tctccttggc
     2401 tttggcgata caaaaaacgc agaccttaat ctcagcccta ctggtgacct tacggaggct
     2461 gcgtcgaatc ttttttatat gctgcacctt ttggatgcgc gtccctcaaa ggggattgct
     2521 gtctccccca ttcccatgat agggctgggt cttgcgatga atnnnnnnnn nnaaaaaaga
     2581 tcccaaaaac tatttttttc taagcctgca ttgttattta tgctatgtgt ttctgtgtgt
     2641 agataattaa aatcataagg aatatttaaa aatgttttca aaaacactca tgagcttttc
     2701 ccttgcaatc actcttggcg caggtgcagg cgtggcatcc acccataaca atgacaatat
     2761 aataaatagt gatgaaaaag atcacctcac gctgaatgct cagaacatag ataaatctga
     2821 ggaagagaaa agctccgacc ttaagaaaaa agaagaagaa atggcgcagc aattaagaga
     2881 tcagttggaa aagattaaaa gcgatttggg tcttgatgcc ctctatgcat tttatgatag
     2941 cctcgatgat gaagagcaga aggagattaa taaatattgc agtgaccttt tcaacgatta
     3001 attatctcaa aagggggccc ggcctccttt cttcacagat taaaagtcta gaactaaaag
     3061 tccagcacgc caagacttct aagcgacaca gcatcgctgt gccccaccac aatatgatcc
     3121 agcaccgcga tattcaaagg cgttgcgcct tttattacct cgtgtgtcat aagaatatct
     3181 tcctttgagg gcgtcggatc gccgcttggg tgattgtgcg caagaataat ggacgacgca
     3241 cccacctcaa gggagcgctt gagaatttcc cgtggataaa tcgcagtggc atcgacggtt
     3301 ccttgttgca taatttcatc gacaataatg ccattgccgt tgtcgagaaa aagaactaaa
     3361 aggctctcaa tagggctaaa ggcaatacgc tgacggcaat acgccatgac gtcctttgcg
     3421 cctcgaatgg ttattttttt ttgaatatct tgctgcagca tacgtttgct taacacttcg
     3481 acaagcttga gtgcgaaaag gacattgtct cccacacctt ttatttctaa aaggcgcgag
     3541 gaaggcgcat tcaaaagctc agaaaatgtt ggaaaatgcg caataagttt tttagcaagt
     3601 ggcttgacat ccgcgcgcgg tacggccaaa aaaagcaaaa gctccacaag ttcgtaatcc
     3661 aaaagccctt caggcgcctt taaaaatttt tcttttaaac gcgccctgtg gccaaggtaa
     3721 tgggaatctt cacgcataga atttaaaaac tattttttct gaggattctt ttgcggcttt
     3781 tctggtgttt taggtttttc gcctgctttt gacgcttgag gttgtgcggg ttctgttttt
     3841 ttaggtgcag ctttctcctc ttcgggcttt ttttcctcaa gtgtataggg cggctggctc
     3901 gatgattcct cctctggcat cggtggatta gacggaggat tttgaggtgt ttgggccatg
     3961 gttcctgggg tttcaggaac ttttttagca ttttccgctg ctgtctctgc caatgactta
     4021 aaagagagtg ccacgtattg tggaggaaga ccacgacggg tgaccgttaa aagaacgctt
     4081 ggccgtttag gatcagtctc atgaatttct ttaatggctg ctccgatttc ctcaggtttt
     4141 gtcacgggtt ttaaattaat ttcattgata acatcacctt ttttcagccc cgaatcctca
     4201 gcacttgaat tttgcttaac atccacaacc aaaacaccgt ttttcaaagg aggaagcccc
     4261 ggagcactcc ccggattttg ttgtccattg acaatggatt tcggcatagg cacaaggccc
     4321 atgccaagga cctctgtaac tttttcagga ggcgctttaa tggtttggcg agccgcatca
     4381 tccattgtta aattggaggg atattctgcg acgacgacat taaaggtcat ttccttaaag
     4441 gacatttcat tattattatc cctcaaaacc ttcattgtga ccgtttttcc aacaggggtt
     4501 tcactcacaa cacgggagag acgtacttta tcgctgagtt ttttcccatc aaactctata
     4561 ataatatcat tttctttcag accaattttc tctgcaggac tccccttatt aatagctttg
     4621 acgatcccgc cccgcgtctc tccaagacca agactctcag cgacctcagg agaaacaggc
     4681 aatacggaaa cgccaagcca gccacgctgt gtatgaccaa actgaataag ttggtcaatt
     4741 gtagacttcg ccagattggc aggaatcgca aaaccaatac caatgctgcc gccactgggc
     4801 gagaaaatcg ccgtattaat accaatgact ttgccctctg tattcaggag gacgccgccg
     4861 gaattgccca tattaatcga cgcatcgtgc tgaataaagt catccacaag gtctgttgat
     4921 ttattatttt tggcacgcaa aacaatgtca cgccccttgc cggaaataat ccccgaagtc
     4981 accgtacttc caaggccaaa gggattgcca attgcaacaa taaactgccc gacctttgcc
     5041 tcatcggaat ttccccaact gagcgttgga agtttgcgct tttcagaggg caagctatca
     5101 agtttgacac gcaatacagc aagatctgtg cggtcatcat tcgcatgaag cacagcatca
     5161 atttctgttc tatccgcaag aatgatcgta atcttagatg catcggcgac gacatgatta
     5221 ttggtgacga taaacgcttc tttatccgac acgcgcacaa taaagcctga tccgacggat
     5281 tgaacacgct tagggcggtc ttgaggttca aaaaagtcct taaaaagctc ctccagcggc
     5341 ggaaaaccgg gacccatttt aggcatttcc tgtgctgcta gcgctttttg atccacgact
     5401 tgtgttgttg agacattcac aacggcagga atcgctaatg ctgcaacttc agaaaatccc
     5461 ctgcgaaaat cagagatagc cgttttttca gaagaagctg cagcagagct ttcaggaata
     5521 ttctcagcat gacctgcttg aggacaagga acaatttttt gctctattaa gcgcacttgt
     5581 tctgctagct gttgttttac ctcaagattc acccgctcat ttattttttg atccaaggcc
     5641 aactgcattt tttgctctag aacaccttga agttgcttga gagcatttcc tgaaatcgtt
     5701 aaatgggtgg gatttacagc tccctgttct tgtccccctc cttgtacacg ttgcttaagg
     5761 gcatccatcc gttcacaggc aggaagtgtg aaaagtgtaa gaaccagaat aattgttgca
     5821 aaacggtgaa tcatgattgt ttcaaagcct tttattaaac attaatcttt catctgctta
     5881 gtaggatgac cgaaaaatct aaacaaatcg ttatcagttg ataaaattaa agatgtttct
     5941 gtggtcaatg cttcatcata aatttgcata gaacgataaa aagaatagaa ctgcacatct
     6001 tttccaagcg tacttgccac acgattaatc gcctttgctt cggcctcacc acgaatgagt
     6061 tgcgcatttt tctgtgcttc tgccaaaata atagtttgtt cagcatccgc ttttgcccga
     6121 ataccttgcg caatttctga tccttttgca cggttttcag aagcaatacg attgagctct
     6181 gcattcatac gcgcaaaaac agcattgcgg ttttctgcgg gaagttctgt ccttataata
     6241 cgcacatcaa taatttcaac accaagacct tttgcaagag ctttgacctc ttcttcgatg
     6301 gttcgcataa tcgccgaacg cttttcagag agaagttctc gcaaaggtat tttaccaagg
     6361 acattacgga cagagctact aataatggtc tcaagacgca tacgcacacc atcttctgtc
     6421 gcaggtttga tggtttggaa aaaaagcaca ggatctttga tgatataccg ggtgtaggtg
     6481 tcgacgataa ggcgcttttg atccaccgtt gtaataggaa tggggggtaa atcataatcc
     6541 agcaccagct tctcatacat aatcacatca tcgatgaaag gtacgcgaaa cttaaggcct
     6601 ggctgagtgt aaacatcaac aagtgcacca aagcgcagga caatcgcctg ttttgtttga
     6661 tcgactgtaa acatcataaa attcaaaagg acaataatgc cccccaaaat aacaaggaaa
     6721 ggaatcgtta gacgatgagt cattattttg tctccgtttt agtggaatgc gcctcaggcg
     6781 caggtttttt gagttcatta atgggcaaat agggcaccat atttccaatt ttaggatcga
     6841 gaataatttt aggcgcagac tttagaaccc gttgcattgt ctccatatag gaccgcatga
     6901 gcgccacatc tttgtttgtg ctataggctg tgtagaccaa gttaaaccgt gatgcttcac
     6961 cttccgcttc agccactgtt ttttgtgcat aggcctgagc ttcttgcttg atgcgctcgg
     7021 cattaccgcg ggccacagga ataatctgat tactatagga ctctgcttca ttttttaaac
     7081 gatccgcatc gaccaaggat gcttggacat cgttaaagga ttcgataact tgcggtggag
     7141 cctcgacgcg ttgtaactgg aagctcacaa tttggacacc gagtttatag gagtcgagta
     7201 ttttttgcag taattcatgg gcacggttac taataccctc acgcccttcg gttagcgcag
     7261 agcgtgccgt tgtttgtcca aatacttcgc gaaccgcact ttctgcagcc acagtaatga
     7321 gcccctcggg atcacgcgca gtgaagagat attcactcac atccttaata cgccattgga
     7381 cagaatagct aatgatgacc atattttcgt cggatgtcag tgtcagcgcc tgctcggaat
     7441 cgccactttt ggcttctgca gtcatccccc catcgatgcg gttaatcgct gagacttttt
     7501 gcacacgtac agattcaaag ggtgcaggta aatgatattg caagccaggc cctacgatac
     7561 gcaccatttt accaaagcgc aagacaacac cgacttcgcc ttcttgaaca cgaaaaaggc
     7621 caagcgcaaa ccatacaacc aaagctccaa ggccgataag tgctaagaat ccggcacctt
     7681 ggtgaaaagg agagccacct gatccccccg atccttgacc gccgccaccg ccgcccatca
     7741 tttttttgag gcgctcttgg agtttgtcaa tcatctcatc tgctgcgttg ccactatttt
     7801 tattccatgc attgccgggg ccatcgccgc ttttacgggg cccccaaggg tttttaggat
     7861 cgctcattat atcttaatat cttgatagaa gatgttatat agacactatc ataactttat
     7921 cgcacgcaat cggaaagaga tataaaaaat atttaagaaa aatcattgtt ttatgagcaa
     7981 tctgcttact cacctttagt tataaaagaa gaacagatca aagtttgtaa gcattctgtt
     8041 gaaatctgtt ttgggaagtg cgttattacg cttgagaata ctttttaata tttcttcatg
     8101 gaaagaaata cccataatat tttttatgta ataattatat ttattgagat agtttaaaaa
     8161 ctcttcttca ctagcattgc tttcatcaat gaaaattttt cttattttat tgtttttcat
     8221 gaactctatg aggaaaggaa cgccctcatt gcccatataa cttattccaa aatcaaaagt
     8281 attaaaatct ttccctttga cacgctcact taaaattttt atcccatcag gtccccacat
     8341 attattctga agatataact catggattgt attattattg atgccatccg cgataatctc
     8401 aatcacctca ggcggtgcag gctgtaggta aaatgttgat aggtgcatag agtcaacatc
     8461 ttggccagtg aacttactga aaaataaact aaacgcctta ctcacatttt ccttcatata
     8521 agggccattg acttggatct gaactatttt tttcccagtt ttaggagata atttatagtt
     8581 aaccctcagc accttttcca tatttttgcg ctctttttta gaaaaaaatt tctcaaggag
     8641 gtcagcccgg tcttttgcac tatcgtctat cttttcctct actttctcct ttggcttttt
     8701 cgtcagtttt ttaaagggtt ccaagaaaat cgtcttaggt gtttttttct tacagttagg
     8761 ataatcctgc tgcgcccaca cagagaagtg attggacaaa caagtgaaca tcaaaaaaga
     8821 aacagtaagg ctaagtgttt taaaaaaatc cataaaaaaa tactccttat aatcacgctc
     8881 ttttatcgct atagttttga ctaagaagat taataaaaaa ttaaaaaatg caaacccaaa
     8941 atcacacttt tcgtgtcaaa aaatatgtcc ctggtgttaa aaaaataatc gcaatcgcct
     9001 ctggcaaggg aggggttgga aaatctaccg tcgcgattaa tctctctgtg atgctggctc
     9061 agctggggta taaggtggga ttgctcgatg cagatattta tggtccctct gtgccgatga
     9121 tggtgggatt gcaagaaaaa cctactgtca atgatgctaa aaaacttatt cctcttgagg
     9181 cacatggcct taagattatg tcgattggat ttcttgtgaa ggatgatgcg ccgatgattt
     9241 ggcgtgggcc tatggtgcaa agtgctttta tgcaaatgct ttttgatgtg gcgtggggag
     9301 atcttgattt tcttatgctg gatatgccgc ccgggacagg cgacattcaa ttaacacttg
     9361 cgcaacaagt tcatgtggat ggcgctgtga tcgtatcaac acctcaagac atcgccttaa
     9421 ttgatgcccg caaggggatt gctatgtttc aaaaggtcga ggtccccgtt cttgggatta
     9481 ttgaaaatat gagcgcgttc gagtgccccc attgtcatca tacaacagct atttttaatg
     9541 aaaaaggtgc tgaaaaacag gccaaagatt taggcattcc ttttcttggg gcaattccgc
     9601 ttaagctttc gattcgcgag ggaagtgatc gtggaaaacc tgccgtctta gcacataaag
     9661 atatttttga gctttataaa aaaataagca tgcatttttt aggataggaa tgatgagggg
     9721 gtggcagcgc tgcaaaatag aggagattaa aaagggggat aaatgcttaa tttttcaaag
     9781 gaaaaaccct aacaattcaa gcccggctaa agaattgccc taaaaattcc acatgttgca
     9841 cgacgaattg ctttttatga aaagttaaaa taaaattgca cacaatgttg ctttggtttt
     9901 tctgctctgc attcatgcgc acttttatta ttggtacccg aggaagccct cttgctcttg
     9961 cacaaacgcg gtttgtcgaa aactatttag cctctcaaaa cattccttat gtgattcaca
    10021 caatcaaaac aaccggggat attattcaag ataggcctct ttatgagatt gggggaaagg
    10081 ctctttttag caaggaaatt gagcggtgtt tggaggaaaa aacaatcgat attgccgtgc
    10141 attctctgaa ggatcttgaa acgccgcgcc ccaaaggatt cgaaattgta gcttatctct
    10201 ctcgtgcgga cgcccgtgat gtgcttgtga cgcattttaa ttatgatgag aataaagtgt
    10261 atggatgtca gattaatgat cttgcgcaga aaagttttgt tgggacgtct gcaccaaggc
    10321 gtatggcact cttaaactat cacaggccag atcttcagat tatccctttg cggggaaatg
    10381 tagggacgcg tcttggcaaa ctcaatggaa acaaggaaaa ttacgatgcg atgatttttg
    10441 caaaagcagg gttggatcgc cttgggatcg cgattgaagg taccgctatt ctctcgaaag
    10501 acacgatgat tccagcagca ggccaaggca ttgtggcgtt agaatgtcgc tcagatgatg
    10561 aagaaacaaa aatactttta agggaaatga atgatcccac agcgcaacta atggcgcaga
    10621 tcgaacgggg gtatattgag cgcttagaga atgcttcctg tcgctcagcc gttggtattc
    10681 atgtagaagt taattatcaa aatctacacg ttcgcacaat ggttgcaaac cctgagccca
    10741 aaatttttaa ccatcatttt gagcgagaaa cctcctttga ggcgatttgg cagacactta
    10801 ttgcatgcac agggagcagg ctaaacactt tccataagga taatccatga tcatcaaatg
    10861 cttttttgtt tttgtttttt ccatatcttt agcgtatgcg ctgacaaaag aaacggctgt
    10921 agaacaatgc agtgcacata ttgattgtct caaaaaagcg cctctgtcgt cccttgacgg
    10981 ggaaacgcaa actttcttta aaagaatgat tgtccaccta gaaaccattc ttttgaatct
    11041 taaaaaaaca gaggatatta aagaaatcta tctttcaaga cagagctgtg ataaaggttt
    11101 caaagccgca cagtggattg tggatcattt ttcaggggct caacctgccg ctcaaaacca
    11161 agaaaaaaag aatgacaaag acaagccgaa tgcagaaaaa ataacgccgc cctcaaccct
    11221 aaaaaaggat acgcctcgtg atgaatctcc ctcaaaaaaa gcaaaagtca ctccccctgc
    11281 aaagcctgcg tgaaattatc gatcactatg atttttttat ttttgatctg tggggtgttg
    11341 ttcacaatgg aaagacatcc tttccagggg ctattgagat cttaacgcac ctaaaaaccc
    11401 ttgggaaacc tgtttacttt ttgtcaaatg caccccgccg cacgaatgct gcaaaagcac
    11461 agcttgttga tcgtggggtg cccctggatc tctatacaaa tctttttaca tcgggtgaag
    11521 atacgtttga gcacttgcgt gaccggcctg atgcttttta caaaagcctt ggttctaagc
    11581 tctatcacat gggccctgac aaagataaaa gcctttatga cacacttgag aattatacaa
    11641 atacacctct ggaggatgcg gattttattc ttaatacagg tactctttcc tttgaggata
    11701 cccttgagat gtatgtgcca gaacttgagg aaggtagggc acggaagctg cccatgattt
    11761 gtgcaaaccc tgatctgatt gttctttttg gagaaagtga agcgctttgt gccgggcgaa
    11821 tagcaaagga atatgaaaaa atgggaggaa cagtacgcta tcatggcaag ccgtttgtga
    11881 gtatttatca aaaaatgttt gagcgtatgc acacgaatga ccttggtaaa attcttatga
    11941 ttggcgactc gctgcgcacg gatattaagg gtgcaaataa tgctgggatt gactcgattt
    12001 ttattacaag tggcattcat caactatctt gcgtggatga tgctctctcg ctttatgata
    12061 catacgatgc gtatccaaca tacgtgatgg aaaaagtaat ttagcttgtg tagacaataa
    12121 aaatccttga tcttattcac tagagcattc aatgcagatt gaatagagga tgtgtgatgt
    12181 acaaaacgac aatgcctata attttctcgg tggggttttt gactttttct caattcttaa
    12241 cgccacctct tttggcgtct gaggagaata aaaaagaaga gggaaatgaa aaagaaaatt
    12301 ctaaaaacct aaaatccact catgaaaaag atgaaaagaa aaatgaaaag aaaaatgaag
    12361 aaacatctag gattctgaaa tacgcttttg gcatgggatc aacgccaccc gagaaagatt
    12421 ctttttggaa taacaaagat tttaaaactt ataaaaaaaa ttacgattac ctagatgagt
    12481 atgaaaaaga taaggtgggt aatgagttaa atcgacgcac ttactttaat caaaatactt
    12541 ttatgagtcc atcgaccgct cgtttcactg actactctta taatcttgcc actgctggca
    12601 ctggtgctga tcataaaaaa aagaaagttg ttaagaagaa aaagaaaaat agctctgatt
    12661 tcgattctga ttctgactaa tgagaggggg agctcccctc tctcaaataa ttaagtataa
    12721 taaaacctaa aattgaaccg ccgcttaaaa tccttaaact ttccctggct ttggtgcatc
    12781 tccatctttt gagtcttttc aattttctgg accatctcct tgttcttttt atttttatcc
    12841 tc
//
LOCUS       DAIAHG010000014        12429 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_14_length_12429_cov_3.017730, whole genome shotgun sequence.
ACCESSION   DAIAHG010000014 DAIAHG010000000
VERSION     DAIAHG010000014.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 12429)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12429)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12429
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14_length_12429_cov_3.017730"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3734 a         2406 c         2772 g         3517 t
ORIGIN      
        1 cccacaatat cacctttttt caaaggggcc tggagcggtg aatcaaagtg aattttaata
       61 tcaactttgc tttgttctgt gcgcggtaaa agcacactca cgtcatccgt taccacaaga
      121 ggaactgttt ctttgacacc aagccataca ggtgcggctt tttcaacaat atctcctgcc
      181 ttatagagtt gtttgttgat aaaattttgc tttgcccaag cgagaatcat ttttgcatca
      241 ttcgcccgtg cttgcattgt tttaaggcca ttaatcacca tgatatagcg ttgccccata
      301 tctttaaagg atgccacgat accatggcca ccatcatccg tgtgtcccgt tttaatacca
      361 tcacagccca tatcactgta aagcagagga ttgcgattgc cttgtttgat ggggcgtcct
      421 ttactatctg tcccaaaagt aaattctttt tcaccataaa ttttatagga ttcagggtga
      481 tcgttgataa tacgaagacc gagagccgca agatcccgtg ccgtactgta atgaccttct
      541 tgcggccatc cactggtatt cataaattgt gtgtttgtca ggttcatagc ttttgcagcg
      601 gcattcatct ctgtaacaaa ttgttcctca ctgccggcaa tcccttcagc tgcgacgata
      661 caggcatcat tgcctgattg aataataatc cctcgtaaaa gatcgtcaag ccttaccatc
      721 ccaccaaggg gaacgaacat tttagatccc cccatgcgcc atgccttttc actcacaggg
      781 aagctcatat caagagagag ctcacctttt tttatctttt cttcgatcag ataagaggtc
      841 atcatttttg acatggacga aggcgtcatg cgttcatccg catgcttttc aagtaacaca
      901 cgtcctgtgc gtgcatcgat caaaatcgct tgctttgcca gcagtttaat ggctgtagct
      961 cccggtgcaa cagtcacatc atcggcttca ctctctgttt ttttttcttc tgttttagag
     1021 gaatcttctt gacctgtgct cctcgttttt tttgctttgg tcactttttt tgtgggtgag
     1081 gggttggccc aaagcgtctg cgtctcaacg acaactaaac tgaagaagga gaaaagaaaa
     1141 aaatagtgca tgactcaaga cattttatga tggaaatttc tgccattcta acgccatcaa
     1201 aggaaagagc aacctttttt ctgtattcat cagttatttg tccatagagc tctctaatgt
     1261 ctggaagaaa tgtttaagcg cttgtaagta cagctctggc tcttccacat ggggaaggtg
     1321 agcgcttttt tcaaagataa cgagctgact cttttgaagt ttttcaccta tacgctctaa
     1381 tgtttctggt gtggcctcat caaagcgccc acaagtcaaa agaataggga tatttaagtg
     1441 acaaagatga tcaatcccat caaaatcttt taaatgtcca gtcaccgtga attcactggg
     1501 cccccacatt gttttgtaga tatcaaaatt aatcgcattc agcgcatctt ttacatcctg
     1561 tggcagcggg tttaaacgac aaaggaacgt tgcattaaag gctttggagg cctcttgata
     1621 gtcgtgtgaa tcggttgtgc cttgctgttc gtgataaaga attttttctt gaacgtcttg
     1681 gggaagttgc ttaaggagac gctttgtatc ttggatccac agttttgtgt tgaggagagg
     1741 gctcgctaaa acaaggccta taattttatc agagtttttt gaggcatact ctgttgcaag
     1801 ggcaccgcca aaagagtgtc caaaaagaat aaaatgttta aggccaagat gttcgactaa
     1861 agcctcaaga tctctcacat aatcggataa ttgccatgaa tgagcgtttt tttcagtgac
     1921 gtgcgagcga ccacagccaa gctgatcata aaaaataaca ggtcgatcgg taccaagtgc
     1981 tgagagggat ttaagataat tatgcggaat ccctggccct ccgtgaagga taaggagagg
     2041 cggtttattt tctgtttttt tggtggaatg gatttgaaac caaagctctc ctccttgaac
     2101 agggataggt ccttcctgta cgtgaagaga cgcatcttta tcagaaaaat agttcaatcc
     2161 tacggccata aaaatcacac caatcaacaa taaaaatttt cgaacccaac ggcgttgcat
     2221 aaatcatcaa gaattatcag gggacgatgg agcattctcc aacataacat aggcctgtgc
     2281 gctctcaaat cctttttgga tcagttgctc gccatcgacg gggtgggcct tgataaaatc
     2341 aatggttttt tgaagatttt ctaaatcaag attttctggt tcatcctcaa aaagctccac
     2401 gagaccgatc catattttat aattgagcga tgtatcaagc tcgggataat gatggcaaat
     2461 atcttgccaa acacctcttg ggtttttttt aaccaaagcc agtgcttctt tcattgcact
     2521 aaggaatttg tggatatagg ctttgccttc atcatctttg tgcgcgaaaa aaataaattg
     2581 ttcaaaggga ggaatgccaa attcttcgta ggcataaatg cgtatgggct tgttaaattg
     2641 ttctgcaatt ttcgcatcat agggaagcgt tgctgtaaac atcgcatcca cttcaccgtt
     2701 cataaacgca tgagctagcc ctttttgaaa agcgataaga tcaatgtcac ggcgcgtaag
     2761 cccctgtgtg gttaaaattt tatctaaaac aatcagagaa aacgctggat tgcttgtgga
     2821 gaatcctatt tttttatttt tgagctcttt taagtgagga agcgactcat tcacaattaa
     2881 aacctccaga ggttttccaa gaagggtccc tatttttttc aaaggcaggc ctttattcac
     2941 acggacaatg tgatttgcac tttttgtaat gccaatatgt gcttgctttg aggcgacttg
     3001 gcgactactt tcctcgccac cacttgcggg agaaagattg acatcgaggc cgtatttttt
     3061 aaaaatgcct agtttcagtg ccaaaagaac cggtatttga catggatcaa ggtgatagcc
     3121 aagggtgatc attatttttt caagaggcgg ctttgcaggg ttttgagcag ttgcaaaact
     3181 aggataggag gaaaaaaagt tgagaataaa tgttaaaaga aaaatgattt taaacatctt
     3241 tttaatgtcc tttgcttttt taaagatagt gaaggttaaa tttatgataa atatagcgat
     3301 ggagagagtt agatagaaaa tagaaaaaga gactcatcgc cattaaaagg aaaaggcaac
     3361 caaacatcaa cgctaaatcc atttgcccat agcacaacat catgacatag ccgagacctg
     3421 aggatgatcc aatccagtca caggcaatca cactcaaagg agcgtgcatg agcgcaaggc
     3481 gaaatcctgt aaaaaagggg ggcaacgcat gggggaggcg tatgtagaaa aaagttggta
     3541 accaagacgt tgcacctatg ttgctcaaga aatctttatg ataatgggga agatgcttga
     3601 gcccctccaa aagtcctact gtcatgggaa aataacaact gagactaatc agaaaagctt
     3661 taggccctat gccatgacca aaccacaaaa gtaaaagggg ggctgtcaca aaaataggaa
     3721 tatttttcaa agtgataaga tagggcataa gaaaatgttc aagccgttga aagcggtcga
     3781 gaaagagact tgttgtgagt gcgagaataa agcctatgga aaggcctagg ctaatttcaa
     3841 gaaagctaat gctcaagtga aacaaaagat atgatcgctt ttctattatt ttctgcagga
     3901 tcatcaaggg tgagggcagc atataatcgg gaatctgaaa aagaaaaatg gagccctgcc
     3961 acactatgaa aaagaaaaaa agaaaaagga gtctatgcgg cataatacgg ctcttttatt
     4021 gctagaggtg cgagcatcaa gggcttattt ttgagggtat agcacgcgtc agcaagggca
     4081 tgcacttcat cttcttggtg cgtgacaaaa agaacggttt gtccttttag tatttttttt
     4141 atatgcgaaa acataccttt ttttaacgct tcatccatat ttgtaaaagg ttcatccaaa
     4201 aacagcaaag agcgattttg atacaaggct cttgcgagga ggatacgttt tgtcatgcct
     4261 ttagagagtt cgtgcgggta aagatttttt gtatgtgtta actgaacaac ctcgaggaga
     4321 ccttgaatac gctttgtatc caacggattg tattttaaaa ggtcggcgag acctatattt
     4381 ttttccacag acagccacgg gaggaggcga tcatctgcgc cgaggaaagc aattttattt
     4441 tttaacggtg caaacaatga atgttttagg caattgctcc cttcatcttt tttattggca
     4501 tggagtagtc cagaaagata gcgcaaaagt gttgttttcc ccatgccact tggtcccata
     4561 agagcagtcc acttattttt ttgaaggcaa aatcgttgct cagaaaaaca caaagggtct
     4621 aggctatttt ttgagtaaaa agagcaataa ggaatatgaa aaatataatc ttttgacata
     4681 aaaaatccgc tttcctacgc tggtatcatc cagatcaggt tcgaagggtt gagtagtttc
     4741 actctctcag ctttttacgg cacccctagg gttgtatctc aagggataaa ggatttttat
     4801 tgaaaagaca agcagaataa gattcttaaa aatgctcgtt ttttatcttt ttagacgctt
     4861 tgacaatgtg tttttttaag aggaggagca cccgtttgat ttttggattc tctccttttt
     4921 tcttggcata ggctttataa gaatgaaaaa ggatattctc tatatcgctt ttgcgaaagg
     4981 tgttttcttc aagcgtctca agaaaagaaa cgttcttttc aagggcttcc atggctctaa
     5041 aatctatttt atttttctta atagcgttcg tgtccgtctt tagtatgatt ctgttcaaaa
     5101 tttttttaga aggtattttg tagcgttgta tgtatgctaa ataggaagga tgtaaaagaa
     5161 ggctctcgtg attgtatgcg atggcacgag aaagttttcc tccccatgag gtatgggaag
     5221 aagcaatgga caaaagaata agggcgtagg ctatttttct agacataggg ggatattcct
     5281 tttaaaaaag tcacaatttt agcatagtgc atgaaaaatt tagggtctag gaccgaaaat
     5341 gaagggcctc ggcaaggtgt tgggggtgaa tatcttttga aacgtccaga tctgcaatgg
     5401 tgcgcgcaac ttttaaaatc ctgtgaaaac cccgtgcaga gagatgcatt ttttcagccg
     5461 ctttgaggaa taagttttca cattcttttg ttaaaggcgc aatcatttca agttgttgtg
     5521 aggagagatg cgcattaagg gtgttgtggc taaaattttc tttatggctc cgtttatatt
     5581 gaagatcacg tgctttttta acacgctctg caatcatagc agacgattcc tcgtgcgcat
     5641 ccttttgaag gagatctgcg ggaagaacat cttctacata gacttttaag tcaaagcgat
     5701 cgagaagggg accagagaga cgttgttggt attgctcacc acataatggg accctcgaac
     5761 attgctttga gggattgccc atatagccac atttacaagg attcatggcg gctacaattt
     5821 gcacctttgc aggatagcgc accatattat tggcgcgcgc aatggagaca tagcccgttt
     5881 ccaaaggttg ccgcagcgct tcgagtgtgg aacgatgaaa ctcaggaagt tcgtctaaaa
     5941 ataaaacacc atgatgggca agggaaattt cgccagggcg acacttagca cctcctccca
     6001 caagggccgg aagcgttgct gagtgatggg ggtcacggta agggggctct gtgatgaggc
     6061 cagactcagg cagaagaccc gcaagactat gaatcattgt aacctcaagg gcttgttctg
     6121 ctgtaagttc gggtaaaatg cctgcaaagc gcagggcaag catggatttt ccagatccag
     6181 gaggccctga aagaagaata ttatggccac ctgctgctgc aatttccatg gctcttttgg
     6241 ctaatttctg ccctttaatc tctgaaaaat taccagagtg ataggaggga tgatttttat
     6301 cattcttttt tttctcaggg gagtattgac gatcaatgtc ttcgaaaagt gtgatttttc
     6361 catgttttag gttgtggata acgtcaagaa gagtgcttcc tgcgatgata ttgtgaaggc
     6421 ccgaccaata ggcttctcgt gcacatgttt tagggcaaat cagacctttt ttaaccgcat
     6481 gcgccgcaat agaacaaggg agaacgcctg ggacgggtaa aatttttcca tcaagaccta
     6541 tttcacccac gacaaaatag tccgctaaag cttcactatc aatcactccc agcgatgaaa
     6601 gaagtccaag agcaatgggc aaatcatagt gagagccttc tttttggaag tctgcggggg
     6661 ctagattcac ggttacacgc tgaggcggca aacttaagtt catgctatga aaagcagcac
     6721 gaatgcgttc ttttgactca ttcacagcct tatcaccaag gcctacaata ttaaaggttg
     6781 gaagacccgg tgcaatttga acttgaacgt cgatcgtaac ggtatcaacg ccttgaaagg
     6841 caacagtgct gcagcgcgaa gtcatggacc tacatctttt taatcgcttt atataatagt
     6901 cattgtgaag agagaaaagt taattttata tgaagaaaaa aaattctatt tttacttttt
     6961 atttactttt tcgtgagatg atttggaaag gattggaatt gaacaaaaag gtttttttac
     7021 atgacacagc tttttcataa actcggtttt gcaggggctc ttggtactag ttttattgga
     7081 ttttgggcgc tttttttctt tgggcatttt acaggatacc aaatgattct tttggcggta
     7141 tttgcttcta tagggcttgt tggatggtat tattcgctct cttacgccaa aaaaacgtcc
     7201 tcgacactct ttaatgcttt tcagcgcggt gactttaatt tcacagacac tgagaaaaac
     7261 tatttttctc aaatacccgg ttttacacag gttattgacg attttaataa tgaaaaacaa
     7321 agtaccacgc gccttaattt tgcaatagag tgtgtgacat cttctgttat ggttgccgat
     7381 aatcaataca acattgtcta ttgtaatcca tcgatgatga cggcttttcg aaaagttgaa
     7441 gcggacattc gaaaagtttt ctcccaattt gatgtgaata aactgattgg cacaaatatg
     7501 gatatttttc ataaaaatcc agcccaccag cgtggtctgg tgtcttcttt aaaagaaacg
     7561 gttaaaacac gtattaaagt gggtccttgt acgtttgatc ttgtggcttc acctattttt
     7621 gataaaaatc aaaaacgcca cggaacggtg attgaatgga aagaaatcac agcagagctc
     7681 aaggtagaag aggaaatttc atcccttgta gctaaagtat caacgggcga cttctcttac
     7741 cgtattgagc tgtcggataa acaagggttt atgcgtacac ttgctgaagg catgaataaa
     7801 tttacggatg ttattaataa ttctctcaca gatattcttg ggttcttatc gggtctttct
     7861 gaagggaatc tatcgacgcg catgaagggt gagtatgccg gaatgcttga ggaaatcaag
     7921 caaaatgcaa attatagtgt ggaaaaactt gaaggaacca tagcgaatat tattgaagga
     7981 acaaagcaaa ttcgcgaagc ttcaggggaa atttctgcag gatccaatga tctttcagaa
     8041 cgtacagaaa cgcaagcttc aagccttgag gaaacagcag catctattga agaaattaca
     8101 gcggctgtta aaagcaactc agaaaatgca cagcgcgcaa caaaaatgtc ctctgatgcg
     8161 tcgggtcatg cttctacagg tgaaagggtc attgaggagg ctgttcaagc catgggccgt
     8221 attcaagaat cctctcaaaa gattggtgag atcaccaatc tcattgatga aattgcattc
     8281 caaacaaatc ttttggcgct taatgcaagt gtggaggcgg cacgtgcagg agactttggt
     8341 aagggatttg ctgttgtggc ccaagaagtg cgtgcccttg ctgagcactc tgctcaagca
     8401 tccaagcaaa ttaaagccct tattgaatcg agctctgaga gtgttcaaga gggatccgat
     8461 cttgtggtac aggcaaaaca ctctcttgcc tctatcgtca cttctattaa agaggtatca
     8521 gagattattc actctattaa tttagcaagc tctgaacagt ctgtgagtct tgatcaaatt
     8581 aaccaaacag tcacacaact cgatgcaatg acgcaaaaga atgcagcact tgtgacagaa
     8641 aatgtggcaa caacagcgtc gatggatgag caagcagaaa gccttcgtga catgcttggt
     8701 ttctttaaaa ttggtcagac aacgacactg ggtcaaggaa gagggagtgg gacaacatct
     8761 caacctacag cctatcacta agtaacgact ttataagaaa tctctttctt tcccctgatt
     8821 ttctttcaaa gagatcaggg aattttttgg gaggaaaacg tgtttttaaa aacgggctaa
     8881 tttatccagc tgataggtaa gctgatgctg cagagggggt aaaatagagt gcttcaaaag
     8941 ggcttcttga agaagcgcat aaaattcttt taaaaattca ccgcggggat cgggttttgt
     9001 ccgaatggat gcacttatat gaattgctgt atctgtaaac tttgaaatac ctgaaatttt
     9061 aaggggctcg acaataattt ttccaaattt tggatgttct ttgatcgcct gaaaagcttc
     9121 ttgcaaaacc tgatcaattg cagcaagaga agttgtgtgc gacacagcaa gatcaatact
     9181 ttgctgtgta tagtgtctgg agcgattaat gattgatgtc acttcagaaa aaggaataga
     9241 ttgcacactt ccgttatcgt ggcgtaagaa aacgctgcgc agactaatcg cctcaacaag
     9301 gccagaataa ttgccaattg caacgtattc tccgacttta atattcccct caaaaagggt
     9361 gagaaagccc gtgatcacat ctttgaccat cgtttgcgca ccaaaggaaa tcgccaaact
     9421 aatcacgcta aaggcataaa taattggcat aatatcaaaa ttaagttcgg ataaaatgag
     9481 caaaaatcca acaaaagcaa caaggccctt catcgacgag cgtgcaatcg gcacaatggt
     9541 ttgggtaaag ggattgggcc cttgcttttg cgcaccttcc tcttttgctt ggagttgaga
     9601 cgttgctata aaatcaaaaa gagcccaaag aatacgtaca cttataatga caaggagaat
     9661 ggaaagagct gtcagaatga gctctcttgt ttgaggtgtg tttaaaaggg tcaaaaaact
     9721 cagaccccat atttctaaaa tgattgcagc aatggcaaaa aaaagacaaa aatgtgctaa
     9781 ggggatgagg aaataagctg attgaacaac gggtaaggtt tttgcataac gccatggatg
     9841 gtgaatatcg aaggagggag agggtttgat gggaaaatct tgccagcttt ttgagagttt
     9901 ttgcccaatg agagagagtt gtgtgctgag aaaacgccaa agtgcaagaa aaatgaccgt
     9961 gaaaataatg gcttggccat agaataatgt ttcaaaaaga gcgccataaa tccaggcaag
    10021 gtagccgata aacagtagta aaatggctaa aatatgccag agacgcgcaa ccgcttgtgc
    10081 tatatttaaa agcacccctg caagatctgt gcgattctca aaatgcaacc atttagagac
    10141 ggggtggcga taatccacaa taagtgtggc taaaacaaga aaaagaccaa acccgacaag
    10201 gctcttccac gtttcacagg cttttggatt catgtgaata agctgaaaaa tctgcgcgca
    10261 aaagacccct aaaaaaagaa tctgcacaaa aaaactaatg aggtggaaaa aacgtcgtgc
    10321 attgcgatcg cttaacagta aaaggcgaag atctgaatgg tagggctgca aaaaaagcct
    10381 gaaatattga agcaatagac gtgataaaaa caaaatacct ataatcacat ggacggaatg
    10441 taaaattaca ggtcgctgga aaacaatcgc aaccgtatag gctgtaagtc caaaaagaat
    10501 aaggggaagt gtgctgatta aaaatgcgcg tgtgaattct ccccatgtgc gtgggggttc
    10561 tttgggcgcg cgcaatccaa ataaaacaaa acgaaggaga aattctgcgc caaaccctgt
    10621 ggcaagagag aggaagagaa ggagagagaa attgacaaaa tgattaaggg cgtcgctgtc
    10681 gctatcgttt aaattttgaa atccttgcca gacttcaaag ggaaactgag ccaaggattg
    10741 aatagcctga ataagctgct gtgcggattc ttcaatacgt gtgataagcc ctgcattgag
    10801 attatgaatc attgattgcg atgcattttt gctatcgacc tgattttcaa gggtgttcag
    10861 atgtttgaga gtgttcaaaa acgcttgacg cttttcatca tcttcaagaa tttgaatgac
    10921 tgcaactgtt tgcgcatctt tatccgcatt tttattggga tttgcataag cacataagca
    10981 aagagaaaga cagaaggccg ctatttttaa aaacatcatc atttaaaatt tagctaaatt
    11041 tatttgattc tatcaatttt tagtgcatct tccaagaaat gaaaaaaaga atcggagtcc
    11101 ctgaaagaca gcgtgatagg aagagtatac attttttctc tccatggggc ggggatttta
    11161 agataatctt tttccgttgt taggataggg cagctcaacg cgatactgcg tgtatatagg
    11221 tcaccgatgt ctttttctgt atacacataa tgatctggaa aatcataaaa tgcgaggatt
    11281 ttaaacccta aagtttcgag ggagtgttta aattttttgg ggtatccaat ccctgcaaaa
    11341 gcaataatcg cttgtggtgg gatgggcaaa cgtgcttcaa aatgtgcatc aaagacaggt
    11401 tttttataaa gagtcagtgc atctttatct gtttttgatc cgtgaataag aataatcgca
    11461 tcggcctctc tcatgccttt tttagggaaa gtccgcagag gtcctgctgg aaaagtacac
    11521 ccgttcccta ggccctgttg accatcaatg actaaaagtg tgagatcgtg aaaaagcgat
    11581 ggattttgca acccatcatc cataatgcac acatctgctc cctcatcatg tgctgctttt
    11641 cctgattgat agcgatcaga acaaacccac gttgttccat gcttggccaa aagcaagggc
    11701 tcatcgcccg tataggatgg attatctaaa agggtagttt tatgcaaatt tttgactttt
    11761 ccaccatagc cgcgtgtcag aaaatggggg gttttcccat agtgagtttt taaaatatcc
    11821 gcgatcgcaa tggctgtagg agttttaccc gatcctccga gcgttaaatt gcctatgcat
    11881 ataacaggga taggaagacg cttttgaaag cacttgcgat aaaaaaaatg aaaagtgctg
    11941 agcattgtat aaacaaagga aagaggggag agggcaaagg ccttccaccc ctttttttca
    12001 taccaaaatg cgggggtttt catgggaatc actcaaataa ggttgacaca gctgaaccaa
    12061 gtctttgaga ctatttttct gtatcgacat aaaattctta agcgcctctc ctttttcttg
    12121 cgcttcatgg gggtgagata aaaggtttgt gagaatttca gtcagattgt ttgctgtgac
    12181 ctctaggcta taagggccca taatctcaca gatatcttcc gcattgtgca taaagggtcc
    12241 ccataaaaca caacatccca actgcgcagg ctcaatggga ttatggcctc caatagcggg
    12301 aacggtgtat cgggtctcaa gcagtcaagc agccacgccg tcaccagcag gcgcgaactt
    12361 tccggcacgt tgaccagtgg ccacagcgca tcgagggtgc cgccctcaac cggctccggc
    12421 agcggcttc
//
LOCUS       DAIAHG010000015        11370 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_15_length_11370_cov_3.209953, whole genome shotgun sequence.
ACCESSION   DAIAHG010000015 DAIAHG010000000
VERSION     DAIAHG010000015.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11370)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15_length_11370_cov_3.209953"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    6607..6616
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3308 a         2493 c         2263 g         3296 t
ORIGIN      
        1 atgcacctca ccaagcaggc gattatgatt agggtgactc acacctttat aattaaaaac
       61 acgtgtttta aaaagcggat aatactctgg aaaatggtgg gaaagggctc ttgaaagaat
      121 cgccatatca cgtgccgttg tgagttggcg gctatcggga aggccgtgag gattataaaa
      181 aatggtgttc ttcatgccaa gcttacgtgc tgtcacattc attaattttg caaaattttc
      241 aacacttccg ctgaggccaa cagctactgt tacagcggcg tcattcgctg actttgtgat
      301 aagcccaagg ataatatgac gtatgagtat aatttgccct gttctgaggc caagtttaga
      361 cggctctaca ggcggtgcat tgcgcggaac aaccaagggt gtctccatgg atattttttt
      421 ctcacgtagc tttgaaaaaa taatatagag tgtcatcatt tttgtaagag aggctggaaa
      481 gcgtctgcca tcggcatcga tgtgatgcaa cacagcacct gtttctgcat ccataagaat
      541 ctcagcatat ttttgcgcat acgtaggaaa ggaaaagaga gtcgagaaaa aaaagtaaaa
      601 gaataagaaa tttttcatca atttttgtaa aataattata atgctctttc ttaaaaatat
      661 cctataaaaa aattaaaaac gcaatcatct atatcccttt cagcccctca cttctcgaaa
      721 atatttactt aatcttaata ttttttagct atctttggag tatgaggagg agaaggattt
      781 catgaccaca ccacattact atattattaa taaagccatt gcggctcatg gtgtctggaa
      841 aagtcgtctt attgaggcga tttcaacagg aaaatgtaat atcagtgcaa aaaatgcatt
      901 acgtgtggat gtttgtgacc ttgggaaatg gatttatggg gaaaatcttc ttttttttag
      961 aggcaaccct cagtacacgg ctttggaaaa ggtccatgct gaatttcatg cgcggacagg
     1021 agaaatttta aaacttgctg aaaatggaga gacagacaga gcctctgagc ttttggagcg
     1081 cgatcaacct tttgctcaaa catctgtgca acttgtcaag tgcctcaaag aatttggagc
     1141 atttttagaa gagcataact atgaaacaaa agaggatatc catttttctg tggagaccct
     1201 ctttggactt aaccctgatc aataactgcc taagcaagcg ctttttgaag atactcaaga
     1261 ccataaactc gttcaatagg gccaagggtt gcaagggtcg gcgccgatga aaaaaggcga
     1321 tcagcaagta ttttgatatg atcagccgta acactctcaa tttttttcaa agtctccgtt
     1381 gcctttagag gcgtaccgta agtgtgcatt tgattggcaa gttgctcaca acgagacgat
     1441 gtactttcta aactcatcat aagacttgcc ttcagctgtg cctttgcacg cacaagatct
     1501 tgctcaaaat gagtagaact aaaatgctta agctcactcg ctgcaacatc taaaagctca
     1561 ttcgcttttt cctcacttgt cccggcatac ataccaaaga tacctgcatc tgatgcgggc
     1621 gagtgaaacg tatagacaga gtagactaat ccgcgtttct cacgaatttc ttgaaaaagc
     1681 cgtgaggaca tgccgccacc aagaatcgtc gacaggaggg ctgctgcata gtaatcctca
     1741 tgtttgtgag gaacgccctg aaatcccatc accacatgca cctgttcaag atcgcgggtt
     1801 tggcgaaact ctcccccttt gtacgccgca acctctacct tgtgatgaca gtcttctttg
     1861 agatttgtaa aataatcttc tgccattgct acaaaatctt tgtgatgaat gtttccagcc
     1921 gctgctaaga ccatttgttt tgcaccataa tgattatgca tataggtttt gacgcgctct
     1981 gccttgaggg agctaacaat ctctgaggtg cctaaaatag ggcgccccat aggttggtgg
     2041 ggaaaacacg tttcttggaa ataatcaaaa ataatatcat ccggcgtgtc ataggtctgg
     2101 ccaatttctt gtaagataac ggattgttct cgttgaaatt catcctcttg aaacgttgaa
     2161 ttctgtaaaa tatccgccat gatatccacg cctaagcgtg cgtcattttt caaaaggcgt
     2221 gtgtaatacg cggttgtttc acggcttgta taggcattga catagccgcc gacattttca
     2281 atcgcctcag caatatcctt ggcagaacgc ttttttgttc ccttaaacgc catatgctcg
     2341 agcacatggg aaatgccatt aatttccttg ggttcattgc gcgcgcccac attcaaccaa
     2401 acaccaagag tgaccgtttc aacacttggg atatgatcac tgacaacgcg taaaccgttc
     2461 gaaagagttg tgatttgaat agacatttat gaatttcctt tttgtgtgag acctttatct
     2521 ttcgcatagg gcgagctcat gtgaagtttt gcaagcgcct catcattttc aacaatacga
     2581 atagtggagc ctactttgat cggttttgca aggtcaaacc cttttttaag gacttcaatt
     2641 accagatcgg ctgtatcata ccccatttgt gtgcgatcat aggccacagc ggcaagcgct
     2701 cctttttcca cagaagcact atcattagca aacacaggaa cgccctgact atgggcgaca
     2761 cccacgatcg ctgaaaccgc agccatagcg gtattatcgt taggaaaata aatcgcatca
     2821 accttcccca aaagattttt cgtcgcaggt accgcctcat tagtgctatt caaaggagat
     2881 cttacaatcg ttatccctga agccactgct gctttttcaa atgtctcaag ataagttgtt
     2941 gagttgactt cagacggatt atacaaaacg cccagcgttt taatagaggg caagaaggat
     3001 ttgatcattg caatttgcgg ctctggcggc atgtagtcac taatcccgtt aatcccaggc
     3061 gtttttgcgt ttaaattctt cacaagcttt gccgcaacag gatctgtcac agccgtaaac
     3121 acaaggggaa tatgctgttg ctggctaatc gtttctaaaa gttgtgcact ttgagtggag
     3181 agtgctacca gaaccttagg attaagatgg gcaaaatgct ggacaatctg agctgctgtt
     3241 gtcatttgcc cttgtgcatt ttgatagaca attttaacgg ttttgccgtc aataaaacca
     3301 cgctcggcaa ggcgtttaat catcccttcg cgtaccgtat caagggtggt atgctcaata
     3361 atttgggtaa tggcaatgag agggagcgcg tgttcttttt ttgaaaaata gtgatagaaa
     3421 aaaacggcta aaataagact aaaaaggata aaaccaatag atcgcttaaa cataagaatg
     3481 aacctttcag gataaaaaca gaatttaggg cgcaaaaaac tacaccccta acagcttcgt
     3541 ttttagaagg ttctacagca aaaaatatca atggatacaa aacgtctcat ggcatagatt
     3601 ttatccaaat atctcccttt gcgcaactta attttttgca tctcagcaat aaaacctcct
     3661 catcaaaata tttatttttg atataatctc atgaatgtag attaattttc ttaaatgtat
     3721 tattggagat tttttatgaa aaattttaaa aaaatagttg cggtcgcatc gctttttgtt
     3781 ataacgggga gcgtggcaag cgcgggcatt atccatttta cagagcaagg tggtgggggt
     3841 aacactccct tttgtgatac ggggggtaac tccgtctctt attacgaatt tccaaccctt
     3901 gataagaata agaatgaaat aaagaagatt agtgataact ccaaaatttc cagtctgcat
     3961 tatgatccgg ataatcaaaa aacaacagat aagctaaaaa attttatttc ttgtgaagaa
     4021 tctaaagcat tagatgcatc aacaacactc ctaaatcctt tctttttttc aaaggaaaca
     4081 gagactctca cgcacctttt ttcttatgtt gatcccactt ttatggctct tggtatctac
     4141 ctttttgcgg atcaaaagaa atatctcgca gatagtgaaa atgaaaatga ggagtctctg
     4201 ggggaggatt tcataatgca tgataaagat ataaataccg tggagaaaaa tctattggga
     4261 tacctcaaga gtgactctct taaggataat gccaatgtaa aagcgtataa tgctctcctt
     4321 gaaactctgc ttaaggggga tgccatcaaa agccgaaagg acgccgtgaa atttgttctt
     4381 gataagattg aaaaagaaag caattaaaaa aactaagaga gggtgcgact atagctgccc
     4441 tcttctccta aattttagta tcaggtaccg ttcaggaaat ttttttcata atcagcgatg
     4501 cattggttcc accaaaacca aaagaatttg acatcacaac attgacccct cgctctttgg
     4561 ccttgagggg gacaagatca aggtcacatc cttcatcggg ttcatgaagg tttaatgtcg
     4621 gcgggcagat attatcttga agcgctaaaa gactaaaaat cgcctccaca gaacccgctc
     4681 cacccagcaa atgtccaatg gcggattttg tcgatgacat ggataatttt tttgcatgat
     4741 ctccaaaaag acgttttaca gcgcgcactt caatagcatc gcccagaggt gttgacgttc
     4801 cgtgggcatt aatataatcc acagcctctg cgtccaattt tgcattttta agagcagccc
     4861 gcatggcacg aaatgcaccg tcaccatctt ctgcaggtgc tgtaatatga tgagcgtctc
     4921 ctgagagacc atagccgata atctcgccat agatctttgc acctcgtttt ttagcgtgtt
     4981 catactcctc aaggacaagt gcacccgcac cttcacccat cacaaagccg tcacgacctt
     5041 tatcccaggg gcgtgatgct gtggtaggcg catcattata cgaagtcgac aaggcgcgac
     5101 atgctgcaaa ccctgcaaca gcaatgcggc agagcgatgc ttccgcactt cccgcaatca
     5161 tcacgtcggc gtcgccaaat tgaataaggc gtgctgcatc cccaatcgca tgagcacctg
     5221 tcgcacaggc cgtcactaca gagtgattag gccccttaaa accatgacga atggtcacat
     5281 gtccagaaat taaattaatc agagaggctg gaataaaaaa cggactaacg cggcgagggc
     5341 ccgattcttt gagaatttga ctcgtacgat caatctccgt aaggccgcca atacccgaac
     5401 ctaaaagaac gccagtacgc tctaaagact ctgtatctga gggctgccac ccagcgtcag
     5461 caatcgcttg atcggcggca gcacatccaa aaataataaa tttatcaact ttacgctgtt
     5521 cctttggttc catataagct gatggattaa aaagcgcatc gccaaaatct gctgcttctg
     5581 atgttgggac agctccccca acgcgcgcag gaagatcgtc cacctgaaaa tgatcaatgg
     5641 cacgaatgcc tgactctcct tttaaaagac ggcgccacga atccttgaca tttccagcca
     5701 agggcgaaac catccctatt cctgtaacca caacgcgacg catacacatt tctcctaaac
     5761 attaaaaaaa tagccttaga gtccttagaa aaaggatgct aaagctatga tgctagagtt
     5821 ttattaagca gcttttttat gattttcgat ataatcaatt gcggacttga tcgtcgtaat
     5881 tttctcagcg gcatcatcag ggatctcaca gccgaattct tcttcgagct tcatcacaag
     5941 ctcaacttga tcaaggctat ccgcaccaag atcatcgaca aagctcgcat tttcaacaac
     6001 tttagccgca tccacaccaa gatgctcaat cacaatttct tttacacggg aagcaatttc
     6061 tgacatattt ttgaccttat taacagtttg ttaaatattt tatccctttt tagcacgatg
     6121 atcaaaagag tacaagaaaa tcatttccat ggcatcattc caggaaaatt tttgagcgtg
     6181 caggggaaga agtagggcgc cctatcacac cccattaaac acgcaaaatt tttttttcaa
     6241 gatgttttgc tcatgcaatc tcacacaacc ataaaaaaaa ccaggcacat gtcctggttt
     6301 tttagtccat ataaaatgga ggaaggctta gttaacaaca gtaatcgctg tacgttgttg
     6361 tgcccatgtg gcttcatcag cgccgccttg tactggtggt ttgtctttgc cataggaaat
     6421 ggtcttgaga cgatcagcag caacaccgtg ttttacaagt tctgcttttg cagcggcagc
     6481 acgcctgtta ccgagagcaa ggttgtactc gcggcttcca cgtgggtcgc agtgaccttc
     6541 gaccattgct gttgcttgtg gatacatttt gaaccaatct gcttgagctt tgattgttga
     6601 catctcnnnn nnnnnnagat ttgttcaatg caaagaatac gcgatccttt acgttttgtt
     6661 tgaaatcttc agcgcttcca ggaatcgcct tcgtggaaac gttgacttct tgtacatctt
     6721 cgccgctgtt ttggcaagca acgagagaaa gagctacgcc ggtcaaagcc agcaatttgt
     6781 aaatcatgtt agtacgcatg tttttcatcc tttaatggtt atacaagtgt tattaaacca
     6841 actaaaggtt aacaaagaat ttatttttac aaaatataaa acttgatgtg ccacacacat
     6901 cctttcgcac ctgtaggata aaagacacac ttcgttgatt tttattaaca ttttagaagg
     6961 cttgagagct ttttttctaa aaaattttct ttagcccaat gttattattt tttccaaagc
     7021 cctacaaaaa gtatcaattt cctcttccgt attgtagtag tgaacagaag ctcgcacaat
     7081 ctctctaagc cctcggtcat ccatatcaaa gcgcgcgtat tctgcaagag aaatcgatgt
     7141 gttgatcgaa tgggtgtgta aaaaagcttg cacctcttgc gctgattgtg tggcatggga
     7201 aaaggtgaca atgccacatt ttgtctgccc cttatcatga actgtgatgt gtggaagtgc
     7261 agataatttt gaacgtaaag aatggccaag cgcttgaacg cgtttttgaa tatgctcaac
     7321 accccaagaa tgcgcatagt ctgcagccac acctaagcca atttttgcag ccacattcga
     7381 ctcccacgtt tcaaagcgac gggcatccga gcgcagaata tagtgatttt tggatgtcca
     7441 ccgcgctgaa tgcagatcta aaaagggagg agagagcttt tcacggatat cttctcgcac
     7501 gtataaaaac cctgtgccac gaggacctct aagatatttt ctccccgtcg ccgtaaggac
     7561 atgacagtgc aatttttcaa catcaagaga catttgccct acggattgac acgcatccaa
     7621 aaaataaaaa acacctgcat cctttgcaat ttttcccacc tcttctgcgg gattgacaag
     7681 gccaccgttt gttggaatgt ggttgatgct aatgattttg acatgtttat caatacgatt
     7741 tttaagtttt ttgagggaaa tctggccatg ctcgtcatcg ggaatcacat caatcgtcac
     7801 accatatttt tgtgctacat gcatataagc aatcacattg ctggcgtact cagatgttgt
     7861 ggtaaggatt ttatcgcctg cctgaaaagg aaatccataa aacgccatat cccacgctgt
     7921 tgtggcactt gacatcacag caatatcttc aggttttgca tgaatgaggc gtgcaagaga
     7981 cctataaaca ctcgcaatcg cactttctgc agccgcttcg gcttcatagc ctcctatggt
     8041 attttctaac gttaagtggt tgataacagc ctgactgacg cattcgggct gaagagaagc
     8101 ccctgcatta ttaaaatgaa ttatattttt acaggctggc gtctctaaac gcgctttttg
     8161 aatatcaatc atttttctgg attcctttat gtaatttttc tgggctggat tttatttcca
     8221 gcgccatttt gttccctcta aactatcctc caagagcacc cctgcttcat caagctccaa
     8281 ccgcaacgca tccgcagcct taaaatcacg gtctttgcgc gcttgattac gacgttgaat
     8341 tagcccttca acagcctccg ccgaaagagt ttcttttttc acagattgca taaaccaatt
     8401 tttaggattt tgctctaaaa tacctaagaa atacgctgat ttttttaatt ttgctgctac
     8461 tgctgggtct tttgacttgt tatagtctgt cactaagttt tgcaaatgtg ttaaggcaag
     8521 tggcgtattc atatcatcca aaagggcttc catcacagag ctatcaggct ctacatgagg
     8581 gaggtcaacg gaaaaattac ctgcatcccc caacgcacgg taaaacttat caagagattg
     8641 tttggcctgc atcagttgat cttttgtaaa atccaagggc tgacgatagt gggatgctaa
     8701 aagcgcatag cgtatagttt caccctgatg ttcactcagc aaatcgcgca cagttaaaaa
     8761 attaccaaga gatttggaca ttttagagcc atccaccatc agatgaccat tgtgcagcca
     8821 atattttgcc attcgctctg ttccgtgggc tgcacaactc tgtgccactt cgttttcatg
     8881 atgaggaaaa acaagatcaa taccaccacc atgaatatca aactcttcgc ccaaatacgc
     8941 agcactcatg gcggagcact caatatgcca tccaggacgc cctcgtcccc aggggctatc
     9001 ccaacctggt tcatcctcat gcgaaggttt ccacagcaca aagtcaccag gatttctttt
     9061 ataaagagcg acttcgatgc gcgctcctgc aatcatctca tccaaagagc ggcgtgctaa
     9121 tgctccataa tttttatatt tccctgtctc aaaaagcaca tgacctgtct caggataagc
     9181 aaaatctttt ttaatcaact cttcgatcat atgaatcatt tgaggaatat gctctgtcgc
     9241 ccgtggctca cgcgtcggcg gcaaggcatt gagggcgttc atatcatcgt gataggcttc
     9301 tgttgtacga tctgtcagct ggcgtatgtt ttccttattt tcctttgctg ctgtattaat
     9361 tttatcatct acatccgtga tgttgcgcac atacactaca cgtttataaa ggtttttaag
     9421 caagcgaaaa agaacatcaa aaacaacgac agggcgggca ttcccgatat gggcaaagtc
     9481 gtacaccgtt gggccacaca cgtacatacg cacatagttc ggatcaaggg gctcaaaggg
     9541 ctctttttgt tttgaaagtg tattatagag cgcaagcggc ataagcgtgt tccttggaca
     9601 gtagatgctt taactcactt agattacctt tataaaaata agtgtgccag ccctgtgatt
     9661 cagcaccgtc aatatttgct tttgtatcat caatcaaaag aagctcgtga ggctttagat
     9721 taagatcttt ttcaatatat gtataaaatt ctttttcagg cttaatgtac cctatgtgac
     9781 aggaggaaaa acacttctta aagactgcac ccactaagtt cacaatatgt tttgtgcgat
     9841 agggatcttg gtttgtccct atataagcga ggatatgcaa agattttaca aaatctatca
     9901 tttcaagatc tacgtaacga tcgtgttcta gccagtaatt tatatagaca tcaggggtaa
     9961 ggccaagatc ttgaaaaaga gggtccttaa aaacggtggt aagatgttct tttgtatcaa
    10021 gctttcccgt cgtcacagca taccactctg cctctgggta aattttctcg aaatgttttt
    10081 tagtaagacc taaatcctct ttgatagacg aagaccaaag atagcggcca ttgacgtctt
    10141 gccctctaaa aagtacacca tcaacatcaa aaataatcgc ttttatcatt ttccccagct
    10201 gtccaacttt tttttctttc ccatagcaca aatcattaaa ataacacaga aaattaattt
    10261 tttgtaaact ttatgcacca taaaatagca actaaggggc ataatgagta tgccttttta
    10321 acatcgaaac ccaaggagat aaaaatgctc gatacaaaaa tcaaagtcat agccatagcc
    10381 actttaatga cctcatcact tgcagcgccc ctgttggcat ccgaagagaa taatgacaat
    10441 attaaagagc gtaaggaaaa tgtaaaaaac tttaatagag tgttgctccg cgatattctt
    10501 aaaaatgttt taaaagaggg aagcgtttca gactcaaagg atttttcaaa gctccttaaa
    10561 aaagcgttta aagaaattca agatcttgag atggataagg atgatctcaa acaggagctc
    10621 attccattta tgcaggatgt tgtggaccca caaagaatgt attcaggcaa aaaaatgtat
    10681 acaaaaaaga gagcctatac acctggtgct gacagcctga agaatgataa aaacaaaaaa
    10741 ctctattcaa aatttataca caatgtctat gaattaacac gtggagaata aaacaggcct
    10801 aggatgggaa aaatatgacg aaggagatca catggccgtt cgccgatctt ttcccttttc
    10861 tcctatgcct ctgtttaccc tgggttggcc ttacgagcct tccccctttc tatataggga
    10921 tttgggttga tgtagagctc acctccatcg cgcttaccct gttaagcagc cttttatgct
    10981 tttctctttt ttttcagaaa aactataaaa aatacgtaga ccatccgttt tttctatgtt
    11041 tggggggaat acttctgtgg caaacgctat gcatagcctc attttctagc cctggattgg
    11101 ctcttattgg caaccccatg aacggggagg ggctttcgac atgggtgagt ttttttctca
    11161 tcacactttt ttttgcgttc tttttacact cgaaatataa aaattttctt gttatctccc
    11221 tttgcttatc cgcgataacc ttcacaagtt tgagcttttt gggccacccg cagtttggga
    11281 tttacaaaaa ttgggcgtgg agtccttatg tttttccctc ctatttagct tttcttggat
    11341 tatgcgttct cagcttatct tttctttaca
//
LOCUS       DAIAHG010000016        11364 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_16_length_11364_cov_3.251528, whole genome shotgun sequence.
ACCESSION   DAIAHG010000016 DAIAHG010000000
VERSION     DAIAHG010000016.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 11364)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11364)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11364
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16_length_11364_cov_3.251528"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3119 a         2208 c         2759 g         3278 t
ORIGIN      
        1 gctatcatca ataaaaataa tgactttggg ctgaatgttc gccattttca tgaattccac
       61 aagggagact cctttatagg aatgaccttt ttcattattg acacttaaaa tacctgcaaa
      121 aaagacaggt gattgtcctt gtgtgttgcg aatggttgaa aggggcgtgt cgcttaaatg
      181 agggaacgca ccgctaaaat caatgccaag atttttgagt tctgtggtac gatgctgatc
      241 aaagcgtttg taggggccaa tgggccctgc ttgagcgcct gtacacgcga taatattgag
      301 gcctttttcc tgccatgctt ttatcatcgc aggggccgct tcatccacaa gttgtgaccc
      361 tttatccgcg acggaatagt tcagcacaag ataggtttgt gaggctggaa catctttgag
      421 aagcgttcgt agcacctgtc tgtagtgatt aatggttgcg agttggagcg agggattttg
      481 ggatcgcatg agagttgaat caatgtcaaa aacaataaga atatctttga tatcatagtg
      541 atccaaaaga tcatcaacga tgggggcaac ttcggccatg ctttgacact gctgaaaatg
      601 atgtgcacag accattgatg tgatgagaaa acaaaaagca gcgaggtggc gtagcataaa
      661 atccttttca gtttttttat aaaagcgcgc agagcaaaat cacgcgcact ttttaggctt
      721 cgttttcctt gagttttttc tccaaccagt gaatggtata gtcaccgctt tgaatatctt
      781 tttccttggc gaggcgctga tggaggggaa ttgttgtgga aatgccgtca atagcatact
      841 caaaaagagc acgctccaca cgtgtgaggc attcttcgcg cgtatctcca aaaacaatga
      901 gtttcgagac aagactgtcg tagtgaggag ggactgtgta gccatgataa agatgactgt
      961 caacgcgaat gccaatgccg ccgggggcgt gatagcctgt aactttgcca ggacttggaa
     1021 taaaggtaac agcatcttct gcattaatgc ggcactcaat cgcatgccct ttaatgttaa
     1081 tatcttcttg ggtgaaggaa agtttatgac cggctgcgac gcgaatttgc tcgcgcacaa
     1141 gatccacgcc tgtcaccatt tctgtaatag gatgctccac ttgaatacgt gtgttcattt
     1201 ccataaagaa aaattcacct ttttcaaaga gaaactcaag cgtgcctacg ccgcgatagc
     1261 ccattttttg catggcctga gtgacaatat cgccaaggcg ctcacggtct ttttgtgtca
     1321 agccgggaga aggcgcttct tcccatactt tttgatggcg acgctgaata gaacaatcgc
     1381 gctcgcctaa atgcacaaca ttgccatggg tatcacaaat aatttgcacc tcaatatgcc
     1441 ttggtgcacc gagatagcgc tccatataca cttcgtcatt gccaaaattg gccttggctt
     1501 cgatttttgc aaggcggaca ttcgcgagaa gttcgtcgtc gttgtgagca actttcatgc
     1561 ctttgccacc gccaccgctt gtggctttaa tgagaatagg atagccaatg gctttagcgg
     1621 cactgagggc ttcttcatcc gtggaaacgg aaccagcaga tccggggacg actggcacac
     1681 ccagggcaat cattgtgttt tttgcagaaa ttttatcgcc cataatggca atatgatggg
     1741 gggtggggcc aataaaagtc atcttatgct cttcgaccat cgcggcaaat ttctcatttt
     1801 ctgagagaaa accaacaccg gggtgaatcg cttctgcgcc tgtcacttca gcggcactaa
     1861 tgagggccgc catgttaagg taactatttt tagagggtgc tgagccaatg cacacactct
     1921 catctgcaag gcggacatgc atagaatcac tgtcggcggt ggagtgtacc gcaacggttt
     1981 taatccccat ttcccggcaa gcacgtaaaa tgcgcagggc gatctcgccg cgattggcga
     2041 tgagtatttt tttaaagagt ggtttttcgg tgtgagcagg cgttgtcatt cggtcataag
     2101 cttttaagcg caatgtatgc tttcttttta cgctttttct gcaatttatt caaaacctaa
     2161 ttttctcaag agattttcag gaggtttttc ttgtgaagga ttttagtcat taggtttatg
     2221 agactaaaat cctctcagga gctctcttac tctaaaaaat cttaagcaac cttttcctca
     2281 cgttcttttt ctgaaaaaga gagtgtgttg agcacggtat caatgctgtg gcgttgcgca
     2341 agcggaaggg gcgtgagagg caggcgcatt tcatgtgtgc aaagacctat tttgcttagc
     2401 gcatatttaa tcggaatagg attcgtatcg tggctaaggg ctttatccag aagaaggagt
     2461 tggtccctta gcattgcaaa cttttcttgg tcttgatctt tccacgcgta ataaagttcg
     2521 taagattgct gagggagcgc attggccgtg acagaaatac acccatcggc cccaagtgca
     2581 agggcgggtg caaacgaaga tgaatcaccg accaagatag aaaaaggttt tgtgtgtgga
     2641 attttctttt tctgtttgag agagtctatg ccaaggcgca ggtaagcaag gcgcgaaagg
     2701 tccgggtggg aatctttaac agcgacaata gaggggatac gcgcaagctc taaaataaga
     2761 tccacagaaa gatccacgcc aacacggctg ggattattat aaaggatgat cggaatggac
     2821 gtatgctctg caagcttttt aaagtgttca acaatgcctt cgggattggg tctgacataa
     2881 taaggaggaa taacgagtgc ggcactgcat ccaagatctt ctgcttgtct caaaagggca
     2941 agggattcat gggtgcttcc agcgctgcat ccggcaataa taggcacaaa ggcagaggcg
     3001 ttctgaagag ctatttttat aagattttca cgttcattca tggtcaacat ggacccttcg
     3061 ccggtggagc ccgcgacaac aatcccatgg gtttttccgt tgagctgata ctgaatcatt
     3121 tttttaaacg caggaagatc aacttcccct tgcaggaagg gggtcacact tgcaacaata
     3181 gaaccgaaga acataacgat aatcactatt cacccatgat tttttctaaa gtaagcgctg
     3241 ttcgtttttt tttaaagacc tatttttttg caggtattct cacagcagcg tcattggtac
     3301 aggcatgtca tattcaaaca accgttccag gacattggga gcggctaaaa acatcaaaag
     3361 aatgcgtgct cgccaaaaaa ttccttaaat ggaaagcgct tgaggatgat aatgcttctt
     3421 ttgaggcgat tacggatttc tttcatgcga atccctcgtg gccgaatcaa gttcttttaa
     3481 agcgcaaggc tgaacaagcc attcatgagg gcacatccaa agataaaata cgttattggt
     3541 ttcgaagaaa ttatccagtg acagcggcag gtgctatagc gtttgcaaag gttgcacaga
     3601 ctgaaaaaga tcgagagatt atagaatcga tttttccaac aatcgatttt tctgaaaaag
     3661 aattcaaaat tttcattcat gtggcgaagc atcttttaaa aaataaagat tatatcaacc
     3721 gttttgatga caggatgaaa agtgagaaat tagatgcagc acggatggtt ttgccgtatc
     3781 ttcccaaaga ttataaaaat gtagggattg agcgcctcag gcttgcgtca aaaagtcatc
     3841 tccgtacgaa aaatcccctt gaaaagcggc ttaatattca ctttcctggg tatctttggc
     3901 agtatgcttt gtatttgctg aaaacggaac gtgatgatga tctttttcga atgctgaatg
     3961 agaaagttgt tgtagctgca gaaacgcttg atccagaact ttggtggaca acaattcgtc
     4021 ggattcttgc aaggcgtatg ctcgataaaa agaaatataa agaagcgtat gatattgctg
     4081 cgcattctaa aacaacaaag gggacggagt tcacagaagc tcagtggctc aagggatgga
     4141 ttgatcttcg ttttttaaag cgccccaaag aggcatttca cgaatttcaa atcctctatg
     4201 atcgtgcgga aacaccgctc aatttaagta aagctgccta ttgggcagca agagctgctg
     4261 aagcccataa taataaagca gaaatgaaca aatggctcga gaaagcctca aagcatcttg
     4321 ctctttttta tggccagctt gcccatgata tgcttcaaaa accctttccc aaaaaagatt
     4381 ttgaaatatc accggctgaa aataaagcct ttaatgatat cgaaattgta cgcgtgattc
     4441 gactgctgca taaaattggt gagacagaaa tgagtgagcc ctttttttgg aagcttgcga
     4501 ttaatataac aaaaccggat gagcatgaac ttttgataaa acttgctgca gaggttgcag
     4561 gaccccatgc tgctgttcaa gtcactaaaa ttggtgcaaa acatgtgatg cccattatta
     4621 aagaagctta tcctcaggta gaaaaggcgc atatgcctgc gctttctaat gaatttgatg
     4681 tgaatattca cgcacttatt catgcgatta ttcgtcaaga aagtcgcttt aaatttcaaa
     4741 caacaagtcg taagggcgcc aaagggttga tgcagattct cgatggcaca gcccatcagg
     4801 tatcacgtgg tgcggggatt ccttatagca atatctatga ccccaaaagc aatattgccc
     4861 ttggggaagc gtatcttaaa agtcttttgc gtcagtataa aggatctttg attttaacca
     4921 tcgctgccta taatgcgggc cctaatcgtg tggataaatg ggtgcaacaa tttggctatc
     4981 cgggagaaaa agggggtgtg gagcctgtgg agtggattga acgtattcct tataaagaaa
     5041 cgcgttttta tgtacaccgt gttctcgaaa attattggct ctattcgatt atttttgatg
     5101 ggattcccat ttctttttgg tataaaaaac tctattagag atgagaaaaa atttcaaaga
     5161 cattgatgtt taaggctatt tttgagtgat taaggatttg ccctctattt attttacgca
     5221 ggatgtcgat aaaaatttcg atgtaaaagc gtctcaagcg ctgctgcagg agtgcaacaa
     5281 tcgaatgggt ctcaagggcg aatttaaaag gatcagtctg tacgcaaaac acagtaataa
     5341 aacggttgca ggggctatcg gttttgttca tggacaaatc atgtggatcg actcaattta
     5401 tgtggaagaa gcctttcaaa acaaaggatt gggagggcag ataatcagtg agctcactca
     5461 aatagctgag aaatataatc tgcgtgagat gcaattaaat acctattttc caagggcttt
     5521 tgcgtttttt aaaaaatatg gttttgagga ggtggctgct gtgcctggtt ggaaatacga
     5581 tataacgtgt tatttgatga gaaaaatcat atgaacaaaa agcgacacag cgtaagatga
     5641 gtttgcgatg ccattgtctt ataaactaac aacctttttt ctgattgttt ctgtcgcgat
     5701 ttttgaggca ggaaccaata tacttttgcc ggtgttgccc acacttcaag tgttttttga
     5761 aacggatgtt caaacggtcc aatcaacgtt aagtttttat ttgcttggat ttagtctctt
     5821 gggccttttt gcggggcctc tctctgatca acttgggcga aggacactta ttcttgccgg
     5881 gctcattctt ttttggtttg gaagtttctt aggcttttgg tgtgtgtcaa cgtctcttga
     5941 tattcttctt ttttcccgct ttttgcaagg tttaggggcg ggtgtctgta cagtaatttc
     6001 tattgcgatt atccgcgatt tatttgacac actgagagct gcacgaatgc tttcccttat
     6061 ggggatggtc acggccgttg cgcctatggt ctcccctctt ttgggaggag cgcttgcgca
     6121 tttttggcat tggacagaca tttttcttct catgttttta gtagcgtgtg tcctattaat
     6181 ggtttttacc ttttggggag aggaatcttt atacaagaca gagccttttc agatgaaaaa
     6241 attagtggcg gaatgccgtc aggtgttgcg gaataaaaca tctgtagggt ttattcttat
     6301 ttcatcctta gcgtatggat ctctttttgc atggatcgtc caggccccct tcttctttca
     6361 agagcatttt tttctgagtg atatccacta tgcgttttat gcggctttgg ggcctgccat
     6421 ttatattttt ggatccttta ctaactatct tttccttcca cgctatggaa ttttaaaact
     6481 tttatccttt ggtcttattt tgagttttct tgggtgtatg atgctgatgc tattgatcac
     6541 atctcattca gcggaccttt ggtcgtatat gtgtggcttt ctttttttta acggcggggt
     6601 gtcttttgtt tttgcgaatg cctgtgccgc agccatcgat agggcagggg actgcagggg
     6661 cacggcctcg gctattcttg caagctcaga gcaatttttt gcagctattg cagcattcat
     6721 tgtgggtttt ttcccccatc aaacactctt gcccgcgatt ttactcatga cttttagtgt
     6781 tttaggggcg ggttttattt atttaaaaat ggaccgagga gagtccttgg cgttgtaagt
     6841 cgatcaaaaa atagtctcat aaattttttg caaggtctca aggtcctcga tgctgacatg
     6901 ctcattaatt ttgtgggctt ggtccgttct aagaccgagt tcgaaaacag ggcagatgtc
     6961 tttaataaag cgcgcgtcag aggtgccacc gtgtgtgctc agctccacat ttttgcctgt
     7021 gagatgatca atcttttttg cataggaagc ggctatatgc gtcttacatc cttcgaaagc
     7081 ctcgccggtt gtgatcatgt ggagctcgta atcctctaaa agattggatg ccctatgttt
     7141 aaaataatgc tcaagggatt cacctgtgtg gtgcggatta aagcgcacat taaagcgtgc
     7201 tgtgacattg tctgggataa ggtttgttgt cgcattcccg acatcaatgc ttgtgatttg
     7261 aagtgtactt ttctcaaaaa tatcagtgcc ctgatcgagg ggttcttgca gggtttgtaa
     7321 aaaagtgaga agagagggaa tggggttttt ggcaaggttc tggtaggcga cgtgacccgc
     7381 aacgcctttg acctttaaaa gcgcatttaa actccctctt gcacctattt taatcgtatc
     7441 gcctatgctc gtgcgtgctg tgggctcacc cacaaggcaa atgtcgatgg tttcattgtg
     7501 tgcgcgcaac cactcaagga caacaggcgt gccataggtt gcatcgcctt cctcatcgga
     7561 ggttaaaaga agactaaaac tctcatcagg atgttttttt aaaaggacag gaagggcggc
     7621 taaataggct gcgatccctc ctttcatgtc cacagcacca cgcccataaa ggatattatg
     7681 atgaacttct gcggcaaagg gaggataggt ccacaaggcg ttatcaatgg ggggtacaac
     7741 atctgtgtgg cctgcaaaac aataattttt cccaccgcga tcgcattttg cataaaggtt
     7801 actcgtgccc tgtttgtcaa ggcgatgtga gataaaacca agaggggcaa gatacgcttc
     7861 gataaaatcc aagcatccat ggtcgtgggg tgtaatactt ggaaagcgta taagcgtttg
     7921 agcgaggaga agaacagagg atggcatgat ttttatgcgc gattttaaag gtcaaaataa
     7981 gcaagccaac acctgcttat tttaagggag cgttaaatcg ttaaaaagaa tgttttaggc
     8041 catattactt cctacaaaat cccaattcac taagtgatct aagaagatat taatatattg
     8101 agcgcgaaga ttgtgataat caatgtaata ggcatgctcc catacatcga tggtcaaaag
     8161 ggcttttgca ccatggacca tgggaaggtc agcattgcct gttttcataa tggagagctt
     8221 gccatctttg agcacaagcc atgcccagcc actgccgaac tgagtaaggg ctgcttgtgt
     8281 gaacgcttca cgaaaagctt cataggtgcc aaaatccctg tgaataagag caagtaaatc
     8341 accacctggt tgaccaccgc cattgggtct catgcaatgc cagtaaaatg tgtgattcca
     8401 cacctgggct gcattattaa aaatgctcgt gttcttttct ccaaaggacc tttgaatcac
     8461 ttcttccaag gaaagcgtgg cataagttgg atgatctttg agaagattat tcagatttgt
     8521 gacgtatgtt tggtgatgct ttccatggtg atattccaag gtttttcgcg aaatatgagg
     8581 ctcgagagcc gtcatttcat aaggaagttt ggggagttcg tgggccatga gcgcttctct
     8641 tgagtatcag taatgctcca cttagtagtc atgaatttga taaaaaatgc aacagcaaaa
     8701 gtgacactaa attttggaat ttctcccgaa aatcactcct catggtggag aaaagagttg
     8761 ttagtgtagg attattccca tatttctttt cgtgcgtgat acgattgatc ttgaatttta
     8821 agtcgtgaat ccttacattt tctctagaac attaaggttg ataatatctt ttagggtaat
     8881 tgattgaatt tatcggaaaa agttgaacat attttggctg catcgctgga gcatcaaggc
     8941 tatgcgattg tgcgtgtgca ggtttctgga aacgtgcgca agacagtaca aatcatgatt
     9001 gaaaagcttg atcatacaga gataacaatt gaggattgcg agcgcgtgag ccgtcacgct
     9061 tctgttcttt tggatgttga ggatcctttt ccttttgcgt atgttttaga agtatcatcg
     9121 ccagggctcg atcgtcctct tgtgaaacca aaagattttg agcgtttttc aggcaaagac
     9181 atcaagttac acttgacaga gatccttcat cataggaaaa gatttatagc acacctcaat
     9241 catgcaaatg atatggggat taatgtaacg gtgaaggaag aaagtgcaga agaggatctc
     9301 tcttttgatg ttttctatca tcaaatttta agcgcaaaat tgcacgttga ttttggtcat
     9361 tctataggaa aaaagggtaa agccagtggc acatcgaaag gttaaattac acgaacatat
     9421 ccaagcacag ccgcgtcatg agttgttgca agtggcagat gtagtcgcac gtgaaaaagg
     9481 tattgagcgt gaagagattc tttttgccat ggagcaggcg attttaaaaa cagcgaaaac
     9541 aaaatatggt ttcgagaacg atattcatgt ttttgtggat cgtacaacgg gtgctgtgac
     9601 gctcgaaaag catattacca ttgtggagga tgaggctttt gacaatgcga tgactcaaat
     9661 gaaattgagt gatgcacaaa aagatcctga gcttagcgat atggaggtcg gcgaggttat
     9721 tgtttatgat cttccgcctg tggagtttgg tcgcgtggcg gctcaaagtg cacgtcaggt
     9781 gattacgcaa aaagtacggg atgcagagcg tgctaagatg tacgaagaat ttgaagggcg
     9841 cgtaggtcaa atcgtgagtg ctgtggtgcg ccgcatagag tttggaaatg ttatccttga
     9901 gcttggtcgt ggtgaggcga ttatgcgccg cgatgacatg atcccgcgtg aaaattttca
     9961 atcgggcgac cgcatccgtg tttttattac cgaagtcaat cccaatgcac ggggtccgat
    10021 gatttccgta tcaaggactc atccatggtt tatggcaaag ctttttgagc aagaagttcc
    10081 tgaaatttat gaaggtgtga tcgaggttaa agcgattgcg cgtgatcccg gtagccgtgc
    10141 gaaaatggct gttttttcac cggattcagg tcttgatcct gtgggtgcat gcgttggtat
    10201 gcgcgggtca agggtgcaag ctattgtgag tgagctgcac ggagaaaaag tcgatattat
    10261 cccttggtca tcgaatcctg caacatttgt tgtgaatgcc cttgcgcctg cagaggcgtc
    10321 caaggttgtg attgatgagg aggctcatcg tgtggatgtg gtcgttcccg aagatcagct
    10381 gagccttgca attgggcggc gtggtcaaaa cgtgcgcctt gcgtcacagc tcacaggatg
    10441 gaatattgat attctcacag aggatcaaga aatagcacgc cgtacagaag aatataatct
    10501 gcgcacacag ctttttatgc aggcgctcga tgtggatgaa acgattgctc agcttttagc
    10561 ggcagaaggt tttgtgagta ttgaagaact tgctgaggtg gctcttagtg aatttgcgcg
    10621 tattgaggga tttgatgaag agattggtga agagcttcaa aatcgtgcta ataactatct
    10681 ccaaagcaaa aacaaagagc atacaactgc ggcaaaaaaa ctaggggcat cggacgatct
    10741 cctggcactt gaagggcttt caccggtttg tcttattcag cttgtggaaa agggcattaa
    10801 aacgctcgat gattttgcgg atttgtccgg ggatgaagtc cttgatattt tagggcgaga
    10861 tgctatgact cttgagaaag ccaatgaact tattatgaaa gctcgtgagc actggtttag
    10921 tgagaaatag ttttaagaat acactttaaa gaaagcacgc catgacggac gataccccaa
    10981 agcaaacaaa aacccttacg ctgggtaaga aacttgaatt aaaaaaacct gtggatatgg
    11041 agcaggtcag gcagagtttt gcccatgggc gatctaaaac agtggctgtg gaggtaaaga
    11101 aaaaacgtct tacagcagcg gagcaagagc agaaaaatca agaaaattct ttggaggatg
    11161 cagctgcttt ctcccgcttt acaaaagatg agcttgagtc tcggatgcgc gcgctgaagg
    11221 agtcaatgaa agaaaaacct aaatccttcg aggaagaggg ggactatcat gagcaagagg
    11281 gacaagcgga cattccttct gaactatctt ctggagctgt tgaggcgaag gaaacctctt
    11341 caaaagaacc ttctttggag gaaa
//
LOCUS       DAIAHG010000017        10984 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_17_length_10984_cov_3.224810, whole genome shotgun sequence.
ACCESSION   DAIAHG010000017 DAIAHG010000000
VERSION     DAIAHG010000017.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 10984)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10984)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10984
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17_length_10984_cov_3.224810"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3315 a         2379 c         2241 g         3049 t
ORIGIN      
        1 aatgattgag cttcacctgc aaaagtgtgc gcgcttctgg atccaatgtc gtctgccaca
       61 actgctctgg attcatctca ccaagacctt tgtagcggct cactgtcatc cccttgcgcc
      121 cttgatccag caaaaattca ccaaaggttg agggaccgtg gaaatgacta tcactatttt
      181 tatacatcaa gcttaaggaa aaaatattct taaaccgtgg ttgatacacc tcaaactgac
      241 gcacctcatg gagatcaaaa aaagcatcat caatagggaa aagttcactc actccaaggg
      301 catgacggct aaagaggcgg tgattttctg tacgctccat cgaccatttg acctgaggct
      361 caacggcatt caaacaattc acaaccttca tcagcgcttc atctgaggaa ctaaccttta
      421 tatcatttaa aagaagttgt tcaattagca cggtcgaact gatgcgttta taaagtttcg
      481 cacacacatc ctttgcttta aggcaaatgc gcccaaaatc cttcaaatcc tccccagaca
      541 tttgatgccc ttcggatgtt gtcacaaccc cctcatgggc tatggtttcc aaaagatagt
      601 tgcgcagagc ctcatcgtct ttaagataaa cctcagactg gccacgcttc actttataaa
      661 gaggtggctg tgcaatataa aggtgcccac gttcaatcaa cggacgcatt tgacggaaaa
      721 agaatgtgag taagagtgtg cgaatatggc taccgtcaac gtccgcgtcc gtcataataa
      781 taattttatg atagcgaagt ttgtctggat taaattcctc tgaaccaatc cctgtgccaa
      841 gggctgtgat aagtgtgcca atttctgcag agcccagcat acgatcaaag cgagcccgct
      901 caacgttaag aatttttcct ttaataggaa gaatcgcctg gcagggataa gaacggttgc
      961 gcgcttgctt agctgaacct cccgcagatt caccctcaac aataaaaagt tcgctttttg
     1021 caggatcacg ttcttgacaa tcggcaagtt ttccaggaag cgacgcgatg tccagcgccc
     1081 ctttacgccg tgtgagttcg cgggctctgc gcgccgcctc acgcgccata gctgcctcta
     1141 aaaccttgct aataagtgtt ttaataatgg caggattttc ctcaaaccac tgttccaaag
     1201 attctgacac acacgactca acaacagcac gcacctctga agaaacaagt ttatcttttg
     1261 tttgcgaaga aaatttagga tcggggacct ttacagaaag aacacaggtc aagccttcgc
     1321 gtgcatcgtc gcctgtaatc gttacttttt cttttttccc aagctctgca acatagtgcc
     1381 ccataatgcg cgtaagagcc gccctaaacc ctgctaaatg cgtaccccca tcgcgctgag
     1441 gaatattatt cgtaaaacac aacatcgttt catgatagct atccgtccac tccagtgcgc
     1501 actcaaccgt tgtttgatct ttttgcaacg aaaaactaat caccttgggt tgtagagaat
     1561 tttttccctt atccaggtat ttaacaaatt caacaaggcc accttcataa tgtaaaatcg
     1621 actcataagg cgtctcacca cggttatctt gaaaacaaat aaaaacacct gaatttaaaa
     1681 aggcaagctc tcgtagtctg tgttctatcg ttgcgcgatt gaattcaata ttcgtaaaag
     1741 tttggcgaga gggataaaat gtgacttccg tccctttgcg atctgtgctg ccgacaactt
     1801 tcagcggtgc atctgcgacg ccatctgtaa aagacatttc atgttcatgt cctgctctaa
     1861 agatacgtaa aataagtttc gaggaaagtg cattgacgac agatacaccc acaccatgca
     1921 aaccgcccga tactttatag gaattttgat caaacttacc ccccgcatgg agctgtgtca
     1981 taataacttc tgctgcggac acaccctctt catgcatatc ggtagggata ccacgcccat
     2041 tatctcttac agttacagaa ccatctgcat taataataac ctctatccgg tcacaatgcc
     2101 ccgcaagcgc ctcatcaatg gcgttatcca caacctcata caccatatga tgaagacctg
     2161 tgccatcatc cgtatccccg atgtacattc ctggacgctt gcgcacagcc tcaagccctt
     2221 tgagaacttt aatcgaatcg gctgagtaat ttccagagac agtggtcgtc atgaatggtc
     2281 cgtaatattt aaatctgact aggatctttt tagcacattt taaggagctt gaggggatga
     2341 aatttaaaaa ttaagaaggg aaaaatatct tgacactcaa aaagcatctg tggcgtttta
     2401 taaacgttaa tcaataataa aggaaaaaaa atagtgcatc atttattcaa taaaatactt
     2461 ttagcgatgt cactcactct tgcgatcaca acatcgagtg atgcaataga taaagaaaaa
     2521 atatgggtca agcacacgca tccactcaca aaaaaaattg aatctctttt aaatcaacat
     2581 gcgctcataa caaaaaacga cttaaagaat tttttaaaaa acaatagttc agaaattgat
     2641 agtgaaatca aggggattat aaaaaaatct ctcaagtata cgcctaaaaa gattgatgaa
     2701 gaagatgaat ttctctatct aaaaatgctg cctacgctta tgttcggcaa aaaaaatcct
     2761 tttgaaatgg cggaggattt ttatgactac tcgagtatgt ttgatattcc gctcaaaaag
     2821 cttaaggcaa catatataaa aaatcttcgt aataaaaatg aatgcctcgt tggtaaatct
     2881 ttgctggaag aagatttctc ggaggaatcg tggaaaaagt ataaaaatta cacgctgagt
     2941 caatatttac agcacctaaa gattgattcc caaatcatta agctctaaaa actgcgcttg
     3001 atgctgaagg ggagcaaatg catggacaca gctccccgtc atccacactt ggctcttacg
     3061 cccctgtcgc aaggacatca catcctctaa caatacaccc cggtgctggg aatcaagatg
     3121 atccatcaca tcatctaata ataacaaaag gcccacagaa tgggggctga caacaggtat
     3181 tttgacaaac gctaaaatca tagcaaggac aagcattttt tgctcacctg tcgagcaaaa
     3241 gcgtgcgggc atctgttttg ccgtgtgcgt cacgacaagt tttgagcggt gggggccgat
     3301 gcgtgttgtg ccttttattt tatccaacgg ccgactccgc agatattgct ctcgcaatgc
     3361 attgacacat gcttcttttg agcgaatatg cgtataaagc gtgtcgcttt ctccttccat
     3421 ttgacaagaa aatcttggaa aaagaggcgt tgtcgactgc ttttcttcaa gctgtgccag
     3481 aatatgatga cggctgtgtg tcagatcata actgagatcg gcaagtttac tttcaatggg
     3541 atcaagccat tccttataag tgcttggagg ataatctttt aaaatagcat tacgctcacg
     3601 cagaagtttg tcatacattg tgcgtttttt ggcgtgaatg ggctcaaaag aaaaaacaag
     3661 gcgatcaata aattggcgct gcgcctggct cccatcgcta agcaaactcc ccattaaggg
     3721 agtgaaccac acaacactta aatagtcggt aagacaggct tggtttttga gaatctctcc
     3781 ctcatgtttc acaagacgtc tctccgtttg catttctgct tgacgctgtg taccaaggct
     3841 gatgggaagg ccattatcct caagagctat gcccacttgc cagggatgct gatgcgcgtg
     3901 ctgctgcatc tctgcacatg ttgcggaccg tagccctctt ccgggcgaaa aaagagacag
     3961 ggcctctaag atatttgttt tgcccgatcc atttggtgcc gtaaggacaa caggcgcttg
     4021 agataacgaa agattaagcg ctcgaaagct ccgaaatcca tccaccatca aggagcgcac
     4081 accaaaacaa ggagttgttg gctgaaaaga ttgctggaat gctgtttgtg tcatgtagat
     4141 ggtattaaaa ttaggtgaga aatctaaaaa aacttaattt ttttttctat tactcttgag
     4201 agaaagacca ttttatctcc ttaggcgtgt tggcgttgag ctcaaaaaga gcaaagatca
     4261 cattatcccc ctctcctcca agagccattg ttgtatcaac aataatttca tcaatataag
     4321 aaaattgtag gcggtatggc tgacctttca caggaatcag tgtcaccccc tgattttcac
     4381 taagaagttc tattgtaaaa gcagagtcaa tgataaagcg tagcgcccca taggcctttt
     4441 gattcacacg aattttttca cacacacgca agtcaacagc ctctgaaagg tacatttccc
     4501 gtgtatgaag gtgattaata tcatgaattt ttgcctcgat gaaaacatgc tctggatcta
     4561 tttttttgta aacgtgggcc atagagcgac tctgaaaagg gcggcctttt tgattttcaa
     4621 ggatcatatc acacaattta atgagacggt ccttgccgtt ggaccattca cactgcaaat
     4681 ttccagagga ttgcgccgga aaaggatgat agcgcatact gaataaaaga atattatgcc
     4741 caacttggaa tctttctaaa cccgcttctt ctgatttttg aggatggcgt cccttgacgt
     4801 ccgaaagtgc taacacggaa tccaccatag aagaggaata gtggctctca cctcccacaa
     4861 gagaggataa tcttccatca ccgtgcctga aaaaacgtaa aggagggacc atttttgtca
     4921 tggtatgctg cgcttttgat gcagcctgag cataatgtgt aacacgcata aaattacgca
     4981 gctcgataag atgtttgatt acagtcagct gatgatccgg ttcacacgtc acaaaaaaac
     5041 catctctaaa aagattttgt tgaaggtgat gattaaattt ttgaagaaca tcattatatt
     5101 ttttatccaa gggaagatta taggccagaa gacaaagaat aaggccctta agtgctgtaa
     5161 gagactcata agatgggtcc agatgcagtg ccgctgtttg caaaaatcga aactgatgat
     5221 aaaaactttt gtaaaaaaca ttgagaaaat catcgtcagc actttggcca aaaaactcaa
     5281 aatgatgcat ccaagaagac agacgttcgc caactacgtg aggttgccac gcaagatgct
     5341 gataatgacg gtagtgacga atccaattta gaatactgcg ccgcataaac cgtcggcttt
     5401 ggttatctcc cacagcgcgc aaatcggaca accacgtaaa agcatgaaaa tagaccgaat
     5461 ctttcacaga ggcatcctcc cacatatttt tagcatgaga atcatcgcct atgccctgaa
     5521 agcgttctaa aagcctgtga gcttttgtaa agtcacctac ccagggatgg ttaggaatat
     5581 gatagaatgt aggggtgttc agagaaagac tgcgccataa aaaaatcagc tgatttttgt
     5641 tgtaataatc tttcagctga gaaaaaaaag ataatgggaa aagcgccatg aataaaatta
     5701 taaattatga gattgtaatt gctgtatggc tttaatatag ctggtttgtt taaaaatagc
     5761 actgcccgcc acaagaacat tggcacctgc ttgaataatc cgaggagctg tttggagatt
     5821 gacaccacca tccacggcaa gatcacatga tagatttttc tgctctaaat aatttcgcac
     5881 tttttcaata cgctcaagag acgagggaat aaagtcttgc cccccaaagc ccggattaac
     5941 agacataata agaatataat cgaccatatc aatcactgga tacagaaatt ctaaaggagc
     6001 ccccggatta agcgctatac ccgtccggag ttgacgccct ttaatgagag aaaggctgcg
     6061 gtggagatga tctgtacttt cggggtgaat aataattgtg tcagcgcttg ctgaaaacat
     6121 actaataaaa tcatcacaat tcttaaccat caaatgcact tcaaagggca gtgtggaatg
     6181 ccttttcagc atattaatcg tatgaggacc aaaactcata ttcggcacaa aatggccatc
     6241 cataatatca atatgtaatc ggtttgcgcc cgctgattga acagctttaa tctcctccac
     6301 aagattacaa ggattcgccg caagaataga tgctgagacc tcaacgactt ccgctttatt
     6361 cgacatgatt tttgtgccat ttttagaaaa aataatccct gtatatgaca ttagagattt
     6421 ttgatggatt tttcatctgc aaaaaaagaa tatgcacaac tcaaaagact tattgagcat
     6481 cataactatc tctatcatac ccttgatact cctgaaatca ctgacgcaga gtttgatgcc
     6541 cttgtcctta agcggaaaaa gattgaagaa ctttaccctg agttagttga ggggccggag
     6601 tctcccggtg cacccgttct taaaattttt aaaaaagtgg cccacaaaac gcctatgttg
     6661 tctcttgata atgctttttc aaaagaagat ttgcaggatt ttatagagcg catgaatcgt
     6721 tttttaaacg tacgttccga tcattttaat gacctttttg cggaacctaa aatcgatggc
     6781 ctttcagctt ctcttacata cgaaaaaggt cttttggtgc gtggcgctac gagaggaaat
     6841 ggaaaagagg gcgaggatat tacactcaat gtgcgcacaa ttgcagatat cccccgtcag
     6901 ctcagtgagc ctctctcttt tgaagtgcgt ggtgaaattt atatgacact gcacgatttt
     6961 catgagctca ataaaaatag agaagaccat cacgagccgc tttttgctaa cccgcgtaat
     7021 gctgcggcag gatctgtacg gcaactggat ccagggatta caaaggcgcg ctcgctgcat
     7081 ttttttgcct atcaggcttt ggatcttaaa gaaaaaataa caacacaaaa agatgcgatt
     7141 cattttctca aaacccatgg atttcacaca aatccccacg gtcgcctttg ccattcactg
     7201 gacgatctga tgttgtttta caaagaactt gaggaaaagc gtgaccacct tgattatgaa
     7261 attgatggct gtgtctataa agtgaatgat tttagtttgc agcaacgctt aggctttgtt
     7321 ggaaaggcgc cacgttttgc gattgcccat aaatttaaag cggaaaaagc acaaagtgtg
     7381 attgacacta ttcttttgca agtcgggcgt acaggcacga tcacacctgt agctatattg
     7441 cgaccaacgc ttgtgggggg cgttgttgtc tcgcgctcca cactgcataa ttttgatgag
     7501 ataaaacgca aagatatacg tcttcacgac catgtgtgga tccaacgtgc cggcgatgtg
     7561 atccctcagg tcgtaggcgt tattgtagaa aagcgtccca aggatagctt agaaattgtt
     7621 cccccgacgc actgccctgt gtgtgaaaca gctcttgtga tcgagggtgt ttactatgta
     7681 tgcccgaata cgcatggatg caaagcacag gtccttgagc gccttattca ttttgtcagc
     7741 aaagatgctt tgaatatcac aggccttggc gcaaagcata ttgctttttt ctatgagcag
     7801 aatttagtca gggagcctgc agattttttt acactcgaag agcgcaatcg tatgtccctc
     7861 acacctctga gtgcgcatga gggatggggc aagcagtcga gtgataattt atttaaagct
     7921 attcgtgagc gccgcacagt gcctcttgca cgttttattt acagcttagg gattcttcaa
     7981 gtaggcaagg gcattgcaga acttttggca cagcattatc aagcctctga tcattttatc
     8041 caagcgatgc tgcatcatcc ctttgatgaa gtccataaag atcttttgtc gattgatggt
     8101 attggggaaa gtgtcatccg cgatctccat acctatttta gtgatccttt gtcgcgaaaa
     8161 aatatcgaaa atctcctgcc cttcatgacg attgagccct atcaaagacc tcagcaacat
     8221 acatcacaac tgacaggaaa aacgcttgtg tttacaggaa cattgggtct cagccgtcaa
     8281 gaagccaaag aattgagtct gcaacatggc gcaaaagtga gtggcacgct ctcctccaag
     8341 acagattatc ttgtggtcgg cgatgatgcg ggaagcaaat taaaaaaagc aaaagaactt
     8401 ggggtaaccc ttttaacaga aaaagaatgg cttcaaatgc ttacttaatg tttttaatac
     8461 cccattatct cctctgtttt tcaaaaaagg ccaagaattt ccttgaaaag acaacccgct
     8521 ttcgtttata aatatccata aaaaactatt ggagaataac tcatggccct taaaattcgc
     8581 cttgcacgtg gtggctcaaa aaagcgccct ttttatcgta ttgttgttgc tgaagcgtct
     8641 gcgccccgcg atggtcgctt tgttgaacgc gttggctctt ataaccctat tttgccgtcg
     8701 gataatccag agcgcatcgt tcttgaggcc gagcgcatta aatactggct gagtgttggt
     8761 gcacaagcca cagatcgcgt tgtgcgtttc ctcggtcaag cagggattat gcctatgccc
     8821 gagcaaggaa ataatcctca aaagtctcaa ccccgtaaaa aagctcaaga gcgcatgaag
     8881 gaagaagcag caaaagcaga agcggcaaag gctgcagaag cagaagccta aaaaaaattg
     8941 agtgatatca ttcacattgc aacgatcggc gcaccctttg gggtgcgtgg tcatttaaga
     9001 cttaaaacaa atacagaaaa cccaaaaaac gcactgtcct acggttcttt gactgatcat
     9061 aaggggcgtg cttacacatt caaactcatt cgtgtggaaa atgataatac actgatcgta
     9121 agtgaagaaa gcaccctcga tcgcacggct gctgagggct tgcgtggcat aaaactcttt
     9181 gttcagaaaa aagatctccc tacgctaaaa aatgaggatg agttttatac gaatgatctc
     9241 ataggtatga cggcctatag tttagaaaat gagttactag gaaatgtctt agaagttcat
     9301 aattatggtg cgagtgatat tttaacgctt cgtatgaccg atggcacctt aaagcaaatt
     9361 gcttttattc gtgattctgt tcttaccgtc gatcaccttc agaaaaaaat tacccttgtg
     9421 aaggaacatc ttctttagtt catatttttt aaataaagtg gtctagtgac tttaatgatt
     9481 ttttgctagc cttaatcaca agtgttaata gcgaggtcaa aaaaatctgt ggagccccct
     9541 attcaccatg ttgtcctctt gacacttcct gtttttaagg gggtttttta tcaatttctt
     9601 gaaaaacatg gtaatgatat cgcctacaat tttgtttttg attatgatga gctgaagact
     9661 gtcacagaaa aaaatagaaa tgcccctgcc ctccttatct cctttggtac aggggttatt
     9721 gttcctgaaa aaattcttaa aaaattccat agagcgtata attttcaccc agcccctcca
     9781 gaatatcctg gtcttgatcc ccattttttt gccgtttttg atcgtgtcac aacctatggc
     9841 acgacgtgtc acatcatgac ggaaaaggtt gatagcggac ctattgtagc ggtgaagcgt
     9901 tttcctgttg agggcagccc ccatgccatg aaacttttgg atatgacgct gaaccatacc
     9961 tttgagatga ttaatcatgt ttttcaaaaa atccttgcgg agggtatttt tccagagccg
    10021 attgatgaaa agtgggggca agtgatctgc acaaaagcgc ttaaaaaatc actgtgcaag
    10081 gttgatcccc ttatagaaga agaggccttc tgtcaccttc ttcacagctt tcaaacaggc
    10141 gtgggagagc aaaaaaatct ttacattgac ctccatggcc atcgttttcg ctatgaagga
    10201 aaaatttagt tttttcatga aattccagat tttttaggca aaattgaagc attttttgtc
    10261 ggttttcatg aaaatttggt gattttcatg aaaaatacag aataattttg ctttttgaaa
    10321 gatttttgat cgtaatagtg gcatatttga gttgtctcac atgagaagta cgcgtttttt
    10381 gagcgatttt tccctttttt acatgaaaaa ccacaaaata tcggtcaaaa aagcgaaaat
    10441 tcgcctcaaa atcctaaaaa ttcgcctaac cacgcaaaaa ctacactatt tttcatgaaa
    10501 taccctcgaa atttcaaaaa atgtgtcgtg aaaatccatt tctgagagaa aaaaggggct
    10561 gtttgcaaag aaaaaagggg ctatccccac tcaaatcagg catctcaaga caagaatttc
    10621 tttctggaca tgaattgatg atttttgcta ctatagcatc tagccttgtt aaggcttagg
    10681 cttttatttt ttggaggatc aggtatgttt cgatttagca ccgcgtattg tttgaagcat
    10741 tttggcgtat tttccgccgt cacctttatc caccacaaaa attcacgaaa gcgacagtcc
    10801 tcatgaaaga ttctcaaaaa gctaaaaaac ttcttacgct tactcttgcc tctcttctga
    10861 ttcttactgg ttgtcaaaag caacacgttc ctaaccttaa aaccattgct ctcacacaaa
    10921 ttattgaaca caaagccatt gacgctgagc gtgaggggtt cattgcaggg ttaaaagagg
    10981 cagg
//
LOCUS       DAIAHG010000018        10833 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_18_length_10833_cov_3.470249, whole genome shotgun sequence.
ACCESSION   DAIAHG010000018 DAIAHG010000000
VERSION     DAIAHG010000018.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 10833)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10833)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10833
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18_length_10833_cov_3.470249"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    295..304
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2982 a         2178 c         2578 g         3085 t
ORIGIN      
        1 aaagcttgca atctggctca ctgaaaaggg ctatgacgtt gttgtgattg ggggaaagga
       61 tgatgatcac gcactccttc gggcgaatga aaaaattttt gatcttaccg gcaagacgtc
      121 tcttgagcag gttattttct taagtcaaaa tgcggccttc gctgtgggaa atgacacagg
      181 gcccattcat atggcggcgg caagtctgtg tccggtgttt gttctttttt caaaaaaagc
      241 accacctgcg gagcaagtgg gcgcgcgtgg gccactttat taccataaaa cagcnnnnnn
      301 nnnngaattt gacttttgag gatataaaag ctgagcttga attgtttatt ttaaaaacaa
      361 gcaaaataga gtagaaaaat ttcctttttt gttgcaaaaa gagtaattat aagaacaaaa
      421 aaaggatttt aaacatgaaa aaactatttt ttttactagt cgcagcactt tctgggcaaa
      481 cgctctcttc ttccgccttt gcttcggaag agatcattat taaagaaaat aaagaagata
      541 atacaaaata tgtctttatg attatgaaca acgaaggtcc caatgcgtcc ttcgaagatg
      601 gaataaaaat tgaaaactct cttacagtcg agaaaactga caaagggatt cagataagta
      661 actttaatct tgatagtaag ggtaaaaagt acaaagtttt agaacagcaa aaaaactttg
      721 taatagaaaa atcgaactcc taggcttttg gatttggtga aaagcaaatt ggaaaattta
      781 ttttagatgt gttgcttgct aaagatacag tgttgactct caatcctctg gaaagtgacg
      841 aggaagatag tgacaagggg cagcagtatg gtgcaaaagt gacctgggac tcgggtaatg
      901 aagagaacaa aatgcatatt cagtttattc ctgaggataa atacttttat gggttgtttg
      961 ttaagtatcc ttatgggctg aactatgagg ggagtattgc aacagcatat ccttgcttta
     1021 gattccgcct tattaatacg caatttggtg aaaaaaacta acttttttta ataataatat
     1081 tttaaacgat aactgggcgt gagtgatctt gcgccccctt tttttctgaa aattttcctc
     1141 tcaaaagcca aaagcgaatt cttacaaaag ttaataattt gttaatgtga tttgaaaaag
     1201 ctgtgctata aaagaaacag atttttttct caaaatgcgt ttttttatgc tttcccgcct
     1261 tcttggaatt ctttctgcag acatggccat tgatctcggc acggcgaata ccctcgtata
     1321 tgtcaagggc cgtggaattg ttttggatga accatcggtt gttgcgattt cgaatcaaaa
     1381 aggcaaaaaa caggttcttg ccgtgggcga tgaggccaag ctgatggtgg ggcgcacccc
     1441 tggaaatatt caggcgattc ggcctttgcg tgatggtgtg atcgcggatt ttgaggtggc
     1501 agaggagatg atcaagcact ttattcgcaa agtgcacaat cgtcatcgtt ttgttgcgcc
     1561 tcagattatt atttgtgtgc cttctggctc tacagctgtt gagcgccgtg caattcaaga
     1621 gtctgcagaa tccgcaggtg ggcgccgtgt attcctcatc gaagagccta tggccgctgc
     1681 aattggcgcg gggcttcctg tgacggaacc cacaggctcg atggttgtgg atattggcgg
     1741 tggcacaaca gaagttgcga ttttgtcgtt ggggggcatt gtgtatgcgt gctctgtccg
     1801 cgtgggcggt gacaaaatgg atgatgccat cattagttat atccgtcgca atcataattt
     1861 gctgattggt gagagtacgg ctgaaaaaat taaaaaagaa attggctccg caacaattaa
     1921 tgatgacagc gaacatatca caatgtcgat taagggccgc agccttcaaa acggtgttcc
     1981 tcaagagata acgattaatc aaaaacaggt tgctgaatcc cttcatgagg ttgtggcggc
     2041 gattgtggaa ggtgtgaaaa cagcactgga aaatacagcg ccagagcttt ctgcagatat
     2101 cgtggatcgt ggtatcgtta tgacaggcgg tggggccatg ttagcgaatc ttgaccatgt
     2161 tctttctgat gcgacggggc tttcggtgtt tgtggcagat gagcccctca catgtgttgt
     2221 gcgtgggacg ggaaaagctt tggatgagat gaatgtgctt aaagatgttt tagtgtcaat
     2281 gtattagtca tcaaaggtaa agggaggttg caatgatttt aaatgcactc ggactcaagg
     2341 ctgtagggct taagcgtcgt cttcctttgg tttctttttt gacatggttt ttagggaaat
     2401 ctgttcgttc tcagcatttc cgcaagccaa agccgattca tttgattttt ttagtagcct
     2461 ctctttttct tttaatttac ggcaatagca aacagtggcg gcctattctt gcactcgaat
     2521 ctgcttttct tgatttttca gcccctctta ttggaagatt gcagcaatct tttcattttt
     2581 ttgcagagat tgaatctttc tttcgctctc atgcttcctt gcgtcaagat cttgacagtg
     2641 caagaaggga aaataacaat cttttgaaga aaaatgcaac attagaacag tctctttttt
     2701 tagcacgtga agggcaaaaa gttttatctt cgcttcaaga attttcttta ggagaaaaag
     2761 cattttctgt cacaactctt aacgcacctg ctgtaggaca gcctttgatg attgcgtgtc
     2821 ctaaaaatga atctatgatg gcgatcaata gtattatcat gggggccaag gggcttttag
     2881 ggcgtgttgt tttaaaagga tcaagggcgg ctaaagtcat tacacttttt gatccaacgt
     2941 cccgtattcc tgtggatatt caaggtgttc agggcattgc agcaggaacg ggcgattcta
     3001 cacttcagtt gcttcatatc aaagatgcgc agcacacgct caaaaaagga gatgtcgtct
     3061 atacatcagg ttttggtggt atttttccca aaggacttcc ccttggtgtt atttcctcca
     3121 tccataataa aaaaatagag attttgcctt ttgagtcggg gcaggatagt tatcatgtcg
     3181 tgaatgtgct tcctcctgta tctgatgcgg atgttgagta attcaaaaaa taatagtcga
     3241 ttaaactttt tatgaatgag tcctttgagt ctctttttcg cgactatgcc gttgagctac
     3301 gaaaggtaac ggataatcct gcacgggatt tgcgtgtgct tttggggttt ttagagaaaa
     3361 aagcgcctga gttctatttt ttgcatccag agtctcttat tcctgaaaat atttgtgcgc
     3421 aactcaaaac ccttgtacag cggcggatat cggacgaacc cctttctaaa attatagggg
     3481 aaagagagtt tttcggaaga acctttaaga tcacaaagga tacgctggac ccaagaccgg
     3541 atacagaaac gcttgtagat gcagttttac attatgcaaa agatcttcca aaacccttga
     3601 ggattttaga tttaggggtg gggacggggt gtattttggt gacgctgctc ttggagatga
     3661 aaaatgcaac gggggtgggt gtggatctgt ccccagaagc attaaaggtg gcggcggata
     3721 atgccacgca ttttgggctt ttagagcgtg cttcccttag agaaggaaat tggttttctg
     3781 cgcttgatga aaaagagaag ttttctttga ttgtgtcgaa tccgccgtac attgcgtatc
     3841 acgaagtctt agaaaaaaat gtacaagact atgatcctca tttagcgtta ttttcgggtg
     3901 aaactggact cgaagcgtac gaagttattg caaagggcgc aaggcaacat ttgttagcgg
     3961 gggggctcat tgcggtggag tttggaaaag ggcaggaaaa agccgtgcgt gctctttttg
     4021 aagcagaatc ctttgaattt atagcgtatt ttaatgatct taccggggga ataaggtgcg
     4081 caatttttgc atagctagct tgcaaaaata ggattgattt ttccacagct tcattgtaac
     4141 atcctttcat ccacacacat tgaatattaa ggtttttcac ttcttatgaa gcctataatg
     4201 aagcgtccga gtaataatcg gggggcaaaa ttttctcgct cccatacgaa ccgttcccac
     4261 ccacaaggaa ggtcgagtgc tgcccagcgt aataatttgc agcaaacttt tgaaaaatat
     4321 atggcgcttg cacgggaagc atcgagcacg ggcgaccgga ttcttgcgga aaattattat
     4381 caatttgcag agcattattt acgcacattg aacgaaatga agcctattga ggttgaaatt
     4441 ccaaaagaag aagcgctaaa tagtagcgaa gataatgagg tggtttgtga cgaacctgct
     4501 cctcttgtga gtgctgcgcc gttgaaaaga gcgcaacgaa aagaggaaaa gaaagaggaa
     4561 caaccgcttc atagctaaat taaaaagctt ttggaggttt ttatgtccga ttttggtcca
     4621 tttactgaaa aagtgcagtc gattattgaa gcggcacagc gcggtgcaca aggtctttcc
     4681 catccccagt tagcttctgt acatcttttg atggagcttt taaaagatgc atttaccaaa
     4741 gatttgctca aagagagtgg tgtcgattta aactttttac agagtagtct tcaaacagag
     4801 ctcggcaagc aacctaaaat tctctccagt gatcatggtt tttcggttgc accggaggtt
     4861 ggtcaacttc ttcaattagc acaaaaaaaa gccacagagt ttaaagataa ttttataacc
     4921 cttgaggttc tattactggc tttgacagag atacagccgt ggcaagatat ctttaaaaaa
     4981 gcaggcctga accgtcaaaa actcgacgct gctattgaaa aacgtcgccg tggaaaatcg
     5041 gccacatcgc ctggtgcaga aaatcaatat gatgcgctga aaaaatatgc acgggacctc
     5101 acggaggtcg cccgccaggg aaagcttgat cctgtgattg ggcgtgatga ggaaattcgc
     5161 cgtacgattc aagtgctctc gcggcgcaca aaaaataatc ctgttttgat tggcgagccg
     5221 ggtgtgggaa agacagccat tgccgaaggt ctcgctcaac gtattattca taaagacgtc
     5281 cccgaggctc ttcagtccgc gcggttgatg gcccttgatc ttggtgcact gattgcgggc
     5341 gcaaaattcc gtggagagtt tgaggagcgc ctcaaagccg tcttggcaga ggtgtccgag
     5401 atgggggatg tgattctttt tattgatgag ttgcacacac ttgtgggggc tggaaaaggt
     5461 gatggcgcta tggatgcgtc gaatatgcta aagcctgctc ttgcgcgagg tgagctgcac
     5521 tgtattggcg ctacaacttt ggatgaatac cgtcagtata ttgaaaaaga tgcagcctta
     5581 gcgcgccgct ttcaaagtgt ttttgtggat gagccttctg tggaggattc catttctatt
     5641 ttacgggggc ttaaagaaaa atatgaactt caccacggta ttcgcattag tgattccgcg
     5701 attatcgcag cgtgtacgct gagcaatcgt tacattacgg atcgttttct gcctgacaaa
     5761 gccattgatt taatggatga agcggcgagt cgtttaaaaa tggctgttga ttctaaacct
     5821 gaagcgattg atgagctgga tcggcgtttg atgcagctta aaattgagcg cgaagcccta
     5881 aagcgtgaga cggatcaggc gacaaaagaa cgccttaaaa aactggaaca ggaaattagc
     5941 ctcctcgaat ctgaaaataa aacgctgacc gatgcgtggc ttcttgagaa acaaagcatt
     6001 caaaaaatgc acagactcaa agagcaattg gatcaatatc gctctgagct tgacatagtg
     6061 cagcgccagg gaaacttaag ccgggcggga gaattgcgtt atggtcttat ccctaaactt
     6121 gaaaaagata tcgaggattt aacggcagag gaggcgaagt ccagtgaaaa acgtaatctc
     6181 aaagaagagg taacagaaaa agatattgct tctattgtgg agcgttggac ggggatacct
     6241 gtggagcgga tgcttcaggg cgaccgggaa agattgcttg agcttgagag ccatttgcac
     6301 aagcgtgtgg tgggccaaga tgaggcggtg acggctgtgg ctaatgctat taggcggtca
     6361 cgcaccggtc ttcaagatcc gagtcgtccg cttggatcct ttcttttttt gggcccgacg
     6421 ggtgtgggaa agacagagct ttcacgtgca cttgcggagt ttttatttga tgatcccgcc
     6481 cacatgctcc gtcttgatat gtcagaatat atggaaaaac attcggtatc gcggttgatt
     6541 ggggctcctc caggctatat cggatatgag gaggggggcg ttttgacgga aagtgttcgg
     6601 cgtcgtccct atcaagtcat tctttttgat gaaattgaaa aggcccatag tgatattttt
     6661 aatattctcc ttcaggtcct tgatgccggg cgccttaccg atagccaggg gcataatgtt
     6721 gattttaaaa acacactgat tattttgaca tcaaatttgg gcgctgagta ttttagtgca
     6781 agtgatgcca tcacacccca tgtgcgcgac caagtgatga gcgcggtgcg tcaccatttt
     6841 cgtcctgaat ttttgaatcg gttggatgag atcttgctgt ttagtcgctt aagccgtgaa
     6901 aatttgcgtg gcattgtgat gattcagctc aaagagcttc aagaccgcct taatgcagcg
     6961 catcaggtga cactcgtcgt caaggaatcg gctcttgact atctgggcga tgttggtttt
     7021 gatcctcttt atggcgcgcg accactcaaa cggttgattc aacgggccct tcaagacaaa
     7081 cttgcaacag cccttttgtc gggcgagatt agccctgggc aaacagtaac agttgacgct
     7141 gtgaatgacg cgcttgtttt tatttaatgt cgctaaagga gaaaatagat atgaaaaata
     7201 taacgtttga gcaagccctt cttgagcttg agtcccttgt ccgcacctta gaagaggggc
     7261 atatgccgct cgcggattct gtcaaagcct atgagcgagg gattagtctt aaaaaatttt
     7321 gcgaggaaca gctcaatgct gcccgtctta aaattgaaga ggttgtggcc gctcaacata
     7381 aaattgaggt gaaacccttt gaggtggagt cgtagagaat ttttttagag tcatttagta
     7441 aatgtatata ttatatgcgt ttttactaaa tgactctaga aatctagccc tttattcaat
     7501 ttttcaggct cttctcttat ccttcaatag catcctcgca ccgtccacag atagcattct
     7561 ccattgttac ttcttctaaa atgcgccaac agcgctcgca ctttccgccc cctgcttttc
     7621 gcacaacaac actcaggtca ggtacatcac tcagagtaaa ggcatgcgca ggcgaggtgt
     7681 ctatgagaag agttgccgtt gatgtaattg caaattctgc aagatcaata ccttcaaaaa
     7741 gagctgcaat cgcaggcgtt gtgtagatta agagcgccgc ttgtaaactc gatccaatcg
     7801 tttttgccgc acgctcaacc tcaagggcgc ccgtcattac acggcgataa tggcgcagtt
     7861 tttcccattt tgcggcaagc gtttgattaa aatagacctc tggaagcgcc acaaacgttt
     7921 ctaaatgaat acttgttttg cgatcattgt gtgcatggga aagatacgcc tcttcggcgg
     7981 taaagctcaa aacaggagca agccaacgtg tgaggcaatg aaaaagtgtg tccatcactg
     8041 tgcgcacagc acgccttttc agacttgtcg tgtcgtcgca ataaagacta tccttgcgca
     8101 gatcaaagta aaatgccgag agatcgcttg tgcaaaaatg gtgcagctct gtataaaaag
     8161 cactaaaatt aaatgttttt gccgtttcat tcattgtgcg ttgaagctca gataaacggt
     8221 gaagaatcga gcgctcaagc tctggcatgg cgtcataagg aaggtgttcg ctttcttcaa
     8281 aaccacttag ggcgccaagg agatagcgta atgtgttgcg aaaacggcgg tacatatcct
     8341 ccatttgttt gagaatatct ttgccaatgc gcacatcgtc actatagtcc acagaagcaa
     8401 cccacagacg taagatatcc gcaccgtaag ttttaatgac atcctcgggg gcaacgacat
     8461 tgccttggga tttggacatt ttatagccct tttcatccaa aacaaaacca tgagtcacga
     8521 tcgtgtcata gggcgcgcgg cctcgtgtgc caacgccatg ccaaagggag gattgaaacc
     8581 atccacggtg ttggtcagag ccttcaaaat aaacatctgc aggccactta agttcttcgc
     8641 gcgcttcaag aacaaaagca ttggtgcacc cagaatcaaa ccacacatca atcacatcaa
     8701 aaatttgctc atagtcatcg gcatgataat catcgccaag aaattcttga gccggtgttg
     8761 taaaccaaac atcggcacca tgggcattaa ttttttccac aatccgcgca tgtacagcct
     8821 catcacgcag cagttcgttg gtttttttat gcacaaaaag tgttaagggc gttccccagg
     8881 cgcgttgccg tgaaatgcac caatccgggc gactttcgac catgcctgcg atgcgatggc
     8941 ggccttgctc aggaataaaa tgcgttgctg caacggcatc catcgccttt gagcgcagtt
     9001 gtttcacatc aataaaccac tgatgcgtgg cacggtaaat aagcggtgct ttggatcgcc
     9061 aggagtgtgg ataactatgg gtgagtttgc tttgatgaat cagcgcaccc gtttctttta
     9121 acgcgtcaat aataggtaca ttcgccttaa agacgtgaat cccttgaaaa tgaggaacct
     9181 catgggtata aaaaccatca gggccaacgg tattgggaac aggaagatta tgttttttgc
     9241 caagaataaa atcctccatg ccgtggcttg gggctgtgtg aacaaggcct gtccccgctt
     9301 ctgttgtgac atgatcgcca aagaaaaaag gaacatcaaa ggtgtatcca aggcgataaa
     9361 gagggtggcg tgccaccgta tttttaagtt tctcgccggg gatttttgct aaaattttgg
     9421 tctcagcatc ctctagcccc atcgcgctga gcacagcttc taaaagagct tcggcgataa
     9481 ttaatttttt ttcattgtgt tcgatgacga cataatcaaa gtgaccatag gcaatcgcac
     9541 ggttgccggg aagggaccac ggcgttgttg tccaaattac ggcatgggca ccgtctaaga
     9601 taggcacatc tgtttgcgta agaggaaaag cgacataaat cgcatcgctt gtgtgatctt
     9661 tatattcgac ttctgcctct gcgagcgctg ttttttcaat cacggaccac atcacagggc
     9721 gcatcccacg aaagagcgtg ccgttcatca gaaattttcc aagttccgcg acaatggcac
     9781 cttctgaggc aaaatccatc gtcgaatacg gattgtccca atcacctata atcccaaggc
     9841 gtttaaattc ttctttttga atcgcaaccc attccttggc aaacgtgcga cactcttggc
     9901 gaaaggtaat cggatcaatg tcgtctttac gaaggccctt ggcctgatat tgtttttcaa
     9961 tttgccattc gatgggcaaa ccatggcaat cccagccagg cacaagaggc gcgtcaaatc
    10021 cctgcatttg ttttatttta ttcgtaatgt ccttaaggat ttctgataat gcgtgtccaa
    10081 tatgaatatg accattagca taaggagggc caaagtgaag aacaaatttt tcctttcctt
    10141 tgcttttttt acgcaaggtt tgatagagat cgagttgatt ccaatgcgcc agatagttag
    10201 gctctcgctc tgcaagctgc gctttcattg gaaaatcagt ttgaggaaga aaaacggtct
    10261 ttttataatc aacggtggac attatttttg tcattttaca acagcttttc ttctcttata
    10321 cacgaatgac ggatcaagag gatatgagat tttttaagat acgcttactt agttaaaaac
    10381 gtcactgata tccacaagat cataggcgat tttttgatca ttcagcggca cattattggg
    10441 gttgagggaa aaacatttat actcaatttc agcatatttt cctccaaacg gagagtcata
    10501 ttcgtagttt tttttaatct cccgtattgt cgaaagatca acaataccga cactgatatt
    10561 tccctctaag tcaattttca tcgcaagtcc attggttttc tcaaaataat tgctattttt
    10621 gaaaatatct tttttatcgc ccgtaaatgt ttgaataatc caaggagaag gggactcacc
    10681 aatatctttt tcttcgcgaa gggtgtttgg gcaataaatg cattggattg tttgcttttc
    10741 ctgctcaata agttttaaaa tctccaaaag cctttgcgac ctataaagag tacccattgt
    10801 gtggattaca tcgctgggct ttgcgtcgcc ttg
//
LOCUS       DAIAHG010000019        10626 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_19_length_10626_cov_3.229880, whole genome shotgun sequence.
ACCESSION   DAIAHG010000019 DAIAHG010000000
VERSION     DAIAHG010000019.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19_length_10626_cov_3.229880"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2901 a         2292 c         2317 g         3116 t
ORIGIN      
        1 gagaatcgga tcaggtgcgc tcgcaccgac gtaacgcatt gataacgtgt ccttggttat
       61 gcaagactaa taatcgacaa tctataaaag cagctaaaat ggatattcat attcgcaaag
      121 ctactcttaa tgatcttgtg tctatcgtgc aacttttata tgaggacgag attagtcaag
      181 agcgtgaggc gctgagcaca ccgcttgatg cgtgctatgt tgaggccttt catgcgattg
      241 atcgtgatcc tatcttatga ttgcagaaca taaaggggaa tgcgtgggga cctgtcattt
      301 gacctgcttg ctgtctttaa cttttaaagg gacaacgcgc ctacagattg aggctgttca
      361 tgtgaaaaaa acgcatcaag gtcagaaaat tggcgagcgt atgatgcatg aagctttcgc
      421 ctttgcacag tctcaaaagg cacggattgt tcaactcaca acgaataaaa aacgtgtgcg
      481 tgcgcaaaaa ttttataaac gacttggctt tgaagcaagc catgagggca tgaagaaaat
      541 catcaaataa tattttatat tattctttta gtgcttactc cctgtgccct cttttattgt
      601 aaatatttcg tcggatcaag gggggttata ttttgccgta tttcaaagtg cacttggggt
      661 tctgaaacac ccccacttgt acccactgtt ccgacttttt gtcctgcggc gactgtatca
      721 cctactttga cagagcagtc ctggagatgg ccgtagacac taattttccc acctgcatgt
      781 ttaatgagca caagatttcc aaaacctcgg gcgtgattgc ctacatgggc gacaacgcct
      841 tggtcagcag cccctacagc cgttcctgca gccgcggcaa tattaatacc agtattgtgt
      901 tgtcctttgg ggtccacgcc aaaagactta accactcgac cttgtacggg ccatttcatc
      961 gtgccagtgt tttggggaat ctttggagcc gtttctcttg gtagagggcc ggtccccacc
     1021 atatcgtttg tttcagccat aggcatagcc gtattgttag acggagcatt gacaggtgct
     1081 gtgggcgatc ctatgacgcc acttcccatg gttggaaggc ccatccctgc acttgctgca
     1141 cctgtgcctc ctgcaagtgg gggcacagcc ccaccttgtt tttcttcttc agggggaagt
     1201 tcttccaccg tgacttcgcc tgtgtcggca acactcgtga caggtttcac agaaggcttt
     1261 tgcaccgcac ttttatgaga tcctgcgagc acaataagtt tttgcccgat aaaaatttta
     1321 taagggggtt taagacggtt aacctcaatt aaactttctt tggacatact atatttggat
     1381 gcgatcatcg ataaagtttc accacgctcc acacgatgaa aaggtgtagg cgccttatcg
     1441 accactactt ctgcaggggg ctcctcgcgc gcacaactcg ttaaaagaag tgataaagcc
     1501 aaagatgcaa aataaagtga agggtgtgta gacctgatca tagcaaataa attaaaaaga
     1561 agccctcgat aatgactcta gtattgccat acttagagcc atcgtcaaac agaattcttg
     1621 ccttagcagt gaattttgtg cgctttttta tttttttacg cctacgtaaa tatcgagcac
     1681 tgtgaggaag gggtccgccg cacgttcgtc gtaaatctca aagtctgctt cataaagacg
     1741 ttctgcgttt tcttcctgct gtgttagcca gatatgctgc caggcattaa tacagacctg
     1801 tggcatgggc cctggaaagg ttgtgtattt aagatagtgt tgggcgggaa tcgtgatggc
     1861 tgtcatcccc tctggcaatt ccttgagctc tgtgacttcc tcgccaataa aatacgtgta
     1921 atcaccgcga taatcgcttg catagtcggt atagacagag tatgtcgttc cgggcttttt
     1981 ccggttttgt attttctcgg catagttttt atgcaaataa tcctgcgccg tgagaccaat
     2041 tttagcagtt aatggattca tctcgttcat aagattggtg cgtgctgtaa tccctacaag
     2101 atgaatctcg ggcagtgtag tttcggtttg ttgcattact gaatatcctt ttaaagctgt
     2161 ccataggcta tcttaactcg caatggcgag atagttgtca taattttctg ttaagttccc
     2221 aaattttgtc acagccccat caaaataaag gcgcacagtg ccaatgggac catgacgttg
     2281 tttggccaag ataacctcgg ctttgttata cattttttcc atgcgtgcct gccactctgc
     2341 gtgtttatct gtgccaggcg ttggctcctt gcgtccctca taatattctt ctcgaaacac
     2401 aaacatcaca acgtcggcgt cttgctcgat ggagccggat tcgcgcaaat cagagagttg
     2461 agggcgttta tcatcacgtt gctccacggc acgagagagc tgtgagagtg caaggacagg
     2521 tacattaagt tccttggcta aggctttgag gtgacgcgta atttgagaaa tttcttggac
     2581 gcggttatca gatttgtgtt cgcctccatc caaaagctgc agataatcaa caacaataag
     2641 gccaagacca tgttgacgtt gaaggcgacg tgcacggtta cgcagatgcg tcacagaaag
     2701 agcaggggta tcatcaataa aaagaggcgc ctcataaata cgacgactgg cctcgataaa
     2761 ttttggaaaa tcttctgtgc ggatatcccc acggcggatt ttatccgatg aaatgccgca
     2821 ctcactggca agaagacgcg tcgcaagctg ttctgaggac atctcaaggg aaaaaaaagc
     2881 aacaggcgct cctgtggttt cgccgcgcat tttagacata gcggcattaa acgcaatatt
     2941 cgttgccagt gctgtttttc ccatggaagg gcgccctgct aaaataagta aatccgaggg
     3001 gtgcaatcct ccaagccatt tgtctagatc ggtcaaacct gttgtgactc ccacaatatg
     3061 actatcccgt ttaaacgcaa tttctgccat gcccacagct ttgataatcg cttctctaaa
     3121 actaatgagg ccagagtttc cttcaccttc catcgccagt gtataaagag cacgctctgc
     3181 ctgttcgatt tgtcctttag catcatgatc gagtgtgggg gtgcgggctt tatcaataat
     3241 atcttcgccg atatccatca attgacgctt aagataaagt tcataaatta attgaccata
     3301 gtctttgacg ctgacaatac tcgtaaaacg cgtgcacaaa tccacaagat attgagaacc
     3361 atgggtagcc tcgaactgtg ggtgatcttt aaaaaaatct ttgagcgtaa tggggtctgc
     3421 cacttgacca ttttgatgca ggcgttgaat agcagaaaaa atgagctcgt gacgtttgtc
     3481 ggcaaagtgc acagatttta aaacatcagg aagtcgttca atatgatgat tattgagcag
     3541 aagagcgcca agaagggctt gctctgcatc aatattataa caagtatggg ggagagaaga
     3601 gggcagtgtc gtcatggtga agcaatcgaa aggaagatca tctcactcta gcacatttta
     3661 gcgtggatgc tcttcttttg tggattaaat acgcgtggga taaaaaaagg cacttcttaa
     3721 aaaaattaaa aagcgcattt tctgtgaaat aatttagcta ttttaaaaag cttacccctt
     3781 tgcagcggtc tcgctctttg aggatgcccc cgtcgatcca gattttttcg tatatgtttt
     3841 cagcggttga ttattattat tgacagaatc tttgttaaga acaacctctt caactttttc
     3901 atagcttggc agctcgaaca ttgtatccaa aagaattgtt tccataatcg cgcgcagtcc
     3961 ccgtgcacct gtttttcggc caatagcttt ttgtgcaatg gctgtaagag catcatccgt
     4021 gaaattaagt ttgacattct ccatttcaaa tagacgcttg tattgcttga caagtgcatt
     4081 tttgggtttt gtgagaatat cgacaagagc ggcttcgtca aggtccgaaa gcgttgcaat
     4141 aatcggcaga cgcccaataa actcagggat aagaccaaat ttgagcaaat cttcgggctc
     4201 cacatcgcgc aaaatttcgc ccgtctgacg gtcatcgggg gattttacat cggccccaaa
     4261 accaatgctt gccccctttc cacgggcaga aataattttc tcaagccccg agaaagcacc
     4321 cccacagata aacagaatat tggttgtgtc tacctgtaaa aattcttgct gaggatgttt
     4381 gcgtccacct tgagggggga cagaagcaac ggttccttcc ataattttca aaagcgcttg
     4441 ctgcacacct tcacccgaaa catcacgtgt aatggaagga ttgtctgatt ttcgggaaat
     4501 tttatccacc tcatcgatat agacaatgcc gcgttgtgcg cgctcgacgt tataatctgc
     4561 ggcttggaga agctttaaaa taatattttc tacgtcctca ccgacataac ccgcttcagt
     4621 gagtgttgtc gcatccgcca tggtgaaggg aacatcaatg attttagcca atgtttgcgc
     4681 gagcaaagtc ttaccgcttc ccgttgggcc tacaaggaga atattcgatt ttgcaatttc
     4741 cacatcgccc gatttcccac catgggcaag gcgtttatag tgattgtgca ccgcaacaga
     4801 aaggactttt ttggcatggc cttgaccaat aacatagtca tcaagaactt ggcatatttc
     4861 caaaggtgta ggtacaccct cgctaacgcg cccgcccgtt cggctctctt ctcggataat
     4921 atctgtgcaa agttcaacgc attcgtcaca aataaaaacg gtcgggccag cgataagttt
     4981 gcgtacttcg tgctggcttt tcccacaaaa agaacaatag agtgtgtttt tattttctgt
     5041 atttgaattc ttgctcatag tatctccggg caaaaaggcg gctcttggtc cgcccctttg
     5101 cgctttccaa gaatttctac ccctagtatg agtgagaaat tctcaataaa aagttaattt
     5161 attcgcttaa tgatgaagaa atttcaagaa ctttggggcg ctcgacaatc acatgatcaa
     5221 taaggccaaa atccttggct tcatcgggtg acataaaacg atcacgctct aaaacttctt
     5281 caatttttgc tattttttgt cctgtttttt cagcatagac ttggtacaaa agagcacggc
     5341 tcttgagaat ttctttggcg tgcaactcaa tatccgttgc ttgtccacca aggccactca
     5401 catgaggttg gtgcaccata atacgtgaat gcttgagggc ataacgcttg ccttttgtcc
     5461 cagccgtcaa aagaaaagac cccatagaac aggcttgtcc catacaaatc gttgagattt
     5521 ctggccgaat ataattcatc gtgtcaaaaa tggacaaccc tgcagaaaca gatcctccag
     5581 gggaattaat atacatcgca atatccttat caggattttc tgcttctaag aacaaaagct
     5641 gtgcacacac aacacttgcc atctcatcat gaacagggcc tgtgagaaaa ataatgcgct
     5701 ctttgaggag gcgtgaataa atgtcaaaag aacgttcccc gcgccctgtt tgctcaatta
     5761 ccataggaat gagcgtgctt tgaggtgccg ctccgtaacc tgacagacct tgatgtgcaa
     5821 tggggccttg gaagggcaaa gaattataaa atgacatcgt aacgcgtctc cttagtcgtt
     5881 agaaaaaaca atagaggtac tcctcaatag agtaccatca tttatcctac gctgcatcct
     5941 cattatcaaa aaatgtaggc acaacgcctt tgactttttt cttaaggcct ggcacatcga
     6001 ctttgacctc agttgttttg atttttgtgg caataaagtc aacaacctta tcctcaagga
     6061 taggcgcaac aagacgatca atcatgtgag ggtgactgcg ataatactcc ataaccttgc
     6121 gctcttgtcc tgtgtaacgc atggcttcac ggtaaatagc attacgaagc tcatcttggc
     6181 tgagtgtgat tttttcctct ttcgcaacgt gagaaataat caaaccaagg cgtacgcgtc
     6241 gctcggcaat tttttgatat tctgttttaa gttcatcttc aggcttatcc tcatcttcga
     6301 ttttctcacc gcgtgctttt gcttgcgcaa gttcattttg aagatgttgc caaatattat
     6361 taaactctga tgtgaccatg ctttcgggaa gtgggaaatc ataagctttc tcaagggcat
     6421 ccaaaagatg gcgtttgaga taaagacgtg cgatagagct ataattcgac tgaagatttt
     6481 ctttgagatt tttcttcaaa tcctccagtg tttcttgctt aaattctttt gcaaactcat
     6541 cgtcaatttt tagtttttta ggcgcagata cttttttaac aaccagcgtg acctctacat
     6601 ttttgccagc caattgagca tcgccaaaat tatcagccac tgtggagcta aatgtctttt
     6661 tctcaccctc tttcaaacct gttgctgcat catctacacc ggaaagcata aaagccttgt
     6721 caccgatatc taatgtgacc gaagcatttt gatagccgct cacctttttg ccattgagtg
     6781 aaagagaaag ctcacatgca atacggtcgc ctttttcagc agcccgacct tctttatgtt
     6841 caaaagattt gtggttttta tgaagatctt ttacagcttt ttcaacttct tcgtcagaca
     6901 tatcaacgac aagttgatca agctttatct tagagaaatc agtattttta atctcaggca
     6961 taacttcaaa cgtaaggcta aaagaaagat ccttgccttc ctcaaaatca ccaagatcca
     7021 ctttgggctc attaatatat tgaagattgt gctctttggc cgttgctttg atagaattat
     7081 caataaaagt acgcaaagcg tgattaagtg catctccccc gagtctttgc tctaaaacag
     7141 gcatgggcgc tttgccagga cggaatccgt caatttttat tttacctgca cgggatttga
     7201 tataagcaat tttctgctcc gcgatgtctt gtgcagaaac aataacttta aattcgcgtg
     7261 ttaagccttt tgatgataaa atctctgttt gcatatgttt gatctctaac atttatagtt
     7321 ggtacgggtg aagggacttg aacccccacg actctcgtca caagaaccta aatcttgcgt
     7381 gtctaccagt tccaccacac ccgcatgata ccttgcttct atatagacat actcagcact
     7441 cttttgtcta gcattagtat gtatggattg gaaggagaat tttaagaaag agactgaaaa
     7501 atagtgctgg tggaaaattt ggaaaaaaag agggctcaca acgcaactat atgagccctc
     7561 taagtctatt ttttagattt agatattaag gcgttttggc gtgtttttga ttttatgctc
     7621 gataaggttg atcaagtgct caagtttttt ctcaggaaac tcttcaaggt tgaaattttc
     7681 cgcttcagct atttcctgtt gatgggcctt gatttgtatt tctttgattt tgagggcggc
     7741 attaacattc tcctttccaa gagccatctc atataatgtg tcgaggtctt ctaaataatc
     7801 cttccatggt gttgggttgg ttaccatgcg atcctcctgt tttttcaact ccatctcatg
     7861 tgcaaaaatt gagaaggaat tttcaaaata gcataaaaaa acaatgggaa acgctatcta
     7921 aaaagtccct tgctccaggg ttttgttttt tataaacctt gcaacaaagg tgaaaaatga
     7981 ttagaattct tctgttttat gaattatctc agcctatatt gaagattctt tctgtaatat
     8041 ttagtgctga tgttccctgt ttatatgaga gacaatgcgc gttttatttt tttcatcgct
     8101 tttcctgagt gcaacacttt ttgtggcatc aaaagcagat tctgcttctc catcgtctaa
     8161 atccacacca tctgcatcca caaaaaatgt cggtatcgtt gccaaagtgg ataacaaact
     8221 gatcacggaa aaggatttag agtctcgtgt aaaacttatt cttgtcacga atcatatccc
     8281 ggttacggct gaaaacatca aaaatctgcg cgagcaagtt cttaaaaata tgatcgagga
     8341 atatgtccaa gtggctgttg cggcgaaata taaagtactc gcgaaagagg gagatgtggc
     8401 ggcttccttg gaccaaatgg ccaaagacgc gcatacaacg gccaaagacc tagaggcgat
     8461 gctgcttaag aatggcgttg cgattaatta tttaaaagat cgtatcaggg cgcaaatttc
     8521 ttggattaat tttgtgagag gcggctatgc tcacaccttg cacaccagcg ataaagatat
     8581 cgataagcag gttgccaaag aaaaagaaga tgaagctaaa gatcagttcg aactttatga
     8641 gatctttttg cgtgcggatg atgcttctca attgtcagca gtggaggctc aggctcaaaa
     8701 gcttattacg caacttaaaa atggtgtcag ttttagaaat cttgcgcagc aattttcgaa
     8761 tagccccaca gcttcaaaag gaggctatgt ggggtgggtg actgcgaatc acgagggggc
     8821 gtctgcttac caaaatctgg agccggggca agcctctctt cccgtcaaaa caaatcatgg
     8881 ctattatatt tacttcttga acgacaagaa aaaaggcggc caacctgcca tgggggatac
     8941 gcttatctct tacaagcaga ttacaattcc attgacgcct gggtttaagc ccgaagaaga
     9001 tccttatctg aatgggcact tagcggaggt gatgggatca aaaagctctg cggcttttga
     9061 gaaggcagcg cgggagcgtg atttaaaaat agaaacggta gcggatcgta aattagcgca
     9121 tttctcaccg gagtttcaag ggtttgtgaa atcattggga atgaatacgc cttcaatgcc
     9181 ctttcccaca gagcacggta tttctgtgat tatggttgtg aataaaaggg taggagaacc
     9241 gcctaagcct ctcacgcggg atgaaataaa ggaaaaaatt gaaagtgaaa agctttcaaa
     9301 ggttgccgcg cagtcgctta cgcagcttat ttccaaggct catattgttc tgactaatcc
     9361 tgaggagttt ccaagtcttc cttacggcaa aaagaatgcg ctgcaaaaaa catcaacact
     9421 ccctgtagag gatgctggct ctgtcaaagc ttagtgatct tccgcccctt aaggatcttt
     9481 tgattgagca tgatctgtgg gcgaaaaaga gtcttgggca aaatttcttg ctcgatccca
     9541 acattacgga aacgattgct aaaaaagcac tgattaacga tcgtgttgtg ttagagattg
     9601 gcccggggcc tggtggactg acgcgagggc ttttaaacca tggtgcgaaa caggtttttg
     9661 cggttgaaaa ggatgtccgc tgcattcatc tccttcaaag tcttgtaagc gcaagtgagg
     9721 ggcgtttgca ccttatcgaa aaagatgctc ttaaattcaa attaagtgat tttgtgacag
     9781 agtattccct tgatgcgccg tttcatattg tggcaaatct tccctacaac attggcacac
     9841 agctcttgct gaattggttc aaagatcttg attggatcgg ttccatgacg cttatgtttc
     9901 aaaaagaagt cgctgagcgt atggtggcta cgcctggaac ggatgcttac ggtcgcctca
     9961 gcattctttg ccaatactta tgtatgtgta agattattat gcatctgccg ccgtcggctt
    10021 ttacgccagc gcccaaggtg cattcggctg tgattcagct tgtgccaaaa aaactaagtg
    10081 atgaagaaaa aaaacttctt cccttgctcg agagagtaac ggcccttgct tttggccagc
    10141 gccgtaagat gcttaaatca agcttgaaat cccttttaaa ggaaaatgag atcgaagccc
    10201 ttggtatttc tccgacaatg cggccggaag agcttgccct ggatagtttc ttaaaaatct
    10261 gcaaaaaatt aatttaattt tataattatt tcttttttta aaaacatttt attttttaaa
    10321 aacattttat tttttagtgt caattattta ttattaattt tttaatttat tttgcagcga
    10381 gaggagcttg aggcaacctt ggccgaggag cgggcctcgc tcgagagaga gcgctcccag
    10441 tgggctgggg agcgttcgca gtgggccgga gagcgggcct cactcaccac tgagataaaa
    10501 aaaattattt ttaaattaca tcttgtttca attttacttg tcaatttatt aattatcttc
    10561 cacttgattt gatttttagc ttgataagag gatgagggag caggcccaat tgatagagga
    10621 gctcga
//
LOCUS       DAIAHG010000020        10117 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_20_length_10117_cov_3.385956, whole genome shotgun sequence.
ACCESSION   DAIAHG010000020 DAIAHG010000000
VERSION     DAIAHG010000020.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10117)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20_length_10117_cov_3.385956"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2945 a         2006 c         2081 g         3085 t
ORIGIN      
        1 tcgattttcg atcttagttt gccaaaatca aaaataggaa aagacattgt gggttgtgct
       61 gtgaaaaaag aacttttaga tttgaaaagt tgattcgtat tcattccctg ccagccaaga
      121 gttcctgtca gtaaaaatga ggggaaaaga ttagcaaaag aaacgcccac cgatgctgtt
      181 gcggcggcga ggtctctctc tgctttgcga atatcggggc gctcaaaaac aattttagag
      241 ggaagacagg aaagataaac tgtgtcagct gagggaatat caccaatatt ttcgagccgt
      301 ttgtaaaggg cccagggttc tttgccaaga agaatgccta gctgatgaat acattgcttt
      361 acattcgtat cggctgttgt gacaaggcct tgagcttgga gaagagatgt atcaacgctg
      421 ctgacagcaa tgtcggtggc aaggcctgcc tgataaaggt ctttttgtgc acgcagatac
      481 tcttcccaca aatgaacgat atctttgagg gttttatact ggtgttgata aaaacgaaga
      541 ttaatataat tttgagcaac ttcagccatc atagtaatga gaacagagcg actgtcttca
      601 atagaggagg ccaaagacgc ttcagcagct tcattggcgc gctgaatgcc gccaaaaata
      661 tcgagctccc atgtcgcatc aaatcctgtg gagaaaagag aggcattggt tcccggagaa
      721 ccagaaagtg agccgctttt gcttgatttg taacgagtgt ccgaggcact ccctgtgagt
      781 gcagggaaaa aagaagcttt gcttgaggcg acatttgcgc gtgcttggcg aataacgctc
      841 aaagcttttt caatatcaag attgcttatt tgcgcttctc gaatcaattg tgtgagcaaa
      901 ggatctttaa atgtttccca ccaacaaaga tctttgttgg catctggact tggtgttgtg
      961 ggagctttat tccaccaaaa aaagggcaaa gatacctcgg gtgtttgata gttaggcccg
     1021 acagcacagc ttgcaagcgt aaaaaagatg agagaaaaga aaaatgatcg gaaaaaacta
     1081 ttcatgattg gctctttttt caggtgcttt tgggggcgca tatttttgat cacgctcttt
     1141 aggaacatca atataaacat caacctgttg tcctatgaaa agagggagat ctttggggtc
     1201 aaaacgataa atgacttgaa gaacacgggt atccacgcgc tcgctggatg tgcctgtgag
     1261 tgacgttttg gggacaacat agggctcttc gcgcacaaaa gtgagataaa attttatagc
     1321 gctattgccc cgtagatagg cgatcgcttt tgcttccctt acaaagcgcc atgcctgatt
     1381 ttcatcaatg tcaatgcgca catgcatttt ttgaaggccc cctaaaagca taagaggtgt
     1441 attgacggga gatgcgatat aatcgcctgt aagaggagat tggcttgcaa gttctcccgg
     1501 gtgaatattc acttgaagag cctgacaatc catgggcgct ttgacggtca gcaaactaag
     1561 atttgttata gatgctttta cgttggcttc tgccacatta aaattttcaa tcgaggcctc
     1621 ataggccgct ttgtctgtga gatacgcatt tttacgcgca agaaaatcat cttggctaat
     1681 ggcacgctta tctttgacac gattcgccag atcatactta tccttggaag ctttcatctg
     1741 agcttcagct tgtttcatca acgcttttgc ttgggacatt tgtgcttttt taagttttaa
     1801 gtcatcttct gcttgacgtg tatccaatgt aaaaagaggc atacctgcgt ttacttgatc
     1861 accaactttc acatgcactt gctgcacaat gccttgaata tttgtactaa gctgaatgtt
     1921 atctgttgca ctttcaataa tgcctgtggc tgcaataaaa ttttgataaa tggaggatgg
     1981 aggcggtgat gctggaagcg cgacaggaag gtgccagctg ctacgaatga caagaaaaat
     2041 agctcccaag atacccacaa gagcgaacac aatgatactt gtgttaaaat caacctttgg
     2101 tttcccattt gaaaaaaaga gatctttaaa accttcatac atcatttgta tcgaatgata
     2161 aaattttctt ttaagatgat taatgtccat gaatatcctt tctataaggt ccaaccctta
     2221 aaataacacc atcttccatt tcagcaatgc ggtccccaaa ctcaaaaaca cggctgtcgt
     2281 gtgtcacgac aatcatggcg ttatctgtac cgcctactaa actttttaaa agtgtcataa
     2341 ccctgtgacc atttgttcca tcaagcgcac ttgtgggttc atcacacacc acaagtttgg
     2401 gacgatgaac aatagcgcgt gcaatcgcaa cacgctgttg ttgtccccct gagagttgaa
     2461 ggggatacga acttttttta tcagcaaggc ctacatgttc taaaacattt cctgcctcta
     2521 ttgttgcatg ttttcggtcc atgccattaa taatgagggg gatcgaaaca ttttcagcgg
     2581 ctgtgagtgt gggaataaga ttaaattgtt gaaacacaaa accaatttgc tttgcgcgaa
     2641 aaagagcaag ctcctcttct ttcatgtgcg tgacatcttg accaaaaaca gcgcagaacc
     2701 ctttgtcatt ttttaaaata cctgaaatga tggaaataag tgttgttttt ccacagccag
     2761 aagggcctgc aagcaaaaga agctctcctt ttctgacatc taaatctgtg ccacgaagcg
     2821 ccatcacctc tgaatcgcct gtggaaaaag ttttcgtaat tccctcacac ttcacagcaa
     2881 gctcagaaga tttctcagtt tttatttttt tatcctttaa agacaatggc cgtctccaat
     2941 ttaaaaactt tgcgaatgct caaaagggct gcaagtgtac agataaaggc aacgcctaaa
     3001 atactaagga tcataagttg ccaagaaaat ttaaaggcaa gaatcgagtt gcgcgaaaga
     3061 aaagcaaaaa tagacgttcc aagagccccc agcccgtatc cgataacacc aacgatcatg
     3121 gcttgtaaaa taatcatcaa aaggagggtg cttgattgaa tacccatggc tttcagaaca
     3181 ccgaaatagc gcaaattttc cagtgtaaaa ctgtaaaaag tttggcctgc aatggcggca
     3241 ccgacaagaa atccgagtaa aacggatgtg ccaaaattaa tggggatccc tgtgtatttc
     3301 atgtaataat caagtgtttt atcttcaaaa tctttctttg tgtaggcggc tagtttcgtt
     3361 ttttcaacaa tacgattcgc caggatatga gggtcttcat tttttttggc tttgaccaga
     3421 ataaaagaca gaacattacg ttggggaggg gcaaaggctg tagctcttga ataggttgta
     3481 tagagaatcg gcatcgattg aaaggtgcga gaggtttcgg caatacccac gacaacagaa
     3541 taattatcgt taagttctaa aacatcaccc acttttaagg ggattttcga gccatcggga
     3601 agtgttcttg caagtttgtc aagggcccct tcgaggttag caatgacact attatcctga
     3661 taaagatctt ctaattttcc ttccaacatc ttcccaggcc caccaataag cgtcgcatca
     3721 tcaaggccga tgacattgca attttgaatg tgtccattcc ccaggcgcac ttgaatttgt
     3781 cctttgaaaa ggggcttggc ccattccaca ccatcaatac ttccaacgcg ctcaagttct
     3841 gtgcttgtaa ggggttttgt atcatcaatg tactgtacat cggggtccat cacccaaata
     3901 tccggcaagg aaatatcgct gataaagcta tacgttcttt ccataagacc caaaaaaatt
     3961 ccgggttgtt gtgtcataat gagcgccgca aaggaaatgc caaggataat accgatatat
     4021 tttcccctgt ctccgatcag cattttaatc gcaagataga acataaaatg atcaggaatt
     4081 ttttttccta aaaatcagat tagagaaaaa tattaaattt tttattaatt ttttaaaaaa
     4141 ataggcttga aaaacgacaa tttaatggtg ccatagattt tctcaaataa gagctggaga
     4201 gaagagatcg aaagcttttc gcttttgtct gtttcaatca caatcagtgt ttcagggaga
     4261 taattttttt ttaatttttc aagggcataa gcataaaggg gcatctcatg cgtatattca
     4321 aagtaagggg gatcaagaaa aataaaatga gcaggccttg tggccctatc aaaattactc
     4381 agaactaggg actgatgctc taattttaat ttacgaatat tgctacgaag aattgcctgg
     4441 acggacggtg ccttttcgat aaaagtacat aaagcagccc ctcttgaaag aaattccagg
     4501 cccatggccc cacttcctgc aaagccatca ataacgtgaa tagcgttggt gtgaaaatcg
     4561 aggctgaaga ggcagttaaa aagggcttca cgcgcacgat ttgatgtggg gcgggtaata
     4621 ttttcatcgg ggagaaggag ttttgcgccc ttaaagcgcc ctccgattat tttcagcata
     4681 attttttatt tttttgtgaa cggaaattaa ttctagaatc tttgagagag gcgttctagg
     4741 attttctttg gaaaactttt agtgtggata aaaaaacaca ccacgcctaa gcaatgatgt
     4801 gttttttaag gtagtaatac ctatttattt tgtgataagt gcctcaggct ttggggcgtc
     4861 atcgcttcca cgaacgcttg gtgccaaatt gcccacacgg tttttattaa tgtcaggacc
     4921 tgcagcctca gcaatatgtt gctcgtcttc acgcgccact tctgcttcga atttgcggtt
     4981 ttgctctgac ttcataatac ctacgagaag tgaccatcct gtaagaccac gcgctttgag
     5041 gtcgcttgtg tcaaagaatg cacgaacgcg ttgaatctca taaggcttgt tcaaatccac
     5101 tttagccatt gcatgaccat ggctgaaaac ttcgttggaa ggaatatctg ccgaggagaa
     5161 gaacaagggg acttcaacaa ttggaaggcc gttggatcca ccagagtgct ctgccttgag
     5221 atcaaaaaca acctcaatca ttgcaggatt gtcgctacta caggaaacag aaacatcctc
     5281 aatagaaata cgtggtgtga ggtcgcctac acgaagctca agggatctat tggtacggac
     5341 gattgcagct tctttggggg tatctgcatc acagcagcat ccccaagaat cttcggaaag
     5401 aatatccagt gatgtaggcg catacatcat tctttttttc ttaccatcca taatgacttg
     5461 gtaaacacct ttgcccgacg tcgcattagg cattgtatac gttgagccca tatcaggggg
     5521 tgttggtttt tcaggttggc aggctgtcat taagaaacct gcggagataa atgtgacgag
     5581 tttaagtgtt gtgcgcattc gaatgctcct tgcaaagggt ttaacatgtt aagtctatca
     5641 tcaaaataaa aaattttaac taaatttttt atttcgcacg tccattggtg cccgcttaaa
     5701 actttttgat caaagtttac agcttgctgc ctcttgtgct tagtttcatt tgtacgtgaa
     5761 aaaagttaac aaacccttac taagagagaa aaatgtctat tattttttgg ttacgtcaag
     5821 atcttcgtct tttggataac acggctttgc agtatgcacg tcaaaaaagc tcggatgttc
     5881 attttcttta tatttttgag gaaacgcatg aggatgattt atccaccaaa ggcgctgctt
     5941 cacagtggtt tttatcaaga gctcttcgtt cctttcagaa aaatatagag gatcttggcg
     6001 gcaccctttt gattgagaaa ggcgatcctc tttctatttt agatagtgtg gcaaaaaaaa
     6061 cacgctctca aacaatttgc tggaacagac gctatgatcc ttataatgtg cgtaaagatc
     6121 aaaaaattaa gcaaactctt caagaaaaag gcattgaggt caaatctttt aagggatctg
     6181 tgctttttga accttgggaa atcttgaatc aaaagggaga gccttttaaa gttttcaccc
     6241 ctttttggag gcactgcctt gctaaaaatt ttgcgtatac accgtatctt tcgccttctt
     6301 ctcttaaaaa atcatccata gctcttcatc atgcgcataa aaaagacgct gtggattttt
     6361 cttatgatcc tttttggtcg aaaaactttt ctaaagtgtg ggaggatacc cccttcaatg
     6421 tcagtgagca aggggcgcat gaacgtttag atttttttct tcaaaaccac gtaaaggact
     6481 atcaaaaaac aagagatttt ccggctcttg aaagcacatc aaagctatct ccttatcttc
     6541 atttgggaat gatcagtcct gattttatta tgcaccgctg ttatcaactt ttatcaatcg
     6601 atagtgctta tgcagaaggg attcactgtt ttttgagtga aataggttgg cgagagtttt
     6661 cctcttatct tctctatcat tatcccgatc ttccaacatc ccctttgcgc aaagaatttt
     6721 taagcatgcc gtgggaggat aatccttctt tttttgacgc gtggaagaag gggcagacgg
     6781 gataccctat tgtggatgca gggatgagac aattatggca agaggggtgg atgcataacc
     6841 gtgtgcggat gattgtggct tcttttttga ttaaagatct ttttatggat tggcgtaagg
     6901 gcgagcggtg gttttttgat acacttgtgg atgcagacct tgcaagtaat tcagccagtt
     6961 ggcaatgggt tgcggggtgt ggcgcggatg cagcaccgta ttttcgggtt ttcaatccca
     7021 ctcttcaagg agaaaagttc gacccagaag gacattatat taaacgctat gtcccagagc
     7081 tttcattgat gcccgttcgc tttatccatg acccctcaaa agcgccacat tctatacttc
     7141 aaaaagcctc ggtggtgctg ggaaaaaaat atccttaccc cattgtagat cataaaatgt
     7201 gccgccaaaa agctttggaa atgttcaaaa atcttcgtgg aaaataaaat aaatgttatc
     7261 acgcctgtgt gacatgcaaa aatcgcatgc cttgctatga tattttattt gattaaaaat
     7321 acaaaattca atatatacaa tattgatgag tacaacatat taataggcac actctatggc
     7381 tgataattcc acgcgagcag aagctcttcg actttctgtt gatcttgtag gatcctatgt
     7441 ccaacgtaat gacacctcct ccgagcaact tattcccctg ctacaacagg tttattcttc
     7501 aattctcaat ttagcagaag gccacacgca tacattaagg acaggaaact taaagcctgc
     7561 tgtgccgatt gatcaatctg tttttgatga ttatattgtg tgtcttgagg atggtaaaaa
     7621 acttcagatg ctcaaacgcc atcttaaaac tgtctacaat atgaccgtgg aacaatataa
     7681 ggagcgttgg aatcttcctt ccgagtatcc ttctgtgtcg cctaactatg caaagcgccg
     7741 tagtgaaatt gcaaaaacaa gtggccttgg ccaaacaggt cgccgtcgtc gttttctcct
     7801 tgtgaataac ggccagcaat aatacctaaa aagtgaaaaa gggagcgtac ggctccccct
     7861 ttttttataa ttaaaaaagc ttttgaatga tcgttgcaat tttctgaaat gttggcttaa
     7921 tctcgttatc acgattatac ttcttaaacg ttgtgtcata aggaatgtat tcgaaattta
     7981 cagagtcata tttcggcaaa tcttctaaga actgtgagtg attatagtaa gattcatgaa
     8041 actgattgta aaaccattct tccataagag tatttttatt tttttcaagc tcttggttga
     8101 aaatcgcaaa ctgcttcacc tttttatccc aatcaattgg cgctttttta tggccgtaca
     8161 tcccccagcc actgcgacga atatgcgcct tatcatgatt ctgtaaagaa caagtggaaa
     8221 gatttttcgt aaaaggagct tcgtcataac taacaagttt gttcgtacca acggctgaaa
     8281 aaacagtggc gtgggcatta ccatttttgt caaaaaatcc tatacttcta ttcataagag
     8341 ttaaatccac tatgcctgta tcgctaataa cggggagata gatggcgtta agaatgagat
     8401 aatcccagtc tgaatccgta tctgtacctt gacggcctgg ttcataaaaa aactcctcag
     8461 ggttttcctt aaaagtttta gcggtttcta tctgtgcctt tttccatact ttttcaaaaa
     8521 catcaaagga aaaaagcttt gtccaagcat atctatagtc acgctctaat ttggcctttt
     8581 catattcttc ctcaccataa agcttataat tatcctcatc tttaaaaaaa ttaaaacgac
     8641 catctgaaat tttatcccag ctatcgagac tcaaagtaat gcttaaaaaa tccgcaaggg
     8701 ctgcatactc acgaaggttg agataaagtt tttcctcttc gcgcgcctga acccacgttt
     8761 ccatatcctt aatattttta agcatttgtt ggatggactc ttccttgcga ccatccttat
     8821 tttctttgag aacatcacgc ataaaatcaa cgttacgatt aagaatagta ttaatatcct
     8881 ttgtgcgcgt gtcattgcta aaattttcat ctttattgaa ttgatcttgg acgaactgaa
     8941 aaagctccgt gtctgttacg tattgagaag attttttctc cttctcctca ataatgttaa
     9001 tggtattaag atttttgacg tcttcgcttg caagaagggg tgttactgtg agagaaagag
     9061 caaggctaag taaaaaataa tattttttca tgattttata taaaatatat attgaatgct
     9121 ttttatataa ataatttttt atggaattca atctattttt tcatgaatta tgaatatttc
     9181 tagagctttg tcttaaggcg ctcacccgca aatccctttg cataaagaat atcccaggga
     9241 aaatgttttc cggggtcaat tttgcgcgtt ggtgcaatat cctggtgtcc taaaacatgg
     9301 gcccgaggaa tcgtatgcgt atcacataag tacgtcaaaa gctctaaaag tttttcaatt
     9361 tgaatgtcac tgtagggctc aaaaccatta ttatccagct cgatcccaag ggatgtgtga
     9421 ttcaaatttt cctccccctt ccacgcactc acccccgcat gccatgcccg cattgcgtca
     9481 ggcacgagct gataaatcgc tcctgttcgg cacaaaagat aatgggcgct gacctctttt
     9541 tgaggatcac acatccaatc aagcgccgtt tttgtgtttt caagcgccgt atagtgaaga
     9601 ataatccact ggatcgtagc gccttcgcgc cgttgatcgt ggtttgggga ggggttggaa
     9661 agaatatgca taggctttat ggccccttta gaagagagaa ttttaaaatt tcgagtgttt
     9721 ttctattttg accaattata gagagctctt ttaatatagc atcttggtga tgaacgttgg
     9781 cgaaaaatgc tttatagtag gaaaaaaagt aatttttttt gagggatttt cctcgcattt
     9841 tttcagaatt tcagattttt taggcaaaat tggggtattt tttgtgggtt ttcatgaaaa
     9901 ttcggtgaat tttcacgaaa aaaacgaaat aattttgctt tttttgtcta ttttttgctt
     9961 ttatgcccgt ttgcacatta tttaaacgat ttttggctat tttgagggat ttttggctgt
    10021 tttaagtctt tgattcttgg aaaaaatcat ctatccacca aatacagtga tttttttagc
    10081 aatattgtgc taatttcgcc cccatttatt aagattt
//
LOCUS       DAIAHG010000021        10078 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_21_length_10078_cov_3.092691, whole genome shotgun sequence.
ACCESSION   DAIAHG010000021 DAIAHG010000000
VERSION     DAIAHG010000021.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10078)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10078
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21_length_10078_cov_3.092691"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2891 a         2194 c         2100 g         2893 t
ORIGIN      
        1 acttaagtga agcccttcgc gatgaagcct ttatcacatt taaagaaaca gtcttaaatg
       61 ctctccaaga ggttgaaaac gcactgacca actttgcgaa cgaggacgaa cgacggatgc
      121 acctcaaaga tgctatgatc tccgatgaaa aagcgtatga gcttgtggat ggccgcttta
      181 gcgcaggcct tgaggatcag gagacagcgc ttcaggcaaa gatcacagca ctgcaaacga
      241 aaattaatta tctcgatagc ctaagatcac gctcactgga tattgtgagc ctttataagg
      301 cgcttggcgg tggttgggac accgcacagt ctttaaaaga cacaagcgcc aaggaataaa
      361 aaaacttatt tgtttccaaa aggaagaatg aggggaggaa ctgtctgaac aaagcaacga
      421 gtttccgtta cttctttgct actttctttt tctccaaaca gcaaaaatgt tcccatattt
      481 ttacccactg tatgcacgat gagggattga ttttcttgac ttttaagcac gtttctttca
      541 aaattattca tctcttcttc ccaataatca cgaatatttc tttgctctcc tcttagatta
      601 ttcaatgtat tgattaagtc tcttttttga tcctctatat cgcgcattat aaggggaata
      661 aaattgagag gatccccttt ctttttaaga ttactcatta ccttgtattt ttcaccaata
      721 tcttttttaa ttttattgaa cgtgtcatta taaataattg gcacataaaa tttcttaatc
      781 tctttatttt tgaattgttg aataaaaaat ttcgtgacat ttttattgag aatataaatt
      841 gattcagaaa atgtcgcctt agagggtgtc atggcgagca taaaaaggac aaacatctgt
      901 attaaaattt tcatttgaaa aaactttctt aaaaattgat acaaaatgga ttttggaaca
      961 tctttaaaaa gatggcaagc ggcttttgag tgttttaaaa tcaatactcc caagcggctg
     1021 atcctgcgct gcacaggaga aataaagact ttctttaccg gtttttggat ctacgttaca
     1081 ttggagacac tgcgcacaga cccccttcat catacattgc atcggcccat tcacacccgc
     1141 catcaatgtc acattctctt ttaaaaaaga tttcaaagac cccttaagca gtgtcgctat
     1201 ggcctctgtc atagacgctg tccccatcac aagaatatga tctatagtgc caagattttc
     1261 ttttatcttt tcaagtccat gccctgtatg cccattcaac aaataatcct gggggcgaag
     1321 agtgattgca tcggattgat tcaaacacca tataatatgg tcagatgcct cctcaatcgc
     1381 ttctttttta aaaatatctg ttcctttttg ataccctgca ataaatgtca cagtgcactt
     1441 tttctcatgc atcgcacggg caatgggaag caaaatcgca ttgccaagac ccccaccgat
     1501 caaaagaact tttttacctt cgggaataaa tgtcggtgcg cctgtcggcc ccataagact
     1561 cacagattcc ccaactttaa gatgctgaca cgcttttgag gacgcgcctg tttgcagcac
     1621 aatgagagaa attgtccccg ccacaggatc cacatcaatc cccgaaagag gcatagcttt
     1681 taaaagcggt gcgtcgctct tggatgcaaa gttttgaaaa cgaaagaact gtcctggttt
     1741 aaaatttttt acagccatag gggctttaat caccaattca agagcatttt ctgtaaggct
     1801 attcatttct ataatttcag cactcagttg cttttcaata gattctaaaa atttgtgatt
     1861 acgctcttct atctcatgct gtgacaaagt atgcaaatgc gttttttgct gtagtaattg
     1921 attaatagcg aggtatcctc tcttcgcact cgccatagcc tttacaacat tccccgcaaa
     1981 gcgcggatgc atatccccaa acattgtcat ggctttatga ttgttacgtt tatgaatcac
     2041 aaaaggagat atttctttga gcccatttcc ctctttttct gcaattgagg gctcgtcatc
     2101 caaaatattt gtattaggtt ctgtccccgt ggcaattaac actgtttttg caggaataaa
     2161 ttttttttca ccacgattat ttttaattag tacgcctgtg acggtatttt tttcatccgt
     2221 acacatatcc aaaagttctg cttcgtctat aatttttaca ccttgctgaa gcgcgtgctg
     2281 aacctcttca tgattcagtt tatacgcggg cgattcagtc aatgaacgac ggtaaagaag
     2341 tgtacaccct ccaaggtttt ccagaagtgg caagaaattt acatcacgtt tttcacgcac
     2401 agcgcactgt cgctcctctt caaaaagagt gtaatgaggg tgctgtggat ttttttgcac
     2461 atagcgcggg taatacgcca aagcttctgt ggctgtatca atagaggtta gtcccccacc
     2521 aatcacaatg acgggaagct caataggaag gtccgtttgt gtcttttttt gataagctcc
     2581 ccctaactgt agcgccatga gaaaatcact ggcataacgt acacctcgcg cgagggtttt
     2641 ttgataacaa tcattaagcg ttaaaagatg gggttttcca gcccccatac acaaagcaat
     2701 atgatcaaaa cccaactcat caaaagcctg cgaggatgtg agggtgccac caaaacgcac
     2761 acctcccata agtttaaact gaggatactc ctcgagaacg caccgaataa gttttaaata
     2821 gtttttattc caacgcaccg ttattccata ttccatcacg ccgccaaaac catcaatcac
     2881 gcgggtatca agatcactgc aaaaatcctt ccacgatgca atgggcttgt gagtagaatg
     2941 atgctctatt ttacttccat caattaaaac aaccgtatgt ccttcttgca gcaattgata
     3001 gctcagcgaa aagcccgccg gccctgcgcc cacaacaaga attttatgac cggtgagggg
     3061 cctggaaatt gggcgattaa agttcaaagg attccaccgc gtgagtaatt tataaatttc
     3121 tacgccatgg ggtaggttta aaatgctttc taagatttct gtttcacatc ccggaatatt
     3181 caccggtgtc tgtttttgga aaatgcacgc cttcatacag tcattacaaa tacgatgccc
     3241 tgtcgcggcc aaaagcggat tgtcgatcat tgcaacggca agcgctccca aggaagaacc
     3301 ttgcattttg agcgtgttca tctctgaaat tttttgatcc aacggacacc cttgaagggg
     3361 aatacccaaa ggattttttg taaaattttc gctcacttta tccttaaaac cctttgaaca
     3421 tgtgtcttta tcctgcttat gacaatgaat gcaatagtgc gcctccgcca aagattcggg
     3481 caacgaaaag cctttatccg tgagggaaaa accatcacga ttcatgcacg gattttcagg
     3541 ttttctgagg agatgatcga aatcaatttt cacaggtgtt tgaaaaagaa cacttttttt
     3601 atgtttttcc ttcccttccc ttgtatgcac agcccaaaca caataaaggg ctgcacgctt
     3661 taatgcttct tcgttgtgcg ttttaagaag accctcaata aaatgtgcac aatcaagatc
     3721 aaaagactct tttggaaaat caagatgagg ctgataactg tcaagatccg ccacatcata
     3781 actcttcaga gccacgcgct ggacaaattc ccgtttaatg cggtaaataa tcgcttgttt
     3841 tgagtgagcc tcctgaaggg ctttgagctc cttctcaatg gaaaaatgct ggcctataaa
     3901 ttcttcaaca aagggcgcaa gctcaatcac acatgctgat tcttcagctt ttgaaagttt
     3961 ttttggattc tcaagagcct gcgtataaga cagataacgt accttatcta ctttttctaa
     4021 aaaatctaaa aagtgtcttt gaatataatc aatatgcgtc atggttttta ttcgttatat
     4081 tttttttgcc acgcctggta cattaaaatg ccccacaaac tgtaggatct gtcatgtttg
     4141 aaagacatat gcgcatcgag acgctttaaa atcggatgtg tgtctaaaaa accattttcc
     4201 tcgaggtgct tttttgacat taactccatg gcccagtctt taagagggcc gcgcagccac
     4261 tgggcaatgg gcacaccaaa tccctgttta gggcgatcga tcaaattttt cggaacatac
     4321 tgctcaagaa tgcggcgcaa ggctatttta ccgatgccac cctgcaattt taaagacata
     4381 ggaagccgcc aggctcgctc aatcacatga tgatcgagaa ggggctcacg ggcttctaag
     4441 ctgtgtgcca tactcgcccg gtccacttta gtgagaatat cgccttcgag atacatgagc
     4501 gcatcacaca actgcatctg atcagtaaaa gtgcgtgcgg attttgtttt ttcccacaca
     4561 tttttaaaat gttgcaagga ttgtcctgtc atctgatgga tttctttttc atcccaaagt
     4621 aaatacgtgc tgtgaaaaag agattctcca ctgttagtgc ataaaagatc cgaaagtttt
     4681 ataagtttgt ctgctgggtg agaaagatgc agcgcttttt caatcattgt gtggagaatg
     4741 ttataacgac tatgggctgt atgcttcatc agtggcacta aacgtttgcg caatccctca
     4801 ggcactgttc ctagataacg ccacaatttt tcacatatac ggtgacgggt atagcccgca
     4861 aaaaattcat caccgccatc ccctgaaagc gccaccgtca catgctctct ggccattttt
     4921 gaaacaagat atgttggaat ttgcgatgaa tccgcaaagg gttcatcaaa aaaatccgtt
     4981 aaactcggaa taatatcaag tgcatccttt gacgataaat agaatgtgtg atgatccgtt
     5041 tttaaatgcg ctgcaacagc ttcggcataa tgcgcttcgt tgaaacggtc ctcttgaaaa
     5101 ccaatcgtaa atgttttaac gggtgctgcg tttgcttctt gcatcagtgc caccacaagg
     5161 gagctatcca tgcctcctga taaaaatgcc ccaagaggca catccgcaat catacgcaaa
     5221 cgcacagccg cgcgtaacgt ttcgtgcagc tgatcgattg cttctttgtc attttgaaaa
     5281 ggagtcgctg tgtcatagga tgcagtcata tcccaataga acgactgtgt ttgattaaaa
     5341 tctgcatcaa aacttatcac agaacccggc gtgacttttt gaatttcacg gtagacggac
     5401 atatcgcctg gaatgtaatt atacgctaaa aactctgcgc gtgcactttg gctcagcgtt
     5461 ggattccatt cagggtgagg ataaaaagag cgacactgcg accccaaaaa aagagtgcgc
     5521 ccaataaccc cataatagag aggtttcaca ccgacacgat cacgaactaa atgaagtttt
     5581 cgctcatggc gatcaaaaac tgcaaaagca aacatccccc taaagtgttt aatcgcctga
     5641 agaaggccat cgtgttcaat cgccgccaac attacttctg tatcagaatg ccccttgaaa
     5701 acataacctg ccttttccaa atgaggccta agagccattg tattataatt atagacctcg
     5761 ccattaaatg tgatgacata acgacccgat ggcgacacca tgggctgatg accggcttct
     5821 gaaagatcaa ggatcgatag tcgcctatgg gcaagggcaa gccccgcctc atcgtctacc
     5881 cataggccaa aagaatcggg gccgcgaaaa cgtatttttt ctgacatcgc caaagctgtt
     5941 ttttcaagtt ttgtatgctc ccagcgatct tttgtccaaa aacctgttat tccgcacata
     6001 gtgctgcaca ctttatttat gtattatttt ttaaaatcaa tgcatttttt aagcatccct
     6061 gcaagcaaaa gttaaaaaga atcgattgtc tttgatgggg cctcagaaat atggtagcat
     6121 gagaagaatt ttgaggaaac attagacaaa tattaagaca atccttatat gctatttaac
     6181 aagagccatt ttcacttcaa ttaacccagg tccatgcgaa tgtatttaaa aaaaatccaa
     6241 gatattttga aaaaagccat taatcatctt aataaaggat gggatgcttt catcaatctt
     6301 tccgttgtga aaaagataaa acacagcgct atcaaagcct ttcattttat tgatcgctat
     6361 atcaaatttg acaagcaacc tacaaaaaca aaagcaggta ttgtttttgc aggggttgct
     6421 gttgggctta tcattctttg ggtgattgcg cgtttaggcg gacttatcgt ttataatgcc
     6481 tgtgttgtcg tggttgattc gggggtcaaa tcacgcttta tgaagcgtac aaaagcttct
     6541 gctattgagg ctgcaaaagt cgttcctgga tccatttcac gccgtattca aacggtgggc
     6601 caacttcgcc ctaataatat cgtgacgctc aaatccgagg ttaatggccg tattacggaa
     6661 attcctgtaa aagaagggag cgaggtcaaa aaaggcgatc ttctgattca gtttgacaac
     6721 gcagatgctg tggctgagct taaagatgct gaggccacac ttgccctaaa agaagctgat
     6781 tttaagcgca tggaagccct taaaaatgtt gtggctacta acaaaattga tgaggctcga
     6841 gccggttttg ccatagcaga ggcaaaagtc gcaacagcta aagcacgcct tgaaaaaacg
     6901 caaatcagag ctccttttga tggtgttatt ggtctgattg agcaaagccc tggaacctat
     6961 gttcaggcag gcacagaact tgccacggtg gttgaaaata atccaatgaa ggttgatttt
     7021 aaaattcccg agcgcaacct tcacgaggtt ggtgtgggcc aagcgattga tattagactt
     7081 gatggatttc ccgatcaaat ttttaccggt acggttgaag ccattgattc taaagtcgac
     7141 accaaaagtc atagtattgc tgtgcgaggc accattccta atccaaagca tctgttacgc
     7201 ggaggtgttt ttgccaacgt tagccttatt gtgggtgaga agggcgatgc gctccttgtg
     7261 ccagaatcag ccttagagcg tgagggcgat atcgaatatg tttgggttgt caaacggggg
     7321 aaagcaagcc gcaagagagt tcttgtaggc tctaaggaaa ataataaagt tgaaattatt
     7381 actggtattg agcctggatc gattgttgta acagcgggtc aaacacgctt tgcgggggat
     7441 ggacaccctg taaaaatact caatttgaat gaagacggga ctgaaaaacc tgaggctgag
     7501 aagaaaaaaa tcgaggacgt tagtgacgaa gacgatgatg agtagaatgc tcacgatctt
     7561 ccgccctaga aaaaagctga tgcccacgca atagatatag ataaagagga cgccctttta
     7621 tgaaaatttc tgaaatttgt attcaacgcc ctgtccttgc gtgggtgttg acttttctcc
     7681 ttaccattct tggtctgatt gcaggaagcc gtcttccttt gttgcaatat cctaaaacag
     7741 agcgccccta tgtcacgatc gaaacaagcc ttctcggtgc agcgcctgaa attgttgaaa
     7801 gtcagctgac aaaatttatt gaagaagcca tggcaggtat cgaaggcctt gaggaaatta
     7861 cctccacaag ccaagtagaa gatagcaagg ttatgcttga gttcatccca ggctattcta
     7921 tggaaacggc aacgaataat atccgtgatc gccttgccaa aaaagctgat actctcccag
     7981 aagaggccac gcgccctatt ttgacacagt cccgcgcgga agaaaaaccc attattaccc
     8041 ttgccctgac gagtgataca ttgcccgcaa gtgaacttgc tgactatgca gagcataatc
     8101 ttcaaaagga tattgaatcc atcccaggtg ttgcgagcgt tcaagtgctt ggtgcagggc
     8161 attatgtgat gcacctctat ctcgatccag tcaaacttgc agcctacaat attacagccc
     8221 acgatgtccg cattgcgata aaacgcaata actttgataa acccgctgga aaattgattg
     8281 gcaaggaccg tgaatatatc gtcacaaccg tcgcaagtat tgaaacaccc gaggagttta
     8341 atagtctcgt tattgcagag cgggaaggac accttgttcg gctgagtgat attggttatg
     8401 caaaggtcac cgatgataac acaaaaacaa aaacgattta taatggcggc gaaggcgtca
     8461 gtattggcat tatcaaacaa tccctctcga atccgcttca ggtttccgaa gatgttcaaa
     8521 agtggatgga gagtgcgaaa aaaaatctcc ccgaggggat taaaattcat attggttcgg
     8581 ataaaacaac gtttatcaaa cgctcactgc atgaggttta tcaaactatt tttgaagcaa
     8641 ctcttcttgt gattcttgtt gtttttggct ttttaagatc catgcgcgca tcgattattc
     8701 ctcttgtgac gatccctgtc tcgcttattg gtgctctttt cctgatgtac ctccttggct
     8761 ttagtattaa cacactcaca ctccttgctc ttgtgcttgc ggtggggctt gtggtggacg
     8821 atgcgattgt tatcttagaa aacgtttatc ggcatattga aatgggagca acgccctatg
     8881 ttgcttcttt tcgtgctgtg cgggaaatta gttttgctgt tatcgccatg acactgactc
     8941 tagccgctgt gtatgccccc atttctcttg cagaaggtat gattggaaag ttttttaaag
     9001 aattctcgct cacgcttgcg ggggccgttg ttatctctgg cttttctgct ttaacacttt
     9061 ccccgatgat gtgcgcacgt cttctaaagg cccatgatca taaaaaaccc aaaaaaccaa
     9121 cggcaggatc ttcccttcgc gataaattaa aatacacatt cttcagcgat gtatggctcg
     9181 aagttatcga gggattttat gaaaacttgc tgaaaaaaac cctcaaaaaa cgtgtgcgta
     9241 tggcgttttt agggctgtct tttgcagggc tcggtggtgt tgtttatctt ttgcttccaa
     9301 gcgaatattt ccctcaggaa gatcaaggct ctatttttat tgagggatcg gctccccact
     9361 cagcaacact tgcctatacg gatcgttatg tgagtcaaat cgataagttt cttggcagct
     9421 tgcctgatga tattgaaaag cgcgtgacgc aaattaataa tccaacctat gacattagtg
     9481 ttcagcttaa ggcggaccgt aagcactcta cggctgaagt ctcggagatg attaagaatt
     9541 ttatgtctaa tgtcacaggt attgaaggac gtgttgaagg cggtggtggc ggcagtggca
     9601 gcagtgataa atcgaactct gtcgaattcg ttgttatggc aggatcaaag tcttacaggg
     9661 aactaaaaga gatcgcaagc aatgtggttc aaagttgcta tcgctcgggc attgtggata
     9721 tggtcacctc agaactgagt aacggtgata cccaagatta tacaattact cttgtgcgtg
     9781 acaaaatttc agccctcaat atcgagcctc tcacgattgc agaaatgatc gaaattttga
     9841 tgcggggtca aaaagcaggt cactttaaaa aagataataa actttatgat gtacgtgtcg
     9901 aggttgagga taaaaatcgt caaacaccgt atgatattac aaatcttttc gtgcgctccg
     9961 gcgataagca aggcacgttg attccgcttt ctgaacttgt gactgtgcgt gcgcgttcgg
    10021 gccctgtgga aatgcatcac acaaatcgcc aacgctccgt cactattcgt gcaatctt
//
LOCUS       DAIAHG010000022         9993 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_22_length_9993_cov_3.333300, whole genome shotgun sequence.
ACCESSION   DAIAHG010000022 DAIAHG010000000
VERSION     DAIAHG010000022.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9993
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22_length_9993_cov_3.333300"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    2671..2680
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3027 a         2192 c         1966 g         2798 t
ORIGIN      
        1 aacttgagct gctgactcat aacaaattct tgtgcattcg gagggaggat cgccaaaagt
       61 ttttcacttc cgttttgtac cataggcata aagcgtgcat ttttcagtac gtctggatat
      121 tgagagcgtg gcataaagct actgagggca agttctaatc cacaggcaat aatcattgca
      181 cgaagaatcc caaaaccaaa gccaagggag cgatccaccc cgccaagggc actgtctttg
      241 acataggagg aaataatatg gctgatcaga ctaaaaaaga taagaaaagt aataaaaata
      301 acaaaagccg ctgcgacatc tgcaatcatc ggattttgaa tatattgctg agcaaagtgc
      361 cgtgcgacgg gaagtaaaat ataggcagct acacctgcac caatccagga cacaaggcca
      421 agggcctctt tggttagtcc gcgccacacg ccaaggaggc tagagagggc aaaaataaca
      481 acaaggccta tatcaatagc attcatacaa tgaaaaatat cccaaaagca ttaaaagagc
      541 ataataacca tttttacatc tttttcaaca tacgccaaga gaacattcag aggaaatgaa
      601 aaaaggctcc cgcacttttt actctaaggc gttatcttta gattcctcca aaagagtcag
      661 ttccgttcgc ctactttgtg tacccgcatt aacaatagtc tctacccatc tatattcccc
      721 atattcatgt acgatgctct cttcttcccc ctttttaaat ggcgtaaagt ggctatcaat
      781 gaacactttc cgtgtgcctt tttctttttt ttcatcaaat tcaatattaa atttcttttt
      841 catgcattcc gttaaaacaa aggccgaaaa ttctaagtga tcaacaaaat catcggggta
      901 attagggtga tccccctctc cacgcacatc ttttaatcga agtaaacttt tattattgta
      961 ggggctttcg tttggtgcac cacattctgt gacatacttc ataaatgact tactgagggg
     1021 aatagagccg aatttatcaa aatcttttga tgagaaggca atcgtatttt tcttcttttc
     1081 atagttaaac gttttatctg aaaaaatgtt gcccattata aatgtgggcc aacctttctt
     1141 tgaaaagcat ctaaggaaaa ttgttttatc ttccgtctta gtctccttta aatcctctgt
     1201 cgcatgtgtt tttcccacta tcgcaataaa ggctatggaa aataaaattg ctttcaatat
     1261 gttgtgcata aaaattcctc tatttaatga ttaacagaag ggaacgttag cataacactt
     1321 atacgtccaa caattatctt aacgtagttc cttaagtaag tcactcagat gattcaaaga
     1381 gatgcgctga aggggtgagg ccaccttgtc cagaccaaag taagcacggg cgaaccctaa
     1441 cttttccgct tctttgagac gtagattcgc ttgttgaaca cggcgcacct caccacccag
     1501 ccccacctcg ccaaaaaaca cagaatcttg gggcagtggc ttttttaaat gagaagagat
     1561 cagcgctgca gccaccgcaa gatcgcacgc tgtttctgtg attttcagtc ctcctgctac
     1621 attcacgtac acatcttttg tcccaaagct tatgttaaca cgcgcctcga gcacagcaag
     1681 aatcatggat aaacgagagg gttcaatccc cacggctgtg cgtctcggcg atgggagata
     1741 gcttggcgcc acaagtgttt gaatttcagc caagagcggg cgcgatccct caaggcccgc
     1801 gtaaatggcc atgccgatag acgcatcacg gtgatcgcct aagaaaagct tcgacggatt
     1861 cgagacctcc tcaaggcccg tttccaccat attaaaaatc ccaatctcat ccgttggacc
     1921 aaagcggttt ttaattgcgc gcaaaatacg gtaagggtag tgcttttccc cttcgaaata
     1981 aagaactgta tccaccatat gctcaagaac cttgggccct gcaagcgccc cttcctttgt
     2041 cacatgcccc acaagaacaa tgataatatt tttttctttt gcacactgaa tcaattcaaa
     2101 agcggacgca cgcacttgcg ccacactgcc cggcgctgat tcaagactgt gcgttgccat
     2161 ggtttgaatc gagtcaatca caagaaattg cacatcctgc gcggcggctg ttgtactgat
     2221 aatatcactc accgaagagg tcgccgccaa aagcacaggc gctttatcca actgcatccg
     2281 gtgcgctcta aggcgaattt gatccacgcc ctcctcgccc gaaacataca gacacggtgc
     2341 cttatgactc agtttgcttg ccacctgcaa cagcaatgtt gattttccaa taccaggatc
     2401 gccgcccaca agaatcacag aacccgggac aaggccgccg ccgcacacgc ggtcaaattc
     2461 ctccatgccc gagacaaggc gcgcaggtaa ggatgtcact ccctcgagag acacaagttc
     2521 aagggccgcc gtaggatttt tttttaaaga atttttcgcg tcgacaattt cttcgacgag
     2581 gctattccat ccgccgcagc cctcgcattt tccgttccat tttccgtgcc aggtgccgca
     2641 ttcacggcag acgtattttg ttgcaagctt nnnnnnnnnn tttgccatga ggaaagagat
     2701 ttattcaatg aatttctctg actatagcaa cacccccttc ctttcacaat tgtctcctgt
     2761 gaagaggggc ccccgtagga gcccggttgc gttagttacc aagcttttcg aggtcatttg
     2821 taaaattgaa aaaaagatgt ttctttttat tttgcccatt catgtttttt atatcctcaa
     2881 aaatatcacc cgccgcagcc atgtctatga ttttctgaat atctttcttt tttatcttat
     2941 tatttttaag ggcaagcctg atgtcatctt gtattttttt tgttaactct tcgagctcat
     3001 ttacgctcat cgttcctgtc aggggatata caccagcgat ttcggttgcc cactcctcta
     3061 ttgtttttgt agggcctttc atttcttcaa gtttattctc taggatattt ttttctattt
     3121 caaaaaaatt ttaccatttt tttcgcattg tttatatgtt tttgaagatg tttaatttta
     3181 ttctttatct cgtccctttc acctgcgaag cttacgcccc ccccccaata gcataaatgc
     3241 tgtggtggct gcaataattg tgttaaatcg tgtcatggta caatgtcctt tgtttaagat
     3301 gacctacaaa ggagaatgta ccataaaaac gttaatataa agtaaaaatt aatacacaga
     3361 ttttataggg ccctcgcgca gaccatgcac aaattggtgc aggtacgcat tatccgcctc
     3421 gtccaactcg cccacgtccc cctgccacat aatctttcca ttatgcaaaa gggcaaccct
     3481 gtctgcgatg gctcgcacac tgctcatgtc atgggtgatt gtcacagagg taatcccacg
     3541 cttttgtaca ttttcgcgaa tcagatgatt aatcgtgctg cttgtgatcg gatcaagccc
     3601 tgttgtgggc tcatcaaaaa aaataatttc tgggtttgac gcaatggcac gggccaaggc
     3661 cacacgcttt tgcatgccac cagaaagttc agaaggatag cgatagagtg tttccctctc
     3721 aagattcaca gcttttaatt tttccgctgc aagggtcatg gcctcttttt tattcaaaga
     3781 acgcccctgt gcccccattc cctgaagcgc cataaaagca atattctccc acacgggtaa
     3841 actgtcgaaa agagcgccgc cttgaaaaag catgccgaat ttgcgcatat atttttgacg
     3901 tgcgccggaa gataactccg cgacttcatc accatcaatc acaatagatc cgctgtcctg
     3961 ttgaataaga cccagaattg ttttaatgag cacagatttt cctgtccctg aggccccaat
     4021 aatgacaagg gattcccccg tataaacatc aagactgata ccatcaagga caaccttgtt
     4081 atcaaaactt ttacaaaggt ttttgatctg tattttagat attttcaaag tcatttttca
     4141 ttctttttta acggttgatc tttatcactt aatacgacag ggattaagaa ccaaggaatt
     4201 gcatacccat gataaagata ttcacagcaa caccttttat cataataact ctaattatca
     4261 acatgcaagg tgtattcgca tgctccaatg cctctgagga tgagcttgag caacgaaaaa
     4321 tctcacccgt gcaaaagaaa atagattcta ttcaggatgc actggctgcc cattcttttc
     4381 taaggcatca caaaaattcc gggtgggaga taaatgatct gctttttcaa cccgatgatg
     4441 ttttaaatac ccacggcatg acaaaaggat ccttttttta ttttgctgga tgataactat
     4501 gaggaaaaaa ttgaagaagc tctcacgctt caaaagcaat accacgagga ggcttacgat
     4561 gctcttgagg attataaaaa tctgattcaa gaacttgata aaaaaattag ctctacttac
     4621 gcctgcaata cctgccgctg tgttcctccg cttattgaaa taattataaa aaacgcagaa
     4681 gacggtatgt caacggagca agttttgaaa cttattgctg agtcttttaa taatacactg
     4741 aaaactcaaa aaaaaagcag aaactagagt ggaagccgag actgagtata gaaaaaataa
     4801 cttatttggg tattttaagc caaaagtgct catcctatct gccttaaaac agacaaccaa
     4861 agaaaacatc gagtatttca aaaaagcgcc ttagagaaaa cgtgagaaag agttttccga
     4921 attccggcgc caaatgtgga tttatgaaca agagagaagg cgcagagaaa cgtataatgc
     4981 aatgtgggaa aatcttttaa aatactttaa aaaataatta tcgggatcac actattgctt
     5041 ggtaaacaaa agagcggtca gggcatagtt aaaaagtaaa ataagaatcg acgcactcac
     5101 aaccgcgttc gtcgttgcct gcccaacgcc tgccgcccct ccttttgagt gatacccgtg
     5161 ataacagccc ataagagcga tgataaatcc aaacacggcg gccttgataa gacccgagat
     5221 cacatccttg gcttctaaaa aactataggt ttggtgcaaa taatttcctg cattaaaatt
     5281 aagttgatag acacttacta aatacccgcc catcacacca ataatatcgg cgatgaggac
     5341 aaggagtggg aggctgacga gtgctgcaag cacacgcggc acgataagat actgcatagg
     5401 attcgttgaa agtgtcctaa gcgcatcaat ttgttcggtc acacgcatcg ttccaagctc
     5461 tgccgccatt gccgcaccca cacgtcctgc gaccatgagt cctgctaaaa caggaccaag
     5521 ctcccgcgtc atcgaaagaa ccacaaccgt tgcaatagca ttttctgcat tgaaacgtga
     5581 aaaacctgta tagctttgaa gcgctaagac cattcctgaa aaaatggctg taagaccaat
     5641 cacagggagg gacaaatacc ccacatgcat cagctgatga aaaaattgct taatgtgcca
     5701 tggtggcaaa acaatcgcaa agcatgtctg aaagaaaaat aagcccactc ttccaattgc
     5761 agcaagtgtc ctaagcgtgt gatgccctac ccattctaag atataaagaa gagacagagc
     5821 atatttcatt attcaccctc atgatagtga cggtggaggc gcccacccag tttaagcata
     5881 atttcatacg gcaacgtcga tgcccacttt gcaaggtcat tcacactaat agtctcattt
     5941 aaaatatcca caatagcgcc ctcataaatg tattcacttg gaacatctgt cacatcaatg
     6001 gtgataagat ccatagaaac acgcccgaca ataggcgctt tataggcacc aatcatcaca
     6061 aaaggctcat gccctgctgc acctaaattc catgcaaaac catccgcata gccaagtgtc
     6121 actgtcgcaa tttttcggtc agattgtgtg aaataacttt gggcataccc aatgctttca
     6181 ttttgtggga cggtttgaat ttgatagata cgcgcccaca gacgcaaagt tgacttaaga
     6241 cctttattgt aagacgtagc acacccataa agcgccatgc ctggccgcac catatcataa
     6301 taataatcgg ggccaagaag gcagatgcca cgagagttcg ccaaacttaa ttgcggcttg
     6361 gatggtaatt ttaattcttg tactgctttt gtaaaacgct ttcgttgaag ctcattcaga
     6421 ggattatttt tatcatcaga gcaggccaga tggctcataa caatctgaat atccaacttt
     6481 gagagactgt tttctaaaac ggagtggctg aggcgatcaa aacgaagccc tgtgcgattc
     6541 atgcctgtat caagatgtaa aacagtgggg agtgggttct gttttgaaaa agacgcccac
     6601 ctttcgacct tttcaatatc agtcaagaca ggaataaatc cgtgggcgag aaattcctcc
     6661 tcttgcccct tcataaagcc attgagaacg taaattttag tctcatgatt tttaatatcg
     6721 gaaggcgcat agcccaagag atttttttta agttcgatgg cctcttcaag atatgctgta
     6781 aaaaaatggc gacatcctac atcataaaga gcccttgcac aggggccaat cccaaggcca
     6841 taggcatttg cctttaccac ggcactgcat aaacttgatc ccaggaggct ttgaagtgtt
     6901 ttaaaattat gttgcagcgc ctgaagatca atcgacaaac cacaatgatg ataatggccc
     6961 tcttcttgcc gtggaaaggg aataatcgac gctgtgatcg ctgggttaaa agtcattttt
     7021 tctcattatt ttttttaaaa gattgttgat tttttatcgt cttatttttg aacagtctac
     7081 tacaatacgg acagacaagg gtatctgttt taaacgttaa atagattctt ggatgccccg
     7141 atgcatcgcc cagttctcca ctttcaccgt cacaaaaaac ggaggattca tccgtataaa
     7201 tgatttcttt ttcatgcatg aggggcgtcc ctatcaattt caattggctt cacctttatt
     7261 ttaacgtata taaaaaagag gttttgtaca aggcacccgt agctcagctg gatagagcgt
     7321 tgccctccgg aggcaaaggc cagaggttcg aatcccctcg ggtgcgccaa aaataataag
     7381 tgacaagggt tcatcccctt tttctaccta gagacctatg tatgcagagt aacacgcatt
     7441 tattaaaatt tctttaccaa gcggggttga attattcttt tttagagtcg ccccgtaatc
     7501 ggctgactga aaaagaacta tcgctcaaag agcctctatc agaaaagaga tctatgacta
     7561 ctcaaaaacc atccatttct actcctgcaa aacctttgga aacacaaaag cacgttgaac
     7621 atcccgccta tgcatgtcaa accctcgacg atttgcgcaa agcccttgac gtttttgaag
     7681 gatgcgcgtt aaagcaaagt gctcagaaca cagtcttttg tgatggaaat ccccaaagcg
     7741 atgtgatgtt gatcggcgaa gcaccaggcg ccgaagagga taaacaagga aaaccttttg
     7801 tcggcatgag cgggcagctc cttgataaaa tgatggcggc catcggcctt aaccgagaaa
     7861 atgtctatat tgcgaatatt attccttggc gcccgcccgg aaatcggccc cccagcaccc
     7921 aagaaattgc agcatgcctt ccctttattg agcagcatat ttatctcaaa aaaccaaagt
     7981 ttctgttact tttaggttct gtagcgacga aagcgattct taataatcat acagggatca
     8041 tgaaattgcg tggtcagtgg caaacctata agccgcctat ggcgcacccc aatgaccccg
     8101 ttattcaagc actgccaacg tttcaccctg cttttttgct taggtctcct ggtcaaaaag
     8161 tttacgcatg ggaagacctt aaaacactca aagccgcgat ggataaataa aaaagcactt
     8221 gaaaataaag tttttattca ctaaataatc tgcatagtca atatttaata tatacaataa
     8281 gattatgtca ttgaaaatta ttcttgcatt aacattaagt ttatcctctg cgattgcatc
     8341 gactgaactt ccaaaagaat ctctcgatct tttcggggac ggtaacaaga aggaggtaaa
     8401 actacaatcg ccttatcttc atcatgaaaa agacatgaat tattcgctta ttacccaagg
     8461 cggctataaa gtttttgaca ttgatcctga gtccaaagac gttgctgtga ttgggccttg
     8521 tcagccctgt ctggggtttt ttgtaacgga tggatcaaag gggattgctg gccatagaca
     8581 ctggacgaat gatctaaacg atcttggcaa gattattaaa aataacctaa ataccgagga
     8641 tccctcaaag ctttttgcct atatttatac agtcacaatc aatgacgagc tgtataaaaa
     8701 aaggatacga aaaaggttat ggcggctctc atacagaagc cgtcaaaaag ttaaaggatt
     8761 atttgtgcga cacattgaat atcaaggatc gcacacaaat taaagcacaa ctttatacat
     8821 ctggctataa agatagtgcg cttggacgat atgatacaac agaactccat attgcgatta
     8881 aaatgagtga ggttaagaca gggaacgcga ttaacgttta ttctattgat ccgtatgtca
     8941 atgatatttt gaaccttact ggtacaaaaa tagaagcgga gtatatttat ggcgatagag
     9001 ccaacataag ctttgaggat tataaaaaaa tgcttccgat agaatttctt aaaaacacgt
     9061 cgaatgaaga tattgagtca gagtataaaa acttaaaaaa gcctatgctg tatgatacca
     9121 ttgaaaaaag caattggcgg gttgcaagag gcgtttatca gagaaaaatg caaacgattg
     9181 aggataacag atatacggaa aaatttggag aagttgcaca aaaactaata gatgacacgg
     9241 gtataagccc cgaaaatttc cgtggatcgc tcccttttta taaaataaca gaataaaggc
     9301 gtgcgattaa ccttatgtat gctctcgtca ggatcattgc attataaaaa ataatactcc
     9361 ttgttagttc attcacagtg caattaaaag atgagatttg cattgaatgc acaacagaat
     9421 tttttgggga gaaaaatcct caattaagat ttttgcttct tgatttaagg gcgatttcat
     9481 tgctattttt tgattttaat catctctatg cgaggggagg caaaagagaa aaaataacga
     9541 gatcatgcat agataagggc taaaggtgct gaaaaaattg cttttttagc ttttttccgc
     9601 ggggtatgac tatttattct catttttcgc caagaatttg cattttttag cgtatcgagg
     9661 gctcattttt atgtggtttc atgtgcttat tatgaaagat aacctaagta tcgtgaaaaa
     9721 atcgcttttt taagggaatt tagcacacta tccctgaaga aaatatgaga gcttaaatct
     9781 tgagagaaaa gtcatttctc tttgaagaaa ttgttggcat tggtgtgaaa acagacattt
     9841 tgtacgcctc aatttttggg ttttctgctt tttctgcgat cattttaagc gagactgtgc
     9901 gaagattttt gaatgacgct gtgtgtggag aatttttttc ttttaaaata acagaaaatt
     9961 ttgcgggaag aatttttttc atgtgagcta tgg
//
LOCUS       DAIAHG010000023         9789 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_23_length_9789_cov_3.431219, whole genome shotgun sequence.
ACCESSION   DAIAHG010000023 DAIAHG010000000
VERSION     DAIAHG010000023.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 9789)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9789)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9789
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23_length_9789_cov_3.431219"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    241..250
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2932 a         2079 c         1913 g         2855 t
ORIGIN      
        1 agggggaaaa tatttttcgc tccacaaagc gtttgtttga tcatcccaca caaaaacttc
       61 aaggcaccga cgcaaccccc ttaagtattg ccacatctct tttaaaagaa cttaaagaaa
      121 aagcggaaaa agcgttagaa aaaaccgtca cccatgcggt gattaccgta ccggcgtatt
      181 ttgatgaaaa ggcgcgtcag gccacaaaaa aagccgcaga ggacgccggg ctgacagtgc
      241 nnnnnnnnnn cgcagaacct acggccgcag ctcttgccta tggcttagac aaaggggttg
      301 aagggcttta tgccattttt gatttaggcg gggggacgtt tgatttttca ctcctcaaac
      361 tgcaaggaga agtgtttcaa gttcttgcca cagggggtga tgtgcgcctt ggcggggatg
      421 atatcgatgc ggcgatttgt gagcactgcg ccttttctga ccttgagaaa gcacgcctta
      481 tcaaagaaag cgtgagtgat tctttgaggg gtttttataa taatgatgat cagtcgaata
      541 gtaatagcgg cgatagaaat aatcacaagg caaatacttc aaactcagag tttcctctca
      601 gtgcggatga ccttgaaaag ctttcacaac cttttattca gcgcgctttg tccatttgcc
      661 gtggggttct aagagatgcg cgacttacgg tgcgtgatat tgaaggtatc gttttagtcg
      721 gaggatcaac acgccttaca tgccttcgaa aggccgttaa agattttttt gagcgtgccc
      781 ccttaataga tattaatcct gatgaagtcg tcgcccacgg tgccgctctt caggccaatg
      841 cgttgaccca tggcggaaac atgctgctca ttgatgtgac gccgctgagc cttggaattg
      901 agacgatggg cggtcttgtt gaaaaaatta ttccacgcaa tacccccctt ccctgccata
      961 tggcccagga ttttacaact tctgtggatg gacaaacagc gctgatgatt catattgttc
     1021 aaggggaacg ggaatttgtg aaggattgtc gctctttggg acaatttatt ttaagtggta
     1081 ttcctctgat gaaggcccat agtccacgga ttcgcgtgtc ttttcaattg gatgctgatg
     1141 ggcttttaac cgtaagtgcc cttgagcaaa caacccaaac gcaacaagtt ttaagtatta
     1201 aaccaacggt tggcctcgaa gaggaaatgc ttgaggaaat gctccttgat gccgcatccc
     1261 acggtgcttc ggacatggag gagcgtctgt ttactcaaac gcgtcaagag gccgaagaaa
     1321 ctcttttacg ccttaacaat gcattaacga ctcttcacat ggttttggag gactctcctg
     1381 agcttttaaa cgcggtgagt gctgttgagg acgcgattca cttaaaagac agagcttccc
     1441 ttaaaaacgc cctaaaaacc ttaaatagtc tctgtgatcc cctggcaaat caggtgattg
     1501 tgaaaatact gaaggactcg taaaatttta ataaaaataa taataaaatt ttatcaaata
     1561 aatctttcaa atatatgcat ttttttcttg aatattgact aaatactgct tagataaagt
     1621 gtatcgaaaa ctctaagcaa ggatttagac atgttaacaa aaaaagttac cgttctttct
     1681 gtcgctctga tgctctcggc tactgcaaca tctgtccaag cggactggtc ttttagtggc
     1741 ttcaaagaga ggatttttgg caaaaagact actgtacaaa aagaagaagc tcctcttgta
     1801 aaagaaacag agctcaaaga cgtcaacgtt aataaaaaca ccaacgttga tcaggaagat
     1861 attttctctg acgagatttt ctctactgaa aaagaagtca acgcaacact taaaaaagaa
     1921 aacgaagaaa aagcactcgt tctttacaac gaaaagcttg acccttctgt aaaggttgaa
     1981 gagattaata aaaactttag ccttaacgct cttgtcacag aaaatcactt cgaagcacct
     2041 aaggatgtaa aagaagttct cgacgagatt aaagaagaga aaaaggaaga agaggagaaa
     2101 aagtcttctt ggtttagtac aataaagaaa gtgtactata atgaaactct tcgcggtgtt
     2161 ttcaaaattg gcaatacact ttatacacgc aaggacgagc tcgacaaaag aaaattgatt
     2221 cttcagactg caaaggaagc ttataaaaat cttcccctgt ggagaaaaat cactggatgc
     2281 actggaaatg tgggaacaga tcttgatgat gcacaagaaa aacatgacga gttaaaagac
     2341 tccgaaggta tcaaaaaatt tgataaaaaa actgagaaaa attttgatct tacccttgga
     2401 aagcttgatc tctttgtaaa tcttggcact tttggatata acgtttatta cgcatatcaa
     2461 agcggtagct ataaaaatct aagtgattat gttctcaccg gtattgaaga aactacttac
     2521 tttgataact acattgagaa taagattgaa aacgctcttc gtggtgttgt aggaactgta
     2581 gcaacagcgt actttggtcc tgctttttcc tggtatgtta ctgaatttgc aacgattaca
     2641 ctagagaaaa caggcgcaac aaagacaatt acaagcaatg gcgcttgggc tattaaaaaa
     2701 agtgtcggac ttggattaaa gggcgcaaac tacctttaca actactacta tactacaaac
     2761 gatagcacta caaacgatag caaagagctc gttccagtag tagcagctgt ctaaacgtta
     2821 aaacataatt tttcttgaac tgacccagct tcggctgggt ttttttatgg aaaaaagatt
     2881 aaaaacactc taaaaacctt aagtagtctc tgtgatcccc tggcaaatca ggtgattgtg
     2941 aaagcattaa aggaatcgta aaattttaat aaaaataata ataaaatttt atcaaataaa
     3001 tctttcaaat atatgcattt ttctcttgaa tattcattca atactgctta gataaagtgt
     3061 atcgaaaact ctaaccaagg atttagacat gttaacaaaa aaagttacgg ttctttctgt
     3121 cgctctgatg ctctcggcaa cagtagcacc ttctcaagca ggcctctggg atgtgtatga
     3181 ttatgcaaaa aatacattca acagcgccaa aaaaactatt aacaaaatcg acggcatcat
     3241 agacaatgct actcaaaacc taaaaaacaa tataaaaaag gctgtggatt tttcccttga
     3301 ccctaataga ggcgataaaa tcattagggc gattggtgac tacacaggta agcaaaatat
     3361 tgctgagact atcatcgaca tatctaataa aattgatagt ggcatcaagg ttgttgaaga
     3421 agtagtcggc aatgtaatca aaagtgagat taaaaatatt attaatacaa cttatgacga
     3481 tatcaacact gcgtacacaa ataccaaaga attctatggt ctagttaact acttctacta
     3541 cgttttctaa aaaagacgta gcagctgcct aaacgttaaa acataatttt tattgaactg
     3601 acccagcttc ggctgggttt ttttatagaa tttttacctg cacaaacaat ccctggtgat
     3661 ttaattttta attgattaaa atacattcta aatatcacga aaaaagatac tcctatgtcc
     3721 gcacgcgcca cctctcctca caatgatctc cacgagagct atttgaaatc tatgcgtgag
     3781 atccctcttc tcagcagtga ggaagagtat aagcttgcca aagcctggcg tgatgaaggc
     3841 aataaggaag ctgtcgccat ccttgtgaca agccacctcc gccttgtgaa taaaatagcg
     3901 ggcggctacc gcggttatgg cttgccctta agcgatttag ttgcagaagg ccacgttggc
     3961 gtgatgcaag ccgttaaaaa ttttgatcct gaccgtgggt ttcgcttttc gacctatgcg
     4021 atgtggtgga ttaaagccag tattcaagaa tatattctca actcatggtc tcttgtaaaa
     4081 ctaggcacga cctcgtcaca gaaaaaactt ttttttaaat tgcgttccct taaacaacaa
     4141 ctgggtgttg cgcagctttc tcccgaacaa gtgcgcacca ttgccacaga tttagaagtg
     4201 aaagaatcgg atgtcatgat gatggaggaa cggatgagcg gcggagatcg ctcactcaat
     4261 gctgtggtgg ggaaagatag cagcgacagt gatgttgagt ggatggattg gctgacggac
     4321 gatcgcacaa atcaagagca tgaactgttg caccgcgatg atttagagca aaaacaaaaa
     4381 atgctgaatg cagcgctcaa ggcccttcac ccgcgcgaac gccatatttt ttacgataga
     4441 cgccttgttg aaccgcctca cacccttgat gagctcagtc acaaatacca tatctcccgc
     4501 gaacgcgttc gtcagattga aatgaaggcc tttcaaaaac ttcaaaaggc catcaagttt
     4561 catgtctttg gaaaaatgtc cccttagact atttgcgtat cgttattgtt cgatgtactt
     4621 gttgtattgg aaacaacggt ttcttgctta ttaaagagcc ctgtgagata agcctcagca
     4681 tcctcagctt tactcaggcc ttcattgata tacccaaaaa ctgatttgag agcattttgc
     4741 tccttctctg ttcctttagc tatctttaga aaaagcggac catatttttc gataaaactg
     4801 ataacaattt cactatcttt aacaatagtt tcactatctt taagaaccgt ttctccttct
     4861 ccaacgactt tttgtatacc ccccccttta ataaaactca tgattgtttc aactttaccc
     4921 tcaagaaact taaaatcctt tacaaggtca tttttaacag taattgcttc attttctgct
     4981 ttcttaccca gatctttcag cttattttcc acaagaatcg catcttcttt aattttattc
     5041 tcaaactttt gaagcgttgt tatttgctta ttttgtgttt ctgtgagttt tttgagctgg
     5101 tcattatgtg ttgcaattgt gttttgtgtt ttggaaaaat cgtttttgag ctctttaata
     5161 acgttttcct caagctcttt agctttattt tcaagatttt tagccatatc ctcaaagagt
     5221 tttattttat ttagaacacc gccctcgact gttttaattt cctcctcaac aacagtcaac
     5281 aaagaaacat gctgtccatg agggaaaatt ttctcaattt cttgtttgaa tttcttttcc
     5341 acgtcttttt cgagtttcac tactttttta acgtcgtctc ttactttatc ttcaaacttt
     5401 tgaactaatg ttttcacagg ttttttttca ttattttcta aaggggtttc gtccttcaga
     5461 tttttactac tcgcacaaag gggggagaga aaagatgttg tgagcgcgaa tgaggaaagg
     5521 actgtcataa gaaattttaa tggcataatg gggatctcct tttgggtttc agataacgga
     5581 ttaagtcccg tcacatatca aggagtaccc cacaaaaatt aacaaaaagt taaaatgagc
     5641 atatttaaaa taataaaaat tcacaaaaat ttataataaa aattcaaaaa atggacgttt
     5701 ttcaatgtat gtcaagagat aaaggcttta aacgcctcta tctctcatct atttttttat
     5761 ggttttttct ccatttctgc atacattttc caagtaatat cttcataata atgaagtgtg
     5821 tcctcaaagg tctccactcc aggtctcttg ctgcgagtga ccatttgctt tgccgtcgtt
     5881 gtttgaggcg ttccgtgttc gtctaaaggc aatgaaagtt tttgatgtct cacttttttt
     5941 ttcactttca aaacaaggtg aaagtttctg atgtggtttt ttactcaaag cccctaagag
     6001 ttcatcgtta tcaactgcaa caacaataga tgaatttgat attagaatgc ttaacgctcc
     6061 ataccctata gctgctataa gtctcatcgt atgcctcctg ttctggcgag gatggtaaga
     6121 caccaccgta aggggaagta ggtcaaaaaa aatttaaaac aagattaacc taaagaaata
     6181 gtttttaaaa aaaatctcga gaattttagt cctttattaa tttttttggt atagtctcta
     6241 gaggtgagct tttgcgactg tctggataga tttttataaa aggatgctct gtttgtgaac
     6301 acatcactca tcgatattct ggaggaattt ttaaaactta acgcctcgcg tgacgcctcc
     6361 tttattgcca ttaaaagcct ggcagagggc cttctcattg ggtataaatc gaccataggg
     6421 atgaaggatg cttttgaaac agggtgtgac aagctcgagg aacttaaaac aaatttcctt
     6481 gatgaactgc gcaaaagcct ccccgagttg cctgatcttt ttgctcaaga aaacacccaa
     6541 gatcttgttc acctccttga aaaactgaac atacgggact atttgtttga atccaccctc
     6601 aaaagtcaaa tcgagcgctc acaaaaacta gaaaaaatag aacgtttatt aaacctttct
     6661 gctgactata cggaaatacc gactttttca aaggctcacc ttctgattat cgagccaaag
     6721 tcctcaaaaa tcgcctccac tctctcgagc gaacactaca ccctctacga agcctccact
     6781 gctcaaaagg ctgcctcttt tctcaaaaaa aatccgatcg atattttgct tatacaccac
     6841 acatgtttag agatgctcac aggtgaaatt cgcgattatt gtctcaattt tacctccaac
     6901 attatgatta ttgtgattgg aaaaggggat gatttctcac tgattaatca ctgtttttct
     6961 cttggcgttg atgattttat cgcgcttccc cttcacgaaa cgattctcca ccatcgtatt
     7021 gatgcgcttt ttgaaaaaag ccaactgaac cgcctcaatc ttaaaaaaac acgggatttt
     7081 aaggattcaa tggaggaatt atcccgtatt attgaggata ttgaggatgt ttttattctt
     7141 ttaaacgatc aaaaccgcat tattagttat aacaagcgta tttttaatat ttttccacac
     7201 cttgagcgtg attataaccc aggatcctct gaatctcttg tgggcgaaaa ttttatcacg
     7261 cttattcaga aaaatttcaa acggcatatt tatgatgatc tcttgcaaaa aaattctaaa
     7321 acacatttga aaaatttaga ggaaaaactt gcagagcctt catgcgagtg gcaggaaaat
     7381 ctttgccagg gtgctgccct ctcttaccgc ctccaggcca ccattgatgg caataaaatt
     7441 cttctcatta aggatcaaaa cgcaaccaat ctgggccgtc atcacctggc ttatcttgcc
     7501 tatttcgaca gcttaacagg tacaatgaat cgtcaatttt tcatgcaacg ccttgaacag
     7561 atgatgtctg atcaaaataa tcgcatcccc cttgtgcttt ttatgattga tcttgatggc
     7621 tttaaagagg tcaatgacac ttatgggcat gaaatggggg attgggttct ccaacaactg
     7681 gcacatcgcc tcaaggcatc tgtgcgccag ggggatattg tcgcacgctt tggcggcgat
     7741 gaattcacga ttgtccttaa aaatattgcg agtgaagaaa ccgtgcgaga cattgccaca
     7801 cgcatgattg aaacgattac aaatgtttac gttcggggca atataaaaat atccatcggg
     7861 gcaagcattg ggatctcgcg ctaccccctt gaaagcacca cgccaaagga ccttgtgggg
     7921 cacgctgaca ctgcgatgta tagcgtaaag cagcatggca aaaataattt tcgcttttat
     7981 cacgagcttg tccttccgaa aaatagcatc gtgcattaaa ggttaattca actgaggaca
     8041 ttcgttttta atcaattatg aattttctga acgcagctct tgttgctcaa gacgacgcag
     8101 ctgatcgcgg agtttcgctg ctttttcaaa attaagatct gctgctgcct cgagcatttc
     8161 tttttcaact ttacgtttat caagggcgtt tttctttgtt cctgaatcta tttcctgata
     8221 aggagccttt ttctcacgca gttgacggat cacagtttga ggcgtgaggc catgagcttt
     8281 gttatatatt tcttgttttt cacggcggcg attcgtttct gctaaagccc cctcaatgga
     8341 tttcgtcatc acatccgcat aaagaatcac cttgccctcg ctgttgcgcg ctgcacgacc
     8401 aatagtttgc aagagcgatg ttttcgagcg caaataccct tccttatccg catcaagaat
     8461 cgccacaaga gcgcattcgg gaatatcaag tccctcacgc agaaggttaa tgcccaccaa
     8521 aatatcaaaa tcacctgcac gcagctgttg cagaatgtca atgcgctcga gggtctcaat
     8581 atccgagtgc atatatttta cttttaagcc agcctcactt aaataatcgg tcaaagcctc
     8641 tgccattttc tttgtcagcg ttgtcacaag cacacgaaaa cctttttgaa tcacctcttt
     8701 gcactccgcc ataagatcat caatttgatg ttggcagggt ttaacaaaac actttggatc
     8761 caaaaggcct gtgggacgaa taatttgatc ggcaaattca ccctcacaaa gcgtcatttc
     8821 atacgcccct ggtgtagctg aaacaaaaat cgtttgaggc ctgaaatgtt cccactcttc
     8881 aaacataaga ggcctattat ccgcacagga cggaaggcga aatccgtact caaaaagcgt
     8941 tgttttgcgc gctttgtctc ctttgtacat ccccccaatt tgcggcacac tgacgtggct
     9001 ttcgtccaca atcaaaagcg catctttggg aatatattca aaaagagttg gcggcggctc
     9061 gccgggagca cgcccggtca ggtaccgtga ataattttca ataccattgc acgtccccgt
     9121 cgcgcgcagc atctcaagat caaaacgcgt gcgctcctta aggcgctgat gctcgatcag
     9181 tttcccctgc tcttcaaatt ctttgaggcg cacagtaaga tcatgctcga tcattttaat
     9241 cgcctgggcc atcgtcggcc cacctgtaac gtagtgactg ttgggaaaaa ttttaatcgc
     9301 actgagtttt ttaaattttt cgcctgtaag cggatcaaac tcttgaatgc tctcaatatc
     9361 attatcaaaa aatgttacac gccacgccat ctctgcatag tgggaaggga aaatatccaa
     9421 cgtttctccg cgaaggcgaa acgtgccacg gctcagcacc atatcattgc gtttatactg
     9481 gagctccaca atcgagcgga tgagttttgt gcgatcgacc acccctccca ccttaaggtc
     9541 aacagccata gagctatatt ctgcagcact tccaataccg taaatacagg acacactggc
     9601 cacaataatg acatccttgc gctcaaaaag agcgcgcgtg gcactatgcc gcatgcggtc
     9661 aatttcctga ttaatcgacg ctgttttctc aataaatgtc cctgagcgcg gcacataggc
     9721 ttcgggttga tagtaatcat aataggaaac aaaatactcc acagcattat ggggaaaaaa
     9781 acccaccat
//
LOCUS       DAIAHG010000024         9762 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_24_length_9762_cov_2.902943, whole genome shotgun sequence.
ACCESSION   DAIAHG010000024 DAIAHG010000000
VERSION     DAIAHG010000024.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9762)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24_length_9762_cov_2.902943"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2774 a         2088 c         2128 g         2772 t
ORIGIN      
        1 atcacaagac tatagcatag atcagacaaa gcgtttttaa tctctccctc tttgaacata
       61 tcaaggcaca ttgccgcttt ttcagcatag gcccaggcaa ggtcataaca ctctttaatc
      121 gcatcggttt tatgcataag ttctataatg tgcgcaaaat catcctctgt tattttttca
      181 ctctcaagca tttgttcgag cattttttga tcctccgcac tacacttttg ataggtgaaa
      241 aaaagcggca acgtgacttt gccttccatc acatcgtcgc caatattttt ccccaaagac
      301 tctgtgtcgc ctgtatagtc aagaacgtca tcgatgatct gaaaagcaat gcctaagtga
      361 tgaccaaaat gatgcaacgc ttgctgaatc tctgttgtcg tatttaaaag agcggcacca
      421 atttcacagg aagctgcaaa aagagccgct gtctttgttt caataatgcg catgagaata
      481 gaaaaatcga gggcgaatgt cttgaggtag gagagttgaa aaacctctcc ctctgtaatt
      541 atttgcgcta cggtggcaag gataggcagc acgcgttttt cattttctcc aagcattagc
      601 tcaaacgctt ttgcaaataa aaagtcaccc acaagaatgc tgggcttatt cccccacaaa
      661 gcattggctg tgggacgatt gcgccttaaa ggtgattcat caataacatc atcatgcaaa
      721 agcgtcgcat tgtgaataaa ttcaatgctc gctgcgagct cgaccatttt tgagggcgtt
      781 tcattcccct tttgctccgc cgtgaggagc ctatagcttg agaaggctaa aagtggtctt
      841 agacgttttc cccccgcaag aataagatgt tttgcaatcg tactaatcag gggcacatca
      901 cgctcaatat gcgtcaaaat cacaccattc agcgcctcta aatcattaca gcaaagggat
      961 tgaagggttt caatcacgtc ggctgttttt ttctgaggta cagatgaaaa agaatttttg
     1021 agagaaaatg ttgacgcgac cataaatatt gcgattgagg attctttaag attcaatcta
     1081 ccgctaaaag cgtctaagct atagtacaaa atgacaaaag atgtaaaaag ttagaatgtc
     1141 tgtgaccgaa gatacattta cgcaagatta tctcttgaat ggaagaatca caattcgcca
     1201 gcctgtgaat ggctaccgtg tggcgattga tcctattttc ttggcggcgg ctgtggatgc
     1261 aaaagaaggc gatacgatcc ttgatattgg tgctggtgta ggggctgcgg cattatgttt
     1321 agcgcttagg cagcctcact gtaaaatcac aggccttgag attcagcgcg attatgtacg
     1381 cttggcctct caaaatattg atctgaatca cctcaaagga cgcgtcgaga tgcttaatgg
     1441 cgatttactc cgtccaccac cacgccttgc agcgggtact ttttcccatg tgatgaccaa
     1501 tcctccttac cttgaaggac acaaaggcag aagttctccc aatgcggacc gaaacattgc
     1561 aaatcaggaa gacgaagtct ctcttgaggc gtgggcacgc ttttcacttc ttatggtgaa
     1621 acccaaggga accgtgacat ttattcacag agccgaccgc ttggatcaca ttatggcgta
     1681 tttttctggc aagcttggca atatggtgat ttatcccctt tggccgggca aagataaacc
     1741 tgcaaagcgt attcttgtca gtggtgttaa aaatatgagc ggccccctta aactttcaca
     1801 agggatgtac cttcacgagg cggatggcaa atttacacgg gccgcagaat ctattcttcg
     1861 ggatggcgaa cgtttagaac tttgacaata aatagaggca tacgatagag gcataactcg
     1921 cgtcactaaa atacgtgtga tttttatcca accttaagag tttattcatt tttttgagct
     1981 agctttagga gatggataat tttcatcaaa ggtttccttg aatgtcaaca ccctctcttt
     2041 atgatcaact tggcggcaca gaggcgatta atgcggctgt tgaccttttt tatagcaaaa
     2101 ttttagatga tcaacgcgtc aatcattttt ttaacgatgt tgatatgaag gtgcaacggg
     2161 cgaaacaaaa gggtttcctc aaagtagcct ttggcggccc ccatcactac actggcaaaa
     2221 atatgcgcga cgctcatcat catcttcttg ccaggggcct taatgatacg cactttgata
     2281 ttatcaaaga gcacttacaa tccacgcttc aggatttaaa tgtcaatccc cacttaattc
     2341 aacaggtgag tgcgattgcg gaaagtgtgc gcagcgacgt cttagggcgc gcgtgaggat
     2401 agcctaactg cctcatcggc ttgttgtttg agagcggctt ttcgttgacg ctttttccgc
     2461 cgaatatatt gcaacatcca aaggggtgga aagacagagg tcgcaacaag gcgcgtcgtc
     2521 cccccttgta ataacatatg gataaagatg agcgcttcgg caatatagac tttattatga
     2581 atacgtttcc aaagagggaa tcccagtttc ttaagcgata ctttattgct tgtgatggcc
     2641 agaatggaaa aaataatcag cgccagaaat gctggtacaa taatcggatg aaaaagccat
     2701 tgtgtaaaaa aagaccaccc ttttttttga atatataaaa acgcatggca aaaaccgtac
     2761 gtaaaaacag ccaccccaat gatgcggcgg tgatgattaa gcttttttaa aaaaacacgt
     2821 gtgggaaaaa gtgaaataag cggatttaac gaaagcacaa ttataagcaa tgcaagggca
     2881 cagtacccta aagtcgacag catcaatcca cgcgcatcag gcattaaaaa cgcaaaaata
     2941 aaaagaaaaa tagcgggaag attaagaaaa atccaatctt tgtgtttctt gaacatcagc
     3001 aatcaatcca cataaatgca ttggtgtcta tatgcccccg cagggtctat atttcaagaa
     3061 aaatacttgt tttacccctc tatttttttt agtgtgggga aaataattct tttaaaaatt
     3121 gctcacatgc gtatttctct caagcggttg ccccatgcca cggatcttga tgtgcctttt
     3181 tatgccacag aaaaaagtgc gggtatggat ttatccgccg ccattcacga gcctgttgtc
     3241 cttgcaccgg gtgaacgtgc gcttattccc acagggtttg cctatgcttt tccagaaggc
     3301 tatgaggggc aaattcgacc acgttcgggc ctcgcgctga agaatggtct caccgtttta
     3361 aacacacccg gaaccatcga tgccgactac cgtggcgaag tgaaggtcat tttagtgaat
     3421 cttggcacag agacttttac ggttgaacgc ggaatgcgga ttgcgcaatt tattatcgcc
     3481 cccgttcaac aagccgtgct cgaagaggtt gtcaatttgc cagaaagtac gcgcgatgct
     3541 tttggctttg gatccacagg cctttcacag aaaaatgcgt aaatgttgat tgtcaggaac
     3601 ggaagaggta gagttaaaat gttctacctt ttttcgagct tgtttatttt tcaatgtttg
     3661 tcctcctgct tttgatattt tctctcgtga aaagccccgc tttagcggaa cagcttgcta
     3721 aaaaggagag tgccaaagat acaatcgaga aaaaaacttt ggaaaaaaaa gagaaatcat
     3781 tcaaaacttc tgcgaaagct gagaataaaa aaaatcctgg gggtgagcct aaaaaactcg
     3841 acaaaaagaa atctcaaact tctgaaaaaa cactccctca ggccaaaatg aaaaatctta
     3901 aaggggagga taaaaaagat gaaaaaagta tagatcaaaa agcgagtgtt gccaaaaaag
     3961 gcgaagctcc tcccaaagca gcagatccgg cgtcgctcaa aaatcagcct caaaagcttc
     4021 ctctgccacg gttcgtctgt ataaaagcca gcagtgcgaa tttgcatgtt ggccccgggg
     4081 atcaatatcc tgtggagtgg cgctatgtta gagcattcct gcctgtggag atcattgcag
     4141 agtttgatca ttggcggcaa attcgagaca gtcaggggac gacgggatgg ctgcataaaa
     4201 ctcttttgtc gagcaaacgc tttgccatga ttcaaaataa agttgttaat ttctatgcgg
     4261 attcaaactt agagagtcgt gtcattgcaa cgcttgaacc tggcgttgtg ggcaaagttt
     4321 tagaatgtaa tacacttttt tgtaaacttg atgttcaagg gattcgtggg tggattaaaa
     4381 gggattttct ctttggggtc tatcccgatg agcatcgttt ttagcggctc ctttttttat
     4441 gattattctt ttaaatgaga ttcagcttgc cgaaaattta ggagccattg cacgggtgat
     4501 ggggaatttc agcatgaatg agcttcgcct tatttctcca cgggtatcac cttcggatcc
     4561 aaaggccctt gccacctcta cgggagcaga gtttatttta aaatctgctc aaacctacga
     4621 ttcagttttt gaggcgacaa aggatattga gtttcttttt ggcacatgcg gcgatgatca
     4681 tcgccaaggc attcgtcatt attttttacc gcatacggct tttgagaata gtaccagtga
     4741 gcaaaaaata ggcgtgcttt ttgggtgcga gcgcacaggc ctcagtcaag aggatttaag
     4801 ttattgccgc gcgacgattc gtattcctac atctctgcat ttttcctcga tgaatgtcag
     4861 tcatgctgtc gccattgtgg cctatgagtg gtgtcaaaaa atccaccaga ccgaggggca
     4921 tcagatgcat tttggaaaaa caaagccggc cacacagcag gaaaaaaaag cgctttttga
     4981 ttaccttgtg agaaatttgg atgctgtgga ttattggcgc gtggcctcta aaaaacctct
     5041 catgacggaa aacttagcca atcttttttt tcgcatggac ctcacggcgc aagaaataca
     5101 aacgcttttt ggggtatttg atgcacttaa gcactaattt tttttgttta ttattgaatt
     5161 tctattggct atataaaatt gttcaacatt aacttatgga tatcatgatg attaaagcac
     5221 ttttcacact tactcttttg acaacgacgc tcgcttatgg aagtgagcaa gagaaaattc
     5281 cttttgaaga taaagaactc tccgtcaaag aacaaaaaga aattattcaa aaaaacatta
     5341 atgaccttac gctagagaga aaaaaagtta gtgaaagaag agatggctta gtcaaccccg
     5401 tttctctgtg gaatgaacgt atagatcttt ctttaggatt aacgaataat acagacaaat
     5461 tacaaagaga ttggaatctt cagcttgtgg atctatctca aaaaattgaa gaactaacga
     5521 aaaatttaga atctttcggt gatgatgcgt aaacagtatc aaagtaaatt ttagacaatt
     5581 cggggctgtc tctttatact acacagcagg atagcccttt aaaaaattat acgatgcctg
     5641 ttgtacttat tattcttgat ggttttgggc tgagcccaga tcaaacattt aatggtattg
     5701 cacaggcgcg cacaccaatg cttgattatc tgctggcaac ctatccccac agtttattag
     5761 aagcctcaga agaatacgtt ggccttccca aaggacagat gggtaactcg gaagtggggc
     5821 atttaacttt gggagcaggg cgcattgtca ggcaggggct aagtgcgatt gatcacgcga
     5881 tttccgtgag cgattttcaa aaaaatccac agcttttaac gtttatcaaa aactcaaaaa
     5941 caaagcgatg ccatcttatg gggctttttt ccgatggcgg tgtgcatgcg catatgcatc
     6001 attttaatga agcggcaaag attttaatcg atcagggcat cactgttttt atgcatcttt
     6061 tcaccgatgg gcgcgacaca ccccccaaag atgcgctgag gtttatcgat cttttgccaa
     6121 agcatccgct tcttgtgcca gcaacacttc aggggcgttt ttatgcgatg gatcgcgatc
     6181 atcgttggga ccgaacagag cttgcttacc gtgcaattat taaaggcgag gggaagcgtg
     6241 ttgcgtcttt ccaagaggcg attcaacaaa gttatgatga taatataacc gatgaattta
     6301 tcaaaccgtg tgtcatgggg gattatcaag gggtgttgcc aggagatagt cttttccaca
     6361 cgcactttag agcggatcgt gtgaggcaac tgctgggcgc tcttgtattg cctggtttta
     6421 gtgatttttc ccgtgagctt gtttcttggt cggccacact tgggatgagt tcctacagta
     6481 atgccttaga tgcttttgtg cctgcacttt ataaagcaga gcccgtacgt aatggctttg
     6541 tgcaaattct ttcacagcat cataaaaaaa tattaaaaat cgcagagaca gaaaaatatg
     6601 cgcatgtgac ttttttcttt aatggcggta tagaagagcc tgttgcaggc gaagatcgca
     6661 ttcttgtgcc atcgccaaaa gttgcaacgt atgatcttgc gcctgaaatg agcgcacggg
     6721 atatcacaga aaaattatgc gcggcgattg cgtctaaaac gtatgatgcg attattgtta
     6781 attttgcaaa tcccgatatg gtgggacaca caggcgtcca ggaggctatt cttcaggcga
     6841 ttgagtgcat tgacacctgt cttgagacta tttataagca catatgcaac gctgattaca
     6901 ccttgatgat cacagcggat catggtaatg ctgagcaaat ggtcgaggat cataatcctg
     6961 acaaaccgca cacggcgcac acgtgcaatc ctgtgccctg ccttctcgtg aatagttcct
     7021 atcgtgcgat caaaaatgga acgctggcgg atgttgcgcc cactctttta aagatcatgg
     7081 agattcctct tccagacaca atgacgggcg tccctttagt caaataataa agacagcgtt
     7141 agaaaatatt aatccccaaa acaaatgcca aggccccgcg caatttgcat catcgtactt
     7201 tcactatcca caagcttttg agaggctgtg gcgatttgga tgggaacggc aataatatct
     7261 tggcctttga gcgcaaccat gtgattaaac tgtccttttg caataagatc caccgcactg
     7321 acaccaaact tagacgcaag gatgcgatct tctgcacaag gctcgccacc gcgttgaaca
     7381 tgacccagca cggtcacgcg ggtttctgcg cctgtggaaa gactaatttt ctcggataga
     7441 tagtgaccaa tgccgccata acgcagccgg cccgacttag gatccgtcac agtcaacggc
     7501 tgaccatctt cttttttcac accttctgcc acaacaataa gcgcaaagtt tgaaccatcc
     7561 atcgccagtg ttttaatgcg gctgcacact ttatcgaggc tatagcgaat ctctggaatt
     7621 aaaatcacat ctgcacctcc cgcaacacct gctgcaaggg caatgtggcc cgcatcacgc
     7681 cccatcactt ctaaaatcat agcccgctga tgactggcgg ctgtgggttg aaggcgatcg
     7741 agggcctctg tggcgacctc caccgctgtc aagtgaccga ttgcattttc agtgccggca
     7801 acgtcattat caatagtttt aggaattgtt acaagattga gaccgccgag atcacacaga
     7861 cgcttgagaa ttttcatgct gccatcccca ccgattgcaa tcagcgcatc aagcccaagg
     7921 tcgtgataat ttttgataaa atccttagag cgatctttaa ctccgccatt ttctgttgga
     7981 aaataaaaag gattatcctt gtttgtactg cccaagaaag taccacccgt acgcataata
     8041 gcggtattgc acagcgaggg cgttaactga atcgtatcaa gaggactccg caaaaggccc
     8101 gttgtgcctt tttttatccc aagaacttcc catccataag tgccaacagc cttgtagacg
     8161 atcgcacgta ttgttgcatt gagtcctggg caatcccctc cacttgtcat aacgccaata
     8221 cgttttgtca tctgcttaac cttgtgtttt tatttatttt tcttaagagc tatcttagtg
     8281 aaaaaaaaca catttgttaa gcgatggaat ctttttttat ttattttttt gtgattctgt
     8341 cagcacttta gttttcaccc ttattgttca aactttcctt aagggaaaaa cttatcaagg
     8401 ccacccgata gttttttaaa caatgttttg tcagtctcat acctttcctc cagtgtggaa
     8461 gaaagttccg tataaaaaac atcgtcaagt gattctttgt cttcatcttt aaaaaagaga
     8521 tgaccatcaa tgcttttgat aagatatcca cctttatatt cttcgatatc ccagtggtct
     8581 ttcatcagtt ttaaaaatgt atccaaacat tttttcatca cgcctgcgga accatcctca
     8641 aagagaacca cctgcccaaa gggtgtccac agtaagcaca ttggattttt tgtaagaggg
     8701 atggactcta aaaataaata tttttttgtg cgaaacgaca aagggtttgg tgttgagaaa
     8761 agagcaaaac tctttatgac gtacactttt tcgtttttta gcgcatcttc agtgacttca
     8821 ttgatggctt tatcaagctt aagacggata aattgaatgc ctttgaagta ctcagacatt
     8881 tcttgctcat catcccacac gcaatctccg ccgtgactgg tttgataaag cgctatgaaa
     8941 aaagcaagac cgattgaaac ggagcgtcga ttaaatatct tcatgaggtc tctcccttgg
     9001 tgcagacaca ggaaagcaag ctgctctctt aacgtgattc tactgtagaa aatttaacaa
     9061 agtgtaaatt ttttgagagc gcacctccgg gagttgaaaa tatgaggtgt tttttaacca
     9121 cagactagct tttctatgca taaaagcgta taaatgatag caaagtaatt tttagtatga
     9181 gtaatcttat ggtgattctc cttgttcttc aggcgctgat tgcgctggct ctgattagtg
     9241 tgattctttt gcaaaaaagc gagggaggtg gccttggtct gggcggcggc ggtggtgctt
     9301 cgaataatgt gtttacggca cgcggggctg caaactttct gacacgttta acagcgatcc
     9361 ttgcgggcgt tttcatgttt aattgtcttt tgatgagcat tattgcaagc cgtcagattc
     9421 ataaacaatc ctcttttctc gatactgaaa gcactcaaca agaagcccct gctcaaccta
     9481 caacggataa ataataaaaa ttgtgacacg ttatatcttt atcacaggcg gcgttgtttc
     9541 ctctcttggc aagggaatcg ccgcctcttc tcttggggct cttcttcagg cccatggtta
     9601 ttcggtgcgt attcgcaaat ttgatatcta ccttaatgtt gatccaggaa ccatgagtcc
     9661 ttatcaacat ggtgaagtct ttgtgaccga agacggtgcc gaaacggatc tggatttggg
     9721 tcattatgaa cgttttgtcc gtgcccgtat gtctaagtgt aa
//
LOCUS       DAIAHG010000025         9700 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_25_length_9700_cov_3.452042, whole genome shotgun sequence.
ACCESSION   DAIAHG010000025 DAIAHG010000000
VERSION     DAIAHG010000025.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25_length_9700_cov_3.452042"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2801 a         2181 c         1887 g         2831 t
ORIGIN      
        1 aaggcgtact tctcatgcga aacaactcaa atatgcacta ctgcgatcaa aaaaggccca
       61 taaaagcgaa aaaatcgggg gaaaacagga aaaattagaa ttttctaaac atttatacta
      121 gtataaaatg tttcaacttt cctcatagcg ttaaacatca gaagagaagt ttttgttaaa
      181 aaaagtcaat gaatggtcag ttttagtcct tttataaaga tgttggcata aaaattgcat
      241 ctattttgat taaatctaag gaaacaatac aatgtatatg ctttatcacc ttcatgaact
      301 taaaaatgcc gcgctttggc ctacacacat ggcgacagaa gctgcatcgg cccttttgga
      361 cgctgttgat gccgatcatt caggtcttta tggtcgttat atgcgagcat cgacagagct
      421 tttagagcgc aatacccgta tttatgaaaa gcctgccttt ggtattacac atgttcaacg
      481 caataaagcc gttttagccg ttgaagaaaa agagcatttt cataaaacat tttgcacaat
      541 taaagagttt tcacgcacag cgacaacgga ctctcttaac cgtgagctta agaatgaccc
      601 gattgttctg atttcagccc ctctgtccgg acattttgcg actcttttga gggatacagc
      661 gcgcgcagtc attccttttc accaaaccta tattacagat tggaacgact caaaacttat
      721 ccctgcaagc aatggccctt ttaccctaga agattatgtg gaatacaccc tcgattttat
      781 tcgaagcctc actcaaaaac atgcaggacg acgcattcat attattgctg tgtgccaacc
      841 ttccgtgcct gtactgatgg cgacgagcct gcttgctctt tataaggaaa attgccaacc
      901 ctactctatc acactgatgg ggggacctat tgatgcccgt atcaatcctg gaaaggttaa
      961 tactttttca acagaacata gcctcgattg gtttaagaaa aatattattt cccccgttcc
     1021 ctcttattat aaaggggccg gacgcgatgt ttgtcctgga tttattttgt tgggtgggtt
     1081 tatgagcctc aatcctgagc gtcatcaaga agcgatgaat aaactgtttc agcatcttgt
     1141 ccaaggcgat atggagggtg tggatatgca ccgtaaattt tatgacgaat accgtgcagt
     1201 gatggatgta cccggtgagt atttcttaga tagtatttat cactcctttc aaaattatac
     1261 attggcaaaa ggagaaatga cttggaaaga ttaccccatc acgcccgagg cgattaataa
     1321 aacagccctg atgacggtgg agggagaact cgatgatatc tcttgcccag ggcaaacact
     1381 cgcagcccat aatttgtgca aaaatatccc tgaacataaa cgcaaaactc ttcttcaaaa
     1441 gggcgtaggg cactatggta tttttaacgg aagccgttgg cgcaatacga ttcaaccgag
     1501 cattgccgca tttattcgcg aacaggagca ataaataaag tattcgctta cataatattt
     1561 ttatttcgcg tattctgctt ttacattaaa aacagagctt tcctcaacgt atgagatccc
     1621 ttggccttta tattcactgg cccttttgcc tctccaagtg cccctactgt gacttcaata
     1681 gttttgtcag caacactatt gatatcgaag agtatacaca ggcctatatg catgagcttg
     1741 agcgtttcta tgcagaaacg tcagcgcaca ttcttcaaac tatttttttt ggcggcggca
     1801 caccctcgct tatgcctcct tccctcactg ctcaacttat tgaaaaagca caaactcttt
     1861 ggtgccacaa tgattctctt gagataacgc ttgaagcaaa cccaagcacg gcagaaacga
     1921 gtcgcttcca agcctttaaa gacgcaggga ttaatcgact ttctattggc attcaatctt
     1981 tttctgataa tgatttgcat tttcttgggc gaggtcattc agccaatgag ggcatgaaag
     2041 ccattgatat ggcgcaaaag atttttaatc gtgtgagttt tgatctgatt tacgcgcgtc
     2101 cctcccaaac attgtctgat tgggaaaagg aattgcgcca aggtcttgct tttggcacaa
     2161 cacatctgtc tctttatcag ctcacaattg agccaggcac agcctttgcg ccacgttatg
     2221 accgtggaga aattatcttg ccagaagacg atcttgcagc ggacatgtac aatctcaccc
     2281 aagctattac tgctgaaaaa ggtttgccca tgtatgagat ttcaaatcac gccatgcctg
     2341 gatttgaaag tcagcataat ctgatttact ggcgctatga agactatgtc ggcgtggggc
     2401 caggggctca cggacgctat tataaccaca ggtgggaaaa aattgccgca gagcaacaca
     2461 aagcacccaa tgtttggtgt aaaaaagttc tcaatgacgg ctctggcacg attcggtcca
     2521 ccgttattac agaaaatgaa caactgtgcg agcgtttaat gatggggctg cgcttaaaag
     2581 aaggtttgtc gctaccggat gtttcttttg taaacgagaa agccctccat accttaatta
     2641 catccggtga tttggtgatg gagggagagc atatgcgcct gacttctcaa ggatttccgc
     2701 gccttaacgg cattttgcgg tacctcttga gaaggtaaat gctccttagt ttttatcaat
     2761 aatttgaaaa atcgaagcct ctttgacttt ttgtttcttc tgcataggtt tttcctcgtc
     2821 agaggggtct tcttctttcg cctctcccga gtctatatta ttaattttat cttgctcttc
     2881 tcttatcact aaatttttat ttcccttata aaaagtgctt ctggaatctt tctttaaaag
     2941 ctccaaacat ttttgcaaac tagcttgatt tctcgcttga ggcgtattgt ttttaagctt
     3001 agacagctcc tctgttgtga gaaaatcttt gggaagtttt ttaaggccat tcaatatcaa
     3061 atactctttt tttgcttttt ggattttttc acgatacttt gcctgaggat cgatgcaact
     3121 tttttcttta gagtgatttc cagtccttac gtattttctt ttatttggat taatattctc
     3181 tttgcttata ttttcattag aaccatggct tacactgttg aaaacaaata acaacgccat
     3241 gagataaaaa aatttactta aattcattac actttctcat ttttatactt aatattaact
     3301 taatatacta ttgcaaaaaa taattcaaga gaaatagcct ttgtcaggca taaaaattgc
     3361 attatctctt ttcgaacgca tgaagcgttt taacactaac ccacaagatg tgaggaaaca
     3421 aaaatgacag atctttcagc caataccaac atcggcgcca tggccgcccg tcgttacatg
     3481 gaacgcaaca accgcatggc cagtcttgcg acgcaagaac ttgcttcggg tatcgtgacg
     3541 tatgatccgt cccgcaaacc ttctgagtat gcgatcggtg cgcgccttga tgcgactatt
     3601 agcaaccttg ctcaggcgac aaaaaacgca aatcagttga ttgccttgat tcagatgggt
     3661 gcgggtacgc ttgacacaat gcaaaacgtt attacgcgta tgcaggccct tacaagccaa
     3721 gcaaacaacg gcgcacttga cgacccttcg cgtgcgatgc ttgaccaaga gtatcaacag
     3781 cttttagctc aaatcacaaa cctttccaca ttcacccgtt ggggaagtac acagctcttt
     3841 aatggttctg cggcaagctt ggctgcaaat ggtgctgtaa cacaggcttt tactgtaaac
     3901 gcagtcacaa atgcctttac cggtacattt gcaacaggtg atactcaagg tgccattgac
     3961 ggtacagttg caagcgctac ggttacggct aacggtgcta actttaacat tgctgtgaca
     4021 ttccaagatg gacgcgtttt tagcaatact gttgccgccc ctgtcgcaaa cgatgtgctg
     4081 cttcttaaaa atcaagcaga cggttttagt accattggat taacctatgc tgcgactgta
     4141 acagcctttg acggtacagc cgccaccttc caaaatcttt tgaaacaaca acttggtatt
     4201 ggaacaggaa atacttcttt tggtacaagc tcttctgtgg ctatgtgggc gggtgcaacc
     4261 gtcaccccag gtgcatctgc ctctgcaggt attagttcat taaactatac agtgagtaat
     4321 ggtattggaa cctttacggt cacaaacggt cttaagtctg cgtcgacctc agtcacagca
     4381 agtgcctcta tgacagaaac aatcactgtg ggcgacttta cacttgctct taacgcattt
     4441 aacggcgctg caaaccttgc tcaggttggt gtaacagtca cagctgcctc aacactttct
     4501 atatcgggtc agatcgaaca gcattcctcg gatattgtga cgattcactt taaagccgtc
     4561 aacgcagctt ctcttcaact tgcgggaaca aacgttgcaa caattgcaaa tgctggaaca
     4621 gccaacatag ctctcaacgc tgctgctgcc actgttagta atcttgcggc acagcttggt
     4681 ggtacgcgaa gtcagcttga cttccaagtc gataacctta agatttcaca acaaaaccaa
     4741 tcagccgcaa aaagtaccta tacagacaca aacattgctg acgccacgga acggttgacg
     4801 aaattcaaag ctctttccag tatgtcaagt tctgtgttct cacaggccgt gcaagacacg
     4861 caacgtctag cacagcttct tgatcgtatt agttaataaa cttctaagag gtaggcaact
     4921 tttgcctacc tctttttttc aatgaggcaa aatattccat ggttgatatt gcaacaaata
     4981 atttgggtac ctcacagagc ctcatccaac aacgtacaga ccctttgtca ggtgccaaac
     5041 gttttttagt ctcaactaaa ttacaggatt caaaatttga cccaaatgag tatgcaaagg
     5101 cccttgggga tattaagcgc gaagaagcgc gcatcatcca agataaaatt gatgataatc
     5161 ttaaaaaaat tagcaaactc agggaactca aagaaaacac agtctctgtc agccgttttg
     5221 caaaaagtct cagtaattat ctggggccca atacagacac tccaaacctt tgggcgcaaa
     5281 gataccccac aacatttaat actaccggcc tggacatgag cgctcaggtg cgtgtcaccg
     5341 ccacaaacaa tgcaaatttt caaagtttta aaataaatgt catacaaatg gcctcaagtg
     5401 accaagttct cagcacaaaa acttttgcaa acacaacaac gggccttagc cttttaggca
     5461 atcttgtgat taatggcaca acaattccta taactgacgg gagcaatggc tccattaata
     5521 tgtctctttt ggatatttca aatgcgatta accttgcttt tcctaataac accatgcaaa
     5581 tgaatctttt taccgtggat ggtgtgaact acacgtggca gattacaacc ccctctcttt
     5641 ctcttcctat tgacctcaca ggaacaagtc aaacaattct tcaaggtctt ggtgttccac
     5701 tcaatacatc caataatact ttgcctgctg tgacaccatt gaacaacctc ttgcttaatt
     5761 ttacctataa tgggctcaat atcacacgct caggaacaaa cattgtctct gatcttattc
     5821 cgaatgtgac aattgaagtg aatgctgcaa atacagggcc actcacatgt actattgatt
     5881 acgatagact cgtcctccaa acagggtttg gtgagtttgt caaagcatat aatactctac
     5941 aaaaatttgc ccgggatcaa acggcctacg aggctgataa aattacacct aaggaagggg
     6001 cttgtcttca tcgaagtagt gttttaaaat cagaaatttt tatgctcaca ggtcttttgt
     6061 cagcgtgggt cccaggcgtt agtaatacgc aacccaataa cttaagcgct attggcctct
     6121 tggttggggg aaaatctaca gggtatatga accttgacag gacaaacgat atttatcttg
     6181 atgagcctac gcttgatcag ctcattaata gcgatagtat tactcaagtt ttgaccgttg
     6241 ttggcaatca agtttcttca agtaatcctt attttcgtgt tttctcagca cctgactttg
     6301 tgcaagatga tacgctttct ggagttccta tcaccttttc tgtccaaaat gcaaatggca
     6361 cccttgttgg gacattcagt gccacagtca atggtacgac catcacagcc ccagctacac
     6421 ttcttgaggg aggccttttc caaggtcctc ctattgtcga gggcacgcca atttcaccat
     6481 ttaatggcat aaacatcggt cttaaaacca cacagtccta ctctacaaat tcttttttta
     6541 gtgtctcctc aacacccaat tttatccaaa atagcaccct ttcaggtgtc cctgtcacgc
     6601 tttctgtgca aaacgtcaac ggcagttttg ttggaacatt gagtgcaacg attaatggct
     6661 cccttgtgac agaaacagcc acaacgttgg ctaatggcgt ttttcaagga gcttctggcg
     6721 gaaattttaa tgggcttgcc atcatgctga atacgacaga gatgaatagt ctcaccgtta
     6781 atggtgcccc tatcagtaca acaattactg ccacagcgct tgatggcatt aatattaatg
     6841 gcacaactgt aagcacaacc gtcacagcga caagaggtct ccttgataga ctccaagaaa
     6901 tgacagatag ctatatcgat taccaaacag gaaacatcga catagaaatc caaaatcttg
     6961 aggataaaaa ttatcgtgat cacgaaaaag tagaaagact taaaattgag gcagctcgcg
     7021 aagaagaacg gatcattctt ctttttaatc gcgtccgtga actcgagggg tattacaaag
     7081 aaatgacgga tatgatggat atctatacag agcttctcac agcacgcaat taaaggacag
     7141 taactttatg aataaagttt taaagcacta tcagcaccat aaaaaccgct ctaaaacagg
     7201 agctcaaatg gttgctcttg tttttaatca atgcagtgtg tgggttgaga acattgctca
     7261 aagcctttta gaaaaagatt attatgggcg ctatcaagct tctgaaaagg tcattatggc
     7321 actgactaac ctcatacctt tgtttgacac ctccacaaag gaacaggaaa tatttgccaa
     7381 ggatatccat ggtttttgct ttcttatcct cgcgaatatt acagatatta atcaaaaaga
     7441 ggattacaca ctctgtcttc aaacccgcca acttttaaaa gaaatggccg atatttggca
     7501 aaaaatttaa aatacattag aataaaggct cctatctttt ttaaaaattt tttatgcacc
     7561 tactcacaaa ccctaacgat attgaaacgc tctatgaatc ttttagagaa agcaagcaat
     7621 cgtttatcgc aatggataca gaatttattc ggcaaaaaac tttttaccct gagctttgcc
     7681 tcattcaaat tgcctttgac aatacctgcg ccattgttga caccctttcc ccgacgtggc
     7741 acccaaaaac cctcaaaccc tttatttttg aggattttct tacccttgtt tttcactcct
     7801 gtcgtcaaga cctcgaggtt ctctttcacc tttataaaga gcttccctct tatatttttg
     7861 atacgcaaat cgcagccgtt tttctagggc tctcgacaca ccttggaagc agtttgtcgt
     7921 atcacgacct tgtgaaacat tatttaggtc tttccattga taaatcccaa caacacactg
     7981 attggatgag acgccccttg gcctttgagc aggtcaaata cgctgctgaa gatgttgttt
     8041 atctcgcgga tatttatcct atgatcctga gtgacctcga tcatcacgga cgtttgaagt
     8101 gggcgcttga tgagatgcga agcctttcct cccctgactc gataacctcc tccacgccca
     8161 gtcgcctttc gcggatgggc atttataaaa aaaactatgc gtgggcaaag gctctttttg
     8221 cctttcagca taagcatgcc agtcgtctga atatgaatcg ctctcgcttt ttaagtgaca
     8281 aagctatcac ggcaatgagc ctctgccaaa gcaaagaaaa attaattgat tttgcaaaac
     8341 gtgcgacatc tcttgaagcc tataacctcc tcgaggagct gtgcgatgtt ttcgatcata
     8401 cgaaagatca aaaagatgat gacccacttg cgatgccttt gacaacatct cagcaaaaaa
     8461 ccctttcaga attgcgcaaa cacagtgaaa aatgtgcaaa agagcttaat ctcccgcctt
     8521 cattcttagc gaatgcagaa gacctccgtc tttatgtgct ccaaaaaaaa ggtcggcttt
     8581 taaccacatg gcgcaaggag gctctgggat tattgtgaca aacgttatga atcgatccac
     8641 aatgccgcgc actatgctgc gctatgccat tgaaaaattt ccaaaagaac agcgcgagga
     8701 ttttatggct agagactcga ttttatagaa ttctcttttt tgggcacgta aatataataa
     8761 tccctgattt tccctgtttt gggggcagta acgccttgac gtgataaatc cctcttaaat
     8821 cctaattttt ccaagacttt ttgggatcct ttgttttcag gatgaaccgt tgcaattatt
     8881 ttaatatatg gattgtcaat agcgtcgatc acaagacgtg ccgcttctgt ggcgatacct
     8941 aacccactca aatgggggtc aatcacataa ccaatttcaa acgcattttc ttttctgtga
     9001 ggataccaaa cggtcaatag acccgataca ccggcatccg ttaaaatagt aaaggtataa
     9061 tgctctctgt cagggttttg attattaagc acattttttt cgacaatttt tttaatatca
     9121 tctttactat atattttttc ttgaccgtaa tacttcatgg atgtttcatc accaaaaata
     9181 ggcgcgagtc ccttttcatc ccctttttga atggggcgca gaagaacgtt tgagccctct
     9241 ttgtaaagaa tatcttttag cttgctttca ttttcaatag cgtgagcgag taaaaaagat
     9301 gtgctgccca taaacacact cataatcatc gtaataatta aataaaacat tttaatgctt
     9361 gaattgtatt tttattgatt tttatatcag cattggtgga agatcaatag ctattcgaca
     9421 aggactccat cttttaaaat gacctgccgt gtcatttttt gagcaagatc catgttgtgc
     9481 gttgcaataa gcgctgcgac atttttttcc ttcgccagcg ctttaaactc atcaaaaaca
     9541 atatcggagg tgtggggatc aagattgcct gtgggttcat ctgcgagcac gatgggcggg
     9601 cggaggatga atgcgcgggc aagtgcaacg cgctgctgtt ctccgcccga gagctggaca
     9661 ggataatgat gcatgcgatc gcgaagccca acgctttcga
//
LOCUS       DAIAHG010000026         9431 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_26_length_9431_cov_3.737011, whole genome shotgun sequence.
ACCESSION   DAIAHG010000026 DAIAHG010000000
VERSION     DAIAHG010000026.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26_length_9431_cov_3.737011"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2579 a         2088 c         2025 g         2739 t
ORIGIN      
        1 gcgttgaaaa aagcaaatgt tttggcaaag cagttagctg agggcatgaa tgtcaaaata
       61 aaagacttgc gtagcacaga gcagaaagag ccagtgagca ttatctccca agatcaatcg
      121 ggaggtgagg ggagtcgctg ggggatggag cgcctcaaag gccctattaa gattggcgtt
      181 tttcaaatga atgttaagtt caatattgag tgaaaaatga taaagtaaaa atagtttttt
      241 tgatgttttt gatggcagca agagataaaa gccttgctgc tttttcctcg actggcttat
      301 aaataaaagc acggttaact tattctcaac tatcaaaaac tatgacgcac ctttatcgca
      361 cccatacctg tggtgagctc acacttcagc acgttgatac tactattcgt ctgtctggat
      421 ggattcaccg caaacgcgat catggccagc ttttgtttat tgatctgcgc gatcattatg
      481 gcattacaca atgcgttgtc gagagtgact tagaggcctt tgatattctt gataaactgt
      541 ccaacgagag tgttgtctgt attgacggcg ttgttgtggc gagaagccca gagacgcgta
      601 atgcaaaaat gacaacgggt gatattgaga ttgcgattaa aaatgtaaat gtactatcac
      661 ggtctgaaac gctgccgctg cctgttaatt tggatgtgga tttccctgag gaaacacgcc
      721 tgacttatcg ctttttagat ctgcgccgcg agaaattaca taaaaatatc gtcctgcgcg
      781 ctcacgttat tgcaagtatt cgccgtcgca tgatcgagca aggatttact gagtttcaaa
      841 cccctattct tacggcgtct tcgcctgagg gcgcgcggga ttatttagtg cccagccgtg
      901 ttcatccagg acatttttat gcgctgcccc aagcgcctca aatgtttaaa cagcttttga
      961 tggtggcagg gttcgatcgt tattttcaaa ttgcaccgtg ctttcgtgac gaggatggcc
     1021 gcgccgaccg ctctcccggc gagttttatc aactcgatat ggaaatgtct tttgtaaccc
     1081 aagaggatgt ttttaatgcg attggccccg tgcttacggg tgtgttcaaa gaatttgcaa
     1141 aagatgcgac tgtcgatgat tatccttatg cgcgtattcc ttttcatgag gcgatgctca
     1201 aatacggttc tgataagccc gatttgcgca accctctttt gattgcggat gttacggata
     1261 tttttgccgc cagcgagttt gccgcgttta aagacgcgat tcatcagggg gcagttgtac
     1321 gcgctattcc aggaccagga tgtgcttcga aatcacgaaa attttttgat gacttaaata
     1381 attgggccaa ggatcaaggg cttcccggtc ttgggtatat tacttttgcc gaagcagagg
     1441 ccaagggacc cattgttaaa tttctctcca atgaacagct ccaagacctt aaaacaacgg
     1501 cgaatgtggg tgaaggtgat tctgttttct ttatttgcca taaaaatgca tggaagtatg
     1561 cgggcttttt gcgcacaaaa cttggtgcag atcttgatct tacggaaaaa aatgcttttc
     1621 gcttttgctg ggtcgttgat tatccaatgt atgaaatgga tgaggataca ggcaaaattg
     1681 acttttcgca caatcccttt tccatgcccc aaggcggcct tgaagcgttg aatacgcaag
     1741 atccactaac gatcaaagcg tatcaatatg atatcgtgtg taacggcgta gagctttgtt
     1801 ccggtgcaat tcgcaaccac ctccccgaag tcatgtacaa agcgtttaac ctcgtaggct
     1861 ataccccaga ggatgttgaa gcgcgctttg gtgggctttt gaatgcgttt aagtatggtg
     1921 ctccccccca cggcggctgt gcccctggga ttgatcgcat cgtgatgttg cttgcgcatg
     1981 aaccaaatat ccgcgaagtg attgcatttc cgttaaatca acaggcgcaa gaccttctga
     2041 tgcaagcccc ttcgcccaca acaccagagc gcctcaaaga attgcatttg gagattaagc
     2101 tgccacgacg cgcgcaagat aaaaaataac tttttgaaaa gagagaaaaa tatgttttta
     2161 aaactctatt tcctgatcgc cgctttcttt cttacagaag tgcacgcctc taataaatct
     2221 gatgacgaga ataattttgt cttagaaaat gagaaaatca aaggtcgcgg gattgagcag
     2281 attcaaaaaa aaattgatca gcttaatctt caaaaaaaaa gaaattgata aaaaaataga
     2341 gcaagcgtgt aagaataatg aattatataa tggtattatt tatgaggatt ggaatgcagg
     2401 agctttgaat gataaggagc atacctcttt tcgcgaactc cttgatacaa agggatccct
     2461 ctacagtcaa atttctaaac ttacaaaaga aattaaagag atacaaaatc aaaattttat
     2521 atccaataaa aaaagaaaac ttaattaatt tccgtccaaa cacgtgtttt ttttaaagcg
     2581 gttgtcgcac tatttttttg acaaacccct gaaaaagcgc tatgttttcc tcgtatttgg
     2641 cggagtagct cagttggtta gagcagcgga atcataatcc gcgtgtcggg ggttcgagtc
     2701 cctcctccgc taccacatat tttcaaacaa tttttttgat tattcacgct ttcttaactt
     2761 ttttggtcta ttctaaagca atgagcaatg cttccttgcg atgggatgct tgtcgtttta
     2821 gcgttaacat atcgaaaaaa ttaagggaga ttgactgtgg ctgaagcaca cccccccaaa
     2881 acagtaacgg ttacctatga aggtaagacc tttgaaatgc ccgtactaag cggcacatgc
     2941 ggcccagatg tcgtggatat tagtcaattt cccaaacagt gcggtctttt tacctatgac
     3001 ccgggatttg ttgccacggc cagttgtgag tcaggcatta cttttattga tggcgacgaa
     3061 ggtattttgc tttaccgcgg ctattcgatt gatgagcttg ctcagcacag cgactttatg
     3121 gaagttgcgt atttactcct caacggagag cttccctccg tccaacaaaa acaggaattt
     3181 gttcataaca ttaccattca cacccttctt cacgatcaaa tgcgcacttt ttttcaagga
     3241 ttcagacgcg acgctcaccc tatggccatt atggtgggcg ttgtgggcgc actctctgct
     3301 ttttatcatg acagccttga catccatgat cctaaacagc gtgaacttgc ctcctaccgt
     3361 atgattgcaa agatgcccac gatggcggct atggcctata aatattctat tggccaacct
     3421 tttgtttatc cacgcaataa ccttgattac tcaacaaact ttttaaatat gatgttttca
     3481 acaccgtgtg aggattatgt tgttaatccc atttttgcaa aggccatgga tcttattttt
     3541 attcttcatg cggatcatga gcaaaatgcg tcaacctcta ctgtgcgcct tgctggatca
     3601 agccaggcca atccttttgc ctgtattgca gcgggagtag cggctctttg ggggcctgct
     3661 catgggggtg cgaatgaggc ggttttgaat atgctgagag acattggcca taaacgcaat
     3721 atccccgatg ccatcaagcg tgctaaaaat aaaaatgacc cttaccgcct tatgggattt
     3781 ggccacagag tctataaaaa ctatgatccg cgcgcgcgta ttatggccca agcctgccat
     3841 gatgttctga atgtccttgg aaaacaaggc gatcatctct tagaaatagc gatggagctc
     3901 gaggaaattg ctctgcaaga cccttatttt attgagcgca aactttatcc taatgtggat
     3961 ttttattcgg gtattatctt ccaagcgctt ggtattccta catctatgtt cacggttatt
     4021 tttgctcttg cgcgcacagc aggatgggtg gcgcattgga aagaaatgat tgcggatcct
     4081 atgcaaaaaa ttggtcgccc aagacagctt tacaaagggc ctgaaaagcg cgattatgtt
     4141 cctattcatc accgcgctca aaaacacaat aaacacgttg catccttttg atacgattga
     4201 catcgccgcg cttgctttta ttgaggagtg cggctatgtg cctacgctag attttttacg
     4261 aaaaaataaa gcccttggcc tccccaccga ttttttgaac aaagatcatg accttttaaa
     4321 attaattttt tatcacaaag aaaaatctct aaaagctcta aaatatacga taacaaacga
     4381 tttaaaagag cttttttttg atcacattat gcactattta gacctttggc aggagcataa
     4441 aaaagcgtac cggatcattc tctctcacag cttgaaatcc atggaaacgc tgccacttat
     4501 ctatcatcat cacacaaaaa ttctaaggta tcccttggaa aaaaagccag gttttccctc
     4561 ttgcaaacag gcgatttttc tgcatggcct ccttggcatt ttcaccctaa cacttttcac
     4621 atggtttgag gacacaacac tcgatcaagg gccgacaatg gcaaagttaa gtgagctttt
     4681 aaaaaaaagc gaaaatattt acaccttttc attttagaaa aatgctatgt ctattttaca
     4741 ctctccctat actcaagaga aatcaatgcg ccttattttt atgggaacgc ctacttttgc
     4801 tgtaccgccg ctgcaggctc ttctcgataa tggctatact atcgttgccg tttacactca
     4861 gcctccgcgc cctaaaaatc gtggacacca ccttgcacaa agccctgttc atgtgctcgc
     4921 cgagtctttt aatattcctg tttacacccc aacgacgctg cgcaacagcg aagaacaaat
     4981 tcttttcaaa caccaccacg cagacctcgc tattgtcgta gcctacggcc ttattttacc
     5041 cgcgcccatt ttacaggcgc cgcgtcttgg atgcatcaac attcatggat cgctcctccc
     5101 aagatggcgc ggtgcggcgc ctatccaccg ggcgatgctt gctggtgatg cacttacggg
     5161 tattacattg atgcacatgg atgaagggct tgatacaggc gatatgctca gcactgcgac
     5221 tcttcccctg gatattcatg atcattttga aaaagtgcat gatgccatga gtgatttagg
     5281 cgctaacctc ctcatccaaa cgcttcctgc gtttataaag ggtcaaataa cgcctcaaaa
     5341 gcaaccttct cacggcgtga cttatgccca taaaataact aaagatgaag gagtgattga
     5401 ttgggcgctt cctgcgacag atatcctgcg tcggattaag acgcttaacc cctggcctgg
     5461 aacagccact ctttacaaag gccaacccct taaaatccgt gaggcttctc tgcttgaaat
     5521 gcaagaaaaa catgattaca tgaccggaac acttctggat aatgcatgtg tgctttgcaa
     5581 agacggtatt ttatcccttg atgttttaca aaaagaaagc gggaaacctc tcttaaaaga
     5641 tgactttctt cgaggcttcc ctcttgaggt aggcacacta ttaggctgtt agtgcatgca
     5701 cttatcaatg tgctcaaagc ttacatgggt gtgcccgcta ttcccccctc cctggtgatg
     5761 cacgtttcct ccacaggcag aagcttcatg atgatggagg tctatggtca ttttgctccc
     5821 atctgcgcaa attacatggg cgtgatggtc atctccattg gtgaggtgca gccagtctcc
     5881 tcccccatga ccatttgact gatggtgatt ttcaatcatc atagggggat ggtgctcttt
     5941 tccgccaacg aggacgtgat cattacaatc actttgatga tgggttgtat catggggaga
     6001 gacactactt ccccctgagg catggtgaga tccactatgt tcatggccat tttgaggatg
     6061 ggttgtatca tggggagagg cgctacttcc ccctgaggca tggtgagacc cactatgttc
     6121 atggctgctt tgatgatggg ttgtatcatg gggagaggcg cttgaggatg agcttccacc
     6181 ggaggtgtga gacccactgt cggtactgct ggaggaagca ctgctacttg aggcggcact
     6241 gctggcagca ctacttcccc ctgaggcatg gtcggatccg ctatcactgc tactgctact
     6301 ggctgaggcg gcactgctgc tacttcctga ggacgcgcta gttccccctg cgtcggcgct
     6361 gggtgtattt tgagaactag gagtgttcat atggagacct gtctgatgta taccacctaa
     6421 aggtgttgtt tgaatgtgag gtgctagacc aggcgaaaga ccctgacccg aaattggtgt
     6481 ggtattgtcg aagtgcactt gactatttgg agcatcagta tgtgcactta gctcactaat
     6541 cgatgtatgt tttgttgtgc ctgaatggat ggattcaata atttcttgag cgacattttg
     6601 attggattga aaagaggcat cgtgctgact tccatcatta atagtttgta cactgttatt
     6661 gtgaaaagca gctgctccat caaaaacaat tccgctattc agcgatacat ctttcagggt
     6721 gctctggtgg tttgcttgag aatcatcacc ctttgcacct ttatcctgac caccccttaa
     6781 caatttttca tcatcagact gcatgctcac ataagaggga cctgtgtgag aaggtatcgt
     6841 acccttgttt ttatcataat cactcatagt aacctccctt taataatcta ttcatgaaaa
     6901 attaaagata aatagaattc tctaacatca aaaatataat aaataagtta atataaagta
     6961 actattgaat agcattttaa atagattgct gatatctcgc gaaattggta taaagaatct
     7021 atgatcttag agtcgcttaa acagggtgtg ggtgtcattc aaagcaccct tgaaacactt
     7081 tcgaacggcc ccggcgttta tcgcatgatg ggcgaggatg agagtgttct ttatgtaggc
     7141 aaggcaaaat ctcttaaaaa acgtgttgtc agctatacgc aagttgacaa actccctctt
     7201 cgtctcaaac gcatggtttc agaaacccgt cgtatggaaa ttgttgtcac ccatacagaa
     7261 acagaagcac ttttgcttga gagcaacctc attaaaaaac ttcagcccaa atataatatt
     7321 ctgctcaaag acgataaggc ctttccttac ctacatttaa cgcatcaccc ctatcctaaa
     7381 atggtgaagt atcgtggaaa aagagatcaa gaggggcggt tttttggtcc ttttgcctct
     7441 gctgctgctg tggatgaaac acttgtacag attcaaaaag tatttttatt aagaaactgt
     7501 agtgatgaag tgtttaaaca acgcaagcgt ccctgtctgc agtactacat caaaagatgt
     7561 acggcccctt gtgtgaattt tattgactgt tcgctctatc ttgaatcgtg tcaacaggct
     7621 gttgctttct tagagggaaa aaatgcggat gttcaacact atttaacctc caaaatgcaa
     7681 caagccagcg aagacatgcg ctatgaagaa gccgcagcct atcgtgatcg aattcaactc
     7741 cttaatcata ttcagaccca tcagcgcatt catcatcatg atatcaaaga agcggatatt
     7801 atcgctcttg tggcacatca gggtaaagtc tgtgttcaga ttttcttttt tcgttttggc
     7861 agcaattatg gcacggaatc ctttttcctc gaccatataa gcgactccag tgcagaaaat
     7921 ttaggtgctt tcctgaagca attttatgag aaacgtgaac ctgctgagct cattctttta
     7981 agtcacgcac ctgaggattt taaagcgatt caaaaggcca ttagtgattg ctacaaaaca
     8041 aaaatttcgt ggcaaattcc aaagttaggc ccaaaaaaag aattaatcga ccatgctgtc
     8101 ttgaatgcac agcaggctat tgagcgcgtc cttattcaca aagcaagttt taaaaaaact
     8161 tttgaggaga ttgccacact ctttggcctt gatacaatcc ccaatcgcat tgaaatttat
     8221 gataatagcc acttatttgg gaaaaatgcg tatggtgtta tggttgtcgc ccatcaagat
     8281 ggttttgata aaaaagcgta ccggaaattt atcattaaaa atcctggcaa taaactcaca
     8341 gggggagatg attatgccat gatgcgcgaa gtgatggagc gccgctttca tcacattgag
     8401 gaagaaggat ggcaaagacc tgatcttttg ctgatcgatg gaggtcttgg tcaggtcaat
     8461 agtgtcaaag aagtcctcac tgaaaaaggc cttgctgata ttcctgttgt tggaatcgcc
     8521 aaagggcccg accgtaatgc gggacgagag gaatttatta tccccggacg cgattctttt
     8581 caacttcctc atcattcccc cacactacat ttgctgcaac gccttaggga cgaagcccac
     8641 cgttttggga ttagcgcaca cagatcagca cgggaaaaaa atctgcgcac atcgattctt
     8701 gatagcattc caaatattgg acctaaacgt aaaaaagcac ttcttttgca ctttggatca
     8761 gcccaacatg tggctcaagc agcactagag gaccttctta aagttccagg aattgataaa
     8821 tgtgttgcaa aaaaaatcta tcagtatttt catgattctt agttttaaga tgaccttaat
     8881 ctctttgtcc ttctaaccaa tcaccttcga tgttatgagt tttatgtgaa aacacttccc
     8941 aatcttctca cacttcttcg catactcctt atccctgtga tgatgggagc tttttataca
     9001 caaagtacaa aaggcgcatg ggtggcggcc gtttgtttta ttcttgcaag cattacagat
     9061 tatttggatg ggcttgcagc gcgccttctc tctcaaacct caaggcttgg tcaattttta
     9121 gatccaacag cagacaaact tttgatcgtc tccacacttt tcctccttgc agggtttcat
     9181 aaaataagcc ccttaagttt tattcccgcg actattattc tttgccgtga aattatggtt
     9241 tccggtctgc gcgagattct tttagagctc aaagttccct tacccgtgac acgccttgcc
     9301 aagtggaaaa cggccattca aatgcttgct ctcagcattc ttttgataag tgatgctctt
     9361 aatcccccaa atgatattca tgctcttggt gaaactctct tgtggattgc aggtattctt
     9421 actcttgtga c
//
LOCUS       DAIAHG010000027         9348 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_27_length_9348_cov_3.063531, whole genome shotgun sequence.
ACCESSION   DAIAHG010000027 DAIAHG010000000
VERSION     DAIAHG010000027.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9348)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27_length_9348_cov_3.063531"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    9033..9042
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2746 a         1627 c         1985 g         2980 t
ORIGIN      
        1 tatgatctta gaatctgctt catcagcagt agactcactc atattactgg aatccgtatt
       61 attggaaacc attgtatctt taggaaaagt ttcaaaggga gctggtgcat ctttgggtgt
      121 tgtactaaaa atatagaaaa tgaggcacag tgataaaaaa cttaaagcga gggagagcgt
      181 aataataaaa cgatttggtt ttgggattga gctctcgtga ataaaagggg actgtgagag
      241 gggattttcc ttggcaaggc tgaggcgacg taagatttct tccccatcga gactcagata
      301 gcgtgcataa agcttaataa atcctgcggc ataaagagca cttggaagac ggtgaaattc
      361 acctttttca atggcttcaa ggtaaagggt gcgtacatga attttatcgg caacggcttt
      421 gacggataaa tttagctctt ttcgtgcctc gataaaaaga gaaccaatgt gttgattttt
      481 agcgtcttgc tcggcgatag acgtctctat aggcgcatgc acatcacaat catccggtgt
      541 attgtgtgct aaattttgca taacagcctt gctctacaga tataccacat gatgatagca
      601 ccaagaatcg tgatggaaaa gagatagttt taatctctat aaaaacgcct gaaaagctct
      661 attttctcga catttttcaa ggatccttgt attttttgga gcaatattaa ttttaaaata
      721 attgagatta tggagcctga gaaacaacgg caacgaaagc tttgggggcg ctcgctttca
      781 aggcctcttt cgccaacaca aaaagatttg tttgataaaa gtatggatac attttcatac
      841 acgcatcatc atatcgattt ttttcaaaaa tataatgcat ttgttttaga aattggtttt
      901 ggagatggag agcatttatt agggatggcc cttgagaatc caaccatggg ttttattggg
      961 tgcgagcgtt ttatcaacgg tgtggcgaat cttttcaaag gcatagagga gcatcagctg
     1021 aagaacatac gtgtttttaa tgatgatgtg cattttttaa tcgaagattt gcccaaaaat
     1081 aaattggaac gtatttttgt tctttttcca gatccttggc cgaaaaaaaa acaccacaag
     1141 cgtcgtcttt taactaaaga atttatgggg ttgtgtcata cacttcttgc ccctggagga
     1201 aaactgtgta tggcctcgga tattgaggat taccggaatt ttattcaaga agaggcggca
     1261 caggtacaag atcttttcgt gcttagagaa acgatatcgg cgcctatgac aaaatattgc
     1321 cgcaaggcgg aaaaggccgg gcgtcattcc ctcatgtcta tttacgagaa aaaagaataa
     1381 ttaatgttaa aaggcacagg tccttgcgcg tggcgatttt ttttaaataa cttctttcag
     1441 ccactccttt aaatttgaag ggaaatccag ctcagaggcg atctttgtca tgatttcatt
     1501 ggctatattg gtttgagagt agttgaagtt aatctcatac ttattttcct tcatcggcac
     1561 ctcaatggtt tcatcatcct tgtatgtgta ggccttataa aatgattcgt taaattctaa
     1621 ggacaattct ttacccgtaa tttccttaag gaaaatgttt aaaaccgata attttgattc
     1681 tatgtccttt gttagcttac aggtatcatc ttttggatat atataattga aattgaactc
     1741 aaaccaacgc ggatgacttt gaagtttgag tgttaagatc tctggatgtc cctcggtctc
     1801 atcaaagact gtgaaatcat gttctttttt cttctcatca gagtaaataa ttttgatagt
     1861 tgaataagat tgttgttttt tggcggagaa aggggagggc tcatatcgaa aacagtcagt
     1921 cctattggat ttaaaaaaat aagaggtttt ggggagtgtt aatttttctt ctttttttca
     1981 atttccgttg caacggtgag ctgtgtgctg gatagaaaaa gagtcaggaa aataagcaga
     2041 taggaagact tatagcgcat gatagaatcc cttagggtga tgattaacct taattatggt
     2101 aactctgttt tttccctacc tcaaggggat gacatccgcc gaggagatgg tcagtggaat
     2161 agtgcgcacc gtttgtgtcg ttttttcggg cctatgagct ttgcgcttaa attcgatggg
     2221 aaaaacgtgg gcttctcctg aaacggcccc agaatttctt ttggggtgag tgccagtttc
     2281 ttttttccta tgatcatcga ccattttaag aggaaaaaaa gtcacggtaa tggggatggt
     2341 tttattttgc tgtgtgagaa gattcgtttg aatgctccca tttcttgggg ggacacgact
     2401 aaagtcaagg ggagaggatc taagcttttc tgcaccgtga ataagcccgc ccattgtctt
     2461 aatgtgagcc caggctttaa gaactttttg gagataactg tgattaagct ccgcattgcc
     2521 cgagtgataa tgtcttaccg ctcggtgcca cgaaccatgt ttgaggtaga gattatagag
     2581 gaaattggca gcatagctaa tgttgtgcac aggatcgaac gcatcctcaa gacttttaaa
     2641 ggcgcgcggg tgatgtttaa gattaatttg catcaaccca atatcaatac tatcgtagcc
     2701 tcttttttga aaattgcgca cagcttgaac cgcttcgact tttgttttaa aagagtaacc
     2761 tttaccgtgc acgttaatcg tccagggtct gacctcgtgg tgattttttt gtctgtagcc
     2821 agattccaca caggcaatgg ccgcaagaag tttttcagga attctttttt gagtctcgac
     2881 agagtgcaaa atatgggtat agttcgcctc taatttttgc atgctgatca aaagaatcat
     2941 acataaaacc cagagcatca gtaacttcgc tttttttcat gacatgtact aagaaaggtg
     3001 caatttccgt gccaagggag gggctattga gattttttat gtaaaagcac ggaaaaatct
     3061 cttgaagaaa gacttataaa taagcatctt aaccttagca ttgatcaaaa ttgggaaaga
     3121 aaatggcaag tcagcgacaa cttcgcgtcg gggaagaaat acggcatatc ctggccaatc
     3181 tgattatgcg taatgattgg ccgaaaggag agctttcgaa tcctgtaaca gtgacgcgcg
     3241 tcgatatttc tcccgatttg caaaatgccg tagttctcgt gatgcccctt ggcgggttaa
     3301 aacgcgagga aagcatggct tttttaacag aaatggcgcc ctatatgcgt aaatatttag
     3361 ccgatcacat ggggttgcgc cgtgtgcctt atcttaaatt tcttctcgat gagaccttcg
     3421 ataaggcgga gaaaatggat cgactgttaa aaagtgttca ataaaaactt agttttttga
     3481 ttttttattc aacttggttt tcgttttttt cccataaact tcttgctttt gggttctttt
     3541 attaatttta aaaatatctt tttccatctt tttttggacg aaagatgtca gcagaatatt
     3601 ttgataagtg gctgttttca gcatatccct aaggtttttt ttggcaatat cttctttttt
     3661 gggaggagaa agtgtgatgt tgtctaccct tttttcaagc aaccttagcg cttgatgaat
     3721 atcgcttagc gtcttttgat agcttaggag aaggttgtta tttttattga ccatatcgta
     3781 atgactatta ttacaaacaa cgactgttaa aaacagtgtc gttaaaatgg gcataacaac
     3841 agagccacat ccaatcatac ttgaacacgt tagcttatca ctcaattctt tttttgtgtt
     3901 ttcacagttt ttttcgatgc tatataacct cttttcgatc ttctctatct cctcaagcgg
     3961 gcttttttca tgagcatcat tcttgactga aaaagaaaaa acgagcccac tcatgctgat
     4021 aaaagcaggg agaagatact tgcaaattga ggtgtacata agagcttttt catgaaaggg
     4081 atgttgtgct ctttttatta tgaatctatt atgtttaata aaatgttaac tatagataaa
     4141 attctttatt ttttcatcca atgataatgg gcaaagacca tagttgaaaa agccatcaat
     4201 aaagccaaaa caaaagctcc ttgagtgctt gtgtgatcaa gaaggctgat gctcagaaaa
     4261 acccccgtaa caaggccata aataattttt ccaagggata gatttttttc ttgttgcaag
     4321 ataatgagca agagtccaaa cccaatgcca gagataaagc gccaaaaaac aaagtcaata
     4381 gcgttgtaag ctaaaagctc acccataatg ccaagcccat aaaaaccaca gctataaaaa
     4441 ataggtttct tcatccgcgc tttaaaaaat gtttgaaaaa agtaagcaga aaaacatccg
     4501 gcaatgaata tgcaaaaagg cacaatagcc cctgcactgt gggattcact gttacagaga
     4561 atgaagaact ctagaaaact ttcggtgctt atgaaaagaa aaagggctag gtccaaaaaa
     4621 cctagtgata gagatgagcg cgtatgaaaa gagtttttac tattgatgtt tttttcaata
     4681 gcgtaaaatt ccttgggaag gggcgggaaa gaattatttt taagcgtatt ttggatagcg
     4741 actttggatt gaatgcgcaa aaagagtgta ttgattttcc gagtgaattt ataaaaaaaa
     4801 tggctgagct cttgaatttc atctgtgctt tttggtgtat attcatgata ggtgtaatga
     4861 ttttgagcac attcctcaaa ggattttttg agatcaacaa gaggcctttt gatggaattt
     4921 tcgcttatag cgtaaaaaat ctgagttgtg aggatgcatg taaacagaca aagaaaaaag
     4981 agctgtgata gttgccaaaa attttctttt gaccactcta tattcatatt aattttgaga
     5041 aaccctaagg tttttccttg ataatcaaag ggaatttcta tttcttgagg attttttata
     5101 gggctgggct tcttttgtga ggcatgatag agtttgtgat aatttgagtc ataaaaagca
     5161 atgtttgtga tttcttgatc accctctaag gctgcctgaa agtgatcatt agcgtagcgt
     5221 atctcgtgca tattgatatt aagatcgatc gctttttcaa gaggtgcaag aagagctata
     5281 cccaaagtag aatatctttt ctctgtctca acagaaagac cctgtgtaat caatatattc
     5341 gcgctataga gcatgatgcc cacaaaaact aaacaaaaaa aacatccata gagcaaaaga
     5401 ttgcgctgaa taccttttaa agatgagggg aaaaaaaaat gcatttttta agctcttttc
     5461 tgtatctgtt cccattcctc ttcaaaagat ttttcttttt caaggacttg atcaattttt
     5521 gagagtattt ctgcaagttt gtgtgtgtgg cgatacatgg tgtaaaaact ttcctcaaga
     5581 gcattttctt gaataaatat gctggagcta ttgtgaatga catcgttcgt gagaagtatt
     5641 ttcattttat ggcaagatcg tgtaatgggt gcaaaaagcc tttgaatcag aaaaaaagag
     5701 agcataaaaa agaaaaaaag aatcgttaga gtcatcaacg ccattttttt agcaaaatca
     5761 aaaagagtgt agttgagagg ggtttgatca aaccgcacaa tgactccacc gataggttgt
     5821 ttaaagggat taaaaagggt gactcccact gtgccgatac catcatcaaa aatacgccag
     5881 agtgtttctt gatgagggtg attaattttt tcgagccaac ttagaggaat attcgcattt
     5941 ttactttgac tgcgcgtgtg gtaaagaatc cgcccaagat tttttgaata tgtttcaaaa
     6001 acaccaatgg agagaatgtc tgagtgtttc ttttttatcg catcaatcac agattgtgtc
     6061 gtattgagct cactcaaaga gagacccagg ttgagcccat cagaaagagt tttgctaatg
     6121 tcttgggcaa taatttgaaa tctacttgtg gttaaatcag aaatttcctt tttataaatg
     6181 ctatacatga taacattaaa aacaacagcg atgaagagaa gagagacaat aagagctgct
     6241 gccgttttta aggaaagaga tactcttttg agaaaaaatt gcattgttgt ctatgcttag
     6301 aaagttttta aattattttt attaaatgct aagctattca gaagcgactg acaatatgca
     6361 agttttataa aaaattctct ccctcgaaaa gggagagaat ggggggaact aataattttt
     6421 ttgagttttt gcggacttgt tattggttgt ttcactgttg ctattgcttt cctcggtgga
     6481 agcttgagct tcggaaatgc tggtattgct tccaacgctt tggctttttg tgccagacat
     6541 agccttaggc tcatccattt tcttttcacc cattttggca tcttctacct taggttcatc
     6601 tgttttcttg ggttcttcca tttttggtga ttctttctct tcgccttttt tctcttcttt
     6661 gttgcacgcg acgaatgcta aagaaatacc gagtaaaaga gcaagtgata cgattttcat
     6721 gataaataat ctcctaaaaa agttaaactg ccgcgcattg cgactctctc atcattcagg
     6781 ctagcattaa aatcttaaca aaggattaaa aaatcggctt ttttatgtac aatgtttaga
     6841 ccattcatat tttttatacg tgcgtgttaa aaaatgacta tcgtcatacg cggggccagg
     6901 gaaagtgatc gagacgtgct tgaaaatctt ttccgaagag tgcgccagaa aacatttaga
     6961 aaaaggccgc caggcgtatt taaaatcggg gactatacag tgtcaacgga gggagaaatc
     7021 gtgtgggtgg cgcttaaaaa taacgctatt gttggttttg tatccatata tttaccgcat
     7081 tttattcatc atctttttgt tgatccatgc gctcaaaaac aaggcgttgg ggcgctatta
     7141 ttgaaaaaag cagaagaaaa tttgaagcgt cctatggagc ttaaaatagc ccttgataac
     7201 aaaggcgtgt gtcgttttta tcaaaaacaa ggctgggtgg agatttcttt gcatgatcag
     7261 gcgccagagc cctatcttct ctatagaaaa tattgaaaaa gaccaaagac ttgaaaaaat
     7321 cagggaaaag gagcattctt ggcgctgtac tcatagcaaa ttaatagtga tgcttctttt
     7381 tttaacgatt gttttgatcg attttttggt ggcggctgag atggatattc tcgttccgtc
     7441 ttttccggca ttgcaacagc aattttcact ttctccgttt atggttcagt tcgttttaag
     7501 tgccaatggt gtattttatg cgctgagctc actttttatg gggcctcttg gcgatcgcat
     7561 ggggcgtaag caagtgattt tgggggggct ttttgtcttt atcgcaggat gttttttatg
     7621 ctttacagcc acgtcctata cgatggttat tgtggggcgt attttacaag gcattggtat
     7681 gtctgggtcg gctgtccttg tttttgtcat tgtctcggat gagtatccca aagaaaaaca
     7741 agccactatt ttctccagcc tgaatggggt gatcaccctt gggatggctc tatctcccct
     7801 cattggaagt tacgttaatc ttcggtttgg atggaaagga aatatcggat ttctttttgt
     7861 tttggcgatc attagtctta ctttttgcac tctttttgtg cctaaaagac cgctcaatat
     7921 aaaaaagaat gtgcagtttt cgctgatgag ctatgcgcct ctctttaaat ccaaagtttt
     7981 tttaaggctt gctttgacgg tgtgtcttct ggatggctgc tattggatat ttgtaggcat
     8041 gtcatccatt ttatatgtcg aagatatggg cgtacctctc gatcaatttt ggtattacca
     8101 gggggctgtg gccggagctt ttgccctttc aagccttttg aatccgcttt tatttaagta
     8161 tattcccccc aaaaaatgct tggtttggag tctttacgct tctctttttt ttgggtttgc
     8221 catggtcatt atggggattt ttgtcaaaga tacccccctc tatattagca ttagtttttg
     8281 tcttttttct gcggctttgg tttttccgct taatattgct tacccaaaag ccattgcact
     8341 ttaccccgag tttaaaggaa gggccgctgc ctttattaat attgggattt taattttcac
     8401 agcaattggt cttcaggttg ttagtttttt ttatggtggc aatttcgtgt atctcgctct
     8461 ttttatgttt gtgtacattc tcatctcatt tattcttatg cggagtgagt tcaataatac
     8521 aaaaaataat cctaaattaa tgtttttttg acattttaaa tgccatttcc tatcgatgag
     8581 gctctttccc ccttagccta ttaaaggagc caaagaataa ttccccctaa aaaaatacga
     8641 tacagagcaa aaatgaggag actatgtcgt tgcaaaaagg taatcataaa atggatggct
     8701 gcgaggccaa agagaaaaga cagcgagaaa tagaggctaa ggtctagaag aggtgtttga
     8761 ataccttcct tggacgcatc cataaagatg aggagtgatg ccccaaggat tgcgggaatc
     8821 gcaaggagaa aggcgtaacg tgtggcgctc acacggtcaa aaccaaggag acgggcacct
     8881 gttaggcaca tgcccgaacg gctgctgccg ggaatgagtg aaatcacctg aaaaagccca
     8941 ataatcagag ctttcccaaa agtcattcct tgaagtgttt gatttttttg tgcgaagcga
     9001 tcggcggcat aaagaaaaat tcccccaaga atnnnnnnnn nngtacgacc tcaatgccga
     9061 tgcgtttaag tagaaaccct gcgataacta cggggaatgt tgcgatgata atagtgaagg
     9121 ccaagcgggc atgaaaagga tctcgagaac ggcctaactt taaataataa ccaaaaaaat
     9181 ggcgaatcat ttgaaaaata tctttataaa aatagcaaca cactgaacag agtgtaccaa
     9241 aatgcagaat gccatcaatt tctaagctat gatcttgcca accgaggagt gttggtaata
     9301 aaatcaaatg tccgctggat gaaacaggaa gaaattccgt aagtcctt
//
LOCUS       DAIAHG010000028         9249 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_28_length_9249_cov_3.122002, whole genome shotgun sequence.
ACCESSION   DAIAHG010000028 DAIAHG010000000
VERSION     DAIAHG010000028.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9249)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28_length_9249_cov_3.122002"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2813 a         1833 c         1963 g         2640 t
ORIGIN      
        1 aagagaaatt tttaaaaaaa atcttcgcaa aaacgcacct tatcccctct ttttcatcga
       61 tttttgtgaa aaattcaaaa aataaacata aaaatcccga aaaaccctag tttcatacga
      121 ctgttaagaa gggaaaattt ttctaaaaat cgcttacagc ctttgtgccc acatcaaaga
      181 cacgagagtc actcaataca gccccccctc cagcacaact atttttcact gaaatcccct
      241 cgaaatttta agagaaaact ttagacatcc tcacctaaac tcatctctag tcccgtatct
      301 cagcaaattt taaaagggtg agaggaggag aaatacccat ggattcataa gctgagcgtg
      361 aaataacaat ggaaaaacgg gataattttt catagggcat ggtccctgga tcttgtttat
      421 tcacaagaat atcaaaagct ttggcccctc cgaacatccc cacattaaaa aagtggctga
      481 acatgccaag catgggtttt gtatgccaat agagggattc agtcgcagca aatgttggaa
      541 ttttgagctc atgagctttg ttaataaaaa ttggagcatg ctttgagaga aaggtatcgg
      601 cgggaatata aataaaatcc gccttaagag atgctgcttt ttcaacaaca tcacagagat
      661 gatccgagtc gggcttgtga tcgatcatgg gcacaggcaa aagaattaca cgaatgccct
      721 tgccctcagc cagagttgcg agattttcca cttgaatttt tgaattgggt tcatcggggt
      781 tatacatagc tccaatggtt ttaacgtctt ttttatacaa aaggagagca ttcagctgtg
      841 cctcaatggg ggcaaggtga ctcacgcccg ttacgttccg ccccggcctg tccaacgcag
      901 caataatgcc tgttctgacg ggatctgaaa cgatcaaggc tataatgggg atatcccaaa
      961 tataattttc ttttgcctga tcgagacgcc cggcaatttc ttggagagcg ggggttcccc
     1021 aaattgttat cagatcaggc tgaatacgct taatatctgt gacaaaagca tgacactttg
     1081 cccggtctcc ctggcaatca agtgtttgaa tatcaacact cacctcttta ctcttaagat
     1141 aattgacaaa gccttcctca agcaaagtgc ttccgttcca aagaaccatg ataatcttaa
     1201 aagtcttttt ttcctccgca caaagagagg ccatcatcag acaaaaaata atgattattt
     1261 taaaagagct catgcgcatt taagtgccaa aagcccttga tacgctcatt tgacgattcc
     1321 aacgcacatc ccctgtgaca aaattacgga tatcattcac ttcatattta agcatcgcaa
     1381 agcgctcaac gcccatgcca aaggcaaagc cctgatgttt ttttggatca aggcccacat
     1441 tacgtaaaac ttgcgggtga atcatcccac accccagcac ctcaagccag cgaccatttc
     1501 caaaaatatc catctcaaag gagggctctg taaaaggaaa aaagcttgga cgaaagcgtg
     1561 ttttcaaatc atttttatta aaaaacgcac gacaaaaatc aatgagcgta ctcttcaaat
     1621 gtcccaaatg aataccttcc tctacaatga atccctcgat ttgatggaac atgggcaagt
     1681 gtgtcgcgtc ataatctgca cgatacacac gccctggtgc taaaacacgc aaaggaagct
     1741 cacctttttt aaaggtgcga atttgaactg tggaggtgtg tgtgcgcagc aacaaaggct
     1801 ccgcagcagc atcaacataa aaagtatcat gattttggcg cgctggatga tgcggaggaa
     1861 tattaagcgc attaaaatta tgctcttctg tttcaatatc tgggccttcc tcgaccttaa
     1921 agccaaagcg ctcaaaataa cgcacaatct cttctgtggt ttgggtgatc aggtgatgcg
     1981 tacctttttg aagcgccagt gtaggaagcg tcaaatccac tttctctgag gccaatttta
     2041 gggtaagcgc ctgttcttcg agtttttctt tttgaacact cagtgcctct tgacacaggg
     2101 cgcgtaagcg attgagttcc gcacctcttt gcttacgggt ttctggatcc attgccccaa
     2161 gagatttaag ggcctctgtg atcattcctt gcttgccgag atagcgcaca cgcagttcct
     2221 caagcatctg caagttttga gatttttcaa gcgcgaggtg aaaatttttt tctaaatcag
     2281 ccgtcataag tgtgcatatt tttttaaaag gatttctttt agtattagca aataaccctt
     2341 taaagtggct atgtttttca tagtttttca cgaaaaagag ccgctgtcaa aatattaacc
     2401 tttttttaat ttttgctctt ctaacatcaa ggtagttctt tctctcttaa aaaataagat
     2461 gttttttgat gtgctaaaaa ctcttttttt cagtgtgctc tgtcttgagt cctctctctt
     2521 gaatgcgtct gatcttcttg gcaatctggg ggcaagcaat ccttttcaat cgattcaaaa
     2581 cgcggattca agcgtagact caagtgtgga tttcggccca gagcttccga ttgacgatag
     2641 tgatgagtcc tttgagggca atcctattcg ttcagaaaat gaatttattg aggaatccgc
     2701 ccttgatagc acagaaaatg tagataccat tcaaaaaaaa ttcggcgggg gtggtttaaa
     2761 aagccctgcg caacaaactg aagatagcga tgaaagtgat caaactgata cggaggataa
     2821 caagaaaaaa agtagcggga aaaaagctaa atcggcgcct gaaaatatta aattaatcga
     2881 aattgacaag gcccttgacc ttaaaaaaaa gcacacgggc gatatttttt atctttctat
     2941 ggatcttgtc cacccccccg taaccatcta ttccaaagaa gcttttgagc actggtatac
     3001 acgcgagcag ccaggtcatc catcacttcc ctataacaaa aatcgttccg cccgtgatac
     3061 aaacatacct tttaaggtcg ttttagcccc caatcatgcg tatgttgtct ttgtggatac
     3121 gatcaatgaa tttcttaaag atcaaaaagc aggtgcaaag gccattcctg ttgagattat
     3181 ccaagacgat agtgacctgt atagtgaaac tattttttgg aacaagcttg agaaagacgg
     3241 tcaggcttgg ctgaaagata tcgatggcaa agaacaaaaa cccttgacgc attttaatca
     3301 gctaaaagat gaacctcttc ttggggcctt agacaaggcg attgttgcag tgtcttatgc
     3361 aagaacgccc ccccacagtc gtgtgtatgc ctatgtcgaa tatccagccg cgttaagaat
     3421 cgactcaagc acatctaaat tccttttgta tcagatagca ccttcaatta aaaaattcat
     3481 tttagcggat gataaaaggc gcaatggcca atgcaatttt gataatgaaa ccatggcttt
     3541 gccggattta atgcctcttt taaataaaaa taatgaggtg attgacaaaa aaagagcaaa
     3601 tcgtttgcgc tttatgctca atgaggcctt tagtgagggc gatttccaga gtattaaaca
     3661 ctttcaagac aacatgcgcc ttttaagaga tgaggatttt cagccaattt caaagattaa
     3721 ttttaataac ctcaccggca taagcaagca aatccttgtt gtttcaaagg ggtgaagaga
     3781 tttcttttct taaataaagt ttggccataa agccttcatt ttgataacca agattcttgt
     3841 aaaagagatg tgtgccctct tgagcgcgcc tcagcccgga tgttagatca atcacacttg
     3901 gtgcgctttg agaggcgata ttttccacga actgcataag cttttgaccg actccccttc
     3961 gccggtaagc gtgacccacg acgaggccct caatccgaaa ttttactttg cctgaaacaa
     4021 aacaaggcgt ttttgaccat gcaatccatc ccacaaccgt actttcagtc acagccacac
     4081 aaagaccgca atcctccttt tgagtgaaaa tgttgaatct gtgttcaaga tcttttgcct
     4141 caacggggta ccccaattga tcaaaaaggg ggatcatgtg cgggatgtca tcagcgatag
     4201 cataacggag ggagagagac ataggaggtt gaggctattg tttacaattt tatcaaaata
     4261 aatgcataaa acgagaaccc cttgcaatat tagatttttt agtctatatt tagattaatc
     4321 agtctaaata tttttggccc ctgcacatgt cgatctatgc acctcttgcg aaaagtatcg
     4381 cgcaactttt ttctcctttt gtggaagttg tgattcatga tcttgagagc ggcaagctat
     4441 caaaatcccc accccatcat ttgtggtgtt gtgatcgatc cgtgaatctg taaaaataaa
     4501 ttaaaataat gtcttcataa aaaaaacttg tgccagcgca tcttccagaa aatgataaac
     4561 acctatcgct taaactgaca acacctcttg gtccgcaaaa actgatggtt gacaaagtgc
     4621 atatgttaga gcggctgagt gatgtttatc atctgacgat tcacgcccat ggcaaggaaa
     4681 gtgatattga ttttgaaaaa ttaatgggta aatcagcgca acttcttttg acctatgatg
     4741 gtgataaaaa gcgctacttc acaggaatca ttggtgaagt taggcaaggt gtgaccgaag
     4801 atgcgtcggg tttttcacac tacatattga cactttatcc aacattttgg atggcgcgct
     4861 ttaaccagga ctatcgtatt tttcaaaata aatccgccaa tgacatgtgt gaggaattgc
     4921 ttaaagagta taatgtgccg gatactcgct ataatgcttc taaaaagggc tcgaaaaagc
     4981 gaaaatattg cacacaatat ggcgagagtg cttttgattt tattaaccgt ctatggtcgg
     5041 aagagggtat tttttatttt ttttcccacg cctcttcagg cgatacgctt gaggtacacg
     5101 atgctgtttc ggggcttaaa ccccttgaca aagagactct tgagtttacg cctcaacagg
     5161 tgggctctgt atcctggcaa aatgctgttc aagaatttta taaagaacag cgtgtagtga
     5221 gcaaaagtgc gtcccttgcg gattatgatt ttacagcacc ggatacaaag ctttatcaaa
     5281 aaacttcggg caagggtgaa ggtggtgaag tgtatcgcta tcccggtcgt ttttttaaaa
     5341 tggagacagg tgaagatctc aataaaacgc atatggagca gctcgagtgg aatcgtcata
     5401 tgttttatgg tcgcagcaca gcgccaggaa tggcaacggg ggggaaattt aagcttaaaa
     5461 accatccaag aaatgatacc aatcaggatt atattgttta tgaggttgaa catttttttg
     5521 atgtacggga gaatgtcaag aatgagagtg accgtgccct ttattacaat cggtttacgt
     5581 gttttccagc taaaacacct tttcggcctt ttcctaagcc caaaccgcgt atttacagca
     5641 atcaggtcgc cattgttgta gggccttccg gcgaggaaat tcacaccgac gagcatggtc
     5701 gtattaaagt gcaattttat tgggatcaac gtggtaaaaa taatgagaaa agctcttgtt
     5761 gggtccgtgt ggcccacatg tggggcgggt ctggtttcgg cacaatgttt attccgcgca
     5821 ttgatatgga agtggttgtg agctttttag agggcgaccc agatcgtcct cttgtggtgg
     5881 gatccgttta caatgggaat aataaacctc cttatccctt gcccgatgag aaaacaaaaa
     5941 gcacgattaa aacgcaaagc tcaaaaaatg cgtctgatgc gtataacgaa atatgttttg
     6001 aggataaaaa agatagcgag caactctatg tccgtgctca aaaagattat gatcgtgagg
     6061 tcatgaaggg tgaccgtacg ctgattattc acgagggaga tgacacactt acgattaaaa
     6121 aaggaaatcg cactcttaag gttgatacgg gggatgaggt gattaaaatt gcgggtacgc
     6181 gtactcttaa tgtgacgaaa acagaaaaac acactgtgac agatgatttt acattggatg
     6241 tgagtaatca taaaataacg atcaaagctc ttaatattga tattgaggcg ggtaacacga
     6301 ttaatataaa agcgacaaat gctgtgaata tcgaaggcac agcttctgtg aatattaagg
     6361 gggggaaagt gggtgtggat gccatgacag aaattgcaca taatgccaca acggaatata
     6421 aaattgatgc ccttaaatac acactgaatg ctaaaacaat ggcggatatg acagcatcta
     6481 tgatgaaact cacggcgaat gctcaaacaa ctatgatgct tggccctgtt gcaaatatca
     6541 attgctcggg tgccctttcc atcaatagct caggtgtttg tgcgattgtt gcctcaggtc
     6601 ctgccgcctt taaaggggct gtgacaaaac ttggttaggg atgtttgtaa tcaataaaag
     6661 aaatggcaaa aaataatgac ccgtgaagtg attcacaaag atgatcaagg acgcataaca
     6721 gcacgactct tctttgacga gcaaaatcag ctgcatggag tttgtgaatt ttatgaacaa
     6781 gggaaacttt tttttaaggg tgaatatgtt catggaaaac tacacggtcc cgcctcaacc
     6841 tatcatttta atggtcagat ccaatcaacg atgtatcatc atgagggaca attgcatggt
     6901 gagcgtgttc actatagtga ggaaggtatg atcactcaaa tcgataccta tgacaaaggg
     6961 ttgcttcatg gtccatttaa atcctttcat agtaatggcg ctgtggcatc agaaggcacc
     7021 tatgaccatc acattaaagc aaatgattgg gttcactatt atgataatgg tgaggaaatt
     7081 atcacagaag acgaatctga gtaaaatatc ttttgcttct cctaaccttt ttttaatctt
     7141 ttgagcttag cctaaaggca ttggtgtatt atttttttta agtacattga aatatttttt
     7201 gatcttatgt ctctttttga gtaccaaccc tctctttgct gggaaagagg gggataaggc
     7261 tgatcatggt gaagcttctg agcatggagg tggtgaagga ggtgaagaca gcaaaaataa
     7321 agttaagccc tatatcacca ttgggcctct tacgattagc attgtcaaag cagatgaaat
     7381 taaaggctat ttgagattca caattaattt atggacccaa acagaaaaaa acttcgaata
     7441 tgtacgctcc cttgccccct atttacgcag tgaatacatt atacagttgt caagtatttt
     7501 gagtaatttt tggatcacag gatcagaacc gcgccttgat aatattaaaa atattcttca
     7561 gcaaattacc aacaaaattc tagggcccgg taaggcacag gctgttctct ttgataattt
     7621 cttttttgca cgcccgcctg aaaacttaag ggagcctttt gcagccagcg aacagaagaa
     7681 tatatctacg ccgcagatct caccaaaagc ttcttcgagt aatgctgcag aagctcatta
     7741 aggcgacttt catcgcaatc atccgcagct tttaaagcaa cgtcaagatc ttcattttct
     7801 gtccatgccg tatgctcact tgtccaaata tagggggatt ttgtcgcttt gagtttgatc
     7861 atatttttag gttgtttttc ttttgtactt ctaaagacta tgggaaggtt ttgagaaatt
     7921 tcgtcttttt caagggacaa catatagtgg gctaagtcga gcgtgcacac tttgtgattg
     7981 gttgtgagtg tatattccaa aaggtctctg gagtttttaa aagcggccag tgttgaaaca
     8041 atgagcgcac tcatttgttg agctgttatg agagaggttt cagcgccctc gtgatcaata
     8101 tggatgtgta gaccgtcatt aatgtgagat tgaagcgatg ctgttataga ggccgagctg
     8161 ccaataatat tgtgaatatt tgcaatgaaa tactgtgtgc ttttttcata aaaatagcgc
     8221 atgaaggccc ctttaataag gctttcaaac attttgagtg atgtactctc aaaaagatca
     8281 ttcggataaa aatctgtggg attttcaaaa agaagcaagc attgttcgac tttgtggtgg
     8341 tgtgcaagat aagagagaaa ctctgttgca aggatattgg tgcgaaaata ggccagctga
     8401 ttttcctcac caggccaggt cataaggggt gtgtgaatca taatatgagg cttgtgattt
     8461 gcaataatag agctgatgag aggttgatca ttaatatctg caacttttaa aacaaaacgt
     8521 cctcggtcat agttttgact ttcaagccaa atacttagtt ttttaagacg ctccatcgat
     8581 tggtcaacaa ggattaattt tttagcatcg agatcgagga aaagttggat gagcggtcta
     8641 aaaatataac ttcccgcacc gatgatcaga atttttttag tgttgatatg cgctttttcc
     8701 tcctttgaac agctcataac aggctcttta aaaagagact ccacagaaag ttctttaagc
     8761 tctgtatttt caaaggtaaa ggcttttggc atacgaagaa gctgaatacg attctcgtta
     8821 gcaatccgca aaatatcatt caaatatttt ccaggatgtt gaatgatcac aagacggagt
     8881 gtttgcgcta aaagcccagg ctgtgttgta agccaccttg agagatcttt tacacttgtg
     8941 acaacgggaa taccatgaat actcaggcca atttcatcgg accgaagaga aataagtgca
     9001 tagggcgtat agttaaaatc ctcattttga ctcatttctt ttaaaaacaa ttcactacga
     9061 ttatcggtgc caagaataag gagaggcggg cgcttgagct tcagcctttg ccgcttgtga
     9121 tgactaaaat agatgagaag ccgtggcaca aatgtcatca ttaaaaaaac aacccaggca
     9181 atcaaaagtg taaagtgggg catttgttct tcgcggctca gaaggtgttt gagcggaata
     9241 aaggtgaga
//
LOCUS       DAIAHG010000029         9215 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_29_length_9215_cov_3.798862, whole genome shotgun sequence.
ACCESSION   DAIAHG010000029 DAIAHG010000000
VERSION     DAIAHG010000029.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29_length_9215_cov_3.798862"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2487 a         1905 c         2163 g         2660 t
ORIGIN      
        1 tcgaagaatt cgaggtcatg gcgcgcgata caaaactggg caacgaagaa atcacacgcg
       61 atattcccaa tgtcggcgat gaggctttga gcaatttgga cgaagcaggc attgtttatg
      121 tgggtgcaga agtagctgcg ggtgatgttc ttgtgggtaa agtcaccccc aaaggcgaag
      181 ttcccatgac gcctgaggaa aaattgctgc gggcgatttt tggcgagaag gctgcggatg
      241 taaaggattc ctccttgcgc gttcctcctg gcgtgagtgg tactgttgtt gaggttcgtg
      301 tgttttcaag gcgcggtgtg gacaaagatg aacgtgcgat tgcgattgag cgtgcagaaa
      361 ttgaccgcct ttccaaggaa cgcgatgcag agcgtgctat tttagaggga agtttctttc
      421 aacgtctcga ggaaaaactg attggccaag agcttataag cgcacctaaa gacattaaaa
      481 caggtgtgaa aattacaaag caactgattg attcttgttc acggatgcaa cttcgtcaac
      541 ttggtgttgc caatgataaa gtgatgcgtg atattgatag cctcaaagct gaaattgacg
      601 ccagcattgc aaaacttcag gcaaagtttg aaaataaagt cgataaactc cgtcgtggtg
      661 atgagcttcc acccggggta ttaaaaatgg taaaggtgtt tattgccgta aagcgtaaac
      721 ttcaacccgg cgataaaatg gcggggcgcc acgggaacaa aggtgttgtg tcacggattg
      781 taccgcttga agacatgcct caccttgagg atggaacgcc gatggatatt attctcaatc
      841 cactgggtgt gccatcacgt atgaacgtgg ggcaaatttt ggaaactcat cttggcgctg
      901 cggcgtataa aatgggcgag cagatccagg gtgccgttcg cgcgtataaa gcaaacacag
      961 caacaattga tgatgtacgc agcaaactta aagatattta tgtatcgaaa gacgatcagg
     1021 ccgatcttca aaaaatgtcc gatgatgaag tcctggagat gtcgcacaat ctcttcaaag
     1081 gcgtgccgat ggcaacccct gtctttgatg gtgcacgaga aaatgatatt aatgagcact
     1141 tagttcagtc aggatttgca caatccggtc aggtcacact cattgatggt cgcacggggg
     1201 aagcgtttga gcgtcctgta acggtggggc aaatttatgt tctaaaactc catcacttgg
     1261 tggatgataa gattcacgca cgctctattg ggccttacag tcttgtgacc caacaaccgc
     1321 ttgggggtaa agcacagttc ggtggtcagc gctttggaga aatggaagta tgggcgcttg
     1381 aagcgtatgg tgcggcttat acccttcaag aaatgctaac gataaaatca gacgacgtgg
     1441 cgggccgtac aaaagcgtac gaatccattg tgcgcggtga tgataatatg gaagcgggta
     1501 ttcctgaatc ctttaacgtt cttgtaaaag agcttcgctc tcttgcattg aatatgtcct
     1561 ttgagcaaaa gtattaagac cctgggagag aaataatgac aaagcaactg attcaaagag
     1621 cgacaacaag cgcagaaatc attcctggtt ttgatgcttt aaaaatttcc attgccagca
     1681 gcgagcggat tcgctcctgg tcctatggcg aggttaaaaa acctgaaacc attaattatc
     1741 gtacgttcaa accagaacgc gatggtcttt tctgtgcgcg tatttttggt cctgtgaagg
     1801 actatgagtg tctgtgcgga aaatataaac gcatgaaata ccgcggtgtg atttgtgaaa
     1861 aatgcggtgt ggaagtcact cttagtaagg ttcgccgtga tcgcatgggg catatcgagc
     1921 tggcctcgcc tgtggcgcac atttggttta caaagtcact gccaagccgt attggtatgc
     1981 tgcttgatat tactcttaaa gatttagagc gcgttcttta tttcgagtct tatattatta
     2041 ttgaccccgg tatgacgacg tttaaggaaa agcaactcgt taccgaggaa gaatatgttc
     2101 gcgcccaaga tgaatttggt gaagatgcct ttacagccgg gatcggtgca gaggctttaa
     2161 aagtactctt gcaaaaaatt aatccagaag cagagcgcat tgagctgcgt gaggatttat
     2221 tgcaaacaac ctcggaaatg cgccgtaaaa aactttctaa acgcctcaaa ttgattgaag
     2281 catttataga gtctgataca aagccagagt ggttgattat ggacgttgtg ccagtcattc
     2341 caccagagct tcgccccctt gttcctttgg atggagggcg ttttgccaca tctgatttga
     2401 acgatcttta tcgccgtgtg attaaccgta ataatcgtct taagcgactg attgaacttc
     2461 gtgcacctga aattatcgtg cgaaatgaaa agcgtatgct gcaagaatct gtggatgcac
     2521 tgtttgataa cgggcgtcgt gggcgtgcga ttgtgggagc gaataagcgc cctcttaaat
     2581 ccttggcaga tatgctcaaa ggtaaacagg gacgattccg tcaaaacctt cttggaaagc
     2641 gcgtggatta ctccgggcgc tctgtgatcg ttgtcggtcc tgagcttaaa ctgcatcaat
     2701 gtggtttgcc aaagaaaatg gcgctggagc tttttaagcc ttttgtgtat tcacgcctcg
     2761 agcgttatgg ccttgcaagc acactcaaag ccgctaaacg catggtggaa aaggagcgcc
     2821 ccgaagtatg ggatattcta gaagaggtca ttcgcgagca tcctgttatg ttgaaccgtg
     2881 caccgacact gcacagactg ggtattcagg cgtttgagcc tattcttatt gaaggcaaag
     2941 cgattcaatt gcacccgctt gtgtgtgctg cttttaacgc agactttgac ggtgaccaaa
     3001 tggctgttca cgtgccacta tcccttgagg cgcaactcga atcccgtgtg ctgatgatgt
     3061 catcaaataa cattttgaac ccagcaaacg gcagtcctat tatcgttccg tcaaaggata
     3121 ttgtccttgg gctgtatcac ttatctctcc tgcgtgaggg aatggcgggc gaggggatgg
     3181 tgttttcgaa tgtcgccgaa attgagcatg cgctttttga gaaaacagta caccttcatt
     3241 ctaaaatttg gctgcgtctt gatttgatgc aagaagatgg cgtgatgaaa cgtatgcgcg
     3301 tcgaaacaac accagggcgc gcgattttat ggcaggttgt accttgcaac cgcgatattc
     3361 cctttgcact ggtgaatcag acgctgagct ccaaagaaat tcaaaacctt gtcgaagcgg
     3421 tttatcgcta ttctggtcaa aaggatacgg tgatttttgt tgataaaatc atgggccttg
     3481 gcttccgcta tgcaacagag agtggtattt cctttggtaa agacgatttg attattcctg
     3541 aggaaaagaa aaaactgatt tcggatacaa aagattttgt ttctgaatat gaacaacaat
     3601 atttggatgg tttgatcaca cagggtgaaa aatataacaa ggttgtggat gcctggtccc
     3661 agtgttcaga taaagtcgca gatgcaatga tgtcggcgat gtcaaaagca gcaccaggtg
     3721 aaccattgaa cgccgtttat atgatggccc attcaaaggc gcgtggttcg gcggctcaaa
     3781 tgaagcagct tgcaggtatg cgtggtctga tggcaaaacc atcgggggat attcttgaga
     3841 cgccgattat ctcgaacttt aaagaagggt tgaacgttct tgagtacttt atttccaccc
     3901 atggtacgcg caaaggactt tcagatacgg cgcttaaaac ggcaaactcc ggttatctta
     3961 cgcgccgtct tgtggacgtg gctcaagatt gtattattac agaagtcgat tgtggcacgg
     4021 aaaagggtat attaatgcgt gctgtgatcg aaagtggcga tattattgcc tcccttggtg
     4081 agcgtattct tggtcgtacg gcggctgtgg atattattca ctcacatacg ggtgaggtga
     4141 ttgtgcctgc tggaaaactc attgaagaag acgaggttga gacgattcat aacgccggcg
     4201 ttgatatggt gatgatccgc tctgtgctga cgtgtcaaac gcattttggg ctttgttcta
     4261 agtgctatgg acgcgatctt gcgcggggta caccggcgaa tatgggcgag gctgtgggcg
     4321 tcattgctgc acagtccatt ggtgagcctg gaacacagct aaccatgcga accttccaca
     4381 ttggtggtgc tgcaaaagtg ggaacggagc aatcaagtat tgaatccgcc tatgcaggaa
     4441 aaattacaat taaaaacccg aatatcgtca ccaatagcca aggcgtatcc atcgttattt
     4501 cccgtatttc tgaggttgtg atcgtggatg agcgtgaccg tgaaaaagtg cgccaccgcc
     4561 ttccttatgg tgcgcgtctt ttagtgaaag agggggatgc ggttgctcct ggtcaaaagc
     4621 ttgtggagtg ggatccttat acgattcctg tgatttctga aaaagaaggt attgttcact
     4681 tcgttgacct tacagaggga ctttccatgc gcgaggtcgt ggacgaggca acaggtatct
     4741 ccagtcgtgt ggttgtggat tggaaacagc aagcacgcag ttctgatctt aggccgatga
     4801 tttcgattcg cgataaaaaa ggcaatcccg ttattctcca aaatggtctc gaagcacgtt
     4861 acttcttgcc tgtggatgct gttctttctg tggaaaatgg tggtgtgatt aaagcgggtg
     4921 atattattgc ccgtgtgcct caagaaacag ccaaaaaccg cgatattacg gggggtttgc
     4981 cacgtgtgtc tgagcttttt gaagcgcgtc aacctaaaga tgctgcgatt attgcagagt
     5041 gcgatggccg tattgagtac ggtaaagatt ataaaatgaa acgccgtatt atgctggtgc
     5101 caatggatga aaaccaacct cctattgagt acttggtccc caaaggacgt cacgtgacag
     5161 ttcacgaggg ggatatggtt aaacgtggtg atattcttgt ggatgggaat cctgtgccac
     5221 acgatatttt acgtattttg ggtgtagaag ccttggcaag ttatcttgtg agtgaaatcc
     5281 aacaggttta tcgtctccag ggtattaaaa ttaatgataa acacattgaa gtgattgtcc
     5341 gtcagatgtt acagaaactc gaggttgtgg atccaggtga gacaacactt cttcttggag
     5401 agcaagttga tcgtgcagag tttgaggcga tcaataaagc agctgacatt gaaggacgtc
     5461 gtctggcgcg tgcgataccc gttcttcaag gtattacgaa agcatcgctg cagacaaaat
     5521 cctttatctc tgccgcatcc ttccaagaaa caacgcgcgt tctgaccgaa gcagcgattt
     5581 ctggcaaaat ggatgatctc gctgggctca aggaaaatgt gatcgttggg cgtcttattc
     5641 ctgcgggaac gggcagtgta atctcacgcc ttcgtgctat tgcgcataat cgtgatgatg
     5701 attcgcttac ggtgcaagcc catgaaggac atagtgcttt atcttccaat gatgagattg
     5761 ctttattgtc ttagacctcg caaagctacc tcctttttta aggaagtagc tttatttctt
     5821 tgatattaat ttctcctgaa aaacaagact aactaaacga gatacttccg aggatatcac
     5881 ttaaaaaagg gggaggtgaa tccccccctt ttaaaatgat gaaagtaatt ctttttcttt
     5941 aaaatttcat aatacttccc tagcgtttct ttagtttttt ttcttaaaaa tgttcataag
     6001 tttttcaatg gatgccttgt acccgctata gtatttttta tcataaccgc cacaggtttc
     6061 ctcttgcttc tcaaacccca gtgtttcata ggatttgatg gctttttcat tcacatgacg
     6121 tacgtcaaga gaaagtgttt ttatcccctc ataaagattt ttcgataaaa tggccaaaag
     6181 agcggttttc aaaagccctt tgttttgatg attctttatc aaaaagccct gactcccgta
     6241 aagatcgtgt gtctctttgt tataaagagc ggttccaaaa cctataaagt taccctcttt
     6301 actgagcaaa taaaaaagat cgatctttcc ctctttaaat tcatcaatct ctccattgat
     6361 tgcagcgtta agccacgctt ctttgctgtc aaagatacct tttttaccaa gatcttcgag
     6421 ggaatatttt tcataaacct ctgaaaacac agtgacaaat tcttttttaa tgctattttc
     6481 gatttctttt ggaagttcct cccttttttc aggatggtag attgtgtaaa cattgttatc
     6541 ttttgacgta atctcaatag gatcaaagac cgcaaagaca ctattataac ctccaaaagc
     6601 caagaggata gttaaactta aaacctttaa acattttaaa aacatagaac ttaatctcct
     6661 ttttattcgt ttttgaatgt ctgtgtcaca attgctgacc gcgccaaatc gtctgcacgt
     6721 tcgttataca cattacccgc atgacctttg acccagtgcc aggtcacctt atgctgatcc
     6781 tttagttggt ccaacgcttg ccacagatct ttatttttaa caggtgtttt agaggctgtc
     6841 atccagccat tttttttcca tcctttgatc catgtattca gcccatctct cacataggtg
     6901 ctatccgtgt agagatccac ttgacacggt ttaggcagga ttttaagcgc ttcaatcgcg
     6961 gcgatcattt ccatacgatt attcgtcgtg gcacgttctc ctccaaagat atcttcggat
     7021 aaatcattaa aaagtaaaac agccccccag ccccctggcc caggatttcc actacacgct
     7081 ccatctgtgt aaatcacaac gtggggcaaa tcctccaagg attttgaaat ggcctcgatc
     7141 actgaaggat gattgaagtg gttatgtaac gaatgcataa gataaagacg ctcataaaat
     7201 ttgaaatctg aggaagatta tacgctacgg attaacggtt ttgaaagatt tttcccagac
     7261 aatgttcccc caagaaaaaa atacttaccg aataacaaac acaattaaag attgtatttt
     7321 catagcgttt ccttagaata tcgcaagatt aaatttttaa aaaacataac catgacgcga
     7381 cttcgccact tttatcatat tttatccgag cacatttttc cgtggtggcg ctcagaagag
     7441 aaaaaaatcg catggggagg gatgattgca cttttggcca tgagcctcat ttctgtgtat
     7501 attgcggttg ttttaaacgc atggtcgcgg gattttttta acacgattga gcaaaaagac
     7561 ttgaatgctt ttctccatca agttttaatt ttcattccac ttattgcgat tgttcttttt
     7621 gatttttgtt cccgtaacta tcttacggcg tggctgagct ttcgttggcg ccggtggtcg
     7681 acggtgcgcc tacagacaga ttggcttgcc cataaaaatt tctatagaat tgcccttcag
     7741 accaaggaaa tggataaccc tgatcaacgt atttctcaag atataaattc tgtatgctat
     7801 gcctttattg gtatcgcgct gtcatttttc cgcgaaggtg tgaattttat taccttctcc
     7861 gcgattttat gggaactgtc gagtacgttt gatttcactt ttggtcgcta taattttaat
     7921 attccaggtc tcttggtgtg ggcgtcgctt ctttactctc tccttggcac tcttgtgaca
     7981 tttaaagtgg gtggtccgtt gatcgagctg gataggctgc aagaaaaacg cgaggcaaat
     8041 tttcgttatc gcttaatgcg cattttcgaa cgtcgtgagg aaatcgcaac actttcaggc
     8101 gaaaacgtcg agcataaagg tcttaatgcc acgtttaaag atattacaga aaattattat
     8161 aggattctga aaagacagat ttatattaac cttttccaaa acttttatat gaatgccagt
     8221 atgttcgttc cccttttcct tgtggggccg ctctatttta aaggagtgat cactatgggt
     8281 gttttgatgc aaattcgggg aatgtttagc gaagtcaata tgtcactttc ttcgattata
     8341 atggaatatc aaggcattgc atcggcagtg gcgtcactgc aacgtattat gacgtttaag
     8401 cagctcatga aaaatcatga aaagagtccc caccatctct tgccaattaa tagccatggt
     8461 atacacatcc cctcccttca cctccatacg ccaacgggca gtcccatttg gacatctccc
     8521 gcatttgatt tgaaagcagg agaccgaaaa attctaaaag ccccttctgg cacaggaaag
     8581 acgagtcttt tgcgtatgct ctcaggcatt catgttcacc aggatggaaa gcgactttcc
     8641 cttggcgatg aggccatgat tattccccaa agaccctata tgcccatagg aacccttaag
     8701 caatgtctga catatccttt ggatgaagac acgcataaca cagaaaccat tgaaaatctt
     8761 atgattcagt gtcagctaaa gcatcttatg cctttcttag acaaagtggc tgactatcaa
     8821 aatcgattgt cactgggcga gcaacaacgt attaattttg tgcgcgttct tttgaaaaaa
     8881 ccccagtggc ttcttatgga tgagcctttg agttccctta atcacgatta tgcagagatg
     8941 gtgatagcag cccttgatac atctttgcca aaggccggaa tcctcattgt atcccatcaa
     9001 gatattcaag gatttaggcc tgtctcctta tgagcggttt tttgacgatt ttatgcgttg
     9061 aaacggtctt tttcggcgaa aaaaacgcgc tgatcgctgc gttaaatggc aagacgcctt
     9121 aaattttctg caaagggcga aagatttttt cttttgatcg ctcttgaaaa aaacgaagtg
     9181 agcagctttt tctcaaatta tgagcctaaa acatt
//
LOCUS       DAIAHG010000030         9192 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_30_length_9192_cov_3.576742, whole genome shotgun sequence.
ACCESSION   DAIAHG010000030 DAIAHG010000000
VERSION     DAIAHG010000030.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30_length_9192_cov_3.576742"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2818 a         1829 c         1927 g         2618 t
ORIGIN      
        1 aaatcgctta aataatgtgc aaacgcgtat aaaaacgaaa aatttcgtgt caaaatagca
       61 aaattatccc gaaaatctat gcatcaggaa tctaacaggg ggcttcagta tttttaaaaa
      121 tactcacgcc tcttatagga aaagggggct tgtttaatga ttaaaatgta agctaaaatt
      181 atattaattt atgatgaaca cttcttaaaa caggcttttc tatgtcaaaa acacatcaca
      241 tcacactcat tcaaggggat ggtattggcc ctgaagttac aggggcaact cgcaaaatca
      301 ttgatgcaac aggtgtcaaa attaactggg acatatgcga agcgggggcg gctgtgttta
      361 aaaaaggctt agccacaggc cttcccaagg aaacgattga ctctattcac aaaaataaaa
      421 tcgcccttaa gggaccactc gaaacaccca ttgggtttgg tgagaaaagt gcaaatgtca
      481 ccttgcgcaa aatgtttgaa acttacggca atatacggcc tatccgggaa ttaccggggg
      541 taccaacacc cttttcaggg cgcaatattg accttgtgat cgtgcgtgaa aatgtagaag
      601 atctttacgc aggtattgag catatgcaaa gtcctggtgt ggctcagtgt cttaaactca
      661 tttcgcgcaa agggtctgag aaaattattc gccttgcctt tgaatttgtg cgaagtgaag
      721 gccgaaaaaa acttcattgt gctactaaag ccaatattat gaaattgacc gaggggacga
      781 tgaaacgggc ttttgaagat attgcaaaag aatatcctga cattgaggcg catcatatta
      841 ttgtggataa ttgcgcgcat atgatggcaa agaatcctga gatgttcgat gttattgtga
      901 caacgaatat gaatggtgat attttaagcg atttaggctc agcacttatc ggtgggcttg
      961 gctttgcccc cggtgcgaat attggagatg atgtggcgat tttcgaggcc gttcacggct
     1021 ctgcgcctaa atatgctgga aaaaatgaaa ttaatccaac ggctgtgctg ctttcaggtg
     1081 tgatgatgct tcgtcatatt ggtgagtttg acgcagcacg gatgattgag caggcgcttt
     1141 atctcacctt ctcaagtggt tgcattacac gggatgtcaa ggtgccgggc aaaaatcctg
     1201 tcagcacgac agcttttacg gacgaggtga ttaaaaacct tggtaaggag tatgatcatg
     1261 aaaaaattcg cccttataaa ccaattgcaa aagtggctat ctctcaggat gtggattttg
     1321 tcaaaacgaa gaaacgtacc gttgtcggtg ccgatatttt tattgaaacg acaaaaccta
     1381 tggcggcttt tggtcctgag atggagagct ttggccataa acatcatctt accctcaaac
     1441 tgattacatg ccgcggtgtc aaagtttttc cgagggatca tgagatttct ccagacgtta
     1501 ttgatgttgt gcgctgccgt tttatggcac ctcaagagac ggagcttgag cataaaacac
     1561 ttcatgcgtt gttagccgat atagagtccc actatcactg gatgcatatt gagaagctcc
     1621 aagttttcga cggtgttata ggctattcaa aggctcacgg agaagattag aatttctaga
     1681 tttattctgg cacgcttttt gcactttacc tgtaacagag agtgcaattg ggcgtgtttt
     1741 tcgtgttttt tttacgtctt ttaatccttt tttcttttgt tttttctaac atccatccaa
     1801 cactggcaga tgtccgtgtg cgcatcaaag atattgtgtc ggtggaaggc gtgcgtggca
     1861 accaacttgt gggttatggc cttgtagtcg gcttgaatgg atcgggggat acgattggtt
     1921 cgacgacatt tacgcgggaa agccttgcaa gcatgcttga gcgtcttggc gttaacgtgc
     1981 gtgataacac aggggctgct ttgctcaatg gtaaaaatgt agccgccgta atggtcacag
     2041 caacccttcc tccctttgca cgacaggggt ctaagatcga tgtcaatgtg tctgctcttg
     2101 gtgatgcaaa ggatttacgg ggtggaaccc ttttggtcac acctcttctt gcagcagatg
     2161 gacaagttta tgctgtggga cagggttctg tcgctgtggg gggatttaaa gcagaggggc
     2221 aggcggcttc tgtaacgaaa ggtgtaccca catcagggcg tatttcatcg ggtgcaattg
     2281 tggaaaaaga aattggcttt gagctagcgt cgatgaatca cctgaaattt aatcttaata
     2341 atcctgattt tacaacggca aagcgcattg cggaccgtat taatcatact cttgggaatg
     2401 gtgttgcaaa agcagaagat ccctccacag tgcaaacgtc tatcccagat agttatcgtg
     2461 gccgtaccgt ggaatttatg acccttgtgg agcaattaga aatcgagcct gatcaagtgg
     2521 caaaaattgt gattgatgac catgatggtg tgattgtcat ggggcaacac gtgcgtatct
     2581 cccctgtggc gatttctcat ggaagtatca caatcaaagt tacagaaaat cctgaggcta
     2641 ttcagcctac aaatattgta caggccaata caacggcttt aaatgccaat gcaacttttg
     2701 gcattaacga tcaaaatcaa agcacaagtc agaccaatac ccccacaacg aatacggtca
     2761 caggtaatcc aataaccaca accacgaata cgacgacaaa taataatagc gctacgaata
     2821 acacaacaac aaatcaggcg gctcaaccca gcaatattac aaaaagcaac acagggatcc
     2881 ttaacgttgc acctgggtct caaaacagtg caacctttgg tccaggggga acgagctttg
     2941 ttgcacccgg cattgggacg gctgttgttg cacgcacaga tattaaagtc acagaagaaa
     3001 aaggaaaaat ggcagtatta gagtctggtg tcacgcttca gcaacttgtt gatggtctca
     3061 atgcccttgg agccacccca cgggatatga ttataattct ccaatccatc aaagcatcag
     3121 gtgcccttca agccgaaatc gaagtgatat aaaagaagtt cttttgctta aaacatagga
     3181 ttaaaaaggg gctagaattt ttcataggca tcctcttgac ttatgaaaaa attgctctac
     3241 ttaacaaaca aaagtgattt tttatcatta aaataggcag gcggttatgc gtgaacaaaa
     3301 tacaaaaaat aggaagcgct ttctttcgct ccttgtgctc ctttgccttg tatccactac
     3361 ccctgcagaa agtaaagagt ggtttggtgg agtcaaggac tatttttgga aaaaatccga
     3421 aacaaatgcc caaaaagctc ccccacctct cacaaaaacc gattcgtctt ctgagaaaac
     3481 gcaagatcat actgatcaag agttatcgac gaaagtgaca tttgaggcgg tgggtcaagg
     3541 gaaaaatgac aaaacacctt ctacagaatc atggggaaca tggtttttgg ataatggtgt
     3601 gcgctatggt gtgacgacgt tttcgaaata tgcgggaagt tatcttgggg ggcagttttt
     3661 ccaaaataat acaaaaaatc ttcttgatac gcgctatgac aaacttagct ggttgaaccc
     3721 ctgggaggct tatgaaaagg ccaatatatc tctgcaatat gcaaataatc tccaggagtc
     3781 ttatgactgg ggcactcatg tggggggcat tgttggaagt gttgcaggag gtgtttttta
     3841 tgatattgca aaaatcactg ggaataccct ttatgaaata tacacagaat atcagggaga
     3901 taaagagccc ccaaaagact caagatggtc cactgtgggt tgggatgtaa tgtcctctgt
     3961 tgcctcgagg agtgcagaga cagggcttac aaaatttgcc tcagatgctc tttataagtt
     4021 tgccaaaaac acggtgaaat attatgtgag agaaaagctt gctaatccag tcattacagg
     4081 tacactggga gcgattctcg gtgagaatat tttaaaaaaa gtcgcttctt ggcctctttt
     4141 agggtatgct ggagaagctg aggccttgga taaagtttct gaaggaattg ttaattttgg
     4201 catccaaggg gcctcttatt tcgggtcaaa ggttgtcgtt ggcggtggaa gttatcttgc
     4261 ccaatcaatt tcaaactggt actggggtag tggtgaaaat atccctcaag aagtagctcc
     4321 aaagattaat ttagacaagg ttattgataa aaaattagaa ctctcagagt taaagcaagc
     4381 ccctccacct cccttgaata gcaacgaaaa tctttatgaa tccatagcag cagcataatc
     4441 attcaagagg ggcttgcaga ggtatttcat cgcggtgggt atcaataagc tcttcgaaca
     4501 atgtgtgcca attaagttct gcgcctaaat gaaacaaaac agatccaatg cctacagcgg
     4561 ctctatccat aaaaacaaat tctcttggag gcttcacacc gccacactga ctaaggcgac
     4621 tatggacatc ggatgccaca tcccatcctg ttattttttc ttgaataggg cgaatacgat
     4681 catccaaaag aggatcaaac aaaagctttg cccaatcact cataatctcc atgacctcaa
     4741 aggataaatg atcaaacccc cacatctcat acgcataggc catacggtca cgatcatttt
     4801 ttttaagtgc ctcgtacaaa tgaatcacgc cttttataaa atttttatta aaatgccgca
     4861 cacaaccaaa atctaaaaga caaagcccct gctttccatc cacggacgtc actctataat
     4921 tgccaggatg ggggtctcca tgaataacat tgtggtgata aaagggaaaa taccacccat
     4981 gaaaaagaag tcttgccaga tgattgcgct cactaagaga gctctctgta aaacgcatca
     5041 aagattcacc ttccacaaaa tccatcgtta aaaggcgctt tgtgctataa tttgtgaaaa
     5101 ctttagggac agaaataaga gggtgatccg caaaaatttt tccgtaggtt tgaagcgcct
     5161 ctgcctctaa aaaataatcc agctcctcaa agagtttttc tttaatttcc tcaaaaatat
     5221 tttctgtttg aatacccttt tgaaaggctt catagacacc taacacaagg cgaagctgtt
     5281 gcaggtctgc ctctacaatc gtttccatat cgggatattg cagtttgcac gcaacagtag
     5341 aaccatcctt caaagtggcc ttatgcactt gccccaaaga tgctgcaaag gacggctgaa
     5401 gatcaaaatc ttgaaaagaa tccggccaat tctcaccaag ttcaccacgc atacgacgtc
     5461 tgacaaaact ttctcccatg gctggcgcgt ttgtctgcag tgttagaaat ttttccgcgt
     5521 aggctttggg aagggctcct ggaacggttg ctaagaattg cgcgaccttc atcaacggcc
     5581 ccttgaggtt tccaagcgtc agcataaggg cttcagcttg tttttcctca tcaaaggata
     5641 gatttaaaaa atgttcagac gcgagtcgtg tcgctgttcc cacaagggat gatgatgttt
     5701 ttgcatagcg cgaaagcctt gtgaaaaaag gaaaatgatc atccatatga atcgacactt
     5761 aatgaatttt tccatcatac tatcacaacg caataaagcc tgtgactaaa attcactgtc
     5821 cttttcttct tactcctcag tattatgagg ggtaaagtcc tccggataca gcagtgcaag
     5881 catataaaaa atagagggat agagctttct tatctgcacc aatgttgcat catttacatc
     5941 atgaagttcg ccaagatcaa aagagttatt gtcataaaac tctttggcgg tttggagagc
     6001 ctcttcatcc ccaagggttt ttgtccaaaa ataggtcatg cgtggatcaa taacagcgct
     6061 attttcctta aagaaattga gaaatatcgt ttttaaagac tccgcctctt tttcaaaata
     6121 gatatactca tgttctttgg ctttttgttt tttggctttt tcaaggatag tttcaaaggg
     6181 caacgcctct atttcatgaa aatctttctc tccataaact atcccccaaa gatcattgat
     6241 cagggtatct gagtccaact cttcacaaat cgttgacata agagtgtttc tgttatcaaa
     6301 acgagcactc gctttctccc aaccattctc aacaagttcg attgtttttg ggtgaaatga
     6361 tctcagatga tcactccagc gtgcaagaaa tattttcgct tcatttacaa taaggtccgg
     6421 tgaaaaaaga ttgtagagaa ctttttctgg agtttcatgg aattttcttt ttttactatt
     6481 aatttcttta ttgaaatcat tttccttggc caatccttta tgaaggtgtg ttgaaaaaat
     6541 taaaaatccg agaaaaaaga aaaataattt catgtcattt caaaaattta attagagctt
     6601 ctcgaaaata ttttcgcata gtgggcacag aactgtccag gacacttaac ccatctaatt
     6661 ttttcctcaa gcgatttgcc ggatctcctt ttttctgtta gaggtaatgg agctcaagaa
     6721 aaagaaagtt tttgaaaatg ttttgtctta ttttgggatg ttttgccgcg cttttttcat
     6781 caacacttct tgctgtcagc gtgcctcaag aggaaactct acagcaaaaa tctcttgata
     6841 ttttcccctc tatgccaggg agagtcatta aaatatttgt aaaacctggg gataaagtgc
     6901 aagaaaagac ccgtcttttc aagatagatt cgataaaaat ggagatacct atatatgccc
     6961 ccaaggaggg gatcattgga gaaatttatt gtcatgcaga agacactgtg agcatggaaa
     7021 ccagtattct tcagcttttg gatcgtcata agatcgttga caaacctgtt gaaacattgc
     7081 ccatcagcct cccgcaagag cttcttaatc atagcaatag tgctccggat gagcttgaaa
     7141 tttcaaaaac tcaagccccc actcaagtgc tggagctgaa aaaagacaca gcatccgatc
     7201 ataaaaagag gacgtttctt cagccttcaa aaaaattatt tgcaactact gtggataaaa
     7261 atgaacattt tgaaacacct ccttgcccta cacaagaaaa agcaccgaac ctgccgatcg
     7321 ctctgaaaaa ctcccataag tttagtgata cggttgagca gatttttaaa aaaacacttt
     7381 atacaccgtt tgagctggaa aaattagagc cctatgctct gaggatttct catcaaacat
     7441 ttctctctgt tcacgcgtgg gcaagtgcga gtatgcccga agaaatttat cgtttttcca
     7501 cacgacacac acggcagatc actcataatc tttttataaa actacgggtg ggaccacact
     7561 ttttcaaaag ttttttacat tttacgaaga atctgcagga cgaggaaaaa ataaaatcgt
     7621 ggatctttag tcatcttttg ccagagcccg tggtaaattt tgcacacgta tggcctaaaa
     7681 atatttggac agcaaaagag tggcgactca tgatagaaaa tcttttgggc tttttgctga
     7741 tcggtctttt ttcgatcttc tttacaagga tcctcaaacc tttctctcaa cttgaaagac
     7801 aaatcattta ggataagttt ttaaacaaga tacagaaaaa aatattgaga gtttttcgca
     7861 aaatacgcta tagcaataat cagtacttgg actaaaagaa ggaaaaaaca atgacaaatc
     7921 accttggaat agcagcacaa caactgcgta tgtttattga aaaagtggag cgccttgaag
     7981 aggaaaaaag tcggatcatg gaggatataa aagatgtttt cgcagaggcc aagggaagtg
     8041 gctttgaccc aaaagtaatg aaacaaatta ttaagctgcg caaaatgaaa gacaatgagg
     8101 ttgcagagca ggaggaactt ctcgatcttt ataagcaagc ccttggcatg attccctcca
     8161 cgcatgcgct tgatgaggtt gcttaacaaa aagtggagat taaatgtgac tatgatctct
     8221 caaaaaaaga gcacgaggag cttttagagc ttttgatgga aagctttccg gggtatttta
     8281 aagatcgcct ttactttaaa aatattccgg cttttcgcgt acttgagcgt gtaaaggagc
     8341 gcgttattgc ccatgccggc gttgattacc gtgtgattca ctctcaaaaa aatggccccc
     8401 tctctatctt tggcattgtt gatctttgtg tttgtaagga tcaccagaac aaaggtattg
     8461 caggaaaaat tatttcgttt atcaaagatc tagcacagaa aaaatcgatt gatgcacttg
     8521 ttctttttgc ggatgatcat cccttttacc tcaaacatgg ctttgaaaat aattctgtta
     8581 cgtgtcgatt tcttgcgata gaagatctta gttctgtgtg tgttttggaa aaagatatga
     8641 gcgattgctt tatgataaga tcacttcaca caaacctttc ttttcaaaac gatcacattg
     8701 atatgcttgg gcctgtgttt taaaaaaatc tatcttgaag agacgtcgat ttatttttag
     8761 agtgagtcta ggttattttt tgtattattt tttcaatgat caaaattttt ttagtttgtt
     8821 taaatttttg catactcatt tctgaaagtt cccttgctcg agacactcaa tctccactcg
     8881 agaaaaagca tcaaaaaaaa ctttataatc agcaaaatcg tataaacagt aaaagaacaa
     8941 agcaggctat tcaaaaaaaa ttcaaaaaaa atgttaggaa gagtgttaga ataaacacaa
     9001 aaaatagagc tcattgatga caccccatat cactcttctt cgacaaaaac tcacagcctt
     9061 caatctagat ggctggcttc ttcctcactc tgataggttt aaaggggaat atcttgtccc
     9121 ttgtgatgaa tggttaaagt ggctatcaaa ttttagtggg tctgcgggtt tttccattat
     9181 tctcaaagat aa
//
LOCUS       DAIAHG010000031         9081 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_31_length_9081_cov_3.547423, whole genome shotgun sequence.
ACCESSION   DAIAHG010000031 DAIAHG010000000
VERSION     DAIAHG010000031.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31_length_9081_cov_3.547423"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2582 a         2138 c         1767 g         2594 t
ORIGIN      
        1 ttcacggatc atttctcttc ttgaatttat ggagactgtt tccattttat gtcatgaggg
       61 ccatgtaaaa attgatattt tgcaaagact ttttggggga tctttcgagc gttattttca
      121 ctatcttcat cgctatattg actttaggcg cacctatggc tcaccgcgcg gcgtgacgtt
      181 taaaagtgat caatttctct atcataacta tgaaaccctt gtctctgagc tgcttagaaa
      241 ataagttaat cattcggcaa agagagctca ggcatagcac tgtttagtcg ctgaatcaca
      301 agatctgtaa tatctaaatc agttttttga aaaagaacaa tcgccgcctc tggaccaagt
      361 gttttattga aaacaattgg cacattcaga tcatccgcaa gctctgcaat aatcgctgtc
      421 aacttttctt caagctgcgc agaaagcgta ttaaactgct cgttcagtgc ttcttttttt
      481 tgctgcacaa gatgctcaag ctcagctact ttacgatcaa aatcttgccg ttgtttgagg
      541 agcgcttcat ccggcttatc cgctattttt tcacgatcac gcaattgttt atgctcctga
      601 cgtaattcat tttcagaatt gaaaaaatgc tgactggatt cgctatagcg attttcaata
      661 taattcttga gcgctttaaa aggttcactc tcaagacgca cccgcaccat atccacaaca
      721 gcagcgctgt gcatcttcct ggatgtttga agaccgtgtt gaactcctac tctctcaaca
      781 aaggattctt ttaccgtaaa aaataaataa acaaaaagaa cccccgctaa aaatgcaagg
      841 gttgcataga gataaatttt ctttttatag agcatcagag tttcgattta aatccgataa
      901 ggaaccgctg tgtatcatcc attttatctt ttctaaaggg tgttgcatag tcaatacgaa
      961 tcggtccaaa aggtgaattc cacacaagac ccacacccca tgatgcccga atatacttat
     1021 tatcacgcac gaaaggacct gtttctgtgg atttccaaat cgtacccaca tctgtgaaca
     1081 cagcaccctt cacaccaaat tcattcggca aaccaatagg gaacatcgct tcagctgttg
     1141 tcacccaaaa acgcgtacca ccaagcggat cgcctgtgcg gttatcatgg ggaccaagac
     1201 caccgtactg gaagcctcgg aaagagtctg caccaaggaa gaaactatcg acgatgcgaa
     1261 tacgttttcc tgccttttgc atcacaccat aacttccctt gagtgccatc acgacgtcct
     1321 caatggggga gtaataaagc gtccccccta ggatgtgttt aaagtatttt gcctgccccc
     1381 cgagacctgc gtaggagttt gagagtgtca aaacgtaccc acttgtggga tcaatacgac
     1441 tatcacgtcg atcgtaataa agcgtttgac caatttcaga ggttacatat cctccacctt
     1501 gtccgcgcaa gaaactagaa gcctggcttg aaatatggct cacacggtct tgcttgatag
     1561 tgtaactcag aacctgcgaa aggtgctctg tcagcctata cccaatactt ggagaaagac
     1621 cttttgtaat ttgtgtatag gagctaaaac gcgttgaacg gtacgcaaaa gcatccaaac
     1681 ttccctcaag ctggcgacca aaaagatacg gctcgacaat gccgacatta atatcttgat
     1741 ggcgttttga aattgaaagc tcggagtgaa cagtctgccc cttgcccata aaattccgct
     1801 cgagaaaacg tattgtccca agaggaccat caatggttga aaatcctgca gaaaaggaaa
     1861 gctcacctgt ggattgctct tcggttttga caagaagaat agcacgatcc ggcgtactcc
     1921 cttgctcaat aataatttcc acatttttaa agtaaccaag gtctttaagg ttgcgctcgg
     1981 aacgcttaat gcgcgacgtg ttcagtgcat cgccctcatg aatggtcaac tcacgacgga
     2041 tcacataatc acgcgtgcgg tcattccctt gaatatcaat gcgctcaaca tagactttgg
     2101 ggccttcttt aatctcaaaa acaatgtcaa ctttttgcgt ttctggatgg cgcgtcgttt
     2161 taggcacaat ttctgtaaag gcatagcctt tgtttccaag cgcatccgtg attttctcaa
     2221 tcgtgcgctc gatcatcttt gcactaaaaa catccccact attaatcaaa atcagtggtt
     2281 tgaactcatc cgcattcaca acaggaatga gcgacaccag accaaccgtt ccaaacgtat
     2341 acgctttgcc ctcatcaata ctaaaattta aatagtagct attatagtca ggtgtgatct
     2401 ctgccacagc cgctatttgc tgatagtcag gatgaccgtg atcataatag tattggcgca
     2461 aactctgttg atctgcatta aaacgcatag gatcaaaggt gtcatcattc gcaaaaaaac
     2521 ggaaccaacg gtattctttt gtgatcaacg catctttgag ctttgattgg ctgtagtgtt
     2581 tattgccgat aaaattaatt ttgcgcacat acgtcgtttc gccctcatga atctcgaaca
     2641 cgacatcgca gcgattatca tccaattgaa taatttttgg ctcaaccgtc gccgcaaagc
     2701 gccccatcgc acggtacatt tctaaaatac gctgctgtgc tgcctgaatt ttcgtccgcg
     2761 agagaacctc ccgtggtcgc aattgaattt cttctttcag ctgctcttct ttgagcttgc
     2821 tattcccctc gtaggcaatg cggttaatga taggattttc ctcgacactc acaacaaggt
     2881 tgtcaccctc tacactgacc ttaacatcat taaaaaaccc tgtggcaaaa agctccttaa
     2941 gcgcctgatt cacgtcatcg tctgttgctg tttgacctgg cttgatacga atatacgaaa
     3001 gaatggtatc gctttcgatg cgcttattcc cagaaatcgt aatacgctga atcgaaatag
     3061 tttccgcaca aaccatagag agtgtgctta gactaaggag caaagcactc gtgataaggg
     3121 ttattttttt catatagacg ttcaattcct ccaaaaagtg agatagattt attttgaggt
     3181 gagatggagt atttggccta aagactcaaa cactttgagg cggctaaggt cattccatgt
     3241 cacaaaaagc atgagactca caacgataat gaatcctgca agaaaagtat actcttgcgc
     3301 cttcacagga acagggcgcc cacgtacagc ttcgataatg taaaaaagaa tatgcccgcc
     3361 atccagcatc ggcagaggga aaagattcaa catacctaag ttaatggaaa ggagtgccaa
     3421 aaaccaaata aaacttgctg cacttgattg cgcacttttg cctgccattt cgccaatgga
     3481 aagaataccg ccgagctctc ctcctttgcg tttaccaatc actgtttcac taattgtgac
     3541 aacgacgtcc cgcgacatct cataggttgt tgtaaatgcg gatatgaccg aatcaattgc
     3601 accacgcttt tcaaattcag gcgcgcctgg aaccacacct aaaacaaagg gttttgcgaa
     3661 cgtttctgtt tttttaagag aaagcaccac aggttcatta tcacgctcca ccgtgacttt
     3721 aagatcatgc ccttcgaatg ctgcaatggc cgcacggagc tccttaaagg ttgcgacttt
     3781 tgttccatta aactctgtaa tgcgatcatt tgcctttatc ccaagctgca tcgccacact
     3841 gtcggggcgc acttcttgga cggttgaggg aaggactgga tgacctttaa ccagaaaaag
     3901 aatcgccatg gctaccacag caaagagaag attcgccaaa ggtcccccga aactaatcgc
     3961 catgcgttgc cacacggttt tgctttgatg ggtcagcgca tagtctttgg cattcatctt
     4021 agatgaagcc tcgtgatcgg gccggctcgc agcatccgca tcgccaaaca tgcgcacata
     4081 gcccccaaga ggaataacac taaatttcca ccgcgtgcca tgtttatcaa aaaaaccaaa
     4141 aagctctggg ccaaatccta tggagaaaac ctcgaccttt acaccgttac ggcgggccgg
     4201 aaaataatgg ccaagctcat gaataaaaac taaaaccgtc aaagccacaa caaacggaag
     4261 gacgtactca acaaaaaaca tcatttctca gaaaaataaa catagatttg agacatctta
     4321 gcgaattgtc atgcccttgt ccataaagtg ttaccttaga acgtttcttc tatggggata
     4381 atatggggat aatatgggga taaaaggagg cgggcgcctt ctcatgtact cgtgctcatg
     4441 tcatgttagg aaagaggata gccgtgacta ccctctatga tatagttatt tttaaagatt
     4501 tttcccctca ataagatcgt gatgctcctt aacaaccttc attatacgcg atttgagttc
     4561 ttggtctttt gtcttaaatg tagcagcaac atccataagc tcctttacaa gggccttagc
     4621 ttttttttgc gatgtaacat caggctttac tttcaattca tctctatttt cgaaaataga
     4681 ttcatatttt tttttatcta cctcagcctt gagcgcctcg tctaaagcag aactattaag
     4741 aactttaaaa tcactcataa tttcagacag atgcatcccc cactctgcat taatatgctt
     4801 tgagtgatct tgtgtatctc tcttaaggag tgcaacttgt ttttcaagtt tttgaactct
     4861 cgactcaagg ctttcatcgt tagatcctgc catcacatga gaagattgaa ctcctaccgc
     4921 accgactaaa aacaacgata ctaatcctaa ctttttcatt actattttcc ttttcataca
     4981 gattgaaaca tttattaata gtgcttttaa gcaacaaggt atattctcag ctaaaaaagt
     5041 taataattcg taaaaataaa aaacaatgat ttctttgatc cactttatct tcaagatctt
     5101 caaggtgaat caaaaaaact ttccaagaga aaaaactcta gtgaccatcc accttaatac
     5161 gatatttcaa ctcgaagggc gtctcacttg ccgcaacatt tgggatatca aaggaaacag
     5221 cctcaccatt cacgtcaaaa ggaagagagt tgcgcaccat cgtccaatga cactttggca
     5281 aggggagaac agcccggatt gaaagagggg ctttaccttt attgtgaatc acaagcctgt
     5341 acccagcttc gctgcttttc acagaattct ttttatagtc tgtttgctcg agctcgcaca
     5401 caacacttcc ctgatagaaa tcatgaccga tatcaattgg aactttcccc ccaagcggcg
     5461 tctcttgaat catagcctgg ccaataaatt cagttagacc attttcatgt ccttgaaaaa
     5521 gcgtaatttt ccccgcaggg agcgtcatat tgagcccgtt tttttcatca tttgagaatt
     5581 gaatcacacg ctgaatattg ggatgctgta tctctccttt aagatcgtca agatacgccc
     5641 caccaagatt aataatattt tcctcttcaa ggggaatatg aatggcgttg ataaagctaa
     5701 tatagatttt tttatcctca ggaagcgtga tcacacgtgg gatgagatag cgaaaatggc
     5761 tctttgagtg atccatcaca gtcagcgcat caggtgttat ttttgtcgca ctgccatcca
     5821 aatagatttg tgcttgatca atcgttgaac ccgatttatt ggtaacctcg atccacccac
     5881 ttagcgtgag cgttttttga tcactcgata aaattcctgt gtaataaaca tcccatgtga
     5941 tccctgaaaa aacatagtaa atgttgagaa ttttaacctg atcataacta cttaaaacct
     6001 caagatttag cttttgcata tcatcactga taaattgtcc caaaagctgc atatgagcat
     6061 cctctgcatc taatgttaca ataagacttg tgggatcaag cgtctttgga attttatcat
     6121 aaatcagatt ttgataacct gttttgagat ccgctttgat ttttttaaga gcagcggcag
     6181 taccgttggg atagagagtg atattaatga gggccgcaga agatgaagga ggagcgggtg
     6241 ctttttgcgc atcggcataa agggtgctga tccctaaaac agcgatcatc gccaatctat
     6301 tgatacattt tctaagcagc cttagttgta gcattcgata taacatcatt atgagcatca
     6361 tcatggcttg cattttcctc aaaagacaga tctttattgt tactggattt ttccaagagt
     6421 tcaagggctt gtatctggcg gggcatatgc gcaagggaag gaacatcact aatgtcctcc
     6481 gcatgcgtgt caaaatcctt gaattttctt gcagaaacga gaacacggcg ctcgagtgtc
     6541 cccaccgttt gattatagct atcgaccaca tgaccaatat ttttaccaag acgcaccata
     6601 tgatttgcca gatcagagac acgtttactc aattcttggc caagcttact aatcgccttg
     6661 gcattttcag caagggattc atggcgccaa ccataggcaa cggcacggag caacgcaatg
     6721 agggtggttg gcgttgcaag aataactttg tccttaaaac ccaattctaa aagctctgga
     6781 tcttgctcga gggccgcact aaaaaacatc tcacccggga gaaaaagcac aacgaaatcc
     6841 ggactttgct caaactgttc aaaataggaa cgctggctca atgctttaat atgagcacgt
     6901 acatgtttcg catgctcttg gagtttttga agacgaagat tttcatccgt catatcaaga
     6961 gcttctaaat acgccataag aggagctttt gcatccacaa taattttatt atttccaggc
     7021 agatgaataa tcatatccgg gcgcatccgt gcaccactat cactatctat cgcagaggat
     7081 tgctcgataa agtcacagtg agatgtcatc cctgccacct cgacgacgcg gcgcagttgc
     7141 atttctcccc actttccacg cacttgaggt gtgcgcagcg ctttcacgag attcgctgtc
     7201 tcaaaacgca gctctttttg tcctgaaatc agatgatgca cttgtgtttt aagcgtttca
     7261 taagccccta cgcgctcttt ttcaagggcc tgaattttaa gatcaacttg ccccaaagct
     7321 gtttgaatcg gctgcacaag tgtttgaatc gcctgctctt ttttttccaa agaattcgcc
     7381 atatcgagac ttgatttttc catagccgcc cgcaatgttg aatttgcaag gtctaaaaaa
     7441 gatttattgc tctgagcaaa aatttctgat gaaagggctt taaactgtgt ttgaagttta
     7501 tcctccgcat tttgcaaaag gcggagcttt tctgctgctt gcgtttgctc ctgttgaagc
     7561 gtcgccttga gataactatt atcctttaaa agctcatcga ctttctcacg ctgttgcgca
     7621 aagttttctt gaatatcttt gagaaaacta agttccgccg ctttacttgc attttccccc
     7681 gtaagttctg tcacggtcag ttgcaaattt tctatttttt gatcacgctg ttgtaactga
     7741 tgcgtcagag catctttatg ctgctccgcc tcacgcaaac tatgattaag atgatgaatt
     7801 tcatgggcaa agcgcgtttt caatgctcgt tttctgtgct gtgatgaaag ataaatcccc
     7861 atagctgcag caaaaaaaca aacacacaaa acgcctaaca aaatctccag gaatgtcata
     7921 aactaacgtt catcatcaga atgtttttca tcagaaggta actcctcgaa agtatcagcc
     7981 tcagtttcgt ctttctcaat acagctaact gcaacaactt tttcattggc attgacacga
     8041 aaaacgatca caccttgggc attacggccc gtcatacgaa tatcgttcac agggcaacga
     8101 ataagctgcc cctgatccgt cacaagcata atgtcgtcat tgtttgtcac aggaaaagac
     8161 gcaatcacag gaccattttt agaggtaatg gagatattgc gaatgccact tcccccgcga
     8221 tgaatcgtgc gatattcata aaaggatgtg cgtttaccaa aacctttttc tgtgacagtt
     8281 aaaataaatt cctcacggtc cgcaagctct tgaagacgct cttgtgtcaa ctgcaccgtt
     8341 tcaacactta aatcctcggc tccatcctca tcgtcttttt cagtcgattc atcaacactg
     8401 tcatcgcttt gacgtaattt cgcagcatgt ttcaaatagc tatagcgttc tgcgattgta
     8461 atatcttttt gatggcccac aacggtcatg ctcatgacgc tatctttggg cgcaaggcga
     8521 ataccgcgca caccatttga atcacggcct gcaaatacac gcacatccgt cacttcaaaa
     8581 cgaatcactt ttccttgatg cgtaaagagc ataatctcct catcatcatg gcaaacgcgc
     8641 acagcaatca gcgactcatc ctcgtccaat ttgatcgcaa tttttccaga agcattaata
     8701 tttgtaaaat ccgacacacg gttgcggcgc acatgtcctt ttgaggtcgc gaacatcata
     8761 tacgttgtgt tcgcatcctc aacatctggt agcacgagaa ccgttgaaat tgtctcgttt
     8821 tgttgaagcg gcaacaggtt cacaataggt cgcccttttg actgtggatt gccaagagga
     8881 agcttgtaaa ctttgagctg atagactttc cccgtagtgg agaaaaaaag cacaaggctg
     8941 tgggtattcg ccacaaaaat atcgcgaaca ctatcctcat ccttggtcgt catccctgaa
     9001 cggcccttgc ccccacgttt ttgcgcacga tacgtatcaa gtggcacacg tttgatatag
     9061 ccctctaagc taacggtgac g
//
LOCUS       DAIAHG010000032         8956 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_32_length_8956_cov_3.811578, whole genome shotgun sequence.
ACCESSION   DAIAHG010000032 DAIAHG010000000
VERSION     DAIAHG010000032.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8956)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8956
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32_length_8956_cov_3.811578"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2536 a         1666 c         2056 g         2698 t
ORIGIN      
        1 caaggcgtta cactcgaggc gaacccgggt gaaattgtag cgatcatggg cgagaatggt
       61 tcaggaaaat ctacactttt aaagctgctc ctcgggtttt atcaaccggt tcatgggggc
      121 atttatctgg atcaaattga tattaaacaa attgccgtga accacttaag gcaatccatt
      181 agttttgttc ctcaaaacac acagtttttt catggaacga ttgagcaaaa tcttttgctg
      241 tccgcaccct atgcaacgcg ccagcaaatg attgaggctt gcaaaaaagc tatggtgtgg
      301 gatgatattt gtagattaga acatggtttg gatacaaaac tgacggatcg ctccacaact
      361 tttttgaatg cagggttcca gcaaaaaata tcattagcac gctcttattt gcgtgatagt
      421 ccagttttgc tttttgatga gcctgggagc aatcttgatt ttcaaggtga cctttggttt
      481 aaggaaacac tgctttcatg gcggggggaa aagacgatga tgattgtaac acacagaccc
      541 agtattatta gtctttctga tcgtcttctt gttctctcag aggggtatat gcgttatttc
      601 ggaccgactc aaaaggtgct tgagctgatg cgagaggaaa gtgcagcatg acttatttta
      661 ttgatcaccc ctcgatatct tctgatgaga aaaaaagtac tcccttcgcg aatgcaacgg
      721 aattattaga gagtgatata acagaggaag aaaaaattgc tcaagaaaat tctgataatt
      781 ctgatcataa aggacacgca gataaagaaa aaaatacgaa aagccatcgc ctgagtattc
      841 tcaaagggac agataaattt atgctgatga agcgcggtct tcctgagctt gggcggccca
      901 ttgctttatc tgaagaggag cttccgagag ggagccatct gagcattctt gtcatttgcg
      961 cgatgctttt gacatttgtt ctttgggcgt ctgtcacacc tattaaagaa attgccgtgg
     1021 ctcctgggca agttgttcct tcgggatttg tcaaaacagt tcaacatctt gaggggggga
     1081 tcataaaaca tatttttgta caagatgggg attatgtaaa ggaaggggat acgctgatgg
     1141 cgctggatcc tgcagcagct ttttctgagt tagagcagct caaagcacga gaagcttctt
     1201 tgcgcatcaa ggccgaacgt cttaaagcat ttgggatgaa tcaagagccg gattttaaat
     1261 cctttgatcc gatctatcaa aaacttatcg atgatcagca agcgattttt gacatgcaaa
     1321 gtaaaaacag agcggatcaa agggatatta ttctcaagca aattgaccag cgcaaagccg
     1381 cactccatgt ccaagaaggt cgtgtgcagg atattcagcg tcgtatcgat gtgctgcaaa
     1441 aacagcgtga tgtgattaaa ggtctttatg agaaacgtct taaaacaggg tctgagtatc
     1501 gcacagccga ggattctctt gctgaaatta ctgtagatct gaatcaggct aagaacacag
     1561 cccttgaaac agaacagggc attcaagaac ttgaaagtcg tttgatcgaa ttggatacgc
     1621 ggctgcgcaa tgaatcgctt atcgatatgg gcaatgtcgc agcggaaatt gcacaaatca
     1681 cagaagctaa aataaaactt gaggaccgtg tcaggcgcct tgaaatcaaa gcgccgtccg
     1741 agggcattgt aaagggcatt aaaaatacaa cccttcaggg ggttattcaa ccaggggcag
     1801 aaattatgca aattgtaccc cgtgacagtc ttgaggtaga ggcaaaagtg cagcccaaag
     1861 atattggtaa attacatcct gatcaacatg ttttggtcaa ggtatcagcc tatgactata
     1921 cgcgctatgg gggaattgat ggcaaagtaa aatctatttc agcaacgaca ttttctgatg
     1981 ataaaggcaa tccttattac agagtgttaa ttcacttaag caaagcgtat gtcggcaaag
     2041 acccaacgca tcatcgtctt acacccggta tgacggttca gacagacatt gaaacagacg
     2101 aaaaaacact ttttcaatat cttatgaagc ctatttatgt cgcgcttcat gaatctttta
     2161 gagagaagta aatttgcttc tctctatgtt gaaaaattct ccgggcttgc tctcgtattt
     2221 tttctcagtt acaaaagaga ataacactat ttttatttta gtatcgaatg cgcattgaga
     2281 aacgacaggc aggggatcta cgccctgttt ttttaggtcc agggtttgca aaatttgcgt
     2341 ttgcttcctg ttttgtaaaa tttggtgata cacatgttct ttgcacggcg agtgtggagg
     2401 aaaaagtgcc tccttttttg cgtaatacag gatccggatg gattacagca gaatatggca
     2461 tgttgccagg atcgacgcag caacgggtgg agcgggaggc tgttaaggga aaacaatccg
     2521 gccgtacgca agaaattcag cggctcatcg gacggtctct tcgctctgtg atcgatctta
     2581 aaaagctggg cgagcgtgtg attaaaattg actgtgatgt gttgcaagca gatgggggaa
     2641 cccgtacggc aagtattacg ggggcttatg tcgcgatgta tcaggctgtt caaaagctgc
     2701 agacgcaaaa attgcttaag gactttccca ttacggatca aattgcggct atttcctgtg
     2761 ggatcgtgaa tgacgaggca cttttggatt tgaactacct tgaggattca aatgcggagg
     2821 ttgatgcgaa ttttattctc acaggatcag gaagtattgt agaggtgcag gcctgtgcgg
     2881 aaaaaaaagc tttttcgagt aagcaatttc aacagatgtt tgcccttgcg gaaaaaggta
     2941 tttctgaact gatcatgctg caaaaacagg cgttaagaat ttaatcgacg ggcttagttc
     3001 tcccaatttt tatcattttt aagataatcg aggacgagct ttttgacaat tttttgctgc
     3061 tcttcattga ggtcattgaa cgcatcaaaa tagccatcca ggagcgcttg ataataatct
     3121 ttttgttttc tatacgggtg gctgatcgca gaaagtgtac tgccaaggcg tgtgaaatca
     3181 tctttttgag cttttttata ataaacggca gcgctttttc caagacttga gatgtcaatc
     3241 cagctaaatt tttttgtttt agggtcatag aaaacattgc caggatgggc gtctaaaaaa
     3301 gtcatggtat aaagtgtttc gggcttctcc cagttgccgc ggaggaaatg tcgctcaaaa
     3361 agaccataag ctcgccctag agcaaagaaa ccttctttca gtttttccgt tgatttctct
     3421 gtcgcataat aggtgttcca gggcattcct tttgcagcat gaaggaaaat aaaagcgaca
     3481 ttcttataac caatttttac tttgttacgt gtgaggaaaa tcttttcatg aaatttaatc
     3541 tgagggattc ccttaatttt ccaaagacca ggaagcagcg cacggctgaa cataaggccc
     3601 ttaatctctc cctttatctc tcctgatttt ggtactttaa taacgtaaag cagcttaaaa
     3661 tcttctgatt ttttatattt aaccttgagg aaaaaaaggg cttctgttcc aaagccatta
     3721 aaagattcaa ctttctctag tttaagatag tttttattcg gactaccgtt ttcgtcaaaa
     3781 caataatcgg gcacaatctg aaaccttttg atgaggtaga gaattttttc atcgaggcca
     3841 tgaattttat aaaaatcctc gtatttcttt tgttcgaggc gataaatatc aagattttct
     3901 ttctgataca ggtctttttg attgaaacct gtgacctcaa aaaatttttc ttcatcgaca
     3961 ccttctagaa aatcattttt aatggctttg agttcgttta tttgtttatc aagagaatca
     4021 ttatggttta aaattttttc tttctctaag gcctgtattt tctcatcaag agcatcaagt
     4081 gcgtcccaat cttgatcctc tcttggtgta ttagattgca gagcatgctt tcttagcttc
     4141 aggcggttgt tcaggtattt tattttttga tttagctgat ttagcgcttc tgtcgtcttt
     4201 tcattgccga tagtttctat cttttctgat gcatggagat atccaaacat caatataatt
     4261 attatgagaa tgatcatccc aaaaattcct tagcgtataa actgtatcac tggctatttc
     4321 ctctaaagta tgcaagaaaa aggtaaagaa agtataaatt ttgttgagcg gatgtttttg
     4381 aatacgatta cgcccttcct tcttgaaaaa acagctgtgc gcggtcgttt tgtgcgtatg
     4441 gataccgtcg tgggtgaaat tcttaaacgg catcagtatc cagaaacgat tcatcactat
     4501 ttagcagaac ttattgttct tggggtgact ctttcaaatg cttttaaatt tgacggtgtt
     4561 tttactctac aagttcaagg aagcgagaat tcgcctgttt cttttatggt tgtggatgtg
     4621 gactcaaaag gacatgtccg tgcgtgtgcg cattataaag aggagcattc gccgtttctg
     4681 tctgcgaaaa aacgcacgtt gatggaactt tttggggcgg gttaccttgc tttttcaatt
     4741 gatcaagggg atgatcttga gcgttatcag gggatcgtcg agcttgaggg ggttacactc
     4801 aaagagtgtc ttcaacatta ttttaagcaa tcagagcaat tagaaacggc tttttatctc
     4861 aacctcacaa aggattacca gggtgtgtgt atcatgcttc aaaagcttcc ctttgatgca
     4921 gagacggata acgaggcttt tgatgattgg tttacggcaa tgtctttgtt atcaactgta
     4981 aagggagagg aagcctggca tttagggccc gaagagcttc ttcatcgtct tttttggcag
     5041 gagggactga aagtttatga tgctaagact gttcaatttc aatgccgttg cagccgtgaa
     5101 aaattagaag tgattatggc gcaactttct ttagaagata aacaagccgt gacaaaggac
     5161 ggcgtaatca cagtttcttg tgactactgc ggtgaagttt atctttttga agcgggatac
     5221 tagtattccg cgtcttttgc gtttttattt tttttcagcg acaaatgctt tcataagatc
     5281 gataaattct gccatttcaa aatcacgacc gatggaaacg cgcatgtggt gatggagatt
     5341 ataaccgcta acagggcgga tcatcacgcc gcgtgtgcca agataatgat aaatatcctt
     5401 tgcacgctcg ggcgtttcgc aatcaaaaag aaggaaattc ccctgacttg ggttcacacg
     5461 aaaaccaagc tcttcgagtt tttctttggt ccatgaacgg tgtgcaagat tgtgctcaaa
     5521 ggcttttttt atccacccct gatcatgcac agcaatggtg gcgctgtgtt gtccgatact
     5581 atttacatta aaaggctgtc tgatccgatg aagcgcatcc agtacctcag gtgcaccatg
     5641 cgcccaaccg atacgcatgc ctgcaaggcc ataagctttt gaaaatgtgc gcaaagttat
     5701 gacgtttttt gcatccttaa aaagggttaa gccactttca taatctgtat gctcaacata
     5761 ttcagcatag gcttcgtcca ggactaaaag cacgtggggc gggaggtttt gatggagtgt
     5821 taaaagatca ttgcgtgtca gcatgcaccc aagggggttg cctgggttgt caaggcacac
     5881 aattttagtt ttcgctgtca catgttccaa aagacgatca acttctgagt ataaatgggg
     5941 ctgtggaaag aaaacaggtg aagcaccgtt cgtcttagcg gcaatcgaat acactaaaaa
     6001 gccatattgc ggcatgagaa tttcatcccc ttcaccggca taggctcggc aaattaggtg
     6061 aataatatcc tctgagccat taccacagat cagatgatcc attgatcgtt gatggacact
     6121 ggcaatcgct tcacgcaggc gagaagcacc tccattagga taactggaaa gattaagatt
     6181 aaaatttcta aaaaactcca gcacaagagg cgacggccct aaagggcttt cattcgaggc
     6241 aagaacaatg cgacggacca ttcctttggg ggtaacatca ccaccgacat aaggatcaat
     6301 tgtcataacg ctgctgcggg ctttgggcat gagagaagat tttgtcatat ttttttatat
     6361 cattattatc aatttttctg catcttaacc ttcctttgtt ttttgtgaaa gagtgaaata
     6421 gagaaaaaac agtatttcta catttctatg atgcgatggc atggggccaa tcattgttgt
     6481 ctgtaatggt tatccataca acttcttgga gatgtgtata aagaagcagc cctttgatcg
     6541 tgtggttggg gtacatcgat tttagatttt ctgcgtagcg cttgatttgc tgagtatgct
     6601 caatgtgggt gtaaggagtt ttcatccctg tttttatatc gatgataatc gcttccgttg
     6661 cgttgatcgc aaggcaatca aggcgatgaa gttttccttc ttcatccttg atcgaaagct
     6721 cgtgaaagag ctgcattgtg cgcgtaagag gctttatgtg attgttatag agatggatga
     6781 tcgactcaac ttctttttct tggatgtctt tgaacagagg gtgtgttttg agatagctct
     6841 ttgaatgaag gctaagcagt tcaaataatt tgtgcagtaa aatacctttt tctgaggagg
     6901 cagacgctgt tgattttaat gattttgacg gcgcggagag agcgaggtgc tgcgcttttt
     6961 ctgttcgcac agatgggttc actgaggaag ttttatgttc atccgaatgc ttctcaagat
     7021 aatgatgata atcagtgctc tcatctttct ccataagcgg tgaaagaaga gaataccaac
     7081 tttctttttc tcctttgtca agacctgtga tgaaaagata atcttgggcg cgagtgagcg
     7141 ccacataaag aaggcgatga ttttcctgtt cttgagtctt tttttcgagg tttttaaggg
     7201 gctctagtaa agtattttgt tggcgcagtg cgtgaaaatc gtttgtgaaa agaatattat
     7261 taaacgatcc tccctcaaaa tctcccgtat ctggcaaaat aacaatcggg gattgaagtc
     7321 ctttcgatcc atgaatcgtc attatacgca caccggatgt atgagacgcg ttcgcagttt
     7381 tgagaagagg tgcttctctt tgaataaaat gagcaagctc ctcaaatgtt aaaatacttt
     7441 tttgcataga ggtttgggta agcgtcagca aatgttcgat gaagggctca ataatcgcgc
     7501 cgaaatgctc aaaaaagcgc gcataggtgg tgtgaagata ggtgtgtatg aatgtataaa
     7561 gatcatgatg ttttgaaaat tctataaaat aatggagaaa atcaacgctc tttttgtaaa
     7621 gagggtgatc agaataatat tgaagggctt cccacagggg aagtgtttta tctttgcgca
     7681 attcctggag agctttttca ttaaacccaa aaagagggct tttgagtaga gccgccagat
     7741 ttaaattatc gtaggggcag ctgagaaaaa ataaaaaact tagaaagtcc ttaacatata
     7801 aatgctctaa aaaagcactt ttatcctggc aatgatgatc tatccctgca tgggtaaacg
     7861 cctttgaaag ggcattcatg aggctaccac gtttacgcaa aaggatgaaa atatcacgcg
     7921 gccggatctt ttcaccggtg gacggcaaaa gcatatcgct tttcaaaaga ctttgaaccc
     7981 gtgcggcaag cgtctttgca agaatatctg cctcagaatt ttttgttaga ggcggctttg
     8041 tgtagagtgt ccatccagca tctgtaggcg aatctttttt cacagcaaca aggggccacg
     8101 ccacggcttt tccaatcact tcgcggtgag ggtgatgatt gattttatgg gggcttaaga
     8161 gacttttaaa aagggcgtct tgattgcaaa cttgatctac aagggttaaa agcgcagggg
     8221 cacagcggta gcacgtttca aggctaagag tatgaagatc aaatcctgat tgattcagga
     8281 gggctttaaa ataatttttg tttttatgat actcaagagg gtcagcccct tgaaaactat
     8341 agatagattg ctttgtgtcg ccaacaacaa aaagggtgcg aggaagtaaa tggcccgagg
     8401 tgctttgcgc aggtgtttca aaaaaatggg gagtgagctc gcgtataatg tcccattggt
     8461 caggacttgt atcttgggct tcatcgataa gaatatgatc aaatccaccg tctaatttat
     8521 ggtgcaccca cggcatatga tctttgagaa gctttttgac ttttataaga agatcatcaa
     8581 agtcgagaac attaagctcc tccttcatac gtgtatagcg tgcgagaaca cttgtataga
     8641 cagcgtcaaa agcattattt gtctctatgg tgaaaaggct ttgctggtat tttttaagag
     8701 cgagaaattt ttcggcataa tgatcaaaaa ggtcatcaaa atcgggaaaa tgttttaaga
     8761 gttcctttgt agatgctttt ttgcggggta gatcctcggc cgtgagaaaa aaagatatga
     8821 gctctgtgtt ccctttttct tctgtcttta agagcttctc tgcctgtgtt ttaagtgatt
     8881 ttggcccata agattctact tctgaaataa ggttaaaaat attttcatga tcatcctctg
     8941 tgaagggggg gggaga
//
LOCUS       DAIAHG010000033         8895 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_33_length_8895_cov_3.149807, whole genome shotgun sequence.
ACCESSION   DAIAHG010000033 DAIAHG010000000
VERSION     DAIAHG010000033.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33_length_8895_cov_3.149807"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2562 a         1704 c         2114 g         2515 t
ORIGIN      
        1 aacaggtcgt ctttcccgca ccgttagggc ctaaaaggcc aacggcctcg ccctgttgta
       61 cgtcgatatc aacgccaagg aggataggtc tttttccgta gcttttgctg aggttgcggg
      121 caacaaggcc tgtgagggag gattgattag tcatcgtatg cgttaagtgt ccttgtctct
      181 ttgcgttttt ttagatcttt tcactttatc tattgcattc taaacatata agaccccaaa
      241 ggccagacgg aagtgcgatg atcgcttttt cagagggatg ctttatagag ggtaatcact
      301 aaaattttaa aaaaggcctc ttgagttgca gcctcaaaca ttttaggatg gagactagga
      361 agattttgat gcccatgagt ccttaatgtc agcatttttt aatgagttgg cgtctcttga
      421 agggcgttat gccaaggctt tttatagtac tctatcatca acaccggaaa aattacaaaa
      481 agcactgaag gaaatcgagg attttaatgc actgctgaag gatcataagg acttttcttc
      541 tgtggtttta gagccccttg tccatataga tgtgcgtttg agagtcttag aacgtgtgct
      601 aaaagcgttt gatttttcaa aagaatttag acattttgtg ggccttgtgt tggagcatgg
      661 gcgcatgcgt tatatgaaaa cgatttttgc tgcatttatt cgcctttatc aaaaaacaaa
      721 ccatattacc cctgtgacgc tgtcatctgc acatgctttg tccaaagatg agcttgcaat
      781 gtttacaaaa cttttcaaag ataagttcgg accctataca gaggtgagtg cgaaaacgga
      841 tcccgcactc cttgcggggt atcggattga aacacctgaa gaggtttttg acggatcttt
      901 tcaaaaacaa ttacgtctta ttcaacaatc ttttcacgaa aggtagctat gtctcttcgg
      961 cctgctgaaa tttcatccat tctcaaagaa gaattggggc attttgaatc ccgccttgac
     1021 gtgtatgagg tggggcacgt tctcaatctt ggtgatggca tcgcgcgtgt tcatggctta
     1081 tcgaatgtca aagcgggaga gcttgtggct tttgcatcgg gtgttcaggg catggcgctc
     1141 aaccttgagt cggataatgt gggcgttgtg atttttggca gcgacagtgc gataaaagaa
     1201 ggggacgagg tgcgtcgcat caatcgcatc gtggatgtgc ctgtgggctt tgagctttta
     1261 gggcgtgtgg tcaatgcaat tggcgagccg attgatggca aaggtgctgt cataacgaaa
     1321 aatcgccaag aagtggatgt gaaggcaccg ggaattattc agcgtcagtc cgtgtcagaa
     1381 ccaatgcagt cggggattaa agccattgat gctcttgtcc ctgtgggacg cggtcagcgc
     1441 gagcttatta ttggagatcg tcagacggga aaaacaaccc ttgcgattga tacgattatt
     1501 aatcagaaaa aagcgaataa agaagcgggg gatgatcacg ctaaaaagct tttttgtatt
     1561 tatgtggcta ttgggcaaaa gcagtcctct gtggcgcaaa ttgtgcgcac gcttgaagaa
     1621 caaggggctc ttgattatac gattgttgtg gcggctacgg cggcggatcc agcgcctatg
     1681 caataccttg cgccctatgc agcctgtgcg atgggagaat attttcgcga tcatgggatg
     1741 catgctttga ttatttatga tgatctttca aagcatgcgg tggcctatcg tcagatggcg
     1801 cttttgctac gtcgtccacc ggggcgcgag gcttatccgg gcgatgtttt ttatctgcat
     1861 tcacggttgc tcgagcgcgc ggcaaagcta agtgatgctt cggggggggg atcgcttaca
     1921 gctcttccca ttgttgaaac gcaggcaggc gatgtttcgg cgtatattcc aacgaacgtt
     1981 atttctatta cagacggtca gatttttttg gaaacagatc ttttttatca gggagttcgt
     2041 cctgcgatta acgtgggtct ttccgtgagc cgtgtgggtt cggcggcgca aaccaaggcg
     2101 atgaaagcgg tggcgggaaa aataaaactt gatcttgcac agtaccgcga aatgtctgct
     2161 ttttcacaat tttcgagtga tcttgatgcg tcaacgcaaa aacttcttga gcgtggtgca
     2221 cgccttacag agcttctcaa gcaaccgcaa aatgcccctc tcagtgtggc agagcaggtt
     2281 gtatctcttt ttgcaggtgt gcgcggctac cttgatgcaa ttgatatcaa agatatttcc
     2341 cgttttatgg tggagctttt agagctcatg catatgcgtt atcaggatgt gatgaaggcg
     2401 attgacgctt cggggaagct ctctgaaaaa catgaacaac ttttaaaaga tgtgatcaat
     2461 gagtacgctg aaagctttac gtagccgcat caaaacggtt aggtctaccc aaaaaattac
     2521 aacagcgatg aaacttgtgt cagcggcaaa actccgtaaa gcacaagatc agctgcaaaa
     2581 ttttgtgccc tacgaccagg gatttaggga tgcgtgggag cttttgacaa aaggatccta
     2641 ttttgaggat ctgcctcatt ttatttgccc gcaagagggg gctggcaaac ttttaattgt
     2701 gttcatggca gatcgtgggt tgtgtggttc gtacaatgcg ggaatggcac gctttattaa
     2761 aaaaaagatc gaggatgata aaaaaaacta tggcgctgta agtcttttgc ctttgggcca
     2821 caaggccgtg gattttttgc gcaaagatta tggtcaaaag tttgcagggc ttatttttcc
     2881 ctcaagagag caaaggccca cccctcttga ctatattcag acaatcggtg actttcttta
     2941 tgatcacctt ataaatcatg tttatgcctc tgtggagatc tgttacacac actttgaatc
     3001 tgttctgaag caaacaattt gtgtggaaga gcttttaatc aaaaaggaaa tgaatattcc
     3061 ttttgggttc gcacctttcc tcgagccaag tcgtaaaagt ttagtgagag accttatgcc
     3121 tctttatttg acgaattctt tgcggcgtgc ttattttcaa tcccaaacat ccgagcaggc
     3181 ctcgcgtatg cgtgcgatgg acaccgcaac aaacaatgcg aatgatgtgc taacaaaatt
     3241 acatcgtacc tataatcgta cacgtcaggc aacgattacc cgtgagctca ttgagattat
     3301 ctcgggtgcg aatgctctta caagctagtt tgagtcatta aaacagcgat caatacttca
     3361 tttaaacgag ggaatttttt atgaaaagca aaatctttgt atcattctta ctaatgtttt
     3421 ttcttcaggg attagcattt aataaggtga ttggttcaga agaagagaca ggagggcttt
     3481 tcatctgcct tgagtctatc caagatttta aagaaaagaa aaaggtgaat tttaaaataa
     3541 atttggagca aacgaggaaa gagaatgagc ttacagagga agatgagact tatatgaaag
     3601 aagtagtaaa ggagattcag tatatgaatg atatacgtca cgatatctca aaaccttttt
     3661 caggaaagat tctttttaaa attgagctaa atcgtggctc agtacggaca gctttggcca
     3721 ttttaacgat actgaaagaa aaaaataatt aaacgcccgc tcttattatg gggggctctc
     3781 ctctcaaaag aggcatcatt ttttgtacgc tttagaatag gaaaatttta tcttttcttg
     3841 taagcttgag agtaagtttt caaagagagt attttttatg gcaacggcta ctgcaaaaaa
     3901 atccaaggga gtcacatcaa caccgctttt ttcgaacgcg cagagtgagg gcaatatcac
     3961 acaagtgatc ggtgcggtgg tggatgttta ttttccgcag aatattcccg ccattttaga
     4021 ggcgcttact gtaaaaagga gtgacagcga ggatgacctt gtgctggagg tggcacagca
     4081 tatgggggag aacatggtgc gaaccattgc aatggattcc actaatggct tgacgcgtgg
     4141 tcaaaaagtt gtggcgacgg gttatccgat tcgtgtgcct gtggggcctc aaacgcttgg
     4201 gcgtattatg aacgtaacgg gtcaacccat tgacgagcga ggggatttta aagacgcaaa
     4261 gctttatcct attcaccgcg atgttcctac cttcgtagag caagatacaa aaaccgaagt
     4321 gctctctaca ggcattaaag tgattgatct cttaacacct tatcttaagg gcggaaagat
     4381 tggtcttttt ggcggtgcag gtgtgggcaa gaccgttttg attatggagc tgattaataa
     4441 tattgcaaag gcccatggtg gctattctgt ttttgcaggt gtgggcgagc gcacccgtga
     4501 gggaaatgat ctttaccatg agatgatcga gtcaggcgtg attaaactca atgagaaaga
     4561 tactgagggg tccaaagcgt ctcttgtgta tggtcaaatg aatgagcctc ctggggcgcg
     4621 tttgcgtgtg gcgcttacag ggctttctgt cgcagaatat tttcgcgatg aagaaaataa
     4681 agatgtgctt ttctttattg ataatatttt tcgttttacc caagcggggt cagaggtttc
     4741 tgcgcttttg gggcgtattc cctctgctgt gggctatcag cctacgctgg ctacagaaat
     4801 gggtgctctt caagaacgta ttacaagtac aaacaaagga tccatcacca gtgtgcaggc
     4861 aatttatgtt ccagcggacg atcttacgga tccagcgcct gccacatcct ttttgcacct
     4921 tgatgcgaca actgttctaa gccgtcaaat cgcagagctt ggtatttatc cggctgtgga
     4981 tccactcgat tcgacgtcac gtattttatc ggcggatatt gtgggcgaag agcattatac
     5041 cgtcgctcgt aaggtgcaag aaacgcttca aacgtataag tcacttcagg atattatcgc
     5101 gattttggga atggatgaac tttccgaaga agataaacaa gtggtggcgc gggcgcgaaa
     5161 aattcagcgc ttcttgtcac aacctttcca cgttgccgaa gtctttacgg gaataaaggg
     5221 tgttttagtt ccccttgaaa aaaccattga cggatttcga aaaattgtgg atggtgagtg
     5281 cgatcatatt cctgaagcgg cattttatat ggtgggtaca atcgatgaag ctgaggacaa
     5341 agcaaaaaat ttataacact aaaatttaaa aaaaataata aggataaaaa tcatgatatt
     5401 aaaaacttct ttcataacac tctcgtttct tgtggggctc acatcttttt cacagacact
     5461 tctcgctgca agtgacgata tcgagcaaga aaagatcaaa gactcaaagc atgcaaaggc
     5521 attacaggtg attactgacg agggaagctt tgcttttcac tctacgacta cagttatgtt
     5581 tgatccagaa aaagttcagg acaatggtct ttttaagtat gttcatggcc tattagaaaa
     5641 agaatggaag aaagaaacaa cgaatgagta ctggaataat gtcgccttac accccatatc
     5701 ttttgaaaat atcatttgga ttaagtttta tgatgacaaa gaaaattcct tctcgtttga
     5761 taacaacgca tcattcaaaa agatcaaaga agtttttcaa aagaataata ttgtaaaatt
     5821 tgaaattggt gaaagagaaa gtgcttgttt aattttataa atttgttatt ttgttagagt
     5881 tcgaaataaa aaaaaggaaa aaacgatgac aaaattactt aagactattt ttatactggt
     5941 ggtacttatc ggtgcacaaa cgctctcctt tgcgagtgaa tatgttgaag atgagggggg
     6001 catacctacg tattttattt ataattacca aaaaataagt ctgggagtaa tttcccccag
     6061 cgtatcgaaa agaacggata attttttcga ggagatctac acaaaaatcg aagaggatag
     6121 taatctaaaa aagattattg cttggaatct gagggggaaa aaccttagta ataatgattt
     6181 taccaaagaa gaagttataa aggcttttaa aaattttgtg attggaaaaa tttcttttgc
     6241 gaaggtgggt aaaaatattg atgggccatt ggtagttttt gattcaaaca cattggtaag
     6301 ggatgttatg aaatcttttg agaaaaacaa aaacatagag gaaggtatag aaatgcacat
     6361 tgagcaaaaa gaatcctggt gcaatatcct ttaaaattgc ataaaaataa atacaaaggc
     6421 tcagtgcaat atctgagcct tttcgtttta taggctatag gctcagcgcc ttgtgcccct
     6481 caacataatc gtaaccaagg gcctctgcca cagcattatg cgtcacatga ccgtagcaaa
     6541 tattaagacc attcatcaaa tgatggttgg ctaaaagtgc gcgctctgtg cctttttctg
     6601 caagttggag gataaaaggg agtgtcgcgt tattaagcgc cacagcggac gtgcgcgcca
     6661 cggctcctgg catattcgtc acgcaataat gaataatacc atccacaaca taagagggat
     6721 tatcgtgcgt tgtcgggcga ctggtttcaa agcacccgcc ttgatcaatg gcaacatcca
     6781 caaggacaga gccagggcgc atttttttaa gcatatcttt tgtgacgagt ttaggggctt
     6841 ctgcacccac aacgagaacg gcgcccacca caaggtctgc cgttgtgaca tagtgttcga
     6901 ttgtttctac attggcgtga ataatttgag cgcgcccttt aaagtgaaga tcgagctcgc
     6961 ccaaacggcg tggattttta tccataagaa caacatgagc ccccataccc gcagcaatac
     7021 gtgcggcatt agcacccaca acgccgcctc caagaatagc aacattggca ggggctacac
     7081 ccgctacacc accaagaaga ataccagaac ccccatgtcc tttttccagc atttgagcgc
     7141 ccacttggat ggacatgcgc cctgcaactt cgctcatggg ggccagaagg ggaaggccgc
     7201 cttgggcatc tgttaccgtt tcataagcaa tcgcaatggc tttggatgcg atgagtgctt
     7261 ttgtttgttc aggatcgggc gctaggtgaa ggtatgtaaa aagtgtttgg tgtggtttga
     7321 gcatggagca ttcagacagc tgtggctctt tgaccttaat aatcatattg gcttgatcaa
     7381 aaatgtcttc tgctgtgttg agaattttcc ctcccgcagc gatatagtgc tcgtttgaaa
     7441 atccaatggc atcgccggca tcgtgctgga taaaaacagt atgcccaaga gcggtaagct
     7501 ctcggacaga agagggggtg agacccaccc ggtattcgtg gtttttaatt tcctttggaa
     7561 cgccaatagt cgtcatacat ttttctcaaa aaatagataa atcactatct atttttagcg
     7621 gtttttacga agaagatcat cctgttttta ttttaatgaa gagattaaat ctggctaata
     7681 gcctagtaga tgttgatttg agaagaaatg atagagagaa aattatattt taaaaaaaga
     7741 atggtgcggt cgagaagaat cgaacttcca cgggatttct cccacagcga cctcaacgct
     7801 gcgcgtctac cagttccgcc acgaccgcaa gaggtatctc ttaagtacca aatgagttct
     7861 ggaaaatcaa gataaaaggt tttggagatc cattttttcg caaaattcct tccgtcataa
     7921 gggcataaaa aaaagaaaaa gcacgaactc aaaaaggatt taagaaaatt attctggtca
     7981 ttaaccaaga tttaaaagtt tagtgtttta atccataggg tagatgcatc gcaactttag
     8041 tggagaatgt atatgaaatt acttgcttta ttgagtactt tattggcctt tgctatatat
     8101 agtccgcctc aagctatgga aactcttggg gatgatctca aggaatttga cgcgtcctta
     8161 cgcgatgaat ttttaaaaat tgcacaacca aaagcgtttc aagataaaac tgactataaa
     8221 actcatgagc cagcctatct aaaagaagaa cgggacctta ttgcaggaaa aaaattaacc
     8281 cttgcaggaa tccttccctt tagaggtggc gatgtcgttg gagatacgat ctgtgcgctt
     8341 acaaacagtc agtggtctca tacaggtgcg aaggttgttg accaagaagg tttggaatat
     8401 tgctttgaat gcacaggggc tgcggatgaa atcttaaaag aaaatatgtg gccacaagtg
     8461 caaatccata aacttgacga tgttgttgcc aattataatg gtaatgtcgg ttatcggccg
     8521 attcatacag ctgcggaaaa taatgctgat gtaacgacct acgtgcattc ttggctgggc
     8581 atcccctacg aagagaggcc tcttgatctt ataaggtcaa ttggtgattg gaataaggaa
     8641 gagcatgata aatccatgtt ctgtagccag atggtggcac tactccttaa aaaagttgga
     8701 tatcttccga atggacccga cgcaaaactt gctgataact atattccccg ggactttggc
     8761 atgcaagaaa gcttagcgtt gagtggcggt gcacagctgg gtgatcttat ttatcttaaa
     8821 ggaagcccct caaaggtgaa aaaacacaaa tgcgtcttgt tttaagccgc agtttcaatg
     8881 gaaggaatca cctgt
//
LOCUS       DAIAHG010000034         8561 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_34_length_8561_cov_2.727369, whole genome shotgun sequence.
ACCESSION   DAIAHG010000034 DAIAHG010000000
VERSION     DAIAHG010000034.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8561)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34_length_8561_cov_2.727369"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2542 a         2302 c         1643 g         2074 t
ORIGIN      
        1 aaacgctaag aaataattgg tatagagaat atgccccaaa gccgccgtat ttgttgtttt
       61 ttgagagatc ccatgactaa tgacatccac agctttagta ttgctttgcc acacccacac
      121 catggccaca agttctgtca ggaaaaaaag cccacatccc gccgcaagga gaccaaaatg
      181 ctgcacccgc tccttaattt tatcaaacgc cacatcaaac atcatcacca cgaatagaaa
      241 taaaatcgcc acagcgccca cataaacaat tgctagaata agcgctaaaa actctgctcc
      301 tgccagcaaa aaaagaatag aggtgtcaag aaatgcagca ataagaaaaa gaacggaata
      361 aataggattg cgcgccaaaa taacacaccc actggccatc acaagcttaa aagcaaacat
      421 ataaaaaatt aaaagctccg gcgtcatggt ctacctatac tcctcatccg ccttaatatt
      481 ctctgcaatc gctgtctccc atatttttcc attatgaatg agtttgtcct tggaatacaa
      541 aagttcttca tgcgtaaatg ttgcatattc aaaattcggc ccctccacaa tggcatccac
      601 aggacatgct tcttggcaaa gtccacaata aatacacttt gtcatatcaa tatcatagcg
      661 caccgtgcga cgacttccct ctgtaatccc ctcacctcca cggggttcag cctcaatgac
      721 aatggcttga gcagggcaaa tcgcctcgca taatttacac gcaatacagc gctcctcgcc
      781 atttgcatag tgacgcaacg catgttgccc cctaaagcga ggactcaaag gcgctttttc
      841 attcggatag agtaatgtga ctttgcgttt aaaaagatac cgcagtgttg tccaaaatcc
      901 tttgagaata tccaggagga gaaaatcttt tagcctcatt cgacaatccc cctcaaatca
      961 aagataaaga cgaccgctgc tgtaagcacg agccacacaa gagaaaaagg caaaaagact
     1021 ttccacccaa ggcgcatcag ctgatcaaaa cgaaaccgtg gcaacgtggc gcgcgcccat
     1081 agaaaaataa aaagaagccc acaaatttta aaaataaacc aaaacgtctc agggataaag
     1141 gcaagcagtg tactattaaa aggaggcagc catcccccca aaaataaaat tgtgcaaagt
     1201 gcactcatca aaatcatatt ggcatactcg cccaagaaaa aaagtgcaaa ggacagcgaa
     1261 gaatactcca catgaaaacc cgcaacgagc tctgactcag cttctggaag gtcaaaaggt
     1321 gtgcggtttg tctctgccaa tgctgagata aagaaaatga caaacattgg gaaaagagga
     1381 atgcaatacc acatatcctt ttgcgcaagg acgatatctt ttaagttgag cgaccctgag
     1441 agcaggatga cagtcacaag cacaaacccg atcgacacct cataagacac catttgcgcc
     1501 gccgaccgaa gggctccaag gagtgcgtat tttgaattac tggcccatcc cgcaataata
     1561 aggccataca cacccagcga cgaaatggca aaaagataca aaagccccac attaatatct
     1621 gcaaccacaa acgtactgtt aaagggaatc acagcccacg ccgcaaggct gagtccaaac
     1681 gtgatgacag gtgccagcaa aaaaagaaat ttgctcgaca cagtagggat aataacctcc
     1741 ttgtgcagga gttttacagc atctgcaatg ggttgcaaaa ggccaaaagg accgcacaac
     1801 tgtggcccaa cgcgcagctg cataaacgcc atgattttac gctctgcata ggttaaatac
     1861 gcaacaccta aaagcagcgg caccacaatc aacataattt tcaatacgat ctcaatcaca
     1921 taaaaaccat aggtgttaaa aagagagagc atgctgtcag ccatgggtca ccttcttttg
     1981 atcatcattg ttgccccctt tttgtttaag ctgaagagag catgacgcca taattttaga
     2041 atttttagca atgacattgt ccatataaaa atcgctcaca ggcattgcaa aaggtcgatt
     2101 gctcaatttt gcctttgcca aacgctcatc cacgatgggc tgataatgcg ggcacatcat
     2161 cccatcggtt gcgaaaaggg gatagtcttt ttgaaggtgt tgatgtaatt ccgcacgatg
     2221 attaaaggga agggcctgac cacacatctg tgacagtacc cgtaaaatac gccaatcttc
     2281 ctttgcttct ccaggtgctt gaacagcctg acatgtttgt tgaatacgcc cctcgaggtt
     2341 gacataaagt gcattttttt ccgtatacgc acaccccggc agaatcacat ccgccgcatt
     2401 ggccccctca tccccatgat ggccctggta aatcataaaa gtattttgaa gacgactaaa
     2461 gttaagacaa tccgccccta agatatacgc taccttcaaa tgatgcgctt cagaagcttc
     2521 atacatcccc tccaaactca atcccttttc acccggtaga aaacctacat caaaggcccc
     2581 cacccttgcc gcagaaagag ataaaacatt atagccgttc cagtcatccc gaatgatttt
     2641 gtgtgtcagg caaaaagatt taacaaggtg ttcgatttta tccgcatccg catgcgtgaa
     2701 aacagcttcg cccaaaagaa tcatcggttt tttcgctgta attaattttt gtgtaaaggg
     2761 atgctgagcc tcacgcaaaa attcaagggc agtaggtttg tcgcccaaat gctcatatgt
     2821 atacgtgaga tccacagcct ctcccacaag tcccacagaa aagtcgccac tccatgagcg
     2881 ttttttaaga cgcgcattga caagagacgc ctcaagcctt gggttagcac caattataag
     2941 acagacatcc gcctgctcgg tccctgcaat cgttgtgtta aaaagatagt cagaacgact
     3001 ctgaggagta agttttgatc cattcgcacg cgactctaag ttttcagaac ctagcccctg
     3061 cataaatttt ttaagcgcat acatcgcctc aacatcacac aaatcccctg taatcgcggc
     3121 gatctcagac gcctttgccc ccttgaacgc acccacaacc gcgttcagag cctcatccca
     3181 cgatgcaggc tgaagttttc cttttttatt gcgaatatag ggcctatcca ggcgctgata
     3241 cttaagacca tcataggcaa agcgtgtttt atcagaaatc cagtcctcat taatggcctc
     3301 aaacgccctt ggcaacacgc gcattacctc aaaaggcccc cttgtatcaa tgcgaatatg
     3361 cgatcccacc gcatccatca catcaatgct tggcgttttc gtaagctccc aaggccgccc
     3421 gtgaaaggca tacggcttat ttgtcagcgc ccccacagga caaatatcga tcaaattacc
     3481 cgacagttct gaggttatcg ccgtgtccaa aaatgttgta atctccatgt gctcgcccct
     3541 gtggagcgtt ccaagctcag aggttcctgc gatttcatga gcaaaccgca cacaccgcgt
     3601 acaatgaata caccgtgtca tctgtgtttt aatgagtggc cccatgtttt tatcagaaac
     3661 agctcgcttg ttcaggtcat agcgactttt gccgcgacca tagctcatcg taatatcttg
     3721 aagatcgcac tctcccccct ggtcacaaat aggacaatcc agaggatgat taatgaggag
     3781 taactccaga acaccctgac gtgctttttg aacaacgtcc gtattggtgt ggatcaccat
     3841 cccctctgcc acgggcatag cgcaacttgc aacaggtttc ggtgattttt ccatttccac
     3901 gaggcacata cgacagttgc ccgcaatgga aagttttggg tgatagcaaa aaaaaggaac
     3961 ctcagcaccc gcttcctcgc atgcctgaag caccgtgtac ccttcgggca catcaatggg
     4021 cgaatgatta atcgttagtt tgaccataat ttacacagcc tcccgcacac gcaagcgctc
     4081 ttccatctcg tggcgaaaat gacggacaag gccctgaatc ggccatgctg ccgcatcacc
     4141 taatgcacaa attgtgtgtc cttccacctg ttttgtgacc tccaataaat catcaatatc
     4201 ttttgaggtg ccacgccctt tagaaatatt caccatcatg cgccacatcc accccgttcc
     4261 ctcccggcaa ggtgtgcact gaccacaact ctcatgcata taaaacttag acaaacgcgc
     4321 aattgccttg ataatgtctg tactttcatc catcacaatc acaccgcccg taccaaggcc
     4381 agagcccaca gcacgcaggc tatcaaaatc catcgtcaca gtttcagcaa tatcttttgt
     4441 aataagaggc accgacgatc ccccgggaat cacagcctta agattatccc atcccccacg
     4501 cacaccccct gcatgtttat ccataagttc cttgagagga atccccatag cttcctccac
     4561 attacaagga cgattcacat ggcccgagag acaataaatt ttagtccccg tattatttgg
     4621 ttttccaatg cttgcaaacc actcggcccc acgtcgcaaa attgtgggca caacggcgat
     4681 actttccaca ttattcaccg tcgtcgggca tccataaagg ccaatattgg caggaaaagg
     4741 aggcttgagg cgcggcattc ccttgcgacc ttcgaggctt tctaaaagcg cagactcctc
     4801 tccacaaata taagcccccg ccccgcggtg aagaaaaaga tcaaaatccc accccgcttt
     4861 gcacgcattt tttccaagaa atcctttgct atacgcctca tcaatcgctt tttgaagaat
     4921 cacagcctca tgataaaatt caccgcgaat atagatatac cccacatgcg caccgatagc
     4981 cgtccccgcg atcagcgcac cctcaacaag tttgtgaggc tcgtagcgca aaatatcacg
     5041 gtccttgcac gtccctggtt cactttcatc ggcattaatc acaagataac ttggctttgg
     5101 gtgactttta ggcataaagg accatttcat acccgtacta aaaccggccc cgccccttcc
     5161 tcgaaggcct gaagctttta cgtgttcaat gattgtctcg cgccccaagg ctaagatttt
     5221 ttttgtctca tcccaatctc cgcgcgccat agccgctgtc agaaaaggag agtcctctcc
     5281 gctcagattt gtaaaaatac gatctttggc attaagcatc tgtttttacc ttttttgtcg
     5341 atgttttttt gaccgatgct ttttttatag atttttcttc ttgctctgat gatttttgag
     5401 ataatgcggg caacccttcc ttggcacagg tttttaaaag cgccacaacg gatttctcat
     5461 caagattttc aaaataagta tcattaattt gcaccacggg cgcattcaca caggcaccaa
     5521 ggcactcgac ttcttcaagg ctaaaaaggc catcgcccgt gacctctttc atgcccaccc
     5581 ctaaatgctt tttgcatgca tcgcgcacag actctgcccc cctcagccaa caaggtgtcg
     5641 ttccacacac ctgtatatga taggtcccca caggttcaag gtgatacatg gaataaaaac
     5701 tcgccacctc atgcacttta agcacaggaa tatgcaacac attcgccacc gcctccagcg
     5761 cttcataaga aagccagcca ccgttttgac gttgcgcaag atcgagaaga ggcaacagcg
     5821 cactttgcga acgatcgctt gggtattttt ttaaatgctc cgcaacacgt acttgatttt
     5881 ctggactaaa agcgaacatt agcgatcaat ttccccaaaa acaatatcca tagagccgac
     5941 aatagccaca agatctgata ataaaaagcc ctttgaaacc cactccgttg actgcagaaa
     6001 gggaaaaccg ggtgcacgga ttttacaacg gtaaggtctg ttggagccat cagcaacgag
     6061 atacacccca aactcaccct tcggcgcctc aacacaggca tacacatccc ccacaggcac
     6121 gtgaaaaccc tccgtataga gtttaaaatg atggatcatc gcctccatcg aggatttcat
     6181 atcggcacgc tttggcggca ctacttttgg attattcaca gcaataggcc ctttaggcat
     6241 tttctcaagg cactggcgaa cgatttttaa agactcccgc atctcagcca cacggacaag
     6301 ataacgatca taacaatccc cgtggagccc tattggaatc tcaaagtcaa gatcactata
     6361 aacctcataa ggctgtgccc ggcgcaaatc ccacggcaca ccactggcac gaagcatggg
     6421 ccctgtgaag ccccattcaa tggcttgatc tttgctcacc acaccaatat ccacagtgcg
     6481 ctgtttaaaa atgcgattat gagtgacaac attttccata tcctccacca tggaaggaaa
     6541 cgtctcacaa aaaagaccga tctcctctag caggctttcg ggtaaatcct gatgcacgcc
     6601 ccccggccta aagtaagccg catgaaagcg cgcaccacag gcttgctcat agaaattgag
     6661 aattttttcc cgctcctcaa aggcccataa aaacggggta agcccgccta cgtcaataat
     6721 aaacgttgta atgttaagaa ggtgattcgc aatccgggtc agttctgaaa aaagcacacg
     6781 gatatattga gcccgaaggg gaatctcaag agccaacagc ttttcaatag cgagcacaaa
     6841 ggcatgttct tggttcatcg gcgatacata atcaagacga tcaaaatacg gcaacgcctg
     6901 caaatatgtt ttatgctcaa taagtttttc tgtgcctcta tgcaaaaaac caatatgggg
     6961 atccgcacgc tcaaccacct cgccgtcaag ctcaagaaca aggcggagca cgccatgggt
     7021 cgcggggtgt tgaggaccaa agttcagcgt atagttttgc ttgtcttctt tttgctgact
     7081 catgtcaaag actcctttcc atgctttgca cgagtctctt gtgctacgtc aactgctttt
     7141 tcgtcaccgg gaagcgtata ccctcccaaa gctccttcga gcattccctc ccaaggactt
     7201 attgcatcaa aaacacgaaa agcttggggt aaaatgacgg gctcatagac tactttttga
     7261 gcaatatcgt catagcgcac ctcgtaataa cctgtgagag gaaaatcttt gcgcaaaggg
     7321 tgcccctcaa aattatagtc cgtcagaata cgcctaagat cgggatgccc tgaaaatgta
     7381 atcccaaaaa gatcaaaaac ctcacgctcg taccaatttg ctgcctcaaa aacagaggta
     7441 atcgatggca ctgattctcc atcactcaca gaaactttca tgcgcagacg tttattataa
     7501 tccatgctca aaaaatgata caccatctcg aaacgctttt cacgctcagg ataatccaca
     7561 gccgtgatat caataagctg actaaaaaga cagtcatgat gatcccttaa aaaggtcaca
     7621 caccgcacga gctgatcttt agatgctaaa gttacggcga actcacccaa tgtaaattcg
     7681 cacgccgtac acgcgtcctt caaaagatct gtcacacgca cgtaaaattc ttgatccaca
     7741 gatgtcatca ttgctacgac ctcgcaaaga ctgtgcgatt ttgaattttt tgccttaaaa
     7801 gcaaaatacc ataaagcaat gcctccgccg tgggaggaca cccgggcaca tacacatcca
     7861 caggaataat acgatcacac cccctcacaa ccgaatagga ataatgatag tatcccccgc
     7921 catttgcaca acttcccatc gaaatgacat agcgcggatc aggcatttgg tcataaactt
     7981 tgcgcagtgc tggcgccatt ttattggtaa gggtccccgc tacaatcatc acatctgatt
     8041 gacgcggact tggcctgaaa aaagagccaa accgatcaag gtcatagcga ctcgccgccg
     8101 tatgcatcat ttcaacggcg caacaggcca acccaaaggt catgggccac aaagaattgc
     8161 tctgtgccca tccggcaagc ttatcaaggt ttgtcaaaag aaacccctga tctttgaact
     8221 gctcgcgcat ttcttttgga agggccactg cttgtggagc tgtcattgtt aagtatccta
     8281 cctcaatccg ttttttatct tatcacttat ttagaacaga agaatcctaa aatttcatta
     8341 atcattgcaa aaattttaaa acacttaaat ctaaaatcat tttttctcag cgacccaata
     8401 aaacttaaga caatgaaaac tctaaagttt tgaatgaaac ccaatgtgaa atccaagata
     8461 aaacatcaaa tcattgactg acattttttt cacaggtttt tcaggctcag cctccccatt
     8521 tttattatcc acctgctttt ctttttcaag gatttttggc g
//
LOCUS       DAIAHG010000035         8466 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_35_length_8466_cov_4.210871, whole genome shotgun sequence.
ACCESSION   DAIAHG010000035 DAIAHG010000000
VERSION     DAIAHG010000035.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8466)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35_length_8466_cov_4.210871"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2260 a         1652 c         1849 g         2705 t
ORIGIN      
        1 ccactcggcc acctctcctt atcggatacc tgggaggtta gtcgaaaata acgcaagact
       61 caatattttt ttgagaagat cttctgcatt cttctttgat cttgatttcg agtgttgacc
      121 atcctaaact aagtgcttct ctatatagta aaatttcaaa tgcatgtctg atttttccca
      181 tatccctgtg ttgctttcag aggtcgtcgc ttgtttggcc ccttgtgcgg gtgaaatcta
      241 tgtcgatgca acatttggtg gagggggcta cgcaaaggcg attttagatg gggccaattg
      301 tcggctttta ggcattgatc gtgattttgc agccatcgag cgtgctcaga gctttcaaaa
      361 aaactatgca gatcggtttt tttatcgtca cggtaagttt tcagattttc cttctttctt
      421 agatcaacac ggtattgata aagtggatgg tattattttt gatcttggtg tgtcatccta
      481 tcaaattgat gatccagaaa ggggtttctc ttttcgtttt aatgcgcctt tatcaatggc
      541 gatggggtgt aatgatcttt cggcctatga tgttgttaat cactattcag aaaatgaatt
      601 aatcgcgatt tttaaaaaag gtgaggagcc ttttgcaaag cgtatcgcta aaaaaattgt
      661 ggaaacccgc gtggaaaaac cgattgaaag tacaagtgaa ctcgcggcgc tcattgcatc
      721 atgcaggcca tcgccaaagg atgcgcagca tcctgcaact cttgcgtttc aagcactgcg
      781 tatttttatt aacgaggaac tgatagagat agaaaaagct cttgagcttt gcaaaaaaag
      841 actaaaaccc ggtgggcgtt tgattgttgt cacgtttcat tccttagagg atcgtattgt
      901 caaagatttt atccgcaaag aaagtggtca cacacctttg ccgtctcgtc acatgccctt
      961 tgtggaggct gatttcccaa aacctacatt ttctctaaaa tctaaaaaag cgattattcc
     1021 aacagaggat gaactacgaa aaaatccacg ggcgcgttcg gcaaaactgc gctatgctat
     1081 tcgcgtcggc gattaaaaaa gggagagagt tttttcgtat gttcaagcgt tcaacccttt
     1141 tttttgcctg tgtcgcgatt ggtgtgagta ttgctctttt tcaaattaaa tatcaggtaa
     1201 taacacttga aaaggaatat gagcaaacgc ttttgcggat taaagaaacc caagagtcca
     1261 ttcatgtatt aagagcagag tggaatcacc tcaatgatcc taaacgtttg caggtacttg
     1321 cacaaaaaca tctcaatatt gggccgatta aagcgtcaca attcattgca atgaaggctc
     1381 ttgcaccaag cgatagtgcg tatgataaat atgcgcttga tcagttaatt tctgaggttg
     1441 cagatgtcgt agtaggagat ccatcgtaat gtgttgggtt cttaggggaa ttttttatcg
     1501 aataaatcgc cgaggagagc cctatgccta agcgcctgcc tccttttgca aagaatcttg
     1561 cgtcaaaatc ttttaattca ctcagccatc acctaagtct ttggcatcaa gttgtgcgcg
     1621 atcataatct tttggacaaa gcgcgtaacc gcattgtcgt gggcgtcatt gtctttatgg
     1681 cagcgtttct ttttattacg attcgacttt ttgatattat ggtttttagg aatcatagtg
     1741 agcgtcagca aatcaatgca gagcgctcca gtgagcttgt gctcgagcgt tctgatattt
     1801 tagatcgtca tggcgaggtg ctcgcaaccc accttattac ggcgtctgtt tatgcaaatc
     1861 caaaagtgat tattaatgca cgggaagctg cggagaaact ttgtgcactg attcctgagc
     1921 ttgattttga tacaacgctt gcgcgcctag aatccgaaaa aggctttgtc tggattatgc
     1981 gtcacctgtc gccacggctc caacaaagta ttcttcacct tggtattcct ggaatttatc
     2041 ttcaaaaaga ccagcgccgt gtgtatcctt atggcgctat ggcttgtcat gtgctggggt
     2101 attgcggtct tgataattta gggttatccg gcgttgagaa atattttgat gtgcgtcttc
     2161 gtcaggataa aaatcctctc gttctttcta ttgatattcg tgcacaacat attgttcacg
     2221 atgaactcaa ggccgctatt gaagaatttc aggctcttga gggaaatgcc atgcttttgg
     2281 acctcgattc aggcgaaatt attgcgatgg tgtccttgcc tgattttgac cccaaccacc
     2341 cgaatcaaaa tacaaaagct gcaactttta accgtaatac ccttggcgtg tatgagccag
     2401 ggtcgacctt taagctaatt aatgcggcga ttgcgctcga gaccaatcgt attaagctca
     2461 atacgattta tgatgcaagg caccctataa aagtggggcg ttttacaatt acggattggc
     2521 gccctaagca tgcggggatt attactgtaa gggatgttgt gatgcattct tcaaacattg
     2581 gatcagcgaa aatcgccctt gattttgggt tggcgtggca gcaaaagtat tttgcacgct
     2641 ttgggctgca tacgaaacca aagctcgagc tgcctgaaat tggggcacct attgtgaata
     2701 aaaatcccac agaggcaacg cttattagca actcattcgg gtatggtatt tctgtgtctc
     2761 cccttcaaac ctttcaagcg gttgcagcac ttgtgaatca tggtgagttt cgtcacttga
     2821 cacttttaaa gcaaaaaatg cctgttgtgg gcgcacctat tgtcagcaag gaaacatctg
     2881 cggggattcg caatgtcatg cgtcatgtgg tgacaggcgg ctctggccgc tctgcagagg
     2941 ttgaaggcta tcacgtgatt gggaaaacag gttcagcgca cattgtgcgg ggtaagcggt
     3001 atcacgcaaa cgataaaaca acatctttca tcggggcgtt tcctggtaaa aatccacgct
     3061 atcttctgct tgtaatgttg gattctccca aacctacccc taaaacccat ggattttcaa
     3121 cgggtggatg gaatgcgtgc ccgacagctg gaaaaatgat cgcgcgcctt gcaccaatgc
     3181 ttggcatttc ccctgatttc gattataagg atcccatggg aggggatgtt aaagtgataa
     3241 atcatgtcgc tgattaaatt tttaagcgat ataggtgtcg actcaaaggg gctttctttt
     3301 caaggaagtc agagtaatga caccggacaa ggagggctta tgacatttac tggtatttct
     3361 caagacacgc gccaattgaa aaagggtgaa attttttttg atattatcgg atgttcagat
     3421 cgtgttagat ccgctattca tttaggtgct gcggcgatta tctcacctgt aaaagagacg
     3481 tttaatacgt cgataccctt tatctataca gacaaccccc gtttaatgtt tgcacgcgcc
     3541 tgtgatgttc tttttcaaaa gcagcctgat attattgcgg cgatcactgg gaccaatggt
     3601 aaaacatcaa cggcagattt tatcagacaa ctttatcttt ctttaaaaat acctgcggca
     3661 agtattggaa cacttggagt gaagggagcg cagacatcct ttgagattcc ctcgctgacg
     3721 acattggatg cgtatcatct tcataaagtt ttagcggatc ttgcggagaa aaatattgat
     3781 gcggtggctc ttgaggcgtc gagtcatgga ttagatcagt accgtcttga tggtgtgaga
     3841 gtaaaatatg ccgtttttac aaacctgacg caagatcact tggactatca ccagacgatg
     3901 gatcactatt ttcaagccaa aacccgtctc ttccttgagg tgatggatgc atcgggcatt
     3961 gcgattatta ataaagatga cacgtatggt caagagctgc ttgacctctg tcaggagaaa
     4021 aagatacgca ctttttccta tagcttggag gatgcgagtg cggatttgta tggttctatt
     4081 gagcaaattc acggccatgg gcttcgagtt tcaatcaaag cgtttgatca ccagatcacg
     4141 gtagatattc ctgtggtggg gctttttcaa gttgcaaata ttttggcggc tgtgggcgtt
     4201 atcttggctt caacgccgca taagttatcg gatataaaat ccggccttga aaaacttcaa
     4261 agtccatcgg gtcgtatgga ttatgtgggc aaaagtattt tgggaggatg cgtttttgtc
     4321 gactatgcgc atacaccaga tgcgttgaaa cgtgcgctgc aatctcttcg ggcccacacg
     4381 gccaaccaaa ttcatgtggt ttttggttgc ggtggcgatc gtgatcgttc caagcggttg
     4441 cttatgggag agattgctaa aaaatgggct gaccgtgtga ttgtaacgga tgataatcca
     4501 cggacagagg accctaaaac tattcgacaa gctgttcttc ttgggtgtcc agaggccttg
     4561 gaaaaagcgg atcgtgaaga ggcgatcgcc actgctattg ctgcgttaaa ggagggcgat
     4621 agccttctta ttgcaggtaa aggtcacgag caagggcaaa tgattggcaa ggaaaacaaa
     4681 cctttttgcg atagaaaagt agctttaaaa tatttaaaag gagtttagaa tatgagaaaa
     4741 aaagttttaa ttttgagttg tctgatgttg cacggtgctt tggcaaagga aacacccgat
     4801 gttttcactc ctcagacaat ccttgctgat ggtgtagata gtacggtatc caaaaatcct
     4861 tatacaggcg aagaaggtcc ggcgcgtaag ggaacggtgg ctgcaactgt taataatgtt
     4921 tttcttttga atcaagccct cttagcacca acgccagatg cgtcccacat cgaatctttg
     4981 actcagacga tgcgagcact tgtaccttct cttaatgtaa ttggaatgtt taattttttt
     5041 agtgtggacg agtggctgag tgacaataga caaccgggac gtatcctcgt agcgattttg
     5101 tatttacagc actctcctga aaaagtgaca gaaaccatcc gtcaaaggtt ggatgtcatt
     5161 cggtcaacgg cggctattgc ttttttgacg cgtgagattg atgctctctt agggtgccgt
     5221 taaaaaaaat gaggggcagg ggagagcgta aaaccctctc ccacatccta cgccactagt
     5281 attcttcgcg gatttctgaa aaatggtcat tattctcttt gttattgtta tcatcttcaa
     5341 cgagttcttc gttatccgaa ttgttattca ttatattttt ttagatgagg gttatttttc
     5401 atattttctt ttaggctttc ttcgaggtca tcttcaagga gttcgttata atgatctttt
     5461 ccttcagcta tctctatgat atgatctaat gctttttctt gctgatctag ttgttctaga
     5521 tttttttcaa tattttttac ttcttcactg agatttttgt tatcaatttc aaggttttta
     5581 gatgcatcct tgctgatatt ttttttagca atatccctaa taagatagag tttctttact
     5641 aactcctctc tttccttttg aattttttgt tttttagatg gagcagtagt gagaatgtat
     5701 ttagctctgt tttttatcaa tttttctttc tcttgtgttt ttaactccaa gatctgcgta
     5761 tattcctcta tttttcgggt tatttcgtca atttccccat taatatttag aattttttcc
     5821 tcaagggtca caaaatcttt gattgcctcg tttatgtctt cttcttcttg tgaggaatta
     5881 ttgaaggaat tattgatagt gtgttcctca tctattccgc tttcatcatc actgctccag
     5941 gacggtgtca cttccccgca acatagaagg agagaaagcg caagtgtttt gtaaatattt
     6001 ttcataaatc tatgcctttt gtgaatgaat cacttatgat tataatttat gaaagttaat
     6061 gtaaagtaaa aaatgtataa aataatttac aaatttttat aaataggaaa ttgagtgcaa
     6121 agcgtgatga ctttttcctt cacagaagca atcgtttttg taaaattttc actctcatgg
     6181 ccatgaacgc gcaaagtttc taagacctcc atgatgtatt ctgcaatcaa agtaaattca
     6241 gattccttga agccacgcgt tgtgcaagcg gggctgccaa ggcgaatgcc ggaggtgatg
     6301 agcggtcctt taggatcaaa aggaatacta tttttattgc acgtcatatg cgcttcttcc
     6361 aaagcatttg tagcatcgcg gccattaaga ccaagagggg ttaagtccac caaaaccatg
     6421 tgagagtccg tgccaccact gacaagggaa agccctttgt gtgtcaggac atcgcccata
     6481 gcttttgcat ttttaataac ctgatgggca tattccttaa aatttggatg aagtgcctcg
     6541 ccaaacgcca cagcctttgc ggcaataacg tgcataagtg ggcccccctg aagtccaggg
     6601 aaaacagcag aattaatttt cttagcgata tcctcatcat tgcaaagaat aataccaccg
     6661 cggggcccac gaagggtttt atgtgttgtg gatgtgacaa catgggcatg gggaataggc
     6721 gaggggtaaa caccgccggc aacaaggcct gcaaagtgcg ccatatccac cataagatac
     6781 gcaccgactt catcggcaat cgcacgcatt tttgcaaaat caatcgtgcg cggataggcg
     6841 gatccccccc caataataag cttaggccga tgtttgaggg caagtgcttt aatctcctca
     6901 tagttcatca agtgggtgag gggatccaca ctataactta caggattgaa ccattttcca
     6961 gataaattca caggcgatcc atgggtaaga tgtcctccac aggccaggtc tagccccata
     7021 aaagtatcac caggattgag taatgctaag aacacagcct gatttgcttg agcgcctgag
     7081 tgaggctgaa cattggcaaa agcacaccca aaaagctctt taacccgtgc aatggcaaga
     7141 ctttctaccg cgtcaacata ttcacatccg ttataaaagc gttttcctgg atagccttct
     7201 gcatatttat tcgtcagcac agagccttga gcgcttaaaa cgtcgttgga tacaatattt
     7261 tcagaggcaa tcagctctat ttgattttgc tgacggacaa gttcacggtc aatcattgaa
     7321 aaaatagcag tgtcagagtg catcatgggg gtgccttttt aacggataag ctggctgcat
     7381 tataatcact tcatggttga aagactatat ctttaagggg gtggtagaga ttgatatgtt
     7441 atttggtaat ctacgttttt tagtaaatta tgggattaga atattaatgt taataaaaag
     7501 ttccattttt tgtgaaaaaa atacagaaaa atagtgattt ttaatcaatg agtatcatat
     7561 ttgatagttt ttaagatcaa tgggggaaag tgattttctc tgtaaaaaag ccactttttc
     7621 cgatgaaaca caccgctttt gatgcttttt ttcatgattt tacgcgattt tttcgctttt
     7681 atgggccttt tttgatcgca gtagtgcata tttgagttgt ttcgcatgag aagtacgcct
     7741 tttttgaggt aattttggtg attttaaggg atttttggct gttttaagtc tttgattgtc
     7801 gaaaaaaatc atctatccac caaatacagt gattttttta gcaatattgc gctaatttcg
     7861 cccccattta ttaagatttt cagtattttt tgtattactg aggtgacttt ttcgttttct
     7921 agctaaatca atttttttag gaaaaatcca atgatttttt caccattttg aagctatttt
     7981 cgtggtattt tggtgatttt cggaataatt ttgccacttt gaggctgcga tttttggaaa
     8041 aacacgctgg atcctatgaa aaaagcgatt ttccccacat ttttgcagaa ttttggcgat
     8101 tttcgggata attttgcttt tttgacatga atttttcact tttatgggcg tttacacatt
     8161 atttgagcga ttttttcacc attttgaagc tattttcgtg gtatttttcc actttgaagc
     8221 tataattgtt aggaaaaaca cgctgaatcc tatgaaaaaa gcgattttta ccactttttt
     8281 tcccatcatt gaggcgattt tcgggataat tttgctattt tgacacgaaa tttttcgttt
     8341 ttatacgcgt ttgcacatta tttaagcgat tttttcacga aaaaagtgat ttttcgccca
     8401 tttttctgta tttttcatcg aaattgtgct aattcccctt aaaaacggtc attctccccc
     8461 taattt
//
LOCUS       DAIAHG010000036         8403 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_36_length_8403_cov_3.253063, whole genome shotgun sequence.
ACCESSION   DAIAHG010000036 DAIAHG010000000
VERSION     DAIAHG010000036.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8403)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36_length_8403_cov_3.253063"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2450 a         1591 c         1867 g         2495 t
ORIGIN      
        1 ccgctgccat agttttttta acattgtgcc tttttttcct tttttctttt tataaaataa
       61 tcgtgaagtg cttgaataac agggcgcgcc tctttttctt gagcaacaag agtcatcttc
      121 atctccgata gcccggtaat ggcagcggag aggccaagcg cttggagctc gtgtgtgata
      181 acccttagga cttttgtgct aagatctgta ttctctttaa atccatggcc aataagcgca
      241 attttaacaa cattttgatc tacatcgata tcagaaaaac caatatccga ttgttttttt
      301 ctcaaaaacg taagcgtggg cacaagatct gcttttgtca caaaaaaatc aatatgggcg
      361 gcgtctttct cacctggatg aattgctttt gagagtccgt caaagaggag tccttgctgg
      421 gtgaggattt ggctcatttt tctgagcaca gtatagcttt gaagggaaag ggagcgcaag
      481 gatagacgcg catcgttaaa actatgtgca atgcccgaaa gatcattggc cgcagtggca
      541 taaggagaag tttcaataat ttctgtgcct tcttcggctg tatgaaagct ggaaagaatg
      601 cgtaaacgta cttttttctc aaacgcatag gccacacatt taggttgcaa gacctttgca
      661 ccgtgatcag ccaaaagctg catctcttca aaggtgattt tgtcaatttt ctcagcatcg
      721 ctaattaggc gcggatctgc cgtatacaca cccgaaacat ccgtataaat atcgcaaaaa
      781 tcagcctgaa gggcgtgagc gagcgccaca gccgtcatat ctgatccccc gcggccaagt
      841 gttgtcatgc gcgcgtcatg aatcccttga aaccctgtga caacgggaat gcccccttct
      901 tccataaaat gatgtagagg cgatggatca atggctgtga cggctgcatc tgtgtgttta
      961 gaattcgtat aaatagggat ttgccacccc ataaacgatc ttgatggaat gccaagctct
     1021 tgaagacaaa gacttaagag gccgcttgtg acttgctcac ctgaggaaag aatcacatca
     1081 ctttcctgca atgcttcggg ggttttgagc atagaccctg cagcatgaac atagcttgct
     1141 aactgatttg tcacacctgc catggccgaa acaacggccg tgacacgttt tcctttatcc
     1201 cgtgcgcgtt taataatccc tgcaaccgct cgcaaccgtt ctatatttgc cacagatgtt
     1261 ccaccaaatt tttgaacgac aagcgacatt agcgatttac ggattcgaac aaagagtgtt
     1321 ttaatacatg taatataagc gacagacacg tcctttgaat agaaagattt ttaaatgcat
     1381 gcttcatcca ctgtttttag tgattttgcc ggacagtggt gggacgaaca ggggcccttt
     1441 aagccgctgc accagatgaa tcctttgcgt cttgagttta tcattaacca tatcaaacca
     1501 ttagaaagag agaaccttga ggggattacc cttttggatg tgggctgtgg cggtgggctt
     1561 ttaagtgagc ctctggcgcg ccttggtgca aacgtcacag ggattgattt gtcagaagag
     1621 gcgattgccg ttgctcagaa tcatgcaaag ggagaggggt tgaccattga ctaccgtaat
     1681 gcatctcttg aggcgatgaa aggtgcatct tttgatgtgg tcgttgcgtc agaggtgatt
     1741 gaacatgttg atgacccaaa agaatttgtg aggcagatgg cacgtgtttt aaaacccaag
     1801 ggatgcgtga ttctcacgac ccttaatcgt acgtggaaaa gttatttttt aggtatttgc
     1861 attgctgaaa atattctaaa atgggcgccc aagggagcac acgatcatgc gatgttcatc
     1921 aaaccttcgg agatgaatag gtttttagag gaagctgacc tcaggcttaa aaaactccaa
     1981 ggagttattt ttaatcccct caggtggcag tgggaattta gcgataattt agacgttaac
     2041 tattttgcct tcgctgaaaa aagttaatgc attaaaaact gtataaaaat taaagaaatg
     2101 ccccgatcta tctctgacca catcatgctt ttggccgcgg gccttggcac acgtcttagg
     2161 cccttaacgc ttaccgtgcc taagcctctt gtcaccctag gggacgggac acttttggat
     2221 tatcaccttg gccatataaa agcagcaggg ttttcaaaag ctattattaa cacacattat
     2281 ttagctccgc agattgtcac gcaccttgaa aaacaagcat gtctgcctta tgcactctct
     2341 cacgaggaaa ttcttttagg aagtggcggg gggataggac aagcgcttca ccattttgac
     2401 ggcccttttt ttgttgtgaa cagtgacaca tacacgccaa ataatttgtc gactgtatat
     2461 gcacaaatgc gcacattttt tgatgcagag aaaatggatg ttctccttct tcttacacct
     2521 ttggacaata taagaggata tgaaggtgtg ggggattttt tgtgcagaga taaggacgga
     2581 atgttaacat tgcgcggtga tttgcccagt gcaccttatg tttatttggg gattcaaata
     2641 ctaaagccag agctttttaa aaacagagag ggcgtttttt cgatttttcc tctttggcaa
     2701 aaagcgcaac aggaggggcg gttttttgga ttgattcaca aagattttac gtggttcgat
     2761 atgggcaacc ctcatgggct ttcttttgct agagacaata ttactttaga tcaagtccca
     2821 tgttcacttt tctcaaactc ataggatgat ggaaaggcgt tcaaaagctt ttgaagcttt
     2881 gttgttgaag atattgttcc gtacaaaata aaatcaacca aatcataaaa gttcaacgac
     2941 agatactctc ttgtgtgttc atcatagaat acaagtgctc ggtcaatggt atatgttcct
     3001 ttgtgaattt cacagatatc tggggaaaat gtaaaactta gtaaatactc attttgtttt
     3061 atagtaaact tattgattag agtacccttt tcaagattat tataaatctc cgataaacca
     3121 cagcgtatgt ttatatgctg attggtttct tttgaagcgt cccttttttc tgaaaaaggg
     3181 gaaagatcgg gatgaagatt taggttatct ccagcgaaca aaaaagaaat ttctaaaaag
     3241 gttaggaaca caaaaaatat gaatttcacc tgtgaaaaaa cctttttgaa ggagtgtact
     3301 tagctatccc agcagatgat ttttaaagtg tcaagggact aaagtatttc ttttatgtag
     3361 tgtatcaaat attgattcgc gggcaaaaga agaggtagat tttgctcatc ttttgagacc
     3421 caatggtacc cgccttgatt ttctttaaga acaagatccc ctgaccagtt ttttatatgg
     3481 tagacaagta aaacggcatg atgttctgga taggtatagc ttaaaaaagt caaagggcgc
     3541 atatcctcgc acgcaacatt taaatgcagt tcctcaaaaa gctcacgcgc taaagcctct
     3601 tcgggttttt caccggcttc gattttaccg ccagggaact cgtagtagcc tggataaatt
     3661 ttatgatcag gcctgcgtgt gagaagaatt tttccctctt tgttctcaaa aacggcggag
     3721 gcaacataaa taatttttgt catttttttc tcttattttt cttttagcga atgaacttgt
     3781 aattgtgacg tgataaaaag attgctcttt tgaaggatta acctacaata gcacaggttt
     3841 ttcaagagat gttgcaggat atgatggaaa aattcgcgct gttggctgga aacggcgacc
     3901 ttccaaagca aatcattcat gcactaaaag agcgtggttc tcccttgatc gtagtgtgtt
     3961 ttgagggcat gacgcaggct gaaacatttc aagatcatga tgtattttgg gtgagtatag
     4021 ggcatatcgg cttactgttg aaaacactca aagaccatca cgttacgcac attgtcatgg
     4081 cggggcaaat aacgcgcccc agtcttaaag agctgtcctt ggattggctt ggaacaaagt
     4141 ggcttgcacg ggttgggatg aaggcttttg ggggagatga tgcgcttctc tcaacgctta
     4201 cagagcttat tgaaaaagaa ggctttcagg tcatagcccc ccatcaaatt ctttcaaata
     4261 caacggttgc ctcaggtgtt ttaacaaaaa tttctccaaa taccatggca acagcagata
     4321 tacagcgcgg aagggcgatt ttagatgctc tgagcccttt tgatgtggga caagcgattg
     4381 tcgtgcaaga agggattgtt atcggcattg aggctgctga aggcacagag gcgctgctcg
     4441 cccgtgcaaa aactttgcgc aaacagagtg ctgatggtgg ggtgctcgtt aaaatcgcta
     4501 aaaaaggtca aagtcttctt gtggatgtgc caaccattgg tcctgagacg gtgatgcaag
     4561 tggcgcgtgc aaatctctcg ggtatcgcga tgagtgcgca tacaacacaa attattgata
     4621 aagaaaaagt ggttgatttg tgcaatcacc atcatctttt tcttgtggca atgaatgatt
     4681 gacatattaa agataaaaat ttttatatgt tcgtcgtata cgcataatat ttgttaaaaa
     4741 aatgatgaga aaattctttt ctcttacgct tttacttgct gtttttacag aaactcttat
     4801 ggcttttgag gagattgtcg tccttgagaa gaaaaaagag gaaaataaaa acctctactc
     4861 ttctcaatga aaacttaatg acgatgttgc agatgctctt aaaaaaatcc tgagacgatc
     4921 acaggcgagt ggtcattcat agtggatgga aattttttga taacatccac gttgtgggaa
     4981 aaaggatcga ttagacacgg gtgtatggct tataacttca cggatataaa aggaaatgtg
     5041 acaaaaatag ttcctgaaga gccaacaata aagcttaaag acgtaaaaat ccctttttca
     5101 gaagctttgc gcgatttatg tatagaaaaa ttgttccttg agtgcagatc aggaagggct
     5161 ttttggtcgc aattttgctc ttttaaaaat tttaggatcc gacagacttg atgctgtagc
     5221 taaaaagtgg ttggaaaata atgatcctaa tcagctattt caatattttt ccgaaaacct
     5281 cacggttgat ataaaagaag aaattaaaaa tgttggtatt ttttcctatt ttcctaacat
     5341 tcctctctac acagaatata ggagaaacgt agaagatgcg gcttaccagg gagaaaatgt
     5401 tgtgagcacc gggaagaata aagagggaca gctggagtat ctttgtcttg gattgaatgg
     5461 gaaatttgtg attaaaaccg aagaagaaat tgataaagtt tttgtggatg atcttttaaa
     5521 aagcgagaag tggaaaaaag aatacgaagc gtatgcaaag gaagataagg acacaacacc
     5581 tcttgagtat ttttacaaca cgaaagaatc tgtgaaaaaa tacaataaaa aatgctggga
     5641 aaattctgaa acgttcaaat tgaataaagg tactggcaac ctttgtgaaa aatatatgga
     5701 aatttattgc acaaaagggt tcaaagcgta tcaagcatat tattggaaaa cgcatgaatt
     5761 tgggagccaa tttgatatta caaaaatcaa caagctctta agtctctcta tcgaagagat
     5821 tgaaaaatca gaaagtttcg acacgatttt tgagtaatat taaggtgtgt gtgcccttaa
     5881 gggatgggca cacttttctc ttctagtctc ttctagcatg tacatttttt tgcgtcgaga
     5941 agttcatttc ctttattttg aatcacttca agagccgctt cggctttatt gtgtatattg
     6001 ccttcaaatt ttttctggat aaggtatttt tgatgggcgt caaaatacgc tttatcatcc
     6061 ttaagaaaag cgataagcgt ttcgagttga atttttcgaa tatcttgata gtgaggcatt
     6121 ttgatgccag acgatacatc gcttacacta aaattctcgc gtgaagtacg acctatggtc
     6181 gcggtttctt tgaataaatt atttttataa gagatttttt ggggtgtctt ggcaaggacg
     6241 ctcaagtatc ctgtatccgc ctcccactga ttttgtacat tatctgacat caaaaagact
     6301 aaaaagtctc gcatagagcg cagtttcaaa agcaatttat ctttctcaac actgcttgct
     6361 gtatgatagg catcgagggt gtcgcgataa atccaaaaag caccgccacc agaaatcgat
     6421 gtatagcgtt tgaatttact tgcgctaaat ttggggaaaa ggcccacacg cacatgtttt
     6481 ggatcgagct taataaaagg tacgcgccct gtcccctgag ctaaaaagat cgttttgcct
     6541 tcgttgaaat ttttctctgc gtcatttcct tgatcaagac cgatgatgcc ttgatcataa
     6601 agatttttca tgaaaacaag ccaatccgtc cactgtggat tcgtcagcgt cagttttgga
     6661 ttttgaccac tcaggccatt attttcagag gctatggccg ttccattaat cgcagcccac
     6721 tgatcaaaca gatgaccgtg gagccaacca aacgcgatga tttttttatc aggatacttt
     6781 tgtttgaggt tttttgcgac tttttcaaac tcatcccacg tttttggaaa atgtttatcc
     6841 ttaaaaaacg cagtattcac ataaacgaca accgtgttgg aattaaatgg aatcgctgca
     6901 agacgacctt ttgcgtcact ataaaaacgg cgtagtgtgg gtgttatcct ctccacatca
     6961 agctttagcg cgtccatagg ggatagtttg ttgacctcct cgataaattc ctctgcgcca
     7021 ataagatgtg tattgttttg atcatctgct gttcccattt cgtaatcaaa aacgatgcaa
     7081 ggtggcgtat ttccatcaaa attgttttga atcgccgcat gaatatcctc ataagtgcgt
     7141 ttcgcttcat caaaggaata gagcatgatg ggtgcaggtg cgtttgtatt atactgttca
     7201 ataagtgcat taaaggcatc accaagaggg cccttgaagg ggtggagaag cgaaacactt
     7261 gttgtgtttg catgtacttg attaaggccc cccagaagta gaatgagggc tgcacatatt
     7321 tttttgagca taggggattc cttttttttt agagaataag ttttagatat ggcgagttaa
     7381 aatcaaagac attcattgtc atccataagt gactcacaat gaaaatgcaa agataaaatt
     7441 ctctaatgat tttttgcaaa ctttctttac aaaacattaa aaatcatgca caccttagtt
     7501 taattggatt ttttaagatt ttccccggtc tatgacacat cgatcttcct ctcctttttg
     7561 cctacaagac aaaagagcac tgattacagg cgcatcaggt ggtatcgggt cggcgattgc
     7621 tagggcgttt tatcagcaag ggatcggtca tatggtgctc tcaggaacgc gcacagaggc
     7681 tttggaaaat cttaaaaaag agcttgagaa ctctccaacg tcgcatgaat ctcgccctaa
     7741 gatcactctg atgacgtgcg atttaaatga tgtcacagcc attgaaaatc ttgtgaaaaa
     7801 tgcaacagag tccatggaag gtcttgatat tttggtgaat aatgcaggcg taacccgtga
     7861 taatttgctg atgcggatga aggatgagga ttggcaaaaa gtgatcgata tcaaccttac
     7921 atcatgcttt aaagcgtgca gggcggcgat caaaacaatg atgagcaagc gcgcaggaaa
     7981 aattattaac attgcgtcca tcgtgggcgt cacaggaaat ccaggccaga cgaattattg
     8041 cgcctcaaaa gcagggatga ttggatttag caaggctctt gcccaggaag tcggcagtcg
     8101 tggtgtcacg gtcaactgca ttgcaccggg ctttattgtc tcgaatatga cggagaatct
     8161 gccagagaca gtgaaggaaa aaattctctc tggtattccg atgcagcgga tgggaacggt
     8221 cgatgagatt gcccatgcga ctgtgtttct tgcgagttct gcggggaatt atatcacagg
     8281 tcaaacgctc catgtgaatg gtggcatggc aatgatttaa tgtgcgcgcg attttcgtat
     8341 gtttgagcga taattgtggg tgaaaaccct tttttcggta tattttgatc attttttata
     8401 ata
//
LOCUS       DAIAHG010000037         8391 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_37_length_8391_cov_3.434688, whole genome shotgun sequence.
ACCESSION   DAIAHG010000037 DAIAHG010000000
VERSION     DAIAHG010000037.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37_length_8391_cov_3.434688"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2452 a         1885 c         1591 g         2463 t
ORIGIN      
        1 ttgtgtgcca ttcgctaaat cacgcaagat atattcaccg cgtgccattt catctcccgc
       61 aagaataaga gcttttttcg cccccatttt atccgcacgc ttcatgcgtt ttcctggatt
      121 tcctgtataa atcatttccg cataatagcc attttcacgg agattttgcg ttaaaataaa
      181 ggcctcattt tcaaaagctt catcaaaggg aatcacggca ataatctctg acgttttttg
      241 tggcaatgtg acaagcccca acatacgctc tatcccaaaa gcccaaccaa tccccggtaa
      301 atcaggaccc cccatattcg caacaagacc attataacgt ccgcccgcta aaactgttcc
      361 ttgggccccc aaatgagaag ttgtaaactc aaaagccgta tgattgtaat aatcaagacc
      421 acgtacaaga tgcggattaa gcacatagga aacgccaccg gctgttaatc ctgcgcagac
      481 tctctcaaaa taattttttg aaaaatcgtt taaaaattca ataagctgcg gtgcattttg
      541 attaattttt ttatcactct catctttaga atctaaaata cgcagtggat tcgtcgttaa
      601 gcggcgctga ctatcttcag ataaatcttg cttgtgagac gaaaaatagt caaccaacgc
      661 cctgcgatgg agagcccgac tttctgtatc cccaagcgta ttaagctcaa gagttatttt
      721 atcaatcatc ccaaggtttt taaaaaggtg cgccgccatc gcaatcactt ccacatcact
      781 tagtggatga tccacaccta tgcattcgat gccgaattga tgtaactgac gatagcgaaa
      841 cgcctgtggc ctttcatagc gaaacatcgg cccatggtaa aaaaccttaa ggggaagatt
      901 ttgcgtcatg ccatttgaaa taatcccccg cacaattgcc gctgttcctt cagggcgaag
      961 cgttaaactc tccccacctt tatctgtaaa agtgtacatc tccttgccaa caatgtcaga
     1021 cgtatcccca agtgtttttg taaaaacagc ggtatcttca aaaataggtg tgataaatcc
     1081 tgaataatca taatgccgca cgagagcctc acacacatca acaagacttt tgtattgatc
     1141 gtaatcgacc ccgatccaat cgcgcgtccc ccgaacgggt tgcaatttag ctgacattct
     1201 cacattcctt attttaaaac agaacccatt ctaatctatt tttagggctt ttgccatggc
     1261 ctcggaaaat tccattgcac atcatttcta tcttttttaa actcagcggt gttacctctt
     1321 catcgaaaaa ttctatatgc cgtttttatc ctttgttttt ttattctttt tttctgcttt
     1381 tgcacatggc tcagttaatc ttcaaaaaat tattccttcg tttgaccaaa aactcattgc
     1441 tcttgccaaa gcaaaaggaa tcccagggtt tgctgctgtt attgtctctc aaggtaaacc
     1501 cgtttatacc cagtgctatg gtgtgacgtc tcttgcaacg caaaaaccta ttacccccca
     1561 caccgttttt caattagggt ccgtctcgaa aacaatcaca agcgctcttt tcggtgttct
     1621 ttttcaaaaa ggactcatag caccccaaga caaagtcagt caatatcttc ccaattttaa
     1681 ttaccaaccg ggtgtgctga ccctacatca tttgctgacg cacacatcgg gcatctcaag
     1741 ggctggattc aatgcccttg ttgaggcaga gaaattgaaa cgtgatcaga tttttgaacg
     1801 cttaggccgc agtgaaagtg ccacaatccc gggtacaatg tacgattatc ataatgtggc
     1861 ttatggtctt atcgcaccta tcatagaagc agccatccaa caaagttatg aagacgccct
     1921 tcgcacatat attttcaagc cgcttaacat gcacaacgcc tcagcatcct atgaggggct
     1981 tatggcccaa aaaaatcgtg cataccccca tcaaaaaaca aaatctggag gatacaccgt
     2041 ttgcagccaa taccgcaaag gctattatga aattgctcct gcaggcggtg tgaacgcgtc
     2101 tattacagac atggccgctt ttttagcctc acaaatggga gccgcaccca aaacattaac
     2161 cccacaaact ctgaatatgc tgcacagtcc tttgatccct gcggtggatt tttttacacg
     2221 aaatcctgcc aatcgacaaa aatttaagac ctcttcttat gggatggggt ggcgtgtttt
     2281 agattaccgt ggcactaaaa ttctttttca cggcggatgg gtcaagggct ttagtaatat
     2341 tattgctttt ttaccctccc atcaaatcgg catcgttatt ttacaaaatg cagaaacatc
     2401 ttttcattgg atcgcaacca tgactttgat tgattgtatt cttggcatcg agggcaacca
     2461 ttggggcgaa aagcctggag caccgaaaat tataaaacgc aaaacctcgt tggtaggaag
     2521 aaaagcgccc ttaccttctg tcaaaaaacc attcaagaag gtctcacaac gatctaaggg
     2581 atatcactca tcaacgaatt tggggacgta agaatcaccc tcaaatcttt ttctgaaatc
     2641 ttttttccaa agtacgcaga aaaaaatttt gacccgattg acactgtacc cttttttcct
     2701 cagggaaaaa tttaatttta ttattatatt tttctgaatc gcgaagacat ttctcaaacg
     2761 tatccttgag tactggaaaa aaccaatggg ctaacgatga acgcagcaca attctatcac
     2821 tttttgcgag ctactgtcag gatgtttggc gattcatttg cgataaattt cgcgccatcc
     2881 atatcgtata cgtcaaccat ttcaaaacca ttacgctcta acatatggcg aagttctttt
     2941 tctgtgtaga gttgtagtga aaatgtatta tgcaagcgct ctggtctctc tgcatttttt
     3001 tgaagcataa ttgtatcata gcaagttaaa atacccttct cacgatcaag ggtcgagcac
     3061 tgcgtagaga gaatttgcgt gtcgcctact tttttatgaa taaaatatga gagatcagca
     3121 acactttcat cagtcatagc ctctaggtta agaatatcaa aaatatagat accaccttct
     3181 tttaagttac tgtggatatt gcagagcgct ttttcaaaat cccccctcac aagatggccg
     3241 atggcattga aaatggtgat aacggcatca aaagcagcta cttttagtat acgcatatca
     3301 ccatcaataa aagtaatgtc aagattttcc cttgacgcgc gggctcttgc aatctctaat
     3361 aacccagggc taaaatctgc acctacagta ctatatccac gtttctttaa aaaaaacact
     3421 tgggatcctg taccacacgt aaaatccaaa acggattgaa ccttctgctt tttaagaagt
     3481 ttttcaataa tgctattttt taagtctgta tcctcgttta tgttccaagc atcaaaatac
     3541 tgcggtaatt tttgatactc aagaggaaga ccaagtgcag atgtatccat actttgtgcc
     3601 tataaaataa atttattgta taaaaaaata tcttatttta acttttcctt aattactttc
     3661 atagtatact tacactagca gctagatctc gtagatgcga agtccgcagg ggtattacaa
     3721 atgtagcgat agcttatata aatatgcaag aagagtcatc ttttttatgt gtccttttaa
     3781 ccatcttcca gcccctaaaa aggaagctat gctacgaatc ctccactaaa tcagggggca
     3841 cagtgtcacc catccgagga gaagtatgcc tgcaacaggg tgcaaagaaa tgctgagaaa
     3901 ttttgttaaa attaattttg gatcatccaa ggaaatttta tataaattta gaaaaattat
     3961 tctttattgc gttttgccat tgactaaaga gaggggctat agactaataa cctgcctatc
     4021 cttaatttcc tatgatcagc acatgaatac agaaaaaccc atccatatgt cgcataaacg
     4081 agaaaggacg ggttgaaagg tacgcaaaaa ttagcgcttg gagaattgga agcggcgacg
     4141 tgctttggct tgaccatatt ttttgcgctc aacaacacgt ggatcgcgtg tcaagaaacc
     4201 accttgcttg agaacgggac gaagatctgg ctcaaaaccg ataagggcac ggctgatacc
     4261 atgcttaaga gcacccgctt ggcccgaaag tccaccacct gttacggtgc accacacatc
     4321 gaactgaccc gcacgattgg atgcttggaa aggctgactg ataagcatct gaagcacagg
     4381 acgtgcaaag tacgcagaac cttcacggcc attgacaata attttacctg agcctgcttt
     4441 aatccacaca cgtgcaatgg catttttacg cttacccgtt gcataagagc gaccttgagc
     4501 atcaatctta ggcttcgcag gctcattaac ggttgctgta ccttctgctt ttggagtcgc
     4561 tttgtttttc acagcggctt tgaggtcttc gagaccaact ttttcttgtt cagccatgat
     4621 tagagactcc ttatattttt agaattcaat gatgcaacat ccagcttaac aggattttgt
     4681 gcatcgtgag gatgagcagg acctgcataa actttgagtt tgcgcataat cgcattacgc
     4741 agaggaccac ttgcgatcat acgctcaacg gccttttgaa tgacgcgctc gggatgtttc
     4801 ccttcgagaa tttcaccgat tttgcggctt ttaataccac ccgcatagcc tgtgtgccaa
     4861 aagaactctt tatcctcaag cttattgccg gtaagagcca ctttttctgc attcacaatc
     4921 acaataaaat caccacaatc aacatggggt gtaaaatagg ctttgtgttt accacgcaaa
     4981 cgattagcca cgagtgccgc aagacggccg agaacaagat tctctgcgtc gatgagcgtc
     5041 caaccatgcc ttacatcggc tggtttcata gaacgagttg ttttcataga aataaagact
     5101 ttttaatgtt tgatacgctt tttttagcga aaaacacttc aaaagtcaag ggctatccac
     5161 aaaaaataaa tgtggtacaa taaaaccgca tcaccctgtg ctttgaaaaa cgcacacaga
     5221 ggtctctcat aaaaatcaca aaaaaattgc acagacctgt taaatcttct agtgtttggt
     5281 ttagagacta atctattttt aggttcatca gagcgtatga caaaaaaagc acaccttatt
     5341 ctcgctacat ctgcaatcgt tgcattctca gcgcccacgc tagcagacta tggcatttca
     5401 acatggggac aacttaagta ccctcaaaat ttttctcatt ttgattatgt caatcctgat
     5461 gcacctaaac agggggaatt agtccttggc gctcttggca catttgattc gctcaacccc
     5521 tatattatca aaggaattcc agcctcagga ctgatggaaa caaatgccac tctcctcgct
     5581 cccgcctatg acgaagtgaa cacctcttat gcttatgccg ctagtgatat aaaagtcagt
     5641 gccgataaac tttctgtaac gtttacgctt aatcccaaag cacattttag taatggtgaa
     5701 aaaataacag ccgatgacgt catcttttcc tttaatacgt taaggtcaga gggcgcacct
     5761 atgtataaaa cttattacgg ggatgttgaa aaagttgaag ccataaaagg tcaagatggc
     5821 agtgaacaag tggtctttac acttaaaaac gcgaacaacc gggagcttcc cggaatttta
     5881 gggcaaattc ctgtgctgtc taaaaaattc tacacaacac ataaattttc tgaaacatcg
     5941 ctaacgccac cgccatcctc tggtccttat gaaattgagt cgatcgatcc cggtcattcc
     6001 atcacttatc gccgtgtgaa ggattggtgg ggtgagaatc tcccctcgca aaaaggattg
     6061 cacaatttta ataccattaa atatctctat tttcgtgata gcaacgcctt atttgaagct
     6121 tttaaatcag gacaagtgta ttggcgttat gagaccagtg caaaattgtg gaataccgct
     6181 tatacctttg atgctgtaaa agatggccgc gttaaaatgg aaagcatcga gcattcgatg
     6241 cccacaggaa cccaaggtct tttctttaat acgcgccgtg atgtttttaa aaatattcat
     6301 atccgcaaag ccctcacgct tctttatgat tttgagtggt tgaataagaa tatctttttc
     6361 aatcgctaca agcgcaccct tagcttcttc cctaactcag aatttgcagc cacagcagat
     6421 gcttcacccg aagaaattgc tcttttgaca aaggttggcg ctgatctcaa aaagcatcct
     6481 gagtacgaaa aagcgtttaa gctccctatc tatacagacg aggaagcatc acgagcgatt
     6541 acacaaaagg ctcttaaact ttttgaggag gcaggctata ctatcaaagg acaaaagctt
     6601 gtggataaaa aaggcaagca gttggctttt gaaattctta tttatgatag gagtcttgaa
     6661 cgcatcatta gcccttttgt cgaaacactt aaaggtcttg ggattgatgc aaaaattcgc
     6721 tttatggatg ttgctgctta ccaagaacaa gtggatgatt ctaagttcga tatgatcttt
     6781 agcatctcgc ctgtctcaaa ttctccagga aatgagctcc gtgattggta taaatccagt
     6841 tctgcctcag ccaaaggaac gcgcaaccaa tggggcattc aagaccccgt ggttgatagg
     6901 ctcgtcgaag aggtgatcac agcgaaaaat tttgatgatc ttaaactcaa aatgcgcgca
     6961 ctcgatcgtg ttctgatcaa tggttattat atgattccga cgtggtatac gaataaagtc
     7021 gacgtggctt actggaatgt actggatcac cccaagacac ctcctaagta tatgccgatt
     7081 acacttgaga catggtggtc aaaaggcgaa aagccccagc aggaggtgga atataagaca
     7141 acagaaacga aaacatcgga tcagcctaaa gattccaaag gtaaggacca gacaaatcgc
     7201 actttgtggg atatgatcaa aggatggttt acaaacactc tcaaggcgtg gttcaaataa
     7261 cctaaacatg gctctttaca ttcttaaacg ctttttactc atgatcccga cactgtttgg
     7321 gatcatgctc attaatttta ttattgtaca ggcagcccca ggaggacctg ttcagcaaat
     7381 tcttgctaaa attcgcggtc aatcccttga tacagacagt cgtatcggtg gaacaggggg
     7441 cgatggcatg aatgctcttg gagaccatag catgagcgac cccaacagtt cttatcgcgg
     7501 catacagggg attgacccag agtttattaa acagcttgaa aaacagtttg gttttgacaa
     7561 accggcctat gagcgttttt ttattatgat taaaaattac ctcacctttg actttggaaa
     7621 aagctatttc aaggatgcgc ccgttgtcga tctcgtcaaa caaaaactcc ctgtctctat
     7681 ctcccttggc ctttggacaa tgcttcttgt gtatcttatt tccattcccc ttgggattaa
     7741 aaaagctgtg cgcgatggat ctccctttga tatttggaca agcgttgtag tgattatcgg
     7801 ttatgcagta cccagttttc tttttgcact ttttctgatt attttatttg caggaggaag
     7861 tttttggcaa attttccctt tacggggtct tttttcagat aactttgcaa gtctttcttt
     7921 ttttcataaa ataaccgatt atttttggca catcaccctc ccgataactg ccatggtgat
     7981 tgcaggattt gcaaaactta cattgcttac aaaaaattct ttccttgagg aaattcataa
     8041 acaatatgtt ttatgtgcgc gcgccaaagg aatctctgaa aaagctgtcc tctataaaca
     8101 tatttttaga aatgcaatgt taattgtcat tgccggtttt cctgcagcgt ttattggtat
     8161 tttatttaca tcatcccttt tgatcgagat tttattttcc cttgatggcc ttggtcttct
     8221 tgggttcgaa gctgcaatga gccgtgatta tcccttgatg tttggcacgc tttatatgtt
     8281 cacactcatt ggaatgttcc tccatctcct tggcgattta tcctatatgt ttgtggatcc
     8341 gcgcattgac cttgatcaaa aaaaaggtta ataaacaagt attcgcctca t
//
LOCUS       DAIAHG010000038         8312 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_38_length_8312_cov_3.845659, whole genome shotgun sequence.
ACCESSION   DAIAHG010000038 DAIAHG010000000
VERSION     DAIAHG010000038.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38_length_8312_cov_3.845659"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2635 a         1654 c         1689 g         2334 t
ORIGIN      
        1 ttttaattat tgctgtgggc tttagccaga cgcgttttaa cataacactg acacagatta
       61 tccctaaatt tgagcgcgtt tcacccctca aaggtttttc aaaaattttt ggtaaacagg
      121 ctcttgtcga gtttatgaaa aaccttatta aaatttctgt ggttggtgcg gttatgcttt
      181 tagccctttc aggtgttaat caatcactca tgattatgcc gacactgacc atcggcgagg
      241 gatttaatgt aatgcaatct ctttttgcaa agatttttat cagtgctctt tccattttag
      301 gactcattgc attgctcgac tatatttatc agcgctttca acaccgaaaa cgccttaaaa
      361 tgtcaaaaca agacatcaag gaagaacaca aagaacaaga gggctctccg gaaatcaaag
      421 caaaacttcg tgcgatgcgt ctccaacgta tgcagtcacg cattcaagat aaggtgaaat
      481 ctgctacggt cgtcgttaca aacccaaccc actatgccgt ggcgcttgct tgggacgagg
      541 cgacgatgga agcccctcat gttgtggcca aaggtgtgga tcatctggcg ttgcatattc
      601 gaaagcttgc caacgataac aagattcctc tcgttgaaaa tccacctctt gcgcgtacac
      661 tgtatgacaa ggtggatatt gaccgtgaaa ttgaacccga gcattaccgt gctgttgccg
      721 aagtaattaa atatgttatg gatttgcaaa agaaatggtt taagtaaaaa ttatttttat
      781 tttcaattga ttttataatt ttctcgttat agttttcgaa tgtcgttaat aaaatttaaa
      841 cacaaggagt aaaaaaatga aaaaaataac agcaacattc ttagctttag tgctaggggg
      901 gggtaatttt atttctcatg ccatgggcag tggtgagatt ccaatcactt cctttaagca
      961 ggagcataaa aataacacgg caaagaaaat tgaaacaaaa caagaagaca ttactaatct
     1021 ctcctggggg agtactttca agattcatag cgtaaagata tcctattcgc gtaacatgga
     1081 gttattgact aatgttgagc ccatcaaaat gtcgaattat tacgtctctg gagccatgaa
     1141 ttttgagaag tttatttttg ataccattga gacgaataaa gaaaaacttc tcaacggaga
     1201 tggcatctat gacatagagg atcttaaaat tattccaacc tctgtcactt tttctgatta
     1261 taaatgcagc aaggactcac aatggacggc gttttctgac acaatcgcca aagttattcc
     1321 ttttgacaca tatggagaat atttaggaaa agtcattggg tactaccaaa accagacaaa
     1381 agagggcaaa ggtgttacgc tcaataattt aacgatctct atttacacgg cgcaaagaga
     1441 gtattctgac aaggaaaaag agatcaataa aaaagagaaa gacgaaaaaa acttaatcgc
     1501 aatgataaga gataataaag acacccttct aaaagagaaa aatagtctca taaatgacat
     1561 tacaaagtat acaaaaaaga ttaaggcatt tgagattatc attgagagtc gcctcaatga
     1621 gcttagtctc caagatccag gtagtctgaa tgtagaaaac aagtatttcg attttagcga
     1681 aaacaaagtt gatgacatta acgggtattt aaaattttta aaaattaagg gcactaaaaa
     1741 aacaaataaa gtggatgatg gttttaagga agaaaaatgt gaaaccgatg atgaacttaa
     1801 aaagctcatt gcccttaaag aagttgcttg ctccaagaaa gaagaggcag aagcaaaaag
     1861 agagggtatt ataaaacttt tagataagat tggtcacgtt gatatggatt attaatacag
     1921 atcgaatccg tgcttattag aagacgcctg tcatcgggcg tctttttttt aaacagcatc
     1981 gataattaaa aaattcattc attgtttact tattttgatt tatcctaggg caaacaatag
     2041 acagccattt taaaaccctg tgcatacgat tacggactac attattcgcg aacggtggga
     2101 ggacttcaca accgaagaac atgaccgttg gaagtttttg tttgatcgac agatgaccat
     2161 tttccaaggg cgtgtcgcac aagagcacat cgatagttta aaacgcctta acatttctgc
     2221 ggaccgtatt ccaaaatttg aagagatttc taaaatcctt aaggagcaca caggatttga
     2281 gatcgccgct gtgccgggtc ttgtgccaga ggatattttc tttaaaatgc tgaaaaatcg
     2341 acaattcccg tccacaacgt ttttacgcac tccggataaa atcgattacc ttcaagaacc
     2401 ggatatcttc cacgatattt ttgggcatat ccccttactt gttatccccg cctttgcgga
     2461 ttatatggag gcctttgcca cgattgccct caaagcttta gagcggagtg ctgaggatgt
     2521 tatccgcgct gcacgcattt attggtttac cattgaattt gggcttattc ggcaaaatgg
     2581 cggccttaga acctatggtg cgggtattgt gtcgtcttat aaggaaacga tttactgtgt
     2641 ggaagatcca aagcccattc gcacacaatt tgatctcgca cgggttatgc gcacagacta
     2701 tcgcattgat gatttgcaaa aaaactattt tgtcatcgat tcctacgaag agctttttaa
     2761 tgcgacattg caagatcaca cagcactcct tgacagcctg caaaaactcc cgctgatccc
     2821 tgcggggacg atgatcgagg gggaggaaaa tatccctgcg aatactcctc tctaagttca
     2881 gagagtaagc gttctaatat tttaaaaatg gaccgagaat atcttttaag tatggtcttt
     2941 ttttagggcg gcgaatctct aaccagccaa ggggtgacca ttcgcatatc tcaacatcct
     3001 ttggcagatg tggttttaat atccccaaaa gttttttgcg ttttctaaaa tcattgtttc
     3061 cagcaaaatc aacgatcata tttcccccaa gctgtaatgt ttgaaaaaga gttggaatca
     3121 ttttcgccgc ctcttcattt agaattttca gcgaagagca cgtgccttga ttcacatcaa
     3181 tactgacaag cgtacttgta tattcgatga gcagctggcc gcccgaggga agacttatcc
     3241 aaggagtcat catatctgca agggcttcat aggccccaga gagctcaaaa acatcacttt
     3301 gtttataatg aacaggaggt gtttgaagga taaacgcaaa tgcttttgat gcttggtgaa
     3361 aatcatcaat atgatctgta taaacagcat ccggcgcata cgtgtcgatt aatatttcaa
     3421 gttgtgtgcg ccccttttta atcaaaccca ctgtggggtt ttgagaaaga gaaaaccatt
     3481 cctcatgggt tttgatgagc gcattaaaaa taaaatccgc atcacagctc aaagcttttg
     3541 ttcttaagat aattttacca aaatctttgt tttggataaa agactcaacg tctttgagta
     3601 aaaaatgtcc atcctctggc tctatggagg atgaaaggga tacacccttt tgagagggaa
     3661 aatacataaa ataaggggtg ataagcgcta tctttaagct cagctgtgca ccctttttta
     3721 aaagaccttt tgctgcttcg ccggcgtctg gggtgctttg gcgtttgatt tgcacaaaaa
     3781 atgtatcacc ttctttgagg tgaagaggat tattcaaaac tcctggacga tcaagcccta
     3841 tatcaatcag ggcaaaagct cctaaaactc tttgcacacg gccataaaaa atggattcag
     3901 gtgtatcttt ttcctctaga tcctctgcat caaagaggac aaaatcagcc gcatcttttt
     3961 gaagtgcaag aaggcgaaga ttatgttgac gatggcaaaa aagggttaaa ctcaccttgg
     4021 tgcctaggat tatttttgac gaaacgtaat acgtcccttg ctcaagtcat aaggggtcat
     4081 ttcaacagtc actgtatcgc ccacaagaac acgtatgcgg ttcttacgca tgcgcccgga
     4141 ggtgtgggcc aagatcacgt gaccattttc aagagtaacg cgaaaggttg cattcgggag
     4201 aagttctgtg acgactcctg aaaattcgat aaggtcttct tttgacatgt atttcctaaa
     4261 attagtaatc tcattattct acatgcaacg atttcttgtc cacgtcaagt ttttttccac
     4321 gttatagctc aaagagaaat gatcattctt tgttttttaa agaaaaatta actttatttc
     4381 ttttattatg aatatatatg ggtataattc ttttaaaaaa caaatcgaaa tagccatcag
     4441 gagaacattc atgcgaaccc tctctaggat ttttttagcg acagcaatgc taacggtaag
     4501 cacagacacg cttctctatg cctcagacaa aataacgcct atggatgtca tctatagcta
     4561 tagcaagggt aatcaaaaaa taaccccgga ggatttttta aaaaatgttt taggtttgta
     4621 tggcgtaaaa aagaaaagtc cgacacctga tgaaaaaacc attattttcc gtgctattgc
     4681 agcggggttg aatcaaaaaa atgacccact taccttaaaa aatttaaatg acacgactaa
     4741 agaaatcctg gagtctctta cagaggacaa tgtttctgat atcgctcctc aggcctatag
     4801 catgtatcag gttatcagca gctcaatact caatcatgaa acggccgatg tcacaaaaat
     4861 tgatacaatc atcaaaatgg ctaaaaatga tgcggaaaag ctttttgggg agtatatcga
     4921 catatcccct ctcaaaaatg aaaaagcatc gaatgctgat attgaacgca tttttaatct
     4981 gacagagatc ttaaaaaagg ttccagaatt ttcttttgaa aaatcgatta ccagtgcgga
     5041 ttttaaggtt acaaaagata actttgacga ctatcaagag atgctgggga aaattattga
     5101 tgacagattt gatacgaata cgggtaaagg tgcaaggtac ataaaatcct atgatgagta
     5161 taatgaaatc aaagaatatt ttaaaaatct ccgtgaaaaa gtcaaagaat attgggataa
     5221 cttggagttt gatcccttta caaaagggga acagggatac gtggatgtga atgatcccct
     5281 cgactatcgc cgcattcttg acgctttggg aggtgttttt gaagggcctc ataactataa
     5341 agatattctt aaaagagacg atagcgaatt catcaaaata gatgatttcc tgaaggataa
     5401 acgggagcgc tctacgatta aatatgtgaa gaataaaaaa gttcttggtt tcacggacga
     5461 tacagttgtg tcgcttaccg ttgaaaaagt cttagaaaat ttttcaaaat acaaaaatga
     5521 atcaacctat gataagaaaa agtggggcaa gattgctgat gaaatcaaat catccattgg
     5581 cgaagccttt gttgagaacg cagaaaaatg gagcgattgg cacacccttt ttgggtttgg
     5641 aaatgttttt caaaagtctc tggaaaatct taaaaaatcc tatgataaca atgataagga
     5701 tttctatacg acaaatgata aaaacaacca aaagggtaac ggctctttga tgtttgcggc
     5761 cctctttggg gagaattttg aaaatcctaa ttttgcagcg taccttgccg gaaaaatttc
     5821 tggtctgacc catgggggag atcaagcacg tgcagcctgt gcaggtcttg caagcattgt
     5881 ggcctctctc agacaaggag atgatctgga tacatcaatt gaaagtggtg ttttttatgc
     5941 gtttatattt gaccaaagtg gcgaaacctc tgcgctgatt caaaatgctt atgcattagc
     6001 aaaggaaggt gcagatccta tcatggtgaa tgactatttg cgggggtggg ggagtggtgc
     6061 cgaaacgttg gctgatggtg tttacaaggc tgcgcgcgtt ctccttggaa aagaaaataa
     6121 gaacacatgg tggacgttgt gccgtgatcc aggggacact gattccacaa ctgctgttgc
     6181 cggttccatt attattgcgg caagaaacaa atttaaaatg aaattcccta aagagtggga
     6241 tgacaagatt ctctcgatta aggattttga aaaatattat gataaggcct gggaagaaaa
     6301 gcaaaaaaag aaaaagtatt ttaaaaaatt tattgatgaa aatggcgcga tagatgtgaa
     6361 taaagctaaa aaagtcttta ctgatataaa aaatatgcaa gcggctgttg acgcgtccaa
     6421 tcgactttta aaagaagagg tgccaaacac attaaaaaaa ctttttcaaa acgataagaa
     6481 taaagagtta gctaacacat catttcacat tagcaacgta ttgaaaaaag gagatctata
     6541 tcttcctacg aaagacaatc ttaagcgtcc aacactcaaa gcaaatgtcc aacagatttt
     6601 taaagacctt cttgaggccg ttgaaaatga taaaaatctt caaaacgata aagatgccta
     6661 cacccttctt aaagcacact tagcgtatct ggcgcttttg tgggattagt tctttttccc
     6721 ataatttaaa caagggcaag acaattgtta tctggcttat ttgtgcgcat agggggggcc
     6781 tccccagact tttgaaattg aaataagtga ggaagaaagt gatccgaaaa gtctctgact
     6841 aactcacctt ttaggatgat aggcaagcgc gataaaaatg atacggtgac agagagggta
     6901 gccgctaaga attttgatga caaaaacgtt aaaaaatttg agagcgcaat aagaccggag
     6961 aaggagcgcc tcgaggacta taatgacctc gataattaag ttaaaaaaag gggagccctt
     7021 aggccccctc tattcggaat agtttagtaa tccattccca tgccgcccat accccccatg
     7081 ccaggcgcac ctgccattgg ttgttttggc tcaggctttt ctgcaaccat cgcctctgtg
     7141 gtaatcatca agcttgcaat cgacgcggcg tcttgaagcg ctgtacgcac cacttttgtc
     7201 gggtcgataa taccgaattt taccatgtca ccaaagcgat cattttgcgc atcataacca
     7261 tggctatgat cattgctttc aaggattttg cctacaacaa tggacccatc cgcaccagca
     7321 ttcacagcaa tctgacggca aggtgcagtg agcgcttgac gcacaatttt cacaccgact
     7381 tcttggtcat tattcatgac tttaatacct tctagtgctt taagcccata aagaagggcc
     7441 acaccaccac ccggaacgat accttcctca atggctgcac gtgttgcatg catcgcatct
     7501 tccacgcggt ctttgcgctc tttgacttca acttcggtcg caccgcccac acggatcaca
     7561 gccacaccac cggaaagttt tgcaaggcgt tcttgcaatt tttctttgtc atactccgaa
     7621 gaggattcgt cgatttgtgc acgaatttga ttgcaacgtg cctcaatctc agacttttga
     7681 cctgcgccat ccacaatggt tgtgttatct ttatcgagaa gcacacgctt tgcactgcca
     7741 agcattgcaa tagtcacgct ctcaagttta atgcctacat cctcagagat cacttggccg
     7801 cctgtgacaa tggcaatatc ctcaagcatc gctttgcgac gatcaccaaa gccaggtgct
     7861 ttaaccgccg caactttaag accaccacgc agtttattca caacgagtgt ggcaagggcc
     7921 tcaccctcaa catcttccgc aatgatcaaa agcggacgac cggattgaac aaccgcctca
     7981 agaataggca acatgctttg aaggctggag atttttttct caacaatcaa aatataagga
     8041 ttatccattt ccactgtcat gcgctctgaa ttagtcacaa aatatggcga gatataccca
     8101 cggtcaaatt gcataccctc aacaacatcc aattctgttt gcaaagattt ggcttcttca
     8161 atggtaataa caccttcttt gcccactttc tcaacagctt gggccaacat ctcaccaatt
     8221 tccttttcac cgttggcaga aatggtacca acttgggcaa tttcctctcg tgtggaaacg
     8281 tgcttagacc gttgtttaag atccgcaata ac
//
LOCUS       DAIAHG010000039         8262 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_39_length_8262_cov_3.337813, whole genome shotgun sequence.
ACCESSION   DAIAHG010000039 DAIAHG010000000
VERSION     DAIAHG010000039.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8262)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39_length_8262_cov_3.337813"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    5647..5656
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2370 a         1773 c         1739 g         2370 t
ORIGIN      
        1 cacccaagac ctgaaggata ttactcaatc tattttgaat atgtcgccga aacaacgtaa
       61 tgcccatccg tgcatcggtg ttggtcgctc tgatcttgtc attacaggat cggctatttt
      121 agagggaatt ttgggcgctt ttaatctatc ctggttacgt gtggcggatc gcggtgtgcg
      181 tgaaggtatt ttaatgagtc ttattcaaaa gttttcagat catgcgtcca ctcatgggca
      241 tgaaaaagtc gaggacactc cctccatata gttttttaag agagaaaaat gcgtcactta
      301 aaagtcaaag ttaaaaataa aaaactatca acatcatcga aaaaatggat agagcggcag
      361 ctgaatgatc cttttgttca ccgcgcccgc gccgaaggat ttcggtcccg tgctgcctat
      421 aaaatgcaag aaatccaaga aaaatatcat cttatccaaa aagggatgac cgttgttgac
      481 ttaggagctg cacccggtgg atggtgtcaa gttgtcgcaa gtcttctagg caacacaggt
      541 gctatttatg ccattgatct tttggagatg gatccccttc caggcgtgac gttcatcaaa
      601 ggggatttta atgacattca cacccatatt ccggataaag ttgatgtgat tttgagcgat
      661 atggcgccaa gctcctgcgg gattaaatct gtggatcact tgcgtcttat tggcatgctg
      721 gaggaggttt ttacttttgc aaaagattat ctaaaagcgg aaggatctct tgtggccaaa
      781 gttttacggg gcggaacaga agcgaaattg ttggcggagc tcaaaaaagc atttaaaaaa
      841 gtcacgcact ttaaaccttc atcaagtcgc accgagtcgg ctgaaattta tgttattgcg
      901 caaggatttc gcctctaagc ttttttaaaa ttgctcaaag tctttacttt ttgtaaagat
      961 ttatccctta gaatgaaact catgaaaaat gcttaatgaa agagccatcg cctatgccta
     1021 cttatctttt ggcctccctt cttttaatgt tgacgccttc tgttgtgtat tctacggatg
     1081 agaccgttga tacggatgtg gaatacgacg aagaaaatga agcaaattac gctgttccgt
     1141 ctgaagagga ggtcactgag gaggacattt tttatgaggg ggatgtttat gaggcccaag
     1201 aagacgaagg taatagcgac agcgacgacg acgatgatgc tgcatagctc tcatttttca
     1261 taaaattata atcttttatc acctcaagcc ctcgccaaag agcttaaggt gatttttttg
     1321 ctctcagcag attctttttc aaaaaaaagc atcaatattg caaaattttc tgatattttg
     1381 tttttaaatt atccatccag tcactatttt ttcctctcac cacatggcta aaagaaaaaa
     1441 tgaaaaaaga aggaagattt gaacccgctc ctttgctata ctcgccagag actctaacca
     1501 taataaaaaa tttttatgac acgcgatatt tacgaacgct cccttgacta tcaccgtttt
     1561 ccaaaacccg gcaaactcga agtgaaagct acaaaacctc tcgcagatca acaagatctt
     1621 gttcttgctt attctcccgg cgttgctgca gcaagtcgct taattgatca aaatcctgca
     1681 gaggctgcga atctcacagc gcgttcgaat ctgattgctg tcatcagtaa cggtacggct
     1741 gtgcttggtc ttggcaatat cggccccctc gcggccaaac ctgtgatgga ggggaaagcc
     1801 gttctcttta aaaaatttgc aggtattgac gtttttgata ttgagatcaa tgagacagat
     1861 ccggataagc ttgtagatat tatagccgcc cttgagccga catttggcgg tattaatctc
     1921 gaggatatta aagcgccaga gtgtttctat gtggaagaaa agctgcgtga gcgcatgaac
     1981 atacctgtgt ttcacgatga tcaacatggc acagcaatta tcgtttcagc cgctattctc
     2041 aatgggcttt ttttatgtgg caaaagcatt ggtgatgtta aactcgtcac gtcgggtgcg
     2101 ggtgctgccg ccacagcgtg cgtggatttg ctcgtcagat taggactttc tccggaaaat
     2161 gttatcatgt gtgacagtgc aggcgttgtc cataaaagcc gcacgtttaa agagggagat
     2221 tctaaaaaac gctatgcaac ggacctgccc cttaaaaact taaaagaagc gatggtcggt
     2281 gctgatattt ttctgggtct ttccgtcgcc aatgtcgtta cacaagacat ggttgcggct
     2341 atggctaaaa atccccttgt ccttgctctt gcgaatcctg agcccgagat tcttccccat
     2401 ctgatcaaag aagtgcggga cgatgcgatt attgcaacag ggcgctctga ttatcccaat
     2461 caggtgaata atgttctttg ctttcctttt atctttagag gagcccttga tgtaggggca
     2521 acgaccatta atgatgaaat gaaaattgcg tgcgtcaagg cccttgcaaa tcttgcccaa
     2581 gcggaatcaa gtgatattgt gtcgagcgct tatgggggag aggttcatag ctttggccct
     2641 gaatatatta ttccaaaacc ctttgatccc cgtctgattg tagaggttgc tgcggttgtt
     2701 gctgaagctg cgatggaaac aggtgtcgct acacgtcctc ttgcaagcct tcagacctat
     2761 cgtgatcagt tgatggagta cgtccatcgc tcatccatgg tcatgcgccc cgttatgaca
     2821 gccgcacgtc aaaatcctaa aaaggttatt ttatgcgagg gagaggaaga aaaaatcctt
     2881 cgtgcggctc aaacgcttat tgacgaaggg attgcgaaac ctatccttat cggtcgccct
     2941 tcgattattg aaaaacgcct taagcgcgca ggacttcgtc ttcgccttgg cgtggatgca
     3001 gagcttgttg atccctcaag tgacatacgc ttttcaaatt actggtattg ctatcataca
     3061 aagcttgcgc gcagcggtat ttctcctgat cttgcaaaaa caattgtgcg cacaaactcc
     3121 acagtcattg gcgctcttgc agttgagctt ggcgatgcag atgcccttat ttgtggtctt
     3181 gtggggcgtt ataatgatca cctcaagcat ttaagacaaa ttttaggagg tagaaaagag
     3241 catcaaaccc ttgcagcctt aagtttaatg attttagagc aaggtaatct ttttattaca
     3301 gatccttatg tgaacagcga tccaacggca aaacaaattg cggatattac ccttcttgcc
     3361 gcagagcaaa tcgaacgttt tgggatcaca cccaaggtgg cactcttatc ccactctaac
     3421 tttggaacct ttagcagtct cagtgccatc aaaatgcgcg aagcgttgcg tattttaaga
     3481 gaaaaagccc caggccttga gatcgatggt gagatgcacg ccgacgctgc tcttctaccg
     3541 gatctgcgcg ccaaagtctg tcctgaaaat acactcacag gggaagcgaa ccttcttgtg
     3601 atgccgtgtc ttgattctgc gaacattgcg tttaatctga caaaagtcat tggtcaaagt
     3661 ctggctgtgg ggcctatgct tttaggactt gctaagcctg cgcatattgt gacaccttca
     3721 acaacatcac ggggtattgt gaatctcacc tctcttgctg ttgtcgaagc tcaaggaatg
     3781 gcgcatggga atgaatagca ttttgtgata atgcatttta ttcgcaattg cttcacagat
     3841 ctttttttca tatgcatata gggtatcgcc ttaaaaatat tcatgttttg ccgattcttg
     3901 tgatgatgat catcgcaagc ttgagtgtgt ttaccctttt ttctgccgct aacgctcatt
     3961 tttcaccctg ggcatcaaag caagctatgc gcttttgtat cagccttatt ttgcttatca
     4021 gtgtggcgct tgtggatatt cgcacatggt acacgctgag ttatcctttt tatgttttgg
     4081 cgttcctttt tcttataggc gttgaggtga tgggatttgt gggcatggga gcaaagcgct
     4141 ggctggatct ttatattttt actttgcagc cttctgaact tatgaaaatt gcgcttatcc
     4201 ttgcgcttgc acgctacttt cacgactgcc ctccttctct gattctaaaa aatcgcaccc
     4261 ttcttattcc cattatgctc atcctcgcac cgtcgctgct cgtcatgcgt caacccgatt
     4321 taggaacagc aatgctttta atttttgcag gcacgagcac cctttttctt gcaggagtgc
     4381 aattgtggaa atttatatgt gtggggggcg ctgtcattgg tgcatcgcct cttttatggc
     4441 actttttgca cgattatcaa aaaaatcgca tcctcgtttt ccttaatcca gaaatggatc
     4501 catctcacac aggatatcac gtcatgcaat cgaaaattgc ccttgggtca ggtggatttt
     4561 ggggcaaagg gtttatggaa ggcagtcaag gacatcttaa ttttctcccg gaaaagcaaa
     4621 cggattttat ttttacaatg ttttgcgagg aatttggtat ggcgggcgca cttttccttt
     4681 tgtctctcta tacgatattg attgtttttt tcttcaacgt tgcgcttaaa agtcgtagca
     4741 gctttggaag gttgctggct ttatctctca caatgctttt ctttttttat atttttatta
     4801 atatctccat ggtgatggga ttagttcccg tggtcggcgt gccacttccc ttgatgtcct
     4861 atgggggcac atccatgatc actgtgatgt ttagtttcgg tcttattttt tcaacaagcc
     4921 tttaccgtga tgtacgtttg ggatcaacat aagacgtttt ataaagagaa aaaagaatcc
     4981 ctcctgtaag aaaaacaagc gtcacgccaa gagataatgt acttggaatc tcaataccaa
     5041 gataaaaaag aaaaaccttg cttcctataa atattaaaat cagggcaagg gctttattta
     5101 ggtaataaaa acgattggat aaatggtcca aaataaaata aagagagcgc aatcccaaaa
     5161 tcgcaaaaat gttgctcgta tacacaatat acggatcctg tgtcagcagc aaaacagcag
     5221 gcacactatc cacagcaaaa ataatatccg cacattcaat catcacaagt gccaggaata
     5281 agggtgttgc ataccattta tttttttgtt gtataaaaaa atgctccttg tgaagttttg
     5341 gtgtcaaagg aatatatttt ttaatccaag agatataccg ctcgctcaca tttttctttt
     5401 cttgatcaat ggcacggagc atttttaggc ctgtgattat aagaaaagcg ccaaaaaggt
     5461 aaagaatacc gtgcattttt tcaatgattt caatgcctgc cataatcatt acagcacgca
     5521 gcacgagcac ccctaaaacc ccccaaaaaa gcacacggtg atggagattt ttcggaatac
     5581 atagggcttt aaaaatcact gccatcacaa aaatattatc catggatagg cttttttcaa
     5641 taagatnnnn nnnnnntgtc acataggtaa gagagtttga gaggccgtgt tcttgataga
     5701 tccagagcgc aaagagaagg ccaatagtca cataaaaaag actcatcaag aggctttctt
     5761 tgattgaaat gacacgataa tttttatgga gaacgccaag gtccaggatc aaaagtgtcg
     5821 caatgatggc aataaaaaat ccccaatccc ccatagaatg actcataaaa taacacgtgc
     5881 acattgccct tattttatga gcataccccc cctcttccta agaaaaggtt aaggataaaa
     5941 ttctaaatat aatggcatta aaatcctttt tcttcgcctt gaaacaagcg cttgatattt
     6001 tctttgtgcg tatgaattaa aagaaacgca aggatgatac acaggaaaaa cacataaaaa
     6061 gtacttaaga aataggcata aagcggggag agtataaagg caacgagtgc agataacgat
     6121 gaaatacgca ataacttcgc gacaatcaac catgtaagcg caacgcttaa aaaaagaaag
     6181 ggagagaggg ggagtaaaac cccaagggcc gttgccaccc cctttccccc cttaaaaccc
     6241 agccagatgg gaaaaatatg cccggtaacg gctgcaaagg cacacaaaac gggaacagtg
     6301 gctgagctct ttgtcaccag aagcgcaaaa ataacagcga tggccccctt tgacatatca
     6361 agaagaaggg tcgccgccgc tattttttta tttccagaac gcaacacatt ggtcgcaccg
     6421 atgttgcccg atccaatggt gcgcacatca actccctggc tgtaacgcat gagcaaaaga
     6481 ccaaaaggaa tcgatccgag aagataagat aaaacaacaa gagcgaacgt gagagacata
     6541 ctcaaccttt tattttaatt tttttactaa aaagacccga tgagcgtcag ggaaaaaagc
     6601 ctcaaccacc ttgccatagt ttttatcctc ttttgtataa cacgtgaact caagcttttc
     6661 aaagagctca agcagatctt tatcataccc tggcgcaatg cacgatatac aaacgggtgt
     6721 ttctccggaa ttaggaagtt tatgcccctt attcttcaac tcctgaataa tatcgatcag
     6781 caaacatgca tattcctcta ttgttttttt atgcatagaa tttggatcaa tcgcataggc
     6841 gatggatgca acaccgcctg tgactttcat attattaaag ttagagtcat cctccgtatt
     6901 gattttttta aggacatcta aatcctcaaa aaagttgttc aatccctttt ccacatctgt
     6961 tgtctcatcc ttattcccat aaagaacagg ttggttgccg agattaaaaa agacatgatg
     7021 agtcggtgag ataagaccat aaaacgccgt ggtctcacac cttttgtttg caatatctat
     7081 ataatctgca tttagttgat aaggtttttt gtcctcattc gcaagttttt gttgatgctc
     7141 attcgacttt tctaaaatat cctttaaagc atcttcatct gtgatacttt tgagaataag
     7201 cgtatgctca atattttcat gaacataggt tcgttttccg taaacattac agggatcttt
     7261 tttgtactct atgatctcag aagacgcatt cgcaagggtg ataaaactaa aaaacacccc
     7321 taaaatagac aaaaaatgca caaaaaaaca ctccttatta ttgaataata cttgttaaat
     7381 ttgaaggcag tgggagactg tcactgggtg ccacatccat caaccatgga taagtcgact
     7441 gaaactcttc ataccaaatc gtaagatgtt cgggaacagc ctcgcgcgcc atggagggat
     7501 agcgcaactc taaataagat aaggtcacaa aggaaacaag cgtcgcaaga gtaagtgact
     7561 gatccagtgg ttccagtgac aatgcatcaa gccccgacgt cacggcttga atttggcggt
     7621 tataccaatc ctttgagcgc aatggctcag gcctaaagtg cgtttcatac cgtgcattca
     7681 cagcggcatc cagtatccca tcacaaagcg cttgaacttt caaaatccaa aatttttgat
     7741 cctgaggaat caaagccccc ttgcccatat cattaatata ctcgcaaatc acaggcgaat
     7801 cataaaggac agagccatcc tcaaggatca acacaggaat tttattcagg ggattgaggc
     7861 gacgcagatc ttcatcccgt tgccatgggt gataattgat aatctcgacc gtatcgtaaa
     7921 gattaagcgc tttaagacac accttgactt ttgctccaaa cggcgatgtc ggccaactga
     7981 gtaactttag catgcataga ctcccataga ttagaaagtg cgttccaaac atcatcaggc
     8041 tttaatccct ccatatagca tgtactgcct tgataggaaa aaccaggggt ttgttcgagc
     8101 tcgttaagtt tttgtggaat tctcaaaatt ttattattcg ggccataggg tccatagcgc
     8161 gtatcatcac tcggtccaaa aagcccaagg gtcggaatat tcagtgcggc tgccatatgc
     8221 atgggccctg agtcattacc caagaaaacg tcgcactgac ct
//
LOCUS       DAIAHG010000040         8162 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_40_length_8162_cov_3.252195, whole genome shotgun sequence.
ACCESSION   DAIAHG010000040 DAIAHG010000000
VERSION     DAIAHG010000040.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8162)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40_length_8162_cov_3.252195"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2476 a         1524 c         1890 g         2272 t
ORIGIN      
        1 tcaacgaaaa aagcggcaac gcctaaatca gcgtctgcac catcaaagat ggacccgaag
       61 gatccgtcca catggggaat ggttcaacgc acggctgatt gtccttgtgg cagcggaaag
      121 aaatttaagc attgccatgg cgatttaact aaaaaataaa tgattactgt gttctgtgcg
      181 agctgatcct ttcaaaagag ggggtcagct tttttttata aatattttaa cgttttcttt
      241 atgattttct ttgatgattg ggaggtgatc ctaacgaaat atagtttatg gatgcttgag
      301 gatgaaaaaa ataagcgtat ggctttttat gttttgggga atgatctctg cggctttggg
      361 gagtgatgaa aaaaacattt ctgagaaaaa aaatgatttt acgattgagt ttagcaaagt
      421 tagcaataaa acgacgaatt gtgtggcgac agataatgag ggcaattgga ttggcacatt
      481 tatgtttatt ttgaacccag aagacgaaac tcgggtgttt tatttagcat cagagattaa
      541 taaagcgtac agaggaaaag ggtgggggcc caaaatacaa cgccgatgca ttgatcagtt
      601 gttaaaaaag cgaacagatg cagaaggtgt ttatacgaaa gttgatccta taaactatcc
      661 ctcgataaag aaaaacttag cagcaggaat gactttaaag ggaattgatg attcgggatg
      721 gttctttttt gtggataagc cagagacaaa gtatttggat gattatcttt ttaaggaaaa
      781 tgttccaagt gactatgcaa aaaaagtgta tgaggaattt gagaaagaat atgcaacaga
      841 cggtgatgaa atgggcggtg agtttgagat ctctaaaaaa gctcttgctg aaaaagggat
      901 aaaaaatgcg aaagatttgt ttaacacaat taaaaagatt gccaaagaga acggcattac
      961 atttgaggat tttgagaaag ataaagagtg aaaaatttta cttttctcgg gtgatttttt
     1021 aatgcaaaag ctcgagggca agcttgtgat aaagcgggat gccgaggctg aggttaaagg
     1081 gaaatgtaat cgcgagtgag gccgtgagat aataaccagg gttagctgcg ggaagagcca
     1141 tacgtacggc tgcgggggcg gcaatatagg atgcactggc ggcaagagtg gcgaaaacgg
     1201 tcgctgtgcc aatgctcatg ccacacgcct tggcaaggca cacgccgatg cctccattaa
     1261 tcataggtaa aacaactcct gcaacaataa ggctgagcgg aacacggcgg gactcgacga
     1321 gtttatgtcc ggcgacaagg cccatctcaa gcaaaaaaag cacaagcatt ccttgaaagg
     1381 ggttaacaaa aagtggtgca atgcgttcaa ggcctttttc ccctgaaaga aatccaatca
     1441 caaggccccc aataagaagc aaaatacttt tactcgtcaa aatgcgactg acttcttggg
     1501 cgaggggcaa tgcattattg gtgcgggcga ggaggagggc tataataatg gctggaacct
     1561 cgagaatcgc taagagcgct gtgagatatt tatccgaggg gtagcccatg gtttcaacaa
     1621 aaacctgact cgcaataaaa gtaatcacag aaacagagcc aaagtgtgct gcaagggctg
     1681 ccgcatcaat tttagaatat ttaaaaaagc gcaaactata aaaagccaaa agagggagca
     1741 taaagcttaa aataagcgtc gcaaggagag gacccccaag ggtgctaaga ggtgtgtggg
     1801 aaagcgtaac cccgcccttt ataccgatcg caagaagcag atagattgat aaaatcgtgt
     1861 aaataggttc tgggaactta agatcgcttt tagcaaacgt tgcaaataca ccaaggacaa
     1921 aggcaagcac catgggcgac aaaagattcg tcgtcaaaag atgcgctatc gaaccatcca
     1981 caatgctatt ctcctcaaaa atgcgcctta gttcttgatc gtgagatctt tacttaggcc
     2041 gcttttgcgt gatcactcgt tttgtgtata aggtattttc cagttttgta caaggcctcg
     2101 ttggtttgta aaagcgctaa ctccacatag cctgagtcaa agggttcaat gttcaaatct
     2161 tctgtaagga gggaaacatc atggtaaaaa gcatccattg ttgtgtgcgt ctgcttcgat
     2221 tcaaactcaa taaatgtgcc aaaatctttg aattcatcaa gggcaatctc taatgtatcc
     2281 gtcctatagg tcgtgcgctt tttatccaat aaaacaagtg tgctcaggtt gttttcttct
     2341 aaaaaagctt caaaggtaaa gggattgggt tttgtcaaaa aaatatgccc cgcaaaagct
     2401 ttgttaatgg cctcaaaagt ctgttcagac actgttgaaa aaggaagggg gatattatac
     2461 tcgtggcaaa cgacatgatc accccgggta tttgacgcat cttctttcag gtgctctggg
     2521 ttaaatttta tatccagcga gagatcattg cgtacgcgga tgaaaatacc gtgctgataa
     2581 aacaaaccgt cctttgtgtc aaaataatga tcaacgacgc gcttctcttt ttcaaaggga
     2641 agattttgca gaaatgtttt gatagtgtcc gtgttagtga cacggtattt tttctcaact
     2701 tcgagagggc cttgcataaa aaataatcct ttttgaagag tggatgaatg gaaccattct
     2761 aaagcatttt aaatcacttc tccctcattt ttttaatttt ttttaaaggt tgacagtaaa
     2821 tgatttttac tttttattaa cgtttattcc ctataatgat aaataattaa tgttaactaa
     2881 tggagatatt ctgatgaaaa aaatacttat gctgacctgc tacctgctac ctgctacctg
     2941 ctacctgcta ccaagcttaa cgtatgccag tgaagcaagc aaaaaacgat cctatgacgc
     3001 catagaaagc gatagtgatg atgaaatgac tgagtcaaag cattataaag tgaactctaa
     3061 tgatgaggag atgaaggagc attttgatcc aaaagatatc caatccgaaa aagggtatga
     3121 ttttattaaa aagatcattg agaaaaagtg gaaagataaa aaaaatgaaa gttttaacta
     3181 ccttgagaac aaaaaagatt attcagagat aataaataaa aacaaagaaa catatagtga
     3241 taaatttggt gaggagtttc gtggtgagcg taataaactt atgaaagaac gtaacgaaaa
     3301 agaaaatgag cttctaaaac aagtgatcat aagtgttgtg aatgattata aaatttctcc
     3361 cgatgatttc tttaaggcga atgaggaata tgatacagat aagcataatt ttttcctcaa
     3421 aaatggcgag gtttatggat cagacaaccc attttataat ctgaaaagac tttttaaaaa
     3481 ttagcataaa tttaaagggg gctgtgctct caaaaaacac tgccccttaa ctttttagat
     3541 aactttttaa atagcgccct gtgtagctat ccttgacgtt gacgatctct tcgggcgttc
     3601 ccttcgctac gatgcgacca cctttatagc caccttccgg cccaatatca atgataaaat
     3661 cagcggtttt aatcacatcc aaattgtgct caatcacaag gacggtattg ccttggtcaa
     3721 caaggcggtg gagaacctcg aggagtttgt ggatatcttg gaaatgaagg cccgttgtag
     3781 gctcatcaag aatataaatg gttttacccg tggcccgttt ggagagttct ttggcaagtt
     3841 ttatacgctg tgcttcgccc cccgacaacg ttgtagcctg ctgtccgagg tgaatatagc
     3901 caaggccaac ctcttggagt gtttgtaatt tatcgcgaat gcttgggtgg tctttaaaaa
     3961 agacaagcgc ttcgtccact gtcatatcaa gaatatccgc aatcgattta tctttaaaag
     4021 taatctcgag tgtttcacgg ttatagcgtt tgcccttgca ttgatcgcac tgcacgtaca
     4081 catcgggtaa aaagtgcatt tcaattttaa tgaggccatc cccttcgcag gcctcacagc
     4141 gccctccttt gacgttaaaa gaaaaacgcc ccggcagata accccgtgtt tttgctaagg
     4201 gcaaacccgc aaaccattcg cggacttttg taaagcaatc aatataggta atgggattcg
     4261 agcgcggtgt gcgcccaatg ggggattgat cgatatcaat gactttatca atatattcga
     4321 tgccttcgag ggaatcataa atgcctggaa tatctcgtgc ctcataaaag tggcgggcca
     4381 gtgctttgta gagtgtttca ataacaagag aggattttcc gccacccgag acgcctgtga
     4441 cacaggtcaa tgttccaagg ggaatagtgg ctgtgacgga ttttaagtta ttggctttag
     4501 cgcccttaag tgaaaggtat tgtccaagtt tggctgtgcg acggcgtgag ggaatcggaa
     4561 tttttttctg accactaaga tacgcgcctg tgatactgga tggatttttt aaaagatcgt
     4621 cgggtgagcc ttgaaaaaga atatgccccc cgtgaatacc cgctccgggc cccatatcaa
     4681 tgacatgatc acattcttta atcgcatcag cgtcatgctc gacgacgata acagaattac
     4741 caagatcgcg taggtgtttg agtgtctcaa tcaaacggtc attgtcccgt tggtgaaggc
     4801 cgatcgaggg ttcatcaagc acataaagaa cacctgttaa accagaacca atttgcgagg
     4861 caaggcgtat gcgttggctt tctccgcctg agagtgtgcc ggatgcgcgt gcaagagtga
     4921 gataatcaag gccaacatta ttaagaaaat gcaggcgatt atgaatctcg tttaaaatac
     4981 gatccgcaat atctttttct ttttgcgtta gatgcgttgg aatatctctc atccattgat
     5041 acgcgttctc aatcgacatt tctgtgatat gaccaatgtg ctttccatta attttaacag
     5101 caagtgcttc tgttttaagg cgatgtccct cgcacgtgcg gcaaatagat gtactttgat
     5161 actgcgtaag ttcttcgcga aattggtctc tatctttatc gaggaatttg cgctcaagat
     5221 tcggaatcac accttcaaag ggtttttgag ttgtgtattt gcgaaaacca tcatcaaagg
     5281 taatgggtaa aatttccccc ttggatccgt aaagaataag atctttaatg tgctcatgca
     5341 gatcctcaaa ggggatatta agggaaaaat ttttgacccg tgcgagagat tctaaaacgt
     5401 atgtataata ttcataagga gatgattttt tcttccctgc ttcagcgcgt gaccaagggg
     5461 caatagcgcc ttctttgagt gaaaggtgcg ggtgggggac aatgcgctct acggaaaact
     5521 gcatgtctac acccagtccg tcgcaggtag gacacgctcc aaaagggcta ttaaaggaaa
     5581 aaaggcgggg ttctatttca ctaatgctga acccagagac agggcaggca aatttactgg
     5641 atagagtgat tgtttcacct gttgctatgt tttctgcgat taataatcca tcggatattt
     5701 taagggctgt ttcaacggac tcggtgagac gtgattgcac gccttcttgg atcacaagac
     5761 gatcgatcac gacaaaaata tcgtgttttt tgtttttatc aagggcgggt acatcgtcaa
     5821 tctcgaaaag ctcaccgtcg acttttatgc gggtaaagcc cttttggcgg agatcaagga
     5881 attccttttt atactcaccc ttgcgccctt gagcaatggg ggcaagaatc atcaattttg
     5941 ttttatcctc aagttcgaga aggcggtcta ccatctgact gacggtttgt gcttgaatgg
     6001 gaagccctgt ggcgggggag tggggaatgc caatccgtgc aaaaagaagc cttaaatagt
     6061 cataaatttc tgtgacagtc ccaacagtag agcgtgggtt tttagacgtt gttttttgtt
     6121 caatcgaaat cgcagggctg aggccgtcaa tgctatcaac atcaggcttt tgcataagcc
     6181 caagaaactg tctggcgtag gcggataaac tctctacata acgccgctgt ccctcggcat
     6241 agagtgtgtc aaaggccagt gaggattttc cagaaccact gagtcctgta atcacgacaa
     6301 atctgtctct tggaatatca aggctaatat tttttaaatt atgttcgcgg gcgccacgga
     6361 taataatcga tggggtcata gggtggacaa gcctgatgtt ctagaactct ttatagatta
     6421 cttttaggcg ctttgcgctc aaaaagctag aaaaagagtc gttttatgaa agcatgcctt
     6481 gaaaagagct tgcttttttt ctgaaaatcc tttagaagtg agcctaagta ttcatagtgc
     6541 aaaaagattc cttcgatgaa aaaagatatc catccacaat accacgaaat tacggtcaca
     6601 atgactgatg gcacttcctt taaaacgcgc accacatggg gaaaacctgg tgatacgatg
     6661 caattagaaa gtgatccaaa gtcccaccct gcatggacag gtgccggcca taaattaatg
     6721 gaaggtggac agcttagccg ctttagtaag cgttttggta atttcggcaa tactaaataa
     6781 ttttatttat aaaatttttt tatactttat agatccaggt tttttacctg gatttttttt
     6841 gtcatcttaa gaacttttta agaaatagac ctcatacttt ttgttagtat ggaatagtga
     6901 tgaggtgtcg gatgagcgcg tctgcacaaa gcaaacaaga taaccccatg gatactgggg
     6961 aaaaaagaca gttcctcacg attgtggtcg agaaccagga atatggcatt gatattctct
     7021 ctgtgaaaga aattcgtgga tgggaggaaa caacaccact tcctaatacg caaaattata
     7081 ttcgtggtgt gattcacctg cgtggtgaag ttttaccgat tcttgattta agaagctata
     7141 tagggcttgg cacaacaaca atttcaaagg cgcatgtgat tatggtggtg tcgcttgagg
     7201 gaggaatcgc tgtgggtatt ttagcagatt ctgtctcaga tatgttgagt acctttgaga
     7261 atgatattct ttctgtccaa catgttgatc aaggatgtgg ccccaaagtg attagtggga
     7321 tcgtctgcct tgatagtcgc aatgtagaaa ttttaaatct tcacgccatt ttagaggata
     7381 ttctccccca agatgggaac ctcgatttct ctcaaggagg gaataccgtt ttagaagata
     7441 agattgccgt ttaaatcatt cgaattttta aaataaagga gatttttatg ttttcatttt
     7501 ttaaaaaagg tcaaagtaca gcagaaatgc gctcaaaact tgctgctctt cataaatccc
     7561 aagctgtgat tgagtttaat atggatggga cgattgtgac ggcaaatgag aattttctgg
     7621 gcgctgtggg ctattctctc agtgaaattc agggcaaaca ccatagcatg tttgtggagc
     7681 ctggataccg cgacagccag gattatcgga atttttgggc gagcctgaat cggggggagt
     7741 tccaagctgc ggaatacaaa cgccttggaa agaatgggaa agagatttgg attcaagcct
     7801 cctataatcc tttgcttgac ttgaacggcc gcccttttaa ggttgtgaaa ttcgcaacgg
     7861 atgtgacaaa tgtggcgatt atgcgaattg aaaatgaaaa gggtatcgag gagtcctctc
     7921 gcgtccttgg cgctatggca aacggcgatt taacgcagac actcacttat gattataaag
     7981 gtgattttaa ggcgattaaa gaatcgatta accaaacggt tggaaaactc acatcgacta
     8041 tttacggcat caaagaggct atgtctatta ttaatggcgg cgttcatgat ctttctgagg
     8101 gaagtgagaa tctttcatcc cgcacagaac agcaagcaag caatcttgag gagacagctg
     8161 cg
//
LOCUS       DAIAHG010000041         8082 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_41_length_8082_cov_2.660962, whole genome shotgun sequence.
ACCESSION   DAIAHG010000041 DAIAHG010000000
VERSION     DAIAHG010000041.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41_length_8082_cov_2.660962"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2458 a         1585 c         1693 g         2346 t
ORIGIN      
        1 ttcacgtacg attcttacag cctccacaac ccttcttgct gtcatggctt tgtactttat
       61 gggaggtaat gtgatttctg aatttagctt gcctatttta attggtattc tcattggctc
      121 tttttcatcc atttgccttg cgtcaccttt gttgctttac cttaatatca aacgcaatga
      181 tggaaaaaaa gaagggggga gtgagcatca gcaagaacag gtatcaaaga acattggcta
      241 atacttagtt agaggtcttt ttttctagct tttgcggggg cctaaaaatt ttccgttttg
      301 atctctacaa atgtgggaag agtgatagtg ataaaaagtc gtgatcgtaa tactaaaatt
      361 atagcaacgc ttgggccatc atcgtcgaca tttgagatga ttgagtctct ttttctttca
      421 ggcgtggatg tttttcgtct taatttttcc catggtgatc atgcgggaca ccttcaaaac
      481 cttcacttta ttcaggatgt agaaaaaaaa tataaccgcc ccatcggtgt tttagccgat
      541 cttcaagggc ctaaaattcg tattggcgtt attgagaatg ggcctgttac gctaaaatcc
      601 tctgaccttt ttgagctgca tcttcatcct gtgagaggga cagaaaaagc agtgcaactc
      661 tctcatccag agatttttaa tgttgctgaa aaaggaacat ctttgcttct tgatgatggt
      721 aaaataaaac ttactgtgct gaaaaacaaa gagaatattt taacttgtga agtcattgtt
      781 ggcggccctc tctctagtcg aaaaggtgtg aatgttccaa acatacagct tcctctgtca
      841 gccatgacag ataaagatag aaaagatttg gcttttgttc tgacgcaaga tattccttgg
      901 attgcgttgt cttttgttca aacaccgcat gatattgagg aggcacgtgc gcttattggg
      961 acaaaagcta aaattattgc aaagcttgaa aaacctatgg ctcttcggca tttagatgcg
     1021 attgtgaatg caagcgatgc aattatggtt gcccgcggtg atttgggtgt ggagcttcct
     1081 cttgaagagg tgccgagtct tcaaaagcgc attattcgta gctgtcgttc agttggcaag
     1141 cctgtgattg tcgccacaca aatgctagag tctatgattg aggccccttc cccaacccgt
     1201 gcggaggcgt ctgatgtagc aaatgcaatt tatgatcatg tggatgctgt gatgctctcc
     1261 gcggaatctg cgtcgggtca ctatcccaaa gaagcagttc aaatgatgca ccgcattctt
     1321 ttgcatacag aaaaagatag ccaaagcgat tataaagttc tcaaacaagc aacacacaaa
     1381 agtatttctg atgcgattac ggcagcagcg cgcgaaatta cgcagcattt gaatatcagg
     1441 gcgattatca cgcttactca ctcagggagc acggcctatg ctgctgcaaa agaacgcccc
     1501 tatgcgccta ttattggatg tacacaaaat aagcaaacag cacgagaact ttgcctttgc
     1561 tggggggttt atagtctcat tgtgccgcat gatgcagtga atgttgatcc tatttcctat
     1621 gccaaggaac atcactttgt tcataaacat gatcaggtga ttatgacgtc cggtatccct
     1681 tttgaaaagg gcagtacaaa tttccttaag attgtgaatg ttgattagga aaagtatcta
     1741 agcgttaaaa atatctttta cctttgccgc aagatcctta aggctaaagg gttttgcaag
     1801 aaaatggatc tttgcatcat gccctaaatt ttttcgaaac gtatcttctg tatagccaga
     1861 aataaaaata gttttgagct caggatagtg atctttaatt tttttagaaa gtgtaggccc
     1921 atccattttc ggcatgacaa catccgtaat cagaaggtca aaacgctctc catttttgac
     1981 aagatcaagg gcttgatcac cacttgatgt ttcggtgacg ttatatccct tttcacgcaa
     2041 agcgcgagca ctaaaaagcc ttacagcatc ttcatcctca acaagaagaa ttttcccacc
     2101 acccgttaag tctgttgttg gggtttctgg aacaacaaat tcgacaatct cttcgccttg
     2161 aaagcgcgga agaaaaattt taaaaatcgt acccgtggga ggggttattg agctattaac
     2221 ggcaatgaat ccacctgttt gcttgacaat accgtacact gttgaaaggc caagccctgt
     2281 tcctgcgccg acttcttttg tggtaaaaaa aggttcaaaa atacgttcaa tattctcagg
     2341 ctcaatgccg tggcccgtat cttgaagttc gatcaaaaca taatctccct ttggcatcat
     2401 atcatggcca aggcgtgtga cctttgaact gtgataatta gaggttttga tgattaattt
     2461 accctcttgc cccatcgcat ctcgtgcatt gacaacaagg ttaataatca cttgttcaag
     2521 ttgacttgca tccactttga taggccaaag ctcgcggcca tgggtcattt gaagatcaat
     2581 ccctgcacca ataagacggc gtaagagggc tgtgagttct gccaatacat ctgtgatatg
     2641 agtaatttta ggttgcagtg tttgttgacg tgaaaatgcc agaagttgac gcactaaatt
     2701 tgctgcacga atgccatttt gcttaatctg cataacatcg ctataggaag gatcgctggg
     2761 gatacagcgc tgaagcaaaa gatcacaaaa gcccagcatc gctgtgagga gattgttaaa
     2821 gtcatgggcg atgcctcctg ctaattgccc cacggcttgc attttttgtg attgaataaa
     2881 ttgctgttcg aggcgttttt gctcagaaat atcaataaat tgaaggagag ctgtatcctt
     2941 atcgatgcgt gaagcatagg ccgtagtgtg aattttttca tcactaaaac gtacttcaaa
     3001 gggctgttga ttgtgcatag aatcaaaaag tttttggatt ttcttatgaa tatcactgcg
     3061 ttgaccttca ttaacaatat ctgtaaagac atggaggtca ttaatctcga ggtattcgac
     3121 gatcatatga cgaaaagctg cgttcatcgc gatcacgtgt cctgccttgt ttaattttaa
     3181 agcaggcagc gcttcttttt caaaagtatc aagatagcta cccgccacac ttgccaaaga
     3241 ttgaacaggc atacgaaaaa caagattcgc cgatccattg agaatatgat aaagcttcaa
     3301 aggaggatct gtatgaaggg tgacccaacc atcattcgat tgatttaaaa tatcactgac
     3361 acgcctacca ataagctctt ctttaagctt gccaaaccaa ttagcaaaag ttgcattaag
     3421 ccccacaagg tgaccgctac gacttgtgta ataaagacca aaaggggcac tatcaataaa
     3481 tttttcaagc gcattataac tttgttggag ttgattaaaa ttgcctacag gctgactcac
     3541 atcaacaaca gcaacgccta aggatttttg gtgagggaaa actcgaacca tccaccattt
     3601 ttcgaactgt ccaagaccat tgccgccgcc atttacaagg acatcacaat attgttgagt
     3661 ttccaacgct tcttggagtt tcgcagacgc ctcactgggt tgaatttttt gaaaaagctt
     3721 acgtgcaaaa tctgacaaat taaggtaagc aggtcctttc gtactaagct gtagctgccc
     3781 ttttgtatca aaaagagcga cctcacacat gctcgcaata tgctgaaaag cgagggggta
     3841 ataaatattt tttttatccg tccgttgccg gttcaaaaaa gcggcaagaa taaaaataag
     3901 aagggcaaga cttccaagag ccataaattg gagaggcgtt gagattgaga gagagagtag
     3961 cgcaaaagag agaataaata aaataatgcc tacaaacaca cccatacggc cgatcattcc
     4021 cataaaaacg ctttatccaa ttttcctgtt atcttctcca agaatgcccg aaataccgtg
     4081 gtgatgctat gaaaaaaggc gtttaacgtc tttagcttgg gcatgagagg ttaataaaaa
     4141 ataatcatat tgaatttcta ttattttatt agcataaaat caatctaacc aatttaaaaa
     4201 agtaatttta attttatgaa aacaatagca ttattaagcc tttcttcttt tttaatgaca
     4261 acggcttttg cctctgaaaa atctgaaaaa ctagacatca actcaggaga tgtaggaaat
     4321 ttagttgctt ttcctcaaac gcttgagaaa gaaaatgaag agatagagaa agccgcaaag
     4381 atactgatta agtttatcaa cagttgtact gccaagatcg agacacaatc ctctgatttt
     4441 aaaaagtcga agattgtgaa aaaaaagaaa acgaaaaaat gcttaaaaag agataaagcg
     4501 tttgatctta agatcttcag agaaacggaa gaaaatcaaa taaatttttt taaggatgat
     4561 atcgattatc tcaaaaattt tattgttgag aaaagttctg acaaagaaaa agctaaaaaa
     4621 ataacggata aagatgcatt agagtggatg aagaaaacac aacaagaggc cttgaatgat
     4681 attaatgagc ggtataagac tgatgtatcc tactctcttt ttcgtcatta tttagccaaa
     4741 gactttcaat atgaatggcc aagtccctcc ttcaaaagac ataccaaaaa agataaaaaa
     4801 agatctagca actgattctt gccgtgagag ggcagacatg tctaaaatag aactataata
     4861 tttttaagaa tgacttacta tgacccagca accttacgtt attgttttag ggaatgaaaa
     4921 aggagggaca ggaaaatcca ctgtgtccat gcatcttatt acaagccttc ttcgggatgg
     4981 ttatactgtg ggctctgtgg atgttgatgc acggcaagga acattgacgc ggtatgtgga
     5041 aaatcgtagc ctgcgcaaag cagcaaagga acaagatctt cctttgccgg tgcataaacc
     5101 tctttttaaa agtgatcttg tcgatcgtca tgaggcagag gcggatgaag ccgcacgctt
     5161 tgagggagta atgacggcat taaaggatat ggactatatc gttattgata cgcccggaag
     5221 tgatagttac ttgtccaggc tcgcccattc ttttgcggat acactgatta ccccattgaa
     5281 cgatagtttt atcgaccttg atatgcttgt gcgcttgaat gcggattcac tggatatttt
     5341 aaaaccgagt acctattctg aaatggtgtg ggagcaaaaa aaacaaaaag ccattcgctg
     5401 tggcggttcg atcgattgga ttgtgttgcg caatcgtctt agtagtctta attctcgcaa
     5461 caaagaggaa atggaaaagg tactcacagc acttgcaaag cgtattggtt ttcgctctct
     5521 tcctggtttt ggtgagcgtg tcatctttcg tgagcttttt ttgagtggtc tcacactttt
     5581 ggatatgcgg gaaaataata tgaccatgag tatgtcccat gttgcggcaa aacaagaact
     5641 tgcctcgttg atgcagatga ttcaattgcc caaaggtcaa aagaaaatgt cttcaagtgt
     5701 ctaaaaaaca taaaatcata tttattttta gttaattatt aaacaataaa tgcttacaat
     5761 aggttttgag agatgttcaa aaaggatttg ttgtatgatg attgttatca taatgagttt
     5821 tttctttttg acgggggatc tttcttttgc gtcgactaaa acgtcggatt ggttttttga
     5881 tatcacggac cattacatta gagcgagtga tctcaatacg cttaaagagg agcttaatca
     5941 gcgacagatc acagacttga accagctgag ttctcgtgag ctctcagaaa tttatttagc
     6001 tcataattct aaaaaaagta caaagacttg gtttttttcg cttttaaacc aatcaattcg
     6061 tgaaaatcaa aaccttgaga aaaatgaaaa aacaaaactt atcaaggaac tctttgttta
     6121 tacaggacaa gaaaacccca cagaggaata tcctctcaag gggtatacag ggatcgataa
     6181 ccctaaagat tggagtgggg ggtgtcttat tttatgaaaa aaggaccccc tccacttttt
     6241 actatttctt tgtaatatct atttttgttg gactggaggg cataaaaccc tttgagtctt
     6301 tctggggaat ttcaggcgtt tttgcggggg cgtggagatc cacagtatcc cactcatgtg
     6361 tttggttatt aaagtgatag aacttaggcg aatcaaactt tgactcatac ttcacaaccg
     6421 caaggcgctc ctcacgatcg gctggtgtga aaagcccaag accaatcatc cccataaaat
     6481 caatatattt aacggtaaag tatgtttttt tcataagcgt ggggataagc tcctctaaat
     6541 cttttaagga cttaagggcc tttccatcca tttctgtcat ttgaacaatg cgcgtatcaa
     6601 ggaaaaaggg gggaagatct ccattaaaaa gagtacgaaa cggagaggtt tgccctacac
     6661 ccagcataaa aacaccttca ccagcacacc caagaaaaag tttaataaat tcactgtttt
     6721 gaatccatgc agcatcggca aaattaatca tagcttccgc aggtgctaga gaaagtgcat
     6781 aggaatgaat ctgaacatct ttgagctctc ctttgcgata aaaagaaaca acaatgggct
     6841 tgtctgctgg aaaatgattc acaatccggt ccatttcata gagttttgga ccaattaatt
     6901 cgccattgac cttccataga atatccccag gctcaaaagc tttaaacgca tcatatcctg
     6961 gcaggcgtga attaatcata agcactttat tattggcgtc ggggaacgca ttaagatact
     7021 cttgcatggc ttgaacagaa atcaaatcag ccttcaccat atcctcaagg ctcacatatt
     7081 gaggaacgat gccgagtgta cggcgcggag gtgttttccc ttgttttaaa aagtccagag
     7141 catccgcaat ataggcgatg gggagtcctg caccggaggt tcctttgccc ccatagacaa
     7201 taaggacaac gcggccgttt tcgtcaaaac caggagatcc actggctccg ggtattgtga
     7261 gacccgcaaa ctttaccgat tgctcagcaa aagggccaag catctcataa agcgaaaaaa
     7321 cagtgcactt ataggttgaa aactcatctt tggcagaatt tcccatggtg tacacacttg
     7381 tgttcaactc cagaggttgt ttagacagtt caagcgctat agcatcttta ggcaacttcg
     7441 atttttcaat ttttagaaac gcaaaatcaa aaagggggtc aaaataaagc aacttagctg
     7501 ttgttgttgt tccatttgaa aatttaatcg tataagtgct aacggtcata tcgcctgcaa
     7561 catggcgatt ggtagcgata atggcaaaat cctctttggg gtcaatgaaa acgatgaagc
     7621 cggatcctgt ccaagccgaa cgtttttcac tatcagttga ggcgtaagtt ttgacatcta
     7681 cgacaacaac acctcggcgg acaaggtcaa gaattttttt tatattttct gcagtgctca
     7741 gtgcaggttg gatagggggt gtttgaggtg tatcagaggg gggtagaacc tcaggagcgg
     7801 ctgcgtaaag tgcgagacag cttgaaaagc aaaaaagagc aaggacgtta aaaaacataa
     7861 aaaaagtatc tagaaagcat ctggagagag tctactgaat ttttgatttt tttaaaagac
     7921 atcaaaaccg tcaaattatt tctttttctt ggcagccgcg gctttccttg ctgcttctgc
     7981 tgcagctgct tttcttttac gttcggcctc ctcccttttc tttttatcgt cagcagcttt
     8041 cttcgcggcc tgctcccttt tctttttatc gtcagcagct tt
//
LOCUS       DAIAHG010000042         7817 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_42_length_7817_cov_3.463307, whole genome shotgun sequence.
ACCESSION   DAIAHG010000042 DAIAHG010000000
VERSION     DAIAHG010000042.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42_length_7817_cov_3.463307"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2133 a         1550 c         1709 g         2425 t
ORIGIN      
        1 gcgttcgcgg gtggtatgct cagcattttt aatccagggc tgaatatttg tatattgaag
       61 ataggcttca cgtaggtctg gcacgagatc tttaataaca ggcatatgag gaagaggtgt
      121 aatatgcaac ttacctttgt aggtgctaat aaagtgcgta caggcgagtg tgtttgttcc
      181 acatacgttc atagcacagc ttccgcaaac cccctcacga caagaccggc gaaaagttaa
      241 tgttgtgtcg atatggtcct taatatagat gagagcatct aaaaccatcg gcccgcactt
      301 ttcagcattt aatataaacg tatcataacg tggattttca ccaccctcag ggttatagcg
      361 ataaactgtc acttcatagg ttttttcatt gcccgtaagt gtatgatcaa tccactgctt
      421 gcccttgtga atgcgagaat tttccggtag acgaaaagcg accatggttt tcaacatttt
      481 aaataaaatt ttatatataa agcatagcac ttttgtctta aggatctatt aaaatttcaa
      541 aaaagttact cttgaaaaaa tacagataaa aaagtaataa aaaatgcgag ctgcttgcga
      601 agagtgctgg catattcagt caaaatgctt taaaggtttg atgaacaatt tcttcaatta
      661 attatgtata agtttttaag gacaaaatca tgccgtttga ttttgatcag tggacaaaaa
      721 ccattgacac atgtatgcgt aaagtttctg tgccaggggc aacagcgatt ttagcaagtt
      781 ctgagggtat tttacgcaca cacagctatg gctataaaaa tcaccaatcg aaggcatcgc
      841 ctacagcaaa aacacttttt cccatcgcat cgctttcaaa aaattttgca ggtactgttc
      901 ttttaatgat ggtgcaggat gcccatttaa atcttgatga tcctattcaa aaacatcttc
      961 ccgatgtgca aattggcacg aaagatctgt ggggaaagtt aacgtgtgcg gatcttttga
     1021 cacataatag cggctttgat gaatcgattc ttttggagcg ctatgctctg tttcaaaagg
     1081 gtccaaaggc tgtgatcgaa gcgcttaaag attatccaca aagctttccg tataagacaa
     1141 cctattcata ttttaatctc acctacacgg tgattattcc tttgattgaa aaattctatc
     1201 aacttgattg gaaaacattt ttacagacac gcctttttga tcctctcgat tttcaacata
     1261 cgacagtgga tcaagaaacg atgctcagcc gtgatcatag caacctttat gcctttgacg
     1321 cccatggcca ggtgtacgag gacaatttct ataacattcc cgatcaactg acgcttgcgg
     1381 gagggcttta cagtagtgca ggtggccttg gcaaatggat aaaatgtcac ctacagcaag
     1441 gtgcgcctct tttggatgag gattgtttta aaaaaatgta ccacccctat acggcaatta
     1501 atcctattcc tgactatgat caagttcgtt atgctacaga gcgttatcat ttgcatcact
     1561 acggactcgg cttaaaaatc ggttctgtga atggccatgc gctttattat catgaaggag
     1621 ccattaaaaa tgcgcgcagc atccttgtga tcataccctc tcaaaataga ggcgctgcca
     1681 tccttacaaa cataggagat catgatgcag caagtgtggc gatgttttat cttatggatg
     1741 agatgctggg gctgcctgag gttgaccata tccagtatct ttgccctgtg acacgtcagc
     1801 tgaggcaaaa tttgatggat cctttgaagg aacgcgctgc ttaaaatctt aaccaacagg
     1861 tgaataagat cgttgaaaat acgctcttct cttgctttgc gtcgatattc tcgttaaaac
     1921 tagtgacaac gtaaaaaatg aatagagacg attatgccaa aagtaacatt tataaaaccg
     1981 acaggagagg aagtccttat tgatgcgcct ctgggtttat ctcttttaga ggttgcgcat
     2041 caaaataata tagatattga gggtgcctgt gagggatcgc ttgcatgttc aacatgtcat
     2101 gtgattgtgg attcagaatg gtttgatttg ctgcccacag ccacagaaga cgaggaggat
     2161 atgcttgatc tagcttttgg attaacacac acatcgcgcc ttggttgcca aattattatg
     2221 acggatgaac tcgatgggct aaaggtaaaa ttacctgccg ccacacgcaa tatgatggcg
     2281 taaaattaaa gatgcgttgg gttgatacat acacgcttgc gattgagctt gaaaaaaaac
     2341 acaaagatat cgatatcttg gctctgcgct ttacagactt gaaacgctat atcttagcgc
     2401 ttgaggggtt taatgatgat cctgaaaaat ccaacgagaa aatcctggaa gctattcaga
     2461 tggcctggct tgatgaacgt gattaagata gttttgtgag cgattctctc ctttttcaaa
     2521 cgcttcagtt tattacggaa aagcattctt tttcactgaa tacccttaaa aaagcacgtg
     2581 aggatttgac aaagcgctat caaacaaatc gtaccccagg gtttatgggc gaggaagagc
     2641 gtatcgctta tattctagcc cgtcttcctg cgacgtatgc agccgttggc gctgttttaa
     2701 aaaagcttcc agaaaagacg gagattgaaa cggttttaga tcttggctgt ggccctggga
     2761 cagcaactct ggcttgtctg agcgaagaaa ttggcaagaa ctatcatctt tttgagcaag
     2821 atgaaaaaat gctggcgctc gccaaagaat gtcttttgat gcacacgccc ttcgtcacaa
     2881 caacacaaga acgactcgag cacacagaaa attatccaac ggctgacctt gtgattttaa
     2941 gttatgtttt gggggaaata ccctctgaaa aacataaaaa tattctgcaa aaagctttgg
     3001 atgccgcaca gcattttttg atcattatct ctccagggac ttcacttctg tttaaagact
     3061 atcttgcgtg gcgagatttt tttattcaac agggtgtaat gatcatcgcg ccatgtcccc
     3121 atcaaagtgt ctgtcctatg caatcacagg aggggcgttg gtgtcacttc aaggcgcgtg
     3181 tgcagcgcac aaaagaactt aaatttctta aaggtggagc gatgagctat gaggatgagc
     3241 cttataatta ccttgtaatt gcgaaaaaaa atgaaaacaa tcatcaccct ccgcaagcgc
     3301 tcaatcggat tgtaagtcca ccgattcacc gcaagggcca ttctcacttt gaactttgta
     3361 cctttgaaag gacgcttaca cctgttacgc tttccgcaaa agatgatatg tataaaaaaa
     3421 ataaaaaatc tgaatggggc gatgtgattg agagtgctga tgttaaaaag gagggtgaat
     3481 agatgggcaa atggctttta aaatctgagc ccacaacatg gtcttgggcg gatcaaatcc
     3541 gtgaggttac gacaaaatgg gatggtgtgc gcaattatca agcgcgcaat catatgaaaa
     3601 aaatgaatct tggagacctc tgcttttttt atcattctgg cattaaggag cctgccattg
     3661 tggggattgt gacgattatt aagacgtacg aaccagatcc tgtggatcct tcttttgggc
     3721 atgtgaccgt agaagtgtgc gaaacgcttg cttttcccgt gacgcttaaa gcgattaaaa
     3781 tggagccttc attagaaaat cttcctctga tcaaacaatc acgtctttct gtgatgcccg
     3841 ttacggaaag cgagtgggca actcttttga cgatggggag caaagagtga gagaagtctt
     3901 aacctctttt tataagaagc aattcgtctt ctttttattt ttttttatcg ccggtggctg
     3961 cccttttttc accccagtca atgccgctta ttgtaataat tttagctttt gttggtggga
     4021 tagattattg ctcccaaaag cattctattt tcaaaaattt ttctctcagt actgcgcata
     4081 ttcatcgttt ttttctaatt ttcatggggt atggtctact ttcttttttg tggtcgcaaa
     4141 gccctttgct gacgttagaa acatctggaa aattagggat actttggact atcagtctgt
     4201 tcatttcaca ttttttgcaa aaatctcaag atgattatga tggttttttg aaagcacttt
     4261 tttgggggca tatcgccgcc tgccttttca ttcttatgaa tcattttttt gggaatcctt
     4321 tagccatgtg gaaacatgtt tccggggcca aagcccttgt gcagggagga cttttcctca
     4381 gccttagctt ttgggtgatt ttttcttatc ttcttaccaa tatgcctttg acaaaagggc
     4441 gtcttttttt aagaataagt tttacggcgt taaccgctct cacacttgca tgtgttcact
     4501 gcgatacggc tttttacagt cttttttttg gatgcatcat tgctttttta ttctggaaag
     4561 cacaccttcc tgggcttaaa cgtatcgttc aatctctgat tattctggca tttttgacca
     4621 cacctatgat ttgtttttat gcgtttagcc ctcagcattt tcaagtctat aacgaaaaaa
     4681 tgagggatat tagttacctc cacaggcttt ttatttggca tgaaacgtct aagaaaattg
     4741 ctgcacgtcc tcttttgggg catgggctca gtagttataa aacggtggct gacaagggat
     4801 tcgttgacat tgcctatact aacaaaaagg gtgagaaggc cctcgcgcat gtggaaaaaa
     4861 tgggcattca tccccataac attgcgctac agttatggtt tgaacttgga tttttaggcg
     4921 ctgctcttgg aggagcacta acggcctttc tctttggtgt ggtctatgat ataaggaaca
     4981 gtgcaattcg cttttgcggc tttggctttt tttcatcagc catgctgact ttttgggtta
     5041 accttggtgt ttttcaaacg tggtggatcg caagcttgat tatactgatt caccttttaa
     5101 ctttagcacg caaacgatag aacattttat ctaaaaagga taatgattgt atgagtttca
     5161 cgagtttttt tatccaacgc cccgttttat cgagtgtttt gaatgcactt attgttattt
     5221 ttgggttctt gagcctccaa aacattacat ttcgcgaata tcccaatatc gaagtgccta
     5281 ttttgagtgt tgaggttgtc tatcccaacg ccagtgcaga ggttgttgaa tactctgtga
     5341 caaatcctat tgaggatgct ctttcaaaag ttgaggggct tgaggcaatg gcctcatcca
     5401 caaaatatga gcgtgtcgaa attaatcttc agctgaagga aggatcatcc gttgacaggg
     5461 cgatgatttt agtgcgcgat gctctgtccc tcgcgcgcca agatcttccc aaagaaatta
     5521 atgaacccat tgtaaagcgt aatgcagaac gtatgtcagg gcctcctttt atggctattc
     5581 tcattacaag cccaagcaaa ggaatgggag acctttatca ctttgcacaa acatccgtgc
     5641 gtaatggttt tcggagtgtg aagggaattg caggtgttca tctatggggc aatccctata
     5701 caatgaccgt taatattgat cctaagaaaa tgtacgcact gggtgttaat attgatgata
     5761 ttaccaatgc ttttgaacgt aataatcttg cgtttccagc aggaaaatac cgtgatgaag
     5821 tccctgttgt gcttgatctg agcgtaagta cgcccgaaga atttgagaaa attattatta
     5881 aacacaacgg cgagaaaccc atcctcctta gccatattgc gaaagttgtc cttaaagaag
     5941 atacgcgcac atttcgtatg cgctttggtg gagagccgtc tttgatcatg actctttcct
     6001 gcgcgagtga tgcgaatgcc cttgatgttt cccaaagtgt tcagcgtgaa ctcgcccgcg
     6061 tgcaaagtga gcttccagac gatgtgcagc tgaaacttgt gttggatcag gctgatttta
     6121 ttcgagaatc cttgagcaat gtcaaaagtt cgattattga agccattctc cttgtggcct
     6181 tgattatttt tatcttcctg cgcaatgtcc gcgcgagcct tattcctatt gtgacaattc
     6241 ctattgccct tgttggggtt attacaattc tttatggatg tggttattct ttaaatacaa
     6301 caacgctttt agcgcttgtt cttgctgtag ggcttgtggt agatgatgcg attgttgtgc
     6361 tggaaaatat ttaccgttat atcgagcagg gattcagccc tttagaagcg gctcaaaaag
     6421 gaagctcaga aattggtttt gctattcttg cgatgacgat tacgcttgca agtgttttca
     6481 cacctattgc ttttttaagc ggatttacag gaaagctttt gatcgaattt gccgtgaccc
     6541 tttcaggtgc tgtgcttatc tctggtctta cggcattgac cctctcgccc atgatgtgct
     6601 cacgcctttt aaagcatgaa ggtgcgcgtt ttctaccaaa agtcgatgat atgattaata
     6661 ctctccactc acaatatcaa tctgtgctga gtttcctcac gcccaagaaa atcggtcttg
     6721 gtgtcacagc tacacttctt atcttgatca atatttatct cgcgatgcgt ttgccaagcg
     6781 agttaacacc taaggaagac cgtggtcata tttggctttt tagccctgcc actcccggta
     6841 gaaatcttga tgcactggaa aaaatcatga aagatattga aaatcatgtg aatgattatc
     6901 caggtgttgc cagtcgtatg acttttatgg gggattgggg agggacggtt gttctcaatc
     6961 ttgatcccat taaatcacgg aaaaaatcgg ctgctgaaat ggcagatgac cttagaggaa
     7021 tgtttatgaa ctatccttct gtcgatgttt atcccgcaag ttgggatagc ggattaccgg
     7081 accttggcat tgtggaggtg tatggggaat ttggtgcagc ggtcaaaacg attggcacct
     7141 atgcaagtct tgaaaaggat atgcaaggct ttatgatgaa ggcgatggcg tcgggggaat
     7201 ttgcagggtg tttttctgat cttcgcttaa actatccttc ttatgacata aaagttgatg
     7261 aatatgctct gaaccaaatg ggcatatcac cagcacttgt ctcgcaaaca ctttcaacct
     7321 tttttagtgg catacgccag tatcacttta gaaaagatgc cattcgttat gacattacgc
     7381 tgcaaacaaa tgaaaaacct tggaatctta gtgaggttta tataacgggg cctaaagcgc
     7441 atctgatcag tcttgcgagt gtggcaaaaa tggttgcgat ttctgttcca aaatccttaa
     7501 cgcactataa tcaaatgcgc tccgccacat tcaatatgac gatgaagccg ggtgaatcca
     7561 tggcaaaggg catggcggct tttacgcaat taaaggaaaa atatttgcca aaaactttgc
     7621 gcagtgagtg gcttggtgtt gcaaaagttt atgctgattc atcaaacgaa ttactcttac
     7681 ttttagtgat ggggcttgtc tttatctatg ctgtgctggc gattcagttt gaaagcttta
     7741 cggatccctt tattattctt ctcactgtgc cccttgctgc ttttggtgct cttttatttt
     7801 tatgggtgtt tggtcag
//
LOCUS       DAIAHG010000043         7812 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_43_length_7812_cov_3.138591, whole genome shotgun sequence.
ACCESSION   DAIAHG010000043 DAIAHG010000000
VERSION     DAIAHG010000043.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43_length_7812_cov_3.138591"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2217 a         1699 c         1621 g         2275 t
ORIGIN      
        1 tcactacaag ggtaaatttt tcaacgtgca cagccctcgt aaagataaaa atgctcccct
       61 taattccgac taccgcgcct tttttgaaac ggggtttggt aacgtttttg gactaaccga
      121 agattttatt acagaagctc tggatgaaca tcaaaaggcc cttgagcaag aaaaagaaaa
      181 taaaaaaaat tagtataaaa aatttgcatg tgtaggccat aaaatgtggc ttacacacct
      241 cttaactacc ctccaaaact ccatacattt ctcttcagac atcatcccct tctttctcca
      301 aagctgttgc tttttaacat cacatgtttt ttcattctaa aatttttaaa aaatttatat
      361 aaaattatat taatatgccc tctacactga acgggtagta tcaacaaaaa ggatttttcc
      421 gatgaaactt tttaaatata cccttctcct ctcgacactt gctgtagcta ctacggcagc
      481 cgaagcaacc ctctattttg gtcttgccac aggtgcacgc accgttaatt acaattctac
      541 gctaagtgga gatttagata acactttgat attaaccaaa caaaatgccg atgctcaaaa
      601 catcgttggc cgcgtttata tcggcaaaag attttgcctc tcagccccct ggtatgcaga
      661 tgttcaagca acctttgact attcccctat taaagcaagc gttcttttaa caactccctc
      721 agcaggcgtg ccgaatgcgg gtattggtct ttccgttaaa aatacctata cctatggcat
      781 tcgcacaaac attggctata aaataatgga aaacaaacac agtgtgtatg gtatttttgg
      841 gttcaactcc tcccattata aactcacagg caacattgtt tcggcctcca ataaccttca
      901 gtatttcaat ctcggtttag gctatgaaat cccctgcaca gaaagaatga aactacgttt
      961 tgaaggcgaa gtctcccttt acatcaacag aagaatcaaa cacacacctg ctcttacaca
     1021 aatttcgggt gcactcaatg ttaaaaattc caacacattt actgttggct ttgcctatat
     1081 gcttggttaa agaaaatatt ttctaatcac acaccctttg gccttaaaaa gctgaagggt
     1141 tttttttatt tctcatactc tctcacttgc tataaatcca tattttatct atagtaatcg
     1201 gatgcgtact cattttagta aacctttcat ttttactcac aaacgtcccc tcatcacacg
     1261 acctagggcg cggtagagct gttgctttgc cgcatttttc tcaagagaga ttcatttatc
     1321 taattattaa agggtcgttt tatgttgcac acattatcta ataaacaatc aaaagccatt
     1381 ttgggctggt ttctcatcgc actttttttt tgctatcaat atcttttgcg cgttattcca
     1441 ggtgttattt ctgacgaact ccgccatgca ttttacatga cagccgagga tttttcttct
     1501 ctcggatcct attatcttta cgcctattct tttttgcaaa tccctattgg ctttattgtc
     1561 gatcgtattg gcattaaacg cagtgtcctt ttttctcttt ttttatgtat tttaggcacc
     1621 ttctggctta cccaaaccca ttctcttttt gctgcacaga tgagccgtgt ccttgtcggc
     1681 gcaggatcag cctctgcttt tatgggcgct attaaatggg tcgctgatca ttttgaaccc
     1741 ggcagacggg gcactctcat gggggccaca cttgtcatgg gaacctttgg tgcccttggt
     1801 gcaggccatc cactcgtcat gagcgttgag gcttttggat ggaaacaatc cattcttttg
     1861 acatgcattc tcggcgtctt tttagtgata ttcatcgcgc tctttctcaa ggacagtcaa
     1921 aaaaaatcca gcatacactt cgatatgagg caattaaaaa aagaccttaa aaccattgca
     1981 tgcaataaaa caatcgtcat ttatgctctt cttgccatcg gtgtgtacac gccccttgcc
     2041 gttctcgcag acttgtgggg cgtgagtttt cttatgcaaa aattccacat cacgcgcgcg
     2101 gacgcagcaa gtacaaccat gttgatgtat ctagggcttg ctatcggtag cttgacgctc
     2161 cctgcttttg cggaacgtta tcgccttttt acccgcgtta ttcaaatctg cagcctcggt
     2221 cttttagcgc tttttagcgg gatgctgatg gcgcacactc tttctatgct cgcactcaag
     2281 gggattttcc ttctcatcgg ttgcttgtgt ggtgcagaaa tgatttgttt ctcaggcgct
     2341 ctcatcggct cagaaaaacg cacctcgggc cttaccatcg gttttgtcaa caccatgaat
     2401 atgctgggag gtgccgtgct ccaacaactg attggaaccg ccctcgattg gcagtggaat
     2461 ggcgctgttg atcctgcggg cgtgcgcctt tataccatgg agcactacgt atgggccttg
     2521 atgatcctgc ccattgtcct tgtggcatgt gtgttgttat ccatgacgct taagaagaaa
     2581 gattttgatg gctatgttaa gggggctcat tgacaaaaat attttttaat ctaccttcag
     2641 aggtattgag aatgggaaga gatgttttat gatgcttgcg ttatatttta tcgtattttt
     2701 gctgtcgagt tcactaggat cagccaccta tgtaaaaaat aattactctg aaaagggagg
     2761 ggattctttt ctcgatcttt acataaaaga cgttaatcct gatggatttt atcaaaacaa
     2821 accgactaaa acattcgatg agaaaattaa ggaatttaat gaaatggcag agatgttttt
     2881 gtttcttgcc aatgagaaaa gttacctgga ggatggcatt tatccaggta ttgaggagga
     2941 tgaagaggcc acaaaacaag cgaaggatac ttttgattat atgaagagca tgcctctgag
     3001 tgtaattctt tttttgaaga acgtttccct cgaaactagg cgaaagatgc tgacgcaaga
     3061 agaatttgaa aaacttattg aggacaagaa gaaaataaag gaggacgaaa cattacaaaa
     3121 aagcctcaga ggggtttttg atcccgaaga tgatagtgat tttgtgtcat ccatggcgcc
     3181 cgctgtcaaa aagctaaata gcttactgaa ggatggagat gttttgatcg tgcctggaaa
     3241 cacaggtcga tatcttgcca tagcgttaga ggcgtttacg gataagaaaa taacaatcgt
     3301 tcctttagcc atttcgggaa cacccggcat ggagaaaact ggggatgaag catcgtttga
     3361 atgggtgcgc aatattctaa ccccaaaagg tcttgaaaat tatgacaatt acataaaaga
     3421 gcatttgtcc agcacgctaa aaaacctaaa gagtggtcaa aaaatctatt ttctcgatat
     3481 tgtctccggg ggctatggtc tcaatttcct tttagatcgc atcaatacaa tagtgtccga
     3541 ttctgatatt aaacagcctg atattaatct gattaatatt gataagtttt ctcccagtcg
     3601 cctttctaag ggcaaattgc aactccccac atatcttatt gatccgtttg atttgaatgc
     3661 gccagagctt gctcgcgatc tcgataccat tggctatgac gatcgtcttg cgcctgactt
     3721 tccggcgtac ttgtgggatg attgggatgc gaatgatccc tcacaataca caccaacaaa
     3781 aaaagcttta gtttttgcaa aaaatataaa agagtacttc accacactca aagagaagga
     3841 acgcacagat gttgaatgat ttctagtgaa atttttataa aaatattttt ttatcgtact
     3901 acttttctgg tcgaatcctg ataagcacct caacagcatt tgaaaaaata cttatgaacg
     3961 gaacagtagc atcccaatgc tttgtttttt aacgctgagg agatgagccg tgagctttat
     4021 ctcgttcgtg tgtaagaatc ctcgttcaga ggattctttt tgcacctaag gcaatgcttt
     4081 tatttccgct attacatcct cttcacgggg tggatgagca aaaatagtgt ccactgcgaa
     4141 gcctttttta tgcaaaagga aaaagacctc taaatgaaat cgcctgtaaa caccgctttt
     4201 ttgaacatcc ataagggggt cgttttctat gagtaatttt ctgttttcgt caaaatcaaa
     4261 gtctcctcct ttcccaacat agtctgcata gccttctgat acccacaaag gatgacgcat
     4321 aaatgcaaag cgcccaaaac gccatgactc gataatatgc gtcgcttcat gggcaataaa
     4381 ataagaaagc ggtcggtgct cagcgtcagt aagtggcttg gaattgggtg gatgaattgt
     4441 gttcaaagta atatccgatt ctcttatata aatattgcgc gcaagacatg tatccgcaat
     4501 gcctgccatc ttgttactga aatggccata aagccacaaa cgccaggatg cattacaaaa
     4561 aaagattcta agcggccggt ccggctcata aagtgtcgat gttttgaggc gccgtgtcac
     4621 atcatcaagg acatgtgtga tgtctggagg aatggggcga tccgaccaaa ctttataatt
     4681 ttggtaagca atataataat taaagagcgg ctgtggataa atcaaaacac ctaaatacac
     4741 gagaaaaaca gacgcaaatg caattccaaa acgtttgagg tatgccataa tcattctccc
     4801 tttaatttat ttttgaaaaa tctcaaagat tttttctgca cagaaactca ctccttgaga
     4861 gggatcttac aaacatttta cgcgctgagg agggattcta aaatgaataa ttggttgcgg
     4921 gggctggatt tgaaccaacg accttcaggt tatgagcctg acgagctacc gggctgctct
     4981 accccgcgtc aatggtgtat atataggcgg aaagttcttt gaatgcaaga aaaaacgtca
     5041 cagggattaa aaatctggta tactgcgcca acaaaataca aaaacaaagc ggctttttat
     5101 gatcgataaa acctttacgc ctcagacaat tgaaccctct ctctatgaag agtggcttga
     5161 gaacaatatt ttcgcgtgtg atcccacctc agaaaagccg cactacacca tgatgatgcc
     5221 gccgcccaac gttacagggg ccctgcatat tggtcatgcg ctgaatgcga cactccaaga
     5281 tattctggca cggtttaagc ggcaaaaagg ctttgatgtg ttatggcaac ccggaacgga
     5341 tcatgcgggg atcgcgacac aatttgtggt tgagcgcaat cttaaggccc aaggcattca
     5401 tcgcaccgat cttggccgtg aaaaatttct tgaaaaagtc tgggaatgga aagaagaatc
     5461 cggtggcacg attgtgaatc aaatgcagcg ccttgggatt tcgccggatt ggaaacgtgc
     5521 acgttttact atggatgaag gacttagcga tgccgtacgt gatgtgtttg tgaagctcta
     5581 tcgtgacggt cttatttata aagatacacg tcttgtgaat tgggacccat cgtttcaatc
     5641 ggctatttcg gatcttgagg tttttaatcg cgagacaaaa ggaaaaatgt actggattgc
     5701 ttatcccctt gaggaaaatc cggatataaa aattattatt gcaacaacac gcccggaaac
     5761 tatgtttggc gatacggcgg tggctgttca tccagaagat ccgcgctatc aacatcttat
     5821 tggcaaaaaa ttgcgccagc ccctcacagg tcgtctgctt cctattattg cggatgagta
     5881 ctgcgataag gactttgggt cgggcgctgt gaagataacc cccgcccatg attttaatga
     5941 ctttgcgatt ggcaagcgtc atacccttga gtttgttaat atccttaacc ccgatggcac
     6001 tctcaacgac catgtgcctg aagcatttag aggattactt ggtgataaag cacgcagtgc
     6061 aaccgtcaca gccttagaag aatgcggcgt gcttgttaaa accgaagatc acaccctctc
     6121 cattccctat agtgaaaaaa gtgatgcacg ggtggagcct tacctgactg agcaatggtt
     6181 tgtcgctatg gatcagcttg cagagcgtgc gctcaaagcc gtggaagata atcacacgac
     6241 tttttttccc aaacagtggg aaaatacgta ttttgagtgg ctcaaaaata ttcagccctg
     6301 gtgtatctca agacaaatct ggtggggtca tcaaattccc gcgtggtacg gccccgatgg
     6361 cgagattttt attgaaaaaa cccaagagga agccgaagca gcagcgctaa aacactatgg
     6421 ggcgcccact cccctcaccc aagatacaga cgttcttgac acatggtttt cctcggcact
     6481 ttggcccttt tcaacccttg gatggcccga gaaaacaaaa gaactcgaaa aatattaccc
     6541 aacatctgtt cttatcactg ggtttgatat tattttcttt tgggtcgcgc gcatgatgat
     6601 gatagggctt tatactcaag atcaaattcc ctttgaaaaa gtttatattc atgcgcttgt
     6661 gcgcgatgaa aaaggccaaa aaatgtcgaa aactcgcggc aatgttttgg acccccttga
     6721 gctcattgaa aaatatggcg cggatgcgtt gcgctttacc cttgcgattt ccgcagcccc
     6781 cggtcgcgat attaaaattg gtgcgtcaaa ggttgaaaat tatcgcaatt ttctgacaaa
     6841 actttataac gctgcacgct ttttgcttat gcaagacggc gcatcagatc acgctcatac
     6901 tgattttaat ccactcgcag caacacttcc tttaagtaag tggatcatca gccatacaag
     6961 tcgcctagcc caaaatgtac aagatcacct tgacaatttc cgttttgatc ttgcgggtca
     7021 agccatttat cacttcgtgt gggggacata ctgcgacttt ttccttgagt ttatgaaacc
     7081 cattatgaat ggcgacgatg aaaaagcccg cacagaaata ttaaacacag ccttttggtc
     7141 ctttgcacaa atgttaaaaa ttttgcagcc gtttgccccc tttatcacag agcatttatg
     7201 gcgagctgtg ggatataaaa cacttctttg ccaggaaaat tggcctgtgt tcgatgcgaa
     7261 ctatcatgat aaaaacgccg aaagcacgat gaatcacctg atggatctta ttacatcgat
     7321 acgtcgtttg cgtgctgact ataacgtgcc tccggcttct ttcttaaaag tttcagcagg
     7381 aaaatcggat gcgtgtgacg gcatgccctc gcttcttgtc aagcgcatgg cgcgccttga
     7441 tgacattaac tttgatgccc attattctcc caaggcaggc gatattctta ttaccctgga
     7501 cggtgcgtct tattatcttc atttggggga cagcattgat attctccaag aaaaagcccg
     7561 cattcaaaag gacatcgaaa aattagaaaa agatcgtgcc tcgagtcagg ctcgtttgaa
     7621 taatgcggat tttattcaaa aagcaaaaga cgatgtgatc gatgagatga aagaacgcgt
     7681 aagcgcttat acaagcgaca tcgcacaaaa gaaaaaagca ttgattgatt tgggataaat
     7741 cctgctcatt ttattgactc agggagcgat gtgtcctcct tcaaagggtt cattctccct
     7801 ttttctaaga gg
//
LOCUS       DAIAHG010000044         7694 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_44_length_7694_cov_3.462518, whole genome shotgun sequence.
ACCESSION   DAIAHG010000044 DAIAHG010000000
VERSION     DAIAHG010000044.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44_length_7694_cov_3.462518"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    312..321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2221 a         1442 c         1671 g         2350 t
ORIGIN      
        1 gccgatttgc cttcttattt cctggggatt tgtctttttt atcaaagaaa cttatccaca
       61 ctccgatgct aggtcggata aaaaatagag ggtgtttgtt tcttgaaaaa aaagatgcat
      121 tgcgctagaa tgcgtgatac tagcttgttt ttttgaaaga gttctatggc gcgcgttacc
      181 gttgaagatt gtattgaagt tgttcccaat cgttttgatc ttgtgatttt agcagcacga
      241 cgtgcacggg aaatttccgg aggagccagc attacggttt ctcgcgataa cgataaagat
      301 cctgtgattg cnnnnnnnnn ngaactgttg atgctccttc tcttcaggaa aatgttgtgc
      361 gcggcatgca acgtgttggg ttcgttgagg aaaatgattt cgatgaggat atgacaggca
      421 gtgcacatga cgaggcatcg tcctctcttt tcaaagatga tgaagagctt gatgagagtg
      481 atgaagaagc tgacgatgct gatgaggatt tgagtgatga ggacgaagaa gcctaatcgc
      541 aaattttatt atcgctaaaa atggtaaagg gggggctgtg atgccccttt atcgttcaca
      601 ttttagttct tgagcccgtc tgaaaaatct acgatatcgt gattttcttt gagatctttc
      661 atgaacttag ccacaaaaaa ccaactgatg gttaggcttt cattctttgt gttggcctta
      721 ttaacatgat cggtaaaatc cttaagcgga agagctccaa aagatacttt tcccttcaaa
      781 tcaatcgcaa atgctatgga aagttcgcgt atttctacga tgctcatata agcatcgccg
      841 tctcctttgg gtataaatgg aacaccccag cttttatttt tctctcctgt aggcttaaat
      901 tcagagaatg agggtgttgt ataaatttta tctaaatgat tattattctt ttttattgtg
      961 tcagcgatat ctaagagcaa tgaggattga tagagtgaaa aaagtgtata ggttacttga
     1021 cttggatccg aatctccttg aaagactttc atcttttcat aaaccgaatt tgggtttgtg
     1081 ccacggtcaa tatgtgtcag aaaaattttt ccagttttct tattgtgccc tgcaatgctg
     1141 acgcactctt tcgtacctcc tgtatagaga gggcatttgt ctgaaatccc cagtcccttt
     1201 tgtggaatcg gatgaaaatt ctggtaatcc ttgaagggct ctggtgtgct tgaaaggtag
     1261 gtaataatat ttttatattt ttcaaagatt tttgatttct tttgataaag atcatcgttg
     1321 ataatctctg agactatcgt gccgtctgtt attttccgat ggagatcgat ttttgtgcac
     1381 tttttatcaa gaaggaatag caggaaatct tcactcgttt tcaataattt tttcttctcc
     1441 ctttcttttt tgttgttcat ggtgacataa tggatgggca attcttcttt atcctccatg
     1501 ataagtttgt aattcttaat atcttttttt tttcttgcgt gagcgcattt ttctccaacg
     1561 cacacaaagg cgtcataaag gaaaatgtta aaagggataa aaatattttt ctcataaaat
     1621 aaactccatt gtaaattact cctcagatat aaaaaataat ggcagagtat tcaattaccc
     1681 tttgcgcttt taaaaaagtt acacttctca ttttttttga acattgtata atagggccca
     1741 cactcagatt aaaaagcaca aaaaaatgaa ttatcagaac gcaaaaacat ggccctttgt
     1801 tgaagcaaaa aaagttttag agcgccttgg tgggaaaaaa ccggaaaagg gctatgtttt
     1861 gttcgagaca ggctatgggc cttcgggatt gccccatatt ggcacttttg gcgaagtgtt
     1921 gcgcacatcc atggtgcgtc atgctttttc aaaattatcg gatattccct caaggctttt
     1981 tgctttttcc gatgatatgg atggttttcg aaaagtccct gataatgttc caaatcaaga
     2041 aatgttgaaa gaacacttag gcaagcctct gacaaaggtt cctgatcctt atggatgttg
     2101 tgaaagtttt ggtcaccata ataacgccat gctgcgtcgg tttttagatt cctttggctt
     2161 tgagtatgaa tttcaaagtt caacggattg gtatacaagc ggtcgctttg atgaaacgct
     2221 caaaaaagta ctcgtgcact atgatgcgat tatggatgtg atgatgccaa gccttcgtgc
     2281 ggaacggcaa gcaacctata gtccgtttct ccctatttgt cctgagacgg gtattgtgct
     2341 tcaagtgcac attattaagc gcgatagtgc tgcgggtaca gtgacgtatc ccgatcctca
     2401 tagcggtaaa tatattgaag tgcctgtgac ggggggtaat tgtaagcttc agtggaaagt
     2461 ggattgggca ctgcgttggg ctgcttttga tgtggattat gagatgagtg gaaaggatct
     2521 tattgattct gtcaaacttt caacgcaaat ttgccgcatt ttaggcggga cgccacctgc
     2581 aagtcttacc tatgagcttt ttcttgatga gcaggggcaa aaaatttcta agtccaaggg
     2641 caatggtctg acgattgatg agtggctcgc ttatgcgccc caggaaagcc tttcgtacta
     2701 tatgtttcaa tcgccccaca aggctaaacg tttgtacttt gatgtaattc ctaaaaatgt
     2761 ggatgaatat ctttcgcagt tatcaaaata tgatacgcag ggggaggcgg aaaaattaga
     2821 caatcccttt tatcatattc atcagggcaa gcgtccgaat gcgattcatt cgcccattag
     2881 ttttaatatt ttgctcaacc ttgcatctgt cgtaaactca gaggatcccg ctattttgtg
     2941 gggatttgtc agcaagcacg cgaaggaagc ctcagcagag acgcacccct atttagatca
     3001 actgattcac cacgcggtga agtattatca tgattttatt gcgcctcata agcactataa
     3061 attacccacg gcagttgatc aactcgctct tcatgacctt gttgtggagc ttgaaaaaac
     3121 atctcctgat gcggaggagc aagcgcttca aaccattgtg ttcgaggtcg gcaaacgcca
     3181 tgattatcca gagcttcgcg cgtggtttgg cacgctatat gaggtgcttt tagggcaaaa
     3241 agaggggccg cgctttgggt cttttatcgc tatttatggg gttcatgaaa caatagaatt
     3301 gataaaaagt aaaatataat ataaaaatag cattattaat cacttaaaaa taatgcatta
     3361 aaatgaaaaa agtttttcta ctcacatcgc tgatgtttgc tgtgtccata gagtctggga
     3421 tttgctctgg gttcggaaag gatatttatg ttaatttaac aaacagcaat aatacttgct
     3481 taaatccctc ttatgtatca gtaaataata atttgaatct tgataaattt aatgaaatta
     3541 aaaatctgga taaagacctt aaaaatacag atctcaacaa attaaaatac gaaagcaatc
     3601 tttataattt caataataaa gagctgagcg ggaacattaa ggaggtttat aaaaactctt
     3661 acgaagaaaa taaaaatgaa ctcccgatga cggtggaggg aaaagaagac caaaaagaca
     3721 ttaaaaaaac cctaacaggc cttgataaaa agcatacacc cgatccatat gcgttatttg
     3781 gagaaaattt cgaaaaagtc atgaaacttc tgataaacga agaagctaat gtgaaaatcc
     3841 cctctgatcc taacagggat caaggatata atatgaacct tctcacaccc tttcactgga
     3901 agacgctgga cattatcaac aaaagcagga acgatatgat cgaggttggt cctggtgcta
     3961 ggggaatgac acctagaatt cttttgctta aaaataaaat taaaataacg gcgattgagt
     4021 ttgagaagaa agcggcggag gaacttcaaa aaactatcca aaaatacagt gaaacaacgg
     4081 gaattaaggt gtcggggcgt tataatatta ttaatggcga tgcgattatt caacttccaa
     4141 gccttctaaa gcaggagaaa tacaaaaagc ccctttcctt tgtggctttt aatgttattc
     4201 acttgctcac gccaaagcag tttgacactt tcatgaaaac gatctctgat catattaaaa
     4261 tctatggtgg tgatgtgttt atcacggcgc aagctcctgc tgggaacaac ggtgttatta
     4321 agcagtacct taaaaatatg aaaaataaca tcaaaaaccc ggggcatatt cttacgacaa
     4381 aaaaatatac aatggttctt gagccccatt atgatggggg atttgctgag aacatgaagc
     4441 ttagaaagga aacagggaca gtttattcaa caccgaaaga tatcgacaaa tattctccca
     4501 ccgaatggaa aagcacacat gatgaatcct ctgcaaagat tgtcggccat aatcaaattc
     4561 aaatgaatat ggagtcttca aaatcctttc attactttac aacgggtgat ttgatttcta
     4621 aaatagagga aaatggcttg agtccagata atatcgaaac cataggctat ttagatgccg
     4681 gtagaaaatt ttctgataaa ctcgatttca acaatgaaaa tacaaaaaag accattcact
     4741 ccgtgtatct ccatcttaaa aaatagagat taattgatgt ggcacgtgca tttgtggtac
     4801 tttgactgtg aaaaaataac acataaagga attgagcgat gggcatgagc atggggcatt
     4861 taattttact cttagtgatc gtgcttgtta tttttggtgc ggggaaaatt cctcagatta
     4921 tgggggatgt tgctaaagga ctcaaagctt ttaaagaagg gatgagggag ggggataaaa
     4981 cgcctgatcc ttcgtccact ccgcgcgacg aaaaagcacc gataaagacg acgattattg
     5041 accaccatga ttgattttgc ctggtcagaa ttttttgttc tcgcaattat cgcgcttatt
     5101 tttttagggc caaaggagct tatccttctt tttaaaacac ttggaaaatg ggcggcgaaa
     5161 atcaaagcgc ttcagtccct tctccacgag cacattaacg cagcatcggt tgagatcaat
     5221 gatgaaaaga caaacgactc ccgttgagaa ttctactatg acagagctaa gcgttgttga
     5281 tgatcatttt gaggtgtgtg aggaacccct tgatacgccc cagcccctca ttgctcattt
     5341 gattgaactt aggcagcgtt tgatgtattg ccttggaatt ttcgccgttg cttttggggt
     5401 gagttattat ttctcgcaaa cgctttttga atttttggtg gcacccctta cacaattact
     5461 ccaaggacag gatcgcaaac tcatttatac gggcctgaca gaagcttttc tcacttatct
     5521 taaagtgtca ttattttcgg ggttttttat cacatttccc cttatcgccg cgcaactttg
     5581 gcgatttatt gcgcctggac tttacctaaa ggaaaaaaaa gtctttcttc cttttatcat
     5641 cgcaacgccc ttgctttttt ttatcggagc agcctttgcc tattacgttg tgtttccctc
     5701 cgcctatagt tttttcctca gttttgaatc ccctgcgacg gacacgatgc ttgcgattca
     5761 gctcgaggca aaagttaatg agtatcttag ttttgtcatg cgcctcattc ttgcgtttgg
     5821 tgtgtgcttt gaacttccta ttgttctagc acttcttggc cgtgcgggat ttttaactgc
     5881 gcgcagtctc atcgacaaat ggcgcttggc cgtggtgggg attttcacgc ttgcggcgat
     5941 catcacgccg cccgatcttt ttagtatgct ggcgctggca ctgcctctca tttttcttta
     6001 cggtgttgcg attgtccttg tacgctctct tgaaagacgt gattcctaaa attattttaa
     6061 tcattcatat tattttaatt ttttcactgt atattgacgt atgtgcataa atattatgca
     6121 tatgtgatca tcttaaaaat ggagtaacct tatgacaagc aacgagcaac gagcaacgag
     6181 caacgagcaa caagtttaga ttttttcttt taatcacggc attctccctt ctttgtgcgc
     6241 cacttgccca ggcgtcagat tcggatgata atgaaaaaga agaacggctg acacgtgtga
     6301 taaaggccgt taagaaaatg aacaataaac aattagaaaa agtagaaaag cttattaatg
     6361 atctcatgcc gccaaagctt gagcctcaag atgataaaga tgagctttgt tctcagtata
     6421 caaagttgat gaacagtagc gtgtattccg ttgaggatgc aaagacatgg gctaaaaaag
     6481 ccagaaagct ggatgccttt aaagcatttg gccaaagagt taggttggat ggcctgccca
     6541 gaaataaaga acaaaaagaa aaatacaaaa ctattttggg attttagttt ctaaaactct
     6601 aggagagagg cagggaccta gagtctctgc cccttaaatc cttcataaag ggcaatggct
     6661 gccgcatttg agacattcag tgtggtaaac tcaggcgacg tctcaaggtg tgcgaggaga
     6721 tcacaatgtt tttgtgtcag atcgcgcatt ccgtcgccct ctgcgcccat gataatggcc
     6781 attttcccct tccaatttat gcttgaaagc gtttttttcc ctgattccgc aaggcccacg
     6841 gtccaaaacc cttttttctt aagcgttgtc agcgcttggg ctaaatttgt cacgcgcatc
     6901 attgcaacat gctcaagtgc gccacaggct gttttggcaa gcacacccga ttcattgggc
     6961 gcatgccggt ccgttaaaat aagggctttg acgccaaaaa cagcacaagt gcgcaaaata
     7021 gcccccacat tatgaggatc tgacacttga tccaaaatca taacaagctg attttcagaa
     7081 aaatcctcaa gattttcaag gcttaggctt ggcaaagggg aaacgcttgc ggcaatgcct
     7141 tggtgaaccg cgtcactccc taaaaggcgt gcaagagatg gtttgtctgt aagggttgct
     7201 ttttttttaa ggttaaaaag agaaagatcc gtcgtttgtg tgtagagaat ttcatgaacc
     7261 gtgcggtggg gatttacaag ggcatgttgt acggcatgtg tgccgtaaat ccaataacga
     7321 gaacgatcga gtgacataaa atgcggacct taggaggaaa gttgagagaa ttatagcttt
     7381 ttatatccta tttttatggg tttgccggga ttttttttcg agtgctttgt ctgaggggcg
     7441 cgttaaaaaa ggcattgtcg gatcgaaaaa aggctgcttg agggattttt tctcgtttta
     7501 cggagatttt tgcgtgttaa aaaaggctgc ttgagggatt tttgcgtgtt ttagtgattt
     7561 ttgggtgttt ttagggcatt ttctcccatt aaaaggcgat ttttggggcg ctgtgtgaaa
     7621 aaatcaggga tgagggcgag aatttccgtt aaaaaagcct gcttgaggcg atatttcgcc
     7681 tttatagaac tttt
//
LOCUS       DAIAHG010000045         7565 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_45_length_7565_cov_2.739049, whole genome shotgun sequence.
ACCESSION   DAIAHG010000045 DAIAHG010000000
VERSION     DAIAHG010000045.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7565)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45_length_7565_cov_2.739049"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2068 a         1461 c         1841 g         2195 t
ORIGIN      
        1 gaaattattt cgtgtcattt cgtttttttc gtgaaaaatg caaagcagtg tcttcatttt
       61 gcattttttc ttttttttgt gtaaagtggt gagcagtttc aactttttct atattattca
      121 aattaatgtc ttctattacg gttgcggccc tttataaatt tatcagcctt cctgactaca
      181 aagatttgcg agcccctctt ttgagcaatt gcaagcgtct tgggatcaag gggagtcttc
      241 ttcttgcgga ggagggaatc aatggaacca ttgcggggac gaaagaggcc attgaggaaa
      301 ttcatcacta tttgatgcag gatgagcggt ttcatggcct tgaatacaaa gaatcctttg
      361 cagagaaaat gcctttttat cgcatgaagg tgttgttgaa aaaagaaatt gtgacgctgg
      421 gtgtgccggg gattagccct ttaaaaaaag tgggaaccta tgtgaatcca caaaagtgga
      481 atgaactgat ccaagatcct gatgttcttg tgatcgatac acgtaatgat tatgagtgtg
      541 aaattgggat gttcaaggga tccatcgatc ctaaaacaac gatttttcgt gagtttacag
      601 agtatgtgca aaaaaatctg gaccctcaaa aacacaaacg tgttgctatg tgctgtacgg
      661 gcggtattcg ttgtgaaaaa gcatcctctt atatgctaga gcaaggcttt gaagaggtct
      721 atcatctgaa gggagggatt ctttcttacc ttgaaaatgt tccaaaagaa caaagtcttt
      781 gggagggggc ctgctttgtt tttgatcata gggttgctgt aactcacgat ttagagctga
      841 cggactatca aacatgttat ggctgtcgtt ggcctctctc tgcagaggac cgtgcatctg
      901 ttcattataa aaagggtgtg cattgcccta aatgttatga cacattgagt gatgcaaaaa
      961 tgaaagccgc agcacaccgt caaatgcagg ttgagcttgc gcaaaagcgt gggacgcatc
     1021 atattgggga gcgctcataa aaaatagaat atcatctgtt ttaatctaga aaaggatctg
     1081 gaacaaaaag cctcaccccg ttgtctctgc gtatagtgag tgagtataag cctcttattt
     1141 tcccagtgag gatcattcaa ttgtgtccaa agatggaggt aaaaacgcgc tgactctccc
     1201 cacgttctct cggccataaa aatagctcaa gtcttgggca gatcatgagt caaattctaa
     1261 tcattaaccc aagtaggata aatcctgtcg agggcattgc ttgctaaagc ttcgcggaca
     1321 gcctccataa gacgattcat gaaaaacaca ttatgcgccg tgataagtgt atgagcaaga
     1381 agctcttgtg ctttgagcaa atgatgaaga taagcctttg agaagtgggt gcacgtgctg
     1441 caggcgcagt ctggttcaat gggggtgtga tcatttttgc accaaccatt atttaaatta
     1501 atgtgatcgc gccctttgac cgtattattc tctgttttca caagtgcacc gccgtggcgg
     1561 gcgaggcggg tcgggtggac gcagtcaaac gtatcaatcc cctgtgcgac acctttgaaa
     1621 atatctttga cacccccaat gccaagaagg tgaatggggc ggcttggatc aagttctttg
     1681 gctgtcatct ccacaacgtc atacatttgt gctttatctg cgccaagaga ccctccgatg
     1741 gcatgaccga aaaaagcatg ggtgttcaca aaatcacagg attccttgcg aagatcctcg
     1801 tacacacccc cttgaataat accataaaga cgttgacttc cttgcgtatt aagatcatta
     1861 aaagccgtga gggagcgcat cccccagcgg tgactcatac gcatggagcg ggccgtgtaa
     1921 tttttatcca catgaaaagg cgtgcactca tcaaaactta cgatcaaatc agcgcccaat
     1981 ttgacttgtg tttcaatgga tttttcaggg gtcaaaaggt gaattttccc atccatatag
     2041 gagcgaaatg tggctccttc ttcggtaatc tttaaaagcg ttttcgggcg aaggtttgtg
     2101 cgttttcctt tgacctctga gctgacagaa ccatggccaa ggctaaagat ctgaaagcca
     2161 ccagaatccg taagcatggg cccattccat cccataaatt tatgaagacc accggctttt
     2221 tcaacaatgt ctgcccctgg ctgaagaaaa agatgatagg tgttggagag aataatttgc
     2281 gtgtgcgctt ctttcatcat cgagggcgtg acgcccttaa ttgatgcttt tgtggcacag
     2341 aaaataaacg ctggcgtttt gatcacaccg tggggtgtat gaatttctcc aaggcgtgcg
     2401 cgcccgtgcc cagagggggt ataatgaaag tgaaaggatg tcatgaatgt ctaataccta
     2461 tagcctttgg taaaattatc accgtagctt ttggggggag gtgcattgaa aggctctgta
     2521 ataatgtggg gggtaagatt cttttttttg cagtaaatca tcgcttcctc aagggtatca
     2581 aagcgtatat ctacctcata ggctgtgtct gtcgtgcctg tccagcccat aacgttatca
     2641 ctaaaaagag actcagggtg ggagcttttt aaaacccagc catgtgtttt tgcttttcct
     2701 gattgcattg ctgttttgga gggttgatag agacgcacat cttgcataaa aaatctccat
     2761 aggatttggt cggggcgata ggatttgaac ctacgacatc ctgctcccaa agcaggcgcg
     2821 ctaccaggct gcgctacgcc ccgatgactt tgtttatata ggaaatagga taacttgcca
     2881 agtgttaagt ttatcttaaa agcatgcttc ttgtcgtttt ttagcgtttt tcatggttta
     2941 ttaacttttt aacgtttaga ataaaaatac gaacaaagta gagataatca aaaataaaga
     3001 gaaggtttta cccatgaaaa aaataataac ttcatttgca gtgctgctta tgacgcaagg
     3061 tgtctacgca agcggtgatg ttcccctttt ttccaactat aagggaaaaa taacactgaa
     3121 gaagaaagaa gaaaatcaaa agaatgagga ggatgaaaaa gatcttaatc tgaacaaaaa
     3181 taaaaatatc attgatttaa agaataaaaa agaagacaac gagaaagata aagagaaaaa
     3241 agagattgag cgaaagaaaa agatttttaa gttttttgag gggactgaag tcatccccaa
     3301 tgacaaggtg gtagagcttt ataacgtcgt cacaggcagt gatgtgaagg gtattgatga
     3361 tgtcaatttt gtgaagcttt ttgaggaggc tgcgaaccaa tcggttaaaa atcctgagta
     3421 ctctgttgtg ataaatttat ttggagggga taacaataat cctcgttata aggagaaaaa
     3481 gcaaaggctt ttgaataaaa atgtgggtga tgcactcaaa cagatttacg aaataaaaaa
     3541 tctaaacaaa gaattcaaag aaaaagtagg aaaaattctt ggtaaaaaat aaaaatcccc
     3601 atcccctgag atttttctga ggggatgagc cttctttgat gactattttt tggcttctgt
     3661 ggcaggtgcc gcttctgctt ttttctctgg agaagatgca gttgactcag gcgctgttgc
     3721 ctctgttgat gcggagtccg cagttgtttg agatttagcc atctcttcgg cggctgcttt
     3781 tgcctttgcc tcttcagcct tttcatccat aggcaagccc gtcttgtaat caatcagctc
     3841 tgactttgta tctttaagaa catcggtcaa tacctcggca aagaactgtg gcatcacaac
     3901 ttttttaagg tcaggcgcta cttcctcgaa ggttggatcg gggacatcgc gctgatccat
     3961 ttttttgaac acagcccatt tatccttgcc aagctcaacg gcagaatcaa tgacaacgcc
     4021 cgcagccgct ttttggattt tttcagcaag atccttgggg agttcgctaa agcgcacata
     4081 gccaagctct ccttttgttt ctttaacttt atcatctgtg gaaagggcaa caagatcttc
     4141 gaatttttca ccttttttgg cgcgtgcaag cagggcttcg gctgcggctt tgtccttgac
     4201 aatggtgacc atcaagcgga attctttttc ggattttccg tcttttttat agattttcat
     4261 gaattcatca aattgctttt tgagaaactc aggtgtgatt tttgcattga gtttttcatc
     4321 aacgaaatat ttaactttga cagcttccga ggcttcttgc attttctttt gaacctcggg
     4381 aatcttatca acgcccgctt tattggcggc atccacaagg gcgcgcatat cgcgaaggcg
     4441 tttgaccatg gatatataaa ctttcgaaag ggggagagcc ctcacttgat cgggggctgc
     4501 tttaagcgca tcaatcagct gagcaacagt gatttcatca ccttcaacgc gtgcaacaac
     4561 ttgctttgga tcaagcttcg cgagggtttt aagagcaggc tcatccgcgt cttggtcaag
     4621 tttaaaattt tgaccagatg cttttggcgc ttcattctta gcatcaactt tagcttctgc
     4681 aggtttagct tctacagatt ttttttcatc tgttttaggt gcttcagcct ttgcttttgt
     4741 ggtggattct ttttgagtgt ctgtggattt cttttctttc tcctcattgc aacctgcaag
     4801 tgagaagggg agagctacgg ttaaaaagag agctaaaatg ggcgatgatg ataaaaaggc
     4861 tcgcatctgt cgatttcctt tgttaaaaat attaatacca ttgagtcttt tctaggacac
     4921 aattaagata agggcaagag ggaaaaagca cctcaagcga aatagttttc tattttgata
     4981 catagaagca acaaaaattc aaaggacgtt atttaatgtt atctcatctt ctaaagacca
     5041 tcttcggatc cgctaatgat cggtttctta aaaaaacgaa aaaaacagta gagcagatta
     5101 aggaccttga gcctgtgctt gagaagctat ccgatgaaga gctgcaagga aaaaccgctg
     5161 agtttaagga gcgtttgaag gcaggcgaaa cgctggacga tcttcttgtc gaggcctttg
     5221 ctgttgtgcg tgaggcgggt aagcgcgtgt atggcatgcg tctttttgat gtgcagatgg
     5281 tgggtggtat tgtcttgcac aagggcatga tttctgaaat gaaaaccggt gagggtaaaa
     5341 cattcgtggc aacgctggcc gtgtatttga atgctttatc aggcgaaggc gttcaccttg
     5401 tcaccgttaa tgactatttg gcttcgcgtg actctgcctg ggcagggaaa ctctatcatt
     5461 ttcttggcct caccgtagga tgtgtcgtgc atggtattag tgatcgtcaa cgtcgtgacg
     5521 cttatgctgc agacgtgacg tatggaacaa accacgaatt tggttttgac tatttgcgcg
     5581 ataatatgaa gtttcgcctt tctgagatgg tgcagcgccc ttttaacttc gcgattgtgg
     5641 acgaggcaga cagtatcctt attgatgaag cacgtacccc tcttattatc tctggctctg
     5701 cagaagactc ctccgatctt tatgaaaaag tggatgtgct gattcgcaat ttggttgaag
     5761 gggactttga aaaagacgaa aagcaacgca cagtgatgtt gacggatgcg ggtatggagc
     5821 gcatggaaaa tctttttaga gattctggta tgctgcgtgg tgaaaccctt tatgatgtgc
     5881 aaaatattag tcttgtccac catatgaact cagcattgcg tgctcaccat atgtttgcgc
     5941 gggatgtgga ttatatcgtc aaagatggcg aggttgtgat cattgatgag ttcacagggc
     6001 gcatgatgga tgggcgtcgt tattctgatg gattgcacca agcgcttgag gcaaaagaag
     6061 gtgttgatgt tgaaatggaa aatcaaacac tggcgtcgat tacctatcaa aattattttc
     6121 gtctctataa aaaactaggt tgtatgacgg gtacggccat gacagaagct gcagagtttg
     6181 aggagattta taaactccac gttgcagaaa ttccaacgaa tgttcctgtg attcgtatgg
     6241 atatggagga tgatgtttat ctgacagcgc gtgaaaaata tgctgctgtg attgaactga
     6301 ttaaagagcg tcatgccaaa catcagccac tgcttgtcgg taccattagc attgaaaagt
     6361 ccgaggtctt gtctgagctt ttgaaaaaag agaaaattcc ccatcaggtt ttgaatgcac
     6421 gccaacacga gcaagagtct ttgattatct ctcaagctgg ttgtccgggg gctgtaacca
     6481 ttgcaacgaa catggcgggg cgtggtacag atattaaact gggtggaaac ctcgatatgc
     6541 aactagagca tgcgctttca cgcattgagg atgagcaatt gcgcgctgag cgtgagcgtg
     6601 aaattcgtgc acagcatgcg cttgatgagg aaacagtgaa aaaagcgggt gggctttttg
     6661 tgattggtac agagcgtcat gaaagccgcc gtattgataa ccagctgcgc ggtcgttccg
     6721 ggcgccaagg tgatcccggt ggatcaaaat tttatgtgtc tctcgaggac gatctcatgc
     6781 gcatttttgg ctccgagcgt cttgattcga tgctccgccg tttaggtgta aaagaagggg
     6841 aagccatttc tcatggctgg attagcaagg cgcttgagcg tgcgcagcaa aaagttgagg
     6901 cgcgtaactt tgatattcgc aagcacttgc tccgctatga tgatgtgatg agtgatcagc
     6961 gcaaaattgt gtatcaacag cgtcttgaaa ttatgaaagc ggatgatatc tccgagatgg
     7021 ttgatgccat gcgtcatgag cttgtggagg atcttgttaa tgatcatatt cctgcagata
     7081 cattggcaga gcaatgggat gttagcggcc ttgaggcaat agtagagcgg atctttagcc
     7141 tgacgctcga tattaaaaaa tgggtggaag ttgatggcgt ttctgaagga gaaatccgtg
     7201 agcgtctctt ggatgctgtg gacaaagcgt ataaggcaaa agaggtggag agtggcacag
     7261 agcaccttcg cgatgctgaa aaatccgttg tgttgcgtac tcttgatgct gtgtggaaag
     7321 aacatctgct tgtgcttgat cacttgcgcc agggaattaa cttgcgtgcg tatgcgcaaa
     7381 ataatccgct taatgagtat aaacgtgagg cctttaatct tttccatacg atgcttcgtc
     7441 atttgcgtga ggacgtcaca ggtattttga tgcgttttga tatgtcgcac acatcgaaaa
     7501 aatccttgga agatctcctt ccagatatgg atttgtctga tcttgaagag cttctcccag
     7561 attgg
//
LOCUS       DAIAHG010000046         7191 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_46_length_7191_cov_2.694968, whole genome shotgun sequence.
ACCESSION   DAIAHG010000046 DAIAHG010000000
VERSION     DAIAHG010000046.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46_length_7191_cov_2.694968"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2056 a         1575 c         1350 g         2210 t
ORIGIN      
        1 tcaccaaacg cataaggcca gaaatcactg tcgagctggc ttacggcata gtcaatcacc
       61 ttgagggctt tatccctatc gctgatgctc tccttcaaaa tgtccccaat gggcgtgagc
      121 tgattctcga tcttgttctc gtcttttatt tcgataatat tctcttcttt tttctcgatc
      181 tttatttcct cgtctttttt ctcatcattg atattgagat tctcttcttt tttctcgatt
      241 acattctcga tcttctcttc ttttatttct tcgtccttct tgattatatc ctctttcttt
      301 ttcttctctt cttcctcttt ctcatttttt atttcgataa tctcttcctc gttcttatta
      361 ttctcgattt tctcgagctt ttctttcagc ttcttctctt cttcctcttt ctcgagcttt
      421 tctttcagct tcttctcttc ttcctctttc tcgagctttt ctttcagctt cttctcttct
      481 tcctctttct tgagctcgtc tttcagcttc ttctcaacat taatattatt acgatgatta
      541 ttgataagat tgaagtaata ctcttgtgca tcactaaacc actttataat tgcatcattg
      601 ccagctgcat tggttaagct atttttcaaa tcttccgtta acccatccca attcttcatt
      661 tggtttctac caatcttagc attattgttt ataaaaccct caggaacgag ttttttttct
      721 tcttccgtaa gcttaagttc tttccacctt tgtgtatatg taagctgatt ttgctgattt
      781 tgctgatcat cctgatcatt catcgcatga actgtcgaag ggtttccacc aagacctgtt
      841 gtcacactaa gtactaaagc cacttttttt aaatgttttt tcatagaatt tttcccatat
      901 ttttttaaat taaccttttt agaattagca ccttcgccat ccaccgacaa gggcttattt
      961 tctttaccct catcctgacc aagagatttt ttgagggcgg ggatcatccc tgggataaaa
     1021 atatcatttt ttgtatattt acaatatttt tctatgtata ttttttgaaa tttttctttc
     1081 tttatcaggt aattcgtaat attgcccaat aaatcattat tcttaatatc gcccaataca
     1141 ttcttatttg acgcgttaag gctgctcaaa ggcataaaag ccgcaagaag aagccaagtg
     1201 atatattttt tagacattga gtgtaccttt ctttttttat tatttctaag aggtgctttt
     1261 ttaagagtat gataaaaaaa attttaataa aattaattta cgtaatctta taattaaatg
     1321 tagagttaat attactcatt ttttatgatt tgactagtct taaaaagaaa gcttgcatga
     1381 atttttaaaa atcattagga tcattctatc gtgcctgctt ggtggaatcg gtagacacaa
     1441 cagacttaaa atctgtcgct taaccagcgt gccggttcga gtccggcagc aggtaccatg
     1501 cacggtaata tataatgact gatcaaaaaa ctctttttca cgaaccccca aaggccaaag
     1561 ttttagcagc gcccccgtcg aaggcgtctt tcacaccgcc aacccttgaa aatggcctgg
     1621 atcctcaaaa agttcaagat catgccatgc tatggctcag gtccggtgcg aaaaaacata
     1681 aaaacagtgc taaggatttt ctgcttcaac aactgtgcga agtgcgcttt aagcaaacag
     1741 aatatgatca aaaaagcctt gttcgccaat ccattctggg atcatagcaa tacttttagt
     1801 tgctgatttt tccttcaaag atattaagaa aattcctctc acccaaaatt tttataaaaa
     1861 aaataaaaaa aaatcataga tattaagaaa attttaacat ttacctgttg caatggggat
     1921 agaaggaaaa tgattcaacg agtccgccca tggtcgaaag tttacaacac aagctcgatc
     1981 gcgtacgccc cccgcgcgtt caaattacct atgacgtaga aataggcaac gccattgtga
     2041 tgaaggagct gccttttgtc atgggggtta tggcggactt gtcaggaaaa cccgcagacg
     2101 ctttgccaaa ggtaaagttt cgcaaatttg ttgaaatcga ccgtgacagc tttaatgaag
     2161 tcatggcgtc tatccgacca cgtctcgtcc cgcgggttgc taataaagtc aatcctgccg
     2221 aagaacatat gagcgcagag ttattttttg agaatatctc ggattttgat cctctttcgg
     2281 ttattaaaca aatagaaccc cttaataaaa tttatcaagc gcgtatacgt ctcaaagatc
     2341 taatgacaaa gctggatggc aacgatgctt tggatgagat gctcaaagaa attattgtaa
     2401 atcccgataa gcgcacagcc cttaaagctg agcttggcat tgaagatgca ccagctggag
     2461 gtgatacacc gcctgcagca cccgccgcat cgtaggagta gaggattatg gctgatacac
     2521 cccttttaga tgaaatgatg tccacgggca aactagcgcg tgatgaaacg cagcaagcgt
     2581 acgccaaaga acttgtcaca gagtttttaa aagaacttat tgaacaaggt gataacgctc
     2641 ccaaagacac tgtagccttt atttcccagc gcatgcagca tcttgatgat ctcatgacag
     2701 cacagctcaa tgagtttatg catgaccctg attttcaaaa actcgaggga tcctggcgtg
     2761 gattgtacta tcttgtgatg aatacggaaa caagtacgca tctcaaactc cgccttctca
     2821 atattaccaa acaagagatc ttagacgatc ttgaaaaagc cattgaattt gaccaaagtg
     2881 ctctctttaa gaaaatttat gaagaagagt acggtacttt tggtggacac ccttactcat
     2941 gtcttattgg agattatgag tttacacgtc acccgatgga ccttgaattg ctccaaaaaa
     3001 tatctcaatt agccgcggcc gctcatgccc cttttattac agcagcgcac ccgaaacttt
     3061 ttgacttgga tgtttttgag catttaggcg ttccccgtga ccttgctaaa atttttgaaa
     3121 gcttagaact tattaagtgg acatcctttc gggatatgga ggactcgcgc tatgtggcga
     3181 tgacccttcc acacacgctt attcgtatgc cctatggtgc caatacactc cctgcagaag
     3241 gtgtgaattt tgaggaggat gtcgtcggcg ccgatagctc taaattctgc tggacaaata
     3301 ccgcctatat tcttggcgag cgcatcacaa acgcttttgc tcattatggt tggacagcgg
     3361 ctattcgtgg tgttgagggt ggtggtatcg ttgaaggtct gccgtcttat acttttaaaa
     3421 caacagatgg cgatattgcg cttaaatgcc ctacacaagt tgccattaca gatcgccgtg
     3481 aaaaagaact ttcagatctt ggttttattg ccctttgcca ttgtaaaggc acggattacg
     3541 cagccttctt cggcggtcaa acaactcaaa aaccaaaagt ttataatacc tccgacgcaa
     3601 atgctaatgc gaatctatcc gcacgtcttc cctatatcct tgcagcttca cgcttcgccc
     3661 attatatcaa atcgattatg cgcgataaaa tcggtagctt tatgacaaag gacaacgtcg
     3721 ctaaatatct taatacatgg attgcaaact atgttctgtt aaatgacgat gcctcccaaa
     3781 gtgtcaaagc aagctatcct ctccgtgagg cacgcatcga tgtctatgat attcccggaa
     3841 aaccaggttc ttatacatcc gttgtgtatt tacgcccgca ctttcaacta gaagagctca
     3901 gcgtttccat tcgtttggtc gcaacgctac caccgcctgc aaagtaaacg attaacctaa
     3961 agagagagat aagattatgg cacaaacctt tggccctgaa gacttaggag cacaattcag
     4021 tcgggttgtg cgcctcggca acgttgctct agccgtacct gttttagccc aaaacggcaa
     4081 tagcaccatt agagcccctg aatggatggt tgagatcaaa gatattacaa aatccaatgt
     4141 ggctgacttt gatacctatg cagagctttt tgatttccac tgggaatact cccgccatac
     4201 gctcggtcat cctggaaatg aaatgtttac atccgcagcc attgaacaca cagaagtatc
     4261 tattctcatc cctgctggaa cctatacagc cattttacgc caaaaactta ataatggcac
     4321 cgtcattccc gaaattacga ttatacgcct tggaaacatg accgatctta aaaagcctct
     4381 tcaaaaaacg acctattcaa attgtatttt tacaagagca tcgcaaagct atgactattt
     4441 tgctattgcc tttcgcttta caaaagtcaa agaggagaat ttcgaattca aacaagagga
     4501 tgaaggtgca ggtggaacga atgctgccga aatcgattat gcagcaacaa ccgtgggtgg
     4561 ttaattaata aacgcattca taagatataa actatcatga aaaaatcatt tgctcagcat
     4621 gaacccaaga aacgagaggg ggcccttgcc ccccttttcg aaaggcttgt tgactttgat
     4681 acagacgcct tgcaagaacc ttttgtccaa aaattttatg attctgaaga ggtaaaagac
     4741 tcaattgagc gtgaacttct tcaccttctt gggacaaaag ttaaaattcg aaaagaagat
     4801 tatttagccc ttgctaaaga tcctcttaat tatgcccttc cttttatgta tggtactccc
     4861 gattttagtt attttgaagg aacaaacaca gaacaatggc aacctttttc gaaatatgtt
     4921 gccaaagcaa ttgaggcctt tgagccccgt ttgagcaata ttggtgtacg cgttataggt
     4981 gttgataatc aaaaacaatt tcttgatatc gagatcaatg caatgataaa tttttgtggc
     5041 tttttcgaag aagcgacctt tgcaatcaaa atgaaggcgg cttaagtacc tccttcaaca
     5101 gacattattt tctcaataaa agtgattgat agagcttgat tgtcttttca tgcatataat
     5161 cttttgaaaa gttttgcaaa acatgggcgc gccctttttg agaaattttt gctcgtctcg
     5221 cttcctccat cattaaacaa tcgtttattt tatcggcaag atctttgtca gaccccggct
     5281 taacaagaaa tcctgtgacg ccatctatac aaatttccgg atttgctcca aggtttgtcg
     5341 caattaaaag tcgctgcatt gacagcgctt caaggctcac gcgccctagc gcctccggcg
     5401 tcacagacgt catcaaaaca atagatgcct cttcgtaatg ggggcgcaaa tccggtgtag
     5461 gctcaataaa tcgaacgtgt ttttcaagct gatgcgcacg aataaggtga tcaatttgct
     5521 gaagataatt ttctttgccc acacgcatca aaagcaattt tatctccgga tattgaggtg
     5581 ttaagcgtgc caatgcttta atagcgacat gaatgccctt aagaggggat agacgagagg
     5641 gcaaaaaaag cacaggcgct gaggccgctc ttttactttg ataaaaatct gtattaatgc
     5701 cctcataaat caatacgacg ttcgccaaag gacagtgaaa cgttacatga ttttgaataa
     5761 aatgcgagat agcaatcact ttttcacccc gcgccatgat ggaattataa aattttttaa
     5821 aaaatgaacg ccccttatac gctgcatgat aggttgtcac aaaaggcacg cccgtcattt
     5881 tacacgcaag ccaagcactc cacgctggcg ctctcgagcg agcatggacg atgtcaatgt
     5941 tgtgtttttt tattattttt gcgagccgaa aggcattgag aaaaattgtg aaagggtttt
     6001 tagagtgaag cggtagcgtc aaatgctgaa aatgataatt tttttctaaa aggcttccgc
     6061 cctttgacgc cacatatgtt tttgactgag gaagcgctgc tgccagatat tctgccattt
     6121 caaaagcagc atgctcaaca ccgccttgat gcatcgaagg aagaacctga agaacaatgg
     6181 ggatggattt tattggcatt ctaggctgct ttcttttctc tcagatacgg cgttgctaag
     6241 gcaaggattt ttttcatcag cgtggacaaa gcatagtcat gtatctcatc catctccaca
     6301 aaaataccct cattttcaag agtattatgc ccctcataat aaacacttac aatcagctta
     6361 aaatgcgaaa aaacatgggt catagtccca atacaagaat tttccacagg ttcaagcgtt
     6421 tctttaaaat ctgtgatggg gaactgccac aagcccgcta aaagtccttt tccttggcac
     6481 ttttgcagca gaatgcgtga gtctttttga taaatccaac agttggcata gcgcgtgggc
     6541 attgattttt taggactttt cagggggagt ttataggcca tatctttttt aaaactatca
     6601 cagtgatttt gaattggaca aaggctgcat ttaggatttt tgggtgtaca taccgtgcgc
     6661 cccaaatcca tcagcccctg agcgaaatag cttggactta aacctccaaa agcaagttcc
     6721 tcttcaaact tttcaatata aggaaccatg actttagaga gatcctttag cgtaaattcg
     6781 agggcaaaaa agcgcgcaaa aaccctataa acattgccat ccacgggaat ataaggatgt
     6841 tgatgggcaa ttgcgctcaa ggctgatgct gtataaggac caatccctgg aagtttgagt
     6901 aaaagccctc gatcctttgg aaattcacca ccataatcgt caacaatcac ctttgcagca
     6961 ttgtgaaggt gatgtgctcg tctgtaatag ccaagccctt gccaaaattt tagcacgtca
     7021 tctatcgtcg cctttgccag atcgttaata gaaggaaagc gctgcataaa ttttaaaaaa
     7081 taaggcacaa ccgttgtcac cgttgtttgc tgcagcataa tttcacttac ccaaacagca
     7141 tacgcatgat tattttcacg ccagggaaaa acttcatgat ttttttcgta c
//
LOCUS       DAIAHG010000047         7169 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_47_length_7169_cov_3.027214, whole genome shotgun sequence.
ACCESSION   DAIAHG010000047 DAIAHG010000000
VERSION     DAIAHG010000047.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47_length_7169_cov_3.027214"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2065 a         1414 c         1663 g         2027 t
ORIGIN      
        1 ggtgcttttt tcttggccgc attcttaaag gcacggtttg ttaaaaaaat aacgacaacc
       61 atcaggacaa gtcctgctcc cgctagaatc ataatccata aaaggatgtg agcattttca
      121 aaaagaaatt ggctaatcgc actcataagg gtaatgaccg tgcttgttca aaaatatatt
      181 gcaacgactc atccagatcc cgcacaagcc tgtaccataa aatagacgtt acaaagagat
      241 aaatgcatgc aatccctgca aaggtaatca cccaagtgcg cacatccgga agacccttgg
      301 acagactcaa cgcaatatta tgctcatacg gtgcatttgt gagagatttt cgtccgctcg
      361 tataaagatc actctcgcgc tggtgaataa gatggaaaag gcgttttttg caaaaatcta
      421 tttttcctcc atcatcaagg tctttgtatt ttccctgaaa accaaggctg aggcagagta
      481 aataaacctg tgcgagttca ttgcgggtag ggtccgctgc gtccaaaaga acttcaaggc
      541 gagaaaaaaa gagctcaccc gccacttggg tgtggaaaag ctgtgtttct aaaaggtgtt
      601 tgcgccattg cttttgaccc gaccaatgca gatttaaaaa aacttcatca gccagagctg
      661 ccatgagata ctcagcttct ctaaaattac tttgagcata ttccccaagc tggtaagagg
      721 ccttgagaga ttgttcttca agaaaaagcc tcagtttgcg ttgaatgctg tggcaaagct
      781 ccacactttt ttcaacctta gaggcttcga aaacaggaat gccattttgg ggtgcttcaa
      841 aggcttcttc gtccaaaaat tcctcggttt cactttcatc ttctatcggt gtagtttcat
      901 caacctctga atccaaaggc acagtgccac tttcgctctc acgcaacgct ctttcttttt
      961 gacgcaggat ttcctcgtaa aaatcttgaa aaacagctgt gataaagttt gaccgtagtg
     1021 ccgtcatgca gcattctcaa tcggttgggg tttttttaaa taaaggacaa ccgcaagagg
     1081 gcgttgctga tcatcatcag aggcattaaa gatattaaga ttttcccctg ttcgaatgaa
     1141 ctctgagggt tgttgaagtt taaacacaag catgccgcgt cctgtgagaa aatccgttga
     1201 ttcttcttgg tcaaggagct tacgaggtgc acctataatg cgccgcaaac gcacagattc
     1261 tacggtgcta tcgctagcga tcacggcatc acgcatccat tcaaacagcg tctcttctgt
     1321 catggttgag ggtgcccgca cgccaatata catgatttgc tcggcatagg acgcgtgcat
     1381 tttaagatag aacaagcgct cccgttgatt gaaaggcaaa atcacaacag attgctcaac
     1441 actgtcgagg agattgctaa tactttgaag aatatcgcct aaacatgcat tcagattatc
     1501 gtgttgatac aaaggcataa gataaggaac cgttgtgagg cgcatagtag aaagttcgcc
     1561 aaaaacaagg cataaaagat cataaaaggc tttggggtga agggattgcc cgcgcagtga
     1621 tccctctaaa agaggaagaa ttttaatcat cgggcgcagt tgatttgccg tttcgcttaa
     1681 aaaagtcgtg cccacttgtt tttgccattt ggaggagaga tattgcgctt tttctcgaat
     1741 acgacttgca atcgacgtgg cttgatcatg aaaaaatgta aagggattaa tggtgataag
     1801 agggggttga aaatctgtga gcacaaaggc ttcatcacta tgtatgactt cagcaattgg
     1861 aaaagacaca tagcgcgggg ggtatgtgtc agccacaatg agtgagagtt tgggatgcag
     1921 gcgtgggatt gaaatgacat gatcactcag attctcatcc gcagtaggcc caccctcaac
     1981 ggaatcataa caaggccact cacctgtcgt ttcagattgt ccttcggctg tctcgggcat
     2041 gcataaataa actttgagag gattcttttg agcatcactt ttgtaagggg tgaggtcaat
     2101 ctcaaggctt ttatgagagg actcaagggg gtagtgaatc acggcattat tcggcagcgt
     2161 ggctttgagc tctgcgatgc gcaaaagatt atcggcaagc gttaacttat caaagacaag
     2221 atgtgtgacc ccccatgtgt tgtgatgaaa aagatctgtg tgagtcgcca ggagggaatc
     2281 aaagcgccgc tgcatttgtt ggaaatgctg ggggctgagg aacataccct cgtgccattg
     2341 aatgagcggg ggaatagcgc gcatagctct tataaaaatc tttaaaaatt ttatttaatt
     2401 atgcggagaa aaaagaacga actcaagaga gaaaggaaaa aagagacaag gagagaggaa
     2461 aagtgtctaa aacgtgcgac ctcccctcca taatttttaa ttttatagat ttatttattt
     2521 ttcttctttt tcttttgtgt gttttctttt ttttcttgat tgagattctt cacttctatt
     2581 tttaactctt tgattttcgc gtttattgct tttaaagagt tttgaagttt aattttctct
     2641 cgctctgcct ggcgttgaag gtcctcctgc tgacgtattt tttgagttat ggttgcatcc
     2701 cacgcggcct cgcaattagg gattttatcc ggaaattcct ccttgcattc tttgtataca
     2761 gccgtatctt tgcagctaaa gcgtgtgcaa tctttaaggg caatggcctt tgcagttgtg
     2821 cctgccagtg atgtaactaa gccgcataaa agtattttta gataaagcat tagtgttcct
     2881 catcaattca agagtacttt cttatagtcc tattatgaca gagagaaggt taataattga
     2941 tgaacgatct agaaaatttt atgtttttag gcagcagtca tagcactttt aataagaata
     3001 gtgatgccat tgatgatgtg ctgaatcgca agggcagaga gaatgatgcc aaaaacacgc
     3061 gttaacacat tggttcccgt aacccccaag gctttcatca aatagtcccc caaaagcaaa
     3121 ctaatgtaag tcagaatcag aatgattagg agaataataa tgacaccagc tgtcataaaa
     3181 tttgactgtg cttccgcttg gcgcattaga acgacaagcg aggttaaagc ccctggtccc
     3241 gcgatcatcg gaatggccag aggaaacaca gaaacatcat cgcgtgtgac agactcttcg
     3301 tcttcatcag aggttgtcga taccataccg gagtgggatt ttgcaatgac catatcgatc
     3361 gcggctaaaa gaagaagaaa gcctcccgca atacgaaagg caggctcaga aatgctcatg
     3421 atatctaaaa gtttatcccc taaaaacgca aaaagaagga ggatgatcgt tccaatcaca
     3481 cacgctttaa gagccgtttt cctcttgtta atgaccgtat cattgcgtgt taggcctaca
     3541 aacataggca caacggcaaa ggggtcaata ataactaaaa gagtgacaaa agcttgccaa
     3601 agaaattcat tcattaaacc aagatcatta aaagattcct gattataggc taccaaagat
     3661 gagatggaat agccaatttt ttttagaaaa ggtgcgatat tctttaagga tttttatcga
     3721 aaaataccct tgacattttt tataaaaacg agcataaaac atacattcac ggcgtttgtt
     3781 gtgatccctt ttaagggggt gtagctcagt tggttagagc gcctgcctgt cacgcaggag
     3841 gccgcgggtt cgagtcccgt cactcccgcc actcccgcca ttttcaaaaa aatctcctat
     3901 cgtttgctga aatatcttta gaaaacttaa agatggttct cagagagtgg gcttttgttg
     3961 caagttgtct caaaaaacac tacataagag aggaagtatt ccaaaaaaaa taatcgtgtt
     4021 atgaaaaaac tttacatcaa aacctatggc tgccagatga atgtgtatga ttcagaaaag
     4081 atggcaacac tcctcgcacc ccttggttat ggacttacag agacgcctgg cgaggcggac
     4141 gttgcgattt taaatacctg tcacattcgt gaaaaggcag agcaaaaagt tttttcggat
     4201 cttgggcgcc tcaatatggt taaaaaagag cgtgaagaag gtggaaaaga cacgattatt
     4261 gccgtcggtg gctgtgtggc tcaggccgaa ggagaagaaa ttatcaagca agctccttat
     4321 gtcaatatgg tctttggacc acaaacctat catcgtcttc ctgagatgct tgctcaattg
     4381 ctacgatcaa aaggtcagga cctgcgcatt gtggatacgg attttcctgt tgagagcaaa
     4441 tttgatcatc ttattcaacc agaacattcc aatgtgagtg catttttgac aatccaagag
     4501 ggatgtgata agttttgtac tttttgttgt gtgccctata cccggggtgc tgaaagctct
     4561 cgcccagtgg acgctatttt aaaagaagct gaacatcttg tgcgtgtagg cgcaaaggaa
     4621 ataacgctcc ttgggcaaaa tgtaaatgct tatcatggaa gcatgaacgg caaggagtgg
     4681 ggacttggcc gtcttctgta tgcgcttgct gagatttctg gccttgaacg cattcgctat
     4741 acaacatcac atccgcgcga tatggatgat gcgcttattc tggcgcacaa agaaattcca
     4801 cagcttatgc cttttttaca tctgcctgta cagtcgggaa gtgatcgtat tttagaggcg
     4861 atgaaccgta agcataaggt tgatttttat atagatatta ttaatcgcct gagggacgtg
     4921 cgtcctgata tagctttttc ttctgatttt atcgtaggtt ttcctggaga aaccgaggca
     4981 gatcatgcgc aaaccttaga acttgtggaa aaagtcaatt atgcacaggc ctattccttt
     5041 aaatatagca ttcgtccggg aacacccgcg ggcattcatg aatggcaggt gccagaggaa
     5101 attaaagacc gccgtttgca ggagcttcag actcttcttt ttcaacaaca aaaagctttt
     5161 aacacgtctt ttgtggggca agtgattcct gtgctttttg acagagaagg gcgtcatgaa
     5221 ggtcagctta tggggcgctc gccctttatg cagtccgtgt atgtagcggc atctgagcgt
     5281 catttggggg ccatagcgca tgtaaaaatt gataaagcta ctcaaaatag tatcagtggg
     5341 gcaattgaca ccacagaaag tgtcttgtcg cccgcgaata cttttgcgga ggcaaaaagc
     5401 aaagcagttt aagggaggct gctagtcttc ttggaatttt ttttattttg tttttattgt
     5461 tgatcaagaa aaagttaatc atcaaaattt ttttttaaaa aatgaaagaa atacatattt
     5521 taactatggt taatacttcg ttaaaaaaat aacactggta aaatggaaaa aatcgcctca
     5581 aaatgatctt aggtgcttgt ttaaaaaagt gggaataaaa aactttggaa caacgatcgc
     5641 aatcgattga gtttgaggat aatgatcgct taagagccct tgtcggatca gaggatgaga
     5701 atttaagttt cattgaaagt cgccttggcg ttgatattgc tcttcgtggc aatcgcatta
     5761 ctattaccgg cgatgatccg gaggtaactt ttgcaaaaac gacgctccgc tatctttact
     5821 cgcaactgcc ccgcattaaa gaggtaaaat ctgacgacat agaagaggcc cttcgcattg
     5881 caagcacgat ggtgaatgtg gatgatccaa ttatcaaggg caaagacaag cataatgtta
     5941 gtatctccac aaagcgtaag attgtttttc caaaatcctc gaatcaagca agttatgttg
     6001 aggccatgca aaattgtgag cttgtttttg gtgttggacc cgcgggtacg ggaaaaactt
     6061 atctggctgt ggccatggct gtgagtttgc ttttagcggg taaggttgag cgtattattc
     6121 tatcacgtcc ggctgtggag gctggagaaa agcttggttt tcttccagga gatatgcgtg
     6181 aaaaaatcga tccgtatctt cgtcctcttt atgatgccct tcatgatctt ttgcagggag
     6241 accaggtcat taaacgcatt ggtgcggggg aaattgaaat tgctcctctt gcctttatgc
     6301 gaggacgcac actttcaaac gcttttgtga ttttggacga agcccaaaac acaacgccta
     6361 tgcaaatgaa aatgttactg acacgtcttg ggcccaattc acgtatggtt attacaggcg
     6421 atttgacaca ggtggatctt cctcatggcg ttaaatctgg tctaaaagat gcattgcatg
     6481 tgctgcacgg tgtgagtgaa attggattta ctttttttgg cgaggaggat gtcattcgtc
     6541 atccccttgt ggcgaagatt gttcatgcct atcaaaatca accctaatgg tttaatctgt
     6601 tttttctatg agaattctta ggggagtgag ttttaacaat ggatgatgca ctctatcgct
     6661 tgaatgtggg aattgtcttg aaaaatgctc agggcaaaat ttttgtagga cagcgtatcg
     6721 atgccgctct tgagggaaat attgcgtggc agatgcctca aggaggtatt gatgaaggcg
     6781 aaacacttga ggaagctgtg atgcgcgagc ttttggagga aataggtacg gataaagtgc
     6841 gcattgtccg tgtgacaaag gaatggcttt attatgattt gccggaggat cttcaggccc
     6901 ttcaggtcaa aaactggggg cggatataca agggacaaaa acagcgctgg tttttgatgg
     6961 attttttagg cgaggactcc gacatcaatt tgaatacggc gcacccagag tttttatcca
     7021 gtgcatggat cacaccgcgt gaggcactcg cttgtgcggc gccctttaaa cacgctattt
     7081 accaaaaggt tttcagggaa tttggttttt tttagggatc aaattttctt atagcaaagg
     7141 cgtggtgctg aagacgggaa tcgaacctg
//
LOCUS       DAIAHG010000048         7168 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_48_length_7168_cov_3.114652, whole genome shotgun sequence.
ACCESSION   DAIAHG010000048 DAIAHG010000000
VERSION     DAIAHG010000048.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48_length_7168_cov_3.114652"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1970 a         1377 c         1547 g         2274 t
ORIGIN      
        1 aggaggcgtt ttcaaaagca ctggggacag gatttagagg cggtctgagg cttttagatg
       61 tggagatcgt acgcgataat ctgggtaagc cttcttttca tcttcagcca agggctttgg
      121 aattgttcga taaccttatt cccattggga tggaggggca tgttcatctg agcctttcgg
      181 atgaataccc ttatgcccag gcttttgtga cgttgagcgc cgattagtcc gtttttctat
      241 gaaaggatgc atggaataag atcaaaatca tcctcatcct tatttttctt gaaacgctcc
      301 tttaagccct cgttttgttt agctaagtat ctatttttta ctttttcaat gtagctttct
      361 gaaaattctt cgaggatttt cttacggcga tcaaagtttt ctgtgctggg gttgagaaaa
      421 agattatttt ctacgatgga atcacccttg aggatgttgt caatgtaagt gtttgtttca
      481 ttatcgtcga atgtcctttt aatgggtttg cgcaacatat caattttaat ctctaggatt
      541 tttttaaaca attttttgtg atccttttga taatcttttt ccatgagggc atcaatatcc
      601 tcttttgcaa aaagagaagg cgcgcttaaa aggccgcaac aatatgctaa gaaaagagaa
      661 acaacgagag attttttcat ggaataattc ttgtctgaag gtttaaaccg ccccagttta
      721 tataaaattt tggctgttga caaaccctta cgcagctttt tttcggtgaa tcatcgtgag
      781 aagaaggcca agcgtttcct tgagtttttt acggggaaca acactatcga ccatcccatg
      841 atcgcgcaaa tactcggctt tttgaaaacc ttcggggagt ttttgacgaa tcgtttcctc
      901 aatcacgcgg gcacccgcaa aaccaataac agcaccaggc tcagccagtg taatatcacc
      961 cagcatcgca aaagaagcac ttacgccgcc tgtggtcggg tctgtgagga gcacaatata
     1021 aggcagccct gcttctttga cacgctccac tgcaattgtt gtgcgaggca tctgcatcaa
     1081 ggataaaata ccttcttgca tgcgcgcacc tccagaagaa ggaatgatca aaaggggcga
     1141 gtgggttttc accgcaagag ctcctgccgc aacgataccg ttgcccacag caaggcccat
     1201 ggatcctccc ataaaagcaa aatcaaaagc agcaatcacc gtattgatac cattaatagg
     1261 accaaaagcg actttgatag cgtcatcaag gtttgttttt tgacgatatt ccttcagacg
     1321 atccgtatat tttttaagat cacgaaactt caatggatcg tggagaactt tttcaagggc
     1381 atgaattgta taagaaccat ggtcgaacaa aagctcaaaa cgctccttag cgcttaggcg
     1441 aaaatgatgc ccacagtggt gacacacacg gctattttct gataaatcac ggtggaaaag
     1501 catgtgttcg caccctgggc attttgtcca taaatgatcg ggggtttctt gcttattccc
     1561 cataagcgct tggattttgg ggcgaacaaa gttagaaagc cagttcatgc gctttttcct
     1621 tttgagggcg ttattggttg tttagaggga gccacttctt gttttgaagc cacagatttt
     1681 aaaggggtgt caaacgaaag ctttgaattc gcgcgtaaag ccacagattt aaaggactcc
     1741 tttgtttctt ttgtaagata gtctgctttg aaatcttttt tgatcttttc aaggaactta
     1801 atggctgctg gatggttacc ttgagcgata agaatttctg ccatacctat taatgcgtct
     1861 gctgcgtgag ggccttgctc atcgccacgg taaacacggg caaaagtact ttgggattcc
     1921 ttcaatttgt tttgtttgag aagcacgcgg cctaaataat aaagagcaga gcggctggag
     1981 ggatccgttt ggtgcgttgt aatatagtga ttgcatgtgg tggtaaaggc ctgtgctttt
     2041 tgggggagcg tggccgcgtc agcaagcata acctcattat aaagtttatg cagtctatca
     2101 aaatcggact gagcaggacc cacaggaagg tgccccgtct tttggtgagc atgggccatg
     2161 gctgcggctt cttttgcact atgtgcgtct aaagatttct caagctcttg ctcatttgtc
     2221 gcaccaatct cttgcatcaa tgctttgaca tcgggatctt gtgtgatatg cgcttgagca
     2281 tttgcaggcc cttgaatagg aatagcgccg ggggctgttg aaggtgtttg acgttggact
     2341 ttaagctgac cttcaagctg ttgtacttta ttcgtaaggc ttgcaatttg agcgcgaaga
     2401 gctgtgatat cgtttccatt cgcacttgcc ggcgaagtcg ctgtttcaaa attctcatcc
     2461 gctggctctt cctctgcagc ttcttcttca ataacttcct catacatcac atcttcttcg
     2521 ccttctgcat aaagcgcctg aggaatgata aaacttaaaa aaggtaaaag ataaaaaaag
     2581 cgcacgatca aaacattatc atcagaggtt aaacattgac aatctagcaa aaatgattaa
     2641 aaaacactag gccctaccgt tttgacgcgc tattttctaa attttttgag cgaatacaca
     2701 ctcaagaagg gttttgtttg cttgagaagg tcctgtgatt tttatttgaa agcgtgcatc
     2761 ggtgttttat tttttgtaag atatggacgt gtatatcctt taatctttat cattttaatt
     2821 taatggcttc tctttctctt ctcacatcta accatccttt ggcgaagtct ctcgagaaaa
     2881 actggcgtgc agattatact tttggccctc cagaaaaggc ggacattctt ttggtagcag
     2941 ggggtgatgg catgatgttg cgcgttttgc gcgaatatgc ttctcaaaaa atctatggta
     3001 ttaactgtgg aagtgttgga tttcttatga atacgctcca tagtgaaaat cttaaagata
     3061 tgcttgcgcg gattgaaaaa gctaaggaaa ttgcgctgca ccctcttgtt ctggaagcca
     3121 caactcttga cgggaaacgt catacagcca aggctattaa tgaggtggcg cttttaaggc
     3181 gcggtgctca aggggctaaa attgcaattt ctgtggatgg tgtgatgcgc atgcaagagc
     3241 ttgtgtgtga tggtgtttta gtggccacac cggctggaag cacaggctat aatctttctg
     3301 cccatgggcc tattatcccc ttgggttcga atctcttagc cctcacccct atttgtcctt
     3361 tcaggccacg acgttggcgc ggtgcccttt tgcccgcaca ctcgtccatt tcttttgaga
     3421 ttattgaccc ggatcacaga gttgtaggcg tttctttcga tgatcaggaa attcatggcg
     3481 ttacacatgt taccgtcaaa gaagatagct ctcaaaaact cgccattctt tttgacgaag
     3541 accatcacct tgaagagcgc attattgctg agcaatttct aagctaagag atcagtgatc
     3601 aaaagatctc acgaatctgt ttgttgatat caatgcggtc tttattaaac tcgatgcgat
     3661 ctggtttttt tattttgttg agaatttttt tagggtgagt gccttctctg tcatgtcctt
     3721 ttttggacaa atacaacatg atcacggaac atttagaaaa tgcctcaggg gccttgcaac
     3781 ttttctcaag gagttcttta aattttttca gggcaattcc aagactctca gggccaaatt
     3841 gtgagacatg atgcagctcc atcgattcag cccctccatc aacaagattg tagcccattt
     3901 caagtctttc tttattcgtt cttcctttta tatctttcca atttttgtga atacctgtgg
     3961 gaaaaataca aagatatcct ttggatgaga caatccaatt ataatatttt ccttcaacaa
     4021 atggtttaag cataagaaaa acagaatact gaaaggatag tgcgcttatt tttttatctt
     4081 cattgtaaag tttgctgata tattgctgta tctgatccgt tatatgattt tcaatatcat
     4141 aaaaactgaa atcccctttg atttctttgg atagtttaag gttttcctct aaaatatttt
     4201 tttgagtaga ctcaatctct tttaaatcgt cctctgtaag ttcttgtttt tcttttttct
     4261 ttttgggggg ggatttaact gcaatttttt tagaaaattc tcttatatcc tggcggctaa
     4321 atttaagtat gcggagcaac tctgggtcaa tgtctgcatc tttgtcgcca cttctttcct
     4381 tatcgatata ctctttaaaa ggtcttttct gtcgataaat tttaaagtaa acctcaagct
     4441 cttcttctga tccacctaca ctcttataag actttatatc ctcttccgtt agaatttcag
     4501 cctgcttgaa aatcgtccaa agaatcttaa ttttctcaag gctaacttta tttttctttt
     4561 cctcatacat ataatcgtcc tgaggaggaa ggttcgtaat attttgaaat ggctttctga
     4621 tgttttttga taaagtgatt atttttttct cgttttcttt attttctgat gcaaccaaag
     4681 gatgctgtgc aagagaaata agcagcgtga ggagaaacaa aaaaggttgt aacatacgca
     4741 tataaacatc aaaaatatta gcaataaata tgtggcattt ttaattgaaa attcaatatt
     4801 tatattttaa tattttttta attaatgatt attttagttt tattaataat ttgaaagaaa
     4861 ttgagtgata tgaaagataa aaatcagctt cgccaggaat ttaaaaacgc acgggatgtc
     4921 tttgtggggg ataaggaagc cctggtcttt gaatccgatt ttcaacagtg ttttgagcga
     4981 tttgttcaag atcaacgcct tgaccttaat gaaaaaattg tggcgtgcta tgagcctttt
     5041 ggaagcgagg cgtctccacg gggtataatc cgctggcttt ttgaaaaaaa cattgaggtt
     5101 gctctccctc aaatgaatgg gaagagcctt gttttcagtc gttatacacg cacgatgctg
     5161 cttgtgaaaa atcattttaa tttttttgaa cctgtggaga aaaagcctct tcttccggat
     5221 attgtgattt taccgctttt ggcgtttgat gaaaaaggtc accgtcttgg ttatggtcag
     5281 gggcattatg atcgtgcgct tgaggaaatt ttaaaaatta aacggccgat gctcattggg
     5341 ctatcttatc cttgtcaaaa agcggcgtct cttcctgcag agccccatga tcaaaaactt
     5401 gatgctgtgc tgctgccaaa ggcatcttta ttgttttagt aagagtgaag gttataaatg
     5461 tgtaaaatcg aaaaattgtg cacattttgt gcggggcgtg ctattgctgt ggtaatgatg
     5521 atttaaagta ttttttgatg acaatgcgtt tggaacatgc tcttggctat gtctttaaaa
     5581 ataaacaaaa cttagaaaaa gcgtgtcacg caaaaagcac ccgacatcag aaaaaaaatc
     5641 aagacttcga gcgtttagag tttttaggtg accgtgttct tggccttgcg atcgcggata
     5701 ttttatgtga aaagtatgga aacgagagcg agggagattt ggccaaacgc tttgctgttc
     5761 ttgtctcaaa ggaatcatgt gtaaaaattg ctgaaaaaat aaatcttcat acctatataa
     5821 cggatagaga cggtaaaaaa gtcgcgatta cagcccactc taatatactt tccgatgcga
     5881 tggaggctct tttaggagca atttattgtg atagtgattt tgagacggtg aaaaaaataa
     5941 ttacctcttt gtggcaatct ttactcgagg gagatctaag gccgccacgt gatgcgaaaa
     6001 ctattttgca agagtatgct caaagctttt ataagtgtgt gcctgtttat acccttttag
     6061 aaaccaaagg ccctcatcat gctcctcatt ttatcatgaa ggtttttgtt gaaggtcttg
     6121 gtgaaagtgt gggggaaggt ttgacaaaac gccaagcgga gcaagccgct gctgattatt
     6181 tactaaagga caaacaaatt ttatgactca tgctattgaa acaaagtgcg gtgttgtggc
     6241 gcttattggt gcacccaatg caggaaaatc aaccctgaca aacgccattg tcggtgaaaa
     6301 ggtgtcgatt gtgacgcaca aggttcaaac aacacgtcag cgtgtgacgg ggattgcact
     6361 gcacggtcaa acgcaaatga tccttttaga cacacccggt atttttaaac ctaaagtacg
     6421 tatggagcgt gccatggttg aggctgcatt tgatgccgca aaagatgctg accttcgtct
     6481 ctttatttta gatgtgggag ggcgtttgga tgaggttttt atgcaagaaa cccttgcaat
     6541 gcttcaatct cccttcatta ttgtgctcaa taaaattgat actgttgcgc ctgaaaaact
     6601 tcttaaaatc gctacctttt ttaaggattc aaaggctgaa aaagtattta tgatttccgc
     6661 tcttaaaaat gatggggtac aggatgtgat tcattttatc gcaaatgcca tgcccctatc
     6721 accgtggctt tatccggaag attatattgc ggacataccg gaacggcttc ttgcggcgga
     6781 aattacgcgg gaaaaactta ttctgcaact gcatcaagag cttccttatg aaacgtacgt
     6841 tgagacagag gcttttgagg tttttcagaa tggcgacgtg aaaatttcac aagtgataaa
     6901 cgtcgcaaaa gaaggtcaga aagccattgt tttggggcac aaaggggcgc gcgtcaaggc
     6961 gattcgagag gctgctcaaa aagaattaac cgtggttctt gatcgcaaag tgcatctctt
     7021 tcttcacgtg aaagtcaccg aaaatttgca agaaaaatcc gatttttatc atctcacagg
     7081 tttaaattat aaagcctaag agttcttgta aggttgaatg tgagtgatac tcttaaatca
     7141 tgaaaaacca caaaatatcg gtcaaaaa
//
LOCUS       DAIAHG010000049         7097 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_49_length_7097_cov_3.424786, whole genome shotgun sequence.
ACCESSION   DAIAHG010000049 DAIAHG010000000
VERSION     DAIAHG010000049.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49_length_7097_cov_3.424786"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2147 a         1385 c         1486 g         2079 t
ORIGIN      
        1 gaaatcactt cttgaaggtg ttctttatct gtggcgagaa gggtttgact gtgaagctga
       61 caccgtgctt ggatcagatc ttcatgcatc catttttctg tcataagtag cagaaaattt
      121 gccatggatg tattttgagt ttctttattt gttttttcga tagcaggaag ttgccagtag
      181 gcgacaaaag caagggctgt gtttgcaagg gcggagattc ctgtaaaaat aatcatttca
      241 agatggaaat cacctttgtg aatttcaata aaaacattga tcattaaaag gcagccccaa
      301 aaaaaggaga gcgtcacaag ccataacaaa acgtgcccgc gcattaaaaa atctcttttt
      361 atatcatatc agccgttttt agtttagatc ttaaaaattg atttttgatt aatttttcta
      421 aacgcttata attaaaagtg aaaataaaaa acatatggat ttttttgttg atattgctga
      481 gggcattatt cctttcctgc ttttctctcc tgatgcaagg gagtgagctc ctttgtgctg
      541 ggcaaacaac aacgccctct tctgctaagc cgtcggttaa aaaaaaatct ttaaagaaaa
      601 agcctcagaa aaaaaaacgt caagaaacat cgcttggcaa aaaaacacct ttagtttccc
      661 caccctccaa aggcgttgat cttccgatca cggaaaattt aagcatcctc gaatcagagc
      721 tgcccaaacg tattgatatg atgaataaag tgagtacgcc cattcttaaa agtgttgagg
      781 gggagtcaaa aaacgcttct tcgaatcacc cagaatcttc tgtcacctct gcctcccctc
      841 cttctataat acaagctaat gatgtttcgc ttacaattca ttctaaaaaa ttaccaggaa
      901 tagcccttat tcggatctta gatgcgtact ggctttttct tgatcaaaaa tatcctttta
      961 cccttccgac aaattatcct tacggtattt tttcaatgga ggataaaagt acagagcaga
     1021 atacgattgt taaaataatt cttcgccgtg ggttttggcc gaatattcgt gcagaaaaag
     1081 aaacgtggac attggatttt aatatttctc ctcgcctcag tcgcacaaaa aaagtgatgc
     1141 gttggccgga tagcattgat aaacctatta aaattttgat gccaagttat gtaggtgaaa
     1201 tcccttgggt tcatccagaa atgaaaattc ctatttctat ttttacctca cgtaaacaag
     1261 atggagcttt tacaccccag caaagctatc ctcaactcac gttgatggag tcttatgtgg
     1321 ggcttgcgat tcagcctata tccgattcgc ttgttgttcg aagagatgtt gaggatcttg
     1381 acgtttttga tgctcgcggt attgcgctcg gcaatgagtt gctctcaagc aaagaaaatc
     1441 tcagtatttt agcagacaca cgcagacctt ataaaattga agatgaactc caatctctta
     1501 aaaataaaaa acgaacgcct gcgattatga tgaaaataat tgaattgagt ctcaaacttg
     1561 gcttttttag tgaagcaggg gcgcaattta aggcgcttga tgctctttgt actcaaggag
     1621 tgtgggtgga tcaggcaaag gttgatcttt ttcgccttgt gttgctgaca ctctctccga
     1681 atgatgagag tgcatcacat cctattgatt ttacacatca caattttcag agtcctgaat
     1741 ttcatttttg gtacaatctg tatcgcaaaa accctcaaaa ctatgagcct ttgattaagc
     1801 atattatttc acactaccct ctttttttaa aaaatgaggt cgttgaaatt cttttgtctg
     1861 ttccggatga gtatgaaaat cttcgaagtc ttattggtct tcaagggatt gatagagact
     1921 taaaagataa agcacgtctt aagcttgcgt tgatggagcc gattgatatt cgtgagctaa
     1981 aagatcttgt gaaatattca aaaaatcgtg aaatccaagc aaaggcacag ttgcaaattc
     2041 tttatgagga gcaaaaagaa aaggatttta atccccttaa ggcaattaag atccttgagt
     2101 catatctttt tagttttggg gataaggagt ttgaggctaa agtctttttg gcaaaacttt
     2161 atgttggtca aagagactat acgcaggcga ttcgtcttct caaagatctt agcctggagg
     2221 ataaaaatcg cagagcagag ttgaatgagc atatgcgata cgcttactta caattctttg
     2281 aagatgtttt ccaaaaggaa gaggtaaaac aaaataactc aaaaaataaa aagaatagcg
     2341 aggggttaga gaattttaat ccgcaacctc tcgatattgt tgcgttttat aatgatttta
     2401 atcacctgac gcccgatggc gagaaaggaa aaaaaattct taacactgtg atcgatgcgc
     2461 tgaaaaaact ttcgctctat cgacccgcta ttgagatttt atcccgtcag tttgagaaag
     2521 agaaaaacgg tcctgataaa acatctctca catttcaaat agcagatctt tataagatgt
     2581 tgccatcacc agaagacgtg gagaaaattt taaacaccct tgatgataca acgatgagcg
     2641 aggctgatgt tttaaaaaaa aggtatcttc ttgtggagag ttatcttttg caaaatcaac
     2701 caggaaaagc cttaagcctt gttgaaaatg ataaaagcct tgagggagaa atctctaaag
     2761 agcgcatata ctggcatctc aaggacttta ataaaatttt agaactcgta ccactccttc
     2821 ttcaaagagt aaatgacaaa gaaaagcgag cgtttcttgc gacacgcctt gccattgcca
     2881 atgttttggt tcaaaatcca ccgtatcctt ttgaggatct tcgtatgcgc tatatcaatg
     2941 atgtaaaaaa cacgatgtac gagaaagatt ttcttgtggc aacgacgccg gaaattcgtg
     3001 tcgaagatgg tcttctctat tttcctacct tggatccgct cttgaagccg aatgcctagg
     3061 tcgagtgaat ttgacgtgcc cataattttg tgtaatcgcc accaagtgct aaaagctctt
     3121 tgtgggtgcc acgctcacta atttcaccat ttttaagaat aataatctga tccgcgtgga
     3181 caacagtgga gaggcggtgc gcaataataa tggtggtata atttttggca agcccctcaa
     3241 gctggcgttg aatatgcaat tctgtctgac tatcgagcga cgatgtagcc tcatcaaaaa
     3301 taaaaatacg tggatttttc aacagggccc ttgcaatggc gatgcgttgt ttttcacccc
     3361 cagagagctt aagcccacgc tcgcccacaa tcgtctgata tttttccggc agagattcga
     3421 caaaatcatg aatttgtgct ttttttgcgg cctcgataat atcctttatg gaggcatctg
     3481 cctttccata actaatgttg tagccaatgg tgtcgttaaa aagaaccgta tcttggggca
     3541 caataccaat acatttacgc aagctttttt gggtgagcgt gcgaatatct tggccatcaa
     3601 tttgaatgga tcccgtgctg acatcataaa aacggtaaag gagttttgcg atggttgatt
     3661 ttccagcgcc actcacgccc acaatagcta gcgtttgttt gggcgctaca ttaaaagaaa
     3721 tacctttaag aatgggacgg ttgtttgcgt agtgaaaggt aacgttgttg aaagtgatgg
     3781 caccttgctt taggtgaaaa tcttttgcat aaggggcatc ttgcacatca agatcgacat
     3841 caaggagatt aaacatttgc tccatattca caagggcgag gcgcacctga cggtaagcaa
     3901 accccaccat aaagagggga ttaaaaatct gcagaaggta ggcattaatc gcgacaaaat
     3961 cacccagggt aaactgctgt gtcttgatgc gttgcaaggc aataagaaga atggcaaaaa
     4021 gtcctaaact tgtgacaatg ttttgagcaa tgttaagcac agaaagggaa attttacttt
     4081 tgacagcagc cttttcataa gaagcaagcg ctttgtcata gcgccgtgcc tcaaaatctt
     4141 cattattaaa atacttgact gtttcgtaat taataagact gtcaatggct tttgagtttg
     4201 cttctgcatc acttgtgttc atgtttttaa tatagcggat gcgccattct gtaatatgaa
     4261 gcgtcgaata aacataaacg accatcatgg ctgcgcaaat aaaggcaacc aaaagcccat
     4321 agagataaga aaaaataatc acaataaaga tcacttcaat caatgttggc aaaagagaga
     4381 acgttgcaaa ttgcataaat tcctcaatgg ccttaacccc cttttgaata atttggttca
     4441 gggcccccat ttttcgctca aggtgaaagc tgaggctgag attgtggaga tgtttaaaaa
     4501 ctttgacacc tgcttcgcgc atggcgcgct cggcaacgcg tgcaaaaatg ccatcacgta
     4561 cttcgttcaa aagccctgca atgaggcgtg atcctgtgta ggcgcagaca agggcaaggg
     4621 gtgccataat aatcgcatga ccacttttca acccatcgat caggtattta aaaaaaagag
     4681 gtgttgcaat ggcgaaaact tttccaagga caagcatcgc catcgccaca ataaagcgga
     4741 ggcgtgcgga caaaagtcct ttgggccaaa tatgcggcca aagctgcatg aatcctttga
     4801 gattcagttt gagacttccg gagggaagtg ggtcgtcttt catgcagtga ctgttgaaaa
     4861 agaatctact catagaaatt ctaacaatca aaagtaaaaa aatatataat ttttgatacg
     4921 gcgttttgct ctttatgaat ctcatttccg ctaaaaaata gagggaaagc tcctcctaaa
     4981 gtgtgtggga ggggcactca agcccgacct tccccactac tctttactta ttataaaaaa
     5041 tttttatatc cttataattt tcaaagttct tgaaatcatt ttttacttta ttttcattac
     5101 gtttgtatat ctttttaagt ttttctctct cctcttcatt gagcttaaga ccctcaataa
     5161 atagaatcga attatcgtat ttattaagat cattcaggat ttttcgagag cccttcctct
     5221 taaggtagtt actcccgaga cccaatgtct caagatttgg attattctgg atgattttga
     5281 taagaaaagg aacactcttc gaggtgagct gattcgcaaa taaatctatc cttttaagag
     5341 acgtatcttt gagggtttca ctcaaaatct caatccccgc atccccaagc ttattacccg
     5401 cgagattcaa tgtctctcca ttgtcgtagg tattcatctg aatcagctgt tttattctct
     5461 gagtctcctt atcactcctc tcactctcaa tttcatcatg agtctcctcc tcactcgccc
     5521 tcacaggaga aaggtcaaat gctacccccc catatacatg attgacatta gtgtcaatat
     5581 ctttttcata aaattactcc attttgttaa agttaatgat tgatcatcca tctttataga
     5641 tgacagagaa aagtaaagaa agggttaacc ttgcgctcta tattagaaaa aaagtgtgtg
     5701 gaggaaaatg acacgtttgt catgatttgt cactggtcaa gggatcgtgt ttctggcaca
     5761 aatgtttgag tgaaatgata caaaaaaaga aaaaagtgat gcgtgctgtt attatttttg
     5821 cggctggaca agggacacgt atgcggtcga ctctgccgaa ggttttgcac acaattgcgc
     5881 ataaatccat gctatcttac gtttacgata cggctaaaga tctcgatccc gcacagatta
     5941 ttctcgtggc gtctgagtct ctaaaacagg cgattgagaa aaaagataaa acgattgatt
     6001 ttgatacaag tgagctgact cttgccgttc aagataatcc tctcggtaca gggcatgcac
     6061 tgcaagtggc gtttcattac cttcaccccc agattactga aatcctcgtg ctttatggag
     6121 atgtgccctt tgttaacgtt gaaacgcttc agccgttgct cgaggataat catgagtgtt
     6181 gttttttagg catgcatgta acgcctcccc atgcttacgg tcgcatgatg actgagggtg
     6241 aaaatattct caaaattgtc gaagataaag atgcaacagc ggctgaaaaa acgattaatt
     6301 atgtgtggtc cggtgtgtta accgcaaaaa cagctttttt aaaagactat cttcctaagc
     6361 ttgcgccaag tcctgtgaca ggtgaatatt acattacaga tattattaaa tttgcggcct
     6421 tggatggcaa aaaagtaagg cacaaagaaa caccttgcgc tcttgagttt gaaggcgtta
     6481 atgacaaagt ggctcttgcc cgtatggagc agcgtcaaca agaagcaatg cgtgcggatt
     6541 ttttagaaaa aggtgtgaag atgatcgctc ctgagactgt tttttttgcc cacgatacag
     6601 tgattgaggc ggatgtaaca ctgatgccct ttatcacttt tggtaagaat gtgtgtatta
     6661 aaagcggtgc attgattttt tcgcactgcc acatcgaagc atccgtgatc gaggagggtg
     6721 tgcgcatcgg cccctttgcg catttacgtg aggggaattc tattggcaaa ggggcggtta
     6781 ttggtaattt tgtggaggtt aagcattcga cccttcacag caatgtgaaa gcaaaacacc
     6841 taagttattt aggggataca gagattggcg aggggacaaa cattggtgcg gggacggtcg
     6901 cgtgtaatta tgatggaaaa aataaacata aaacaaccat caaagcacac gtctctattg
     6961 gtgggaatac aacacttgtg gcgcctgtga cgatcgagga taatgttatt gtggccgcag
     7021 ggagtgtgat tacggaggat gtgcccaaaa acgtcatcgc ctttggccgc gcacgccagg
     7081 tgaataaaga gagaaag
//
LOCUS       DAIAHG010000050         6997 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_50_length_6997_cov_3.256214, whole genome shotgun sequence.
ACCESSION   DAIAHG010000050 DAIAHG010000000
VERSION     DAIAHG010000050.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50_length_6997_cov_3.256214"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2022 a         1653 c         1436 g         1886 t
ORIGIN      
        1 cccacacttt taatacgctc tcttttgaag cattcttgcg atcttttgtg gtttgggggc
       61 attggcacct atattaaggg aagcgacgaa aattatcata gtgacgctgc aaatgatgct
      121 gtccgcgtga atgcaattga tgttcacgca cgcatgattg gtgagggtgc gaatcttggg
      181 gtgacacctc ttgcccggat tgagcttggg ctcaagggcg ttttgatcaa tagtgacgcc
      241 atcgataatg ccggtggcgt caattgctct gatcgagagg tcaatcttaa aatactctta
      301 cagcatctcc ctcttcaaaa gcgcgatgct cttctcgcat ccgttagtca tgaaattgtc
      361 gaccttattt taaaagataa ccaaaatcaa aatgaaacac tggatcgtat gatgctcgag
      421 gcaaaagagc atcattttga ttattgcaat ttgattcaaa aacttgagag agaggtaggc
      481 ctcaaacgct ctgttgtttt tctcgacaag gatccttttt tgcgcaacaa ccgttctttt
      541 ttaacgcgat ccgaaatggc tgttatcatg gcctatagta aaatactcct taaaaaaatg
      601 attttagaga actttgcctc ctttgaaaaa gaagcctcaa aagccattcg caattatttt
      661 cctcaaacga ttaatcagca ttttgacaaa gaaatcaaac accaccttct ctataaggaa
      721 attgcgtctc ttgcccttgc aaaggctatg attcatgaaa aagggatttt gtggctcacc
      781 attaaaaaag agagttcctt tttagagccc cctcatactt cacccacgcc tccctatgaa
      841 caatgaatga tggcgcataa accaacagcc tctacatctt atgctcttgg ctatttttac
      901 gaaacccttg cacggtggtt tttactcctt aaggggtaca aaattattgc acgacgctat
      961 aaaacaagtg ttggcgaaat tgatcttctc gtatgcaaag gcaaaacact gattgctgtg
     1021 gaggtgaaaa atcgcaaaca tcaaaaagat tcctattacg cgctcacgcc ctatcaaatg
     1081 cgtcgtatta aacgcactct tctcctcgcc cataaaaaat ttccacaatt ttacacgatt
     1141 aggtgcgata cgatcttgat ttctccgtac aaatggccaa tacatattct caatgctttt
     1201 taatctttga gagatccctc cttatgacga ccccttttag aaaatcctcc cttttttccc
     1261 tgacaatgct caccctttca ctttctcttt ctgggtgcgt tcctgttctt gtggcgggag
     1321 caggtgttgc aaccgttaca tccgccgcca aagaaaaagg aattagtggt ggcctcagcg
     1381 atacacaaat ttctgtgagt atcaaaaaga aagtctatga aaaagatcaa gatattcacc
     1441 gccgtattgg tgtgaatgtt caaaatggcg aggtgctttt aacgggaagt ctgccaacat
     1501 ccgaactgat cgatgatatt gaacaaattg cctggggcac acccggtgtg aagaaagtga
     1561 ttaatcagat tggtatcagc aatgacgata gtttcggcct aacccaaggc acaattgata
     1621 gctggattac aacccaaatc aaaagctccc tcacatttac aagcgatatc aaatccatta
     1681 actatagtat taaaacggta agtggtattg tgtacgttat gggtgttgcg caaagccaag
     1741 aggaacttga caaagttctc acaacagcac gcaacacgcg gggtgttaaa aaagttctga
     1801 gctatgttaa aataaaagag tctcctgtgg atgttcccag tggcaaagcg acttcgaaag
     1861 aagatgcctc tgaagagcct gccgcagaag ctgaataata attgtgctca atagatagaa
     1921 aaagtttgaa ggattagaaa atggccggtc actcacagtt taaaaatatt atgtaccgca
     1981 agggtgctca agatgcgaag cgggccaaga agtttgccaa aattacgcgc gaaattatgg
     2041 ttgctgtcaa aacgggcaac cttgaccctg agtataaccc acgccttcgc tcggccctcg
     2101 cccttgcccg cgcagccaac atgcccaagg ataatattga ccgcgccatt aaaaaagcct
     2161 ctggcaacga tgccgatagt aactttaccg aggtacgcta tgaaggatac ggccctggag
     2221 gggttgctgt ggttgttgaa gccctcaccg ataaccgcaa tcgcacagcc tccgaggtgc
     2281 gtgctgcctt tacaaaacac ggcggagccc ttggggagac aggcagtgtt tcttttattt
     2341 ttgagagagt agggcatctc cttttggcga aaaaatggtc ctttgatcgt gttttcgaag
     2401 cagccctcga tcaaggtgct gataatgtgg aagaaacgga tgaagggatc gaggtcacat
     2461 gtgctgtgga tgattttgcc gccgtccgtg atggtctcac aaaagccata ggtgaacctg
     2521 acagcgctca gcttatttgg cgccccctca ccttggcacc cgtcgatctt gatacagcca
     2581 aaactctttt aaaactggtt gatatccttg aggataatga tgatgtgcag cacgtttaca
     2641 cgaacgctga tatttcagag gagctcatgg cgcagcttta aaaactgcgc tccttacgtc
     2701 tttttatttt atattaagac gtaaagagtt aagcgtgttt tgactctttt aggcacagct
     2761 ttaatcccat gggactatga cgttaaaagg gcaccgcttt cgggtttcca ataaaggtaa
     2821 acctcgtcgt cccaattcac atcacgctca ttcaaacgaa attgattggc ctgggccgct
     2881 tgaacaatct tacccgagtc aagggccacg tgataaatcg acatatcccc aaggtagcca
     2941 atttctttga caattccctt aatgcagttt tgttttgcct tggctggcaa tgtgcgagaa
     3001 ataatcattt tttcaggccg aatcgccaca gccacatggg aaccaatcgg cacacccggt
     3061 gcatgcgtca cataaatatt gcaccccgca tccatagatt caacaacagc atgatccgcc
     3121 tcatcctcaa ggataagccc ctcaaaaata ttcatgctcc cgataaattc tgcggtaaac
     3181 ctggaatttg gaaactcata aatctcgtaa ggcatcccga tttgacgaat atgcccctgc
     3241 tccatcacgg ccaagcgcga ggaaatggtc atcgcttcct cttggtcgtg cgtcaccatc
     3301 acaaaggtca cgcccacaga ttcttgaata ttcacaagtt caaattgcgt ttgttcacgc
     3361 agacgcttat caagcgcccc cagtggctca tcaagcaaaa gaactttggg ttgcttgaca
     3421 agggagcgtg caagggcgat acgctgtttt tgaccaccac tcaactgatg cggtcgccgc
     3481 ttggcatagc ctgtcatttg cacaagatgc aaaccatgct caacgcgctc tttaatcaca
     3541 gaatcactca atttttcctg acgcagacca tacgcaatat tttgctcaac agtcatatga
     3601 ggaaaaagcg cgtacgactg aaacatcata ttcaacggcc gctcatacgc cggccagtcc
     3661 gtaatatcaa taccgtcgag gtaaatacgc ccctcatccg gctgttcaaa tccagctaaa
     3721 aggcgcaaaa ccgttgtttt tccagaaccc gaccccccaa ggagggaaaa aaactcattg
     3781 cggtaaatac tcaaactcaa atgacgtaca gcctgcacat taccaaaact tttggacaca
     3841 ttatcaagaa caagatacgg ctttgtgttt gggtcttgcc aaggctccaa agcacggtca
     3901 tgggggcgta aacgcggtga ggaaggcgcc atcaattcac tcagtgatta aaaagtagaa
     3961 cttagacgtc atcttaatcg ttttttgcga taaaaaatat gattttcagg cgatgatttc
     4021 taattgtgag ttttagactc cctcttcttt tttacctttt cattctgctc aattactcta
     4081 tccagttcat ctaaagcatc tgaacaacaa cactcacaaa agcactgaac atactcaaaa
     4141 atagaggaga aaacagagga agattgattg ttctccccat tatagtaaag atttttagga
     4201 ttccgattgt tatcaaaatc ctgatatgtc gtcctatagt cggaggcaac ggagtgtgaa
     4261 aaagaaaaaa cgagtaaact gaagataggc aaaaatttca taatagcttt ctaagattta
     4321 aaaatgaatc gcatgcccat cggacgcaag agccgcctcg tgcagcatct cactcagcgt
     4381 aggatggggg aaaatcgtgt gcataatatc ttcctccgtc ccctctaatg acttacaaag
     4441 cgccaatgag tgaatcagtt ctgtcacctc cgcgccaatc atgtgtgcgc ccaaaagctc
     4501 ccctgttttt tgagaaaaaa tgagcttaat aagcccctca ccctccccca tggcgagtgc
     4561 ttttccgttt gccataaaag gaaaacgccc aattttaagc gtatcgcctt gtgcttttgc
     4621 aaaggccagc gctttgtcct ccgcaagacc aatactcgca atttgcggca tactataggt
     4681 acagccagga atattttctt ttttgatggg ctttgtttta agacctgcaa ttgtttctgc
     4741 cgctaaaacc ccctcgtggc ttcctttatg cgcaagccac ggcgcgccca caacatcgcc
     4801 cacagcaaaa atagtcggct cctttgtccg acaccagtca tcaattttaa tatgcgcgcg
     4861 atcaagttct atttttgtcg tctcaagacc aagattttcc gtattggcca caattcctat
     4921 ggccagcaag agtttatccg cctcgagtgt aaccgtttta ccctcttttt taaggtgaac
     4981 ttttatgcct ttggcgcttt cttccacttt gtcaaggcgt gtgctgaggc aaaaatcaat
     5041 cccttgtttt ttaaaagatt ccagcgcaaa ttttgaaatt tcggcatctt cagcatttaa
     5101 gatacaatcc aacatctcaa cgactgtcac tttgacacca agggcattat aaaaagaagc
     5161 aaactcaatc ccaatcgccc ccgatccaat aatcacaagg gattttggaa gagctgtcgg
     5221 cgtcatggcc tctctggctg tccaaatatt tttcccggcg gagagattcg gcagagtacg
     5281 ggccctggca cctgtggcta aaataatatg cttgctttgc agtgtttctt tttggccatt
     5341 tttatccacg attatttttg taggaccgtc gagacaccca tgcccttcaa tgtgcgtgac
     5401 accatttttt ttaagaagat gcttaatacc gcccgaaagc ttcccactaa tgtcacgcga
     5461 gcgcttaaca atctttggca gatcaatgct agaatgttga acattaagac cataatcact
     5521 ggcatgatcg ataaggtgct tgacttcggc ggagcgcaaa agtgcttttg tgggaataca
     5581 tccccagtta agacacactc cgcccagatg ctcacgctca acaagagccg ttttcagctt
     5641 gagctgcgca cagcgaatcg ccgcgacata gcccgcagga ccgcccccca caacaacgac
     5701 atcaaacaat tgcatagaaa aaaccttatt aaaatccaaa gactcggtga ttttaacaag
     5761 gttttaaaag aagagtaaag atctcaaacg tcaaaaatta acgaagatcc cgggttaaaa
     5821 gatttcccca aaaattttca agctttttaa gcatgtgccc taaatgctca atcacatttt
     5881 tatcaccact atttttctca agatggtgcg catgagcggt caaaatctta tcaagttttt
     5941 gaaaaagatc cacacccttc gccgaaagct tcacatgact ggagcgacga tcgtggggag
     6001 aaggctcttg attgatataa ttattttgaa ccatctttcg aagattgtac gaaacattcg
     6061 acccaaggta atagccacgg ttcgtcaatt cacccacagt gacttgatcc tggccaatat
     6121 tgtaaagcac aagagcttgt acgttattaa tgtcgcgaat gttcatgcga tcaagttcaa
     6181 ctttaatcac ttctaaaaaa aggcggtgga gtctctcgat catcaccaca gattcaaaat
     6241 aactctcttt catagcatcc tctagggcgt aagaattgta tcctacttac agttttaccg
     6301 caagtaagtt aaaatttatt taacaaaaac aacaattttt ctttcaaatg aaaaaaaatt
     6361 ggattagaat ccatgattta tttttataga attctaatga tcccgcttaa gcgccggaaa
     6421 cgtagacaca ccacgctcga agcatgtata ctacactcgc tttaaaaata ataggtaaaa
     6481 ttttatgaaa cctgtcattg gaatgacact cgattgtgaa accgctggag gttatgccag
     6541 tgtgccttgg tatgccttgc gcaaaaatta ttgtgatgcc gttgcgcacg cgggaggcat
     6601 ccctttacct ctcccccatc agatcgatgc tgtcgaagcg tatgcagacc gccttgatgg
     6661 ccttgtgctt acggggggtg gctttgatat tccccccgag ctctatggtg aaacaacgat
     6721 tcatccgact gtcactctca agccagaacg cacgcatttt gaactggcta tcgcaaaacg
     6781 ttgttatgaa caaggaaaac ctatttttgg gatttgcggc gggatgcagc tgattaatat
     6841 tatgttcgga ggaacactta ttcaagatat tcacagcgaa tatatcagcg caggcgcaca
     6901 aattatcgaa accaatacct ttggcgcgaa ccgggcaaaa cttgaagcct acaatctgga
     6961 agagcaggtg cgtgagatta ataaacgcgc tgcgaaa
//
LOCUS       DAIAHG010000051         6913 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_51_length_6913_cov_3.141018, whole genome shotgun sequence.
ACCESSION   DAIAHG010000051 DAIAHG010000000
VERSION     DAIAHG010000051.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51_length_6913_cov_3.141018"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2011 a         1327 c         1586 g         1989 t
ORIGIN      
        1 atcataaaag gtgatctctt ttatctcacg cgtgtaatgt cgtatctttt ttgtaagtgt
       61 gtgtgtatgt tcaaaaaatt tttgaaaagc attccccttt tgaagggaaa aaataagagt
      121 aaaaaggatc aataaaattt tcataaacga cccagcttga ttttgataaa atgtttttaa
      181 aattctagca gtagaaaaat cgtgcagcaa agggtcattt tgctttgttt gaaaatctat
      241 tatgtttaag aacactttct tgtggtacaa aaaactatat tttcctgagg atagagatgt
      301 tttatgtctg agtttatcgc tgaagcgaag ttgcgcggct ttgtgtatca atgcacggat
      361 gaagaggggc tttcggcggc tttaaaatcg ggaacagtca cagcctattg cggttttgat
      421 gcgacatcga caagccttca ggtggggaat cttgtcgcga tgatgtattt gcgtttgctt
      481 caaaagcatg gtcacaaacc cattgcgctt atcgggggga cgacaacaaa aattggcgat
      541 ccatctggca aagatgaaag tcgcccaaca atgagtgagg agcagctcgc ggcaaatatc
      601 aaagggattc gccctagttt cgaacgattt cttgattttt ccaatgaccg ggcgatctta
      661 gcgaataatg atgcgtggct tggatccctt cattatgtgg attttttgca aaccattggg
      721 cggcattttt ccattaatcg catgttgagt tttgattctg tgaaactacg ccttgagcga
      781 gagcataatc ttaccttcct tgagtttaat tatatgattt ttcaagccta tgattttctt
      841 gagctctaca agcgttatgg gtgtacatta cagcttggcg gatcggatca gtggggcaat
      901 attattaatg gtgtggagct ggttcgaaaa atccttggta agacagttta tggtcttaca
      961 gcgccgctga ttacgacaag tacaggggct aaaatgggta aaacagcctc tggcgctgtg
     1021 tggctcaatc atgaccgctt gagtgcgtat gattactggc aattttggcg caatacccac
     1081 gatcttgatg tgatgcgtta tcttaaaatg ttcacagata tgccgcttag cgagattgct
     1141 cgttttgaga gggctcaagg aagtgagctc aatgaggtga aaaaactttt ggccgacgaa
     1201 gctacaaaac ttaatcatgg cgttgactgt ctcgtggata ttcatgcgac ggttaagtca
     1261 ctctttgaaa aaggcgaggg cggggattta tcaagcctac cagagtatgt gattgatgcg
     1321 ttcccggaaa aaggtatgag tgttttagac ctttgcgtgc ttgcaaaact caccacaagt
     1381 aaaggtgagg cgcggcgttt gatggcgggc gggggccttc gtgtgaatga tcatgttgtg
     1441 gtagaggaat taatgtactc aaaggatcaa ttgcctttga aattatcagc aggtaagaaa
     1501 aaacatgcac gggtgatggc taaacattaa aaagatagac aaaaaaagag gatcataaga
     1561 ccctctctct ttatatttta aaaatcactg tctaaccagc aagttgtgct aagggttttt
     1621 gggccttgag atcaccaatc attaagggtt catcttggtt ttcaggatca cgcaagacat
     1681 aacccctgcc ccagactgtt tgaatataat cgtcattctc gagagcctcg gcaagcttgc
     1741 ggcgcagttt gcaaataaac acatcaataa ttttaagctc tggctcatcc ataccgccat
     1801 aaagatgatt taaaaacatt tcctttgtga gggtattccc cttacgcagc gaaagaagtt
     1861 ccaaaatggc atattctttt cccgtgagat ggagggtcac accatccaca ctggctgtgc
     1921 ggctttgcaa atttacaagc aaacgccctg tgcgaatcat agattctgag tgtccgcgag
     1981 accggcgcac aatagcatgc acgcgcgcta aaagctccac acgattaaaa ggtttcgtca
     2041 ggtaatcatc cgcgccaaac ccaagcccct tgaccttatc atcaatctca gaaagaccgg
     2101 agaggataag aatcggcgtt ttaatttgcg cagccctaag gcgtttgagc acctcgtagc
     2161 cattaatatc gggcaatacg agatccaaaa taatgacatc atattcataa agcttaccaa
     2221 tttctaaacc gtcctcccca aggtctgttg tatccactac aaagccttct gagcgaaggg
     2281 ttgtctcaag aacttttgcc gtcgttgagt catcttcaat taaaagaatg cgcatgagat
     2341 tctctctcct gttaacaatg cttaaggata tgctaacaaa aaaatagcga aatatctaaa
     2401 taaatattta atttttttta gctaaaaatt aacgatttgt ttactttttc catgtatgtg
     2461 tcacacttga aaaaacaaag acacgggaag gaaggtagtt ttatttatga ctttttcaag
     2521 agcattagat catttaaaaa cgtctctcga tgcctccaag gcttatactc tttttggaaa
     2581 aatcattgat attcaaggca tgtgcgttgt tggatccgca gatcacccct cgatttgtat
     2641 tgcttctcag gtgattattg taaaagatac tcactctttt tatggagagg tggtggcgct
     2701 taaaaataat caagcaacga tcttgcttgc aacgccgatc atggggctga gtatcggtga
     2761 tcccattcac tataagggag gtgcgtctgt tatttatccc tccgctcaat ggctggggcg
     2821 tacgctgaat gcttttgggg aacctttgga ttctttggga ggcatgccaa aggggtctat
     2881 ggcgatccct ctcaaaaaac aaccgctctc tgctattagg cgccgaaaag ttaatcaggt
     2941 tatggatctt ggtatccgtg caatgaatag ttttacccct gtgtgccaag gacagcgtct
     3001 tggtattttt tcaggttcag gggtcggaaa atctgttctc atctcgatgc ttgccagaaa
     3061 tacagcatgt gatgtgtgcg tgattgggct gattggtgag cgtgggcgtg aagttcgcga
     3121 atttatcgag gatacgcttg ggaaagaagg ccttcaaaaa agtattcttg tgattgcaac
     3181 gtctgatgag ccgccccttg ccaggcgtca agccgcgtac atggcgctaa cgattgcgga
     3241 atattttcgt gatcttggca aaaatgtttt atgtatgatg gatagtctga cgcgttttgc
     3301 catggcgcag cgagaaattg gtctttctgc gggtgagcct cccacaacaa aaggatatcc
     3361 tcccagtact ttttttgagc ttgcgcaact gtgtgagcgt gcaggtcctg gccttgacgg
     3421 gagcgggatg atcaccgctt ttttcagtgt tttagtggac ggggatgatc ataacgagcc
     3481 gatctcagat gcagtgcgcg gtattctcga tggtcatatt tacctggatc gcgcccttgc
     3541 tgaacggggg cactatccag ccattcatgt gctaaaaagt atttcacgcc tcacatcaac
     3601 cgcgctcaat gatcatcaaa aagatctttt gcaacaggcg aaaaaaaatc taagtgccta
     3661 tgaagatatg gcggagctta ttcggattgg cgcgtatcaa caaggcagtg acgctgatgt
     3721 cgatcgtgcg attcagcttt atccctcttt ctttgaattt ctgacgcaaa agccagatga
     3781 gcgcacgtct tttaacgaaa cttttgaaag gcttgagggc atcttaggga ggtaaggtaa
     3841 cattttatta agaggccttc ttttaatata aaaagagact cacgtttatt ttggatatat
     3901 cccatgacga aaattatttt cacgctcctt tttatttttt gtcttgggca tgaggctgcg
     3961 caggcatccg aaaagaagtt aaaatttgag gatatacaat ttaatttgtc atccaaagag
     4021 atcaaaagaa gtaacgggac tattcacgtg actaaatatg atttgaaggc caaaaatcaa
     4081 gacatcggtt ttttacagat tacatgccgt ccagaaacgg aaaaacagtg ttcgcttcaa
     4141 acacacttta atgaaaattt tcgaaatcaa gggctgagcg cttctattca aaaagaatgt
     4201 atctcgttgt tccatcagtt tataaaagag caactcaaga gcgataaaat tatttatgct
     4261 gaagttagct ttctgaacta cccctccatc aaaaaaaatc taggtgctgg aatgaaaatt
     4321 tatggcatag atgaggcggg gagttttatt tttgtggatg atttagacaa agcaaaacaa
     4381 tatctttcag aaaaagataa aaatgaatat aaaaatgaca ttcctcatga ttggagcaag
     4441 aatatgcaga cgattcttca agagcaatac gagagggaga gcgatttttt taaaacgagt
     4501 attgaattgg ccacctccaa aaaatatctt gccgagcata aaaatattca taatgccgat
     4561 gattatttta aaatgatcaa aaaaattgcc aaaaataatg gtattctttt tgagggagag
     4621 gacgaggaaa aaaagaatta gggaggacga tgcagattaa aactcaaaaa gcccttcaaa
     4681 ccctgctcaa ggtgaaaaca gcagatcttg agcaatgcct caaagagcgg caagaaatct
     4741 ccctggtcat tgacatgctc caccatgaaa aagagctcac tcaagagcgt cttgataaag
     4801 aaaaagcctt tcaaagtgac cccagtgcca cagcgacgct gcatcatttt gccaaggcgt
     4861 gcgatgataa aataaaggcg attgatggag aaatcgcatc tcacttagtg gcgttggaca
     4921 agctccaaca cactctttta gcgctccacc gtacgataaa acgttttgaa tttttgcttg
     4981 aaaaggaggc gctgaatcgt caaaaagagg caaaaaaaga ggagcaaaaa acccttgatg
     5041 agatcgctct tcggcgattg agagggtaga cgtaggaaaa ttttaaagat tgctgggaca
     5101 tacaagcgcc taatatatat tttttataca atagattttt taacatttta ttaatgattg
     5161 ttttgtatca ttgattatat aaaatttaaa gtatttttgt tatgctgaca acgctggcgc
     5221 ataaacttga aaaagcagct gaaaatgttc cttttatcaa aacagaaagg cttatgctgc
     5281 ggccttttac ttttcaggat gcacacgata tttttgagta tgcacgtgat gagctaacgg
     5341 ctctttatac gacattttcg ccgcataaat ccctcgagga cagctattat tttttatatg
     5401 aaattgtttt gccttcttat gagcgaggcg accttgggcc tcttgcgctt gatttaaatg
     5461 gaaaagtcat tggagcgatt gaactgcgca cgattccgtc ccctattcgt caccacaccc
     5521 gggaaatggg gataattctt aatttgcact atcgtagcaa gggctatatt cacgaagctg
     5581 gaaaggcgtt aattaaatat gctttttccc aacctgggat ttatcgtctt tttggatgtg
     5641 tagaaatgga taatcatgca tcagtaaaag ccttggaaaa gcttggcttt tcttatgaag
     5701 gaaaagaaat tcatgggcgc atcctcaaaa gtcattacgt ggatctgttg cactatagtt
     5761 tgctaaaaaa agattggtca aaaaaagatt caaaacaata atcactctct tttgcttgag
     5821 gtgagtgcat gtatttcctt gagaaacctt gcccttttga aggggattcg ctaggtttag
     5881 gtgtataact tttctgtatt atttttgaaa aacatgtttg atcatttaac aaagcgcctt
     5941 gggtctgttt ttgaaaaact taaaggccgt ggcgctctca cagaagcgga cgtaacagag
     6001 gccttgcgtg aagtgcgcgt ggcattactc gaagcggatg ttgctcttgc gacggtgaaa
     6061 tattttattg aggatgtgaa aaaagaagcc attggcacgg acgttctcaa atcgattaca
     6121 cctggtcaaa tgattattaa aattgtgcat gatcatctgc ttaaacttct tggtgaggag
     6181 gtcgtccctc ttgattttca ggcagcgccg ccgattgcgt atcttatggt ggggcttcaa
     6241 gggtctggta aaacaacatc ctcagccaaa cttgcaaaat atatcaaaga taaactgggg
     6301 aaaaaacccc tccttgtctc tttagatgtg tatcgtcctg cagcgcaaaa gcaattaggc
     6361 atattggctc aacaacttaa cttagacgcg ttggacatta tcgaggggca aaaacctctt
     6421 gagattgcaa agcgtgcgtt tgaggtcgct aaaaaacaaa gtatcgatgt gattattttg
     6481 gatacggcgg gtcgtctgca tattgatgat gccctcatgc aggagattcg ggagattcaa
     6541 aattatagtc atccgcgcga aacacttctt gtggctgatg caatgacggg gcaagacgct
     6601 gtgacgatgg cggatgcgtt ccacaaaact gtaccgttga cgggtattat cctcacacgc
     6661 cttgatggtg atgcgcgtgg tggtgcggct ctttccattc gtcacgtaac gggacagccc
     6721 attaaattta tgggcgtggg cgaaaaactt gatcagcttg agctttttga tcctcgtcgc
     6781 gttgcggatc gtattttaga tcgaggcgat gtcgttgccc ttgtcgaaaa agcggccttg
     6841 gcgattaatg aagaagacgc caagaaaatg gcggataaaa tgcaaaaggg gcaatttgat
     6901 ctgaatgacc ttg
//
LOCUS       DAIAHG010000052         6891 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_52_length_6891_cov_3.378192, whole genome shotgun sequence.
ACCESSION   DAIAHG010000052 DAIAHG010000000
VERSION     DAIAHG010000052.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6891)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52_length_6891_cov_3.378192"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1918 a         1560 c         1408 g         2005 t
ORIGIN      
        1 catcaacaat cgttagacca tgactctctc ccacttttgt aaagcgccgc ttcacacctt
       61 tgaaatggct aagtgctttg atcatgacaa catcacttat cccaagctct tgcgccacag
      121 caatgacagc caacgcattt tgcacattat gctcacccat cattggtaaa aagaaaccgc
      181 gaatacgacg cggcattaat gtcagcgttg ttaagcttgg ctgaattgtt ggcggattaa
      241 tatccacatc aaaggtaatg ccttcttctg tggcatgaag attgacggca cgaatgtgcg
      301 ccccctcctt gagaccataa gtcacaacgc gtttgccgat aagatctgga attatttttt
      361 gaacttcctc atgatccgcg cacaaaatag cagcaccgta aaaaggcaca cgatctaaaa
      421 agcgcataaa agccgatttt atctcgctaa aatctttata aaaatccatg tgatcttcat
      481 cgatatttgt gaccaccgca aatgttggaa aaagtttgac aaaactacca tcggattcgt
      541 ccgcctcagc aatcacccaa tcccccgtac ccaagcgcgc attcgtatta taggcgttaa
      601 ggataccccc attaataatt gttggattaa gttccgcttc gtcaaaaaga gccgccatga
      661 gagacgtcgt tgttgtttta ccatgcgttc cagcaatggc gacagcgaat ttaagacgca
      721 tcagctcgcc tagcatttct gcacgttgta caaccgcaat accttgattt tgcgcagcga
      781 tgacttctgg gttattttta gacacagccg ttgaaacaac aataacctga gcgccctgaa
      841 tattctcaac cgcatggcca atgtgaattt taatcccaag atttctaagg cgctgaatat
      901 tagagctttc gttcgcatca ctccctgata cgaaataacc aaggttcgat aacacctccg
      961 cgattccact catgccaata cccccaatgc ccacaaagtg aatatgccgt atgaaatgag
     1021 gagaaatgcg catggtgcct ctataactat ttaattgttt gtctctaatg gtggtagcaa
     1081 atcttggatc acttgtgcaa gatgtgatgc tgcttgcacc atactataat tttgaatatc
     1141 cttggctttt ttttcaagta tttcaggctg atttaaactt ttttgcagaa tttctgcaag
     1201 cttttgaggg gtaagatttt tttgctcttc taaaaagcac gcatcgccca aagcctttgc
     1261 attatacaat tgatgatgat ccgtcgcata aggatagggg ataaaaagtg ctgcgcgccc
     1321 tgcaatagtc agctctgaga ctgtagaagc tcccgcgcgc gctatcacca aatccgcctt
     1381 ttcataaagc tcctgtacat gatcaaaaaa aggcttgagt gttacatgca taccaagatc
     1441 atcataagat ttttgtgtgg attccaaaag ctccgcacgg cattgctgca ccaatgtaat
     1501 attcttacgc ttctgtgccg ataaaagtga tagagcttct ggcaaaaggg ttgagaaaaa
     1561 agcagctccc tgactgcctc ctgtggcaaa aagactgaga tgattctttt tcggttgata
     1621 gggcgtgcag ggatgaatac gggcgggatt acccgtcaca caatgagaaa tccttgtatc
     1681 aaaggccatt gtcttttcaa aggagagagc aacatgtcgc gcgcgcttaa gaagagagcg
     1741 attcgcacga ccaatataag cattttgttc gtggagaatt gttggaatgc cgagcatctg
     1801 cgccgctaaa acagcgggca cacttggata gcccccaaac cctacgacaa tagagggttt
     1861 ttctgttaaa aacagataaa agcaccgtaa aaaactccaa aacatcgcga aaataaaaag
     1921 aggaacttgt attttttttg agcgccgtgg gataaaaagt ttgatggcat tcacgtttgg
     1981 ctcgctcact cttttatctg aaataactgt gatctcagta tctgtcctag cctttaaaag
     2041 cgcgcgcgct gttgcaatag ctgggaacaa atgccccccc gttcctcccg cacataaata
     2101 aattttaaaa agactgttgc tcaatttttt ttgcatgaac atatgtttaa ttggatagaa
     2161 tacgctatcc tacccttttt tctttttttc tcacaataac catgatgaaa aggcgcacaa
     2221 tatttgtgaa aaagctcctc acacttatga tattcttgtt tagcgttaca ctttatatgt
     2281 ttctcctgtc atagcaagtc ataaaaagga agatagtgag aagactagaa aacctagtct
     2341 taatgacagt atgacgcaaa aaaaattgat ccatgtgata tcacaggtct tgataaggct
     2401 gaactgcttc tagaacttta taatgtggct ctttatcaca acctcacatc aaaatcttac
     2461 acttttatta ctcttgatga ggcaagaaga gcgctcgaaa aagctgataa cggatatatt
     2521 gagttccttc atgacaaagt attacaggtt aacttaggtg aaaatgtcaa agttttaaag
     2581 acaactatct tcaaccgcgg ttatggagaa aatgctgcag aaaaagtgat gaaagagtta
     2641 agagaaaaaa ctaaaaatta gtagaccatc agcgtcgcac cccaggtaaa acctccgccc
     2701 atcgcaggca tcacaatcac gtctccacgc ttaatgcgcc catcttttac cgcaacatca
     2761 aaggccaaag gaatcgtcgc agccgatgta tttgcgtgct ctgcaatcgt tgaaatcacc
     2821 ttatccatag gaagtttgag gcgctccgca aggccttcaa taatacgctg atttgcctga
     2881 tgaggaacaa cccagtcaat gtccgcatcc ttaacatgat gatctgcaag aacacaatca
     2941 atcgcctttg ccatattcac aaccgctgct ttaaaaacct ctttcccatt catgtgcaaa
     3001 gttccaaagt gcccttggct cgctcctcct tgcgtataaa gcgcatcctt aaaagcacca
     3061 tccgcataaa gttttgtgcc caaaatgcgt gacgtatctt ttgaatctgt ggccttgaga
     3121 ataaaggccc ccgcgccatc gccaaaaagc acacaagtgc tccgatcagt ccaatccaaa
     3181 agccgcgaca ttgtctctgc ccctatcaca agtgcacatg tactttgccc tgactttata
     3241 tacatatccg ccgtgttaag ggcgtaaata aaacctgaac acacggcttg gacatcaaat
     3301 gcggcacagg gagggatacc aagggctgtt tgcacaaggg ctgctgttgc aggaaacgta
     3361 taatccggtg ttgttgtcgc cacaatcaca agatcaatgg cctcgggagt tatctgagca
     3421 tgctcaagag ccaacctcgc tgcttttatt gcaagatgag acgtcaactc tccctctgcc
     3481 gcaatatgcc gtttagcaat accaacacgg ctgcgaatcc attcatcaga ggtttctaat
     3541 gtctgtgcaa ggtcattatt tgtgacaatt ttttctggaa gatacgctcc aacgccgata
     3601 acttttgatg caaccatgaa tgacgtcctc tcgtttttta atcgttagac ttaaataatc
     3661 cttcaaatgt ttttgtagtt taaccatgtc atggaaaaga gcaacgagat tttatctttt
     3721 taaaaggggg tgcccccgat taattttatt gaaaaaaagg ttatagccac ctcttccccc
     3781 atccttttag ttctttttaa ttttttcatt cgccttatcc acaagatccc aaatatcttt
     3841 aggcaagaaa tcctcttgag ttttcaaaaa ttcataattc tcctcaaggc ttgcctcagg
     3901 cataaagtta acaggttttt tcgttttttc tgtatcgatg tttattttta tgtcgcggca
     3961 gttaactagt ttttcaaaaa gaaagtgcgc catctttcta aaaaacccat catattcatt
     4021 caggccttct tttacaatga gtggggcttt gctgtcatct ttgcacaaag tagaggtata
     4081 ttttgtacaa agttctcccg tctcctcaaa gttttcaggt aatttctcgt aaacaatttt
     4141 gaatccgcta cgatacttaa gtgtgtaaaa ttgtgaagaa tccccatggc gtgtaaagaa
     4201 aatcccctta tcattaatgg caagatcgac atatgccgta aaattttttt caatgtgttt
     4261 ttcctctaag tttttttctc ctgccatgac ttcattgatg gaggaaatac aaaagaacac
     4321 agcacttatg aaaaataata ttattttatg aggcatttat tgaatccttt tttattatga
     4381 acatgtcctg tctaaatgat tgcagcacac tttgcaagat ctgatgcaat cagacgcttc
     4441 attcaccctc gtcaatctcc acaaattatg atagtttaac gtaaatttta taattaaagg
     4501 gtgcttgcct tgaagattgc tgttgctaaa gaacattcac cttacgaatg ccgtgttgct
     4561 ctcacgcctg aaggtgtgaa aaaatgcatt accttggggt ataacgtttt tgttgaaaaa
     4621 aatgcaggcg agagggccgg ttttgatgac gatgcctaca aagcaagcgg tgccatcgtt
     4681 gaagaagatc tttcaaagct ttttggtgat gctaacataa cactccgtat tcaaccccct
     4741 tcggcgcagg agatcgctta tctccccccc catagcagtc ttattgggct tttaaaacct
     4801 catatgaaga atgatgagct gaatgccctc gcccgtgaaa aagttacatc ctttgccctt
     4861 gaaatgattc caaggattac acgcgcgcaa agtatggatg tgctctcctc tcaaagcaat
     4921 ttagcaggat accgtgctgt cattgaaggt gcccatgcgc tcaatcgcac atttccgatg
     4981 atgatgacac cggctggaac gattgcaccg atgcgtgttc ttatccttgg tgcgggtgtt
     5041 gcaggcttgc aagccattgc tacagcaaaa cggttagggg cgattgtctc agcttttgat
     5101 gtacgttctg ctgcaaaaga acaagtagaa agtcttggcg caacatttat tgcaatcgag
     5161 gctgaagagt ccggtgatgg ttccggtggt tacgccaaag aaatgggtga cgcttatcaa
     5221 aaagctcaaa aactcaaatt gacagatgtt ctgaaaacac aagatcttgt gattaccaca
     5281 gctcaaattc caatgaaaaa agcgcctatc cttatcgatg aggatatggt cagtgtcatg
     5341 aaaagtgggt caatgattat tgatcttgcg agtgaaactg gaggaaattg cacgctcaca
     5401 aaacatggtg agactgtcac acacggtggt actcaaattt atgcgccact caatatcatg
     5461 agtgcgattg cctatgatgc gagtcagctt tttagtcgta acaatgtgca ttttatcaca
     5521 aatcttatca aagaggggca gattcagttc gacgatgaga ttgtcaaagc gacgtgtctc
     5581 acacatgaag gtgcgattat ccatccgctt ttcagcgaaa aaagaaaagg agattaaatg
     5641 atgcaaccga ttatggataa aatgaatgag ctgacaaaag ccatgcaatc tttgcttcaa
     5701 cagagcagtg atattcagca gtcactgggt catatccaag cgatctcacc tcacggcacc
     5761 ttagatcctt tgatgatcgg actcaccgtt tttgctcttg cgtgctttgt cggatattac
     5821 gttgtttggc gcgttacacc agctttacat tcgcctctca tggctgtaac aaatgctatt
     5881 tccagtgtca ttatcgtagg cgcacttttg gcggctggac ctcttgaaag tagtgctaca
     5941 ggcattggcc ttttggctgt attcctcgca tcgattaata tttttggagg atttgttgtt
     6001 acaaaccgta tgcttgctat gttttcaaaa agcaagaaat agcctataaa aagggatttt
     6061 tatacaatgt cagtgaatat ttcagctttc ttttacctcc tttctgccct ttgctttatt
     6121 atggcactgc gcgggctttc ttctgcagcc acatcaaaaa atggcaatct ttttggcatg
     6181 gttggcatgg gcattgccat tgcaacaacg cttttcagtc ctgttgtcgg tcactatttt
     6241 cccattcttg caatgatcgc tgcgggagga cttgttggta tttttattgc aaaaaaaata
     6301 aaaatgacag ccctgcctca acttgtggcg ggctttcata gtcttgtggg actcgcagct
     6361 gtgtgtgtgg ctttcgcagc ttatctcgat cctacagcct atggcattgg tataaagggg
     6421 catatccgaa cgatgagcct ttttgaaatg agtcttggcg ctgcgattgg tgcaattaca
     6481 ttttctggct cgctcattgc ctttggaaag ctccaagggc ttatctccgg aaaaccgctt
     6541 gtgtttgaag gccagcacaa acttaatgct gttttaggaa gcgttctcgt tctcagtatt
     6601 cttcttttta ccctgacggg ccatttaact tttctttatc tcttaacatt tatcgccttt
     6661 gcactcggat ttctcatgat tttgcctatc ggaggcgccg acatgcccgt tgttgtttcg
     6721 atgttgaatt cttattctgg atgggcagcc gccggcatcg gctttacgat cgacgccgaa
     6781 gaggccgacc gccttgaagt atcgctcgat ctcgtcgagg cgctggcgct cgcccccgag
     6841 cttgccggct ggaacgggct cggtcttgcc gtccaggcct accagaagcg t
//
LOCUS       DAIAHG010000053         6816 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_53_length_6816_cov_3.152693, whole genome shotgun sequence.
ACCESSION   DAIAHG010000053 DAIAHG010000000
VERSION     DAIAHG010000053.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53_length_6816_cov_3.152693"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2000 a         1405 c         1311 g         2100 t
ORIGIN      
        1 cattctttgg gggcttagcc cttttgtcgc aacacttctt gctgtgctcg caggagcttt
       61 tgcaggttat atcacagcac ttctcgccac acggcttaaa atgctcaatt tgcttgcggg
      121 tattttaaca atgacggcgc tctattctat taatttaagg attatggggc gaccgaatct
      181 tgcgcttctt ggggaaaata ctttatccac acagctcagc acttttgtca caaaagtcct
      241 aggacttcct gcaattctca cagatcatgc gaatttaatg gggcttgcgc tgattacact
      301 tttggtcgtt attggacttg agtggctctt aaaaacacgc tttggtttag ccttaaggtc
      361 tattggttca aacccacgga tggcgcgctc gatcgggatt aatgacgatg ctatggtgca
      421 gtcaggcctt gcgctaagta atgcgcttgt ggcgcttgct ggcgctcttt ttgcacagta
      481 ttccgggttt gcggatgttt ctatgggcgt tggaaccatg attagtggtc ttgctgctgt
      541 gattattggt gaagcgcttt ttcatacgcg cagtgtcgca aaggctctta tcgcgtgcgt
      601 ccttggtgcg tttatttata gacttgctat cgctgttgcc cttaattcga cggatcttgg
      661 ccttcaagcc tcagatctta accttgtcac agcttttatc tttgcgattg ctatgattgt
      721 tcctaaattt aaaatctttt tcaagagagg aagagacaaa aaatgatcat cctcaaaaac
      781 atcaaagtta cgtataaccc tggctctatg cgcgagaaaa aagcactgcg cgggattgat
      841 cttaagatga atgaaggcga atttatcact gttattggca gcaatggtgc gggtaaatca
      901 acgctcctca atgcgttggc tggagaagtt ttgcccacaa gtggaagcct taaaattgat
      961 acagtcgatg tgacaaaaac accggcgcac aagcgtgctg ccatggttgc acgggttttt
     1021 caagatcctc ttgcagggac atgtgcagat ttaacaattg cagaaaattt agcacttgcg
     1081 gaacgacgtg ggttctcgcg gggtcttaat ttgtctcttt caaaagcaaa attagcggaa
     1141 tataaaacgc ttgtcagccg cctaaaactt gggattgaga atcgacttga ttcattgatg
     1201 ggctcacttt caggaggaca gcgtcaggcc attagtcttc tcatggcaac gttgagtcca
     1261 ctacaaattc ttttgctgga tgagcataca tctgcccttg acccaaaaac agcagccttt
     1321 gtgatgcaac ttacagaaag cattgtcaaa gaaaaaaacc tcacagcttt gatggtcaca
     1381 catagtctgc atcaagcact tacaacgggt acgcgcacga ttatgctcca cgaggggaaa
     1441 attatttacg acgtgagtgg cgaggagcgc caaaatctta cgcctcaaga tcttttggaa
     1501 aaatttggca gctcaatgga tgatgaccgc ctgttgctga gaaactaagc ccttagtaat
     1561 catcccccct cgtaaagaag ggggggcagt gctgcggtta ctctgtaaat ttaatgctcc
     1621 ctttgagcac atcaacaaac tgcttacgca aactgcctgc cgtagcatta gaccctgtat
     1681 taagatttac cgtcatttca tgattactat tgtcattatt cataacaata ccatgatttt
     1741 ctaatttttt ctcaatctct tccataattt ttgtatattt tttctcttgt tcatccgtcc
     1801 aatcattgga gagaattgtt ttatttgtat caaatttcaa aaaatcttta tttgaatttt
     1861 ttacaaagaa taagttgttt ttttgttctt tctcttcagc agccaaaaaa ttcaaaactt
     1921 cttttcttat attcttttca tctttatcga tagttacaga gtaaagctca ttttccctga
     1981 tcttaatttc tggaaatccc tgtatctttt cattataatc ttggattgtt tcgtcatcca
     2041 aattattttc aatcgctgtc ttcttgcaat caagataaat gcctttttcg ttatcattag
     2101 ataaaacaag ccttactttt tttccatatt cttcatcatc tgatgcataa ctgtacccaa
     2161 cgaagcgcgt gattaaagtg agtgctaaaa ttaatttttt tatcattatg tattcctttt
     2221 attcgatatt attttgtctt tttgacagga agcatcctga atgtttttgt cttaatagtc
     2281 aagataataa aatatgacta aaaagtaact gaataacgat cttttttatt ttatcatatt
     2341 aagttgtgca gctttgacaa cattttgcat cagacacgcc accgtcatag gcccaatgcc
     2401 ccctggaaca ggtgtaatat accgggcaag gggcgccacc tcatcaaaaa gaacgtcacc
     2461 tttgagtatt tcttccccat tttcgtgtac acgcgtaatc cccacatcca acacaatcgc
     2521 cccttttttt acatgatgac gtgtgataag acctggtttt cccgcagcaa caatcagaat
     2581 atcggcttga cgtgtaaaat gctcaagatc ttttgtatga cgatgtgtga ctgtgaccgt
     2641 tgcatgattg cgtaacatca acgcggacat aggccgcccc acaaggagcg atcttcccac
     2701 aacaacaaca tgcttagatt taatttcagt catgcgtgat aaaatctcaa gacagccttt
     2761 gggcgtacac ggaacaaaac gtggagtcga cgaaaagaga taaccaatat tttcgtgatg
     2821 aagaccatca acatctttaa gaggattaat tctttgaatc acctcgtagt tattaagatg
     2881 gagggggaga ggcatttgca ataaaatacc atgaacatag ttataattga gctcttcgat
     2941 gatgctataa agttttgctg tcgatgtatt ttcactgaga tggatcacgt cagacttaat
     3001 ccccatctcc tcacaggtga ttttttttcg gttcacataa atttcactcg caggattatt
     3061 ccccaccaaa ataacagcaa gacgtggctt gattgaaagc tcttcaacct cttttttaat
     3121 tttttcaaga agatgctcgg caagcacctt tccatgaata atattccctt taataaaaaa
     3181 cggatgatct tgcttcatac gacgtcacta ggatggaaat ttaataagaa gacggataag
     3241 aatgtcctga ataaaataaa gcgcaagcac aagaaccata ggggacaagt caatgccgcc
     3301 caggttcggc ataaaaagac gaatacgccc taaaaggggc tctgtgagac gaaaaagaaa
     3361 attatggatg aagtaaacaa cttggttata gcgattaatc acaccaaatt gctcaagaag
     3421 gctgagaata atataaataa aaacggcaaa tgtataaaaa ttaatcaccg tgatcaggag
     3481 tcttaaaagg ggcacaaaaa ggatatccat ggcatttctc aaaagggaaa gtcttttcaa
     3541 acattatgaa aagtgggtta caagcgcaag aaatttgtgc ttttttcatg attttgattt
     3601 ttccttaaag agagggagtt ttgagaatga tttttttcat aaggaggcaa gttttgaggt
     3661 ttttagtgac atctcacagg atttttttca ccaagttttt acattttcta tcctatagag
     3721 ggtgtgtttt catgtttttc atacgcattt attatacaaa atcactcact tccaaaaata
     3781 tattcaaaaa ttcctactag catgcacaca aatttgctgc gttcattgaa cgaggggaga
     3841 aatacaaaaa aaattctctt aatttactta aaagccctag aaatccccac aaaaatagtc
     3901 gcctaaaatg actcaattgt cgttttaaaa agagggaaaa aattacctca gattaagaat
     3961 ttttcttgcc tgcgccttgt ggtgggcatt atttgccaaa agctctaggt cttcaaaacc
     4021 gctatacata aagcttgtgc caaggtagtg catgccgaga tagtcacata tttgctcaaa
     4081 agtctcctcg aaacctttgg ggtaagaagt gccaaagctt gcaatcacaa aaagcttttt
     4141 cccacgaagt ttacgcccaa ggtcctttct aacggtgaga agatcattaa aacggtcaaa
     4201 gaaaatcttg tgcaatgtgc tcatactata ccaatagaca ggcgttgcga taatcaaaag
     4261 atcatgcgtt attaacgttt ctatcgttgg aataaaatca tcatctaaat gaggatgctc
     4321 atactcataa ggtgcaatgg tgagatttct cagttctatg ataggaatag tttcatcgcc
     4381 aataatgtct gaaacggctt gacgtgtatg cccgaagctt cgcgtgctgc ctaagagaat
     4441 gatcggattc atttttctat cttactttta aggataaatg gtgacaatga aaatcaaagc
     4501 catgattcac atataatttg tgggctgtat ggcgcgtata ccccgtatca agatgtattt
     4561 gagcgcattg attttcacgt gcaatgctag aaatatgatg gagcaatgtt tgagcatatc
     4621 cttttcctct ctcttttttc cctgtgatca agtcatcgat ataaagagtt tttccccagg
     4681 caagtgttgt catgatgcga aagcctgcgc atgcgaccac ttcctcttta ggcataagcg
     4741 ctatgatttc atacccttct ttttgctgtt gcatcacttg gttaatataa aacgtcacat
     4801 caggcaaata ggggcgtagc tctaaaaaaa tatcaaaaga caaagctatt tcctcaggcg
     4861 tggtaagttt tctaatgtgc atacatttcc ttttctacct cacagtttta ttcagccacg
     4921 ttacgatata ttttggatag ttcgcaaacc actcatccac atgataggtt ccaagcttta
     4981 tatctgttgc cgaaaaaaag ggaccttgct ttaaaagcca acaccaaagt gtttctaaaa
     5041 aacttttttc tctataacca gaaattttag ccgcaaaata agcctcatcc gaaaaagcac
     5101 tatgttgaac gtatggagac caggtcgtac ttttcgctgt tttcactaac tgatctttta
     5161 catcctcttg atattgcatc atccctcgaa taaataagat gggtttcgca gaagagttag
     5221 gtgggatacc tatatgtcca aagatagagc gctctgttat tttagaatcg atatccacaa
     5281 ttgccttaat acgaggatcc ttaaagccca tgatatgtgc gacattgccc ccaaggctgt
     5341 ggcccataag aataatttta tttttatcca tggcctttga aatctttcca aaatcttcaa
     5401 agtgatgcag cacataatca atatcagaaa tggcaccgct tattacctca tcatcgtatt
     5461 gatagcgata atcgcgatcc cgcgggaggt accaagcatc tttaagcgtc agtgtgaccc
     5521 gtgtcgcatc tggaaattgg acaaaatttg ctacataagg ttgatccatc gccacgacaa
     5581 tatacccctg agaggcaagc tctgtacaaa gaatcgtgta cttttgccgt tcatttccgc
     5641 gccctggaat aaaaaagata acaggaaatt tttggcctaa aaaagggtcg gcttggggct
     5701 tggcatagga caaaactttt acaccaaaca cagtactatc tttaagtgtt cccggcatat
     5761 aaggatatgt ttgcgccgaa gattgtgttg ccggataaaa aagagtcgcc atccagcgtc
     5821 gtttttcaga ccctctcagc atttttcgtg aggcatcaga aaacattata ttctcagtcc
     5881 ccacattata tggtcctgtg ggctttggaa gatccaaagt aagtgcgagg actttatgga
     5941 tcccaaaaaa actcaaaagc gttaataaaa ttaatctcac atatgcctca caataaaatc
     6001 acagcggtcc tgaggcggca ttttcggaat aaaaagtatc tcaaagccaa attttttata
     6061 gacctctatg tgcatttttt caaacacaag agcctcctca aaacttattt gacgcgcatc
     6121 cgtgttttcg ataaacccca aattttcaaa gaaaaaaacc tgaggatgat aaattctatc
     6181 ggataaaatc ctcttcactt ctgccacaag cacaggggag ggaggatacc ctaggtaccg
     6241 tgaaagcgca taagtacata aaggtgatct gtcataaaat tgcacagtgc ctctagcttc
     6301 atattggcgt tgtttttgca aatgaatagt ctcatcgata aaatgcggat gtgtccaggg
     6361 ttcaaggatg cccttttttt gtgagcttct aataatatct gtcgcagctt cagggataac
     6421 gttgtacccc aaatccttca gcgcatggag cgtagaagtt tttccactgc ccggtgttcc
     6481 tgttataatg tagcgtttta tatgcataat atttcccaaa tagaatgtta ttttaacgat
     6541 gttttgtgcg agaaaacaca tttcctagat tttaatgtca aaaaagatta gtatgagcct
     6601 atacatacac tatatctttg cgtttgcctt caatgcagcc acccggaact aagcctccaa
     6661 cgcccaaaaa atctcaaaaa aaagctcgca agaccaagcg ctcttttctg tggacgtttt
     6721 ttaagtctct ttttgtttta gctgtttggg gcatttgctt tttatgcctc gctatccttt
     6781 ggtttagcta cgatcttcct gatattcatc aacttt
//
LOCUS       DAIAHG010000054         6757 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_54_length_6757_cov_3.617365, whole genome shotgun sequence.
ACCESSION   DAIAHG010000054 DAIAHG010000000
VERSION     DAIAHG010000054.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54_length_6757_cov_3.617365"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1935 a         1522 c         1372 g         1928 t
ORIGIN      
        1 tatcgatcaa tttctttcgg cgggagaaat gaaatgggcc agacaaaatg gcctcgttct
       61 tttgcttcct cacggctttg aaggacaggg gccagagcac tcttcagcgc ggcttgagcg
      121 ctttttacaa ctgtgtggcg agggcaattg gtcggttgca aattgttcaa cgcccgccaa
      181 ttattttcat attctaagac gtcaacttaa gcaaacgtac agaaaacctc ttgtgcttat
      241 gacgccaaaa tccctcctac gtcataagct ttgtgtgtcc tctttgagtg attttgaggg
      301 gactttcaag cctgtcataa gtgatacaaa aaatcctgaa atgatcgaaa aagtcatttt
      361 atgttcgggg aaaatttatt ttgactttct cgaagaatct ttgaaaaaca atcatgcgca
      421 tacggcaatc attcgcctcg aacaatttta cccctggcca aaacacgatc tgatcaaggc
      481 ccttacgcct tataaacatg cagaaatcat ttggtgccaa gaggagcctg aaaatatggg
      541 cgcttggcac tttgttgatc gccgccttga agctatttta caccatattc aggcacatac
      601 gccaaggcct tttgtcattg cacgccccgt cgcagcgtca acagcaacag gtcttttgag
      661 tcgccatgaa aaagaacagc gccttattat tgaaaaagct tttgccccca ctcatgtgaa
      721 acccctctct gcgatcaagt aaccgacaac cctaaaggta atattttatg agcacaattg
      781 atattaaagt tccacctctt ggcgaatctg tcacgacagc tattattgcg cgctggtata
      841 aaaaagtagg cgaggctgtc catgaaaacg aaattattct tgagttggaa acagataaag
      901 tcacccttga ggttaacgct gccacatccg gtgtgcttca gcaaattagt gcttttgaag
      961 gagaaactgt agcgattggc gacattgttg gcgttattgc agaaggtgaa gtcgctgaac
     1021 ctgcacagtc tttgaaggaa agttctttta caaaagctct tgctctcgag ctagctgacg
     1081 atcccaaacc agttgaagcg aaaacagaac ctgtacaagc gcctgagacg aaaaataaga
     1141 cgataaaacc aacagaaaat ccttctaaag aagtcatcgc tagccccgct gcacgcaaac
     1201 tcatggcgga agaaaagctc acacctttgg atgttaaggg cacgggtaag gacggtcgca
     1261 tcatgaaaga agatgtttta cttcgcaatg agcgcgcgga tgtaacacct ttaggtgcaa
     1321 catcggccct tgacgcctcc gagcgaaaac ttatgacacc tttgcgtaaa aaaattgcag
     1381 agcgtcttaa atatgcacaa aatacagcgg caattctcac aacatttaac gaatgtgata
     1441 tgtcaacagt gatgaatctg cgcaaaattc atcaagatga gtttgtcaaa aaatacggaa
     1501 caaaactggg tttcatgagc ttttttgtca aagcttgtgt gcaagccctc aaagatgttc
     1561 ccgctgttaa tggtgagatt gacggagact atcttgtcca tcacaagcgc tatgacattg
     1621 gcattgctgt gtcagcaccc caagggcttg tcgtacccgt tttgcgtgat gcagatcgct
     1681 tgggccttca caatattgaa caggctattg caaattatgg tgaaaaggct aaggcgaaca
     1741 aactatccat gcaagatctt acaggcggaa cctttaccat ttcgaatggc gggatttttg
     1801 gatcactgct ttctacgccg attttaaatc caccccaatc aggcatttta ggcatgcata
     1861 aaatccaaga tcgtcccgtt gtcattaatg gtcaggttgt cattcggccc atgatgtatc
     1921 tggctctttc ttacgatcat cgcatgattg atggcagaga agctgtgacc ttcctcgtta
     1981 aagtaaaaga atatattgaa aatcctgcgc gcattctcct gggcctttga gagaaaaaga
     2041 gatttataaa aataaaatgc tcagtttcat tgcacgtttt atcgccaccg tgggctatgt
     2101 gggctatttg cccttcgctc ccggtacatg gggaagtgtt gtagcactcc ccctttattt
     2161 tttgttgcca gaatcttttt ttctcaacct tatctttcta ttttttatcc taggatgggg
     2221 atcgacctat attcttctca aaaaagatgg ttctgataag gatccttcgt acattgtaat
     2281 tgatgagctc tatgccatga tgctcacgct tatgtttccc tttcccaagc acctgagtct
     2341 atatataacc gttggcctta gctttgtgct ttttcgtttt tttgatatcg tcaagccctg
     2401 gcccattcat gtgattgatc attactttga aaaaaaaccg ccttttttag catctttggg
     2461 cgtgatgatt gatgatttta tagcggctct ctatgttttt ctcctctgct ggcttttatt
     2521 ttatgcataa agccatagcc aagccctctt ttttttggta aatttaatca aactttttaa
     2581 ggaaaattct tttatgctca caaaaatact ccttcttgga acggcttttg gcgttaccct
     2641 tgctcaaccc tctttaactt tggctgcgac agccccggat gctccacaaa gcaaagcagc
     2701 acaagcggca gacccaaaag cggatcaacc tcttgaaatg ccaaaggagc ttgagcctgt
     2761 tcccgttgac gcttctgaga aagagatcgc caaatttaca gccatgcttg aaaatgtgat
     2821 gaaaagtcca aaaggcttgg atatcatgca aaaacttcag ctagaggcac aatacctaga
     2881 ggctaaaaac gagcaagaaa tgcgcggtct tttagataca aataaagaaa aactcttgaa
     2941 tacgaaggat tctcttgttc ttggcaatcc tagtgggact gtcacacttg tgctcattga
     3001 ggaccctttg tgcgcaaatt gccgtgtcct tcaatccatt ttgtccaaag tcatcaagca
     3061 acaaccgagc ctcaaagtca ttgtccacca gtgggctttt gttaaccctg aggagtctgc
     3121 acgtgtggca aaacagctcc aagcagccta cactcttgat ccaaaagctt atgggaagct
     3181 gaatgaggct ttccttgcgc ttaaggatgt tccgaatcaa aaaacgatgg atgctttgat
     3241 tgcaggcgca aaatatgatg ttaaaaaagt caatgaactt gccgcaagcg aggatgcaac
     3301 aaaggctatt gatgccacac gcactcttgc gaaggaactg aaacttccgg gtgcacctat
     3361 tttgatggcc ctaagtcccg aagggcagtt aatgctcatc ccccctgtga gcgaacaaga
     3421 tcttaataaa attgtaagtg atttgaccaa agcgatggca gcggcgggca cttcttcaca
     3481 aaaggccaca gcacctggga caaagtctta agaggcgcgc gttttaaaaa aggggctgtg
     3541 ttgcccctct tttctactca tctgactttt ttttctaaga agtgattaca agattccttt
     3601 tctcgtcagc atgttcagca tactcaccct caaagcttaa gagcctgctt gttccatagt
     3661 atccctcgag atttcccctc ccaaaatcgc ctgcactgca atctttgatc agagaattcc
     3721 tttgcgccag tattcataat cacccacatt gaagtttatt ttttcttgag gaggaagcaa
     3781 ctcatattcc gcagttttat gaggtttatt atcaagagcg gtaattttct cagtgtattt
     3841 ctcgatttct tcaagatgat ttttctgaag cacctcacct taagattgct tagtttctcc
     3901 tcaatgtgct cgccctcacg ggagatatat taagcgctcc cctccccata agcatacaga
     3961 gatgagaggg attagatttt ttcaaaaaga ctgtctgcag agcttaactc acacacaccc
     4021 gcattctctt ccacaaaaag atgcgtcacg ataccgtcgt caatgaccat cacatagcgt
     4081 ttggagcgca cgccaaggcc caaagatgta agatcaagtt ccatgccaag ggcttttgtc
     4141 aaaagtgcac ttccatccgc gaggaaaata atgcgatcat ccgtgatgtc gtgttccttt
     4201 gcccatgcct gcagcacaaa aggatcattc accgctagac aaaaaacata atcaacattc
     4261 tttgccttaa ggtcatcacg ctttcttata tagcttggca gatgctgtat ggaacacgtc
     4321 ggcgtaaaag caccgggaac agcaaaaaga atacagcgcc ccttggcgag ttcttctgta
     4381 ctaaaatcct gaatgacgcc actctcaact tttttaagcg caatggtctt tggaaagtgc
     4441 tgcccaacgg tgatcatgat aacctctttt ccttgtcaga atgttaacta taggcaagat
     4501 accttaggat tttttttccg ctgcttgctc ttgttgctgt ttataaagag ccgcaaaatc
     4561 cacaggggct aaaagaaggg gtgggaaacc tccctcgcgc gtcatgtcag agataatatt
     4621 tcgtgcataa ggaaaaataa ggtggggtgc ttcgatcatg acaaaaggac cgatggcacc
     4681 ttcttcaagg ttttcaaccg caacaatggc tgcatagcta agttctgcta tgaacaaaac
     4741 actatcccca cgtttagctt ccacacgaat gtcaagaacc acttcgaaat tttcatcccc
     4801 cacattatct gcacgtgcgc cgatattcac agcaatatta ggctgaatat ctgtatcttg
     4861 agaaaaagct gcaatagggt cagggttctc aaaggaaaga tctttgacgt attgggccct
     4921 gatcgtgagc ggaagagtaa tgggtttatt ggccataaaa attcttattc ataaaaggtt
     4981 atttcaagga ttctagcaat ttgatcacag agacgtctag aggataatga tggatttgtt
     5041 ataaaggaaa aaggtttaaa aaaattatat atatttttta ttttctcatg tagactaggc
     5101 ccagtttttt ataaagagaa aaagcaaatg cctcgtctca tcaaactgtt cttttttcca
     5161 tgtgcttttt tgatgatgac tccaccatct cctgtctatg ctcaagcaga tccaaaaaaa
     5221 tcttcgcaca tggcggatca aaacacagct caagaatcac agagttccta taaagtttcc
     5281 ttcgatggat tctcaaagaa ctttccaaag gtacgccctt cgagtggagc acctgtggaa
     5341 attgtatatg ttaattttag agacgatcac gagcttgtca ttgcggcagc tcatgctatc
     5401 gctaaaaaaa ttcccttggg tacagagctt attgtgattt tgggggacca agcaaatggt
     5461 cttggtgtat tagtcgcaac acaagcgaaa cttccatgga ttattataaa aaacaaggag
     5521 ggcattgagg gagtttctaa aaaaatagcg tatagttcga ttacatctgg cagcaaaaaa
     5581 atgtttttgt ctcctctcca attggaagcc atcaaaggca aaaaagttgt cattatggac
     5641 gatgtcatct ccacaggcag cacagtcaaa gcagcggcaa gccttattgg ggatagtgtt
     5701 caaaagattt tagggtttat gtgtgtcttt acagaggagg tgcaacatac agaaattaat
     5761 ggtattcctg tgactagtct tggacatttg cctgtttttc cgttaaaaag agcttaattg
     5821 tttttgatca ggtgctgagc cctttaaagt ttatgttttt gcacctatat cgttttttat
     5881 cttaccttgc cgctcctttt ttatattttt atttaaaaaa acgcgcacac cttggcaaag
     5941 aagacattca tcgtcttcat gaacgccggg gcatcccctc acttaagcgc ccagaaggta
     6001 aacttatctg gcttcatggt gtcagtgtcg gtgaaacgct atcactactt tccttgatcc
     6061 agcacctctc aaaaacgtat ccccaagcgt atattttatt aacaacagga accgtcacag
     6121 cagcaaatat catcaaagat aaactaccgc cccatgcgat tcatcaatac atccccttgg
     6181 atgtttaccc gtggatgcaa cgttttttaa actactggaa acctgatgtt gttggcatta
     6241 cagaatccga gatttggccc aatatgattt cattgtgcaa aaaaaaatct attccccttt
     6301 atctgattaa tgcgcgcctt acagaacata gttttcaaag atggcaacgc atacctacac
     6361 ttgcacgtgc actgttcagc agctttacaa gtatttttgc tcagtcagaa aaaaatgcag
     6421 agcgcctgag atgcttcacg gataaaggtg tccacgtcat gcccaatctc aaatttgcgg
     6481 caaacccttt gacctatagc gaggaagatt ttaaaatatt gtctgcagtt atcaaaaatc
     6541 gcccaacatt tgttgcggcc agcacccatg agggagagga gactattatt cttgaaagtt
     6601 tcaaagatat cctcaaatcc caccccaatg ttttgctttt tctcgtgccc cgccaccccc
     6661 atcgcagtca tcctattata aagcttatcg atagctatgg tttttcctat gcacaacgca
     6721 gcaaagaaga gatgcctaac acacatcata gcattta
//
LOCUS       DAIAHG010000055         6498 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_55_length_6498_cov_3.407569, whole genome shotgun sequence.
ACCESSION   DAIAHG010000055 DAIAHG010000000
VERSION     DAIAHG010000055.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55_length_6498_cov_3.407569"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1950 a         1215 c         1439 g         1894 t
ORIGIN      
        1 agtggagaaa cattttgatt aaggatcaaa ctattattga ttaattttag gtactcaata
       61 tgatgcttat gatctgttga aaaagtgact ttgacccgtt cgctgtgaaa gccttctatg
      121 gttaagtgtt ctccttttgc atgacacgtt gtccatttta aaaaagggat acgtatataa
      181 atagcaaggg ctgatacttt tttataagtg gctttgccga taaaaatata aaaaccacgc
      241 gtgttaaggc gaattttttt ctcatcaagg acaataccat cggtgttttc aatttgttgg
      301 agaagccata catgttttcc gtaatcatat ccaagagcaa gaaccacgca taaaacaatt
      361 ccctgaacaa agcgcttaat cattttaaat tctccaaaag tttaaaagac tccgtttcaa
      421 tggcattgtg aagatgtatc ataaaatcct ttttgctgag gcccggcaaa atggggggaa
      481 ggatttttat ggtggccaca ccgggctttc tgaaaaatcc gcgcttaccc caaaaaatgc
      541 ctgtattcac agcaataggg gcgactggaa tttttaatgc atcatacatc acagcaatgc
      601 cgttttgata tttgacctct gcgccggggc gtgtgcgcgt accttctgga aaaatgagaa
      661 gagggcgaca ggcgcctgct ttttcttttt cagcagccgt tagaattgtt ttcatcgctt
      721 cttttggctt agagcgatcg atcataatcg catcatattt ccacaataac catccccaaa
      781 aaggaatata gcgcagctct tttttgagaa taataatcgg gtgatcaaaa agaagcggat
      841 acacacatgt ttcaaacgcc gattgatgtt ttgatgcaac gatataaggc cccttgggaa
      901 gattttcaag tccttcaatc ttatagctaa gcccttgaac atatttgagc agaaaaaaga
      961 cagcccgcag ccatgtgcgt gccacaaaca caagagctga ttttggaaga atatgacaga
     1021 aaagactgac aatacaaaaa acagctgttg gaaaaagata gagaatctga aaaagaaaag
     1081 acctgataat gatagataaa gtcggcataa aatggcgcaa gggcttgaaa aatcactttt
     1141 tcttgagact agcacaaaac aaactgctca ggcgaggaag attcatcata tgttctcaac
     1201 tatgaacagg gtgcgcttat atcctaatcc atttcttttt gtagaatgtt agccgcacca
     1261 tctcccaaaa atcctccgat ctgggcctcc caaagggttt tgtaaagtcc tccttggttt
     1321 agtaactcat gatgcgtgcc atcttcgaca atccgtccct tgtcaaaaac aagaatacga
     1381 tccatgtgca acaatgtcga cagacggtgg gcaatgacaa gggttgtttt tttgttcata
     1441 aggtccaaga gtgactcttg aatcacagat tctgtcaccg aatcaagttg ggatgtggct
     1501 tcatcaagga tgagaatggg cgcgtccttc aaaaaagccc gtgcaatggc aatacgttga
     1561 cgttggccgc ccgaaagctt cacacctctt tcgcccacaa gagaatgata tttttgagga
     1621 aggtgaataa taaaatcatg ggcgtgagcc ttttgagcgg cctttatcac gtctgcttct
     1681 gtggatgttt tatcgccgta gtgaatattg tcaaaaagac tgcggtggaa aagagcgggg
     1741 tcttggggga taaggccaat agagcggtgg agcgagtctt gagttacatc agcaatattt
     1801 tgaccatcga taagaatctc tcccttttga gtatcataaa gtcttagaat gagattgata
     1861 aatgtcgttt ttccgccccc tgaatagccg actaaaccga ctttttcgcc tgctttaatc
     1921 gtgacagaga gatcattaaa aagaggattt tgtggagcat agtgaaaata aacattttta
     1981 aaaacgatat cccccttgga gaggttgagc gcttttgcac cttctttatc cttcacatca
     2041 atgggggata gaataatttt aagtccctgt gtgagatcac caaggataag cgtgaatttc
     2101 ccaacctctt taaacaactc ccacaaatga gtaatcatcg ataaattgag ggtaagcacc
     2161 aacgcaaagt cgccggctgt gatagaacca ttcttaaatc cttgcacaag ggtataaagt
     2221 gaaaagcttt gatagagaag aaaagatgtt ccttgaactg tataaacttt gaaaaaatac
     2281 cactcccggg cctgatcagc cttgaccgct ttatccataa tcgtgctgaa atggtgtgct
     2341 tcggcttttt tttgggcgaa aaggcgtacg gacatgatat tcccaataat atcgacaatt
     2401 tgccccatat aggatgatcg tacttcagcg gcaatatgac acacgccgcg tgcttgatag
     2461 gaaaaataga tcgacatcgt gataaaaaaa agactccata ccatcagtgc aagggcaaaa
     2521 gtgctacttg ttgtccaaag ggttagcata gcaatgggca agagaataat gcgcggtaaa
     2581 aaagccttca tcaaaacgtc aatagcatca gggacagagg acatcacatc cttaatcttt
     2641 gcgccaaggc ttcctgcaaa gtgatcttga tacagagtgt gagagtgcaa aaacatacgg
     2701 tccatcaggt attgccccac atgttttttc aaaggagtat taaatttgag ccaaatgtaa
     2761 tcattccagc gccacacaaa tgtaatgatt aagatcgtgg caaaataaaa cagcgctggc
     2821 gttgtgatca tgctgatatc gccctcggga tggacctctc ttacaccatt caccatgagt
     2881 ttaataagat agggttgaag cgacgtctcg atggagatcg ccagtgccac gccaagcata
     2941 ccaaaaatcc attttgaaaa gggtttaagg caatgcaaga caaaagcaag aggtgtggtg
     3001 ggcggagcct ggcgttccat gatatcttct ttaaggaggt aagggatgga ggaaaattct
     3061 agcattaaaa taaaaagatg aaaacccctc agaggtcttt attctaaggg gttttgtttt
     3121 ttagttattt tctttgggtt ccgttattgt tttttctttt ttcttttctt tttcatcatt
     3181 attttccttc tcatcatttt ctttttctaa agggattttt gcattttgag tacgtttaat
     3241 attttctttg agtctctctc catccccaaa gtcgagatgt ggtggtaaat ttccacccca
     3301 tccgtaggag catgcgtgag agatagtaag gttgccacac agaagggttg caacagtcaa
     3361 aaattgtttt atatgtaata gtttcattat gaattctcct aattttgaat tgttaatcat
     3421 ttaaatcatt attttgccgt gtaataaggg caccagacgc cgttgatcgc ttctgaggtc
     3481 aatgcaaagg cttccagatc cacatctctt gtgggtcgga tggacgatgc gccatcatga
     3541 tccaccataa gaaagccttg ccagggcata acggatgcat tttcagtcaa tttaatcgct
     3601 actttttccg ctgtgttctc gtcataggag gacagaaaaa tgctatgatt ttttggggtg
     3661 atgtgggttt ttatataggt ttcaaaacga agattttgtg tgatagcttt ttttatcatc
     3721 gtctcaagac cgtagtgttc gatgagatgt tgtccttttt tatgatcgag cgccgctgca
     3781 tagcttttta atatctctgg attattgaga gctttttcaa aggctttttc acaaacgctc
     3841 atctgatcga tcataaggcg cagattttct tttgaaccac gcccattgtt gagatggtgg
     3901 gatgtgagaa tcctcatggt gggaatatcg cttgcaatct ggcaaagagc gcgctcgtag
     3961 tcggcaacat gctgaccttg aatgcctaaa acatctgctg tggcatatcc agagcaaata
     4021 agggtgagcc gcgccccctc gtaataggtt tgaccaatac gggtgaggag tttattgagg
     4081 taaacaaaag cactacgctc tgccatgtcg gggaggggac ttgtcgtttg atccttagcg
     4141 tttgatttga aaggaaatcc tataagaaaa aattcaatgc gctccccgtt tttaacaaag
     4201 tggcgaattt tttgctcaag attttgaaaa gattccgttt tataaagaga gcccagcata
     4261 tacggttcac atagtgcaag aatcgcttgt gtaactttat gaacttggtg ggtgcttcga
     4321 atgatttttt tggggaagtg aagtgctcga gggtgaacag ctttaagggg agatggtggg
     4381 aaaaatcctg aataggagga tgttttagtc gtaagctttt gagtattgat ctgaactttc
     4441 ataataagta ttcctgcatt tggcgtcgat agaaggggtg tatttttgat aaaatgggtg
     4501 tgtacgatgc caaaagcgaa gtcgcgcgat aatacgattc atcatgatga atgctcctta
     4561 aaatgttaaa ttataatgaa attgatcctt agacaaattc attctctctt gaaaatggac
     4621 aatatgtcaa caagaatttt ttaaaaagtc gaaggggtat gttttttgag taacatatag
     4681 gcttcattaa tttttttaat tttttccaca gatgctgcgt tgttatgatt cgcatcgggg
     4741 tgatgttttt tagccataga gcggaaatgt tttttgagct cttgttttgt ataagggtat
     4801 gaaagctcta aggtcacaag cgcatggtgt atttcttttg aaatttgggg gtggcgttct
     4861 gaagatttct cgcgagatgt atgcccttga aaaggagaag ataaaaaatc atcaaggacg
     4921 ttattgattt ttgtataagc cgtttgcata tgaagcggcc agctgggccg ctgccaagtc
     4981 gtatcttggc gaatataccc ctcaagctct gtatcgggaa tatcctgata ataattccac
     5041 gcggcattgt aagcacgcac atgatccaga caaaaccaat aaaaatctcc ctcacggttc
     5101 ttaggtgcct taaacgcacc aaggtttgga caattttcat ggtcgcaata atcaccctcc
     5161 cggttgagag agtgtttagg accatcaaat atatgtgagt aaggatgtgg cattctttca
     5221 gaataacata aaaagaaggc cttgtggggg gaataataga gggtgaaagg atgtttttgc
     5281 taagctgcgc gataccattt atgtgtttca gagcgcggaa gtgcatccac gccatgggct
     5341 tcgataaagg cgacgacggc ccaccgtgca aaaggttttc caatgtgcga aagatcctgt
     5401 tggataacat catgccatcc tggatgaaag ggacttgtct tgccaagaac tttttcataa
     5461 ttactggcga tcataaaaag agcgttgttt tcaatcggct caacacattt aagtgtttga
     5521 gggtctttgc cgatgcgaag acccgtacag ctttcggcaa gggccagcgt aaaggatcct
     5581 gcatcgaaat gaggctttgc aagatattca ccggattctt gccaatcata ttttaaaaag
     5641 cgcactaaaa tatgaacatt ctttgtttca aaaattcttg cagaggcccc tggatagtag
     5701 ggctcaaagg ttcccaaaat actttttgtc acatcataaa cagcattcca aataaccttg
     5761 gcctggtgca taaaatcaag aacaataggg tgttcctgca aaaagtcgtt atattcctca
     5821 aaaatagcgg ggtgaaagtg gaaaaattcc ttgctgtcgt tatacatatg atcctcggga
     5881 ttgcgccgtt gaaagccgat atcaccacgg cgatgctggg gcgctatgga aaaatgcata
     5941 tgtgatttga ttgattcggg cgcgtttaaa aagcgaaaaa aagaggcgac agccgcctca
     6001 atcgtatctt ttgaaagaga aaaaggcaca tgggcatagt atttttcctg cagttggtgt
     6061 gaaagatcat caaaacgggg caaaagcatg agcaaaggtc tccaaaagtg cacatatgtt
     6121 ttttgtatca tacaaaaagg cagctttgga tgaatttttg cagagaggcg caaatcgctt
     6181 agtaaaaaaa tccccccagc ttaaaaaaaa gtggggggag gagatcttga gtagaggttt
     6241 agttaaaatt aaagaatgaa tttgaatttt gagtaaaaac ttctcctata catataccat
     6301 ctaattctta aatagaagtt aattttaaag attttgaagg aattatggat gtcgatatgt
     6361 tggtggatta tcaagatata ttaaaaaaaa ttttaggaaa aattttccta ggcttcccta
     6421 aaattaacgt tctgttaatt tttccctcgc catactggca tcagaaagaa aaagcatgaa
     6481 acagttatgg cacaccaa
//
LOCUS       DAIAHG010000056         6405 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_56_length_6405_cov_3.493679, whole genome shotgun sequence.
ACCESSION   DAIAHG010000056 DAIAHG010000000
VERSION     DAIAHG010000056.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56_length_6405_cov_3.493679"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1910 a         1401 c         1227 g         1867 t
ORIGIN      
        1 gccacctagg gtggtagggt gggggtgcga aaattaactt tttgttaagg tgtgtgaata
       61 aaaggttaat gttttaaaaa aaatctttcg ccctttgaag aaaatttaag gtcccttgcc
      121 ctttaacatg ggcctttaac gcagcgatca gcgcgccttt ctcgccgaaa aagaccgttt
      181 caacgcataa aatcgtcaaa aaagccctca taacccccaa aaatggcgtt atatccagga
      241 aaattcgcct taaaatccta aaaattcacc tattatcacg cctaatgcgt taaaaaagct
      301 attttttgcc cgaaaaacac acaaaatctc gaccaagaaa gcgaaaaatt attaatcacg
      361 ctacttttat gaaaaaaaaa gttcgcccca aatgtacgct caaatctaaa aacacaccca
      421 atacgtcaaa aaattggaat aaatcactaa aaaaatgcac ctataactca atataagtgc
      481 attaaatttt ctgacttagt gaaaaaactt aagcgttttc actttgtgcc attgcctctt
      541 ccatggaaag tgccacatta accttgatca tggcgtgcac ctcgggatga agaacgatct
      601 ttgcctcatg aattcccaat gatttaatgg gtttttcgat gagaacttgg gtgcgaacaa
      661 cagtgatctt ttgctccaaa agaccatcga caatatcttt tggacgaatc gaaccataga
      721 gatgtcctgt ctcaccggcc tgacgaatga gcgttacaac aacgccttca atcgcctttg
      781 acgcatgatg ggcttcgtcg cgacgcttaa tattttgcgc ctcaagatgg gctttttgcg
      841 cttcaaagtg ggctaaattt gcttttgtcg cacggagtgc ttttttttgt ggcaacagat
      901 agttacgcgc ataacccgct tttactgtaa caagttgccc catatggcca agcttttcca
      961 cacgttctaa taaaataata tccataatat ctttccctct tatttgctaa cgtatggcat
     1021 caatgctaaa aagcgtgcac gcttaatcgc cgtagagagc tctcgttgtt tttttgtaga
     1081 gacagaactg atgcggcaag gcgttacttt tccacgctca gatacatagc gagagagcat
     1141 acgcacatct ttataatcaa tcttttgtgc atttttacca ctaaaagggc agcttttttt
     1201 acgcttaaaa aactgacgct tcacaagctg ggccacacgg gcttgacctt catccatcga
     1261 gctgctgcgt gattctgaat ctcttctttg ataagccatg attattctcc tgctgtttct
     1321 acggtgttat tggaagaatt ttcttctgca acagcaacaa cttccgtttg gtcttcatca
     1381 tacgatgaac gtgctttttc atcgcggaaa ctacgttgtt gcatcaatgc ggatggatta
     1441 tcatccaaag actcaacacg cacagtcaga tagcggagaa gatcctcaga aagcttcatc
     1501 tgacgctcga tttctgcaat cgttgcaggc gccgctgtga cattcataag gacatagtgc
     1561 ccttttttat ttttcttaat aggatacgca agggtgcgaa gaccacaaaa ctcgatcttt
     1621 cccacttgac caccgtcatt tttaatcact gacgtatagt gctttgccat attttcaact
     1681 tgcgcagacg taatatcttg tcgcgcaaca aaaatgcatt cataaaatcg cataatattc
     1741 ctttcggttg tttatcaaag gtaaaacctc gacacagccc tttttaaaaa aaagagcaag
     1801 gaacgtcggt ttaaacagag ccaatactag gcatttttat aaaaaaacgc aagagaaaat
     1861 attgaaaatc aatcaccgat aaactacata agaaatacat ttgaattatt tttttatatt
     1921 atcaatatga actttatttt attatttttt atacttttta attcttttgt ttttgcctct
     1981 gatttctccg atgatgatga gaagaaaata ggatacagct attacacaaa agaaaaaata
     2041 gaaaacattg agctttttat agagaagtgc accatataca actttgacac gcatacagtt
     2101 ttaaagtggg ccggtgattt caggcactcc gaggataaat attttcagag tcttgcaaag
     2161 aaaattcttt ggaaaatcct cgacacaaag gatttagacc tcgaagccta ctcaaaagcc
     2221 taccttagcc tcgcaaaaat tactcaaaaa gaaggcaacc cgtttgaggc gattgagctc
     2281 ttgaaaaagt cagacaaact aaatcccaat tatgccgcaa cccaagagtt acttggtcag
     2341 ttttatgcgc acctttcaat caattgtgat tggacagatg acgctataaa gtggaacaaa
     2401 aaagccataa aaacagccaa acatagtctt aaagacatca aagcaaatac gttttggccc
     2461 ctcccccttg aaaaagagca cgaggtttat gcaaaaaacc ttgagaataa aaaagtgatc
     2521 acacggtctt accttggcct tggaggcatt tattctcaga aaaaaaatga tacaaaatcc
     2581 cttaagtact ataaaaaagc aggaaaaaaa tcatcaaaaa tgctcgaaaa atataaaaat
     2641 cttcacaaga aaaatggttt taaatgcaat attccagaga tagaaaatct taaagaaaaa
     2701 tccgagaaag gcattgaaaa aacactgcat tttatagaaa aaaagaaaaa aaattaacct
     2761 acactcctct gccctctgct attttcttct atttcttcat tgtgaaaaaa acacagatta
     2821 tggtattcta aaacaaggtg ctgcgctgtt ttgtgagaca tgcaatccct cggcccatga
     2881 gttctcatcg ggagctgtcc ctgtccttag ctgtggcttt ggatacatgg cccccaccta
     2941 gcgtttctgg ctagagagga cgcaaagatc cacggcatgc gcggcaccac cctttgacaa
     3001 agtttttaac atttgagaaa ggcaccttgg tgctgagcgc attcacaaga cttccccctc
     3061 ccctcctttt tctcatagga ttgtctcttt ttcttataca gcttttgttt atgtggccag
     3121 gcggcatgac acccgatagc cacgttcaat acgccatggc ggtttctggt cagtatagtg
     3181 atcatcatcc ctttattatg tcttttttat ggcgtcagct cgattacctt tataaaggac
     3241 cggggctgat gctaaccttt cacctcagcc ttttgtgggg gagtgttttt tatctttaca
     3301 aaagtcttag accctatcct tggcgcccct tagtcctttt gattcctttt attccaaata
     3361 tatgggtcta tgaaaatatg atttggaagg acgtggggtg tgcatttgct tatcttggcg
     3421 tcctttcctt tatggcttac gcctgccttc agaaaataaa aataaatatt tttgcacttt
     3481 tgattctttt gcttttgctc acctatggca cccttgtgaa gtttcaagca gcatttttag
     3541 cgccgattgc ccttgtatgg atcgcgtggc acgcggctga ttaccaaggg ctcaagcggt
     3601 ttgttttccc atttatcgcc atcgccgctg ttttttacag tatcctctca ggcgtcttgt
     3661 ggatgggacc taaggtcacc cctgatcact cctggcagtt tgttaagctt tatgatctgg
     3721 ctgccattag tgtgggaacg aatgaaaatc tttttccaaa ggcgaatgaa acaaaccttt
     3781 tttcaatgaa agaactccat cagcggttca atcatcagcg cgtcgacgat atgattttta
     3841 caaaccctat tttgcaaaaa ggaaagaccg acgacgaacg cagcgccctt tacaatacgt
     3901 ggctgcatca ggttttaaaa cacccgttta tttattttcg ccatcgctta agcaatcttg
     3961 cttacattgt gctctctatt cctgattttg agcatatcac gtcctttatc catcagcata
     4021 cggagggcaa ccctcttttg gaaaaaaatc tgatacgtat agcgcgcatt ccggggactc
     4081 tttttcttgc gcatatcgct acactttttt taggagttat ttatttttgt ctctcagtca
     4141 taagcttttt aaaaaggaga gaggaaatag cggctatccc tttatttttt atgaatacga
     4201 ttgccttagc acttgtgagc gttttattat tttgctctat ggcaggcacg ccacgttaca
     4261 cttacctgag tattgttctg atccacgcct cccacggttt tgcgtatatc tgctggcgca
     4321 ctctccgtca gaaggtttca cgatcataag cgatgcaaca cattgtcccc cgcccctttc
     4381 aatggataaa acgcttttta cccaggagcc ttttggggcg ttttacaatc attcttttag
     4441 ttcccctgat tcttgttcaa attgttctga gctatatttt tttcgatcgt catacagaca
     4501 ctattattcg ccttctttcg cacacgattg cgggtgatat tgcccttgta agccatatgg
     4561 tagaagatgg aaagtcaatc aaacaagtat ccgctctcgc ctcaaaacac ctaagcctca
     4621 gcattcattt tgacaaaaaa gcaattctta aagaaacggg gcaggcgaaa gaaacatggc
     4681 tttatgaaca tcttaatcat gctctttccg agcagcttaa tcacccttat ttttttcgaa
     4741 tggacaaaga ctatatttat gtggatgtgc agctaagtga tggcgttttg aaagtggaaa
     4801 cactgcgcaa acgtcttttt agcaaaacaa cataccttgt cttaatttgg acaacagcct
     4861 ctgcagtctt gctttttctc gtggctattc tttttatgcg caatcaaatt aaacctattc
     4921 gtaaactagc agatgcggca gagcgctttg gaaaggggca tgagattatt gcctttaaac
     4981 cagaaggagc ccttgaggtg cgcaaagctg caaaagcctt taatcaaatg cgtcttcgta
     5041 ttttaagatt cgtcgaggaa cgcacacagc aacttgccgg tgtctcccat gatctgcgca
     5101 cacctcttac acgcatgaaa ctccggcttg cacttatgcc cgcctcagaa gaccaacaaa
     5161 atcttcaaga agatgtagat caaatgacac aaatgacaca agggttttta gattttgccc
     5221 gtggtgctat ggaagagccc tcggtggagg taaacctcaa agattttatt gaggagatcg
     5281 ccaatgactt tcaacaccat tatccacaat ttcttacaat aaaatcactt tttgatcaaa
     5341 aaataatgat aaaaccccaa cttcttaacc gttgcttaac gaatgtcctg ttaaactctc
     5401 agaagtacgc caaagaagca cagctcagta tgcgcctcaa ggggcacttt attgagattg
     5461 atgtcgatga tgatgggcct gggattcccc ttgaaaaaag gaatgccgtt ttcaagcctt
     5521 tcatacggtt agacgaagcg cgcaattccg aaacaggggg tgtggggctt ggtcttacca
     5581 ttgtcagaga cgctatacac catcacggtg gtcgtgttac acttgacaca tcacctcttg
     5641 gggggcttcg tgtgcgttta aggataccac tttaagggtg ggggatgaac tgtaacaaag
     5701 gagtattatc aatggctgga cgacaaaatc cttttgaatt tttaacatct gcaatgaaaa
     5761 atatgggaat ggaccgtgat actatgattg aaaatcaacg caaaaacatc gaagcattta
     5821 atgacatgag caaaaaagct atggaagtga tgcgctctgt ttctcaaatg caacagcaat
     5881 atattaaaca atcctttgac aatcttgcgt cgatgatgaa ggatgctatg gccggtggca
     5941 tgacgcagga aaccttcaag aaaaatgcag aaagcctcaa aaatcatatg actcaaagcg
     6001 ttgatcatgg catgactgtt gcgagcaaac taagtgaatc tcacaaggaa ttgtatgaaa
     6061 aaatgaaggg tcaagtgact gaacacatga aaaaagctgc tgagaaaaaa tcgtcaaaac
     6121 atcactaatc atcgcttgaa aagtcgtgca gcgcccactc ctttgatatc ccaggaatta
     6181 accaaaattc ctggggtcct ccatggtttt tttacacgcc aaggtggcgt gagcgaggga
     6241 tattatgctt ctcttaactg caaaagcaca gaaaacgggg acgtaaaatc cgctgttctt
     6301 gaaaatcgcc aacgcgttct aacatatttt aaacaccacc cctctcattc caataacagc
     6361 tatcactggg tgggtttgga tcaaaagcat acatctgtcg ttatg
//
LOCUS       DAIAHG010000057         6369 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_57_length_6369_cov_3.882867, whole genome shotgun sequence.
ACCESSION   DAIAHG010000057 DAIAHG010000000
VERSION     DAIAHG010000057.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57_length_6369_cov_3.882867"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2004 a         1465 c         1154 g         1746 t
ORIGIN      
        1 tgtgcataat gcgttaaaaa agctattttt tgcccgaaaa acagagaaaa tagcaaaaaa
       61 aataggattt tccaaggaaa atctaggggc gcgctgataa gagaaatttt taaacaaaac
      121 cttcgcaaaa acgcaactta ccccctcttt ttcatcgatt tttgtgaaaa attcaaaaaa
      181 taaacataaa aatcctgaaa aacacagaaa atcttgtcca gaaagcactc tttccatcga
      241 aaattcacct caaaatctct tttttcactg aaaaccccct ttttttagca aaaaatacga
      301 tggatcttat cgaaattttc aaaatactgg gtcaaaaagc cttttttaca aaatatttca
      361 tgaaaaaccc tagtttcata cgactgttaa gaagagaaaa tttttctaaa aatcgcgtac
      421 aatctccgta tcgccacccc ttaaggagac cagaaccctt gaattcagct ccctccagca
      481 caattatttt tgcgaaaatc gcctcaaaaa tcgcctaacc acgcaaaact catccgtatc
      541 atcgaaaatt ttttcttaaa tccccacgct ctttttaatc tgggtcatat gttgacctgc
      601 aagatcattg gcatgagcgg cgcccttttc taaaacgtga tcaaggtaga ataaatcatg
      661 catataatgc ttaattttct ctcccatcgg caacagagtt tcatggagca cctcaatcag
      721 cgctttttta aggactgaaa atgttttgcc ttcgaatgct ttacagaccg catcaggttt
      781 tttatgcgtt aatgccgcca aaatattcac aagattacgc gcttctggac gattttccat
      841 caatgccatt gaggccgcga tcacgtccgt atccgtttta gcttttttaa tttttcgcgc
      901 aagcgtatcc gcatcatccg tcaaatgaat ccgtgaatag tctgacacat ccgatttact
      961 catcttggac gtgccatcac gcaaactcat aatacgcgtt gcaggtcctg taataacagg
     1021 ctcgggcaga tgaaaaaaat cctttgcaaa acgacggttg aacatccctg ccacatcacg
     1081 ggccagctcc acatgttgct tttgatcttc acctacgggc acatgggttg ccttatacag
     1141 caaaatgtcc gccgccatca aaacaggata ggcataaaga ccaaggcata cactctcgat
     1201 gtttttaccc gctttttctt taaactgtgt catacggttc aaccagccca tgggcgtaat
     1261 acaggaaaaa atccacgcaa gctccgtgtg cgcgggcaca tcactttggc gaaaaataaa
     1321 acatttttca ggagacaaac cacaggctaa ataggttgcc gccataagat aggaattgtg
     1381 ccgcagattt tctgagtcct cgagtgtggt caacgcatgt agatccacaa tgcaaaaaag
     1441 attgtcctgg tttttacttg cctcaagccc atccacccac tgtttaattg cgcctaaata
     1501 attaccaaga tgaataatgc ccgtagggcg cacaccggat aaaacccgtt tcatactttt
     1561 ttcctttgac gacgtataaa tttaaaaaag gtactgagtc caaaaaagtg cgagatcatc
     1621 atataaatca caagggccgc accgataaat cctaaaacac tcataatctc gccaaataaa
     1681 gattcaggcg cacgtataag gctttgaccc cctaaaagcg caaaagccat aagtgtgcaa
     1741 gccagcgtga ccttagcaaa aaagacaaga agacctttat tataaagatt ataccaccct
     1801 ctctttttca tcacataggc aaggattccc gcattcgccc acgcggcaag ccctgttgaa
     1861 agcgcaagac caatatgttc cagcttccaa atcaaaagaa aattaagcgc taaattcaca
     1921 ctaacagaga ctaatgcaac tttgacgggc gttttcgtat cctggcgcgc aaaaaaacag
     1981 gtcgacagta cttttcccac aacataggca ggcaaaccca cagcaaaggc cgcaagtgtg
     2041 ggcgctgtcg cctgaatagc cgcctcatca aattttccat ggccataaat cagcccaata
     2101 atcgggtacg caagggccac aagccccacc gtcgcaggca ttgtgagttg taacgccatc
     2161 tcaatcgcct gattggtcgt atgccgtgca ctcgtatcct cacccaggcg aatttgtttc
     2221 gacaaaagag gcaacagtgc tgtactaatc gccacaccaa aaatagaaag cggcagctgg
     2281 ttgagcctat ccgcataata aagatacgca agagctcctg taggcaaaaa agacgccagc
     2341 atcagatcaa taaaaatatt cacctgcatc acaccggcac caatcgttcc gggccccatg
     2401 agttttaata tttttttgac ctttggcgta atttttggaa aatgcaagcg aagtgcaaaa
     2461 tcgtagcgcg tacatacgat ataaagccac gcgaattgta caatccctgc gataaaaaca
     2521 gccgtgcaga ggctataccc tggatcaaaa ccaaactctc ccgccagtaa aagcgacgaa
     2581 atcataataa tattcagcaa aatcggcgct gcagctgcac tggcaaattt atgaatggaa
     2641 ttgagaattc cccctaaaag cgctgtcagg gaaataaaaa gaatataggg aaaggtaatg
     2701 cgaataaaat taaccgcaag atccatgcgc tctggtgttg tggcaaatcc aggtgcaaga
     2761 atatgaatga tagagggcgt tgcgatgacg actaaaacac aaaaccctgt aagaaaaaat
     2821 gtcataaacg aaaacacatc catcgcaatt ttttgtgctt ctttttttcc tccctcggca
     2881 agttctcctg caaaaatggg gacaaaagcc gcattaaagg ccccttctgc aaaaaaacga
     2941 cgaaagaaat taggaaactt aaacgccacc acaaacgcat ccgccatgag gcccgcacca
     3001 aggaaatgag acatcaaaat ctcgcgcatc atgccaaaaa tacgcgaaag ccccgtatag
     3061 ccgccaatgg ttgttactgc ttttaaaatg ttcaagggtg ttctatgatc gctgtgaata
     3121 tcgccgcatc ataacataaa atgtgtaacg caacgcaata aatttaacac ccttaaggat
     3181 tcagcgactc ttatgctttc tgttaaaaat cttgtgttgc atcgcggtga gaacctgctt
     3241 catcccaata ttcattttac cctcaaaaag ggacaagggc taatccttcg agggcctaat
     3301 ggatgcggaa aaacgacctt cttacacacc ttggcggggc tgataaaaaa tgactttagc
     3361 cccaacattt tgatagcatc agaaactttt ttcctccccc atgttgatgc tcttcttggg
     3421 cacacaacgg tcgaggaaaa tatgattatt tttgcgcaac tcatgggttt gaccttccat
     3481 ttgtcagagg atcccttcaa gatcaaacct tttttagcgc aaaaagttaa ccgactttct
     3541 catggccaaa ggcgccgcgt gtccctctcc cgtcttcttt tgacacacgc aaaattgtgg
     3601 ctcttagatg aaccggagca aggacttgac acccatttca cgcacatttt atgcaaagtt
     3661 cttgatcatc atcttttgaa tggaggtggg attatccttg cgtctcactt tttgcccact
     3721 caaaagaatt ggcaagaatt aaccttttgc cagctacatg aaaagtctct tgaaaaatca
     3781 tcattttttt gagaggatac tcaaaattat ctatttagcc tcaaaaaaac atgaaatatc
     3841 tcggtctttt tttcaccctt ttggccctcc cccccttaac atcttgctcc accacaaagg
     3901 gaaatgctgt tgtgggcgaa tcctcaaagg acgaagtcta ccgtattatt aacgatcaag
     3961 tttcgacaaa atccgatgca cgtcgcgttt ttagtgatcc aagcgatatc gattttgata
     4021 accaaggcaa tgagaaatgg acctatacac acattacgca aacctccatg gtgcgcaatt
     4081 acattcccgt tgttaacttt ttcacaaaag gcacagacga tacccacaaa aaaattgtgc
     4141 ttatttttga tcccaaaggt acgctcatca aaagtgctgt gagtgaatcc aagggcgaaa
     4201 caaagtggta aaagcataaa acgacttatt ctttgcgggc aggtgagact ttttgcctaa
     4261 gtcgttttat ttttattcat tatctgccct tggcatcaga gaaaaagcgt tgtaaaattt
     4321 ttttataaat ttttttataa attttacatt ttgatatcat cactcctcca cacgcttctg
     4381 gatatttctt ttttccaagc ttttgagctt gctttttttc tatgccttat actatgggcc
     4441 cttttatgga gcttggagtc gaaaacactt caaaaaaaag ttgatcagga aaaatatctt
     4501 cgcggttttt tatgcgaatc attaagcgaa atgaatgtac ccatggcgta tttttcacat
     4561 gatgggtatc cctatatgaa tgaggatttt ttagcaattt ttaatatcca gccccatgaa
     4621 gatctaaagg aagcgattct tcaacgtatt caaaaacaaa tccctgcgga gacatgggag
     4681 gcgtttattc gctatcttat gtttcaatca gaagagctta tgtgtgtgat ggagcaagaa
     4741 ggccaaagct attgtctgca cgcacgcgct tatgatagta ccgcttattt atggatgagc
     4801 cccctcaaaa actcagaaga tgactatctt tatgccaaac gcatcatcaa caatatcccc
     4861 attgcgctgt ggtatcgcaa tcatcacagt gacattgtct attgcaatga tgtctatgct
     4921 aaaattttgg gaaaaacagc cgaggatgtg atctttgaaa aaacagaact ttgcccaccg
     4981 ggacgccttg gcagccccta taagcaaagt caaaaagcca ttacagaaaa aacacctcaa
     5041 acagatcgta tgcatgctgt cattgatggt tctcgaaggc ttttagaagt aggcgccgtt
     5101 cctttctcta ataaaagctc ccttggctac gcgtttgata tcacagaaat agaagatctt
     5161 caaaaaaaac tcagcaaata catagaaagt agtcatgatc ttttggatca tattgcatca
     5221 tcaattgcga tttatggtcc tgatacgcgt cttcattatt ttaatcgtgc ttatgtgaat
     5281 ttatttcagt ttgatgaaaa ttggctaaga tctaatccca ctttaggtga tgttctcgaa
     5341 gatttgcgta cacggcgaag acttccggaa taccctaact ttcaacatta taaaaaaacg
     5401 cgcatgcagt ttttcaataa tctcattgat cctcttcaag atcttgtgca tcaacccgat
     5461 ggcaaaatat tccgcatgct tgtggccccc cacccccttg gcggtatttt ttatattttt
     5521 gatgatgtga gtgaaaaaat aacactcgag cgccaatata ataccctcat tgctgtgcaa
     5581 caggaaacga ttgataatct ttttgatgcc attttagttt ttggatcaga ccatcgtctt
     5641 cagattatga atcgttcttt ttgtaaaatg ttcgatgtga atgaacccct gtggttttca
     5701 ggaaaaacac ttcaagaggt tattgatatt gacaatccta atcttgtcat gacaaaaacc
     5761 gaattactca gcctctttat gaatcttttt aataagcgtc atccgacaca agatcacatg
     5821 gaactcacaa gtgggcgcat tttgcgctat agttatatcc ctcttcccga tggatcacat
     5881 cttatgagtt ttattgatat gacagactct atccgcttcc aagaaacgct taaagagcgc
     5941 aaccgtattc tcgaagaagt caacgagctt cgatcacgct ttattcatga tgtatcttat
     6001 gaaattaaaa caccccttgc ccttatccaa aatcacactg aacagttaca acaagatcag
     6061 cacaatgcac agtcagacca aaagcagctc taccgcacaa aggaaattat tgactccaca
     6121 aaacaccttc tctcgattat taataacatg attgatttat cccacgttga atcaggccaa
     6181 ctaacctgtc ttacaaaaaa agtcaatctt gggaggtttt taaaatccac tgcatcgctt
     6241 gtttccagca aagccaaaga aaaaggcatt gagattatta ttgaaaataa cacagcgatt
     6301 gaaactttta atgcggatga aaagcgcctc aaacaagctt tttttaatat gctaagtggc
     6361 gcactcaga
//
LOCUS       DAIAHG010000058         6333 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_58_length_6333_cov_3.249840, whole genome shotgun sequence.
ACCESSION   DAIAHG010000058 DAIAHG010000000
VERSION     DAIAHG010000058.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58_length_6333_cov_3.249840"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    2690..2699
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1874 a         1262 c         1356 g         1831 t
ORIGIN      
        1 taagcttgtg cgcgatattt gtgacaaagg gctttatctt acatctcttc cgggcccatc
       61 atcggtgatg acagccttga cactttctgg ttttccaacc gatcattttg tttttgcagg
      121 atttgttaat acaaaggatt ttgatgctct taaaatgctg ccctataccc ttattttttt
      181 ctctacggcg aacaaactcc ttggcgattt ggctcgcatg agagagattt ttattggaag
      241 gcaagtggct attgtgcgag agatctctaa aatgtttgag gaagttgtgc gtggaacgtt
      301 tgacgacatt gaggtgtatt acaagtctca tgttttaaaa ggagaaattg ttttattgct
      361 gtcgccaaca acttctacgg atgtgattga ttggcaaaca attgatagga ctattctttt
      421 agaaaaaaag acatcggcca aggatctttc agaagctctt gcggaaattt atagaatccc
      481 taagcgcaca ctttatcaac gaattattga gatgagacga ggtgattaat tataaataga
      541 gtgaggattt aaaaactcaa ttttcttaga aaaattcata agataaggaa aggtcatgga
      601 caatagtgtt ttagaaaaaa gtgctcagcg cgttcaaaaa agtctttctc aaagaggtga
      661 gaattttaaa attaccaagg tgccagaggg agcgagaacg gcgcaagatg cggcgacggc
      721 tcttgggtgt gaaattgctc agattgtcaa gtcgctcatt ttttgtacaa aagaaacacg
      781 tcaacctatc cttgtattga ccagtggaat aaaccgggtg aatgagaaaa ttatttcaga
      841 gcatcttggt gaaacaatca tcaaagcaga tgcagacttt acacgagaag tgacaggcta
      901 tgcgatcgga ggcattcccc ctgtcggcca taaagttata ataaggactt ttattgataa
      961 ggatttgctt cagttcgatg agttatgggc ggcggctggc acacctcacg ctgtctttaa
     1021 tctaaaatct gcagagttac agaaattaac aaatggaaca atgataaacg tgatgtaagt
     1081 gaggcgtatt aagttatcta aagactctct ataaaatcca gataagcttt ttgaagcgtg
     1141 agtgtgatgg gtcctggggt gcctgtgcca atggctttat cattgatacg cacaacgggg
     1201 aggccccaaa catttgaact tgtcaaaaat gcctcctcag cccgatacgc tttatcgagg
     1261 gtgaatgctt tttcaacaac gggaatatta agagtctttg caaggatttg aaaacggctt
     1321 ctcgtaacac cattcagaat atgagaagat tttggatgtg tgtgcatagc gccatcaaag
     1381 acgcaccaaa aatttgccga ggatgcttcg gtaagaacat tatttttgat aaaaactgta
     1441 tcatacacgc ctttgtctaa ggcattttgt tttgcgagaa tagcgggcaa aagattcact
     1501 gttttaatat caggtcgcgc ccaccgctgg tcttcttgtg tgataattgc aactcctttt
     1561 gcgtgtttgt ctaaaaaagc aacggcatca ttgggccgta cagtgattac aaggctgggc
     1621 ttggagcttt ttggaaaggg aaaaagtcgc ggtgtgacac ctcttgagat ttgcacgtac
     1681 accatgccgt gagggtagcg attgcggctt aaaagctctt ggatatgatg ttgaatggtg
     1741 tgagtttttt gagcaagagg cagccgaatc tctcgtgcgg aatatgctaa acgatccaag
     1801 tgtgcctcaa ggtcaataag tttttgatca taaaaactaa tgacctcata cacagcatcc
     1861 gcaaattgaa atcctcgctc ctcaatatgc acggatgcct tgttaaaagg aacaaaacaa
     1921 ccattcacat aagcaacacg cgtcatcaga ggattctctt tttattgcga tactaatgcg
     1981 cttgtctagg cggaaattct ttgatcagca tatccatctc gctttgagtg cagagcccca
     2041 aaagaacagg gcttttaggt ttaatattcg atgcattcca atgggttcgc gttttgacag
     2101 agttcaccat atttttcgtt gtgcccaaaa gcttacagat acggatttct gggatttctg
     2161 gatgatattt gagaatccac gcaattgcat cggggcgatc accgcgcttt gcaacaggcg
     2221 tgtatttacc tttcttcttg cctaaaatac ttgcagcatc cataggaact gtcataacga
     2281 ggcgcgcatt gacatcagcc tcgcagcgct cgatctcgct ttgagtaact tgaccggagg
     2341 taacagggtt catgccaatc attccaatgg ctgactcacc atcggcaatg gcttgaacct
     2401 cgagaggatg gagaccgcaa aaatccgaaa tttgctcaaa actaagacct gtattttcaa
     2461 taagccaaac agctgtggcc tttggcataa gaggcaatgt catattaatc tcatccttgg
     2521 ttttttttaa atgtacctca tacatagcaa tttatagaga ttctggctat gtgtttattg
     2581 aggatagggc ctgtaatttt cgcgaagagg ggggtgtttt gctcaataag actcatgccg
     2641 gttgagccta aagtttgtcc acatacgatt gcgttcccgt tcatactcan nnnnnnnnnt
     2701 tgatcttgat ccaattggag ttttttcatc acctcggggt ttgggaaaac actttcgtca
     2761 tccttaaaat cctgggggag gtatttgtga cttttttctg ttgcagtggc aagcagcgta
     2821 aacaaactta cacgcgcaac aatatcagga cgaagcataa aattaataaa tgtatgggcg
     2881 ttgagtggat tgggcgcatc tttgggaatg accatggagt caatccacag tgttgttccc
     2941 tctttgggaa taatatagcg aagattcttt cccagttttt tagcttccct aatggcaatt
     3001 tgcgtatccc cagaccatgc ttgggcaagg catgtttcac cattcatcag ttcgcccaca
     3061 atgcgtgacg agctaaaacg ccgaatgaag gggcgaatct gcataagatg acgctgggcc
     3121 gtgataagat catctatgct tgagctttta agatctttgc caagatacaa aagtacgaga
     3181 ggatacacat cgatggattc ctcgagcaaa gtcaccccac agtctttaaa atgtttgaca
     3241 atctcaggat taaaaaggag tgcataacta tctaaagggg catgaggcag gcgtgcttta
     3301 atcatatctt cgttaaaagc aatcccaagc gtgccccaaa aatagggaac actatactca
     3361 tttccaggat cgactacacg aatttgttca atgagaattg gttctaaatc actataatta
     3421 gggataagag ccttattaat tttttggtaa atccccgctt taatttgatt gccgagataa
     3481 ggcgatgctg agggaaaaac aacgtcataa cctgagtttc cggcaaagag tttagcgctt
     3541 aaaacatcat tattatcata ataatcaatg acaacacgga tccctgtttc tttataaaag
     3601 tcatcgagaa catccttagg gatcatggaa taccaattat aaacattaac aacgggtgta
     3661 ttctcataac gcatatagaa aaaaagagac gccgctagtg ctatcaaaag gactgtgcgc
     3721 gtaataaagt ttcgcgtttg ttgtgtgatc atggtcgtcc tttctctaac actttttgag
     3781 agtgcttttt gaggcataaa aacagcccaa atagtatcat aggaatacaa agaagttggc
     3841 cgtttgtgag ggggataatc gtttgactgc cctgtgccag gggatcttta taaaattcga
     3901 tcataaagcg catgatgccg taagcgaaaa gaaaaaaccc actaatgcgc ccttgagaat
     3961 aacgcatgtc tgttttgatc cacaaaaagc tcagcaaagt caaaagaaca aacccttcta
     4021 aaaacgcctc atagagttgt gtgggatgac gagggagcgg accagcacca ggaaaaataa
     4081 caccccacgg agcatttgta atattgccat aaagctctgc attcatgaaa tttgcaatac
     4141 gaccaaggcc aaggccaatc ggcacagcca cggcaatatt atccatcaga ctgaaaaaag
     4201 aaattttgtg cgacttacaa aagagaaaag ctgcgactaa aacaccagcg agtcctccat
     4261 ggaaagacat cccccccttc caggtcataa aaatttctaa agggtgatgt agataataaa
     4321 gaggatcata gtaaaaaaga acatgaccaa gacgtccacc aataataatg cccacgatgg
     4381 cggcaccata agcaaatcgc tcaatcaatt cttttgagag tccacttgga aagcgggatt
     4441 gcaaaaactt tgcataagac gtcgcaaaga taatactcac aacataagag agcgcatacc
     4501 aatgaatttt caaaggtcca aggtttaatg ctacaggatc aatattggga aaaggaattg
     4561 ccattttttt atcgtcacgt cttagcgata aggatcctct tttaaaagat aattatggac
     4621 atagtcgtaa atcccttctt cgatggatgt catcggtttt gtataaccgg catcccgaag
     4681 ttttttgatg ggggcgtgtg tgtagtattg atagtgtttt atgagttttt cagggaggtc
     4741 aataaactca attttaggcg taagattgag tgctttgaaa atcccatggg ccatgtcaag
     4801 aaacgtgcgt gcttgtcctg tgccaacatt aaagagtccg caaatactat catgggtatg
     4861 cagccaaatc ataacatcca cagcatcatg tacactcaca aaatcacgca tttgatagcc
     4921 gtctttaatg tcatctttca tggatttaaa aaggtgcgct gttcctgtct cttgaatctg
     4981 cttaagaaaa tggggaatga cactgctttg cccctttttg tggtgctcat tagggccata
     5041 gacattgaaa aactttagcc ccacccactg tggcggtggg ggcgtttctt tggaaaacac
     5101 atgctgaagg acactgcgat caaacagatg tttgctccat ccataaagat tcatgggccg
     5161 taattgcgct aagtagtcag cattttcact gtcctcaaac ccctggctgc cattgccata
     5221 ggttgctgct gaagacgcat agataaggcg cacgcgattg cgcccacacc attcgaaaag
     5281 gtcaagagaa aggcgataat tatttttaag gacaagatca ccatccgtgg catctgtgga
     5341 ggaaatggca cctaagtgaa aaatagtctc aatctggttt ttgtgatttt ctaagaaatg
     5401 cggtaattgt tctggggaaa taaagtcata aaggtctcgc ttggcaaggt tatgccagcg
     5461 gtcatcgcat cctaaaacgt cacaggccac aagcccgcgt gttccgcgtt tttcaagttc
     5521 ggcaataagg ttcgatccga tgaatcctgc ggcgcctgtg acaataatca tttttgagct
     5581 ctttcgtttt aatgtcattt ttctataaca ctgcagcagc cttgactata agtcaagcat
     5641 gggctctcgt gtgttcacta cgactcagag aagtttttat tctgactgat ttttccctga
     5701 ttttatgaga gttttcgctt ttatggaact ttttttgagg ataatgctgg catatttgag
     5761 ttgtctcgcg tgagaagtac gcgttttttg agagattttt tgcttttttg aaagattttt
     5821 gcgtattttt tattctaaat tgttggcaaa aaatgcttta taggatgaaa aaagcgattt
     5881 tttcttttta aaaaactcgg ccgcaaggga ccgagtttca aaataacttt aactgcacag
     5941 cccttttctc aaccttatcc atttccttca actgatttct aacatactgc cgtataactt
     6001 cttcagttgc attgcctact gtttctacga aataactctg agtccataat ttaccacccc
     6061 aaaaatattt cttttttatt tcaggataca ttttgaaaaa cagcctagct gatatacttt
     6121 taacacttgc atccaatctg aaggagattt tttaggctca gactgaacaa ccatgtgaat
     6181 aggatctaat aaaatttcaa gctcaacaat ttcaatatta taaagctttt ttttaccctg
     6241 aaacaccact cttactgaat caatcatggt aatgtcttcg agcacatcgc caacggcttg
     6301 atgaagcgct tcaagctctt cagcaatagg gtc
//
LOCUS       DAIAHG010000059         6329 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_59_length_6329_cov_3.039347, whole genome shotgun sequence.
ACCESSION   DAIAHG010000059 DAIAHG010000000
VERSION     DAIAHG010000059.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6329)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59_length_6329_cov_3.039347"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2164 a         1228 c         1201 g         1736 t
ORIGIN      
        1 agttgagacg aatggggcat ccctatttta gagaataagg agctcactga aaataagatt
       61 tacggttata caaataaagg gcgttaaaga gtatgtcagc acaaaataat gacacaaaac
      121 aacagatcgc agaagaaatc tctgaacaga aattaaaagt atttcagacc cgccggccta
      181 cggtagaagc tttggaagag attctttttg agattattcc ggtattggat catggttttt
      241 tacgcttaat tgattatatg ggaaatgatg cagcaattgt taaagctgca cgggtttctt
      301 atggtcaagg aacgaaaaag acacgggaag atcaagggct cattagctat cttatgcgtc
      361 atcgacattc cactcctttt gaaatgtgtg aaattaagtt tcatgtcaag atgcctattt
      421 ttgtggcacg ccaatggctc cgtcatcgca cagccagcgt aaatgaatat tctgggcgtt
      481 attcgattat ggataaagaa ttttatatcc cagcgccaga acatattgcg gcccaatcac
      541 tatccaacaa tcaaggacgc ggtgatattc ttgaagggcg cgacgctgca cgtgtgctgg
      601 agtacctcaa gcatgacgcg cttttgacct ataaacacta tcaagagctc attaattttg
      661 atgatgaggg caacatcatt gacgaggaca gaccaagcct tgcccgagaa cttgcacgga
      721 tgaatttgcc tgttaacttt tatacacagt tttattggaa aattgatctt cataatttta
      781 tgcacttcct cagcctgcgc gcggatagcc atgctcaata tgaaattcgt gtgtacgcag
      841 aaaaaatgct tgagatcctc aaagcgtgga tgcccatgac gcaccatgcc tttatggagt
      901 ataaaatggg cgcgacccat ctttcaaaaa cagcaacaga tattatcagg cgtcgtctga
      961 aaggtgaagc gatcgatgca aagtcctcgg gccttagcgc acgcgagtgg ggcgagctcg
     1021 tcactctttt tccagaggta ggggcgtaaa atccccctgc ccttaaaaaa tttttccccc
     1081 tgtcgccccc ataaggagat ttttatgcgt aaattaataa aaataataat aagtttaagt
     1141 ttattttttg gaatcatagc cagcatacac aacacaccag tccatgcatc agaagaagga
     1201 aaaaagaatc aggaaatttc ttctgaaaac aaatactata aaagtttaca caaaaccctg
     1261 ataaaacaat ttcgaaagaa actcattaat cttgataatt ctttttcaaa agaggataag
     1321 aaagctttct ataaagttat taatgataaa aaacacaaaa aattagaaga aaaatttata
     1381 aaaataaatg aaaaatacat gaaaaatcct aaaaaggatc agaagaaatt agagaaagag
     1441 catgtttatc aggaatacgt taataatatt ctgaaaaaac acaatattaa tcaaaatgat
     1501 tttgatgaaa ttaattactt gtattatatg acgaaaataa ttgccactac agttatagat
     1561 aatctcataa acccttacgt tgatgaaatc attaaaactt acgccgatac attagatgaa
     1621 aaagatcccc tcaaaatgta tataaaaaat gtctacattg cagatgaaaa aattaaaaaa
     1681 aagaccgagg ttgaagaact ttttaaccag tatacaaacc tgatgaaaaa aacatctccc
     1741 aaaaaaagaa aattagagga ttgggaaaat ataggtaagt taatggtaaa atttcatttt
     1801 acgtatgaca acatctttaa taataaattc ttaataaaaa tttataaaaa acaaggcctt
     1861 acgatttcga caaatgagat tgaaaagctc agtaaaaaat tttggaagat acatctaaaa
     1921 aaagaaaaag agattgttaa taacgaaaag ctttatcttt caattatgaa tgacgtagaa
     1981 aaagaacttg gccttgatta gaattttgtg agggcgcggt gcagtatccc ccgtacgctc
     2041 ttgacacgac agaaccttat gaaataatag tcgtgagtac tctcatacat agaaaagctc
     2101 tatcttatgc aaaaaatata taaaattctc ctgaccttgt tttttgttca atcctcggca
     2161 cagtgtctgg ccagagaata cgaggaaaaa gagctcaata ccgatgagat cattactttc
     2221 gagagtggat ttaaccttaa ggaattaaag ctcaaggaac aacaagaaca atttaaaaac
     2281 attcaaaccc cagaagaatt tgaggtgtat atgaatgatt actttccggg gaaaacaatt
     2341 atcagcaagg cccttattac ggaaacaaaa caagcccttg ggtggcctgt agagaataca
     2401 gataaaacct atgaacggca gcgactgatt gggtatgaat acctcgcctc tgttgttgaa
     2461 aaaaatacat ttctaacact tgtggatact tttcttgggg cgagtcaaaa agatttggaa
     2521 aactattttc gtgaaggtgc taaagaagat gaggtgaata aaattcgctt tgtgatgctt
     2581 gtgatggaat cctacacaaa aaacagtaaa aatgacgaaa ttacacattt ttcatcgcat
     2641 ttgaaggata tttttaaaag tgagcttgag caaaaaaaga aaaaagataa ggagatagaa
     2701 aattttcaac aacgtgggct tttgaaaaac gattcctctt ttaaatgttt tgatttaatt
     2761 ttcacaaaga tgccatcgct tttcatgaaa aattatccaa cctatacaga aaaagaatta
     2821 gagataagcc ttaccctaag ttttattgat tctcttattg ttgaaaaatt aaaaaaagag
     2881 tgaaaccacc tcgcaaacct cctgcaaaaa atacgttatc ctctgatgat aaaaaactgt
     2941 gggaggcctt taaacgcgcc gaggatataa cgcctcttgt gcattcagac aaagtcgtgc
     3001 cgaccctccc ctcgcccctt cctgcttttt atcaaaaacg cgcagcagac tggactgaag
     3061 aacctttttt taagcgccct caacgtgatt ttgacgcgcg cattgatctc catggtttaa
     3121 cagaagacaa agcctatgaa cgcctttgtg tctttttaga aaaaaaacaa cagcaaggtg
     3181 ccaggcgtgt tcttgtgatt acgggaaaaa gcggtatttt atcagaaatt gttccaaaat
     3241 ggcttttagc aatgcccttt gtgatggata ttgagccctc aaaagcgatt catggcggca
     3301 aaggagccct ctatatcacg ttaaaaaaac gcatcatcga ttaacttttt ttttactctt
     3361 ttcccctaac atcatcttaa aaggtagaga tatgttggct ttgatgattt ctttttttct
     3421 tacacttatt ttatccgtta tgcctctgtc aacaagcgac gctggaaatt acgggaaata
     3481 taaatcgtat gataagcatg aaaaaaaaga ggataaagag gaactcaaag aacgctatga
     3541 agaatcttat aaaaaagctc ttttaaagct gcttgagcac ctccatcata tgtccaaaat
     3601 gccttctggc tgttggattt ctaaggaatg ctatggccct tataaagaca tctttgagaa
     3661 tgtttaccgt gactatcccg atcacaaccg ctatcatacg gatcccgtgg acgagcgcat
     3721 ccgtcgccat atcgtcaaat tacgcgacga gagtattcga ttgatcaaag attattcttt
     3781 tcctttttca acgtatcttc ttgggacact tccagaagaa gccgtagaag ctcttgtgga
     3841 gggaaagttt ttcacgctgc caacgcttga agaacttaaa aagctccctg caggtgcgct
     3901 taacgaaaaa gatcttgcga ttctttttta tcaatatgcc cttaaaattc aaaatagcct
     3961 gaaaagtgat ggcgtttatg gcgtaaaaga attcttcaat gctatggaaa aagtgataaa
     4021 tgcccttccc aaaggttatc gccgggaatc ccttgatgac tccttagatc acctaaaaaa
     4081 acttatcagt catcatgatt ttagccgcgc cattgcacgc atgattaccg aggagctcaa
     4141 acgcattgac cctgaagagg cttctcagca aaaactccct gaaacggcca gttacaggac
     4201 tttcaaaaaa cttaaagatc tccaaaaaca gattatggaa gctaaaaaaa cctatacaag
     4261 cgatccgttc aaagaggatg tgcgcgagaa aaaaaaggat gagaccttca aaaaatttta
     4321 tgaaatggtg tataaaaccc ctgactcaaa ggggcatgat gatgaaaaat attggctgcg
     4381 tgttttgctc gaggccttta aaaaacttcc tgaaaataac gcgctgcgtc aatcgatctc
     4441 gtcctctgac tttatcaatt cccttaagga ataccttcat catggccgct atccaaatgt
     4501 aggattctct atctatgaaa atctttcgcc tgagtggcaa gataaaatta aaaacggtga
     4561 acgtttgaac gaaaatgtaa gtgatagtga gcttaaaacg gctattgaaa ataaaaaact
     4621 cacactcacc cacatggccg tgatctgcct tgagaagatt caaaaaatta aaaaagaggg
     4681 ctctgttgaa aaatataaaa aggctgtgca gcacacaatt gaaaaaataa cagatcgttt
     4741 tgataaggtt ttaagatcga ttaacaagga taaaaccaac actgaatcaa caaaactcca
     4801 ccaccttatt gagaatgcct tgaaggagtc agaagaggat atctccatca aaagccttgt
     4861 ccttgagtgg ctctatgaac aaatccatga taaagaggcg cttacgtttg atgctaaaaa
     4921 aggacaaatt gtagaatttt ccgaacagac ctcaaagggg aaaaaacata aaaaacaacc
     4981 tcattggccc catgaacata aaaaagcagc ggaaataatg gcttttgaaa aaatgatggc
     5041 taaacctgtc ggtggacgca cgcactacgc ggataatgat ataatttttg ctggaaatcc
     5101 ctcaaaggat aaggataaaa ataaagaaaa gaaagaacaa aaacctgaaa aagaatttgc
     5161 cactaatctg ctcaaaaaaa cagaaggcct tctcggaggg ttcctcgata atgtttttaa
     5221 gcaagtgggc gcaggtaaat aaacttgaat gatgtgtaag atctgtgcga caaagaaaag
     5281 agagacccac aagagggact gcgaccgatg agtgtagcga caaattattt tcctattttc
     5341 ctttttctca tgatcagttt tggtttatct gtttttttga ttgccctttc ttttttacgc
     5401 agcaaacgca agggatatgc cgaaaaactc tcgccttatg agtgtggatt cgatacgttc
     5461 gacgcccctc caacaaacgc ccgcgccccc ttcgatgcac gtttttacct tgtggccatc
     5521 ctttttatta tttttgacct cgagatcgct tttctttttc cttttgcagt cagcctcccc
     5581 tccattgggc tttttggctt tttatccatg atgttttttc tgtttgtgct taccgttggc
     5641 tttatttatg agtggaaaaa aggtgcactt gattggcact agaataataa aacacaaagg
     5701 acattgagct aaaagcccta gttttaacac agatggctaa gattttttaa tggggagcga
     5761 aaaccaaaaa acgcttccct gccccatttt actctcgcac ccaatttttc ccagaaggag
     5821 tttcactaaa tttttcgcaa tactcaggcc aagcccctgc ccccttgaat ccgcattgag
     5881 cgcctctgct gagaaagaat cactcaaaag tgtctcttga atagcctctg gaatcccaag
     5941 acctgtgtca atgacctgca caatcagctc ataattttta ccctccaagg gggctgtgtt
     6001 cacattaata cgtacctgac cgacttgcgt aaatttaaaa gcattgctcg tcaagtgcaa
     6061 aagaatctgg cgaaaccgca cagcatccgt ttcgaccatc aagggaactg attgactaaa
     6121 aaatgtttca aaacgaaggc ctctttggat agcaacgggc tggaggagac cttgaatttc
     6181 tgagacaatt tcgcgaatat taaccggttg aataaacgcc gtttgctctt ttttctcact
     6241 ttgactaaac tccaaaacat catcgatgag cttcattagt gcattagacg acgcttgaat
     6301 cgtattcaca tacgccgttt gctgttgag
//
LOCUS       DAIAHG010000060         6239 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_60_length_6239_cov_3.276696, whole genome shotgun sequence.
ACCESSION   DAIAHG010000060 DAIAHG010000000
VERSION     DAIAHG010000060.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60_length_6239_cov_3.276696"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1845 a         1365 c         1183 g         1846 t
ORIGIN      
        1 gactacgcca cgcaatgttt ttgtttgctg gcgcgccaaa ataagcgcaa tttgctgtgc
       61 caaaagctca tcatgcaaga ggcgcctata agcctttgat gttggtgtta aaatttcttg
      121 gatattggtt ggatgatgaa caatctcaag tgctttgaga aaacttggat actcataatg
      181 agacgcttgc cattccggcg gtgcactcag attttgcaaa agatagttta taaaatcctg
      241 aattctctga tttgctagac ccgcaatttt aggatagaca atttcttttt gagcgcccaa
      301 ggaagaatct aaaaagtgat ctctgctttt tctcgcccca gaaggatgcg taatgcgata
      361 ggtatcgcca aatttttcaa gtttccccac aatatattta ggacgcccca cagcaaacga
      421 aaacgccaga aactttgtat tagctttttt ataaaaaaag acaagctcga gcacatcttt
      481 tgagggaaga acattgccta taatgcgata ggcccctctt cctttttgaa gagggggaat
      541 gtgttgaaca ggctcgatat caagtaaaat agtttttccg acaagatcag gcctcaagga
      601 tgtacaatga gttttctcaa taattcctgt tggaaaatgt gccaaagcgt caaggactgt
      661 atgaccaaaa agtttttcaa aaagactcga tgatttggga gcaacaaggt taatcggtgt
      721 tttcagtgta agagtttctc tcttttcagg atgcgttaac actctttttt tcttacactt
      781 ttgctctatt caatctaata taacacagtt tagatatttc aagtaagacg tgcaatgcac
      841 gaagcatcta caaaaaaagc acttgcttac caagcaacac atcggggcat taaagaaacg
      901 gatcttttat taggacgttt cgttgacaat tttttagaaa cgttgagtac acaagaatta
      961 gaaacttttg ctgaattttt aaaaacgccc gatcaggaca tcttggcatg ggtcatggac
     1021 caagaaacac ccccccacgc ttatacatgg atcattaaaa agctgagacc ctgattcatg
     1081 aaaaatgcat actatagtct ttcagaaaac atggaaacgc ttccttttct tgatattttt
     1141 caagatccgc gcgagaaaag ctgtattttt attgtgcatg atgaaaagct tattgatctc
     1201 tatcaaaaac aacttcagcg cattttaccc gaacatttta aaattcttac cctccccgcc
     1261 tttgattgca ttccctatga ccgtgcttcg ccctcccaaa cggtaatgag tgcgcgagtt
     1321 cagacactgc tcgcactgaa aaaagcaaaa gcctttaata agcccgcctg tcttattctc
     1381 tcggtcgagg ggtttttaca aaaagttccc cctcctgaaa cgttttcaga tgatgacctt
     1441 gtgttggggt taggcgatac aatttcaaga gaacgtcttt tgaccttttt ccatgatatg
     1501 ggttttcgcc gtgttgagac cgtttacgaa gtcggtgact atgctgtgcg cggaagtatt
     1561 attgactgct atctccccag tcaaccgtca cctgtgcgcc ttgatttctt aggcgatgat
     1621 attgaatcca ttcgtctgtt tgatgtgttg acgcaaaaat ctcttcaaga gcttgttgag
     1681 gtcaccctaa cgcctgtaag tgagttgttg ttgaatgcta aaaccattga gtgttttcgc
     1741 aagcaattta gaaaacttta tcctatggat gcacctcttt atacggcgat ttctagcggt
     1801 cgcgcttaca gtggtatgga gcacttcctt cctctttttt atgaaaaagt gagttgcatt
     1861 ttagattatg tgcaggatcc tttgctcgtc ctagggttaa atacccccca aaaagcaaca
     1921 gagtgttttg atcgcgctca tctttactat caagaacgtc tagcgccctc tattggggaa
     1981 agaccttatt atcccttaga tccaagtgct ttttatatag agtccgaaac atttgaaaag
     2041 tttgccgcag aagctatcac gtttttcccc catgatgctc tttctcaaaa aagaatggga
     2101 acgattttta cacatctcga tggccttaaa aaagatactc acgcccttgt gaggcatatt
     2161 actgagtgct caaaaacaaa acccttgatt gtaggatgta aaacggaagg tgttctagag
     2221 cgcacacgtg ctttttttca acactattca tcccaggatc ttagcgctga ttttcctcac
     2281 agtgcgccga aacgccttga tcactttgca tcgcttcaaa agcataaaaa tgggcttttt
     2341 ctcatgcttt atccgttgca tgaaggtttt gagacggatg attttatcct tattcccgat
     2401 cgttttttct ttggagaagg gcaaaaagcc aaaaagcgca aagactcact cgatcatttt
     2461 tttaatgagc ttaataccta tagtgtcggt gattttcttg tgcaccgtaa tcatggtgta
     2521 gggaaatatc ttggtcttga aacagtcacg gttgagcgta tcaagcacga ttgccttttg
     2581 ctcgagtata cggatggcaa taaattattt ttgcccgtag agaacatgga tgtcttgact
     2641 tattttgcta aaaatgatgc cgttgtggat cttgatcgcc ttggcacagc ggcttggcaa
     2701 cagaaaaaag ccaaaacaaa agcaaaattg ttagaggttg cagcgtactt gcttgaggtt
     2761 gcggctaagc gtcagttgaa agaaggaatt gttctaggga ctgaatctgt cgactatgat
     2821 gccttttgca aaggttttcc ttatgttgaa acggatgatc aagcccaagc cattgcagac
     2881 gttgaaaatg atttgatctc aggaaaacct atggatcgcc ttgtgtgcgg agatgtaggc
     2941 tttggaaaaa cagagatcgc cttgcgtgca gcctttattg ccgctgcagg cggtcatcaa
     3001 gttgcccttg ttgtccccac cacattgctc gcacggcagc attttgccac ctttcaaaac
     3061 agatttaaac acacaccctt tcgtgtagaa atgctgtgcc gtcttgtaaa acctaaaaaa
     3121 gcacaagaaa tttataatga tgctgtttct ggaaagatta atattctcat ctcgacccat
     3181 gctgcttttt cacataaatt atcttttgct gatttaggtc ttttgattat tgatgaagaa
     3241 catcactttg gcgtgaagca aaaagaaaaa ttaaaaacat tgcgcgcaga tattcatgtt
     3301 cttacactta cagccacacc tattccacgg acgctgcaaa tgtctttgac aggcattcgc
     3361 gagatgagcc ttatcacaac gccgccccta gaccgtctgc ccgtacgcac ctttgttctt
     3421 gaacaagatt ttactatttt gcgtgatgtc atgctccgtg aacatcaacg cggaggacaa
     3481 actttttttg tgagccctcg cacccttgac ctccaagagc tttatcacaa aatcacacaa
     3541 acagcgccag aacttcgtgt ggctatggct catggtcaga tgcctgcagc agaacttgag
     3601 gataaaattc aggattttga tgatcataag tatgatgtgc ttttgtcgac aaatattatt
     3661 gagtcaggta tcgatattcc caatgcaaat acgcttattg tgcacaaagc ccaccttttt
     3721 ggcttagcac agctttatca aattcgcggt cgcattggac gcggaaaaaa gcaaggctat
     3781 gcctatttaa cggtccccta tgatcaactg cttacagaag cggctacaaa acgcctcaaa
     3841 gtccttcaaa gtttagatca tttaggcgct gggtttaatc tcgcgagtca tgatatggaa
     3901 attcgaggct caggcaatct tattggtgaa cagcaatcag gacatatccg cgaggtgggc
     3961 gttgaacttt accagcatat gctgcaagaa gcgattttat cccttcgcac aaaagaattt
     4021 gcagacgagg cgatacgtga cccccttgag gattttacac cgatacttaa tttagggcta
     4081 cccgttctta ttccagaaac gtatattgct gatttatctt tacgcctggg cctttataaa
     4141 cgcttaagca gtttgaaaaa aacagatcac attgaggctt ttgccgctga gctcattgat
     4201 cgttttggtc ctttccccga ggaagtctcc aatctgatta aaattattga tctcaaacaa
     4261 ctttgttatc gcattggcat tgaaaaaatt gatgcaggat ccaaaggtgt tactgttcat
     4321 ttttataaaa ataatttttc aaatcctgag aaattatttc aatatgttca aaaaaatcat
     4381 cctcttctca agattcgtcc tgatcaaaaa cttgttctta cggggaattt agagaatcct
     4441 ttgaggcgtc tcgaactcac aacaaaactc tgtaaggccc ttgaaaagct tgcctcttag
     4501 ttttttaaaa aagcatcgtc tatctaaata atttttacat cataatatta tatttttctt
     4561 attattaatt gatattttac tttaaaatat ttcattagtt agcaataaat taatattttc
     4621 tttctaccat aaaaaagtaa acccattttt ttaacttttt tatggagaac tcaatgacct
     4681 ttatccgtag acttagttta tctctgctca ccgcaggtgc ttttttagca gaagcaaatg
     4741 catcaaacct acaagtcgct caaatcacac caacagaaga gattgatgcc atcacctata
     4801 tctctggtcc ccgtaaacgg ttaacaaccg atcctgttgt cattaaagat gtaaacgcat
     4861 caacatcaac attttatgac aaggcttcta ttggcacagc atcaatgctt accacatcaa
     4921 aaccacaata ttttagagat gacggaaatg gaaacgtctt tgcggcgctt accaatggga
     4981 caaactggga aacctatagc ggcgccattg catcatcata ctcgacatca ggtaccgtta
     5041 acatcacaca aactctttct gatggtgtgt ccgcaacaac aaaaatttta aaaattaacc
     5101 taacaaaggc aacaacaggt accattgcca accttacaca ggccaatctt attgcctatc
     5161 ttgagggatt aacatcagct tcacttttaa ccacaaccgg tggtacgcag ctcacaggct
     5221 ttgtcacacc aacaacgggc tggactatca aacttgccct tgacaatgga tcgggaacac
     5281 tccttgcctc aacacttagc cccgtttcaa gtgctctcag tttaccgatc acaccttacg
     5341 gaagcaatac agcccataac tttaatatta acttgagtct tctcgatact tacaatgtgg
     5401 cgacaaatgc tacaacagga tttgatttaa ccctttttgg aacaacaaat agcgataata
     5461 taacactcac ctgctttcca gcaaaacttg atcgcacaaa agatatccca acatcgatcc
     5521 tgtctatgtt gaataatagc attccaagca agtatttttc ccagactcat tcggagtttt
     5581 atccaagtct ttataatgcg attgttgatc tacgcaactc caaaaaaacc actgttggaa
     5641 acctctcgct gaaggatatg tcaaacctcg atctcattaa tgccagcaac tcaacagcat
     5701 taacatccgc tcggatgaaa acgacctatt catggacatc cgttatcaat gatgactatg
     5761 cctttgtctc aacagcctta agtgatctgt ccagtgtaac atggcgtgta aatggaccac
     5821 aaatcatcgc cactgcaaca gttggaacag acgcccccaa agactttatt tttgacacaa
     5881 gtacgatgag cgcactcgga ttcacaacgg gcaagtggct cccaggacaa gctattctct
     5941 ttaaatctac tgacggggac tctattatcg ccaaaatgtt taacacaagt acaagcaaca
     6001 ccgccggttt agatgccaca tccttcacaa atacaacaga tgaagccatt gacctcgctt
     6061 tactcatgaa agcaaaattt tcaggcgttg taacaaccat taagcatgat tctgaggaaa
     6121 atatccacaa agcaagttac taataaaata agtaggtatt ttaccctaaa agccttcctt
     6181 catttgaggg agggcctttt atatttggaa tgagagagac gaaaaatcgc taaaaccat
//
LOCUS       DAIAHG010000061         6152 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_61_length_6152_cov_3.534156, whole genome shotgun sequence.
ACCESSION   DAIAHG010000061 DAIAHG010000000
VERSION     DAIAHG010000061.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61_length_6152_cov_3.534156"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1830 a         1370 c         1162 g         1790 t
ORIGIN      
        1 aaatacagaa aaatgggcga aaaaagcttc aaaatagtga aattgttgat caaaacaggg
       61 aaattattcc gaaaatcacc aaaattctgc aaaaatgggc gaaaaatcac tttttttcat
      121 gagatccagc gggttttccc aacaatagcg tgtttttcct aacaatagcg tgtttttcca
      181 aaaatcgcag cctcaaaaca gccaaaaatc gcttaaatac gaggcaatta tcctcaaaaa
      241 agctccataa agtgaaaatt tcgggggaaa accctctctt gaaaacagct gtgttaattt
      301 tcacccccta tccttttcag taatttgtcc atcgacaacc tcaagcacgc tatcgacatt
      361 gtgcatagcc gtggaacggt gagaaataaa aataatcgta ggaatgcgcg ccatcacctc
      421 cttaaaaacg tttttctccg ttattttatc catagcattc gtcgtttcat caaaaataag
      481 gatatgcgga ttcaaaagaa tagcccgcgc taaacaaaga cgctgttttt caccgcccga
      541 taaccgtgat cccatttccc ccacaagaga ctgaagcccc aaagggagct tatcaacaca
      601 ctccaaaagt gctgcagcct taagcgcttc gtgaatttta acctcagaaa gtgtcagcgc
      661 atcgacaccg aaaacaagat tatcatagaa cgtgccacta aaaatttgat tttcttgggg
      721 aataaaatga ataactttgc caatttcttc taaggaaagc gtacgaatat cctcccccaa
      781 caaaaaaact ttcccctttt gaggcagcaa aaggcccgtc ataaggccaa ggagtgtgga
      841 ctttccaacc ccacttaccc cataaataag cacacgttga ttattttgaa tcgatagatc
      901 cagatccctc actatttttt tctttttatt gtcataggaa aaagtcacat gctcaaagcg
      961 aatacactca ggagactgca gattaaaatg agctttttga atctcttttt tctccacagg
     1021 cacacttaaa agacctgtta ttttctccat atccacactt gcctcaaaca tttggcgcaa
     1081 atccaaaagc gtttggcgaa tgggaataaa gagtgctgtc atctggccat taaggaggat
     1141 aaaattacca agacttaatg tcccttcatg aactttataa gcccccatgc caatcaatcc
     1201 gcccgtgatc actgccatac aaagccctaa aaacacttgg gcaaaatcca tacgcatcgt
     1261 cacagaaaac cacgcctctg actcttcttg aacaaggttg cgcagacgcc tcatctcaaa
     1321 agatttataa aaacgcacga gttttgtatt caaaatgcta tcgcctaaag ccatggtgac
     1381 tttatctgtg atattccatg cttttgagcg catcacaagg taccaggaca tcgcgaagta
     1441 gaagagacca aaaagcacta aaattccgac cgcaagacca aggcgaaaaa catcaagccc
     1501 ccagataatg ctgaatgcca caataaattt aatgaatgtg ggcagaatcg acacaagaag
     1561 cgcgcgtaaa aaaaggcgtg cactaaatcc aatgcgtttt tgaaaactaa tcacctctgg
     1621 aatcgataat ttctggtaat cactcaaaga gagattgtga acgtgctcgg ttgttttata
     1681 gtgaaattcg cgaatcgtca cattgatgac aggaaagaaa agcaaatcct gcaaaaagga
     1741 agctgtcttt gataaaatgt tcacgcctgc ataaatacca acaagggcta gaagaccatg
     1801 gacagcgcct ttaagctcta gattttcgat gatgttttta aaacaaaatg gcagatacgt
     1861 cacagcaaaa atatcaaccc ccatgacaat aagggcagcc attagaaaaa aacgcgtctc
     1921 cttacaccaa aaatagggca gaaaatagga gaatgttcgt ttgaaagaat gcgtcgagat
     1981 aaaagtaaac ctcttttcga ttgtaacgtg taccgctcga aggatattct agagatctga
     2041 agagggaact tctagaaaaa aataattcta aaaaactttt tcactaaaat tcctcaaaaa
     2101 aaactaaaat taaaaatttt tccattttcc taagaaagta ttaacttttt caacataaaa
     2161 tcagtcgtag aagagggcta aaaagaacct gaaaaggtat tgagagtgca tccattaaag
     2221 ttgtgggttg accccgctct ttcccgttta tgtgagcctt taagtccatt ccttcatcaa
     2281 aaaggatggg cactgcaccc ccatgcccaa acatccgatg cttttctctc gacagaaaaa
     2341 acccttgctc atgttgatat tcttagcctt gttggcgtta ggcccctcat aatttaccaa
     2401 ccacgcttaa cactgcccga attctatctt gaagacctca atgaagtgtc actctctgtg
     2461 acgctccaaa gcattcagcc cctttgcgaa aaactcacta tgcttggcag ccccgcaaaa
     2521 gatgcccatt ttgatatttt ttatgcaggt cttctctttt tatggtcacg cgatagagcc
     2581 attacccctc tcctcgacac aatgcttaaa aagggatttg gttatctcta ttttgatacc
     2641 gttattcata cgctcagacc ggatataagc cttaagggaa ttgatatttt tcgctactta
     2701 gaagaatcct ctcttgtgga aagtgctttt tttgaaacag cccatacctg ccctcgctgt
     2761 gaaagtatca tgctgttgct ccgtgatagt tgccttcaat gtggatcgcc tcatatcgac
     2821 gaagaggtca ttgtccatca cttttcctgt gcttttcaag ccgcagaaag ccaatatctt
     2881 gtcatcaatg aatctctttt gactgaatat gtctgcccca aatgtaaaaa acccctcagg
     2941 cactttggtg tggattatga caagcccggg gttatttatc actgccacca gtgcaatcat
     3001 gataactctg acacagaagt ttacttaaaa tgcatgagct gctcttatac aggcaagggg
     3061 gaggagtcta agaaaataga tattccctcc tttcaaatca cgaataacgg gcggcgtatt
     3121 atcctgggcg gggacagtca cgtctttaat atccgtcgtt ttctaaaaga tcatattcgc
     3181 cttattcctt ttgatacttt tatgttgctt ttacaaaaaa tgagtagcct tcgcgagcat
     3241 aagggaaacg tcattatgac gattactaca ggggggaaaa tcaatgaaaa agcccacccc
     3301 tatgccgcca aattacttat tgaaatgggg aaatccctct cgacttttat ccgcaaaagt
     3361 gactctgtct ccctttatca aagcaatctt tttgtgatgt tcacagatac acatcaagaa
     3421 gcaattcatg acattatgga gattaagaaa aaagagctta aagctatttt cccctccgat
     3481 gtgctgaatt tactgacatt taacatcatg agtggcgcag aatttctcgc acaagtggga
     3541 ggaaatcagt gaaggactat ttagattttg cattgatgct ttattttgga acagaagccc
     3601 atattgtgat ccaaactttt tgggcgattt tcatcgtgac agtgattcgc catcttctga
     3661 gcattatttt cagcagctgg catattcttt tctcaaaaaa tcataatatt cacaacctac
     3721 gccttagggg tgttcaccac gctgcacgcg gtgtcagcat tattcttgcc agtcataata
     3781 cggaccgaga agtcattcaa acggttttat cgctgcgtga gcaaacgatt cctaagatgc
     3841 aaattattct aattaatgat ggttctacgg attttacgga tgcttttgcg cgggatcttg
     3901 tgcgccgaaa gctcgttgat aattacgttg acttgaaggt tcgaggaggt aaagcagccg
     3961 cacttaatgc tggccttgtc ctttcaaagt acgattttac gatgttttgt gatacaggca
     4021 caacttttca cagagacgcg attgaaattg ctttggggta tttcgacgat ccctccgtcg
     4081 gcgctgtctc gtgtggcgta ggtgtgcgca attgtgatga aaacctcctc acttatctac
     4141 aaaaaatgca ttattttatt tcctgtactg tatcacgcaa tgcccttaat tttatgggtc
     4201 ttcagtttgt gatctcaggc gcttttggcc tttttcgcac cgatgtttta agaactattg
     4261 gcagtcattt tcctggcaca ggtgaagacc ttgacctgac acttcgtttg cgtcatttgg
     4321 ggtggcgtgt tgagttttcc tatttctcca tggcccttac ggatgcgcca acaacgattg
     4381 caggcctttt aaagcaaaca ctgaggtggg accgtgatgg cattaaactt gtctatcagc
     4441 gttttgccca ctatgtcatt aacccttttt caagaacctt taatccccat ttggccgttg
     4501 gttttattga taactttatc gtgggagccc ttttaccagc cattatgtac atttacatga
     4561 gttattttac ctttattcac cgggaggggt ttcttttttt tatgttcacg gtctatcttt
     4621 tagaactttt tgttgagctt cttggtgtta ttttttccct catgtttgtt aatcgctttg
     4681 accgcgacta ccgatttctt tactacgtcc cgctttatgt tcttctcaaa caattattct
     4741 atgaacccct ccgttttgca gcgatttggt cagaggttat ttttaaacac tcttataagg
     4801 ataatttctc gccgatgaag gtccaaaaaa tcctcaaaaa agaagaggta cgctaatgag
     4861 tcgttttgcc tcaaaaagaa attgttatgc tgcaccgcat taaaaaacct tcctttatcc
     4921 ataaaatgcc tcaggatatc cgccatcact cctatggaat tagccgtgca gtttaccttg
     4981 gcgttctttt gacgcttgtg ttccttttta ttaattatat gttctatcag tattatattc
     5041 tcgagggtga tgcttttgtt gaatcagaca cgcgtgtgct tgcattcgaa tatgatgcgc
     5101 gcattgagga ggtttttatt gagaacggcg acaaagttgt caaaggtcag gagctcttag
     5161 catttgactc ccttgagttt cgcacacagc ttttaagatc gattactgat ttttcgcaaa
     5221 ttattatgaa tagacaaaat gttgatctta atgtctcaaa actcaatgcc tttattaatg
     5281 cctctgacca ttatttaaat gatgtgcaaa gtgtcaggaa aattcttgaa aatcttaaaa
     5341 aacaagggaa tacaaccctc gatcgcctca ttactgaaga aacaagggaa ttcacagcct
     5401 accgggatta taccggctac aaagcagagc ttgcgtcgaa tttgaaagcg ttgagtgagc
     5461 ttgaggaggt gattagtgtc tctgatacaa cctttgatta ttacatggag tcttactcaa
     5521 aaggcatgaa agttgcgccg attgagggcg tcattggtga tttcaactat gaaccaggcg
     5581 ctgttatacg gaaggcagaa ccgattatga aactctacta tggcagaaga tatttaactg
     5641 ttctttttga agattctctc gttggaaaag aagtcgggga tcccgttatc gtgtctttta
     5701 atgatggcaa tgcttataca attggccgta ttgtggatat tggaaattat acaaaagacc
     5761 tccccgatga aatcaagccc cgctataggc ctcctgggcg cagggccaca gcacgcgttc
     5821 ttgtggacaa acactatctt gacaatcaag atattctcgc aacagcaaag atctacaagc
     5881 cttttttcat ggattttatt tgccgcacag aagactgcaa agcctatctt atcgatgact
     5941 ataaaatcta tctaaaggag aaaaaagacc ttcaaaatca agcacgattt aatcattttc
     6001 aaaggaataa tacacttctt caggttatta cacgacttga taaaaactta caaacacctg
     6061 acccagctcc cagctcccag ctcccagctc ccagctccca gctcccagct cccagctccc
     6121 agctcccagc tcccagctcc cagctcccag ct
//
LOCUS       DAIAHG010000062         5996 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_62_length_5996_cov_4.444839, whole genome shotgun sequence.
ACCESSION   DAIAHG010000062 DAIAHG010000000
VERSION     DAIAHG010000062.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62_length_5996_cov_4.444839"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1684 a         1373 c         1272 g         1667 t
ORIGIN      
        1 aaaaatgttc tgaccaaggc ttttaatgac ctcaggagaa acccctgtgg gcggttgtac
       61 ctccggcgca tgatcatcag aagcgctcga ttcaatcccc tgccacgcgc ctttgagcca
      121 atgggggtga tcaaaacaat cctcacccgc gacagctttt tgataatcgg catcgagacg
      181 ctcttgaaag tctgcagata ggttactcat ctcttcagca gatatcacgc cgtctttttg
      241 aagtttttcc ccataaagag tggctgtgga ggggtgctga tgaatcgctt tatacatcaa
      301 gggttgggta aaactcggct catccgactc attatgccca tagcgcctat agcatatcag
      361 atcaatgacg acatcttgac caaaatgctc tctatagtct acagccgctt tgcacacgcg
      421 aagcacagct tctggatcat cactattcac atgaaaaata ggggcttgaa tggcttttgc
      481 aatatccgaa gagtaaggtg aactgcggga atggggagga cttgttgtaa acccaatctg
      541 attgttaata ataatatgca gtgttccccc cgtgcggtaa ccgggaagat cagagagctc
      601 gagggtttcc gccacaagac cttgccctgc aaaagcggcg tcgccatgca tgagaaggcc
      661 cattactttt tttcgttttg tatcattgaa atgatattgt tcggaccgca ccttgccaag
      721 caccacagga ttgatagcct ctaagtgtga ggggtttgat acaagggaca aatgcagttt
      781 tttgttatca ataacgcggt catttgaata ccccaaatgg tatttgacgt cgcctgatcc
      841 aatggcataa ggattttctt tagctgcgag gaatttagaa aaaagattac gaaagggttt
      901 gccaataata ttggttaaaa tattcaaccg tcctctgtgc gccatgccca taacaagatg
      961 ttccacaccc tgctgagtaa tactatgtaa aatagactcc atcgcaggaa ttagactttc
     1021 gccaccgtca aggccaaagc gcttggctcc ttggaacttt gtgtggagga atttttcaaa
     1081 aagttctgcg cgtatgaggt cattcagaag tgcttttttt tgtggcggtg cgagtgtaaa
     1141 gggaatagat tcaaaaaatt cttggatcca gtttttttta tcgggatctt gaatatgcat
     1201 aaattcaacg ccgattgtgc cgcaatatgt tttatgcaaa cgttctaaaa cttgatgaag
     1261 ggttgctatt tgaaagccaa gaacgccgtc gagataaata agcctgttat aatcagcctc
     1321 ctcgaaaccg taaaatgagg ggctaagctc tgtgtgagga tggcgctttt caattttcaa
     1381 agggtcaaga tccgcatgaa ggtggccgcg cacacggtaa ttgcggatca tcatcaaggc
     1441 acgaatcgaa tcgtgcacag cttgggatgt tgacgtagaa cttatatttt gtgattcaga
     1501 aaaaggtctt gtacttaatt ggtcaaagga ttcccagttg atatcacgac ctacggatac
     1561 ttttgagaaa agatcacccc attcagaggg aatgctctga ggatcacgct gataggcctc
     1621 gaataaactt tcgatataag gagcattatg aatataaggc gatgaaatca tttaaaaacc
     1681 tctttggtta tccttaaaag aaactaatgc gacaaaccct ttttattttt atgatgttct
     1741 tgatgtgcgg cattatggat cagatcggcc atcatttttc ccatacgtgc agggctttcg
     1801 cacactgtta cgccacattt ttcaaggtaa tccatttttt ccaaagcaga accaccggca
     1861 ccggcaataa tggcgcctgc atgccccata cgacgccctt taggcgctga ccgtcctgca
     1921 ataaacgcaa ccacaggttt ataatttccc gcgtcatatt gggctttgat aaagtcacct
     1981 gcgcgttgct cttcgtcgcc gccaatttca ccaatcatca agacagcctc tgtggcagga
     2041 tccttccaaa agagctctaa aacatccaca aaacccatgc ccttgaaagg atcaccacca
     2101 atgccgatac aggtggattg gccaagccct tccttggttg tttggtcaac ggcttcatag
     2161 gttaaagttc ccgagcgcga gacaacccca acctttccag gggtgtgaat aaaaccaggc
     2221 ataataccga ttttgcattg ccctggcgta ataattccag gacaattggg cccgataagc
     2281 cgtgacttag aatttttgag tgcccagtgc actttcatca tatcttgaac aggaatacct
     2341 tctgtgatgc agataatcag cgggatttcc gcatccatcg cttcaaaaat agcatccgcc
     2401 gcatagagag gagggacata aatcatcgtt gcatctgcat taacagcatc gacggcttgt
     2461 ttgactgtat caaaaacggg gaggcctaaa tgcgttgatc cgccctttcc aggtgtgaca
     2521 ccgccaagga gttttgtgcc ataagcaaga gcaccctctg agtggaaggt tccatgtttg
     2581 cccgtaaagc cctgacagat aaggcgtgta tttttatcga caagaataga cattttttag
     2641 tttcctttcg acacatgaac aacggttgca gccgcgtttt caaaatcatc ttcagcaatg
     2701 attttaagac cactttcatt taaaagggcg cgtccttttt cgacattcgt tccttgaagg
     2761 cgtaccacaa ggggaacctt taaattaatt tccttggccg cagcaataat gccttctgca
     2821 ataatgtcac agcgcataat gccgccaaaa atattgacaa aaatagcacg tacatcttga
     2881 tcatccgtga tgattttaaa agcttctgtg actttttcct tagttgcgcc accgcctaca
     2941 tcaagaaagt tagcggggct cgcgccgtga cgggtgataa tatccatcgt tgccatggca
     3001 aggcctgcgc cattaaccat gcagccaatc gttccgtcca gttttatata attgagatca
     3061 aattccttcg cacgaagctc tgcggggtca acctcattct catcaaaaag aaagctcacc
     3121 tttttttgtc gcgcaagacc attatcatca aaagtcatct ttgcatccaa cgcgagccat
     3181 gtgtgatctg ttgtgaggac aagtggatta atctcaatta gctccgcatc aagagttgta
     3241 aacgcctgat acattgattt gaggaggttc atcaactctt tttgcgattc tttggggaca
     3301 cctaaaaatg tcacaagacg acgtccatga aaaggctgat accctacgac aggatgaatg
     3361 cccatataga gaactttttc aggcgatttc tcggcaacct cctcaatatt gacgccacct
     3421 tctgcggatg caatgaccat aaatcccttg tttttacggt cgagcacaag gcttaaataa
     3481 tattcgtgtt ttatcgagca tgtatcttct atataaaggg ctctgacaca ctggccctct
     3541 ttttttgtct gtgctgtcac aagcgtacgg cctagaatgc tctccacagc gctgtcaaag
     3601 ctggctttgt ctttggcaat tttaacgccg ccagccttgc cacggccgcc tgcgtgaatc
     3661 tgagccttga caaccacacc tttagcgagg tcaaagtgag aaagggcgct tgttgcctct
     3721 tcttttgaaa aagcgacgcg gccctcaagg gtagggacac caaactccct taaaagacct
     3781 ttgccctggt attcatggat gttcatggtg acgctccttt gttttttgac agattaaaat
     3841 ttcattaaat attaaggcgc tcgaggtcat gaataaggcc ctctacagct tttgcagagc
     3901 ggtgaaacgc tgcttgctca gcctctgtca gatcaagctc aataatatct tccacacctt
     3961 ttgcaccgat tacacaagga acgcctacat aaatatcctt gacaccatag gcatttttca
     4021 catgcgccgc acaaggaaga gctctttttt gatcataaaa atacgcctca agcatttgaa
     4081 cagcagaatt cgccggtgca taaaaagcgg agcctgtttg gagaagattc acaatctcag
     4141 cgcccccatt gcgtgtgcgc tcaataatgt catcgacgca tttttgagtg atttttcctt
     4201 tgtcaataaa atgattcaaa ggaatgccca gaaccgttgt aaatccaggc ataggaacca
     4261 tcgtatcacc gtgtcctccc agcacaaacg catcaatcga ttgcacagat acattaagtt
     4321 tttgcgctaa aaatgtttta tagcgtcctg tatctaaaac gcccgccatg ccaataacat
     4381 gggttgtggg caagccactt gcttgctgca tcagccagac cataatgtcc agggggtttg
     4441 taatcacaat gacaaaggca ttggggcagt gtttttttat ttcgagagcc gttgtcttga
     4501 taacacctgc gttaattttc aaaagatcat cgcggctcat acctggctta cgggggatgc
     4561 cggctgtgac aatgactgcg tcgcatcctt tgagaagccc ataatcactc cctccctcaa
     4621 tcacacaatc atggccagag accgcaaaac tttgctgaag atcaagggcc tttcccttgg
     4681 ccataccttc tgcaatatca ataagggcaa catccccaag gtcttttaga ccaatgagtt
     4741 gagcaagtgt tccgccaatc tgaccggcac ctacaagccc tatttttcga cgcttcatca
     4801 tcattatttg actcctttaa ctttgtacta ttttttaaat ttcggcactg ttattattca
     4861 tagcaagaat tggtgaattt ttagtaaaga ttttctggat aaattctaag ccgcggtgca
     4921 agccattcat atcaatgcta tgcccaagtg caggacctgt ttctgtttca atctcacacc
     4981 caagatcttc aagatctttt ttggccttcg ccatcagcgt ataggggaca cttggatcca
     5041 aagccccatg aacaagaaga acagggggat agggaccctt gaattttatt ttagagggcg
     5101 gataaaaagc gcccgaataa cttataatgc ctgcaagatc tggaatatga aacatcattt
     5161 caagggctaa catcgctcct tgggaaaatc ccacaagggc gacatcgcgc ggcgttaaat
     5221 catagtgatg catttgtgaa tgaataaaat acgataactt ttccgaggca tagtctaaac
     5281 ctttgcgcat gtctttggga tccagatcat gatttggaat gctaaaccat tgatagccaa
     5341 aggggcccgc ctcccaagga tcgtgcgcat tgggtgaaat aaatgtggta agacgcaaag
     5401 agggtgccca aaaatggcct atctccaaaa gattagcgcc attggatccg tatccgtgaa
     5461 gaagaaaaac aaggcgcttc gtttctttat cactagagaa aacataatca ggagtggtca
     5521 taaaattaaa aaaaacggca tatcctattt ttatggtact gatcttgctc tgaggacaca
     5581 agggggaaag attttttctt ttggtcgctc ttgaaaaaaa cgaagtgagc agctcgagtg
     5641 ttaacatcgc tccttgggaa aatcccacaa gagcgacatc gcgcggcgtt aaatcatagt
     5701 gatgcatttg tgaatgaata aaatacgata acttttccga ggcagctttt tctcaaatta
     5761 tgagcctaaa acattaacct tttattcaca caccttaaca aaaagttaat tttcgcaccc
     5821 ttctttatga tggtattttt cgttatatag taataaaatt ttatgaaatt gtgaaaaaac
     5881 aggtgaaaaa gggtcacttt ttccgatgaa acacaccgct tttaagggat ttttggctgt
     5941 tttaagtctt tgattgtcga aaaaaatcat ctatccacca aatacagtga tttttt
//
LOCUS       DAIAHG010000063         5995 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_63_length_5995_cov_3.189929, whole genome shotgun sequence.
ACCESSION   DAIAHG010000063 DAIAHG010000000
VERSION     DAIAHG010000063.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63_length_5995_cov_3.189929"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1651 a         1246 c         1474 g         1624 t
ORIGIN      
        1 agaaccttct ttggaggaaa aaaggtccta ttctcatgcg gcgcctacac agacaagtag
       61 ctcagctcct gcatcgttgg caaagaagca agagtcaaag gcgcgtgtgg aaagtcagcc
      121 ttcaaaaaat acctcgtctg agccgcgtat attcatggcc catgatttta agccctcagc
      181 acggccggcg gctccttcaa agactgaaac aagaacatcg cctaaagctc ttttggatga
      241 tgattttgat gtaacaggtg aggggagtaa aaaaagaaca cagcgtgtgg atacgaaaaa
      301 agctgttgcg gctccgacaa cggctgtgaa aaaagatagt gctcttccca aaaaactaac
      361 gggttcgtct ttgacacgtg cactcaatga agagtccgat cgtatgcgtt ccatggcgtc
      421 ctacaggcgc gcgaagcaaa agcaaaagca atcaggggtt cagaccgagg ccattaaaat
      481 tacgcgcgaa gtgattatcc ctgagacaat gagtgttggt gagcttgcaa atcgtatggc
      541 tgtgcgtggg gctgacgttg taaaaacact gatgaaactt ggtatgatgg tgacgattaa
      601 ccagattatt gatgcggata cagcagaact tgtgtgttct gagtttgggc acacacctaa
      661 acgtgttgcg gattctgata ttgaaattgg tttgcgtggc gcagaagatg ctgttgatct
      721 tcttgtctcc cgtgcgcctg ttgtaacggt gatgggccac gtggatcatg gtaaaacgtc
      781 attgctagat gcgttacgct caaccgatgt tgtcagtggt gaggccggtg gcattacaca
      841 gcacatcggt gcttatcagg tgacgattca atcgggtcag aaaattactt ttatcgatac
      901 acctggccac gcagctttta cagaaatgcg tgcccgtggt gcagacatta ccgacgttgt
      961 tgttcttgtt gtggcggcgg atgatggtat tatgccgcaa acgatcgagg cgattaatca
     1021 cgcaaaggct gcgaatgtgc cgcttgttgt ggcgattaat aaaattgata agcccgaggc
     1081 tgatcccacg cgtgtgcgct ctgaattgct gcaacatggt gttgtccttg aggattttgg
     1141 gggcgatgtt atggctgtcg aagtttctgc gaagaagcgt atgaaccttg ataaactcga
     1201 ggaagctgtt cttcttcagg cggagattct tgatcttaag gcgaatccta accgtgatgc
     1261 agaaggtgtt gttgtggaaa gcaaactgga gcgtggacgg ggtaccgttg ccacaattct
     1321 ggtgcagcgt ggtacgctga aaataggtga tatttttgtg gcgggtaaag aatgggggcg
     1381 tgtgcgggcg cttgttgatg atcacggcca gaaagttaca aacgctttgc catcgatgcc
     1441 tgtagaggtg attggcttta acggtcttcc tgcggctggt gatgagttct ttgttgtcga
     1501 atctgaaagc cgtgcacgtg aagttgctga gtaccgtgag cacaaagaaa aagaagccaa
     1561 ggcggcagct gtttctaagt ccaatatgga taagatgatg aaccgtattg cggcaagcaa
     1621 tatcaaagaa cttgctgttg tgattaaaac agacgttcaa ggctcccttg aagcaattac
     1681 aagtagcctt ttgaaaatgg cgaatgatga ggtttctgtg cgtattcttc acggtgccgt
     1741 aggcggtatt aacgaaagtg acgtgacgtt ggcacgggca tcgaatggtt ttatcgttgg
     1801 gtttaacgtg cgtgcaaatc ctcaagcacg tgatcttgcg cggcgcgatg ctgtggatat
     1861 tcgctattat tcaattattt atgatgctct tgatgatatt aaagcagcac ttggtggact
     1921 tttgagcccg acacttaagg aaaaattcct tggctatgcc gaaattcgcg aggtctttaa
     1981 tattacgaaa atcggtaaag ttgcgggttg ttatgtgact gaaggactcg tcaagcgcgg
     2041 tgcaaaagtc cgattgctgc gtgatgatgt tgtgattcac gaaggtgccc ttaaaacact
     2101 tcgtcgcttt aaagacgaag tcaaagaagt caaagatagt tttgagtgcg gtatggcctt
     2161 tgagcattat gatgatatta agcaaggcga tgtgatcgaa tgctttgaga ttgaagaaat
     2221 tgcgcgcgct ctttagtaac gctcgtgtat ctcttctgta aaaagaagca taaaggagat
     2281 tctttgtgga atggataacg tctcctgcat acgttgatta tgatgctgcg gaggctttta
     2341 tggatgcgcg cattatgggc atccaacaac aaaaactctc tgagtgtgtg tggcttttag
     2401 agcattcacc gctctacaca gcagggacga gtgctcaagc tagcgattac ctcaaaacgc
     2461 atgatcttcc cgtgtattcc atagggcgag ggggaaaata tacgtatcat ggtccagggc
     2521 agcgtgtctg ctacgttatg ctggatcttt caaagcgaac acgtgatgtt aaaaaacatg
     2581 tgtggagcct ggaacaatgg ggcattgagg ccttacatct ttgtggtgtt acggcatgcc
     2641 gggacgaggc aggcgtcggg ctttgggtat cgacagaaaa aggatcttct aaaatagcgg
     2701 cgattggcgt gcgcgtgagt aaatgggtta caaaatacgg tattgctttt aatctcgctc
     2761 cgaatcttga gtattacaat ggaattatcc cctgcggtat tgagggcaaa ggtgtgacaa
     2821 gccttaaagc gctggggatt gatgtgacaa tggatacttt tgatgcgtat ttaaaacaaa
     2881 cctttgcgcg ttcttttgca tagaattttt tatcactctc actaacactc aaagcctgta
     2941 tcgttttcct caaggatcat gcgatcatac gccggagtga cgttttcagg gcaccgtgaa
     3001 aaaatatcat catagtcgat acgttcacac atgtgcgtta aaattgctcg cttgggttga
     3061 aactgtttga tccaatccat agtgagctca aaatgcgcat gggtatgatg ctcttcatag
     3121 cgcaagcaat ccacaatcca aagactcaga tttttcagat aaatctccga ctcctttgga
     3181 aaacttttta catcggtaga gtaggccatt gatccaattt taaagcccga ggtttgttga
     3241 tagccgtggg attgttcaaa cattgtaatg ggaagatcat caaccataaa tgttccaggt
     3301 ctgatcgcat ggcctgttaa atattctgga taaatggggt gagtcgatat gaacatatag
     3361 gaaaacatac tttgcacttt ttccagcgtc acagcctcag cgtaaacagg aatttttttt
     3421 tgctgatgaa agaaaatttg gcgaagctca tccaagccat ggcagtgatc cgcgtgagca
     3481 tgcgtgataa gcacagcgtc aatcgtattc accccaatac gtaaaagttg ctcgcgcaaa
     3541 tccggcgagg catcaatcaa aatatttttt tctttgacct ggacaaggat agatgtgcgt
     3601 gtgcgacgat ttttaggatt atcgggattg caacgtcccc agttccccga aataagcggc
     3661 accccaccag aggaaccaca tcctaaaaca tgaattttca taagattacg atcatccttt
     3721 ttttttgaat tttttattat catgattcat ttttaactaa gcaacctgat cctcttgatg
     3781 cgtgtctgga taaaggccat agcccacatg acgcaccgtc tgaagatagc gtggctgacg
     3841 gggatcatcc cctattttcc ggcgcaggcg tacaatttgg acatccacac tgcgttcgct
     3901 gactctgtgg ccaatacgct gtgcaagttc ttcccgtgta aacggttttc ccggtaattg
     3961 tgcaaaaatg cgcagcaacg tgagctcact ggacgtgaga aaaacagctt ctgtgccttt
     4021 gagaagagta ttacttttcg tatcaaacgt gtagtcaccg agagagagac gggaaatttc
     4081 ttttgtagtc tccggttgtt ttcggcgaag aagagagcga atgcgcgcaa ggagctcacc
     4141 gggatcaaaa ggtttggaaa gataatcatc cgcaccagcc tcgagacctt taatacaatc
     4201 actcacagta tctttggctg taaggagtaa aatgcccaca tctttatagc catttgatct
     4261 taggcttttt gtgagattca tgccatcctc gccctccatc atcacatcca ataccatcac
     4321 atcgatgggg tgtgtggcaa gcattgagcg cgcctctgca gcactcgctg cttcgaaagt
     4381 atcaaagcct gagtctttta aaaaacttga tagaagctgc cgaagccgtc tgtcatcatc
     4441 tacaacaaga agctgagtca tatcttttaa aaacatctct acatagttac cttgataatt
     4501 taagccattt taaggacaag aggcaacagg atcattcaat gtcatgagcc ttatgcgtgt
     4561 attttaatgg gtaaccatga ctcctcagtg ttaatttttt caaaattatg aaaaaaatga
     4621 agcagtttgc ctgtgttccc tgtttgaagc cattagagtc taagccgctt tttggagttt
     4681 cagttccttc catagaaagc ttagggcttg gataaggcaa tccatatgct cctttgtgtg
     4741 ttggggcgtg acggtgatgc gcaagcgttc ttcgccaaga gggaccgttg gaaaattaat
     4801 gggctgaatg taaagaccgt aatcggtgag gaggcggtcc gagagttcct tgcatttaag
     4861 ggcatccccc acacggatgg ggataatgtg gctatcattt tgaacaatgc ccacgcctga
     4921 tttctcaaaa tgctctttaa gatagaaaac atttgcgaat aactgccgac ggtaatcatc
     4981 gcaatattgt tgaacctcaa cggatttaag gcaggcggcc gccacagcag gagggatgga
     5041 ggttgtaaaa ataaaacctg gagcgaaaga gcgcacaaaa tccacaagat tgctttgtga
     5101 ggcgatatac cctcccacaa cgccatagcc cttggcaaag ttggcctgaa taatatcaat
     5161 atcgcccgtc aatccttcag cagcagctaa accagcaccc caagggccat agatacccac
     5221 agcatgcact tcatccaaaa aagtcaacgc attgtatttt tttgccagat aaataatatt
     5281 ttgaatgggt gcaaaatcac catccatgga atacacggac acaaacgcaa tgatttttgg
     5341 gcgctcaagg ggataggatt ggagcatttg ctcaaggtga tgcatatcat tgtgcttaaa
     5401 cacgcgcttt tctgtgcggc tatggcgaat gccgtgaatc atcgacgcgt gatttttttc
     5461 atcactaaaa atcacacact caggaatttg ggatcctaaa atatgcaaag aagctttgtt
     5521 ggcggtatag ccagaggaaa aaagaaggcc tgcttccttt ccatgaagct cggcaacttt
     5581 gcgctctaaa tccacatggg cctttgatgt gccagaaata ttacgcgtgc cgcccgatcc
     5641 cacaccataa gtgtccgctg cgttgtgaaa cgcttcaaga acatctgggt gttggctcat
     5701 gccaagatag tcattactgc aaaacacaat gaccctttcg gttgaaccat cttccttgtg
     5761 ccacaaggca tagggggctt gtccgcaaag ccgctcaagc gatacgaacg tgcgatagcg
     5821 accatcttct ttgatcgtgt taagggattg tgtaaaaaat gtattatagt ccatagagtc
     5881 tttaaagagc acctcgtgat ggggatatta taatcacctt tttattaatg tcaccttaag
     5941 attttaaaaa aattatcaat aactataaag aaactacctg tgcataaaaa acaaa
//
LOCUS       DAIAHG010000064         5966 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_64_length_5966_cov_3.056037, whole genome shotgun sequence.
ACCESSION   DAIAHG010000064 DAIAHG010000000
VERSION     DAIAHG010000064.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64_length_5966_cov_3.056037"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1668 a         1231 c         1130 g         1937 t
ORIGIN      
        1 agcagctcaa gtttttgcat gaccacgcga aaaaagatct cgatgctcat attcaagatg
       61 tgattgaaga taaaataaaa tcagaggttg acgcgctcac aaatgctaaa gagcctgaaa
      121 cggtaaaacc tgcagaaaaa cttgatacga aaaaagcaac cgagaatctt gcaaaacttc
      181 aggttcaaac acctacaaag caaaaagatg ggtatgataa agatcaacaa ggcgacctca
      241 atcgccttat tgaaacggtg cgataaaatt acctaaaagg ttttgccttt attcataaat
      301 tctcttagca aaatttccac ggtcaggatt attaatgcct gtgtctgttg tgatatttat
      361 tgtcgctttt gaggagttct cgccaaaggc tttgttaaag atttttttcc aggtattgcc
      421 aataactttg ctatttttcg ctaaaaagag attaatattt tgcatttcgc ctaaatcttt
      481 tatggaactg aggagctctt tgaccgtctt ctcatagtct aaataactaa atttatagtt
      541 accttcatca tcaatgcctg caccatcaat atagagcgtt aatttcttta atccatcaat
      601 tttttctgga ataacatgtg taaaacgcac ttcctttgaa tcatttggat aatacatatt
      661 aaaaaagata gataatttct tatctttaag tggcaattga gtgatgaaat ccttcaaagg
      721 atatttatta ttttcctttg ttgtctcaaa agttttttgc gatgcatttt ctaaatcaag
      781 agaaacatta ctaattgcaa tcttttttaa aaattcactc gatggaaaat tactcagatc
      841 tgtgttgggt tttacaacaa tattaagcgg tgatttattt tcctcatcat tgaaatagcc
      901 attaacataa tcttcattgc gagtactaat caaaacttta tgtttattgg gattaaaagt
      961 ctgttgtagc atgccctgct caccaatttt tagattacca actctactag ggttaagagt
     1021 atttttttca taggtaattt ttttcaaaac ggactcaact tcttgctcgg agtatccgta
     1081 ttctactttt cctagaggtg tctctgattc cttcatcgcg aaagtttgtg gtgctgtaga
     1141 acagagaaga aggccaagca gaagaccttt aatatactat tttttcatcc tatttccttg
     1201 atttttgatg ttattgaaac aaataagata taagttataa atttattttg atcaatattt
     1261 ttataagtcg tgtctttaca ctgacacatt tcgttacgtt ataaacattg cacaaagagg
     1321 caaggttttt ttataactaa tatagaatcc actatcatta atatttttat gcattttatt
     1381 gatcttcaag cacagcaaaa acatattcgt cgcaacatcg acgcgcgtat tcaaaaggtt
     1441 cttgaccatg gggcgtatat tatgggcccg gaagtgtttg agctggaggc taaactttca
     1501 gaattttgcg gcgcaaaaca tgtgctgagt tgtgcaaacg gtacggatgc cctcggcctt
     1561 gttcttatgg caaagggtgt ttcaaagggc gatgctgtgt tcgttccaac gtttactttt
     1621 gctgccacag cagaagttgt tgcatgggtg ggagccacag ctgtttttat tgattcagat
     1681 cctgcgacat ttaatatgtg ccccaaaagc cttcagaatg gtattgaaaa agcaaaaaca
     1741 cttggtctta agccaacagc cattattcct gttgatcttt tcggccttcc tgcagatttt
     1801 gatgcgcttg aagcgattgc acagaaaaat aatctttgga ttctcgatga tgctgcacag
     1861 ggattcgggg gcatctacaa aggacgtcat attggtactt ttggccttgc tacaaccaca
     1921 agcttttttc ccgcaaaacc ccttggatgt tacggcgatg gaggagcgat ttttacggac
     1981 aatgatgatc ttgcaactac tcttaaatca cttcgtgttc acgggcaagg caccgataaa
     2041 tatgataata ttcgcattgg catgaacggt cgccttgata caattcaatg tgctattttg
     2101 cttgaaaagc ttgctatttt tagagatgaa attaaagcgc gcaacgagat cgcaaagcgt
     2161 tataacgaag gactaaagga tcacgtccat gtgccagaaa tttttaacga tatgacctcc
     2221 acctgggcac aatacacaat tgttcttgat aaaaatatag atcgtcaaaa gctgatggat
     2281 gccctcaaag caaaaggtat tccaacggcc gtttattacg gtcgtccgct gcaccagcaa
     2341 acggcgtata aacattatcc aactgccacg gaaaaccttg ctgtgacaga ggatctttgt
     2401 tcccgtgttt taagtctgcc catgtatccc tatttagaaa aagatgcaca agattttatt
     2461 attgagacta ttcaaaaatc ccccacactc gtctaagagc atgaggggag tgggatacta
     2521 tctctcttca gagagtttat tatagataat ttttacgagt gagtctgaca ctgccttgat
     2581 acttttgttc gcaggtaatc cagcgagaag tgttctcttg ccaaaaataa gttttctcaa
     2641 taattttttg gttgtttgaa gttttttatt ttctgaataa aaaattgagt tcatagcatc
     2701 acttgccatg acggacacat tcgcaatgta tttatattct tccccatctt tttcaacctc
     2761 attattttcg atcaggtagt tgtgatcagc gagcacgcaa acacgggggg tataaatatt
     2821 ttcatttttt gagatattct ccactcctat gaaaacgggc gctgttaact ctttagaagg
     2881 acgaaattct tttatagact tttctttatt ttcttttaaa tcatcacttg cacgcgtagc
     2941 attagcagtg aagcagcaag ggttgtgtaa atatagcttt tctttatggt tttttatcct
     3001 taatcagttt taaccatcct caacatagag aattaatcgt ttactaagaa taaaaaaatt
     3061 attgtttgat gagagtagga tgatgaccaa aaatgtttaa actcgtcagc gccatctctg
     3121 ccgcatcttt attggcataa ggaccacacg tcacagtaaa aaacttttgc ttgcctggca
     3181 gaatgatttt ttcttcaacc tttgcttgag caagaccttg aatttcgccc acaagttttt
     3241 cagcattgat gcgattgaca aaggacgcaa gttgaatttt ataggaggca tcaaatgaat
     3301 ttttctgtgt ttttttcaaa tttttagcaa cttgcggcag ggcgcttttt gcatgttttt
     3361 caataaggtc atcaagactt gcacttggaa catgtatagg caccacatcc tcggaaggtt
     3421 tatgcccatg tttataccca gggacagggt gatgggaccc agaccccgct tcatacactt
     3481 catcagaatg gttgggcgca atatcttctc gataaacatc ctcccaaatc cgccccggta
     3541 cagcgcccca tttgttccca atttttgcaa ggtgcatagc aagcgcatgg ctttgtgcgg
     3601 gcaccgattg cacacgaacc tttgcggttc ctttagcata cacaccaatg gcctttgctg
     3661 cccccatgga taaatcaata atacgcccct cataggtata aggacctctg tcatcaacca
     3721 caaggtaaat catttttcca ttctcaaggt ttgtgacttt tacaaccgtt ggcagaggca
     3781 atgttttatg ggcagcgctc atccctgttt gatcaaaaat ctcaccattg gctttttgtt
     3841 ttccatgaaa gcgtgggccg taccaggatg caatggctgt ctcgtcataa tcataaaaaa
     3901 gctgtggcgt ataccacgct cctctgacca tatggggttt gcacgaacga catacaccct
     3961 tgacagggta ctttcccgtc ataatatgac caccaccaca tgctgtaaga agcaagagtc
     4021 ctagaagaga gacaaaacgc attttcagag ttagaaaggc tcaaggaagt ttatgggaag
     4081 actataccag gtagattttt tcttagggaa tttaatttca aaaatctttg aggggatatt
     4141 aacttttctg caagatattt aggcgcacct gtatctaaat ctcctcaaag gatgtcttgt
     4201 aaaatttttt ttatttctca ggatataatc ggttcttaag atcaaaggat ggcttgcaaa
     4261 tttgtttcaa ccttgggaac agtactcaga aacacaccca atgcccccac caatcaggcc
     4321 accagcgact ccaccaccta tgcccatggg aactccagca gcaactccaa gaatacagcc
     4381 tccaacggcc cccgttactg tatacgcact gagagagata ggatcagaag gataatattt
     4441 caagaataat aaatatgctg gtagcccaaa gaaaccagaa actgtaccta gaatacctgt
     4501 gcccataaaa ccagctgctc ctccaaaggt tgcgccatta gcagcgtagg aggaaatttt
     4561 attatctttt tgctccacaa caattttttg tgtaaatttt ttattttctt cacttatgtc
     4621 gtcgcactca gatgctgata ctgacgatac caagataaaa agcgtggcga cgacagaaaa
     4681 aatcatttta aggttcataa attccccgcg actaagaacg cttgctctaa ataactttac
     4741 taaaaccaag ataaatttaa aatactttta gtacatatct tttggattga aacgcagggt
     4801 cagcctcttc cacttttcat atttctcagg agggagaggg agtggattac acgccggatc
     4861 caaaacggcg cgctgggcac tttcggcagc cgcttgaaag gcaggatcac cgctgcgact
     4921 attcacaacc tccgcctttg tcacattacc gtcttgagca atatccatgc gcacatccac
     4981 cacaagatct ttggcgcctt ggacaccgat agggataatc caacatttat aaattttttg
     5041 acggatagca tcaatatctg ttgctgtaat ttccatgccc acagccgacg caggcgcacc
     5101 tttttctttg ccctcctcct ccaacgtatc gtcgataaga ctgttgacaa tgtcatccag
     5161 ggattttttc ttctcagaag aaaggggctt aggttttgtt tttttaggat cgagattaac
     5221 ttctgccttt gtgggcttcg tttctttctt tttatccttt ggcgcatctt tcttaggttt
     5281 tgattctttt ttttcttctt taggaggatc ctttttgggc ttttcttcct ttttcttatc
     5341 cagcacagga tctgtttctt tttccttcat ttcctctttt ggtgcagact ttttttcctc
     5401 aatgacttct tcttttttct cttcgatttt ttcttcaggt ttctcctctt ttttctcctc
     5461 ctgaggttct atttttttct caactttttt aattgttttt ttctcttctt ttgacgcatc
     5521 gggttgaagt ttaggtgctg cacttttgtc tgcaatcttt tcaaactcaa gaataatggg
     5581 ctgttcattc accggaacag tacgttgggg cagcggaatc cccacaagaa gaatcactaa
     5641 aaaaaggata tggaaaaggg cagagaaaaa agccgacttt ttcatgctaa aggtctacct
     5701 cactgccttt ttcttcaacg gctgctgctg ttttttagaa ggagatgatg ttttttctgg
     5761 cgtattgttt gatgtatttg gggtgtgcgc cgggagctct gccagtagcg atacctttgt
     5821 aaaaccggct gttgcaatgg atcccatggt ttccataatc tgtccatagg taagattttt
     5881 atcgccacgc acgtaaattt tagcctcggg attattattt gtaatcgcca tcagtcttgg
     5941 aataagttct ttcatcggca cttctg
//
LOCUS       DAIAHG010000065         5964 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_65_length_5964_cov_2.671819, whole genome shotgun sequence.
ACCESSION   DAIAHG010000065 DAIAHG010000000
VERSION     DAIAHG010000065.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65_length_5964_cov_2.671819"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1590 a         1090 c         1284 g         2000 t
ORIGIN      
        1 actgatgcca tgatcatgat tttgttttaa tttgacgaaa taaaaagatc catcgtgtgc
       61 gtgagcttta taggtgagtg cgtcgatatc agcgccgagg gaaagccgtg taagcgttgt
      121 gacatcaata ccgtacgcat ttttgaggcc atcgataacg tgtgtgtctg agggagggag
      181 tgtgcgcttc ataaatggat tataggcaga gagtgtgcta taagaaacgg tttttttcgt
      241 agggtgtgag aggctaatgc tctattagaa aagtttttct ttgaattcta gggaaaaata
      301 aatataattt tagataagtt atgtttttgc ctgcctgtcc atgaacagtt ttgagtttaa
      361 taaaattttt gcggcgctcc ttgtagctct tcttgttgcg atgattgcaa accttgtggg
      421 agaggaagcg gttcatcctg caaagatcgc taaaaatgtt tttattattg atgtggcaga
      481 ggcgccggtg gaaagtgggg gcgaggtgaa agaagaaaaa gttccgccca tcggtccttt
      541 ccttgcaaag gcaagtgttg caaatggtga gaaacttgtg aataaattgt gcggccagtg
      601 ccatactttt aaagcgggtg agcctgcaaa agtagggccc aacctttttg ggattgtggg
      661 gaataccttt gcgcacatgg caagttttgc ctattcgcgc gtgttccaag agaagcatgg
      721 caaagacacg tgggatgagg aaaaactaaa tatttatctc cacaaaccgc gcgcgcttat
      781 gccggggaca aagatggcct tcgcagggat taaaaaaatc caagaccgcg ccgatgtaat
      841 cgcctacctt aagtcactta agtaaggtac gatgattaat tctcattctt atttttatta
      901 ttgacttcct ccactataat atctctcaga acggattcca tataaaggta cattttaaat
      961 tttttccata actcctccag ctcttctatg agagactcct ttgtactacc agataaattt
     1021 tttgcatttt tacgaaagta tttttctttt tctaatgcct ctttagatac tttatccgct
     1081 tttttactga gggcttcaag atcgagaagg tcattttttt ccaagaattt ttttgtgtct
     1141 tttttaaaat gttcctcaag gccttttatg ctcaaaatag agaaaagcgt aagctgaata
     1201 gaggcatcat cggatagatt atagtggttt ataagattca aagctttgta gcatgcgtta
     1261 gaaaaaaaat aaccctttgt ggatgcttta gctgcagcaa ggagtatttc gctgactaaa
     1321 atgtcgattg gatattcttt agaattcgtt ttatattttt ttgcagtttt catccaatgc
     1381 tttagatcac caggttttcc attcgtgttt tttaaaagcg catagagtaa ctggatagtg
     1441 tagtcattat attgcttatt cccgatattg ttcaaacatt gttctatctc agagaagtcg
     1501 tttgtttgtc tcgtctgaaa ctcaccattc ttatcatcta tttcaatgta ttctgtggtg
     1561 tcttgagaaa ttgtgaaaaa atcttcgccg agttcttcgt taagttcctt aatagtctct
     1621 tcatcgagtt ctgccttgtt ttttatttta aatttaatat cctgatcttc tatatcttca
     1681 cctgttttgt gttttttagg gttaaaattt ttagaaaaac tgccattctt gagctcagtt
     1741 ttgatttttt tagactcgat tttttttagg ataagaattt ttgggttctc ttccttccat
     1801 gcaaaaactc tgtatccctc atcctcagca tcctcaagcg tgagatcttt ctttttttca
     1861 atttttatat catcaaagaa atcaatataa tgttcacccc cagcgaaaat atatttttct
     1921 tttttctcct ctttttttat cttgttagac agattagtcg tgagtttttt ctccgaaaaa
     1981 atatttgatt tttcctcgga cttatccgtt acgatgccta ttttttcgac gattttattg
     2041 ttttcttctt tctttttcat cacaaggaat tgagaaggat tttctttatc ttgggcaata
     2101 attttatact cattctcaag aatatcgggg atagttgttt cactattgaa tttaaggaaa
     2161 ttcttatttt gataggattc attaaaataa tcttgaatat taaagaatgt atagagtgat
     2221 ttttttattg tgttttttcc ttcctttgaa ttatttatat ttttttcaat agaattattg
     2281 agtgtgctat tgttgtcctt tgacttattt cgaattacac gcttacgcgt atcacttgca
     2341 ttgacagagg taaccataag tgataaggaa agtagaatta aagatatttt catggactag
     2401 ctccgtaaat atttttgagt tttttagtgt gcgcgtatta tctagtattt attgtaaaag
     2461 atcaatactt aataaaacat taaaagagtc aaaaattttt tttattcaac gttatttttg
     2521 ggggattttc ttatgcatga gctgcaggga gcaatacacg atcaaacaac ccaaggctaa
     2581 atgttctgcg gatccttggt tgtttttcaa ttcattcgct tgtttaaaat gtgcctagtg
     2641 atcttttttc gtgaggctcg agaggatact aaagggattt tccttgacgg cctgtccttg
     2701 tttcggggct gaagaggtgt cttttcgagg gtaggggtcc aaagaaagtg ctaaatattg
     2761 ggcgataagc tcaccaatat cgatctttgt atccttaatg ttttcaatat cgtcattgct
     2821 gtcgtcatca taaaatccct cgaggtcgtc gtcttcttgg ggcgagaccc gaattttaat
     2881 attgacttgc tcctctatat attcgtcaac aggggatagg gtaatgacgc acgcttgggt
     2941 gacatgcgct ttgagatagc ctgtgacttt aaagcatcct tcttcttggt cttgacggat
     3001 agtaaattct gcggaaagat ttttgacatc aaggatttta aagcgcttcg caagggcctt
     3061 gcactcttca gcggttgcag caacggtttg cttttcagca atcgccgaag gaagggctaa
     3121 gtcataaatg cggctaaatt cgttgatcat tgtcaaaaat cattcctttt tttattgaac
     3181 tgcagggtcg atccacccaa tatgcccgtc cgagcggcga tacacaacat taagtccacc
     3241 gtgttttgca tttttaaaca tcataacgag ttgatccgaa aggtccatgc gcatcacagc
     3301 ttcgcccaca gtaagcgttg ggacgctact tgtcatttcc gcaatcacaa tgggtgtatc
     3361 ctcgccctta tcgtcctctt cggcattcac aatatattgc tgagctggaa tgagtatttc
     3421 ctcaatcatg gacgttttgc ggcgcttatc gcgaaggcgt gatttatagc gacgtatacg
     3481 gttttccatg cgttctcctg cttgttcgca ggccctatag ggatccgcat catcaccatg
     3541 tgtgcgcaca ataaaatgct tactaatatg aacagaaaaa tccacaataa aacgatgtgt
     3601 ttcttttgta acaacgatat gcgcctcgat gggatcacca aaatacttat tgctcattgc
     3661 attgatacta tcctcaatat gtttgcgcag tgagtcgccg acttcgatgt gttttcctgt
     3721 aatattaatc gccatgtgtt tctccttttt aacgttgtgc tgcattaact ctctattgct
     3781 aaaattttag catttttttg tccccattta tagtatttta gacgctccga ggcattttca
     3841 atgcgcaaaa catcgcggta ttttgcaacg gtgcgacgcg ccacagaaat tcccatgagt
     3901 ttaagtcttt caacaatgtc actatccgag aggggcatcg cctgattttc ctcgctaatg
     3961 attttgcgta tgagggattg aatgcttttt gcactgtgaa ggttatcatg ctcccgtgca
     4021 ttgacattca tactaaagaa atatttaagc tcgaaaagtc cgcgtggtgt ttcgatatat
     4081 ttatccgttg tcacacggct cacggtgctt tcgctaagat ccgtttcaag ggcaatttct
     4141 ttgagggtga ggggtttaat gctgcccatg ggagatctga aaaacgctgc ttgtttacga
     4201 atgatcgcat gggtcacttt cataatattc atggcgcgtt gatgcaaact tttaagcagc
     4261 cattttgctt ctgtgaattt ttcttggagg aagattttgt cattggtgga tttcatcatt
     4321 gatcgaagct caatataata agagctattg aggtacacct ttgggagatt gatgggatta
     4381 agttcgaggg accattcgtg gggatttctc catgccatca gaatatcggg gatgacttga
     4441 tggagtgtgc ttttccccat gctgagcccg ggtttggggt gaagtgtttt gagtgtgttc
     4501 agataatctg tacatgtttc taaggggata tgaagtttcc gtgcgaggtc gtttggcgag
     4561 ccagcgtggg atttaagggc gaggagaaag cgttcaattt cagggctgta aaggccacga
     4621 tattttaatt gtaatatgag gcactcctca agtgaccgtg caaaaacacc tgtgggttca
     4681 agggtttgaa ggcattctaa aatattttct attttattaa aattttcatg ccccaaaagg
     4741 atcatatctt cttcaaaaga gggaggaaga tacccctcat ccgttaaaag atcgcacaac
     4801 gcataagcca tctcacggtc ttttttatgg atgcgcagat caatttgatg gcggataaaa
     4861 cttttaaagg ggatgtcctc tgtcagatga agaatcggac tataggcatt atcggaagtg
     4921 gttgtggtaa gttgataatc atcctgccag acattttcat agctttgagt atcaaaagta
     4981 gacgtatcag aaaatgtgtc tgaaaaagcg tcacaataat tttcctgaag ggtttcctca
     5041 aagtcattag agtcttgtga atcatcatta ttattttcgc taaattcttc ggtttcaagg
     5101 agtggattcg attcaatata ctccgccaca aaagtgctga gctcctgatt ggacatctgc
     5161 aaaagcttaa tcgcttgctg cattttctgt gtcaagcgaa gctgaagact atttttgagg
     5221 tcgagtgtct gtactgtcat gcaagtagta tgccatgaca aatctttcca agaagtaaac
     5281 gccgcgtcga tttttggaag gtttttgtgc gagggaattt ttcttaattt ttagcgtgat
     5341 gagtatcctt agaggcatgt taaaaggaaa aatgatgcat gttaaagctg tggataaatt
     5401 cagaaaaaat aaatgaccag gtgaaatcta gagtgagcgg gtgaaatcta gagtgaaaat
     5461 ttttccccta aatacacgcg gcgcacattt tgatcggaaa taatcgtttt tttatcgcca
     5521 tgacaaagaa tttcaccacc gtggagaata tacgctctgt caacaatatc aagggtttcg
     5581 cggacattat gatctgtgat gagaatgcca atattgcgtt ctttgaggtg aaaaatcata
     5641 tcgcgtagtt cctgcatggc aataggatca atgcctgcaa ggggttcgtc gagaaggaga
     5701 aagtgaggat tagccgcaag ggcgcgtgca atttccacgc gacgacgctc gcctcctgag
     5761 agagcaagag agggggcata ccgcagatgt gcaatgtgaa actcatcaag aagtgtgttg
     5821 aggcgttctt cgcggtgagt tttatcgggg tcgacaattt caaggatcgc acggatatta
     5881 tcctctacat tcattccacg aaaaatagag gcttcttggg ggaggtatcc tatgccaaga
     5941 cgcgcacgct tatacatcga catc
//
LOCUS       DAIAHG010000066         5963 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_66_length_5963_cov_2.667686, whole genome shotgun sequence.
ACCESSION   DAIAHG010000066 DAIAHG010000000
VERSION     DAIAHG010000066.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66_length_5963_cov_2.667686"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1834 a         1339 c         1245 g         1545 t
ORIGIN      
        1 aatattggtc agcgctttat gattttgaca aatatgggat tacctgcgag ggtgtggata
       61 ttgatattca aaaaatgatg gggcacaaag atgcggttgt gaatgatctc acatctggta
      121 tcgcgttttt attcaaaaaa aataatatcc aatttattga ggggcatgcg tctcttaaaa
      181 aaaatcctga gggtgttgcc gtgcatgtga atgcatcctc gggggacgtg catcttttaa
      241 aagcacatca tattcttatt gcaacaggat cagaacctat tgagattcca ggacttcctt
      301 ttgatgagat aaaaattctt tcatccacag gggcgttaag ccttggggag ccgccccgcc
      361 atcttgtcgt cattggcggg ggttatatcg gccttgagct tggttctgtg tggaaacgcc
      421 ttggctcaga tgtaacggtc atagaggcaa cggatgatat tgtgatgacg atggatagtg
      481 acgtaagaga ggcgcttaaa aaatcgctga aaaatcttgg cataagtttt aagtttaacc
      541 gcaaaattgt gggctaccgc gcgcatgaag gaaagcagca cttacaactt ttagcaacca
      601 atgaaaccct tgaaaaagaa gagatgatga gttgtgatgc cgttcttgtg gcggtagggc
      661 gacgtccaca tactcaaaac cttggccttg aggaggcagg cgttgaaagt gatgatcgag
      721 gctttattcg tgtcaataga cactatcaaa caagcttacc aggcgtttac gccattggtg
      781 acgtcattgg tggaatgatg cttgcacaca aagcagagga ggaaggtatt gctgttgttg
      841 aatcgatgaa aggacaagcc ggtcacgtaa actatggtgt tattcctgcc gtgattttca
      901 cacagcctga ggtagcgagt gtagggttaa cagagactca agccaaagaa aaacacacgg
      961 taaaagtagg gaaatttccc atgacagcca atagtcttgc caaagccaca ggctacacag
     1021 aggggtttgt caaaatcatt gcagatgcga aaacagaccg tgttttaggg gttcactgcg
     1081 taggtgcgca ggcaggagcc atgattgcag aggctgcgat ggccatggaa tttggcgcct
     1141 cgtcagagga tattgcccgc acgtgccatg cccatccaac catgagcgaa gccatgaaag
     1201 aagcagccct agaaactttc tcaaaggcta ttcataagtg agaaaatagc caggagttca
     1261 gagtcgagga aaatttacca agattaaata gatatttgta cttttttaag agggatagaa
     1321 aatttgaatt tttcttcaac tgattttgaa atcgggcttt ttgcccccat tttacttatc
     1381 aatttttttt atacattttt tctaataatc tcgatttgtc tacaaaggcc aagaggataa
     1441 agaagctttt ttgtcgtagt ctctctcccg caagaaagtt aaaatttatt tttcttgttt
     1501 tttttgaatg attaaactat attaccctga agttagtctc cttcgtctag aggcctagga
     1561 caccgccctc tcacggcggc aacacgggtt cgaatcccgt aggagatgcc aaaattcgga
     1621 taatttgagt cggttatcct tctggaaaaa gaatcagaat aaaaatagct ataccgatca
     1681 caatggcgtg atgaaaaagc cgttctgctt cgcgcgtatt agacgcgtca aaccgccgaa
     1741 tatccttaaa aatatcaaaa ataatcccaa agacaagcgt tccataaaac catatgtccg
     1801 cccccaggta cacacctaaa aaagccatta gcatgttcat caccaaataa aaccctgcaa
     1861 gggcatagcg gagatggtgc ctaaaaaaaa cggctgtgga atgcacgcct atcaagagat
     1921 cttctttaag atcttgcacc gcatagattg tatcataata aagtgtccaa aaaataccaa
     1981 tcccataaag aatccatggc tgtggtttga gaagaagaaa aggttgaagc tgcgccacgg
     2041 ccacaagcgc accgatatta aaagcaaaac ctaagataag ttggggaaaa acaaaaaaac
     2101 gttttgtaaa agggtaaaga aaaagcagcg ccaatcccac taaagaaatt atctttgctg
     2161 ctgtatttaa aaagaaaaaa acaccaagcc ccccacttag aaaaaaaata aaagggatga
     2221 gtgcttcttt ttttgaaaga gcacctgcgg ctaagggtct ctcttttgta cgcgcaacct
     2281 tttgatcaat agtaatatcg cacaaatcat taaaaataca cccagcactc cgcatacaaa
     2341 gcgccccaaa cgcgaaaagc actccccact ttataaagtg cacataagga ggagagcaaa
     2401 gagcaattcc ccacaaacag ggaagataga tgaggagaat aggcaaaggc ttatgcaagc
     2461 gcgctaattt aaaatagtgg aaaagcttta gcatcatcgc ccctaagcct tgacaacttt
     2521 ctctctcaaa tctgatcggg tgacatggcg tgtcgcattg cgtgcatcga tcgttacagc
     2581 tgtgtgcttg acaatcagct cataatcaac gtgatcgtga tccgtaacaa ttacagccgc
     2641 atcgtacttt ttaagactat tttcggtaag atcaataccg atcattccca caagattcgg
     2701 gtgctcgcgc atgggcttga tttctggaat aaaggggtca tagtaatcca gattaacgcc
     2761 cctttcccgt aaatgctcca taatgagaaa tgcgggactt tcacggctat catccacatt
     2821 ttttttgtaa gcaacaccca caattaggat atgactcccc ttcagtgatt tttgaaaacg
     2881 gctatcaagc gcctttgtca aacggtccaa aacataaaaa ggcatacttg tattcacctc
     2941 acccgcaagc tcgataaaac gggtggaaag accaaattca cgggccttcc atgtcaaata
     3001 aaatggatca atcggaatgc aatgtccgcc gaggccaggc cctggataaa aaggcatata
     3061 gccaaaaggt tttgttttag cggcctcaat cacctcccac acatcgatcc ccatggcatc
     3121 aaagacaact ttaagctcat tcaccaaagc aatgttcaca gaacgaaaaa tattctccgt
     3181 aagtttaatg gcctccgctg tttcaagaga gcttaccggc actgtgtcaa tcccaaaact
     3241 atcgtaaacg gcttttgtca gcgcaaggct ttgtgcgtca agacccccta caacttttgg
     3301 aatttttatc gtgctaaatt gagcattccc cggatcctct cgctctggcg aataccccat
     3361 aaagaaatcc tcacccgccg tcaggcctgt gctctctaaa agaggcttca tgagttcatt
     3421 cgacgtacca ggatacgtgg ttgattcaag aataatcagc tgatctttgc gaagatgttg
     3481 cgcaatcatt tctgttgttt gcaccacata actcatatcg ggatctctat atttgctgag
     3541 tggtgtcggc acacacatca aaataacatc agcatccttc agttttgaaa aatcggacgt
     3601 cgcagaaaaa cgatcgttct cttgaagctc ctcaatagat tttttggaaa tatgatggat
     3661 ataggattcg cctctattga ggttcataat tttagatgga tcaatatcaa accctatgac
     3721 agaatgacca gctttgtgtg caacacagac aaggggtaag cctacatagc caagaccaat
     3781 cacagcaatc gttgcttttt tagcgtatat tctctcaaga aaaggaagct cctcctgaag
     3841 attaaatgca gaatttttat ttattttttt ttgcaacgca gctgtcatgt gtgtcagtag
     3901 attctcaaaa taggcatgag aacacataac cggattcaac acaagaggtc aagaaaattc
     3961 cctcattaac catcttttca cattactaat aatagttaat gacgaatcag ctgacctgat
     4021 gaaagaagaa ggtagagatg gtcattcaca acgataggct gtgacacaat accatctttg
     4081 agctggaatg atttgacctt tgtgccatcc gccatgcgca taaagacaag atcaccccct
     4141 gaggatccaa aaacaagttg atcattgacg gccacaggtg ccgtccacaa cagtgctttc
     4201 ttctcgtcag aaactggaag cggtgagacc caatgcactt tgccagagga tttgtcaagg
     4261 caaaccacgc ggttattatt atctaacaca aagagaacct ggcctataag cagtggggaa
     4321 cgaatactcc caatgtcttg ctgccagctt gtaaggcccg tcgaaagatc aaacgccgcc
     4381 atgcgtccac cgtggcttat aacatagaga atattgccat caacgatagg attacccaca
     4441 atatgtggaa tactcgagat tgaatcactt cgaagtgcgg tcgtcatcgt atctgaccaa
     4501 agcggaacgt cgctttctgc cttcatcaca aaaatttcac cggaagaata ggtggagaca
     4561 acaagatcat cttttacaac ggggctggcc cctccttgaa tacttgaaac ttctgtaaat
     4621 ccttgatggg tccataaaag ggttcctttc acagcatcaa gcgcagctgt catgccatta
     4681 atacctgtga caaatagacg gccgtctttt acagcgggca tggagcgtat aggagcgcca
     4741 aaggacgtgc gccattttat tttaacctga ccatccgcgg ccttcacagg atcaagggcg
     4801 ataatatccc caatagctgt ggcgacaaaa agagttccct cataaaaagc aagggtagca
     4861 catccagcac tatcacgcgc ttcttctgga atagagtcaa aggtccacaa gatttccgca
     4921 cctttatctt taccgaggtc cttcaccgcc gagatttttc caagggtatc agagacaaaa
     4981 atcacatgac catcactcac aggatggcct aaaaagcggt gctcgggcgt ttctccttcg
     5041 ccaatcgaga gagaccatac cggcgattca ctgagtgtcg caacatacgg cacatgctct
     5101 gtatcaaaat taatcagtgc ttgtggaaaa gctttaagag gccgtgcagc aggcacagaa
     5161 actttttttc ctttaaaatc aggatcagga aggagatcac cactcgtaaa atcaagatat
     5221 atttcgcgca cacctggaag tggcggtttt ttggaaaaac tatcacaagc ggttaaaaaa
     5281 agagcaagag agacggctaa aaacgcttta ttcatatgta ttaaaatccc ctgaaaaatt
     5341 aaggcttaaa ccgaacattt tgattcgaaa gctgcaccat catttgtgct cttatgcgaa
     5401 gcccttctgg agtattttca tccttcacaa gggcggcaaa aatctccaaa gcgtccttgt
     5461 aacggccgtc tgtgtagtaa attaaccctt taagttcctc cacaaaaaga cgccacggat
     5521 ttttagtatc actcaaaggc tttaaaagct ctaatgtttc ctccgaggat tttaattttt
     5581 gctcataact tatcttcgca aacaaaaacg ccgcataatc cttcacatac acgggcaagc
     5641 cactatcctt tgccattttt tctaaaagat cacaggcttc cttaacactc gccgcatccc
     5701 cttttgccaa aagagcggca cttttttcaa aatgacgtaa atagccataa acatcatgat
     5761 tactcgtccg catggcctca aaaacagcaa aggcctcggg caatttattt tgagcaatat
     5821 acatttgcgc tgttaaaaac gcatccgaca attccatatg ttttttctgc agatgatttt
     5881 gccataacat gtacgctgtc gctgctgcaa gaatgctcac agccgctgtc gttacatgcc
     5941 gaccatattt tttccaaagc tgg
//
LOCUS       DAIAHG010000067         5858 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_67_length_5858_cov_3.682408, whole genome shotgun sequence.
ACCESSION   DAIAHG010000067 DAIAHG010000000
VERSION     DAIAHG010000067.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67_length_5858_cov_3.682408"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1654 a         1177 c         1352 g         1675 t
ORIGIN      
        1 gctgaaaatc atttatcgat cttaagcagt gatacctttg atcaagagga tattaatgcg
       61 atttttcggt gcgtccactc tgtgaaaggg ggggcaggga cttttggctt tgacaccctt
      121 gtttccttta cgcatatctt tgaaacaaca cttgatattt tgcgcaatga tacgagtcat
      181 ttaaatcctg cccttgtcga tcttctttta cgcgcgaatg atattcttgt tgttcttgtg
      241 caaaatgctg aaaataatat cgacagccct ccagaggcca tggcgtctgt caaagaacag
      301 cttctcacct ttgtaggcag cacaacgggc actccgtctg cgccagcagc gagtcatcag
      361 ggggttgagg aaaatagtgg tctcaaaaaa tatacaatta acttcctgcc tgaacttgat
      421 cttatgcgct ttggaaatga gccctattta attttcagag agcttaaaaa attaggcatt
      481 gaggaaagtt accattgcga ggttcataag gagcgtcttc ctgagtcgct taatgactat
      541 aatcctgagc attgttattt gacatggacg attcatattg aaacagaagc ttctatcgac
      601 cgtgttaaag aatgttttga atttgttgag gatgattgca aacttgatat ccaaacaacg
      661 acatcttctg ttcaagaacc cttagcagaa aaacatattg cctcgggcga tggaaagcaa
      721 acacaagttg tgcaggcgct taatacacca aaaacacccc caacagcctc ttcgacgcca
      781 caaaatcaac aggctcaagc tcaaacagat cagcaaaatg ttcattctat tcgtgtagaa
      841 cttgatcgta ttgatcgcct cgtcaataca gttggtgaaa tggttattaa gcaagccatg
      901 ctccttgatg cggcgcaaac actcaaaaac acagccactg aaagtactta tgatagtatg
      961 actaagggct tggaggaatt gacacaacat acgcgtgacc ttcaagaaag tgtgatgtcc
     1021 atccgcgctc agcctgttaa agctgtcttt gcccgcatgc ctcgcctgat tcgtgagctt
     1081 gcaacgcaat taaataaaga agtccatatt gatatgtccg gtgaaaatac ggagatcgat
     1141 aagacagtta tcgagcgcct tggtgagcct ctaacccaca tgattcgcaa tgccgttgat
     1201 catgggattg agtcccccaa agaacgtatc gatgcaggca agccctcttt tggaacgatt
     1261 caactgagtg cagagcataa aagtgggcgt attgtgatta aaattattga tgatggacgc
     1321 gggattaatc gcgaaaaggt tctccaaaaa gccattaaac aaggcatctt aacggaagaa
     1381 tcagcacctt cgggtgagga aattgaccaa cttattttcc atcctggctt ttcaacggcg
     1441 gattctgtca cgaatatttc agggcgtggc gtcggtatgg atgtggtgaa aaaatctatc
     1501 caggctcttg gtggacgtat ttctatccaa tccgttgaag gacaagggtg tcagtttaat
     1561 cttctcctgc ctctcacgct cgctgttttg gatgggatga ttgttaaagc gggtgacgag
     1621 tgctatgtca tcccgttgat taatattctt gaaacccttc gcccgtcaaa aaaacaagtg
     1681 agcacacttg tgggcggcgt ggatattctt gacctcagag aaaaaaatat tccgcttatt
     1741 tatcttcacg atatttttag tttgtccaat gccacacgcg tctccacaga aggcattgtt
     1801 gtcgtccttg agacagaagg tggcgaaaat attggtctcg ttgttgatga aattgtgacg
     1861 cagcaacagg ttgttcttaa aagtttagag gctaattatg acccgatacc gggtatttct
     1921 gctgcgacta ttttaggaaa tggccgcgta tcgcttattt tagatgtgaa tatgctgcat
     1981 cgtatgaaaa ataattatca ttaaggagga tatctttatg gaagcgacag ctcaagtttt
     2041 agaaaaaaat actttagaag aacaaaacat ccctcagcag tttatcacgt ttacagtacg
     2101 tgggcgggaa tacgggatta atattatgtc aatccgcgaa attaaaggat ggacagcaac
     2161 gacaaccttg cccaattctc cctcgtatat gcgcggcgtg attaatttgc gtggaactgt
     2221 cgttcctatt atggacctta gtgagcgttt ccagatgggt gcaacgcaaa caggcaaaaa
     2281 ccatgttgtg atgattgtca atattagtag ccgcttgatg gggattctgg tcgatgctgt
     2341 gtctgatatt ttaacgatta atcaaacgga cattcgcgct gttcctcctg tagagggaag
     2401 taatcataca gagattttaa gcggccttgt gagccttgaa gatcgtatgg ttggtctcct
     2461 tattttagaa aagctatttg ataaaaatct aagttttatt gaaggatccc ctgctatgga
     2521 tgagtcgttt gcgacggcaa acacccaaat acaaggtgag ggtggtcttg gtactgatgc
     2581 aactgaagaa gaactttagg gaaatgtgag aaaaacccat gcaacatcat gcccatcatg
     2641 aggatgaagc tctagagaac gtcaaagaat ttccttttac agataaggat tttcaggcga
     2701 ttagtcagct tataacaggc ctcacaggca ttgttttaaa gtatgaaaaa aagacgcttg
     2761 tctactcaag attggctaag cgtttaagat ctcttaaaat gggctctttc tctcagtact
     2821 gtaaatacct tgagtctccc tcagggaaag gggaaataag ctttctcatt aatgcaatta
     2881 caacaaatct gacgcgtttt tttagagaag aacatcattt tcatcatatg cgcgatgttg
     2941 tgatcccaag tattgttgag cgctaccgaa aaaatcctac agctgagaac agacgcgtgc
     3001 gcttttggtc ggctggatgt tcttctggag aagaacccta ttcccttgcc atgacacttg
     3061 ctgcgcatat gcctgctgga tttgatgtga aaattcttgc cacagatatt gatacaaata
     3121 tgcttcaaaa aggaaaaaac ggtctctaca aggatgggga gggtataccc acagagtatc
     3181 ttaagcgctt ttgttcaaaa aatgctgatg ggacacttca aatggagaga aatatacgcg
     3241 atcttattat ctttaaccca ctcaatttac tccatcaatg gcctatgcat ggtccttttc
     3301 agtccatttt ttgtcgcaat gtcgtgattt attttaataa agaaacgcaa atggagattt
     3361 ttaataagat ggcagcactt ctcgatccaa aggggtggct ttttattggc cattcagaga
     3421 gtctctttaa agtctgcgat gccttcgtcc ttaaaggaaa aacaacctat acactgggca
     3481 cacaatcatg atgaaatcag ctgtaaaaac gagttttttt gatccaaaat ttcaagcaaa
     3541 ttgtgttaat gtcctgcctg gtgaatttta tagtacgcat gacgatctta tgattatgac
     3601 gatcctcggt tcttgcgttg cggcatgtat tcgcgataaa aagcttggca ttggagggct
     3661 caaccatttt cttcttgcag aaccccatca agaccttgcc tcaccaagta cacgctatgg
     3721 atcttatgcg atggaatgct tgattaatga tattttgcgc caaggaggct cccgtgaaaa
     3781 ccttgaggtc aaagtttttg gaggatctga tttgatgaat agtacgattc agattggtca
     3841 aaaaaattgt gaatttatta aaaactattt gcaaaaagag catattcata ttgcagcgga
     3901 ggacttaggg ggctcttatc ctcgtcgcat tcacttttgg ccccagtcgg ggcgtgttgt
     3961 ccgtcttatt ttacaatcca gtgaacgtag tcaagtgatc gagcaagaga aaaaatacac
     4021 aaacgttatt aagacaaagg aaaagagtca tggtgatatt gagctcttcg ggtagtgcac
     4081 aaaaaacaaa agttcttatc gtcgatgatt ctgcgctgat gcgtaaaatg ttgtcggaca
     4141 ttttagaaag cgatcctgca ctcgaagttg tgggacttgc ctctgatcct tttgttgcac
     4201 gcgatcgcat aaaagcgctg aatcctgatg tgattacact tgatattaat atgccccgta
     4261 tggacgggtt atctttttta gaaaaaatta tgacgctacg gccgatgcct gttgtgatga
     4321 tttcaacgct tacggaaaaa ggtgcgaaag aaacgcttga ggcgctcgag cttggtgcgg
     4381 ttgattatat tacaaagcct acaacggatc ttaaaaatag ttttgagcgt attcgcagtg
     4441 aaatttgtgc aaaggttaaa gtggcggcta aggcacgtgt taaaaatcgc cttgatcata
     4501 aagtttctgt gccaagtctg atcgcggagg ggcggcgtgg acttcctcaa cttgtggcga
     4561 ttggctcatc cacagggggt gttgaggcac tgacagaggt ttttagatct cttcctgccc
     4621 attgccctcc cgttgttgtg gtgcagcata tgcctgcagg atttacagcg agttttgccg
     4681 ctagactcaa caaacaatgc caacctactg tggttgaggt tcagccggga atgcgcattg
     4741 aaaaagggac aatttatatt gcaccggggg atcggcattt tcgccttaag cacgcgtcaa
     4801 gtgcaattgt ggcggacctt tatgacggtg aaacggtgag tggtcacaag ccctctgtgg
     4861 atgttttgtt tcactcagtt gcagcgctta aaatgcgtta tgttcagggg attattctga
     4921 caggaatggg gcgcgatggg gcgaagggtc tgctcgaatt acgccaaaat ggtgcacgga
     4981 cttttggtca aaatgagtct tcatgtcttg tgtatgggat gccgcgcgcc gctatggaac
     5041 ttggcagtgt ggagcaggag attcctctgc aaaaagtatc ttctgtgatt atggggcatg
     5101 tttaatacat agtcatttgt tcataaggaa aaatttcagg gtaagacact ttccaatagt
     5161 gatgattgtg ttcaatgatc aaggacgctt cttcaaaagt atgctataaa agatttatca
     5221 aaaataaaac atgcattcat gaacatgcaa aagccccatt ggatcaaggt taaagcaccg
     5281 acctcaaaag aatattttga aacactttcg ctcgtcaaag agcaaaagct ccacactgtt
     5341 tgcgaggaag cggcgtgtcc taatattggc gagtgctgga aaaaaaagca tgcgactttt
     5401 atgattttag gcgaagtatg cacgcgtgcc tgtgcttttt gtaatattaa aacggggcgt
     5461 cccgatcttt tggatcccca tgagccagag cgtgtaggcc aagctgtcgc acgtctgggg
     5521 cttcgtcatg ctgtaattac ctctgttgac agagatgatt tagaggatgg gggtgctgca
     5581 catttcgccc aaacaattca cgctatcagg cgtttttctc cctccacaac cattgaaatt
     5641 ctgacccccg attttttgcg caaagaagaa catattccaa cggttgttgc agccgctccc
     5701 gatgttttta atcataatct tgaaacagtg ccacgccttt atcccacggt gcgtccggga
     5761 gcgcgttatt ttaatagcct ctcactgctg aagagagtga aggaaattga tcacactatt
     5821 tttacaaaat ccggtcttat ggtaggactt ggcgagga
//
LOCUS       DAIAHG010000068         5852 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_68_length_5852_cov_3.698182, whole genome shotgun sequence.
ACCESSION   DAIAHG010000068 DAIAHG010000000
VERSION     DAIAHG010000068.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68_length_5852_cov_3.698182"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1651 a         1187 c         1239 g         1775 t
ORIGIN      
        1 gtctttcgaa aattttcttt tcaatcgatc ttgagacgca ataaacaaaa gaaagtttca
       61 caatggatat atcaaaagct ctgattatcg acgattcgcc ggatgatgcg tgtctttatg
      121 agcgcttatt gcagaaggta ggccctgtgt cgattgcgca ctatgcgtca gctgaggagg
      181 ctttgggggc tctttcaaaa gaaaatcacc cttttgatat tatgttgttg gattaccata
      241 tgcctggcat gaatggcatg gattttttaa gaaaattgca tcaagaaaac atcatacttg
      301 atgctcccat tattgttttg acagggcagg gcgatgagag cgttgctgtg aattttatgc
      361 agcttaatgt ctcagactat atcaaaaagg atggtttaac ggcgaatatt ttgaaaaaat
      421 caattcaaaa agcgcgtgct tctcatcgac aaaaaaaaag ggagcaggca aaacagaagg
      481 agcttttgct gtttgcgcat acattggcgc acgatcttaa aaaccccata gggcgtattc
      541 gctcttatgc gatgctggct caaaaaaaac ctcaaaaaag cgaggcttac atcaattata
      601 ttaccgaaga tgcggcgttt ctcgtcgaat ttattgataa attgttagtc tatgcagagt
      661 ctgggcgtag ctttgaggac agagaacctg ttgatcttat ggagattata aaaaaatccg
      721 tggccctttt ggaaatgcct attcaggaaa aaaaagcgat gattcatctt ccaacgtctt
      781 ttccgcctat ttatgggtca aaagtggccc ttgtgcagct ctttcaaaat attttatcca
      841 atgcgctgaa gtactgcctt gtgacacctg aagtttttgt ggaggcattg atggaaaaaa
      901 atacagttac tctttcgatc agggataatg gtattggtat tcccaaaagt gtcagtaaaa
      961 agatttttga ccctttctac cgtgttccga acaagtccga agcagagggg acaggccttg
     1021 gtctagcgat tgtgaaagca attgttgatc aacacggcgg gcaaattgac gtgatgcgtc
     1081 ctaaaaaagg gggaagccag tttaatatca ctctccccct tttgatccat tagtgagccc
     1141 tttctggaag aaggcaggtg tttaaccaat agtcttggat agaatgacag atatttttca
     1201 tttggtaaaa attcacaggt ttttgaatat acgcactgac accgtgctca tagcaatact
     1261 gaacatcttt gtgatccgaa gatgttgtca aaacaatgac aggaatgccc cttagttgtt
     1321 tattgttttt gatatacttg agaaaagatc gaccgtccat cccaggaaga ttgagatcaa
     1381 gaaggataaa aagagggatg tcttcttcat tttcaattaa agaggcaagt gcatcatccg
     1441 aatccttaaa ccatttaagg gggtataaac aatcacagga ctgaaaccca cgaataatag
     1501 cttcgcagtc atctttattg tcttcaatga gcataatatg ttgtgtttct gtcatctttt
     1561 aatccttgtg gcagtgtaaa ataaaaagtt gttccttgcc ccatttcgga ctgtacccaa
     1621 atagagccat gatggcgatc aataattttt ttcacaaagg tcaggccaac gcccgttcct
     1681 tttgtggcgt cgtcttctgt attcaaacgt ttaaagatgc gaaaaatatc gtgataaaag
     1741 cactctttaa tgccaattcc gttatcgcgc acaaaaaacg tattgttttc catggatcca
     1801 atttcaattt ttttaagagg tttatcgttg tattttattg catttgtgat caagtttcga
     1861 aaaacttctc gaatgcgtat gatgtcgcat gtgaccgttg gcaaaggatt aacaatctta
     1921 acctccacag aaccttcgac aatcacagca tctaacatcg atgttacatc atgaacaata
     1981 tcctcaagcg gtgcctcact gatggctaaa tcctgctttc caaggcggga gaaataaagc
     2041 aagtcgttgg tgagttgttc catgcgacct gcaagataaa tcatacgatc aatgcgtttt
     2101 atcgcatctt cattgagctt attcccccaa tcttctttta aaaaaagagc attattacta
     2161 agtcctcgaa gcggttcctt aagatcatga gaggcgatat acgcaaagtc atctaactct
     2221 tggttgcttt gtttaagggc tgttatatat ctttcaatag aagcttctga ggcttttcgc
     2281 tctgtgatgt ctcgcacggt gcccacgaac atcaccatat catttacatt catttcatta
     2341 acgccaaggt ccatcggaaa aatctcacca ttttttctcc gtcccttaac ctcgcgacct
     2401 ataccaataa cttttggcgt gtgcgattct aagtagtttg taagatagct atcgtgagcg
     2461 ctttggtagg gctccggcat caacatttta atgtttttcc caagaacttc attaggttga
     2521 tacccaaaga tacgtacagc agagggatta aatgcgtgaa taatgcctaa ataatcaata
     2581 ataataagac catccaaaac agtgttcatc acagcagtta agctaatgtt ttggaagaca
     2641 tcgtttttta tattggacgt tgacatgagc ctaagtcgct gttctccaga tatttaagaa
     2701 aatataataa aaatttttta aaaaagatat attttttcaa aaaacccgag catctttttc
     2761 tttaaaacag agtgttatga atctatgcgg ttaatacatc gctaataatt tttttgtgta
     2821 aaaaagcctt ttgagtttcc gtatcgtcga gctggcacaa ctcaaatgcc gctttccaaa
     2881 gggcccaggc cttagctctg agccatgtat tgttatccaa actgagggac tgtttaaaga
     2941 tttcacgcga ttttcctttg aaaaaagtcc atgccatcac aagatcacac gccggatcgc
     3001 caatccccat ccccccaaaa tcaatcacac cagtcagttt tccttcctga agaagaatat
     3061 ttccataagc catatcgcca tgaatccaaa caggttcttt tttccactgt gttgccatgg
     3121 cgttttccca taaatcaagc gcttgatcgc tgttgataac cttagaaagt gcagcgatat
     3181 atgtgcgtgc ctcggcatca taaacacgga tatgatcgcc gcgccaccaa ttatgaaggc
     3241 ccggtgttgg cccatgggat ggatcaatgc gatggagttc tcttaggaat tgtgcaaggt
     3301 cataggcgat tgattctaac aaattatcct ctaccgcggt aaattcattg gcactttttc
     3361 cggggagcca ctgataaatg gaccagggcc atggataata tttagagggt tttcccataa
     3421 aaagagggct tgggatcgaa agggataaat gcggggcgag gaagggtaac agctcttgtt
     3481 cttttgggac tttaagggcg taagattctg ctgtggggag gcggatagac atctcttcgc
     3541 ccaggcgaaa tgtgcgattg tcatgtcctt gacgtgcgac aggctgaaga gtgaggcgtg
     3601 caaactttgg aaattgttct tggataagtt cttgtgcaag ttgtgttgta atgttgagcg
     3661 aatctgatgt cataagggcc tcgagtgtat gcagttttac tatatggatt tatattatat
     3721 atgagcatat tatatatgaa tttactatcc tgctctcctg catgtgagga ttatatctgg
     3781 gctttgcttt gggtcaaaag aagtttggtc aaaatcgcca tagacacgga taatgctgag
     3841 tccctcgcta tggagaagat cttttaaagc atcaaaggct gtaaaacgca gcgtgagcga
     3901 tgtgcgtgtg gtgtgatctt ttccaattct ttgtgtttcg tagaacataa gatgattttt
     3961 tggcgtatac gtttgccttc catagacgcg aatatcctca ccgctgggat gtgtaaatgt
     4021 gtgccaaaac tcaaaatcct ttgttgtttt aagatccgcc tgcctgggat tgcgggtgca
     4081 aaaagcaaac acgccgtctt ctttcagatg tgtcttgaca caatacagca tctccttttg
     4141 ctctttttct gtgagaaggg cttgaaaact attgcctgcc agggtgataa ggtcaaatgg
     4201 attgtcgaaa ctaaaatgct ttatatccat ttttgcataa tgaatcttta cattggggga
     4261 caagtgggcg gattttttct tagcgtgcga gagcatcttt tcattttcat caatgcccgt
     4321 acagagcaat cctttttggg ccagggcaat ggtgagtcgc cccgttccac aggcgagatc
     4381 aagggccctg ccgtggcttt taaggccgca aaaaaaatta aaatcgccgg tccagccgcc
     4441 atattctgca tcataaatcg ctgggtcaaa atattcgtgc tgaggctgca taattagcgc
     4501 tttgaacgct caatttcttt tttacggatg gccatcattt catcgcgggg accataaaaa
     4561 tctccctctg tgtaaaggcg cgagcacaaa aggccattca cgtgatcgat ctcatgctgc
     4621 aatagccgtg ccagaaaacc gtgggcaact tctttaataa aatgtccctc ttcgtcatag
     4681 cctgtgtaaa gaatacttcg tggccgataa acttttccgc ccaaatcaag gactgaaaag
     4741 caaaattccc agtcataggt ggtatctgaa tcctctatga cctcgtacgt agggtttatg
     4801 agcgctgtga gcggtacaac gtctcgtgca tcctcgcgaa tagccaaagc ttctttggtc
     4861 acatgataaa caatcacgcg gacatcagca cccacttgag gtgctgcgag tcccgctcct
     4921 tgcaattcta tgagcaaatc tttcataccg ctgatcaggt ttttaacgtc gtgagaaaga
     4981 gggaacgtca catctttagc gggggtgtga aggacctgcg ctttgtcttt attgcatgtg
     5041 gtgacaatgg aaagtgtcat agagtaatcc taaaaaaatt ttgtaacttt ttaggattat
     5101 aaagtgtttt agctcaaagt aaataagcgt cagcgggcgc tagtccttta tttcattcaa
     5161 aatcagctca accgcatttt ctggaatatt acacgcttta aacgcgtttg acgcatcatg
     5221 ggggctcgct gcctcaagag ctttttcaaa ggatttttga tcaaagcact gaggtacttt
     5281 cttatcctca ataaggaaat tagtgtactg atttttttga gcatcgagat aaatcacggc
     5341 atatccgcag aaataccctt gcactcccgc tgtagagcgc tgcgtgacta gaataggatc
     5401 ctttaacgcg tgaacaagtt tttctgtttt aaaatccttg ctattgatga cttgaatatg
     5461 tgtgaggtcc ttgacaattt ctttttcaaa ggcaccatcc actttggggt gaatgctgat
     5521 aaaaacatta acatccttgt ccttgatggc attgataaac aaacgaaagg aatcgttata
     5581 tccattgtta ttattttcat aattcccaag atacaaaagc gtttttcttt ttttcgcacc
     5641 ttcttcgcgc catgtgttca gtgttggttg acccacaaca cgaatgattt gtttaggttt
     5701 gcgcttttga agtgcgtctt taaccgcctg actgggcaca agaatcgttt gattatttgt
     5761 atcaagcgcg tcaaggatct tttcaaaatc accgccaagc acactaaaac tatcgtaata
     5821 cgccttgttt ttatgtttac acgaaagatt aa
//
LOCUS       DAIAHG010000069         5844 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_69_length_5844_cov_3.136293, whole genome shotgun sequence.
ACCESSION   DAIAHG010000069 DAIAHG010000000
VERSION     DAIAHG010000069.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69_length_5844_cov_3.136293"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1710 a         1078 c         1298 g         1758 t
ORIGIN      
        1 ttgtttttgt tttttttcat gttcctttct tttccttctt tgggaatgat tattgaatgc
       61 gattttaatg aatatttttt tggagaaaat aaaactgttt ctataaaagt tgttcaagaa
      121 attcaaccag cggcagaatt ctctgaagtt acctataacc actctttttt cgaaatcact
      181 caaaaatcaa aaacagtatc tttttatgtg gatgaagaaa gtgactggaa agttgaaatt
      241 tttgaaaaga ataacttgct tgaaaaatat tcaaatctca aggggagtag cgatcctttg
      301 acactaaagt ttttgggcac acacaccctt aaattttgga ctgttcttga agagatgtag
      361 atttgctaag cgcgttactt ttcacaacaa caaagaaagt atgttaagat aatacgtatt
      421 ctttttcttg gctgttttta aacggaacga actatggcct ttgccgattt gaacaaacgc
      481 tcccttgata tttttagaaa tcttgtggat gcgtatattg agacgggtga acctgtgggc
      541 tcgcgcacaa tctcaaggcg tatggaggat tcgctttccc ccgcaacgat ccgtaatatt
      601 atggcggact tagaggatgc agggcttctt tatgcgccgc atacttctgc aggacgtctt
      661 cctacagatg cgggtttgca ttttttcgcg caagggcttt tggaggttgg tgatctcagt
      721 gcgcatgacc gagaaaaagt agaagaagcg tgctctgcct ctggaaaaac tattgaggat
      781 attctggagc gtgcgacgac tctattgtcg ggtttgtcca aatgtgcaag tattatcctt
      841 gcgccaaaaa tagaatctcc tcttaagcaa attgattttg tgccacttgg cgggcaaaaa
      901 gtccttgttg ttctcattac cgaagatggt cttgttgaaa atcgtattat tgaacttgaa
      961 gcgccactgt ctatatcgac gctcaaagaa gcagggcttt atatgacaga caagcttatg
     1021 ggcaaaaccc ttttcgaggc taaaaatttt atcaaaaaag aactcagcct ttatcaagag
     1081 caactggata cgctgacgtc aaaagttgtg gaagagggct tggccgtgtg gtctgggggg
     1141 gataagacac gtaatttaat cgtcaaagga caaagcaact tattgcaaaa tgtcacgatg
     1201 ctggaggaaa ttgatcaaat tcgggctctt tttgatgtgc tcgaggctaa ggagcatttt
     1261 attaatcttt tggatgcagc gattcatgcg cagggggtgc aaatttttgt aggggctcag
     1321 catgatcttt ttaaactttc ggggtgttca atgattgttg ctccctataa aaatacacag
     1381 caacaaatta ttggtgcgat cggtgtcgtg ggccctacgc ggatgaacta cgggaaagtc
     1441 atatcgcttg tggattatac agccaaggtg attagcaaaa ttcttggggg agattaaagg
     1501 tttttcgcct ttataaggtt aaaaaaatag ttaatatctt ttgatccagc ttaggccaac
     1561 gccgccgctt ttgttggtag agacatcaaa atcgaggctc caattgtcgc tcagttgttt
     1621 ttcaataata gccttacttt ctttgctttc ttcatctgca gcactaatgg cctgttcgat
     1681 gacgacacta aaatccggtg agatttgttt gcgaatgcta agggcctggg gcgattcgct
     1741 ggggcttgtc tgatctttat ttttaatttc aagcgcatca atgccaaaaa gtttacgaaa
     1801 ggttgtgaaa aaacttttat cctgtccagc actggaaaga ctggagagag aggcactcac
     1861 atcaaataat tgaccaaggg atgtggtggc tgatttgcgg ccaaaaagaa tcagcgcgag
     1921 gatttcctct tggcttaagc ccggccgtga gataagggta aaccgcgtat ctttgggtct
     1981 gccatagagc tgaataaaaa cctcgtatcc gtcaatcttt ttcataatct caaggtcaag
     2041 gtgaggatta ttaattttat gatcaaagat gatttttccg cgcgtaaaat tgagaatgcg
     2101 cccagtaata tcgacattgc ctttttctaa agtaaattca ccaacaaaat cgggggtgtt
     2161 tccttggagg ctaaggccgc cattccacgt gctttcaatg ccaaatccgc ggaccacaac
     2221 aggctttgtc ggcacaagac gaatatcaaa atgaaatcca gaatcagaag gctgtgctgt
     2281 gatatcatca tttttaagcg cgccatctgt aaaataaata cgcggtgttg cagacggtgt
     2341 tagctgatca atatcaacga cgacaggatt aagattcaaa aggcctttga tggttttttt
     2401 gaagagatca atgaaaaggt gcccatccgc acgccctgta aaaagatcgc tttgccctaa
     2461 atagaattgc tttagctcaa gatcaatcac gcccgaagat tgcatatcaa aaggaatctt
     2521 cccagtagca ttcaatgttc ctttaaaatc atcttttccc tgaagatcaa ggctcgcgtg
     2581 gtcttttaaa agtttgatcg tgccctgaag gtttttaata tgggttccaa tggcaaaatt
     2641 ttgatacaaa ccatttttaa tcacgatgtt cccatcgaaa tggggggata gaagtggtcc
     2701 tgacagggca aaatcccctt tgaaggctcc accaaggtga tctcctttgg gcagaaaaga
     2761 tttaagataa gaaaaattac cttctgtgga ggctttaatc tgaagatttt ctggcgttgc
     2821 tgtgcctttg attttgagat ttaatccttg tttttcttgt agcgcaccct cgagagaaag
     2881 aatgtgatgt tgataaacgg ctttgatatt ggcctgaaga aatgagaggt gtttgaaatt
     2941 tttaatattg acgggcgtgg aggagagctt tgcttggata tgtggattgt caaaagatcc
     3001 actgatatcg gcggtaaggg taagtgcggc gttactttct gcaaagggga ggtctttttt
     3061 caaaagatca aaggggatat gcgcactcat ttttcctttc agggtgtcct gaaaacttag
     3121 actgtctaat gtgaatttcg cgctgcctac aaaaaaatta gtttctttgg cgattttttt
     3181 ttcatcaagg gcgatagaaa aaggtttttc atttttgagc cagagagact cacttatttg
     3241 aaaatgaata gtattgatca tgaggttatt aaaatgcttc aaaaaacctt ctgcttgacc
     3301 cttgtcactg tgaagcgaaa aaggaatatt ttgaaaatct tttatccctg ctttgatctc
     3361 tagggggatt ttatcataat gcgtttggat gatcagtttt tcatctttta aggcgagagt
     3421 gccgctgttt ttaaaatttt ctttttgaaa gagataatca gctttgaatg tatggatcag
     3481 attcatcgca ttgaaaggag gggaaaggaa ccccagagca cgcacatcga gcaacacgga
     3541 tgtgagataa atttctcccc aggcggcgtt ttttatcttt acatttttaa gctcgagatt
     3601 cagaggaaaa aaaccatcca cacgctcgca ttcaatcaca gagtgtggaa gagcgcgttc
     3661 cagtgaaaaa agaataatgt cttttttata gatattgagc gcgccaaaca gagcaaaaag
     3721 aacaatacca atccaaagag aaagacgaag aatgacggta tgcagcatgt taaaaattca
     3781 tcaaagagtc atcatagttt tttaaatatg tttttcttaa accgtttatt cttttataga
     3841 acctatgatt ttaaagagaa aggacttatt tgccttaacg ctaggaaatc tgtgtttggc
     3901 ttagcttttc tataatatta ttgattaacc tatggatatc ctcacaccga tggggaaaag
     3961 gcttatgatt ttcttttctc agaatatcca gtaatgacac aagattgagg aggtgtatgg
     4021 tgctatgcca attttctggt aaagattttc cattacatgt gagtccgcgg atgaatgaat
     4081 cttcaaaatc gggtggcatt tgagcgctat agcgaagcat attcgcgaca tcccacaagg
     4141 gagacccaga aaaagagaac tcccagtcta aaatgccttt gaccttccat gtatgatcag
     4201 tttggtcaac caagatattg gcgggatcaa aatctccatg gacgaggcaa ttttcatttt
     4261 tatcaggaaa taaatgtcca aaggtgttga ggcacgtttt gatgctgaga attgtttgct
     4321 gatcaagaac cgcgagaaca ttatttttgt ctaaacactc ccttgcaaag gtggataaat
     4381 catctgaatt tttgggagga ataattgcaa ggtttttatc aaaaaaaccc gctgttggaa
     4441 aggtgttttt gtgtatctgc gaaagtaaga cgccaatatc aaacatgatg gcgcctctat
     4501 catggggcaa atcgctgagc aaaagatcac gcagtgggat accgcgtata aattctgtga
     4561 gagcaaaagt gtagtgatca atgtttccga taaaataaaa agtaggcagg ggaagtgtat
     4621 tttttaagag gatactaaga tttttttcgc gaaaagcggc ctctttgtcc cacagataca
     4681 cacgtaaaat aaatggattc tcaacacctt caaggttgat tttgatatta agattcgcac
     4741 agcctccttc tatgagttgg tgtgagataa gcttttgacc tgggcaggca aggtgcacca
     4801 ttctctcgat aagattgtgt gctaagggat gagacgtact cgttttttcc caatgaattt
     4861 taaacattta aatcttttac ccctatgaag tctttaaaac gcttggccca cactaatata
     4921 aaattggaaa ggggcatcga tctttttgcc tttggatgag cttgggtcgc ggcggcgttt
     4981 aaaaggaacg ctaatatcaa aacgaatggg acctatgcct gtaaaatagc ggatcccaat
     5041 accaccactc cacaaaagat tactgctatt gagttttgga atacgttgaa gccctacgct
     5101 tcctgcttca ataaaggccg cagcgcctac tttttctgaa agcggaaaac gtgcttctgt
     5161 gccaaattct agaacactca acccaccgat aggtgtggca tttgaatcaa gagggcctaa
     5221 gagttgatag ccatagccgc ggactgagtt accaccaccg ctataaaagc gtttgttggg
     5281 aggaataaat tgaacatttt cttgaccata aatcacgccc acttttcccc atgtggcaag
     5341 aacaaaaata ttgtcagcct cctcaatcgt ttggatttta ggatgaaagt atgtgcttgc
     5401 aaaaatttca ttaagccaaa aaccattatg cccaatgtat tttcctcgat aaggatttgt
     5461 tttgatacgg catttcagtc cttcgacggg gttgagaaga ctatttgtgg agtcataatc
     5521 taaaataagg ggtacgccca aaaggttgct gcgggtgcga ataccattgc gcttaatgcg
     5581 actgacttca taatcaaggc cataggaaaa ttgcaaaaag ggcgtggcat cataggagag
     5641 tccccctgca acttttgctg attttgagtg gtaggggcga tttttttctt ctgtgagact
     5701 gagagcagaa taaagagtct gtcttttaag gataaaatcg ggtttagaaa aagcaagttt
     5761 tgcctcattt tttcgccgtc cgagcgtgag ttttgtctca agagattcac ctttgcccaa
     5821 aagattatca tgccgccaaa aaat
//
LOCUS       DAIAHG010000070         5826 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_70_length_5826_cov_2.776657, whole genome shotgun sequence.
ACCESSION   DAIAHG010000070 DAIAHG010000000
VERSION     DAIAHG010000070.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70_length_5826_cov_2.776657"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1665 a         1153 c         1255 g         1753 t
ORIGIN      
        1 gaacgtttcc atgtcaaaat cgatattgat gacgaggggg gggtgaccat agcgggtatg
       61 accaaagaaa aattgaacgc agctgtgaca gcgatcaaag gaatgtttga ggagcctgag
      121 attggtaaaa tctataaggg aaccgttaca aaagtggttg attttggggc gtttgtgaaa
      181 ttccttggcg agcgtgaagg gcttgtccat gtgtcggaaa tcacaggcga acgcatcgag
      241 cgtgtgtctg atgttttgca caatggccag gctgttgatg tgaagttcgt tggttttgac
      301 gaccgtggca aggtcaaact ttccatgcgt ggtttgttca aacagtcgta aaatagagtc
      361 tcagaccttg tgatcatgca tcgccgggtc tgagaattac tctgattatt ctatagtatc
      421 attgttatca tcgttattgt tatcatcttc gtcatcttct tttttcatat ttttagagac
      481 gatctctatc gctttatctg tgattctatc gtgaatgtcc ttcatttttt catactcact
      541 ttttgaaatt ttaaaatgat cataaacctc gttcattact tcattgagca cttgaggaat
      601 gataacgtta ttgatcctgt ctgtaatacg ctcatattgt gacttgagaa acgcactttc
      661 ttcctccttc gacattcttg gattgctttg tgtgtaaccc tctttttcgg agtcttgcca
      721 agcatcgaag agctcctttc tctgtgtatg tgctccttgt aagtgtgtgt cattatcaaa
      781 taatgtatcc tcaaaatcta tgatttcttt aaattttttt atcttttcaa gctcttcctt
      841 tgtggcgatt tcctttattt tctcctcgaa ttttttcatg aaatgtaatt caagtttatc
      901 ttcttcactg gcccaagcac ttgagagtgt tgtacttacc ccccccccca agcacaaaac
      961 aagcgataat gcaattattt tttgacgtat agacatcata atctccttta atgttaaaat
     1021 tacaaaatta attctttcaa gaaaaagtta atattacgta aaaaataata tttatgcatc
     1081 acgcatgtat aatcaaaggc ttaaaatcct cttaacaaaa aaagtcaaag agtgttaagg
     1141 tcaaagaaaa ataaaatgtt gtgacgatgc acactcttta tatttgcaca acctgtaaaa
     1201 aacgcgatgg cgatgatatc gttgacgcac acgctggcga aaaattttat gcagctgtca
     1261 aagaatcttt tgatgtgtgg gcgcataaaa atgattttga acttgtccca acggcatgtt
     1321 tgagtggatg taaaaaatcg tgtacttttg cgcttcaaaa agaagggaag tatgcctaca
     1381 tttttggttg tgcagacgag ggtatgatcc aagatatttt taatttagcc gcagcctatc
     1441 aaagcaacgc gcagggaatt ttaaaaaaaa tcgaccgccc tgactgtttg aaggataaag
     1501 tgctggcgcg catcccacca ctcaggtcat aatagagggc atgagcttta taaaaacatc
     1561 gctattggca tccccccaag atcttccgaa agtttctctt tctggaaaga aatgggattt
     1621 ttttgagtgt gatgaaggag atatcgggcg ccttgtcaca gatcatggtc tttctgaggt
     1681 cgttgcaaaa gcgcttcttc aacgcaatgt ttcaacgcaa acagcacgcg attttttaga
     1741 tccaaagata agaaatcttc tgccgaatcc cctttgctta aaggatatgg agaagggaac
     1801 ggatgtttgc tttcaagcga ttgaggcggg tgagaagatt gctatttttg gcgattatga
     1861 cgtggatggt gcaacgagct cagcgctctt gcggcggtac tttcgggcca ttggtcttgc
     1921 aacagaactt tatattccag accgtttggg cgaggggtat ggtccaaatg ctgaggcgat
     1981 ggaaaatctc tatgctcaag gtattcgtct tgtcattatg gtagactgcg gcacgcttgc
     2041 gtttgaacct cttcaaagag cccatgatct tggtcttaag gtgattgtac tcgatcatca
     2101 tttgagtgaa gaaaatctcc ccaaagcgtc cgcgcttatt aatcctaatc gtttggatga
     2161 gagcgttgag cccttttatc taaaagaact ttgtgcagca ggcattgctt ttttatttat
     2221 cgtggctttg caaaaaaaac tgcgtgatca tcattttttt aaaacgcaag aagaaccgaa
     2281 ccttcttgaa tttcttgatc ttgtggccct tggtactgtc tgtgatgtca tgccgttgac
     2341 gcaccttaac cgtgcttttg tgacccatgg tcttcgtctt atggcgcgtc agagcaacgc
     2401 gggtattcat gctttgatgg aggtggcggg aagttctatg attccaacag cctatcattt
     2461 gggatttgtg attggcccac gtattaatgc aggtgggcgt gtcggtgagt cttcgcttgg
     2521 gagccagctt cttttttcag aagatcctgt aggcattaaa aatatgagtc ttaagctcca
     2581 caccttaaac acagagcgcc aaaaccttga aaagctgatg cttgatcaag cctatgccat
     2641 ggttgaagcg gagaaacttt atgaaaaacc ggttatttta atcggtcacg agaattggca
     2701 tccgggtgtt attggtatcg cggcaagccg tcttaaggag cgctataaca agccctctct
     2761 cgtggtgtct tatctgatgg atgcgacgat tgggaaggga tcagggcgct cgattgcagg
     2821 cgcttctctt ggtgaagcaa tgattaaggc tgtaaaaaag gggctccttg ttcacggggg
     2881 agggcatgct atggctgcag gctttacggt tctgcgcaag gattttgatg cgttttatga
     2941 ttttataggc gacaccctta aggacgctgt ggaggcctat acgcctacac atcaagtaac
     3001 aggatggctt agccttgcgt cttttacgcc atcttttgtc aaatctttga atgtcttaga
     3061 gccgtttggt catggaaatc ctatgccgaa atttgcgatt ggccctgttt ttattcgttt
     3121 tgccgatcct tttggtgtag atcatatgcg ccttgtcatc gaagatcact acgggaatcg
     3181 ctccaaagcc gtgcatttta ggggattatc acaaccttat ggtgatcttc tgatgaagcg
     3241 tcagggatct ttttatcttt tgggatcgct taaaatcgac agctggaacg gtcatgatga
     3301 cgtgagtttc gtcatcgagg atatcgcagc ggcaactgtg taaaagaaag agagatcatg
     3361 ttgatccccc ttttaatttt aggatattaa tttgaaccat tcatttgata ggtgaatgta
     3421 atcaaaaagc ggttatcaag tattttagac tctgcagatg ctttaccagc gcccgaagca
     3481 gcatttaatc ctgtgatttc gacttttccc aaaggcacaa gagagtactc agcgcctata
     3541 tgaaaacggt cacctgctgc ataacgaaag ccagctcctg ggacgatttg cctatagctt
     3601 ttattcacat tttgcgacgt gcgtgtgtta ttgatactta aaaattcctg atatttatag
     3661 gcaactgttg aaaattcata ggctaccttt gcatacacca taattttagg attaataagt
     3721 ttgccaataa taaacgcagg ggcataagct accttatggt gaatatcaac aatcccttgg
     3781 taaacatgat tggcaagata aatagatttc ttcatattat tgaaatattt tgcgacataa
     3841 agttcccccc ccaacatgat ggtggaaatg cctttttcag tgatataccc aaaatggaaa
     3901 gaagtcagcc ctccaaagtg agaaaacttt gattctgaag gtggctgaag aggatcaagg
     3961 tatttatgat ctcctttgat ccaacttgcg ccaaggccga ggcttccata aaaacctgaa
     4021 aaataaagac ttgcctgggc gacgtgcgag ctagcgagaa gaaacgttgc gaggagaatg
     4081 ctttttaatt ttaacatttt agataaactc tcttaaaaat agtttaacct aggattatag
     4141 tgaatcagat ctcccgtcaa ttaggaattt atgcaacagt gcgcaaggaa aaaaaaacac
     4201 gctttctttt gttgacacgc cccctaaagg atagtctttt gctggcgaag catttaaaaa
     4261 aaaggggcgt aatgacgctt cttcagccgc ttttatgtat tgaagaaacc ccctttgttt
     4321 gcccgaatct tgaggggaaa gagcttattt ttacatcgca aaatgctctg tatgccactg
     4381 tgaaccttat gcataaccgc acattaacgg tatggtgtgt cggtgaaaaa acagcagaaa
     4441 cggcgcgtga aagcggattt atcaatattc acacagccca aggaaatgga gaacatctct
     4501 ctctcatgat tgaagcggat cgaagaaata atcctctaaa aagacctcac tatattcatt
     4561 tttcgggcga agatatcacc attgattttg agaaaaaatt tccaggtgag tgccaacgca
     4621 tcattgtcta tcgcacacaa cctgcagaga cactacgccc ctccgtgcat cgtgctttga
     4681 ggagtcaggc tatcctaggc gtggttcttt attcaaaaaa aacggctctt acccttaaaa
     4741 atcttgttga tttacctcca tctcttccta tttttgcgct tagtgaggcg attgcatctc
     4801 tttttccaaa tcatccaacg tcgcttttta aaaacgagga tgatatagtg gctatggtta
     4861 aaaattctct taagacatag agtgcgatga cagatataaa agaaaaaacc aaggaaaatg
     4921 aacccgccga acctaagaag acagcggcac cccctaaaaa tatgttaaaa aataaacctt
     4981 ttttttcctg ggctaaactc gctgtcgtga gcattttagg cgtgcaaaac tatggtcttt
     5041 ttattctgca tcaaaaaata aacctccttg agggaaaatt aaaccatcac caaaaaacgt
     5101 attttattga gaaagaaaaa tttgacgcac ttgacacgaa gctaaaaaat cttgaaaaac
     5161 aaattttagc gatgcctaaa gaaaatcccg aggcgtcaaa agatcaacaa tcttttattc
     5221 gggatagtct tcaaaatttt tctttgagtg aaaaacaact cagcgatatt tttgagagca
     5281 ttaaaaatgc ttttgttctg aaatccgatt taaaagaagt gcaacaaagc aagctcacac
     5341 cgcttctcta tatggatgtt ctcaaacaag ctgtgatgag cggaaaacct tatgataaaa
     5401 ttttggagac cacacgcacg cttgtgctta gtaacacggc agtgaagcca gaaatcgtca
     5461 cagcttttga tattcttaag ctttctgcgg caacgggact gccaagcctt tctcttcttt
     5521 atcaattgcc agaaaataat cctgtcacat cgatgcattt accttcgtgg cttgattgga
     5581 tcaaggatta tatgaaaatc actgcggacg tttcaaaagg tctttcctca gaggcacgca
     5641 ggcgtttgat ggagatgatt gaatccgggc gtctggcgga ggcttatcag actgtgaatc
     5701 gtcatatcga agaacatagc gatcaaaaaa acctttggga gccgtggctc aaaacgatgg
     5761 atgtgcgtct taaaacgctt caaagcatcg atcttattca acaaaatctt ttggaaagcg
     5821 cagtat
//
LOCUS       DAIAHG010000071         5598 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_71_length_5598_cov_3.291433, whole genome shotgun sequence.
ACCESSION   DAIAHG010000071 DAIAHG010000000
VERSION     DAIAHG010000071.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71_length_5598_cov_3.291433"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1738 a         1073 c         1253 g         1534 t
ORIGIN      
        1 tttcggtgtg tttacaatgg gagggcaaaa agaagaggct tttgatcttg agaatcagat
       61 tgataacaaa aataaattca ttgtcaataa taaagatgaa gaagaaaaaa atgagaaaga
      121 agaaaaaaaa tatgatctca aaaatgttga agaaaatatt ataaaaattg aaagtatgaa
      181 aaaaatagac agaagaaata atagtgatat tgaggatagt cttaagaagg gccctctttt
      241 ttcttcccgc tatcgtttga ttattacagc caatgatatt gggaaagaca gtaccttttt
      301 gagctcgcaa tattttagta agcgaaagaa aacaagtgag ccttttgatg aaaagaaaaa
      361 ttaaaccttt tttgtgattt aatacggaag agtgccaagg gatatttgct aaaaatgtgt
      421 gattcaccta cactagggac ttagcaattt atttttaaat tttggcaaac cgtttgaaaa
      481 tttttatagc agcgggagag gtttcgggtg atattttagg ggcccagctg attgaggaga
      541 tcaaaaggcg ccacccttta tgtgagatta ttggggtggg gggacctaaa atgcttgaag
      601 caggtcttag caaaagtgtg tttcccatgg aggatcttgc tgttattgga ctttttgaga
      661 tactgcctaa gcttggtctt tttctgagat atcttttcaa gaccaagcaa ttcattcttt
      721 cagaaaagcc cgatcttatt attaccatcg atgcttcggc attttatctg cgcttgttaa
      781 aggcactgcg caaaaaaatg agtattccga cgattcatta taatgcgccc gctgtgtggg
      841 caagccgtcc ggggcgcgcg aaggaaatgg cctcttacgt ggatcatctc ctatgcctct
      901 atccttttga accgccgtat tttacggtgc atgggatgca ggcgacattt atcggccatc
      961 ccttgtgttc gcgagagggg gactcttcaa acatttcaga tatcaatgga aatccccccc
     1021 cggtttttga aaaaaaaaga acaaacgtga cggtgctctt tggcagccgc gcacaggaaa
     1081 tcgaaaagct tgccgatccc tttattcacg cctgcgtgct tcttcaaaaa gatgtgcccg
     1141 atttgcatgt gcttgtgccc acatttgagc ggtataaaaa gcaattaaaa gccaaactcg
     1201 atgcttcttc cttggcctat gagtttgtgg aggcaaaaga taaagagaaa gcgttcaacc
     1261 tttccaaagc agcattagcg gcaagtggta cggtcgcgct tgagcttgcg cgggcggaag
     1321 ttccttttgt gattgggtat aaactttcgc ccttgacgta tttttttgca aagcgtcttg
     1381 tgataacgcc ttatgtgtgt cttgtgaatg ttcttttaaa agcgccgatt attgaggaat
     1441 gcctccaaga aaaatgcacg ccagaagttc tttatcaggc gatgaaaaaa cttatttttt
     1501 tgagtccaaa ggatcataaa atacttttag acaaactgcg cgaatcctat catatgctct
     1561 gtttaaaggg aaaaaatcca acgcatacag cctgtgatat tatcgataag gttatgtgtg
     1621 acggtgtcct ccctcggcgt gaagaagaag agaaatactg aggaaaagac ctataaaaac
     1681 gggaaaccac gtggccaaaa gaggaggaag gcgtccgccg agaccaagag cgtaaataaa
     1741 gtctgtcatg aaatgaaaaa aaagccccat caaaaggcac atgctaatca aaaaactcgc
     1801 gcgcgatgag cgaacgggcc gaaggcaaaa acctgctgcg aggagaacca ttaccatcat
     1861 gagtccaatt tgcgcgataa gcttataata gtgcatgcgg taggggaggg tcgaaagacc
     1921 tgatttctct aaaagacgaa taaagtgagg ggtttcccaa aaagaaaggg tttcggggac
     1981 agcgttacta tcgcggatca ttttgagggt cagatccgta tgaagaaata ggttgtcagt
     2041 cctatgatta ttttcctcaa cattttttaa aaaccagtgg tgtgagtgaa tcgacgcctc
     2101 atcggcatca atccgacgga ggaattgtcc gtcatgacta aaaatataaa aggtaacatt
     2161 tttaaaaact cccttatgca ggtcaggggc tttgctgtga aaaatacggt cctcattcac
     2221 gtcaatttct ttcaaccata atcctgcact attgaggctc acagtacttt ttttccgtcc
     2281 aaaaagcttt tcttcaacaa aataaagacg ttgggttagg gttgtgcgaa tgggatttat
     2341 caacgtgata ttgatgatgc cgagcaaaaa aacacttgcg ataaggccat ataaaagatg
     2401 ccacaacgag acgcctgctg tacgcatggc ggtaatttct gagtgttggt tgagttttga
     2461 aagcgttaac attgcagagg ataaaataat aaaagggagc atgcgctcaa gctctcctgg
     2521 cagctgacac aaaacaactt caatggcatg aagaaacgta acatggggac gcgtcatcat
     2581 gcgcctaaaa agttcaatca tatcgaggag aagcacaatt gtactgacgc ccaaaaggac
     2641 agcgccaaac cactttaaaa aaagcgtgac aacatagcga ttgaggcgag aaaaaagata
     2701 aatcattttt ttacttttct caaaaaatct aggattttta tatcatataa tcgaaaaaga
     2761 agcgcactaa agcataaaat ataaaagcca taggccgtga caatagcttc ttgaaatgtg
     2821 tgattgaact gcaggcaagc gatcatcagt gcatgaaaaa gaaagccaca gacaaaactg
     2881 ggatgtaagc ataaaattaa gtccaatcaa tacataaata aaggcaaaaa agggttgcaa
     2941 aatgcgcaca tgggcggcag ctgtataacg tgtttgcgta ttttgatcaa gggtatcatc
     3001 gagggtcaaa agctcagtga tacttttttc gtaaggtcgt atgtgacgtg tttccttttt
     3061 ttgtgtcaga tccgacaaat catagttgag ggtttcaaat gttaactctt gaagctttcc
     3121 cgtttttgga tctctttcct gacgaaagcc gtggctcaaa atgaggaaaa ggtgcccgtc
     3181 aatagtcgtc attcgtcctt ctttggcaaa gagtgagtaa ggtttattcc cctttgtatt
     3241 gtaaagaaaa agtccctcca ttcggccact gcgatagcgt tgtttgatat aaatggtggc
     3301 gccgtggatt gtattaaacc gtccttcatt gagcatagcg cctgaaaatt gttggcgaac
     3361 ctgatgctcc atatccttaa attgcttaaa agaggggggc aaaatgtata cattaatgat
     3421 aaaggttagg gccacaatca atgctgttaa aaacaaaaca ggcgaggcaa tttgcatgtg
     3481 gctcaggcct gtgctccgcc aaattgtgag ctcatggtcc gtttgaaatt tatgaaagaa
     3541 aaacagaaca ctaatgaaga ggcaaatagg taaaatcaca acgagcaagt cggggataag
     3601 gaatcctaca agataaaaat accccttgag agacacatta cgatttacaa tcatatccaa
     3661 aaagcgcagc gactgtgtta gccacacagc aatcacaagg atgcttgtca gcaaaaatgt
     3721 ggcatagctg agttttttaa aaatatagcg agagactaaa agcataagag aaagtgtact
     3781 gcgtcttgca tcattcttcg agaaaaatac gtgcgattgt gtgaaaaaaa taaagagagg
     3841 gagaaacacc cctctctatt ctattttaga ttttactcat aaactttcgc tgcttttttc
     3901 aaaatagcag ctgaaagagg aatcccaagg gtatctgcgg tttttttatt aagataaagc
     3961 tcaggcttat caggacctgt ggcaggaatc gttgcagctg tgcgtttgtc cgcatctcgt
     4021 tttaaaagac tgcccaccat ttcacccgct ttaaggccaa tggcaaattg atctgcgccg
     4081 acggctgcaa gggcgccttt ttcgacaagc tctgtatcac tgacaaaaac agggattttg
     4141 tgttcacgtc ccacttttaa aatcgcgtca aaagcagcaa gggcgatgtt atcattgttg
     4201 acaaaaagtg cgtcgacttt gcccactaaa ctcatagcag caccgtacac atcactggtt
     4261 ttcgtggcat tagcaaaaac aagcgtaatg ccaaaatttg aggcctcctt cttcatggct
     4321 tcattcagaa cgatggaatt ttgctctccc gcactgtaaa taatacctat tttttgaagg
     4381 ccgggcaaaa cagctttaaa ggtttcaaat tgaactttgg gtgcgataaa atcattcacg
     4441 cccgagatat tgccttggag cttcgcattt ttcggatcag taacagaggt gtaaataata
     4501 ggtacattga tactgccatt ttgagctgtc tgttgtgtca cctgaagcgc tgattgcgag
     4561 gccagtgtgc cgatcgtgac aatgacatca taggatttcc caacgaactt ttgagcaatt
     4621 tgagaggctg ttgccgcatt gccttgagca gactcccact ttattatgca cgtgttattc
     4681 gctgtaaaac cttgattttc aagggctttg atcactccca gacgggctga gtcaagggct
     4741 tcgtggtcgc ttgtttgaat aataccaacg ctcatatctt tcttttctga gcaggcaaga
     4801 agtgtgaaaa gagtgaaaga aataaaaact aattttttaa aaaacataac caacctcgac
     4861 tatcgataga gtaataaaaa ttttttataa agtttagggg atgatggtga tgatgcataa
     4921 tcttgctcct tttaatgttg aatagtggtt tttgagtgcc ccatgacaag tgcttagtca
     4981 tgaatagtaa acgtcgcctt gcggacaaga tcgctaggaa gggatatgcc aagagctttt
     5041 gctgttttga gattaaggac aagctctatt tttttaggtt tttgcacacc aatttcgctg
     5101 ataggtttat gatcaatgag atattccaca ataagttcgc ctgttttacg gccaatatca
     5161 tactgattat aggcgacaga accgagggct ccccgtgcaa cagactcagg atccgctgta
     5221 aagacagcca ttttgtagcg atttgcaaca gtgatcacac tctcgaaagc agaaatgacg
     5281 gtattatcat ttggaatata aatcacattc gtttttccgg caaagttagc cacagccgtg
     5341 ctcacatcat tagtgttagc aactgtcgag cgtatcaccg tgagccccat gctattcgct
     5401 tctttttcaa aaagatcaac aagggcaagg gaattcgctt cacctgcatt ataaagcacg
     5461 ccgatggttt gaaggtctgg gaagattttt ttaattaaaa ggacttgatc gtgaacaggt
     5521 gaaagatctc tcacgcccgc aaccaaagga ttaggggccg atggatcata gagtttagcc
     5581 gcttttgggt cgctcaca
//
LOCUS       DAIAHG010000072         5560 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_72_length_5560_cov_3.476564, whole genome shotgun sequence.
ACCESSION   DAIAHG010000072 DAIAHG010000000
VERSION     DAIAHG010000072.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72_length_5560_cov_3.476564"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1607 a          995 c         1258 g         1700 t
ORIGIN      
        1 ggcgtatatt ttgacaaagg acgaaggcgg tcgccacaca cctttcttta gcaactatcg
       61 tcctcagttc tatttccgta caacagacgt aacaggaacg attgagcttc caagcggcgt
      121 tgagatggtt atgcctggtg ataatatcag catgaaggtt gagctcatcg cgcctatcgc
      181 gatggatgaa ggtctccgct ttgcgatccg tgaaggtggt cgtactgttg gtgcaggtgt
      241 tgttgctaag atcattgctt agttctgaac cttacaaaaa aagaaagggc gctcattggg
      301 cgcctttttt ttaatctata ccactctggc tctctctttt ttccctaaat actttttcaa
      361 ttttttcata gtatgtgtca gatatttcat cctcatgaaa acctatgcgc tgatcttgga
      421 taaaagcttt ttcgattgaa cttttttcta tgaaggaagt gatcaccttc caatcttttt
      481 cttctaaata ataatagtca aaagtaattt cctttaatgt gctgtgctta gaaatctcgt
      541 gccacaattc ctcatagaaa tagcattctc cgccaagata ctgctcaaaa gttatttttt
      601 ccaaggctaa tgtattaaga agtttaagat acaaatccgc ctgctcatca cgatgcaaaa
      661 cacccatatg aaaggttttg atattttttt catctttaat cacgtcataa agaacgtcat
      721 aagcatcttg cgtcagatag ggaagattca aagacagtgt gtgagcactt atgatatctt
      781 ttttaaagat atatttacag ttcacacttc tttgggaata ttctcttaga tcaaaatcct
      841 taagtttgag ctctgcgaca gtctctcttg cggccctaag aggttgagta agtgccaaaa
      901 acgccacagc gaggaagaaa atttttttca cgatgatctc tttattttga ttgagcacat
      961 catttttcac acacattcta tgcagagtaa acttttattc agagacttaa agggttctaa
     1021 agctatcctc aaaagcccat agaaaggggg cttattcccc cccttttttt ataatacatt
     1081 ccacactagt gaaagacttt ctcaataagt ttttttaaac tctgccgagc attattttta
     1141 ttgatcagta tttttatttt cttttgaaca tccccttcgg ggcctgtgca cacttgtgta
     1201 aaatctctgt gttcaaaaga aatacctgta atatttttat tttttctgag tgtgctaatg
     1261 atcgcttgtg tgtgctcgct tttttcagga taagtgctcc atgagccaac gataagagta
     1321 ttgagtgttt gatttttctc taagcttttc cacaatgtga tgattgcatt atttcttttt
     1381 tcatttgtgt catcttcacg aggggcttcc tcatataaat aatttaagtg cagcgtatcc
     1441 agctgcatcg tattgataac ctgagacagg tattttataa aatgcatatc attgcacgta
     1501 taatcaaaat caaaacattt taaaattttg ttgtttttta ggtgtaaaaa tagtttttta
     1561 tacaggtttt tatcaatgct ttttattcgc aatgtgctaa tattattttt gttaatcgca
     1621 tgaaaaagcc ggtcgagctg attttcattt ttgatcgaaa agtcttgcat tcctgattgt
     1681 tcaacaaata cttttaggga ttgggagtcc tcgtcctcca tttcatcttt gagagacgca
     1741 aaggtttgat ggtgataaaa gataattgcg ataaggggga aaaaggattt tatattcata
     1801 atatctatac cttttttgta aatttaagca tttacgctgt atacatatat gtctaaagag
     1861 ttaattctaa gttaatgatt tatgatattt ataatgaatt tctctaaact tctttaagtt
     1921 tagtggtgaa aatgctcttc tcttttaatt atcctagcgg gggaggtttt tttatgcact
     1981 gactgctttt tatgcgccca tttaaagatt tttcctctag acaagctgtg cgaataaggc
     2041 tataaaaaca cagctgtagt gtcggcgttt tttaggagtg tagctcaatt ggctagagcg
     2101 tcggtctcca aaaccgaagg ttgggggttc gagaccctcc actcctgcca ggtgtaagct
     2161 gcacagcatg ggttaggtaa gggatcaatg gtcgagaaga aggcaaatat aatcaagcaa
     2221 acagggaaat ttatccccga ggttcgtgaa gaggtgcgca aagtcacgtg gccttcacgc
     2281 cgagagacaa tgatgacaac cgtttttgtg tttgtccttg ctgtgattgc agccatttat
     2341 tttatgtttg tggatacaat tatccataaa ctcattaatg gcatcatcga ttacgcttct
     2401 cgctaatgca gatttttctt taaaggattg ttcgtttcta tgtcgcaaaa gcctcagcaa
     2461 aaagctcttt ggtatgttct tcaggtctat gccggatttg aaaaaaaggt ggcgcagtcc
     2521 attcgggaga cagcggagcg taaggggctt ttggattatt ttgaagagat tttagtcccc
     2581 tctgaggaag ttgttgagat taagcgcggt gttaaaacca ctgttgaaaa gaattatttt
     2641 ccaggatatg tccttgtcaa aatgcatttg acggacgaag cctggcacct tgtgcgcaat
     2701 acacccaaag tatccactgt tttaggcaat aacggcaagc ctttcccaat ttcagaagca
     2761 gaagttggcc gcattatgaa gcaggtgcag gattctgttg aaaaaccacg tcacacaatg
     2821 acgttccaag tgggtgagca agtgcgtgtg agtgatggtc cgtttacgtc ctttaatggt
     2881 cttgtcgaag aagttgatgc ggacaaatcc cgcttaaaag tatccgtgat gatttttggt
     2941 cgtccgacac ccgttgattt ggaattcacg caagtggaga aaatataaaa aagattttaa
     3001 caagttttag cccgaggtgg gctaaaagtg tgggagataa ttatgatggc tctaaaaatt
     3061 attatggaga aatttatcat gaaagtcgga ccactaactc agcaaagagt tgttttataa
     3121 ttaactgagg atattcatat ggcaaaaaaa attacaggct atatcaagct gcaagtgtca
     3181 gcgggtaaag ctaacccctc accacctatt ggtccagccc ttggtcaacg tggtttgaac
     3241 attatggagt tttgtaaggc atttaatgcg caaacgcaaa gcattgagcc tggcacaccg
     3301 attcctgttg tgatctctgt gtatgcagat cgcacgtttt cctttgtgat gaagacaccg
     3361 cccaatagct ttttcttgaa aaaggcagcg aaacttcaaa aaggaagcca aacacctggt
     3421 cgtgggacag ttggaaaagt aacgatggac caaattcgtg aaattgctca aacaaagatg
     3481 aaagatctta atgcgcatga tttggatgct gcatgcacga tggtgattgg ttctgcccgt
     3541 tcaatgggct tggaagtggt ggagtaaatc atggctggaa aaaaacttgt aaatgcgtat
     3601 aaagcgtttg atcgtgaaaa aatctattcc ttagctgaag cgctcaaaat tgttaaagcg
     3661 catgccaaag caaaatttga tgaatccatt gaaattgcga tgaatcttaa tgtggatgcg
     3721 cgcaaagctg agcaaaatgt gcgcggtgtt gtgcagcttc ctcatggcac aggtaaaacg
     3781 taccgtgtgg ctgtttttgc aaaaggtcct aaggccgatg aggcgaaagc tgcgggtgcg
     3841 gatattattg ggtctgatga tctcgttgag atgattcaaa agggcgaaat gaactttgat
     3901 cgctgtatcg cgacacctga tatgatggtg catgtgggta aagtgggtaa aatccttggt
     3961 ccacgtggct tgatgcccaa tccaaaactt ggaacggtga caatggatgt tgctggtgct
     4021 gttaaagctg ttaaaggtgg tcaagttgaa taccgcacag aaaaagccgg cgtgattcat
     4081 gcaggcgtgg gtaaagtaag cttttcagaa gaaaaactcg ttgaaaatat cgaagcgttc
     4141 atgggggctg tgcaaaaagc gcgcccagcg ggtgttaagg gaacatacgt taaacgtatg
     4201 agcctgagct ctaccatggg ggttggtgta aagtttgagg cacctgctgc ctagtgataa
     4261 aaaactagtg cgcaacgtgc tttattgaaa tgctgtccga gattgtgggt ctttttgtaa
     4321 aaataaaaag ataatggggc caaaagagtg gctccgcctg catgagacag aaggacataa
     4381 aactccttct gggacagatg acctaacatc gggtggttcc ggtgaagggt ttttggttta
     4441 atcaaccgaa ctgttaggag tgtaaaatat ggaccgttca caaaaaacag aactggtcgc
     4501 acagatgaaa gaaaagctca cagagtcttc tcttgtggtg gtcacccatc aaatgggtct
     4561 cactgtgggg gaggcaacgg agcttcgtcg tcaagtccgt gcaacaagtg ctgaattcaa
     4621 agtactaaaa aacacattgg cgcaaattgc tgttcaagag acacctcttg caggattaga
     4681 gcctttgctt aaggggccaa cggcattagc tttttcaaag gatccgcttg cggcagccaa
     4741 agctgttgtg gggtttgcta agaaaaatga tcgccttaag gtcattggtg gtttttttga
     4801 tggccaagtg attaatgcgc aaggtgttca aaagctggcc gagcttccat cgcttgatga
     4861 aatcagggct caaattcttt gtgttatcaa tgcaccagcg acaaagcttg cgcttgtaac
     4921 aaaattgtat gcagaaaaaa gacaagccga agaagcttag tttttgatct gttaaatgta
     4981 tttttagtat taaaaaaaat tagaaggagt ttttaaaatg gcaaatctcg ataaaatcgt
     5041 cgaagagctt tcaaaattaa caatcatcga agcagctgac cttgtcaaaa agcttgagga
     5101 gcattggggt gttgtggccg ctgcagctgt agcggctgca ccgggtgctg ctggtgctgg
     5161 tgaagctgtt gaagcacaaa cagaatttga tgttattctt gctgatgcag gtgcgaataa
     5221 aatcaatgcg attaaagaag ttcgtgcaat cacaggcttg ggtcttaaag aagcgaaaga
     5281 ccttgttgag ggcgcaccaa agcccgttaa aacaggcgtt agcaaagacg aagcggataa
     5341 gattaaaaaa cagcttgaag acgctggtgc aaaggttgaa attaaataag attcagcttt
     5401 tggagacttt ttatgaggca acaaaatggt cgttgtttaa aacaacaagg ctttttgttg
     5461 ccttttttcg agagcacttt ttttgtaata tgatggtttt tcatattatt aactaggtgc
     5521 tttttttatt ttacaattga aagagggaat atttagatga
//
LOCUS       DAIAHG010000073         5448 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_73_length_5448_cov_3.569168, whole genome shotgun sequence.
ACCESSION   DAIAHG010000073 DAIAHG010000000
VERSION     DAIAHG010000073.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73_length_5448_cov_3.569168"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1572 a         1229 c         1164 g         1483 t
ORIGIN      
        1 aatttggtgc aggaaaagcg cccacaaaat cttaaaaaaa taagattatg acactctctt
       61 aatctttgag acctggggtt gaagctgatg ttttgcaaaa cgtctatagc ttccctcttg
      121 ctccataagc tctgcatggg ttccaacggc ttcaatctta ccatcatgca tcacaacaat
      181 acgatcacac tgaagcaccg ttgtaagact gtgcgcaatc acaagggttg tgcgcgtact
      241 cataaaatca taaatacttt tttgtacaag ggcctcgttc tctgcatcca gcgcatttgt
      301 ggcttcgtcc aacaataata ctgttggatt gtgcaaaatg gcgcgcgcca aagccacacg
      361 ctgtttttga cctgcagaaa ggcgggtacc ctttgtgcca atatacgtct tgaggccatg
      421 aggtaatttt gcaatgacat catccaaacg ggcgcaatga atcgcgtaat taatctctgc
      481 aggtgttgca tccggctgcg cataaagaat attttcatac aatgtcgctg agaaaagagg
      541 cgcatcatgc cccaccatcg caaaatagcg gcgcacttcg tctgatttaa gctctttaag
      601 attaagccct tcgataaaaa tagaaccgct ttgaggcgca taaaatttca tcaacatcga
      661 aaaaatagtg cttttccccg agcccgacag ccccacaatt gctatttttt ccccgggtgc
      721 tacggataac gtaaaattct caacaaccgg tttctgcgga tgcgttggat acgcaaacgt
      781 cacattatga atggcaataa tgccccttgg attagaagga agtgctcggg tgccatccac
      841 acgcttaggg agtgagagca aggagagcaa gcgctctgcc gcgccaacgg tacgctgcag
      901 atcaccataa agctcgctaa aagaccccgc agaactcgcc gcaaggacag cataaaaaag
      961 aaaagcagaa agagcaccgg ggctaagcac accatcaata acatcttgtc ccccgcacca
     1021 aagtacaaag ttcacaccac aaaaagctaa aagcatcaca agacacacaa gtgtcgaacg
     1081 cgctaaaata cgcttaaccg taatttgaaa ggattctttg cttaactctc ttaggtactg
     1141 ggcttcccgg gcttcatggc catagccttg aacagtttta atatgactaa aggtctcctc
     1201 aaaaaaccca ctaatgtgcg caagacaacg ctgattttcc tttgagtaat ggcggatttt
     1261 attcccaaac gtcaaaaggg gaatcagaat aaatggaata aggacaaggg tatagagggt
     1321 aagttttggt gatgttaaaa acatcatgac aatgccaccg atcatcagca aaaaattacg
     1381 catcgccaca gaaaagcttg cgcccacaat cagctgcatc aaattagtat ccgttgtcag
     1441 tcttgctgtg agctcgcctg gacgattttc atcaaaaaaa ctcacatcca tatgaagcaa
     1501 gtgctcaaac actttgcgcc gcaacgatcc aatgacagcc tccccaagcc acgacacaag
     1561 atagctccgc aaataactgc aaaaagcaat aaaaagcgtt gttccagtca gaataaggag
     1621 cgcccaatca agacgcctgc ggtcccctgt aatcaggccc tcatcaataa actgacgaat
     1681 cccccatcca gcggcaagaa caacaaacgc tgtcaaaaaa agggtcaaaa tcgctgcgat
     1741 aacgcggcct ttatgctgct tcaacggttc aaagagaatg gaaaaagccg caagacgaag
     1801 ctgtttggga gcaggcggag cttttttaag tcggtgatgg cgcatgggta accgtttttc
     1861 tgagagcaga agaatctatt ggtggagcca ggggggatcg aacccccgac ctctacaatg
     1921 ccattgtagc gctctcccag ctgagctatg accccgactc tcccttttta tcggttcctt
     1981 tggctaaatg caaggatgtt gtaagaagga atgatcacgt cattcagaaa attgactcaa
     2041 ttaacgtaat cataaatcta agattttatt attcatacac agagcactag ccctaaaaac
     2101 gttgccgtga tagagtaagc ataagcatga aagaaaaaag atcactctca ttacgtttct
     2161 ttttcgcact tttttttacc gccttactct cgctattgga ttatttttgt cagcgtgcag
     2221 caaggaggat tcgccatcgt ccaaaattct ccttggcgag aatggtttat tttatgatgt
     2281 tttggagctt attcacacac attacgttga tacagtgaat gtcaacaaac tcgttgcagg
     2341 tgcattaaac ggcgctctta acagccttga cgatttttca aattattatt ccgccaaaga
     2401 ttataaaacc ctgatggaag ttctgaatgg tcaatttggc ggcattggtg cggaaattaa
     2461 aatcataaaa gaaggtgttg agattattgc cccccttgac gatggtcctg cctttaaagc
     2521 gggccttaag caaggcgata ttattactga attggatggc aaagaactta aaagactttc
     2581 gccagtagaa attattcaag ccgtgcatgg cgctcctggc acgacgctta cgcttacact
     2641 tttgagagaa ggcaagcccc ttaaggatat caaaataaca cgtgaaatga tcacagacgt
     2701 tcctatcaaa ggtcagctgg acggtcgcgt tgcgtatatt cgcttgagtg tttttaacaa
     2761 tcaatcggcc gagcacctta aaaaaacgct ggatgatctg aacaaaaagt tacatgaaac
     2821 ctttggcaac gccatgacct ttcaaggtgt gattttagat ctccgtaata atccaggagg
     2881 cacactcgat caggctgtgc ttgtgaccag tcttttcctt gacaaaggaa gtgcgattgt
     2941 caatgttaca gagaaagaac cgtcttctca tcgcacattt tacgctcaag ggacagatat
     3001 cctcaaggat attcccatcg ttgttctgat taataaagga tcagcctcgg cgagtgaagt
     3061 tcttgcaggg gcactgcgtg atcataaacg cgcgattctt gtgggtgaaa aaacctatgg
     3121 caagggctct gtccagcaga tttttgatct cggcgcaaag ggtgctgtga agttaaccat
     3181 tgctcatttc accactccag acggtcataa aattcatgga aaagggcttg agcctgatat
     3241 tttagttgcc tcagaaccaa cagaaacacc tatcaaagaa ccctcatcac gcgataaaga
     3301 tctcaaaccc gtggaccatc aaaagcaaag agcactcgat ctgatccaag gtctttcggc
     3361 gcttaataag tggtagagac gaaagaaaat acgcatgttt aataagttga aaaaaatctt
     3421 tgcgaaaaaa tcacggccta ccgaaaataa aaaaacacaa actgcccctc gtattgctgt
     3481 tgtgggggca ggacttgcgg ggttgacagc agcctggcga ttgcatcaaa aagggcatga
     3541 tgttcaggtg tatgaggccc gccctcgtgt gggtggacgg gtgcatactg tattgattaa
     3601 aaatcttaca ggagggtatt cccttgcaga gctggggggt cagaacatgt atgacggcgg
     3661 cgatgcgctc tctattcaag cgcttgcaaa agatctgggc gttgaacttt ctgcagatac
     3721 aagcgatttt tcacgtcttt ttgtggatgg gaaaaaagtt tacgaccctc atttactcct
     3781 ccagcaatgc catttcacac gccagacaat tgctcaaaaa ctcgcgtcac tcaaggactc
     3841 ggcacgccat atgggcgatg ttttagatgc tttttttgag gggaattcta ttctcaagcg
     3901 tctttttacc tttcaacttg caggctatga gggatctgca cctgaaagat tagcccttca
     3961 tcataatctt gaaacgttta ttcatatgct gttgggaggc ctttctgcgg ctgcacaggt
     4021 cgaaaataat ttgaaaatta aacggcttat ggcacgggat ggaaatgcgc atattccgct
     4081 tgcgcttgaa aaaaaactcc aaggtcgcct tcatctgaat aaaattcttc aaagcgttat
     4141 ctcggttgat gaaaaaatag aattaacgtt tagtgacggc gaaaaagttc tttatgacaa
     4201 agttatttta gccattcctt gtcctgttta taaggcgatt gactttgacc ctcatgtcct
     4261 gccgcccgat gttctcagcg cccttctcaa agtacaatat ggttcgaatg ggaaaattat
     4321 gatgcccgca catcatcaaa atcgctgtta tggcgccgtc ataacggatg agatggtcgc
     4381 ttttgataat gtcgatggtg aaattttaac actttatttt acagaaaaaa gtgctgaaac
     4441 actcaatcaa aaagcgaaaa cctctgtcaa aaaagcgatt acttcactca atgcggggtt
     4501 tcattacaat ggcgcgtccc ttgcattaag cactcttgag cctgttgtgc ctcaggaatc
     4561 caactttagt cagtacgaag gccctgttgt taaatcctgg gcagaagatc ctttttcaag
     4621 gggatcgtat gcgaattatg gtgtcgcact ctctggttca aagggaggac ttcaagccca
     4681 aaaaacaacc tatcgtggtg tgccagtgcg cagtcttttt gcgcctgttc atgatcgtct
     4741 ttttttcgca ggagaacaca ccacaatgtt ggatgaaatt ggtacaatgg aagctgctgt
     4801 tgaatcgggg aataacgtcg caaagcaaat caacgctctc ataggttagt tttttaattt
     4861 tcaaaatacc gttaattctt cgctttaaat tttttaaaaa tctatattgt gtgttaatgt
     4921 tcataaatat ttaacttatt ttcgttaatt tcaaagaagg cagcattgat tttatgtctt
     4981 ttgtgaccaa actctataag gataaacgct ttgcaaccct tttatgcctt ggttttgtaa
     5041 gcggccttcc ctttttatta accctttcta ctctcagttt ttggctcagc gaagtgggta
     5101 tttcaaaaac aaatataagt tttctcatgc ttgcagggat tccctatagt attaaattct
     5161 tatgggcccc ttttttagac caagtccgac tccctttcct tacatctttt ttaggacatc
     5221 ggcgtgcctg ggctattttt gcgcatttag ggcttatggc tgcacttttg tgcctcggct
     5281 ggacagaccc caaaaacaat ctctcactga cttttcttgc cgctcttgcc gtttctcttt
     5341 ttgccgcaac ccttgatacc gtcattgatg cttatcgtat tgagctttta ccagatagac
     5401 accgtgccat gggagcatcc gttgaggcca ttgggtttcg tattggta
//
LOCUS       DAIAHG010000074         5426 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_74_length_5426_cov_2.985418, whole genome shotgun sequence.
ACCESSION   DAIAHG010000074 DAIAHG010000000
VERSION     DAIAHG010000074.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74_length_5426_cov_2.985418"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1458 a         1138 c         1117 g         1713 t
ORIGIN      
        1 cttgggtgcg caaataaatt ccatcaatca catgacgatc atagcggctc agtttgagaa
       61 aaatggctgc aagagtcgta acggttccag acgttcccac catctgtact tgtttatttt
      121 ctatataggg tgcgatatta tttttttctg caaataaatt aagagcggtt gttgttttat
      181 caataatctg ctcgtaaata cgcggcgatg ttgaatgact ggcgtactca tcacttaacg
      241 tcacaacacc aaagggaagg gagatccaat cgatcacttt aaaatctttc acattatcgg
      301 aaagagagac ccacatgact tcagtgcttc cgccaccgat atcaaaagca atgccatagg
      361 gaatgttgtt ctgaaaaacg cctgagcatc ctataagagc aaggagagct tcctcattgg
      421 gggcaataat ctcaaggtca agccccaact ctttttttgc acgctccaca agaacatgtc
      481 cgttcgaggc acgacgacac gcttctgtgg tgacggctct gagtcttgta acattgtttt
      541 gctgtacttt atcgacacaa atcttaagcg ctttaaggct gcgctcgatc gcatcctctg
      601 aaagtcgatt atcttcttga agattttctc caagccttac aacttttgaa aaagagtcaa
      661 taatacgaat tttaccgtca aacttgcata tcaagaggcg acaggaattc gtgccaaggt
      721 caatggccgc aaaggtgtcc ttcatctcta ttgatttgcg tctgttgcgt ctaaaattca
      781 tttgctcaaa ataaccactc tacaaatcat ttatctcttt aaaagagtac cttacctatg
      841 ttaatacaca cctaaggggg cttttggcaa tcagggattt tgtttaaagc tctttatttg
      901 tgatggggga tagtttagcg gtagaactct cggctctgga ccgagcaact ctggttcgaa
      961 tccaggtccc ccagccatgc ttttaggcaa cttttaaaaa atctatggtt atttttatta
     1021 gtatctttgc ttttttgatt tctttcacgc tgtgtttggt ggggaagggg cgttttcttt
     1081 tggcatttcc aaacgaacgt tcagcacatc acaagccgac atcccgcttt ggtggaattg
     1141 ctatttttac aagtcttgcg actgcaaatg tgacctatac gtttttgaca ggggatatga
     1201 gttctgtcgt cacacttgtc atgacaatgg tgcttgccat tttggttatc ttaggtgctt
     1261 ttgatgaccg taagcacttg catcctctga ttcgactgat ggggcaaatg cttttcagcc
     1321 ttttagcgat gtatctcttg agttacaatt ttcccttttt ctcgactaat ttcataaata
     1381 gtgtagttgg cctttttctt atgcttgcct ttatcaacgc cgcgaatttt agtgatgggc
     1441 taaatggtct ttggtcggga acctttttgc tgtgggcggg atatgtggga tatttttgga
     1501 gcccaatgcc ttttttatgg atgatgatgg cggcgacttt tggctttttt gtgcttaact
     1561 tcccaaaagg taaagtcttt ttaggcgata gtggcagcac gcttttagga ggatttgcgc
     1621 ttctttacgg gctgtatctt tcccatcatt ttcgcttaga tttttatttt cttacacggc
     1681 tcaccattat ttttgcgccc tttattttta tttttgcaga tattgtgatg acacttgtgt
     1741 tgcgtgtggc acaagggcac agccccttta cgccgcacaa ggagcatttt tatcaaaaac
     1801 tgatccaccg cgtgggacta tcccatggaa cagtgacaac gctttatctt tttgggagta
     1861 ttttatcgag ttttttcatc ggttttttaa ctatgaagca cttctgggtg ataggatttg
     1921 ctttttatat tccccttcag cttttgtttt ttgggggcgt ctatcgctgg tgccatgttt
     1981 gtgggctttt gaaaagttcc taaattaaaa tgatagcgat cgagaggcat actcgcatcg
     2041 agcttcatag gtataccttt tgaggatcca tttccaatta aatccaaaag atcgaggcac
     2101 gtgagtggtt ttgaaaaata atacccttgt gcaaaacgac aggatgtttt ttggacaaca
     2161 cgaagctcct cctccgtttc aaccccttct gcaatggaga tcagatcgag atcctccgca
     2221 aggccaataa tcgcttgaaa aattttttct tttttagggt ccttatcgat attgcttata
     2281 aacgagcgat cgattttaat ataatcaaag ggataggcaa caagatagct aagagaagaa
     2341 taccctgttc caaagtcgtc aatggaaagt ttaaatccgg attttttaat aaaatcaaga
     2401 ttttgagcaa cattcgccga gttatccata atgacgcttt ctgtaacctc aaacttgata
     2461 ctttcttttt tgagacccga atctttaaag atgcgcacaa gggatttaag gtgatttaaa
     2521 tcttggacct gtttagctga aaggttaatc gcaatgttgg gcagccatgc ctcgccataa
     2581 gctcttttcc atgcgttgag ttgtttgcaa atggattcaa aaagatactc cccaatgtat
     2641 ttaataatgc ctgtttcctc ggcaatttga ataaatttga tcggagggat gagccccttt
     2701 tgaggatgat tccagcgtac aagtgcttca agctcctcta tttttccatt gcttaaatta
     2761 ataatgggct gaaaataggg aatgaattca ccccgctcag ccgcaatgcg aagttctgtt
     2821 tcgatttgca gtgtactata gaccaaatcg tattgatctt tctcaaagac ttcgacgcgc
     2881 ccttttccgt tgtgctttgc ttgatacatg gcaagatcag cattacgcat aatattttca
     2941 ggctcatcat acgcgtcaat aatcacaaca cccgcacttc ctgaaatata aatctgctga
     3001 ccataaataa aataaggttt actaatttcc tcgataatac gattagccgt tgctgtggct
     3061 tgctcctcgt tatcaatcgc cgcaaggaga atggtaaatt catcgcctcc aaggcggcat
     3121 attgtgtcgg atgaacgtac agtcagttca agtcgccgcg caacacttac aagaagttga
     3181 tcgcccgctt catgaccgag gctatcgttg acaattttaa aacggtcgag atctaaaaag
     3241 agaagggcag cctttttatg ctgagagcgc ttaaatattt tgagtgtatg cttcacacgg
     3301 tcggtgaaca aagaacgatt aggaagccct gtaagtttat catgaaaggc tgctttataa
     3361 agttgttcct catagcgttt tcgctgggaa atgtcggttt gggaacccgc aatacgcaag
     3421 ggttttcggt tatgatcccg taaagccaaa ccttttgata agacccaaag ataactccca
     3481 tcacaacact taatttgata ttcagcctca aagcgctctg taaaaccatc gaggtgtcta
     3541 aaaattgctg atctcaccga tttttgatgt tcaggcgtga tgcgataaat ccaatcatca
     3601 atggatgtaa agtgatcata ttttgaaaag ccaataattt ccttccaacg atctgaataa
     3661 aaacaaacat cattctcaat atcccaatcc caaagaccat cattggcgcc aagagaagca
     3721 agagcatagc gctcttcatt cgctttagcc tcgctcattt gctcccttaa gtcttcgttc
     3781 aatgttgtca actctgccga catcattgtg agcgattggt gggatagata ttcctcctcg
     3841 tcggctgttt ggtaaacatc actgatggat tttaaaagac catcgatatc aatttgtccg
     3901 tcgtctttgg taaaacgttt taactgcctt ttcaggattt tatgtaaagt tgtggtctca
     3961 accatgcatg ctcatccttt tatgaaaggt cttccgtaaa ggctgcaatc gtgactgttt
     4021 gattgtgtag ctcaggaatg cccgaatagg tatgcggaga aatttcacca taggaaaaga
     4081 atcctgcacg ataaaaattg gggtcattta aaatggtcga tacagcttca atttcatcaa
     4141 tggaacgcgg tcccatcata agttttcgcc cgatacagct aataataatc gcgacagaat
     4201 tacttttgag atgagcgtgt gggagatttt ttttaatagc ctctgcggat agttcggcac
     4261 catctgtaag attttttgct gtgccgctca taagctgagc ggaatatccc tctggaatat
     4321 cgccagcaaa agtcatgctt tgatcctctt cgttgacgcc caaaatagtg cgcactaaaa
     4381 aagccttggg gtcactgggt ggatggatac gcaatggaaa gagaagcgcg cttgagggaa
     4441 gttcattcgc aaacttgcca agataggatt tatagattgt tagagcaggt tgaccatcca
     4501 gttcatataa aatatttccg tgtgattttg tcacaatgcg ctctcgacca aaaggctccc
     4561 aacccccttc agagccccag gcaaaacgga tagcatctcc ataaaaacca atagccgcaa
     4621 tcgatttttc tgtaggctct gcgtcaatgc ctacaagagt ttgctgaaac tggcttccat
     4681 ctgcagcgag gccccccata atgggaatgt cttcaagcgt gcttttgagg ccattcacaa
     4741 gctcgcttcc attgacagaa agcccatctg acagcacaaa aacgccctta agtgttgcat
     4801 ctttaagttg gagggcaaga ttttgaccgg cttcaaaaaa atcactgtta ttgttaattt
     4861 ttataaaagc tttttttagc gttgtttttt caaattgaat tgcaagactc acggctgtgt
     4921 tctcatgcac atcatcttgt gcaatctcac cccctgtgct gcaccccata aggattgaat
     4981 tgggataagt ttttttgagt gcactataaa tgctgtggtt ttgaagcgtt tctctgtgac
     5041 taaaataaaa gacaagatcg gccgagtctt taagctcaga ggattcaatt gcatagtgtt
     5101 tttcaggggt taaatgattt ttctcaacct tcattccaaa tataccgaga tcacatgatt
     5161 ctagtaaaaa gtatgaggtg taaaagttaa tatagtctta ttttttgata aaaatgttgt
     5221 atatgctctt aaatccactg cagttttttt tgcagcgccg ttgagattgc ttctctctca
     5281 tcaaagggtt tgacgctttt ttcatcgctc agacgaccat caaaccactt tacacggcct
     5341 gtgtccataa tatgcttatg aaaatcagca aattttccgg gattgccctt aagcggcaaa
     5401 aggtaaacag gttttgtcgt cgcaca
//
LOCUS       DAIAHG010000075         5417 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_75_length_5417_cov_3.946255, whole genome shotgun sequence.
ACCESSION   DAIAHG010000075 DAIAHG010000000
VERSION     DAIAHG010000075.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75_length_5417_cov_3.946255"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1598 a         1154 c         1164 g         1501 t
ORIGIN      
        1 taaaatccta aaaattcacc tattatcacg cctaatgcgt taaaaaagct attttttgcc
       61 cgaaaaacac acaaaatagc gaaaaaaata ggattttcga aggaaaatct agtcgcacaa
      121 aaatagcccc atttttcacg aaaaaccctc gaaaattccc caaatggacc ctaaaaaggg
      181 ctattttcaa agaaaaaggc ggatatccaa tcatgctttt aaccgacaat aatgcgtcaa
      241 atcaggagat aaaagcttca atttactatt tttctcgcca aaaaagtcct cataagacca
      301 aaaaatgacg ttatatccag gaaaattcct gcgaaatgcc actaaaacac tcaaaaacca
      361 caaatattag ccaaaaaagc gcttgcaaga atataaaaac gacattttat cgttaaaaat
      421 cattgaagac gaattctttt attcttcctt ctcgttcaaa cggtcggcaa gggctgcttc
      481 taaaaacaca tcgatatccc catcaaggac acctgcagaa ttccccttct caacgcccgt
      541 gcgaagatct ttgaccattt gatacggctg cagaacatag gagcgaattt gatggcccca
      601 cccaatctct gttttgcttt gattgccggc cgcttcctca cgtttgcgaa gctcttgctc
      661 gtaaagacgc gactttagca tggccatcgc ctgtgcacgg ttgcggtgct gggagcgatc
      721 actttggcat tgcacaacaa tattggtggg caggtgtgtg atgcgaatgg cactttccgt
      781 tttgttcaca tgttgccctc ccgcaccaga ggcacgatag gtatcaacgc ggagatcttt
      841 gtcttcaata ataatatcaa tgctatcatc aatctctgga tacacccaca cagaggcaaa
      901 acttgtgtgg cgccgcgcat tcgcatcgaa aggagaaatg cggacaaggc gatgcacacc
      961 agattctgtt ttaagccagc cataggcctg atacccgatg atttttaagg tcatggattt
     1021 tatgcccgct tcctcacccg gattttcgtc aagaatttcg actttatagc cccgtgtgtc
     1081 tgcccaccgt gaatacatcc gcgccatcat ataagcccag tcttgcgctt ctgtgccccc
     1141 cgctcctgca ttaatttcaa caaaagccgc attcccatcc gcctcgcctg ataataatgt
     1201 ctcaaggcgc agtcgcgcgc atacttgatg aatgaggttc agtgcttctt ctccctcctc
     1261 aagggtggct ttgtccccca tttcctcgcc cagatcaata agcgtgagcg catcgtcaat
     1321 ctgacgttca atatctttga ttttttgaat actgcgtgtg agagcttctt tttctttcat
     1381 caaaagctgt gctgcttgag gatcatccca cagtgtgggg gattctgctt gtgtttcaag
     1441 gtcgcttaag cgtgagattg cattatccca gtcaaagatg cctccgaaga agggcggtgg
     1501 attctttgat atggttaagg acgttcatca tttctgcgcg catcgtatcg acactcttaa
     1561 aaattcattc gacactagaa ataacacaaa aaaacacgac aaaatacgaa aaactgtgta
     1621 gattgaggga taattgactt tagtgaagta gtaggccctc gcgatgaaat tccttttaac
     1681 ttttttatgt ctcagcttcc ttgtggcgtg tggaaaaaaa gattctgacc atcgtacgct
     1741 tgtgattgga acgtctgtag ataatccgcc ttttgaattc tatcaaaatg gtgaaatgac
     1801 aggatttgat atcgagcttg gaaaacttat tgctaaaaag ttagggcgtg acgtgaaatt
     1861 cgtagacatg tcttttgata cgcttatcgc agcacttcaa gcgaaaaaag tggatatcgc
     1921 catggccgcg ctggctccga cagaagagcg cgctaagtcc gttgattttt ctagacccta
     1981 taatgaatcg cattttgctc ttgtgactgt tggcctgacg gatattaagg attttaaaaa
     2041 ccttgaagga caaacagtgg gcgtgcagtt gggctcaacg tatgagcaat atgctaaaac
     2101 tattttagaa aaatatgttc acgcagaggt gcgtagcctt ggcaaagtaa cggaccttat
     2161 tcaagagctg cgcatcggac gcattgcggg gcttattacg gggattgcag aggcggataa
     2221 aattattgaa atgaacccaa gttttacagc ggttaaactc cccgaagata gtgcgggaga
     2281 ggctgtggcg ctgcccaaag gttcttcttt aacagaggcg attaatacca tccttaatca
     2341 cttcgaagcg gatggcacat taagtaatct gcggatgaaa tggaagatta gctaatgctt
     2401 gagtttagtg ttatgacgga tgctctcccc tttatccttg agggcgttgg tgttacatta
     2461 aaatttacag cgacctccct tttgtgcgga ctacctctgg ggctttttct tgcttttttg
     2521 aaaattagtc catgcaagca tttggggcgt attgcacaga tctacacctc tatttttaga
     2581 ggcacgcctc ttatcgtcca actcagcctt attttttttg gtctgcccgt tattacaggc
     2641 tataacatgt caccttttgt ggcgggtgtg attacctttt cactgaattc agcggcttat
     2701 acatcggaaa ttatacgctc tggccttcaa agcattgacc aaggccagtg ggaagccggg
     2761 gttgtgttgg ggctatcccg catgcaaatt ttgcgtcata ttatcctccc ccaagcgttt
     2821 cgcctttctc ttccgtctct tgtgaacgag tggattgatc ttcttaaaga atccgccctt
     2881 gtgtctatta ttggagaggc ggatcttttt cgccgtgcac agatcattgc cgcagaaaaa
     2941 tatctctact ttgaaccgta tctgactgtc gctgtttgct actatatcat ggttttaatt
     3001 ttgacactcc ttgcctctcg attggaaaag agactgcgct atgactaaaa aacttgtttt
     3061 gaataatata tccaaagttg tggggtcgga tctgacaatc cttaaagaca tttgttttga
     3121 ggtggttgct gggaatattc ttgtgctttt ggggccttct ggcagtggga aatcaaccct
     3181 tttgcgggcg attgcaggtc ttgagtcttt tacaggcaca atgacgtggc atgggaaaaa
     3241 gctgaatgcg ctggataaag gatccattgg tcttgttttt caaaattttc aacttttccc
     3301 tcacaaaacg gtccttgaaa atcttttact tgcaccaatg ctgaaagggg agaaaaaaga
     3361 tgctttgaaa gcaaaagcaa aaaaattcct caagcatttt ggtcttttgg agaaggaaga
     3421 aagttacccg caccagctct cagggggaca aaaacagcgc attgcaattg cgcgggccct
     3481 gatgatgtcc cccaaaattc tacttttgga tgagccgaca tccgccctcg atcctgaaat
     3541 ggtcggcgat gttgcagccc ttcttgcgga tattaaaacg tcttgcgatc ttttgattgt
     3601 cgctacgcat gagctcaggc ttgcagaaaa aattgcggat gagatccttt ttcttgatca
     3661 tggatatgtc gctgaattta ggcacgcccc tgactttttc aaagatccac actctcaacg
     3721 cgcgaaaaaa tttattgcca acatgcatta aacaaagcaa ataactggga attcgcggat
     3781 aaaaaaatat tttaaaatta ttcgattatt ttttttatcc cccctctcca tctcaatcct
     3841 tcgaaaagaa caagcattga gagagacaga atgagaaaat agaggggcat gataatgagt
     3901 gatccattgc gaatggtcgc catgataaag gtgagcaagg atgtaataag ataataataa
     3961 caaacaaaaa gagcggatgc ggtaccaagc atgggttgat aatcttctag agcgttggat
     4021 aaaatgcttg gtcttgtaag gttgatccca caatacatta agatcataca caaaagaata
     4081 atatgaatag atgctcgagg tgattctcca tgaataattt caaaggagct gcatcctaga
     4141 aaagaaaagg aagctaagaa gcaaagagaa agacctaacc aaataatcgt ctccattgcc
     4201 ataccatgct ttaaaaaata cgtacaaagt ttagccccta tcatcgcagc aatggataaa
     4261 agaatgaaac ttgccccata ggcagagggg ctaagcccaa gcatttcgat aagatagaat
     4321 gatccctcac cataataact aaacataata ccattcatcc ccccaataag cgctccatag
     4381 ataagaactt tagggtcttt gataagaata cgcataacct tcaaaagaga gtaacgagag
     4441 cgtgcgtgtt gtgggtgagt ctcataaaga aattttttga tttgatagag aataagtcct
     4501 cctaagatca ccaaaaaagc gaacacacct cgaaacccaa agaactctgc aagccatcct
     4561 cccacaagtg gtcctatagc gggagagatg gagagtgcag caccaattgt ggaaaaagca
     4621 atgctgcgtt tgtttccact aaaggcatct cttaaaatga cttgcccaag ggtagatcct
     4681 gcacttcctc caaaagcctg gacaaaacga ctgagtaaaa gcacttctat ggacgaagaa
     4741 agatagcatc ctatgcagcc taaaacataa ataaaaaatc cacataaaag gggtaattta
     4801 cgccccaaca tgtccgacat gggcccccac ataagagtcc ccaaagaaaa cgcaaaaaga
     4861 tagatggaga gagtatgctc gaccatggca tgagatgtcc caagggcatg agaaatttcg
     4921 ggaagagatg ggctgtacac tgtctccgca aactgtggta agcctacaat aaagacaaga
     4981 agccataacg ggggttttgt cataaaatgc ctgtagtgaa aaagtgcgtt tttttaatga
     5041 gatatttaga atacaccaaa ttgttatatt gcggtgaatt ttttgacgat tttgcacaag
     5101 agaggggcgt aaattatcct caaaatcact ctttttaaaa attttgacga ttttagtggc
     5161 atttcacaag gatttttgct ttcttggccg agatcttgtg gtttttgagt attttagtgg
     5221 catttcgcag gaattttccc ttcttaacag tcgtatggac atcgggtttt tcaggcaaaa
     5281 aagggctttt ttaacgcatc atgcacgaaa ataggcgaat ttttaggatt tttgaggcga
     5341 attttcgcaa aaataattgt gctggaggga gctgaattca agggttctgg tctccttaag
     5401 gggtggcgat acggaga
//
LOCUS       DAIAHG010000076         5393 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_76_length_5393_cov_2.807938, whole genome shotgun sequence.
ACCESSION   DAIAHG010000076 DAIAHG010000000
VERSION     DAIAHG010000076.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76_length_5393_cov_2.807938"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1634 a         1055 c         1126 g         1578 t
ORIGIN      
        1 ttctatttta tcgtcgtccg ttttcttttt cctaaaatca tccactgtcc atattttcgc
       61 atcggagtct attttttgaa taagcaatat cccttcctta attcattgat ctgcatgagg
      121 attttttggc gtgaaatgcc atggcacagg acaattttct cagccaaaat tttttagtac
      181 taactttttc aaatactcct caattccata ttttctatct atttcctctt taaatggatt
      241 gagtgaacag cgataatttt caatcgcttt ctctaaagaa atctcgagtt tgatctctcc
      301 tttgaaaagc agccatgaca gagcatcacg tagtgagagt ttatttttct ccatcatcgt
      361 tttacactgt gacaacattc caagcacata ggccgcattg ttgcacgcat ccgttgtgaa
      421 agactcagta aaatatcccc cttcgagata ctcgttaaac cgaccagcat cataagtgtc
      481 aagaccgata taaggatatt ggcttccttt gatataggtt ctattatcat caacaaggat
      541 catgttccca agatcttttt gaataacggt taaatctttt agatagttcc catgagtttc
      601 caaatattcc tccagtgaaa ttctattcac tattgtacac tgcgctataa atgtacggta
      661 tttttcctcc ggtgtgaggg tgtgtgaggc gtaaatttta aactgtcccc cccttgacaa
      721 aaaggtcata gactttttcg cattcatcgc tataacggtt aaaaaaatct tttaggaatt
      781 ttggaattac atcttcatta cgctttttag tgctggagga gaaaaaatac acattccatc
      841 cccaggacaa aatttcaaga aaaccctcgc caaaatagta gaaaaagaca tagacgcaat
      901 tcttctcctc aaaagaaaac ggaatgattt ttagaggaaa acctgtgccc tcaaggatat
      961 ttttcttgaa attttttctt ctagacggat acaaagaacc tcgtcaatat caaaaacaac
     1021 cgttggcttt tcaggattac acgtgtaaga aattttttta tttttaaaaa caaatgtatc
     1081 atcttccatt tttttgctaa tattttcttc tgcaatttca acaattttat ccgttgcaat
     1141 cgggcccgtg cagtgacaaa agaaaaagga gaaacggtgc tattttgtga aaaatcatat
     1201 taacttataa ttaaatttta tatagctaac ccagtagaca actcctctcc tctttttaag
     1261 tgaaaaattt atgacagttc ccacttttga tctcgaacgc gcctccaact tttctgcggt
     1321 tgtagggatt gacgaagcag gatgtggccc ttgggcagga cctgttgtgg tggctgcggt
     1381 gatgtttcct tttccgcatt ggggaatgat cgagaaaaat ttaaaaataa acgattccaa
     1441 aaagctctcg gcgcaaaagc gtgaggatct ttttgagaaa atttgcgaca ttgcttattt
     1501 tagtatcgca gaagccagca ttgaggaaat cgatactcat aatattgtgg gtgcggtgcg
     1561 ccttgcaacg tacagggcta tcgaggggct aagccaatct ccagagcttg cgcttattga
     1621 cgggattcga aatccacaga tttgtatccc cacacagatg gttgtcaggg gggacgctaa
     1681 aagttgttcg attgcggcgg catcgatcct tgcaaaagta acaagggacc gcatcatgga
     1741 aaaacttgcc aaggattttc ccttttatgg atgggaaaaa aatgcaggct atggtacaaa
     1801 acatcatcaa atagggctcg cctcccatgg cattacgatg catcatcggc ggtcctatgc
     1861 gcccattcga aagattattt cagattttta aattataaaa aaaggaaaaa aataatgatc
     1921 aaaaaaattt tactcagcgc atctgcaata gttttctgtg taaaaactgc ttatggaagt
     1981 aacgtattgt catggtcagg atatacaacc gcaacacggg tgggtggcca ttgcaccagc
     2041 cttaatatca ccctcaatcc caatgaaaat ctcaagacac aaacagagaa acttatcgat
     2101 gctcataatc ttaaaaatca ctttgatggc agtttaataa cgaactttca acttcttgac
     2161 ccaagatcgc aggagaatat ctggttttct ttgacgggag acatgaagaa atatacgtat
     2221 agaaatgaca tgctttggca tttaatgggg aactctgatc aaaaatataa tgatctttct
     2281 caatgcatga aaaaggaaaa aaacaaagaa aataaaatag tcattaacgt cgatctgctg
     2341 agccaaggcc ttaatacatg tacgtatgaa ataccagttc ctcagataaa aaaagaaaat
     2401 gacattactg agacggtagt ggaaaaagaa gaaaatcgaa tgattgttga tcctgattac
     2461 tataagggaa ataagcaaaa tgacattctt aaggatattt taaaagaaca cactattaag
     2521 acggtgaaga aaaaagaaga agtctctctt ttcaaaaggc tttatcgtta ttttttcaat
     2581 gatagcggtg aataattttg aataagtaag attaccttga tccttggcca taaatttgtt
     2641 taactttgac atatttttat aaaaagtgag ataaatcatg aacaatatgc cacggatcga
     2701 gggattatgc tctcgtcaag cccatgccga ttttccagaa ggaacctatg agcgcgaact
     2761 tggccaagaa ggtttctttg gtctttgcac ccatatgatt catgagtacg cgccaacatc
     2821 gtggacccac tgggatgggc ctcttaaacc acgtgcgttt gatctgacaa aactaacaac
     2881 caatgagtcg agtccttgga aggcagtaag tgtgctgcat aacgcaaaag cacaggtgcg
     2941 ttggtggaat ctttcaaagt cgatgacaga tcttgtgcgc aatgctgatg gtgacgagct
     3001 gctttttatt cataaaggac aagctgatct ttattgtgat tatggccatt tagaagttac
     3061 agagggggat tatgttcttc ttcctcgggg tacgatgtgg cgattggatg tgatccatcc
     3121 tttgcaaatt ctcatgacag aagccacaaa tggtgcgtat cgtctgcctg ataaaggcat
     3181 ggtagggcct catgcaattt ttgatccagc gatgttggat gtgccgagta tcaatgaaaa
     3241 atttaaagca caaaagcata cgaatcccac acacattatc attaagcgcc gcaatcttat
     3301 cacaacggtg acgtatcctt ataatcccct cgatgctctt gggtggcatg gcaacctcat
     3361 ggttgtccgc attaatgtga aagatataag accgcttatg agtcatcgtt atcaccttcc
     3421 accaagtgct catacaactt ttgtggccaa aggatttgtt gtgtgcacct ttgcgccgcg
     3481 tcccattgag tctgatccag gtgcgcttaa agtgcctttt tatcacaaca atgatgacta
     3541 tgatgaattt cttttttatc acaaaggtga gttttttagc cgcgataata ttcatcctgg
     3601 tatgacaaca tttcacccag cagggtttac gcatgggcca cacccgggag cttttagaaa
     3661 aggcacagat tttgcgcgaa aagaaacaga tgagtatgcg gttatgattg attttcgcga
     3721 tgccgcagat gtgggagagg ccatgaataa tgtcgagtgg aaagactatc ttcactcatg
     3781 gaaaggcatc acaaaaccat aaatgagtaa taaaaatagg aagcatgtgt ttttctatta
     3841 aaattttata tttttctgat acgctaaatc ttgagcattt taattgtgag agagaaaaac
     3901 tatgacatct atcgtaataa cagcggcaaa acgttcgccg attggcgcat ttcagggcag
     3961 tctatcgggg atgaaatctc ctgaaatagc tgcgcatgtg atgaaaaatc tttatccgaa
     4021 ggagcattct ttaaaagccg atgaggttat tttaggatgt gttctctctg ccggcattgg
     4081 tcaagccccc gcccgtcagg ctacgctttt atcagggatg gatgcatcga catcgtcaac
     4141 aacggtgaat aaagtgtgtg gatcggggat gaagtctttg atgattgcag cggatcaatt
     4201 gcgtcttggt catgccaaaa gcattattgc tggtgggatg gagtctatga caaatgcgcc
     4261 ttatcttttg ctacgcgcgc gctcgggcct tcgcatgggg cacggggaca ttattgatca
     4321 catgatgtgg gatggtttag aggatgccat gcattcaaaa aataatacct ctcagcgcaa
     4381 ggctatggga tgttttgccg atgaaacggc ctcaaaatat ggtttttccc gccaggatca
     4441 agaaaatttt gcgataaaaa cctttgaaaa ttatcaaaaa gcccatcaaa agggttgctt
     4501 taaacatgaa atcgttccct tttcctttgg tgaaacactc tttgaaacgg atgagccccc
     4561 ttcaaaagta aaaatagaaa agtttggcaa gcttcgccct gcctttcacc ctgatggaac
     4621 agtgaccgct gcaacatcat cgtcaatcgc agatggggcc gcgattacac atctcatgac
     4681 agaagcccac gccaaggatc agggggaaaa gcctctcgct tttatcaaag gctatacgag
     4741 tttttcctgt gcgcccgaat ggtttacaac agcgcccatt ggtgcgatcg aaaaactttt
     4801 aaagcaaaca ggctggagct taaaagacgt tgaccttttt gagattaacg aagcctttgc
     4861 tgttgtgccg atggcggcta tgcatgatct taaaatcgat ccatcaaaag tgaatattca
     4921 tggtggggca tgtacgcttg gccatcccat tggcgcaagt ggggcgcgta ttgtgacaac
     4981 gcttgtccat gcgctgcgca cgcacggtct taaaaaggga attgcagctg cgtgtatcgg
     5041 cggaggtgaa gcaacggcga tggcgattga aataggcgcg taaaaaaaaa gagtttttag
     5101 tgaaaaaaac attttacatt caaattaatt aaaattagaa tgattgaagg gtaacttttt
     5161 ttagaaggat ttttttatta tgattaaaat gattgcattt tttagttttg ttttcgccct
     5221 ttctcttcaa ggagctgttg cactacaagg caatacagat ggtgattcgg gcgatagcag
     5281 gctagagaac cccaaaaagc ttgttcctca aaaaaatacg caagaatctg agtaaattag
     5341 aatttttgaa aaagcttaaa cccattcccc gtgaatgggt tttttttatg ctt
//
LOCUS       DAIAHG010000077         5383 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_77_length_5383_cov_3.236336, whole genome shotgun sequence.
ACCESSION   DAIAHG010000077 DAIAHG010000000
VERSION     DAIAHG010000077.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77_length_5383_cov_3.236336"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1891 a         1055 c          955 g         1482 t
ORIGIN      
        1 cataagttat gaaaacgata gagtccctcc tgcaaaaaat tacaaaccaa gccatcaagg
       61 gtgatgcccg ttttatcgtt caacttattc ttgactggcc aaagattgtc ggaagtcacc
      121 ttagcacaat cacaaaacct gaaaagatta tttttgcacc gaaatgtttt gagaatggtc
      181 gtttgcacat tcaggtcaag cattcagcct atgcctttga actcagcatg cagcaagaaa
      241 gtcttgtttt aaaaatcaat ggcttttttg gaaaaaacct tattaataaa attcttttta
      301 agcatggggg ggctgttttt aaagataaaa cccccataga aacaacgatg atcacaccgc
      361 cacacgtaag tcatgatatc attaaggctg caagtcacat tcgtgaccat gacctgcagc
      421 aagctctttt atctttagcg atgcacctac gatgatcgga tttaactcgc tttagtccgt
      481 cgcacacagg gttctaaatc actttcacta aaatcctttt ctgtgcgatg gatttttctt
      541 tcttgatcgt tatcatcacc attaccgaag aaaaagcggc aaatacgctc aacaatcatg
      601 gaaaatgcac cgtttcccat gatattcgac gctgtaaaaa tagaatcttg aagaatataa
      661 attgttgtca taagcgtgat catttcttgg tctaacccaa ggtacttgtg taatacaggg
      721 agaatcacaa gaacaccccc gcctggtacg cccgcacagg aaaatttagc cactccaaac
      781 atcacagcaa aaagaaaata ttcttcaaat cccggaaaaa cgcctgtctt aaaccaaaga
      841 agcgcaagac ctgtcaacgg aatagccaga acgtcaccca gaagatgaat attcactgtc
      901 gttggaataa taagatccgc aaatcgtggg tccttaagat ttttttcagc agcctccagc
      961 gtcaccggaa gtgttgctgc acttgacatt gtactaaacc ctgtcacacc cgcgggaagc
     1021 atgtttttca ggctatcaaa atattttcca atcttaaatc cactcccaat aaaataaaga
     1081 atgttaagat aaataaaaat aagggatatc gccaaaacaa aaacttgaaa atagctgtga
     1141 ataattgttg aaagcgcgcc ttcatactgg agcttcagca caaaaccaat cacataaatc
     1201 ggaagaacag gaataaaagt tttttgtaaa aaccatgtca cagacatgtg caaaaattgc
     1261 gcatatttct ttgcaatatc aatttttaga aaacttgcca taagaccaag acctattccc
     1321 gcaaacatcg ccatatcagg cgcaataagc tgaggcaggt gtagttcaaa aagaggattc
     1381 accgtgacgt tttgcgccaa gtctgctgtt gtcttaaacg gtgatagaag cggcaaaaat
     1441 gtatgcgcaa cccccccaga gacaagaacg ccaatgacgt tggataaaaa gaccatgact
     1501 aaaatagtga taagcaaaag cggtgctttt ttatcaagtc ccaaaagagc atcaaaaata
     1561 taggcaaaaa tcaccacggg aagtgctgtg atcaaaatat ctttaatgac acaacttgcc
     1621 gtaaaaagaa cttgaatggt tgcataattg aggaaatgcc caaaactaag ggcgagaaca
     1681 agacacagaa caagctgcac gggcaagtgt ttgaggtgca ctaaatattt ttgtaacatt
     1741 ggaagtctcc acatttttaa actgaaatca caccaccttt gtctgggaga tgggccttgg
     1801 gatttgtttt gtcaagataa aaagatgctc acccacaagg ggtcgatgtg gaagacgtcg
     1861 ttgtcgaagc aacagcaata agggtgtaaa aatcgcatac cataccccct attatggcat
     1921 atctttgagc caagttaaag agtttaatga ttatttttta actgtacgca gagaggatca
     1981 agataaattc cctcatccaa acctcgctcg aggagtcctt cttgcgcgat ttttataatt
     2041 tcctgtgcaa taactttttc ctccgttgaa agtcctccct ttggaatccg tatatactgc
     2101 tcttggacct tttcaaatgt ccatcctgcg ataagattaa gtgtatgagt gagtgcttta
     2161 tcactataga gaagccctac aaaaagagca gccgacgcaa aggcaaaagg gcttgcatca
     2221 atgccacgaa tctcgacaaa ttgctttaaa cgcacctctg gaaaagcaac cgtaagttga
     2281 tcattaaaat catcaagagt tgggtattct ccttcaaagc cagcaagctt tccctccata
     2341 aagtcacgaa aagatgcccc cgccacattg atatagcctc cattgcggta cacaaagtac
     2401 atgggcacat cgagagcata atctaaataa tcttcaaatc tcatcgagga aaaagcaaaa
     2461 ggcagaagac cgcaacgatc aggatccgta tcctgccaca caaaattacg aaaacttttg
     2521 tactcagatg gcttgccctc atacaattca gaatgtgcaa aaagccccga cgtaagagga
     2581 ttaagggcat ttgtgatcaa catcatctgt gccatatttt cctcagatgt atagtccaag
     2641 tttgcctgaa gcgtacatgt ccgccgcatc atatctaacc catgattccc tttaagcggc
     2701 atatagttgc gcataatttc atagcgcccc ttaggcatcc aagacagatc ttcttggcgc
     2761 catagcggtt caaaaccaac ctcacgctta aaaaaattat atttttctaa aaaaacatct
     2821 aactgcgtat catactcctg aatttcatga aaggcagcgt gtaaatccgc gtggggtttc
     2881 gccgcaatct caaactgtcc tcccggctca agcgaaagag acgttccctc ttttttgcac
     2941 ccgatcaaaa actctttttc aaaaacaggc gcataaccac tttcgacaaa atcctcaaga
     3001 atctgcctga tttgcgcata cacaaggcgg cgtcctgtac ttttttcgta gagaaaacat
     3061 tctttttcaa taccaatgcc gcgtctatct tttgatttta ttccacgttg aaaaacttga
     3121 agaaagtcgg tgtgtgtgag gggcataaaa aaatccttaa aatctactta cttttgcaga
     3181 aagtgcgctc ttcttcaagg ggcctatccg tattttttga aaatgcagaa ttatcattga
     3241 cagcctcaaa gctttatgtg aataaaaaga tgttgatcta cattttatta aaattggatt
     3301 taaaatatga aaaagctttt tcttcccctt atggtttctt ttttatcgat tgcaaccgtc
     3361 tcaggcgcca tttgctcaaa taataatgat gaaaaaaaac aaaataagac aaatgaaacc
     3421 ccaaaatatg aaacacaaaa aagcatctat catcctcaaa ctatttatat aaaaaacgat
     3481 ctaaaagacg ttacgaatta taaaattttt ggcgaattca atatgaatat tgatttaggc
     3541 gaaaacacgc ttaacaaaaa aacagcattg gagataaaaa aatataatct tttccccaaa
     3601 gatctttccg ttggaatcaa ttataatgag tccgtcaaaa catttaagga aagcgacatt
     3661 tacaaaaaaa cccttgagac gattaagaaa agctttgaaa cagcgacggt cgatgtctcc
     3721 ctcattggca ttatctctga atattctgag gaacaaaaga gtgccaataa caacaaaaag
     3781 aaaaaaaata aaaacaataa tccacctaaa ataaagaatt catcgactta ttctcttgaa
     3841 aattatgagg gtgagaagat tttggaaatt attttaaata agcaagacaa caaagaagtc
     3901 ttaacacgca agccagaaga aaaatctgaa tccatctcca cagaagataa aattagcaat
     3961 ctggaaaaag acctcaataa acttcttggc atcaactaaa atttaatttg tttgttttag
     4021 cccgcaagaa ctcccttggc tttacgcttt gggagttttt ttgcataatt gtttttagaa
     4081 aacatctcac cacaggagaa aatcgatgct acttaatcat aaattttttg ccaacattgc
     4141 gatgattatg ctgctcgccg caaaaataac aacgtctgct cttgctagtg ataaaaaaat
     4201 actgacggaa aaagattcga agcattataa cgatgcgagg tattatatta atttttcagt
     4261 gaacattaca ccaagcaaag agaatatttt tgcgctcaaa gaggggggtg aaaacgctct
     4321 tcagaaggtc tttaaagaaa aagagtgtgg tcttatcctt tatatagata atgaccttaa
     4381 agatgatgcg gtcgataaaa cgtgggattt aggactagag atccttgaaa aaagacttaa
     4441 tgattttggc tataataatc tcctaaatct acaattttta tcctgtggta tgacagatga
     4501 aggagcctta tctgttgcaa atattattaa aaataatcaa ggtcttcaga tttttgatct
     4561 gagcacaaca ttttttttgg ggaataaagg tacaaaaatt ctaacaaatg aacttaaatt
     4621 ttgccaaaaa cttcatgacc tcagaatttc atctcttatc tattttgatg agattggaaa
     4681 agaggatatc tccgatgaca ttattgagct tataaaaaat acgtctcttc ataacattga
     4741 cattccatct cttccagaaa taggagcaaa aaagatcatt aattttataa tacaaacaaa
     4801 aactgatatt gaatacttag gatttgctgt ttcagaaatg actaaagaag aatatgagac
     4861 actcgatatc acgttggatg ttaataaaaa acgcaacaaa aaagagtaaa aacatattaa
     4921 attctttgtt tttttatttg taggatatgt ctcctattga tgagatgagt aaattatgaa
     4981 aacagactat aaattctttg cagcaacagc cctcattatg ctcctgggtg gccaaatact
     5041 tgccacagaa cagattaatt cctggaaaat ggatggaggg ggagaccctt ttaggggggg
     5101 cacgccatgt ccttaatggg cagagatcat tcagttcctt taaaagaact agatgattta
     5161 ttggaaaaac ttattcaaga gaaagatgag aataataaag ataaaatacg ccatgaaatc
     5221 gttgattatt tttttaaaaa atatgggttc acatataacg aaaatgataa taaagaatct
     5281 ttgacaagtg acaacgttga gaaaaaagaa aaatctttca aaagcaatat cgatatggag
     5341 taaaatttat accccctata aattttagga gagggagtcc ttt
//
LOCUS       DAIAHG010000078         5346 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_78_length_5346_cov_3.646612, whole genome shotgun sequence.
ACCESSION   DAIAHG010000078 DAIAHG010000000
VERSION     DAIAHG010000078.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78_length_5346_cov_3.646612"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1568 a         1001 c         1230 g         1547 t
ORIGIN      
        1 gcccatatca aataaatcat tttgaatgtg ggacaataca cggtcgatgt cctcatgatc
       61 tttggtataa aggcggcaca ggccgaggct ggcattcact tcatccacgg tgccaatggc
      121 ctcaatgcgc acactcgatt ttgaaacacg ggtggcattg ccaagggacg ttgtgccttt
      181 gtcgcccgtt ttagtgtaaa tacgtgaaag aagaaccatg tgtgctgcct ttttcctaag
      241 attttagcca tagcatgata aaaaaaagaa ggagggcgag tccttgaaga aaaatgcgcg
      301 ctctcatgag agcatttgcg tgttttttgg cataagcacc gcctttgata aagctgaaaa
      361 gaccgataaa aagcgaggca aggacagcga taagggaggc aataaaaagc acagagatga
      421 tggtcataaa cgcaacagcg ctaaaggaga cgtgcgctat tcatgcgctt attttaaaaa
      481 gaggtcaagg attcttgtcg tagaattcgt gcactagaat tttcagctta gaaaagttcg
      541 gtggctttct aaggattaaa tatcttttgc gatgatcaac aaaaacctaa aaaagtatca
      601 cgcgttgctt taaaataaat ccatgttttt tgaacattaa aaatagaaaa tacttaatgt
      661 tgttcgacga aaaaattgct gcttttataa aggattctgt attgccgcat catacgtgtg
      721 tggtagccgt atcggggggg gcggacagta tggcgcttct ttgtctcatg caagggctgc
      781 aggaaaaata tcattttttt aaacaaatta tggctgcaac ggtggatcat ggacttcgcg
      841 ttgaatccca ccttgaagcg gctcaggtca aaatgtggtg tgcctctttg gggattaatc
      901 atgtgacgtt ggtgtgggag ggggataaac ccaagtctgg tgttcaggca aaagcacggc
      961 aagcacgcta tgacctttta acacaccatt gcaaggaaaa aggtgcaaaa tatttaatga
     1021 cagcgcacca ttttgatgat caatgcgaga ctttttttct ccgcttgctc aaggggagcg
     1081 gcgttcaggg attgcggggc atgcgggcta ttcaaaataa aaagaatatc acgcttctaa
     1141 ggcctttttt atcgataacg aaaaaagagt gtttggatta tctggaaaaa atcagtcaag
     1201 cctatctgca tgatccctcc aatgataatt tagattttga gcgtgtaaaa atgcgccaga
     1261 tgctgagcga tttatcacaa aaagggcttg atacacaggc cattggttct acgatacatc
     1321 actgtgagga agtttcagat tttattgaaa ggggcgctgg accgttcatt gaaaaaatcc
     1381 ttaaagagaa aaaacttgtt atggatgatt tctctcgctt ggatgtgttt cttcaaaaac
     1441 atattctgcg tcaggtggcg tgggcgcttt ctcaaaaaga ttatccgcct cgatttaggg
     1501 atgtggaggc cattttgcat gctatcgagc accaggaaaa ttttactttt gcaggctata
     1561 tctggcgctt taaaggaggg atctatactt taatcaagga aaagcgtcgt gaaaaagagt
     1621 gataaaaaat tgttttttac gtggcattaa ctttcaacac gtagtgtgta agagtggtta
     1681 aatttttata aggtatagac agtgtttatt ttcttttttc tcgtatttct gatgaatttt
     1741 tcggcctccg tagcgatgga ggaaaaaaaa gagattacgc ataaaaaaat tacgaaggga
     1801 ctcgatccta acgatcggcc taaatggctt cagattgaga tagacgcgac atttaaaaat
     1861 ctttccgagc aaaaaaaggg gggatggaat tttaatggga acagttccta tagctttttt
     1921 ggatattcga atggtcttgt ggcctcggat tttctttacg ctatgataaa agcttaccct
     1981 aaaaaaaagg attttttagt tctggatatt ggtgctggag agggaaattt tggtcatgct
     2041 cttttcaaaa aaattaataa tgataaaaca cttccagatg atctaagtgt cataattgtc
     2101 agtattagcg gggaagatta tggtgatact gtggaggagg gtgaccgtgc gactcaccgc
     2161 atgattggag ggtttaaaat tgaaaatctc acagaaagtt ttaaaaaaat aggcatagat
     2221 cctgttggga catttgatct tattgtttct gattattgtt ttatgcatct gcatgatcct
     2281 gtgggaacct ttaaagaggc gtatgatctt ttggataaaa atggatttat gatgatgcaa
     2341 ggatttcctg tgccaggagt agggttaaaa gatcttcttc tcgaaacaaa agccccgttt
     2401 cttatccagg gagacgataa taattggcgt catcagccct cttttttgct gagaaaaact
     2461 gatggaaaac ttttaaatct accgctggcg tacacagaag attatgaaat aatagacatt
     2521 gcgaacacgg ccccccaccc tgtgcctgtt tataaaaatc tatcttctga cgtgtatgaa
     2581 acggttcacc ttaagcgcga taaagtgtac ggggataaaa atctttatct gtggttgatc
     2641 gagcattatc cctattggca aacgattaag acagagtacg cgccgttgct taaagactct
     2701 aaggatgagc cgatcacgac acttcctccg cctctgtttt gggcgtgcta tgaagacaat
     2761 gcaaaggaat tggataagct tttaagcgag ggggcggatg tgaatgcaaa agatgctttg
     2821 ggtcaaccgc ttttacataa agcgatccct aagccagaaa tgcttaaggt tatagaggcc
     2881 tataatccga attgggatat tcgtaatgcc tctggaaaaa cggttcttga ggcggctatc
     2941 gagcgtttct cttgggggat gagccctgag agtattgaga aatatctcaa tgagatccgt
     3001 gagcttattc aaaaaggatg tggtgtgaat gtgcaaaata gtgagggcaa cacgcccttg
     3061 catctggcac tgacgctcaa atacaaaaaa cctgagtata aaaataaaat catcaaactt
     3121 ctcttagcag aaggtgcgaa tccgaaaatt aaaaataaca aagggcaaac agcctctgat
     3181 cttgtgggga aggacaatga agaacttgca gatcttattt taaagcatga gtgatttttt
     3241 ttataaggat tatgagtgag gtgcctctgt cgggggaggc acctgcgtga ttagtcgtta
     3301 acacccaaat gacattttaa gaggttttca aatacttttg cctgaccatc aaatgctatt
     3361 acggaatatt tgtcgtaaag taggactaag gtctcagatt tattctcttg gtttctgagg
     3421 tctaaagaag tacctgcata gggcgttcca acaatattct taaaaattct gccatcttca
     3481 aaggatacta ttataccaaa aggcttatta taatcggaag actcgttaac ggatacttcc
     3541 ttgataacgc cttcgaattt ataatatgga tcgaccgtga atgcatgagc tacgggctgg
     3601 aagtttacaa aactcttcct cttcttctct tctttgtttt cttttaatac atcatcctca
     3661 aaggtgcttg caaaagctgt ggtcacgggg ctactgagga gccctaagga tagaaatgtg
     3721 atgaggtaaa gttttttcat aaaaccaccc tttctgttaa aagataaatc caacagtttt
     3781 atgagagagc aggaaaagtt aacaaaaggt taatattttt ccaaaaattt ttattttttt
     3841 cttttgtcct tttgtttgtc ctcattacgc cttccatcat agggattttt ttgaccacgt
     3901 acccaaaagc gaatcggcac gccctcgata ttaaaactga tgcgcagatt gttggtgaga
     3961 tagcgtgtgt aggattcagg aagtttttct gcctgagagc aaaaaagaat aaaggtcggc
     4021 gggcgagact tggcctgagt gatatatttt gggcgaatgc ggcgtccgtt cacagcggga
     4081 gctggatgat gttcgacaac atcgcggagc cactcattca actttgccgt ggaaatgcgc
     4141 ttgttccaca tcatatccat tctgaggacg gcatccataa gtttgccaag gccctgattt
     4201 ttcgtagcac ttatccctat gaaaggaatt tccttagctt gagctaactg atgcgtgagt
     4261 ttgtgttgaa tattttttaa aaacgcaggc tgttctttta atggaataag atcccactta
     4321 ttgagcgcaa tcaccatggc gcgcccttcg ttataaattt catacgcaag ggataaatct
     4381 tgtttttcaa gggcctgggt gcagtcaatc acaaggataa cacactctgc atattgcaag
     4441 gctttgcgtg catcttgcac ggacaaatgc tcgaccgagt catcaatgcg ggccatacgc
     4501 cgaatgcctg ctgtgtcaat cagctgtatc gtacgatctt tataggtcca ctcaatacga
     4561 attgcatctc gcgttaggcc tggcatatcg cctgtaagtt gtcgttcctc cctaaggagc
     4621 gcattaataa gtgtggattt gccgacattc gggcgtccca taatcgccag atgaaggggg
     4681 ggcgctgctt tttcttcatt ttcacttgtt tttgggataa gggggcttag aaagtcataa
     4741 agatcatcca tcccaaggcc atgctctgca gaaatactaa taatattttc aaagccaagg
     4801 cgtgcagcgt caattacgcc ctcaatatga tcatgactct ccactttatt ggcaagcaca
     4861 caaagaggtt tgtgataagc cctcataagg cgcccaaggt caatatcata agggttacaa
     4921 ggtgtgcggc catcaatcat aaaaagaaga acatccgcct cttcaatcgc tttttttgtt
     4981 tgtgcataca tcgattgtgt cagggccgaa tcgtctggat ccgcaaggcc ggccgtatca
     5041 ataatgtgaa aggtcaggtc atagagcttt gcatcgccat agcgccgatc gcgtgtgaca
     5101 ccggggcggt catgaacaag agcaagcttg cgccctgtga gacgattaaa caaggtcgat
     5161 ttgcccacat tcgggcggcc aataagcgca attttcatca aagaatcaaa agatattttt
     5221 ttactatata taacgacgat tggcgcataa tgcgacctgt ttctggtcga gggaggatga
     5281 tcaataaaaa aaataatatt ttgagcattt tatttactgg ttgttaactt cttatgttat
     5341 agaatg
//
LOCUS       DAIAHG010000079         5264 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_79_length_5264_cov_3.940042, whole genome shotgun sequence.
ACCESSION   DAIAHG010000079 DAIAHG010000000
VERSION     DAIAHG010000079.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79_length_5264_cov_3.940042"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1347 a         1057 c         1227 g         1633 t
ORIGIN      
        1 caaaaattgc gcaaatacga ggtaattatc atgaaaaaag ttctataaag gcgaaatatc
       61 gcctcaagca ggcttttttc gatccgacaa tgcctttttt aatgggaaat ccctcaaccg
      121 cgcccaacgc tttttctttt gcgaacacag tggatcaccc cctttatttt tttcctttgc
      181 cagacgcttc aacagtctag acattttatg gggatgtgat ttatggtgaa gcatccgatg
      241 ttcccgtagc tcagccggat agagcgcagg attcctaatc ctgaggccgt gggttcgaat
      301 cccgccggga acgccacttt atcactgcga ggttttatga gtctaggtac aacgccacaa
      361 ctaaaagctg ttccgctgcc tgaggggttt acttatgtga atacgctcca tcctgattgt
      421 cgtatatcag accgctatca aagcactgat aattttttag gtcgcccttt tcctcactat
      481 tataatggtg cgattatttt atcaacgcat gcagcagagg cgcttattct tgtccaagaa
      541 gctgtggcga aagatggctt tgaccttgtt ttttatgatg ggtatcggcc tcaaacaacc
      601 gtcgatgctt ttatcgcgtg gggaagcgat atcgcggatc aaaaaatgaa ggctgaatat
      661 tatcctcgcg ttgataaagc gcataccttt gatcttggat atattgcaaa gcgctctggg
      721 catacacggg gtagcacggt tgaccttaca ctcattgaaa aagggaaaaa acttcagcaa
      781 acacaaaagg tgaggcgcca acttacagat ggctttgagt tcacatacct cgacgataat
      841 acggtggata tgcatacaca tgttgattta tttgacaatg cgtcgcacca tgatacagat
      901 cttgtgccga aagaagcgac agctaggcgg aattatttaa ggagcgctat ggttgcggct
      961 ggttttaaac cttacagcaa agaatggtgg cactatacgc ttgcggatga gccttttccg
     1021 gaaacctatt ttgattttcc cgtagcttaa atttttaaaa ataagaattg agaaaccgaa
     1081 tgacgacctt ttccattgcg cataaaaatt ataaaaaacc tcttttaaaa aacattcaag
     1141 gacctgttct tgtgacagga acgggggggt tcattggttt tcacgcggca aaagcgttat
     1201 tggatttggg tgtggatgtc gtcggtgtag ataatttaaa tccttattat gatgttaaga
     1261 ttaagcatga tcgcacggcg cttttgaagc actataaaaa ttttaaattc tatgaacatg
     1321 acttaagtga taaggaagct atcgaagaac tttttaatcg ctatcacttt acccatgtga
     1381 ttcatcttgc ggcccaagcg ggggtgcgct attccctgac gcatcctttt tcttatctta
     1441 aaagtaatat tgatgcattt cttgttatgc ttgagtgtgc ccgccacgcg aagaattttc
     1501 agcatatggt ctatgcaagc tcttcctctg tttatgggat gaatacaaaa atgcctttta
     1561 gtgaggatga tcctgtggag cttcctatgt ccctttatgc agtgacaaag cgttcgaatg
     1621 agcttatggc acagagttac gctcatcttt ataagatgcc actgacggga ctgcgttttt
     1681 ttacagtgta tggtccttgg ggccgtcccg acatggcagc gtttaagttt gttaaagcga
     1741 ttttggaaga cagccccatc gatgtgtata atcacggaaa catggcgcgg gattatacct
     1801 ttgtggggga tacggtacgg ggtattttag gggctcttga tacgcaagcg gatgtgactc
     1861 agcaaaaaca tcctgtgttt aacttaggga ataataaact tgaaaccttg gcgtatttta
     1921 tcgaggtgct tgaaaaacat atcggtaaaa aagctcttaa gaatatgatg gatattcagc
     1981 ccggggatgt gcctcaaacg tctgcggata tcacaaaggc acaggtggct tttgactact
     2041 caccttccac agatattgac gaggggctga aactctttat cgattggtac aaagattatt
     2101 acggcaaaaa atagcctgga gatgcttgag aaaagatgat cactctttat agctattttc
     2161 gctcttcatc ctcttatcgt gtgcgcattg cactgcatct taagggcatt gagcatcaga
     2221 ttattccgat tgatctacgt aaggatgagc ataaaacgcc cgcttttcaa gcacttaatc
     2281 cgcaacaaaa tctcccagta cttgtagatg gcgatctgac actgtcacag tctcttgtca
     2341 ttattaacta ccttgatcaa aagtatcctt caccctctct tctgcctgag gataaaaaac
     2401 ttttttggaa ggggcgtgag cttgctcttt ttattgcttg cgagatgcat ccccttaata
     2461 acttgcgtgt tctaaaatat ctcacagggc ccttgcaact cacagaagat caaaaaaaaa
     2521 catggtattt tcattggctc aaagaagggt ttgatgtgct ggaaaaagaa cttgcatgct
     2581 acgatgcgcc ctatgctttg tgcgctcaac ccacccttgt ggatctttgc ttgatcccgc
     2641 aaatgtataa tgcacggcga tttggatttg atctaacgct ctatccccgc ttgtgtgcga
     2701 ttgaagcatc atgtttagct ttggatgctt ttcaaaaagc tgcgcctgaa gctcaaatag
     2761 attgtgatat ttagtcgttt ttttaaaaga caatcgatta atctgaaaaa aatatggtga
     2821 tcgctgtcaa attattctag ttatagacgt aataggcgct ttttcttgaa acacacacaa
     2881 tcaatgtatc ttattttagg tcttgcgcgc tcgggtcttg cggctgcttc ttttttaaaa
     2941 gggaggggga ttgattttgc tgtgtatgac gatagcccgg agaagtgtgc agcagcagcc
     3001 tttgaaaaag ggtacaaggt ttttaccgaa agcgactggc cctctattca aacagttgta
     3061 gtaagccccg gtatcccttt ttcttatcca gaaccccata agatcattga aaaagcacgt
     3121 cgccagggat gtgagattgt gacggattgc acgctgcttc agcgctttgc accctctaaa
     3181 aaatatattg gtgtgacagg tacaaatggt aaatcaacca caacggcact catcacccat
     3241 gttctgtgct gtacaggaat tgatgcagtt atgggcggaa atattggcgt tccagcgctt
     3301 gaacttggtc ttgatcatga tgtttatgtt ctggagctgt catcctttca gttagagcgc
     3361 tctccttttt ttcatcttga tgtcgctgtt tttttaaacc taagtgaaga tcatttggag
     3421 cagcatggtg cgatggatca ttatatcgcg gctaaaaaac gcattttcca aaaggcaaag
     3481 caaaaaattc tgggtataga tgattcttat acgcccacac ttcacaaaga attttcagag
     3541 gcaattactg tttcatattc atctccaaag gcagagtttt ttttgagtca tctctatctt
     3601 aagggggggc ataatctgca aaatatgctt gtggcgtatg aggcactcag agcatttggt
     3661 ctggaacatg atattgttgt caaaggaatc gaaagtttta aggggctcaa acaccgtcag
     3721 caatatgtag gtacattcga aaatgtagcg ttcattaatg atagtaaagc aacaagcgca
     3781 gaggcaaccg cacaagcact caagaccttt gacgggatct attggcttct tgggggtaag
     3841 gataaatccg atgggattga aagcctatca ccgcattttt ctaagattaa aaaagcctat
     3901 ctttttggcg cggctaagga ccgttttgca caaaccctta atcagtctca agtcccttat
     3961 gctctttttg aacggctcga agaagcgctt gagggggcgt atcaggatgc gactctgtca
     4021 aaaagtagtg cgactatttt gctctcgcca gcctgtgcaa gttttgatca gtataaagat
     4081 tttgaagagc gtggggagca ttttattcaa catgttgggc atattctcaa caaaaacaag
     4141 gaataatcaa tggcgattga aacattttca agacgggata caagcctttt aggtcgctgg
     4201 tggtggacca ttgaccgttg gaacctctcg gcgatttttt tgctggtgtt gatcggagcg
     4261 cttttaagtt ttgcggcgag tccttctgtg gcagatcgct tgaaccttgg atccttttac
     4321 tttgtgaaac gtcattttct tatggcaccc cttgcgttag gggcaatttt ctttgtttcg
     4381 atgatgagtc ttcaaaacat caggcgactg ggatttcttc tatttatcat tgctagcgtt
     4441 cttttgattt taactctttt tatcggcatg gaagtgaaag gtgcgcgcag gtgggttaac
     4501 tttgcggggt tttctattca agcatcagag tttatgaagc ctgcgtttgc tgttgtgatc
     4561 gcctgggttt tatcaaaagc gcataaaaat ccgagatttc caggactctc aacagctttt
     4621 gggcttttat gttttgtgct gagtttgctt ctccttcagc cagatttagg catgtccgtt
     4681 gtgattactt taacctggat ttgtcaggtg tttgtggcag ggctccccat tatttggatg
     4741 gtgattcttg gcgtgatggc gctttttggg ttgctaggag cctacatttt tcttccccat
     4801 gtggcacgtc gtattgatca atttttaaac cctgtgtcgg gtgatccaac ccatgatctt
     4861 taccaggttg aacagtcctt gggagccttt ggcaatggcg ggttttttgg catggggcct
     4921 ggtgaaggca ttgtcaaacg ccatgttcca gacgcccacg cggattttgt ttttgctgtg
     4981 gcaggtgagg agtttggtct gattctttgc cttgtgattg tgggtgtttt tgcttttatt
     5041 gtgatacggg cgctgacgaa atcaatcctt gggacaagtc ttttttctat gcttgcggtt
     5101 gtggggcttg tggcgcagtt tgggcttcaa gcattaatta atatggcgtc atcactgcat
     5161 ctgatcccaa caaaggggat gacgatgcct tttatcagct acggagggtc ttctatgatt
     5221 gcgattggga ttggtcttgg gattgtgctt tcgatgacgc gcag
//
LOCUS       DAIAHG010000080         5263 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_80_length_5263_cov_2.680872, whole genome shotgun sequence.
ACCESSION   DAIAHG010000080 DAIAHG010000000
VERSION     DAIAHG010000080.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80_length_5263_cov_2.680872"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1721 a         1137 c         1027 g         1378 t
ORIGIN      
        1 aaccaaatct aattacacag cgaggctttt tctgttgttt ttggacacag caatcaaaag
       61 cacaagagcg caagcgccaa cacagctgct gaccaagaaa gccatcccaa cactattaaa
      121 atgctgtgca atgttccctg cgacggttcc tgcgatgata agcgagattg cgctaatgag
      181 gtaaaagaaa ccaaaggccg tgccgcgcaa atcttcgggc acagatttat cgacaagcgt
      241 caaaaaaacg ctctggctaa tccccatttg aatcccccaa atgcccacac cactaaacat
      301 gacccagaca ttgggcgcca tggccaggac aatatccgca agaataaggg ccgcaaaacc
      361 tacctgtaac agacgatatg gatccatacg gtcagccata cgcccaatgg gataggaaca
      421 caaagaatat gtcaaattat agacaaggag gataaagggg atataggtat cggaaaggtg
      481 aaacgtttca tgggcatgaa gacacataaa ggcttctgtg ggacgtgaaa gcataaaaat
      541 gctgactact aacattaaaa accaatagtt tttgccaagt cgtttaagat cagtgagacg
      601 aataggatgg cgcttaggat gtgcaatatt ttccccgaca tcaccttctg gaagagggac
      661 aggctttgga tcttttaccg caacaatcag gataatcagc gcaataaacg ctggaatcgt
      721 agcgatccaa aacacatgat gaaaattatt cattgtccac atcatggcca gaacgccaag
      781 aatgccgcct gcaaaacttc ctgctgtacc aagactttga cgcaatccaa agcaggctcc
      841 tttgatatct ttaggggcta cgtcccccac aagagcgtcg cggggcgtcg actgaatacc
      901 attaccgaac cggtccaaga aacgtgccat aaacaccgca ataaaacttt cggatatggc
      961 aagaataggc cttgtcaatg tgactaaaat aaaccccaca accatcacag atttacgttt
     1021 gcgcaaataa tcactgacga cacctgagat aagttttgtc gcatgggcac tggcttcaac
     1081 agctccttct aaaaaaccaa tccaacctgt ggcaacgcct aaaatcgatt tgagataaac
     1141 agcggataaa ctaaaaataa tcaccgagga aatattaatc aaaaactgga cgaggccaat
     1201 cgcaatgaca gtgggtggaa tagatgagcg ttcttttttc atcgaatgaa acgttccgtt
     1261 aaggaaatat taataaggaa accctatcat caacaatata gggatattta tcaagaaaat
     1321 tctgagaaat cacagattta caatgaaagg aagaacagtc atgtcacatt ttggaaaagt
     1381 tggtatcgtt acaggatcca caagcggtat cggtcttgcg attacaaaag cattgatcaa
     1441 agaacatgcg catgttgtca ttaatggctt tgcaacccat caggaagttg acgctctttt
     1501 ggcggagctt aatgggcttg gaaaaggcaa ggctgtttat tgtcaggctg atctcacgca
     1561 agtaggcgat attgaaaaac tgattcattt tgcccatgat acttttgggc gcgttgatat
     1621 tgttgtcaat aacgcgggca tgcaacatgt ggcccctatt acagaatttt cgcctcaaaa
     1681 atgggatcaa atcatcgccc ttaatctttc cgctgccttt cacacaagca ggcttgctct
     1741 tccgcttatg cgtgaaaatc attttggtcg tattattaat attgcgtcaa ctcacggact
     1801 tgtggcgtcc gagggcaagg cagcctatgt ggcatccaaa cacgggatct taggtctcac
     1861 aaaagtgatt gcccttgaga ccgctttaga aaatattacc tgcaatgccg tttgccctgg
     1921 atttgtcctc acacccctcg tcgaggcaca ggtgcgcgat aaaatgacaa agacaggcat
     1981 gacctttgat gaagcctcga aatctcttgt ctcggataaa caacccagtc agtgttttgt
     2041 tcctgtggag gaaattgcag cagcgtgtct ttatcttgct tccgatcatg cgtctcaagt
     2101 tcgaggagcg catctcgttg ttgacggagg gtggacaata cagtaaagta atttgttaat
     2161 ataaaaacca gaaaacttgt aaaatgaatt ttaaaattat ctcacttatt atcgcattaa
     2221 cgaatataac atcactctac gctgcagaaa aaaggtattt tgaggaagca gagtactatc
     2281 taaaagaata tcagacaaaa cttaatgatt ttgtgaatac atacgaaaaa gatgaaaaaa
     2341 aacttaatga gaaaaaatca agtctcacaa cttttctgtt cctcaatatt cttgagcctt
     2401 atgaaaaact cacagaaaat aaacgcaaag aactgaacga taaatatctc aaagatttca
     2461 atgacactga gaaaaagaca ttcggtgaaa gcattatcat taaaaaattc gaagagcatt
     2521 ttaatgaaca taaaaataag tccattatga cagagaaaag ctgggtagag cttaaaacgg
     2581 gtcaagacga aattatgacg atcgaaaatc tttctgagga aaataatgaa aagttaataa
     2641 ttttaaaaaa agagagtttt tcaaacgatt ttctccaaaa taataatatt attcctgttg
     2701 aaagtgaaaa aaacatcatt atggaaaagg ataatgatga agatgagact tttataaaaa
     2761 agaattttga agaaattaac agtaataaca gcagtaataa tagtagcaac aacagtatta
     2821 gcaaggaaaa aattaaaaat gtagaagatg agttcggaaa gaatcaaaac aatcttctca
     2881 atgacaaaaa taatgacact ctcaataaga ttgaagaaaa taaaaacatc attgagctac
     2941 ctaaaaataa taaaaataat aagcgtattt ctataaaaaa attcacagag gggtttaaaa
     3001 accttcttaa taataataaa aataaaagaa aaagctttaa tgagaaagac aaaaaaaact
     3061 agtgagattt tgagggcgtt tgttcctcaa aaataatcac ttcatccgcg cgggcaaaat
     3121 tcaaaacggc ccgcgctctt tcctccagca tatcaagatc caaactatct ggacgcaaaa
     3181 gatacacacg gcgctccaaa acctctttat ttccctgcaa agtattgaat tctaaacgca
     3241 cctgatccaa cttttgcttc atgcgcacca tggacaaaag accacgatcc ccctggatac
     3301 tgtgatatat aaaatacgtc acgcacgcaa acgcaataag cgggcccata aggccctgga
     3361 tctgaggatt ataaaaaaaa gataaaatcc ccattccttt ttgacgtcga acacgcatca
     3421 tttatccttt gttaaaaaga gtgtgggcga actgtgcttg acctgaaaga ttttcagcaa
     3481 tgcgaaggag ttgattatat tttgcggtgc gctcgcccct gcaaagagag cccgttttaa
     3541 tttgccccgc attcacagcc acggcaagat ctgcaataaa ggtatcctct gtctcgcccg
     3601 aacgatgaga aataatggat ttatatccgg cacggtgcgc tttttcgatg acgtctaagg
     3661 tttctgtcac ggtgccaatt tgattcagtt taattaaaat tgcattcgca gcaccctgtg
     3721 caataccttg actcaaacgc gttttattgg tcacaaacaa gtcatcaccg acgatttgaa
     3781 gccggttccc aagagctttt gtcatggccg taaacccttt aaaatcttcc tgatccaacg
     3841 ggtcctctat cgacaaaatg gggtaatgat ccacaaggtt tgtatagatc gatagcatcg
     3901 acgcgctgtc gtgctcagcc ccttcataac ggtaaaggcc cttcgcttca tcataaaatt
     3961 cactcgccgc cacatccatg gcaagggcaa tatgctctcc aggtgtgagg cccgcttcaa
     4021 caatcgcttg catcatcaga tcaagtgcag cttttgtgcc tttgagcgtg ggggctaccc
     4081 caccttcatc gcccacagct gtggatattc cttgtttttt taaaatcgct ttcagtgcgt
     4141 gaaaaacggc tgcagacaac tcgagcgcct ccataaaggt gcatgcgccc acaggaacaa
     4201 tcataaactc ttgcacgtcg agaccattat ttgcgtgagc tccgccatta ataatattaa
     4261 tcaatggcat gggaagcgtg tgcgcattcc caccccctaa atagcgataa agaggcatct
     4321 gataagcctt tgccgctgct tttgcacatg ccatactcac ggccaatgtt ccatttgcgc
     4381 caaggtgact tttattatct gtgccatcaa gatcacatag catgctatca atagcgcgct
     4441 gttgatcggc cgcgtatcct tgaagatgtt gaaaaatatc cgtctcaatc atttgcacaa
     4501 cgcgcttaac acctttgccc tgaaagcgat gaaaatcacc atcccgcagt tcaagcgcct
     4561 caaattttcc cgtggaagcg ccagaaggca caaccgcacg gccgcgcaca ccgtttgcca
     4621 gaatcacgtc gacttcgacg gttggaaatc cgcgggaatc caaaatttct ctcgctaaaa
     4681 tatttgaaat ggtcaaaggc atttttcatc tttctttaat gttctgtccg tcatattaaa
     4741 tcttttttgc ggttgttaat attctttccg tgtcaaacga agataatttt tcaccgttta
     4801 tatccgtttg ttctaaaatt gtaaaaccat tttcacttaa catccctctt aattcatctg
     4861 cggtatagca ttggagtgta tttgtatcct ttgagatttt tacaggttgg cgatggtgcg
     4921 tttgctcaaa atatgtggaa taagaggcaa gaatcccctc atacgttaca taactaaatt
     4981 gaacttcgcg tacagttctc tctttcaaaa tggcgagcct gtccaaggta agctttgtga
     5041 tattgtccgt gtgcttcaga tagttcagat taaatatatc aaaaacgtag atcccacctt
     5101 ttttaagatt accatgaata ttcagcatag ctttttaaaa accatcgcgt gttaagtggc
     5161 caatcgcatt aaaaattgta agaatagcat caaattgacc aacatggata taacgacaat
     5221 ctccctttaa aaactgaaca gtaacctttt tttgcattgc ttt
//
LOCUS       DAIAHG010000081         4897 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_81_length_4897_cov_3.837967, whole genome shotgun sequence.
ACCESSION   DAIAHG010000081 DAIAHG010000000
VERSION     DAIAHG010000081.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81_length_4897_cov_3.837967"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1449 a         1092 c          921 g         1435 t
ORIGIN      
        1 ttcgatgatg gcaacgtgga ttttaacaat tctcctgatc acggccctct atactgccta
       61 tcacatgacg caagcgcttc cctttgatcg tctccaaagc gagatgaatc tgctacagcc
      121 ctcaaatcct ctttccaaga aagtgattca ggttacaggt ggctattttt ctaactttga
      181 aacctatcga tggatgctta ttgcgccagc actcgctttt ggaggtattg ttttttcgca
      241 gttgtgcctt ttgctgcgcc aaagaggcct cagttttgcc ttaaatgccc tgagtattgc
      301 tggaattgta gctattccag gcctctctat gtttcccttt attctcccct cgtcttccca
      361 tcctgaccat ggtttgacga tttttgacgc ctcgagcagt catttgaccc tttttgtgat
      421 gacgattgct gcgattattt ttattcctat tattttagcg tatacggctt gggtgcatcg
      481 cattctgcgt ggcaagattt tagccaaaac gattgaatct caaagtgatc attattatta
      541 atcaacatta tttttcaaac taagcctcaa aaaggagtgt gttcatgtgg tattttagtt
      601 ggattcttgg cctgggtctt gcttgttttt tttccattct caacgccatg tggtttgaag
      661 tcatggaggc cgagaaaaga aatagagatt aatcaatcac cttctcaagc cacgaaagaa
      721 tatctgccac aacctttttg tgcgcttgat tcaaggaaag cggaaccgta acagctttat
      781 catctggaat cgaaacagcc tgagagaaaa gttttgaatt taaggatttt cggtcactca
      841 acgagaacac ttgtacaaaa tacgtcgcca cagccttgtg atcgcaacag cgcctaaaaa
      901 actctctcac atctaccgtc actaaatatt tcgcttgaag gcctgtatta aggcgcccta
      961 cagactcaaa aagtgctgca ttttgaaaac tttggatcaa actttcatgc accaacacac
     1021 tcaatcgatc cccccactca atgtccgcaa tataatcaat cgtattggct gtgggcaaaa
     1081 gcgcaatgcg cgtattatcc agaggtgtgt acacagaagg cgtatcaata ataatcgccg
     1141 atttttttgc gcgtttagat gcagaagaag gtgggatcga ctccagcaaa aagcgatccg
     1201 gtatatcatt gggctctggc agaagagagc agctactgag taaaaacata cccacgataa
     1261 agaaccaaga ttttgtaaat ttttttaaca tgagggtcat tttaaacgta ctccacgatc
     1321 aggatcatta tgtaaaaaac gtgttggact acgctcaatg gcttcaataa cacgtttaaa
     1381 agcagcaaag gttacttgtg cctcgcctaa aaacttcgtg agtgttgtga gtcccgctcc
     1441 tgtaaaagct ttgagcccct cacgattttc tgcaaaaacc aggcgaattt ccttcgccgc
     1501 tccatgaatt tcgtcaagag tattgttgag tttctcaaag gctttgacaa tatcttcgcc
     1561 aattgtgtgg ccccccttac catcttttga tttaagggca tctgtcaatt cttttacgtt
     1621 aaataaaatt tctttaaaag cctcacgatt ttcctcgctc acaacatttt taatatccgt
     1681 tgtcaacgct gacacttcat ttaaaagctc aggcgcgtta tcaatcactt tatcgattga
     1741 agaatctctc cctacaattt gtggaatatt tttctgacta aagcgaatgt taggtgcttt
     1801 atcacttccg ccagtcagct gaataaacga tgttcccgta attccttgaa tatccacaga
     1861 ggccaccaca tccgttttca aaggaatatc ctcatcaata ctcacgttca caagcagttg
     1921 tttaggatga agcggattca ctttaattgt cctgacactt ccgatatgaa gaccttgaaa
     1981 gcgcacaggg cttccctcat tgagccctga aacttttgaa aaaacaatat ccaatgattt
     2041 tgctttatga aaaagattaa cgccagaaat ccagatcaga aaggctataa taaaaagaat
     2101 aaagcctaaa acaaaagcac caacgatttt ataatttgcg tttgtttcca tcagacatcc
     2161 tttttcactg tctgtccaaa gagtctacgc cctctatccc cataaaaata ggcatcaagc
     2221 caggggtgat gctgtgtatt catatgcttt ttgtccatca caataagttt ttgatccaca
     2281 attacagcga tgcgatcaca cactttcgcc aatgtatcaa gatcatgtgt caccataata
     2341 acagttaaat taaaattttt ctttaatttt aaaattaatg tgtcaaaatc acttgctgaa
     2401 ataggatcaa gccctgctgt gggctcatct aaaaaaagaa gcggtggatc caaaatcaac
     2461 gcgcgtgcaa taccaacacg tttcaccatc ccgccagaga tcaatgaggg atatttttcg
     2521 ccgtcactct catccaagcc cacagttttg agttttaaaa gcgcgagttc tcttgccaca
     2581 gtttcattga cgtgtgccgt tttcacaaga ggcaacataa tattttcaag aactgtgagg
     2641 gatgaaataa gcccgccact ttgaaaaaga acaccaatat tatttcttga aaaaggagga
     2701 tgatacgtaa tttttccttt ttgtggttta tgaaggccca tcatgtattt cagcaaaacg
     2761 gattttccag acccggatcc gcccacaatc cctaaaattt ctcctgcaaa cacatcaagg
     2821 cttatatcct cgtgaatgtg ctgctggcca aattgcgttg ttaaatgctg aacactgagg
     2881 caaagatgca tgcgctttaa acctttaaat aggaaaaaac aagcgacatt aatgcattcg
     2941 ccacaatcac aagaaaaata gcctcaacca cagaagctgt cgtcattttt ccaacacttt
     3001 ccgcactccc acgcacttgc atccccctaa agcaccccac aagaccgata agaagtgaaa
     3061 aaagaggcgc tttactcatc cccacccaaa aattattgag cgaaaaagcc ttttgaaggt
     3121 aattaataaa ttgtgtcggt gtcaaatcaa tcacaagata cgttgttagc atgccgccaa
     3181 tgagtgccat catatcagag agaaacacaa gcagaggaag tgctataaga agtgctatca
     3241 tccttggtaa aacaagataa attacaggat cgagttgcat catccgcatc gcatcaattt
     3301 cttgatttaa actcatcgtt cctatttgtg ctgtaaaaga acttgcggat cttccagcca
     3361 caaccaccgc cgtaaggagc acgccaatct cgcggaaaac accaatggtg agaaaatcga
     3421 tcgtgtaaat ctctgcgcca aaccgcgcga gctgattaat cccttgataa gaaagcacca
     3481 tgccaatcag aaatgaaatg agcgcaataa tcggtgttgc ctgaaatcct acttgatcaa
     3541 gatgatacac aaaggacgtg aggggaattt tccgatgacg tctaaaagtt tcataaagcc
     3601 tgacaatcac atacccaaaa aacgttaaaa gctctgtagc tgtttggcca aatcttgagg
     3661 cacttttgcc aatgagagct aaaagggatg aaaaagaaaa atgattttga gatagaggct
     3721 caggcaacgt ctttttctga gccatttgtt cttttatttt tgcctgaata agacggctct
     3781 gttctgtccc atcaaaagca acatgatctt gtttttcata gacaagaata agaacagcaa
     3841 tagcccgtgc acaggcgtcg ctgatctgac accgcgcatc aaattgaatc gataaagact
     3901 cattggtttt aagcttttcc tcaaggccaa tcccccaccc ctgataatga cgcatatcca
     3961 ggggctcaat aaaccttagt gtatttttgt gaaagagatg tgggacggaa accatcgtgc
     4021 attaaattcc acaagcaaaa ttgttatttt ttctaaaacg gattctacct agaattatcc
     4081 ttcctcacgg taggattttt ctttgcgctc atgacgctct tgagcctcaa tacaaagtgt
     4141 tgcaatcggt cgtgcttcaa gacgacgaag accaatgggc tcccccgttt cctcgcaata
     4201 cccatacgag ccatcctcga cacgatgcaa agcctcatca attttattaa taagtttccg
     4261 cgcacgatcc cttgctctca actcaagatt acgctcggtc tcattcgccg caagatcaag
     4321 aatatcaggt tccgcgtgac tttcttcttg aaggtggtga atggtttcga ggctgtcgtg
     4381 aaggagttgg tgtttccact ccaagagttt gcttttaaag taggccagct gccgtgggtt
     4441 cataaagggt tcgtcattgt gagggcgata gtcggggtca agtgaaaaag tctcttctgc
     4501 aatcataaac agggagttcc ttttttcctg ttaacaatag cgttagggtg cgataaaaaa
     4561 gttaacaagt cattaaaaat ttaacaaaat ttttaatttt ctggggataa gtcccctttt
     4621 ttcaacaaaa gcagtcgatt tcatcacaga aaaaagcttt ttcatcctgt taatgatgaa
     4681 atttttgtcg tttcagctca aaacacatta tcctcatcaa ttttttttga ggactctctt
     4741 cgtgaaaaat aattataatt ttaaacacat agaaactttt ttcctttatc gtgaatgatg
     4801 ggaaagtttt tgaaccgaaa gtgtccgtga tgaaaattct gtttttattc gcattgattt
     4861 tgtctttttc ctccatccaa ggcagtgatc tagagga
//
LOCUS       DAIAHG010000082         4864 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_82_length_4864_cov_3.415918, whole genome shotgun sequence.
ACCESSION   DAIAHG010000082 DAIAHG010000000
VERSION     DAIAHG010000082.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82_length_4864_cov_3.415918"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1535 a          859 c         1031 g         1439 t
ORIGIN      
        1 cgattcaaga aaacaaacct gagggtgtga aggattattt tgaaaaatat cccatggctc
       61 tttatgattt accgccttcg aatgatgaaa aaacttttgt tgatgaagcc atcgattcaa
      121 agtcttttga ggctcttttt gaaattttag agagttatgc gggggtgccc tatcattcta
      181 ttggcgctca ggaacggttt aatgagtggt taaaggtttt aagcaatccc agcgtacccc
      241 ttgaagtaat caaaaaagct tgggaaattt ttatctcctt aaaaaaacat gtctatccta
      301 tcgtgggtgg gaatgaggtg atatctggta aacccattta tgaagaatta cttgatgata
      361 atcatgaaaa aattatcact cacatgtgtc aatccaatca taaaactgag gatattcaaa
      421 aaaaatgtaa tgcgtcgatt gcgtttatgt tgcgcatgat aggtgagaaa aatgctattg
      481 aaaaggcagc ttttctttat gcatctttgg attggtttga gcggaatcaa tcaaatatta
      541 aagaatattt ttataaagat agtctatcca gcacaatcat gcttttgaca aaagatatct
      601 taaaagcatt tgacgttgat gaagcgaagg cacgcttgaa acttcttttt gacagtgcaa
      661 caataaattc tggttcagac aaatattctt tagatcaaac ccaaaaaatg atcatagaaa
      721 acaaaaatct ctcaaaggac aaggatttca ttgacttcat caagaaaaaa atcgatgcgt
      781 ggagaaaaga actagcatcg gatgattaaa aaaagtcggg ggggggggtt aaatcccccc
      841 atctcatttt ttactattgc ccgaccacac gaagagaacg gttaacctgt ggtgcaatca
      901 taggatcttc aatgggaatc ccaggttcat ttttcgattg gcgcatgaca agaaaggtgc
      961 gaatctgatt aacggatgga tagcgcccta aaatagttgc atgaaaggac tgataatcat
     1021 cccagtcctt ggcgtaaatt ttaagaaaat agtccgtgcc gcccgacata agataacatt
     1081 ctctgacttc attccatgtt ttaacagcgg cttcaaagtc gcgcaaatca acatcatttt
     1141 ggctcttaag agagacttgc gcaaaaatta aaacggaata tcccatggaa atggggtcaa
     1201 taaccgcatt ataaccttta ataatgcctg catcttcgag ctgacgcaca cggcgtaaac
     1261 aaggaggcgc tgagatatta caacgattag caagttcgac atttgtgata cgaccatctt
     1321 cttgaagatc atggagaata cgacgatcaa tgtcatcaag tttcattatt tgttttgtcc
     1381 aaaattataa ataatattct tataacatta tgtcgcctat gcgtggaaga aaaatatccc
     1441 agaaaaaaga aaaattttat aaaaaattaa cactttctta ggaattagat ctctatgctg
     1501 acttaaaagg gctaatttat tttaaaaaag aggaagctat ggcagatgaa aaaaaagata
     1561 atcagacaac ggtgaaggat gctgagattg tcgggagtga aaagaaaaaa tccaataaaa
     1621 tgatgtttat tatcattcct gtcctcgtta ttttagggat cacagcaggt attttccttg
     1681 gtcctcttgt caaatcgatg ttcggtggca aagaaaatca tgggaaagag ggggaatctt
     1741 ctgagaaaaa agatgaaaaa tcttcggagg atggcgatag tgatacagat agtatgggga
     1801 gaaaaaagcc tccgattgat ccaaaacttc tcacatttat ccccatacca gatgttttgg
     1861 tcaatctaaa aacagctaaa aatgcacgcc ctattttttt aaaagtgggt attgagcttg
     1921 aacttcacaa tcctaaaatt aaagaagaca tcgaagccct taaacctaaa gtgatcgatc
     1981 agatgcagct ttttttaagg gatttagatg ttgcggatgt gacaggtgca aataatttgc
     2041 agcgcgtgcg tcgtgagctt tatatgcgga ttaataacgt cacatcgccc tataaggtgg
     2101 atgacgttct tataaaggac tttttaatcc aatgacagac gatacagaga tctcaaatcc
     2161 aggggatggg gaagaaaaag catcttttga gagctcttca atggagagtg cagaagcatc
     2221 aaatgctgaa gcgagcgatg aatccaatga tgatggggat gatttagcgg ctgcgtggga
     2281 ggaagcgctt gatactcatg gtgctgatca tcttgccctt gatgtagata ttgaagcgga
     2341 taaaattctt tctcaaaatg agattgataa tcttcttggg atcgaatcag atgcctctgg
     2401 cgcatcgaaa ggcggtattt tccagattct taatagtgaa aatttacact atgaacgtct
     2461 gccgatgttg gaggttgttt ttgaccgcct agtgcgcctt ctttcaacaa gtttgcgtaa
     2521 ttttacatca gaaaatgttg aggtttccct tgaatctttt gtatcactcc gttttggaga
     2581 ctacatgaat tctattccgt tgcctgcaat gattaatatt tttaaagcgc ggcagtggaa
     2641 tgattttggc attatcacaa cagatagcgc gcttatttat tcgatcgtcg atgttctttt
     2701 aggagggcgc aaaggcaatg ctctcacgcg tatcgagggc cgtgcctata caacgatcga
     2761 gcgtaaactc attgaacgcc ttgtggaagt tattttagct gacttggagc agtcctttga
     2821 acctctttgt cctattaatt ttgattatga tcgcctagag acaaatcctg cttttgctgc
     2881 gattacacgt cccattaatg cagtgattgt cgctaaaatt cgtgtggata tggaggagcg
     2941 tggtggaaaa attgagttta ttattcctta tgcgacggtt gaaccgattc gtgatttgct
     3001 tttgcaaacg tttatgggcg aaaaatttgg ccaagattct atttgggaaa atcactttgc
     3061 aggacagctt tggacaacgg attttaccat gaaggcgatt ttagatgatc tttatctgcc
     3121 tctcggggat gttttaaaaa tgaaagtagg ctctcaaatt atgttgaatg tgaatcccga
     3181 gtcgccagct cttctaaaat gcggcgataa aacacttttt aaagggagta tggggcgtaa
     3241 agggcagcgt attgccatta aaattgatga ggaattttta cacaacgatg gaaaggagat
     3301 gcactcatga cgcttatgtt tttagacggt atgctcgtta ttttattgct ttttgggatt
     3361 aaggccgtgc gtgagtttta tgataaatac aaagagttta tcagtcttaa aaatgatctt
     3421 aattgtattt taaggcatgt gcagctctcg atgaagtccg ctcaagggac aattgatacg
     3481 cttcaaacaa atataaaatt tgcagctgaa aatatcacac cttttattcc agaagcgaat
     3541 acagcgaagg atgatttaaa atttcttatt gaaaatgcag aggtcgtgtg tgatcgccta
     3601 gaaaatctca cgcgggagat gaaaaaaaat tatttaacgc ctcagaccgt tgtttttgat
     3661 cccaaagata ccgagcataa aatttctaca acactcagag ccgaaagaac tgaacctcac
     3721 acccctgata ttaagccaga gcatagaggg tttttttcaa ccatctcaag agcccgttaa
     3781 attatgggat tttttgtgcg aaaaaaaaca aaagaaattt cgtcaaagca atgcagcgtg
     3841 gatcgtattc atccgggtga ttttaaggtt ttttgtgcac ttgaaacaca atcgaaggat
     3901 gttcctcggg aaaaactaag cgctcatcaa cgtctacagt atctgagcat gattatcctt
     3961 ttcatcatcg ctcttgggac tcattcatgg ctttttatcg agcagcattt tgtgaaaaca
     4021 attatgtccc ccattatctt acttctttcg ggagctgtgt atgatcaggc gtatggctct
     4081 caaaatcctc ctcaaaatcc taacaataca gctcctgcta ttcccacaag tacatctgca
     4141 cctgtcccac ctgaggacaa aaaaaaggag gaagatgttt ttgatcctct gagtcttgat
     4201 gaaacgcgtg ttaaaattct tttgtctttg aatgaccgag atattgagct taaaaaacgc
     4261 gaagccgctc ttcaggaaaa agaaagcatg ataaaggtcg aggaagttca actggataaa
     4321 aaacgcacag agcttttgga gatgaaaaag gttgttgaaa atattgctca gggaaataat
     4381 gatgaggttc ttaagaatat taaaaaaatg atcgctgttt atgaggccat gaagccccaa
     4441 gcggcagcgc gtatttttaa tgagcttcct ttgcaggtgt tgacggatct tatcaagcat
     4501 atgaatccta gaaaatcatc aagtattatt gctcttatgg atgttggaaa ggcaaaggca
     4561 cttacggata atgtggcttt tgcgccctat gtgccagagt cttcaggaaa ataggagtta
     4621 attatttgac gtatattata aagagttgat gctatgcgtc tcatcacaac atccacgacg
     4681 catcatttct caaaagtatt cgcctcaaag gtcgatcatc ttcattttca gacttatcca
     4741 agtggtgagc tttatgcggg taaaattaaa aattatcagg gcgaaaagat tattattttg
     4801 catgcaacgc caagagaaac gcttcatcgc tctttttttg agcttttctt tatcctggaa
     4861 gtgc
//
LOCUS       DAIAHG010000083         4682 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_83_length_4682_cov_3.147448, whole genome shotgun sequence.
ACCESSION   DAIAHG010000083 DAIAHG010000000
VERSION     DAIAHG010000083.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83_length_4682_cov_3.147448"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1321 a         1010 c          931 g         1420 t
ORIGIN      
        1 aaggaaaacg taaagtcctt ttcgagagcc aagatcctgg ggcgtatatt tgccatttca
       61 cggacgtcgt tcatccagat cttccgattg tttccggaaa aggggttatt aataatcgta
      121 tctccgagct ttttttcgcc cgcttaaatc agctcaacat tccaacgcat tttatacgtc
      181 gcctgaacat gcgcgagcaa ctgattagag ctactgatcc ttttcctttt aaaattaaaa
      241 tgcataccct tgcgaacaac gatcttagcg agcgtttttg tctagaaaaa ggaacaccac
      301 tgccgcgaac ccttttcgaa tattttatga aatcttcgtc gaatcctttg atgagccgtg
      361 agcatatcat gacgtttgga tggagcaacg agatagagct ggacgatatg acggaaattg
      421 caaggcgcgt tcatgatttt ctctatggct attttatggc agtaggccta cggcttgtga
      481 attttacact cgaatttggt cgcctctata gcggtgaatt tatggaacag tctgagcttc
      541 ttatgattga tgagctgagc ccggatacaa tcgagcttca agatgtcgca acgggagatt
      601 ttctttcact caatatggtt gaaacagatc ttgcaacagt cgccgccacc tatcagcata
      661 ttgcagagcg cttgaaagtg ctgcatgctg tgaattcgtc aagcactcct ttgagtgtgg
      721 gttagtaaag gtatttatgg cgatccctgc aacgcaatta gagaaaatga ttaaaactgc
      781 ttttccaacg gcagtagttg aaattaaagc gcttgttgat gacaatgatc attttcacgt
      841 gacgattaaa gcaaaagagt ttgaggggaa atcacgcctt caacagcatc aaatggtgta
      901 tgaagccctt ggggctacga tgaaggcaga tttgcacgct ctttcaatca aaacagaagt
      961 gcttaaaaat aattcagact aacgatgtgg agtaataaaa tgacgattca tgcagccatt
     1021 gatgaacttg tgaaaaataa tgacgttgtg ctttttatga agggcacggc gcaactgcca
     1081 caatgtggtt tctcctctgt ggttgttcaa gttcttggca aacttggtgt ttcttttaag
     1141 gatattaatg tccttgagag tcccgaaatt cgccaaggca ttaaggatta tacgaactgg
     1201 cccacgatcc ctcaacttta tatcaaaggt gagtttattg gtgggtgtga tattgtgcgc
     1261 gaaatgtacc aagcgggtga gcttcaagaa ctccttcaaa aacatgccct tatggctgcg
     1321 taagagtgtg atacacccta ccgtaagtcg taaggatttt taggaaacag tcatgacgat
     1381 tattgctgtg gatcagggat atcatttttg tgctttggcg cttaaaagta aaaacaaagt
     1441 ctttaagcgt actcttacac tcgatacaaa aaaactcaca agcacgctta ccggaacgtt
     1501 tgttcaagag ctgcagcagt tgtgcagtga ggcagacatt gcactgggtg atatgcgttt
     1561 cattgcagcc acggtaggac cttcttcttt tacagggttg cgcgtgaccc ttgctgccct
     1621 tcaaggcatt gcttttggta atcaagccaa ggtttttgct tgtacacgtc ttgctcttca
     1681 ggcctttaaa gcttttcaaa acactccaga aatgacgcac tatacgagct ttttaagcaa
     1741 tcaacgggga gctttttttt ctcaggagtt tagccaacaa aatagtctcg tcaaagaaat
     1801 ttcacccgtc tccttagtgt cttttgcgga ctatcctcaa aaaaactatg acgaagatac
     1861 ggatacggcc tctgacctta tagcttttgc agaaaaaata agtgaaaatg cttggggaga
     1921 ttatacaaac cttgtgccct tttatggcca cgatccagtt tttaagagga aaggtgatta
     1981 aatatcatcc tctcctctca agattaaaaa attatttttt ctcgccagga agtgctctct
     2041 ctaaaaacac ctcaaagggt ttgagatttt catcactcag accaaaggtt tccttcagtg
     2101 ctgtgataag ttttttagtc tcatcctttg cattttcttt tgagagattg agattggctt
     2161 gaatagctcc cgttttgggc catggtgttt tattttcaac atcctttgca ttaaaagatg
     2221 atgaaacaac ggccgcaggg ttactgaggc gtttataaaa atcttgaata agtttttcta
     2281 aggtttcatt attctctggg aatttactta tattaatata agcggtaaga gtcgttttag
     2341 ggttttcgca ccctgtttga aggatgtcca tgttcattcc gtcaacacca taaggaccac
     2401 ttgccggaaa tgatgttttt tcatcggatt gctggcgaca agattcaaca tccgtgatgc
     2461 gttcttctcc tgcgggtgtt gtaatgacct gcgtatgtgt ttcggcaaag gcgagtgttg
     2521 tgaaggtgct tataaaaaaa ataggcttta acatgaatca ttcctttttt acattgattg
     2581 acacattaag gttagcattt attttttaac tttcaagccc tcttatttaa aaaaaatatg
     2641 tcttaagcgt ttcttggatc tttttttctt ccatggatgc gtcaactaca acacagcgct
     2701 tcggtgcatt ttttgcaatt gttaaaaaac ctgcacgcac tttttcatga aaattaagat
     2761 cgcggtcatc aatatgatta ttatgactgc gtaacgcaag acgcgctctt gcaatcagag
     2821 gatcaagatc aaaaataaag gtaagatccg gcactcgagt aaccgcataa tgttgaagtg
     2881 tttttaaaaa ctctaaggac aaacccaacc catagccctg atagaccatt gtggaatcca
     2941 taaagcgatc acataacaca atttttccct catttaacgc gggttctatc acttttttgc
     3001 aatgttcata acggcttgca ttaaaaagaa aagcttctgt gataggctcc catgtgtgtt
     3061 ccaaaagaag agagcgaatg cgctcgcaat tttcgtctcc ccccggttct cgtgttaaaa
     3121 caactgattt tcctttttct tctaaatatt ttttaagcaa gtggatctgt gttgttttcc
     3181 ctgttgcctc accaccttca aaggtaataa acatgaatgc ttcctcttct tatgcgtgat
     3241 gatcttgagg gcctggccag aaaaaaggat taaacgcaat ttcccacaca tgatcatcaa
     3301 gatcagaaaa ataaccagaa aaaccacccc actctgtttt ttgagggacc ttaaggcact
     3361 gagcccccgc ctgctttgcc gcgtcaaaaa gcgctaacac ttcctcctca tttccaacat
     3421 tatgggaaaa agtgacacct ctaaaaccac tcccttttgg atcaatcgtt gcatcagccg
     3481 caagtaaatc ccagggataa agcgcaagcc aggtgctact cagtgtgaaa aaagtgatat
     3541 cgccctctgc catttttaat tgtggaaaac caaggccttg tgcgtaaaaa tccgtagcac
     3601 gcctaaggtt tttagtcccc agtgtgatca tgctgatccg tggctgcata aaatatctcc
     3661 ccccttttaa tgaataatca gagcatccga aagagggcgc tccatcacgg tgccttctcc
     3721 ctcatcacag tcccctaccg tgtgattcac aactttccct tcaatccaca aagtggatga
     3781 gccccctccg tctaaattaa gtgctgtcac gcatccaagt ttttgcatga atgttgcaag
     3841 ctctaaaagt gtaagcccct ttgggcctgt ctttgatgta aattcttctt tgaggaattt
     3901 tttagtctca gccaacgtca atgtctttgc attttttccc ggatgtgttt tagaaaattc
     3961 atccacaaag gcctgaacat cccccatagt catggctgtg atatctttta aatagccatg
     4021 ttctgcgaca acaatcacaa catctccgtt agattttatc cccacagctg tacgtgcatg
     4081 gggttttgtg taaaaatcac ttatttgttt tttaagagcc tcaacgacct ccccgtgttc
     4141 caaaagaagg ggaatccctg cgaccataga gaccgtttga gaaagataat ccttagggtt
     4201 cccttcaata atagaaagag tatcttctgt taaaataaga agcggctgct ttttttgccg
     4261 gattgtataa atttttccct taatcacaag tgttaggctc gcttcaccgt ctttcttgcc
     4321 acctatttca aaaaaccccg cattcacagc aacttttgct tttgtgcggg ctgccataga
     4381 tgaaagcgtc tcgcgccctt ttccatcatt tgctttaaca agatcgaaag tattctctcc
     4441 ctttttaagc gttacaacat gaataatatg cgaaccttct gtatatttct gatagacata
     4501 accaaacgtg cttgaaagac aaagaccata aatcaaaagg aaaaaataaa acgcaccaga
     4561 aaatactctc ataatcttaa gcttttcccc ataagagata ggttattgag cggaaaaatc
     4621 gtgtgagaaa attaactttt tcaacatctt cacccgctgc aagagcgact tcatgctgag
     4681 tc
//
LOCUS       DAIAHG010000084         4614 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_84_length_4614_cov_3.268459, whole genome shotgun sequence.
ACCESSION   DAIAHG010000084 DAIAHG010000000
VERSION     DAIAHG010000084.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84_length_4614_cov_3.268459"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1335 a          963 c         1078 g         1238 t
ORIGIN      
        1 atatgcaaag tgagtgaagg ccacaaaaat tttgagggct atttttcgta gagtcctttg
       61 agcaattttc caagcgtttt aaccagattt ttctggtcat tgccttcgag tgtattaaaa
      121 aaatcgcgat caatcccttc tacaatgggg acaagcaaat tcaccatttc tttccctttt
      181 tcggtgagtg agacgctctt ggcgcgtgta tcttttgtat ggggtatgcg ggcaacataa
      241 ccaaaatcta tgagtttttt gagggactgg gagacggtca ttttgtcaag cttcgtccat
      301 tgtacaatca ggatttgtgt tgtatcagag tgatgggcct caaaccacat cagggttgcc
      361 ataatcacaa actgagcctg ggaaagggtg taatcatcca acgctttttt gatctggcgc
      421 tgccaaagca ttgtcgtttg ccagagcaaa aagccggggc tgtcttcagg atcctcaaag
      481 ccaaaaggtg tattagacat tgaattgcct tgctttttta atcagcgcct ctgtttcagc
      541 tggaatagta ttggcaacat tttgagccac aagcttgatc cagagccatt tgagtgggcc
      601 tttgacaaca agtgtgttgg tgagttttaa gccttctgca gtctcttcca tagaatgagt
      661 gtcatacatt ttggcgccga aaaaggatgt gcaatccgta aatgagtagc cctcatgaac
      721 ctcgacaagt tttatcttaa aagttttcat gcccttgggg gtgaggaaaa agtagcttcc
      781 cacctcaaag tctccctcta aggtgcacga ggccaggtca tcatgccagg ttggccagtg
      841 actcatatct gtccaaatgc gccagatatc ttcacgttta acaccgtgat atgtttgggt
      901 ataggttctt gtccacataa gcgatttcgt ataacttttt actatatact attatattat
      961 atgtgttttt actaaatagt caagcgctaa gcaacagaaa attgagcata acacattaaa
     1021 attctcgctc tgtgggggta tgcgcgcatc gaagcctcaa caattaacct caagacatgt
     1081 tttgcaaaaa aaaaccagta aaatcaaggg ttctgggcat gcgtcccttc gggtcggacg
     1141 ttcgcatgta tcggatggag agcgtaaaaa caggagaaag tgaggtacgg gtctaccaca
     1201 aacgcttttt agcaaatgca cgaccggaac aagacttcaa atatttttca aattgccttg
     1261 ctttagcctc atttttaaag cttattatac aattaattcc catggtttgt tagctgtatg
     1321 cacagaacca ccagaattgt gagtttctag gcgtttttta aggtcttcag taatcccaac
     1381 ataaacttgg tctgaaaaag aaattgagcg aactaaataa acataaaaca taatagtcct
     1441 ccttcgccaa ggcttcggcg gacagctttc gttaacataa atctggcctg tcagctgtag
     1501 ttcgcagaac gaaagctggc ggagagagag ggattcgaac cctcgatacg gtttaacccg
     1561 tatgacggtt tagcaaaccg ttgccttaag ccgctcggcc acctctccgg taggtctagg
     1621 attatcgttt tgcgcgtgaa aagtcaaggt gatttctcat ctatgcatgc aataaagcat
     1681 cagcgttttt tcatgcataa ttcgctcact aaaagtactc agggcaagga ggcgactcgc
     1741 atgccccatt ttttggcaca aagaataatt gtccaaaagc atttttatcg catgatacag
     1801 cgcatcgctg ttacgggacg gaatcaggag cccatttgtg cccgtgataa gacttttgca
     1861 ccctggtgcg tccgttgtaa tgatcgggag gccggtcgcc atggcctcta aaagactttt
     1921 gggcagccct tcgcggtagc ttgggaggag agcgatttga ctgttttgat aaatttcagc
     1981 aacatctgtg cgttcaccca gccactgaat aagcccctca ttgtgccatt tttgcaaggt
     2041 attttcagga acatgatcag ggttttgcag atccggcgcc ccaacaagtt gaatcgttgc
     2101 ttggggataa gctattttta agcgctttgc tgctgtgatc acttcaccaa tccctttgct
     2161 ccacagcatg cgcgcgacaa gcgttatcac atgattgtga ggaagggacg gggcgggtga
     2221 aaaattatcc gtgttcacgc caacgccctg gatgaggtga agcgaatcgg cagaatggcc
     2281 aagccatgtg cggatggtgg cttcgtcatc gggattttgg acaataataa cggttttttt
     2341 gagggagaat tttaaaaaag ggagtaaaat cgtgcgaatg atgcgacttt tgagggtatg
     2401 tgatgtaaaa acatagccta aacctgcaat tgcattgatt attttaggta tttttgtgat
     2461 ttttcctata aaacttccat ataaaatggg tttaagggcc acattatgaa gaatatcggg
     2521 cttaaaatgc ttaaaaacag aataaagcgc tttgaatgtg ggaatatcgt tgaaaggatt
     2581 aatccctgtg cgatggaagg gaacctcaaa aactttaatg cccaaggact caatgtggcg
     2641 tttgtcttca gaaaagtttt ttgaggaagt tgtcgccact gccacctcca tgccctgctt
     2701 ttgtgcagca agggcgagag agaggcggtg gctgataaaa tagtgggatg ctgcgacaac
     2761 aaaaagaaga cgggggggca tcgctcgatt tatacctttt tctttgcgtt gacgtttttt
     2821 acaaactgta cggcgccaag ggcaataaca gcggcacctg tgagataaaa cgcgggcacc
     2881 atgttcgatc ctgttttatc aatgagccaa gtcacgagca tcggtgccgt tcctccaaaa
     2941 agagggccac taatgtttgt tgtgagagcc gacgccgaat aacgcacgcg tgtgggaaac
     3001 gcttcgatca tcaacgctgg aatagggcca aagtaacctc caacagcgat ggctaaaata
     3061 agctgtgcga taaaaatatt ctctgtgctt ttcgtattta ccatgatcat gagcggaatt
     3121 gtaccgatga taaaaagaat cgcggccgcg ctgaggattt ttctccgtcc ccatttgtcg
     3181 gacaaccacg ccgaaaggac ggtgacaatg ctcaccacga caaggttgac cgtgttaatt
     3241 gtgaaggata tatcctctgc aatgccaagg acggaggtca ggtaagttgt catataaaca
     3301 aagagtaaat agaaactgag gtcatgaagc attgttgcgc caatcgcaaa tgtcagttgc
     3361 tttttatgtt tatgggcaat ttccttgagc ggatgagcga cgttgtcttt gtttttttgc
     3421 tcttccaaaa actcaggcga ttcagtgact tggcgacgca tataaaaacc gacaaagcaa
     3481 atgagcacgc ccattaaaaa aggaaggcgc catccaaaag agtgcagatc actttcgctc
     3541 ataatttttg agaaaaaagc agctgtggcc gatcccagca aaatacccgc aagacagctt
     3601 gccacagcaa aaccacccat caagccgcgt ttattggggg ccgtgtgttc tgtaataaag
     3661 gtaatggagc cactatagtt gcctcccatg gaaatacctt ggagcatgcg catggtcaaa
     3721 agcaaaatgg gtgcccaaat gcctacattg ttatatgtgg gcagcaaacc aataatcgct
     3781 gttggaatgg ccattaaaag aatcgcaagg acaagggcgc gcttgcggcc aaaaagatcc
     3841 ccaatatgac caaaaataag accaccaagg gggcgtacaa taaaacctgc agcaaagaca
     3901 ccaaaagctg agataagctc gaccgtggga ttatcagagg ggaaaaaaag tttgccaata
     3961 ataggggcga aatacccaaa aagggcaaaa tcataaaatt caaaaagatt tcccaccatg
     4021 ctggtagaaa tcgtgcgcaa aagggatggc ttcgacataa gataaaccta ctcgttttaa
     4081 tatgtttgta agatatataa ggatgttatg taaagaataa aagggatatt gctcggcctc
     4141 agccgagaag tcgaagggaa agagcaatgg tgaacaaatt tctcataaga cctttattat
     4201 gttgaatggc atgcatgaat gtcaagaagt ttgtgtcctg atcgattaaa aaaacgcaca
     4261 gtgaattttt ttctcttttc taggattttt catgcataaa gcctatatag tacattagaa
     4321 catcaactgt gccacgctat ctcttatgca acacatggag ctcacttctg atcaaccgtt
     4381 tcagctcgtg tcgtcttttt ctccgggagg ggatcagccg acggcgattg cccagcttac
     4441 acaaggtatt gtcgagggta agcgcgatca agtattactc ggtgcaacag gcacaggtaa
     4501 gacctttact attgcaaatg tgattcaaaa cacacaaaaa ccgacgctta ttatggcgcc
     4561 gaataaaatc cttgctgcac agctctatga cgaaatggtg ggtttttttc ccca
//
LOCUS       DAIAHG010000085         4612 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_85_length_4612_cov_3.417861, whole genome shotgun sequence.
ACCESSION   DAIAHG010000085 DAIAHG010000000
VERSION     DAIAHG010000085.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85_length_4612_cov_3.417861"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1346 a         1008 c          901 g         1357 t
ORIGIN      
        1 tcgatgtccg tgtggaaaat gtaattgaca tagtgccccc tcttcacgct cattatgtat
       61 ataacatcga tggcgtgtca attcaaggaa aagccgttat ctacgacgtt aaacctcgtt
      121 tcatcaaagc atttgtgtta aactttataa aatgacaggc ttttgatgcg cataatagat
      181 tcccatgaaa accctaatct ctaagaataa atcgtctcac ggaaaagatc agcatcttca
      241 taaaaaagcg cctaaaaaac cctttaaagg ccttgctaaa gagcaagagg aaagtggcct
      301 ctcaaaggct gaattttata agaaaaaatt taaaaaaaga aaaaaagacg accacgacgc
      361 atttttctca gaaaaaaccc aaaaaaaacc aagagcgagt ctatctgcaa gtcctttgca
      421 aaaaggtatg tttgtacacg tacgcatcaa agaaattctc agtgacacag gtaatattta
      481 tctttctgct cttgggaact atcaaggaaa agatatcttt attgagcgcg tacccgatac
      541 aataagtaca gatcaaacgg ttgttgtaaa attaggggcg cggcaaaaaa aactatggat
      601 gggtgactat attcgtcttc ttcgtgacga gacgcagctc cttggttttt ttgatgcaaa
      661 aacaaaaatg atttttccta ttcaccgtaa aaaagatttt acccccattc cgtgtacagc
      721 ttctctgccg gatcagatgg ttgttgtctt tgaggaaaaa gagggtgcgg ctcttatcaa
      781 ggaggaaatt ggtcttataa catctcccaa atcctatagc aaagtcgccg cctttaacca
      841 gggtgtgtgg aagttgatta cagaggaaga agaggccttt tgtaagaatt ttaaaattcc
      901 agaacttggc aaacgggatg acctccgctc ccttgatctt gtgacaattg atggtagcga
      961 tgctaaagat tttgatgatg ccgtgtgggc agaaccggat ccagaccccc gtaatcctgg
     1021 aggctaccgc attgttgtgg cgattgcaga tgtctcctat tatgtgcgcg agggcagtat
     1081 tcttgatcaa aaagcaagag atcgcggcaa ctctgtgtat tttcctgatt atgtcttgcc
     1141 catgctgcca gagtgcctga gtaatgatct ctgttcgcta cgtcctcacg tcgacagagc
     1201 ttgtcttgca gccgatatga tcatcacaaa aaacggtaag cttaaacatg tgcatatcaa
     1261 acgtgcatta atgtgttcta aagcacgact cacctatgat gatgtagaaa aagcgattca
     1321 cggcaaggtg aatcaaaagg ttgagcccgt ttatcatagt acgattttac ctcttttaaa
     1381 tgcgtataaa attttgcgcc aagcgcgtga tgaacgaggc agcttagata tccataccaa
     1441 ggaacatgag attttttttg ataaagataa tcatgtgcaa aaagtagagg tgcgcaaaaa
     1501 tctcatcagt tatcagttaa ttgaagagat gatggtcctt gcaaacgtag ccgttgcaaa
     1561 aaccttgggc aaccgtgatt atccgtgcat gtaccggatt cacccagctc cagacactat
     1621 caaggtccaa aatcttaaaa ttttttcaaa agcacttggg cttcctgctc ttaaatctga
     1681 cacgcctaca ccccatgatt ttaatagact tttagaagcc gtgaagggcg gaccctacga
     1741 tggactcatt aatgaccttg tgctgcgctg tcaagcacaa gctcaatata gcccgatgaa
     1801 tcaagggcac tatggccttg gcctaacgga ttatgcgcat tttacctcgc ctattcgccg
     1861 gtatgcagat ttaattgttc atcgtttgct tgtgcatctt tttgaccttg gggagggagg
     1921 cactaagtct accgagggac ttcaaagtat tgctgatcat atttctcaaa cagaacgcca
     1981 agcagccagt gcagaaaggg aagccgatga aagatttctc gcagcgtatc ttgaaaaatg
     2041 tgttggagat acatttgatg ctgtgattgt gggcgtgaat caatctggtt tatttattga
     2101 acttagtgat cttcatgctg aggcctttgt gcctaaacgt gttcttttta atcttacgca
     2161 acgtgtgtct tcgtattttg atcaagcgct tcatattttg cgcgttggtc atttaagctt
     2221 tcaattgggg atgtctttga aagtgcgcct tttagaggct aatgcgataa catgtcaggt
     2281 tatcgccgag gttgttttgg atcaacaagc gactaagaaa aaggacaaaa atccttcgca
     2341 gaacagcaaa aaatctttac aaaaagataa gaaaaaacgc aaattcttat aaaacatagg
     2401 atggtgatgt tcgcaaaaaa atcgcttaag atccttagcc ttttttgggt tgattggcgg
     2461 ctgaaacttc ctttgaagcc tccccctttg agggcgcttt ggatggagga ggcagtggtg
     2521 tttgacgcaa gagcataatg ctaatacggc gattttctgg cgcaaacgga ttttctttaa
     2581 taaaaggctc cctatctgct cttcctacaa cggaaacaat acggttacta tcaaccccat
     2641 tatccacaag aacaccccga ctggcattgg cacgatcaga ggaaagctcc cagttagtat
     2701 aattttttgc attataaggc gtagcatcgg tgtgtcccga aatagaaatt ttattgggta
     2761 ctttttgaat aatttcagcc acacgtatga gaagcctttt cgtgtagtcg tacatttgac
     2821 tgctgcctac aggaaacatc gattttttat gttggtcaat gatttgaatg cgcagcccct
     2881 ctggagtttc ctcaatgaga aggtttttta accattcttt aagacttgga tcatttttaa
     2941 gagctgactc tatgtctttt ttaattgact taaaactcgc ttcctccgcg gcgattttat
     3001 ccgccgtatg tgctgctgcc tcctcatccg tttcttctct ggactgagca cgtgtttctt
     3061 gttctccacc gacacctttt tcctggggtt ttgactcttt aatcgaaagt gtgctcatcg
     3121 aagactgagc agattgctct ttacttgtga tcgagtcgcc catcataaca ccttcaccgc
     3181 cacctgcacg ttcaccaatc gtcacgggat caaagtaatt ggcaatgcct cgcttttgct
     3241 cctcatccgt tgcatttaaa agccacatta aaagaaaaaa tgccatcatt gccgtgacaa
     3301 agtcagcata agcaattttc cacgccccac catgatgccc accatgggca ccttttttga
     3361 tttttttaat aataataggg cgctcatcag acatgtgatt ttatcctgaa ctgaggttat
     3421 taatagcctc ttccaattca taaaacgtag gacgattttg aggatcaatg gattttctgg
     3481 caaactcaat tgctacagct ggcgcataac cattcatata ggcaagaatc cctgctttca
     3541 tacactcata atatttgact tcttcatcgt aaatagattt aagactcgat gccataggcc
     3601 ctacaaaacc ataggcaatc aaaacaccag agaaagttcc aacaagtgcg cccccaatat
     3661 agtgacctaa gacctctgga ggtgaattaa tatgccccat cgtcttgatc acacctaaaa
     3721 cagccgccac aataccaaga gcaggcgtac catccgccat cgtctgaata gctgtaacaa
     3781 cttgctctcg ctcgtgatga tgcacaccga tttcttcatt cattaaatct tccatttgat
     3841 gggggttttc agcacccatc gttatcatgc gcatataatc acaaaagaaa acaactgcct
     3901 cgtgacgtgc taaaaattta gggaattttt caaaaagctc actctccgta gggttttcaa
     3961 catgcgcttc gagggccagc attcccttgc tttttgcaag tttaaaggtt tgaaaaagca
     4021 tcgataaaag atcaacaaaa tcctcttttc caacaagact tcccttcata atgtgcccca
     4081 tggctccccc aagatgagac agaacatgtt tggatgtacc aatcacaagg gctccaatcg
     4141 cagcaccaaa aataatgaca aactcgaaag gttgccaaag gacaggaaca cttcctccca
     4201 tggccgcata gccgccaaga acgcatccta aaacaatacc taaaccaata aggaaaaaca
     4261 taggtaaaag ggacttccaa catctgaaca atgagcctca tcacctaata tcccacatat
     4321 ttattaatga tttataaaga caacaaaagg ttttttttat atttgtcatt ttttcatctt
     4381 ggtctttctc ttttttcacc tctttttgtg tgtttttcat ggatattgtc ctaattccct
     4441 ttaagattgt cattcttacc atatttttcc catcattgag gcgattttcg ggataatttt
     4501 gctattttga tcaacaattt cactattttg aagctttttt cgcccatttt tctgtatttt
     4561 tcatcgaaat tgtgctaatt ccccttaaaa acggtcattc tccccctaat tt
//
LOCUS       DAIAHG010000086         4575 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_86_length_4575_cov_3.477101, whole genome shotgun sequence.
ACCESSION   DAIAHG010000086 DAIAHG010000000
VERSION     DAIAHG010000086.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86_length_4575_cov_3.477101"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1632 a          754 c          925 g         1264 t
ORIGIN      
        1 tgcgttaaaa aagctatttt ttgcccgaaa aacacacaaa atagcgaaaa aaataggatt
       61 ttcgaaggaa aatctagggg cgcactgaga agagaaattt ttaaaaaaaa tcttcgcaaa
      121 aatacaactt accccctctt tttcatcgat ttttgtgaaa aattcaaaaa ataaacataa
      181 aaatcatgaa aaacacagaa aatcttgtcc agaaagcact ctttccatcg aaaattcacc
      241 tcaaaaatcc taaaaaatcg cctagtcacg caaaaaatac actatttttc atgaaatccc
      301 ctcgaaattt cccaaaatgg gctgaaaaaa gggctatttt caaagaaaaa ggcggctatc
      361 cccactcaaa tcagagagtg aaggcctcaa atcatgcttt taatggacaa taatgtgcaa
      421 aatcaggaga taaaagcttc aatttactat ttttctcgtc aaaaatgacg tcatatccag
      481 gaaaattcgc cttaaaatcc taaaaattca ccaaatcacg ggaaaataac cccatttttg
      541 cccgaaaaac acacaaaata gcgaaaaaaa taggattttc gaaagaaaat caggtgtatt
      601 ttcttgagaa tttcaaaaat gaggacgaga aaatcctctc tttgacaaga atgtttatga
      661 gactaccccg aaaaaattgg aaaaaaatct cttcaaagct caaaataacg ataataaaac
      721 ttatcatgtt ccttagcctt tggagaattt ctaaaatgca cttgtatcga tgttaataag
      781 tttggaaaaa cattaaaaag cttgttaagt gcgctttttg gagactctgc ccccaactca
      841 ataagattca aaggaggaat ggaggttatc tagtgttttg tatgaaaatt tgttaggcta
      901 tctccatctt attttaactt catttgatca aaagtgtttg aacgatttat tgcttttcgc
      961 tacctccgct cttctaaaca ggaggggttt gtctctgtta ttgcaggttt ttctttgctt
     1021 gggattgcga ttggtgttgc gacactgatt attgttttat cggttatgaa tggctttaga
     1081 gaagagcttt taggccgtat tattggtgtg aacggtcata tttgggtgct gcctaatacg
     1141 cctacttttg aaaatatcga taaagtttta aaagatgttc gctcaatccc cggtgtgctg
     1201 gaggcgcatc ctaccataga acgtcaggcc gtgttgatgc ataaagggca agcgcggggg
     1261 cttcaagttc atggtatgcg tgtagaggat attaaagagc gatcgattat tgcgaatacg
     1321 ctgaaatttg gcacattaga taatttaacc gagggcaagg ttttgattgg aaagcgttta
     1381 gcggatattt tacaaattaa tgttctcgat accattacac tgttaaatcc cgatggcaat
     1441 catacagcgt ttgggactgt gcccaagcaa aagaattttg aggtggctgc gatctatgaa
     1501 ataggcatga atgagtatga taagaatgtg attttcatgc cccttgcctc tgcgcaaagt
     1561 ttttttaaaa tgaaggggtc gatttcaaat atcgaaattt ttattgatca acctgatcat
     1621 gcaagtgcgt atacagcgtt gattcaatct aaaattggtg atcactataa aacgcttaat
     1681 tggcaacaca gccagtcacc tttttttcag gccgtggaga tcgagcgcga tgtgatgttt
     1741 gtgatcttga cgctgattat cctgattgcc gcgtttaata ttatctccag ccttattatg
     1801 ctggtgaagg ataaaaataa agatattgcg atcctccgta cgatgggggc cacgcgtggt
     1861 tcgatcctta aaactttttt tatcacaggt gccagtattg gtgtggtggg gacgttgttg
     1921 ggggtgattc ttggtgtgag tttcgcaacg cacattgagg aaatccgcca atttcttcaa
     1981 aatgtatgcg gggttgatct ttttaatgca gagatttatc acctcacaaa attccctgca
     2041 aagatggatc tgcgggaagt gttgatagtt gttgggatga gtttatttct ctcctttgcg
     2101 gcaacttttt accctgcgtg gaaggcagcg cgtcttaacc ctgtagaagc gctacggcaa
     2161 taaaaatata atattcgcaa taaaataaac cttttgttaa gttttttgtg atagatattg
     2221 agtgataaaa acttattaaa ggaatattac catgaataaa acatgtaaaa taattgcgac
     2281 atccatggca tttatgttgg ggggggcgta tcataactct cctgcaatgg ctgtgaagat
     2341 taaaaaggaa gaaaaaaatc tattcgtaaa acagattcga cagaaaagag aggaagaaaa
     2401 aaatgcccaa aaacaattgg aaaagacacc tctcatagat tatgaaaaat ttaaaaggat
     2461 tcttaatgca ttaaaggtgc tgggaaactc ttcagagaat cgcgaagatt atttttacct
     2521 tcgtaatgaa acaaagatgg agatagaaaa attccttttg gacaaaattt acactctcta
     2581 taatgatcaa aaaccagagg gtaatttcga gaatattttt ctagaaacag tcaatactct
     2641 caatgataag atgcgttccg tttaccagca atttagtttt gacgaaggtg aaaaaaagga
     2701 agaaaacaat ccaaaaccct tgagtcttaa tgataaggaa ctccaagagg taattgaatt
     2761 ctctagaaaa atgaacgatt atcagggtat atggatagac cctattgttg ctaaggcctt
     2821 tgcacaacta aaaaagcctg aataatataa gtgggcaggg cagtggtact gcccactccc
     2881 cccccttcaa tcctgtagaa gcgctgcggc aataatcatt aacactcaga ctgaagatca
     2941 atatgacaga tggattggga aaagaaaact accatgaatt tggcgcagaa gcagatcgcc
     3001 gaaaccaaag gagacgagaa gaagaaaaga aaaaaagaaa aaaagaaaga agcagacaga
     3061 aaatttgacg aagaacgaaa acaaaaagaa caagaggata cgcatagaat cgttggtaaa
     3121 ataatcgatg tgcggggtaa attatggaga tcatcaaggc tctagggcac tgacgcctcc
     3181 ctcactaacc ttagattact atgagtcaac cggaaaagac taagtttatg aaaaattaac
     3241 gatcttaatt attgtgagga agggctgcaa ctaaattatt gtttattttt tttattaact
     3301 ttttgtgcta tgaacttatt gatggatgaa taacacaaaa taaaaggaat ataatgaaca
     3361 aattaaatac aatgattgca tcttctatag cgcttgtgct gttgggggcg tataacgtag
     3421 ctgatgctat ggataaagat gaaaaagaaa tcacgaaaaa agacgtttac tatgtttcac
     3481 aagaggaaca aaaaaagtcg aaaaaaaatt aaaaaatgtg ctaagtcgtt tgtattcctg
     3541 tgcaggtttt aattttaaag aattttctaa tcaactacgg aaggaaagag aaaggaaaag
     3601 agtgacagaa aactcttttc tggaagctgt taacgatgtg gatagagctc atgattgtat
     3661 aaatagtctc tgtaaagaac ttggaataga ggaaaagaca tcagatgaat ggatcgataa
     3721 taaaatcaat agtttctcgg atattcttaa tctgacagca gatacatttg aaaaagcgcg
     3781 cacacagggc attctcacaa aaaatacttt tgaagatgtt atgaaattat gtgaaacaaa
     3841 gagcaaaaaa ttctctccac ctacaaaaga ccgaatgctt catgaaaaca aggaaattct
     3901 tgatacattt attaaagacc ttgagaaaaa attaaaaaaa gatacttacg ataaaaatcc
     3961 tatgaatgac ctcattaagg aaaggcatgc tctgttcatg cttgagaaag ataaaaacca
     4021 tacagagaaa agaattagac aatgtgtcca aacgataaaa agtggtgaga ataggctgat
     4081 agatttcaat gaaaaattaa aaaaagatcc aaacaataaa agtgctaagg acggtattcg
     4141 tgaaatagat ggcatcgtca aaacaaataa agacagaaga gatttcttca taaaaattat
     4201 tgaaaggatt gacgcagaga taagcagaaa aaatgaagtc attgaaagcc tcgagaataa
     4261 atttaaagaa gatactgaag aataacgctc aagaccaacg acgggttgcg cttttataca
     4321 atttgtgtac aatggcgtag ccccttcttt tgcttaaata tatgacgcaa cagccctttt
     4381 gtctgcagct ttcaaacatc cgtcacgatt ttaagcaagg tgatgaaacg attcatgtgc
     4441 tcaaaaatat taatcttggg attaagccgg gcgagcttgt cgctcttgtg gggcccagtg
     4501 gttcagggaa aagcacgctg ctacagattg cggggttgct tgaaaaaccc tccgccggga
     4561 ctgtatctat ggatg
//
LOCUS       DAIAHG010000087         4542 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_87_length_4542_cov_2.941993, whole genome shotgun sequence.
ACCESSION   DAIAHG010000087 DAIAHG010000000
VERSION     DAIAHG010000087.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87_length_4542_cov_2.941993"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1282 a          913 c          962 g         1385 t
ORIGIN      
        1 tccccgccac cacatggggt gtgataaaat ctaaaacttc tgcggccaca cgtcctaccg
       61 cacgcatccc ctcgaaatca acagctttgt gaagtgtaat cacgtcttct tcctcgtgat
      121 gatgatcatg agagcatccc tcatggtgat gatgaaccat ttctttgagc attatagatc
      181 cttatttctt tctcttttct taagatttag catttttaaa gggggataga caatttctaa
      241 gagacgtcta tttttcaaaa aatcgtcttt agaatagcat agcaatggaa cggaatacag
      301 cccattaaag tgtattcaaa gcaaggaaag ctggatcaat ccactttttc acacgaatga
      361 tttttcctca tattaatgag cttagaacct cgcaaatgtc gcattaacaa tagacattaa
      421 agtaaaatca tctcctaaaa aattgaaaac tctacgcttc aagggccaaa cgctttatac
      481 tcatcccatt atcaggacac taaaatattt tatgcttgcc ccctcacttt tttttctgtg
      541 tgccaatatc gcggatattc tttttgaaaa aacccaagga acgcttactt tgcttgtgtt
      601 tcatgttctt tttttcaaat cccccacacc tccatcgttc tttttatgcc tgttaagtgg
      661 gttttttatc gatgcttata gccatcatgg ctttttgggg gtgacgagtt tgtacctaat
      721 tctttgcttt atgttccttg aacggattaa aatttgggtt tataaactag gtaatttttg
      781 gctaatatgg ggaagttttt gtgcaattgt tttaatgtat aaagtcacgt ttatgacctt
      841 tttttacaca caaacaggta cattccatta cgcacgttca tcctttttat ggcttttttt
      901 tatgatctcc gtttaccccc tcgtttttca ttttttcccg gtgattaaac gccgtgtttg
      961 accccaaaac aacctttaca aggcgcgctt tttttttagg aggtatgcaa ggaattttag
     1021 gggctggact ccttggacgg ctttattggc ttcaagtaga atcaaacgac cactataaaa
     1081 tgctctccga taaaaataga ctgcacaatg aatttctgct gcctattcgc ggtcaaattt
     1141 ttgatacaca tggacagctt cttgcaggaa atcaatttat tcaccgtgct tttttacagc
     1201 gtgatggtac tgaggggtgg caaaaatccc tcgaccgatt tgcagagctt ttaaatcttt
     1261 ccacaggaga ggtagaatct cctctcaaaa cagcaaaata taaaaataaa tttcttccct
     1321 tgtgcatcaa agatcctctg acatgggagg aggtcgccac gattgagctt cataatcaag
     1381 aacttcctgg cattcatgtg gagcaaggac aatgccgctt ttatcctcac gctaagattt
     1441 tttgtcatat tctcggctat gtcggtcagc caagtatcaa ggataaagaa gaacttctaa
     1501 gtcatcccgt tgcacaacat ccaggctttc gtatgggcaa agcaggtgtt gaaaaaatgt
     1561 ttgagaaaat tttacaaggc actcccggga ttaaacaagt cgaggtcaat gcaacccgtc
     1621 agattgtacg caatctatcc atatcccccc cccataatgg tgaagatctc acgctctctc
     1681 tcaagctgga tcttcaaaaa agcatttatg ataggcttag tctttttgaa agcgcaagtg
     1741 ctgttgtgat ggatgtccgc tctggtgggc ttttggcgtg tgtatcaaca ccatcttttg
     1801 atacgaatca ttttatgaat ggcatcccac gccacttatg ggaagatctt aggtcaaatc
     1861 cgcattcccc ccttgttgca aaatttgccc aaggccaata tgcaccaggt tctgttttta
     1921 aaatgattgt cgccttagct ggattaaaaa gtggcgtcgt cgatgagcac acaacttttt
     1981 tctgtccggg ttattatgat cttgttggga aaggcggaca catgcaccgc tttcattgtc
     2041 acttaaaagg cggacatggc cacgtgaatc ttcagcaagc cttggcaaaa tcctgtgatg
     2101 tttattttta tcatttatct cttctttgcg gtgtcgaagc cattgcacag atgtctgcac
     2161 tctttgggct tgggacgcca acaacacttg gttttcctgg agaaaaagcg ggtctcattc
     2221 ccagtaaaaa ttggaaagag caactgcgtg ggaaaaaatg gactcccgca gagacgatta
     2281 atacaagcat tggccaggga tatgttctga ccacacctct gcaattatgt gtgatgacag
     2341 cacgtcttgc ctcaaaaggt cgtgcggttc agccattttt aatacaaaat cagcgggttt
     2401 ttgagtctct tgatattgac atcaaacata tggatcttgt cttccaagga atgcgcgacg
     2461 tcatgagtga tgtttccggc acggcttatg catcgcgctc gcctttgcct cacattgaat
     2521 ttgctgggaa aacaggcacg gctcaggtgc gtcgcattac aaagcaagat agagagctgg
     2581 gccttcataa aaaatggcca tggcactggc gcgatcatgc cctttttgtg ggatttgccc
     2641 ctgtgcatga tcctgagtac gcggtttctg tagtgatcga gcatggggga agtggcggaa
     2701 aagttgcagc cccgctcggc cgtgacattt tagtggacac tttcaaatat ataagcgctt
     2761 agatttattt aatattagct ttgaatacac ctttttaaaa atttaattcg atgtcgacac
     2821 attttcggaa atttcgaggg tttttcatga aaaatagtgt attttttgtg tggttaggcg
     2881 aatttttggc cgaattttcg aaaaaatagt tgtgctggag tggtctgtat tgagtgattc
     2941 tcgtgtcttt gagatcggca aaaaggctgt aagcgttttt tagaaaaatt ttcccttctt
     3001 aacagtcgta tgaaactagg gtttttcatg aaaaaaatgg cttttttggg gagaaaaata
     3061 ataaattcaa gcttttatct cctgattttg cgcattattg tccattaaaa gcatgatttg
     3121 aggcctttac tctctgattt gagtggggat agccgccttt ttagggaaat cagcgcacta
     3181 tccatgaaaa acacataaaa agaggtgaaa aaagcttcaa aatggtgcca ttgttgctca
     3241 aaaataaaaa aaagcttttc tgagcttttt aaaaaaggta aaaaatcgct ttttttcatg
     3301 ggatccagcg tgttttccca aataatgtgc ttttttccaa aactttcgtg ccatcgtcat
     3361 taaaataatc tctaaaaaac gttctccctc ttcctaagca gacttgaata ttcttcaaaa
     3421 taccgggaaa aaacaaaaat acacagcaga agaaatcata ccccccaaac aatagatcta
     3481 aggcgtgagg gcctctcctt tgatcacacg cgttacagta aaggcataaa tatgacgcac
     3541 cgaggctttg ctaagagctt ttgcacaggc tttgagcgtg gcgcccgttg tatacacatc
     3601 atcaatgagc gcaaccgatt ttccctttaa aatctctgga taatgcacat gaaaagcacc
     3661 acgcatgtta cgtgtgcgat ggagatagct tttaaggcct tggggttcgg tgtattttgt
     3721 tcgcaaaaga agattggtca gcacaggtat aggaacatca cgccttgcag caatgctatg
     3781 tgagagatga tggcaaagct ctgtggcttg gttataacga cgactccaaa gacgtcgcca
     3841 atgaaggggg acgggaagaa tataatcaat atcctcaaga aagggctcaa ttacaggata
     3901 gagaaacgat gcaagaggct tagcaagatc catacgatca aagtatttaa gtttgagcac
     3961 caattctttt gagaggtcat tataacaaag gggggtgcgc agcgtataaa gaggcgattt
     4021 tgcaagacac gggccacagc gctgcccttc ttgggcattt tctaaaggat agccacagca
     4081 ctcacataca tggccttgga caaaaacgag ttggttaaaa caatcactgc aaagggttcc
     4141 tgcatcgctg ataaaacaag agcatgccag gcattctggc ggataaaaaa aatcaaaaac
     4201 tgatttgagt cctgacatgg ggtatgacga aaataaagaa ttctcccttt ctatgataga
     4261 gcgtaaagta ttaataaatg aataaagtaa cttatgtgta tagtgctttt tggcgggatt
     4321 aattttatgt atttgccgta gaggtagtga aggttttttt aaaaggccct ttgccggggg
     4381 ttttctagtg gtgtcaaaat gctttatggg gggatgcaga tgggcatttt tcccattttt
     4441 atgcattttt cccattaaaa agcgattttt gacggcatcg tgtgaaaaaa tgcctcgatt
     4501 tgagcgggtc gcttggtgaa tttttttggg gcgtgctaag ac
//
LOCUS       DAIAHG010000088         4513 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_88_length_4513_cov_3.307710, whole genome shotgun sequence.
ACCESSION   DAIAHG010000088 DAIAHG010000000
VERSION     DAIAHG010000088.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88_length_4513_cov_3.307710"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1363 a          831 c         1101 g         1218 t
ORIGIN      
        1 caccaaaatt acctcaaaaa aggcgtactt ctcatgcgaa acaactcaaa tatgcactac
       61 tgcgatcaaa aaaggcctat aaaagcgaaa aattcgggga aaaacaggaa aaaatcttaa
      121 aaagaaggaa gatgatcttg atttcccttg gcaaattgaa tttttgatag ggtacaaaaa
      181 ttactaaaat tctactgagt gcgtgcttca aaaggtgatt cataagggaa taatagagaa
      241 atcgcaggca tactatttct acaggcatac tatttctagg aggattggtt tttcatagga
      301 atcaatgctc tgtgagtaga gctctccact tcttttcaga aaatgcatta atcgatgcag
      361 aaaggacttg taaaaaaaat gaaaagccct tataaccgta atacttaagc aggaagtgcg
      421 ggatgggcgg cgaaagctca aaacactttt tttgttcaaa aatattaaac atcaagaaat
      481 gttaacttga tcttttgagt gagtatatta tggagaatca aaacatacgt attcgtctga
      541 aagcgtttga tcataggctc ttagaccaat cggtacgtga aattgtcaat acggctaagc
      601 gcacaggtgc gcaggtgagg gggcctattc ctttgccaac aaaaattgaa cgctttaccg
      661 ttctccgctc acctcatgtg gataaaaaat cccgtgagca attcgaaatc agaacccata
      721 aaagacttat cgacattgtt gattggttgc cacaaacggt tgatgctttg atgaagcttg
      781 acctggcagc aggtgttgat gtcgagatta aactctagga gctcacacaa tgcgttcagg
      841 acttattgca aaaaaactcg ggatgatgcg ctactttcaa gaaaatggcg aacaagttcc
      901 cgtcactgtt ttagagatcg acaattgtgt tgttctcgct cataaaacag aagaaaaaga
      961 tggttacgtt gcccttcaag taggggcaga gattgcgaaa caaaatcgcg tatccaagcc
     1021 agaaaagggc tatttctcaa aaattaatca agaaccgcgc aagcatgtgc gtgagtttca
     1081 tgtctcagcc gatcacctga tcgatgcagg cacagcgctc acagcctctc attttgaaca
     1141 agggcagctt gtggatgtga caggtacaag cattggtaaa ggctttgcgg ggggtatgaa
     1201 acgttggggc tttggtggtc ttcgtgcaac gcacggtgtc tctgtttccc accgtagtca
     1261 tggttcaaca ggtaaccgtc aagatccagg aaaagtcttt aaaaataaaa aaatggctgg
     1321 tcatatgggg gcaaggcgtg ttacaaaatt aaatctcgtt gttcacagtg tggatgccga
     1381 tcgtaatttg atctttatca aaggatctgt gccagggccg aagggtgaaa tcgtctttgt
     1441 ccgtgatgct attaaagcgt caaagaaata agtagagaag tgcgatgaaa gtagacatta
     1501 ttaattggaa taaaaaaaag gtgggtgagc tcgatcttaa tccatctgtg tttgcagcac
     1561 cgctgcgcag cgatattctt tcacgtgtga ttcactggca gttggcaaaa cgccgtgctg
     1621 ggacgcatgc tgttaaagag cgtggagatg tagaggggtc aacccgtaaa atttataaac
     1681 aaaaaggaac cggtggtgcc cgtcacggtg cacgcaaggg tgcgcagttc cgcggtggtg
     1741 gtatcatttt tggtcccgtt gtgcgtgatc acgggtattc gttgcctaaa aaagtgcgca
     1801 agcttggtct caaagtagcg ctctcttcaa agcttgcatc gaatcagttg tatgtcattg
     1861 atagtcttga catggctcaa ccaaaaacag ctgagcttgt aaaggcgttt ggtcaatttg
     1921 atacaccttc tgcgcttttt atcgatacga agaatgtgaa tgatagcttt ttgaaagcaa
     1981 gttcaaacat tcatacgatt gactttttgt ttgatgttgg tgcgaatgtg tatgatatcg
     2041 ttcgtaaaaa cacactgttt gtaactaaag atgctattgc agcgctagag gctcgactcg
     2101 catgaaaaat ataaatgcat ttaacatatt aaagatgccg atcatcacag aaaaggcaac
     2161 gacggttact gaatttggtc agtattgttt tgaggtcgat agagactctt caaaaactca
     2221 gatcaaaaag gctgtggagc atattttcgg tgttaaggtt aaggctgtga atacgcttat
     2281 ccttaaggga aagaaaaagg tttttagagg tcacattggt tttcgtaaag acaaaaagaa
     2341 agcttatgta actcttgatg ctggtcaaaa aattgatata agtgcgggtg tataacgatg
     2401 gcgctgaaaa catataaacc aattacgaac gcgcaaagac agctcgttaa tattgatcga
     2461 tctgagcttt ggaaaggtcg ccctcttaag ggattgactg ttggtaaaac aaaaacaggt
     2521 ggtcgcaaca atcatgggcg catcacgtca tggaatcgtg gtggcggtca caagcaactc
     2581 tatcgtttga ttgactttaa acgtcgcaag caagatgttg cggcaacagt tgagcgtatt
     2641 gagtatgatc ccaatcgcag tgcttttatc gcacttctta aatatgaaga cggtgtggat
     2701 gcctacattc ttgctcctca aaaagtggct gtaggcgata agattattgc gggcgatcgc
     2761 gttgatatta agccaggaaa tgctttgccc cttaaaaata ttcctgtggg caccattgtt
     2821 cacaatattg aaacaaaagt aggtaagggt ggtcagctcg cacgttctgc tggtgcttat
     2881 gcacagatcg caggtcgtga tgctgcgtat acaattataa aattatcttc gggtgagctt
     2941 cgtctgatta agggcgagtg tatggcaaca gtgggtgctg tctctaacca agatcatcaa
     3001 aacagatcct atagtaaggc cggtcgtagc cgttggcttg gacgtcgtcc aatcgttcgc
     3061 ggtgtggcaa tgaaccctgt tgatcaccca catggtggtg gtgaaggaaa aacatccggt
     3121 ggacgtcatc ctgtgacgcc atggggtgtt cctacaaaag gtaagaaaac aagaaccaat
     3181 aagcgaacct caaagttcat tattagaaga gcaaaataac ggagtgaatc gtgacaagat
     3241 cagtgtggaa aggcccattt tttgatcgct atctccttgg caaggctgag gcagttaaag
     3301 cgtccgggcg taaagatgtg attaaaacat ggtcaagaag gtcaacgatt cttcctgact
     3361 ttgttggggt gacattcggt gtgtataatg gccaaaagtt tattcctgtg tttgtgacag
     3421 aaaatatgat tggtcacaag tttggtgaat tttcaccaac acgtacctat catggtcact
     3481 caaagacaga taagaaatca aagaggaaat aatgacaatg aatacagctc gcgctgagcg
     3541 caataaaatt cgtgtaagtc ctcgtaaact taatctggtt gcgaagcaga ttcgtgggat
     3601 gacagctgca aaagctttgg atgctttgaa attttctcct aagcggtgtg cttctgaggt
     3661 gtataaagcg gtcgcttctg ccgttgcaaa tgctgaaaat aatcatggac ttgatgtgga
     3721 ctttttagta gtatcgagtg caaccgtagg ccaggggttt acgatgaaac gtatgcaccc
     3781 tcgtgcaaaa ggacgtggtg ttagaattaa aaaatacttt agccatttgt ttatcgaagt
     3841 ggcagagaaa aaggcgtaac gatgggacaa aaagtaaacc cgattggact aagacttgga
     3901 atcaataaaa cgtgggattc acgttggttt tcctctaaat cttatggttc tatgcttcat
     3961 gaagatctta agattcgttc ttatattcat gagaatttaa aacaagctgg tgtatcaaag
     4021 gtcgttattg agcgtcctgc aaaaaaagcg cgtgtcaaca tttatacagc acgccctggt
     4081 gttcttattg gtaagaaggg tgcagatctc gataaactta aagaaaaact atcaaagttt
     4141 acacagtcag agctcaccat taatgtggtt gaggtgcgta agccagagct cgatgctaaa
     4201 attatagcgg atggtattgc tcaacaaatc gagcgccgtg tttcctttcg tcgtgctatg
     4261 aagcgtgcac agcaaacggc agcgcgcctt ggggctcttg gaacacgtgt gaactgttcg
     4321 gggcgtttag ccggtgcgga aattgcacgc gtcgagtggg ctattgaagg aagtgttcct
     4381 ttgcacacat tgcgctcgga cattgactac gctttgagtg aagcgcttac aacctatggt
     4441 atcattggta ttaaggtgtg ggtgtataaa ggtcagattc ttgagcatga tccaatggcg
     4501 catgaaaaaa gaa
//
LOCUS       DAIAHG010000089         4511 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_89_length_4511_cov_3.046234, whole genome shotgun sequence.
ACCESSION   DAIAHG010000089 DAIAHG010000000
VERSION     DAIAHG010000089.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89_length_4511_cov_3.046234"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1361 a          850 c         1002 g         1298 t
ORIGIN      
        1 accgttttct tcaaaaggcc aatcccaata gcgggcaagc tcataaataa aaaagggaat
       61 cattttttga ataattggcc aatcctcaag ggttgctttt gtcaacgtga gcattttatt
      121 tttcatcccc cttgaataac aaccgatgaa tccggtgaaa gatacgcgat gatttctctc
      181 aggtccccct cgctaaatgc cacacatcct gctgttcctg tatagccttc gcgggcaata
      241 tgaataaaaa tagcactccc tttaaaaggc acaacaggat cgtcgttata cccaacctcg
      301 ataatcatgt gataaacatc atcatcacgc cagagctttt catggctaat ggtaaggtca
      361 aatgttaaaa gatcaacgcg tttgttatag tcaaaatgct gaggctcatc gcaccaccca
      421 tcactgggtt ttagcgcatg tttggggaga agagtttcaa ggtcactctt tttataaata
      481 tcgtcgcggt aaaaaagtct gcgaaggggg aatgtccctg tgggggtgga accgtcgcct
      541 tcttttttgt ctgtggctgc gataaaacca tttcttccca cagcacaggt gtacgttttt
      601 ccgttgatcg ttgccgtata atgactctct ttttcacgaa catacaggtg ttgtggcttt
      661 tccataaacg cttctctttt cgtggagcaa tgcccttttt ataatatttt ttcggtctta
      721 ggtaaatgtg gttatttttt ttaatttctc tcctacttag ttcgtgggtg ctaaggttta
      781 tgaggtgtat gggaaaaaaa ttctctaaaa acccattttt tttatttttc ggcatatttt
      841 tcctaccaaa gccccatttg ttaacgattt gtttagaatt gatgccctag gatgaagtta
      901 taaattttaa ataaatacac ttaaaagtga acgaatttaa agagttcctt acgaatcttg
      961 gtacaggcaa gctcatggtc ttgatcggag ccgccatggc ctcttttgcc tttttagctt
     1021 accttgcttt tcaaacgaca aaacccgata tggcactgct tttttcacaa atagatgaag
     1081 cggatgcagg acatgttgtg gaacgccttg aatccatggg gataccttat gagttgcgtg
     1141 ggaatggcac acaaatttat gtcccagatg atcgcgtggc gcgtattcgc atggatctcg
     1201 ctcaagcagg tattcctcat tctggttcag ttggctatga gatttttgat aaaatggata
     1261 ttttaggcat ctcgagtgcg atgatggaca ttaatatgtt acgtgcactc gagggggaac
     1321 tcagcaaatc cattcgatcg attcaaggtg tctcctctgc acgagtgcat cttgtgatcc
     1381 ctaaaagaga aatttttagc aaaagccatg ttcaacccac ggcatcgatt gttttaaaaa
     1441 tgaattatgg gcgccttaac caagcacaag tccttggcat tcaacatctt gtcgcatcgg
     1501 ctgttccagg gctttcttat gagaaaatta caattgtgga tgataagggt aatcttcttg
     1561 tcaggcaaag cgatggaagt ggttttgatg tcagtaataa tcaagagata cgttctagtt
     1621 atgagcaaaa attagcccaa acgattgaaa gccttttgga gaaaaccctt ggtccgggga
     1681 aggcacgggt tgaggtgaat gcagatatgg attttgataa agtcacgatt aattccgagg
     1741 actttaatcc taatggtcaa gtcgttcgct ccacaaatac aacgggtgag aatggattga
     1801 gtacagaaca agataatggg ggggggggta tttcagttca aaatgctttg ccaactgcca
     1861 caggcggtag cagtgctgat aatacaaagt catcgaataa aaatagtcgc aatgaggaaa
     1921 gcactgttta tgagatttct aaaacactga aaacacaagt gaaggaatct ggcactgtta
     1981 aaaaactttc tgtggccgtc cttgtggatg ggatttatgg aaaggatggt aaatacagtc
     2041 cgcttcctaa agaagaacta gataaacttt caacacttgt caaaacggct gttggcttta
     2101 ataaagcgcg tggcgatgtc gttgagctta tcaatcttcc tttcgcaccc tcagaagccc
     2161 ctgatgcgcc tttgacaatg gttgataaaa ttctatctca agtgagcttg tcacatattg
     2221 ctgatattgc tatcccaggt attattggac ttttaaccat tcttcttttt gttaaaccgt
     2281 ttatcgggcg tgtgatagag gaaattaagg catcagcaaa ccccattctc cttccttcag
     2341 aaggtgatgt gcaactatca acaaatcaaa atcctatgat gattgagaat aaagccgagg
     2401 aggagatccc accgcctgtg aattcggctc aaaatattcg ggatttgatc gagcaacacc
     2461 cagaagaaac agtgaatatt attcgcaatt ggatggcgac acctactgag ggagccaaac
     2521 gatgaaaatg acctcagatt atgcaacgct taaaggaatt gaaaaagctg cagcgttttt
     2581 gctgacactt tcagaagaac aaattggaaa agtttttgaa cgaatggatg aggaagaaat
     2641 taaagaaatt tctctgacca tgtcccaatt gggtaagatt gatggggcca ttatggaaaa
     2701 gctccatagt gatattaaaa aagaacttta ttcatcaggg gcgattatag ggagttttga
     2761 tacaacagaa aaactcttgc aaaaaattct tccaaaagat cgtgctgaaa gtatcatgga
     2821 ggaaattcgg ggaccagcag gacgcacgat gtgggataag cttgcgaatg ttcacgagtc
     2881 gcttctcgca aattatctta aaaatgaata tccacagaca atctctgtta ttttatcaaa
     2941 aataaaaatg gaacatgccg caaaggtatt gagtgttttt ccagataacc ttgctatgga
     3001 ggttgtcctt cgtatgcttc gtatggaacc tgttcaaaaa gaaattattg aggatatcga
     3061 gcgaacgctg cgcacagaat ttatgagtag tctctcgcgg tcaaataaaa gggatcctca
     3121 tgaaatggtt gcagagattt ttaacttttt cgatcgttca acagaagctc gctttatggg
     3181 tgcacttgaa gaacgcaatc cagaatcctc tgagcgcgtc aaatcattaa tgtttacctt
     3241 tgatgatatt gttcgtatta gtgcccaagg gatccaaaca ctcattcgcg tgtcggataa
     3301 atctaaactt gggcttgctc tcaaaggcgc caacgatacg attaaagatc ttttcttccg
     3361 taatatgtcc gagcgtgctg ctaaaattat gaaagaagat atcgcttcac tcgggcctgt
     3421 aaaaatgaaa gatgttgagg aagcgcaaag cgctattgtt cttacagcga aagatctttc
     3481 ggataaggga gagattgata ttaggtcaag tagtgattct gatgatgaaa tgatagggta
     3541 gaaaaaatga gtcatagtac actttttaat aaaaaatttc tctttgaaac agattttgat
     3601 caaacggctg tcgaaaaaac gtatgaccaa caagatatcg atattgcatg tcgtcaaagt
     3661 tatattgagg gtgttgatgc agggcgcctt caaacactta aggaaattga tgcagaatgc
     3721 cgcacgatcc tgacgcatat tgcaggggca cttcagaata ttcgggcaat tgaacagcaa
     3781 agttttatga atgcagggat tattgctgaa aaaataacaa aagcaatgtt tccgactttt
     3841 tttgaaaaag gtgctttttt agaggttcaa actgttttga gcaacgtctt agaaaagctc
     3901 aatagtacga atgctttaac actttattgc gctcaagaaa tggagcctaa gcttaaacag
     3961 tttattgata cgcttcaaac agacacgttg atcaatattc atgctcaaaa agatatggct
     4021 atttcggata ttaaaattga ttggaaaaca ggttttgcgg agcgtgatca agggctactt
     4081 caagaaacaa tcgataatct tctagaaaac tatacaacag agattcaaac aaagactcat
     4141 gagggagaac aataatgtta tgggatgacg aaaacacaga aactgtgggg gacgcgggta
     4201 ttatgcaaga tgatattgat gctcttatgg cgggtgagag ctccatggaa caacgtgcag
     4261 ggagcccccc ccccccccct tcgcatgaaa tgaatagcgg tcttccaaaa cacgagcttg
     4321 ttccctctca acaggctaaa ggtcaggagg ggtatgatct caatgcggtt tatgatattc
     4381 ctgtgcaact ttcagctgtt cttggaaagg cgagtatgcc tgtgaatcag cttctcaaac
     4441 tggggcgtgg tgctgtggtt gaactggatc ggcgcgtcgg tgaggcgatc gatatttatg
     4501 tcaataataa a
//
LOCUS       DAIAHG010000090         4510 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_90_length_4510_cov_2.993684, whole genome shotgun sequence.
ACCESSION   DAIAHG010000090 DAIAHG010000000
VERSION     DAIAHG010000090.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90_length_4510_cov_2.993684"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1279 a          995 c          940 g         1296 t
ORIGIN      
        1 tgtatcaaat ttatgctcaa tcgcctcatc aagagactca gctgatatta ctgttttcgt
       61 atcggttgca agggcatgtg caatatcttt cccgctatga ttcccctgcc ctacatccaa
      121 aataagatca tccacactgg aaaaatggaa aatttcctgg gctcttttga ggttcccttc
      181 ggtgtaatca atattttcct tggcaaaggc cttgtgcaac aacgatttac ccaactcaaa
      241 aaattgattt ttttgctgtt ggcgggtaaa ccttctaatc cgcgcgcgcg cttttcctgt
      301 gaccacaaaa cgttcccacg tgggcgaagg actttgggtt tttgacgtaa taatttcgat
      361 ctgatcccca ttatgaagca cagtacgcaa gggaacttgg cgaccattaa tgcgcgctgc
      421 aacagtatga tccccgatat cagagtgcaa ggcgtaggca aagtcaatac acgttgcgcc
      481 acgcggcaat gggatcacat cacctttggg tgtgaaacaa aaaacttggt ccgaaaacat
      541 ctcaagtttt gtatgctcga gaaattcttc aggattggcc gtttgttcta aaatttccaa
      601 aaggcctcgc agccacgcat attgcctgcc atcatgacgt gacccactcc cctgcttata
      661 ttgccagtgc gctgcaacac caagttccgc cacagaatgc atatcttttg tgcgaatttg
      721 aatttccacg cgctgattaa ggggcccgat cacggatgta tggagtgatt tgtaaccatt
      781 tgtcttaggc gtagagatat agtccttaaa gcgccccggc atgagagaat attcaccatg
      841 cagcaccccc agaacctgat agcaatcggg tatttcttca acgacaatgc gaaacgccat
      901 aatgtcagaa agctgctcaa aagccacatt tttcatatgc attttgcgcc aaatggaaaa
      961 aggcgttttt tcacgccccg tgattgatgc tttgacatta tttttatcaa ggactttgcg
     1021 catttcgaac aaaatcgtct cgacctgatt ctcgctcgtt tcgtgtaaat agcgcaaacg
     1081 gctgaggaca gactcacgcg cttctggatt gagcattgca aaggataagt cttcgagttc
     1141 atctttgaca ccctgcatgc caatacgctc tgccaagggt gcgtaaattt ccatggtttc
     1201 cctcgcaatg cggcgttgct ttgcgttatc cttcacatag tgaagtgtcc gcatattatg
     1261 cagacgatcc gcaagtttca caatcaatac gcgaatatcc gaggacattg ctaagacaag
     1321 ttttctaaag ttttcggcct gcttcgtttc ctcggattgc aactcaatgc gcgatagttt
     1381 tgtaacgcca tccacaagat gcataatttc atcgccaaaa agcttttgaa tatcctccag
     1441 cgttgcaagt gtgtcctcaa cggtatcatg caagagtgca gtaataatag atgctggatc
     1501 catgcgcata tcggtcaaga tacccgcaac ctcaagggga tgggaaaaat aaggatcacc
     1561 cgatgcgcgc acctgtgtgc catgggcttt catagcaaaa acatacgctt tattaatcga
     1621 ctcttcgtct acctcaggca tatagctttt gagcttttgg atgagctcaa actgatgaat
     1681 catagatcac ataatcgtcg aaagagggcg tcctttattg tgccaaaatt atcggttaaa
     1741 agaaagcctc tctcatctcc tgcgttaatt taaaggctcc tctttacact ttgtatatcc
     1801 gaaaagaata tcttgatacg tcttatgtga taatttttga tgtatcaaac aatttttcaa
     1861 aattttttgc tccatcctga ccttcttatc aaaatcgtga ttatactctt tgtaaagctc
     1921 tttaattttc tcaacatatt ctttattata ttcatgaaaa ttgaaactat ttttattatt
     1981 tttctcttcc ttattaatgg tttctttgtg gtttttaatt ttttccaaaa gattttgata
     2041 tttttcatcg ttcactaaag cagtttcaac ggtttttttg atttttagaa atatttcaaa
     2101 atcatgttct ttgagatttt ttaggtcgtg ataatttttt cgcatctcat tcaaaatttt
     2161 cttttcttca ttatagttta cagtatcatt ttctttattc tcaccgttat tactcgccca
     2221 cagattgatg aagctactaa aaaacacgac aaaaaaccat cgatgcataa aaaattctcc
     2281 ttatcaaagt taaatagtaa aaaatgtttg tgtttttgaa aaaagcgtta gaaaattctc
     2341 cgtcgttgcc tttgcaaggg tcggaagtga cacaccttta atttctgcaa tgcgctctgc
     2401 tgtgtgcacg acaaaggcgg gctcgttacg ctttccccga aaaggcacag gcgcaagata
     2461 cggcgcatcc gtctccacaa gcaagcgatc aaggggcaca aaagcggctg cctcgtgaac
     2521 attttctgct tttttaaacg tgacaatccc tgaaatagaa atataaaaac cacggtccag
     2581 tgctgcgcgg cacagatcca tcgaactact aaaacaatga aaaacaccgc caacgtcttt
     2641 gaactgatca aggactgcga gcgtgtctgc gtccgcatct cttgtatgga tcacaagggg
     2701 aagattgagt gtgcgcgctg cattacaatg aatttcaaag gatttgattt gcgcttcctt
     2761 tggactttga tgatagtaat aatcaaggcc tgtctcgcca agtcccacaa cctttggatg
     2821 gttcgctaaa tccgtgatct ctttgagaag tttttcggca tcatactcct gtgcatcatg
     2881 gggatgcaca cctacagtac aggcaacatt cgcataacga tccgcaattt cctgaagggt
     2941 ttttgcctcg ctcaagcggg tattaatcgt taaaaaaccc tgcacaccct gatcacgggc
     3001 gcgcgtcaaa accgcatcaa gatcttcctg aaaatcagga aaattaagat gacaatggct
     3061 gtcaatgaac atcgtatttt tttaattaaa aatttcctta atcaccatac atcatttttt
     3121 atttttgaaa agggggaaag ggctaaaaac actaaaactc aggccctatc ttttgatatt
     3181 ccaggccttc atatcgctta agacttgcat ccacagaggt ttcgcgctac caaagaaaga
     3241 agaccctttc attctttaat agacctatga cagaaaaaaa taaaatttcg ctttcccctg
     3301 aacagatgat caaagcttat caagacatgt tgctgattcg tcgctttgaa gaaaaagccg
     3361 gacaacttta cggcatggga ctcatcggcg gcttttgcca tctttatatt ggccaagaag
     3421 cagtcgtcgt cggattgcaa atgaaccttt cagaaaaaga taccgtcatt acaagttacc
     3481 gcgatcatgg gcatatgctc gcttgcggca tggaagccaa tggcgtgatg gcggagctca
     3541 caggacgcat cggtggttat tcaaagggca agggcggttc catgcatatg ttcagccgcg
     3601 agaaaaactt tttcggaggc catggcattg tgggtgcaca agtcagtctt ggcacaggca
     3661 tggcctttgc gcacacttat aaaaatgatg gggctgtgtg cctcacttat atgggtgacg
     3721 gtgctgcaaa ccaagggcaa gtgtatgaat cttttaatat ggcggccctt tggaaacttc
     3781 ctgttgtgta tattatcgag gataataaat acggcatggg cacatcaact tgccgcgcgt
     3841 cggcttcttc tgatttttat aaacggggcg aaccttgggg gattcctggc atagaagtcg
     3901 atggcatgga tatttttaag gtcaaagaag ctggcgcaaa agcggtggag cacgcgcgct
     3961 cgggcaaagg accgttcttg ttgcatatca aaacctatcg ttatcgcggt cactctatgt
     4021 cggatcctgg gaaataccgc tcgaaagaag aagttgaaag catgcgtgaa aatcatgatc
     4081 cgattgcgca tttgaaaaat agcatgcttg cgaaaaaaat cctcagcgac gagggcttta
     4141 aggacattga ggtgcgggtg aaaaaaaccg tgaatgatgc ggcggaattc tctcaaacaa
     4201 gtcctgagcc cgacgctttt gaactttaca cagatgtgct gttgtaaaaa atataactca
     4261 aacaaaggag aatttttatg ctcaagaaaa tcgcactcac agcgctcatg ggctctcttg
     4321 gagttgtcgt gagcgctgtc acctcggacg ttaataaaga taactctggc tatgaaaaaa
     4381 gcctaaattt aacgtatcag ctgccctcaa aagaaaagat tgctgagatt ttagaagaca
     4441 gcggcctgca ttattttgat gaaaagacaa aatttagtca aaagaacgcg tggcaaaaag
     4501 tcacaggctt
//
LOCUS       DAIAHG010000091         4478 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_91_length_4478_cov_2.519655, whole genome shotgun sequence.
ACCESSION   DAIAHG010000091 DAIAHG010000000
VERSION     DAIAHG010000091.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91_length_4478_cov_2.519655"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1321 a          972 c          926 g         1259 t
ORIGIN      
        1 gcttttttca cacccatagc acggggcaac ttatttctca ttttacaaat gatgtgaact
       61 tgatgcgaaa tgctgtagcc cacaccatta ttggtcttgg caaggactca ttgacactca
      121 ttttccttat tatccttatg ttttatcggg atttttatct gtcgattgga acatttatta
      181 tttttcccct gatcttttat cctattttgc gccttggcaa acgccttcgt aaagtgagtc
      241 actccaccca agactatctg ggcgaactca cagggttttt aacacaaatt tttcaaggca
      301 ttcgcgttgt caaagcctat tgtgccgagg aatatgaaat caagcgcgcg gaaaatcgcg
      361 tcaaaaagat ctttcactat ttacaaaaag catcgattac acgggcgatt tctcacccca
      421 ttgtcgaaac gattggcggt tttgcgatta tgagtgtcat tgcctatggc gggtaccagg
      481 tcatgcaaaa aacccgcaca acgggtgagt ttgtctcctt tatcggcgca ctcattttaa
      541 gttatgagcc gatgaaacgt ctttccaata ttaatgcgaa tatccaagag gggcttgcgg
      601 cagccaaacg tatttatact ctttttgaca caccaacgtt ggttaaagaa ccgaaaaacc
      661 ctgttttact tgaaaataat cgtctcaagg gagacattcg ttttgaaaat gtaagcttta
      721 cttacggcac agagactctt gcattaaaaa atatttcttt ttccgtcgaa aaagggcaaa
      781 cgattgcttt tgtcggtccc agtggcgctg gaaaatcaac acttatcaat ctcattcctc
      841 gtttttatga tgttacagag ggtgctattt ttattgatgg cgtgaatttg cgtgattttt
      901 cctttaaaga tttgcgcgag aatatttctc tcgtgagcca ggatacgatc ctttttaatg
      961 aatctgcctc cgctaatatt gcgtacccct cagaaatttt cgatgaagag cgcgtgaaaa
     1021 atgcggcgaa agatgcggcg tgcgatacct ttattcagca ttttcccaaa aaatatgatg
     1081 agcttcttgg ggaaaatggc aatcgtctgt caggaggaca aaagcaacgt tttacaatcg
     1141 cacgtgctct ttataaggac gcccccctcc tccttcttga cgaagcaaca tccgctcttg
     1201 actctgagtc tgaaaaacaa attcaatcgg ctctgaaaaa tttgataaaa ggtcgcacga
     1261 cactcattgt tgcccaccgt ctttcaacga tcaaagatgc cgataaaatt tatgtgatgg
     1321 atggcggcga aattgttgag gaaggacgcc ataaggatct tctggcgcaa aaaggtctct
     1381 atacaaaact ctggcgcatg caaacaacgg agaaagacta aaaaattatc gttaatatcc
     1441 gatgttccta aaagaaacag tggggaagct cgaggacgat aatggtttaa acgagcccaa
     1501 attcttgaaa ttaaattgaa gaagcgctcc cgtatccggc ttaatatcac ggtcatgata
     1561 gcctgtgtga taaaaaccta aactcatctg aaaacaatca ttttcatata cagccgaggc
     1621 catatacgcc ctggcactgc gctcgatgcg ggacagattt tgcgtgcgcg taaaacccaa
     1681 agaccactca tccgtgattt tagacatcac ttgccagtta atttgattaa taggttgtcc
     1741 cgatggtgtt tcttttttat ccacatagat ataagacgta ttaagattta aaagaggata
     1801 accaaaattt gcccctactt ctgaaaaacg gttttgcact tttttgttat caaagcgacc
     1861 tctataatga aggcgcagcc attcaacagg aacacactgc aggcgcgtaa cataatctga
     1921 tgctttttta tgatccccga taagatccac accccgttta ttcaagcgat agctttgccc
     1981 taaaaagagc acgacacgcc ttccctgagc accataaaat cctgtattca caccgtacac
     2041 aaaacgtctt cccgaatcgg tccgatcaaa cccattcatt cggtcaatga aaaaaagatt
     2101 taaatcatca agctcgctaa aacggctatc ctcatttggg attttcccag gattaagccc
     2161 acttggcgag gccacaattt gagcgatagg ctcgatcaga tattgtccac ttcccatcat
     2221 acgcgctaag ggataacgcc acgtcaacgc agccgtgggc ataaagcgcc ctgttgtgta
     2281 gttcgaatgt gagcgctgaa aagttgtcgc atagtcatga atggcgtata gatcacccct
     2341 catttgcgta tcgacctcaa aaagatgtcc tgtttgtgta gtataaggaa gataaaatga
     2401 ccctttaaga atcccacgtg tgcttgcttt gggtgcaaca ttggtggggc taaggcgccg
     2461 tgagagtgtc aaaagattcc cctcaaaacc aaagatctgg ccgaatgtac ctggatcaga
     2521 cttataaaga tattgagcca agggaaaaac ataaggattc agtttagggc gatccgtttg
     2581 aaaggcaaag gacttcagcc caaaatagtg atttccttta aagcgttccc attttgcaaa
     2641 ggatgtaagg tttttatcct gagccacaac ctgtcgattc gttacaatat tatagcgacg
     2701 aagatacgtc gtatcactgg cacgctttaa atcaaatgtc acaagatcat aatccgtgag
     2761 ctcgaagcgc cctcttgcaa aaaggtgaaa gcgtgttttg ggaggtgttg gattattttg
     2821 cgtttctcgt ataatctgtc gattcaaatg gcgtgattgc gttaggcttc cactcaaaga
     2881 gaaatcgcct agcttaaaac gacgacgata ttccgccgcc ataatagggc cttgttttgt
     2941 cgtgataatc ggattaagcg ttagatcctg atgggggccc agaacaaaaa agtaaggaac
     3001 tttgacaatg gtaccaagat ccgatgattg cccataggtt ggcatcagaa aaccactttt
     3061 gcgctttact gttggatcag catgataaaa ataaggtgaa taaagaacag gaacatcgaa
     3121 aatatcgagg aacacatcgc ggtacaccat cgtatgctct tggtgatcgt gcgtcacatt
     3181 ctttgaacgt atctgccaaa gggctttggg ctttgtctga cagacctcac acggtgaata
     3241 tgtaatttta tgaaaagtag ttatgttacc gtccttgcga tcgccttttt ccgccgtaag
     3301 acgcccttca tgattgctca aaagaagacg cacagaatca ataaacccat tgtcaaaggt
     3361 ttcatcgata tcaactttct cactccaaag aacattttga tcttcatcaa tcgctatcac
     3421 attccctgtg gcaatgattt tttgagtttt gcgatgataa ataatgtgat ccgctcttaa
     3481 aaaacgctcg gatttagagg gatgagtagc gtgtgcatcc gtctcttgcg tttgaaaaaa
     3541 aagtggtgtt gcctcagtat ttttatcaaa aaactgactg atttcaacgt taccaaatgc
     3601 ctcaagagta tcaagcttct cgtcgtaaac cacacggtcc gccgtcatat acaaaggaag
     3661 cttctctgca caaagagaca aaatatttgc aaaaaggaga aggaagaccc acaagatcat
     3721 tgggtggcaa agaattatta agaagcgcta atctttcttg atcataggac actcccacga
     3781 aaacacgcaa atcaaatccg cccttgaacc ctttttgctc aaattaattt gtatttttag
     3841 aaaaattatt tatcataagg acatgataca tttttatata tcttgttatg caaaaaacaa
     3901 accgtagaga ttttctctat ctgtctgctg caagctttgg tgtagtgggt cttggcgcta
     3961 gcattctcac gctgattaaa acgatgaatc ccgcaaaaga cgtcatggcc ttagcctcga
     4021 tcgaagttaa tattacaagc ctcaaaccgg gtcaagagaa aaccgttaca tggcgcggca
     4081 agcccgtttt tattaaaaga cgtaccccag aggaaattaa aatagtcgat gcagttcctc
     4141 taaaggatct aaaagatccc caaacggaca aagagcgctt tcccaaaaat caggaattcc
     4201 ttgtcgttgt aggcgtgtgc actcaccttg ggtgtatccc cggtcaaaga gaggctctct
     4261 ctgtcaatgg ctataaggga cctggatggt tatgcgcgtg tcatggttcc gtctatgatg
     4321 attcagggcg cgtcattaga ggacctgcac ccaaaaatct cgcggtgcct ccttatgaat
     4381 ttttgaataa caatacgacg atcaaaattg gttgatagat atggggattg ttaaatggat
     4441 cgataaacgt ttaccctttc tctcctttgt gaagaatc
//
LOCUS       DAIAHG010000092         4396 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_92_length_4396_cov_2.670757, whole genome shotgun sequence.
ACCESSION   DAIAHG010000092 DAIAHG010000000
VERSION     DAIAHG010000092.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92_length_4396_cov_2.670757"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1131 a          897 c         1137 g         1231 t
ORIGIN      
        1 ttccccctgg atgaaaaagg ctatagtctt tgggggaaaa agcctttgcc tttaaaaggc
       61 acacagccag tgcatcgcca aggccaagca tcattattgt ggacgttgtg ggggcgagtc
      121 ccatggggca ggcttcggga acttcgggaa gaatgagcgc aacggttgcg tacgctgcaa
      181 gagcgcttgc tgggtttttt gttatgccaa tcacggggat tgcaaaacgc cgtgcataaa
      241 ccataagcgg cttaagttct tgggtttcgc ccgatgacga gagcacaagg agagcatctt
      301 cgcttgtaat catgccaagg tcgccgtggc ttgcttctgc ggcgtgaaca aaaaaagcag
      361 gctgacccgt agaggccatt gttgcagcaa ttttacgcgc aatatgaccg cttttgccga
      421 tgccagaaac gatgaggcgt cccttaatgc tttgaataag ggtgacagcc tttaaaaaat
      481 caccattaat cgctgtggcg acttgctcta aggaacgtgc ctcgcttaaa aggacgtctt
      541 tggcgtgatc aatcgctgtg ctttcgaggg gctttaagcg cgctgtttgt gacaaaagtg
      601 tgacctcttt ttcttttaat tttgcgaata ataatccact ttaatagcta agaacatcat
      661 aagttgatcc tgcaagaaca tcaagctggg cacggtaagg aagataatca aaagccgcct
      721 ttgcaagcgt tgcgcgatct tctcttttga gtgttttctc aaagtgattt ttgagcgtgt
      781 gaaggtgcaa cacatcggtg gccgcatatt ttttttgctc atccgtgagc gtttcagcac
      841 cccaatcgga agtctgttct tgtttagaaa tttctatgtt gaggatgctt ttgcaaagtt
      901 ctttgagccc gtgtttgtcc gtatatgtgc gtgtgagttt tgaggcaatc ttcgtgcaaa
      961 aaacaggtgc agcgagcaca ccaaaagacg catagagggc cgcaatatca aagcgtccaa
     1021 agtgaaaaat tttcaggatt tgaggatcac tcaaaagtgc gcacaagcga ggaggggggg
     1081 cttgattttt tttgatttgg accaaatgac agtggccatc gcctgtggag agctgcacaa
     1141 gacataggcg gtcgcgatgg acttcaagtc ccatggtctc tgtatcaacg gcgacagaat
     1201 caatctgttg aaagggatgt tgatcgggga ggtcgccttg gtgcaaaaaa atctgcataa
     1261 taatcctaat atgtttaaga aatcagcaga gcgttaacgg gggaaggagt caaaagatgg
     1321 tgcccagaag aagactcgaa cttccacggg tttccccact aatacctgaa actagcgcgt
     1381 ctaccaattc cgccatctgg gcacgatgat tatttctaga ggataacgta tcaaaagtca
     1441 attttttcgc gctgttcttt tggaaatttt tcaagggcat agcgcagttt tatggatttt
     1501 atcggcgggt tcataaagtg gaagcggttt ttgtacgccc aaaaaagaga attctataaa
     1561 atcgaatctc tagccataaa atcctcgcgc tgttcttttg gaaatttttc aagcgcatag
     1621 cgcagcatgg tgcgcggcat tgtggttttg tgagtgtgta aaaaagattc aagcgctaaa
     1681 acattgcgtt tgccggcctc tcgaagcatc catcccacgg ctttgtgcat gaggtcttcg
     1741 gtgtcgtgca aaagcttttc tgctaaccta aatgtccacg tgaggtcatg atttttaatg
     1801 aaggcccatg tggccacaat agctatacgc ctatcccaca gcacattgga ttgggcaagt
     1861 tctaaaagag aatttttatc acgatgcagt aaataatctc ctaaaataag gtgtgccgat
     1921 gcatccacaa gattccaatt attcacatgc gctttatgat tgaggtaaaa atcaacccat
     1981 tcttttttgt gatcctcaga agatttttga tagttgtgtg tcaaaatcac aagggctaaa
     2041 aaacgcattt cattgaaagg cgaggcaatg agaatgctaa gatcatcgcg cttcagatcc
     2101 ttaaaatcct tggcaatgtg gcggattttg ggaacaggga cccctataaa gcgatcatgg
     2161 gcaccgtaca tgccgggacc agttttaaaa aacatcgctg ctttttccgg cgagagcgtt
     2221 gcgttgcttt tgagaagggc ttcaatggtc gagatgattg cggtcatagt gcgtgctttt
     2281 tttcatgatc taggagccat tttttgcgcg ctcttccgcc tgcgtagccg cccaagtcgc
     2341 ccgtttgttg gcaaatacga tgacagggaa tgataatcga taaaaggttc gcaccgttgg
     2401 cgttggccac agcgcgacag gctgaaggat gcagcatggc cgcggcttgt gcgccatagc
     2461 ttcgtgttgt gccatagctt gtgcgctgta attcctgcca tactttttct tgaaaaggag
     2521 tgccagtgag agaaaggggg gtaagaaacg tttgagttgc cccggaaaaa tacccttgaa
     2581 gctcttttgt aagctgaatt gaagagggtg tttctcctaa aacaacggtc atagtgcgtt
     2641 tttggcaaag gcgttttatt tttatgtcct tttgtggcgc atctgagaaa tcgagaaaat
     2701 aaacagattt ctcatcgcaa agtgccacca tctctccgag tggggtattc agtgtggatg
     2761 cgtataaaaa acctggagtg gagaagggca cgagagcgag cgtttctatc tttggtactg
     2821 tcgtttaatt gtatacaaaa attaattgag agggtcaaag aactcttttg tgatctcttt
     2881 tttttaaaat aaaatccaca aacacaaagg catcggctat agtctcacca agaataatga
     2941 tcgtatcaaa aaaataagga tagttgttgt atgcgcgctc tcaaaagcct tcgtttttca
     3001 ggaaaagatg atattcttcc cttgatcgag ggaggaaaag gtatttcagt ttcaaacggt
     3061 gagagctctg gggcgtgggc agcagcgggc ggtgttggga cattttcagc cgtgaatgca
     3121 gactctttcg atgctgaggg gaatattatt ccgatgtatt accatgggaa gacccgtcga
     3181 gagcgccacg aagagcttgt caattatgcg attaagggag ggattaccca agcgcgtatc
     3241 gcctatgaaa catcgaacgg tcgcggtcgc attcatatga atgttttgtg ggaaatgggg
     3301 gctgttgaac ccattttgca tggtgtttta tctcaaacaa aagggcttgt gcatggggtg
     3361 acatgtggtg ctgggatgcc ttataagctt gctgaaattt gtatgcatta cggggttgac
     3421 tattatccta ttgtgtcctc ggcaagagct tttcgtatcc tttggaagcg tgcttatcat
     3481 aaatttgcag cgggtttagg gggcgttgtc tacgaagatc cctggcgtgc aggggggcat
     3541 aacggtattt cgaactcaga ggatccttta aagccggagg atcccatgcc gcgtgtgatc
     3601 gagctgcgtt ctgttatgaa tgagttgggc cttaatcacg ttcctattat catggcggga
     3661 ggcgtgtggt ggctgagtga gtgggcagat tggctcgata atgatttggt tggtcctatt
     3721 gcgtttcagt ttggaacaag gcctctttta acacaagaaa gccccatatc ctcggcgtgg
     3781 aaagaaaaat tactcacgct taagcccggc gatattaatc tcaatcgttt tagccccaca
     3841 ggattttatt catccgctgt gcgtaatcct tttttggcag agcttcaagc gcgttcagag
     3901 cgccaagtgg cctattctgt cacgtccgtt ggggatcaca cggacgcttt tcctgtgggg
     3961 gcgcgtgggc gttttgtttt ccttacgccc cacgataagg aaaaagcaga gttttgggta
     4021 tcgcaagggt ttacaaatcc tatgcgtacg ccggaaagta cgctgatttt tgtcacgcct
     4081 gagaaatcaa atgagatctt aaaagatcaa atcgattgca tgggatgtct ttcggcctgt
     4141 ttgtttagta attgggcaca aaatgagcaa ggcacaacgg gtaaaaaagc ggatccgcgt
     4201 tctttttgta ttcaaaaaac gctgcaatcc attagtcatc gcggtgatcc ggatcatgag
     4261 ttgatgtttg cggggcacgc ggcctatcgc tttgcacagg atgcttttta caacgatagt
     4321 tttatcccga ccgtcggtca gcttattgac cgtattttga caggatacta gaaaggagcg
     4381 ggagttttca ctcctc
//
LOCUS       DAIAHG010000093         4391 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_93_length_4391_cov_3.382244, whole genome shotgun sequence.
ACCESSION   DAIAHG010000093 DAIAHG010000000
VERSION     DAIAHG010000093.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93_length_4391_cov_3.382244"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1366 a          837 c          882 g         1306 t
ORIGIN      
        1 ggaagcgtga tttcatcata atgcccaata agtccccgac tctccatgta agcagatacc
       61 tcgctgcgaa ggcgaaagtc cacacatgta ataaggagga cctgcacccg ccgcaaaggc
      121 tcgctttgca tctccgaggg gtcctcaaaa ggagtctcaa aaatttgatc gtgcggagga
      181 tcttgatgat tcatcaagga tgtcatctgt ggggggtgag acatgttgtg gttgcgattg
      241 ccgtttacca tcgcctcctc cttttttttc agagatcata acgacgaaca agcccgatcc
      301 aatgacaagg atcgttccta cataaataga tagcgcaatt gtctcattaa acaagaggac
      361 actataaaga gtactccata aaatcgatga gtaaataagt ggtgcaaggg tattcgcggg
      421 ggtttttgtg tacgcaatcg tgacacatgt ttgtccaata cctcctccta taccgaaaat
      481 agcaaaaata atgagatcat tgaggggcag tgatacccac cctatgtgtc cagaaaatga
      541 actcacgatc atcgcaacag tcataagggt cattgaaata attgcatagt aaaatgtcat
      601 acgctcgggg cgttcacggg cactaaaaac ccggttgtgc accatcacaa gtccctcaaa
      661 aaacacagaa atcaaggcac acgtcacacc aatggtgttc acgtgacccg aggggtttgc
      721 aataattgct acgccagcaa accctgtgac aagggcaatc catccagaaa agggcaattt
      781 ttcctttaaa aagaaaaaag ataaaataca gacgatgaac gttgcaccaa aggacaaaac
      841 attagcatcc gcaaagggga gcaaaatgat agaggtaaaa agacagcaat gactaatcga
      901 tccgcaaatc ccgcgccata ggtgcgttgg aaaataatgg gttttgatag gaatacgtcg
      961 cgtgatcaaa aaataaacaa agggaatcat ggcaaaaaga ttgcgcagga aaagaacctg
     1021 aaaaataggg tactcttgct taatggactt aagactagcg ttgacagttg taaagacaaa
     1081 catgcctaaa agcataatca aaatgccttg tgtgtgggga cgtcgcatgc tttcagaaaa
     1141 attttttaaa aaagtcataa atttttttta actttaaggg agtattaaca cttttatcat
     1201 tctaatgaaa gtagggaaaa aaacaatcca cacattttga aaagcgcgcc cgtgttgtct
     1261 tttttttatc gtattttatt tattttttta atctttttgg tggatccagc ccatgctgaa
     1321 tctcttaaag atcatctcat atggttcaac gagaaaggaa aactgacacc gtgcggtgaa
     1381 aaggcgcttg cttttataaa gtccgcccca aaacatggtt tagacgccag tatttatgaa
     1441 aaaattcttc atattacaga gggcacatcg acagtcgatg ctgaacatat gatgacaaaa
     1501 gcgatggagc agtttgcgcg tgatcttaca ggtggggctg agcgtgatct ttttgcgcaa
     1561 aaacaccctt cgctgactct cattttaagt gtttccgata tcattaaaaa aggtattacg
     1621 agcgctaagg attgcactct tttaacgtat tttatgccac atcatgtttt gtataaaaac
     1681 cttcaaaaag cccttgcgat ctattctgat tacgctaaaa aagcccgaca agaaggcgat
     1741 cctgccttta atctgacaca aacccttaaa aaaggcagta tgggaaaaga tgttctttcc
     1801 cttcggcgtt acctagaatt ctataactat ttgacgccgt ctgaatcaac gaattctagt
     1861 cttttcgatg ataatgttga aaaagctgtt aaggattttc aagcacatca tggtgaagag
     1921 gccgatggtg tgattggtcc aaagacgcaa aaacttttca gtcgtactgc cgaggattat
     1981 ctaaaaatta ttcgccttaa tcttcagcgt tggcgctatc tttcacctct tgaggataaa
     2041 tgtattattg tgaatgtggc gggatattgg gtcagctgtc ttgaaaaagg gcatcaggag
     2101 ctttggatga aggccgttgt gggcatgcca agccgtaaga cacctctgtt taaagcaccc
     2161 ttaacacatt ttatttttaa tccaacgtgg accgttcctg atggtatttt ttataaagat
     2221 aaactgcctc gtattctcca agatcccgat tacttagatc gcatgagtgt ttttatgcgt
     2281 gatcatgagg gaatgcccat cgatccatca acagttgatt ggtaccacga ccgtccacgt
     2341 ctgacatatc ctccggggcg ttcaaatccc ttagggcaaa ttaaatttca aattcaaaat
     2401 ccttatacca tttatttgca tgatacaaat caaaaggagc tttttaagaa aaatagacgt
     2461 gcgctctctt cgggatgtgt gcgccttgag aaacctatgg aacttgtggc gtggctgagc
     2521 catggtgaaa aatatggaac agttgaagag gttaagcaag cacttgctgc ttcacatgca
     2581 acttcctggc acaccttgga acatcctgtt cccgtttata ttgtctatat cacagcctgg
     2641 atgggagatg atggcacagt ctattttagc gacccttacg acgaagataa aacggctttg
     2701 aagaattttt cttgacttaa attgtattgt aatttatgtg atggacatca ccacattatt
     2761 ttaattttac tatgatgcga tttatatcgt tatttatcct tatttttttc actcaagttg
     2821 ctctagcttc agaagaaaaa atacttgata ttcaagaatt cattcttaaa aaagagaaaa
     2881 caccctcaca gtatgcgttt tcgccgcaat tgattaatag ctgtcccttt gtaaatatta
     2941 tgaactttga tctttggcaa aaatataaag attcaaatga aagtgtgaac tatttcacaa
     3001 aaaaagagtg gcttgagaaa aatatcaacg cctttttagc gattcaagac tcagagctat
     3061 atctgcgtaa aaactcaaac gaatataaag aaatttttgt gaatgcttta aatggtttag
     3121 gcgaaaaaga gctctataca gaaaatggtg aatttattga agaaaactgg actggaagtg
     3181 ttgaagatct tatttctctc ttgggttatg ctccctattt tacaaaagaa gtgccctgcg
     3241 tctctacccc acttagtgtg attttaccgg aaactgtgat tttatcaaaa atacctacta
     3301 atcaagcttt gaatacagtc acatatgcag gggaatctca ctatgtcgtg agtggcactc
     3361 ttgaccctgg tgaaaaacaa acgattcact attctatggg aacgtatttt aaaatgcttc
     3421 tctatcttgg cattggcggc gagggtgaaa atcttaaaaa aggggatcca tactttaaac
     3481 tttcctctct tattaaagaa tcgattctca acacaaatca cccgtggaat tttttaacac
     3541 gtaaaaattt ctttgaatat gaactgatga aaatctttga aaaggatgag gatcttttta
     3601 aaaaagcgca aaaagtcatc gatataggtt ctttaaaacc ctggtgggcc tacaatgaat
     3661 caaaaaagta ttttacaaaa aaaggtaaga agcgatctga tgttttgcct ctgaaggatc
     3721 tgccgatatt tgcttatgat ttctcagcac ggcttaattc tttgtgggcc gccagcattg
     3781 ggaaaagcgc tcttcaggct gtgcttgagc ttgatgagaa tagttcgtcg gagatgcaag
     3841 aaaagtttga tgatctttat gattttatga cagcgcctaa aaactataag gcttttattg
     3901 gcgtgtggca tgagatcagg gagacgctca gcgctgaaga ctatatatgt tttttcggtc
     3961 atggtaacgt catgaccttt gctctcaaaa agtttaataa aatttttggc aaacagcagt
     4021 acgatcacga agaacttgca caagcccttg gttttatgaa aattgaatca ctttattata
     4081 aagaaattca cgctctttat cgatactcga attaaaggag gatttttatt tttcggacaa
     4141 tgaggagcat tttttgaggt aatttttgtg attttcggaa taattttgtt gttttccccc
     4201 gaatttttca cttttatggg cgtttgcata ttatttgagc gattttttca ccaaaaaagt
     4261 tgtatttttt cctcgttcga gcataatcgc accatttttt ctctgttttg acacgaaatt
     4321 ttagattttt gagccttttt cttcactatt ttgccaaaaa gccctaaaaa ttgctgttaa
     4381 acgtcccccc t
//
LOCUS       DAIAHG010000094         4235 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_94_length_4235_cov_3.505051, whole genome shotgun sequence.
ACCESSION   DAIAHG010000094 DAIAHG010000000
VERSION     DAIAHG010000094.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94_length_4235_cov_3.505051"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    249..258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1331 a          872 c          826 g         1196 t
ORIGIN      
        1 agaatcgatg cgtgagggaa cttatcgtta atggcttctt ataaaaaacg tgaaccaaag
       61 gaatcgcgtg agcttatcac acttacgagc gcgcacgcag gggcgagtaa aacaaaaaaa
      121 aattggacaa aacttaaggg atcctttcaa acgcgttttc cttttcggaa gaaagataat
      181 cgaccttttc cactcacttc aatgattcct aatcttgtga ccttgcttgg actttgtgca
      241 ggcgtaagnn nnnnnnnnga tttgcttttc ttggcatatg ggagtttgct gttgtcgcta
      301 tttttgtcgc ggcgatcctt gacacaatgg atggggcttt ggcccgtatg ctcagcgcta
      361 caagccgttt tggtgcagag cttgactctc tttctgattt tgcaactttt ggtatatccc
      421 cagcactcat tatgtatttt gcaagcctca agggttacgg cgcttttgga tgggtctttg
      481 ttttatggtt tattgtgtgt ggaggccttc gtttagcacg ttttaatacg cacagcatcg
      541 aaggaacaac gcccacatgg gccaagggct ttttcacagg ggttcctatt cctgcagggg
      601 cccttctaag cctcacccct ctgatgattc atttggcttt tccagagcaa catctatccc
      661 ttcatcctgc cgtatgtgca gttgttatgg ctgtgtccgg gtttttgatg atcagccatt
      721 tgcccacatt ttctgtgaaa aaaattgata ttgcccccaa atttctttta tatatcgtca
      781 ttcttcttgt gattacagca acagcgctta ttagctatcc atggcacaca ctaagcctca
      841 ttatgattct ttacgttgga acattcagtt ttagttatcg tgcctataaa aaactagagg
      901 ccgatgtttt aaaaaatgaa aattagagtg ttcaaagaga agctaacgat ttctaattct
      961 aaatttcaat caatatgatg agtttttaca aaatgcaaag gtgcacattt tccttcgcaa
     1021 gagtgccgtt gaaatctgag cagagttctt aaaagggtag ctgaacatat gtgagcagag
     1081 caccgtctaa cctcagattt agggacattg attgtgctcc atggattctc cggtattaaa
     1141 tttgtcccgg catttatttt ccataatact cattatagaa ttttttatct gtaacattca
     1201 aaagcccccc ctttacgaat aactctcttc ttcggatgaa atttcataga tgcaacctat
     1261 tgaagctttt cttaggcgat catccagata cgccgatagg tatggctctt cgccggtgtt
     1321 aaggggaact tgtccacgat gcaatccgcg ctttgacgat tgagtacaga agcttctgct
     1381 taaaaaaact tcagcgcagc gtgcgttgcc aatcgctggc tctactgaaa aaatacacag
     1441 cctttgacag gtcggagaga tcttttgtgg gtattgcccc ttgatacgaa acaacctctt
     1501 taacaggttt taaaatcgta agatggtccc cttcaaggta gccaagcttt tgaaaattac
     1561 taataaaagc acgaccttta aaggatggat ttttgcttgc tttgagaata tcaacagcaa
     1621 aatcactctc aaaatgaata cccaaaagcc caaaaagtat cgctggaata tcaatttgac
     1681 ttatcattgt tgaaattttc atgggtttta gaatttttgg cgcatagatt atgcaaggaa
     1741 tatggtgctt ttctggcgta atttctaagc gccctgacgt tccatgcgta tgatccgcaa
     1801 taaatacaaa aagtgtgcga tcaaaccacg gctttgtttt tgctttttcg atcaattgac
     1861 caatggcata gtcggcatat ttgacccccc ctgctcttcc cgttactttg ggagcaagat
     1921 caatttttcc ttctggatag gtatacgggc ggtggttcga tgttgtgaga gcaaacgtca
     1981 taaacggagt ccccgcatga taagatttgt cagcttctag aattacctta tcaaaaagat
     2041 cctcgtcaca aatcccccac gcattttcaa aatgaatttc atcttgttca aaatccttat
     2101 gttgaacaat ctcaaaaccg ttgtggaaga aaaaatgctc catattatca aaataggcat
     2161 tgcccccata gataaaacgg gtttggtatc cgtgcttttt aaagataaat cccatagatt
     2221 tgaggttttc gttatgcggc cgctttacaa tagattgccc cggtgatggc ggtatcgcaa
     2281 ggataagcgc ttcaatccct ctgacggttc gtgttcccgt cgcataaaga ttcgtaaaga
     2341 aaagtgattc tttgctaagt ttatccaaat gcggtgtaag attttcctta ttcccaaaag
     2401 tcgccataaa ttcagcactc atactctcca taaggacaag aatcacatta tatttttgcc
     2461 tattttcctt ggtttcatgc agtgtttctg ctttttcaga gggtgcatag gcgtcgatcc
     2521 ccctcgcctt cagatctttt tctgtgagat aaaattgatc ataggcgaga ctattttgga
     2581 aaaaggctga aaaaagcgaa aaaataccat tttgagcgat ttcacggtga atttgagagt
     2641 gtagtgtgct taaaatagtc tggggtgtac tgaaataaag cgctattgaa aaaaccacca
     2701 tcaaaagact catacccgca cgctttttaa aaggtggcac aggcaaaagg tgtgtttttc
     2761 ggctgaaata gagggataaa agaagaacag ccgttaaaat cacaaaaata attgaaaaaa
     2821 caatacctaa actataggat tccttgatat tgcgcaaaac ttctgttgta tacacaaggt
     2881 aatcaaccgc aataaaatta aagcgcgcgg aaaattcttc ccaaaaaaag gtttcagcaa
     2941 tagatgtaaa gatcatcaca aaggcaaaga gtgcaaaaaa aataaaactt attttccgat
     3001 ccagcccact tccatgcttt ttttctttta aaaacgtcaa ataaaggcac acaggtatga
     3061 gaaaaaatga taacaccgtc aggtcaaaca caaaacctat ggcaaaaatt ttaaaaaatt
     3121 ctataaccga caaatgctca acaccggcaa cagcaagtaa ataaccccgc gttaagagtt
     3181 cacacgcaag aaacacaatg gctgtttgcg acagtctctg tctaaaataa tgaaaagtag
     3241 cttgatcaat cattcaaaaa gaatcgcttg tttttaataa cgctttaaaa gtcgcgcaat
     3301 aacgacaata actaaccaac taagtagcat aattgcaagc gaatgcgaaa gaaaatgtgc
     3361 accttttagc atctgataaa ggctcatcat ccagccaatc attaatccaa cagcaagtcc
     3421 tattttttga taacgtggat tggagctcat aaaataaagc gccatgagcg caaaaccacc
     3481 gcttgcatgc cccgcaggaa aacattttcc gcgatcatat agatcatgca gcggcgaaaa
     3541 atcgagcgca cttcgaaatt cgcttggtcc accatagtgt aaaagccttg aggggcaatg
     3601 cacgtaagta agttccttga taagaacagc aagaagcggc gtaataataa tacttaaaag
     3661 aacgatagca taccctttat ggcgatcgtc taaaaaacgt ttttgataga aaaaaaagag
     3721 tatgttgagc aacagtccca cagcaaaaag agtaatgaca ttcttgatcc ctgtgtaaaa
     3781 aattatacgg agaacggcgt tgtcccttga aatcaaccat gtttttgaat gcggatcata
     3841 gaaaaaatct tgaatccaaa aatccaacgg agagttttca agccacaaga cgcctgcgca
     3901 cagaaaaata aaaatgaaaa aagagcggag tgacagcgtc atacaatttt agaaaaaata
     3961 atcgtcatcc acacataccc agcgacaagg atacttataa aaacagcagc gctggcaatg
     4021 tcttttactt ttttagaaag cgggtgttcc tcaagggaaa tgcggtcgat tgttttctca
     4081 atggccgtgt tcaaaagttc tgtcacaaga acaacaccat aagaaaaaat taaaagcgcc
     4141 ctttcaacga tcgtcaagtt caaaaaaatc acacaaatga gtacaagggg aagcatataa
     4201 atctcaagac gaaaagccgc ctcccctttg aaggc
//
LOCUS       DAIAHG010000095         4217 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_95_length_4217_cov_3.930193, whole genome shotgun sequence.
ACCESSION   DAIAHG010000095 DAIAHG010000000
VERSION     DAIAHG010000095.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95_length_4217_cov_3.930193"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1190 a          964 c          775 g         1288 t
ORIGIN      
        1 aatttttttt tatatcgttg tgtatacaat catcctcaaa ccacgcacac cgcataatat
       61 tgtgattggc ggattagccg gtgctttgcc ccctctgatt ggatggacag cggtaaccgg
      121 tgaaataaac ctccccccac ttatcatggt cgctattatt ttcttttgga cagtccccca
      181 cttttgggct cttgctcttg tcaaagccaa agactatgca caggcgaata ttccaatgat
      241 gcccaacacc cacggtcctt tgtccacaaa aaagcagatg tttttttata cggtccttac
      301 ggttctatgc gcatcgacgc tttggataat tggctttagt ggcctctttt ttttgaccat
      361 aagtctaggt ttaggaagcg tctttctcta tcttagctat gccctcatag cccgggaaaa
      421 agggcatgag atgcgccttt tcacctattc tattttttac cttttcgccc tttttctcag
      481 cattattatc gatcaaagga tatttttatg aacgatccta atcttcgcaa acgcaaccgc
      541 gcggtcctca tcgttcttgt tagcctcgct cttctgctct acgccgttgg ttttatccgc
      601 gtaagtttct gagagaggct gtcagtcacg cccaaagaaa attactccgc gtcttgagaa
      661 acttcatctt caggaataat ttcaaaatgt gcgtcggcgt attccgtcca ttttcccaat
      721 agagattcca tgcccatctt ccacagccgc tccagacgac gagtgagttt cttttcttta
      781 tctaaaagtg cgatcccttc cttaagctca tcctttgtaa ggccaaactc caggtatttc
      841 tcatcgattt tattattaat aatatcccag tattccttca aaatagacaa atttaacttt
      901 tttatctctg atgtaatctt atcttcttcg gctttagtct cctcaagatt tagtttaagc
      961 tcggctaaag ttttttgaag gtttttatac gcatcacttt ctggatcaag tgttgccagc
     1021 ttagaggact tgtcctctaa aagaccctcc aaaagatcta cacttctgga caacatatct
     1081 ttttgattag aaagtttatt ctcggtctca atcaggaact ctctctcctc atctacgtca
     1141 cctttaaagg atttaaactc ctcacttttt ctatattcgt tgataagaga gtctacttct
     1201 ttatactttt ttaaaagcaa aagatctttg tctttccact tattcatttc tttctcgagg
     1261 cgactctgca cataatgatc gcggtctcct ccttcttttt ccaatttctc aaaaaattta
     1321 attctttctt tatcatattc tttttgagtg agatgttttt tttctgtcgc ctcttgattt
     1381 atctcctcat tattactact ccaagaggat gtcacccccc ccccaaacaa cgcaagagag
     1441 agcgcaaatg ttttatacag tgtgttcata ataaaattcc catcaattca tcattaaatc
     1501 acttctagtt atagaggata aaaattaata gacaatgaat aatgatggga gatttaatga
     1561 aattttatta ttttggaaag aactcgtttg tatctcctaa aagataataa ttcgtaagcc
     1621 ccgcccactc agtacagact tgtttaaagg ccataggcgt gatgcgttgc cttatttgtg
     1681 caaaccaaaa agtgaaactc attttgcccg gagtatgata atctacagga taagggatga
     1741 tattgaaccc ttctttgcga aataagccaa cggcccttgg catatgaaaa gctgacgtca
     1801 caagaatata agttcctttt ggatcgataa gttttttaag aaaaacagcg tgatcttgag
     1861 tcgtgcggga atcagtttcg atcacaatat ggtgacgttg aatcccttct tcaaggaaaa
     1921 ttttctctgt ccactctgat tcgatcacat ttcctgttga aataattttt gcatggggaa
     1981 atttcttggc aagctcaata aattgataaa tacgtgttcc tgccatatta tacacaggct
     2041 cgccacgctc ttttgtttcc aaaagggaaa aacttccccc gagcaaaatc aaaccatcca
     2101 catgatctgg aagctctttt ggccttggga tacgattttc aaacttgaca aacatccaag
     2161 gggcgaaggg agaaaaatta ataaaaatta gaggaacagc ccctaaaaca aaaaaagtaa
     2221 gcgcttttcg gcattttttt aagattaaga aaacaagacc aaggacaaga gatccaagcg
     2281 ctaaaagatc gagattaatg aaaaaccaga aaaaatagta gagaatgatg tccataggat
     2341 ttttcttaaa aaataattaa agttgacata atacacttac accaggaagg ccacactctt
     2401 caagatactc taaaaaggcc ccccccgctg tggagacaaa acttagattt tttttatcat
     2461 cgccaagaca agctaatgta tcaccccccc ctacaagggt tgttgcccct tcttttgtac
     2521 gcttggaaag agagtgcaca atggacgctg atccctcatc aaaaggcttt ttttcaaaaa
     2581 caccaaaggg cccattccac acaatagtcg aggctaagct cactttatct tcgatcatct
     2641 gacgggtctt cggaccaata tcaacaatct ttagtttggg tggaacattc acaatatcaa
     2701 gggtcattgt cagtgtcgga tgatcaagat cgtccaccac aatcgcgtca atgggcagca
     2761 taaatttcag tggatttttc cgtaaaaaag cacccgcttc ctccaaaaaa tctttttcat
     2821 aaaaagacgc cccgggtttc aaaagacctg cggccacaag aaatgtattg gccatgcccc
     2881 cgcccggaat aatcagtgtg gaaagtttca agaggctttc aagaagtttc atctttgtcg
     2941 aaacctttgc ccctcccaaa agcgaaagcg tataggtttt agcgttaggg gcctcacccc
     3001 atcctttttc acaaaattgc ttgagatatg ttacttctgt aaggagatta taccctgcaa
     3061 tacgtacctc catgcgcttt ggaacaccat aaacggacgc atgtttacga tgactcacag
     3121 aaaaagcatc attcacataa atcgaacaaa gggacgctaa atctgcagaa aaatcactct
     3181 cattttcctc ctcgcccttc caaaagcgta tgttttcaag cagcacaaat tgaccctcct
     3241 caagggtttg aagcatttct tgcgcatgct caagggtctc tgaaaactta actttttgat
     3301 gtagtttttc ctccaaaaga ggtaaaagaa tttttgtact tagtgcctcc tctctttttt
     3361 gagggcgccc acgatgagtc acgataaggg ttttcgctcc tttttcttta agcgccaaca
     3421 gcgtttgcat ggatgcatcc aaacgtgagg tgtccgtcac gttcccctca tcatccacag
     3481 gcatattcag atccagacgg agaaaaacat tttttcccgc tacatctgca ttctctaagg
     3541 ttaaaaatcc acccatagca ttccttaatc tctaaaataa tgtgcccttg atgattctag
     3601 cagtttcact ttttagaaaa aactctcttg tgagatcaga tagccaccgt gtgctttgac
     3661 tttgagccaa agaaaaacta aaatcgggca ttcattcttc ttttttgcga gaaaactatt
     3721 gatgcgcgca ttcgtttttc caggacaagg ctcccaagtt gtggggatgg ggcaagattt
     3781 ttatgagcac tttaaaattg ctaaggatac atttgatgaa gtggatagtg ttttagcgcc
     3841 tctgttgcat caaaaactaa gcactattat ttttgagggg cctgcggaca cgcttacgca
     3901 aaccctttat acacagccag cccttatgac tgtcagcata gctatttata gaacccttga
     3961 gtacacactt aatgtagatc tcacggcaaa ggcagctttt atggcaggac actctcttgg
     4021 ggagtacagt gctctttgcg ctgcaggcgt gatcagtctc gctgacacag cacaacttct
     4081 ctattaccgg ggtaaggcca tgacagagtg cgcggcgata gattctagtg aagagggaag
     4141 ccccatggcg gcgattatcg gccttgagat ggacgtgcta gaagatctca caagcaaggc
     4201 cggttgtttt attgcca
//
LOCUS       DAIAHG010000096         4183 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_96_length_4183_cov_3.142474, whole genome shotgun sequence.
ACCESSION   DAIAHG010000096 DAIAHG010000000
VERSION     DAIAHG010000096.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96_length_4183_cov_3.142474"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1144 a          793 c          885 g         1361 t
ORIGIN      
        1 acgaagaatc ttttaaatca aaggcaaaag acaatcaagt ggcgtaaaaa tatgtttttg
       61 acattttttt cacttaaagc ttgcttatct tttttgaaaa gacgggcccc cttgaccatt
      121 tgtgtttgag gggtgagaat aacaagggaa cttatgatct ggagatctaa tcatttctga
      181 tggcaataca ggtatcgtct tttactcttc ctgattttag tgagctctcc aagcgaggcg
      241 atatcgcctt tgctcttggg ttgatgacga ttttatgcgt gttgattttt ccattgcccg
      301 cgattctttt agaccttgca cttgccatgt cgattacctt ttcaattttg attttgatga
      361 cttctctttt tattcaaaaa ccacttgagt ttagcgcttt tccaacggtt cttcttgtgg
      421 caacgatggc gcgactttcc cttaacgtcg catcgacgcg ccttatttta ggctatggtc
      481 atgagggaac gcatgcggct ggaaaagtga ttcaggcctt tggtaatttt ttgatggcag
      541 gaaactttgt cattggcatc attgtttttg caattttagt tattgtgaac tttgttgtga
      601 tcacaaaggg ctcaggccgc attgctgagg tatccgcacg ctttaccttg gatgctatgc
      661 ctggaaagca aatggccatt gatgcggatc tttcctcggg gctgattaat gaaacagaag
      721 caaaacgtcg tcgcaaagag ttagaggacg aaagtaattt ttttggtgcg atggatggtt
      781 cagcaaaatt tgtgcgcggt gatgcgattg caggcctcct catcacattt attaatattg
      841 tgggcggtat tattattggc gttgttcaga tggatatgaa ctttggcgat gcctcgcgca
      901 cctatacact tttgacagtg ggtgatggtc ttgtcacgca aattccagcg ctgattgtgt
      961 ctacagcagc aggtcttctt gtgtcaaaag caggcgtgga aggaagtgct gagaaagctc
     1021 ttttcctgca acttggggct tatcccacag ccttaggctt gagttctttt ttaacagggt
     1081 ttttggctat tctcccccat atgccaacgc ttcctttttt gcttttgtct cttgtgacag
     1141 gaggaacagc gtggagtctt gcgaaaaata aagaaaaaca gcgtgatcaa gaacagcaac
     1201 ttttagagca agaaaaagct ctggaaaaaa cagcacctga aaaagaagaa cctctccgtg
     1261 tgagtatgga tcatattaaa atagagctgg gttacggtct tgtgggcttg cttggtgaat
     1321 caacaggtcc gagcttaaca gatcaaatta aaaatttacg tgggcaactt gcgcgggaga
     1381 ttggctttat tctgccgtcc gtgcggctgc aagataatat gcatttagag agcaatgaat
     1441 atgtgattaa aatgaaagaa cttgaagcgg gtcgtggtga cctcaggcct atgcactatc
     1501 ttgtaatgga tccctcagga cagcctatcc gccttgaggg agagcatgtg accgagccta
     1561 cctttggctt acccgccctt tggatcactc ccagtcttaa aattgacgct gagacgcggg
     1621 gctataccgt tgtggataat gcaaccgttt taacaacaca tcttactgaa attgttaaag
     1681 atcacatggg tgagttgctt tctttttcag aggtgcaaaa gcttttggat gatatggatg
     1741 acgcgtataa aaagcttttg aatgacattg tgcctgcgca aatttccgta ggcagtatcc
     1801 aacgtgttct tcaaaatctt gtggcggagc gtgtttccat tcgggatttg acgagcattc
     1861 ttgaagcagt ctcagaggcc tgtgcttttt caaggaatac aaccatgata acggagcatg
     1921 tgcgtttgcg cctgatgcgg caaattacct atgcgaatat agatcacgat catatgctga
     1981 gtattgttaa tcttggggcg acatgggagc gtcttttaac agaagctctt gtgggtgaag
     2041 gtgatattaa aaatcttgcg ctaccgccca gcacgctgca aatttttatt acaaatgtga
     2101 aaaacatttt ggatgatctg atactccagg gggaaatacc tgtgctgctc acaacggcga
     2161 cacttcgtcc ctatgtgcgc tctatcttag agcgtgtgcg cccctcaacg attgtgatgg
     2221 ggcaaaacga ggtctatcca aaagtaaaac tcaaacacct cggccaagtc gcacctcctg
     2281 gatagatcta acaattacta atctttttta ttttttcttc tcgttgcttt ttttataacg
     2341 cgctcttttt tgtttccatt gatttctttt aaaatattct cttgttcatc agacatggtg
     2401 ttcaattttt ctaaatcgcc caggagattg atgaactcat tgtcttgatc agtgggaatc
     2461 tcagacatag ctttttttac attttgaaag ttttctttaa agattttgtt caattcatta
     2521 agagtattgt cattagaatt aatcttgtat tgattttcat ttaaattctt agacatattt
     2581 tcgttaattt tctcgagatt cttaataaga tcatcaaaac cacttgcaaa tgatgtgctg
     2641 gagagcgccg cggagagaag aataagtgca attttcatgg gatttccttt ttctaaaatt
     2701 taagtatttt ttaatataaa attaaatata aatgctatta atgacaaaat atattatgct
     2761 ttttacatgt taataaaatt aaaagtcaat atatttattt ttagataatt aataaaagaa
     2821 aattagactc acttttcagg aaaaaacttg cattttgagg taaatttagg tacacaatgt
     2881 cccatggtca catagctcag cggtagagca ctacgttgac atcgtagggg tcacaggttc
     2941 aatccctgtt gtgaccacca tctttggaac ttcttcgctc cttagaggtt ttttattatt
     3001 cttagatttt tttgggcatc acgatctttg ctgtaacttt aagaacatca cacccgtcgc
     3061 tcttgtaaat tttcttcatg gcctccttaa gatttttatc attaaggtca tccagaagcg
     3121 tccactcctc gtattccttt gtctctctcc ctggccaaat gcgttcaata tcaattgtgg
     3181 cgccatgctt aaaatattta gtaaaaagct ttaagaaaac cttctcaatc gatttaattt
     3241 ttctgtatcc ctctgcataa ggaacgaaag atccaccaag gatgagatat atcaaatttc
     3301 tttgaattcc tccatcaata ggggatgtac tctctgggtg taccgttaag aagagattga
     3361 aatgaacact cactttgtcc gaatctttta atgtgagatt cattttccaa ttctgagaga
     3421 gcgctttctc ttctgagtct tttttatcag tatcaatccc gtagatctga tcgtagattt
     3481 cttgaggtac gttttgccgt gcgtatcttt tatttttgtc aaaatatatt tctaatgctt
     3541 catttgcata aacaagctca agttttgtta tcttatcaat aagtgagata ttgaaaggat
     3601 cttcttcaga tttctcctta gcaatagaag tgttctgtaa aaacacgaat gctattaggg
     3661 cgctgtataa aattttttgt atcattgcct tctttttttc ctgttatcat gaatccctgg
     3721 tggcgacctt attgttttta aagcaccaat ctcttatata taaagaaaat aactgaattt
     3781 aagaaaagga atatgatcat gacgtgcgcc catgacagag acgcaaacgc atatgtagaa
     3841 cccgttgagg aaagtgctga aacagaagca gcgacgcctg ctcaggaaat caattggcaa
     3901 gagcaagccc tcagctttaa agatcagtgg atgcgcgctg tggcagagac tgagaatatc
     3961 cgcaagcgtg caatcaaaga gcgtgatgat gctctgaaat atggtgtgac aaagtttgcg
     4021 tcggatgtgc tttccattgc tgataatatg aaacgtgcgt tggataattg ccctgaaaaa
     4081 attccacaag agcttgaagg gcttattggc gggattaaac tcgttgagga tgaaacgaaa
     4141 aaaattttag aacgtcatgg cgttcgtgaa cttaatccca tgg
//
LOCUS       DAIAHG010000097         4166 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_97_length_4166_cov_4.224016, whole genome shotgun sequence.
ACCESSION   DAIAHG010000097 DAIAHG010000000
VERSION     DAIAHG010000097.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97_length_4166_cov_4.224016"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1173 a          944 c          864 g         1185 t
ORIGIN      
        1 aacggctctg gaaaatcaac cttactcaag atattagccg gatttattga ggccgatgac
       61 ggagatcttt ttcttcaacc aggtctttca gtagcgtatc ttcctcaaga tgtcgatctt
      121 cctggacacc tcactcccct tgactatatt agccagacgg gcattgatca ttatttagca
      181 gcaagttatc ttgaccgctt gcacatcgag gccgatcgtc cgatgcagca tctttcagga
      241 ggggagcgcc gacggattgc ccttgctcac acacttgccc aaaaagccga tattcttttg
      301 ctggatgagc caacaaacca tatggatttg ccggcgattg aatggcttga ggagtattta
      361 tccagctttc aaggtgcact gattttgatt agtcacgatc gctttttttt ggaaaaaaca
      421 agtaacctta cctactggct agatcgtggt gaaatttctg tcaataaaaa aagctatggt
      481 gattttgatc gctggagcga agagcttatg gcagaagaag cccgtcacct tgaaaaattg
      541 gatgtcaaac ttgcagcaga acaagagtgg ctccacagag gcgtcaccgc acgccgtaaa
      601 cgcaaccaag gaagactgcg caaccttttt gatctgagag cggcgcgcag agaagcaaca
      661 cttaatcaac ctggtaaact taagcttagt tcatcagacg gtgaaacaag tagtgccatg
      721 attatcgaag cacaacacat cagcaaggcc tttggtacga aagtccttgt caaagatttc
      781 tccacacgga ttaaacgggg agatcggatt ggtattattg gtgccaatgg ttcaggtaaa
      841 acaacccttt taaaaatgct tgtgaagcaa ctagaaccag atacgggaac agttaaaatg
      901 ggcgctaaaa ttgatcttgt gtattttgat caaatgcgca caacacttga gcttgatgaa
      961 actttgtgga aaaatctttg ccctacaggt ggtgatcaag tctatgtagg cggacaatat
     1021 cgccatgtga tgggctatct caaggatttt ttgtttgacg aacgccaggt ccagggcaag
     1081 gcaaatatcc tttccggcgg cgagaaaaat cgccttgccc ttgcaaaagc catggcgcac
     1141 cccagcaatg ttcttgtttt ggatgagcct acgaatgacc ttgatatgga tacacttgat
     1201 ttgcttgttg aaatgctgag cgactatgaa ggcacaatta ttttaatcag ccatgatcgc
     1261 gattttttaa ataaaactgt tacctccatc attgcagtgg agggcaacgg tcgtgtggac
     1321 gaatatgtgg gagggtatga cgattatatc aatcaaaaaa aaacgcaaag gcccgtcgca
     1381 aaaaaaatac aaaatgctcc aagagagccc tctaaagcaa cagcgactgc gtctcataaa
     1441 agattgtctt atcatcagca acgccttctt gacagtttgc ccgccaaaat agccgccctt
     1501 gaaaaggaaa taaatcttat cgaagaaaaa ctctcagatc cccattttta cacacatcac
     1561 ccgcaagcgt ttacaacctg ttccaatcgc cttgcagaac tccaaaaaga gctaaatgat
     1621 gcggaagaaa catggcttga gctagaaata aatgagtgag caagagattt tcaaaaaaat
     1681 ttccataggt ggactcacaa aggatcaatt agttacgcga ctggttgagg caggtattca
     1741 gtttaatgag tatgcaaaaa tactctttga tcatccgtct ttttccccta gccatacatc
     1801 tgaaaaagtt aatttagcta aaatcacact atcggacttg ggtatgggta atccatgctc
     1861 ttttcgggaa attataggtc gcgcttcagc tcttggctta gagctttgcc ctctctattg
     1921 cgctacattt ttgagattag agtatttgga tcaaccagaa gggccatatc ttacagttat
     1981 ctctgctaaa cctcaacatg atgaaaacta tccgaatggc ttttatctta gaaattttaa
     2041 taaaatactt tggttaaggg gatatcgcgc cacagatttt tgtgagtggc caatggacaa
     2101 tgagtttatt tttttaaaat gactcactga agataatata aaatcaattt tttatatttc
     2161 tattgagatg cataacttat acaactgagt ttgataatct tacgtggtaa agattttttt
     2221 agagaggcaa aagactaaaa tctcttgcct ctcttcgcat ttcgccttag taatagacaa
     2281 tattcttttg agatttgatg tcatagtgaa cgaatcttta tcatcgcgca tcataaaaaa
     2341 actccgcatc atcgaggaca cccgcaataa tatcagcttg tggggagtgg cgtggatgag
     2401 ctttttttgg agcacagctt cgctgatggc tttctccatt ttgcctgctt ttttaacaga
     2461 cgtcctccat gcaagtaaaa caaaaattgg ttttatcgaa ggtaccgcca tctttatttc
     2521 tttcattgcc aaaatttttt ccggcgttct cagtgactat tttaaaaacc gtaagcccct
     2581 cattattatt ggatcgattt ttagtattct cattaaacct atgtttgcgc ttgcagccac
     2641 agtgaatatg atttttgccg cacgatttat cgatcgcctc agcaaaggaa tccgttctgc
     2701 ccccaccgat gcgttgatcg cagatttgtc tcctaaaatt caccgcggtg caagttacgg
     2761 tatgcggcaa tctctttata cctttggcgg gatgttcgga gccctgcttg ccagtatttt
     2821 aatgacagtg acaacgcatg attatcgtat gatttttatg ctttctatga ttcctgcaac
     2881 actcgctgtg attatcctca tttttgttgt gaaacagccc gctatccttg agcacgggaa
     2941 aaaagttgat tggcatatta aagatataaa atttctccca ccacgctatt gggcgcttct
     3001 tggtgtagtt tcgctgctaa tgctcgcccg ttttagtgag tcttttgtgg cccttcgcgc
     3061 caaagaagcg ggctggtctg tggcctctct tccgcttatt atcgttctta tggatattgt
     3121 gcatgcaagt atggcctttc ctatgggacg cctttctgac aaatttaatc gatacaaaat
     3181 gtttctcaca gggcttgtcc ttacggtctg tgctcatgtc acaatgattg cagattttaa
     3241 ttcagccatg atttttctgg gtgttatttt tattggtctt tatttgggga ttatgcaggg
     3301 cctcagctcc acacttatcg cagaggcctc ccctccccat ctacgtggaa cggcctttgc
     3361 gatttattat tttacagcag ggacatctgt tctgattggt aacgtgtgcg ccggaaagtt
     3421 tgcagatctt tttggtcttg tgggcgcttt ttggggcggt ttgggcttta cgctcgcagc
     3481 aattatagca cttttcgtaa tgatgcgcgg gatgaatcac cggtagcgtt ggagaatttc
     3541 tctccaaaaa gggataacgc gggcatagtc ctgcccctgc ggggcccacc acgtccccaa
     3601 atttgatggc aaggcccacc cctcgctcca cctcaacatg actcgcccga ctccaaaacg
     3661 gcaccgtttc gaaggcatca gaaaaaggtt gcccttttca ctcatgaggc ctatcctgca
     3721 gaaacgcgct tccgcatgca aggcccggcg tccgccacgc acgccgcttc acggcatgtt
     3781 tctgaccacc agactccact ttaccacccc aaaggttaat attctcttaa cacgccccaa
     3841 aaaaattcac caagcgagcc gctcaaatcg aggcattttt tcacacaatg ccgtcaaaaa
     3901 ccactaaaaa tgggaaaaat gccctaaaaa cacccaaaaa tcactaaaga cacgcccaaa
     3961 cgatgaaaaa atcgatgaaa tatcgcctca agcaggcttt tttaacggaa attctcgccc
     4021 tcatccctga ttttttcaca acgatgcccc aaaaatcact aaaaatggga aaaatgccct
     4081 aaaaacaccc aaaaattgcg caaatacgag gtaattatca tgaaaaaagt tctataaagg
     4141 cgaaatatcg cctcaagcag gctttt
//
LOCUS       DAIAHG010000098         4129 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_98_length_4129_cov_2.703110, whole genome shotgun sequence.
ACCESSION   DAIAHG010000098 DAIAHG010000000
VERSION     DAIAHG010000098.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98_length_4129_cov_2.703110"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1250 a          734 c          867 g         1278 t
ORIGIN      
        1 gatttcatcg attgttattt taaagaacat cattttcatt tgaaaataga tggttcttct
       61 tgacattttt caaacaatat cttttgatcg gggtatcgat ttttaaaaaa gggttcgcct
      121 tatgttttta cattctcttc tccagcgtga tgcttggagc tccaatgttg tgcaggttct
      181 aatcggtttg agcacccttt ttctcacatc acagatttct attccactcc atcccgttcc
      241 tattacactt caaacagtgg gggttatgat ggtggctctt tctcttccac aaagagcagc
      301 gcttcagtca attttttcct atgtagcttt aggtgcactc ggtcttccta tttttgcagg
      361 attttcaggg ggttttgcaa agcttttagg tcctacggga gggtatctta tcggttttat
      421 cgcagccgtt tatctgatgg caagggcaaa gacttttctc ggcatgacat caggttggcg
      481 tatttttatt aactgtgcct ttgggcaact gtgtatttat atctttggta ttgcatggct
      541 tgcttacgtt ctcggatctt tggataaggc cataacgctt gggcttgtgc cctttatttt
      601 tccggggatc atgaaggcgt tccttttggc cttttctttt cgttatattc acagaaaata
      661 gattacaatt gaaaacttca aagaaaccct tgcgttgatt cagtcgggta cacctattcg
      721 tgtgactttt tctcttgata atatctgtgc gccctgtcct catcataatg gaagagtttg
      781 tcagcagcag tcttttattg aggatcttga tacgcggcat gcaaaagccc tgggtcttta
      841 tggtggagaa acactaacgt gggaagaggc attagggcgt attaaaaatg ctattacggt
      901 ggatatcttt cataaaattt gtgcaggatg ttcgtggaaa gaatgggggg tgtgtgaaaa
      961 atccttgata aatcttcgtg aagaaagtga taagtaaaaa ataagttttc ctattcttta
     1021 aaattttcgg ccatagcatc aagtccaata agaagtgtgt agacctcaag atcaggaaac
     1081 tccttttgaa tgatttttcg tgcttcttta aaaacttctt tgtgtgtacg cgtttcctcg
     1141 tcccgtgtgg cgatgcttgc ttctcccttt aaaagtttaa aagcaccgca ttggcgatga
     1201 tcgagaaaaa taatgcgttt aatatggtga agtcctttga ggataccaat aacttcttca
     1261 agcgtttgat tccagtctgg ataggtatgt tgagtaagag caagggctgc acccggcaaa
     1321 gcgatctcat cataatcatt aaggagtttt aggttttcgc tcatcagacg ctctgtttct
     1381 tcgcgtaagc gaaaatcaac acaagaaata aggagtgtcg aggctgtgga tttattttca
     1441 gcacctttaa gagcgcgtac ctggatatat ttatttttta tagcttcatt gaaattttta
     1501 gcatggtgca cttctggcaa aggctctaag aaaaaagccg atcctaatgt caaaaaaagt
     1561 gttttagaaa aaagttcaaa catcaagagc agccatttat aaaggtctca gctctcttta
     1621 atttatcgct aaaaaatgaa tattttctta agaacctatt tttattaaaa atattttttc
     1681 aaatgcctga gaagggcatc ctttgagatt gaaaagtcgt tgtcgcacca gtatttttct
     1741 aaaagcctta atccctctcc aatgcatttt ccctgatagc caagcccttc gatatcgttt
     1801 gctttaatag gaaaaagagg aggaggggtg tgttttgaaa ttttacaaag ctccgccgaa
     1861 caatcatcac cgaaaacgct ttccctcagg caaagaaaat gcgctgcaaa atcgtcgcta
     1921 taacggtata aaatttcttg gatggaaggg gattctttga agcacttttg gatcttagac
     1981 catagagttt gctctttacg ggtgagcaca aaggattttg agatgatcga tttttctact
     2041 gtatgataaa gagcatacag gcgatagaga ggctcaacgg gggagggggg gagtttatat
     2101 ttttcctcca gagcgatcag ttgactgagc tccttaaaat gcgcatcttt ttcaatgtgc
     2161 gtgaaaatat ttttctcatg cataagagaa agaatcgtat gtgcacgtgg tgcggcgagt
     2221 attttaaaaa attcggagcg tatgcgttct cttgacagga gggatatgtg cgatgaaagc
     2281 tgtgtgcaag cccttagact ttgcgggtct aaagtttctt tgccaaaaaa agcattcata
     2341 cggaaatagc gcaaaatgcg taaataatcc tcatggatgc gttctaaagg atcaccaata
     2401 aaacgcacac gtccgatttt aagatcgtgt tctccttcaa aatagtcgta aagggtgcct
     2461 tttgcatcta aatagagagc attcatcgta aaatcgcgac gtcttgcatc ctcttcaaag
     2521 gaatcagaaa aagcaacatc cgcgtggcgg ccataggatt tgacgtcact gcgaaggctt
     2581 gtaagctgaa aaacattatg agtaaaaaca gctgtaatcg tgccgtgctt gatgcctgtt
     2641 ggaataacat gacaatgatc tttgagagcg gtaatcattg cttgcggatc ggctgcaacg
     2701 gcaatatcaa tatcatgaac gggaaggttt aaaagggcat tgcgaacggc tccaccgaca
     2761 attcgagctt ctatgcctct atcttttaaa agggcaagaa cctctttcac atgaggcata
     2821 tcaaggagaa aatgcagaga atgtgtcact gctttgcatg aaatggcgcg cccaagaaga
     2881 gtcgaactcc taaccttcag atccgtagtc tgacgctcta tccaattgag ctatgggcgc
     2941 atatcgttcg atgtgcacaa actacactaa aaaggtgttg aattgcaaca aaaaatctta
     3001 ttctttatca ggaaaataaa aagcttttca cgagatggaa aatcatataa acagtaaaag
     3061 ttatttgaat aaaaaaaggt taattaaaaa tgttttataa atttgctctt tctttcactt
     3121 tttctgcatt tgtttcgagt atggcgtgtg cgagtagtct tcagtttttt aatcaggaag
     3181 gcacgggcac gctttgtaat tttaccactc taaaagaagt ttcttactat ccaaatttta
     3241 acggtttcaa tgactataaa agtaattttt tttcatcaga gtgcaaaaag actgagctga
     3301 cagaaaaaag acaaactcag ctcaatgaag tgattcgcaa cacatttttt aataaaaaaa
     3361 ataaatttat ttatggaaat aagaaagctc gtctagaaaa acttgagaat cagcttcaaa
     3421 gtggtcttct caaacagggc gaactcctta caacgtatat ggaggggaag aaaaaaggca
     3481 aaatttgcgc gatgacatat tctttggatg ttgaaggcaa ccttgttgtg acaaaagagg
     3541 tacgacatct tatcaaaaac cgcaaagatt ttaagataat tgataaagat gaggatggaa
     3601 aatatacaat aagagaggtg aatgtgtatg aacaacaaaa tattaataat tctgactgga
     3661 gcctttggaa tacgcgttta ggctcagcaa cgcttgttaa gcgtgaattc agcggtgttc
     3721 tttcacaaac gacttttgat gatggtaaag tatgcaaaac atttttgagt ccctgctctc
     3781 cttattggcc taaagatttt tggaataaaa atgaggatga gattgatggt aagcgcatga
     3841 cagtcgagaa aaaagaaata caaaaagaaa taaaacagac tctacataca acggaacagg
     3901 aaacaatcac ttggtttggt tatttaaaaa agatgtggaa tagtctctca agctaaaaat
     3961 aatcaccttg tgcttcgttt aagagctgct aagattgagt atgtagattt tataagaaat
     4021 atactttatg aacttcgatg ccgctcttaa acttcttttc acagatgccc gcacgcacaa
     4081 tgggtggttg gataaacccg ttgatgatga tcttctcata aaaatttat
//
LOCUS       DAIAHG010000099         4076 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_99_length_4076_cov_3.062266, whole genome shotgun sequence.
ACCESSION   DAIAHG010000099 DAIAHG010000000
VERSION     DAIAHG010000099.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99_length_4076_cov_3.062266"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1177 a          804 c          825 g         1270 t
ORIGIN      
        1 taagggtggc ttagaatttt ctcacggcac agaatggttg caatgtctcg aatgctagac
       61 cctttaggga taaaaatttc actaccttca ctattttctg tctgcccaaa ggggccactt
      121 ggccctaagg tgacgtagac actgccaatt acaaaagcaa ccagcacaca aaagaaaaaa
      181 agacgaagaa tttttcgaat cacaaaagaa agtacatttt cttgggtaga tctccagatt
      241 atagtctact gaaaaaacaa aaagatacaa agggggagat catgctcaac acaattttta
      301 gcattacccc ctttggtcga aaattattac aagactcaaa gattttgcac cctataataa
      361 tattttaaaa acatggcata ttcgtcattt ccttgaattt cttgaagatg aacaatagcc
      421 tcttgagctt ttttattgtg ggcttgatct gccaatataa aaagttcatt ccacacctct
      481 ttaggatttg acttctcaat cccaaatagt tgagccagag ccttgtggga aggattgatt
      541 ttttcctgtt taatcgccat taaaagcgcc cctttaagcg catcagccca tgtatcgtgg
      601 gcataattca aaacattttt agaaggattt tttggaaaaa gaaggcgcat taatatgctt
      661 tttcttttct ctgctttcca agtgtgcgcc cccaactggg tcacttgaag agccgttgtg
      721 atcatattac gcacggattg ggccccatcg acatcgccat ttttttctaa cttttccaaa
      781 agatcttcag tagccttcca gcgatctgaa ggctccattg tgtacgaata gctgagtatt
      841 ttttgaagca tatcttcatt attctcaaag aggtccccgt taatctttgc aagatttttt
      901 agcgcagatt gtatgttctc ataccgtttt tcttcctcaa gctcatattc ttttgttttt
      961 ccgatatctg caaaccaatt gacaagctca ctgtaataaa catagccata tccttcacat
     1021 tctatagtcg cataaagagc cattttttct aaaatcttat ccatacgatc attttcatga
     1081 atgattttgt ccttataaag cttttcaagg agtgattgac ggcgtttaga tgaaatcgga
     1141 tcacgggcta agacccctgc gataaagcta tgggtttttt taaggttaaa ataatttgag
     1201 gggatgtctg gaaactcctc atcgggagtt gatgcctcta caacgagctg aatatgtacc
     1261 tcaggaggaa tgctctctaa ccctaagata tctgtgacat cgcttaattc acttatatcg
     1321 tgcatgcttt caatttttag gccctcaact gctattttga gaaatttttt ttcgcaactc
     1381 ttagtgtcga tttttttatt ttcaaacaat tcgagcactt ctttgagtga atccgtaagc
     1441 agcgtttgat tcccttcctt tgtggcacct cttaaattat taaaatggtg gcggaaaaaa
     1501 acaatttttg caggattaaa accagcctgt gcgaggtcca atacaagttt tagatgtgta
     1561 ttatctttta aacagttttt gggatttttt tcaatctcct cgatataaac ctttgcgagt
     1621 ttttcgtcct caagcacaag agggtcattt ttgttacccg gtttgatggc gtacacagca
     1681 agattgaaaa gagcttcctt cttttgaaca tattgaaaaa ggcaatattg ccttttttta
     1741 gacatttctg tcttactgtc gttaggatta attttgctta attttttttt gaattttttt
     1801 aaaaaatcat cgggattact atcgatggct tctagaaggt cctcccaagc tgttttgggc
     1861 acttcatttt cagtcgtgtt aaagatataa aaattttttt ctgtcattgt atccccaatg
     1921 gaccaatagg ttttgcgcat gaggcgctgt gtttttgaga tttcctcggt aggtcttttc
     1981 ggtttttcaa tctctataat ttgcttattg ttttctgttg aggcctgtaa ggaaggtcca
     2041 aacgaagtaa gagaggcaag caggaaaagt gttatcgtct ttttcataaa aaatccccca
     2101 agacacatga gaagatggtg cacaaaatcg cgcaccctta tagaaaaagt ctatcaaaaa
     2161 attttaaaaa aataatagta taatatgata tttaagtgtt ttttgctcta tttcgttcta
     2221 aacaaacgat cacctgcatc tccaagtccc ggaataatat aggcatgatc gttgagtttt
     2281 tcgtcaaccg ctgctgtaaa aatgcgcacc ttagggtatt ttgcactgac cgtacgaacc
     2341 ccgtcgataa cggagacaag ggtcatcaca ataattttct caggagcaac tccccgcttg
     2401 acaaggccat caatggctgc caaaagagaa tttcctgtgg ccaacatagg atcaacaatg
     2461 ataaatgttt ggttattgac cgtcggtatt ttaaaaaaat attcaatggc ttgtttgctc
     2521 acaggatcac ggtaaagacc tatgtgcccc tgacttgctg ttggaatgag ggtatgcaga
     2581 ccatccgcca taccaaggcc cgcgcgtatg ataggaacaa taacaatttc attttcgtcg
     2641 attgcaaacc ccttggcagt ggccacgggc gtttcgacaa ttttttcttt cacagcgaga
     2701 gagcgcgtca cttcatagcc taaaagcaag gcaatctcac ggagaagttg tccaaaaaga
     2761 agcgttcctg ttgctttgtc gcgcataatc gtgagtttat gttgaattaa ggggtgcgtc
     2821 accacggtta agtttggaaa ggcttgaaga atcgagggag atggagtgac agaagctgtt
     2881 tttgagggaa ggaggccacc ttgcacggta ctatttatca aaatgaaaag agagagggat
     2941 tttgccagag tccttagcat agaaattttc ctgcttttaa attaatatta gtaaaatttt
     3001 tatcataacc ctctccaaca aggtagaaac tttttagatt tttttgctca ggctgcacgg
     3061 gactgtagaa gaatttttta agtttgctcg gcgcattgaa aaaagagtgg tggttgtttt
     3121 tcgcttttag taaggaaatt ttcttcttag agcgatgttt ttcagtttcg tataaaagtg
     3181 caggcctttc tcaccccctt gacaaggatc ctttcttgtt ttaataatat tagcactcgc
     3241 tatgtatgag tgctaacaac gcctaactta aacaactaac ctataaggag tgttcgatga
     3301 cagtcaaatt caaacccctt cacgaccgtg tgcttgtcaa gcgcattgaa tccgaagaaa
     3361 aaacaaaatc tggtattatt attcctgaca ctgccaagga aaaaccccaa gaaggtgaag
     3421 tcattgccgt gggctctggc acacgcggtg aaagtggtgc tcttcacccc ttagatgtca
     3481 aagcgggaga tcgcgttctt ttcggcaaat ggtcgggcac agaaattaaa attgatgggc
     3541 atgattttat cgttatgaaa gaatctgaca tcatgggcat tttagctcac taaaaatgta
     3601 tgagtattaa ttgtaaaaaa gaaaaagagg taaattatgg ctgctaaaga tgtacgtttt
     3661 tccactgacg ctcgcgataa aatgctaagc ggtgtcaaca cacttgctaa cgctgtgaaa
     3721 gtcacattgg gcccaaaggg acgcaacgtt gtgatccaaa aatcctttgg ttcaccccgc
     3781 attacaaaag atggtgtgtc cgttgcaaag gaaatcgagc tttcggatcc tttcgagaat
     3841 atgggtgcac aaatggtgcg cgaagttgca agcaaaacgt ctgaccttgc aggagacggc
     3901 acgacaacgg caactgttct tgcgcaatcc attattattg aaggtcttaa ggccgttgct
     3961 gcgggtatga accccatgga tcttaagcgc ggtattgata tggccgttga ttccgttgtg
     4021 acggatcttc aaaagcgcag taaaaaagtc tccacgaatg atgaaattgc tcaagt
//
LOCUS       DAIAHG010000100         3926 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_100_length_3926_cov_2.543778, whole genome shotgun sequence.
ACCESSION   DAIAHG010000100 DAIAHG010000000
VERSION     DAIAHG010000100.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100_length_3926_cov_2.543778"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1233 a          776 c          764 g         1153 t
ORIGIN      
        1 atctgttcct atttattatc gtttgggtcg ggatgctact gaaggaaaaa ttaagatttt
       61 ggatgaagaa ggaaaaaccg ttcaagagat tgatcttaaa aaaacagaaa aaggcgatca
      121 taaaatcgaa tgggatcgta gccttaaaga tgttgatcca acggaacaac tggctgaacc
      181 tggtgtttat tacattcagt ttacagcagt ggataaggat aaaaaccctg tagaacatat
      241 tgttgagctt gaggggattg tgtcccaggt tgattcgaat gaaaagggta tcgattatta
      301 tatttcaggg atcaaggtgg atggtcgcat tacaaaagta tccatgccga atcaacagga
      361 tatgggggcc tctattgcaa agcatctcaa agatatcaag ggagatttaa caatcggaaa
      421 tccgattacg cctgatcaga tactaatcaa caatgataca aatattattg atgttaatat
      481 ataaaatacg aggactctaa ctatgtcaaa tcttgattcc gtttttaata ccttccaatc
      541 gggtgccaat gcttatggca ataagtttac agatcactct tataatgtcg caaactctgg
      601 gacgattgca ggaaaaaaaa gatcgagtta tatctctaca ttaaattcaa tttcaacccc
      661 tacaagtttt tctccagcgg gtgttgccta taaaccgacg attgatattg agacaattgg
      721 aggtgtgaca ccgacaacat caacatccaa ctttatgatt aatgcacagg ctttgtgtat
      781 tacaaaccaa tcgccaagca actcaactcc agggcaatat ggttttacaa aggtaggaac
      841 ttttgaacct gatgccaatg gaaattttgt caaccacgtt ggtcaatttt taaaagttgt
      901 gatgacagat gccaatggga atcctcttat tccaaatatg gcagcctttg accagcttca
      961 aacgctaagt attcaaaata acgtcggcct tcctctccct acaacttata tgaatttata
     1021 cgcaggcctt cctggtaacg ctgctattgg tgcgaattat tctcaggcaa aacttgttta
     1081 tgatgcgaaa ggaaacgaat ataacgttgt aatgtcctat acaaaaatag catccgttgg
     1141 aacagatcct gtggatagtg agcgttggca aatgaaagca atagcagaaa caaatacaaa
     1201 tcctcctgta gctcttaccg taaatgcggc atggacgaat ggtgtagata ttgtctttac
     1261 gaacggtgtc cctgttctta ttaatgatct tgcgggtgtc tcgcaacctg tgccacagct
     1321 gagcattaca cctcctgcta cggctgcagc agttcctatc aacattaatg tgaatccagg
     1381 agatgtcaat acaagtgacg gtttaacaac acttggaggg cttcttttta acaataaaga
     1441 tatgcaagca gatggtaaag ggccaggaaa ttttcaaggg tttcaatggg acaatgccgg
     1501 caatggtatt gtgagttatg ataacggtga aacgcaaatt atttgtcgtt tgccgttgat
     1561 tacttttaaa tcaatgaata atttagtcga gggttcaggc ggcgttttct atgcatcgaa
     1621 cgacgcagga ccgtatacca ttcgttttcc tgcacaggga gaaatatacc cttctgcggt
     1681 tgaaggatcg acggcaagtg ccacagacac gtacgtaggt atggcccaag atggcaaacg
     1741 ttttaatgcc tgtatcggcg gtcttggaaa aacgattgcc atgctcgatg ttctcgaaaa
     1801 tttgcttagc cgaggttagt tctctcaaag atactaaagc tttgacggaa tgatattctt
     1861 ttctttatcc tcataaagag ggattatttt tccgtctttg tagtccataa cgataaaggc
     1921 tgcagaaaaa acctgatttt ctttgctctt atcatcatta gtgttttgtg aggacttctt
     1981 atacccctca acaatacatt taatcgatgt gttatcatga ggataaagtg ttgcccaaag
     2041 ctcaagaata ttccccgcac tcataatatc atcatgaagt gtaatatgag actctttgat
     2101 cataaagttt gtctttttct taaggagatt cagtgcttca aaagcagtat gacgcgcata
     2161 ttcaaaaaga ttcggcttcg ataacttctt atgctcatta agattatcct tatctattgt
     2221 ttttgtatcc atgaggtaac ataatcccaa ttctattttt ttctctggct cttttttctc
     2281 tgaaaaccca agattttgtt tgttcaaagg aagagagtgt gccttaccat tcttatactg
     2341 aacaaacgta aagagtcctt ttaccacagg aatagggttt tcaacattct ttcgtccaca
     2401 tatacctaca aaaagacaat ccatagaagt ctttcccgtg cgcacaattt tcacataaat
     2461 agaaagttca tcggcaactt taatttcttc aataaaatca atctcagatc gtagtgtcgc
     2521 aacatctcct ccacaatatt gtcgaagcgt attgacgatg ccattatcaa tacgcgtaaa
     2581 aatagctggt cctgatgcga cattattttc attaaggtct tttggcatta tcttgatacg
     2641 ctctccaaga taccattctc caaatttcgg tgtttttttc cccagttttt ctatttccgt
     2701 ctcatcgata gaaaaagttt ctttttttgt tttcttttca gacttcttat caatcaaatc
     2761 agtgcttgcc aagatttgtg ttatcattgc tgttgtaagt aagagtattc tcatcatttt
     2821 attaaaattt tcctttcaaa gtttgtctaa aatttcactt aaaactactc acaagactgc
     2881 tgcacaaaag ataccatcca tccacaagca caaagaaaat tactttgaag gggagtgata
     2941 tcatcacagg gggcaacatc atcataccca tcgccatgag aatagaggcc acgacaaggt
     3001 caatgacaag aaaggggaga aagattaaaa aaccaatctc gaaagcacga cggagctcgc
     3061 taatgataaa agctggaaca acgattttat aaggaagaga ttccccctca tgaggcggct
     3121 caattttagc aatatccata aaaagtttga ggtctttctc gcgaatgttt ttaatcataa
     3181 aatcacgcag cggcacaaaa gctttatcaa acgctttggc ctcgtcaatc ttttcctcaa
     3241 taagtggctt aataccttcg tcgtaagctt tctccagtgt cggcgccatg ataaagatcg
     3301 ttaggaaaag cgcaaggctt gctaaaacaa cgttgggagg cgactgctga agcccaatag
     3361 cgttgcgtaa aaaggaaaac acaatcacga tcctggtaaa agaggtcacc atgatgacaa
     3421 tagagggcgc taaacttaga acggtaaacg cgatgaggag ttgaataatc cgccctgtca
     3481 tgcctgaaga attcccaaga tcaagggtta aggattctgc aaaaacaggt gttgttgcac
     3541 ccaaagcaac cataaaaaga aaaaagaata aaaggaaaaa tctacgcacg tttgctatcg
     3601 agtgtcacaa tcgaggcaaa ctttttctcc tcgctgtgcc cttccaggct atgattttct
     3661 aaggctgcga agtgatcatt atgagggccc acaatataga tatatttttt gccacaaaga
     3721 ctcatgatca tggcgcgacg ctttgtgtca atcaaaagac tttcctcgat ttttaatagc
     3781 tcttgtttgt gaaatttgtg ctgcatacgt tttaaaagaa cagaccctaa aatcataacc
     3841 gcgacaaggg cgatcaaaat acacaaagcg ttgacataat cgacaaacat tattttttcc
     3901 taaaacgatt ataagcactg gcggct
//
LOCUS       DAIAHG010000101         3898 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_101_length_3898_cov_4.505103, whole genome shotgun sequence.
ACCESSION   DAIAHG010000101 DAIAHG010000000
VERSION     DAIAHG010000101.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101_length_3898_cov_4.505103"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1161 a          857 c          784 g         1096 t
ORIGIN      
        1 cctgtcggcg ttgacaccct cgccgctgca ctttttgagc aacgtgacac actcgaggaa
       61 gtgattgagc cttatcttat tcagcaagga cttgttcagc gcacgccccg cggacgcgtc
      121 ctgagcgaag cggcgtatga gcacctcgaa gattctttta tggacgatta attttcctcc
      181 ctaaaatatt ttaattagag attagagaga atctgacaag aaacttatcc aaccttttcc
      241 cacaaaggat ttttcagcgt ttgaacaaag gctttgtgct ttaaaagctc ctcctctgac
      301 atttgaaaat gacgcatagg cttaagtgca cgctctttca tcatagatac agaatgtttt
      361 tgcttatctg cacgcaaaag atccagatcc ggttgacggc cgccggataa ttgcagataa
      421 acttcggcca aaagcatagc atccaaaaga gccccatgtt ttgtacgcgc agaaagatca
      481 atctcaaagc gcctgcacag cgcatcaaga ttcgcaggcg accctggaaa aagagtacgc
      541 gccattttca ctgtatcaat ggcacgggtc ataggcagcg ggtttagccc aatcttgcca
      601 agctcgccat taataaagcc catatcaaac ggcgcattgt gaatcacaag aggcgaatcg
      661 cctaagaact ctaaaaaagg ccccacaatt tgatgaaact caggtttacc gaacaacatc
      721 tcgtctgtaa taccgtgaac ctgtgtcgct cctggatcga taagcctccc tgggtaaaga
      781 taggtgtgaa aagtctctcc cgtgggcata tagtttttaa tttcaacaca cccaatttcg
      841 ataatacggt gccccgcggc aaagctaagg cctgttgttt cagtatcaag aacaatctga
      901 cgcacgcaca gccccaagta tgtgaaatta tctctttctt tttttaaagt ctttcaaaag
      961 aaaagtcatg ttttttctca ctgattttta gtgcaacgat gaaatgtttt agttatttat
     1021 ctcgccctcg tgactgggct ggttatgtgt cggccatggg tgatgaagat caccaagatg
     1081 gcatagaagg gaagcgacct caagacggat ctcgctttgc cctgaaaaaa taagatgcac
     1141 atgcgcatat ctccccttct cattgctttg gattgttagc aagttcagcg gatgcttctc
     1201 aaggcttcgg tgaaatccct tgtgatgaac agacctgacg tgacgaaagg taagccccgc
     1261 atgcacacga tgataaaggg ctttgttctg aaaaaaatga ccatcgtgtt cccaacaaaa
     1321 acggtgacaa agaccactaa atgtttgctg ttcaggatca aaatgaagac tgtgcagcgg
     1381 gaccaaggca tcttgcagat gggccgagag aatatgaaga tcttctgtat cctcggcctt
     1441 tatttttaac cgcggcaacg ccatactgtc caccaatgtc atgttttttt aatctccact
     1501 tacagcaata cgctcgatat gagcaccaca attcctaagt ttatcttcca ctgtttcata
     1561 accacgatca agatgataaa tccgatcaat cattgtttct ccctgtgcag caagccctgc
     1621 catcacaaga gaaatagacg cccgaatatc tgtggccatc acaggtgctc cttttaaagg
     1681 tgtcccacaa cgcacaagag cactggaacc gtgaaccgta atattcgctc ccatacgaca
     1741 aagctctggg acatgcatga atcgattttc aaaaatcgtt tctgtgatca tcgatgcacc
     1801 cgttgcaagc gtcataagcg ccataaattg agcctgaaga tccgttgaaa aacctggaaa
     1861 aggctctgtc ataatatcaa tccctttaag attccccgcc tttgctttaa acccattctc
     1921 tgttggtgta aaaatcatac ccgcttgttc taaaacactc acaaccgtgg gaataagatc
     1981 aagggacgtt tcgttgagag tcacctcgcc ccttgtgatt gcggcagcca tcgcatatgt
     2041 ccctgtttca atacgatcag gttgcacagt atgggtcgcc ccatgcaatg tatccacacc
     2101 ttcaataata atggtatctg ttcccgcatg ttcaacccga gcccccatcg cattcaaaca
     2161 cttggctaaa tcgataattt ctggctctct tgccgcattg ataatacgcg tcgtcccttt
     2221 cgctagaaca gcggctaaca taacattttc cgtcgccccc acagagatca tcggcatact
     2281 aatatccgca cctctaaggc cattactcgg tgctgttgca tggacataac cactctcgag
     2341 ttgaatcgaa gcgcctagtt tctcaagccc catcaaatga agatccacag gacgtgtgcc
     2401 aatcgcacat ccccctggca gtgatacaat ccctgaaccg tgacgcccaa gaagagggcc
     2461 caaaacgaga aatgacgcac gcatacggcg tacaatctca taaggcgctg tgttgctttt
     2521 aagtgtatgc gtacacaaag taagatcagg cagattgtcg ttaatactta cgccatgctg
     2581 atccagtaat tctaataatg tgcgaatatc cgcaagattc ggtacatttt taaagatcat
     2641 aggctgatcc gtcaaaaggc tcacgcaaag aagaggcagc gccgcattct tagccccgct
     2701 aattgtgata tctccagcaa gaggttgtcc ccctaaaatt ctaattttat gcatgttttt
     2761 gataagaaaa atatgtgaat gattgcccct agataagcac attttaatca acgaggaaaa
     2821 gagctcatta taaagactca ctaaaaatgt ttgaaattaa aaaatcgaga tcatcatcac
     2881 gactcctttt gataaaatgc ttaaaataat catgaatctt gatcaatgca gcagttaata
     2941 cagatccttg aaattgaaaa cccaacggaa tcagatattg aaaaagatgc aaatctcaca
     3001 gtaccgccac attacaaatc gcgtgttaat ttagacagta tgccgcgact aaatttacaa
     3061 gcgccccttc tttaaaaaat tctttaacat atatcccatt ttagtgattt ttgacaaaaa
     3121 ttctcgataa tttcgcgtgc cttcagatag aatttaggaa aaaatgggga aaatgaagtg
     3181 taaatacaag aaattttcat cgtctacttt ttgaagtgct ttgatttagg aatgcacacg
     3241 atgatttttc aaaaaagtgt ccttaggata gcatagcaat gcgtggaata cagcccattt
     3301 aaagtgtatt caaagcaagg aaagcctgat tttgatgatt gctatgaaat ttctcaatct
     3361 ttttcattgg aaaaaacaaa aattgggcca taaaaagctc aattaaggca taaatagcgt
     3421 gatttcatgc tttttttctc tcagaaatgg gttttcacaa cacatttggg gaaatttcga
     3481 gggtatttca tgaaaaatag tgtatttttt gctaaaaaaa ggggtttttc agtgaaaaaa
     3541 aagattttga ggtgaatttt cccttttttg accgagattt tgtggttttt caggcaaaaa
     3601 agggctattt taacgcatta ggcacgaaaa taggtgaatt tttaggattt tgaggcgaat
     3661 ttttcacaaa aataattgtg atggagggag ctgaattcaa gggttcttgt ctccttaagg
     3721 ggtggtggta cggagattgt acgcgttttt tagaaaaatt cttctactaa tgcagtgatt
     3781 ttgtgtgttt ttcatgtaaa aaaggctttt tgacccagta ttttgaaaat ttcgataaga
     3841 tccatcgtat tttttgcgtg actaggcgat tttttaggat ttttgaggtg aattttcg
//
LOCUS       DAIAHG010000102         3898 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_102_length_3898_cov_2.601937, whole genome shotgun sequence.
ACCESSION   DAIAHG010000102 DAIAHG010000000
VERSION     DAIAHG010000102.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102_length_3898_cov_2.601937"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1400 a          767 c          635 g         1096 t
ORIGIN      
        1 aaaatcttta aaaaactttt ttaggagtgc cgttcaaaaa tgtgctagga tgaagaccat
       61 aatttctcgt tgagatacat ctcatcttat gaaaaataag acctgtagcc tttgtcatag
      121 aacctttgcg gaacatagcc tttttcctat tgaccttctg aagcccggca ttcaagagct
      181 tatcgcccaa catcatgtct tctcctctaa atcctttatt tgccctgaag atttaacaga
      241 attttataaa aaatggcgca agcaacaaat tctcgaaagc gctcatatat gcaatctcag
      301 tgaaattggc ctcaaatctt ttgacgaaag cctcctcaat gcaaaatctt ttgatgaact
      361 tgaggacggc cgcctcacca tgggcgaacg cctttctgac cacctcacct cctttggcgg
      421 cagctgggcg tttattctca gtttttttat ttttcttttt ctttggatgg catggaacag
      481 ttatctatgg atcacagacg ccttcgatcc ttatccatac attcttctca accttgtctt
      541 gtcttgcctt gcagccattc aggcccctgt gattttaatg agccaaaaac gccaagaagc
      601 cagagatcgt ctccgtgcag aactcgacta tcacatcaat cttcaagcag gcttagaaat
      661 ccgtgacatc agcgcaaagg ttgatcatat cggtaaaacg ctctgggaac acctcttaga
      721 gattgagaaa accctggaaa aacgctctga taaaaaaact ttagaaaaat aaattcccct
      781 tcaatgcctt aaaacattaa aaagcagctt aaaatatata gctcttgaga taaaaatact
      841 ttatctttca ttaactattt ttatgctttt taatgtttta agaatcttac aatgaagtct
      901 tgtctattta aaatactttt actttgcacc attttctcat cggcacatac cagcgaaaat
      961 aaagaaataa gtgaaaacaa agagatacat gatatcccca aagaaatttc tcaaaagaag
     1021 aaaaagaaaa aagatattga attcccaggc aaaaattcaa cctattgtac ggatgcaaag
     1081 aatcaactcg gcaaacctat aaaaaatgat gagaaggggt ttaaatttgt cgaaaaaata
     1141 aaaaagcaac gcaaagccat gcaaaaaagt ttatttgaaa aaatttttgg caaacatgat
     1201 gtcattgatt ttttttatgg tgaccgtcaa aacgctttta atgatccttt ttattttcac
     1261 caaacatctt tgcaagaaaa tttatttaaa ggacataact gcaatgataa cagcatagag
     1321 agctttattt tccgccccac tgaattcagc acaatctcaa aatggatgaa tgaaaaaatc
     1381 attaaccctt acaaagatcg cacagttcat ccactgccct atggtgacga gcgcatgatg
     1441 ttttggaaaa acattgaaac cctaaaaacg ctttttgcac acttttacac ctacctcttt
     1501 aaaaaaacag agctcgacta tcccacacaa ggcccaagga tgcttaaaat ttgggaaaaa
     1561 ataaccgaag agaaatcaaa agaagagctt attgaagaga tgtctagcct ctttattcaa
     1621 gcacacggtc tttcttttaa taattttttg aatacaaaag agctcatttt caaacactac
     1681 cttccagaaa acaaagaata caatattaat atcttagccc cttttagctt ttttaatttt
     1741 ggatttatgc ctgaccaact tcactgctcc ttagaaaaat ttaactattt gggaaaagaa
     1801 gatgtggtca ctttttttct gcatgcccaa atgaaagttt atcttcaaaa atctctcagc
     1861 ggccttatcc atgaatatgc aaaagatctc gccacgatct atagtcacta cgacacttat
     1921 ggaaatctta atgaacctta cggaaaaata tttggaaaga ccccggattg ctttaagtct
     1981 catttatgct attcagttgt agaacctctc agtgagaaag atcttcaaaa actcgaaaaa
     2041 tccctaaaaa gcattaaagg atttgatgat cttgttattc caaagacaaa agaggaagag
     2101 attaaagacg aaaaaataaa caaagagctg gaggaaattt caaaaaagga gcaggaaaag
     2161 ttccttaaag agcttgaaga aaaaaataaa aaaaagaaaa aaaatactat cgacgagaaa
     2221 aaaaagattg gtgtaaaaaa ttcttcaccc aaaaatatta taaaagaaga aatcctcaaa
     2281 aagatagaag ttaaaaaaga aatagacatt agcgagatta agaaagaaat tgttctcaaa
     2341 aaagaaaaaa tcgttaagaa aaatttttct gaacttcaaa aggaaaagtt tgacgatcta
     2401 aagaaaaatt tctcgaatac aaaagaatac tcacgcaatt ttttaacctt tatcaaagag
     2461 tttgccgggg aatttaataa aaaaacaccc ctaaaaatca aaggtcaaaa tggatgggat
     2521 gccgcaccag aagatgttat cacccttgta gcgggcgggt cttttgctct gaacatggca
     2581 acgccaaagt cagatttcga atattttgtc cttgtaaagg aaaaagacct caaaccggaa
     2641 atttttgagt ggctctctgg ttttaacaaa acccttaagg acatagggat catttttgat
     2701 agtgaaaaca actatccccc tattttgaat aaaaaaggcc aaactctcgg tgggtctcct
     2761 tttaccatag caacccctga gatgtttgtt tcctacattc atatgatttt ggagatggat
     2821 ccccgcgcaa aaattgaaaa aggaactttc ttccaaaatt tctgcaaaaa attacccaaa
     2881 aatgtcaaaa aaatttatga gagtgttagc acacccacta aggaaaaatt ctcagagaat
     2941 ggtgtttttt atgatgaaaa cgtctcaggg aaccttataa aaaatttgta tacgatagcc
     3001 ctcacacatc aaagaatttt agggccaaat aaaaatctga cgcgtttaaa tgagcttttc
     3061 tcgaaaaaac acaaggaaga gattcattac ctcaacaaca ctatctccct acaagaatac
     3121 atctctcagt ttttaaaata tggttatgcc aaaaacagta agctcaatat ttttttcaat
     3181 gaaaattttt ctttagaaaa aatagatgta aaatatcacc tgtatcgccc ccttcatatc
     3241 tatctccagt atcttgcgct taatggtaaa gaggtggatg aggaaattat tacctttaag
     3301 gagtatatcg ccttgctcag gggtcttaaa actcaaggag aactcatctg gacagaagaa
     3361 tctcttaata tttttaatga aaaatacaaa aaaacactcc tacgtattaa taaggagctc
     3421 acatcgaaaa attagagaag aggtggtgcc cagagccgga atcgaaccag cgacacaggg
     3481 attttcagtc ccttgctcta ccgactgagc tatctgggcg tgcatgcaca ttgtatagcg
     3541 gattttattt tctctgtcta tggaattatc aaaaaatacg tcccctatgt ttcagtcgca
     3601 atctgcacct gtgacccaga tgcaacatta gagagataag ggatatcaaa aaagtattta
     3661 cgattgtttt tagcaacgct ctcatgcatt tttattttga atccacctaa aaatgtataa
     3721 ggaaaaaaat ggtcaacacc attacaaaac tcatgctttt atccgcagca ttcgctggat
     3781 tagcatcccc cattttagcc gctcaagcgc cagcatcagc gtccaaaaca accgcggcac
     3841 ctgctgcttc tggaacgact acatcttcta ctgaaggtca gcaaacaact gaagcctc
//
LOCUS       DAIAHG010000103         3897 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_103_length_3897_cov_3.044503, whole genome shotgun sequence.
ACCESSION   DAIAHG010000103 DAIAHG010000000
VERSION     DAIAHG010000103.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103_length_3897_cov_3.044503"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1188 a          672 c          860 g         1177 t
ORIGIN      
        1 tgaaaatctt ttgaagcaga aaaattctat tctttcttcg ggggcggctt gtgatatttt
       61 tggaacgctt catcaaacac ttttagattt tgaaaaaaac tacattactc ctctcacaag
      121 cgaaacagga gatgatctta aaaatcatgt taatgagacc ttgaaatata tgaatggatt
      181 cacgcttagc acagaggaag ataagaagtt ttccgtaaaa ccttttaatt caaagggtat
      241 taatcctctt ttcatagggc cacatctttt ccttccaagt gaacttgttt taaaatcact
      301 taaaagaaca gctcttgatt ttagctacta tacatctgtg acgacggatg ataatgacaa
      361 caccataaca gctacagttt ggagctataa aaacggtcat gatctcaacc aatccgttga
      421 aattgatgat aattcgacag atattacatt tccaggaacg aaaaatcgtc ctgatggatc
      481 aatgcttatc cttatcaaca atgaaaaagt taaagatcct ttttctaagc gagccaatga
      541 caatgcttcg gaaggagctc aggtcaatgc ctctaggctg ggcaatgcaa gtgtaaaata
      601 tggtagctgg gaagtaaaag tgccagatac gatttatgca acatttgcgg cgcttcagac
      661 aagctcctcg cttcttcaag aaggtgattt taaagcgatt caaggttatc ttaatgcaac
      721 tgttaacaaa gtgttaactg atcttgtgac tattcaaaat gaaattgaaa aactatcaac
      781 gaattaatgc gttaattttt ttaattttta atgaaccccg tcagcttggc ggggtttttt
      841 ttgtgcacaa gagtgcgcat aagtgttatc aaatcctgaa aattaggata cattacgtaa
      901 attagaaaaa tttttttgta atttatcaaa acaaagggag taagtgcact atgtggacat
      961 caaaagagat tgaacaagct ctgaatataa gtgggattag tctttctcag ccatgtacag
     1021 ggatttcgat tgatagccgc acagttaaaa aaggtgatct tttctttgcg cttagaggtg
     1081 aaaatagtga tggtcatttt tatgtgagtg aggctcttca caaaggagcc atagctgttt
     1141 ttgttgaaaa aatccaagat cacattcccg cagcaaaaca aattcttgtt ccctccgcgc
     1201 gaaaagcaat ggagcaattg gctgcttttg cacgccaaaa aacagaggca aaggttgctg
     1261 ctattacagg aagtgctgga aaaacgacaa cgaaagaaat gcttgcgttc attttgggca
     1321 aagtaggaaa agtgatatac tcacgggaga gttacaataa tgatattggt gtgcctttca
     1381 gcttagctca cattgaaaaa gatactgact ttggtgtttt tgaggttggc atgaataatc
     1441 ccggcgagat tgcgccatta acgatgatgg taaggcccca tgttgcgata attacgagtc
     1501 taggggaatc tcatatcggt ttaatgggat ccgaggaggc gattgctgag gagaaatctg
     1561 aggtgatgcg ggggcttgac tcaaatggta cagcgatttt tccccatgac acaaagcata
     1621 cctctactct ccttaaaaaa gcacgttctt ttagtattga acatattaaa acttttggca
     1681 aacaagaggg ggcttatgcg caacttttaa cgtttaagcc caataatcag ggaacagggg
     1741 gcgaagttac cgttaaaata ggtgagaaaa cctacgtata tattttaggc ataccgggag
     1801 aacattctgc tcttaactcc ttagcggtac ttcttgcggt tgaatctttt ggtctatcga
     1861 tcgaggatgc gctgccgttc tttaaggatt tttcgcctgt tcgaggacgt gggttgaggc
     1921 atgtgttgcc ttttaaaaat gggtatttaa cattgatcga tgacgcttac aatgcgaatc
     1981 catcctcgat gcgtgcgggg ctttctgttt taggcgcaat tgattcgatt gataaaaagg
     2041 ggcgtaaaat agctgttctt ggcgatatga aggagttagg gcaggggagt ccagaatatc
     2101 acaaagccct ttctcttgtg atcgatagtg ttggtgtcga ccttgtgttt gcgtcaggga
     2161 cggatatgaa atatctttat gatgtgctgc ctcttgcgaa aaaaggtggc tattcagaaa
     2221 cacccgaggg attaatccct attgtcatgc atgctgttca tgagaatgat cttgtttttg
     2281 tcaaaggatc caaggggagc tatgtatcga aagttgtgga tgcttttttg cataaaaaag
     2341 tggggtagtt tcaaagaaaa agtaatgata aattgatttt ttctgctctt ttttagataa
     2401 taatttcaag gaatgggcat aattttcttg cctcttgagg ttgcaaacgt ttaacctggg
     2461 tggaaaaaat aaatcatgaa agattctctt caatgacaga aacacttgct tttgaggtgt
     2521 cgcagaaaga gcaaactctc tcacctcaag ccttaacaga gatgcttgcg aaagttttag
     2581 atgagaaaaa agcacaaaat gtaatcactc tcgatattcg cggaaaggca agtttttgtg
     2641 attttcttgt gattgcatcg ggaagtgtct ctcgtcaggt tgtggcgctt ggcgatcatt
     2701 tgctgcgcgt gcttaaaaac cagggtattt atgcaaccat tgagggagag gaacaagggg
     2761 attgggtttt gattgatgcc ggagatgttg ttgttcatat ttttcgtccc gaggttcgag
     2821 agcgttataa ccttgagaaa atgtgggcag cctaaacctt gcaccttcct tttaaaataa
     2881 ttgctgtggg ccagcttaaa agcggtcctg agtatgatct ttttcagcaa tataaaaaac
     2941 gccttagcca tgttgagatt atccagctga aacaatcgac caaggaagca gaagaaaaat
     3001 ttttcctcaa gcacataagg gaatctgact gtatcatagg tttagatgaa caggggaaag
     3061 actatgcaag tattcccttt gcgcaatttt tggcagcgca attaggaacc tataaaaata
     3121 tttgttttct catcggcggc gcagacggct taagtgatgg tgtgcgccag cgttgtcacc
     3181 atcttttatc ttttggaaag ctcacgtggc ctcatatgct tgtgcgtggt atgttgatgg
     3241 agcaaatgta ccgtgcaacg cttattctta acgggcatcc ttatcaccgg gaataatttt
     3301 taatcaatta ttgtttgtgc taacaatgag cttttaatat gtaatgtaag gaaaatagta
     3361 tgtcaaaact aaagaaactt atcatcgctc tcactttgtg tatcggcgca acgcaggtcg
     3421 ttgcaagtga gggtgagaaa attgtcatta atgaaaaaga ctataaaacg ttgcttcctg
     3481 ccattgtttt tctgactggt aaagataatg aaaattatat cgaagtaagc aaaaatttaa
     3541 tgaaaatcgc tggactattg cagactatat tcttggaaaa aacaacaaag aggtcaaggt
     3601 tccaggttgt cttcaatata tagctactca aacagaaaag gaattgagtg atttttccat
     3661 aaaaaagacg cgtatttcaa gtgatcgtat ttcagatgat atatctatca aggtatttgt
     3721 acaatacagt ggcaacgatt ttgatgatat taggactatt ttgaataaaa actagttttt
     3781 gaagagaggg gggctgtatt cccccttttt ttattagtta tcaatacccg catcagcaag
     3841 ctttgccgct tggtcttctg agataagaac atcaatcact tcgtctaatg cctcacc
//
LOCUS       DAIAHG010000104         3889 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_104_length_3889_cov_4.311910, whole genome shotgun sequence.
ACCESSION   DAIAHG010000104 DAIAHG010000000
VERSION     DAIAHG010000104.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104_length_3889_cov_4.311910"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1166 a          860 c          778 g         1085 t
ORIGIN      
        1 cccactccca catctacgac taagggcaca gaaaggttta taatcgtttc catactcttt
       61 ttgataaggg ccgtgatcgc ctccacagag tcattatgaa tctcaaaaac aagctcatca
      121 tgaacttgca gtaacatacg ggcgttaggg gaattatgcg ccaaaagtgc gtcaatttta
      181 atcattgcac atttcataat atctgcattg cttccttgca gcggtgcatt aatcgcctgg
      241 cgctctgaga aattgcgcat agcatagctt ttatcgagaa tccctggtgt aaagcatttg
      301 cgtcccaaaa gcgtttgcac atacccatgg ctgcgcgcga aatctttcat accctccata
      361 tacgcttgaa tgccagggta ctgtttaaaa taaagattaa tataattttg cgcctcagcc
      421 tgaggcactt taagctgctg agagaggcca taggcgctta tgccataaat aataccaaaa
      481 ttaatcgctt ttgccgaacg gcgctgttcg tctgtgacct gatttttcgg tatattcaaa
      541 atctgactcg ccgtgagcct atgaatatca accccctcgc aaaaagcctc cttgagggaa
      601 ggaatatccg ccatatgcgc caaaagacga agctcaattt gcgaataatc aaaactgaca
      661 agtttaaaat ctttttccgc tataaaggct gagcgaattt tacgaccatc ttctgtccga
      721 ataggaatat tttgcaaatt cggatcagag gacgataacc gccctgttgt tgttcccgct
      781 agattatacg atgtgtgcac acgctttgtg tgaggatcaa tggctgcaag aagcccctca
      841 acataagtac tttgaagttt gacgaggctc cgccaatcca ttaatttttg cgcaatggga
      901 atacccgctg tgacaagatc ctcaagcaca tctgcatgcg ttgtaaacgc ccccgttttt
      961 gtttttttag gttcaggcaa cttgagttct tcgaataaca cctgagaaag ttgtttgggt
     1021 gaactaatat taaaagaacg tcccgcaagt gcataaattt cttgctctaa aagacttgcg
     1081 cgttttttaa aatcctcccc gaggttttct aaaatcgctg catccacacg aatgccacgc
     1141 gcttccattc ttagtaaaat aggtacgaac ggccgctcga ttgtaaaata aagatgcgcc
     1201 attttttctt tatagagacg cacacggagt aactggtaaa gacgataggt atagtcagca
     1261 tcctctgcag catattgcgt cgctttggca ataggcacat cggcgaaatt aagttgcttt
     1321 tttccagagc ctgtaacatc ggcataggca atcattgtgt gattaaaata atgggatgcg
     1381 agaaaatcga gaccatgccg atgtttacct ccatcaaggt catacgacat cacaagtgtg
     1441 tcatcaaaga cgtcaaaggc catagcgtat ttttctaaaa tcgcaagatc gtatttaaga
     1501 ttatgaccta tttttaaaac actaggatcc tgcaaatagg gggttaagac cgcgtgcaca
     1561 tcttctattt taagctgcat tccgccattc aaaagacccc gatgctgaag aggaatataa
     1621 cacgcatttc cttctccctt tgcaagggct atgccgacaa gctctgctgc ttgaatgttt
     1681 agtgatgttg tctcacaatc aatggcaaga tgggttttaa catccccaag ccaatccata
     1741 agagcttctt ttgttgtaat cgctggatag tcagcttgtt cttttgtagc tttttcaggc
     1801 ccactcatac ggttcaaaag actagtaaaa ttttgttttt tgaaaaacgc tgcaatactt
     1861 tctttgggat aatcatgatg atgaaaatga tcaatcgcaa gatcaagggg aacatctttg
     1921 cacaaggtca caagtttttt cgagagaaaa gctttgtctt tgttctcctc gagagtttgc
     1981 cgacgtttag gttgcttaat ctgatcgagg ttttgataaa gattttctaa ggatccaaac
     2041 tgtccaataa gctctgctgc tgttttaggc cctatgcttg gcacacccgg aacatgatca
     2101 ctgctatcgc caattaaagc ttgaacatca atcactttct caggatgcac accaaatttt
     2161 tcaaaaactt cgtcaaacgc tattttcttg tttttaaggg gatcaatcat tgtcacatga
     2221 tcatcaatca gctgcattaa atctttatcc gccgaaacaa taacaacctc gcaccctttt
     2281 gtcttcgctt gctctgcata ggttgcaatc acatcatccg cttcaaatcc aactttttcg
     2341 acaacaggca gattaaaagc aagacatgcc tcgcggaaaa gaggaaactg cggaatgaga
     2401 tcttccggtg ccggcggacg atttgcttta taatccgcgt aaagtgtatg gcgaaaagtt
     2461 tcgcgcccag catcaaacac aacgacaaca cattttgaaa gctctggatt cgcgagaaac
     2521 ttaagcagca tactgcaaaa tccaaacaca gcccccacag gtgtgccatc tggacgcgta
     2581 aaagggggca gtgcgtggta agctctaaaa ataaaacctg atccatcaat cagtgtgact
     2641 gtttttttca taaaataatg tcatgactca acgtatgtga tggagggtac aagatctttc
     2701 cctattttaa aagccctctc gcacaacgtg ttaccttgac atttttgcgc gatatcctac
     2761 gatggtggcg gtagaacact atctttttaa ggaaccccat tttatgaaaa acatattcct
     2821 cctcacctcc cttttgtaca catgtttggc aactgttcag gctgcggata aggatgaaat
     2881 tttatcgaag gaatctatag ataaatccat ggccacaacc tttataaaaa taggtactga
     2941 tttgccaagt atcgctcaaa ttgacaaaag gtatagtcaa gcaatcggag tctgtgatcc
     3001 aaaaggtttc gcgcgcaata ttttgataat ctacacgaaa aaaggaaaag aatgttggaa
     3061 tttaacaata ttgccagacg ataaaaatat aaaggtgagt gcctggtcag aggatattac
     3121 ttttgaaaag gggattccca catcaaaaac tcttccaaaa attatgatca cccctgaaaa
     3181 tgataaaaaa tcgtttgata ttaagctaga tatttttaat ggagaagaag gactcacaac
     3241 gctgagcggt tgtgaacatc ttgtctgcag aacgttcgac atggatggaa atcaaaaagc
     3301 aaatgaggat tataatgaat ttcaattaga ttatcttgcg tattatgcgc gcaaaaaaga
     3361 taataacatt taatcgcccg cctcaacgat atgtaaaaaa aaagctcccg tttaagggag
     3421 cctttctcat ttactttttt gcacaaaaat ttatgtgttt ttagcaataa tatcatcagc
     3481 aacatgctgt ggcacttgct cataatgatc aaaatgcatg gaaaattgtg cacgcccttg
     3541 gctcatggag cgcaatgtat tgacatagcc aaacatagaa gcaagaggca ccatcgcatt
     3601 cacgacacgt gcgttaccac gttgatccat acctgtgact tgaccacgac gactgttgag
     3661 gtcaccaatc acatcgccca tataatcctc gggcgtgaca acttcgacgc gcatcacagg
     3721 ctcaagaatc tttggtgtac atttgcgaat accttcacgg aacgctgcac gcgctgcaat
     3781 ttcgaaagca agggtgctag agtcaacgtc atgataggca ccatcaacga ggcgtgcctt
     3841 gaaatcgatc atggggaatc ctgcaagcac acctgtatcc atcgcagag
//
LOCUS       DAIAHG010000105         3883 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_105_length_3883_cov_3.340778, whole genome shotgun sequence.
ACCESSION   DAIAHG010000105 DAIAHG010000000
VERSION     DAIAHG010000105.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105_length_3883_cov_3.340778"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1177 a          803 c          736 g         1167 t
ORIGIN      
        1 gggcaggact atgcccgcgt tatccctttt tggagagaaa tttcccaacg ctaccggtga
       61 ttcatcccgc gcatcatcac aaagagctct ccgtcttgca tttaatcatt gaacattaat
      121 attatatgtt tcataaaaaa aatagacttt aaatgcaata ttaagacgaa aaatattatg
      181 aaattaaata aattaatcac ttttaccaca gcctttatgc tactaagtgg cataagtgca
      241 ccttttgcag ctgatccttg taacaaagag gatactaaat catttgttcc aaaaacctat
      301 gaaaacactg tcgaaatcaa gaccaataaa ggagaatcat ccattatgaa caaactttct
      361 aaaaagtttg aggagattgt tttggagatg gaaaatattt cagatgagaa aaaacgtagg
      421 gagtgtcaaa gcaaactact tcattctttt gtagaactta cattttttca ggattttttg
      481 gaaaaaaaag actaattgtg atttccgtaa tatcataaac agtatttcta tctcaaattt
      541 cattgagttt ctagaggaac ataaatatga aattgatgga ctccaattat gggatgtgtt
      601 aatacccaat tattcagagc aagctaaaaa gaaaaatgat catcatgaaa aactcaatac
      661 ggccgtgctt gattttttaa caaaaaccaa agtaagccgt tttgaatgga caattcatca
      721 tgaggctatc gaccttttca agtctatcaa aactcccgaa gttatcttta atgtttataa
      781 gatgtctccc tggcccactc ttgtaaattt agcaaaaaac cctaatgtca aaaaaataac
      841 tcttgttcct tttctaaatc agcctgatta catctatatc aagaatagca aaaaagaatt
      901 tcttgacgcc ctccaatgca ataaaaccct cgactatttt cacataaagt atgacagtgg
      961 cagtatgcgg ttaaaagatt gcggaaaagt ggtagaaaaa tattacaaag gcatcaacca
     1021 tcaaattaat gatattctta gcaaaaataa acaactaaga cataagaatt acgattttaa
     1081 tttttttata agtaaatagg tggcttttgc cacttatttt tcacctacaa agaaaaatgt
     1141 tgtcggcaaa gcgcaacata gcgatcgttg ccgccaattt cgacctgagc cccttcttta
     1201 atccgcacac cgttttcatc cacccgagcc gtcatcgtcg ctttgcgccc acaatggcaa
     1261 atggttttta tttcaatgag ctcttccgcc aacgctaaaa gcgcatgact tccctcaaaa
     1321 gtgttgccta aaaaatctgt acgaaggcca taagcaagca caggaaggtg gagatgatcc
     1381 accacctgac acagttgttg cacttgtgtt tttgttaaaa actgcgcttc atccacaagg
     1441 atacacgcca gtttcgagag aacttgtttt tggtgaccga tctccttaaa aaaatcaaaa
     1501 gagcgatcaa acgcaacggc ctcactcgca agaccaatgc gcgatgccac acggccactt
     1561 ccgtaacgcg tatccaaagc gggcgtataa agaagtgtct ccataccccg ctcccggtag
     1621 ttataacttg attgcaaaag cgttgtcgtt ttacccgcgt tcattgcgga gtaataaaaa
     1681 aagagttttg ccataagttt tccctactgt gtgttatctg ttattcagca aaaagtttgc
     1741 tccaaaacca gcttccccag ccaatatttt cattgacttc agtctgcttt ttcttaccat
     1801 cctcaggttc gtcatcgcca tcgcacgtaa actgaacaat ttttttatca tttacttttt
     1861 catcaaattt atttgtatca agctctttat ccaactctct cttgcttttt acactcacga
     1921 tggacttttt gccatcctta ctatgaatga tttttatgtt tttcatgtca cagttattat
     1981 ccaataacat ttcattaatt tggtttttta aggcttcagt tgcatcatgc tctacaaaat
     2041 tacgattaaa ttcattattg cagattcctg tgttacttaa ggaggaacag gatttttcat
     2101 cccgaaacaa gccaatggat acttttttat tacttttcct gtaattttta tgaggattta
     2161 ctgcattact cacgtactcc tcctcgtgct taagaaaagc aatagaatct ctcgtagtcg
     2221 tgcaataaac attttcattt tccattttta catcactaat ccatggcgca ccacctttat
     2281 agaagcactg tttatacact agtttacctg caccggtcgc caaatgttct accgttcccg
     2341 ccattcctgt ggacaaagtc gcgataagaa agactgcact gcttatgatt atttttttga
     2401 tgatcatttt tattttcctt tgagaaattg ttttttttga atcaacattg tatacataca
     2461 gataaagaat agtgattgca aaaagaattt taagatgatt tctctccaat ttcccgatgg
     2521 tgcaaaacgt gattatgcgc cgggtataac aggctttgag cttgctgaag gtatttctaa
     2581 atcccttgcc aaaaaggctg tggcgatgaa agtaaatggt gaactgtatg acttgacgcg
     2641 ccctttaacg acggatgcag cgattgaaat tattttaaaa gactcccccg atgcccttga
     2701 gattttgcgc catgatgccg cccacgtttt tgcagaagcg attaaggagc tttatccaga
     2761 cacacaaatc accattggcc cggcgattga aagtggtttt tactatgata ttttccctaa
     2821 aacaccgcta acgccggatg atcttccaaa aattgaggcc cgtatgcgtg agatcgttgc
     2881 acgcagcgag ggaattatgc gcgaagaatg ggaccgagat gaggccattg cgttttttaa
     2941 aaagcaagga gaacatttta aagcagagat tattagtgac cttcccgagg gcgaaatcat
     3001 tagcctttat cgtcagggca attttattga tctgtgccgt ggtccccata tgccaaacac
     3061 aagttatctt gggacagcgt ttaagctttt gagtcttgcg ggcgcgtatt ggcggggcga
     3121 ttctaaaaat caaatgctcc aacgtattta tggcacagct ttttggactc aggctgatct
     3181 tgatctctat ctttttcaga tcgaggaagc taaaaagcgt gatcaccgaa agcttggcgt
     3241 agagcttgaa ctttttcacc aacaagaaga ggctgcgggc gctgtgtttt ggcatcccaa
     3301 aggatggaca ttctatcgca cgttagaaaa ctatattcgt gtaaaattag aacaaaatgg
     3361 ctacgtcgag gtaaaaacgc ctcaacttgt ggacagatct ctttgggaag cctcagggca
     3421 ctgggaaaaa tttcgccaaa gcatgttcac agtagaggcg gatgaggata aaactcttgc
     3481 ggtcaaaccc atgaactgtc cgtgtcatgt gcaaattttc aagcagggcc ttaaaagtta
     3541 ccgcgatctt cctttgcgta tggcagaatt cggcagttgt catcgtaatg aaccgtcggg
     3601 cgctatgcac ggtattatgc gcgtgcgggc ctttacccaa gatgatgcgc atattttttg
     3661 tacaccggaa caaattgtat cggaaacaaa agctttttgt gatttgctca aagaaattta
     3721 taaggcgttc gattttagtg atgtgcgggt gaaattttca gacagacccg aaacacgtgc
     3781 aggatcagac gctatttggg atttagcaga aaaatcattg atggaggctt caaaagcagc
     3841 ggccctcgat tttacgctga accccggtga gggcgcattt tat
//
LOCUS       DAIAHG010000106         3881 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_106_length_3881_cov_3.221609, whole genome shotgun sequence.
ACCESSION   DAIAHG010000106 DAIAHG010000000
VERSION     DAIAHG010000106.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106_length_3881_cov_3.221609"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1105 a          792 c          777 g         1207 t
ORIGIN      
        1 ctactttaga aaaaatgaga aacttttgca attttttcga tgacacaacg ccttattatt
       61 gcgaattgga aaatgtttgg tgacccccaa gcgatcaatt attggtcaga caatcttgct
      121 gggacaaacc accatgttgt tttatgtgtc cagccgctgc ttatccaaag tgcagtcaag
      181 tgcgctgtga ttaaaaataa ttacagcgtt ggaggccaag actgtcattt taaagatgag
      241 ggcccgtata cgggctttac aagtcccaag cttttagccg atgtgggggc gagttatgtt
      301 ataatagggc atagtgagcg ccgtcatttc gaagatgatg cgctcattca aagcaaagct
      361 atcgctgcta tacgcaacca tatggtgccg atcatttgtg tgggggaatc cctggaaacc
      421 cggcaaaaag gccagcatga gcattttact ctcaagcaat tatctattat tttagagaat
      481 cttagcggtc cctattgcat tgcctatgaa cctttgtggg ctattgggac gggtgtcgtt
      541 ccaacgaccc atgatattga atctatgcat gcatccatac gacgctttat aaaaaacgat
      601 tccgtgaaaa ttctctatgg cggttccgtc tctgcagata acgcgaaagc aattttagcc
      661 cttaaaaatg ttgatggtct gcttgtcggt ggcgcaagtc ttaaggttga gacctttaat
      721 cagatacttc ttgcgatgta aaatatattt ttttctacgt cttcacgatt tattaacttt
      781 ttccatgata tcctgatctc ggtaagattc accttctatg tggagggtgc acagacggag
      841 ccggtttcat cgtagcgcga acgtggtgct attatgaacg aatgctgtag gaagtacaaa
      901 cttctttgca tccttcgagg gaaacttctt cggagtggtt agtcttgtgt cgtggaggag
      961 cgaggggagt ggccacttac caaatggggg tcgaggaggg ccccaccagg gaagcctatg
     1021 tacaaaaaat gtaaaaagga tttacgctat ctgcagagat cactgcttag gaaaaaacag
     1081 caactcattt ttaataaaat atttgtcaat tacgagtaag ccatcccccg ccaaggactt
     1141 tatctccttc ataaaaaacg cacgcttggc cgtaggccac accaaactca ggtgtatcaa
     1201 gatgcacttc tgccgcgata tcatttttaa gaaagatcgt agctgctaga ggctcttgcg
     1261 aggaacgtat tttaatcgtg cactttttcc cctgctcaaa atgataggta tccaccaaaa
     1321 gattaacctc tttggcataa agaatttttt tcgaaagagc ttctttgggg cctacaagga
     1381 ctttttttaa aggcgcatca agggcgatca caaaaagggg gtcaggatga gcgattccaa
     1441 ggccgcgacg ttgaccgatg gtataattga taatgccctc gtgatggccg agcacttctc
     1501 ccgttgcata atgcacaatc tcgccgggat ctagggcacc ggggcgaagt ttttccacaa
     1561 cctgcgcata gttgccattg ggcacaaagc aaatatcctg actgtcgggt ttttccgcaa
     1621 cttgaagatt aaagcgtttg gcgtgactgc gcgtttctgc ttttgtcata cttcctaagg
     1681 ggaagcgtaa aaaattaagt tgctcgcggg ttgttgtaaa aagaaaatag ctttgatctt
     1741 ttgatgcatc cacggctctg tgcatttctg cgcctctatt tgtttgaagg cgacgcacat
     1801 aatgccctgt tacaaggcat tctgcgccaa gatcttttgc tgtggataaa aggtctctaa
     1861 attttacttt ttgattgcag cgcacacagg gaataggcgt ttcgccacgc atataactat
     1921 ccgcaaaatc ttccatcacg ctttgtttga aaacactttc gtaatccagc acataatggg
     1981 gaaaccctag tcgatctgca acattgcggg cgtcatagat atcttgacca gcacagcatg
     2041 ctcctttttt cgccatgatc tcgccgtaat catagagctg gagcgtgatg ccaacaacgt
     2101 cgtatcctgc ctccacaaga agggctgcgg atacagagga gtcaacgccc cccgacattg
     2161 ccacagccac gcgccctttt tttgagatat catctaacgt aatcattttt tgataatttt
     2221 aaattatgaa tgctttttct ttatataaga agatttacaa aagatgcaaa catgtttaat
     2281 tttttttatt ttttcccgcc ttctctttat acttcttaat tttttcatca ttgatataaa
     2341 tatacttaaa aggagcgttt tctctttctt tttcaagggc ctctacaaat gcctctttgt
     2401 ttttatagtc ttcacgaaat ttttcactct gtttgaggtc attgtaaaaa agagtataaa
     2461 tttgagaaag ggtgataggc ccattttgac attcttctac aaaacctatg taaaggtctt
     2521 catccaattt attttttcca acataaaata cgttaaatcc ctgccaagaa ccaccatctc
     2581 tataagtaaa ataatccttg atatttttga tactacatga agcacctact gttggcgttt
     2641 ctgttatttc ataaaaggcg gtggccttac ccttatgttc cttttcccgg ataaccttaa
     2701 gaaaagcatt agagaaagaa atataagaaa gcccaatcgt gttaaaactg tttttttgag
     2761 aaaatagtgt tttgatatcc aaaatatgtt ttttgaatgc tgcgtcacct aatttttcaa
     2821 aaagaagaag tttttgtaca atgtctgctg cgactttaca ctcaatagtt cctgtgattt
     2881 ctggcaattt cttaaacgct ccgagaaaag tatctatggc gctatcccct gtatatttaa
     2941 ggaaatagcc gtttatttta tcatggaata gcgtaaaatt attggtgttt atttttgcat
     3001 cttcgttatt tttaggataa tcacaatttt tacagagatc gatcattgtc tttgtgacaa
     3061 attttatctc tttcttgttc agggtgtttt gagtatttat cttggttttc ttttccttgg
     3121 atatttcaag gtttttctct tcagacgcca aaagaggtgc tgtccctaaa agaaataata
     3181 aaaatagttt tttcataaaa ataaatcatc gttaaattta aatagtgaat acttaaacga
     3241 aatttattta ttgtcaatat tttttattta aatatattat gctgtgtgcc tttcaaggac
     3301 gtaacgtcct ggcgcacttt caatcacggg ttccttttga ggattcggta ctttaagctc
     3361 acccaaaaaa ggttgcatcc aattaagcca ataatgccac cacgatccct gatattccac
     3421 agcttcgtca aaccattcct ctgaggatgt gggaacagcc ttattcaccc agtattgata
     3481 tttgtccttc cacggcggat taataatgcc agaaacatga ccgctgccca caagaagata
     3541 ctctttaatc tcagacgaaa tcaattggga caggggataa acggattgcc acggcacaat
     3601 atgatcttta atagccccaa ggataaaggc gggggttttt atttttctaa aatcaatcgg
     3661 tgtgccggca atgcgcatgg ccccttcttt gacaagttta ttttcaagaa aaatatggcg
     3721 caagtattga ctatgcatac ggcctggaag attcacacta tcgcagttcc aaaaaagaat
     3781 atcaaaggcc tcaggcttgt gccccataag ataatgctta acataaaaag accacaaaag
     3841 gtcatgggga cgcagtaaat taaatgactg aacaagaatt t
//
LOCUS       DAIAHG010000107         3862 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_107_length_3862_cov_3.709643, whole genome shotgun sequence.
ACCESSION   DAIAHG010000107 DAIAHG010000000
VERSION     DAIAHG010000107.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107_length_3862_cov_3.709643"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1099 a          785 c          844 g         1134 t
ORIGIN      
        1 gaattagcac aatttcgatg aaaaatacag aaaaatgggc gaaaaaagct tcaaaatagt
       61 gaaattgttg atcaaaacag ggaaattatt ccgaaaatcg ccaaaattct gcaaaaatgg
      121 gcgaaaaatc acttttttcg tgaaaaatcg cttaaataat gtgcaaaagg gcataaaaat
      181 ggaaaattgc aggccttaat aactacctaa aaaatctatt tgatcaaaag ctctagaatt
      241 tgtaccacat tgagagccgc ccccttgcgc aaattatcaa aagcaatcca cagattaagt
      301 ccgtgacgtg tgcttgaatc atggcgaaga cggctaatga aaacagcatc ctcgcctgtg
      361 acttctgtgg gcgttgagta ggtctcaaaa ttgctcagca agcgcacacc cggagcattt
      421 ttaaggcatg attgcgcctg agcaacggtg atctcgctat caaacgtaat attcacactg
      481 gcacaatgcc ctataaaaac gggaacacgc acggctgtcg ctgtagttgg aatggcacgg
      541 tgcatgactt tttgaaattc actgataatt ttgatttcct cgcccgtatc ccccgaatcc
      601 tctatatcat caatatgggg aataatatta aacgcaatgg gcttgggaag atattcagcc
      661 tttacagatg tattcatata aatggcccgc gtttggttat aaagttcgtc catcgccccc
      721 ttacccgcac ctgaaacaga ttgatacgta ctcacgacaa cacgcgaaat tttcacaaga
      781 ttatctagcg gctttagcgc cacagcaaga gcaatcatcg agcaatttgg gcttgcaata
      841 atctttttat ccgcaagttt tatatcttct ggattcactt ctggaatcac cagaggaata
      901 tttttctcca tccgaaaggc actactatta tcaatcaata tagcgccagc acttgtaata
      961 cggggggcaa ctgtttgagt cagcgcaccg tcaagggagc ttaaaacaac atccatccca
     1021 tgaaaagcaa aagactcaag ggcatgaacg cgcagtgtat gctcatcccc ataagatatc
     1081 tcaccgcccg ctgaatgctg tgacgcaatc gctgtaattt gtgaggcgtg aaacccacgc
     1141 tcgtttaaaa tttggagaag tgtgcgcccc acattaccag tggcgccaag aacagcaatt
     1201 ttcatttttt tcttttgcat ttctgcctcc ccctagacat agaaagacgt tgctaagatc
     1261 tatgtctagc acaagtagcg atgaaattgt ggtgattttg cgggagaaca ataaaaaatg
     1321 tggctggggt actaggattc gaacctagga atgtcggtac caaaaaccga tgccttaccg
     1381 cttggctata ccccaaagat agagaattta taaacgaacg aggtcttttt tgcaagggct
     1441 tttccccttt ttggaaattt atttgcacct tttttgtgga atcttgttaa atttttttcg
     1501 agaaaattta aagtcagaag agagcccctt gcaatattag attttttagt ctatatttag
     1561 actaatcagt ctaaatattt ttggcccctg cacatgtcga tctatgcacc tcttgcgaaa
     1621 agtatcgcgc aacttttttc cccttttgtg gaaattgtga ttcacgatct tgagagcggt
     1681 aagctttctt ttattcataa tgctttttcg aaacgccagg tgggagatcc ctcgtattta
     1741 gacaaaaata ttgattggga tggtttgagt ctgagtgaga ttatttatga taaaataaac
     1801 tttgacgggc gcatgattaa atccgtttca attcccttgc cgctccataa tttcacacgc
     1861 cgggagcttc tttgtattaa ttttgacaca agcttttttc acacacttaa gccctttatt
     1921 gaccaagttt tagcgtcacc tcttttccaa gaaaaacccg aagcgttgtt taaggatgat
     1981 ctcgacagtc gtcttcatgc gttcattcat gaatatgtgc aaaaacatcg tcttgtttta
     2041 agcgctctta caaaggagca aaagcgcgcc cttctctatg atctttatga taaaggggct
     2101 tttgagcata aaaatgcagc aaccgttatt ggacgtattt tagacctgag ccgtgccacc
     2161 atttataact atttaaaaga catcaaagaa ttataaagga taaaaaaaaa tgacatttcc
     2221 agtttttaaa ctcgaagaat attttacgca ctatgaattt tcagcccctt atttgttgtg
     2281 ttgtagtgat gcgcaaactc tttctgtgag tgaattagtg gcccttgccg atgacgaatg
     2341 ccgtgacaag tgggagcgtc tttcccttgg ttacacagaa gtcagtggaa gtccgagcct
     2401 gcgccaggat attgcagcgc gctatcacgg gctgagggct gagaatattg tgtgttttgc
     2461 aggcgcagaa gagggtattt ttaccgtttt ctcaagtctt ttacgcaaag gggatcatgt
     2521 gattgtgatc acaccgtgtt atcaatcttt gcgtgatatt ccgcgtaccc ttgggtgtga
     2581 tgtgacgagc attgaccttt tggaagaaga aaaatggcag gtggatgtgg tgaaaataaa
     2641 gcatcactta cgcgataata cgcgccttgt gattattaat acaccgcata atccaacagg
     2701 acaaatgatg gatcaggaat cgttaaagac ccttatcaat atgctgcgca ctcatggtac
     2761 gtatcttttt tccgatgaag tttattttca tctgggcccc agtgacacac aatggtcgtc
     2821 gcaagttgca gaaatgtatg agcgcgggat aagccttaat gtgatgagca aagcttatgg
     2881 tttggcgggc ttgcgtattg gttggattgc aacgcaagac aaagagatcc ttcaaaaatg
     2941 tgtgtatttt aaacactata caactatttg caatgcagca ccttcagaga ttttggcgca
     3001 gatagccttg cgcgcacata aaacgattct tcagcgcaac aatcatatca ttgataaaaa
     3061 tttagagctt tttgaggcgt ttttagaaaa atataaaaat cttttttcct ggatcaaacc
     3121 caagggaagt tgtacaggct ttgtgcatta ttatgggcat gagtcggtgg atgattttgc
     3181 taaaagactt gtgcaaaaga atggcgttct cttgctgcct ggatctattt atgacgcagg
     3241 agatcgttat tttcgcgtag ggtttgggcg caagaatttt agtgatggac ttagtcggtt
     3301 ggaaaatttt ttagcagagg gcggacgatg attcttttca cttacgacga tattcaaaag
     3361 actttaaaga ttgagcgtga tttgggcgtt cttttagagg cgcaaaaaca agcttttatt
     3421 gatttttccg aaaaacgcat taccgttcca ttgccgatgc agctccattt tcacaatcat
     3481 ttgggcgaac ctgtgggcga ttgtcacgtt aaggcgggct atggcaatag tgaccatttt
     3541 attgtgaaag ttgccacagg atttcccctg aatgctgcgc atcacctccc ttctggtgat
     3601 gggataatga ttctttttga tgcgaaaaca gggttgatca aagcgctttt tcaagacaaa
     3661 gcttatttaa cgcatcttcg gacagccctg gctgtgtgtg ctgtgacaac gctaacacct
     3721 tttccagtgg aggcctttgg gattgttgga acagggactc aggctaaact ctgtgctcag
     3781 ctacttcggc acctttatcc cgaggccccc gtttatctgt ggggaaggtc tgaggataaa
     3841 gcccgggcga tggctttgga ac
//
LOCUS       DAIAHG010000108         3854 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_108_length_3854_cov_3.027800, whole genome shotgun sequence.
ACCESSION   DAIAHG010000108 DAIAHG010000000
VERSION     DAIAHG010000108.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108_length_3854_cov_3.027800"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1103 a          690 c          828 g         1233 t
ORIGIN      
        1 aaagtgaaaa ggttgcagtg gttgcaaaaa aactcgctga acatgtgatg gagaaagcac
       61 gcgctgagcg tattaaactg cccaatcgtc gcagtggtta tacacaaaaa gcctcccttg
      121 cagggcacaa aatttatatt cgcaccggtg agtacgatga tggccgcatt ggcgaaattt
      181 ttatcgatat gcacaaagaa ggtgccgcat tcagatcact catgcataat tttgccatgg
      241 cgatttccat tggccttcaa tatggtgtgc ctttggagga atttgtcgag gcctttacct
      301 ttacacgctt tgaacccgca ggatctgtgg atggccatga ccgtattaaa atggcgacct
      361 ccatccttga ttatattttc cgcgagcttg cgattagcta tcttggccgc aatgatcttg
      421 cccatgtgca aatcgcggaa agtgatcttc gtcccgatac tattggcgag cactctgatt
      481 acgatccgat ggtggaaagt gaagaggctg agggtattga ggctgtaaat gacaatggtc
      541 ttgtcatggc ggctgcaacg ggcacccatg gtgcggcgat cggtgtggcg gttgtctcct
      601 ctggcgatag acgggctgag gcaaaactca aaggctatca gggagatact tgtggcgatt
      661 gcggtaattt taccatggtg cgcaacggta cgtgcttgaa atgcgtaacg tgtggtgcga
      721 caagcggttg ttcttaacag aacagttttt aacaacaaac aacaaaagga ttttatttta
      781 tgaaaaaatt aacgctctcc ttattactcg cagctttctt tggaggcgct gcagcatctt
      841 caagtgtttt tgcctctgag aatatatatt ttcaaaggat caatgaggag gggaatgatg
      901 atgaattgat ggccggtgat acctatcttg aaaagatgaa aaaacttcat caagaaataa
      961 aaaatcagga gaccattaat gataaagagg aaaaagaaaa aacgcgaaaa aagctttggc
     1021 gtgagtgtgg tgagttgctt gaaaaagaac ctaaaacaaa agggtttagt gtgttgaatg
     1081 cacctatttc aatagataca gtcctttctg gaggaatgac ccgcgatttg gattatttgc
     1141 ttgaatttaa taggcaagaa aagattttta acaaatacac cacagtcctc caaaattggt
     1201 acaataaagt ccaagaaaaa tacaagtaca cagtggaata accttcgatg tgaggggggg
     1261 atttccccct caaaaaaatc tttgccttgc gcttcttttt cttttgattt agactatgga
     1321 tagtctttga aaggagtttc atcacatgga cgacaagaaa atttttggac aaacacgtac
     1381 aattgatatg tctcacggca ttgaccaagg gctcaggtcc tatatgctca atgtgtttaa
     1441 ttatatggcg attggtcttg ggatgacggg cttaacggct tttgcgcttt ccaattctcc
     1501 tgaaatgatg gccgctattt ggggcacagg ccttaagtgg gtcgtcatgc ttgcaccgct
     1561 tgcaatggtg tttttcttga gctttaagat tacaagcttg agtttttcat cggcacagct
     1621 tttattcttt atttatgcgg ggcttatggg gctctctctc tcctcgattt ttatggtctt
     1681 tacgggccaa agcatcacgc gcgtcttttt cattacagca tcactttttg gtgcgatgag
     1741 tctttatggc tatacaacaa agcgtgatct cacgagtatg ggctctttcc tctttatggg
     1801 gcttattggc attattatcg ccagtcttgt taatctcttt ttacacagct cggccatgca
     1861 atttgtgatt tctgtgcttg gtgtcattat cttcacaggc ctcactgcct atgatgtaca
     1921 ggatatcaag gctctttatc acgatatgga tgccagtgat acgatgggga aaaaggcgat
     1981 tattggcgcg ctgcgtctgt atcttgactt tattaaccta tttatctcgt tgcttcagct
     2041 tcttggcgat cgtcgctaga agtttctata aagtaacttc agaaaagaga gagccccttc
     2101 aattgagggg cttttttaat tcatcattaa ctttcgcgct gcaggatgta gtatgtaaat
     2161 tataaaaatg agataggttc atgattttac gtattttttt attaacgctc attctcatat
     2221 tgtcccctaa gctttgtgaa ctttgtgctg tggacgttat tatgaaaaac acgagcactg
     2281 aagatatcga tttttcaaac tgtaagcttg agtggtacct tgattgccag gataaagaga
     2341 aaaaaatcat gcctatttat ggagagccca tcgatttatc taggattaag gccttagaaa
     2401 caattgaact aaagggtgag tacttccaaa caggttttga ggatagcatc ttcgatcgtt
     2461 taacggttta tatttctaaa aataataaaa tccttgcaga gaccacgaaa aaactgatta
     2521 acacgaataa aaaaggccct attaaactgt tagcattaga ggttgctttg tatgagcaca
     2581 acgataaacc agaacttgac atctatatta ccaacgcttt atttgagagt gaactttttg
     2641 agccagagct taattctgat tcttatagag gcaacgaata tgaccgtttt ttctatttca
     2701 gagatccaga tgataagagg ctttactttt tgatgagtca tttagacgca acttatcgtg
     2761 aagctggtgg ctcagaaata gagagcgaaa gaaaattttt agaagaaaat acaaaaataa
     2821 gagacctatc taaagtaaat tttaagaata aaaaaatcta cgattataaa aatgagcctt
     2881 taaacgtaga gggagccctc tatgttcttg atggttttgg tattttacgc attatgcgtg
     2941 aggattatat ccctgctgag tttttaacaa aaaaaggtaa ggaatctgag tatttcaaaa
     3001 aaataacccc agaggatggg ccagattatt atacttttac aggtggaaaa cgtctttttc
     3061 atagtatgct tgcagcctct aaagccattg catgcggtgg ggaaatatgg gtgaaggatg
     3121 gaattgtcac aaaaattgat aatagtagtg gtcactatgc cccctcctat tggcaacttt
     3181 accttgccct tgattatctt gggttaatgg gggtgctcag taaaaagacg gaagtgggtt
     3241 tttataatga aaagggaaaa atggagtatc ttccctattt tgaatacaca gcacgtgggt
     3301 ctgatatgtc gcccatgccc aattatccta taaaaaatga atcgaaggct tggaaagaat
     3361 agggaacggg agccccccta atgtgggggg aatcattgtt agtttttctt attctgatta
     3421 tcgttgtact caatcatatt tttttcgagg gttaaaagat ctttttttac atcgaggagg
     3481 tctcttttga gttcaagctc ctctttattt ttatcaagct ttttttgaag atcaggaact
     3541 tttttcataa ttttattaat ctctggagta tttatgattt cagcaatttt cttaaattca
     3601 tttaatgtat taatatgtcc ttgagcatca tcgaggtttt ttaagtcttt attgaccttt
     3661 ttaagattct catttgtttc ctcaataact tcgcatttgc ccttgttttt attcatacgt
     3721 ttgagtcttg cttgtttctt tttttcctca aatgtatctt tttcaccctt acctgcaaag
     3781 ctgtcattga caaaaacact tgtgcaaaga acaagggacg ttaataaaat ttttttcatc
     3841 gtgtaaacct tttt
//
LOCUS       DAIAHG010000109         3831 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_109_length_3831_cov_3.425679, whole genome shotgun sequence.
ACCESSION   DAIAHG010000109 DAIAHG010000000
VERSION     DAIAHG010000109.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109_length_3831_cov_3.425679"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1156 a          745 c          739 g         1191 t
ORIGIN      
        1 acaggtgaaa aacagcccga tcccacagtc tatgagcaat atcagctggt caaaggccat
       61 gtcagagata tgattgacca ctatggcgaa gatgcaggcg tgcgtattgc gcgcaaacat
      121 gtggggtggt acagcaaagg attctatgat tccggtgatt ttcgcgttag aattaatcag
      181 gccacaaaat cctgcgttat gcttgattta attgatcaat tttatgaacc tcttgcatcc
      241 taacaaaccc caagaatttt ttaaaaatca aggattatcc gatcttgtga aagcattttt
      301 ggatcactat gttgaaaccc ttgaaggaga aaatcccccc ttcggtcttt atgatattat
      361 tatcagcgaa gtcgaaaagc ctcttttctt aaaaatttta gaactttgca aagggaatca
      421 aaaacaggct gctgcgattt taggcattca tcgcaatacg ttgcggcgca aaatggtcga
      481 ttacggaatg actgcataaa aaaaggcacc ctaagatgcc ttttcattgg tggaacctta
      541 tactaaagtt ttcctaagtc attgaaactc atgttgagga ttttcttaga aaggaaaagc
      601 tctttccctt ccttataata atcgaacgag cccttagtgc tgttgcgggc atattcaaaa
      661 ttttcttttg tgggtgaaaa ttgcaaaccc attttgagca tgacaagcgc gtaatgctta
      721 ggatccttaa taagttcgtt ccctaaaaac tttttacatt tctcattcac catatatcga
      781 aacatgtgac aacctaaatt gaacgcagat tgatagtttc catgattgac aagttttttc
      841 agagaatagg cagcattcat aatatctttt tgatttaatt tgatcccatt gccttgttga
      901 accacaaaag aagaaaacaa aaagtgcgca tacaggttaa gatcctcgta tttttccgta
      961 tttttaataa agccatggag tgcctctaac atttttttag gatttggatc gattgcgtcg
     1021 aagtaatcga atatttttag gcgaaggcgc gctgcataga atttgagacg ctcttcttca
     1081 tcataaaact tagtatcctg agaaagcgta aattctaata actttcttag ctttgtatcc
     1141 aaatactttt ctccaaaaag tgcgatagtg gtcgcagcgc gaagatcttt tttgcccctg
     1201 tctgtgtaag attcaattag ataatccatg atctcttttg aaggagaaag ttgcccccaa
     1261 aggtcatctc ttacgaaaaa agcagcgttg atttcttcaa atccataaga ttttattttt
     1321 tcaagaaagc gattttcttc atcacgacta tccttaaatt tctctaataa atcgttataa
     1381 ggagtaaagt ttattttctt ctgtaatgtg ttttccttat tctcgttatc tataacctcc
     1441 gaggcataaa cagaggtgtt aagagagagc acactggtaa aaaggataac tgaaataatt
     1501 ttatatgatt ttaacatttt ttatatttta ttaatgatta atagttaaaa tttgtatgct
     1561 atttttcacg atagatctat acaaaaaata attatttaag atcaaactct gattttttca
     1621 tcggtgtgtt caactgaata tccattaaaa tcacatctgt gataaagcct gccgtgtcga
     1681 tcgttgtcca ctgtgccaga tgaaagggag tgaccttaaa aataagtgtc agcgttgccc
     1741 cctccggatc ctcttttgtt gcaagagtaa tgagagcgaa cccatctttt tgaaccacat
     1801 gtttaatcac tgcatcccgc ttaaaatcaa ctgtattttt taaaattaac cccgcaggtg
     1861 tgctctcaat cgcaattggc agactcacac catcctcgtc cttttggcga aaggattccc
     1921 catctgaagc gacttgaagc agtggttggc cttcataatc aaagcgcacg taccctggtc
     1981 gcagccattt aagagagcca gtgcgcgatt ctgtcatgcc atttgggctt gttgtcactt
     2041 gaataaattt tcccttcagc gttttaatat tcgagagaaa attatgcgct ttggccaaaa
     2101 gatcctgctc gtttttgggc accatgggaa ttgcaaaagt caaatcatta acttctacag
     2161 gagcggtagg agcagaaggt gtcgctgcat atgcaaatga taacgagaat atagctgaaa
     2221 aaacagtgat aaggcgcatc ttaaacaata taaatgtgtg gaaatccttt gtattctacc
     2281 tttctttcaa aaaagagcaa tgtttttgat ttattcgaca tgattcatgc tttcttcatc
     2341 tttttttgct tctttttcca ttttttcaag cgctttgagg cgggtgttac tggggtgtat
     2401 attcgtctga tcagggaccg agctcaaagg aatataccct tccttttcat gatgccgtga
     2461 gcaagcggtg agagaaacga tagcgcacgt gaaaaaaaat atttttaaca taattttaac
     2521 gattttttag gaattaactg tcaccatcgt atcagaagac ccattgtaaa caacagatat
     2581 cccatgacag aaagaccgaa tgcaccccat gatttttatc aaatactggg ggatatgcaa
     2641 gatgtgcagt ggtctttttt taaagaaatg cttgcgattc atcaaaccat tcacacatca
     2701 ttgccgggat cactcaaaga aattatggat catggtcgca aaatagcatc gccacttaca
     2761 attttttcac cggatgttgt taacgataca ctggcaaaaa caaaagattt tttttacaaa
     2821 caacttgatc aaaaccccga caagctttgt gctattggtc ttaagcattt tgaaaaacaa
     2881 cagcgccttt atgtggaaac actctcaaaa gttatgggcg agcgctctga aattattatt
     2941 cactcccccc ttggggatcg gcgctttacg catccggctt gggacagcat cgcgtttttc
     3001 tcttttttaa agcagcacta tcttcttttt gtggagcttg tgcaagacat tatgagccaa
     3061 ttaaaagata tggatccacg aacaaaaagc aaaattgaat tctatacaaa acaacttgtg
     3121 catgcactgt caccgacaaa ttttcccgca actaatcctg atgtgctgtt agaaacgatt
     3181 cgcacaaagg gagaaaatat taaaaaaggt atggcgaatc ttttgaaaga tataaaaaac
     3241 ggtacccttc aaatgacaga catgaatgcc tttcaagtgg gcaaaaatat tgcggccacg
     3301 aagggttctg tgatttatga aaacgagctt ttgcaagtca ttcactatac gcccacgcaa
     3361 gaaaaagtcc atagcacacc tgtgctcatc attccggcgt ggatcaataa atactatatg
     3421 tttgatttaa gtggcgaaaa ttcttttgtg aaatggtgcg tcgatcatgg tgttgatgtg
     3481 tatatgatct cgtggattaa tcccaacaaa caccatcgcg ataaaacatt ttgtgattat
     3541 gctttagagg ggctgtataa ggctgtgcat gaggtgcagc atcactcaag cacaggaaaa
     3601 ataaacgcgt ttgcaaactg tgcggggggt attcttctca attgtctcat ggcgtatctt
     3661 gaggcacatc acattgaatc accttttgcc agttcatcca cgcttgcatc gcccattgat
     3721 gtggattatt taggcgatct caaagcgttt atttgtgatg cgcagcttaa aatgcttgac
     3781 gagagcttgg atgactttgg tattgttcct gggcaaattc ttgttcagtc a
//
LOCUS       DAIAHG010000110         3781 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_110_length_3781_cov_3.110421, whole genome shotgun sequence.
ACCESSION   DAIAHG010000110 DAIAHG010000000
VERSION     DAIAHG010000110.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110_length_3781_cov_3.110421"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1162 a          754 c          777 g         1088 t
ORIGIN      
        1 ataccacccg cgaacgcatt cagtcgcctg aacaacgtgc aaaattggat ggtctttggg
       61 agtgtgtgtt atgtttttct tgctcaacag cctgtcccag ttattggtgg aacggcgata
      121 aatatcttgg tcctgcggtt cttttacaag cttatcgctg gattgcggat tctagagata
      181 caaaaaccca agaacgcctt gatgcactta atgacagctt caaactttat cgctgtcaca
      241 caattatgaa ttgcacaaat acctgtccta agggcttaaa tcctgctaaa gcgattgggt
      301 ctattaagga aatgattgct gaaacaaatt aggttcaaaa gttcatgatt caaaacaaag
      361 atacgccagc tttttccgaa agtcacttaa cgtatgacga ttttctcaag gctcagcgtg
      421 atcttattgg gataacggaa gtcacgcctc ttgtcttatc accttcgctc tcttctctta
      481 tcgaaggaaa agtattttta aagtatgagc acacgcaaat cactcattct tttaaagcac
      541 gtggtgctta tatcaagctt atctctctca gtgagagtga acgcaaaaag ggcgttgttg
      601 cgatttctgc cggaaatcat gcaaaggctg tgtcttatca ttgcaaaaaa cttcatattc
      661 ccgtgactgt tgtgatgcct catgaaacgc ctgccaataa agtaaaggct tgtgaggatc
      721 ttggtgcaca gattattttt catggggcgc atcttgccga atcctttgag aaaggggaag
      781 aaattgctcg aaaaaaaggc atgaccatta tccaccctta tgatgatcct ttaattattg
      841 cagggcaagg caccgtaggc attgaactta tcgagcaaca tcgcacgcct ctcgatgccg
      901 ttgtgattcc cataggaggg ggaggacttg cggcagggat tggccgcgtt ctaagtgcac
      961 gctggcccct ttgtgatatt tacggtgtgc aaagcgagtt tgcaccagaa atggcagagc
     1021 ttctttttca ctatcgcaca acagcaccaa ggccttctaa aacaattgct gagggtatcg
     1081 ctgtcaaaaa gccaggggtg tatacacaag aaattttaaa agatgttctc tccgatattt
     1141 ttgtcgtcac agaagagtcg attaaaaaag ccatacacta tcttttgatc catgaaaaac
     1201 aggttactga aggtgcggga gcttcaggtg ttgcggcact gcttgctaat atccatgcgt
     1261 ttaaaggaaa aaatatcgca atcattcttt gtggcagcaa cattgatgcg gcttttataa
     1321 aggagtgttt ataaaagaaa tttttcattt attttcgata ccattctttt tttactacct
     1381 tttttcaaaa aaccttccta aaattaatct atcttttcaa agatacaacc catggagatt
     1441 ctcgcgtgat tcaaaaaatt ttatcaacag cccttctttt ctcagccgtt tctattttga
     1501 gttcctgtgc gcctaaagtc ggtggcacgg attattctgt gcgtggtatc ggtgaaatca
     1561 gccaaacatt tagtggtgtc atcgtttctg caaaacccat tacaattcaa gcaaaaccca
     1621 cagagcagca aggacagttg ggtacaggcg cactgattgg tgcgggcaca ggtgctgtgg
     1681 cgggatccct cattggtaaa ggcaaaacac cttggatcac aggggcgctt ggtgctcttg
     1741 caggcggtgc agcgggtcat cttatcgagc agaaagcaag tgagcaacag ggtatgctct
     1801 atgaggtcaa gcttgatcaa gggggctctg tatccatttc tcaaggttta gaacccatgc
     1861 tttctgtcgg ccaacgcgtc aaagttatcc acagtgccaa agaacgtagc cgcgtacttc
     1921 ctgaataatg ctgcatcttt aataagatta ttgtgaaaaa aattaagggg ggctttggcc
     1981 cctcttttta tgcaaaaaat gcttgtaaaa ttatttgaaa aaaggggtaa aatacattaa
     2041 tgataaaaaa atttaaataa tttaattgta tttatgggca aaaaatttcc tatgtctttt
     2101 attaaaaaat cacttttcat cttcttatct ctttccttgg aagcctctgc cgttcaatgg
     2161 catccgaatc aaacgtctct taaggattgg gtgcctgtta tacaggaaga ctttgccatg
     2221 acagttcttt ctaaggataa aaaatacaaa aattatgtaa aaaactttaa ggaaatgctt
     2281 aaagaaaaaa aagttagaaa gacagaaaaa tctttgaata aaaaagtcaa ctcgcagacc
     2341 cttcatctca aaatagctag cattatcaga aaagataaat tatttcattc gtccggttta
     2401 ggaggaaaaa cattttcaaa aatgtttcgc atctatcagg accttaaaca aaaatctttg
     2461 aggatctata gggaaatcct tgagcagcac ctgaaggctc cgattaaaaa caatgtttta
     2521 aaaacagaag atgaatccat taaaagctat tttaattttg tttttaaggc ggagcgaaaa
     2581 acagaatctg ccatacgtgg gaaaaaaatc ttcgtcaaaa cactgatcaa agaaaaactt
     2641 gcatcgatta gggggacggc tcattcctgg cgtcagtttt taagtactgt gccaaagctt
     2701 cgttcaaagg agcacgctat tttcctcaaa aagaaacgtg aaatacttca aaagcaaaat
     2761 cctaaaattc atttttcaaa aaaatttcgt agaaaaatgg gaaaactcgc tcgacatatt
     2821 tataaagagg agcgcttgaa acttgaaaat gatccgcaaa gatatagcga aattttgaac
     2881 actttggaaa aagttttaga gggcaacacc cctttttatt tcaattgtgt gtctaaaaat
     2941 taatttttta aaaaaccctg cttttagtcc ttgcaagttt tttggaattg agtatagtac
     3001 acacataaac gcgaaagcgt tgcgctaagc gggcgtagct caggggtaga gcataacctt
     3061 gccaaggtta gggtcgaggg ttcaaatccc ttcgcccgct ccattttttt ctccataaag
     3121 tgtatgacaa cgattttcaa aagctttgaa aaaagtctta catgcctttt cgaatatttt
     3181 ttccaacatc gcttgaaaca acaacgactt aagctcgaaa gcgatatgaa atgttacatg
     3241 cgtttctttt tcactgatag gcacaaagga ccagcgtgtc gtgagatgtt taaaggggcc
     3301 tttgacatgc gtggcgcaaa tattttcctt cccctcataa gtgactttgg aggtatacgt
     3361 ttccgtaaaa ggaccataac caatcgtcaa atcggcaaga aaatgatcac cttgatgatg
     3421 ataggtgcgt gctttaactg cccacggcaa aaaatgtggg tagtgatcga tcgctatcac
     3481 aacattaaaa agatcttcag ctgcataagg aagcgtgtga ctttcttgat ggcaaaaccc
     3541 tttagctttc atgttagttt tcatgacggc gcgcctgttg aagcttcaca aaatcctcat
     3601 ccgcgtgata agatgatcgt gtcaaaggcg atgatgccac gcataaaaag cccttcccac
     3661 gggccatttt tgcataatca tcaaactcat caggctcaac aaaacgatga acgggatgat
     3721 gctttggcgt tggctgaagg tattggccaa tcgttataaa atccacattt gctgcgcgca
     3781 a
//
LOCUS       DAIAHG010000111         3777 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_111_length_3777_cov_3.291081, whole genome shotgun sequence.
ACCESSION   DAIAHG010000111 DAIAHG010000000
VERSION     DAIAHG010000111.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111_length_3777_cov_3.291081"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1073 a          779 c          824 g         1101 t
ORIGIN      
        1 gtatttccag cacccctcat aagggcagga ctgttgccat cactcaaata aacctctgtc
       61 tcttcctcat caatatgttg tgtaatatcg agaagatatt tcgcattaaa gccaatttct
      121 acattttcct ggctatcata agcgacagaa agttcctcaa cagcacttcc aagctcggag
      181 ctcagtgcgg aaacagtcac aagattttct ttgagctcca gttttatcac acgaatttta
      241 tcactcacaa ctgtcgcaac gcggtccaca gcgctcatat agtcctttgt tggaacgtaa
      301 aaaatacgat tgttattcga aggaatagct tgaagataat cagggaaatt cccatcgata
      361 aggcgtgagc ttaaggagga tgtagcaaaa tggaattcga ttcgctgtgg cgagagtttc
      421 acaataacat cgccaccctc ttcatcaaga agtttgcgca cttcattcac ggttttgcga
      481 ctaataatca cacctggcat atctatagaa ccttcagggg ctggaatttg tgtaaaagcc
      541 agacggtggg catctgttgc aacagcacgc aaaagatttt ctcccgggat cgtatgccaa
      601 taaataccat tcagataata ccgtgtttct tctgtcgcca tggcaaaacg cggtttatca
      661 aaaagttttt tgagcgtttc agtcttaagc gaaaaagtaa ttggcaaatc cccaaaagtg
      721 agttttggaa aatcatcagc gttgaggtag gaaattttaa acttagagcg ccctgcatga
      781 atatgcagtt gattatcttt aacaaaaagg cgaaccgtat catccccaag cttgcgcata
      841 atatcaaaga acatagccgc tgaaaccgtt aatagacctg gttcctcaac aacagcgtta
      901 actttttcca caaggtttaa ttccatatcg gtggagtgga gtgtaatgct atcgccctct
      961 gcatggagaa ggacatgccc taagataggg agtgtatgac gtttttccac aacactttgc
     1021 gcatgggaga gagctttgag aatattattt ttttcgacgg ttaagcgcat ggggtcaggc
     1081 attcctttcg tttttctaaa tgtatgctta gtacttttct accatgtttt aaagaaggtt
     1141 tttaggtttt tctttggata atttttgata cgtgcatcgg aatttctatt aattcaatgc
     1201 tgacattagc attcgaggct tttgcgcaga atcgatagat cttcggcaag ttcgtgatct
     1261 gagaccatga gctcctcaac ttttttaaca gcatgaatca ccgttgtgtg atcgcgtcct
     1321 ccaaagcgac gcccaatttc tggcagtgaa cgcgttgtaa gcattttcgc aaggtacatc
     1381 gcaatttggc gagggcgtgc aatattttgt gtgcgcttgg ctgactgcat ttcggtcaat
     1441 ttaatactgt agtattcagc cacacgtttt tgaatatcat ccacacttac acgtctgtca
     1501 ttggacttta aaagatcacg caaaagttct tgggtgcttt cgatggtaat tttccgcccc
     1561 acaagtgttg catgggcggc aatacgatta agggctcctt caagctcgcg cacatttgat
     1621 gtaattttaa aagcaagaaa ctccatcact tctttaggaa tagggataga aagagtggct
     1681 gcttttgttt gcaaaatccc aaggcggagc tcataagttg tgggatgcaa gtccgccacc
     1741 agcccccacc caaggcggga tttgaggcgt tcttccatgc cttcaagatc cgacggtgac
     1801 ttatccgcag agacgataac ctggtgattt ttatctacaa gtgcattaaa ggtgtgaaaa
     1861 aattcctcct gtgtcgaatc cttaccactg ataaactgca catcatcaat catcagcaca
     1921 tcaacgttac gaaaaagttc tttaaacgtc atcgtgtctt tgtaacgaag agccctaata
     1981 aactgataca taaatttttc cgccgataaa taaaggacgc ggcgctctgg ctgatgcgtt
     2041 ttaatatacc acgcaatggc atgcataaga tgggttttcc cgaggccaac gcctccatag
     2101 agaaaaaggg gattaaactg tacctcacgg gattcggcga ctctaagtgc tgcagcatag
     2161 gcaagctcgt taggctttcc cacaacaaaa ttatcgaaat taaagcgttg atcgagctgg
     2221 gcttgaatgg tttctttcag tgctgcaaag tcatcatcat ttgctggtgt agctgttata
     2281 aagctagggg gttggggtaa gctaagtgtg gatacggggg attcttctgc tattggtgtt
     2341 ttgagtgatg ctgatgtatt cacgataatt tcaatgcgat gaacgttctc gtaaacactt
     2401 tgagccagtt ttaacaaggc gtgcaaataa ttggcatcga cccaatcctt tacaaaacgc
     2461 ccaggaacag aaagaataag cgttcgaatc tcaggatcaa aacgcactaa actgagctga
     2521 ctgagccaac ttttaaatgt ggcttgccca agttctcgtt ccatgagcgt acgcagtatc
     2581 tgccactttg atgtgtcata tttatccgca gagacaccat cgctctctag tgcctttgca
     2641 tcgttaagag gggatgagag agacaagtga cctggaagag gcaaaatgga gcacattttt
     2701 gaagagtgag tgacctagct ttatatttac cagacgtttg cctctaatag caagggggga
     2761 gagaaaaagt tattgtgatg gggggaaatg atctacttaa aaacgggaca agccttctct
     2821 atcaaagggc cccgtcctgg aagttcattg atcaaatgta tgataaaaaa actagtctcc
     2881 tgggttcttc agaggaatca cttggaaatc tttcacaccc atttgtaagg taacttcacg
     2941 atcagggcca atggcttgcc tgtgatcgcc aggggctgca tagcgtgcaa atacgagaat
     3001 gcctttgcct gtaagttggc taggtttaat attctggggt ccaagatttt gtccaggcac
     3061 gatttcatag gtataaatat ctatattatt tccagcgtcg cgtctaattt gctctttttt
     3121 ctgaaaatat tgggcagctg tgagtttggc gagctcttta agaagattat catcataaat
     3181 aatgaccaaa tcaaccgtta caggggcatc atcgttgagt ttttcatcga cctcaaaata
     3241 aatccgttgg agccaaacct ttgtaggttc catgttcaca gcagacttaa tggctgtcac
     3301 aatatcgccc ccacaaccac ttagaaaaag ggggatgcag gctaaaagaa tccatttttt
     3361 cataatctaa ttatcctttc agcatagcat taataaaatg agcgagcttg atatcttttt
     3421 cgctaatccc cttgcagtca tgggttgtga gggtaatgtc aagacgatta taaacattaa
     3481 accactcagg gtgatgattc attttttccg ccatcaaagc aacctgtgtc ataaaggaaa
     3541 aggcgctgat aaagtcagga aaaaggatgc ttttgataat tttagtgtta ttttctgaaa
     3601 gtttccagtc tgtgagatca tgcagtgcat tttctagggc atttttttcc aggggattaa
     3661 ccatttgttc atactttctt gataagctgt gtctataggt aggatgcctt aaagtgcttg
     3721 cattacgcaa gcgcatgcta tgtcaagaag acgagaaata tgggaaaatt tgactca
//
LOCUS       DAIAHG010000112         3745 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_112_length_3745_cov_2.868321, whole genome shotgun sequence.
ACCESSION   DAIAHG010000112 DAIAHG010000000
VERSION     DAIAHG010000112.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112_length_3745_cov_2.868321"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1164 a          645 c          791 g         1145 t
ORIGIN      
        1 caccgtcgat ctttatcaaa aatatattcc tgagatttcc tctaaaaaaa taaccaaaga
       61 aggccttgaa ttcatggatg attttatgga ggcatggaag cgaatttata gaattcatag
      121 taattttttt aatagtgaaa aaattgatga tccctactct aaaggtcctt ttaactattt
      181 ttcaacggct ctgtataatg cagaaaaatt ctattggcct cttgcagaag aaaaattgaa
      241 tgaaaaaaat ccgctcaaag agccgctacg tttccactct tttttaaaag aaaaaataga
      301 ttctgctgag gttgttgcaa atcttcagga ggaaaaaaag aaagatgttg attcttttgc
      361 aaacacagag acgaaggaaa aagtagataa tcctaagaag gaaaaagtag ataatcctaa
      421 gaaggacaag aaaaagcatc aaatcctata ctttttacgt ttttttcgat acaaataaag
      481 acacactttt ttatttttaa aatgctccaa tatgaaactt tctcaatttt tcttgcccac
      541 cctaaaggag gttcccgctg acgcacaagt gatttcccac cgccttatgt tgcgcgcggg
      601 gatgattcac caaacaagtg caggaattta tgcgtggctt cctttggggt atagagtttt
      661 aaaaaaaatt gaggatattg tttcccggga gcaagatatt atcgggtgtc aaaaagtaat
      721 tttgcctaca ttgcaatcag cggatctatg gcgaaaaagt ggtcgctatg atgggtatgg
      781 caaggaaatg ctgcgcatca aagatcgtca cgaacgcgat cttctctata gccccacagc
      841 ggaagaggtg attacggaca ttttccaaag ccatatcaaa agttataagc aacttcctca
      901 agtgctttat caaattcatt ggaagtttcg cgatgaaatt cgtcctcgtt ttggtgtgat
      961 gcgtgggcgc gagtttttaa tgaaagatgg gtacagcttt gatttggatt ataatgcggc
     1021 taagcaaacc tacgagcgca tttatgaatc ttatttgcgt acttttaaag agatggattt
     1081 gacagcgatt cctgtcaaag cagatacggg ccctatcggt ggagatctga gccatgagtt
     1141 tcatgtgatg gcacccacag gggaaagcac aatctattac gatgcggcct ttgagacttt
     1201 gcccgacgca gcgcgtactt ttgcgacgat gtctaatctc tatgcggcag cagaagaaat
     1261 gcatgatgtt gctcagtgcc caatcaaagg ggagcaactc aaatcctcta agggcatcga
     1321 gattgggcat attttctatt ttggcacaaa gtattcaaag gctcttgggg cagaagtcat
     1381 ggggcctgat ggtaaacaaa tgactgttga gatggggtct tatggaattg gtgttggccg
     1441 tcttgttgcc gcaattattg aggcgcatca tgatgatgcg gggattttat ggcccaaatc
     1501 tgtggcaccg tttgatatag ggttgctcaa tttaaaacaa ggcgatgcag cggttgatgc
     1561 gcttgcagaa gacctttata caacgcttcg atccatgaat gttgatgtgc tatacgatga
     1621 gcgtgatgag cgtgctggga caaaatttgc ggatatggac ctcattggcc ttcctcttca
     1681 ggtgattatc ggggctaaaa cgatcaaaga aggtttgttc gaggttaaaa tacgaaaatc
     1741 tggcgagcga aaaatgctct caaaagaaga actctttaac tttataaagg ctaattaaag
     1801 agagaaactt ataaacttag agaaattaaa aatttttcaa agcatttttc atctgttctc
     1861 caacagtaga aattgaatat ctatccctaa tatttgatgg acccttctta aggctactgt
     1921 tagaaaaatt atcaattatt tgcctaagtt tatttaaaat ctccgagata ttcagggggg
     1981 gcgctgtgat aaaacccatc tcttcaatga gtttttgaga tgccccagtg ggtgttatcc
     2041 caaaaagaat tttattggct ccaatcgcat caatgagctt gcttggcaag aaagggctga
     2101 tctttgctgg agcatcaatg atcaaaagaa tatccgcgga gtgcatgagt tcaaaagatt
     2161 tcttatatgt cacgggtggg tagataaaaa ctgtttcttc caagttaaaa gtaagcacta
     2221 aatcgtggag taaggttgtt gatgtgccaa taaattctac acgaatttgt gatcgatatt
     2281 caggggctaa ttgctgtagc gcttttaaaa aagtatcagg ctgtctcttc ccataaaaat
     2341 ttccaatgta gcgtaacgta atttttttat tagaagtact ctcttggcta atgtataaat
     2401 cgggattaaa cgtgtggggc aagcaaatag ttttttttct cacctctctt ggataagatg
     2461 cggtgacaag atcaattgtt tcggtcgatg taaaaagaag ctgatccgca ttcttaaaca
     2521 cactgtcttg atagtgttga ttcaggcctt ttgtccaaga attatagtca ttaaaactat
     2581 tatcaaccca cggatcacta aaatgggcta accatcgtat atggggatat tgttttttca
     2641 atttaagccc tgtgatgtgc gttgacatgg gctgcccaaa tgtcactata atatctggcg
     2701 atgaattttt aataatctcg cgagtttttt ttagaagatc aaaatgccaa agatattgaa
     2761 aatcgggcaa aattgctttt ttgaggcggt gacctttaga gcgctcaatc agctttgtaa
     2821 gaggtgattt ttttgcgtaa acaacgttat caagaggagt aaaacttaaa agagaggtgt
     2881 ctccttcacg tggaggctct gatgtaacaa ccgtaatctt aaaatctttg cgcagatatt
     2941 gcacaagatg gctgatttgc acagagcgag gaaatttctg tggaggatag caatacgtca
     3001 aaaataaaat atttaaacgt tccatcttct catccatcga ttcaaaataa taagactcca
     3061 tacttgatga gcggtgtgtt gatctccatt tttaagcttc tctagcaagg tatctaatat
     3121 atttttatga aaaaaatttg tgagatgttt agatgctaag ttctctttag caaaatcaaa
     3181 aagatctgac tgaatccaat gataggcagg gactggaaag ccttgctttg gacgtgtaat
     3241 gatctctcgt ggaattcttt tttcagcaaa agaccgtaga atatgctttg ttgtaaagcc
     3301 ttttgataat gatcccactt tccattcaag gggaagagtc gcacaccatt cgacaagttt
     3361 atgatcaagg aaaggcacac gtaattccaa agatgtcgcc atactcattt tatcagcctt
     3421 catcaaaaga tcctcaacaa gccagctttt acaatagact tcttgaagct ggtcaatagg
     3481 ctcaagtgat atgttttttt catagagttc ttggatgtat tttagagatg atttttgtgc
     3541 tgaaaaaaga gaaagttttt ttttctcatc ctcagaaaat acatttgtta tgtggattgg
     3601 attttctttc aaaaaatttg tgtaaccgac ttttgcaaga tttttaaggc ttgaaaacgg
     3661 aaaatgcttc aaaagagaag aaggtagaaa gttaaaaaac ttcaagcttg ttagtttttt
     3721 agctagagca ttaagattat accct
//
LOCUS       DAIAHG010000113         3737 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_113_length_3737_cov_3.217760, whole genome shotgun sequence.
ACCESSION   DAIAHG010000113 DAIAHG010000000
VERSION     DAIAHG010000113.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113_length_3737_cov_3.217760"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1147 a          945 c          645 g         1000 t
ORIGIN      
        1 tgctctcctt taaagttctt tgtcttcaaa tgttgcaaca atacaccgcc aatacacatc
       61 ctccttttga agcgcagaca cacgccgata gctttcacca caccatgtca cattatcaaa
      121 agatccagga tccaggcgca gataaagcat ataagctttg ctcaatttta tacgcccatc
      181 cacgacctcg cttgcagagg ctgcttgcac agcattttga agaggcaaaa ctttaaacga
      241 cgcccaaagt gttttagact tcttaaaact gcgtttcggt gtatcccctt catttttttg
      301 cacaaacgta tgaagtgtca caagctctct aaaatcattt aaacagaccc ccatctttta
      361 cccccatgcc attacacggt aaggttgtaa aagaaattgc accattccgc tcaaaccatc
      421 caccgcatcc acacgcttgt catacatttc tgcagcaacg agtgtgattg cctgacgaat
      481 cggcaaaggg atactcgacc ccgcttcgcc atagcctgct ttgtaaataa tttcaacgtt
      541 ctccgccaaa acacttaaaa aagggcggga cccgttaagc gcaacgctat agcgctttag
      601 agactttttc tcctcttttg ataaaatctc gctcacactg acaatatgca aaagaggcgg
      661 ataataaagc tcaacattat gcatagtctc tttttttcta aggtgatgtg acagtatgcg
      721 ccatgttttc accaaaagag tacgctctac atagttttca acagccgctg tcgccgtctt
      781 aattattgac tctaaataat tatcctccgc actgctctca aggcgaaggt gattttttaa
      841 atgctcgagg ggaataacat agccctcact ttcttcaata cagtcaatca taagttatat
      901 ctcctttaag agaatccccc tcattccttc gataaaaaga cagagagggg gtaaaaaact
      961 caaaacacaa aaatcctcgt gggaccttca ctaaaattga atgagtttaa tcgcatcaaa
     1021 attaatcaca tcgccgccga tacgttttgt cgcatagaac tcaacatatg gttttgcaga
     1081 gtatggatcg cgcagcaact gtatgtcctg acgttcgaca atttgatagg cctgtttaaa
     1141 attaccaaaa gcaatcgaca aactctcctt cgaaggatta ggcatcgcat cacaaataat
     1201 cacaggatac ccaagaagtg tgcttgagga ttcttgagac acatgcggaa gccatacata
     1261 atgaccatct ttatccttca ttttgcgtac cattgccagt gttgcccgtg acattatcca
     1321 atgagcaccc tctaaatact ctggcttcat gctatgaaaa acctcgacaa gaacatctgt
     1381 gtcttcaatt ttaccttctg aaaaagaaga cattttctca atctttcccc actctggcgt
     1441 cccaagatct gtggtgggat gcgtcaaaaa accacgcggt ttgttcttac catcaccatt
     1501 gacaaaagct tgtgtttcca tgatgcccat ctgcattgca attttttgaa taagccattg
     1561 ctcaatattt acacacgcat catcaagcag tttttgactg gcccttggac gtgcatacat
     1621 ctcaaaggta ggaatcgtaa ttttagcaag ctctggcgcc tctgtctcat cacgacggcc
     1681 tgtctctgca acccaaccca catctgcacc acccttatcc acaaggagct ccaagctctc
     1741 agttgcaatt tgcgatactt ttgcaatttt acgtaaagga gaataatcag ggataacacc
     1801 ctccacaaat tcctgtgtac tgttgggaat taaaatcgtt tggctacgac tatccgtgga
     1861 tgagagggat ttttgaccat aatcatctcc actgcgtaaa aaatgttgaa acatatattt
     1921 atctccttgc cgatgatgag aattagttga aaatccactt ttttgaaaaa gagtgtttgg
     1981 ctgtgaagta gtgtgcccct gcactgatgg ccttttatac gccgattcaa gggcactcag
     2041 ccgtgcttcc atttttgaac aactatgaat cgttgtattg aaatgggtat tattttttgg
     2101 ctcagcatga ttcatttgca tgacgatttc tccttatttt tgtgtgaaaa atataaaaaa
     2161 aattatttta aaagaataga aagtggattc gctggacctt gaacaatcga aatttcaata
     2221 agctcaagct cctgaagaat acgtatacca ccctccctat acgtacggaa gacatcaaaa
     2281 ccgatggata atccccgtgc ataacgaaaa gatttctgag gtgaaccata ccatcgctct
     2341 cgaaagatcc cctcaaccca aagtccatga gcgtcctcct cgatcaatga ccaaaatcca
     2401 atctcatccc cgggttcatg ctcataaaac atgagtggca tagtactttg tgatctatgg
     2461 cgcgcgagtg aacgtctaaa tgcacccgga gcgacaagat cattttgacc atcgaccacg
     2521 ccaaaaacac tggcataacc acaaagaatc tctcccataa aagtctcctt tcacgtcata
     2581 aaagtgttca tttgatgaaa aaataaaaaa tccccaacac tcaaaaatga aggggaaaac
     2641 gtaaaaaaag attattcgct aaaataacgc ataccaacgc atcaacttaa aatttctaaa
     2701 ttcgatcgcc attttcaatc ggaggatagc ccacctcctg acgcttttca ttgagggtta
     2761 aaaaatctgc acgcataatt ttatcccaat gctgttccct tttttccgcc agtgctggga
     2821 tttcatccgt attataacgc aagacaagat tcccctcaaa agactgtgca agccacgtac
     2881 tcagctccac tgtcataaac tcaagcaaag gcaaaatcgt atcctcccat aaatgaagac
     2941 gcgcttcttt ataattcgaa tacgtcgcat ctcccataat gcctacaaga atcgaaggca
     3001 caccaaaggc ctgagcaatc tcccgtgcag aaatattttt accctcgaca aaatccaaat
     3061 ccttagggga taaacccatt tccttccact caaaatcccc ctctaaaatc ataattttac
     3121 ctgcattttc accgccctga taaagccgcc ttaaatcttc acgcaaactc tcccgttgat
     3181 caggactaag attttgttcc ttaagcatca aagcacccgt cggccttccg ccattttgaa
     3241 gaagtgacaa attatgtgac ccgacctgat tgtgctgatc aatagaactt gccgctgcct
     3301 ctaatggact caaaccgtaa taatcgtgaa gaggatgaaa caatttgata tgcaaaatgg
     3361 gcgagggtcc tgaggaagga acaaccacac gctttacatt gccacccaca cggtattcat
     3421 acgccgcaat ctcaccacca tcacccggta caactgtcat acgatctgga cgaagcgcat
     3481 aaagttcaat aggcgcgcca agatcattat aaacagcctc aatataggta ttgccagaca
     3541 gcaacaaata actcaccaca tcctcaataa aggcagaccc tccttgcaac aaggaaggat
     3601 gatgcaatag atccaaaata ggatgctgat aaatttcccg ctcatttccc tccttatcgc
     3661 gctcatacaa aagccacggc acacttccca tcgcccgtgc aataaggttg acacagcggt
     3721 acacaataac atttttg
//
LOCUS       DAIAHG010000114         3735 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_114_length_3735_cov_3.601422, whole genome shotgun sequence.
ACCESSION   DAIAHG010000114 DAIAHG010000000
VERSION     DAIAHG010000114.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114_length_3735_cov_3.601422"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          956 a          742 c          912 g         1125 t
ORIGIN      
        1 gctgttggct gttggctgtt ggctgttggc tgtgcatggg gttttccttt tattttaaat
       61 aaattctact aacattttaa tttatatgat ttttcaaggc acagcgccat atgttttatt
      121 tttaatgcag ttgccccacg gagtgtttat aaagagagca tgaaatcttt taaagactaa
      181 ggtaaagggc atgctacagc ccccaagaga ttacattgtt tttgcccgta aatatcgtcc
      241 tcaaacgctg agagatcttg tgggccagga agttttggcg cgggccttga gtaaagcgat
      301 tgcagaaaat caactgcccc acgggattgt cctgagtggc atgcgtggga ttggtaagac
      361 aacaactgca cggattattg caaaaggatt gaactgtgat gtggggccaa cggtggatcc
      421 gtgtggcgtt tgttcttcgt gtattgcgat taccagtgat cgtcatttgg atgtgataga
      481 gatggatgcg gccagtcaca caggcgtgga tgatattcgt gaggtgattg agacatcgaa
      541 atacaaggcc gttcaagggc gctataaaat ttatattatc gatgaagtgc atatgctttc
      601 aaagagtgct tttaatgcgc ttttaaaaac ccttgaggag cccccgccct ttgtgaagtt
      661 tatttttgcg acaacagagc ttcaaaaaat acctgaaaca attctatcac ggtgtatgcg
      721 ctttgatttg cggcgtatgg atgtgaaaac gatttgcgcg cgcatggcgc atattacaaa
      781 tctggaaaaa atcactctcg agccggatgc ggctcttttg attgcccgtg ccgcagaagg
      841 ttccatgcgt gatgcactgt cgctgcttga tcaagccttt gttttatcca aagatcatca
      901 gattcaaacg gagcacgtgc gtgcgatgct gggcctttca agccgcgatc atctttatgc
      961 gcttcttggg gctgtgtttc aaggtcaggt gactaatatt ctcgagctga ctgaaaatct
     1021 ttatgagagt ggcggtgatc cattggtatg cctcaaagac cttctcgatg ctctttacgg
     1081 gatgatatgc gtcaaaacaa atcctgcgct tttagaaaaa aataccttta cggagtcaga
     1141 aaaaaccgcg attactcctc ttttgcaaaa tatgactctg ccctctttaa tgcaaatatg
     1201 gcaaattctt aacgaccgct ttgataaaat atacaaggcc ccctttccag cgcaagcttt
     1261 tcaagtcaca ttaatgcagg tgtgctatat agggcagctg ccgcccttgc gtgatattct
     1321 tatggaaaat gctaaacctg aggaaaaacc tgagggaaaa gtgattgaaa aacctctcat
     1381 gcgtgatgca tccgagggga tgttagattc ttcttgttca agtgctcaag ggctttcatc
     1441 tgttgaggca tttttggatg tgttgaggga taaacgtgag ccgattcttt acgcgcaggt
     1501 gtgccgtgac attgcttttc agtcttttaa tcatgggcat gtggcttttt atacaaagcc
     1561 aggagcacct gcgaacattc agcaacagat caaattattt ttagaaaaac agacggggca
     1621 gtcttggact gttgagaatc ttggcgtttc taaggatgcc ctttcaatcc aagaaaaaaa
     1681 tgatgaaaat aatcgtgcgc aacaagctca agcactaaag catccgcttg tgcaggatat
     1741 tctcgcatct tttccagggg ctacggcaaa agttgagggt gctttatagc aaatttattg
     1801 tgaaagtgcg gggcttagcg agttttggat gatgaaagtc ttttgatgga ccgggctgca
     1861 tgcccaattt ttccacacga agagatctta ggttttgagg gggagctgaa cgctataatt
     1921 tttttaaccc gcatcgtgta aaaccatctt gtagcacagc ttttgcgccc tctatggtat
     1981 aactttttcc ccagtattgc gcaccaaggc gccaagaaat ttcagtgtat gaggagaaag
     2041 gagcttctaa ctttaaagga tctagaccta taaatttata agctcttggc tttttttttc
     2101 tccgccgcat agagtgtgtg tccaatgtca taaaattggt gatccttcca ggtctgaaga
     2161 atgatgcgct ctgtcttgag gatggttgat attggattgc ccatgaaaga tataatgcgt
     2221 ttccccctct tgcccattat gatggatgta agaaggggaa aacagggctt tagaatccgt
     2281 ccctgtccat acgcttgcgc ataagtttac ggtgacgacg cacagactca gccttttcac
     2341 gttttttacg tgcagaaggg gtttcgtaac ttgtacgaag tttcatttcg cggtagatgc
     2401 cttcgcgttg catctttttt ttcaaagcac gcagcgcctg atctacatta ttatcacgca
     2461 cactcacttc cattaatatc aactcctttc ctggccaaag ggccatactt gaggggaatt
     2521 atagactaaa aagcatagcc ttttcaagaa ttcttatgat tattttctaa tgcacagaga
     2581 gtgtttgcac acttttttgc aaactttctg tgtgacgcaa gagtgtgtcg acgatttttg
     2641 tcatatccac agatgtctct gaggcttctt tagcagcggc atttacgctt gcaatgcgtt
     2701 ggctcacatg ttgtgtgccc ataagtgctt ggtgaatgtt ttgggaaata ttgtgcgcag
     2761 agcgtccttg ctgctcaatg gcaagggcaa tggcatcaga aacatcgttg acctcatgaa
     2821 taatcgtgct aatggagcga atggatgtga cggtatcccg tgtcgctgct tgaatgtgct
     2881 caatttgatt gctaatgtcg tcggttgctt tggcggtttg attcgcgagg tttttcactt
     2941 cggacgccac aacagcaaac ccttttccag cttcaccggc gcgcgcagct tcgatggttg
     3001 cattgagggc taagaggtgg gtttgattgg caatatcttg gatgagctgc acaacatcat
     3061 taattttgga tgctgcatcg gcaaggccat tgacgcgcgt atccgtctct tcagcggctt
     3121 tagcagcttg tttggcaact tgtgccgatt gactgacgtg tttgcgaata tcttcaatgg
     3181 agccactgag ctcattagct gcggagacaa ccgtttgcac actgctattg gattcttcgg
     3241 ctttttctgc agcagaggca acagtattgt aggcgttgtt tgtgtgatta ttaatttctg
     3301 tggttcggat tttaatgctg gaggcaagtt gatttaatgt atcgcacaca ttttttgttt
     3361 gatgcttaaa atgatcaatt ttttcaagag tatcgttaga ggtagagggc ggtgtataag
     3421 actcctttgg gggggcaatc tgaatttcag acacgtgggc ggattgagcc aagagcgctt
     3481 gttgccgttg gtattccaga atatgggaga ggtgccccac ttcatttttt tcctgctgaa
     3541 agggcagggg aatgtgaaga ttgttttgca aattttcaac agcattactc agttgtaata
     3601 tttttgagct tgtttgatag ccaaatccaa tgataaaagc gctcagcata accccaacaa
     3661 aggggaaaaa ataaaaagca acgacggggg ggagatagct gaaaaagaac gtatcacagg
     3721 taaagaggaa tccac
//
LOCUS       DAIAHG010000115         3725 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_115_length_3725_cov_3.077029, whole genome shotgun sequence.
ACCESSION   DAIAHG010000115 DAIAHG010000000
VERSION     DAIAHG010000115.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115_length_3725_cov_3.077029"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1159 a          857 c          700 g         1009 t
ORIGIN      
        1 aaaaacgcca aatttagtga aaaaatctct tttttcaagg aaaatccttc aaaaattacc
       61 tgaaaaacac aaaaaatagc gaaaaaaata ggattttcga aggaaaatct agtggcgcac
      121 tgataagaaa aatttttaaa caaaatctta ttaattgatt ggcctactcc cctcttttca
      181 agcagccgtc gcgagaccat cgaggaaatt tcgcgttaaa atcccacctt tatgctgttt
      241 aaaatagtct tgaagccttg aaatctcttg gtcttttaga tcctcgagaa tttgagactc
      301 aattttatag gaaccataaa ttctgctttc gatccgtaac aaaggatgca tataaacagc
      361 cgccccttga acacttgtcg gccactgcaa tgggcaaaat tcctcgagca tgactgtcac
      421 aaggtggggc atcacggaca ctattccctt ttgatcttta tgaacattca tcgagaaaat
      481 ctctgcctta tcatgaaaaa ttttaatagc ccccgagcgc aatgttgaga aaaaaggaag
      541 atgccccacc atcacaccta tatccccctc aacagcagga caaacgacca ttgtggcatt
      601 ctcaagccac agcagctctg tgtaaggtgt tagaattttc aaagaaagca tcgcaggatg
      661 ggtcataaaa agcatacttc ttagcagcat gaatcgcttt gtctatgtta gcggtcttgc
      721 caaaatgatt caaggccttg ccgagagcac tcaaacataa aataatgttg gaagatctgc
      781 acgtttgtcc cattgctcct atacgtaggc ttttatcttc ccattctcca aggccattgc
      841 ttatctctat atgatggtga tgggctataa attgacgcaa ttcagcccct gatagtccct
      901 ctggaatgtc aagtacgttc accaaaggca acctctccaa aggattttta acacggtgct
      961 tcatcccaag aatctcaagt ccctgtgaga gcgcttttgt attcattgaa tgccgtgtcc
     1021 acacagactc gataccctcc tcatagatca ttagcaatcc ttcatgcaga gcataaaaag
     1081 aatgcgcagg ggatgtgtga ggataaaaac gcttcacagg acatatatat cctaaatcat
     1141 agatagcact taggtttaaa caccaatttt gaacaggcgt ttttcgacat tttattttat
     1201 gaagagccct tggactcaaa ctcaaaggtg ctaagcctgg cggacaggac aaacactttt
     1261 gaggatttgc ataaacagca tcaagccccc actcttccac atataaggga atacctccca
     1321 gcgtggctgc actatcacac aacgttaagg ccccatattt ttcagctatg gtggcaatac
     1381 tcttgatatc ggacacaaca cctgttgagc tctcaccatg cacaaagcaa atgagtttta
     1441 tatctttttc atcttttaaa gcctcttcaa gacgctcagt tgataaagcc tccccaaggg
     1501 gatgagtgag cattgtaatg cgcgcaccat aaatagtcgc catttctttc atacgctgcc
     1561 cgtacaaacc attgctcaaa atgacaacat gatctccagg ctcaatgaga ttcgccaggc
     1621 acgcttccat ggccatcgag ccagagcctt gaatggggag tgtcatggga taggatgttc
     1681 gcatcacata ttgcaaaagc tcccgcgttt cgaaaaaaag tctttgaaat tgtttatcga
     1741 gatgagagat cagagggcgt tctaacgcct ttgatacacg gggatgaagt gtcactggac
     1801 ccgcacccat aaaaagctga gaagaaggga taaaggatgc gtaggtcata ttctaaaatc
     1861 caattgatgt gtatctaaga tcaagactat gatgcaaaaa ttaatgaaaa attaatagat
     1921 agctctgcct ttcttaacaa aagaaagaga tgtggataat aagttaacat caacaaaaaa
     1981 aggggaaaac cctatgattt ccttgttctg gccatagggt catgaatgac ttgattaatc
     2041 ttttcactga ttgcctcttt tctccctagg gtgaaaaaaa tagatgtttt ttcaaagaaa
     2101 gatagaaaag aatttaaaag atttaaaaca aaaagctcta aaaaaatact catcctcgta
     2161 taaaatcttt gtgcatcaaa taatcctcaa catattccat cacaacatgc cctgctgtga
     2221 tatgggattc ttggatgcgt gctgtgtttt ccgatggcac cacaaaggcg tgatcacaat
     2281 gagaaagcat ttgccccccg ctccccccta aaaagccgag agcatgaacc cccatatcct
     2341 ttgccttttg aagcgcttta atcacatttt ttgaatttcc tgacgttgag atccccaaaa
     2401 gacaatctcc ttttcgccca aggccctcaa ggcttctgga aaaaatctca tcaaaagaat
     2461 aatcatttcc acaagctgtc acaaaagaca tatctacagc taaacaaagg gctgggaggg
     2521 cagagcggtt cacatgagga tgaagacgca ctaaaagctc cgccgctaag tgctgtgcat
     2581 cggccaatga ccccccattt ccacacaaaa aaagcgtgcc tcccttttcg atagatgacg
     2641 caatggtaaa agcaatcgtc tcaagatttt ctaagtgatt ttctacaacc ttctgtttca
     2701 cggccaccga ttctaaaaat gcagcacgcg ctttctcaat aaatatcaat tgactcattt
     2761 cctttcattt tttatgcact tttttgtgct tcgaagtaga gtgaatagcc cacgcgatgc
     2821 ggtccttcaa taagcacatc cctatgacgt cttttcgttg tattaagatg caccaagata
     2881 atcatcattt tccacgctta tcccaaaaat atgagtagac ccgatcataa gcgtccgcac
     2941 acttggctgc attaaaaagc ttaaaagcac gctcttgacc taaacgccct ttttctttca
     3001 gagtattcct atctttcgag gcttgtacaa tcgttttacc aaggacttta ggatcttttg
     3061 caggaacaat ccaccctgta gtattatcta aaacagcctc gctattccct ccgacatccg
     3121 ttgcaatgac aggcaacgaa gcccccatac tttctaaaat agcatttgaa aatccttctt
     3181 catgggaggg caaaatactt acatcagcga gttttaaaat gtccggcaca tccgatcgtg
     3241 cgcctaaaaa atgaatatat ccgtcgattt caagcgaaga agcatgggct ttaagagcat
     3301 ccatgtgtcc atcatcacga ccgatacaca aaagtcgcca atcttttggc aaatcatttt
     3361 taacatgcgc aagggcatcg atcaagtcct tgtgcccttt ataagggata atgttcgcca
     3421 cgcacaccat cacaaagcaa tcttctgtga gattaaaatc tttgcgcgta tgagttgaag
     3481 ggttctcaaa gggcataaga tcgataccat tataaataag ctgcagttgg cttttgggaa
     3541 ccttttcctc aagatgaagt tgatccatca cagcctgact gttcgccaaa ataagattca
     3601 tacgcttgtg caaaaaacgc tcaagcaaaa gggttgtttc caagtcaaga tagttggttg
     3661 gctcatcaag aagcatcagg tttgggtcga tgccgagaag tgcaatcagc ttgatgcgca
     3721 tacga
//
LOCUS       DAIAHG010000116         3705 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_116_length_3705_cov_3.578831, whole genome shotgun sequence.
ACCESSION   DAIAHG010000116 DAIAHG010000000
VERSION     DAIAHG010000116.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116_length_3705_cov_3.578831"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          982 a          714 c          822 g         1187 t
ORIGIN      
        1 gagaagcgca ccattcggat ctctttttgc ggaaataata gggtgaaaaa gattatcaac
       61 ctcaaggtga tggtgactaa gcgcattttt aacagtctcg agtataaaag ggcgatcttt
      121 ttgtaaaaga ttgagggaaa gttttttgcg ctttttatcg tagtgataac ctagggctaa
      181 aggcgtttta aagggttttt gataaaattc ccaggcacgg ttcacttttt ctaaaagtgt
      241 atgatcctta gaattgtcaa ggagttggct cggtatacct tcaaaaaaac atgcaacaaa
      301 gtgaacccaa tggggatgag atccctggta ggataggctt tgaaattttg agacgaattc
      361 ttttttgcgc atgagtaatg ttttcatggg atcacgtctg gcacaacgcc tctctaccct
      421 aaatgctata gagaaaagat taaaaaaagg taaagcagac ttttttttaa gcagggtgtt
      481 tttaaaaaat gtggaaaaaa aataaaaagc agtgaagaat cagatgaatt attttttggt
      541 gaaattttag cgtatgcctt tttttccttt tatcaaagct gaagcgcttg gcaatgactt
      601 tgtgatgttg cacaaaaatc actttaagaa tgataaggtt ttcattcaaa agcttgctga
      661 tagacgtcgt ggcattggct ttgatcagtt gattgtctat accgacgatt gtgagattcg
      721 cttttttaat agagatggga gtgaggctga ggcttgtggt aatggtacgc gctgtgtggg
      781 attttttctc gcacatcaaa ggcaacaaaa gtcccttacc ctcaaaactt tagcgggacc
      841 tctctcgctt actgtgttaa aagatagcta tccctttggg caagtcaaag tcacgttgca
      901 gccgccaaaa cttatgctaa agacagagga gcttactcag gatattcact ggttttttga
      961 tcaagagaat tttataaata tctttgcggt ggatgttgga aatcctcact tgattctttt
     1021 tttacaggat atgcgtcatg tgaatattca agcgatcgcg ccacaacttg agcatcatcc
     1081 tttttttaaa cgtggagcta atattagttt tatctcaatg aaaaaggagg gagagctttg
     1141 ccttcgtgtg tgggagcgtg gtgtgggcgc tacgcctgcg tgtgggacgg ctgcctatgc
     1201 agcgtcgttt agctatcatc ttttgcaacc tcaaataaaa cacgtcaagg ttgtgcagga
     1261 ggggggagaa cttttttttg atcaaataga ggatcagtgg tttatgacag gagaagcgcg
     1321 tatcctttat gctggggaat tttataatta aaggaatttc cctaaaaaat agcattttaa
     1381 tgcgttatta ttacccttta taaaaaatta aaaacaatta acactaccat ttgtgcgtat
     1441 aaatccttat attagaatgg aaaattttca cacctcctta aaatagaaga atgacaagcg
     1501 ctttaaaaaa agatagtgat acgattaaaa ttgtaacttt tggatgccgc ctcaatacgt
     1561 atgagtcaga ggtcatgcgc aatcttgcca cagaggcggg gctgagcaat accattcttg
     1621 tgaatacgtg tgctgtgaca aaggaagcag agcgtcaggc gcgtcaatcc attcgcaagc
     1681 ttaaacgtga aaatcctgag gcgaacatta ttgttacagg gtgctctgcc caaattaatc
     1741 cgacgcagta tggagagatg gcggaggttg agcgcgttct tggcaatgat gaaaaaatga
     1801 agcttgagag ctatcaaaat aagacagagc gggttcttgt caatgatatt atgtcggtga
     1861 aggagacggc agcgcatctc attagtagtt ttgagggaaa ggtgcgcgct tttattcagg
     1921 tgcaaaatgg ctgtaatcac cgctgtacgt tttgcacgat tccctttgga cgcggaaatt
     1981 cgcgcagtgt gcctcttggt gaaatcgtgg cacaggtcaa gctgctcctc gcatctgggt
     2041 ataaggaaat tgtttttaca ggtgtggata ttacggacta tggcaaagat ttgcctgctg
     2101 aaccaacatt agggcagacg attaagcgtt tatttacctt agttcctgag cttaaacgtt
     2161 tgcgcctttc atccattgat cccgttgaag ttgacgctga tctttgggat gtgattgcgc
     2221 atgaccaacg tcttatgccc cacctccaca ttagccttca ggcgggggat gaccttgttt
     2281 taaagcgtat gaagcgtcgc catctgcgcc aagatattct tcatttttgt gaaaaagcac
     2341 gctctcttcg tcctgacgtt gttttcggcg cggatattat cgcaggcttt cccacagaaa
     2401 atgaggccat gtttaaaaat actttgaata ttattaaaga gtgtgatctg acatatttac
     2461 atatttttcc ttactcaaag cgccctggaa cgcccgcagc ccgaatgccg caagtaaggg
     2521 gggatgtgat caagcagcgt gcagcgctct tgcgtcaagc gggtgacaaa gctcttcagc
     2581 attattttca aaaacaagtg ggggcagtgc atactatgtt agttgaagaa gtcacggatc
     2641 aaggcgatca tcttctcatc aagggaaaaa cagatacgtt tgcgccaatt ggtgtgcctt
     2701 ttgataaaaa atcacaggtc ttgccagagc caggcgatct tctacctgtg cggatccaaa
     2761 acaccgatga gggacgtgca ctcatgggcg ttttggtgta gatcagttct tttatctttg
     2821 gttttggcaa tagaaaaggt cggctcctac tttgccttta cacttgtctc ctttattgtc
     2881 attgccgcca ctacggttat tattttctcg ctcgttgttt ccgctttctt gttcatcagc
     2941 gtgggtgtat ccaatggtaa cctttttttc atccttaagc gggtttatcc caatcatttc
     3001 accttttaaa aggtttaagc agtctgtcgt tgcaaggttt ttagggagaa tatcgatctt
     3061 tctgtcacga aaagctttgc atatcctatt ttcccatttt tcataaggtc caggctctcc
     3121 tgacttgggt tcaaaatctt cacacttctc aacaacttca tcattcataa atttattata
     3181 ttttaccaga tttggatcat taagcgtcct cttaagattt agttgtgcaa gctttgtctc
     3241 acatatttca atttttttag gctctggaag tttttcaaag ggtttctttc ctgatgtctc
     3301 ttcctcactc ccttcttctt cctcctcttt atttacagcc ttagaacctt ctgttgtttt
     3361 cgtgatttta taaccagcat ctgtagcagc tttctttaaa aaggaaagtt tggtcatcaa
     3421 agcggagggg acaagttcac cttcctcaag gaaaagcgtc gtggcacgat cttgaagtgc
     3481 tagttttata tcctttgggc acttattttc agtgagtttt ttcatcaaag caggcacttg
     3541 cttgagcgtt gtttcgtcga tgtttgcttc aagctcttca catagtagtg cttgttttct
     3601 tttcttctca gcctccattt ttttattttg gatatcgata tcaatatact tgctaccgat
     3661 tttagcgcct gcttttgcgc caatttttgc gagctctgaa cctgt
//
LOCUS       DAIAHG010000117         3701 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_117_length_3701_cov_2.762417, whole genome shotgun sequence.
ACCESSION   DAIAHG010000117 DAIAHG010000000
VERSION     DAIAHG010000117.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117_length_3701_cov_2.762417"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1035 a          875 c          733 g         1058 t
ORIGIN      
        1 gccgcgactt agcaacactt tctccagcag aattgcctta ttttcgtcag cgcatgggcc
       61 ttgtttttca agattgccgg ttgattgaca cccttaatat ccttgaaaat gtatgcctga
      121 gtttgcgcat tttaggcact caggcaaaac gtgccaaaat ttatgctcat gaactcctcg
      181 aatgggtggg gcttgcaaca catctgtatg agtaccctca tgcactctct gatggtcaaa
      241 aacaacgtgt agccatcgca cgtgcagtga ttacacgccc tcttttattg cttgccgatg
      301 agcccacagg aaatgtggat gaaaatgcgg cgtttaagtt gatgtatctt tttgaggaac
      361 tgaataaaat gggtacgaca gtcgttttag ccacgcataa tcgctcgatt gtcaatgaat
      421 ttgcctatcc agagctcatg cttgagcgcg gtcatttgca gctgatgagt ggaggtgacc
      481 ggagtgcttt ctagtaaaaa atcactgatt cttgtggatg agcattcgaa caaacctatt
      541 cgtcttatca tcggatgcct tgtttttgtg ctgacgcttg tcatttcagg cacactggcg
      601 ctgaatcatt tcgttgaaaa attcacctca gatctcacct ctaatattgt ttttgaagtg
      661 aatttgacag gcattgaacc gcttcaacag ccacaacttt tagcatcact acagcaagtt
      721 attaatggtt tcccctccgt gaaagaaagc ttcgttgtcc ccgtcgccag ttcccccata
      781 gccttacacg aaacagccgc accccttgct tttattgaag ctgtcattca cgaaaagcca
      841 ccgcttcctc tgggtgaagt gcttttaaaa atgcaacaaa ttcatcccca agttcatatt
      901 caatctcacg cctttatgac agcgaatatt cgttacttgt acgagtggtt aaagcttttc
      961 tcctatggga tcattgcgct gatctccttt gtgattatta ttactattag tgtgattacc
     1021 cgctccggtt ttcagatgca aaaaaatgtc gtggatattt taaggcttat cggtgcaccc
     1081 agtacctata ttgcgaaaca atttcattgg atagcctttc gtttaggttt ttcctcgagc
     1141 tttatcggtg tttttttcgg tgttgctgtg tttttcatga cagcctattt tggcacaaat
     1201 tttggcctgc caaaggatct tacactctta aaccaaaatc ttatcattgc ctttgttctc
     1261 ttgcccttta tcgtagggtt tatcagcctc ttcgtcgcaa gatttgaagt gatgcggaca
     1321 ctgattaagc tggacaaata actgattagt gtagattttg ttgtaagtac tacaactata
     1381 acttcggtag tgtgacgcct ttttgcccca tatattttcc atggcgatct ttataagatg
     1441 tctcgcacag agaatctgcc ttaagaaaaa gaaactgaca ggccccttca ttcgcataga
     1501 ttttagcggg caggggggtt gtgttagaaa attctaatgt cacatgcccc tcccactctg
     1561 gctcaagagg tgtcacattc acaataatac cacagcgtgc atacgtggac ttcccaagac
     1621 aaatcacaag cacatctttg ggaatgcgaa aatattctac ggtacgcccc aaaacaaagc
     1681 tattgggggg aataatacac acatcggttt ctctatttac aaagctctgc tcagaaaatt
     1741 ctttgggatc aacaatcgca ttatccacgt tggtgaaaat tttaaactcc cgagagacgc
     1801 gcgcgtcata cccataggac gacacaccat aggacacaac tcctttgcgc tcttggtgct
     1861 caacaaaggg ttcaatcatc cctttttgag cctgtgcgcg aatccaatga tcgggaagaa
     1921 tagacataaa aacgtaacct taagaaaaag aatgacctta ctatactgaa aaaaagatat
     1981 ttatcctagt cttagtcgct ggatttgtga ggaagatggc atagatattg caggatgaaa
     2041 ggatagcgct ttttagatgt atcaaaaaat gttgccttgg ctttcgagtt tgtttttggg
     2101 gttgattgta tccgcctgta acccctttaa aaacatggcc cctgactcca cagatattca
     2161 aacgcgcctc ctcgccaaaa ggggggagat ccctgatccc tatcctcttt attgttatca
     2221 aactcttggc gagaaaatgt gctatgaaaa accccttaaa aacggagacc cccgccttgc
     2281 tggctattac ggcccaccac cacggcaata tgagtaacat tatttttgaa taatggcctt
     2341 aacatagtgt gatctcaaaa gctatcttta gtaatattca cccacgcgta aaatttccca
     2401 atcaagggtg acacccgatg tttctaaaac acgctgacgg actttatcac caagatcctc
     2461 aagatctttg gcggaagcat tccctaaatt catcataaaa ttacaatgct tttcagagat
     2521 cacagcatcc ccttgcatca acccccgaca tccagcctca tcaataagtt tccacgcaga
     2581 gtcattttca gggtttttaa aagtactccc tcctgttcgt cccttcacag gttgggtttc
     2641 ctgacgtctt tcgagaatat cttgcatacg cctttgaatc tcttcttgag gcgcttgtgt
     2701 tgtgtaaaaa gcagcctttg taaaaatcca atcctttggc acagaacatt tacgatagcc
     2761 aaaacccatc tcatcggcag aaagacggtg aatctcgcca ttgggtgtaa cagcttccgc
     2821 ccacaaaagt ctatcttttg tttccacacc ataagccccc gcattcatgg ccagagcgcc
     2881 cccaatcgtt ccaggaatac taataaaaaa ctcaagacca ctcagcccat gagactggca
     2941 tgtaagcgca agcgtgcgat ccaagcagcc tgcgcctact tcaacgatat gatctttgac
     3001 aacaatttgt gagaaatccc cgattaaacg gagtgtaatc cctcgaatac ctccatcacg
     3061 aatgataata tttgagccag ctccaagtgt aaaaatggga agcgtgttgg atttgagaaa
     3121 aaaaataaga tcatcaagat cctttggctt aaaaaggata gatgccggcc ctcccacaga
     3181 aaaccatgta agacttttaa gcaagacatc atattggtaa cgcccacgca cctttggaag
     3241 atcaaaagaa agggtgtctg catccgacat agtttcaaaa ggaagcgcca tagattaact
     3301 cactgccttt aggctatcat tgtctgaact gtccaccccc agctgatcca actgaacagg
     3361 gagagacgct gcccaatacg taatcgatcc tgcgcccatg caaataacta aatcaccgga
     3421 ttgcgagaga cgccttatca taaaaggaag ttctttgggg tcagaaatct catagacctc
     3481 ttggcctctt tctcgcattt tatgcgctaa aatttcagaa ttaatgcctt caatagaatc
     3541 ctctcccgca gagaaaacag gtgccagaat aattttatct gcatcaatca tcgattctga
     3601 aaaatcatta aaaaaagcct tgaggcgtgt atagcgatgg ggttgaagaa cagcgatcac
     3661 acggcctttt gttgcctgac gcgctgcctt tagtgttgtt t
//
LOCUS       DAIAHG010000118         3684 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_118_length_3684_cov_3.294705, whole genome shotgun sequence.
ACCESSION   DAIAHG010000118 DAIAHG010000000
VERSION     DAIAHG010000118.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118_length_3684_cov_3.294705"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          987 a          747 c          807 g         1143 t
ORIGIN      
        1 catcactaat attaatcagg atgtgaccgc catgatgata ggctttgagg gtgatcgttc
       61 ccgtttcatt tttggctgct gttttccgca cctcaggtgt ttcaatgcca tggtctgccg
      121 agttgcggac catatgcaca aggggatctt taatcagttc gataacttgt cgatcaagct
      181 ctgtatccgc accaatctgc ttaagttgaa tttttttctg taaatccccc gcaatatcgc
      241 gaacaaggcg tggcaatttc gtccacgccg tttcaatggg ctgcatacgg gttttcataa
      301 tattttgctg aagatccgtt gtgaggtgac ttaagcgtgc gagaacatta ttttgtgtgt
      361 tgctcgtttc tgcttcttca ttttgttgca aaagttggtt gcgcgttaaa acaagctcgc
      421 ccaccagatt cattaaatta tcaatcacgt ctaaattaat acgaatgctt tgattggccg
      481 cgtgattgtt cgcagctgct gcattagctg gagctgtttc ttcttttgga ggattttttg
      541 gcacagaagc tgtttgttga ttttcaacag gtggaggagc tgtttcaagc ggtgtttcta
      601 agggaatgtc tgttgtttga gtgggctctg attcttgatg ctctataggc tcacctgagg
      661 aaatattttc ctcacctaaa aaagtattct ctgaaacagg tgattcttct ctctcagaat
      721 tctgcgtagc ctcaacacta ttttcctcta ggagctcttc cttcgtttca acatcatggg
      781 tatcatcatt agctttttcc ccttcctcaa gacataaatc caatgctttc agaagatcgc
      841 tgtcgcttcc ctcagattct tttcctttgg attcaatgga atcggcgatt tgacgaatag
      901 tatctaatgc cacaaaaaga gttgatgtaa taacaggtga agcgataagc ttgccatcgc
      961 gcattttgct aagaacattt tcagcaccat gggagacggc ttgcatgcgg gaaaagccta
     1021 aaaagccaca ggttccctta accgtatgaa gaatacgaaa agcatcccga ataagatcca
     1081 tattctcagg ctgagattcc aatgagagaa taatctgctc aagattttgc aaactttcat
     1141 aggtctctgt gagaaaatca gcagttaaat catccattgt cagatatcag gcacgttgag
     1201 catactctct tacagtgtat ttttataaag ttactaaaaa gttaatattg caaaaataga
     1261 acaaactatt ttcatacaaa gaaaggacgt gatataatct ttatattttc actatacaga
     1321 tttgaggata tttgttatga gctttatctc ttctttttca tcagggccta ctcggaaaaa
     1381 tcctcattgg tctttgaaga agcttgaggg agctattctt ggaaggtccc acaggtctga
     1441 tgtcgcgctg aataagctcg aggcgcttac ccttaaaaca cgcactcttt tagaggcgcc
     1501 ggaagatttt cattttgcga ttatgccggg ctctgccaca ggcgctattg aagcggccct
     1561 ttggcaactt ctggggcctc ggcatgttat cgcgcacagc attgatattt ttagtaatat
     1621 ttgggcaagc aacgtagtcg atgaacttaa aattccagca actgtttatc aaagtacgca
     1681 tgaacatttc ctggagcttg agcatgatcc agaaaaagat cttcttctca cgcttaatgg
     1741 aacaacatca gggataatgt atcctcacat ggactggatt aaaaaagacc gtgagggtct
     1801 tgtgatgatg gatctgacgt ctgccgcttt ttgcttgcca cttgattggg aaaaaattga
     1861 tgttgcagga ttttcatggc aaaagggtct tggcagtgag gcagcacacg gaatgttagc
     1921 tctcagtccc cgtgcacttg aaaggcttgc cgcctatacc ccctcttggc ctgtgccgcg
     1981 tcttttcaag cttaaaaaca atgataaaat taatcatggt gcttttaagg gggttacgct
     2041 taacacacct tcgatgctgt gtgtggagga ctatttagtt gctttagagt gggctgaaaa
     2101 tataggaggt tctgaggaat tatatcaaag aacccttgag aactttagta tcatcgaaaa
     2161 ttttataaaa aattcttctc tttttgcatt ttcaacggag cagtatgact gcgtgtctcg
     2221 ggtgtcgccc tgcctcatca ataaaaatct tgcggaccta aatttacctg agaaatgggc
     2281 gtggtataaa aggattggaa aaatgatcga agatgagggg ctggcctatg attttgtgaa
     2341 tcatgcgctc agtattcctg cttttcgcct gtggtgtggg cccaccatga atccaaacga
     2401 tatcaaaaaa ctcctcgagc gtatccatat agaggcttta agagattaga ttttaaattt
     2461 tattcatact tagaccgcat ctgtttttct taaaaaaatg agaggatctg cgaggataaa
     2521 gtgtggggtg agatccccaa atccaatcga taaaacaaat cctaaaaggg tgaaagtttg
     2581 tttaaaaatt ggtgaccaag ggggccattc atattatcgg tgaggtcacc acgtccttgg
     2641 taaccgatac ggagttgcgc gattttttgt gtcgtaatgg tattggatga gctaatatcc
     2701 tcacgccgga tcacaccttt gaggtaaata tcccgtacct cattcacaag gccaagttct
     2761 tgacgcccct cgatcaccat agagccattg ggaagaattt gaataactgt tgcggccata
     2821 ttaaatttaa gtttgtcttt tacggtataa cttcctgcac ctgtcatact tggattagac
     2881 tgagcattaa gaagattcgt taatgttcca ctcgtcgctg ctgcagctgg tgtaacaggg
     2941 gctataccca tattgcgttt gcgaatcccg tagatattcg atatacctgc actgaggata
     3001 gagcttttat tgattttagg cgtcatttct attttttgct cttgatccat atcaatggag
     3061 actgttaaaa tatcgccgac tttatttgct cgttgatctt taaagaacgc cttggaccct
     3121 gtttgccaaa gagagtttga acagggggtt acttgctcca ccggatcagg cataggcata
     3181 ctgacgggat gatattctgg aaggagtgtt ggatcttgaa tttgcgagag aggtggtgga
     3241 tcgccaatac gcgcaagctt ttctgctaaa ttacatccgc tcaaaaagaa aacaagggat
     3301 aaaaaagtcc atgcctttac atttttcatg aagcgacctg catttctacg gtatgtggat
     3361 caagtacagt ggcctcaatg attttttttg ctttccctgt tgtttctaaa gtaatgcgct
     3421 gcccaaaagc gccatcctta agggctttgg cctgcgttgc gatacttaaa tttcccgagc
     3481 ggtaaatcac gcggaccaaa ccatcccgtc gaattataat aggtgccata agatcattta
     3541 tttgaagggg agcacgtgcc attaaaagct tgtgttttga ttgctttcca agaagatcat
     3601 ccgtacgtaa tgcataatgc ccgtgaagat tacctatagg aactttcatg gtagttatat
     3661 cttctgcttg aataatatga ctcg
//
LOCUS       DAIAHG010000119         3678 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_119_length_3678_cov_3.246320, whole genome shotgun sequence.
ACCESSION   DAIAHG010000119 DAIAHG010000000
VERSION     DAIAHG010000119.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119_length_3678_cov_3.246320"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1004 a          653 c          723 g         1298 t
ORIGIN      
        1 ggcccgacaa gtgcgaactg cagccccttg cgtctttatt ttataaaatc aaaggaggcg
       61 aaggaaaagt taaaaccatg tcttgctcca ggtaatgtgg aaaaaaccat ggcagcgcct
      121 gtaacggtta tttttgcgga tgactatgct ttttatgacc atttgcccaa gctttttccc
      181 catgcagatg ctaaatcttg gtttgtggga aaccaaccgc ttattgacac aacagccttt
      241 cgtaatagtg ttctccaggc tgcctatttt atgcttgccg cacgcttgca tggtcttgat
      301 tgtggcccta tgtcgggttt tgataatgca caggttgatg cggtcttttt aaaagatacg
      361 tcgtataaat ctaattttat ttgtaatctt ggttatggtg atgcatcgaa gctttttccg
      421 cgctctccac gctttgattt tagtgatgtt tgcaaggtct tatagagttt tatcttgaca
      481 caaaaatata aataagctaa agatcaagag gtctattggt ccccatcgtc tagaggccta
      541 ggacatcacc ctttcaaggt gaagacacgg gttcgactcc cgttggggat gccataatct
      601 tatggcctgt cttttctgtg tcgtttttta gttccttctt aaagaaatca gtgactgttt
      661 ctattttaaa tgtgtaaggt tgcccattca taacctctgg attgtatcct gttaaagtga
      721 ggataccctt tgatatgttt tttgaggggc ttagttcaaa agagagcgtc ttaaacgtta
      781 agttttcaag cattttagcg atatagtttt tttgcattgc atcaagattc ttatcactct
      841 caagcatctc ggatccttta taggatattt ttccaaaagg agatgttgat aaaaattttg
      901 catttttaaa gctgagacca tgctcttttg aaaattctat ttgaccaaaa ccatccattt
      961 ttcctgagcc ggaaagatcc ctaatcccta taaatgttaa tgcattggaa agttcaaaac
     1021 ttttaatatc aaaaataaag gaaggtgctt gtgtattttg aaaagtgtgt aatgtaagat
     1081 cccctccaag ggcttttgct atgcactgct tagcatgaag attctcctcc catttaaacg
     1141 tgagttgtgg ctgatcaaga tgtaaatcgg aaaagtgtat agactttgcg tggatgactt
     1201 gcgggacttc tgctcttaaa ggaaaaagtt ttagattaag tatcgtattt aatttttcaa
     1261 cagaataaag aggatcggtg aatgatgtat cgtaaatatt aatttttgca aatccctcct
     1321 tctctttttg cgtcaaaagc gttttaagat atgacaaaaa gtctagggcc caaggcttct
     1381 ctttttcgga taaaagagaa aaatgaaaag gaaagcgtgc gaaaatggaa aaactcccct
     1441 ccccagacat atttttaata gattggattt tctggtcatt aaatagcttt aaaagggagt
     1501 acacaggaat tttcggggag tcaatcgata gatcaagaga actagataaa tcaacatttt
     1561 tgactgtttg gagagagagt gtagcttttt catgattaaa gtagatcgca ccaaacccac
     1621 ttatatcaaa agaagcattt gcttttttaa cttcagtatg tgtttcaaat tttaccaaag
     1681 aatgataagg cgcgttattt ttttctaagg caaaggagat atcgtcacca ttaaatgtta
     1741 tatcgatatc attattatct ttttcatcaa gagacaatga gagttctttt gctaaaacat
     1801 gtgctgaatc aagatttgct gagaaatttt tcagatgtat atgaaaaggg gcattttctt
     1861 tgtcaaaccc tcgatgaatt ttaacatccg ttgtaaggtt ttttgagata aaacgcaaag
     1921 aaccgtgagc gccgaaacaa agagggctgt gcgtaaattt catttccaaa ggaataaaat
     1981 agtctttttg atggtcatct aagtttataa aaccttttat ctttagaaca tgaaaaggga
     2041 tttttggctc atcaagcgat tctttgttat gactaagatt aagaaaagat ttttcaatat
     2101 cctctgcttt gccgatatat tgaaatccct tgatatcaat cgcatcaaac gattgtgtaa
     2161 agaggctatg ccatgagaca ttcaatttaa tgaggtcggc tgctaccttt ttttgtgtat
     2221 cttttgaagc aaggcgtatg ttgtataggt ttatactcaa atcaggggct gataagcgta
     2281 ttttatcaag atggataagt cgaaaatcat agtgtgatac tgtgtgacga atgtgacgct
     2341 cgacaatttt ttcaaatcca atccaaagaa ttccaaaaaa aagaaatgca cctgctaaaa
     2401 agccaaaaag cattcgcact cttcgcatcg tattgagggg cattttctgc tactctctaa
     2461 agaaactgat aataagtgta gcgagatttt ttctatggaa acagcccctt taaagatgat
     2521 cagttttttg tggggtgttc cttttataag ttttttaatg gctcttgccg ttgttccgct
     2581 tttctttcaa gatttttggc acaagaatct tggaaaaatt gttgttgggt ttagtttact
     2641 gattattgtg ccgatgctct ttttcttaag ctcagaatct gctcaaaaga ttctttttca
     2701 tacactggcg cataattatc ttccttttac aattcttatt gcgacattgt ttattttatc
     2761 cggaggtatt catatcacaa caaaggggaa aataacgccg tttggcaatg ttttattcct
     2821 tttgcttggg agttttctgt cgagttttat ggggacaaca ggggcgtcaa tactcctcat
     2881 tcgccctttt ttaatgatga atggacagcg ttatcacaag cactaccttg tgattttttt
     2941 tatttttacc attagtaata ttggcgggtg cttaagcccc attggcgatc cgcctctttt
     3001 tcttgggttt ttgaatggta taagtttttt ttggccgttt aagtgtcttt ttcaaccgtt
     3061 tgtctatgta atgtttttcc tcctttgtgt tttttatatg atcgatcgct tctattgtcg
     3121 tggtaaggat tttagggatg aggtaattga tcttcctgag aaggttaaaa tttatgggaa
     3181 acgtaatttt ttagggatta ttctcgttat ttgtgcgctg attttttcta gccttgatat
     3241 cccctacatc catcttatga ccaatgtcat gcttattctt attgccgtgg cttcttgggt
     3301 tttgacgcct aaaatggtcc atcatatgaa tcgctttagt tttgaaccat tgcgcgaggt
     3361 gagccttgtt tttcttgcta tttttattac ccttattccc atagaagcta tgctccacct
     3421 tggaaaagag gggcctcttc atatgattgc aacacttgct aatcctcaaa actatccaga
     3481 tcctctgcgg tattattttc tgacaggaat tctttcaagc tttcttgata atgcgcctac
     3541 ctatttgctt ttttttcata tgctcgatgg cattatcggt ggagggcctg cagcattgat
     3601 gacgacacac gcacttacac tcaccgcatt gtctttaggg tccgtttttt ttggcgcatt
     3661 gacctatatc ggcaatgc
//
LOCUS       DAIAHG010000120         3652 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_120_length_3652_cov_3.615944, whole genome shotgun sequence.
ACCESSION   DAIAHG010000120 DAIAHG010000000
VERSION     DAIAHG010000120.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120_length_3652_cov_3.615944"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1178 a          670 c          780 g         1024 t
ORIGIN      
        1 ttttcatgtg agctatggta tagtgcaaac ttccacgatg aagggttact tggggggcga
       61 attgtggccc ttttgccttt aggatttgcg aaccctcagt gatacggggc acttttacct
      121 tagcccaaat atgatacaca tgggcttgat agcgctttgt cgcctctgaa caacctgatt
      181 tataggcctc aacagcgcga ggccacgagc catattgttt gtatagtcga gcgaggaggg
      241 ccgctgcaaa atcaatattt ttcttgatat ctaacatatc gtcaatggat ttaaagcgct
      301 ttatgtgcga tggtaagttg agctgcatgt atccaatatt catattcttc accccttggg
      361 cacgaagagt gttcacaaat tgcacagctt tctcttttgt ggaaaaatag tatccgcgcc
      421 ccttggcatt aatcgcataa ggcgtgcatt tcgactccac atgggcaatg gctgtgagga
      481 ggttttctgg aattttatgg cgttgttctg cttcgcgcgc atggttaatc gcaggcaaaa
      541 tatgtttcat atttaggtaa agacctgcct caacaagagt gtgcgacact aaaaaaaata
      601 aaaatgcaaa aagaatacgc gttatcttca tcgtcttacg ctgctcttac ttcaaggact
      661 tctggaacat aataacgcag catattctca ataccggatt taagcgtggc actggagctt
      721 gggcagccag aacaggctcc ttggagtttc agatagacaa taccatcctc aaaacgctca
      781 aaactaatgt cacccccatc acttgccaca gcgggacgaa cgcgcgtttc aataatatcc
      841 ttgatctgac gaatcacagg gtctgtctcc tctgtcactg gattcgctgt tttttgtgcg
      901 tcatttgttt ggactgctag aaaacctgcg ccaaaatgct ccatcaaaag tgccataaca
      961 aaaggcttga ggtgacccca ttcaatgtca ctttctttag tgagtgtgat aaaatcctgg
     1021 gcaaaaaaaa cagaaataat accctcaatg tctaaaagct gtcgcgctag aggcgatcct
     1081 tcacagtcac tgtcttgatc aaaagacaaa acaccctttt ccaaaacaat acagccagga
     1141 ataaacttca ggctgtcggg attaggcgtt gtgtcggttt gtataaacat cgataaactt
     1201 taaggttgat aatatcaaag gcaagactaa caaaaaaaaa gtggaagatc aagttttttt
     1261 aattttatac tgtttttaga agaagttaag gataattaat atattcgtat aatgtttttt
     1321 tacttattaa tgtgcaatta aaaataatta catgcaaagt gcctattgaa tgaaaatatt
     1381 tttccgtgag ggcttgtttt tttgagaagg gaagaaatct ctaaaatctt cattaaccgt
     1441 taactttttc cccctacaat caagtcatgg aagtgatttt tttgattgat gtgcggtgaa
     1501 gttttttaag tcacgctctg aagacaaatc ctttttaaag ggctatttaa cggggcaaat
     1561 tctcattgct tcaccgttta tgagtgatac gcgttttcac ggggctgtcg tttatatatg
     1621 tggccacgat gctcaggggg ctatgggctt tattataaac aaaccactgc caagtattgg
     1681 gttttatgat cttctcgatc aacttgatat cgaccatgcg cttgaaggta tgagggagtt
     1741 ccctgttttt tacggggggc ccgtcgaggt ggggcgcgga tttgtgttgc actcaagtga
     1801 ttataatcat ccctctaccg tcaaaattaa tgatgatttt tccatcactg caacgcttga
     1861 gatccttcag gtgattgtga atcagcttgg gcccaagcgc tctcttttga cgcttggata
     1921 taccggttgg tcggaggggc agcttgagca agaactccaa gaagatcagt ggtatgttct
     1981 cagcggcaca aaagattttg tttatgaaac gcccgccgag gtgatgtggg atacggccta
     2041 tgagaagctt gtgattgatc ctcacgcgat ttcaatggag tcggggagtg cttaggaaaa
     2101 attaactaaa aatatattga tatttgatta aagctatggt tacaattgat ttttaattaa
     2161 ttttaacgaa gttttatgaa aaaatatatt ttaatgttat ctatcgtcac tgtgacaagc
     2221 gctttcgcat cgaatacaca gaagcacaaa aaaattgaaa tctatcgtga tccaggtcta
     2281 gagagttcgg aaaagaaaac gaatcttgct caaacaataa aagaggagaa aacaaccttt
     2341 aaaaatccat tttatgttgg atcaaaaatc aaaaaacaaa agaaaactga aacaagggag
     2401 cctttcaaaa taattaccaa aaaggataaa aaaattgaaa cgcttgagga aatcaaaaca
     2461 aaatacacaa tgattaataa gcttaatatt ttcattaatc gatatgattg tttaacaaag
     2521 tataatgatg agaaaaagga taatagtgca aaaatagtga agttaaagga agacgttgcg
     2581 gaatacattt ataaaaattt tgagaacgtc aaagaactga aaaaagaaag ttctaaggtt
     2641 tatgaaactt acaaagtaga tatttacaac cttttgtatg aactttatca gcctgagatg
     2701 acgctcgaag agtttcaaac gaatgttttt agcacgctaa agagtgggga tcgtacgaca
     2761 atttattttt ctgaggatcc taatgattct aataaaaaaa ttctttatga gactccttta
     2821 tcccttgtaa aactttttaa agaataaatg ttaggcaacc tcctgctctt ttctatgctt
     2881 gagaggagca gtttttcgct caagttttat gacatagtaa aatcctggaa cagcgacaat
     2941 acagctcata aaaaagatat acgtccaaga aatatgatcc gcgatatagg cggcacctga
     3001 ggaggtgaga acgcgacaga gcgatccaaa agaataaaga atcgtaaagt gtgtggctgt
     3061 gtgcggctgc atgcaaaact ttgagaggta ggcgataaaa acggcggatg tgaggcctga
     3121 tgtgaagctt tcaacgccca ccgtaataat gagagccgtg atgtcataac cgataatggc
     3181 ttgaagtgtg aacatgaggg ctgaggcaat ttgtaaagaa aaagcgagga taacgccctc
     3241 aagatcccct aaaaacttaa gaagaatacc tcctgagagg gcgcccaaga ccattaaaat
     3301 cacaccaaaa acctttgaaa tatctgcaaa ttcaaccttg ctgacgccaa gttcaaagaa
     3361 aaagggcgca ctcatggcgc ttaaaactgt atcaatgcat ttaaaacaaa aaataaaaag
     3421 aaggatatga aaaaaatatt tttgctgaaa gagagattga aagggggcct tgaacgttat
     3481 gcgggaaggt ttgatcacaa gatgggggag tgtttgcgtt aaaggcgttt ttaaaatagc
     3541 aatgatgccc aaaaacatgc atcctgccat aattgtataa gcccattgcc aactgagggc
     3601 tgcggctatg taaagggcgc ctgcgccgga cgtcaacata ccaatacgaa ac
//
LOCUS       DAIAHG010000121         3612 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_121_length_3612_cov_3.447808, whole genome shotgun sequence.
ACCESSION   DAIAHG010000121 DAIAHG010000000
VERSION     DAIAHG010000121.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121_length_3612_cov_3.447808"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1141 a          679 c          733 g         1059 t
ORIGIN      
        1 ctccaagcat cagtgctgtg ccagaaggcg catcaatttt atcacggtgg tgtgcttcaa
       61 aaatttcaat atcataggca tcatccagga tttttgcggc atgctctaca aaaaaatgca
      121 taagcgcgat cgttggattc gtgttaggag ctaggcaaat aggaatgaac tgtgcggcct
      181 ctaaaagaga gtgatgttga tggggctcaa gccctgtcac acaataaaca agaggctttc
      241 tgtgatgtat agcatgaggg agataatcaa ccagcgcctc cttgcgactg acatcaatca
      301 taacatcaca gtgttctaaa aaagtatcaa tcgctgtggt cgcaaggggg tgatcttttt
      361 tttcgcgtac cttgatcgac gagcattgcc gctctttttc ttgacgcagg catgcctcga
      421 tcgcttgccc catgcgacca aaacccataa ttccaatgtt taacactaat gatgactcgt
      481 ttttttataa ggttgttcgc tatttttgac gaaagtgcgg gaaagatcaa tccatcaata
      541 aggtttttcg taagaacagc gtgtatagat gatatggctc gtaaatgtat tgaggattcc
      601 tgcaaaaaga aactgcagca tcataatgac accttcgaga agaaaagtgg gcccaaaatc
      661 ttttattaaa aaaacaaggc caacgacaac gcatgtgttc aaaacaataa gactaagata
      721 gataatcagc agctgaaaat gaatgggcaa aacaatattt ttcacatcct ttaaaacaga
      781 gctcccgtca aatcccattg ccacaagacc tagaaaaaca aagataaagg gcatacacca
      841 aaaaaacaaa cctgtgagga aaagtccccc aaaggagagt cctatcatac ccaggcgtgc
      901 tgtaataatg ctcgtgatca gcccaagaaa aaggactgtc ataccgataa aaattgtata
      961 aaaaagatac acaagctcac tttttgaaaa acgcaaaaaa ggggcctctt cttgaaatgc
     1021 taggctcctg taccatgcca cactcacagc cgacataata aaaagggaac ctatttgagc
     1081 aatcagtggg ttcaaaagat gcaaagtgag tgaaacgata aaccatgcag aaatcacacg
     1141 taaatactgc agcgtcagtg tgcggtaaaa tccttctctt aaagattcaa cagccgaaaa
     1201 accactgata tttgcgaaaa cagtgatgcc ggtttttttt tcatgcgtca taaaaatcgt
     1261 gcttgtgctt tttttatggc cctataatag cactgtttta actttttaga gtgtaaattt
     1321 cacagaatct gtgggggaat catctaccga gctatcatca ttttcttgat aacgtttgag
     1381 acgatccttg agtatatttt caagcaatac atgggaaatg gctgttgttt gtgttgtaac
     1441 agattgtttg ggaagaaccg cttcaaaaga acttacaacg ccacgcatcg taaggggttt
     1501 gtcgatgcta tagatggtta gaaaatcaac aggggggtta ggctctctaa aaatataact
     1561 ttttttatcc actatttctt cttgagcgag aggaaaagaa acttctgcag atgaagaagg
     1621 taataaatga ccctcaaaaa tctttttttc aaggtcacca agggtttcca agcaatcttt
     1681 cacagaacag tgtccacctt tttcattata aataaggaca aaaataaagg tattcacaag
     1741 gctttcttgg taaacttcta tgcctttcag cgcctcaggg atgtcgaaga taaggatttt
     1801 aggcccatcg atatttttaa aaatgcggga tatttcatag agattggtgt gaaaatgaat
     1861 cttatcctga cccaaaatat gcatgatatg ggggagagat ttacgcaaaa gtgcatcatg
     1921 tgtaataagc ccaatggtat actcatgaag tgaaatcatc taaccacaca ccttttttat
     1981 ggatcttatc catagtgtgt cgaataaagt ttaatagagt cttaaaaaaa gaaatttttt
     2041 tctataatat aaaaaaaccc tctaaaaaaa cttagagggt ttgatacaag aattttaatt
     2101 tgaaaaacta aaattagcct tgacgtgctt taaaacgagg tgcctgctta ttaataacat
     2161 aaacacggcc acgacggcgc acaactttgc aggccttatg gcgacttttg agggtcttaa
     2221 gtgagtttgc gattttcata atacaaaact tttcttttct aaatgtgggg ccattatgca
     2281 gtctaaaaac aaaaaaagca agaaaattta tgcaagcgaa atcacccgtg gtatttaaaa
     2341 gtctttcgtg ataaaaatag taaggtaagc cttggagagt tatcatgaaa agcgttctga
     2401 tcattgacat taaaaccgac aatagtgctt ttgagtctat tgtccaaaaa agcattcata
     2461 ttttagacga gcaaggtgtt acttattcta aaatctgtgt caatcatatc cttgaagcgc
     2521 ctattgcttt gcgttttgcg attgaaactt ttagatccaa tcatgacaag aacagcaaaa
     2581 gacctgatgg ctatatggtt tttggttttg tttcaaaatc agaagacttg gaaaccgttg
     2641 tgtatgaaga atttttacgc tcattgcaag acatggcgtg ctattatggc ttagccctgt
     2701 cccatcacac gcattttgaa tctcaatcct cacctaaaaa tagtgatagc atcgcccaac
     2761 agattgttta ctcttgtctt gatcttattc agctcaaaag gcagctgggc cttcagtcaa
     2821 gccatatcaa taccccctaa gagttcataa cttaacttac tcaacttatt gcttaagatc
     2881 attgaaagac atatgacaaa gaaaaaattt acaaaacgtc gcgctgcacg gttttttatt
     2941 acacaagctc tctatcaaat agagcactcg gaccagcctc ttcccctttt aatagggcaa
     3001 ttcgttcgga ctcacttcga gaatagtgat catgaactcg caggtgatgc ggatgaagat
     3061 ctttttcgtc gtattcttga gggtgtcaca catcatcaaa gtgagattga tcagacgatc
     3121 ggtcgtatcc ttttggatgg ctggacggta gaccggttag atgctgttgt gcgtgctctt
     3181 cttcgcgcga gtacgtttga aagcatgtat gatcttgaga cgcctttgcc cgtcattatt
     3241 aacgagtatg ttgaaattgc taaagttttt gtggctcaag gggaagtgac gtttattcat
     3301 gtagctatgg ataaaattgg ccatttggtg cgagaggcta aagagaacaa aaattaaaaa
     3361 agagtgtatt ttttgcgtga ttaggccatt tttggggtga attttcgcgt tcttggccgc
     3421 tattttgtgg tttttcaggc aaaaaagggc ttttttaacg catcatgcac gataataggt
     3481 gaatttttag gattttaacg cgaaatttcc tcgatggtct cgcgacggct gcttgaaaag
     3541 agggggtaag ttgtattttt gcgaagattt tttttaaaaa tttctcttct cagtgcgccc
     3601 ctagattttc ct
//
LOCUS       DAIAHG010000122         3608 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_122_length_3608_cov_2.878505, whole genome shotgun sequence.
ACCESSION   DAIAHG010000122 DAIAHG010000000
VERSION     DAIAHG010000122.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122_length_3608_cov_2.878505"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          976 a          726 c          855 g         1051 t
ORIGIN      
        1 gataaatcac tgcgccgtaa agttcaagag gcgattatgg cgttgtggct tgagtggcat
       61 tttacaaaag aacagattct gacaatttat ctcaatcgcg tttatctagg ctcgggtgct
      121 tttggcgttg atgcggctgc gcacaaatat tttaattgtt cagcgcggca actgaatgtg
      181 tatcaagcgg ctgtgattgc aggcctttta aaagctcctt cgcgctattc tcctttaagc
      241 aatccggaaa aagcttatga gcgtgccgcc gttgttctgc atcaaatggt tgaggcgggg
      301 tatatcgacc gtgcggatca ttatttggcg caagcaaagc aaaacttaat gaataacccc
      361 gatatcaagg ctcaaagcag cgtaaaattc tttactgatt ggatttatga tcaaatcccc
      421 agttatgtac cgattgatca agatcttgtg gtgataacaa cccttgatat tgccatgcaa
      481 cagcatgcag aaaagatttg tcaagatttt aaggctatgg gagaggagct taaggccagc
      541 gaaattgcgc ttgcttcgat gcgtgcagat ggcgctatta aggcaatgat cggcgggtat
      601 gattataaaa aaagtcaatt caatcgcgca gcacaagccc ttcgtcagcc aggttcgacc
      661 tttaaaatgt ttgtttattt agctgcctta gagcatggtt ttacacctga tacgatgata
      721 gaggatacgc ctgtgcaaat tggagcatgg gcaccaagta gttataaatg gcagtcacgc
      781 ggtgaaatta cactgagaga aggtttgacc cactctgtta atcctgtcac agtccgtgtg
      841 ggccaggctg taggtgctca taaaattatc gaggtagcgc accgccttgg aatcacaagt
      901 aaaatgacac ctgaccttag tattactctt ggagcgatgg atgtcacact ccttcaactg
      961 acggcatcct ttgcaacttt tgtgcatcat ggccttgcag tgtggcccta tggcatttta
     1021 gaaatacgca caaaagatgg aagagtcctt tataaacgag agcctggggc ccctgtgcgc
     1081 gttgtggcaa aagaacaact cgcaggcatt cgtgatatgc tccattctgt ggtgaaactg
     1141 ggcacagggc gtgcggcaaa atccgctgta ggcggtaaaa gtggaagcaa cggcgacagg
     1201 gatgcttggt ttattgggta cacagataga cttgtaacag gtgtttggat tggcaatgac
     1261 aataacagtc ccatggtgaa aagatctgtc ggtgggcgta tgcctgcaag ggtgtgggag
     1321 gcatatcatg caggtctcaa tgcatctcaa ggagaggaga gagatgtgga taatcttatt
     1381 gatgagatcg cagctttggg ggatgcaccc attattgagg gaagggctgt gacagaggct
     1441 gcagcgcgtg cagaggctgc ggccatcgcg cgccaggcgg atatcccggc aagtgagccc
     1501 acagcccaag agcttgaaca ggataatgtt gctgagcttg cgggtgtttt gggtcgcagt
     1561 cattaatata tagcgaaacc attgcatatc atcatctaaa aatgaactcc tttacgaacg
     1621 ctgagatttt acgttagagt aaatatattt cttttgtttt agagatttct tctatgacgt
     1681 ttgtttccag gtctttcgtt aaaatgatcc tttttataag tcttgtaggg acgggcgtag
     1741 ggctattgta cacacctctt tttaatgctt ttagccataa tcccatcttg aacacgattg
     1801 tttttagcgt tttttttgtg ggtattttta ttaattttct caatctctat cgtgtaggaa
     1861 aagagcaaaa atggcttgaa tcctatgacc gcggtcatga acgttttcct ggaacacctg
     1921 accctaagat tcttgcaccg gtggctctga ttttaaacga atcgaaaaat caatatctct
     1981 ctccgatgct gattcgctca ctccttgcca gtgtggaggc acgcttggat gccatgcgtg
     2041 atgtgagccg ttatattgtg gggcttttga tttttatggg tttgcttgga acgttttggg
     2101 ggctttctca aacaattggc gccattgcag gtgttatttc aggtattgat cttggcgctg
     2161 gtgaggttaa agatgctttt gcaaacctaa aagagggcct gcaatccccc cttaacggca
     2221 tgggaacggc ttttagttgc tcgatgtttg gcctagccgg atcccttatt gtgggctttt
     2281 tagatttgca aatctcgcgt ctatcgacgg atttttatca tatggtcgag gagcggttaa
     2341 cacttttaac aaaaattaca acagcagaga tcagtgagcc tcattcaagc ggtcctgcct
     2401 atacccaaag tcttttagag caaacagcgg aaaatatgaa tcagcttcaa aatcttattc
     2461 gccggggcga ggacaatcgc acaagtgtga ttaaatcaat gcaaggtgtg ggtgaaaaac
     2521 ttactgtgtt gacggatcaa atgatgacaa atcaacagct tctaaagaaa attgctgaaa
     2581 atcagatcga tctccaaggg gcattgcaac aatttcatcg cactcaaaat ggcgggggcg
     2641 aagatatcct caagcatcat ctgcgcagta tggatgccac tcttacaaaa cttttagagg
     2701 aaagcatcga agggcgcaat cgcggcatcc aagatgtccg cggcgagatt cgccttgtgg
     2761 cgcgcacaat ctccgccctt gcaaatggtc aggaagttgc tgcataaaaa attatattct
     2821 tggcaaaaag ttcttgcgta aaaatttttc ccctagagct ctttatcttt atattcacat
     2881 agtttttcta aaaagcattt ttgacaaagg ggcttgcgag ctttacagat ataacgtcca
     2941 tgcaaaataa gccaatgatg tgcgtgatat aaagcctctt taggaataac ttttagcaac
     3001 gcttgttccg tatccaaagg tgttttcgtc gcacaaagcc ccaaacgatt gctcacacga
     3061 tggatatgtg tgtctacggc aagcgtaggc tcgtgaaagg ctacattaag aacaacattt
     3121 gctgtcttac ggccaacacc cggcaatgac tcaagggacg ctcggtcttt gggtacagag
     3181 ccgtgacaat tttccacaag cgcatgggag atccctaaga tatttttggc tttcgtttta
     3241 aaaaaattaa gtgttttaat ggcttcttcg atctcttcaa gagacagttg caacattttt
     3301 tcgggtgttt tagccctctt aaaaagcttt tctgtggctt tgttcacatt catatctgtt
     3361 gtttgcgcag agagcatcac cgcaatcaaa agagtatagg gatcctcgta caccagctct
     3421 gtttcaggtg taggattgta tttttcaaaa agctttttgt caggttcatt ggtattaaat
     3481 ggaacatatg gaattctcat tttttttctt aaatcgaaca tctgaatttt gtcataaatt
     3541 aataaagtat tgtccaatcc tttttgaaca acatcgtatc ctaaatattg atccatataa
     3601 gaatgaat
//
LOCUS       DAIAHG010000123         3580 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_123_length_3580_cov_3.239509, whole genome shotgun sequence.
ACCESSION   DAIAHG010000123 DAIAHG010000000
VERSION     DAIAHG010000123.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123_length_3580_cov_3.239509"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1130 a          725 c          735 g          990 t
ORIGIN      
        1 aggggggacg tttaacagga attttaaggg tttttttgca aaatagtgaa gaaaaaggct
       61 caaaaatctg aaatttcatg aagatattcc gaaaaaatgg tgcgattatg cttgaacgag
      121 gcaaaaatac aactcttttc gtgaaaaaat cgctcaaata atatgcaaac gggcataaaa
      181 atgaaaaatt tcgtgtcaaa gcagaaaaat tattccgaaa atcgcatcaa tgatacgaaa
      241 aagcttcaaa acggtgcaat tgttgatcaa aatataaaaa aaacgcttct gagcttttta
      301 aaaaagaaaa aatcgctttt ttcataggat tcagcgtatt tttccaacaa tttagcccca
      361 aagaggtaaa acaccacgaa aatcgcctca atgatggtaa aaaaagtggt aaaaaccact
      421 tttttggtga aaaaatccct caaatatgag gcaattatcc tcaaaaaagc tccataaaac
      481 ctttataaaa agaaaaaaca acagcttttt cactaaatca aaggtttttc tgggaaataa
      541 agcggtgtgt ttcatcggaa aaagtgaccc tttttcaccc attttttcac caagttttta
      601 cattttctac cccataaagg gtatattttc atgattttca tgcacattta ttataaaaaa
      661 ggatcaaaat atgccgaaaa aagggttttc acccacaatt atggttcagg ggaagcaaaa
      721 atccttcaaa aagagaaaaa tcacgcagtt ttctcgcctt ttttcctgat ttagtgtttt
      781 ttgattctat agtatcataa agacttaaaa acgaagaaat gcgcatcttt atgatgaaaa
      841 gcgctctaac catccctcaa aacgggataa tcataaaacc ccttgcccgt tttccgcccc
      901 agttgacccg cattcacata cgtgtagaga agggggcacg ggcggtattt atcctcccca
      961 aggcgctcat ggagatcttt taaaattgca aggcatgtgt caaggccaat aaaatctgcc
     1021 aatgtcagcg ggcccatagg atgatgcgcc ccaagggtca tggcgcagtc aatatcttcc
     1081 ttggacgcaa taccgctctc aagaacaaaa attgcctcgt taatcatggg aatcaaaata
     1141 cgattgagcg caaagccagg gtaatcctta actgtaatgg cttttttgcc aagggtttca
     1201 acaaaaccta tggtggtttc ataggtcgcg ggatttgttt taaggccgca aataatctcc
     1261 acaagaggca ttttcggggc agggttcata aaatgaatgc ctataaaatt ttcttccaat
     1321 gccgtaaaag aagcaagttc tgtaatagaa tagctggagg tatttgaggc gagaatcgtt
     1381 ttaggatatt tttctgttag ggcttttgtc gtgtgccaaa aagccttttt aatatcttta
     1441 ttttctgcaa tggcctcaat cacaatgagt gttggctcaa gacgtagttc atgaccaatt
     1501 gtgagagcgt taaaatgctc tttttcaagt ttttttgaaa gggtttcttt tgctttttca
     1561 cgttgagtct cactcatatc cacaaggtgc acaggataac ccgaagctaa gcatacttgc
     1621 gcaatccctg agcccatttg cccagcgcct ataatttgaa tgagtttttc catgatttag
     1681 actttctctt ttgcaggaag agggccaagc acacgtgtga cctcttcttt ggttgtgatg
     1741 tttttctgta ttttaagaag gccatcatcc cataatgtga aaggatgtgc ctttttcaaa
     1801 agacgtgtgg aggcattaga ggcaataaga tcacgggtcg tgtcatccag gacaaaacac
     1861 tgacttaagc atgtgcgtcc tttaaatcca ctatgttgac aggtatcaca cccagtacca
     1921 ttacacaggc cacatactgt tcgcacaagg cgctgggaaa ccatacaaag taaatgatcc
     1981 gccaaaagat cagcactaat cccaagattg acaagacgat gtgtggctga aaacacatca
     2041 tgcgtgtgga gcgtcgacag aacaagatgc cctgtcatag cagcgcgcaa agccattttt
     2101 gctgtgtctt catcgcggat ttcaccaatc agaatcacat ctggatcctg gcgcaaaaga
     2161 gagcgcacac cttgtgcaaa cgtcatctgg tttgtaattt ctgattgacg aattcctgta
     2221 atagcatatt caatcggctg ctcaagcgtc ataatattgc gtgtttgcgc atccatctcc
     2281 gcgagcaatg cataaaggct tgttgttttt ccagagcctg tgggccctgt gaataaaata
     2341 agaccatgat tatgctgaat gcattgacgc aattgctcct tgtggtaatc attaaagcca
     2401 agcgcatcaa gactgaggag cgattttttc ttatcaagaa tacgaatgac aatattttcc
     2461 ccataaacag tgggatgcga cgatacgcga aaatcaattg ttcgaccggc aaaggatttt
     2521 ttaaagcggc cgctttgagg gcttctttcc tcggccacat ccatagaagc caagactttt
     2581 atatgagtgc gaagagtggg ccattgttct tttggtaaat cgtaagctgt tcttaaaaga
     2641 ccatcaatac gatagcgaat tgcaatcgta ttttctgtcg gttggcaatg aatatcagac
     2701 gcaccctctc taagcgcttt gatggtaatc gcttcaaaat caataacaga ttcatgggag
     2761 ggcattgtgc cagaagagtg ttcataaaac tccaaaagct catccaaaag tttttgagca
     2821 atgtgataag gaataatctt tgtgtgatag tggcgctgaa gctgatcaat aagcaataaa
     2881 ttctcagggt ccatcatggc aacatgaaga cccgcttcgt ctttataaaa aggacaggat
     2941 ccgtgtgttt tgacggtgtg gctgtcaatt ttgatttctt tcatggggtg aggggaccct
     3001 ggaaaaacag gaatccctga ataggaggat aaaacacgaa gaaggtcgtt ttcctctaaa
     3061 aaaccttggt cacacaaaat ctgtccaagt ttcagatgag gttttttttg ctgaatttta
     3121 agggccgtgc gaagctgatc ttccctgaca aggttaacac ttaaaagatg ttggcaaaaa
     3181 cgatcccttg acacatttta tacccttggc agtgtttcaa aacaatgctc aggcgcaggc
     3241 gagggaagag tccactcgag tgttgtggca cttggacccc aaggattgcc tgcgcatttt
     3301 ttcttatccc aaaagacttt gaacactatg attaaaaaaa gcagcgtgga gagtaacaca
     3361 acagcaccac cgctggaggc gacaacattc caaccatgat aagcatccgg ataatcagga
     3421 atacgtcgtg gcattcccgc aagccctaaa aagtgcatgg gaaagaaaag aacattcaca
     3481 ccaataaaaa acatccaaaa atggagtttt cccagtgttt cattgtattg atagcctgac
     3541 attttaccga tccagtaata aaatcctgca aaaattgcca
//
LOCUS       DAIAHG010000124         3578 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_124_length_3578_cov_2.637818, whole genome shotgun sequence.
ACCESSION   DAIAHG010000124 DAIAHG010000000
VERSION     DAIAHG010000124.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124_length_3578_cov_2.637818"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1068 a          748 c          711 g         1051 t
ORIGIN      
        1 tcataagaaa gatggtcctc cccaggaatt ttggacttat tttgataaag atggaaaagt
       61 tgtctcaaaa gaggtcaacg aattccttgt ctatgatccc cgtaaacgtc cttggtacat
      121 aagcacagat caaaaacgac gttttaactg ggcgcctgtt tatattttta gttttcttga
      181 gataccaggc atcacggctg caacgcccct ttttcaaaaa gatgatcacc aagaaaaaat
      241 gattggtgtt gtgggaattg acgtgtcatt aaaggaaatt tctaaatttt tagagcaaaa
      301 tattatttca caaaacgaaa taagttttat tttgaatcaa aatgaacagc ttgtcgcatc
      361 ctcagatcct caaaacatga ttcctaaaaa ttctaatgat gaaattcaac atatttcaaa
      421 aatagaagtt aaaagcctca aagatgctta tgaactttat aaaaaaaata ataatcctct
      481 ttttaaattt agaaatgaaa aaacatctta catcagctct tttttaaaat tagatgatga
      541 aagtaatgag ttttggacga ttggaaccgt tgttcctatg aatgattttc tgattgagac
      601 aacaaatatt cgcgaccgtg tgacatggat tatttttatc gttgtcggca tttcctgcct
      661 tttaatactt cttctctctc aacgtatctc aaggcccatt ctctctcttg tggaggaaac
      721 cgagcgtatt caaaatcttg atattaaagg agatttaacg ctcaaaagca gtgtccttga
      781 aattcaaatg atgattaatg cgattacgtc gatgcgtaac agtcttctgt cttttagtaa
      841 atttgttccc aaaagtcttg ttaaaaaact tttacaaaag ggcggagaga ttcgccttgg
      901 cggccgcaag aaagacctga cgattttctt ttccgatatt atcggctttt cagcgatttc
      961 ggaaaacata ccccctgaaa aacttgtcag tcacctttca acctattttg atgagctttc
     1021 aaatattctc accacatcaa atggaacgat tgataaatat attggtgatg ggattatggc
     1081 gttttgggga gctccgcttc atgaccgtga tcaaacgttt aatgcctgtc acagtgcgct
     1141 gcaatgtcaa aaaagacttg gtgagcttaa ccgtttatgg gagctggagg ggcagcctat
     1201 ttttcgcaca cggattggga ttcatcgcga tgatgttctt gtggggatta ttgggtctga
     1261 agaaaaaatg aattatacta ttatggggga cggcgttaat ttagcctcgc gcctcgaggg
     1321 tcttaataag tactatcata caggtattac gattagtcaa aatgtttatg atcatgtcga
     1381 gggccatttt cttgttaggc ctttagatgt tattgccgta aagggcaaga caaaggctgt
     1441 gaagattttt gaacttgttg ctcaaaacaa aggcgatcca agtcttttgc ccagcgagga
     1501 tgaccggcat ctatgtgatc ttttcacaaa agcttttcgg ttctataccc tccaaaagtg
     1561 ggaagatgcc ctcgagcatt ttcaacacat ccatacgctc tatcctcaag actacccaac
     1621 acaaatttat atagaaaggt gtctaaattt tcaaaaagag cccccgtcta aggattggga
     1681 cggtgtgact ctttttaaat aaaataaata ttaatagcag cattaaacta atattgatcg
     1741 cttctactga tgcatcttta aaaatacttc gcgcgcaacc tctatcgcca tcgcttggat
     1801 tgttttttca tcactggaca gacgacttgt aaggcttgaa aactcatcat tacggtccac
     1861 attatacgac gaaatcgcat ccgttttccc tttatggatg agttttccat ccttccatat
     1921 ttcataaaaa gctgtaatca cattttggct acgcaaggct gtggcatccc ggccatagct
     1981 tatatcataa atggtttgcg caagcgtcac cttaatcgta aggggatgtg aataggttaa
     2041 aaggttcagt tgttgctgca attcttgata gaatttatag tttaaatacc cctcgatgcc
     2101 attcacactc agtgtcacag gcgttggttt tgacgctctc tcgagtattg gtttaaaccc
     2161 gcaacccgat agcatcgtga cgaggaatac aatcaaaaat tgctgcaaaa tatccacatt
     2221 tttaaattcg tctgctctcg ttagactagc aattttaaaa aataatgtca gctctcataa
     2281 aaatttttaa ccgactacaa tattaataat tttttggggc acaataatca cacggcggat
     2341 atttttaccc tcaatatccc gctgaacggt gggaagtgca tgggcttttt cgcgaattgt
     2401 ctcctcatct gcaccgggag aaactaaaat agctccgcgc atctttccat taacctggac
     2461 ggcgatcgtc atctcatcaa gggccgcaag cttttcatca aaagccggcc agggagcatc
     2521 atgcagatac gttgtttgcc cgagtttttc ccacagctct gaggtcaaat ggggtgaaag
     2581 gggagaaagg gtgattaaaa acacggaaaa ggcataatgg agcacctctt ttgaaacatt
     2641 tttagcgctg aagtcttcta atgttcggca aaactcacgg tgaaaagcaa taattttatt
     2701 aaaaccatag cgctcatagg cctctgttac tgtttttaga aaacggtggg ttgctttttg
     2761 aagggctaaa gagtcctcag gatgaccact attacctgct gtgtgggcaa gttgcgcaag
     2821 aacctgctcg cccacacgcg atactttgct gagataacgc caagcaccat caaggctgtc
     2881 atcattccaa tcaaaatcct tgtcataagg cgtatcggac atcacaaaaa gccgcgccgt
     2941 atccgcacca taggaatcaa taatataacg gggttccacg acattttttt tggacttagc
     3001 cattttttca gagcgcccta cggtcacttt agaaccgtct tttaatagat aagacccatc
     3061 tttttctttg ataacatccg acggctccac ccacttacca tgctgatcct tataggtttg
     3121 atgacacacc atgccttggg taagaagttc agcaaaaggc tcatcaacgt caatgtaacc
     3181 acaatcacgc agcgctttca caaaaaatcg tgcataaagc aagtgcataa cggcatgctc
     3241 tggcccccca atatacacgt ccacaggcat cagacgatcg gccttgtcct tattaatagg
     3301 ctttgtgcag gttgggtcaa taaaacgtaa aaaataccag gatgactcaa aaaatgtgtc
     3361 aaaagtatcc gtctcgcgca cggccttttg atgacactga ggacaacttg tatgcttcca
     3421 tgtggggtga tgttccaaag gattaccgga tttggaaaaa tcaggatcct ctggaagtgt
     3481 gattggcaga tctgattctt tcaccggtac tacaccgcaa tgatcacagt aaacaatggg
     3541 aatcggacag ccccaatagc gttgacgaga gattcccc
//
LOCUS       DAIAHG010000125         3577 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_125_length_3577_cov_3.261714, whole genome shotgun sequence.
ACCESSION   DAIAHG010000125 DAIAHG010000000
VERSION     DAIAHG010000125.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125_length_3577_cov_3.261714"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1073 a          756 c          733 g         1015 t
ORIGIN      
        1 caaatcaatc cgctttgtag gattattatg cagcaaccct tctgtcatta aaaaagcgta
       61 aaattggcgc aaggccgata attttcggga caatgtcgca cgttttgctt ctttttgcgc
      121 ctcaacaaac gtaaggatat caatgtcact cacattcagg ggattctttt gaatatgttg
      181 aaaaaaaagc acaagatccg aacgataggc agcgatcgtt tgaaacgatg cccctctctc
      241 ggacgccatc atttctaaaa aaagatcaag ataaagaggg gaatgcacca acgctacact
      301 ttttttctta aacgaaattt acttgatatt aacgaattag ttttacctta taaaataatt
      361 tttttagcga catccatttg cttggcagga attccgatga caccacataa aacaatccct
      421 acaaaaaaaa ttaaaaatat gctaaaccat agaaatcgca tcataacgcg atgcacccaa
      481 agcaaggttt tctaaacact cagtttgtca caaccttctg ttggatgcaa tggaaccttt
      541 aacgtcagac gctcaagatt aagataggct tcgatcaatg aaacaagatt acccgaataa
      601 taaatcactt gttggaacac tcagctttac ccctccgcgc accattgtcc tcatagggct
      661 tatgggcgca ggcaaaacaa gtattggaaa acgcttggca aagcgccttg atctcccctt
      721 tcacgattcg gatcatgagg ttgaaatagc cgctggttgc ccgattaaag aaattttaga
      781 aacctatggc gaagaagctt tttttagtgg tgaataccgc gtcattggcc gcctccttga
      841 tcaacctacg cacgttcttg caacgggtgg cggatctttt atgacagaaa aaacacgcga
      901 aaaaattaag gaaaaagcca tttctgtttg gctcaaagca gaccttgaaa cccttgtcac
      961 ccgcgtaagc cgaaggtccg atcgaccttt tcttaccgat ggtaatcaac gcgaaacatt
     1021 agaacaactt attgaagagc gttatcctta ttatgaaaca tgcgatgtag ccgtcgatac
     1081 atttgatgag cccacagcag ccaccgtaga gcgcgttatt gttgccctta ccgagtacat
     1141 tcgcgtgcac tatcctcatc agtatattct taaatccgtc gagggataat tttttgggac
     1201 gatacattct ttttattctt tttttgattt atggcccaga gcctttcaag gcttatggac
     1261 aaacgaatct ttcagcctcc agtcgcttgg aggtttgccg gacaaaactc aaaaaaatta
     1321 aagcagaagc tgtgcgcctt ggtaaacttg agctctttga aggacttttt gatcaagcaa
     1381 cgcaccttct tgcacagatc aaagaagaaa ctgagaccac agaaatttat tttcttttag
     1441 gcgaatgcca aggcgttctt gagagcttag aatttagtct taaccttggg aaatatagta
     1501 aaaataatcg acatgtggag atttcttctg aaaaaacaga aaaacccatt ataaagcgtg
     1561 acaaacgaca agggacacct cgcccgatat ctgccatcaa agaacctgtg ccaatgaaaa
     1621 atatagatga agaagatgag gaaacgctaa aggactatac cccgattaaa agcgcatcaa
     1681 gatataaaaa acaacaaacc gtcgaggatg atttggagaa ggatatacat taaaaaatat
     1741 ggccatcgaa cttctcattt tttggacgct tatccctttt cttctcttcg tatccgcgtt
     1801 ttttgctgcg agtgaaacgg ctatcacagc agcgtcgcgc gcaaaaattc accagctcac
     1861 acgtgatggc gacgagcgtg ctaaaattat caaagatctt cagagcgatc tgggcctcag
     1921 cattagtgca cttttgctcg gagctacttt ttttaataca ctttctgtat cctccgctac
     1981 agcccttctc attcgcatcc ttggccaaga aggtgtggtc tatgcctccc tcctcatggg
     2041 cgttttgatt attatttatg cagaagtagc gcccaagatt attgccgttc aaaatcctga
     2101 aaattttctg ttacgctcgg caaaaatact gcgcattctt tttattatcc taagacctat
     2161 tcttaatctc attaatttta tcgcgcggct ctctttgcgt atttttggcg tgaaaatttc
     2221 aaatactcaa acaagtcatg ccacactcga ggagcttcgt ggcgtcattg atctgcacca
     2281 tggtcccggt gatgatacag ccgacgagcg tgcgatgctc aaaagcatcc ttgatcttgg
     2341 cagtgtccaa gtcagtgaga ttatgaccca ccgtaaaaat gtcacgatgt tggatgcggg
     2401 agatcctcct gaaaaaatag tatcatttat cctccaaagc ccctttacac gctttcccat
     2461 atggaaagat gatcctgaaa atgttgtcgg tgtcattaat gcaaaggccc ttttgcgcgc
     2521 tgttaaggct cagcccgagg gactcaaaga ccttgatatc ctaagcgttg cccataaacc
     2581 ttggtttgtc cccgacagca cagatttatt agaacagctc aaggcctttc gcgatcgcca
     2641 tgagcatttt gccattgttg tggatgagta tggcgccctt caaggcattg tcacgctgga
     2701 agatatttta gaggaaatcg tcggcgagat tagtgatgaa catgatatta ccgtcaaagg
     2761 tgtaaggcct cagtccgatg gatcttttat tattgatggc aatgtgacga ttcgcgattt
     2821 aaagcgtcag tttgagtggg atcttcccga tgaagtggcg tcgaccctgg caggcctcat
     2881 tctctatgaa gtgcgccgca ttgccgatgt gggacaggtt tttattttgc acgggtttcg
     2941 ttttgaaatt cttcgaaagc agcgcaatca gatcacactc attcgtgttt ctccccctca
     3001 agttcaagaa aataatgatt aaaactccat aacggtacag ggattttttt aaaatacttt
     3061 tgttgctttt tcttttataa aaagagtgtt gtcactgttt ataaggaaaa aatgatgttc
     3121 acattaacat tatccaacaa agtactgatg ctgtctttgg ctgtttgtgt cgctggcata
     3181 gcacaaagct ttggcatgga cgacgataac aaaattactg aggaaaagga ttctaaactt
     3241 tctaaaatca cacatatttt taaagagtct caagagacgc taaaggatgt tttcattgaa
     3301 gagataacct gtattttgaa taaagcaaag gatttagata tcaagcatcc tgatgagaag
     3361 gttggaatga gcgaactgaa tagaaataaa gtttggatga cactatgggc tatcaaagcg
     3421 cgtctcggta tgggtatgaa gagcacgtcc gagagatatg acgcatttga agagatcgga
     3481 aaacttgtta gccccgagat tcttaatctt ttggaaacct ttggatctga ggagaaggat
     3541 tttataaaaa aagcttttca aaaagctttt caaaaag
//
LOCUS       DAIAHG010000126         3543 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_126_length_3543_cov_3.053664, whole genome shotgun sequence.
ACCESSION   DAIAHG010000126 DAIAHG010000000
VERSION     DAIAHG010000126.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126_length_3543_cov_3.053664"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1233 a          753 c          579 g          978 t
ORIGIN      
        1 aaacattcat gatcttgcgc tatccaacaa aagataaacg catctcaaat taatttcgga
       61 ccctacttca aaaaacactg acaggttatt atttctaata acatatcatt ttgtatgttt
      121 attactgtct attctattcg ctgctccatt cttagcatga acattacccc ctacactccc
      181 cttatatctt ccaatataaa gcgcacaggt taatataacg ttaacaatcc ctaaaaattt
      241 aacagctcca gtatcaaagc agcatcctta aacgctgcct ccataccttc cttttctcct
      301 cttcactttt caaccgatca agctcgtttg ttgtgtcccc tttctaccaa cccaaacaaa
      361 aactgtcaat accttttttt cacaatcata cctttttctt tttcactcct ccagataagg
      421 atggtaaccc cccgcgcgaa gtgctttgta ttgctaaaat tagcgcattt attaacggaa
      481 ttttatcttt ctttttttat catcagaaat gacgagtgcc ctatgccggg aggttcacca
      541 tgcctttgat cctatgcttt ctcatcatta tgatgatcag cccaaatacg agcacagcaa
      601 gcgagaattc ttttgaggag atcattcaca aaaaagaaat aacacgaaaa ctccccacag
      661 gatttgaagc agaacttcaa gaaaaaacac ttaatctcga ggatgagctc aagaaaaagc
      721 gcacacatgt ttttaacgat gtggtgtctg aagtaaaaat aatgatgaaa aaaattagtc
      781 tttcggaaga gaatattccc tttttctcgc attatttttt aaaagcgacg cagaacctga
      841 ttcccaaaag cctaatgatt gatacatttt taggaattca cacccatcaa gatctcgcac
      901 tctctttcct tcggcttggg acaagcgccc tcaatgccga tcttttcaac acaaccaaaa
      961 atgtggacaa aaaaatctta gaggcctttg ccacagataa ttattatttt acatttcagt
     1021 ggttttcgca ccacttatca gatcttttta atgacctaag cctcttgccc cctcaccatc
     1081 attacgaaga aacatcgtct ctttacacaa ttcaagggcg cggcaaaatg cttgaaagca
     1141 tcatcagcct tttccaaatc gggcaatggt cactcttaag gatggataaa gacgatgaac
     1201 gccgtaagaa tattatcctt tgtgccgaaa atatttttaa gaacacaaca tttttttata
     1261 aatatcacgc gcaaagaacg cctgattggc atgacatcat tgaacacagt ggcagccttt
     1321 caaacatggc aaaaaatatc atttttatcg aaagtcccat cggcacgaat gatctcaaaa
     1381 atattttatt taaaacgtat acgcaaacaa tgaaaggagc tttaaaccgc gatgaagaaa
     1441 cattttatac actcaaagat tccaatgaaa aattaacaat gggagatttt cacgatcttt
     1501 taaagctaat gtattttgaa tccatagatt tttatgaaaa tcatcttttg aaaaattata
     1561 aagaacacta caaaaccatt gaagattcat ttttaaaaat agtaaatgat aaaaacgaaa
     1621 aaaataattg attgatttaa aacgaatatt atagaataca caggaaaaat attttgggag
     1681 acacgtatga ttaaaacatt actcaccctt gcaatgatga cgagctctgc tttggccgtt
     1741 aatcttgcca tcaaacttga gcttacagag aacgctaaga aggttatcaa cagcattctt
     1801 gataagggca cacttgatga aaccacaaag aaaaagctcg tcaaaacccc ccatatcacg
     1861 atcggttata ttgaaaatat tgacagcgaa aaaacagcta aaaaaatttt taattcagcc
     1921 aaaaatttcc tcgaaaaaga gttagaagaa aattcttttg attattatgt tgaagaaatg
     1981 gcgattaaat ttgattctca cacggttctt gcgcccacaa aaaacactga aaagaatctt
     2041 aaaaaactca atggaaagct tgaggagtat gtgaataacc taggatataa atttaataag
     2101 ttcacgacaa acgacaatta cacccctcac atcacactta ttaatggaac cgttgggcct
     2161 aatcgcctca aatccatgaa caaagaaatc gataagatta aagaaaagaa aaacggcaac
     2221 cttttcttta aaattgattc catcggatcg ggtattaaaa aataagaaga atacccacaa
     2281 caaaaaaacc caggtcaccc tgggtttttt aataacgtgc cattcaaaac acgaaagttc
     2341 tttagcgcga gtaatactca atcactagat taggctccat ttgcaccgga taaggcacat
     2401 cagaaagctt tggcccacgc gtaaacgttc ctgtaagttt tttatgatca acatccatat
     2461 aatcaggcac atcacgttca tttgactgaa cagccgtcat catcgcagga ttctcaagca
     2521 tctttggaga taccgtaatc acatcaccgt cacgcacaat ggctgaacca atattcatac
     2581 gacggccatt tacttttaca tgaccatggc tgacgagttg acgggcagca aacacagttg
     2641 atgcgaattt catacggtac accactgcat ccaagcgacg ctctaaaagc tcaatcatat
     2701 tttcagatgt atcacccttg cgacgcgcag cttcctgata aattttgcgg aattgtgttt
     2761 cgctaattga accatagtac cccttcagct tttgctttgc acgaagctgt aacccatagt
     2821 cagagatttt actccgctgc tgaccatgct gaccaggccc atagttgcgc gtgttaatag
     2881 gactcttggg gcgaccccac aagttaacgc caaggcggcg atcaatttta tgttttgctg
     2941 taatacgctt tgtcatgaat aaataaaacc ttctattggt ttacattgtt agcactaata
     3001 ttgcccccat tcctacccga tttcgaggct tctgtcaaca cttccgtttc actagaaaga
     3061 atatatggaa aatatctgga aaaatgtttt ttattaacta aaccgtaact tttgcctggg
     3121 atactggaag cagacaaaga gacatctact tagaggacta accccatgaa ataccttctt
     3181 ttaatgacga gtttcctgat tgctatcgcg tccatgacgc cgcaggaggc ctttgcagca
     3241 tcccaaaaaa aagttgtaaa aaagaaacct gttacgaaga aaaaggtcgt aaaaatcgaa
     3301 gaggatgaag aagaggatta cgttcctccg aagaaaactg caacaaagaa aaaaacacct
     3361 gtgaaaaagg tcgtaaagaa aaaagttgag gttgacgagg aagaggaaga tgacgaggaa
     3421 gtcgaattta aagccttaaa agccaagaaa gaaattaaat agcctaagcc tgaacccaaa
     3481 gaggctagtc aagtaactat ttatgagttt taaatgttaa aaagaaacaa ccttaacaaa
     3541 aac
//
LOCUS       DAIAHG010000127         3458 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_127_length_3458_cov_2.478852, whole genome shotgun sequence.
ACCESSION   DAIAHG010000127 DAIAHG010000000
VERSION     DAIAHG010000127.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127_length_3458_cov_2.478852"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1011 a          719 c          651 g         1077 t
ORIGIN      
        1 cggtgagggc gcattttatg gtccaaaact cgaatttgtt ctcacggatg ctattggccg
       61 tgactggcaa tgtggcacgc tacaggtgga ttttgtgttg cctgaacgcc ttgatgctga
      121 gtatgtggga gaagacggca agcgccaccg ccccgtgatg ttgcaccgcg ccatttttgg
      181 ttccttcgag cggtttattg gaatgctgat tgaacattat gctggaaagt ttcctttgtg
      241 gcttgcgcct acacaaatcg tagtcgctcc cattacaacg gaggcagctg tgtatggtga
      301 aaaaatttta gcggagcttc gtgcccatgg tttgcgtgcg gaaattgaca cgcgcaatga
      361 gaaaattaat tacaaaatcc gcgagcactc tcttaaaaaa gtcccgatta ttatggttgt
      421 gggcgcccgg gaggctgagg agggaaccgt ggcactgcgc cgtcttggtg gcgaaacaca
      481 gcaagtaatg tcttttaagg acgccctttt cacaattttg cgcgaggcag aggcgcctta
      541 aactaaggtg cctcaaactt tcccgttgct gttttaatga ttcttatccc actcctctaa
      601 gcgaccaaaa aattcccagc caggagtgaa aagttcatca tccccatagg gaacatactt
      661 tcgaccattg gtgtattgaa aataagccat gcttatagaa gtattagagc catcatacca
      721 tttgaggagt gagttaaagt aaagagggta aagtttttcc tttttttctg tccgtacttt
      781 ttcctgccac ttaatcgaat cctcttgcat ttctttatat tctttgacaa gccaattaaa
      841 ataaccattg aatttttcag tataatacag cggatattcc tccctatggc ttgcataggt
      901 aggttctacc cccccggtag cataacagag gcggtgaagg caattatttt attcaattga
      961 accataaaaa acacctcaaa acatttttga tcaccccccc attgtttccc gtaagggtta
     1021 acaaaaagtt aatatttcta tctaataagc accacaattt tgcgcgaggc cgcagcgcct
     1081 tagataggaa caagcttaat cttcggagaa atcattctca gactcataaa ctgtgggtat
     1141 aattgagttt aggaggtccc tctccttttt gagctttttt ataatttctt cgaattcgct
     1201 gtagaggtct acggactcac ctatacaata ctcagaaaca ggtccattgc cagtatcaat
     1261 ataacgcagg ttactctcag cgtccatgtg aacgtcctct aatagtttaa tcgtattagt
     1321 tttttccctt atcattttcc taaactgctt aggataggct gagtagagct tcttcatatt
     1381 ctccaggtag tccttttgaa attttgaagt gctataaaac tttttcgttg tgactttcaa
     1441 aggattgaga gaatcaaaaa attttttaga aaacgcaacg ctttccccca tcatcaggag
     1501 tgagatcaaa aaaataaaga ttttcataaa aatatcccta aaaaatagtc agcacaatga
     1561 tgataagcga ggcagcggca tcttagtata ggtcgtcatc atactcaatg aaaggtttcc
     1621 atggctctag atctttctct aattcttctt gtatatattc tatctccttt ttgacctcat
     1681 tagagcttag attttcaata tttgatgata tccactctaa attttctaat gtctttcttt
     1741 tgcgatctac tatacttttt atgttgccct tgtatttttt atcgtcataa agttctctca
     1801 gttttttgta aaaagcagta ccaaaatctt ctttcgtcga gtaataacga aggttttgca
     1861 cactcatctt tttcagagtt ttttttaaat tcaaaggatt gagagaatca aaaaattttt
     1921 tagaaaacgc aacgctttcc cccatcatca gaagtgagat caaaaaaata aagattttca
     1981 taaaaataat ttaaaaatgt tttatattta ccctttaaca tactccttca aactcagcaa
     2041 caggtccttt tacagagttt tttcattttt tttgacaaaa gtcccagttt ttttagtttt
     2101 ttgccttttt ccacacattt caggattgac cgatgcattt tatgctgatt acattatctt
     2161 tgtaggtaca ttctaacaaa ggttaattcc cgtgaacaaa caagaactcg ttgctcaaat
     2221 ctccagccac tctggtctta caaaagcaga tgcagaacgc gctttggatg caactattac
     2281 atctgttacc catgctctaa agggtggtga tgaagtccgt ctcgtcggct ttggtacatt
     2341 tacaacagca caacgtgcag cttcgatggg acgcaatccc cgtacaggcc aagaaattaa
     2401 aattgcggcc acgcgtcttc ctaagtttcg cgcaggcaag caacttaaag acgctgttgc
     2461 tccttaggtt ttaaaaagtc agcagctgcg taagataaaa atgtattatt ttcttgaaaa
     2521 atattttgaa aatggttaga agtagtctta cgcagctgac atcatcggtg ttagctcaac
     2581 tcaaaagggt gattagctca gcggtagagc atctcgttta caccgaggcg gtcggcggtt
     2641 caatcccgtc atcacccacc atttttttga ttctccttct tctcctttca tcttgtggtc
     2701 gcaagggatc tcccgagccc tttgagggcg ttgactaccc tacgacttat cccaaacgct
     2761 aaatttcctg cgtaatcttc tcttgacttt tacccccttt gtgcaaagat ttttaaagcg
     2821 atctcccata aaatagggtc tatgcgaatg ataaaaatag tctttagtct tctaactctc
     2881 tttgccttcc ttatatcgag ccatggttct tcgagcacag aagaacctct ttgcccatgg
     2941 agcgagctct atgagacgaa taatacgctt ggaatacttc aacagcgtct tcagaaaaaa
     3001 aatgaattaa acgaagaaga ttttctcaca ctgaaaaatg atattaacaa tctttcaatc
     3061 cctgaaaagt taacaaatgc caaggtttca gataacacct tttgggagca atataatcaa
     3121 acgctctctc atctgagtag cctaaaagaa accatcaaag aagttgatga aaaaaagaat
     3181 attagtaatg tgccagattt taacctcgat gaactgaaaa agcgcctcaa tgagcttgaa
     3241 aaaaagaatg aaaatctcga ggttcataaa attccttact ttaaaatcac tgatgaaaaa
     3301 aattatgagg aaacccaggg ggaaataatc aataatcttt acaatgaaaa taatgaggaa
     3361 aattagatta gcaggctgcc gcttaaatgc tcctgcggtt ttctggtggt gtcaaaatgc
     3421 tttatggggg gatgcagatg ggcatttttc tcccattt
//
LOCUS       DAIAHG010000128         3451 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_128_length_3451_cov_3.695021, whole genome shotgun sequence.
ACCESSION   DAIAHG010000128 DAIAHG010000000
VERSION     DAIAHG010000128.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128_length_3451_cov_3.695021"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          984 a          842 c          635 g          990 t
ORIGIN      
        1 acagtttttc cttcaagcca ccaatcggca gaacccgccc gcgcagagta atttcgccgg
       61 tcatggcgat atcccggcgg ataggtatgc cggtaatggc ggagacgatc gaagtggcca
      121 tggcgatgcc cgcagaggga ccatccttgg gtgtagcccc ttcaggcacg tgaatatgaa
      181 tatctttttt gtcaaaaaga cttggatcga tcccaagttt ctcagccttg gagcgcacat
      241 aactaatcgc cgcttgggca gattcttgca tcacttcacc aagtttcccc gtgatttgcg
      301 ttttaccttt gcccggcaac ataactgcct caatggagag aagatccccc cctacttctg
      361 tccaggcaag ccccgttgtc acaccaactt gatgctcaag ctccgcctca ccgtattgaa
      421 agcgcctaac cccagcatat ttatgaagat tttttgaagt aattttaata cttttcgcct
      481 tttgcatcaa aatatctttg atacttttac ggcaaagatt cgccacctcg cgctcgagat
      541 tacggacacc cgcctcgcgt gtataatagc gaatgagatc tttgagcgca tctgttgtta
      601 cagaaaactc acccttttta agactatgca atttaatttc ttttgggacc agatgacgca
      661 aacaaatttg aattttttcg tcctctgtat accctgataa acgaatgact tccatgcgat
      721 caagaagggc ttgaggaatt ttaagactat tggccgttgt cacaaacatc acatccgaaa
      781 ggtcgtaatc aacctctaaa tagtgatcat taaaatgact gttttgctca ggatccaaga
      841 cttctaaaag cgcagacgaa ggatcacccc gccaatcagc gccaagttta tcgatctcat
      901 ccaaaagaaa aagaggatta cacgtttttg cttttttcat cccctgaata atttttccag
      961 gcatcgagcc gatataggta cgcctatgac cacgaatttc cgcttcatcc cgcacaccgc
     1021 caagagcaat acgcacaaaa gtacgccctg ttgcctctgc aatggacttg ccaagggagg
     1081 tttttcccac accaggaggg ccaacgaggc ataaaatttg tccacgcacc ttacccacac
     1141 gggcctgtac cgcaagatat tccataatgc ggtctttaac tttctcaaga ccatagtgat
     1201 cgtggctaag aattttctca gcctctttaa gatcatggcg aatacgcgtg cgtttgtgcc
     1261 atggtgtgct tagcatccaa tcaagataat tacgcacaac agtggcttct gcggccatag
     1321 gactcatcgt gcgaagtttt ttaacctctg ccatcgcctt atcatgggct tcttttggca
     1381 ttttcgcttt ttttatttta tcttcaaaat cagagacttc atcttttgcg tcttcgcctt
     1441 cgccaagctc tttttgaatc gctttaagtt gctcattcag ataataatcc ttttgagatt
     1501 tctccatctg gcgtttaacg cgcgttttaa tacgcttttc aacgttcaaa acactcattt
     1561 ctgactcaat aaagtttaaa ataacctcta ggcgcttttg cacatcccct atttcaagaa
     1621 gatcttgctt ttcacgaatt ttaagcgaca ggtaagaggc gattgtatct gcaagcttgc
     1681 tcgattgatc aatttgagag attgtcacaa ggacatctgg tggaattttt cgattcaatt
     1741 tgaggtattg ctcaaaatta tcgatcacag cccgcatcag cccttcgact tcgtctgcaa
     1801 cctcattttc agaaggaata atatccccct gcccttcaaa atagcttttc tcagaaaaaa
     1861 actgcgtcac atgcatccga tcaagcccct caacaaggac tttcacggta ccatcaggaa
     1921 gctttaatag ctgcaaaacc gtggcaagtg tcccataagt aaaaagatct gacgtgttag
     1981 gatcatccag atttccatcg atctgtgtga cgaggaggac cttcttttga tccgattcca
     2041 tcacatcctc aagggctttg atagatttat cacgccctac aaaaagaggc accaccatgt
     2101 ggggaaatac aactatatcc cgaaggggta aaactgggta tttttcacct ttttttattc
     2161 caaacatcga tcaaacgctc tttttcttta tataaatagg tatactacat tagtatatac
     2221 ggtaaaaagt atctttttta ttaattttct cttggatttt aacaaaaaaa cggatttacg
     2281 ctaaaatagg gctagtcttc gcctttttta gccgaagatt tcggtgattt tgccttttga
     2341 tcttcctttt ccaaatgctt aagcagagtt tttttctgct tttcttccca atcttctcgt
     2401 aaaaatggca aaatatcact caacacaggc agcataccga tgcgaagccc ctcagaaaat
     2461 gcagcaatac cacaccccat caatacccaa ataggccaaa aagcccctcc ccccattgcc
     2521 atccacgcaa taacgcacgc gaaaaaaata gcaatgtaca cggtcaaatt aatgtaaaaa
     2581 cctttaattt gtttaacctt ttgacttatt ttttcattca tatcaggtgt tgtcatattt
     2641 ttactcaaac aaaatagtac cttaggaaca gtatacagcg aaaaaagcga ggggatctag
     2701 tctcaaaaat aaaaatttta tagaatctag cctactgtaa ccgtcacttg accaggaaaa
     2761 gtcacttgga tcgtcccccc aagagcacat acaagtgttg atccactgtc aagtgcaggc
     2821 atattgcgca caaggaccgt tggacatcca ggcacccaag gaccaggagt cgccggcacg
     2881 caaggtgaag gggcccctcc ctttgcaata actgtaggat ttgtcggcga tgtgcacatc
     2941 ccaaaattag aaatattcac aggaaacttg ctatccataa tatttgctac ggcacctgag
     3001 ggcccctgaa ccgttggatc gattacctga agaggacaag gggttttccc catactgcat
     3061 tgaagaagtg cattggctgt aacaagcatt cccataaata ttctcttttt ttttagagta
     3121 ttttaattat aattccccac atccatttta gtgaataatg gtaaaaaacc tattccatcg
     3181 aatgccagga atccattctg tcacatccag atcataaaga tcataccaat gactgtcggg
     3241 atgcttgaaa agtcttgctt ccgtcgagaa aaagagaatt tcagcacggc caataagtga
     3301 ttctctcggc acaaaaccta gaacatttgg aagacgacta tctccagaac tgtcacggtt
     3361 atctcccatc ataaagaaat tcccctctgg aacattatac tcaggcgtat tatcaaggtg
     3421 ccccttacca aattcgtctt ttttcaaaat a
//
LOCUS       DAIAHG010000129         3311 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_129_length_3311_cov_3.275510, whole genome shotgun sequence.
ACCESSION   DAIAHG010000129 DAIAHG010000000
VERSION     DAIAHG010000129.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129_length_3311_cov_3.275510"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1017 a          705 c          698 g          891 t
ORIGIN      
        1 tttctaagca tttgtgatcg actgcgagtc caaaaaaaat aagattgtga tattttattt
       61 tttctaaaca caaaataatc cccattgatc tctgcaaagg gaatgcactt tttgggaaca
      121 ccataagtat taaaagcttc gttgataaaa tttcttaggt cccacggaca agcgacgtca
      181 aaagcccctg agaacttatc atatttatac agtgtcaaaa gaattgtttt agcttctttc
      241 gggcactcta tgccacaaag atctttgata aaatcgatat ccccttcgtt agcaccggga
      301 aggccgtaag gataagagtc atttttgatc aagggtaatt tttccgtaac ctctttgatc
      361 aaattatcaa aattttttgt cgccatggca ttccctgtga ataaacaaaa agaaagacag
      421 agtagttgaa gaaagcgtga cttgctcata aaactatttt ttaataaatt ttaacaaaaa
      481 atactatata ttataaatta atcaagataa attttcataa aaaatttaat taaagcgctt
      541 caatgctatt ttctatggca ctagatgcaa aaaagcctca cccctgaaga atcacagaac
      601 cattaaccgg tatgtttttt cgcgtattca accaagtgtt gcacccaagc tgttgcgttg
      661 cgcaaagaat cgtcgcagga acgtcctctt ttttcaaaag aagttccatt gagaaagtct
      721 tgggcgtgct aaaataagaa gccacaatac gattgaggcg atcataggcg gacacagtct
      781 tttgctcttt ttctttggga gacgcgggca gaaaatcgag gaactgctga taacaaagag
      841 ggccaatgat caaacgaaaa ctttttgcct gatcaaagga ttttttaccc aaaatagctg
      901 actcgcctag actatgaaat tttttcccaa gtcgcgtttg ttccgcggga ctcaccgtat
      961 tccacccccc tttgaaagga gcaatgcggc aacgaatatt gaaatagctt tcgattattt
     1021 tttgcagccc tgtcatggat cctgagcgct gccagaaaag cgttgcgaaa ggcgcaaggc
     1081 cttcgccaca atcacttttg atctgatggc gcacaccacc aagatccata agcgattcac
     1141 ccacttctgt attctcaaga acttggtccg tgagccctgg gtagagtttt gtatagaatt
     1201 taaaccaaaa agtgattagt cggtgattaa aaatatcgag aaagtcttta aacgcattct
     1261 ctttaagctt tatccgatca atcaaaattt ctgtaaaaac ctcaggcaag gggccttgaa
     1321 tccctgcaat acctaaaaaa ctgacggtaa gctttggtgg gctgcccgga tgtagttgct
     1381 taagatcccc cgtattcacc cccaagccaa tgtgcgcctc aaagtgcact ggatcaaaac
     1441 gcacatcctc attgtcgccc aatttttgcg aaggctgccc gcgctgaagg agccttacag
     1501 cctgtggaaa atcatagaga tacgtatgtg catacaaatg ctgcataagc ggtccttgag
     1561 attcatccac atgggattga ttcaaacgtt ttttctttga ggatactaaa gaagaagatg
     1621 ttgtccgttg agggggggcc attgcatcca tactcctttc cgttgtcggc tataaagggt
     1681 catttcaata aatgaatcaa gattaatatt catggctaaa aaatgctgaa gaacatgagc
     1741 caagacaaaa ctcataccac ttggatcaat ttcttcatca agggtaagtg acacggaaac
     1801 cccttccaca aaccccatcc acgcctgatc attcacgcac cttttaatac gccgcgcact
     1861 cgcttctact ttaaattcca caagagcctc gatttgctga ccgatggacg cccctgtcat
     1921 tgatgcgtat aagtgcagtg tttctttaat caccttgaga cttgtcgagg ggtctgtata
     1981 gcctaaatga tgaatagaaa gctgagagac aagcttccat aacgtttcgc catccatagg
     2041 cgaataagca ggtgtaacag gtttatcaag acatatcatc cgtgtcaccg gaagttttcc
     2101 atcataataa agtgtcgctc cagcgggaag ttgctctgca agaaagcgat tggtacaaag
     2161 cgttgtcgca aaaatagttt cacttggtgg catcatcgga ttgaaatcat gacttacaaa
     2221 agaaagatac acatctgtgc ctggaatctg tctgtgatca gacgcttgac gacgatggta
     2281 ccaaaaaaga gaactttcct taccatgttc aaaagagtaa taggggcggt attctaaaac
     2341 tgtgccatca tccaaaacgg aatgaacatc cacaatcgaa tgaatctcat gggtacgctc
     2401 caagcgacta tcggctgtca aacgatactc cacctgccgc tgatttacac gcaaagggtc
     2461 tgtggttttt tgaaaaagat taataatcgg tgtgcatcca agaagaaaat tctctggccc
     2521 tatggcaaga tgcgaaataa tttttccatc actcacagga ataaaaatct caagcatatc
     2581 cgtttgattt tcttttgtcg cttttaatag gtggcgcagc tgaaaaaaga aatatttttc
     2641 aggaaaatga aaatactcct ggagcagttg ataggcgggg tgactgtggg caggtgacgg
     2701 gaaaagctgt tcgtcacgtt caaaacctat gggctttaaa gaatcactgg gaagggtttt
     2761 gagattttga ccatccgttg aatagaggat aaaaggatca ctttgaccaa aaatagcttt
     2821 gtaaagactt acagccgtaa gacgatcgcc ccccaaatga aacacaagcg tatcaagatc
     2881 aaggtcacta aaataaagtt tttgggcctt gagagttaat ttaatatacc acgggcttcg
     2941 cggtacagcg tcattaaacg tataagaaag ggcttggaca aaactagcct ccttcacatc
     3001 aaggggccag agcgtcaggg ggtacgccgt ttggaaacga caaataagcc cttcctctgc
     3061 ataggcaaaa agaggtgtat ggcgctcaat agcatagcct gccgtcattt ttgccttggc
     3121 tggatccact tgaaaatgcg ccatggccac cgatggcaac ggattaatca gctgggggta
     3181 gagggtgttc aaaagccctg cggcgatctg tggaaaacga tcatcaatgg tttggtgcaa
     3241 ttgtgcggaa agataggcaa aggattctaa aagacgctcc acatggggat cttttgactc
     3301 tccctttgtc c
//
LOCUS       DAIAHG010000130         3293 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_130_length_3293_cov_2.702114, whole genome shotgun sequence.
ACCESSION   DAIAHG010000130 DAIAHG010000000
VERSION     DAIAHG010000130.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130_length_3293_cov_2.702114"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          911 a          688 c          684 g         1010 t
ORIGIN      
        1 aacaacgctg ccttcgaagc ttttgcgtcc ggccagatcc atctcgagaa gatcggcaaa
       61 actttctttg tgggatgagt gtgatttatc catgataaaa tcctaagtgg ggttagtggt
      121 ttaatttttg ctgtttttta agttctacca ccgaaggaaa aagccttttt ctgggacaat
      181 aaaattcaac tcaggaacga ggaaaaataa aaataaaact taaaaacaac gctcagtgac
      241 gcattgtgac gcattatggc atgcttcgtc aatagaaaga tgggttgtat cgataataaa
      301 agcatcgact gcgacagtta agggatctat ttttcgcgtt gcatcgcggc ggtcgcgctc
      361 attcatatta cgcgtgatat gcgaactcac ctccagcgtt tcttttttcc gacgctctag
      421 gcgcacatgc tcatcagctg tgatataaaa tttagcacaa gcattaggaa aaacggccgt
      481 tgtaatatcg cgcccatcga tgacatagcc gactactcct tctggaggat tatccacctg
      541 tgcataaagc cactgattca cagcctttcg aatccaatcc agcgctgcga gttttgaagc
      601 aacattcgcc gttttttcca aacgcagccg ggcgtcagcg tccagtgttt tcggatcaat
      661 cgttgtgata aagtctttaa ttacttcttc atccttagct gacaaatcat caggatcaag
      721 gtgatgatcc attacataaa gccccacctg gcggtaaatg agaccactat caatatgcgc
      781 aagattaaat tgtgctgcta aataccccgc aattgttcct tttccagacc ccgaaggccc
      841 atcaatcgca atcactagag gcgttttaga cggaagcgtt tggcgtattt gaggctgttg
      901 cataggacga acctttgaaa aattaataat tttctttttg agagtataaa atacatcccc
      961 cctcagagaa aagaaatttt agaacatttc acaggatact attgatttct gtcccctcct
     1021 gtgattaagc tgtggcaaaa gtatgtccta agaatgagtc aattttatgg ttaaagccga
     1081 atgggggatt aaacgcacgt gtcaggcatg cagtgcgcat ttttatgacc tccaaaaatc
     1141 accgattaca tgcccaaaat gccatgcgcc ttacgagctt atcgcaccaa cacgtcgtgg
     1201 acgggggcgc caagctctgg cccttgagga gggcaaactg gccgccattg acgacctcgt
     1261 cttgggtgat gccttggatc ttcccgaaga tcttgacgat gatgccgctg atgcactcat
     1321 cgaggatgcg gatgatctgg atgaaggcct tgatgatatt gatggccttg atcacaaccg
     1381 tgatgatgat cactaaaatc atttaaaaag gggatggcgc ataccacccc cccttttttt
     1441 tagtcatctg ccttgcacag tttttttaat tcttcttgaa ggcctgagac tctttctctg
     1501 ccctttgtgc tgtgcttgtc attagtatca gtcagcatct tcatttggag cctgtcatag
     1561 atcctgttaa ggtcatcttt cgttagctct tccttttcga gagctttctt catgtcagcc
     1621 aacagcaccc gttctttttc tttgaatact ttttcttcgt cagaatgatt tactacatcc
     1681 cacatattat gcatgagttt acctattaat ttatcctttg ggggctttaa gctttctcta
     1741 agcttttcaa tatcagcctc ccaggcaaaa atagtgcccc ttaaattttt aatttcattt
     1801 tcataatact cgatggcatt cttggcagtg gaaattttct ccttaatccc ctcctcatca
     1861 ctcgccctca caggagaaag attaagtgct acccccccat atatattaaa gacataagtg
     1921 tcaagatctt tttcataaaa tattctcttt gttaatgatt attacaccat ctttatacat
     1981 gacagagaaa agtaaaggaa gggttaatct tgcgccatat tttaggaaaa aaagtgcatg
     2041 atcaccccct cacaatttga ttttctagtg cattgcgcct cgctctcgcg ctatagtgac
     2101 ctcagattta ctctaaaggt ttttgagtca tgcaattttt tcttgattct gcagatgttg
     2161 atgttattaa aaaattggcc ctgactgggc ttgtggatgg cgtgacgacg aatccgtcgc
     2221 ttgttctgca aagcggttgg tcctttgagg aggctttgcg cgaaatttgt gcagcggtgg
     2281 aaggccctgt gagcgcagaa gttgtcgccg cggattttga gggcattgtg aaagaggggc
     2341 tccatctcgc taaaattgct aataatattg tggtcaaggt acctttgacg tttgatggac
     2401 ttaaggcgtg tcaggtcttt tgcgttgagg aaattcctgt gaatgtcacg ctttgttttt
     2461 cagcgtccca ggcccttatg gccgcaaaag ccggtgcgac ctttatctct ccttttattg
     2521 gaagattgga tgatgcgggg cacgatggtc ttcaccttat ccatgagatt gcgcaaatct
     2581 atcgccagtt tgattttgag acacagattt tagccgcctc tattcgccat cctctccatg
     2641 tcattgaggc ggcaaagctt gggtgtgacg ccgcaacgat tccccccaaa atttttgaac
     2701 agctgataaa gcatccgctt acggacaaag gtcttgagaa ttttacaaaa gattgggcaa
     2761 aaacagggca gaagatactt taagcgtaga gtgggagaaa gaagattttt caaggtttcg
     2821 aattgatttt tgaagtttct ggggattttt ttctcttaaa atctcgatgg aaagggagtt
     2881 tttcataaaa aataacggag atattacaca attattcttt aaaaaggcac aaaagaatga
     2941 aaaaatcttg taaaaaatga ctattttgag gaaaattaga aaaatattgc taaaaaacta
     3001 aaaaagagag ccaaccgaac cataataatg atgcaaataa aattttcacc ttaccttgag
     3061 catagagcaa aaacattaaa aaagagttaa ttttgaaaaa acctctaaca cgcataacga
     3121 ttgcttaaaa atgcttctca atatgcgttt tttcaatttt atgggattat tttagtgatt
     3181 tttcattgaa tattcgatga aaagggtgct tcttcagtat tttagtggca tttcgcagga
     3241 attttcctgg atataacgcc attttttggt cttatgaggg cttttccgac gag
//
LOCUS       DAIAHG010000131         3247 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_131_length_3247_cov_2.907571, whole genome shotgun sequence.
ACCESSION   DAIAHG010000131 DAIAHG010000000
VERSION     DAIAHG010000131.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131_length_3247_cov_2.907571"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          947 a          721 c          663 g          916 t
ORIGIN      
        1 aaaggtccca ccgagtgttc ccatacccat tgtgatagaa gtcaaaagcg caaatttgcg
       61 aggagagaac cactcagccg cgatttttgc cgtacttaag taagcacatg ctgatccagc
      121 tcccattaag aaacggccga attctgcata ggcaagatta tcgctccttg cgaaaagaac
      181 acaacctact gtgcaaaaaa gagaggaaaa cgtgacaaca cgccgcgcac cgagcttatc
      241 aacaatcaca ccacagggaa tctgcagcgg cacatatgca taataataaa aagaaacaag
      301 cacgcccaaa gacgcggatg taacgccaaa atcctgcata agctcattgg tcatcacact
      361 gggcgaaacc cgtaatatat attcataaag gtaaaaaatc gccgcagata tccaaataat
      421 ccacgccttc caatgattgt caggagttag caattttttc tcagaagagt cactatgatg
      481 aagggcccca tccattgtgt attttcgcgt actttcaggc atgcaactaa gctttttgta
      541 atataattat aatgtctagc ctaagcctat tatctaatta cacttttgtc caacagataa
      601 tgcacgattt ttgtatattt ttatcgaaga tgttgcacta aggacaacaa tcgcttggat
      661 ctattgcaca agaaaaaagt ttttcctcat aagatatcct tgcatttacg ccattatttt
      721 tatatagtgt gtctaatttt tatatctaaa cggagaacaa aatgagtgcg caagaaacgc
      781 ttgcaatgaa aaaacgtgat gcttttggca caggagctgc ccgtgaatgc cgccgtagtg
      841 gtatggttcc agggattatt tatggtgcgg ataaagcgcc cgagggaatt gctgtggatc
      901 caaaaacact gtcgaaagaa cttcataaat ctgggttttt ttcccgtgtt ttccacctcg
      961 atattgatgg tcataagcaa caagcccttg tgaaagatat tcaattgcac cccgtcaccg
     1021 acatgccgtt gcacgtcgac tttatgcgcg tggtcaaagg aacaaaaatt cgcgtgagca
     1081 ttcctattca ttttattaat gaggacaaag cgcctttgct caagcgtggc ggcgttttga
     1141 acatcgttcg ccatagccta gagattacag cgcctgcgga tgctatgcca gaatccttta
     1201 ccgtcgatct ttcagcgctt gatctctcca aaggtattca cctgagtgat ttaacaatga
     1261 cttcaggtgt tgcagctgct tacccagcgc gcgataatac ccttgcaaca gtggttatgc
     1321 caaaagaaga gaaaaaagaa gaagctgaat agttttttca tgcgaggata cggggccgct
     1381 cgtggctctc gtgttctcgt tttttatcta ttgtcactcg actctttttt tggattttct
     1441 atgatcattt ttcttaatgg ttgctcaagt gctggaaaat ccacaattgc aaaagccctc
     1501 caatttctct ctgacaaacc gctcttatgc ctcggtatcg acacatttct tgatatgatg
     1561 ccccgtcatt atattggcat gggcaaaaaa gcggccgagg gcattcattt tattccggat
     1621 caaactgaac aaggcgagcc gcttattcat attgagtgtg gtgcgtatgg caaaaaattt
     1681 atgaatgcgg tggcccccgt tgttaaagca cttgctgatc aaaatcttga tcttattctg
     1741 gacgaggtgc tgattgatga ggcgcatctg attaattatg cagacgcgtt aaaagatcat
     1801 accgtgtatt ttattggcgt gcactgcgca cttgagatct tagaggaacg cgagatgctg
     1861 cgcggtgacc gctccctagg ccttgcgcgc gcccaaatta attgtgttca cgcacttaca
     1921 tttttttatg attttgaggt tgacacaaca cacacctcgt ccatgacatg tgcaaaaaat
     1981 attttggcct ttattcaggg caacccaaat cctacgtctt ttgaaacatt gcgggaaaaa
     2041 agttgagcca ttttttctcg ctaggatttt agtgcaagca taacttttct ggtctaagct
     2101 acagttaaac cttccaaagc ccctccataa tcgtcacagc tttaccctta agatgcacac
     2161 gatccttttc aatagaaaga tgaatttcgc cccctcttgc acttaattga taagctttaa
     2221 atgttgtttt cttaagcttt ttttgccaat aatcggcgag tttacaatgc gccgaacctg
     2281 tcacaggatc ctcgtctacg cccacgcgcg gagcaaaaaa gcgcgataca aaatcataat
     2341 gctgactttt tgctgttacg ataataccac gtgcatcgat ggtattcagt gccgcaaaat
     2401 ccggtgtaag atttttcaga acctcttcgc tctctaacaa aacaatcaca tcatcatggg
     2461 cttgaacagc ctcgatcaca tgctcttctg caccaagtgc ttgcgccaaa aaagtatgat
     2521 ctatcacagg gcccgttggt tggagtggga aatcgagaat aaaaaaatca tcctcccgcg
     2581 tgacaaaaag agggccagaa agcgattcca gctgtatttt attgtgcgtg aaaagccctt
     2641 gctcatagag cgtatgacac gccgccagtg ttgcatgacc gcataaacgc acctccacag
     2701 cgggtgtcat ccagcgaata tgaaaatgat ttcccccaag aggtttgaca aaagctgttt
     2761 ccgaaagatt gagttcagcc gcgatgtttt gagcatccgc gggaaaatca tcaacaatca
     2821 tcactcccgc tggatttccg ccataagctt tatgtgtaaa tgcatcaaca atccaaattt
     2881 ttcgtgccat tttcacttcc tctttgtatt cattctgttt tatattgagc atggataata
     2941 taacaaagta aagaaaattt tatgaaaaaa attctatcaa tctcttttct tgcactgtct
     3001 ttgtgcacaa acatttatgc ttcgaatcat tatatccctt cttttgaaga cgtggagggc
     3061 gcaaagaaaa aacttattga ggccaaggat agatacccta ctttgaatga tattgatatc
     3121 tttatcgtgg ggatagaaaa agggactcca gaagcactcg atctctttat tgaaagaaag
     3181 aaaaaagact taggaggtat agagctatcc gaaaaggagt ggctgattcc gtttccaaag
     3241 tggagag
//
LOCUS       DAIAHG010000132         3210 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_132_length_3210_cov_2.868816, whole genome shotgun sequence.
ACCESSION   DAIAHG010000132 DAIAHG010000000
VERSION     DAIAHG010000132.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132_length_3210_cov_2.868816"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1125 a          638 c          674 g          773 t
ORIGIN      
        1 attgagtcct ggcccatgga tttatttgaa cccttacctc tcaatgttat ttcaaagtgg
       61 gaggactttg ccgatggtgt ttttcgctta gagggacgcg tcgtcacaat tctcaataaa
      121 gaaaaaatat tgaaaaaatt attttaactt aataaaggag tacacatacg atgataaaaa
      181 ttattttact gacggtcttt atttttagca taaatgatct aaaagccatg aaagttgatt
      241 ataaaagcat cgactacacg aatacttacc ctgttcctga aatctccttt gagcaataca
      301 caaattttta cacagtgaag aatcctgttt tcacaacgaa tgatatttta aaaaatttat
      361 ttgtgggcga tgacaaagag cctgattttg agaatggtaa aaaagcaata aagcaggata
      421 tcgataaagg ctatgcccac ggttatcatt attatgcgaa tgccattctc aaggaagaaa
      481 tgcttaaaaa aagtctattg aataagagct ctaaggaaac aaaagaatcg attcaatatg
      541 tccatgaggg gtatttacaa tcactttatt gtggcgcttt gaacgcagat aatgactgca
      601 tcaaggatct tatcgctgtt tttgagggag tgggtgaatc tgagctcaaa aaatcatatg
      661 aaaatctttt aaagactttg gagggctata aaaaaaaatc aaacaaaacc catgtttata
      721 gatatcccga cgaagacgaa aaggagggta attgcctcat cagttagctt tgattacttt
      781 ttattctttc aaactccttg ggtagtggcg cttcaaagtc gtaattttga tcaaagaggc
      841 taaagcgtat tttccacgca tggagcatca agcgatgaaa aggctccccg ccatactttt
      901 tatctcctcg aattccgtgt ccaagctcac tcatatgcac acgaagctgg tgagttcggc
      961 ctgtttgagg cgatagctcc accaaggtta cattttcctg ttccacggcc tctataactt
     1021 tataaaaagt aacagcttct ttgccgtgtt cacttagaga gtccaccttc tcaaagcttt
     1081 tttgagactt agagagtctg cttttaatga ccccctctgt tgattcaaaa atcccctcac
     1141 aaagtgccac atacgttttt aaaacagtac gatggcgaaa agcatttgta agctctgtcg
     1201 ctgcttgaat atttttagca atcactaaaa ggccactggt ttctttgtca atgcggtgcg
     1261 tcagacgcaa acggtgctga ggcgaatagt ttcttaaaag atcatcaatc gaggtgcgct
     1321 ggcctgttcc accctgtgtt gcaaggtgtt ccggtttatt caagacaagg aggtggtcat
     1381 tctcaaagac aatcatcggc tttaaaatgg cttttgcctc atcaatggaa agatcaggag
     1441 gtgtaacggt ttctggcggc ggcatggaca ccgcatcatc aggaaatgtt atgagattgc
     1501 ccacaacaag tcgtgtggat gcttttgctt tttgcgcatc aatgcataat tttccttgac
     1561 ggctccactt ttcaaaaagg ttttgaggaa aataaaaccc cctacgacgg aggtaccgat
     1621 caaggcgaac gccctcacca tcttgtaaaa ctgctagttg aggcatataa aaaactttct
     1681 ttgagcaagc ccagatcata aagggttcaa ggctatttgt aaagaatgct gcaaaggcaa
     1741 caaggagctt taacttaaaa ttaaggattg aatgctctaa tagcggtaga ccaaagtcac
     1801 ttaattattc tttagcatga tgcgcaagtt cctcgatctc ctcaaactct gtcttctggc
     1861 tttcggtatg agccttggag tcctttgcat tgtgtcatcc tccacccctt atgtttatgc
     1921 tgctgacgac gatgacgagg aagatactgg agatgacgag gaagacactg gagacgatga
     1981 ggaagacact ggagacgatg aggaagacac tggagatgac gaggaagaca ctggagacga
     2041 cgaggaagag agctccacac aacaaaaaag atctgtgatt aaagatgtga aagatgttgt
     2101 caaaattaca aaagatgttg ggaaaatcgc tagctctatt tttggggctt ttggtggaaa
     2161 aactaaaaag aataatgatg atgacgagga tgacgaagac tatgtccctc ggaaaagaac
     2221 gcagcagcaa caagtagacg atgaagagga ggaggaggaa gaagaagaaa ataatgatga
     2281 cgaggaggaa aataatgatg aggaggaaga agaggaaaca cccccacctc aaaaccctcg
     2341 caaagaagcg cctaaaaaaa aggtagatcc taaggttctt caaaagctcc tatcagatat
     2401 cactaagcta gaaaataaaa ttcctgccct tgagaaaaaa cccgcagacc ttaaagttct
     2461 catcggtcag atcttgccta tgcttaaaaa agcaatgtct gaagctaaca aagctggcga
     2521 gaaagaacag gccaatgatg ttaataaaaa aatacaattg tataccaagc aacttaatac
     2581 cttaaaaagt gcagcgtcaa aatctaaggt tacaaaacaa cccaaagaag aagaggagga
     2641 agaaacacct caaaaaaaac ctgcccctaa aaaagtaaag aaaaaagagg aggaggcaga
     2701 agaggaaaag cctaaaaaga accctgcgaa aaaaacgcca aaaaaagaga cgtctgaaga
     2761 gtctgaggga aataatgccc ccaaaaaacc cacagtaaaa caaccgccgc ccacacaaaa
     2821 gcctgggacc caaaagccag ggacacaaaa tccagggaca caaaagcctg gtacacaaaa
     2881 tcctcccaag gaggaggcag ccaaaaaaaa tactcaaaat ccgcctaatc cagcggaatt
     2941 aaattcactt cttaccgacg ttggaaaaaa gaaaaatctt cttaaaacac tcacaaaaaa
     3001 ccccattgaa gagaaaaaag taacccaaga gcttgtgaca cttttgaaaa aggtcatgtc
     3061 ggtggccacg aaactcgaga aaaaagatat tgcgaatagt gccaacaagg atattcaaat
     3121 ttatacaaag cgtatcaaag aacttgatca aaaagccccg ccgcccacac aaaagcctgg
     3181 ggcgaatccg gcggtggcca gaattcaaca
//
LOCUS       DAIAHG010000133         3196 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_133_length_3196_cov_2.506893, whole genome shotgun sequence.
ACCESSION   DAIAHG010000133 DAIAHG010000000
VERSION     DAIAHG010000133.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133_length_3196_cov_2.506893"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          870 a          599 c          665 g         1062 t
ORIGIN      
        1 tcataaaggg atttcgcaat aatcccaaac gcatcataca gaagaaggtc ttctccattc
       61 tcatcgcgtt tggcaaccag ggaatctgtt ttactcaata taccaacccc agcataggat
      121 tctaagacat caaattgtgg aaaagtctcg gcgggagaaa agcttgattc aggcttatgg
      181 gtcgtgaaaa ttgaatagga gatctttgtc acagggtgta tccagcttac ttcccccgta
      241 aacccttgga ggagaatgcg gatagggtcg tcctgcttct ctggccaatt aacaactttt
      301 tttgatcgat tgacccgtgg tgtgctgaca aattcaatct tccagttatg tttatcaccc
      361 gtaatcacag gccaataacc ttctatggtt tttaatttat aaagtgtatg ctcatcgtga
      421 tgttgttctt caaaaagttc tacaccaacg ggttttttat caattttttg aagggcatgc
      481 ttaccataaa aaaagagcca aaaatcatta tataattgaa tgagggcgac accgggccgt
      541 tttttaaaat gaaagatata tgtattatca aggacttcaa gaaaaggtaa agtctcggcc
      601 acttgccctc cgggggcgga ggatttctcc gtattgcttt tttggcttac accttgcttg
      661 tcaatttctg ttgtgaggat gttcatggtg ttgatggcat caaggtctgc tttgatgctc
      721 ttaaattcat cattttcctt gggttgttta gcggcatctt tcttaccctt tgagggaagc
      781 atttttttct tggggggggg cgtttttaag cgtgatggtt ttttttgtgc cttgtgtttt
      841 tgcacgggtg tctttgcttt tgtttctgcg aaaacaacct gggaggtcat tcccgcaaaa
      901 agcattaaaa aaaatatttt aaggacgatg cgcgccaaag attaagggat caaaaaattt
      961 acttttttta ttataggaca aaggcgttag gcaaaaataa attttttatc tttaggataa
     1021 aattatagtt atcaattcat ttttcaaaaa tgctcaaaaa aaccattctc tttcttgtgc
     1081 ctttaatgat tttgggtgcg tcctttgtcg gtatttacat cacccaagaa agtgagaggc
     1141 ttagccttgg gttacaaacg gttattttta ccgcgctttc tgggctttca aatctttttc
     1201 tggcgtttgt agccttttgg caattacctg ctattgagaa aacaaacaag gcttccgaaa
     1261 aaacaacacg ggggcaattt cttatggaac tttataagga atggatgcaa gaagatgtcc
     1321 ttgctgcacg gtgttttttg catagtttag cgctgactta taaaaatgat ccctctaaaa
     1381 ttgctaagcg tattggtcaa agtgttttgg tcctttctga ggacaaggac gctaagcata
     1441 ttcgcatttt ttctcagatt gtctcactcc ttgaattttt agaaacactg ggcacccttt
     1501 atcagcacgg ccatattgaa ttaattgaaa ttgagcaatt gtttggcgga tccattgagc
     1561 gctactatag ttaccttagt gattatatcc atcatcgtcg tatccttggg tcgccttcag
     1621 gagttcattt cttaagtgat cagctccttt atgcaagact tgatgatctt gtcctggatt
     1681 tgaaaaatat tcatcattct taatgattaa caaaaagaaa aatcttaaca aatattaacg
     1741 gtttgttaag atttgaagcg ccatcctgag tactaaagga aggagcaaat atgctgacgt
     1801 attcaacggt tttagacact cttaaacgat atatccccaa aaaagccatt tattttagtg
     1861 ctcgccaaga taaacaaaag tcctttttgc agggtgatat ggatctcaaa agtctagata
     1921 tcacctttcc gtggcaccgt cttgatatta ttttaagcac agtggggtta aatctttttg
     1981 ctgtggtgat cccgattctt attttacagc tttatgaccg cattatccct gatcatgcga
     2041 tcagcaccct gacccttctt ggcattgggg taggtgttat catcattttt gaggggattc
     2101 tacgcgttgc acgtagctat atcctggggt ggcttgggca ccgctttgat tacaatacga
     2161 caaaaagtgt ttttaatcat cttttgggag gaaatttgta tagctttaac aagatgggga
     2221 ttggggagca tgtcgaaaat gttgaaagta ttcttatttt gaaggaattt ttggcagggc
     2281 aggggctcct cgttttgctg gatcttccct ttgttctctt ttttcttatc ctcatcagtt
     2341 attttggtgg acatgtcttg gggattgcgg ccgtaacgat cttaatttta tttgtctttt
     2401 cagcattaca tgtggggaaa aaacttcata acgccctttt ggaaagacag gagctggggg
     2461 accgtaaata caattttttg attgagattc tcaacaatca ctacactgtc aaagctatgg
     2521 ggatggaaga gctttttatg cgtcgttttg aacgtatcaa ccttcagact tcctatgttg
     2581 aatataagct gaacacacac agtagtgaag ccagggatgt gggatcaacc ttttccagta
     2641 ttctctttgg aacgattata tttgtctcag gctacggcgt tattcataac gatctatcgc
     2701 caggagccat ggcagcatgc ctttttttga gtaatcgttt aatccttccc cttcaaatgg
     2761 gtctttccat gtggacacgc tttcagcatt ttaaaattgc aaaaagccgg attaaaagca
     2821 ttttttctat taaatctgaa aaaaaccagg gcattaaaaa ggaagttttg gggcggctta
     2881 cactggaaga tattagtttt tcctatgagg gcacaaatcg tgaacttttt aaaaatgtaa
     2941 acctagacat tacccctggg gagttcgtca caattgttgg ggaaagtggt gcagggaaaa
     3001 ccacactttc acaaattatg attggaaact taaatcaaac atcgggtcat gtccttattg
     3061 atggattttc cgtgcctgag ctcaatatgc cccatttccg cacgcaaatt gcctatctct
     3121 ctcccaaagg agaaattttc aagggtacgg ttatggaaaa tttaaccttt ttctcccgat
     3181 ttaatcatga ggatta
//
LOCUS       DAIAHG010000134         3177 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_134_length_3177_cov_9.348065, whole genome shotgun sequence.
ACCESSION   DAIAHG010000134 DAIAHG010000000
VERSION     DAIAHG010000134.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134_length_3177_cov_9.348065"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          908 a          650 c          701 g          918 t
ORIGIN      
        1 tacttttgca attgcaattt ttccgataat tttcatgaaa tttcagattt tttaggcaaa
       61 attggagtat tttttgtggg ttttcatgaa aattcggtaa tttttcacga aaaaaagaaa
      121 taattttgct ttttttgtct attttttgct tttttgaaag atttttcttg attttttaac
      181 gcaatcttaa cttttgaggc ctaatctgaa ggtaattgaa gcaattgcga gctttttggg
      241 cggttttttg gccgttttga agggctttgg gggcattttg cgctgtcgac ggcgcgcttc
      301 aaggacactt tggagggctg ataacgagcg tttccggtgg ttttttgtta aaattttaac
      361 tttcttgcgg gagagacagg ggctttttgc gcccaacccc caagaaagac tggggacggt
      421 gggccgcccc gccctagggc cacctagggt ggtagggtgg gggtgcgaaa attaactttt
      481 tgttaaggtg tgtgaataaa aggttaatgt tttaacaaaa aaatctttcg ccctttgaag
      541 aaaatttaag gcgtcttgcc atttaacgca gcgatcagcg cgtttttttc gccgaaaaag
      601 accgtttcaa cgcataaaat cgccaaaaaa ccgcccataa gcccaaaaaa tggcgttata
      661 tccatgaaaa ttggcgctaa aatcctaaaa atgagcctaa tattgtgcat aatgcgttaa
      721 aaaagacctt ttttgcatga aaaacacaca aaatagcgaa aaaaatagga ttttccaagg
      781 aaaatctagg ggcgcgctga taagagaaat ttttaaaaaa atcttcgcaa aaatacaact
      841 taccccctct ttttgatcga tttttgtgaa aaattcacct tataccaggt gttttaagaa
      901 aaatatcatg aaaataggta taaaattttg tctgatcaac ctcgctaaaa catcaaaagc
      961 ctctccttgc catttatctg cccactcagt ggaacgggtg tttttgtaaa gatgcttaga
     1021 gaagagtcct tgacttttta agaaaacagc gatatcttcg tgtgaaaaat agttcgtttt
     1081 aacccaagat agatagaaaa aattatggcc aatcatactc attctaaaaa agccgttcgt
     1141 caaatcgaga agcgcactgc gattaataaa aatcgtatga gccgcatccg tacatttata
     1201 cgtggtgttg aggagctcat tcttgctaaa aaacccgttg aggcggctga ggcgtttaaa
     1261 tcagctcaaa aagagcttct tcgtggcgtg acaaaggggc ttctgcacaa aaatatggcg
     1321 tcccgtaaga tctcgaggct tgcccacgca atcaaagcgc ttaacgctta gtctttaatt
     1381 tacgcaaaga ggccttttgt gaccacgccc attcaaacag atgttgtgat tattggcgcg
     1441 ggtccagtgg gcctttttgc tatttttgaa tgtggcatga gcaagcttcg gtgccatgtt
     1501 gtggatacgt tagatatggt gggcgggcaa tgttcggctc tttacccaga aaaacctatc
     1561 tatgatattc cggcccaccc ttcgattaca ggtcaagaac ttgtggatca tttgctggct
     1621 caggccaagc cctttcatcc tgttttccat ctttcccaac aagttgtaac cctcgacgtt
     1681 ttagaaaaag aacaccatga ttcctatgga ttttatctta agacctcggt aggtacggaa
     1741 ttttatacgc gggctgtgat cattgcggcg ggtgtagggg cgtttggtcc gaatcgtcca
     1801 cctcttgaaa atcttgaggc ttttgaggga aaaagtgttt tttactacgt gaaatccaag
     1861 gctgattatg ccgataagcg agttgtgatt gctgggggag gcgactctgc cgtggattgg
     1921 gccctttctc tggttgatgt ggcaacgtct gtgtcggttg tgcaccgtcg ctctaaattt
     1981 cgtgcaaccc ccgattcaga ggaaaaattg cacaaacttg ctgagagtgg gaaaataaac
     2041 cttgtgactc cttatcagct ttcaggcctc aaaggtcatg acggtcagtt gagcgaggtg
     2101 attgtcgaga cactttcagg cgaaaaacgc gctcttgagg cggatgtatt gctgcctttt
     2161 ttcggcctct caatgaatct tggtcctatc ttagaatggg ggctagggct tgaaaaacaa
     2221 aatatttctg tggatcaagc gacagcaaaa acaagtattc ctggtatttt tgccattggc
     2281 gatgtcgcaa catatgagca caaacaaaaa ttaattcttt gtggctttgc agaagctgca
     2341 caggcatccc acgccattcg caaaatgctt tatcctcacg aggtcgtgca tttcgaatac
     2401 tccacaacaa aaggcgtccc ccaaagttct tccacggctg cttaaaatct ttaggaaata
     2461 ctctattaaa tttttatatg gctcagctta tcaacccttc gttggtggcg ttctcctcca
     2521 tcaaaagggg tttttaaaaa agcatctacg cacttttctg cctgagttat accgataagg
     2581 cggctgccta aacataaaac attcgcgtca ttatgctgac gggaaagctc tgcggaaact
     2641 acttcgtcca caagagccgc acgcagatgg gcatgacgat tcgcagccat actaatccca
     2701 ataccagatc cgcagactaa aataccccag gcgactggat tattttttaa cgcatccgcc
     2761 attttttgtg caatatcagg ataatcaacg ctctcggggc ttgaggtgcc gagatcaatc
     2821 acggtatgcc cttgagcttt gctataatct cttaaatgat ctttgagcgc gtagccggcg
     2881 tggtcagcgg caaaataaat cgttttcatg actttttttt ccttcgtaga taagctgtga
     2941 ttaaaatacc taagcaaata ataccgatga ggatgaaaag aaaatatttc tggataaggt
     3001 gaaaactgtg gagaacatgc tcgataattt caccaaaaag aaaacctaag ccacagctca
     3061 caattgtcca aacaacagcg gatataaagt taagcggtat aaagcgccaa gggggtatgc
     3121 ccgctgtgcc aatcacaata gggctgacaa tgcgaatgcc atagataaaa cgaaaaa
//
LOCUS       DAIAHG010000135         3173 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_135_length_3173_cov_3.566860, whole genome shotgun sequence.
ACCESSION   DAIAHG010000135 DAIAHG010000000
VERSION     DAIAHG010000135.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135_length_3173_cov_3.566860"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          797 a          673 c          656 g         1047 t
ORIGIN      
        1 tcacaggtac gagtcctgta gtgcccacca catccctcta gccatctatt ttagagactc
       61 tcttcaaatg cgtggcaatt cttctgcaat aaaaaatgtc attgatatta atagtgtcta
      121 tcaagacgcg tattgtcagt ctctcgaaat agcatatggt tcgggcatga tgtcagaggg
      181 aggcattgaa ggcattgatc tgatgttcga ggggattgat tttacgcata aaagcgctct
      241 tgattttggt tcaggccttg ggggaattgc tttttacctc gctgaaaatt acaacgcaca
      301 ggttgttggc gtggaaatta atgagaccat gattgagaca gcacaaaaga aaacgcctgt
      361 ttcactcaaa gataaagttt cttttgttca cagccctgac ggtcagtctt tgccttttca
      421 agaggctact tttgatattg tttatagtcg aggcgttctt gtgcatctca cgcgccagca
      481 aaaacaaggt gtttttgagg aactttttcg tgtcctgaaa aaaggaggca cccttgtgat
      541 tcacgattgg ctaagtccaa aagatgatgt gtgggggcac actattcaag agttaatcgc
      601 atctgaaaaa tttcctctct atgccctttc tccgcaatct tatcgagaag atttagagcg
      661 agcgggtttc acagatctta gattgagtga tcactctgac attttttcga aatataattt
      721 tgatgtggcc aaaaatcttg agaaggatgc tatacgggaa gaatttttaa aactctttga
      781 tgcagaaacc cttttatccc ataaaaaagg ctatgaaaat atcgccacat ccatgcaaat
      841 gggggaggtg ataagcggtc tttttgttgc ccataaatca ctttaggcct gctgttctca
      901 ttccctaaag caaatgacga tcaaaaagtt ctttaatcct tgacaaaatc atttttttca
      961 gccattttga gagaatgaag agagtttgtt tgacacacat cgtcgtcgtt tctattattg
     1021 tcggctaatg ccgaccgatc ttctttaccc tcatttttat cgcccttttc tttcaacctt
     1081 atcaaaaggt ctttagttta tgttaaaaac ggttttaacc gctggattcg ccatgttctc
     1141 tatgtttttt ggctccggta accttgtctt tcccttgctt ttagggacac aaacccttag
     1201 tcatagttta tacgccaccc caggtttttt attaaccgcc gtactcgtac cctttcttgg
     1261 cctcattgca attattgcgt ttgacggtga cagagaaaaa ttttttgcaa cgctcggcaa
     1321 ccttccaggg tttattttag cctttctcat tcttgctctt attggccctt ttggcgttat
     1381 tccgcgctgt attatagtgg cttatggtgg tctgcagctt gtttggccga cgctttcttt
     1441 tcctcttttt agcgctttct tttgtctgct cacaggagct ctgatctggc gatccaacaa
     1501 agttgttagc attattggcc ttgtgcttac cccttttaag cttgggggaa ttgtgattct
     1561 tatccttttc ggtctctatt ttgcagcgcc tgaagaaagc agtctcatac cctcaatcgg
     1621 tgcctttaag cttggggcga gcatgggcta tcaaaccctc gatcttatgg cagggttttt
     1681 cttttctgca acgattgtgg cctatttacg ggatcatctc aaaaataagt ctgataaaaa
     1741 acaactgctt ctctcaagtt ttggcgcatc gatcattggt gcccttttgc tcacgctcat
     1801 ttacattggg tttataaaac taggggccag ctatgctccc catcttcaaa gtgcaagtcc
     1861 tgaagcactt ttagcagcga ttgctggcct gaccatgggg aaatatgctc ttgtgattgt
     1921 cagtacaaca ctcgctgtgt cttgcctagc aacagctgtg attttatcga atttattcgc
     1981 agaatttttg aaaaaagaca ttgccgaaga gcgccttcac attaatttgc ctcatgccgc
     2041 gagcattatt ttaacaattg gcgttacatt tttgattgct cagttggggt ttgctaaaat
     2101 ttgccaagtc cttggaacta ttttaacggt gacctatccc gcactcatcg cattagcaat
     2161 tcatcacata ttgcgatttt ggtatgattt taactacagc aagatgattt tctggggcgt
     2221 tctcattcta tctatcgcta tgacttattt ttagaccaga agaaaactct gccccttgag
     2281 tatttttata ctggggggca gagtttttta gcgttagctc tctctttttt tgcaggacgt
     2341 gcagggtttt accgccgcac tttcctctcc atcctcaccc agtccaaaag caccgccttc
     2401 attaatattg gaagtcgtag ctgccgcagg ctttacaggg cgtgtgcaag gaccatttgt
     2461 ttcatcacca cacgcctttt catccccgag ctgaaaaaat tcaggatcat acgcatagta
     2521 aatagccgga tcagggtctt ttgggtgatt ccatttatat ccttcatatc catagccaag
     2581 ggcaagtgtt acaagaattg aaatcgtggt gggttctggc agaaacattc aaaagctcct
     2641 tatcattgaa tgacgtcatt ttagctgact gtagcatagg aaagtttaag aatcagtaaa
     2701 gtattatcga ttatttttaa aaaattctcg ccgaaaaatt aggaacacag ttatcaagag
     2761 agaatcatga ttggacaact gtggttcaga agtggtattt aagaaaggaa gatacacgat
     2821 tatcacttct ttttattatt taagtaggtt ttccatggat acaacagcgc ttcaagccgc
     2881 cactgttcaa acacatcaag cagcacagac cgcccttaat caagtggcaa cgcagacagg
     2941 cgtttcgatt tgggatcttt tttggggagc aacccctgtt gtcaaacttg tgatgattgt
     3001 gcttttggtc gcttcttttt ggtcgtggac gattattttt aataaagtga ttaagcttcg
     3061 caaacttcac ggacacgctg cgcatttcga agaatctttt tggtcagggg gatcccttga
     3121 tgatctttat gaccggctgc atggcaagtc ttatgatcct atgagtctta ttt
//
LOCUS       DAIAHG010000136         3153 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_136_length_3153_cov_3.124837, whole genome shotgun sequence.
ACCESSION   DAIAHG010000136 DAIAHG010000000
VERSION     DAIAHG010000136.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136_length_3153_cov_3.124837"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          970 a          687 c          635 g          861 t
ORIGIN      
        1 caacagccaa cagccaacag ccaacagcca acaagtttag attactcttt ttaaacctga
       61 tggctttttc tgccctttct ctccctaccc tggcatccga taatgaaaat gagcgcgaga
      121 agaaaccaga agtaataggg ataacactca tcacaattcc tcaaaaatat gaaacgaaaa
      181 aagtaagtga tgaggactat gaaaaactcc tcggtcatgc caaccataac gtgaaaaatt
      241 tacgcaccct tatggatcaa aataaagagt gggatactaa gaatatgcaa gggcgcctca
      301 aaaaccttga aaacctatac agtgttcttt ttcaaggtta taagggagaa aaccttgagg
      361 gaaaaaaact gaatatgaca gatgagcaaa aattcaatgt ttatataaac gtgcttcaag
      421 agatggagca tctggattat tcattacttg agcagtcccc agaatccttc agcactctct
      481 tcaaaaaaat aaaagaggaa tcgggagata cgtgggcccc cctcaaaagt gaacatacaa
      541 attgtatagt ttcgtaagct tagaatattt taaggaacat taccaggtgc gctgcggaca
      601 agatcgctga agaatgaaaa gctgataaat gaaaagtgga aggtttgcct aacccataca
      661 taaaaatgct gcggctgctt gctttcgcac ctgaccgggt tcacacaact gtctgtctag
      721 gaaaccttcc aaagcgatct taacaaaacc tttggaatat tactagtgat cactgagtat
      781 ttttgcttga aagtgtcata aaaaatatca atatatacca tgactacatt ataaaaaatg
      841 ccctcgtttt tctagtaaaa tgctttagca tgctttataa gtagagtgaa ttgaaaaaaa
      901 ttattaagaa atgatgacga aactattttc tatcgctttt tgccttgctt tgagttctct
      961 ttcttctggc gttgaggcaa cgtttgcccc ctcaatctcc cgctatggta cacccaaata
     1021 tgatctcaca aagcccctta cgctggatta tttaaatgcc tcggccccca agggtgggaa
     1081 aattcgcatc ggcactgtgg gtacctttga ttctttgaat atgttcactg tcagcggcgt
     1141 cgcagtagaa ggtttaacgc acacccatgc accgctgatg cgccgactcc ctggcgatcc
     1201 ctttagccgc tatgcttgta tcgccgaaag cgctgaagtc gcccctgatt tctcaaaagt
     1261 cacttttaaa atgaataaac aagcaacatt tcacgacgga agtcctatta cagcagagga
     1321 tattgctttt acggtgaagc ttttaatgga taaaggttgg ccacgttata agcaatccta
     1381 tggtaaaatt gcaaatatta ttataaccga tccccacacg atcacgtttg aactaaaacc
     1441 caacgataaa ggtgagtttg atcctgagct ccctctcatt ttgtcgcttc ttcagccgct
     1501 gcataaaaaa tccttggaaa agagtgattt tacagcaact catatgacgc ctctcatagg
     1561 atcgggccct tataaaatta aaacagtcga gcccggccat catattgtct atgagcgcat
     1621 caaaaatcat tggacagaaa gcctttcgac aaacaaggga gcctttaact ttgatgagat
     1681 cgagattttt tatactaaaa atacagcgac tcatttccaa gagtttctca ccgggaacct
     1741 cgatatttat tttgagcaag atcccaatca atggaacaca cgttatgata ttcctcaggt
     1801 aaacgatggg cgcattacgc ggattgcact tgaacaccaa atgcccgttg tcgtgcgcac
     1861 tcttgctttt aatatgaaaa atcctctttt tgagcatctt aaagtgcgcc aagcccttga
     1921 tcttgctttt gattttgaaa cgcttaataa aatggttttt tacggcagcc ttaaacgcgt
     1981 taaaagtctt tttgagaaca cacccctggc acatcagggc gctgcgaatg atctcgagcg
     2041 acgtctttta tccccgcata tggtcatgat tcaagatcaa atcagcaaag gtgagttgcc
     2101 agaaaatatc cttgaagaac cttatattcc cccccacaca aacggtgacg gcaatcaacg
     2161 tgataatctt gcaaaggcgg atgagctctt aaaacaagca ggatggacta tcaaaaatgg
     2221 caggcgcgtt aatgccaaag gagaggtttt tacttttgag ttcctgatta aagatccccg
     2281 ttttgaaaaa attgctctgg gttttcaacg tagtcttaaa gctcttggga ttcatcttaa
     2341 agtacgcctt gtggataccg tccaatacga aggccgtgtc acagagcgca actgggatat
     2401 gattgtccac tcttggtcca atacattatc gcccggtgta gagcaagcct attattttag
     2461 tcgccacacg gctgatatca aaggaagcag taattatatt ggcgtgaaag acccactcat
     2521 cgaagccctt gccgtggatc tgagccgatc gaaaacaagt gatgagctta cggcctatgt
     2581 gcataatctt gaccgttttg tcatgcgcaa aaattatatg attccgctca gctatagcaa
     2641 tacatcctac acggcttact atggcaaacg cattgctttt ccaaagccta cacctttgac
     2701 aggaattaat gtgattgaaa caggttgggc cattcagggg gagacagcac ctgaaacttc
     2761 tgatcatacg acgcatacgg gtttatggaa tcgtttaatc gtgtggatca aaagcttttt
     2821 ttaagagcat ttttgcacta gaattcagga gtcacactta aaagagaaaa tactcctgcc
     2881 cccttttcaa aaagatctgt tgagtgacat aaaaacaaaa gggccgctct ctctttgtga
     2941 ggttatggag cgatcaaata cctattacta tgccaataaa tctatttttg gcgccgaggg
     3001 ggattttatc acagcgcctg aaatctcgca gatttttggc gagatggttg gcatttggct
     3061 actcttgcaa tgtgaaaaat atgcaacaac aacgccgcac attattgagc ttggaccggg
     3121 acgcggcacc ctcatgaagg atattttgcg tgt
//
LOCUS       DAIAHG010000137         3130 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_137_length_3130_cov_2.760236, whole genome shotgun sequence.
ACCESSION   DAIAHG010000137 DAIAHG010000000
VERSION     DAIAHG010000137.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137_length_3130_cov_2.760236"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1021 a          673 c          603 g          833 t
ORIGIN      
        1 agcgcaaaag aagacgccgt tggataacga aaacttatat tttgacacaa aatatgtcct
       61 ttaagcgtgg gtcttgtaac agatttagga gcatagtgtt ctgttggtag atccatcatt
      121 tgattcaact gccgcatcgt cgacagcgtc agatcgaact gacttaaaaa cataaaacct
      181 gcttgaaacg gggaaagagc acgccagatc aacaacacaa ccgccacaag acttccggtg
      241 gtcatatcac catccatcac gcgcactgct ccccaaagga tggtaatcac acccgctatt
      301 ttaacaagaa tttgggaaag attcgccgca taggaggata tgatctcact gttttccaaa
      361 gatttgctgt tgttataggt tttttcctca aagcgttttg cgaaaacaga ttcgccccct
      421 aagtacttca acgtattaat tttactaaaa cactccagca taaatgacaa tttctcattt
      481 tgcgtaaaac tgctcaaacg tgtgctcccc cgaatcatgg gaaaaagaat aaagccaaga
      541 ataataaaaa gagcaagaag gacaatcgga acaatcacaa gaggtccgcc caaaatgcca
      601 agaataataa taaaaaagat gacaaaaggc ccttcgagaa tcagttgtgc gagaggagat
      661 gtgaaaaaat ctcttaaact atcaaattgt ttaatccgcg caatttgatc ttgaacaggt
      721 gcactttcaa tgaatgtata gggtaaacgt aaaatttttt gcaccaccga caaactaatg
      781 atcatattga tacgcgcccc tgcaaaagcc agggatttgt tttgaaaccc gtggataaaa
      841 tggtgacaca caagcgcaat aataagtcca acaagcaaat aattaagtgt cacctctgat
      901 tttgttggaa tggcccaatc aaacacagcg cgaatgaaaa aaggcaaagt cagtgcaaaa
      961 atcgcattca gcagcccaat ccaaagcagc tgtagaacct gcggtttaaa acgctgaata
     1021 atctcctcga tccatttttc tcgaatattc aacggccgcg cattggtaaa aaaagaacaa
     1081 aaactcccac tcaagttgta cccccttgga agtattgtat gctcttttgt gtcgagatta
     1141 atgaaagaca ccattgcggt atcgactttt gtcacaataa aagttccagt ctttgtgata
     1201 aaaataccag ggtgctcagg cattctttga agatttgttt ttttgacctg actggcatag
     1261 cctaagttgg caaaaaattc gcgcaaatcg tcgaggctta gttttgacac aagatgcggc
     1321 atggcctcga gaataacgcg atcatccccc ctaaacccaa ggctttgaat cagatgtgga
     1381 atagcgctga gaaaattata gtcctcactc attaggcagc ctttctgtca ttttttgatc
     1441 ttttatctcg aatgtgcgat cggcataacg cgacacgctg ggtctgtgcg taataaaaag
     1501 aatcgttatc tttcctttta attttgacag aagagaaagc atttcaaggt caccctctat
     1561 atctaaattc gtgttcgctt catcaagaat aagaatctgt ggacctaaca aaagcgctct
     1621 cacaaggcac attctttgct gaatccccaa gggaaggtca ctgtcaatat gcacaccgat
     1681 gtttgtttga tagccataag gaagcctact cacccaccta tccaaaccta cttgacggca
     1741 aagagagtaa atatgatccg ttttttcagg cggcgagaaa aacgtcagat tatccatcac
     1801 cgttccttta aagatctctc ctttgggcgg gagatacgca acttgttttc gaaaagaagc
     1861 gatattaatg gcaccaagag gcgtttcatc aattttaatt tccccggata caggctgaat
     1921 attccctaag atcaattgag aaagtgttgt ttttccaata ccgctatccc caataataga
     1981 aataaattct cctgcgttaa tcgatgcatt gagattctca aaaagaatac gatttgtccc
     2041 ctcataccca aatgttagat ctttaatcgt aatcgctcct ttaattttct tttggacacc
     2101 ttctgtggat tcaaagggaa ttttaaagac attttgaaaa cgtttttgag caattttaaa
     2161 gtgctgaaac cgtgtccaca cagaaagccc ctgttgtaag ggttgaataa gacgattact
     2221 caaaaataga cacgcagcca tagcccctgg ggaaattttt gtatcaataa cttcaaaccc
     2281 tgcaatataa ataatcgcgc caaaaagaat attcgaaaaa acagagccaa gatccctcgc
     2341 ttcactgctg tatatattca ttctgtaatc aataaaagag ctttggagtt gaatgcgctc
     2401 gaatcgtctt aaaaaaagct cctccatccc aagagattta agcgtgtaat agttattcag
     2461 catctggatc agaaaactat atttacggtc ccctatgtcc tgacgctcta aaagagtttt
     2521 gtgcagtttt tcacccatgt ggagtgatat cacaacaaag gcaatcaaaa taatcataga
     2581 aacgatgaca atcggtttac ctccgaagta atcgatgagc acgagataaa agagaaaaaa
     2641 gggaagatcg agaaaaatca agaggctttg ccctgcgaga aattctttaa gtgtgacaag
     2701 gctttcaata ttttcaatgt gctcaccaat gcccatttta ttgagatcat gcaaattcgt
     2761 tttcattaag tgggaaaaaa cagacgtaga cgtactataa tcaaattgtt gaccaatcca
     2821 cgataagaca tacccgcgtg caatacgcag cactgattcc ataatcacgg ccacagccac
     2881 ccctacggct aaaatcgaaa gagtccccat cgcttgattg ggaataatgc gatcgtaaag
     2941 ttgaaggatc acaacgggca atacaatcgc aaacagatta agacagacgg tgctaaggat
     3001 aatatcaatc cgtttccacg gatgtatcgc ttgtggagaa gctttctcct ccttattgtt
     3061 caaaggaacg ggctcagcct catagctggc ttctgaagat ttttgtgcga ccgcttggtg
     3121 tgccataact
//
LOCUS       DAIAHG010000138         3092 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_138_length_3092_cov_3.311443, whole genome shotgun sequence.
ACCESSION   DAIAHG010000138 DAIAHG010000000
VERSION     DAIAHG010000138.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138_length_3092_cov_3.311443"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1013 a          471 c          744 g          864 t
ORIGIN      
        1 agatcttagg gaagaaaatt gctgttgggc caaaagagct ggggcaggaa ttggctgagc
       61 aactttaccc taaagtaaaa gagagaaaaa caaaaattga aaaactttta gaatcaacgc
      121 ctcatgatgt gggcgagaaa gagaatttcc tagccatcat taaggttgac ggtaaaaaac
      181 aaccccttca tgagcgggga aaactcaggc atctgacaat gaattgtacg gcgttagtgc
      241 gtaaggtctt aaaataagtg tgcctaatag tgaggctatc atggatattt tttgatcata
      301 acatctgatg tgtcaccttc aaaatagccc ctttgggagg tgtacgtgcc aatttttcta
      361 aagcctgcct tttgatagac atgctgtgca cgaggattgt catgggatgg atcaatcatg
      421 tacatggtgg ctgaggggtc aatagaatcc ttaaaaaaat ccataaaggc acagagtgtg
      481 ggtgctgcaa gcccctttcc aagataatga gtattaccaa tcataaaatc gagggcgatt
      541 gtttttcctc cttgaaaaag gtggtcaaaa aggggaatgt agggtgattt ttccgcaagg
      601 cattgctctt tgaggagctc tgaggtcatc atgagtgcaa agggatcctt gccaatgaat
      661 cctatccaat aggcatagtt gtggtcacca ttaaaataag gagagcgtgt tgtgcgtcct
      721 tccataaaat taataatgtc gaggcgatgc tctggcgcat tatcccaaaa ttccatgacg
      781 tggggctcat ccagccactg aaaaagtgtt tctttgtgct caaatgtcgc acgttcaaag
      841 gttatgggca gcgtcatctg agaaggcttt tgcgcattta tttttcacaa ctttgttttt
      901 ttattctaat gcagtgaaaa tcatttttaa aggagatcga tgatggatga tcgtagtgat
      961 gaacaaactc ttgaggagct tttggagagc tttcgcttta tgcaaaaagg taaagtttat
     1021 gaaatgccca attgggcgat agagtgagta gtttttatga tttgattttt aggcataatt
     1081 tttatatgtt ttattcataa ataattaata tataaaaaat gtttttatga ataagtttat
     1141 taaaattatt tctacctcta tgcttatggg attttcaatt tcttttgcaa tggaaaacca
     1201 gatagaagaa acgataaaag aagaaaaagg ctatttttat tggctgcact atatggaaaa
     1261 cggcaatgaa aagcaaaagg aagaggcgct taagatattg agcgagtttg aaaaaattat
     1321 tgatgtcatc aaggacgaaa gaaatctgtt taaggaaaaa aatagccgcg atattaatga
     1381 ttttacacca cgtttttctt ctaaaagtcc atcagagtct tttacgaagg aagatcagga
     1441 gtttgtgaat tttatcaaaa aaacgatttt ggaaaagggt ctcacttttt atgattttta
     1501 tgagatgatc gaccgtgtct catccacaat aaaggataaa cgtctttttt attttctaaa
     1561 gaatgcgcat aactatgata aagagtggtt tgatggactt gaagaatcta ggagagaaaa
     1621 tatctcgaga gttctagaaa aattgaaaat taaaaagaaa aattaggtga atgttttttt
     1681 aatagagggg gctgcttttg cagctctttt tttttatgtg ttgtctaaat ctttgaaaga
     1741 ggtcctcagc aatggaaaca gaattagaag tgtcgggtgt tggatttcct cccttttcag
     1801 cgcgcgggtg cgtgcaggaa ttgtatccta tggccatgac aggagatgtt tttgtccgta
     1861 cagtcaacgg cgaattggtc tatttagggg atggaagtga tgtgaagtat gggacaaata
     1921 ttaaatgtca ggacaaagat gttcccgctt ttgcgcacct ttggcggggg agtcccgtga
     1981 cagttgcgtg cattcagccg ctgctgcaac ctcttttgga ggaaaaaaca acgttgatgc
     2041 ggccttttat gaagggatcg ctccgcgcgc tgaataaaaa aggggaggtg attgatttta
     2101 aggtcgagga gaatgtcgtg tgtgtgagtg atgtcggcgg ttatgtcagt taccgtcctc
     2161 ttttgtcgat ggcgctgatg gattttaaaa tgacccacaa tgaatggggc gggaacacag
     2221 gctggagtat gacattagaa gagatttaat ttttactccc tgttaacttt tttttgctat
     2281 acctattcat gtaatcgtca tgtgcgatca tcgtaacgaa aggatattat aatgaataag
     2341 tttagtaaaa tacttgtaaa ttctatggca tttatgctat tggggggggc aattacaggg
     2401 tctgttgttg caagtgaatc ttttgctgaa aaacaagaaa agattgtcga gaaaaagata
     2461 agtgagctcc caaaggttga tcaaaagaag atgaagacat acctcgacat ggataaaaaa
     2521 ctttggaaga gaatgggtaa agatgaaaag agggaagaat acgggaaaga gattaggagt
     2581 ctcatattag acataagaaa attacaagat cgtatgcagg gcaaaaaaga aggtgaagaa
     2641 aaggataaac taaagaaaga gctaaaaaag attgtcgaca ctaaaagtaa gttagagaaa
     2701 gagcgcgcgc aatataactt agagctttta gaagaaacga ttgaacatct ttccaaagaa
     2761 tatgagatgg agcgtaagga ttacgataaa atgaatgatc tttatgccaa aataaaaggt
     2821 gaggcagaaa cggaagtggc gaaaaaatcg aaaaattaga ttaggactcg cgcctcatct
     2881 cgcgccacgt aatgtaaaaa ttgctggcga cgatgaggct tcctccaccg atgatccaaa
     2941 gacgggggat ttcggaaaaa agaagaaaac caatcgtggc ggaaaaaagc agttctgtgt
     3001 aacgaaaagg catgagccct gaggcgtccg ttgcggcaaa agcacggaag atacaaactt
     3061 ggatcatgtt tgcgccaaca cccaggaggg cc
//
LOCUS       DAIAHG010000139         3085 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_139_length_3085_cov_2.891290, whole genome shotgun sequence.
ACCESSION   DAIAHG010000139 DAIAHG010000000
VERSION     DAIAHG010000139.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139_length_3085_cov_2.891290"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          935 a          572 c          706 g          872 t
ORIGIN      
        1 aattttgggg gcgacactct caagcgtcgt tttgataaag gaaataaaag caattacctt
       61 aggttttata taggtatgat aaacatgttt catgactttg gcccgccttg aggtgtggga
      121 ttttgtgcgt gtacagcctt aagagggggg tgctgctgct cttggatttt tgctaaaaag
      181 tcatgacggg ttccaaaaaa attgacttcg ccttgttcaa gaaccagcac cttgtctaca
      241 agctgaagaa gattaagatt atgcgagaca agaatcattg ttttgtgggg taagatattt
      301 ttgagattat tgataaaacg ggcctctgtg tggatatcca tcatgcttgt gggttcatcc
      361 aggagaagga aaggggcgtc cttaataaat gctcttgcga tacacacggc ttgtttttga
      421 cctcctgaaa ggctgttccc tgaatcttta atcagcatgt tcatgccctt ggggtgatgg
      481 aaggcaaagt cgctcgcccc tgaaaaaaga agtgtgcgct cgatgtcttc tttggttgtt
      541 ttttgggaag agaggataat attctcaaga agggtgcctg aaaagagata ggtgttttgc
      601 gggacatgat tgatctttga gcgaaaatcg acagggtcaa tttgtctttg atccacgcca
      661 tcaataataa tctctccctc agtgggttca taaaacccta aaataagttt ttcaatggtt
      721 gttttgccag agccaatttt tcctaaaatc gcgattttat ccccaggatt aatctgaaaa
      781 gacacatttt taagggcggg agcgctctga tcggggtaag taaaagaaac attgcgaaat
      841 acaatcgacc ccttaagtgt tggttttgcc gtataatcaa agtcagtttt gcgctcagtg
      901 ggcatggcca taagctttga aatggatttg tacgcatccc tcacatgact aaagcgcaaa
      961 agcacggaaa cagcttgcga aaagggcgta aggcagcggc tgctcaggat tgagcatgca
     1021 ataaggccac ccattgtgag ttctcttgca ataatgaggt agacgcctgc aatgacaatg
     1081 ctaatatagc tgaggttgta aactgttgag gcaatattca cagcaagact gctaaaaaaa
     1141 ctggattttt tagattcctg tgcgctcatc tctgaaagaa cgccccattg acgctgcatt
     1201 tttgcctctg ccgtcgaggc cttaatcgtc tcgaggccga ggatagattc gacaagaaag
     1261 ttattttttt tatggaccgt tttctgagca tgttctgtgt gatggctaat cactttatgc
     1321 aaaatgtaat tggccacaag aatcaataga atcgcagtga cttcaatgga aagcaaaaga
     1381 gggcccccga taaagccaat aataaagaga aaaagaaaag caaagggaat atcaataagt
     1441 gtgacaagtg taatggagga aaaaaattca cgaatcgttt caagttcgcg cgtataactt
     1501 aacatcatgc cactggagat attatgttta tcaagtttga gggcgaatat tttttctaat
     1561 aatagattgg aaaacattaa atcagcattt ttccccgcaa aatccacgag ataacttttg
     1621 agtgttttta aaataaaatc aaagcttatt gcaattaaaa tacctgcaga caggacccaa
     1681 agcgttgaaa aagaggcatt gggaatgaca cggtcataca cattcatggc aaataaaggc
     1741 accatgagtg ccagaagatt tgtaataaaa gcggaaaaaa caacttgtgc atatattttt
     1801 ttgaaatgct tcaagacatc taaaagccaa ttatcataaa agggttcctt ttctttagag
     1861 gactcttcat ggagattcac aatgggggaa aagtgaatga tccggccgtc ataaagatca
     1921 ctgagtgcct tgagagtgag cgttgtttgg cgtggatttt caggatcaaa aacgattgcg
     1981 tgactttttt taatctcctc aagcacaaca attttattat ttttgaggat aagtaaacaa
     2041 ggccatgctt cctttattgt ggggaggtct acactttcca cgatgtgcgc ctccagctta
     2101 agacgttcac gggatatgcg ctgcataaat tcaaaatcga tggtgtcatc cacggcatag
     2161 cctgcgcgca gactttcctc tgtaatgggg tgattataaa gatccgcaat ccttccaagg
     2221 gccttgaaaa gaggatcaat agaggattcg tgaaaagagt ccgacacgat gaaccaaaac
     2281 ttaggacaac ttaccttgat ctccattatc gaatcaaaaa gtaaatattt tcttaagagg
     2341 aaaaaactca ttgttgtgat ggaatataaa aaaagattgc gcttttggtg ggcctcgcga
     2401 atgataaaag agagattccc acgcatgact gctttttaaa aaaatggatg attcactttt
     2461 aatctctacg gatgctttgc tccagcctct tgaggataat acggcgggtg cttacctttt
     2521 ttatgaacct gcttatgatg tgattaaaaa agcgcgcatg gaggatgaca gtaagctctc
     2581 gcggggggtt tgggagattg atcttaaaaa agctgagcac acagttgttg aaaaaacttg
     2641 tgtggatatt ctggcccatc agtcaaagga tttacaagtt ttggcgtggc ttgtcgaagc
     2701 gtggtttgtt ctttatggtg tatcaggggc cacaagaggc ctagaggcca ctgtgtcttt
     2761 tttggaaaaa tattggacca taatgtatcc tctcgatcca gagcatcgta ttcatatatt
     2821 tgaatggatg gatgcaatgc tgagtgcaaa gtttcatctt ttgccgctga cttcccatga
     2881 aaatacggcg cgtgcctatc attttcaaga ttaccaaaat gccaagcgtt tggacaaaga
     2941 tataaaacgc gaccccaaag gggcggcccg tcttttggag cgcgcaggaa aaagaggtga
     3001 gcccacactc gcatctttta aggaaatcta tcatcaaaca cctaaggcgt atatcaaaag
     3061 aaatctcaaa gatattcagt ccttt
//
LOCUS       DAIAHG010000140         3068 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_140_length_3068_cov_4.237044, whole genome shotgun sequence.
ACCESSION   DAIAHG010000140 DAIAHG010000000
VERSION     DAIAHG010000140.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140_length_3068_cov_4.237044"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          930 a          573 c          577 g          988 t
ORIGIN      
        1 gtgtgtataa agattttggc agtgttgagg attttgtgca ttttatccac gagatggaga
       61 aataaaaata tttattgaaa ttattaaata aaaacaaata taaaagataa tcaaaacaga
      121 attatttttt ataccatgaa tattcttcaa aagattctta ttcttacact tcttgcgatg
      181 cacagtcttt gggcaagtga atcccttagc gataatctca ctgaggagtt tgataaactc
      241 tccctcactg aggaaaaaga agtcgccccg acttttgtgg gtgagcaaac gcttctcagt
      301 ttcgttaaag atcaatttaa tgagaacgag ggttttagta atgacacacg cacaaaagat
      361 atcaacggct tattcacaca aaacctctct tatatacgcc acacgctcaa tcagcaaatt
      421 gagacaaagg agcgaaaaga aaacgacgtc aaacatatgc tgaaaaatat caaggatatg
      481 gaggattttg taagcgaaaa tgctgaaaat gaaatgtatc ttaatttgag agattacggt
      541 gcaatctgtg atttcttgtc cgtcacatta agtcttgatt gcaggcaaaa gctttctgaa
      601 agccgtttta gagcgaagag tttgtacggt gagatagcag agcatcaatg ggattattgt
      661 gaaaattggc aagagctttt ttcctggaat gcctttaaac aagtatggga aaaagcacaa
      721 aaagatatcc ccgaacaatt cgcaaaaacc cctgattatt tcctttatga aataggaggt
      781 gctcaatggc ataattggct ttatctgatt acccaaacca tttttattcc tgtgattaat
      841 tttgacggca ttgtagatct taaaacaatg tcaaaaggta tggctttttt tgataacaat
      901 aatagggttc acagtgctgt tttcaatggc tgtgctgtga aaaaagaagt tgactatgac
      961 aacttattaa agcagcacaa atataatcct aatttcgtac tctgtgcaaa gaacctgtgg
     1021 gaacatgata tggcgcacct tcatcgtgcg ggctggatgt tgctgaatca accacgcgaa
     1081 atttttgaaa aaagcaaaga aaaatacttt gattattatg caaaaaccgt ggaaatggct
     1141 tattttctga aatatttaga tgatcatgaa aaagagattg aatatcttga tgatagttat
     1201 gactggtttt ttaatgagtt tcatgaaaga catccaagct atcatcaccg cctgagtgaa
     1261 tttaaaagat cttataatga taaagaacgc acccgtacca cttcttggaa tagcgcaggc
     1321 ttcgaaatca tgaaaattct tagtattgtt tttaatgaga aggaaaaaga ataaaattta
     1381 accgcccatt gtacgcgttg tgagctcgtt gattttctca acagccacac gcagggcttg
     1441 ggacacaatc tccatatgtg tgctcagttc ttggatggac atggccgttt tttcaacatt
     1501 tcccgtacct aggatttgtg ctgttatatt tttctctgcg tctgcacccg attgctttaa
     1561 actatcgagt gtagctttgg gaaaagatga caattccgat ttaaacccat gcggtgctga
     1621 ggattttatt cctacctcac cctccccgcc caagggatcc ttagaagaat ttactcccga
     1681 gctaccaagt tttgccgcat ttgtataact ttgaagtgca cttacattca taagaaactc
     1741 tctctttttc tattttttat cctagccacg gctaccacgc agtaaactaa tcgcatcttg
     1801 ctgcatcgca agggcctttt catacgcctt aaggcttgcc tcatacccac gtttggcttc
     1861 catcgcatcc gtcatttcaa tcggcggctt tacattggac tcttttacat aaccattttc
     1921 atcagcgcca ggatccccag ggctataaat aagcgggaaa ggcgttgtat cacgatcaat
     1981 ttttcgaact ttcacaacct caaccccctt ttttcggtcc atttctgatg taaaactaat
     2041 gacttttcgc tgatacggat tttctcccgg agaaggtgcg cgcgtccccg catttgcaag
     2101 attttgagcc gccaccaaaa gacgctgtgt ttgcacctgc atccctgaca aggccacttg
     2161 taaaactttt tgaagcgacg aaggacctat cataaatacc ccctaactac gtcctatggc
     2221 cacacgaatc atctcgtgat agcgtttgta aatgtgaatc agggattgat aatccatcgc
     2281 aagagtttga agattcaaga cctctgcctc acgattaatt tgctcccctg tattaatgac
     2341 atgttcttcg ttcagattca tctcaagacc atgcgccgtt tttttgacga ctttttcaaa
     2401 aggcttaagc tctttttttc gcgcctcagg aatatcagcc tttgccacat tttgagagga
     2461 ttgaagtgca gaagcctgta aatagctcat tttatgaatt agtaatttta aaagatttgt
     2521 attaaaaagt ccgagatcat tcgtcatagc taatttgatt aaattttaat aaatttctac
     2581 caacaagcta ttttcacccc ataaacctta ataaaaagtt aattttcaga gttagtttaa
     2641 aaaattaatc atttaaagaa ataaagtagg ttgggataat tttttgttga atttgtactc
     2701 tgtaggcaaa aatcgccctt aaaaatcaga aaaatgggcc atgcgcacaa gaaatttatg
     2761 attttttcat ttcattttct agagatgaag ttttattttt tcagcgtaat ttttgtgttt
     2821 acatgttaat ttttcaattt tgaccgatat tttttgagtt tttcacgaaa tattcgatgg
     2881 aaaggatgct tttttgggta aaatttgtgg tttttgagta ttttagtgac atttcgcagg
     2941 agttttccat tttttgacca tgattttgtg gtttttcatg taaaaaaggg cttttttggc
     3001 gattttatgc gttgaaacgg tctttttcgg cgaaaaaaac gcgctgatcg ctgcgttaaa
     3061 tggcaaga
//
LOCUS       DAIAHG010000141         3052 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_141_length_3052_cov_3.360000, whole genome shotgun sequence.
ACCESSION   DAIAHG010000141 DAIAHG010000000
VERSION     DAIAHG010000141.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141_length_3052_cov_3.360000"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          869 a          597 c          690 g          896 t
ORIGIN      
        1 accaagcttt tcatgaatct ttgatatttc taagaaattc ttcagaccta gtgactctaa
       61 tacacccttt tcgatttttt cagcagaagg aaagaggtaa attttagatt tttccctttc
      121 ctgaagatct tcagacgcca aaagaggatc aatgttcaaa acctgcacac ataaaaatag
      181 aaatgcgaac attctaaatg catgtttcat aaaaaatttc ccttttttga gtttcactat
      241 gataatcttt gcccatcaga ttttggagtt caaggacatt tatgacaatt ttgactgtta
      301 ttcccgcgcg tatggcggct gtgcgtttgc ccaataaacc ccttgcggat attcatggca
      361 ttcccatgat tgtgcgcgtg atgcagcaag cgatggcagc caatattggt cctgtaattg
      421 tggcgtgtga cggtgaagcg attaaagagg ctgtggaggc ccatggcggt gtggctgtga
      481 tgacagatcc agaacttcct tcgggaacgg accgtgtgca tgcagccgca gaaatttttg
      541 atcccaaggg ggcatatcaa ttgattatta atgttcaagg cgaccagcct aatttaaacc
      601 cggatgacct taaaatgatg gcgactttta tgtctgagag tgatcatgat atgacaacca
      661 ttgcagcaaa atcccttgat gagggggatc gtaccaatat aaatgttgta aaaattgcga
      721 tgaaaagtgt gcctataggc tcaaaaacac aagcacatta tttctcccgc tcgagtatcc
      781 ccagtgaggg aaaagatctt tatcatcata ttggcgtgta tggctacaag cgcgatgttt
      841 taaaaagatt tgtgagtctt aaaccttctt accttgagca aagtgagcgc ctagagcagt
      901 tgcgcgccct tgaagatggg atgcgtattg atgttgtggt tgtcaattcc attcctcaaa
      961 gtgtggatac ccccgaagat cttgaaaaag tgaaaaaact aatcgcggct taatgcttca
     1021 aaaagagata ttttttcggt gggttgttag tgtttagagc agaatgcatg cttaagacat
     1081 gcaaattcta gacttttagc gttaagaaaa ggtagtgtta tagaaaaata acatcacgaa
     1141 aatatgtatg accattaaag ttcgttttgc accgtcgccc acagggtatc tccatattgg
     1201 aaatattcgc gctgctctta ttaattggct gtttgccaaa aaacaccaag gacatttctt
     1261 gctgcgcatg gatgatacgg acctcgagcg ttctaagccg gagtatgagc ttgctattct
     1321 tgaagacctt gagtggcttg gcctaacgca ccaaagtttt tttaagcaat ccgagcggtt
     1381 tgatcgctat aaagaaatga tgcagatgct gattgaaaaa ggccggctct atccctgtta
     1441 tgaaacgcct gaagagcttg aatttaagcg caagcggcaa ttgggacgag gcgagccccc
     1501 gcgctatgac cgtgctgctt tagcgctaac agatgatgaa aaaaaagctt ttgaaaaaga
     1561 aggtcgccgc ccccattatc gttttctgct tgaggataag gatgtttcgt ggaatgatct
     1621 tattcgtggg cctgtgcatt ttggccctgg gacgcttagt gatcctgtgc ttgtgcgtgc
     1681 agatggggct tatctttata cactgacgtc tgttgtggat gatgtggata cagacattac
     1741 tcatatcctc agaggcgagg accatgtaac gaatacagcc gtccaagtgc agctttttga
     1801 agctttgggg cgcgatccaa aaacgctttc ttttggacat acgacgttgc ttgttgataa
     1861 agacggtagt ggcctttcaa agcgcctggg aagtctgagt gtgggtgaat tgcgcacgca
     1921 aggttttgag cccatgaccc tttgttcttt tttagcgcgt cttggcacat cactaccgat
     1981 tcaacctttt ctttgcctag aggacctgat tgagagtttt gatcttgaga catttagtcg
     2041 caatcaacct cgttttgatt tggaagaact taaggagctc aatcataaaa tgatggcgat
     2101 gatgcccttt gatgcgatgc atccaaaact tttggcacgt ggtttttcaa aattctcaga
     2161 accagagtgg gatttgttca aagggaatat cgagtctgag gaggatttta agtcttggta
     2221 taccattttt cacggagata tctctgtgcc taaagatctt gatagagaat acatacatac
     2281 ggcccttgag cttctctcca aagcaccgat tacccaggaa acatgggcac agtggacggg
     2341 ggttttaaaa gaaaggacag ggcgtaaggg aaaaatgctt tttatgcccc ttcgtcaagc
     2401 ccttacagga caagatcacg ggccagaaat gaaagattta ctccccttgc ttggctatga
     2461 aaaggtcaag agaagactga gtctcttata gtttactcat ccgtattaat tgattatttt
     2521 tatataaagc ataatagcta tgggaatggt cttttttata aaattcgaga aatttgattt
     2581 taatatcaaa tacaacggaa cagtgatttg aaaaaattaa aaaattaacg gatcatcttt
     2641 attcaagtta aaatagaaaa ccttttcttc taaagctgcg gggatcggaa tgaaatggcc
     2701 tgcaccaagt gtcatggctt tttgcatcaa agaatatcct tggttgtcgc cctgctgaat
     2761 caaggaagcc cctttaaacc aaagtccgat ggcatcatca gggttattgc taagaatgtg
     2821 atggcagagt gtatccaaac tctctacttt ctgatgcccc aggagaaaac gaaaataagg
     2881 aacaagcaaa tccgtgcgtt ttggcattgt atattgtgcg attgcgacta aattttccca
     2941 ctcactgaat agcttttcat ttttatcacc cgtatttttt gtatctttca cgagatcttg
     3001 cacgaggcat ttaaaaaaac aatccatgag actcaggtgg cttgtcagtc ca
//
LOCUS       DAIAHG010000142         3040 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_142_length_3040_cov_3.367533, whole genome shotgun sequence.
ACCESSION   DAIAHG010000142 DAIAHG010000000
VERSION     DAIAHG010000142.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142_length_3040_cov_3.367533"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          849 a          617 c          615 g          959 t
ORIGIN      
        1 tattatcggc tttattcaaa aatttgtgga cagagaagtc tatgatgtac acatgcctct
       61 tacgaagaaa aaaatgtttg acgcgcacgt atctgctgct cttgagcttg agcattgtga
      121 ctattttatg ccgatgaatt taagtgtggt gaactcaggc tcattttcac acaataaatt
      181 taatcccctt ttaagacata ttttatctgg aacgtccaaa gtttcttgga tattggaaaa
      241 gtatggcctt aaaattccca agaatcggtt gacctctccg tatattatga ctgttgctaa
      301 ggtttaaagc atgatcaaaa ctggcattac aaaattaaat cagctttcta atgacaagac
      361 tttttgtcgt aaagagctca aaaacgtaag cattacgctc tatgatcaaa cattacacct
      421 tgtggatggt gatcaactcg ctgaacgaat tcttttattg ttttctgatg aaagaggagc
      481 ctataaacgt acctaccaaa aacggtttga agcgtttgat ttagctgtta taaaaatgct
      541 aaaaaagctc ccaccccccc aaacgtttca tgattgtggg gtgagtgatg gtcgcacagg
      601 cttagatttt tttgaaaaaa tggtatccgc gtttccagag attcagtatt ttgcatcaga
      661 ttataaccct tctgtttttg ttttggaaaa aggaaagcta aaagtgacct taagccatac
      721 aggaaaaatt ctagaaattt tatttccacc gtttgtgttt aataaaatca aacgggatag
      781 ctttcgtcat tatcctttga accatttgat tctgtttttt attgaaaagt tttgggttaa
      841 ttctttaata aaaaagtacc agcaagggct tgttaaagca aaagaacagc tcctttttgc
      901 accaaaagtt ttgcaaaaag cacaaacaga taaaaggttt cagttggggc agcacgatct
      961 actgcaacct tttaaagaac cggattgtat cattcgagca atgaatgtct taaacccctc
     1021 ttatttttct gaaactgaat ttgctcaagt catccgcaat ctttatgaag gattgagaga
     1081 aaatggactt ttgattgcag gatctaacca agaggccggc actattgtga atggcggcat
     1141 ctatcaaaaa aaaggaaatg gtttttcgaa agttttcaac tccggtgagg gggcacccat
     1201 cgagtcactt cttcttaatt ttaaaattta aagtgttttt tatgcatctc gcccttatta
     1261 tcatatcatc tcttaactct ggcggagcgg gttttatcgg attttgccaa ttattgggtg
     1321 tcaaaaggag accaagtagc acgcattacc ttggcctcac cggatgcaaa gcctttttat
     1381 ccttttgatt ccaatgttcg tttgatccaa cttaatcaaa gttagaatga attttctttt
     1441 gtaacgcgtc taggaaatat cctcaggcgc gttttttgtt taagaagagc tctgaaaaac
     1501 cctaaggccc gatgtggtgc ttttttttgt ggatgtgatg aacctcacta cccttctggc
     1561 aagcgttgga attaaaacac ctgtgattgt gtcagaacgt acgcacccct tttaccatac
     1621 gcttctttct acttatcatc gtttgaggac cattttatat ccaaaagctt tttgagtggt
     1681 ggtgcaagcg caatcagcag ctgatttttt taaagagtcc tcgttaagga aatgcattat
     1741 actcgatcaa gccagctata ttgcctctgt gggagagact atgttcattt aaggggggtt
     1801 atagcctcac tcacctattt gtggtgcaca tccttcaacc aaccattttt tgctcttacg
     1861 atttatgtgg agtggcttga tcggggaggg gggcaaaagc tcatcaactc tttaaacatt
     1921 caaaacagga gattcatcaa gagcttgtgg atgctgattt atttgtgttc ccatcccttt
     1981 acgaaggctt tccccatacc ttagttaagg ccatggaggt aggcccaaac tgctcgggaa
     2041 atattgatat tgtcagagac gacattgatg gacgcctttt tcccgtgggg gatgttctta
     2101 aattagcctc tattatagaa gagtttgtct gtgaacaaaa cactagagag cgtcttggtg
     2161 aggaagcaaa aatcgtcgct gagtgttttc atcccgacct tatttttcac aagtgggatc
     2221 aggtcattcg tgaggtaaaa ggccagatta tctaattcct atggcaaaat ttttgcttat
     2281 ccagcagatc catataatcc tgcagcacag aaataagcgg gctttgctca ggtaatgcag
     2341 caaccgattc ttcaattttt tgccattctt gcgcagtgta atggggtgcc tccggtcaat
     2401 tacttgtctg cactttcccc agatttgcgc agccgcgcaa gcagacaaga cttaccccga
     2461 tgttgacaag atttattttg ttcatccaat ttttcatagg tttcatgaag ctctacgttt
     2521 cctctgcttg ctcacgcttc aaccgttcct ggccaagcgt ataagctaaa aaagtttggc
     2581 taaattcgcg aatagcggca atcagtgcag atagccaaag gggcatcatc gctgcaatgc
     2641 tcgcaactga agcttcgttg agattgagct ctagttctga tgcgcctgca acagtaatgc
     2701 ttccgattaa ggttgcccaa aattctgtag ttttaatgcc tgctttcatt ttttatctcc
     2761 aatgttaaag cttatgcata tgctcgtttc aaccaacctt tgaggaattt agcccgtcgt
     2821 ggttgagtgg cggcgagtgt tcgataatat ccagcggctt ctgattttaa ggccgcaagc
     2881 aagtcgctaa gatcagcctt attaatagca gcaagtgtaa tggggcctaa cacaccatcc
     2941 tcagcaatat cttgccctgt ggattttaaa aaactcggcc gcaagggacc gagtttcaaa
     3001 ataactttaa ctgcacagcc cttttctcaa ccttatccat
//
LOCUS       DAIAHG010000143         2996 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_143_length_2996_cov_3.422062, whole genome shotgun sequence.
ACCESSION   DAIAHG010000143 DAIAHG010000000
VERSION     DAIAHG010000143.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143_length_2996_cov_3.422062"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          901 a          668 c          535 g          892 t
ORIGIN      
        1 ttgattttcc agagccgttg cgtcccacaa ggcaaacttt ttcacctttg tgcagatgaa
       61 acgttatatc tttaaaaaga ggttttcctc caaaggttag tttaaggctt tgtgctgtta
      121 aataagggcg cgccatgaaa atatttttaa tgaatattga atcgtgccta ctatacctca
      181 aaagaaagat aaaaatgtat ggctaaatct ttgaaggatg gcttttcaaa gtaagtatat
      241 gtgaagtttt ttaggattgg ttgttttatg attttagtaa catttcacag agtttttttg
      301 aaaaattcct ggaaattaca caaattcctc taaaaaaccc tgaattggag gataaaaaga
      361 aaaagtaaga taaaggtaaa aaaggtagat gcatccaccc tcaccttaaa agataaaaat
      421 ttcgattaaa aatactaaaa atggacttaa ttccttttaa aacacagcaa acaggatttt
      481 tctttagcgt tgatcaagtt taaattcaat acggcggtta cgcgccatcg cggcctcgtc
      541 cttaccctct gccagcggct gatactcccc aaaacccgcg gccacaagat gcgtgggatc
      601 aatcccttct tttgttaacg ctttaacaac agcgatggct cttgcactgg aaagctccca
      661 attcgaagga aactgtgctg tattaatcct gagcctatcc gtatgcccat ctacgcgcag
      721 cacccaatta atatcttttg gaatattttg ggtaatattt ttaagtgtct tggcaagggt
      781 tgccagttgc gcctcgccat cagcgcgtat ttctgcagat ccccggtcaa aaagcacctc
      841 ggattgaaag acaaaacgat cgcccacaat gcgaatatca gagcgctccc ccaaaacttt
      901 tacaagtttt gcaaaaaatt ctgaacgata ttgggtaagt ccaagttttt tcggatcttt
      961 tagttttgtc acttcacgat caagtgtttc attgaccgtc aaggcttctg ctaatttatt
     1021 gttaagatct ttgagctcct caactttcgc aagtaactcc tcatttaatt ttttttgaag
     1081 ctgatccagt ttcaaagcct gatcattcga atgcgcctct tcctcctcaa gggcactact
     1141 caggcgctta atttggtgat ttaaatcagc aatctgggca ttgagatcac taatagaaga
     1201 aaatgctgct tctttggctt ctgtttcttt tgtgagggtt ttttctgaat cttgaagagc
     1261 atggcgcaag tgcattactt gattttccaa aaggtccaag gtttttttca aattcatcat
     1321 ctgaagatga gcttcatcct ttgagagctt ttcaatctta agatcatttt taagcgattt
     1381 aatctgcccc tctagcgcaa ggagtgcatt ttcttttccc ttaatcgcat ccgttagata
     1441 caattgcgcc acaacaaacg tcataagcac aaacacaata accatgagga gagtcgccat
     1501 ggcatcgaca aagcctggcc aaatatcaag agaaccatga cgacgagagc gtgcgaacat
     1561 accaattact ttatgagaag atttgccttc attctaatca atataggctg taaaaaatag
     1621 cctattttca acacgttttt tgaatgcacc gaggatgtat aaaaaatttc taaaacgcat
     1681 cactgagttt aaatgactca ctcgtgtctt tgagtggttt taatctgtct ctataaaaac
     1741 tgtttttaga cacgcccatt aattttttat aaacctcttc gagtcgtttc aggtcttttt
     1801 tctctgaaaa ggtaaggagg cgaacaagat tgccataatg ccgcgaatcc tttgcaaagt
     1861 ataaaatggt ttcatccttc atcgataaaa caaccatcga catggcaatc aacgcatcct
     1921 ttgtaaagca ttcaaaggga tcctcaatgc cataaacacc acctgcatcc aaaaaataaa
     1981 aaaacatttc cttgtcgcga tagttctcct catccctcgt tttcttgagg tccatcacca
     2041 tcacattacc aagcgttttt ttaagggaaa tattgtcttt attttcctcc tcccccagca
     2101 tcaatagagc attaaagcgt ggaatattat ttttcttatc gttatcgcaa ttattccaaa
     2161 gagataaaaa ttcatcgaca gcgtcttttt ctgaagcaat aactaaagag ttagtgagaa
     2221 aaaagagacc gatgataagg gcgaagagtt tcactgtatt attcccctac acagctacag
     2281 caatatctgg cgcatccaca gctttcatgc ccacaacatg ataacctgca tcaacatagt
     2341 gtacttcacc cgttacacct gaagaaagat ccgaaagtaa ataaactcca gctccaccga
     2401 cttcttcgat ggttacattg cgcttaagag gcgagttata ctgattccat ttaaggatat
     2461 aacgaaaatc gccaatgccc gaagcggcca gggttttaat cgggcctgct gaaatcgcat
     2521 tcacacgaat attatctccg ccaaggtcca ctgcaagata tttcacactc gcttccaacg
     2581 ctgcttttgc aacgcccata acattataat gaggcattac gcgctgtgct ccgtgatagg
     2641 tcagtgtcac aatcgaacca ccttttggca taagcttgct tgcatagcga cacacttcga
     2701 caaatgaata gcaggaaata ttcatcgcca tttcaaagcc ttttttggat gtgtcaacaa
     2761 acttccccgt cagctcattt ttatctgcat aggctaacgc atgaacaacg aaatcgaggg
     2821 tgccccattt ttcttcaatt tccttaaaaa cagccgcaat ctgatccgca tcactcacat
     2881 cacagggcat aataaagtcg gatcctacag aggctgcgag aggctgcaca cgctttaaaa
     2941 gagcttctcc catatacgta aaggcaagtt cagcaccttg ctcagcaagt tttttt
//
LOCUS       DAIAHG010000144         2967 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_144_length_2967_cov_3.211765, whole genome shotgun sequence.
ACCESSION   DAIAHG010000144 DAIAHG010000000
VERSION     DAIAHG010000144.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144_length_2967_cov_3.211765"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          854 a          642 c          635 g          836 t
ORIGIN      
        1 gcacaagctg ttccaagaat tttaaggatc atctcggtct ccacaagctt tttatcttaa
       61 taacactgtt taaaaaaaac acaattgcgg tttggtgccc tcggggagag tcgaactccc
      121 acatcctcac agataacaga ttttgagtct gccgcgtcta ccagttccgc cacaagggca
      181 acttagcggt actataacca caaaatgatg cgttggctag aacattttcg atagaatctt
      241 tgaaggaatt gttttaagga gtcttcttca ctttttctta attatttttc aattataatt
      301 tcttacatca gaacccatat tttaaaattc tacgatgacg aagttcttat tcttactcac
      361 aattcttctt ctcactcagc ctgcgctgac gagtgaaatt aatgacaaag aaaaaatttc
      421 tgagaatgaa aataaaattg aaaataaaat taatgaaatt aacgttgaga aaaaagaaat
      481 caaacctttt ttcgcgaaag atcgcctgaa gtttattcaa cacctgtttg cagaaaataa
      541 tgacctaacg atgacaacgc cccttggcga tattttgtat aaaacacttt acctcaaaga
      601 cctcgccttt attcgccaag agattgaaaa tacgacagaa tcagggtgga tggcgcgtaa
      661 taaaaaatgg gtattacgtg agcttaatga tcttgacacg tttgtcaaaa aatatatggc
      721 tgatgacatg tctttggccg attatgcctt gatcggctac ttttgggcgg agattttgag
      781 tggcggcacc cttaccgaac atgatctttt ttggaaaaaa cccctgaatg aacaatctga
      841 gtttcagcaa aagcatggtt atcctatgcg cgcgatcagt ggaaaaagta ttctaaagcc
      901 gctgtgatcc tcgctcctca tatttccaag acgggcggtg tggctgtggg gacgtgggat
      961 gatgcccttg gtttttgggt caatgaagag gaaggaaagg ccagtgctgt gtggttttta
     1021 gctgtgggca ctgattatat gcagatcttt gactatttaa gagaaaaacg ccccagggat
     1081 ggttttaaac acgatgtcga gcattgggaa cgtgctgagt atgaaaaata tcctcagcgt
     1141 cttccaaaag ttgccttttt taaaaatcac ttacgtgaaa aaataacgga tgagacggaa
     1201 aagaaactcg caagtcttgc ctttttctat ctttttcatg agcgtcaggc gtcctatgac
     1261 acgatcaaag ataaactgac agaaatgatc agtaatttta aagaagaacc caaaaggctc
     1321 tatcaaatat ccacggataa ttatttttat aaatacaatg gctatccaca agatttcgac
     1381 agcattgaaa tgcttgaggt cgatatcaaa aatgccaaaa gttgcggcat caccattgag
     1441 ggcaaagatg cgcgtgaaca gaaaaagaat tttgtggata ccctttatgc ggggtatgcg
     1501 cttttattaa aatacgccga tgcctatgat aattatacac cgctggagga aaaacgctca
     1561 aacaacaaaa tagagtaaca gacgaacttt atttctgaga aagcctgtga tatcgtgtca
     1621 cagcttttct tttttgattg tgatctatgc gccttgacct tgtgacccaa acaaaacctg
     1681 attatcgctt gctcagcaat agcttgcgtg ttttatcgat ggatatggtg gagcatgcaa
     1741 attcagggca tccagggctt cccatgggta tggctgatgt cgccactatt ttatttcaag
     1801 attatttaaa atttgatccg agccaccctg attggttgga tcgtgatcgc tttgttcttt
     1861 ccgcaggaca cggctctgcc cttctctatt ctctccttta tatgtgtggt taccctaaaa
     1921 tggccattga tgagatcaag cgctttcgcc aacttggaag cctcacaccc ggtcaccctg
     1981 aatacggtca cacgcccggc gttgaaacga ccacgggccc cctgggtcaa ggctttgcca
     2041 atgctgtggg catggcctta gcggaacgga tgttaaacgc acgctatggg gatgatctta
     2101 ttaatcatta tacgtatgtt attgcgagtg acggtgatct tatggagggt atttcccacg
     2161 aggcactgtc ttttgcgggg cattatcaac tgagcaagct cattgttttt tttgacgata
     2221 ataatattag tatcgatggc aaaacggatc tcacagtttc tgataatcag cttcaacgtt
     2281 ttagtgcctc ccattggcat gtccaggcga ttgatggcca cgactttgac gccattcata
     2341 gtgcgatcca agcagcacag caatcagaca ggccgagcct tatcgcgtgt cgcacacata
     2401 tcgctcaagg atcgccgaac aaagccaata catcgcaggc ccatgggtct cccctcggcg
     2461 cagatgaggt gaaagccaca cggcataacc taggatggaa atcgcctgaa tttgtgattc
     2521 ctgaggatat tttacttgat tggcgccttc ccggtcaaag gggccataag cgctatgagt
     2581 cctggcgcaa agaatttaat aaacttgatg aaagcactca aaaaaggctt tttgccaccc
     2641 ctaaggattt tgcaaaaata attgcagccc cctttcaaca actcaaagaa cagtttttaa
     2701 aagataaacc gtgcctcgcc acccgtgttc tttcccaaaa aaccctcgat gttgccgccg
     2761 atgttttacc tgaacttgtc ggcggaagtg cagacctctc tccgtcgaat aatacaaaag
     2821 cacgctctca aaagcctcta acaccctttg attttagtgg ccactatatt cattatggtg
     2881 tgcgcgagca tgctatggcg gccatgatga acggtatgag cgtgcacaaa ggccttatcc
     2941 cttacggtgg gacattcctt gttttta
//
LOCUS       DAIAHG010000145         2962 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_145_length_2962_cov_3.178856, whole genome shotgun sequence.
ACCESSION   DAIAHG010000145 DAIAHG010000000
VERSION     DAIAHG010000145.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145_length_2962_cov_3.178856"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          911 a          625 c          612 g          814 t
ORIGIN      
        1 tgcagcaaaa taacagccaa agtccctgca tacgttccaa taaaaaaagc ggtccccatg
       61 cctaaaaaaa tcatcgacag cacaagcgct gtgggcctta ccagtaggct gagagatttg
      121 agctgccata aacgaatact gtatccaagg ataaaaagaa caaagctata ggcataaaat
      181 ccaaaaattt gaaaaagaaa atctgctgta tatgatccta tgactccaag agggtgactt
      241 acagccatgg ttgttgcggt attaaaagaa ggatcaaggg gcgagtaata atataaaacc
      301 gcaaacataa gggctgctag ccccacacat aaaagactga tcatcctttc gaaaaagtgt
      361 gtaaatgtct caagaagacc ttcagggaga agcatttttt tcataaggac ttaaacgaat
      421 ttaaagagat tttttgagca ggttacgtta acgtatatat gtcaacctat agcagatttt
      481 aaagaaaaga caaagaaatg gacagatcat gacccaaaaa ccaattttat cctatgactg
      541 cgttattctt ggagcgggcc ttgttggcac tgtcatggcg ctacgccttc atcatgcttt
      601 taaggggaaa aattttaaaa tagctgtgat cgagccacaa aatattaaag acgcatcctt
      661 ttcttttgac ggaagaacca cagctatttc ctatggctca tcacttattc tgaaaaaaac
      721 agatctttgg gaagaaatta gcccccatgt caatcccatt caaaaaattc gtgttcttga
      781 atctcatact ccttttactc ttaattttag ccaacaagaa ctccatcacc ttgatcatcc
      841 tatgggctat atggttgaca acagtttgct gcgcagtctt tttcaaaaaa aacttttaga
      901 gtcggctatt gcccttaaac ttggtatcac agcacagtct tttgagccaa cctccaaagg
      961 tgtagtcata acactttctg atcaaacaca gatccgtgca aaacttatca tcggtgccga
     1021 aggtaaacat tcacccagtc gtaatcattt tggcatcaaa gccattgcaa aagattatgg
     1081 gcaaacagct atggtaggca ccgtggctca tcaagcgcct caccaggata cagcttatga
     1141 agtttttcac ccaaaggggc cccttgcttt tttgcccatg gcaggtgata caacccatcc
     1201 ccataaaagt gcgcttgtgt ggagttcttc tgaaaatctt aggtttatgg atgaggcaac
     1261 ccttgcagaa aaaattaaca acattttccc aacacttggc cctgtcacat ttatatcaaa
     1321 aataatgcac tatccgctca gctatcaaaa aacgtcatcc atggtaggag accgctatgc
     1381 tcttattggc gatgccgccc atacaatgca tcctgtagca gggcagggtg tgaatctggg
     1441 gtggcgcgat gcagatgttt tagccgatgg tatcataaaa acccacgctc ttggccttga
     1501 tattggtgca caaacgcatc tccaggctta taacaacaac cgtcaaaagg accaaaaatc
     1561 ctttttgtat acgacacaca gtctcataaa actttttagt attgagtcaa caacccttca
     1621 aatgctacgc tcctacggtt taggactcgt gaatcgcatc cctcccctta aacgtttttt
     1681 gatgaaaaaa gccatgggga tttaaggcag gtagataagc aagtgccata aattttattt
     1741 gcataacctc ataattggga attggtcaat ttttgatttg aaaagaatgt cactgatctc
     1801 cataggagga gatcattata aaaaacacaa atacgtatca accccttcat tttaaaaaaa
     1861 tcttgatttt agaagctatg tgaggtatac aatggcctta agtgatttta tagattagag
     1921 aaattaacgc catggctgtt ccaaagaaaa aaacatctca atcccgcaaa ggcatgcgta
     1981 gagctcatca tgcgctcaaa actgtggaaa gtgttgaatg ttccaactgt ggatcgcgca
     2041 aacttcccca tcacatttgt gattcttgtg gtcactataa agggcgccaa gttttttcac
     2101 aaaatcaagc cgaagttgtt gctgactaat tttttaggtt gccctccatg accactgtca
     2161 ttgcgcttga tggaatgggt ggtgatggtg cgccggcgat tgttttagag ggtgctgagc
     2221 gtatcctctc aggcaaaaaa gatattcatt ttcttatttt tggtgatgaa aaaacgctga
     2281 agagtgcggt agagccccat ccccttctga aaaaaaatgc tgaaatttgt cacgcaggtc
     2341 aggctgtaac aaactttaca aaaccttctg tagccctaag gcaagctgca gcatcaggta
     2401 tggggcttgc gataaaatct gttgcagacg gtcgcgcctc agctgttgtt tccgcaggta
     2461 acacaggcgc ttatatggcg ctttccaaga tgtttttaaa gacgattgag ggtgttcaca
     2521 ggcccgcgat tgctaaaatt atgcccacag cccttgggcg aagtgttgtc cttgatctcg
     2581 gcgcaaatgc agagtgcgac gtgacaaatc ttgtgcaatt tggggtgatg ggttccgttc
     2641 tcgctgagta ttcggggatt tacaaacaaa atcagtcccc gcttgtgggg cttttaaata
     2701 ttgggtcaga ggcgtcaaag gggcttattc agctccagca aacagccgaa attcttgaaa
     2761 aacatgtgaa tttttatggt tttgtcgaag gaaatgatat cacaaaaggc acaacggatg
     2821 tcgttgttac cgatggtttt tcaggtaata ttgcgctcaa agccattgag ggaactgcac
     2881 accttataaa actttttctc aaagagtctc tttccaaata cacaagtggc aaaattggtt
     2941 atatgattgc gagaaaatcc tt
//
LOCUS       DAIAHG010000146         2923 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_146_length_2923_cov_3.491567, whole genome shotgun sequence.
ACCESSION   DAIAHG010000146 DAIAHG010000000
VERSION     DAIAHG010000146.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146_length_2923_cov_3.491567"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          792 a          543 c          624 g          964 t
ORIGIN      
        1 acaaccgcag aagggaaaac gtctgctgtt cactttttgc attttcctat gacggaaaac
       61 caagccgcac tctttttaac aacgcaaaat gaggtgctgc tcgcaataga acatccacgc
      121 tatcactatc agacctctat tcccaaagaa acgcttgagt ctttgcgcgg tgatttataa
      181 aaattgatag tattttgtgg atgttgcaaa tatatatata gaaaaatagt ctttgtttaa
      241 aaggaaaata aatgaaacga aattttatca ttggcatgtt tttagcgccg cttcttattc
      301 ttctttactg ggggattaaa acgcttttta cacctggtgt aagtgtgagc gaggtggaag
      361 aaaaagatat tttggccgaa ctcaattata ccacagagta tgccggcggg attttacctg
      421 gcttttcttt ctcaatggga gagaatggaa ccgttaaaga ggcaaagctt gcagatttta
      481 aaggcaaacc tacgatcatc catatttggg caacatggtg tggaccctgc ttaaaagagc
      541 ttcctgagtt tagtgatttt gttaaaaaat ataaacataa atacaatatc attgctgtga
      601 gtgccgatgt ctcggattct ttggcggaga cccaaaaaat agttgatgat ctttggcgtg
      661 aaaaaaatta caacagtgtg cgtatggtgt atgactttga ggcacaactc tcaagaggat
      721 ttggcattgc gggtgttcca acaacgatct ttttgaatgc agatggaaaa gagattggtc
      781 gtattaatgg tgcgatgtat tggatggatc ctaaaattat gatcattgtg gagcgtcttt
      841 taaaatctta ggcgaccgtt tttaaaggaa tagtgggtag gaaagaggat acacttttta
      901 ttaacctttt tccgtttaga atgcttttag catgccctta ctaaagatct caatgtgtca
      961 aatgatccac ctgtctcgaa ggagaaattt tctactcttt ttataggatc tcttggcgtt
     1021 gtgtttgggg atattggaac aagtcccctt tacgcgctta aaagttgttt tacaatttca
     1081 gggttgccgg ttacagaact taatgtttta ggcatcatta gcctttttgt ctggacgctg
     1141 atttgtattg tatggataaa atattccttt cttgtcatgc gtgtggataa tcacggcgaa
     1201 ggagggatcc tcgcgctgac aacccttgcg caaaaactca aaattttttc gaataaaagc
     1261 attggcatta tcgcaagcat cttaggaaca gccctttttc ttggggatgg cattattacg
     1321 ccggctattt ctgttctaag tgccattgaa ggacttgagt gtattacgtc cagtttcaaa
     1381 ccctatacaa ttccactttc tgtgatgatt cttacacttc tttttgggat tcaaaaaaga
     1441 ggtagtggct ctattggtcg tttttttggt ccgattatgc ttgtgtggtt tctttttata
     1501 tcagtcacag gtgtggctgg tattgtgaaa aatcctatta ttttacacgc cctcaatccc
     1561 tatgcagcaa tccattttat gtatcataat acatttttag ggtttatgac cctcggcggt
     1621 gtggttcttg tcgtaacagg tgcagaggct ctttatgcgg atatggggca ttttggacgc
     1681 tttcccattc aattatcatg gggagccatt gtttttgtgg cactaactct taattatttg
     1741 gggcaggggg cgagcctctt agggaatagt gccgctcttg acaatccttt ctttgcactt
     1801 gtgcctaaag ggcttttaat cccctctgtg attcttgcca cagtcgcttc tgtgattgcg
     1861 tctcaggcga ttatttcagg ggttttttca attgtctccc aagcgatttt actcaattac
     1921 cttccgcgca tgcacatagc tcatacatct aactatcaaa ttgggcaaat ttacatagga
     1981 agtattaatt ttttattgtt tgttctaacc gtgcttgccg ttgctctttt tggctcctca
     2041 gataatcttg cgcaggccta tgggcttagt gtctcaggcg ttatgctttt aacgagcttt
     2101 ctcatcacag cagtggctta caaacaatgg caatggccgt acgggaagct ttttatgatt
     2161 tttttgccga tgatcctttt agattttatt tttgtggcaa cgaacttgat gaagcttatt
     2221 gagggggcgt ggtatactct tcttgttaca gggattattt attctattat tcgcgtatgg
     2281 attaaaggaa ataaggctct tgagtctcaa aaatctgcac cggatattac ccttgcagat
     2341 tatcttagtg cgtataaaaa acaatacaaa gtacgcattc caggggatgc tatttttttg
     2401 agtcgctttt caaatcgtgt gccgaactct cttttgattc acctccatca taataagttt
     2461 ctgcacaaaa aagttttttt tacgacgttt actattgagg aaagaccatt tgtgccgcca
     2521 aaggaacgct ttgatattca aaaaataaac aaagacggtt tttctattga ggcaaagttc
     2581 ggttttaaag aatcccctaa tctcaaccgg attattcggt tgctcaaaga ggaagggatg
     2641 attgaaaact gtgatgaggt ctctattttc cttagcaaag gcatccctgt atcaaagcga
     2701 tcgagcaaac ttaaaggatt ttcagggcgc atttacattg tcttagcctc gatcgctcaa
     2761 aacgcaagtg atttttataa aatccccaac gacaaggtta ttgaacttgg tgtgcggtat
     2821 aaagtgtaga aaagcactct aaaataaacg tacatacagg atcactcgag agggggaaat
     2881 taaacaaacc ctaggcggcc ttgagaaact cttcgacgcg att
//
LOCUS       DAIAHG010000147         2864 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_147_length_2864_cov_2.724435, whole genome shotgun sequence.
ACCESSION   DAIAHG010000147 DAIAHG010000000
VERSION     DAIAHG010000147.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147_length_2864_cov_2.724435"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          856 a          601 c          609 g          798 t
ORIGIN      
        1 tcggggtgtg gatcaagatg aggtgatggc gacgaggctt tacgagattg ctgcatcaca
       61 ggggcatggg ccgtcggaat ttaatttggg gattatctat aaaagaggtg gaggtgctct
      121 taaagcgcat ccacaaaagg cctattattg gctttaccgt gctgtgctca acgaatctct
      181 tgatgagctg cggtatgatg cagcgcttta tcgcaatcaa gtgggagagc ttctcagtca
      241 gggtgagcgc atggctatcc ttgaaaaaat ctataaggaa aaagagcaat catcctttta
      301 gaccatgatg aaaatgtgct ttttgtgtaa cagaaaaaag ttttagctcc ttaaaagtcc
      361 actgaaacat tgaacaacac cctgctttca ctataaaaag gaagagtata aagaaccctt
      421 aaagtatctt ctttaagaca attttaaaat aggagttaat tttatgaaaa aaattctaac
      481 agctttattg ctcacagcag cagcatcaac aacagcaagt gcaaacatga caggtctcta
      541 tgtcggtgtt gcaggtatca caggcgcaac aacagcgaaa tacactgatg ttccaggtgt
      601 agccaatagc gctgctggtc caacattccg catggattca ggaaaaacat ctttcggtgg
      661 tcgccttgaa gtgggttatg gtatgacttt ctctggttgt ggttggttcg gtgtaagcat
      721 ttacgcaacc gctctcaata caaagatggt tttgaatgat agccttggaa atcctggttc
      781 agttactgtt cctgctactg cgtctatggc atactcccgt gcgacattga aaaataaatt
      841 caattacggt gcagagttca aactcggtta ccactttaca aaagatacag ttggtttcat
      901 cggtatcgca gctgaagctg gaaaatacag acttgactgg acacaaaaca cttttaacac
      961 aaaagctagt gtaacgcttc gtccgactct tcgcgcaagc aagacaaaag tctatgcaaa
     1021 acccgtcatc ggtgtgcgca caacaggtct tggtggaaac cgtaacctct tcttagaagc
     1081 aaaatatggt tatggctttg cgaacaaagt tacgctcagc gttcctgtgg atgcacttgg
     1141 tgcaacaact gcagcatcaa cacagggtcg tacagtttcc gcacgcccac gcacacacga
     1201 attctccgtc acaatcggtt ggagattcta atcgccttaa aagcgatatc tttaaaaacc
     1261 ctgggagttt ctcagggttt ttttatgcca taaaacattt taaatccaaa aaataacttg
     1321 cgtttaaagt gcatcaaaaa gttcttgaat ctttgccatg ggatacggtg ttaatttact
     1381 ttcctttcga agcttgtaga aaaaatcatc catcatcagc gcaatgggca tatatttttt
     1441 gattaaaggt atgattcctg ggggaagacg atctccaaga tcgagggcaa taatctcaca
     1501 taaatcaatc ggccattttt cttttccaaa aagacttgta acgatacaat gcaaaaggtt
     1561 caccacaaaa cacgcctcgt cataattgtg atgataattt aaaaaatctt tttcttcttt
     1621 tgaaagatta aagcttgagg aaagtgctaa accaaaatca gaaaaataaa gatgctttcc
     1681 atcggttaaa agattatgaa aatgtgcatc aaagtgccta aaatccttgg tcttcataaa
     1741 tgcacctgtg gcttttagtt ctctttctgt ccaataaatg gctttttctg cagcactacc
     1801 tcctttttga atggctacat tgagccagct aaaaagcgtt tgcggaacat attcaaggaa
     1861 aagggtcacg cacatcgttg cattatccaa agactcaagg cgatgacgaa ttgcttttga
     1921 attttcccag tagcgacaga tgtcatcact ctttttttcc tcatcatgat tgtttcttgg
     1981 caaaatgcgc caatgataga gaagcggaaa attaaggcat tcccccgcta tcaccgagtt
     2041 cgttgtgaga atgtgtgcag aaagctcgcg ccagacacca aaacccgttg atcccacacc
     2101 atattgatag aaaagaggca actcaaaaag gtttgccgtt gacatcaaat gttgaggcga
     2161 ttgctcaaga tccgtgaggg gaattttttt tacaaaaatg gggatatctt cgataaaaat
     2221 ggacgaggct gtccccccaa tacttttaga tagcggtttt gcagcagcca gaagtttttc
     2281 aagctcgccg tccgttaagc gtgcaagggt gtttgagacc ttttcataaa tttggatacg
     2341 aggaggagtt tggtgcatga tacgactcct ccgtgaacac tatgactaga cattaaatct
     2401 aaaatggaaa acatcaccat ctttggcaac ataatcgcgt ccttcaaggc gtaattttcc
     2461 agcagcttta gcaccggctt cgcccttgca cgcaatataa tcgtcaaaag caatggtctc
     2521 tgcgcaaata aaaccgcgct cgaaatcagt atgaatgcat ccggcgcact ggggggcttt
     2581 ggatcctttc gtggttgtcc acgctcgtgc ttctttgggg ccaatggtga aaaatgtcaa
     2641 aagatccaaa agtttatatc cttcggccac aatttgatta agccccgttt ccttgaggcc
     2701 caaactttgc aaaaattcta attgatcctc aggcggtaat gttgatacct ccgcttcaat
     2761 ggcggctgaa atcaatgcga gttcgaatat gagcgcagaa gcagttggaa ttcaacttta
     2821 cgtccagtcg aaaacaagca attgcttttc tattatcggc agtt
//
LOCUS       DAIAHG010000148         2851 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_148_length_2851_cov_2.880678, whole genome shotgun sequence.
ACCESSION   DAIAHG010000148 DAIAHG010000000
VERSION     DAIAHG010000148.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148_length_2851_cov_2.880678"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          812 a          591 c          660 g          788 t
ORIGIN      
        1 ttattgagca cataatttta tagagaaaag ggcacaatcc ctccaccaat agggggcact
       61 gtgcctcctc aagagcatca atcataggtt gaattttttg tggtccttcc atcatcatag
      121 tcaagagcaa ctttccaagg tgttgcgcgc tatccgaaga gacctctggt tttgatgtgc
      181 gaggaaaaag aatctttgtg cagcgcgata aaagggttgg aagaatcttt ccaggctggg
      241 ccgaaagcag aaaaataacc gttttttggg ggcattcttc aagtattttc aaaagagcat
      301 ttgtcgattg acggttaagc gactctgctt ttgcaatcgt cacaacgcgc catgttggaa
      361 gtgttggtgt tgttttaaga aaatttaaca gtgtatgcgt ggcctcaagt gtgatttctt
      421 ttttatccac caaggccttg agcgcaaaaa aattaggatg cgaggtatac ttttcaccca
      481 ggagaaaaat tgcaaaatca tcaacggatt cgtctaaatc ttcgccaatc cataattgca
      541 cagggggaag ctgtccttga tcgacaaggg atttaagcga agaaaggggg tgattgtggg
      601 gcatacttta aagcgtcaaa gaataatcag ggaattatag caaaaaatca ggtggaggta
      661 agacacattt gttttatcac acgcaagaca tgactgcaca ttcgtatcaa aataggttat
      721 aacggttaca aaataatgtg cagcgaataa agttaaaagg tgcagcgatg gtcaaagaat
      781 ttttatgcga gatctatagt gaggagatac cggcgcgatt tcaacaggca gcagcggctc
      841 agctgaagga attattcacg caacagtgcc gtgagattgc gcttgctgca caggatattc
      901 atactttttc aacgcctcaa cgcattatta tccacgccca tatagccgag gaaattcctg
      961 cagaaaaaat tgaaaagcgc ggacctcgcc ttggtgcacc tgaaaaagct atcgagggat
     1021 tttgcaaaag tattgctgtg acaccagatg aactcactga aaaggatggc tattacacag
     1081 cagaaattat ggttccagct caaaagacag aagcggtgct ggcagggctt ttgaatgctg
     1141 tgattttaaa aatgcaatgg gcaaaaacca tgcgttggcc tcaaagccca acaccgtggg
     1201 tgcgtcctgt gcgtcatatt tttacacttt ttcacgggca accctttgaa acgtttatcg
     1261 acgcttttaa tcttaagacc cattctttta cctttggaca ccgctttctc agcccagaac
     1321 ctctttatcc taaatctttt gaagaataca caaataaact tttggacgcc catgtcgtga
     1381 ttgaccgtga tgcacgaaag cgtcttattc atgaggcttt ggaaagtgag gctcaaaagc
     1441 ataatttaac ccttattgca gatgatcgac tccttgagga aacagctggc ttagttgaat
     1501 atcctggtgt ttttatgggg aagttagatg cgcatctttt caaagcgccc gaggaagtga
     1561 ttacaacatc catgcggttt caccaaaaat gctttgcctt tcgtgggaat gatggcgcac
     1621 ttgcaccttt ttttgcagcc attattaata cgattccaaa agatcatgga gctgcgcttg
     1681 caaaaggata cgagatggga atgctcaaag cacgccttga ggatgcacgg tttttttacg
     1741 aacaggatcg acgcacaccc cttgaaagct atcagccaaa gctcgacggt attatttttc
     1801 atgaaaagtt ggggacgctt ggggataaaa tcaggcgcat gcattttgcg tcaaaagact
     1861 atgctgcggc ccttggtctt gaaaaacaag ctcttctcac acgcgcgatt gatctctgta
     1921 agtcggatct tttgacgcaa attgtgggtg agtttccaga gcttcaaggc gtgatgggaa
     1981 aaatttatgc acagttacaa ggtgaagatg aagtcgtagc agatgcactt tgtgattatt
     2041 atcatcccac aagtgctgag gcgagtggag aggatgttcc tcataaacag gtaagtatcg
     2101 gcttagcgct tctggataaa cttgatacgc ttgtgggctt tattggtcac gggattcttc
     2161 ccacaggatc aaaggatcct tatgctctcc gccggactgc ccttggggtg atgcgcattc
     2221 ttttggagaa taaaataatg atcacggtgg atacactgat taaaatcaca ctgacatcct
     2281 atacgcacct cagtccatcg gcagaaaaaa taaccaagga tgtccttgcc tttcttgcaa
     2341 cacgggcgac tttttatttc aaggatcaag gctttagtca cggcgttatc aaggcctgta
     2401 tgcccgaaga tgacacaaat cttaatatca ctgccgcttt tttgaaaatg acagccctac
     2461 atcaattact tggcacggac acaggcgctg ctcttcttgc ggggtataag cgtgcggcga
     2521 atatttttcg tgcagaatct tcaaaagata cctctgagga ttacacgctc atgccggatc
     2581 tttttcaaaa agatgtcgag aaaaatcttt ttgaggctct cgacaatata acatcaaaat
     2641 ggcacgacac tgtttcagat cacgaaaagt gtgcactttt agcctctctc aaagagccta
     2701 ttgatgcttt ttttaacgaa gtgatggtga atgatagtga tcctgtgatg cgctcgaatc
     2761 gtttggcgct actccacagg attatccgtt gttttgagtg ttttgcgcgc ttccaagaac
     2821 tcgattagac gcgttgtcat atcctcaagg g
//
LOCUS       DAIAHG010000149         2831 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_149_length_2831_cov_2.422658, whole genome shotgun sequence.
ACCESSION   DAIAHG010000149 DAIAHG010000000
VERSION     DAIAHG010000149.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149_length_2831_cov_2.422658"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          786 a          582 c          515 g          948 t
ORIGIN      
        1 cgggttgcga tttatttgcc catgatccct gaaattgtca ttgctatgtt agcatgcgca
       61 cggattggtg cggtgcattc ggttattttt ggtggttttt ctgcacaagc aatctgcgat
      121 cgcattcaag atgcagatgt tgtcctcgat cttatgatcc acgatcttgg gtgtgtcctc
      181 gatcttttgg gtgtgaatgt gcgtttttta gaaattttat cggctcatgg aagtggcctc
      241 aaaccatcga tggtcgatac agccgtggct gtgctgcgta caaaaaactg tatgattacg
      301 cttcaagcca atcgcaaaag ccatttacgc caccgcgagt ttattttgca cacacaaaat
      361 tttagttatt ttctcaattt tatctcgcag caagtttctg tgtaccgcaa taatcaattg
      421 atgtctcaaa caaatcatcc ctggctcccc ccccttgagc atgaaattag tcattttatt
      481 gagtgtgcgc gcaatcctga aaagcagcca ctgacatcgg gaatgaaggc ctctgtcaca
      541 atgaaatatg ttgaggccat tcaaaagcgt atctatcagt cctaaaaaat tgtcgcagtt
      601 tttattcgta aacaacgcgc ggatccacca tattttttaa tggttttagc cgtgtaaagt
      661 gtgaaccacg ccctgactgc gaaaggtgaa gatagatata gtctgcattt tcagcatcga
      721 caatatattg gcggcactct tgaggaattt ctaacacatt attatcaagt ttttcttcgc
      781 ggggatcagt aaaaacaatg acatttttat ccaacatttg tgcaaaaagc cctgctatat
      841 tgctgtcact tggggcattt tggtatttaa gattttcgag ttcaaactgc acaatatttt
      901 ttaacgtact tgtatctttc gcaccctttt cactcataaa gtccatagca gattccttga
      961 tgaggttctc atcgctgaca ataccctcat taatcatttg atcgagttgt tgaaaccctt
     1021 tgctttcttt attcaatttt ttgcgtaatt tttggaattt atcgtaagct tctctaaggt
     1081 tattatattc atttattgga taatcatttt ctttgggctc cttgactttc aaacgatcga
     1141 tgagcatatt ggcgacaatt tctttggctt tttgaagctt ttcacccttc agttcatcaa
     1201 caagatcaag aatatagctc ttgctgcatt tttgatttgc gtatttttgt gtcattgaat
     1261 ggaaataaca caaaccatca ccttctgttt cttctctttt gaactcaata tcattggcaa
     1321 tatcacggta atagcccgag ttttcctttg ggttttgttt tttcttttta tatttttcaa
     1381 aaggaaattt tatatcccta taaagcgatg aaaatccctt gagcatggcg ttgagagtat
     1441 cttcatcatc gtgattatat ttttttaaaa caattttttt aatatcctcg gttgattgaa
     1501 attcaaaatt ttttaatcct ttgtcaccaa gtgtgtcaaa aagggctatc gtccaaaaaa
     1561 catagctcga ctgaggagaa tttaaaagat ctttggagtc aatctcacca agagcacttt
     1621 taataaaaaa cttaagatct tgatccgcta aatgcatagc ttttgtcgtt aaaaaaagcc
     1681 ctttaaaaac ggctgtcatc tcatcatcgt caggcgatct ttccttattc agtttaatcg
     1741 gtttgccgga aatcatatgg ccaatcatat acttgccaag cttttctgca cgtgggacat
     1801 tgccttcctc aagcgcagat tggagatttt cattcagata cgtcataaaa cctataaaag
     1861 ggagcttata gcctttttct cctaatttat tttccacctg agcccgtttg tattcgtaca
     1921 caaggcttcc gtaatctttt ttcaaagggc cataatcatt agcgcatagg ttttgagcct
     1981 ctatattcgc gaaaaagatc caaagaatta aaaattttat aaaatgcata ttttttattt
     2041 tgattagctt tattgctttt ttcattgtaa gggataaata ttaagaatgc attaatttaa
     2101 cggcatttct ctcctgcata aattgcagca aacgcatagc ggtctatcca aaaaagacgg
     2161 tcatcacaat gttttgtctg cggcgcatca ataaacgtta tgtcatcatt agaattttct
     2221 actttaaaat cgtctttcac cgcaagaggt cgtcttttcc ctgaggaaag aaaatctccc
     2281 ccattcttgc cgtggcttat caaaacaaaa ataggttcat tataagcatc ctcattaata
     2341 aggttcttac tctcatcaag gattttaaaa ccagcttttg cctttacaga gcaataactt
     2401 tgggggcttg acgtgtcgcc ggtaaaaaga tcttttttta ccgacagtga gtcgtcctca
     2461 cgccactctc gcggcttttc agaaaacgca ggatgcgtca caaaagtaat ggcatgatta
     2521 aatccatccc gtgtgagcgc aggactcatg ccgagggttt tataaggaat aaggccagcc
     2581 ttgtcacaat acttaagatc ttctcggccg atatcctcgt ctttcgcatc tggggctgca
     2641 ggacacggca gacgtttatg ctgaattaag tgtgtggcca aatgattggc tatgctttca
     2701 aaggtcgccg ttgttttttt ctcctgctcc agatgaagct gacttgaaaa aaaagggagt
     2761 gccaccacaa gaacacaccc cataatcata aggacaaaag ccatttccaa aagactaaat
     2821 ccagaaattt t
//
LOCUS       DAIAHG010000150         2813 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_150_length_2813_cov_2.992325, whole genome shotgun sequence.
ACCESSION   DAIAHG010000150 DAIAHG010000000
VERSION     DAIAHG010000150.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150_length_2813_cov_2.992325"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          843 a          577 c          571 g          822 t
ORIGIN      
        1 ctcatgatcg ccctgttaag gtcagtagca gcgaggatgc agcgtttatt ctcagtaatg
       61 caagcagtgt tattattgtg cctggatatg gtatggctgt tgctcaagct cagcatgctt
      121 tgcgtgaaat ggtggatatt ctcaaaacca aggggataaa aatccgttac gcgattcatc
      181 ccgtagcggg acgtatgcca gggcatatga atgttttact tgccgaagca aacgttcctt
      241 atgaggatgt cttggaactt gatgagatta atagtgattt tgggacaacg gatgttgctt
      301 ttgtcattgg ggccaacgac attacaaatc ctgcagcaaa aacagataaa agctcaccca
      361 tttacggcat gcctgtattg gatgtagagc gtgcgaaaac ggttttattt atcaaacgct
      421 ctcttgcttc ggggtattcg ggcgtagata atgatctttt ttatcgtgat catacattta
      481 tggtcttagg tgatgctaaa aaagtatgtg atgcgattat caaagaaaca taaactccta
      541 tttacatcgc tttattataa attttattta ttgttcacac tatattaatc tttttctgat
      601 tgaatgagac cataacggaa tttttatctg aaaaggatga atgtaatgct ttttctcaaa
      661 tacatcgtac ctctttgcct gatactttct ttaggtcact cctttgcaag caatagtgag
      721 gatttaatag atattaatga ggaaaaaaaa tttcttatct ctaagaaaga ttcttttatc
      781 aaaaatcaaa aagacatgat caacaaggag gaaggccttg attttatcgg tgtgggtctt
      841 tttcgaggaa aagatccgtt cagtgatctc atatgtcttg gctcggcaag ccggtggtcc
      901 catgtcggag ccattgtcaa gggcacaaca agtcaaaaag aatatgttta tgaaacaact
      961 ggtgccccca gcgaaatagc agagggcatc ttacctgaag cacgcatcac acttttagat
     1021 gatgttgtta aaaactacag cggtaacgtc gccaaaagag aatttgtttt ctctgataaa
     1081 aaacctgatt caaaaacagg caaggactat gtccttaaaa atattggcaa gccttatgaa
     1141 aaggatttaa gcgctcttgt gaaggcgtta agagattcta acacaggatg cgatgtcaaa
     1201 agcacttttt gtagcgaaga ggttattctt ttgatggaat atctggatat acgcaaaaaa
     1261 gacaatgaga gtgaggaaaa cttcttgcca cgcaattttg gggatttgct ctctcaacca
     1321 ccctttgtgg gcacttatgg agggtcattt tcttcctcaa ttgatatttt aaagcaggtt
     1381 gaagatgagg atacttatat ttcatgccca tgcccttgta ttattctata gcacgatatg
     1441 aaaattttta tttatacatt gataggattt ttcatcttct ataggatgac gtctattgaa
     1501 gcccttaaag aagaagtcgt gatggaaaat acagaaaata cagccgattt tccatcgctt
     1561 tctttggctg aggcccagct caaacttcct ctcatttctt tttattttat tcggcacggg
     1621 gaaaccgatt ggaataaaga tcatcggatc atgggacaaa cggatattcc gctaaatgag
     1681 acaggacgtg cacaagccta taaagcagca ctgctcctga aaaataaagg catcaaaaaa
     1741 attattgcaa gtcccctgag gcggacaaaa gaaacggctg aaatcattca aaaagttctt
     1801 ggcgaggata tacctctttt cctggatcca ggtcttatgg agtgcaactg gggatcactc
     1861 gaaggacgcg tcaataacca tcttccctca cttcatgagt gggctcttgg aaaaacgcct
     1921 aagggggggg aaagtgtcac gcattttata acacgcatca gcacaacaat ggatcacctt
     1981 ttaacacaaa aaaatattct tattgtctcg cacgggggtg tgttttgggg gctcactgag
     2041 ttgcttgagc atcatcctga aaaaacagag aatgccattc cttattattt tagagcgcca
     2101 cccaaagaga atatgccttg gtttatctat cctcttgagt agctgaagat tttagaatct
     2161 taatctttaa agaccaagat aggctttttt aacgccttca tcctggataa gatcctttgc
     2221 atcgccacac aatgtaacct gaccattttc taaaacaaac gcctttgacg caatctcaag
     2281 agaggccacc acattttgct ccacaagcac aatcgtcatt cccatgtcat gaagccgcac
     2341 gatcatgcga aacatttctt tcaccatcaa cggagaaagc ccaagagatg gctcgtcaaa
     2401 aagaatcagc ttaggctccc ccataaggca acggccaatc gccagcatct gctgctctcc
     2461 ccccgacaga gtccctgcat tttgaaaagc gcgctctttg agttttggaa aaaggtggta
     2521 aacataatca atattggtct taatatgcgc tttagaacgc ttaagtgatc cacccacttg
     2581 aaggttttct aaaattgtca tgtttggaaa aacctgacgt ccttctgcaa catgaccaat
     2641 cccaagctct gcaatttcgt gattttcaca ccctacaata ttttttcctt caaagaaaat
     2701 ctcacccgac cggggttgat gaatccccgc aatagtgcga ataagagagg tctttcctga
     2761 tccattgccg ccaacaatcg ctgtcagtgc tcctttttca acattaatat tca
//
LOCUS       DAIAHG010000151         2812 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_151_length_2812_cov_3.956856, whole genome shotgun sequence.
ACCESSION   DAIAHG010000151 DAIAHG010000000
VERSION     DAIAHG010000151.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151_length_2812_cov_3.956856"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          924 a          523 c          637 g          728 t
ORIGIN      
        1 ccatcacaat tatttttgtg aaaaattcgc ctcaaaatcc taaaaattca cctattttcg
       61 tgcctaatgc gttaaaaatt acactatttt tcatgaaaaa acctcgaaat ttccgaaaat
      121 gtgtcgtgaa aatccatttc tcagagaaaa aaagggttat ttgcaaagaa aaaagcgtct
      181 aaaagctttt tgcatagtgc aaaattaaga aaatatgtaa aaattaaaat aaaaaaactt
      241 cgaaaaacat caataagttg ataaaaatta accttttttt aaatgttttc acaatagaat
      301 gatgctacgg gtattttgta tttaggctct taaatgagat ttcttattgc gctcattagc
      361 tttttgctca tcgtggagta cggggtagag tccgccattg ctaaggtaaa aaaagaaaaa
      421 tctgagaaaa agcaccctgg aaagaaaaaa acttctaaaa gggctatttg tcctcgtggt
      481 cttgaagatc aagacatagc aacggtgttc aaaggatact cttacacggg gtttttaaag
      541 gcctcaagtg agggcgatgt cgatctgatg aatggcttga tgcgcaagcc ttggggattt
      601 tacaagggac ttgatggcat tttctacgct gtttatgcct ttaagcctga atcttctgaa
      661 atggctccct ttgtggatgt ggataagctt tctgtgggga aaatggtgcg cattaatatc
      721 cctgagtatt gtgcgcttta tacggaagta tcgccagaag aggccggtag cagtgagcat
      781 gagcctgtgg gacttttgat ctccagagca tccgcctata aacctaaacc tcttgatcct
      841 ggtggagtaa aggttgagcg tatccttgaa aaaatcaaaa aaggcattgc agaagaggat
      901 gtcgatgcgg gcctcgacga agaaaacaag acagatgaga gtaatgatga gggggaaaat
      961 gaagacaaat taacacgttc taaagagcaa gaaaatggct ctacggttga ttcatcggag
     1021 gcgaaaattt ttgagaaact gaatgaacct gaagaggaaa acaccaaaga ggatacagct
     1081 gaagaagaag atcttcttca aaaggataat gatacgacgg atgacactga gagtgttacg
     1141 cctgataaag agacgctcga tggcgctatc attaatgata aaactgatga aaaaaaagac
     1201 aaagaggccc ccacagaggg ggaagctgga gatgaatcaa cctcagatga ggatctagga
     1261 acggaaaacg aaggtggcta agatagtcaa ttttttgcat ggctaaagtc tgacaaaaca
     1321 tcaatgaggc gcttttccgt atagtgaggg cgaagcgctt tgggttgaga gggatgataa
     1381 gcaaagcctg aaagaaaaag aaagtccaat gtgacaatca attttccctc aagggttgag
     1441 aatgtttctc tgtagatttt ttcaacatgt ttaagtgtgt tgttggtaat ttttttatca
     1501 cttgcataga gagcggcttg ccctccaaag gctttaatat ctttgagaag agcacccagg
     1561 gagtcgtaat gaatttcaac tgtctctcgg tcgacaacag gaagctgaaa acctgcacgc
     1621 attaagaggt tccccgcctg gtgaggggtg ataaaaggcg ctactacggg gcttgcaccg
     1681 caaaataaat ccatatgcgt ctgataaaaa gcgatagaaa gttcttgaag tgtctgttca
     1741 ccaaacatca caccgaggaa ataaccatcc ggtttgaggg catgattaac ctgtttaaaa
     1801 aagcctggaa ggtcattcat aaagtgggct gtaagatgac tgacgataag gtcaaggttt
     1861 tgtttttcaa gcggtagcga ctcttcgtcg gcacgtatcg atagaatatt tcggtgcgac
     1921 gccatcaaaa gattttgagc actatccatc tgataataag attctagcga agggttttgg
     1981 gacgataggt gctgataaaa aaaatcaagg gaactgccta tttcaagaac atgactaaat
     2041 tcttttttga caagagaaag tctttcaatc agacgcgagg atgtccactg aaaaagttga
     2101 tgaacgcttt taaaacaggc tttgtttcgg ttgcgctgga tttttttacg gtcaaaaatc
     2161 gccggaagag taaaaatagg gagagtatct gtcattcaac tccccccact ctatttattg
     2221 tggcaccgct ggaacagctt tgcgggtttt gcgggtgaaa aaagtataca ccgtaggcag
     2281 aacaacaaga gtaaaaatcg ttccaattgt cataccgcca acaatcaccc agccaatctg
     2341 acggcgtgtt tcaaaacctg cacctgttgc aaatgcaagg ggaagagccc ctagaaccat
     2401 cgcaaatgtc gtcattaaaa tggggcgaag acgcatcttc gaggcattaa tcaccgctgt
     2461 aataatatcc tccccactat cacggcgctt gttggcaaag tccaccatca taataccatg
     2521 cttggtaatc aagccaatca acgtcacaag accaatatta ctataaatat tcaggctgcc
     2581 gttttgaatg agtgcaaggg ttaggatggc tcctgagaga gagagcggca cactaaacat
     2641 aataataaaa ggatcgatcc aactttcgaa ttgcgccgcc ataatcagat aaataaaggc
     2701 aatcgcaagg ccaaagatca tgagcatcgt cttgctttca tttaaaaagc gttttgtatc
     2761 acctgaaaac tcatagcgca catcttcggg gagaacatct ttagcaattt cc
//
LOCUS       DAIAHG010000152         2803 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_152_length_2803_cov_2.829054, whole genome shotgun sequence.
ACCESSION   DAIAHG010000152 DAIAHG010000000
VERSION     DAIAHG010000152.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152_length_2803_cov_2.829054"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          799 a          548 c          614 g          842 t
ORIGIN      
        1 gtcgccttga aattttttta aaaattgtgt gatagatttt tgggtgatac ctttgtcgat
       61 gtgcatcacc atgatctctt ttgttttttt attgaacacg agatcctcga tacactcaaa
      121 cccaaggcct gtaaaacctg gattttttcc tgaaatcaac acttgttttt gacaggtata
      181 cagtgtgtcc ttaatgtcca aatccgtctc ttcaggcagt ggtttgaggg tgtatttttc
      241 gagtatttta ttgatcatag aggatgaatt tttataaact ctatccgaat aaataagtgt
      301 ttgaaccata ccatttttat aaacaactgt gactttcgct tcggtaaatt ttttattatt
      361 cataaaaagg gaatagccat gattccattc atcattctca agaatcagcg ttccaccaga
      421 tttatttttc tcgtcgatgt agtgtaatcg tagtttttga tcaggatttt tcttgtaata
      481 tgtatattgc aagcgatttt tcagttgttc ttctgtaagg ttttttaatt ttttatccga
      541 tcccaaagtt tcttggtaaa agacttgaaa gacgagaagt aaaaatacat gaatcatttt
      601 catttttttt attttttaca aaatattttt tagcgatatt ctatcaaaac ttacatcgca
      661 tgtataggcg tacatttcaa cgcctttact ctttgcatca ttcgcaagcg atgcgtaagc
      721 aggatcgata aaatctgcaa aagcaaactt cttcacatca gggcgctgca caatataaag
      781 catgacacag cggttgcctt gatggacaag ggcgctgagc tcgcttatat gtttttgacc
      841 acgttccgtt tttgagtcag ggaaataagc cgtttcaccc tttttataat gcacattttt
      901 cacttctata taacacgtgg gttggtgggg ggaggtaagc gcaaaatcaa gccgggagtg
      961 ggtgctgacc ttaacctctt tatgaatatt ttcatagcct tgaagcctat caaaataatt
     1021 atttttaaga gcctcaaaca ctattttgtt gggattgtgg gtgttgactc cgaccatcac
     1081 accatcatcc tcgatcattt gaagtgtgta cgcaagggtg cgtttaggat catcatgatg
     1141 ggacaagccc accacacgtc cttcttcgat cacacccagc atagatcctg tattagggca
     1201 atgtgccgta acgtgacgac catcctccag ttcaatatcc acaaaaaagc gcttatagcg
     1261 ttttaaaagg tagcctttga agagaggggg agaaaaatgc ataaaatgta aaaatatggg
     1321 ggattttaat gcttttttaa tctttctgtg atgaactggc aataggtata aaaatgcggt
     1381 gctttgattt atgagtgtat caaatattaa ggttctcatt gttgatgact ataagactat
     1441 gctgaaaatc atcggcaatt tgttgcgtca gctgggattt acacagattg aagaagccac
     1501 agatgggacc atggctcttg aaaaactcaa gaatgctaaa tttgatttag tcatttcaga
     1561 ttggaatatg gagcccatga gtggtcttga tcttttgaag cacgtgcgca gtgatattca
     1621 gctcaaagag cttcctttta ttatggtcac ggccgagagc aaggttgaga acgttgtaat
     1681 tgcgaaaaag gcgggtgtga gcaactatat tgtcaaacct tttaacgcgc aaaccctcaa
     1741 agggaagcta acgactgttt tgggtgaatt ttaaaatttt ttagagcagc gcaaggatat
     1801 cataatgata caacaagatc acagtacgct taaagagtgg acgcttgaat tgaaaacaat
     1861 cgatagtgct ttggaaacag ctgcaaatcg gatccttgag aattgtgaac tccttgtgga
     1921 gaaggcaggg gcctcttcgg aggttcaaga gattgtgagt gcaattatgg aaaactgcag
     1981 ctttcatgat ctcaatagcc agcgtttacg caaaattatc aaagagcttg gccttttaat
     2041 gaacgaggag ggggaaggat tgacagagag cgagcccgcc cttgcgtcag ggccccaaag
     2101 acctggtgtg gcactgacac aaaaggatgt ggagacttta ttaaaaaatt aaagggcaat
     2161 ctcaaaaagc ccttcgactt ccacgcacac accgagggga agggatgcaa cgcccaccgc
     2221 cgcacgggca tggcggcctg catctccaag aatagccacc atcaaatcgg aggcgccgtt
     2281 aatcacttta gggtggtctt taaaatcagg ggttgcggca acaaaaccac caaggcgtac
     2341 gcacatctta aggcggctcc aatccccact gagcgcttga tgaacttgcg cgaggatatt
     2401 cacagcacac aagcgcgcgg ctgcataccc tgtatcaata tccacattat caccaaggac
     2461 tccactgtag tgcaccacac cttctttgac gggaagctgc cccgaaacga tgagtgtgct
     2521 gcctgtgatg tgatagccta catagttcgc aacagccttt gcaggcgtgg gcaggataat
     2581 attatgtgct tttaaattgt ttaaaacggt gtgtgtgttc agcatgcttt aaaatccttt
     2641 cgagcgctat gaataagagt cagccatgtt tttttaaaat cttcttcgga tacagtggtt
     2701 tttttcagag attctgctgt gaattcaatg ccgataaagc gaagggtggg atcggcatcg
     2761 gtaagggctt ttgccatttc atccacataa gctttgctat cat
//
LOCUS       DAIAHG010000153         2772 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_153_length_2772_cov_3.283859, whole genome shotgun sequence.
ACCESSION   DAIAHG010000153 DAIAHG010000000
VERSION     DAIAHG010000153.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153_length_2772_cov_3.283859"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          785 a          492 c          641 g          854 t
ORIGIN      
        1 cgtggggggg gggctttttg caataataaa cgtttgcgtg tctcagggcg aaaagaactc
       61 gatacagctg tggtgggaac gggaattcct tgtgcagcct ttggggatcg cgcgatgttt
      121 ttaaaacagc ttgagcgtat tatgccgcat gttgcaggtg ttaggcgttt gggggctgcg
      181 gcccttgatc ttgcgtatgt tgcagcaggg cgctttgatg tttatttcga aggagagtgt
      241 aagccctggg atcttgcggc ggggattatt cttgtcaaag aagcaggagg ctttgtcaca
      301 gattataaag ggggcagtac catgatgcag tccggtaaca ttattgcggg taatcagttt
      361 ttcaaagaat cttttttaaa gatgatttag gcgtgttttc aaaatgataa agatcataaa
      421 gccccactaa cgttgcaagg gtaaaaaacc ccatatattt gagtatttct gtctggtgct
      481 gtggataaaa gcaaacgcct gttaaggtaa ccaaaagtcc tttgatcgaa agcgaaagga
      541 gattcttttt ttgcgtagcg cgtaattgat gttctttgtt aatagcttga cgaattttcc
      601 ttgccatttg agagggttcc tcagtttcat ttatatcagc tgcctgagtc gtattagaaa
      661 agataagaaa aaagccgagg caaagagcaa tttttcgaag catagtaaaa ataatttttt
      721 aacagttaga cggctcagtc taatttttta tgcacaggcc tgcaacaaaa attaagaaaa
      781 aattaacctt taggggcgta gttttaagag agtgatgtgt aaaaaatttt atttaaattg
      841 tgctaaaaat aatttttttt ataatgactt ttttaggagg tttaggcgag gtttttaacg
      901 cattagatct ctacgcctat acggtgcctt cgacagatac agaacaagat agtctgcatt
      961 cgtttaaaaa tgactatatt aaaattgatt ttgaaacatt tttgagtgcg gcaacaatga
     1021 aaatgagccg gtctcagtgg ggagcaggat caaaagatac aaataaccct gagatcgtgc
     1081 gaagaaatcc aggttggtcg gaatttgcag ccatgccttt gttgcgtaca accgcttttt
     1141 taaaaaatgg aggtgaaatt tactcaaaat ggagtgcgat tgccgcaaca acgaggggac
     1201 agggggacgc ttcagatctt tccacaacat ccaataaacc ttttcttctt gatagtgaag
     1261 aggcctgtgt ggggtggcga tcaaaaaaac taaattccac aaaagaaagc tttttagata
     1321 tttcaatcgg caatcaaaat gtggatatag gggatagttt tcttatctct caaggaactg
     1381 ttaatgcttt taggcgtgct gcttttttta tgagtccgcg cacagcttac cgtgaaacgg
     1441 ctgttataaa aatgaataca taccctgtgc gctatacgct ttttcatctg cgaacaaata
     1501 caaatcaacg ttatttaaga gggacagatc aacctcgtac acttatgtat ggtggggata
     1561 ttagttatgt ttcaagagat agggcaaatc cttctcgtga tttctggaca ctatcgttga
     1621 tgtttctttc tatgtataag tcagatcgta catttgatgc gagttcttat tcgcgaaatc
     1681 gtgatggttt gcaaatttat aatccacgtc ttggcggttt tttctttccg tcgaatcgtg
     1741 atattcgttt ttactcaggg tatgttcacc aaaaaaataa taaagtaggg cgaaaagtaa
     1801 atgctaatgc ctattacata gagcccgggt atgtcttttc aaaactatgg gcaaagcctc
     1861 ttttgaccta ccgttataca cattacagtg gtgatgaaaa tcctggaagt gtggttaaaa
     1921 aatcttacga tcctgtgaat tctggctttg ctgtacgcga tggctatggt acgtgggaaa
     1981 caggacagat ttttacccat tatttttcga atacgaacca aaatgtgcat aatatacagc
     2041 ttaaactcac gcccacagat aatcatacgg tagggattct ctattataat attcattttg
     2101 acaaaacaca gcaggcagga agtcagtcta aaaaagcagc aaatgaattg aatgcttatt
     2161 ggatatggac accaaggccg tggctggtgc ttacagctct tgtaggggca gcggtggcaa
     2221 aagaaggtct taatcaactt ataagagctg agaatccaac ggtttcaccc aagcggattg
     2281 gaaaaatcgc atcctttggc tgtctccttg cagcttttag attttaattg attaacacag
     2341 ataaaagtca tttttcttga cttagatagc atctattgtc actatggaga cgctttgggg
     2401 cccgtagttc agttggttag aaccccccgc tcataacggg gttgtcgctg gttcgagtcc
     2461 ggccgggccc accattttaa tcgaatctgt tggctatggc atcaaactct aaaaaaccgt
     2521 ctttacgtgt gattatcggc ggtcttggtg tgggaggtgc tgagaaacat ctgctttcca
     2581 ttttgccaag gcttgttcaa aaaggctatc atgtgcgcct tctcatgctt ggttatgcaa
     2641 gggatttaga gccttttttt gaggaaaagg gcatcaaggt ttgcacgcca aagcctctag
     2701 tgtgggtgaa gcccttgcct cattttttaa gacgtttttt ttatcttatg tatagtacgt
     2761 tgcgtcttta cc
//
LOCUS       DAIAHG010000154         2738 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_154_length_2738_cov_2.941375, whole genome shotgun sequence.
ACCESSION   DAIAHG010000154 DAIAHG010000000
VERSION     DAIAHG010000154.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154_length_2738_cov_2.941375"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          703 a          552 c          521 g          962 t
ORIGIN      
        1 acatcctcgg atgttttttc ccattcttct ttaaaaatta acttttaagg ggtactatgt
       61 ctttatgaga gaatgagagg ttcatatgca tgcagagatt aatctgacag agattacact
      121 cgtggttgtg ggcgcattga tttgcggcct tgcctttgca cgccttaaaa aaccgccaat
      181 ccttggttat attttagcgg gcattctcat gggcccctct gtttttggct ttattcattc
      241 ccgtgatcag gtgagcaccc ttgcagagct tggtgttttg ttgctgctct ttgtcattgg
      301 gatggagctt aatctgcgca catttaaaaa atactgggtt gtctctgttc tctctgtgct
      361 tgggcaaatt gcgctcagcc ttcttgtcac ggcggttttt ggtcattttc ttggctggtc
      421 attgggattg acgattcttt taggttttgt gatttccctt tcctccacag ccgttgtctt
      481 taaaatgctt gaaagtattg atgaggcaaa aacggataca gggcaaatta ctgtcggtat
      541 tttgattgca caggatttag ccatcgtgcc gatgatttta attatgcgca attttgatag
      601 aaattggttt gatatgtcac tcattataaa acttgtgaca tctgtagggc ttttagtttt
      661 gctgattgcg tatttatccc gcaagcagcg tgtgcgcctt ccccttgcgc agatgattgc
      721 cggagaaaaa gagcttatgc cgctcgcgag cctgactttt tgttttggaa ccgcggcgat
      781 ctctggtctt attggtctct ctgcggctta tggtgctttt cttgcagggc ttgtccttgg
      841 taacacccac gaacgccttg tgatgcttga tacaacaaag ccgattcaaa gcattcttct
      901 tatggctttt ttcctctcga tcggtctcct tttggatctt agttttattt ggcacaattt
      961 agggacggtt cttgtgctga tgctgttgat tacacttgga aaaacggcca ttaacattgt
     1021 gacattccac gtgctaagac tcccttggtc acagtctttt ttactgagtg tcgttttagc
     1081 ccagcttggg gagtttgcgt ttttgctcac aaccgttggg tatgaagcta aaattattaa
     1141 tacctttggc gagaaactca ttattgcgct gaccgttttg tcactctgtt tcagtccttt
     1201 ttggctcagt gccgcgcgaa gacttaaaat tcttgctgac agcaaaaatc tgtcctttgg
     1261 cggtgtcctt tattggcttt atggagggca ggtatcggct gcgcaaaaaa aatattattc
     1321 ctttaaagag gaaattatga aaaaaccgcg tgccttgacc gatcagcgcg aaaaagatag
     1381 agacactccc gaaatagagg atgatgattc tatagggaca tgagagggct atccgttata
     1441 gaataataca tcccttgcaa aagaggaggt ggcagacaga ttctttctcg ttctttttca
     1501 aagaactctt cgcgtcatct ttcattattt ctttaaaaag aatattcatc tcctcccttt
     1561 ctttgtttct ttttttctca tattcttcgt cctcctcttt atttttatcg acaagttttt
     1621 cttcaacgat ttctataaat ttttcctcaa agaacttgat tttactattc gttaaaacat
     1681 ctaacgtctc ctttatcatc tcgttgcctt ttttgattgc tgcattcata tttcctttct
     1741 tgccaagcgc ctttgaaaga gctttgcgga gtgaaatttc ttgatagagc attatagtac
     1801 gacaattggt caaaatccca agaatataga ccgcgttatt aagaggcgac tcaatttctg
     1861 catttgtaag agttttttca aaactaaaat taggcttttc aatgtatttt aaaaaaatct
     1921 ctgctgctat tgtgctcatt tcaataaaag gtttttgatc ctccacgaca atgcattttt
     1981 tgtcatcgac aagaatgata ttggatatat cctctgcaat aattgtgaga tcttttttcc
     2041 aataattagg gttattagga tatttttcat aaccagccat atgatgcctg gagtagattt
     2101 taaaataacc cccgttgatt aaaagctcat aagccttttc accctcagtg ctatgcttcc
     2161 ccaaaaggtc ctttaaaaat ttctcaatca caactttgtt gcgtttttct agacctgctg
     2221 aaaaaaaatc gacattccat ccccatgaca aaagtgtcaa aatcatctct ccataatagg
     2281 gaaagaaaaa atacgtccaa ttaatcgttt tttcctcata ggtttcttct atgcaacaac
     2341 tgaaaacatt tggaaatttt tctttaagcc attttttatg gtcattacta aaagtgtcac
     2401 tgctgcttac acataatgta ttatccagat ccagaacaat cgttggagcg ctattattgc
     2461 gctgaaagtt aatttttaag ctctttgaag gaatctcaat aagctcttcc tccgaatctg
     2521 gattgctccc aaagcaaccg gtaaagaaga gaacacacac gcacagaaag atttttttat
     2581 agatcatggg actgaactcc taatgggact cattgacaga gtatcttgct agaagaatag
     2641 aatagtcttt aaccttatta aaactaagaa gaaataagtg ccacgctttt ctctctcaac
     2701 acgtgctaat ttttgtgcgt attttcattt tctatttt
//
LOCUS       DAIAHG010000155         2682 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_155_length_2682_cov_2.731670, whole genome shotgun sequence.
ACCESSION   DAIAHG010000155 DAIAHG010000000
VERSION     DAIAHG010000155.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155_length_2682_cov_2.731670"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          687 a          508 c          549 g          938 t
ORIGIN      
        1 cggccactca ccccataaga tgtttcatat ttcgcccctc cgccaatact gcgtgcggga
       61 ccttctttga gatgaatcag aagcggtgtt ttctcatcag agctttcctt gggtgttagg
      121 gttacttcgt taaagagccc cgtttctaaa agagctttac gactggaatc cacgcgtttt
      181 tcgtcataaa gatctccctc tttataatcc aggcgattga gaacaaaacg ctcatcaaga
      241 tgtgtgaggc cttcaacttt tgtggatcca aaggttcctt tgggcccaag agaaatatta
      301 aaaataacga gaatggttcg ctcatgctca tttttcactc ccttcatatc caacgctttg
      361 gcaaagggat agccttttgt gcgccaatat tttgctattt tacgcccaag atcttgggct
      421 ttttcaaggt tcaccgcatc gcctttttta agcgaaagat tcagctcatc cagtcgaatc
      481 atatgaggag gcaggggacc attataatga atctcaaaac ctgaaaatgt gtaaagaggc
      541 cccggtgtaa tggaaaaata aataacgtaa aggtcctctt cttttttaat ctcaaagtga
      601 acttttccat caaaataccc aagtgtttcg agggtttccg taagctcctt cacatctttt
      661 tcggcgcgct tttcaagggc aaagggggtg aggggcgggc gcacttcaag gcgcttaagg
      721 atcgatgcat tctcaagttt tgtcttcaga tgcggctcca aatcacttgg aaaaattgtg
      781 atataccgcg cggctcgttc attgacaacg gcactttctt gcttttcttc caccttccaa
      841 aggttacagc tgcttaagat aaataaaagg gagaaaagta aaaaaaaacg catgcgagag
      901 agaaataatc aaatcttatt tcactctagc aaaaatcaaa ggaggcgggg aaagatttgt
      961 actcaaaagc ttttttaaaa ggcttaaggt cgtacattct aaaagtcccc ctgtctaaaa
     1021 gacaagggga gaagagattt ttactttaat taaaaagatt tttaaaactt ttgaatgtat
     1081 ttttcacgtt tttctcgaaa tctcttgtgt ataatttatt aagttcgtat tcagttttga
     1141 ttaaattgtt gtaaccaata gtaacgagat tttctagtgt gaatatccct gccttaatac
     1201 agttcgcagc cccaaggggt tgattgccgc gccacacatc cgttttttga ggatttgtgt
     1261 tcctcaacat catccatact tgcatcccaa aacgcacaac ggtgtcatct aaaatgttat
     1321 cttcagaacc gggtgatgtc ttagcgttat ttttgaaaaa agtatccatc ttgaactcag
     1381 agaacgattc aatatccaga taaatcttcc aaacattttc taaatactga gcaaaatttt
     1441 cttttgtagt attgtttccg ttcacaggga aatttgagtt tttacgggaa tcttgagaaa
     1501 aaataatgtc attaatgtgg atttttttaa taacctcttc ttttttctca ttttcttctt
     1561 cgattgtaat attgtttata ttgatgacaa cgtcattttt atttttttcg ttgtcttttt
     1621 cttcgctgat ttttttatat ttgtctataa atttttgaag tttttccttt ttcttctttt
     1681 taagctcctc ttgttttttc tttcgaattt gagctttaac gttttttact tcatcatcgt
     1741 ccgatgtatt ttcatcatcg ctagtacgtt ggtttttcgg aagtttttta tattcctctt
     1801 cctcttcaac tttcatcgca taaacatttg tgatcaatcc aaagaaaaca gcggaaagtg
     1861 caagggagag cgtaaaattt tttaataatt gtgtcattgt gaatctccat aaattttaaa
     1921 tgagggtgaa gtaccaaata atttgtgtct ttatacaatt tggcctacag tttaataaag
     1981 tgatttttag gtaaaagtaa agcttctttt tatgctggat gtgcgcacag ttttgttttt
     2041 gctgggagtg cttttaacac tcgtggcgac agcaatgctt cttccgctgt cgttagatta
     2101 ttttatttat gagacgcacc attggcaaag ttttgcggta tcggcctgtg tatctgggtt
     2161 tgcaggggcg cttttgtttg tgtcgaatca gccggatcat gggatcgagc ttggggtgag
     2221 agaagctttt ttgctcacag ccatgctgtg gggggtgagt tcccttgtgg cggcgcttcc
     2281 tttttatttt agcccattag agataaattt tgtggatgct ctttttgaat ccacatcagc
     2341 gttgacgaca actggtgcga caattctttt agggcttgat gggatgccaa agtcgatttt
     2401 gctgtggcgg gcgatgctcc aatggtttgg gggcattggg attatcgtga tggcgatgat
     2461 tatttttcca attttgcgca ttggcggcat gcagcttttt cgtagtgaat tttccgatcg
     2521 ttcagaaaaa attcttccaa gagtctcgca aattgccaca agcattttct ctgtttattc
     2581 aattctgacg gtgttgtgtt tgtttttcct ttatatggcg ggtatggaga tttttgatgc
     2641 tgtgtgtacg gccatggcaa ccctttcaac gggcgggcta ac
//
LOCUS       DAIAHG010000156         2593 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_156_length_2593_cov_3.314785, whole genome shotgun sequence.
ACCESSION   DAIAHG010000156 DAIAHG010000000
VERSION     DAIAHG010000156.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156_length_2593_cov_3.314785"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          677 a          598 c          494 g          824 t
ORIGIN      
        1 tgaaggatat tttgcgtgtt tttaagcgat atccccatta ccgccctaat attcacctcg
       61 tcgaaacaag tcaaaatctt caagacatac aaaaaaaaat acttttaccc tccactcact
      121 ctatctcttg gcacaaggat tttccaaaaa taatgggacc aacctttatc attgcaaatg
      181 agttttttga cgcccttccg cttcagcaat atttttttca gtatcaccaa tggcacgagg
      241 tacatgtgaa ttatgatgag gcctttcaca tcgttttttc cccggtagat caaaaggatt
      301 tacctataaa tgccgatgag ggggcttttt ttgagacatc ccccgcatcc catcactatg
      361 caaaaaaaat agccgattac ctccatcatc atgggggagc tgctctttta atcgattatg
      421 gcgattatat tccccaaaat cgcttcggag aaacattcca agcgctctct cagcatcgtt
      481 atgttaatcc ttttgactac ttgggtgagg ccgatctaac ctatcaagtg gattttgccg
      541 cacttgatag ccttttcaaa aaatactctt tagccacaca tttttcaaca caacgggatt
      601 ttttaaaagc acatggattt gaggagcgtc tttctaaaat actaagcacc tgtagtgatg
      661 cgtcgatcaa aaaagatcaa tacatcagag cacaacgcct tgttcatcca gaggcaatgg
      721 ggcaactttt caaagccctt gagattttta aaatttaaat aaaatataca attaaaaatg
      781 agaataatat tacaaataga cacctaaaat aatattctat tcttcaaaaa aggctgcttt
      841 tttgctataa ttcagcctgg aactttctta acaatgtact catgacgaca cccactcccc
      901 gcacatcgtg gcttcaacgc ttacttcaag gacctcacgc attcattgca acgattgtga
      961 ttgctttttg tgagagttct ttttttccga ttccccctga tgttggcatg atctcgatta
     1021 ttctccacga ccgcacaaaa gcatggcgcc tggccttttg gtgcagcatt gcatccgtac
     1081 tcggtggcct ttttggctac atgattggcg cctatttgtt tgactatatc ggacataaaa
     1141 ttattcatct ctatcacctt gaggagcgct ttttcactct ccaagaagac tttcatctgt
     1201 atggcttttg gctgctcgtg cttaagggct ttacgcccat tccttataag ctttttacca
     1261 ttgccagcgg cgttttccat tttagtcttt ggcaatttgt cctggcctcg gtcattgcgc
     1321 ggtcgggtcg gttttttgca ctggccacag gtttatggta cttggggccc gtggcaaaac
     1381 cttattttga ccgcttttta gggtggatca cttttgcgat tcttttgctg gttgtccttg
     1441 gttttattct tataaaattt atttaatcag cttttttaga aaatcttttt gctttttaac
     1501 cgcgacaagg tatcctgacg ataggtcctc tggtttgtga ttattgagtt attcagtgca
     1561 acgctctttt aaagatatcg cgcttcttat gattatagcg cttctttttg cctcttttgc
     1621 ggagcactat tggcacgttg caccctgtca ctattgcctt tatcaacgct atatttttgc
     1681 agcttcagcg tttttcttcc ttatcagacc tttgcatttt ttaggatttt tagcgcttct
     1741 tgccgggctc tgcttatctt tctatcacat aggccttgaa caccactttt ggtcggattt
     1801 tttaaataaa tgccagactc ctttggcaaa ttttgccaca gctgtagatc ttaaaaacgc
     1861 gattaaagag gcgccaattg tacgctgtga tcaagtaaat tggcggattt taagaatttc
     1921 cgcaacactc tggacagccg cattccaaat gctccttctc cttttatact gtatgagagt
     1981 tcaatgccgt gaccgaaaaa actaatcctc cattaagccc ctctcttcaa tccatgcttc
     2041 gcgtgaatca agcgggtgag tatggcgctg tgcgcattta tttaggacag atggcggctt
     2101 tgcaaaataa tcccaaagcc ctcaaaaccc ttgagcatat gctagagcaa gagaaagagc
     2161 acttagaggc ctttaataaa cttattattg actatgacgt gccacctacg cttttgacgc
     2221 ccttgtggca tgttggagga tatcttatgg gcgtgacctc ggctcttctt ggcgaaaaag
     2281 cggcccatgc gtgtacgatt gcagtcgaag aggtcattga agaacactat caaagtcaag
     2341 tagatgcgct gagtgcctca acacatccaa gagatgttca gtttaaagcg ctttttcaaa
     2401 aatgtcagca agaagaaagt gaacataaac atattgcaat agatcaaggc ggagaaagcg
     2461 ccccttttta ccccattttg acaaacttga ttaaaaatgc cacccgtctg gctatttttc
     2521 tctccaaaaa gggataacgc gggcatagtc ctgcccctgc ggggcccacc acgtccccaa
     2581 atttgatggc aag
//
LOCUS       DAIAHG010000157         2532 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_157_length_2532_cov_2.815479, whole genome shotgun sequence.
ACCESSION   DAIAHG010000157 DAIAHG010000000
VERSION     DAIAHG010000157.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157_length_2532_cov_2.815479"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          854 a          496 c          564 g          618 t
ORIGIN      
        1 tttgatgaga atttgctttt ttgtggcgcc catggttttt ccagcctcga caaggccttt
       61 ggggagggtc tcaaaaatat cctccgcaag gcgtatatac agcaaaatag ctgctaaaga
      121 cagcggaaga gtggccgcga gagtgccaat agatgtgccc acaataagac gtgtgagggg
      181 caacaggaga accataaaaa taatgtaggg aatggacctg agggcattaa taataaaacc
      241 atttactttg tgccaaaagg gtttaggtgt taagcctaca ggactgataa tccataaaaa
      301 aagagcaatg ggcgtgccta tgacaaaacc aagagcggct gagagtgtca ccatcgcgac
      361 tgtttctaaa aatcctttta aaatgagatc aatcacgtat ataccccaca cattctgttt
      421 tgacatgata atgattgaga taagactgaa catcatcaaa aatagtttgc gtataaggaa
      481 aactcatcaa aagctggcca aaaacagctt cgcgcacatg atcaaggtgg ccggaaataa
      541 tattgatagg aagatgaaat ttctccacaa gggaggcaat aatgggttcc tttgccgcac
      601 tattagagaa aagaagccgc acaatgatct gactcccttc caagggctca tggacgagac
      661 gttcttcata gctgtgcggc agttcgcgct taaaaagact gccgatcaaa gattgtgttg
      721 tttcatggat aggatgcaac aaaagcgtgc tcatggatcc tttttcgacg cattgtccct
      781 tttctagaac aaaaacggta tcacagattt ctttgacaac ggtcatatcg tgggtaatga
      841 gaataaccgt cacaccaagg tgcttattaa tatttttaag aagctctaaa atatcaagcg
      901 ttgtttctgg gtcaagcgca gacgtaaatt catcgcacag caacacctcc gtatccacaa
      961 ccaaagcccg tgcaatggca actctttgac attgtccccc actgagttct ctggggtagg
     1021 cgcactcttt ccctgtgagc cctacaagat ccaggatatg ggtaacgcgc tcttgaatga
     1081 gtgtttgaga aaaacctcta atctccaaag gaaaggccac attttcaaaa acagtacggc
     1141 gactgaggag attaaattgt tgaaaaataa cccctatttt ttgctgaatg ggcacagtat
     1201 ttttcttatt tttagacgca atatcctggc ccaagaggtg gattgtgcca gaggatgcag
     1261 cctcaagacc gcttagacag cgcaggaggg ttgtctttcc agccccactt cgcccgataa
     1321 ttcccgcaat ggatcctttt tctatggaca aagagatatc gtccaaaatc acagtgttat
     1381 tgatgatttt agaaagattt tttatatcaa ccgcgtggat cacaggtgtc aattgtttgc
     1441 ttactatcct ttaatgacag aactttacca tgcctttagc cttaagcaaa gtttttgtgt
     1501 tttttaaaac gatggagcca tatgctgaag agtattattt cccaaaatta agaaattttt
     1561 tattgaggga gaaactctgc gtagagttca gaaaagttct gtaaggaaag gtggagaaca
     1621 agcgcaagaa tcgtaaaaat aaggacaaac ccaagaagaa tttgcaaagg aagtgaaatg
     1681 aaaaagatct gaatattggg taccacacgg tttaaaagac ccagcatgaa ctgaaaaacc
     1741 acccctaaaa ttaaaaaagg aaggctaagc tggcaagcaa tcgtaaaact cttcgagaaa
     1801 aaaagaggaa gcttctccag tgagcttaag aaatgaccta ataagggatg atctgtgtga
     1861 atgggaaaaa aatgatagct ttgaatcacc gaccgaatga ccagatagtg aaggtccata
     1921 gcaaaaaaaa gcatcagacc gctaagaatc acaaaattat ctaaaacggg cgtttgtccg
     1981 cccatactcg ggttgaatac catggcattt ccaaggcctg tctgaagact gagaatattt
     2041 ccaagaacat cgagaatgct caaaagaagc cgtgcgataa acccaataat gcttccgata
     2101 atcgtttcct gaacgagcaa ggcaacaagg agtacgccat caattttctc ggatgtaatg
     2161 ggaatgggaa ggatatcctt aaaagcaatg gcaagggcta aggaaaaaag caaacgatag
     2221 cgcatgggaa tatgcgtatc gctcagccct ggcgccatca taaacacagc agagatccgt
     2281 gcaaaaatga gcaaataggg ctgaaccagt gcaatgaggt cgatggtcat tttaagacac
     2341 tttaatcttg tcaaataaaa attgtgtgaa ttccattaaa ttctgaagca tatagggaaa
     2401 agcaagggaa aaagttacta aaatagcagc gagtttgggg acaaagctaa tggtggcctc
     2461 ttgaatttgg gtgagcgctt gtaaaagaga aatgaccaaa cccacaataa aactaatgat
     2521 caaaacaggg gc
//
LOCUS       DAIAHG010000158         2380 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_158_length_2380_cov_3.036908, whole genome shotgun sequence.
ACCESSION   DAIAHG010000158 DAIAHG010000000
VERSION     DAIAHG010000158.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158_length_2380_cov_3.036908"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          733 a          482 c          497 g          668 t
ORIGIN      
        1 tagtatccct cgcttaagat gaagggatgc cgtggatctt ttagttttcg caagaagtta
       61 tgaacttctt tttaatgagc tcataaaatt gtgtcccgct aaatgaagcc agattaaatg
      121 ctttgtgcgt atcatttttc tcatcgtatt tttcctctat ctccttttta aatgcgttta
      181 attttttgat tagggattcg tattgcgtac actgagattc agttatcacc atcacctcat
      241 agaggtcttc accatttttg aatgttctcc ctttaagtac gtcaaatctt tttggagaaa
      301 gttctataat cttttttttg agttcttcta ccttattcct atccgcagag gaacctaata
      361 tagacactaa gagtgctgat attttttttt ctttttctaa cttcttacca taagcatgga
      421 taaaagagtc tagtttttcc atgattctca tttgcggagt aattatcttt acctttatta
      481 gatttttttc aaattccttt tctgctgcga tcacagatac agtgagagtc gaaaaaatac
      541 caaggcttaa aagtaatagt ttaagcttgt tgcttgttca tgctcctata tttattgcac
      601 tcatttatat ataggtatta tgaatagtat tcaatgcttt ttattcacat cgcgccagat
      661 tttacgcttt gccgcataaa aaatacctgt cattacaaga agatatatta ggaccgtaaa
      721 gcctaagcgc ttgcggtttt ccatctcagg ttcagaggcc caagaaagaa attctacaac
      781 atcgcgcgcc atctgatcga cggttgcttt tgtgccatca ctataggtta cctggtcatc
      841 ttggaggggt gggggcatgg aaatctgtcc ccctgacata taaggattat agtaacgtcc
      901 atcatcaaca gtcattcctt tgggaggtgt tttgccatac cctgtcaaaa tctgatacac
      961 atgatcagga ccattgtggc gtgccttgac aatgagtgac tgatctggcg ggagtgcgcc
     1021 attgtttgca gctctggcgg ctaaatcatt gggataaggc gacgggaacg catcacttgg
     1081 aagggcaggg cgctcaacat cctcaccggc atcattaagg gttttgacgg tatacgttgc
     1141 cgcaagagcc ttaatttggg gcgcagtaaa gccaagttct ttaagctctc gaaagcgaat
     1201 atgcttgagg gagtgacacg tcgagcaaac ttctttgtaa acttgaaatc cgcgctgaag
     1261 agctgctttg ttatacgtgc caaaaggtcc ttgaaagtcc caatgaattt tttcaataga
     1321 tggcttcaca aaaagaaatg aaaaaataac ttcgtgaaaa cctgttccca aaatgccaat
     1381 cagtgcaaaa aaactataga taaggatctt tttcattttt tattgatctc ccttgaggat
     1441 gggatttgca atgctctctg gcagaggtaa aacaggctca aatcgcgaga gcacaggcaa
     1501 gataacgaca aagtgaaaaa aatagtacat ggttgccaat tgacctatga gagaatacat
     1561 cccttcggga ggatttgcgc cgatccatcc taaaattaac acatcagtaa ttaacagcca
     1621 gtaaagatat ttaaaataag gccgaaaacg tccacttttg acaggatgac gatcaagcca
     1681 agggagaaca acaagaaggg caatcgcgct aaacattcct aagacgccca taagtttatt
     1741 ggggattgag cgcaaaatcg cataaaaagg gaggaaatac cattctggca caatgtgttc
     1801 tggcgtcacc atggggtttg ctggaatata attatcgggc tcgccaaaga aattgggtgc
     1861 gaaaaaaaca aagacgaaaa acacaataaa aaagaggcca aagccgtaga gatctttcat
     1921 tgtataataa ggatgaaagg ggagggtatc agcctttgtt ttcttctcaa cgccaagagg
     1981 attgttggat ccaaattgat gcagtgcaat taaatggagc attgtaatgg tgacgataat
     2041 aaagggaagt aaatagtgga gtgcaaaaaa acggttgagt gtaggtcctc ccacagaata
     2101 accgccccac aaccactgca caatgctttc gccaaaagga aaggctgaga acaaattggt
     2161 aatcaccgtt gctccccaaa agctcatttg cccccaggga agcacatagc ccataaaagc
     2221 agtcgccatc ataatcacaa aaatcacaac accaaggatc cacaaaagct ctcttgggga
     2281 cttgtaggaa ccgtaataaa gtccgcgtgc aatgtgtgcg tacacgacaa gaaaaaacat
     2341 cgacgcgcca ttcatatgga catagcgcat aagccaaccg
//
LOCUS       DAIAHG010000159         2374 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_159_length_2374_cov_3.587723, whole genome shotgun sequence.
ACCESSION   DAIAHG010000159 DAIAHG010000000
VERSION     DAIAHG010000159.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159_length_2374_cov_3.587723"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          650 a          395 c          504 g          825 t
ORIGIN      
        1 ggagttttca ctcctctcta tattattact tctatctttt tgaagcctgc tctctcgata
       61 atttcaagct gtgttttgat gctttctttt gaaatttttc ccttatggcg ctcaaaacta
      121 gtgtctatta gtgtcttttt gatggtttca taattttcct cgcaggacaa attcgcgtcg
      181 agggtcaacg ttaaagaatc gctaatgatc gtgtacttgg ggaacttttt gtccaaaaac
      241 tttttcatat tgcttatgaa cggagcaatt ttatcgtttt tattgttgca taagaccttt
      301 atagcgtgca tgagaattat gttttcctca tttttatcat ttttttcatc ggtgcatatt
      361 ttaaaaggtt ttatttcagc ctcaaaagcg gttttttgac tgttgatttt tgtggctagg
      421 cgtttttttc gctcattaag agagtctttt cctgccattg cgacattcat cgtacagaga
      481 ctcactaatg atgtgctaag aaaaattaag aatatgtttt taatgttcat ttttaataat
      541 tagtataagt ttttattaat caataatgta ataactattg ctttgtgatt caattgtatt
      601 ttttcaataa aaatataaaa aattataaaa tgattttatt ggttttttat gcaaattatc
      661 ttatgatagg aattggaaaa attttaggag ttttttatca atgaaaccca tttttattgt
      721 tacatctttt tgtcttttgg cgggctcttt ttctttggcg atggcggaga aaaataaagg
      781 ccggtcaact gtgaatccag cgattccaga cgcctcacaa aatacagaaa atcaactgac
      841 agatccgtca ttgacagcaa aaattataga ggctgaaaat cgtttaaaaa aaacaaaaac
      901 attttttatc gataataaaa gtttggaacg tgttttcgaa attcttaata atcaaacccc
      961 gaccaataat caggggcaga ttgatcaaga ggagaacgcc aaggaagtta cggtgtcctc
     1021 caatggtgtt tctggcgtta agtgtattat gaatggcaat tgggcggcga cggggactgg
     1081 taaggatgtc gggaaaagtg ctgcgggatg ggtggatgcg agtggaaaaa gtattctgtc
     1141 tcaggctgta gcagcgctcg ctggtaataa agatgtggca cgtatttctg tgccaacctc
     1201 tataaaaagc cctattggtg gtgctccgat taatggcaaa aaagaaatta tgtttgcgtc
     1261 ctccaaatat tttggtatgg ataattcagg tcggaagttc ctttgttttg ttgaagttga
     1321 cggctaatta ttgctggacc aactcttttt ataaagccgc gacatcccct cgcggctttt
     1381 aacgacgtga caaagaagga attaggcgac gatatcgatt ggtgcctcac cttcaggcat
     1441 tttttgaata tcatcgattt ggagaccctc ttgaggctca ttataaagaa tatcaagggc
     1501 tttgacattc ggtttccgtg tggttacagg cttttctttt aaggccatgc gctgaaggtc
     1561 ttcgccctct tgatgaattt tttgtgactt tttctttttg tgcaatttat catcagattt
     1621 ttgaggtgtt tttttatgct tggaaggcgt aaaatttgca tgctcgagca tttcctcatc
     1681 attttcttct ggaaaaagat agtcttctgt catgttttcc tcagtcatag ccggggatcc
     1741 agcaaggggg cgcgcatttt tttgttgtac agtatcaagc atttctgcgg atacaagggc
     1801 cgacatcagg ggtagtgacg tcgcaaaaat aaaactccaa agaattcttc ccatctcaaa
     1861 cctcctttga tgttataggt ctctatggga ttattttaag ggattaaaag ttaacaaatt
     1921 gtttaaggaa gcagtttttt ttgaatttct tcgttatttt tttgatgaat aagcattatt
     1981 ttataacgat ccgtctgtcc tttgagcagg gaaatggagg atttcgggat atccatttta
     2041 tcggctaaaa aagtaatcag cgccttgttt gcaaggccat cgacgggggg tgctgtcaca
     2101 gaaattttaa gaaaagcttg accttctgga tcgtaataaa ggccctggat gcggttatgg
     2161 gaagatttag gcgtgaggcg gacaaaaagt tcagtcccct ctttatgcgg gcgccaagga
     2221 taaaaactca taaagggatg cttatagctg tgtgtatagc cattttccaa aggcttcgtt
     2281 aggattacaa ggaaaactta aaagggcagg gcattcatag gaatgatttt ctgtaataag
     2341 ttttttaagc gctttcgaat gcgttgccat cgtt
//
LOCUS       DAIAHG010000160         2369 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_160_length_2369_cov_2.393106, whole genome shotgun sequence.
ACCESSION   DAIAHG010000160 DAIAHG010000000
VERSION     DAIAHG010000160.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160_length_2369_cov_2.393106"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          618 a          443 c          562 g          746 t
ORIGIN      
        1 cacaagggac atgacgcggg gcttgagtaa gtcgatatag tcaccgacag aaggcgtttc
       61 aagggtactg gagggaaagg aaagtgagga caaacaaata acctcaaggc taaaatttaa
      121 tcaatttata tactatttta cataaagttt caataatttg caaaaaagat aaagcgtttt
      181 tctgcattta atcattcaat tttataaaaa tttcttgagt tttcagggcg agtctgcttt
      241 cttaaaaggt gaaacttaga cgaatgattg aaaaataaaa aaatatggtc cagtttacac
      301 gacgcgaatt tagtttggcg gctttgtcta cagcggctct ccccctttta tcttgctcaa
      361 aaaaagcgtc cgccgatacg ttgagaattg gggttctttt gcctacgtcg ggccacgagt
      421 cagcgcttgg acaagagtgc aaaaagggag cggatattgc ttttgacgtt ttaaagcctc
      481 atttgggtgt ggactttgag cttgtttttg ccgatacaga atcgaatgca aatgtggggc
      541 gaacaaagac ggaaaagctc attaatagtg gcgtgcatgc gatcgttggc gcctatgatt
      601 caggtgtaac atctgttgta gcccaggtct gtgagcagcg tggtatgccc cttgtgatta
      661 atattgcctc agcgcctcag attacagagc agggctatcg ctatgttttt agaaacttcc
      721 ccacaggtcc catgctcatc cgaaatggta tgagccttat gaaggatcta tttcgggaaa
      781 aaggaatttt ccctaaaaca gcagccttta ttcacgtcaa tgatacctat ggcgaagtga
      841 tgcgcgcagg tatcgaggag cttatgcccg aactggacat gccttttaaa attattcaaa
      901 ccattgctta tgatcctaaa acacaagatt tatccacaga ggtgggaaaa ataaaagcga
      961 gtggtgctga gctgattatg cctgcgacac gtcttgatga tgctgtcttg atgattaaag
     1021 agattttaaa gcaaaaattg aatgtgatgg gcgtgattaa ccctggaagt cctgggatgt
     1081 atgaaaaaga attttttgat cagcttggtg acaaaagcaa ttactggatt tcgaatacgg
     1141 cgtggtacaa tccaaaagct agtttgtcaa agtctgtgct tgaggtttac aatcgtatgt
     1201 tcccaggatg cttttttgaa acgagtgcgg cctttacgtt tgaagcgatc catattattt
     1261 gcgatgctgt ggcccgtgga aaatcaaaag agcctaagat tttgcgtgat atgattgcgg
     1321 caacatacat tgagaatcat gtgtgtcttg gggggcctat tcaatttgat agtaaggggc
     1381 aaagtaaaaa tcttcagtct gcctccatgc aaaatctgaa tgaacgccca acggtgatct
     1441 tccctaaaaa tgcccgggaa gcagagccga tcttccctat tcctctttat catggagctg
     1501 cttaaatttt atgatgacac tcattaatgt tggtgtgaat ggtttgctta taggtcttgt
     1561 gtatgggctt atggcgctcg gactttctgt aatttttggt gtgattcgtg tggtgaattt
     1621 tgcccacggt gaaatgatgg tgataggcgt ttatggcgcc attattctcc acaaactttt
     1681 agggcttgat cccattcttt ccgcaccgat tttagccctt gtgctgtttg gattgggcta
     1741 tgggttgcaa agggggttga tcaatcgtct tttagaaaag cctgagcatg tgcaatttat
     1801 cgttcttgcg tctgtggcgc ttattctttt aaacctccaa ctgattattt ttggccccga
     1861 agccaaagcc gtaaatgttt cttacagcct cgaatctttt gaatttggag ccattcttat
     1921 tgataaaacc cgtgtttaca cagcctgtgt ttctcttctt gtcgcagtag gcctttttgc
     1981 ttttttcaaa tatacgctga tgggaaaaat gattcgggcc tgtgcagata atcttttagg
     2041 tgccaaggtt gtggggttga attataaaca cttgtacgcc atgagttttg gtattggtgc
     2101 tgggtgtgtg gcgattgcag gaagcttgct cagccttatt ttcaatgtga cgccgcactt
     2161 agcgcctgaa ctgactcttt tagggtttgt gattgtcatt attggaggcc ttggaagtat
     2221 gccaggcgct cttttcggag gtgttcttat aggccttgtg caaagctatg cgggttattt
     2281 tacagagcca tcgctcaaat cgctctttag ttttgctctt cttatcattg tacttatgtt
     2341 caaacctcaa ggattattta aacaatcat
//
LOCUS       DAIAHG010000161         2307 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_161_length_2307_cov_2.896861, whole genome shotgun sequence.
ACCESSION   DAIAHG010000161 DAIAHG010000000
VERSION     DAIAHG010000161.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161_length_2307_cov_2.896861"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          646 a          438 c          527 g          696 t
ORIGIN      
        1 gcgggtaaaa tagcttgtct tccagctgca atgacgaggt ctggaaaagg gccttttaag
       61 gaatcgctct cttttgtaaa ggcatgagac gcgccccaca ttaaataagg tgaggtaaga
      121 tgccaaaaag ggcgcaaggt cactttttta tgggtaatca caacaggaag atgctctgct
      181 aagcccagtg cttgattaat aatgcccgca taatgattcg tgataaccca acatgttttc
      241 attggtgaga aattctcatt tttggagcac gcattacgtt gctaaatatt ttatctcttc
      301 atatcacaca ttatagtgtg attcatagga tagaagaaga gggcggattt tagtaaatta
      361 gcggggggga gattaaaaaa attctccctc caagcgtttt cattcataga ttacaaccac
      421 acttctttgg cgacagcagc catctcgtgc agcttagcgg gtgagaatag acctgagaaa
      481 cgcgcataaa cctctttgat tctttcatag ggaacaaggg aaaaaaggtg atcgagaagg
      541 tgaataaaat ttttatctct ttgagaaagc ttttgagaaa ggtgggtcca cgccttttca
      601 aggtgttgat cttctttttg gaatgacaaa gagtaatttt tttgatggct tgagggcaca
      661 ggcgagataa aagactgaca cccatcgata tgcggtttaa gcaaacgcaa atagcgacgt
      721 cttagaatat aaattttatg cagcggctca agatcatcca aaagaagggt tctctctttg
      781 agtaacatag aagcgagtgt ccctgtgagt tgtaactctg aaagatcgac tccttgacgt
      841 ttattttgaa gctcaagtgt tccaaagtct ttgctgggtc tatttttgca aggtcttcca
      901 cgcttgcgat acgttgtagc aagcgcaagt gttgttgttc ttaccatgta atatcctcca
      961 caagaaaaat cgttagtttt ttttcaaggc gtattttatt ggcacacatg acgatctgca
     1021 tttttgacaa aggacgtcct tctaaaaaat aagagtcatc agaggggtga gaaacctcaa
     1081 tgccttcctc tttcagccac gcaagaatgg cattaaagga tgtccacgca agacatgctt
     1141 tttgattgac gtccttgtat aattttttcc gtggtttggt gggctccaaa cgcacgggtt
     1201 tttttaaaag acgcggggtg cgaatacgaa aacgactaag agccttattc acagaaccag
     1261 gggtgcgctg aagcgctcgt gcaatcattt taatcgggag cccttttaaa aaatcagtgc
     1321 gcagtctttc aatctcatca tttttccaac tcatacaggg tgtcatcagc atctccatgg
     1381 tctttcctta caagaggtat gtgttttttt catcattacc agtctctttt cgcatatcta
     1441 ataaaaaaaa taaaataata aaaagtccct ctttttatta ataaaattat aaaatgagaa
     1501 atcatttaag ggatgcgccc cctttttgtt tgatttatag tcatgctggg taaatgctta
     1561 aaaaacgagg gaacggcatg tcttttcaca ttcatttttc ttctccagcg gaggaaggcc
     1621 tcgcccctta tcttgtggaa ggtctctcca agcatgcaaa ggaaaagcgt ggtttagcag
     1681 ggatgtctga tttttctttg acggtaaaaa ataacaaaga tgaggtgaag ggcggccttg
     1741 atggcattct tttttatgga tacctttacg ttgggaatct ttatattgat gcggcatacc
     1801 gcggccaaaa tatcggcaca gagcttatgc acaaagctga agcttacggg aggtcaaagg
     1861 gatgccgttt ttccactgtg aatacaatgg attttgaggc gcttgatttt tataaaaaac
     1921 tgggctactg cgttgatctt gaacggcacg ggtttgacgg aggatcggtt ttttattttc
     1981 tgatcaagga actttgatgc tcattctttt gaacggcgct tcctcaacgg gaaaatcctc
     2041 gattgccaag gctttacagc atttatccga ggagcctttc ttgcacatag gtgttgatac
     2101 attttgggat atgatgccac ggcactatgt ggatgcgcct cgttttgagt cggaggagga
     2161 taagaaaacc acgtctttcc ctaaagttga agggtatcct cacaacgttt ttcaagcgat
     2221 tccaggtgtt ctaaaagcac ttttgggcgc tcatttgaat gtgattatgg atgatgtgat
     2281 ctgtggcgat gcagagcgac taaggac
//
LOCUS       DAIAHG010000162         2306 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_162_length_2306_cov_2.990130, whole genome shotgun sequence.
ACCESSION   DAIAHG010000162 DAIAHG010000000
VERSION     DAIAHG010000162.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162_length_2306_cov_2.990130"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          733 a          507 c          405 g          661 t
ORIGIN      
        1 tttgagaagg ggacttgggc tagagcgatt taaaataata taaagataag accgctcaag
       61 agaggaaaag cgtgcatgaa aatcaggcga aacctctttt atatcgagga tagaaactcc
      121 tttatctcga ccaaaatgat taagacaccc cttgagttta aagggatcga gagtctcgtc
      181 actatcaaaa tgcgccactt gacccaaggc atgaacgcct gtatcagtgc gccctgacac
      241 atgaagtgtc acagatttct ttgtaaaaat accaattttt tcctctaaaa aaccttgtac
      301 agagatttga tgaatctgcc gttggaatcc agaaaaaaaa gaaccatcat attccatcaa
      361 cagagcatag cgcggcatga ttaattatga gttatgatta tctttctctt tttagcgaat
      421 tttcttaagc atagacaagg cttataaagc tatttatata aagagataag ctcaaaaatt
      481 tttttaaaat tcatgtcaag aaatctacgc tcaacaatca cacaaaccac gtgcttttct
      541 catattcaac gagaaacagc acacttaact gaaaaatctc ttgttatttt tgatattgac
      601 gatgttttag tcacagcaaa agacaatgtc ctgaggggtt caaaaaaaga aaaatggttg
      661 gatcactatt ttaatagtct gacaccagaa gagtctttag agatatccag ctatgtatgg
      721 acggcctatg agacggaaat taccgatatt caagttgcct attttaatga ggaccttagg
      781 gcacgaaaca tcccctccat cgcgctcaca aaaggttgga atggttcaat ggcaaaccgg
      841 ccgtcccact gcgatgatcg cattcaaaca ctgaaagata ttggcattga ttttcgttat
      901 tcttttccaa ataccccaga aatttttcta aatgggctcg aaaactgcgg aaagattcct
      961 tcttttaaag agggtgtgat tttttcgtgt atgctctcta aagcgattgt tttggatgct
     1021 tttttaaaag ccgttgactt taaacctgat gaaattatct tcattgatga catgctcaat
     1081 aaccttgagg atattcaaac ctactgtgaa actcatgccg taaattatca gggatttcac
     1141 tatgtaggcg tagatgcgcg agaaacgcaa cctcttaatg aggagattgc ccacctccaa
     1201 tttcaaacag ctttgcgtga aaaaaaatgg ttaaacgatg cgcaagctca tcaaaaactt
     1261 ttacataaac ctaaaagttc tcaagaataa tgagtgatat agagatttaa atctttaaaa
     1321 aactaatctt taaagcgctc tacgtatgtt ccgtctgctg tgttgacaac cacgcgaatg
     1381 cctgcttcga tgtggggcgg aaccataatt tttaaaccat tctctagcac tgcaggctta
     1441 aaagatgaag aggccgtttg ccccttcaca acgggatcgg cctcgacaat ttccatcacc
     1501 accgtatcgg gcaaagacac cgtaatgggc ttaccctcat aagagctgac atcaacaatc
     1561 attccttctt tcagaaaatg cacagcatcc ccaataaaat cttttgacaa ggtaatttgt
     1621 tcataggttt gctggtccat gaaaacaaca tcatcaccat cttcatacag atattgataa
     1681 ggtagctcgt ccaaaaacac acgctccacc gtttcagcag aacgaaaccg cacattcgtt
     1741 tttgtactcc ctacgagagt tttcatctcc acctgcatgt aggcaccgcc cttgcccggc
     1801 tgtgtatgtt gtgttttaag cacgacccaa agttttcctt caaactcaag gacattgcct
     1861 ggacgaattg tattaccatt aattttcata aaaaattact ttcttaaatt attaaactag
     1921 tttttaaaaa tattaaccat tttatcgaga atatttatag catcttcacg ctgtctttga
     1981 aaaatatttc gccccacaat agcaccaaaa ccaccacctt ctttaatcgc cagagtatct
     2041 tgaagcaact gcgcatccga tttcgtttcg ccaccggaaa aaataacgat acgttttccg
     2101 acaaagcagc agtcgcgcac atgcttcaca cgctcctgaa gcgtttcacg aggaatattt
     2161 tcgtaaggtg actcatgcac cttcattgca attttatttt caggaagctt aaccttcaca
     2221 atgtgcgcgc caagaagaca tgccatatgc gccccataac taataacatc gagcgctgat
     2281 tcattttcct tagcaatatc acctcc
//
LOCUS       DAIAHG010000163         2299 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_163_length_2299_cov_3.716022, whole genome shotgun sequence.
ACCESSION   DAIAHG010000163 DAIAHG010000000
VERSION     DAIAHG010000163.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163_length_2299_cov_3.716022"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          695 a          469 c          441 g          694 t
ORIGIN      
        1 aaatagtgca aaagagaagg gagttcttcc tctggggggc ctgcctcaat gtgaatattt
       61 ttctcagaaa gttttttgac aagatccgga tcaaacggtg catatgtcac aaacacctgg
      121 ccatcttttg tttttccaga gatatgttgg ccgcgaatca tgacatcgct gactttacta
      181 ttatccacat tgtgcagaaa ttcagaaaaa gcaacgggat gagcgttttc cttgcctttt
      241 gcccctggaa gcatttggaa aagtccaaaa agccccacgg caatgagcat ccaaataata
      301 aagtttcgtg tcgtgttatt caacgcctgt cgtcctctta aaaaatgtct ctagcgattt
      361 tttttatata aaatcataca gctttagttt tacaggattt ttcttaaaga atcatgaaaa
      421 aagacacaaa agaaggtgaa aaatatgcac gaatggaaaa tctttttcac attttattct
      481 gatccataca aaatcctcaa atcaaagtaa ccattgaagg ggagaaaatc tttgatccca
      541 atgcgatttc tattgcgctt taaagctttt tggatccttt cgcgttcttc ttcagtatat
      601 cccaccgttc cattaaaact tgtaagggaa gaactgcgtt cgatatattt aaggattctt
      661 ttccgactcc cctcgtcttc ctcaaagggg tttccataaa gacccaatgt tgcgcaatca
      721 ctgttttgaa tgaggtcaaa cacatactga agaccctctt tatcgacatt gttatatcca
      781 agcaacacgg tctcaaaatt ataatgacca agcctttcgc tgacccgctt catcccctca
      841 ttacccataa attgtgcata aaaattacaa tcgggaaact tatactttac gtgcatctcc
      901 tcaatctcat taattttttc ttccaattgt tttttgaagg actccttttc caatgcttca
      961 ttagaattag acgcagaaag acgcagcgtg gagaggatga taaaaaggag tgatgtgatc
     1021 aattgtctca tagctctcta ccacatgcgc ttcaataaat tttctctatc aaagatcaaa
     1081 tgctttagaa atccaaggaa atggagtgtg atcaggccca aaagcgcata tccaaaaagg
     1141 ctgtggatct gccacgcaag atgggaaaga tgtttatttt tttcaataat caaggggatg
     1201 tttacaccaa accacgcaac gggatgcccc cctgcagagc tcatgatata gccactaagc
     1261 ggaaacccaa tcataagaac ataaagacac gcgtagacaa tttttgcgag ggtacgctca
     1321 taacggcgaa cttgtggcgg cattggcggc acatccgaga caaggcgaat cacaatgcgt
     1381 aaaaaagcca ggccccatac cgttacgcct aaagatttgt gaatatcgaa taaattatat
     1441 tgaagaggtg gttttataag ctctgccatt gtaaatccga tgatcaaaag ggcgatcatg
     1501 agaagggcga tgagccagtg gagaacgcga agtgtcagtg gatatttcat atctttttcc
     1561 tctctgtgaa tgaatttgaa ctgtattaaa caagacctgc ttgtcaggaa gtgttgtttc
     1621 tatcccttcc tctgctgcat tattggtggg attgtttaga tgtctctcat aaggataagt
     1681 aatgagatcc tcacggtctt tttcattcat aggaacacac gctgtaagcg acaagcccaa
     1741 tgctaaaaat aataatattt tcataaaatt ttctccttcg acctttatgt taaaggaaaa
     1801 aaatgtgtat gtgaagaaag ttgtaaaatt atagggggag agaatgcctc tctcctaagt
     1861 gctatccaat agtcatagca agtgattttt gattatcgac aatgtaaggg ctatagtctt
     1921 tatttccaat atcataaata tcgtcaaaaa gaatcttctc attcagcact gccttcgtaa
     1981 gcgtcagttt attgagcacc tttgcataag ccttcggatc cttaagctgt tttctcagcg
     2041 ccttgttaac agtcatataa aaaaatttat cttttatgac ataatagtga ttttctgtgt
     2101 ctgaataggt gcgaaatgcc aaagatgtcc gcatcgaaag cgtatgcgat tcgttcttgt
     2161 catctgttaa ggttacatcc caagtcgtct ctaaatcatg ataggtttga aaattcttca
     2221 tctctcggcc ttttgtgggc acgcgaaaac ctatttcttc tggccaatcg agtttggtag
     2281 aggcccttgc gtctgcaaa
//
LOCUS       DAIAHG010000164         2220 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_164_length_2220_cov_4.443770, whole genome shotgun sequence.
ACCESSION   DAIAHG010000164 DAIAHG010000000
VERSION     DAIAHG010000164.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164_length_2220_cov_4.443770"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          589 a          444 c          531 g          656 t
ORIGIN      
        1 cacccacgtt cttgaccttt taaaaagagt tgggtttccc gaagccaaag atcgtcttca
       61 cgcgtaccct catcaacttt caggaggaca gcggcagcgc gtcatgattg caatggccct
      121 tgcctgtcgc cctaaaatcc ttattgcgga tgaacctacc acagctttgg atgttacgat
      181 tcaagcaggt attattcgcc tccttcggga tttgcaaagc gaatttaaaa tggcgcttat
      241 tcttatttct catgatttgg ggatggtcaa gaaatttgca aagggaaaag attcaaccat
      301 tgccgtgatg catcaaggtg agatcgtcga gtctggtgag attcagtccg tgctttcaaa
      361 gcctgcgcac gcgtatacac agcatttaat tgcatcagaa cctcaaggat cgccgttacc
      421 actgcttaaa aatgcaaaaa aaatactttc ctgtgagggt gtgagtgtta cttttggtaa
      481 gcccaagaca ttttggcatc ctcatggccg tgaggtgaag gcggtcagtc atctctccct
      541 tgatttgcat gagggagaga caattgggat tgtgggtgag agcggttctg ggaaaagcac
      601 actcgcctat gcgctgctgc gtttgcttac gacaaaagac aaaggtaata ttatttttca
      661 aggagagtcc ttaagtcaaa aaacagaaaa agagctgcgt gcgtatcgcg cagatcttca
      721 agttattttc caagatcctt tttcttcgct gaatccccgt ttttctgctg cacaaattat
      781 cggcgaggga ctgcggattc acgaacctca attaaccacc caagagcgac gtttacgtgt
      841 tcttcagagc ctcaaggaag ttggacttag tgaggaacat tatgatcgct atccccatga
      901 attctcaggg gggcagcgcc aacgcttagc cattgcacgg gcattgattt tgaatcccaa
      961 agtcattgtc cttgacgagc ccacatctgc acttgatcgt tctgtacagg cggaggtgat
     1021 tgaccttttg cgcaatcttc agcgtcatcg aaaactgagt tacattttca ttagtcatga
     1081 ccttaaggtt gtgcgcgcca tgagccaccg tgtgcttgtg atgtatcaag gggaattaat
     1141 cgagtcaggg gctgtggaga cgatctttaa tgcgccccaa aaagcttata cacaaaaatt
     1201 gctcgcagcc gctctttaaa aatagtgaaa ataagctaat ctttaaggtg aggtaatttt
     1261 acaaaagata aaccctatgc ggcttttcat ttttattctg gtgatttttt taatctttga
     1321 tgctgttgta catccaaagg aggcagagac gggggtaata ttagctgaga agcgaagaga
     1381 gcttgttgag gaactaaaaa aagaggggat tacggaccgg aacgttttaa acgctatggg
     1441 caatcttcca cgggagattt ttattaagcc cgctcttgaa aagcgcgcgt atgaaaataa
     1501 tcctttgccg attggtgagg ggcaaacgat ctcgcaaccg tatattgttg cgtacatgac
     1561 ccaggcgctt gatattaaag atggccaaaa aatattagaa ataggcacag ggtcaggtta
     1621 tcagacggct attcttggtg aattgaatcc aacgggtgat atttattcga ttgaaattct
     1681 tgaggatttg gcgatgcggg ctaaaaaact tttaaaggat ttaggctacg atcatattca
     1741 cgttaagtcc ggagatggct ataaagggtg gcccagccat gctccttttg atcgtattat
     1801 tgtcacagcg gcgccacctt ttatgccaaa aacgcttctc agtcagctta aaataggggg
     1861 cattcttatt gctcctattg gtgaagcagg ggatggtcag tccctcatac gcatcgttcg
     1921 tgtatctaaa attaagtttc aaacggaaaa attgcttggc gtgcgtttcg tgcccatggt
     1981 ttcagatacc gtagacggtg atcatcctta aacattagtt ttttgtcttt gttgctaaga
     2041 agtttctgcc tttttcgtat atttttttga ggctgaaatt tttttgggta ttggcttttg
     2101 tgttcacatg ttagtttttc aattttgagc aaatatttcg cgggaatttt tcacaaaaat
     2161 aattgtgatg gagggagctg aattcaaggg ttcttgtctc cttaaggggt ggtggtacgg
//
LOCUS       DAIAHG010000165         2197 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_165_length_2197_cov_3.210849, whole genome shotgun sequence.
ACCESSION   DAIAHG010000165 DAIAHG010000000
VERSION     DAIAHG010000165.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165_length_2197_cov_3.210849"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          617 a          462 c          419 g          699 t
ORIGIN      
        1 ctaaaaatag cctttttaac accataaaat ccccatctgc atccccccat aaagcatttt
       61 gacaccacta gaaaaccgcg tcaaagggcc tttttcaaac agcccttcac tacccatttt
      121 tgccttttct ctcctctatt atttattaaa aatcacacct aattttttga gctccatttc
      181 aaagattttt aactattttt taagaaatag tgttttaaat tttattatag acatttttaa
      241 aaaaaggcta acttatggca cgacttgcgc ttgttacagg gggaacacga ggaattggtg
      301 cggggattgc acgttctctc aaagaaaaag gatataatgt cgttgtcact tacgggggca
      361 acgctaaaaa agcaaaggaa ttttccgaat caacgcatat tccctcctat caatgggacg
      421 tttctgattt tcaagcctgt gcctctggca ttgcacatat tcatgaaaaa tatggccaga
      481 tcgatatcct cgtcaataac gcaggcatta cgcgggatgg ttttcttcat aaaatgacgc
      541 ctgaaatgtg gggcgatgta attcaaacga acctgacatc gtgctttaat atgtgccgcg
      601 ctgtgattga aacaatgcgc aaccagggtt ttggacgtat tattaatata gcgtcgatta
      661 atggtctcaa agggcaaata gggcaaacaa actattcagc cgccaaagct ggcgttattg
      721 gttttacaaa agctctggcc cttgaatcag cgaataaagg ggtgacgatt aatgccattg
      781 ctccaggtta tatcgataca gatatgattc aagctattcc aggatctgtt cttgatcaaa
      841 tcgtcgcagg tattccctca aaacgcctcg ggacagcaaa cgagattggt caagctgtgc
      901 ttttcttagc cgatgagtcc tcaggcttta tcaatggcgc aacgctcaat atcaatggcg
      961 gacaatatat gagttaaagg aaaaaagaag actcttgact taaggtttca ttgttctaca
     1021 atgtgctgat ttatagaagc ggagatcacc tagaatggac gccttaaaaa aagcagaatc
     1081 gtattatgtt gcgatgtgca ataaaaattt tgacgagatg gcttcttatg tccaccctaa
     1141 tattcatttt gtagggccgc tctcgactat ggatggaaag gaaaatgttg ttgaagccgc
     1201 taaaaacttt gccatttttt ttaaaggtct tacggttcgt gaaaaatttc attccaccga
     1261 ttctgaaaac aaagtgatgc tcgttttaga ttttgattgt ccggcaccta ttggtctttt
     1321 caaaggtgca tcctttctga cttttcatga ggggcttatt tcccgtattg agctttttta
     1381 tgatgcgcgc ccctttgaaa caaaaaagga tcaaattttt aatcaagaat agaaaacttt
     1441 ttttagagtt ttattttttc ttaatctcct cccacaggag gagcatctct tccattgttt
     1501 ttccatgcaa tgttacaaag cccttttctt tggctttttc ttttaaagcc atcatgcgtt
     1561 ttgaaaattt ctcacctgtt ttagcaagcg tttcctccac atcaaaccct gcaaaaatgc
     1621 aaagaccaag ggctgcatga atcacatccc caatttcttc ttgaagcttt gagggagacg
     1681 cttgcgcctt aagatcttct ttaacctcct cgcactcacc tataatttgc tcaaaaatca
     1741 tatcatagtt cggccactga aaaccaaaat cacgtgcatc cttttccaga agaataagat
     1801 actcaagcgc actcattttt gtcttttcca atgacatgtt aggttgctca gattctaaaa
     1861 cagcacgccc cctttgttgc gtttttcgct tcgcaaggtt cgtacgcaac gctgtactca
     1921 gatttttata acgattattt ttagaagtca ttaaagggtt tttccctcgt gtttatgatt
     1981 gctttgcaca ctatagctat gtcatagtgt tttcgtcaat tttgccgtcg tagctcagtg
     2041 gtagtgcaca cccttggtaa gggtgaggtc gagagttcga tcctctccga cggcaccatc
     2101 aatacatgat ttcattttgc tctggctgta aaatgcactt gtacggagga cattctttct
     2161 actgatagcg agagagtata ttttctttaa ggggaga
//
LOCUS       DAIAHG010000166         2165 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_166_length_2165_cov_2.788793, whole genome shotgun sequence.
ACCESSION   DAIAHG010000166 DAIAHG010000000
VERSION     DAIAHG010000166.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166_length_2165_cov_2.788793"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          651 a          437 c          464 g          613 t
ORIGIN      
        1 tgttttctaa agtagtcaat aattatcaca ataactagtc ttttcatttc atgcttcaaa
       61 caatgcgttc ccatgcggga agttggttca ttaagttttt actcgctgcg attattgcaa
      121 gctttgcctt ttttggcatt ggcgacatta tcaagggcta tcagcacggt cggcccgttg
      181 caacagtcgg cggctatgcg attggtatgg aggagttctc ttatgcctat aagttggcgc
      241 ttacaaatct tcaacacacc gccaaaaaag cgattacgcc agagcaggct aaggagcttg
      301 gccttcccca gcaggtttta gacaatctta ttaataaagt gatcttggaa caggaaatga
      361 cacgcttggg cgtggttgtt tcagacaaaa cgatcataga agaagttcat gctatccctg
      421 cttttttaac gaaaaatggt acgttcgatc atgcgatgtt cgagcaaatt attcatgcac
      481 aaggcctaag cgagggacgt ttcattagtg aggtacgtaa agaattgcaa cagcaccaag
      541 tgattggcgg gctgacgagt cctttgaatt tatcagatgc atatgtacgt attttatttc
      601 aagcgctgga acaacctctc caatttgtca gtgtggatat tccttttaaa aaaattcctc
      661 ttcaaaaaga aattaaaatg gcggatgtcg aactttttta taatgcgaac aaagagcaat
      721 ttagggtgcc tgaattgcgc acgatcacac ttttgcgctt tgatcgcgat gcgcttaaaa
      781 agcaaatgac tgtatcagaa gacgctatca aggaagagta cgagcgtcgc gctggagatt
      841 ttgcatcccc tgaaaagcgc accattgaaa gcattacagt cagcacaaag gatgcagccc
      901 aaaaagctat tgatgcgttt aacatgggca aacctcttaa aacaattgcg cgtgaattaa
      961 agggttctta taaagacttt ggctctgtga caaaagatca gtggcctgta gacgcgcaca
     1021 caaacgccct tttttcactc agtcttggaa cgccttctga aatgtttgat acagatcaag
     1081 ggtatgtgat ttacgttgtc actaaaattg ttccaggatc ccaaaaatct ctcagcgagg
     1141 caatagcaga cattgaatca tcccttaagg atgacacatt taatgagtat tttgagtcgc
     1201 ttcaaaataa agttgaggac gatcttgcgg gtgggcttaa aactcaagaa gtggcgagta
     1261 aatataatct gacgattgaa acgctcaaag ctgtggacag aaatggccat gatagtgatg
     1321 gtgcgaaagt cctcgaggga agcactaaaa aagatatttt ggactacgcc ttttcgaatc
     1381 ctcaaggctc tgaaagtaat gttattcttg caaaggccga taaagacact caactgattg
     1441 ggtttatcgt gcgcgttgat gccattcagc cttcctttat tcctgatttt aaaggccctg
     1501 ctcaagcaaa aatcatagag gcttataaaa cggatcttca aaaacaagaa gcgtcgaaaa
     1561 tcgcacgcga cattgctaaa aatgcaacat ctgtggagga actcaaaaag caagcagcgg
     1621 ctcacaaagt atctttttca acacttcctc cgataagcag ggcttcggtg gatcaggatg
     1681 agtcgctaaa aaatgaaatg atgggcgata cgatcgagcg tatgttcacg cttcctgaag
     1741 gcgaagctct tgcagcacct acaaggaacg ggttcaaagt cattatgttt caaagcaagc
     1801 tgccttatac cattgataat gcgaaacttg aaaaattcaa agcctcaata aaaattatgc
     1861 tgcagcggga ttttgtgcac ctttatcgca actatcttcg tcaaacacac tatcctgtga
     1921 ccgtgaatca agatcaaatg gatcaggttg tagggcacac atcttagaga ataagagggg
     1981 agaatttttc gctcatctcc cccgtttttc tagtttttgt taaaaataat atccgagtct
     2041 tctttttctg taatattgca gagttttata tagtggttta aaagttttcg atactcattc
     2101 tcgtccttag ccgcccacat tttgctattc ataccctcga cagaattttt attatagttg
     2161 agtgt
//
LOCUS       DAIAHG010000167         2114 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_167_length_2114_cov_3.877761, whole genome shotgun sequence.
ACCESSION   DAIAHG010000167 DAIAHG010000000
VERSION     DAIAHG010000167.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167_length_2114_cov_3.877761"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          584 a          435 c          407 g          688 t
ORIGIN      
        1 ttccccaaaa agatcttgtt caaccttgag ctggtttttg agataataaa gggggccttc
       61 accctcactc cagcggtctg tatgagagag aataatgcct tccttaatca gattatcctg
      121 ctcttgaacg gcagcaataa caccatcgac attgggtgtc gaaactttaa tggttgattt
      181 cagaatatag cgggaatttt caggaagctt ttgcacatcc gcatgccccc attcccgtgc
      241 aaattggtca gtaatttggg ggcccttaac ttgaatttca tcatccttaa agccgcgttg
      301 cttcaagaat gcaacaatgg catctctatc cttcacgtac ttctcttgaa gaattttaat
      361 gtcatttcct gtattttgaa agctaagatt ccaaatggcg tagtcagctt tttcttcctt
      421 ttcaacagcg acattgacat tgatgaagcg gtcatttgtg gctggcgatt ttaaggattc
      481 agcaataaaa tagcctgcaa gagctggccc cgccataatc aaaaggccaa ggacaagtgt
      541 gcgtacacag cattttttct ttttacaaca acaatgtcct gcagggcact tgcattcagg
      601 gcacatcccc tttgcgcatg tacactctga catggcgcat ccgcactctt tgcatttttt
      661 ctccataaaa taactcctga tttaatcgta cttcactagt agagtagatc aaaaaagtta
      721 atttttcgtt aagtttttat ttttttcctc ttcttctttt ttctttctca aaaattcaat
      781 gcgataagga tgctttgcga ggctttttat gaaaagttcc ccgattcttt tcatcttttc
      841 cttgtttctc tcaagctgct cctccttgga aagctggtta tagggggttt caggtgtttt
      901 ttcgtgcagg taatcaaagg ccttaagcca ctcctgtgag gttttatttt gagtcgtttt
      961 tgtttgtttg gtgattttat taggtttaaa atttttcttc ttaagaggag agtacttccc
     1021 tggtgtaggg gggcactcag gctttttctt ttcctcatca ggcgtgtgat agccaggatc
     1081 gtcgttgaga acctctgaag ccatacatgg ggctgcgagg aggagcataa gcgtatagaa
     1141 aattttcata aagactctct tttactataa gttaatggat tagactacag atagtaaaag
     1201 ttagtattca ttcaatcaaa tatttttatt ttccttaatt aatatgatta ttatattaaa
     1261 ttatttcatt aattctttta aatttttaaa aaactccaaa ctttcgggat ttaaaaaagc
     1321 ctcttcatta tcaatcgcat gaccaaggaa aatactttta accgctattt ctgcaatttt
     1381 attatttttt gtccgtggaa tatccggcac ctgaataatt ttagcaggaa cgtgacgagg
     1441 tgttgtttgg gtgcgaatgt ggtgttttat ttgatcaata cgctcgtctg taagtgtcag
     1501 accttctttg agcctaagga ataaaagaat acgctcatcc ccctcaaaag gctgccccac
     1561 agctaatgct tcgacaacat catcgagtgt ttcaacctga cgataaattt ctgctgtacc
     1621 aatccgcaca cctccaggat taagaatagc atctgaacgt ccactaatga taaaaccgcc
     1681 gtgctctgtc ttttcaatat aatcaccatg gtgccaagca tgttcaaagc gcgcaaagta
     1741 cgtattatga tagcgcgtac cttttgagtc attccaaaaa caaagagggc gacttgggaa
     1801 agcattgcga cagacaagct ctccttgttc gccgacaacg gcaggtgttc catcaacttt
     1861 aaagacatcc acatcaaggc caagaccagc tgtttgaatt tctccacggt aaacggggct
     1921 tatgggattg cccaatacaa agcaagaaat aatatctgtt cccccagaaa ttgatgcaag
     1981 gcatagatct tttttaacaa agctatagac atagtcgaaa gtagacggca tcaaaggtga
     2041 tcctgttgat gtgatcattc gcagcgatgg aagagtatat ttttccttta tattgatatg
     2101 atttttagaa aggc
//
LOCUS       DAIAHG010000168         2109 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_168_length_2109_cov_3.677165, whole genome shotgun sequence.
ACCESSION   DAIAHG010000168 DAIAHG010000000
VERSION     DAIAHG010000168.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168_length_2109_cov_3.677165"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          593 a          450 c          475 g          591 t
ORIGIN      
        1 tattgaagtg tggaataaaa tggatctttt gacctccgag gccaggactg tgtatcagaa
       61 taaacaagta cttcaaaaaa atagtatttg cgtttcagct cttcagggcg atggtctcga
      121 tgatcttaaa aagcgtatcg atgtgaagct tgccgaaagc cgtaaagtta ttcacgcaac
      181 attgcagtct tctgcgggtg agcttttagc ctggtgttac acaaaaggtg caattagcga
      241 gcgcgtggat gacggcgagc atattcacct gacgctgcgt ctcccaaaag cccttgtccc
      301 tcaattttgg aagcgctacc gcaaggaaat tcaataaaaa aaggacatat tatgttgatc
      361 aaaaaaataa gaaaagtact gttatttttc accctcgttg ttttactcct cagcctttat
      421 atcttcaggg aaaatattct ttttcaacta ctcacaaaaa tagatttatc ccatcttgat
      481 gttcagacag cgcgcacctt agcaacacct attcttgatg ctgcatcaat tccggacggt
      541 gatttatcgc ttacaagcca cacggctcat agcaaaagat atattatctc aaaatggcaa
      601 agggccgctg ttggggcaag tgtcattgtc tttgggcgac acccagatgg gacattatcg
      661 gttattcttg gcccccaaag aggaatgctc cgaaatcctc aagggtatat ggaaataccc
      721 cttcccaaag aggatttaac aggattaaaa gcaagccata gcagccgttt gaatggcaaa
      781 aaactcaaaa aagtgaaaga ggatctttcc ctcgaagata atgctgtgag agaagttttt
      841 gaggaagcgg gtctgaccat taaaaaagaa gatctcgtac tccttaggat caatagcgaa
      901 atcaacgcaa accccatatg ttttgcagct caatatatgg ttattttgaa cgatacgcct
      961 aagctctcaa accatgcgag tgagtttgtc aacgatgact tacaaaagcc tacctgggtg
     1021 aagctttcgg atattcatta taatgctcgt gacaagcact actttgtcaa agattatccc
     1081 atgcctattg atccgaaaac catcgattcg atgaatcttg ggcttttaaa gatgggactg
     1141 aatccccttg cccaaaatag ctctgaaaga gggtgaaatt tgaagaaaag caccgtatag
     1201 tacgcgtaaa tgacttaagt taataagaca atgaaaatac ttttttgcgg tgatgttgtt
     1261 gggcgctcgg gacgtgatgc cgtgttggat catttgccac gcctccgccg tgatcttaat
     1321 gtggatatta tcattcttaa tgccgagaac gctgcccacg gttttggcct tacgcgcggc
     1381 attgcagagg atttttataa agtcggcgtg gatgttatca cccttggcaa tcatgctttt
     1441 gataataaag aaattttgcg cagcattgat acagatgcac gccttgtacg gccacttaat
     1501 tatcctctca aaacgcctgg gcgtggattt acatttattg acctaccgaa tggcaaaaaa
     1561 ttacttgtga cgaatgtcat ggcgaggctt tttatggatc ctttggatga tccttttgcc
     1621 gcggtggatg cgcttcttgc caaatacccc atgggtggga gcatttatgg tatttttgtt
     1681 gattttcacg gcgaggcaag cagtgaaaaa atggcgatgg ggcactattt ggatggaaaa
     1741 atcagtgctc tcgtcggcac gcacactcat attcccacag cagacgcaca gatccttcca
     1801 aagggaacag gctatcaaac ggacgccggc atgtgcggtg attataattc tgtgattggc
     1861 atggataagg ctgttccgat tcagcgcttt cgcaccaaaa tgcccacaga gcgtatgcag
     1921 cccgcaggtg gccccggtac tttttgtggc acgttcatag aacttgatga gcgcggtctt
     1981 tgcacccata ttgagcccgt gcgcgttgga ccgcggttga tgaatacaca ttagaaaagg
     2041 attattttct tatgaaaaaa ctgtgccttc gccttacctt tttaatattt tttgctatac
     2101 cctcggttt
//
LOCUS       DAIAHG010000169         2109 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_169_length_2109_cov_3.157972, whole genome shotgun sequence.
ACCESSION   DAIAHG010000169 DAIAHG010000000
VERSION     DAIAHG010000169.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169_length_2109_cov_3.157972"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          602 a          376 c          461 g          670 t
ORIGIN      
        1 ggatcaaaag tttttcgcga tcattcctag aaaaatcttg acagaatacc ccttttcaag
       61 atagtaaaaa gtaaacacaa ttatgttttt tcatatttta gaaagatatc agacaatgtt
      121 tgcagtgatt aagaccggtg gtaagcaata ccgtgtccaa gaaaaaagca tccttcgcat
      181 tgaaaagctt cctgttgagg cgggtcaatc tgttacattt aacgacgttc ttatgatctc
      241 cagcaaggga aaagtgtctg taggtacgcc gatggttgca ggtgcagttg ttgaaggtaa
      301 ggtgattgaa caaatgcgtg atcgtaaggt gattgttttt aagaaaaaac gtcgtcaaaa
      361 ttatcgtcgc aaaaaaggtc atcgtcagca tctgactgtt gttcaaattt caaaaatcag
      421 ccataaagcg taagcttctg gcactgttaa ggagtatata aaatggcaac aaaaaaagca
      481 ggtggtagtt cgaagaacgg acgtgactct gagagtaaac gccttggtgt aaaagtttat
      541 ggtggacagt ttgctctcgc tggcaatatt attgttcgtc agcgcggaac aaagtttcac
      601 cctggaaaga atgttgggat gggacgtgat cacacattat ttgctatgag tgatggtatt
      661 gttcagttcg gtcgcggcaa agctaataaa gcttttgttt gtgttgagcc catcgtttaa
      721 gtttttttag actcgtattc taaagtaaaa aaccccctaa attttagggg tttttttatc
      781 aattatgttt cgcgctagtt tttctttttc ttaccccttg gcattgttgt tttcacagca
      841 tttttcataa tcatcttaag ctcttctttt ttgagttttt tggcatatat ttttagatct
      901 tcacctttgt ctttctcatt actctcatag tttgcaacaa cggagagatt cggattaata
      961 tcattgggac agaaatctgt cacggtttct tttgttccct tatagggttt tcctcggtag
     1021 ccactctcaa cacattgggt atgaaggcat tgtggttttc ccgtttcgta attgataagt
     1081 cctcgttgga cagagcttgg gccatagcgt gtttcattcc tatcttcaaa ttcgtagcga
     1141 tattggtaaa agcctgcgtg tgttctgtat ttgtcgcaat tcaactggag ataaataaag
     1201 gggcgaggtt cttcaacgtc tacatcaatt tcataaaaag gagttttatc actcttaggg
     1261 cagtatcttt cgaaattttg gttatgacca ttaaaattta aaatgctgct aaggcaattt
     1321 gatgtatccg taggcgtgag gttattattg tttttaacgg tgtatccgag gtttgttgca
     1381 ccaaagacag cggctgttag aatgcctcca aaagcaagag ttttgtaaac agtatctgag
     1441 gatggaatgt agtattgaac tggttcgggg attatatccc aaattttttt aaatgtttga
     1501 ctatagaatg tttggcgcgg ggggggggga gtcattatta agggaattat attcctcttg
     1561 gtttgatgcg atggcttgtg tgaatgtgag ccctgaaaaa aggctgagtg taatgagtgt
     1621 gcgtcgcatg ctgttcatta aaattatcct aaaattttat taataattcc atgctaaagg
     1681 acatatcctt taaggcgcaa gcgcaaaata ttttctttta aggaccgtta tcgtagcttg
     1741 agtgtggaga gtccaataat cgccaggaca cttgcgataa tccaaaaacg aatcacaatg
     1801 gtaggttcgg cccatccttt tttctcaaaa tgatgatgaa tgggcgccat gagaaaaatg
     1861 cgttttttcg tcattttata atagcctact tggataatga cagaggcggc ttcgatcaca
     1921 aaaagccccc caataatcgc taaaacaagc tcgtgttttg taatcattgc aatggttcca
     1981 agggcacctc ccgcggcaag agagcctgta tcccccataa aaaccttcgc aggcggtgca
     2041 ttaaaccata aaaagccaag gcctgcaccg ataaaagcac cacaaaaaat agcaagttct
     2101 cccgtatga
//
LOCUS       DAIAHG010000170         2082 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_170_length_2082_cov_2.323192, whole genome shotgun sequence.
ACCESSION   DAIAHG010000170 DAIAHG010000000
VERSION     DAIAHG010000170.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170_length_2082_cov_2.323192"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          592 a          439 c          451 g          600 t
ORIGIN      
        1 aacgattatt accgcatccg aagaggatat tcatgtgtct ggtcaccctc atcgtgatga
       61 gcttaggcaa atgtatgaat gggtgcgccc tcaaacactc attcctgtgc atggtgaggc
      121 acgtcatctt tatgagcatc gtcgctttgg tctttcctgt ggtgttcctc acagtattat
      181 tccgaataac ggcacgctga ttcgtctatc gccaagccct gcagaaatca ttgatgaaac
      241 aattcctgtg gggtatagcg tgaaggatgg ctcccgtgtg attgcacaag aaagccttct
      301 cttgaaacaa cggggtaaac ttgcgattaa cggtgcactt tttgtgagtg cagctgtgaa
      361 aaataaaaag attatcggcg acccacattt tagcattcat ggattaagtg aaccgggaga
      421 ggaaacagga ctcttgatgc ttgagcttgc gcgtcttcta aaaaatacac ttgaggagag
      481 ttttaagagc gacaaagaac gtcatgagga aatttttaaa aatctccgcc gtcactgcat
      541 gctgcgcttt ggcaaaaagc ccgtcacaga gattcatatt ctggatgttt aacaaacaga
      601 gggttttaag atcagtgaat ctctatcact taggccgcat ctgtttttct taaaaaaata
      661 aggggatctg caaggataaa gcgtggggtg agatcaccaa agccaatctg tttgctctca
      721 tcatgactgg accgcccacg gcccctttta tcggattctt ttgcatgaaa atctttggag
      781 gtttcttctt taacatcatc gagagtaaga gcataatcct gaggctccat cccgatagtc
      841 tcaataatag ctttcaaaag ccttgcatca cgttttgaga caaagctaaa tgccacaccc
      901 ttttttcctg cacggcctgt gcggccaata cggtgaacat aatcatccgc atgaatgggg
      961 agatcataat taatcaccaa aggtaagtca tcgatatcaa tgcctcgcgc ggcaacatcg
     1021 cttgcaacaa ggataagccc tttttttgta cgaaacgtat cgagggtctc gttgcgtttt
     1081 gattgaacca agtcgccatg aatgggcatg cttttaaatc cagcggattt cagagattta
     1141 gcgacaatat ccacatcctt ttttttgtta caaaacacga tggcagacat gttttcatag
     1201 agacgtatca atgtgcgtag tgcctgcagc ttgtcttctt ctttgagatt aattttaaat
     1261 tgctcaatag tagacgccgt tttcgtttct tcttttgatt cgatatactt aggctctttt
     1321 aaaagagagt gaatcaaagc ctcgagctcc tttggaaagg ttgcactgag aaggataatt
     1381 tgtttttcag agggtaaaga gttgagaaga cgtgtgacat cgggcataaa tcccatatcg
     1441 agcatgcgat cagcctcgtc aataacgagt gttttaatgt cgagcatcaa aatttttcct
     1501 cgctcataca gatcgagaag gcgtcctggc gttgctataa taaggtctgg ttttttacgc
     1561 agttgttttt cttgatcacc tgtccactca cccccgacta aaacggcagc ttttaagcct
     1621 aagccctttg agaaaagttt aaaattatca tgcacttggc ttgctaattc ccgcgttgga
     1681 cttaaaataa tcacacgcgg catacctgca cgcatacggg tatgaagaag tttgtcaata
     1741 ataggcagca aaaaagcacc cgtcttgccc gagcctgttt gagcgcatcc acacacatca
     1801 tggccttgaa ggataagtgg aatggctgct ttttggatgg ccgtaggtgt ttcgaaccca
     1861 agaggcgcta aattagcaag aagctccttg gagagagcaa cgtcagaaaa agaaaatgtc
     1921 atggagttat aagtttttat atcctgtctc taccattggg gattaaaatg ataaattcaa
     1981 gcaaaaactg agtattaagc gcgattttgt aggataatgt ttattttttt attcttccta
     2041 tccgtataga aaatctcaac aaaaaacaca tacatacccc ca
//
LOCUS       DAIAHG010000171         2007 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_171_length_2007_cov_2.835233, whole genome shotgun sequence.
ACCESSION   DAIAHG010000171 DAIAHG010000000
VERSION     DAIAHG010000171.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171_length_2007_cov_2.835233"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          584 a          444 c          423 g          556 t
ORIGIN      
        1 ctttttaata catctattta ttctaaatat ctatattttt ttcataaaat attaatatat
       61 atgtgttttt atagtaacag attaaaatat tatcaataat ctcttgtgat catcgacaaa
      121 aatcatctga gtaagctggc ggatatggcg tttattcgcc aactcatgaa agagcctctt
      181 ttagaaaaag cggaggaaac gagccttaca cttgcgtgga aaaatcacca tgatgaggaa
      241 gccttgcacc agttaatcaa gtccttctcg cgccttgttg tcagcatggc ccttcgtttt
      301 cgtttctatg ggcttcccct cacagatctt attcaagagg gcgtcgttgg gcttctctat
      361 gccgcagaac gctttgatcc cacgcgggag gtccgttttt ctacttacgc aaagtggtgg
      421 attcgcgcca cgattcaaga ttatgttctg cgtaattggt ccattgtccg aacaggctct
      481 acaacggctc aaaagcaact tttttttaac cttaagcgtc tcaaaaatca acttttagac
      541 ttttcctctt caagcaccct tgacaaagaa ggaagagagt ctatcgccca cacactccat
      601 gtcaatttgc gcgatgttga agagatggaa aagaggctat ctctccagga tttatcgctc
      661 aacacttata ttcacaatga tacgacacaa atatggcaag aatttttaga agacgagcga
      721 cccggtgctg acacaaccat tgaaaatgcg gatgatgcgc tttggcgtca tcattggctt
      781 catatcgcaa tggaacagct caatgagcgc gagcaatttg tcatttatgc aaggcgcctt
      841 caagatgatc cacaaacact agaagaactc ggcaggtctt tgagtattag caaagagcgt
      901 gtacgccaga tcgaagcccg cgccctccat aaaatgcgcc acgtccttat tacgcatatt
      961 cacgatattc gcgatgtttt ttaacatttg gtgtttaaaa aaaattgacg cagccacaaa
     1021 atagctgtat atataagtca ttcttaaagg ggcccttagc tcagtcggta gagcatctga
     1081 cttttaatca gggggtcgaa ggttcgaatc cttcagggcc caccatcttc tcacaatcaa
     1141 agattttaaa aaatacgcca tggctgtggc attgctcata accattgcgc aatcttctca
     1201 aaacgataga aaaaatatca agcctaggca gaaataatca cccttttttg ataaaactgt
     1261 ttcgaagaca ttataaatcc tgtaattcta gaacaaacaa gaagactgaa tagactggga
     1321 tttttatggg atttaactgt ggtatcgtgg gccttcctaa tgtgggaaag tccactcttt
     1381 ttaacgcact aacggccaca caagctgccg aagctgcaaa ctatccattt tgcactattg
     1441 accccaatac agggcgcgtc ggtgtacctg accctcgtct ctatcttctt gcagagcttg
     1501 caaagtctca aaaaattatt ccaacacaat tagaatttgt ggatattgca ggccttgtac
     1561 ggggtgcgag caaaggcgag ggcctcggca atcaatttct tgggcacatt cgccaggtgg
     1621 atgctatcat ccacgtttta cggtgctttg aggatgatga tattacccac gttgatggaa
     1681 aagttgaccc ccttcgcgat ctcacaacca ttgaaacgga attaatgctg tcggatatgg
     1741 aaagccttga aaagcgtgtg gcttcccttg gtaaacgcgc aaagggcggc gataaagaag
     1801 ccaaagaaat gctggagatt gcagaagatg ttttaaaatt gcttcaagat ggcaaacctg
     1861 caagccttta cacgcctgat aaaaataaag acatccttta tcaacaactg catcttttgt
     1921 cgtctaagcc gattttatac gtgtgcaatg tgtcagaggg ggaggccgcg acgggtaatg
     1981 cctacagcca ggccgtggcg gactatg
//
LOCUS       DAIAHG010000172         1996 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_172_length_1996_cov_3.263679, whole genome shotgun sequence.
ACCESSION   DAIAHG010000172 DAIAHG010000000
VERSION     DAIAHG010000172.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172_length_1996_cov_3.263679"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          542 a          400 c          479 g          575 t
ORIGIN      
        1 ttaaaaaatg catcctctac agaatttggt ccaacggata cacccgttat cgggctaatg
       61 acagaatgga tgagtaaaga tacgcttcaa aagcgccatg ctcatcaaga tttgggcgga
      121 acaatgcgtc ttggcgcgta tccgtgtcat attcatccac attcgcttgc tcatgcaatt
      181 tacagagagg ctgtgatttc agagcgccac cgccatcgtt acgaggtcaa tacaaattat
      241 aaagatatta tcgaagcgac cggccttgtc ttttcaggga tgtctgccga tggaaagttg
      301 ccagaaattg tcgagcgccc tgatcatccg ttttttatcg caacgcagtt tcaccccgag
      361 cttaagtcac ggcccacagc cccccatcca ctttttaaag cttttgtaaa agcagccctg
      421 gaccggcggc agggataatg attgttagtg tggagaagag ttgcgcctcc tcctttttct
      481 tagttattga tagcaggaag aatttttcca aaaagatttt gaagtctctc ttctgctttt
      541 ttattaaggt tagtcttact taagtgctga ggagttaggg gatctaaacg cgcttcgtta
      601 actcgtattt cgttgtctcg tgctagtgtg agggaccctt cgttttcatc tctctcccaa
      661 cccttttgag gcatagcgat acggagcatg agacatgcga ttattttttg catcgtaaaa
      721 tctccaccct taagaggtgt gagaggggaa ttatcttctc gtctcttagg cgagtgtgct
      781 aaatgttaat aaaagattaa aatttaaggt gttcaatgca aaagcatgtg cgtgtaggaa
      841 atgtaacgtt tggaaatgat ctgccctttg ttttggtggc agggccgtgt gtgattgaaa
      901 gccgcgagca tgccatgatg atggctgaga aacttaaaaa tcttacaggc aaagccaaga
      961 ttccttttat ttataaatcc tcctatgaca aagcgaaccg attgagtgtt aaaagcgagc
     1021 ggggcgtggg tcttgatgag ggtcttcgga ttttagaaga ggttaaaaaa accttcgatg
     1081 tgcccgtttt aacggatgtg catgataacg agcaatgcgc ggcagcagcg gacgttgtgg
     1141 atattttgca aactcctgcg cttttgtgtc gtcagacaga ttttttaaaa gcagccgcaa
     1201 aaacaggaaa gcctctgaac attaaaaaag gacaatttct tgcaccgtgg gacatgaaga
     1261 atgttgcgaa gaaagtagag tcctttggca atcataatat tattttgtgc gagcggggtg
     1321 tcagctttgg ttataatcaa cttgtgtcgg attttcgtgg ccttgatgtg atgaaagaaa
     1381 caaagcaccc cgtgatgttt gatgcaactc attcggtgca acaaccgggt ggtcttgggg
     1441 atgcaacggc gggagaacgc aaatatgcgc ctcttttgat gcgtgcagcc attgctactg
     1501 gtgtggcgtc aatctttatg gaggtgcatc aagaccctgc tcatgcgcca agtgacggtc
     1561 cgaatatggt gcctttggac tatgtgcctt atcttttgcc ctatatgcgt gaccttgatg
     1621 agaggacaaa aacatattta gcgacacttc cgaccatgga agaaatttat caaggctaga
     1681 tttttaatcg caatattttt tgctgtgcac gcttcattta tcatgaaagg atcgtatgaa
     1741 attactcacc catacaaccg caaaatcctc tcactataat ggagaagcgc agtattatga
     1801 cgactttaat gaaaaaaatt ccgaaatcac caatgctgtt cttgaaaaaa tattaaaaaa
     1861 atacgaggtc tcgacagtgc ttgatctgac atgcggtaca ggctctcaag tcttttggct
     1921 tttggatgca ggttttgacg ttgtgggggc ggatatcaat gcaagcatgc tgcgtgttgc
     1981 atggcaaaaa gcaatg
//
LOCUS       DAIAHG010000173         1985 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_173_length_1985_cov_3.553459, whole genome shotgun sequence.
ACCESSION   DAIAHG010000173 DAIAHG010000000
VERSION     DAIAHG010000173.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173_length_1985_cov_3.553459"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          487 a          446 c          351 g          701 t
ORIGIN      
        1 tgcctgaaac aaaaaacaca gcggacgagc ttcgcatcct cccctcgcaa gttctttttc
       61 ttgaggcgca aattgatcat tttaatgaaa aactgcagcc cctgcgttct tttgttctgc
      121 ctggcggatc acatcttgcc gcaacattgc atcaatcgcg cacgattaca cgtcgatctg
      181 aacgtgtagc ctgtcacctt ttggaagaag attctgcgct gaatctccat attatcaagt
      241 atcttaatcg cctctcagac cttttgtttg tcctcgcacg cattgccaat gataacggaa
      301 aaacggatgt tttgtgggtg ccgggcgctc atcgttaggg gcgtattagc atcaaaatac
      361 aacaaccttt tttagtaaat aaacatatat tatgtacatt tactacatgc aacattacta
      421 tatgcagttt tacactatac gtactgacta aaaaaagcac gcaatggctc actcacatga
      481 gaactttctt ttctatcttt tttcttatcc ttgcatgttc attaaatttt ttttaaaatt
      541 atttcataaa gattttatct aaaacacatg atgaaccttc gtcttggcac tgattttatt
      601 gcaagcatga tcacagggag catcattggc tattggattg atcgctggtt tgcaacctcg
      661 ccctttttcc ttattctctt tattatcttg ggctttgccg caggatgtcg tacactgatg
      721 agagcctctt ctgctcagca aaaatcctcg ccttccaaag gagacgcacc cgatgcatga
      781 tcctcttcat cagtttgaag ttcaccccct catcaagctt gagcttatgg gctacgatat
      841 ctcctttaca aacgcatccc tctttatggt cattgcaacc gtactcgtct gtcttttttt
      901 tctacggcaa aagcaagagc ttgttcccgg atatatccaa tatattcaag aggccagtct
      961 cacttttatt aataacatca cgcacacgtc cgtaggcccg gaaggtttgg tttttgcacc
     1021 gtatattttt tgtctatttt tatttatttt tgcaggaaat tatattggcc ttgcgcctta
     1081 tacatttaca ttcacaagcc atcttattgt aacatttagc cttgccatta tcgtttttct
     1141 aacgacaaca gtaattggat tttacaagca tggttttcat tttttacgct ttttttttcc
     1201 aaaaggactt cctcccattg tcgcccctat cctgattccc attgagatta tttcctatct
     1261 atcgcgtcct gtcagcctca gtattcgtct ttttgcgaat atggttgccg gtcacacgat
     1321 gcttaaaatc ttcgctggat tcgccctcgt tcttggttcc acggagtttt tcccagcagc
     1381 gcttttaccc attattgcga atgctggagt gcttgtcttt gagtgtttaa ttgctttttt
     1441 gcaagcttat gtttttacca tgttaacgtg catttattta aatgatacgc tgaatttaca
     1501 ttaatttttt ttaaggagtt tttttattat ggatattcaa gcagcaaaaa tgattggcgc
     1561 tggccttgca atgatcgcac ttcttggtgt tggtcttggt ttggggaatc ttttctcaaa
     1621 tcttattaat gcaattgccc gcaatccttc cgccaaaaat gagattttcc ctgtgggcat
     1681 gattgggttt gccttaacag aatcaattgc tcttttagcc tttatcgtcg cgatgattat
     1741 tctctttaaa tagatgtgac gttttatgcc tcagtttgac ccgactcatt ttgtctcgca
     1801 gattttttgg atgcttattt gcttttttat tttagtcatc gtcatgacac agtttgctgt
     1861 gccccgcatt caaaaaggca tccataaacg cgcaaattac cttcaagatc ttaaaaacct
     1921 tgaggggatt tacctgacgc aaattgatga actgacagag cgcatgaata cgctgaaaca
     1981 agaac
//
LOCUS       DAIAHG010000174         1959 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_174_length_1959_cov_3.688098, whole genome shotgun sequence.
ACCESSION   DAIAHG010000174 DAIAHG010000000
VERSION     DAIAHG010000174.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174_length_1959_cov_3.688098"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          553 a          391 c          410 g          605 t
ORIGIN      
        1 taaaggataa taatatttta aaggatagcg aagaatttca tcatatcctt aatgtaggtg
       61 cttttacgag tggcatgatt aatacaattc tatattcctc tgacagtgag ttacaagagt
      121 atcgagaagt gtttaaaaaa cttattttta aaaactgccc cataaacggc atttcaattg
      181 gtattcctaa ggggtataca ttcaaagagg cagctaacga tcttgtaaag atcatatatc
      241 aaaagcttaa tgaaagcagc gattcaaact aatatcggaa gaagcttccc tcgctttggt
      301 gtgggggagt actccttaaa tagtcgttcc ctgatgaaaa atcatgcgcc acttgttttc
      361 atcccctttc cacagcgaag aacgcaacga tgatccattt tcttccatga ggacataggt
      421 catcagtgca acatcctttg atattttttg cacctcaaaa gcatcgaccg tgaattttcg
      481 atctttttct aaaggaagaa gcgcaaggag atcttgtttt gtatacatca cgcctgagct
      541 gccaaattca cgaaaagaat cgtcaataag ttgttctaaa agagccggtg atgcccttac
      601 tttcacatcc aaaagagaaa gctcttgctg ttcaagatgt tctgccagcg gggattttcg
      661 aagcatccat actgtgtcaa catccgtatg cggagcgatt tctttaaaac ctgctttctg
      721 ataaaggcga atagccgctc tattttttgg atctggatcg gcaaacgtat aggcataatc
      781 ttcatcaagg attttaaaaa acaactccaa agccttagac gccactgtgc gttttaaaaa
      841 atcgctctca ccgatgaaca catcaaacgc gctaagatta ttctttaacc ccatcagagg
      901 gtttgaccgt ggaaaatcat gcgcatcata gccctgaaca taccccacag gaacatcatc
      961 gacacagatc acaaaagcgc gcatgcgttt ggcaatgcct ccctcgattt tataaccttt
     1021 gacataatca ctatatttcg cgtggatcag cgacggcgtc cacgttacat gaggatccca
     1081 aaattttttc acatggggtg tttcaagcca cttcaaaagc aaaggaaaat gggttgtatc
     1141 aaggggagca aggctaattt tcatacgcta aaatctgctg taacaggaaa atttttcatg
     1201 gatgctgttg atcatgggga aaattttaat cactatagtc tagaataata attattttta
     1261 ataaaatttt tgagtctacg atgttcgatc cttacgtcag aaaaatgatc gacccaccgc
     1321 tgaatgtcat aggaaagcgt ttagcagaca ttggtgtctc tgccaatagc ctcacgcttt
     1381 tggggttttt tctgggtagc tttgccgttg tggctattat gcttgggaga tatagtgtgg
     1441 ctgcgctttt tattgcgctt aatcgtcttg cggatggttt agatggtgcc gtggccaggc
     1501 atactcattt gacggatttt ggaggttttc ttgatattgt ttgcgatttc ataatttatg
     1561 cgggcgtcat ttttgccttt ggggtggtga ctacagaaaa tttgttttac gctgcctttc
     1621 ttcttctaag ttttattggg cccatgacct cctttttagc ttacgccatt atcgcaagtc
     1681 aaagaaaaat agtttgtgaa cggcgtggca aaaaatcttt ttactacctc ggagggatct
     1741 gtgagggcac agaaacggca tgtgtgctgc tttttatatg catgttccct gcgtcttttc
     1801 aaactgtatg tattattttt ggcatcttat gctggctcac aacccttggg cgtatgtata
     1861 acgcctgggc tgattttgct taaaaaattc acctaaggtg cgtttttccg tcggacgata
     1921 acgtgataaa taacaggtgc aagggataag aaaccaagg
//
LOCUS       DAIAHG010000175         1948 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_175_length_1948_cov_3.449492, whole genome shotgun sequence.
ACCESSION   DAIAHG010000175 DAIAHG010000000
VERSION     DAIAHG010000175.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175_length_1948_cov_3.449492"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          611 a          358 c          362 g          617 t
ORIGIN      
        1 gcttttggcg tcactcagat agtgatgtaa tctttctaaa tttgcttcag tcacttcttt
       61 aagtatctgt tgtgcaaagt taatatttgt gcgcttattc ccatcgtaca tataggaaag
      121 aatgaaaaag aatgagagaa ggaaaagaga aagaaaactt aagataatat caaaacgcaa
      181 gcgcctgtag tgaataaaat tcaccatttt tttagtcata gcctcttttg tgcgcttaaa
      241 aatatccata tttttttcaa agaaagaaca taaatttttg agtcagtctc ataggcactg
      301 cctacttacg agtttatttt aaagggtaaa aacttaaaaa aagcttaatt ttttagaaaa
      361 atagaacctc tctttttaat caaaaagatt tttttgttta cggtttgtta agattttttt
      421 gatctaatgg catcattaaa tgcaaaataa caaggagaac tttaatgata aaatacattt
      481 ttttatttat ttttattaac tctaaaatat ttttatattc agctggtgct tcttctgaag
      541 agaaaggttt tcaggaagaa aatgggccgt tgattaaaaa aattttaaaa aataaatcaa
      601 aagaaaatct aaaagaagaa gaaaaatcgt tcattatttc attggataca aagaaagttc
      661 cagaacaagc ggacgaggaa ttttatgacg cggttcttta tggttcagat gatgagaaag
      721 aggaagtcat cacccccttg cattcgcctg gaatcacgcc caaaaattcc tcaaaggata
      781 taaaaagaga aaattccctc aaaaatctta aaaatgacaa tgatataaaa tatatgaact
      841 aatggcttta gatctcagac gacctgactc ataaaacctt ctttttcggg tttttttgag
      901 gcgtgaagtg ccggcaccac atcctcgaca cttgcaacga cggagaaaat ggtccggtgc
      961 tcaggcaatg caaacttcat atcgaccacg tgatacatca actctacaag ttttgaccaa
     1021 taattatagg cattcacaat aagaataggt ttgtcgtgaa gcatcagctg cttccacgtg
     1081 ataatctcaa aaagctcatc gagtgtgcca aaccctccgg ggagcacaat aaaagcatcg
     1141 gcgcgctcgg acattttgag tttacgtgta tgcatcgtat ccacaaggtg gagttcttgg
     1201 atacctgtat gcgcgccttc aaactggtta agatgatgcg gcataaaccc cacagcacgg
     1261 cccccattat tgacaactgc atctgcaatg atgcccataa gccccagttt tccaccccca
     1321 taaacaagct caagcttcgc actcgcaagg gcaacaccta catcatgcgc aactgtttcg
     1381 tagagttcat cgccattatt atggccgcaa tagacacata cactttttat atttttcata
     1441 atcatacgct catatttcct aaaaactcat ttttatcata acagttaatt gtttaaagat
     1501 ttgttaaaaa ttcatggttt tttgctttat ttcttgaatt ttttcgatga tcaaggcagt
     1561 ctagaggcta gtactcgatg ttttttttaa agggagctcc ttttgcgtat tcatccagat
     1621 ggttggaaat ttgttggtct ttttgccgca gtcacactta tttttttcct ttttagtaca
     1681 gcccttgggt gggtcggtat tatcctaacc gcatggtgca tttatttctt tcgcaatcca
     1741 gaacggatga cgccaacacg tgaggggctt attgtcagtc ctgcagatgg caaagtcgta
     1801 ggggtaaagt gcgtcgttcc accaaaagat tttgatatgg gggatgaggc ccgtgtgcgt
     1861 attagtattt ttttgaatgt ttttgatgtg catgtaaacc gtcttcctgt ggggggtatc
     1921 attaaaaaaa ttatttatca tccaggta
//
LOCUS       DAIAHG010000176         1947 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_176_length_1947_cov_3.113904, whole genome shotgun sequence.
ACCESSION   DAIAHG010000176 DAIAHG010000000
VERSION     DAIAHG010000176.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176_length_1947_cov_3.113904"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          547 a          332 c          469 g          599 t
ORIGIN      
        1 ctataaggaa aaagttaata ttttgtttat ttctcccgtg ataaagtaag aaataaaatt
       61 ttttttaaga aagaagtata ccttgttaaa gtgcctttta attctgtgtg tcctagggtt
      121 tggatctctt tatggagctg tgtttaatcc cgatatggcc gctattacag caaaaattaa
      181 agagtatgct gaaaaatacc gaggcgtttt taatacgccc gggctgagta ttattattgt
      241 gaaggatggc aagagttcta ttattaattt aggtacattg agtgtcaatg acgcgacacc
      301 cgtgaacgat catacgctgt ttcaagtcgc gtctgtcaca aagaacttta cagcaacgct
      361 tatggcgcgc cttgttgaaa aaggtgtgct ttcttttgat gacaaggtga gtaaatatct
      421 ccctgatttt caactttctg ataaaacggc aacacatgat ttacggatta aggatttgct
      481 gtgccattgt attggtcttg aggattttgc aggggatacg ctatggcatg gaaggcttag
      541 tcaggaagaa gttttaaagc atatgcgtga tctgcctttc aaaagtcact ttagaagcac
      601 ctatgcgtac agtaatatta tggtggggat tgcgggtctt atcattgaaa aagtcacagg
      661 aaaacccatt gatcaagtga tgaaagaaga gctttttacg cctcttggga tgacagaatc
      721 ttctgtgggc tttgaagggg ttcatacgca ggtatccctc attgatagag ctgtgaattt
      781 tttaaaaaaa acgtcctctc aaaacaaagc aaagccccac gatttgaaag atggtaagcc
      841 gaaggaattg gatttttata aggaatttta tcttttccca ggttcaagtg gtgtgaatac
      901 aacgcccgat gatatggcaa aatggttaaa ttttcatttg aacgggtatc aaacctttac
      961 gaatcctccg aagacactga tttcgccgga gattgtcaaa gtaatgcgtc aaagttatgt
     1021 ggatggttct gaggagcttg cgggtcagca gtttccaagg gagcgtgtga cgcacgtgag
     1081 ccttggcctt ggttggttta tgtatgacta tggtgtgggg caaaagcgtt tgaatgtctt
     1141 agagcaaatg gggggaatta ctggggcgcg tgcgcttttg gtgattgttc cctctgaaaa
     1201 tctgggaatt gcggtgcttg cgaatttagg aggcatgcgc gcgagtttgc ttcctgaagc
     1261 gattgttcaa aaattcttgg atctttatat ggggattgat gacacagatt gggcagcgaa
     1321 aattcatgat aaatttgtaa aaattaaaaa taatcagaaa ttgcaactcg atgccatgcg
     1381 tcttaaaaat ccagagccgt ctgcgccgct taaaacttat gaaggtcact actctaatcc
     1441 tatttatggg gatgttgatg taacgaccga tgatcaaaaa aatctttcaa tgctctaccg
     1501 tgggtataaa attcctttga aacatatcaa tggtcattat ttttattgct caggccagga
     1561 tctttccgag ggtttttctt ttgatgatcc ctgtattatt gagtttggtg tggaagggtc
     1621 tgctaaaacg tcctatgggc ttatgatttc attgctggat gaaggggata attcggcgtt
     1681 ttatcgaaag gatcaatagg cgcttttgcg tttactttaa tatgcataga acaaggctaa
     1741 gatccttgga agttaagggc atcatctgtt ttaaaggcaa tatccacgac accgtgaggg
     1801 gcaaactgag cgataaaatc cgtgtaccat gtggtgcgct ctgatccccc ttcgtccttg
     1861 aggaggagct gctgaccata aattgcaatg tcttccaaga tgcgctcatg gcgataataa
     1921 gccaaaagcg tttggttaat aggtgct
//
LOCUS       DAIAHG010000177         1916 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_177_length_1916_cov_2.709625, whole genome shotgun sequence.
ACCESSION   DAIAHG010000177 DAIAHG010000000
VERSION     DAIAHG010000177.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177_length_1916_cov_2.709625"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          619 a          316 c          387 g          594 t
ORIGIN      
        1 gccttttgat ttttagaagg caactctgtt tgttcgcaga ttgctacgtg gacatctaca
       61 tcaagaagtt ttttaacgta attcaaataa ctatgtgctg gaacaccgca cataggcaca
      121 ggttccccag cagattgtcc acgggtcgtc agtgtaatat tcagcaactc agaagccgtt
      181 tttgcatcat cataaaataa ctcataaaaa tctcccatcc tataaaaaac aagagtatcg
      241 ggatattctg atttaatttg caaatactgc tgcatcatgg gcgtatgagc atttgacgcc
      301 acgccaaaca tagacgatag aattgtttag cttttttgta ttttaacata aacagaaaga
      361 aaccaatatg gatttatatt tttgaaaaac gctggtcgga acggcgggat ttgaacccac
      421 gacccctatc cccccagaat agtgcgctac caggctgcgc tacgttccga cgatagagga
      481 aatgtaggct ttttcgtaaa agttttcaag gggaaagtca tcgtgtgtgc gccgttgatt
      541 ttttttgacg ttacaaatcc attaaataac tgtctagggt gaataaaagg tagcttgccc
      601 aaaaatgtgt tggtagccct ttataaagag attttaaggt gtgttttttt aaaatatttt
      661 tgagggaagt cgtttctttc tcgttataga aatgcgtcaa aagacttgta agggggtgtg
      721 ttaaaagcac atgctggcat gtcaaaaatg ttcctatgat gaaataatct tctgggcgga
      781 gagacccttt tcggtaatgc ttcatatttt tttgcagata gtcattggtg atgagaaaag
      841 aggttgatcc gataaacgat cgccaaaaaa taaggctgcc cccatcaaaa aaatttttga
      901 acttcaaagg aggaggggtc ttatcaagta gttttagcgt gcgtatgcgc tctaggcggg
      961 atgtgaggat ttcgcaaaaa ctaaacgttg ccccagacgt gagatgattg gttatgtttt
     1021 tataactttc ccactctttt gggtaataag ttgagagaat acccggaaga ttttgatagc
     1081 taatccactt atatgtgttt ttcaaaaggc tgatagaaat tgtttcctta agtatattca
     1141 gccctaaaac acctttgatg cctcttgtat tataaatatt tttgagtgtt ttaaggaaat
     1201 tattttgatg aatttgttgc gaggttgtaa tcgtttggct gaataatttg agcgtatttt
     1261 ctgcgacgac gcggtataca attgtttggg agagtggttt tttagaattt tcctccggat
     1321 tagacgcatg gcttaagtgg atgcaccaca gaaaaataaa taaatttttt atcattttcg
     1381 tcaaaacaca actccttacg ctggtatgaa ccagatcaag tgaataaagg ttgagcaata
     1441 agctctctca gctcccttga gtttataggc aaaagagcac cctcggcgat gacgccaatg
     1501 tacacgtgtg gataactctt gtcaaattta cccatgaatc ttaaccattt gttaagaatc
     1561 tctcttccac aataaaaccg tcaaccaatt ctggtttttt aaaagggagt ttttttatga
     1621 aaagaatagt atccacagcg cttatcctcg ctctttccac gggtttttct tattcttctg
     1681 agaataataa tgagaaaaaa aaggaagagt ttattgagat tgaagagaat agtaaatcta
     1741 aaaaaaagaa ttttaagata gaaaaaaata ttgatacagt agataaatgg aattttctta
     1801 agaataactc aacaaaaagt cttgaggatg taagtaataa gtacgatgaa ggtcaaaaag
     1861 aaattgacca aaagaaaaaa gaattaagtg aattgcaaat cacaaatgaa aatctt
//
LOCUS       DAIAHG010000178         1905 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_178_length_1905_cov_3.560722, whole genome shotgun sequence.
ACCESSION   DAIAHG010000178 DAIAHG010000000
VERSION     DAIAHG010000178.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178_length_1905_cov_3.560722"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          644 a          394 c          398 g          469 t
ORIGIN      
        1 aatattcata atgccgatga ttattttaaa atgatcaaaa aaattgccaa aaataatggt
       61 attctttttg agggagatat aaaagagtga aggctggtta tgttgccctc cactcctttg
      121 ttttaaataa gaggaagtag gaaacaagtg tgagtgtgaa cataaaaagc gcaagaatcc
      181 taaacgtacc tgcatagagg tagctcacac cggcaagacc cgcagctgag ccgatgagcc
      241 tgccaaaatt aataagagca gcggcccttg aggaggcatc gggaatacaa ctgagggcga
      301 ctggataaag aatatttatc gggaaaacaa aagggataac gtagaggctc acaatcaatg
      361 taattatcca cggtgtgtct ttgacgccaa gactgatgag tgcaatgaaa acgcccgcga
      421 gcatcataag gtgagaggaa agacgcagac atatctcacg gcgcatatgt ctaagaacaa
      481 cgggactgat caaactcgtc gcagaaaacc caacaagaat agcaccttga taaaaaccaa
      541 agtattgaag cggaacgccc atgctttcca tatagaggat ggggcccatc cccacaaaag
      601 tccagtggga tgtaatcagt aggcaaaaaa tataaaaata gcgcctgtat tccttggatt
      661 ttaaaagaga taaatagctt ttgagctcaa cttttacgga tggctcaggc ttgcctgttt
      721 ctgggataaa caaaagcgtc atgatgagcg aaatgatacc gagaatcaaa agaatcataa
      781 agttcgcgcg ccagccaaag tacatattaa tataacttcc taagatagga gcgcccgcca
      841 tgccaacagt ggtcatgcca tttaaaatgc ccatgcgccc tgcctgtttt tcaaggggcg
      901 ttacatctgc aatcacaaca aagccaagaa cagcaggccc tgccatgcca atgccttgga
      961 aaaagcgccc tatgacgagc catgaaaagg tcggcgccca cacgcacatc gcacttccga
     1021 tcagaaaaat cacaagcccg caaagaatca ggggtcgtct gccataacga tcaccgaggc
     1081 ttcccacata aagagagctg atgcaatagc tgataaaatt cacagaaagg gtcagctgta
     1141 cctcaaaagg ggtcagctta aagagacgaa taagctctgg aaaacttggg ataaaaatat
     1201 ccacttccat aaaggccaaa atgcacaaaa caaaaatcgt tataaaaagc ataaaaaccc
     1261 ttaggctgaa ttttttaaaa ccgcttccat tgttgcactt ttatggggcg ggacctatca
     1321 aataaaaaaa gaaacacccc acatcgtatc tggggtgatg cactttttac acatgaggtc
     1381 attcatcatg cggtttttct atgggctcag catgcagcac aagagagtca aatttttcgg
     1441 gaaaacgatg tacaccaaga atctcatact taaaaggggc atcgagagga gtgaactttt
     1501 tcatgcactc taaatcaaca tacccgtagg cacgtattgc gtaattatct tttttctcat
     1561 ctgtttttag gcgggcttgt aggtgtaaaa aacccgtttt tccttttgta aacgctggag
     1621 ccatacattt cttagagtat tttaaaaatt gtttgccaaa agttgcgttc ttttcaattt
     1681 ttttaaaaaa ctggcgttcg agacgttttt cttcgtcctg tgtatcgaat tctttaaatg
     1741 ctccttcagt cttttcatga atattttcgc ccattttcga cgaataatag atgttatagg
     1801 gtggatagaa aaaatccttt attgaatcaa aaattgatgc agatgcgtca acacttcccg
     1861 ctaaaaaagg gatgatgact aaaaaacgaa aacgcataaa aacct
//
LOCUS       DAIAHG010000179         1852 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_179_length_1852_cov_4.173521, whole genome shotgun sequence.
ACCESSION   DAIAHG010000179 DAIAHG010000000
VERSION     DAIAHG010000179.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179_length_1852_cov_4.173521"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          530 a          365 c          439 g          518 t
ORIGIN      
        1 cgtggtggcg gtgggtaggg aaacgccacg ggggatgacg tagggacccc gctagggggg
       61 agtgtgttcg ttgagggacc ccgctagggg ggagtgtgta ttttagtcgg gacgagattt
      121 aaaaacccca ggcatattta tttttctcac ttcacatatg tcaataatta taatacatac
      181 tgaattcgat aggatgcggc gcatgctcaa acgcatgaac atcgcgcatt ttaaggtcga
      241 tatacgcctc aatcaaatcg ggcgtaaaaa cattgccttg gcacaaaaag gcataatctt
      301 cttcaagaaa gtggagtgcc tcacgcagtg aagaggccac cgttggaaaa cgctttgcag
      361 agtgtgtatc ttcaaaaaga ttttgatccg ccgcagcacc agggtcaatt ttatttttga
      421 tcccatcaag tcctgctaaa agcattgcgg caaaagctaa ataaccacag gctgtaggat
      481 cgggaaaacg cacctcaacg cgtcgtcctt tgtcattaaa cgccataggg atacggcaag
      541 aagctgagcg attgcgcgaa gaataagcac aaatcacagg ggcctcaaat ccgggaataa
      601 ggcgtttata gctgttggtt gtggggtttg taaatgcatt gattgctttg gcgtgtttga
      661 gaatgccgcc aatatagtgc agtgctgttt gcgacagccc cgcataaccg tcgcctgcaa
      721 aaagaggctt tttatcttgc cagagtgatt gatgcacatg catacccgaa ccattatcgc
      781 ctgcaagggg tttgggcata aatgttgcag tacagccata cgtattggca acattacgca
      841 ccacatattt ataaatctgc actgcatctg cgacatccac aagtgttccg aatttaaagc
      901 caagctcttg ttgtgatgta gcaacctcat ggtgatgttt ttccatgaca agccccatgc
      961 cgctcatggc ttgaaccatg tcgctgcgca tatcctgaaa agtgtcaata ggtgcacaag
     1021 gcatgtagcc gcccttatgt ccagggcgat aaccactatt gggttctttg cctgaggcgt
     1081 aaggaagctc attggagtaa agatgataaa aactagactc tggctttgtt tgaaattgga
     1141 cttggtcaaa aataaaaaat tccgcttccg gtccaaaata agcagtatca gcaatgcctg
     1201 atgctttgag ataaacttct gcttttttag ccacagaacg ggggtctcgc tcataggcat
     1261 ggcctgcggg atcccaaaca tcgcatatga caatgcctgt attatgtgtt gtaaaagaat
     1321 ctgggatgac ttttgaggta tcaagcttta aaagcatgtc agactcgtgc acagccttcc
     1381 atcctggaat cgatgatcca tcgaaagcga caccatcgtg gatcattgcc tcgtccactt
     1441 gatgtgtggg aaatgtaatg tggtgccaag tacctaagac atctgtgaag cgaaaatcaa
     1501 taaactcaaa agactgaaga gattcaataa attgctttgt cataagatag cgctgataaa
     1561 aactattttc ttgattgtag acctacctag caaaagcgac aagggggtga aagtaaaaag
     1621 ttaaatgaaa tgattttcaa gggcgatttt ttcatgtaaa gggttttatg gcaaaattta
     1681 tggttttttg taatattttc tttgtttttg atgattctag tagcattttc catttcttgg
     1741 acgatatttt gtgggttttt catgtaaaaa atgggttttt gacccagtat tttgaaaatt
     1801 tcgataagat ccatcgtatt ttttgctaaa aaaagggggt tttcagtgaa aa
//
LOCUS       DAIAHG010000180         1841 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_180_length_1841_cov_3.014739, whole genome shotgun sequence.
ACCESSION   DAIAHG010000180 DAIAHG010000000
VERSION     DAIAHG010000180.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180_length_1841_cov_3.014739"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          632 a          308 c          463 g          438 t
ORIGIN      
        1 gcaaagacgt aataagaatg caaagtgcgg cggttgcttt tgtatcacca tgctccatca
       61 gggataaaag aacgacgccc agtgtttgtg tctctgacga ggggaggagg gaggatactg
      121 tgacttcgtg aagaatgagg ataaagacaa ggaaacttcc gttaaaaagg cttggagaga
      181 caagggggag aaaaatacgt cgtacaatgg aagaggtcgt agcgccacag cttttggcag
      241 attcaatgag tttatacgaa aatttatcac ttgccgcatg gacaatgtta aggtgaaagg
      301 caaaaaagcg gatcatatac gcgataaaaa taataaaaag cgtattataa atcatgttga
      361 gttgtaaaaa acgcatccag tcaaagggtt tgaggacaag taaaatcaga ctgatggcta
      421 aaatacttcc aggcataagg tagagggatt gaaaaaaaat acgcgccttc gcgaaaaagg
      481 ctcgctttgt gataagagca ccatattcga aaagcccact ttggaaaaaa agcacaaagg
      541 ctgtgccgag tgcgaggata aagctgttga aaaaggcatg gtggatattt ctattaccga
      601 taatgtagtg atagttttcc agtgaaaaat ggctcatcga aaagctcatc acgccttttg
      661 ttgtaaagga tgtgaggagg agtttgatca tcggcagaat aatgctgagg cagataaggc
      721 agagaaggcc atagataaaa acgcgggtgt gcttagagtc aaaggtattt tttgtgagct
      781 tttggcttgt tacacgccac tgccatgcgg ggcctgtgag tgttttgaga acataagttc
      841 cggccaagcc aatgactaaa agaatcatgc aaataagcac aatatcatcg attttattta
      901 cgccaaaccc tgtgagtttt gaataaataa gcgttgtgag tgtcgtaaac tgtccaggaa
      961 tcccaagcag cgcaaagaag gtaaagtgac cgagtgctga aaaccaaatc agcatggcag
     1021 atttcacgac ggcaccgcgc acaaggggga ggatgatttg tgttaaaaga actctgggcg
     1081 atgcaccaaa actttttcct gcctcgataa ggtgaaaggg aatgcgtttg agctgatcta
     1141 aaatcataaa aaagcaaata ggaaaaagat gaatcgataa aaggataatg ctggcggttt
     1201 gggtgtacat gggattggga atattcaacc aatgattgag atgaatgccg atatcaatcc
     1261 atgcaattgc taaaaaggtt ggcggcaaaa aaagaattag aaagatgata aacgaccaaa
     1321 tgtgaaggcg gtgtgtgtct gtgagcccga cgaaaagtgc aagaacaagg ccgacacttc
     1381 ccgcaatcaa tgtcgtaata gaggcgatac agatgctgtt aaaagctgcg cgcaaaaagc
     1441 gggggtcttt gaaaagatca ataagggtgt tccctgaaaa aatcagaatt tttgaaaaac
     1501 tcagaagctt tatcaatggc aatgcgcata caatgacgag taggcttgca aaaagaataa
     1561 acggattgat aaaaatgtat cgtttaatga atggctttag aaaactcaag gcgatcctgc
     1621 tctatgcgat ttaaaataat attagcatcc acgggaattt cctttggcat cccaagcttc
     1681 tcaaagcttt ttgggggcgc aacatccgtg cgaatggggc gataaccaag gttaacgagt
     1741 gtctgctgtc cttccttaga taagataaat tctataaaaa gcaatgcatt ttttttatga
     1801 gaagatgttt taagtaacgc aatgggcgct cgaatcaaag g
//
LOCUS       DAIAHG010000181         1708 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_181_length_1708_cov_3.174739, whole genome shotgun sequence.
ACCESSION   DAIAHG010000181 DAIAHG010000000
VERSION     DAIAHG010000181.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181_length_1708_cov_3.174739"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          565 a          344 c          323 g          476 t
ORIGIN      
        1 tgaaattgac cttaaaaagg cgagtaaaga gactatccaa gaaaaattaa aaaatcttga
       61 agcgattcag gaaaatgatc gcaacgccat caagaactat gaagaaaaaa taaaatacct
      121 caaggataaa attgataaaa cgcaaaaaga aatagaaatt gtcgagtacg agttgctgtc
      181 tcctcaggaa aaaatgtcta gcaatgcgtt tgattatgtg aatagtggca gaggagaatt
      241 cttttctcaa actacagagg attttaatca gggattactc tcaaaaaaag attttgatga
      301 tgttgcacac aaggcaatga tgaatgccat agctgaaaat ggtgatgatc gaatcactcg
      361 cgcaaagaca ataaagaatt ttttgaatga cttattaaaa gaaaaaacaa aagaataaaa
      421 agatgaaatg atggggggtg cttacgtttt aaattcctct aaagcgaaca cttcccctca
      481 caaaacaaga gccattaggc tttagaaaat tttaaaaaaa acttataaaa aaaagtggat
      541 cattccgtcc tcctcctttg agttgttttg tagtagagtg gcgaataaaa atgcattttt
      601 taccgtgccc atgcctttgc ctaaaattgt tattgccacc cataaccctc ataaacgcga
      661 tgatttaatt cactatgtac accacacttt tgagtgtgtc ccaattgctg attttacgtt
      721 gcacgaaccc ttggaaacgg gagagacctt cgaagcaaat gctctgttaa aggcacgctt
      781 tagctatgat gtaacggggc tgccttctct tgcagatgat tcagggtttt gtatgaaaaa
      841 tcttaacggt atgccaggca tttacgcagc gcgctttgca atcaataaca aaggtgaaac
      901 tgattatctc catgcgttta atacactgga gcaacaactg ggcgatcaag atcctgcaac
      961 ggattttgta tgcacgctcg tttataagga cgcgcatggt gagaaagttt ttcaaggcac
     1021 tgtgccgggg ttttttgatt tttcgaaaaa gcatacgcct ggatttggct atacacctct
     1081 ttttgttcct cttgagaata atcctcaaaa acttagcctc gccgatatgg gcgatcccca
     1141 gcgtcgcacg ttttcagccc gcaaaaaagc agcgtttgac tttctccaat gggttacaca
     1201 gtccaaaaaa taatgtttca ctttcttaaa aaaacaacag tcgcaataca tcaccagcat
     1261 tccttagaaa attttgaggg aaagccatcg cttaaaatta atccgcgctc aaagcgcatt
     1321 gtttttcgtt gtgatcaaaa aacgggtaaa cctgttattg tccttccctc ctctttttat
     1381 ctgaaaaaaa tcaataccta cgaagcatcc tatcatcagt ggtatgcgaa ccttaaaatt
     1441 aatccgccgc gaacactttc aacgggaagc tcttttccct acaaaggaac acctcatctt
     1501 ttgaatttta cccaagaaaa actttactcg acgcctcaaa tcatccacga cgatggtatt
     1561 ttcattatta aagggcgcgc tgaaaaagat cttggtcctg cgcttaaaaa ctatttagtg
     1621 gcagcagccc ttgattatgc aatggaaaaa agtcatctct atgcccataa gcttggtgtt
     1681 gagctcacgc atattgctgt taaagata
//
LOCUS       DAIAHG010000182         1645 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_182_length_1645_cov_3.308036, whole genome shotgun sequence.
ACCESSION   DAIAHG010000182 DAIAHG010000000
VERSION     DAIAHG010000182.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182_length_1645_cov_3.308036"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          508 a          349 c          334 g          454 t
ORIGIN      
        1 tataaaattt tagtgaaaaa tatgtttaaa atccttcgtc tctcgctcat gacccttttt
       61 tttctgggca ttgcaggtgt tatcgtttat taccaaatcg aaaaacttcc tgtcgttcac
      121 aatgcagtgg atgaaatgga tgtggtcggt gggccttttg atcttatcga tcaagatgga
      181 aaaccctgca aaagtggtga ttttaaaggt aaatatctga ttgtttattt tggctatagc
      241 cattgcccgg atgcctgtcc gcgggcgctt cgcaccatta cagaagcact tacatccctt
      301 aagcgggacc gtgatcaagt cgcagccgtt tttattacca tggatccaag tcgcgatacg
      361 gttcaagaac tcaagacata tagcgaaaat tataatgaaa atatgttttt cctcacaggt
      421 gataaaaaag ccattgatgc ggtggcgaag gcctataaaa cttatgcgaa aaaagttaat
      481 agcggagaga caacagagta tctgattgat catagtacat tgatttatct tcttgaccgt
      541 gagggcaagc agattgctta ttttatgcac acagttgaac ctgagaaact ctcaaatgcg
      601 ctattaactg cactcaacca agagcataaa cacaatcgca cagcctagac tataacagaa
      661 ctaggatagg atttgaccct caacttccga gactttgtta aggcgttttt ggagttcaag
      721 gcgcttgtct tcaagtgtct cagcatcatc gtgaggggta atcgtaatgg cctctgctgc
      781 atggaaaaat ccctttgaaa agggaagagg aaagagaaat ttatcccaag accgaaggcg
      841 tttatgtctt tgaataaaat acccgacggg cacgatatga ataggttttt cctctgttgc
      901 agcaagtttc gctaaggtta aaacgcccat agaacagatt tgatgggggc cacgcggtcc
      961 atcgggcgtc acaccaatca cagcccccgt tttaatcaga tcaagaattt gcaagcctgc
     1021 aaccatcccc ccgcgtgttg tcgatccaaa aacactttga atattgaaat gctcaagaac
     1081 acggctaatc agttgaccat cacggtgctg tgacagcaac atataaaaag gtttttccca
     1141 ccaccatgca caaggcaaca tggccaaatg tccgtgccaa aaagtaataa aaaaaagact
     1201 tttctctgac acaagtgaat caatcacacg atgcccctta aactcaaagg agcttgtacg
     1261 ataaacactt ttgatataca gcgcgataag aaaggcgagt gttttttgca cactgttcaa
     1321 ggccataatt tttttgatat atttcattag ttctcattat ccttcaaaga atatgtatgc
     1381 cagaaaaccc ccaagaagtg tacgatgaca cgaatgtttt tgccaagatt ttacgtcagg
     1441 aaatccccgc aaagattatt tatgaaagta ctcatgcgtt ggcttttttc gatgcttttc
     1501 caaaagcacc tcttcacgca cttgtgattc ctaagggaac atatcgcgat ctcaaagact
     1561 ttttagccca tgccacaaca gaggaaattc tggatttttg gaaaactgtg caagcaacgc
     1621 ttgataaact tgatctcaaa gaagg
//
LOCUS       DAIAHG010000183         1581 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_183_length_1581_cov_2.968750, whole genome shotgun sequence.
ACCESSION   DAIAHG010000183 DAIAHG010000000
VERSION     DAIAHG010000183.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183_length_1581_cov_2.968750"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          475 a          299 c          308 g          499 t
ORIGIN      
        1 aaaaaaagtg gtaaaaatcg cttttttcat aggattcagc gtgtttttcc taacaattat
       61 agcttcaaag tggaaaatac cacgaaaaaa tgggcgaaaa atcgcttttt tcataggaat
      121 gagcacaata tccatgaaaa atacagaaaa agagggattt tttaatattt ttttacagaa
      181 aaatctgatt ttatctgtct aaggccgtag cgggggattt ccattgaaaa aggagacttc
      241 ccgccgcttg tttccactaa atgatgtgct tttttccaaa attttcgatg caaaacatct
      301 aaaatattca tcaaaatcaa cgcaaaacgg gtttgccttt ttgtgaaata aaatctttta
      361 aatcacgtgg aaaaaaatca ctttttacac agcacagtga taagagcctt gaccccttgt
      421 gaggattcat tttaactaaa atagtaatta actaagacgt ttttggatat cccctttgac
      481 agaaatctca tccccctctg accgctcggt cctgttgtgg tcaccctctt ctgcttatat
      541 tgagcagaca cttttgagca attttatgaa aagtgttgga aaatctgact acaaagaact
      601 ctacgcgtgg tcgattgatg atatcgaagc gttttggcaa tctgtttgga atttttgcag
      661 aatcataggc gatgcccctg aaacctttac aattgaacat aaagaagatg ttgagaaggc
      721 tgtgtttttc aaggacgcga agcttaattt tgcggaaaac cttttaaggc gacgggatga
      781 tgcgacagca attacttttt actgtgaaga tcacatgcac cgcacactct cttaccgtac
      841 actttataat gaggtgagtg ctctagagca atttttaaaa tccttgggta tcgaagaaaa
      901 tgagcctgtg ggatgctatc tgcccaatat ccccgaaacg attataggaa tgcttgcagc
      961 caccacacac ggggctattt ggtcaacctg ttcacccgat tttggcgcct cagcgcttat
     1021 tgatcggttt ggacaaatta gtccgcggat tctttttatt acggatgggt actattataa
     1081 tggaaagatt tttgatgttt tgtcaaaaat tcctgaaata cttgaggcaa ttccaagcat
     1141 tgagcatatt attcttatcc cttatgtcaa aaataatgac atagatttta aaaatacgaa
     1201 ggataaagtt cacattcacc tgtggcagga tattatcaaa aactttccag ccaaagagat
     1261 tacctttaaa cgttatccct ttaatcaccc tctttatatt ttgtattctt ctgggacaac
     1321 gggtgtacca aagtgcattg tgcatagtgc ggggggcacg cttctccagc acatgaaaga
     1381 acatcagctt cactgtgata tcaagccgga tgatgttatt ttttatttta caacctgtag
     1441 ttggatgatg tggcattggc ttgtctcgtg ccttgcaagt gaagcatctc ttttgctgta
     1501 tgatggcgct ccaacatatc cgcatatgga ttatcttttt gagcttgcag agaaaaataa
     1561 tgttaaattg tttggcacct c
//
LOCUS       DAIAHG010000184         1558 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_184_length_1558_cov_3.248481, whole genome shotgun sequence.
ACCESSION   DAIAHG010000184 DAIAHG010000000
VERSION     DAIAHG010000184.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184_length_1558_cov_3.248481"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          413 a          294 c          312 g          539 t
ORIGIN      
        1 tcaatatttt tttaatgatt tcctgagcac cttcatcgtc aatcgtttga attttctttt
       61 gggctctctt tattacctgt tgcctaacaa gataattaaa ggtgttctca agaaattttt
      121 cacccccttg aaatattttt tcttctttcc gaaggatact ctcgtgttct tttatctgag
      181 tttctaaatc gtttttaatt tgttgtgtgg tgataaagtc aaaatagtta tcccgtgctt
      241 tgtcaagtcg gtttcgttcc tcttgagttt caggttcttt ttgactagct tcaccaagtt
      301 ctttttcgag agcctgaaaa tcagttgatt ctttttctat atcgttaatt ctttttttta
      361 tatttattaa tttgattgac agtttcaaaa gtattttcat tgtatttttc tttaattttc
      421 tccatgaatt tattttttgt ggattcttta atataattct tcccatcaac aaattcaccg
      481 agggtcattt tatcccttcc caaaagattt tgaggaaaaa aagcgcttac aaaaataagg
      541 cttatcgtga atgattctaa tttatttttc atttgttatc tcttcatact tttattaatg
      601 ggtttttctg tagaagaaag ttttcaatat gtctacatat atttaactcg atgcagcttg
      661 caaaaaaatg aatagacgct gctattgaaa aaacattaga atccatccta aaaatggtaa
      721 aaaaacaatg acgcattctt tttggattat cctcgacaaa ccctatggga tgacatctaa
      781 tcgggccctt tcaaaggtta aaagactttt tgggttaaga aaagcggggt tttcaggcac
      841 ccttgaccct cttgcgacgg gcgtgctgcc tattgctttt ggtgaggctc ttaagacgat
      901 gcattatatg actttttcta aaaaggcgta tacatttcag gtgcggtggg ggcaggaaac
      961 cacaacagct gatagagaag gggaggtgat acaaacttcc tccatacgcc ccacttttga
     1021 gcaaattcaa cacatattgc cgcaatttac cgggcaaatc acccaaacgc ctcccattta
     1081 ttcagcgata aaagtcgatg gcaagcgtgc ctatgatctt gcccgtcaaa atgttgaggt
     1141 aaaacttaaa gaacgaatgg tgacgattta tgatctgcag ttgataagta ttgatgatga
     1201 agatcatgca gcttttgccg tagagtgcag tgctggaaca tacattcgaa gtcttggcgt
     1261 agatcttgca aaggcgcttg ggacggtggc gcatctttca cagttacggc gcacctctgt
     1321 tggctgtttt tcacttgaaa cgtcgatttc actggaatat ctcggtgaaa ttgtgcatac
     1381 tgacgccatt cttgcgtata agcattccct tggaatcggg ctggacgaca tcctggcggt
     1441 ttcaatcaca gaggatgact atacaaacgc caagcttggc aggcgaatac cttgtgcgtt
     1501 gcatcttgag gaggaagctc ttgtagggat gtgggtcgag ggtgactttg ctgggttt
//
LOCUS       DAIAHG010000185         1535 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_185_length_1535_cov_2.195473, whole genome shotgun sequence.
ACCESSION   DAIAHG010000185 DAIAHG010000000
VERSION     DAIAHG010000185.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185_length_1535_cov_2.195473"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          418 a          295 c          323 g          499 t
ORIGIN      
        1 cgtgatgttc cagattatct tgaggttgat cataaagcaa tgaagggaac ttacgtacgt
       61 acaccaactc ttttagaagt tccctaccct gttcaaatgg aacccaatct tgttgttgag
      121 ttttactctc gataattctt tgaaagggtc cttatttctt tttcccttct agggcgctta
      181 gctcagttgg tagagcagct gactcttaat cagcgggtcg aaggttcgaa tccttcatcg
      241 cccaccattc ttcctataag ctatttgttt tttgattatt gcagcatttt tgacacagat
      301 catttctctg tgaatcttaa attaaatttt atattctaaa agattagaga aatttgttat
      361 taaaagaatg actttaatct ctgctaaaaa tgtaaaattg atgaaaagaa tagtattcct
      421 taaggtttgt tgcctcctag tggtaccagt catgataagt gcgatggaag ataattatga
      481 taaaatcttt gatcaagtaa aaaagcaagt ccctgtgata gagggggtag agctccccac
      541 tgcaaaagaa attcatgcgg ttgagcccct ccatccttct cataggagat ttttagaaac
      601 ccttggcaat agagattttg aagggtatga gcccttgcgt gcccaaggtg gagaaaattc
      661 cctgctggcc cagaaaaaag aggacgctca ccgaagtggt gttggaggag agtgggttcc
      721 cttttgtgtt caagaaggtg acctttggtt tatccgcgtg gacacaggag aagcgaggat
      781 ttttatacga tccgtggaaa aagaggaata tatttatacc cttgatgaag gagaagacgc
      841 cttgccctcc ctccttcacc taatgcaaac tctttggtcg gatcttaacc ttgagattgg
      901 ttgagtgttt tggataattt gaggcacatg gtttgccgtg cttttcccca caaattccgg
      961 tccacacgtt tccttgggac gctgctataa tatgtatctg ttaaatgaag caacccactg
     1021 tattttttat gaaatttgtc cgataaaagg acaatatacg tcgtgccata gcttgtgcca
     1081 tgggttttcg cgtcatggag ggtaaagtga tgaaaattca tctctttatt atcataacca
     1141 atcggacaca gctttctttg aaggcgttgt agatttgttc gcttgcccgt gtcatccacg
     1201 cgcccaagat caataaattc ctttgcataa aaaagacaat agcgtccaaa gcgtaaggtt
     1261 ttataatggg gttcaaggtg aatatcaaaa aaggattcaa gctcaccttt cgtgagtggg
     1321 gcatcctttt ttgtatcaat agattctttg cgttctaaga ggacttcaaa ataactttcc
     1381 caataacttt cctctaattc aaaaatgcta ttgaggtttt tttttgtaag gccacgctct
     1441 ttttcccggg cttcaaaagc taagcgaatt ttttcttttt ttgccgcatg ttcttcggct
     1501 gtgtctggtt tataaggttc ttcataatca agggg
//
LOCUS       DAIAHG010000186         1521 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_186_length_1521_cov_2.730609, whole genome shotgun sequence.
ACCESSION   DAIAHG010000186 DAIAHG010000000
VERSION     DAIAHG010000186.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186_length_1521_cov_2.730609"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          519 a          336 c          301 g          365 t
ORIGIN      
        1 aaactcttgc ggaaaaatac tttggcccca ttccttcaaa ggatattcca aaacgcgcac
       61 gcaccgagga atctgatcac aaaaacacaa cgattacaat tgaccatgca tcgccccgca
      121 ttaaactcac aggaatcacc ctcagctacc tagcacccaa ttttaaaaag gataaacaga
      181 tggccctcgc gctcaccgtc cttgctcaaa ttatgggcgg tagtgcggtg tcgcgtttga
      241 caaagcaatt cgttgaagat gagcaaattg cttcagatct cagtattgac tatacctatc
      301 aaagcattga tcctcgggat ttttctgtga gtgtcacctt acttcccaaa attactgtgg
      361 aagatatgaa aaaaaaggtt tttgcggcac ttacgctcct tgctgaaaag ggtgtcacgg
      421 aaaaagaatt agtacgtgca aagcgagatt tatgtgcgcc ctttgctttt gcaaaagatg
      481 gcttggatgc agcatccgat gcgtttaccg ctcttgcgca tgatattccc gttgaggata
      541 tcgaaaatca agaggctctg attcaaagtg tgaccctaga acaagtgaat caggctgcga
      601 agttcctctt ttccaagcaa cctgctgtga tcttagtgtt aaagcctgag ggtaaaaatt
      661 ctgaaaagcc taaaaaagac taagctttct tggatctata aaaaagctga agagcgaggg
      721 agagaagtaa aacggctccc aattgatgca tgagtgcaag tttaaggggc acaatgaaaa
      781 tcaatgttgc aatgccaaga gcgacttgta aactcaccgc aaagaaaagt aaaaatgccg
      841 attgtttatc ccgaagtttc catactttta aagacatcca tacagcgtaa aagaatgtaa
      901 aaatacccaa tatacgatgc ataaattgaa cggtcacagg gttttcaaaa aagtttttat
      961 acaaagggga aagatgattc cattcccagg gtagccactc atctcccatc atgggaaatg
     1021 tgttataaag ctgtccggcg cgcagccctg ccacaaaagc gccataaatt atggtaagaa
     1081 aaatcaacat caagagcccg tggagcggcc cccttaagga tgaaagatta tagcgatcat
     1141 tctctttaac aagatcaaaa attgtccaaa gaaaaaaaca caataagaga agggcaagag
     1201 acaaatgggc cgcaagacgg taatgactca cataagggtt attgacaagg ccacttttca
     1261 ccatatacca ccccataaaa ccttggcctg ctagcaaaaa ggtcataaaa agaacgcgcg
     1321 ttttatgtgc actattcaga ttcttccaaa aaataaaaat tggcactaca aaaacaagac
     1381 caaggatcct cccaagcaaa cggtggctat actccatcca gaaaataaat ttaaatcctg
     1441 aaagatccat ctccatatgc tttagagcat actctggaaa agctttgtaa tgatcaaatt
     1501 cactcagcca tccttcctcc g
//
LOCUS       DAIAHG010000187         1503 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_187_length_1503_cov_3.008415, whole genome shotgun sequence.
ACCESSION   DAIAHG010000187 DAIAHG010000000
VERSION     DAIAHG010000187.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187_length_1503_cov_3.008415"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          378 a          293 c          348 g          484 t
ORIGIN      
        1 tttggaagag aggcgtgtgc gaggttcatt tttcaaggga tccaaaggat gaatgttctt
       61 ttttattaaa cttttttgcc ttgttggaca ataatttcta tttttttact tttttgtcta
      121 agcggttctc ccgggggtgt ttgagagcta ttgacctcat actggcctgt tttttgattc
      181 atcgacgcat acgcactttt aataaaagtt ttgccatcat aaatgcgcac atcatcaaaa
      241 aactcaatga tctctgtctt cgtatcataa acggctgtgt ctgaatgaat cgtgacgcca
      301 tcattgacaa ccataacatg gcccttggcg tgaatagcgt gaatatctgt ggctccaaaa
      361 ggtgctgcgt ctccctcttg tggcgctttt tcagggactt gatctttggg tgaaaaaaaa
      421 atcgtgagcg tgtccgatcg taagctttgt tctttgggag ggggtgtttg ctgaggttca
      481 ttttctggtg ctttgcggat gacatgcaca tctgtttcag caatgcatgt attatctttt
      541 ttttcgcatg ttactttttc cgccgtaatg accatctgag acgcatgcgc aaggggtgtg
      601 aaaatgaaaa aaagaatcca aaaacgcatc agtctctctt tatatcaaag gttagcacag
      661 gacgatctgt gagaacaatt ttgtctcctt tatgatagat ttcaaaggcg ccggcatcaa
      721 tctgtatttt atcattcgtc atatgaatgg attgatggcc aaaggcatcg cccttctgca
      781 gatcaatacg cgtgtggggc attttaaacg ctgtgtcttt gccataggtc acaacgacat
      841 ccccttcgag ggttaattcc tttgtatttt gatcaagttc gcccttaagc gctgtgactg
      901 tgacctcaag gttgtttgtg agttttatcg cataggaggg aagggtgaga aggatatgcc
      961 catcgctttc ccgtgttttc gcggactcgg cctgaatttt atacgcttgc cctttggcat
     1021 ctgtagcgtg gaagagtgct ttttttgcag ttgtttgtgt ttttgctgtg ggaggggcat
     1081 tgtgcaaagg gtcaagggcg tcattatctt tttccgattc cataagatca ttcgtgtgca
     1141 caagataaaa aataaaaaaa gtcgtgagga ctgaaatgat aataagaccg tatttgagtt
     1201 ggcgataaag acgaaacatg ctgtttataa aacaccgctc ttaaggaaat cgtggatatg
     1261 aataaggcca atcggttttt tttcctcatt aataataaaa agggaggtaa tcgatttttc
     1321 ctgcagcaac ctcagggcat cggccataag aatgtctttt gtgatggtaa tcgggtcact
     1381 tttcataata tctctggcgc aaaggctcat gagatctttt ccttgggtga gagagcgccg
     1441 aaggtcacca tccgtaatga ttcctaaaag aacgttcgcc tcgtccacaa tgccaataca
     1501 ccc
//
LOCUS       DAIAHG010000188          967 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_188_length_967_cov_41.638202, whole genome shotgun sequence.
ACCESSION   DAIAHG010000188 DAIAHG010000000
VERSION     DAIAHG010000188.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188_length_967_cov_41.638202"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          252 a          216 c          253 g          246 t
ORIGIN      
        1 ttcttttgga aaagatacta aaggtaagca acgcctggtg attaccgata ccgatggcat
       61 tgcacatgac tatctgattc ctaaggataa acacgttact gttcatgatg gtcaagtggt
      121 aaacaaggga gagacgattg tagatggtcc tgctgatcct cacgatattc tgcgcctgct
      181 tggtgtggaa gcattggctc gttatattac cgatgaagtt caggatgttt atcgtctgca
      241 gggggtaaag atcaacgaca agcacattga ggtgattgtt cggcagatgt tgcgccgtgt
      301 tcaggttctt gacgccgggg aaacacgctt tattcctggg gaacaagttg agcgcgccga
      361 gcttctcgct gaaaacgaaa gtgtcgtttc tgagaataag cggcctgcca cctatgaaaa
      421 cgtattgctg ggaattacca aagcttcctt gtcgacagat tcctttattt ctgcagcatc
      481 tttccaggaa acaacgcgcg ttctgaccga agcagccatc atgggcaagc gcgacgatct
      541 gcgtggctta aaagaaaatg tcatcgttgg acgcctgatc ccggccggga caggtttggc
      601 gtatcacacg gcacggcatc gccgtcctag tccggctgcg caattgggtg ttcctgaaga
      661 gtttctgatg gtggagcctt tgtcgccagt ggaaagcggt gaatcaagta ttgcttgaca
      721 aggttgaatg aataaattaa aatcgacagt ctttttacgg gctacattaa ttggcccgca
      781 gtccggctgt catccgagaa cgataaggtt tagaaaatgc caacaatcaa tcagttaatt
      841 cgtaagccga gagtggctgt caaagccaag agtaaagtcc cggctttggc caattgcccg
      901 cagaaacgtg gtgtttgtac ccgcgtctat accacgaccc caaaaaaacc aaattcggct
      961 ttgcgta
//
LOCUS       DAIAHG010000189          823 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_189_length_823_cov_7.912869, whole genome shotgun sequence.
ACCESSION   DAIAHG010000189 DAIAHG010000000
VERSION     DAIAHG010000189.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189_length_823_cov_7.912869"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          228 a          112 c          158 g          325 t
ORIGIN      
        1 attattaata ccggcgcgaa tttcgcaata cgtgcacttt atcataatgt tataggtggg
       61 tttgaaaaag gacttatcga gtattttgga cctcttgggt tttataggat atttcgaagt
      121 atgacaacta acgtgcagtg ggtacatcaa gggaattata gttattattt attaattata
      181 gaattccttg cattttggtt attacttttt atgagttttt tctttttact agggtaatca
      241 agtttttgtg cacagaattt ttgcgttgtc ccgcgaacgg tatatcgctt cttttataat
      301 taacaggtcg tcttgtatta agttcgggtt tttataaaag gagttctggc tttattttgg
      361 agtaaaaggg atcgaacctt tgtatgtcgg tatcaaaaac cgatgcctta ccacttggct
      421 atactccatt tttaggaaac taaaaatgtg atttaaattg gatgtttatt gttataaaaa
      481 ttttgggatt taattccata tatcgttgca tcaatgacac aattaatgca cggtcagcca
      541 ttgtgccatt aatatggaac gctgcagtgt atagagtttt tttaaactgc tcttgtgagt
      601 ttttattagc gtgtggtgag cgtagtaaac tccaatgtgt agtactgcgt ttgctcatgg
      661 taatattttt gcaagcaccg aatgtataac gtagatcgac gatgcaaaca ggaattagtc
      721 gcttttcaaa cgttttaatt tttatttggt atatcatttt ttgtactaat aatacgttat
      781 atattaactt tttggaatta ctacttatct tgttatttag att
//
LOCUS       DAIAHG010000190          817 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_190_length_817_cov_25.214865, whole genome shotgun sequence.
ACCESSION   DAIAHG010000190 DAIAHG010000000
VERSION     DAIAHG010000190.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190_length_817_cov_25.214865"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          137 a          272 c          254 g          154 t
ORIGIN      
        1 gtggagcagg tgccgcaata ccacatcgag ttccagctgc cggcgacgcc cggtttcgcg
       61 tggcaggcgc tggtggcgca tcccgacatc ctcgacgcgc gcccgcgcgc ctgcgactga
      121 tcgcggcggc cgcgccgggg cgcccggcta caatgcgcgc acgccggcat cgcccggtcg
      181 gtagcgccgc cgcccgtccc gtacgcgatt gcgggtggac ggccgcgggg ccggtgcggc
      241 aaagtcttcg ggctgccagc gggtagactg cgggtcgggc cgagcatggt tcgagcaaga
      301 aaatacgccg tgccgcttta gctcagtcgg tagagcaact gatttgtaat cagtaggtcg
      361 tccgttcgaa tcggacaagc ggcaccattt ccccggcgtc agtgctcgcg actcccatca
      421 acccggagat agctgcttgc gtttggcgag gtagtggcgg cttttttagg atgtcgcgct
      481 cctcggtgac ccgccgaagc tccaccttca gacgccgaac ttcggcactc tgatcgagtt
      541 ccgacgctgc acgacgcccg gtttgccgaa cttgcgcagc cacgcgtaca agctgtgggt
      601 ggtgaccccc agccgttcgg ccacgtcggc caccttcaac tcatccgtat accacttgct
      661 gctcatggac acctccgcag tagccatttt ccatggcccc gagatgtcta ggaaaccctg
      721 ggcgattcag atcacccgcg cagaggcgcc gcgctgagga ctgtgttgac cattctcgtg
      781 gtgaatgact atcatccacg gcatgaaaac ccttccg
//
LOCUS       DAIAHG010000191          791 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_191_length_791_cov_5.442577, whole genome shotgun sequence.
ACCESSION   DAIAHG010000191 DAIAHG010000000
VERSION     DAIAHG010000191.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191_length_791_cov_5.442577"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          165 a          264 c          227 g          135 t
ORIGIN      
        1 agcagactgc ccagcccacc gcgaacagca gcaccggtcc ccgggacgcc cccccggact
       61 agtcaccacc atagccaacg gagtgcgtcg gagctagaga attggttggg cgagatccgg
      121 cctgccatca agtcccgcgc aggagggccg atgcggcgct ggccgatcca tgatcaccgt
      181 gccgccgcat gcgccgctcc gactgaaccc taatttttcc gtgtccaaaa tagagttcac
      241 tctaatctac acgcgggcgc tcggctgatg ccctgtgcga gtggacctgc gctggagcga
      301 tccaacttca ccaccaaggg tccgtcaacg ccaacacctg cgcgagacaa cgcgacgccg
      361 agcgagccgg cccgagaacc catgagaaat gacgcgacct cagaatgccg cgaatcgcag
      421 cacgaaacgt agtaacgctt tgatttataa cgatatttct ggcggagaga gagggattcg
      481 aaccctcgaa gggcttttga cccttacacc cttagcaggg gtgcgccttc gaccactcgg
      541 ccatctctcc ggcttcttga ggcgggatcc ccggatgagc acgtacgctg acgcaagcgc
      601 cgccgcgtgc gcggccagga ggggcgcatg atactggaaa cgcgcaagga atgggacgcc
      661 ccggcgagcc agccgactca gatcacggcg tcctgcactt ctcgcttgac agcctcctca
      721 cccattgact cggaggtctc gacgcccgag cccgtgtgct gctctttttc ccgctggata
      781 cgcttgtaaa t
//
LOCUS       DAIAHG010000192          753 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_192_length_753_cov_13.942308, whole genome shotgun sequence.
ACCESSION   DAIAHG010000192 DAIAHG010000000
VERSION     DAIAHG010000192.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192_length_753_cov_13.942308"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          122 a          233 c          258 g          140 t
ORIGIN      
        1 atccgcgagg gcggccgcac cgtcggcgcc ggcgtcgtgg cgaagatcat cgagtaagtt
       61 tcagcgaccg cgggccgcgc ggatctccgc ccggcccgcg ccgcatgccg gcggggtgct
      121 gcaagcaccg gcgggccgcg gcgcaatggc cgatccgtcg ggtttcacag gggcatagct
      181 caattggcag agcgtcggtc tccaaaaccg aaggttgggg gttcgagacc ctctgcccct
      241 gccaccaccg gatgcagcac cagcagcagc acaacatggc caatccgaat ctagaaccac
      301 ccgtctccac cgcggccgac aaggccaagc tgggggtggc cgctgtggtt ttcctcggcg
      361 ccttcggcgc ctactacgcg ctgcacggcc agcagacctg gctgcgcgtg gtgaccctgc
      421 tcgtgctgct cgtggtcgcc ggcgggctgt ttttgctggc cggccccggg aaagccctga
      481 ttgtttatac tcaggagtct gtgcgcgagg ttcgcaaggt ggtctggccc gagcgcaagg
      541 aagccatcca gatgacggcg atcgtgttcg gtttcgtcgt cctgatggcg ttgttcctgt
      601 ggattgtcga caagatcctc gaatggggcc tgtacgacgt gatcctgggt tggaagcatt
      661 gatcggagcc gaccgcaatg gttgataccg tgcctgccgg ccagaagcag tggtacgtgg
      721 tgcatgccta ctcgggcatg gagaaggccg tcg
//
LOCUS       DAIAHG010000193          633 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_193_length_633_cov_2.598921, whole genome shotgun sequence.
ACCESSION   DAIAHG010000193 DAIAHG010000000
VERSION     DAIAHG010000193.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193_length_633_cov_2.598921"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          183 a          129 c          158 g          163 t
ORIGIN      
        1 aagtcaggtg cgtgaaatca cggatttgac gcaaggcggt atggcagcga gcgaagaaca
       61 agctgcgcaa ctccaagaat tatccgcttc gatcaatacg gtttatgaaa ttctaaagaa
      121 tttaaaggat ctcgcgaagt gaactcgtac agcgcaataa aaaagaattg gctaccaacg
      181 actttatgac ttgaaatacg acggttcgat cgctataata acgaagtgtt gtgtttcaag
      241 tgcttggagc tgtggtgtag tggcctaaca tgcctgcctg tcacgcagga gaccgcgggt
      301 tcgaatcccg tcagctccgc cagaatgcgg tggttgcgtc tcaggacgta attgccgcat
      361 tgtttttatt cctagactaa aggagtttgt ttgccttgca acacatactc gtagtggatg
      421 atgaagaacc gatcgcagag attttacgtt atcaattaga aaagtcgggt tatcaagtga
      481 gtgtcgccga agacggcgat aaagcgattc gcttcgctga ggaacacacg attgatctaa
      541 tattgctcga cctgatgtta ccgaaaaaag atggctttga agtttgcaaa gaaatcagac
      601 aattttccac tgtcccgatc attatgctga cag
//
LOCUS       DAIAHG010000194          627 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_194_length_627_cov_28.772727, whole genome shotgun sequence.
ACCESSION   DAIAHG010000194 DAIAHG010000000
VERSION     DAIAHG010000194.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194_length_627_cov_28.772727"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          127 a          176 c          183 g          141 t
ORIGIN      
        1 atccgcgagg gcggccgtac cgtcggcgcc ggcgtcgtgg caaaaattat cgagtgagac
       61 aaacgctctg gcttcgagct tgcatcgaag ccggttatgg gatgagcagt accgcggcca
      121 aacaaaaggt ttccacaggg gcatagctca attggcagag cgtcggtctc caaaaccgaa
      181 ggttgggggt tcgattccct ctgcccctgc cacaaccggg ttcagcacaa gcagtcaaca
      241 atggccaata caaccgtcga aaccgtctcc accaccgccg acaaagccaa gctgggggcg
      301 gcgggtgccg ttttcctggc cgcttttgcg gtctattacc ttttgcacag ccaggagttg
      361 tggctgcgcg tgctggcact ggtggcttta ctggcattgg ccgccgggtt atttctgctt
      421 tccgaacccg ggaaagccct gattgcttat actcgcgagt cggtccgtga agtgcgcaag
      481 gttgtctggc cggaacgcaa ggaagccatt cagatgacgg caatcgtctt cggcttcgtc
      541 atcctgatgg cccttttcct ctggatcacc gacaagtcgc tggagtggat tttctacgac
      601 ctgatcctgg gttggaaaca ttaatcg
//
LOCUS       DAIAHG010000195          610 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_195_length_610_cov_2.103189, whole genome shotgun sequence.
ACCESSION   DAIAHG010000195 DAIAHG010000000
VERSION     DAIAHG010000195.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195_length_610_cov_2.103189"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          120 a          202 c          175 g          113 t
ORIGIN      
        1 cccctcatcc tcgcgggtgc cggagttggc cttctacctc ggcctctcgc gtcgtacgcc
       61 gcggctctcg ggtgcagtgt tgcagaactg accccgcgcg tccagcgctc tatcgttgta
      121 gttcaccgca agggtcctct gacccccggc gcgcgcgctt tccttgacct gatcgacgag
      181 accctcttca acgtcaccac ctagtgttcc gtcagtcaaa acgtagaagt ggttagaaca
      241 tcgggacggc atcgaaactc gcggcgccat ccttgcacgt gtccaacgag caacgagcaa
      301 cgagcaacga gcagtggtga cgcgaccgtc gcgatcacgc tcactggcgc atcgaacagt
      361 gccgcaagcg aaggctcctg atgtcagccg atggcgaagc cgtctacgaa gtcgctcgtc
      421 gcattgaggt gggctccaac ccggtgcgac agtggcgact ggtcacgtgc tctatgacgc
      481 cagggaccga cactcgtcca aagaggtcct cgccttcgtc aagttcattg acctgcacgt
      541 ggcaaaggac ctggagatcc acgtcgtgcc gaaaaacctc tcggcgacaa ggccgatccg
      601 gacgcgacgt
//
LOCUS       DAIAHG010000196          584 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_196_length_584_cov_0.810651, whole genome shotgun sequence.
ACCESSION   DAIAHG010000196 DAIAHG010000000
VERSION     DAIAHG010000196.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196_length_584_cov_0.810651"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          203 a           60 c           93 g          228 t
ORIGIN      
        1 cttaaaatta ataaaatgta gtcaaattag gactcgattt gtaaatttgg acgaggaaat
       61 tcaaaataat gaacgaaatt tccatttttt tagcaattta tagagaaatc tacgaaaaaa
      121 tatttgattt tttcgaaaat tttcagtttt ttgggtgtat ttttcttaaa tttcggtaaa
      181 acttactcga aaacggatta gttttgaaga tatcagcgtg attatttcaa aatatgatca
      241 taaatttgca tttttacagc aattaatgga aaaatcagcg aaaaggtggt attttttcga
      301 aatgtttttg tttttttttg cttaaaatta atgaaatgta gtcaaatttg gactcaattt
      361 gtcaatctgg atgaggaaat tcaaattaat gtatgaaagt tccattgttt tagcaattta
      421 tggagaaatc tacgacaaaa tgggattttc ttcaaaaatt ttgaaaaact gaaaattttc
      481 gaaaaatggg attttttcga taattttcag ttttttgctt gtatttttct taaatttcgg
      541 taaaacttac tcgaaaacgg attccttttg aagatcttag cgtg
//
LOCUS       DAIAHG010000197          566 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_197_length_566_cov_2.208589, whole genome shotgun sequence.
ACCESSION   DAIAHG010000197 DAIAHG010000000
VERSION     DAIAHG010000197.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197_length_566_cov_2.208589"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          181 a           84 c          112 g          189 t
ORIGIN      
        1 agatatcatc ctttatggaa attggcctac tcagtcctta ttcttagggt gtggagttat
       61 tatagcatcg gggcttatga ttgtttttca ggaatctaaa aagagaaaag tatacgataa
      121 gggttgacct ctatttaaga tacttatatg ttgcaacaac ttagggcgct tagctcagtc
      181 ggtagagcat ctgactttta atcagagggt cgaaggttcg aatccttcag cgcccaccag
      241 gtaaatcaat agcttagaaa ggaggtaaaa aattacaaac cttctaggta agcacatggc
      301 aagcaaattt taaaaagtca taaaacttac atctttccta attcctcact aaattttttt
      361 ccaaggtcat ttaaaattaa aagagtggct aacttgattg gaagcccagg tgttaatttt
      421 gatgaaagta gcgtatgaat taaaaaggag gctgttgtat ggcgtgtcat caattgaata
      481 aagtttaagt ttctgtttta attggaaaaa tatttagaaa ggttttttca atgcgccata
      541 tattcattgc tttaggttac tttttt
//
LOCUS       DAIAHG010000198          566 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_198_length_566_cov_1.267894, whole genome shotgun sequence.
ACCESSION   DAIAHG010000198 DAIAHG010000000
VERSION     DAIAHG010000198.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198_length_566_cov_1.267894"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    285..294
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          156 a          134 c          113 g          153 t
ORIGIN      
        1 ccccctatgt cctgcattta gcagctgcta accttcttgt ataccctcaa ccctatgcct
       61 ttgaagcctt gcgggagaaa ttcagcctgg cgatcaaggg aagccccgaa gaaaaagtgt
      121 ttcttattct cacaacaccg gatcaaccga ttcaaaaagg gctcacacaa tttgaagaca
      181 ttgaacaact cattgcttat gcgaaatctc tttcataggt ttttgtgaaa agagcttgac
      241 cttttgggat catttcaggc aaaaataagg gtacgcgatg gatgnnnnnn nnnncgaaaa
      301 gagtagaatc tgcaagcact aggatagttt tttataaata tattttggat gggtggccga
      361 gcggttgaag gcaccggtct tgaaaaccgg caagggtgaa agcccttcgt gggttcgaat
      421 cccaccccat ccgccatttt cctaaaaaat tctagagcct tccttttcat gacttataat
      481 cttctgggat gctttttttg cgaaccccct taattcctca agcacgctca acacaagatc
      541 tgtccccccg taaaacatat catgag
//
LOCUS       DAIAHG010000199          526 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_199_length_526_cov_2.360802, whole genome shotgun sequence.
ACCESSION   DAIAHG010000199 DAIAHG010000000
VERSION     DAIAHG010000199.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199_length_526_cov_2.360802"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          173 a           95 c           99 g          159 t
ORIGIN      
        1 atgaggttgt caaatttatc acagacaagg acggtcaaaa gaaggctatc gccttaatgc
       61 tcattgtgct taaagggcgt cttaaaatgt ttgaaacacc tgagattaat cctaatatgc
      121 tgatcacaaa actgaaaaaa gaaaatgacc tgtggcttca aagttcacga aattatgcgg
      181 aaaaactaaa gcaataaggg cgtttcattc cttaaaatat cagggagaga cacccgttat
      241 aaactgttaa aaataaaaat tttttccttc tccttataaa ttctcttgca aaaaaatatg
      301 agcgagatat actccctaca taaattgcgc taagcgggcg tagctcaggg gtagagcaca
      361 accttgccaa ggttggggtc gagggttcaa atcccttcgc ccgctccatt ttttttctca
      421 atctttaatg tttcttaact ttttcgtgct taaatagata tagcgtggga tttttcccgt
      481 taattcttag gacatgatgt ctgttttgaa aaaaaagaaa gaattt
//
LOCUS       DAIAHG010000200          520 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_200_length_520_cov_2.548533, whole genome shotgun sequence.
ACCESSION   DAIAHG010000200 DAIAHG010000000
VERSION     DAIAHG010000200.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200_length_520_cov_2.548533"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          127 a          141 c          154 g           98 t
ORIGIN      
        1 taagcatata aacggccacg ccttcttccg tagggccgtc cgcaatgtgc cagatcaggg
       61 caaaacccgc gagacagccg atttcgagcc agaacagcgg ccgaacatgc ggcagccggc
      121 gcaagttggc cggatcagga tcgtacaacg ctgcggtcat tgccgctccg aaaatgaaac
      181 ccgcgaatgc aagcagagaa tgggcggctg tcagcatgtg gcccttgctg atggcgatgg
      241 ccagcaaggc aacgtttcct gtcatgcctg aagtgaacac ttcgcccagt ttcatgaatg
      301 caagcacgtc ggtgcaaccc gacgcgcaag acaggaagag gagaccaaac ccttctgtgg
      361 ttgtgatgct tttcatagcg caccggagtg ttgaattgcg ggcaacagca tgcgaaccag
      421 gctcccggga ttagcccagg gaaatttctg gcggagagag agggattcga accctcgata
      481 gaacttttgg tcctatgctc ccttagcagg ggagtgcctt
//
LOCUS       DAIAHG010000201          512 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_201_length_512_cov_2.340230, whole genome shotgun sequence.
ACCESSION   DAIAHG010000201 DAIAHG010000000
VERSION     DAIAHG010000201.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201_length_512_cov_2.340230"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          175 a           86 c           92 g          159 t
ORIGIN      
        1 tcctgcaaaa ttctaagcat gtttcaaaag aaaaatcaat cttaaagaag ttatgtagat
       61 ttgtgcatca tccataaaat gggtgatgtt ttttgggcaa tcgtgatctt ggatttcttt
      121 taactgcttc cgatatgctt ccgaagtgtt ttctgctagt tgacaacgag aggaaaaccc
      181 tttataaaga ctggtgccgg atgtcggaat cgaaccaacg acctactgat tacaaatcag
      241 ttgctctacc agctgagcta atccggctgt gtttaaggtt tataacgtat atggcattct
      301 aggccaagcc aaaaagaaat ttgagagcaa aagatatttt tgttaaatta taaccattta
      361 atggacggca ttttccaaac ttcattaaag actgcctctt tggaccaata aagtcataag
      421 attaaataaa aaattaattt actataaaaa ataaaggtat cccgttttat ggaaaatatt
      481 gagaccttaa gaaagcgctt gctctatcaa ag
//
LOCUS       DAIAHG010000202          505 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Holosporales bacterium isolate clean6003
            NODE_202_length_505_cov_1.418224, whole genome shotgun sequence.
ACCESSION   DAIAHG010000202 DAIAHG010000000
VERSION     DAIAHG010000202.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28413968
            Sequence Read Archive: SRR13304626
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holosporales bacterium (mine drainage metagenome)
  ORGANISM  Holosporales bacterium
            Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Holosporales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202_length_505_cov_1.418224"
                     /isolate="clean6003"
                     /isolation_source="sediment"
                     /db_xref="taxon:2053559"
                     /environmental_sample
                     /country="China: Anhui, Wuhu"
                     /lat_lon="31.08301 N 118.25624 E"
                     /collection_date="2016-10-30"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          113 a          122 c          127 g          143 t
ORIGIN      
        1 gcatgatgag tgttgctgcg cgtgcatcca tactgtcgat tgtatgacca aagtatgacc
       61 acgacaatat ttatctgttt tacatatcta ttatcaatag tatacaaact aacatattcg
      121 aatccctctc tctccgccag caacccttcc cagaacatcc aagaatatcc aagattccca
      181 agaaataccc agtaaatacg gggtctttgt tttttctact tctatcgcag tgcgatgaaa
      241 gcctgatgcc gctggggtat ttctggggta gcttcccggc gaactagggg tattttcccg
      301 gaaggccact tcggaggctg aatggtggtt cgaatggagt tctccggagt tccgttggtt
      361 gtgagtttgt gaatgcgttt cgtcattgca tccgacttcg ggcaatcaga atggcatagc
      421 ccagcttgcc gctgcgtatg gcttcggccg cagcgcgggc gaactgtttg gccgcgctga
      481 gatcaagtcc ggggatgtcc agttt
//
