LOCUS       DAIANL010000001         6499 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_505, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000001 DAIANL010000000
VERSION     DAIANL010000001.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1311 a         2228 c         1841 g         1119 t
ORIGIN      
        1 ggcctatgac atccgcggta tcgttggcaa gacgctgacc gcagacatcg tcgaacgcat
       61 cggacatgcc attggttcgg aagcgatcgc ccgcaaccag actcacatct gcatcggccg
      121 tgacggcagg ctgtcaggac cggaactcgc tgcggcactg gcacgtggca tccgcaaggc
      181 gggcatccat gtcatcgatc tgggcatggt tgccaccccg atgacctatt tcgccgcctg
      241 gcaactcaag accaattcgg cggtcatggt caccggctcg cacaatccgc ccgattacaa
      301 cggcctgaaa atggtgctgg gtggagaaac cctgtccggc gacaccatcc aggcgctacg
      361 cgttcgcctc gaaaccggcg acctggccca cggcgaaggc cagtacagcc agcacgacat
      421 cgtgaacgaa tacatggacc gcatcgtgtc cgacgtgaag ctggcacgcg gcatgaaaat
      481 catcatcgat gccggcaatg gcattccggg cgcattcgcc cccacactct accgtcgtct
      541 cggctgcgag gtggaagaac tgttctgcga agtcgatggt cagttcccca accatcaccc
      601 cgatccctcc cagcccaaga acctggttga cctgatcgcc cgcctcaaga gcagcgacgc
      661 cgaaatcgga ctggcattcg atggcgatgg cgaccggctg ggcgtggtaa cgaaaaatgg
      721 ccagatcatt ttccccgacc gccaactgat gctgtttgcc gacgacgtgc tgtcgcgcaa
      781 cccgggtgcg gaaatcatct tcgacgtcaa atccacacgc aacctgtttg actggatccg
      841 cgcacgcggt ggccgtccgt tactgtggaa aaccggtcac agcttcatca aggccaagat
      901 gaaggaaacc ggcgccctgc tcgccggcga gatgtccgga cacattttct tcaaggaacg
      961 ctggtatgga ttcgacgacg gcatgtatgc cggcgcccgg ctgctcgaat acctgagccg
     1021 ccaggctgat ctcaccgcca cactcaacag cctgcccgac acggtgaata cccccgaact
     1081 caacgtcagc ctgcctgaag gtgaacattt ccgcctcatg gcccgtctgc aggaaagcgc
     1141 ccggttcccc gatgcccttg atatcatcaa gctcgacggc ctgcgcgtcg aatacgccga
     1201 cggtttcgga ttggcacgcc cgtccaatac cactccggtg atcgtgctgc gtttcgaggc
     1261 caatacccaa actggtctgg aacgcattca ggccgatttt cgccgcgtat ttcacgaagc
     1321 cgcccctgga ctggcgctgc cgttctgacg caaaactgac aaatcggcgg aggaatgccc
     1381 atggttgtag catatttgca acaattgtac agacttgagc atccgcgcac cattacaccc
     1441 atccgccacc ttgagctggt gctagaatga gccggtctgc gccgcatgta cataaaacga
     1501 ggcgccgtct gcgacccacg taattcatat ccaggaggaa acaatgtccc gctcactacg
     1561 ccagatcatt gcactgtcct gtgtactggc tgccggccag gcacttgccg acagttccag
     1621 ccagcaagac caacgtttct atttcagccc cagcgccacc tacaactgga tggacagcaa
     1681 ctggggagtc aacaacggca tgggcctcaa ccttggtatc ggcaagccgg tgtccgagca
     1741 ctggaacatt gaaggcaacc tgtcatacac cgaactgggt taccagaacg gcaacaacag
     1801 cctcgacaac accgacctca acgtcgatgc catgtatttc ttcaaccgca acccctcctt
     1861 ctcgcccttc gtcgagatcg gtgcaggcgg cctgtacgct cagggacgca gcaactccag
     1921 cacctatggc gacgccaacg ccggcgtggg tttcatgacc tggctgcatg acatcgccat
     1981 tcgtgccgac gtgcgctacc gctacaccgg ctccgtggct gcctgggcag cgaacgtgcc
     2041 gggcagcagc ttcagccccg acgactggat tgccagcctc ggtctggtga ttccgctcgg
     2101 cgaaaaagcc aagccggcac cgatcgcgga agctccggct ccggctccgg cccccgcacc
     2161 agctcccgca gcagccccag aaccggctcc tgcacctgct ccggcccctg ttgaggctgt
     2221 gccgccgcgc ccggtcgcac acaccaagct ggtgctcgaa ggctcgcact tcgccttcaa
     2281 caaggccacg ctggatgctt cgggcaaggc caagctggac gaggatgcca aactgctggt
     2341 ggcttatccc gacatcaagg tcaagatcag cggctatacc gacagcatcg gcagcgtcaa
     2401 gtacaacaag cgcctgtctg accgccgtgc cgacaccgtg ttccgctacc tgcagagcca
     2461 tggtgtgaaa tccgatcgca tgaccatgca gggttatggc gagtccaacc cggtcgccag
     2521 caacaagacg gccgcaggtc gcgcaaagaa ccgtcgcgtc gagatcgaaa ccctcaactg
     2581 agcggcgttg caccctctga aaaaccgcgc tttcgggcgc ggtttttttt cgtccgactg
     2641 accggagtgg gcaaacatgg cactcagccc gccccaccca tgtcctgcca tgccttttcg
     2701 agacgcttga acgatacggg ctgcgccgtc ttgagttgct gggcaaacag cgagacgcgc
     2761 aattcttcca tcagccagcg aaaggcgtcc atgcgtggat cctgctcgcc cgccttgcgc
     2821 aaagcagctt cgcgtgcctg ccagatggcc gtccaccgcg ccagaacggc gacatggcgc
     2881 tcgtcctggg ccttgcgttc aggcagtttg tgcaaacgca gttgcaacgc ctgcaggtaa
     2941 cgcgggaaat gctgcaacgc gttccatggc gtggtacgca agaacccggg gtagatcagc
     3001 gcgcgcaact gctgctgcat gtccttgccg gacggaccgg tgacctgggc aatctgcgca
     3061 gtcaatgcct gatacagcgg caggatctgg cccagggtgc gcatcagcgc atcggccacc
     3121 gggcgcagcc gcacacgcgc cctgaccagc agcgcatcga atgcagcgcc gttgcgtggc
     3181 ggcgtctcat cgcccagaca ggcctgcgcg gcgatcacct ccagcgccat ttcgcgcaac
     3241 gaatcggaat cacccaatgg ccgccacgcc agtgcgaccc gggtaaactc gggcaaatcc
     3301 ttttccagcg ccctgaattg cgccgccagc gtcagtcgga acagccgcaa cacgccctgg
     3361 cggtgagcgt ccgccgccgc ctcgcgggta tcgaagcgtc gcaccgcgac gctgtcaccc
     3421 tcatcgacga cggccggcca ggcagtcacc tgcacgccag cccgcatcac cggcaacgat
     3481 tcgggcacat cgccgaattc ccaggcacgg aagcccgcgc tctcggccgt agcctccgca
     3541 tcggcaaaca ccatctgcgc agcctgcccc aactgggcac gcagcgcagc cagatcgcgc
     3601 cccgcggcca attcctcacc ctgggcatcc agcacgcgaa aattcatgcg caaatgttcg
     3661 ggtgccggtt ccttccagtc ggcagcagtc accgtcacgc cgccccgcag cgtcagccag
     3721 gccgcaagct gcgcggccag cggcccttcc actgcaatcg cttccatcgc cgcggtcacc
     3781 gtatccggca ccggcacgaa cagccgtcgc agtggcttcg gcagattccg gacataccag
     3841 gtgatctttt cgcgcaacat gcccggcacc agccagtcga aacgccagcc aggcagggca
     3901 ttgagcagcg acaggggcac gtctgccgtc accccatcca gcggatgtcc aggctcaaaa
     3961 cggtaatgca gcgccagtgc cacgccatcg agctgcaact gctgcggaaa ccatgcctcg
     4021 gtcacggtct gcgccccgtg gcgcatgata tcttcacggc gcaggaacag cagatgtggc
     4081 tgaccgcgtt cggcctcgcg ccgccagcgt tcgaatccgg ccccattgac gatgtctgac
     4141 ggcaggcgta catcgaagaa atcgtacagc gcctgctcgt cgatcagcac atccgggcgg
     4201 cgcgccttgt gttccagttc ctcgatgtca gcgatcagtt gccggttata tgcaaagaat
     4261 ggcgcctgcg tgtcatactc accggccacc agtgctgccc ggatgaaaat ctcgcggctg
     4321 gctttggcgt cgatcccacc gtaatgcacc ttgcgtcggg gaatcacaat caatccgaag
     4381 cattgcacct gttcccaggc attcacctgg gcggcgcgtc gttcccagtg cggatcgaaa
     4441 taatggtgcc ggcacaggtg ggctgccacc cgctccaccc attccggctc gatacgcgcg
     4501 gccatgcggc catacaagcg cgtggtttcc accagttcgg ccaccatcac ccacttgggt
     4561 ggctttcgcg ccaatgtcga cgatggatgc agatgaaagc gtatgccgcg cgccccctgg
     4621 aacccccctt cgtcctgctt gacgccgata tttccaggaa accaggacag cagcgccagg
     4681 tgcacggcct cgtaggccgc cggctcgacg ttttcgcgca aacccagttc agctgcgtgc
     4741 accgccagtt gcccgtgaat ttcgcgccac tcgcgcaaac gcgccggatt gagaaaatgc
     4801 tcctgacaca acgcttgcag ttgccgccgc gatttctggt gccgcaacgc gtcctcataa
     4861 aatgcccaca gcttgagcaa ccccatgaaa tcggagcgtt cgtcgacaaa ggcccggtgg
     4921 cgatcgtcgg cagcctgcgc cttgtcagcc ggccgctcac gcacatcctg caccgacagc
     4981 gccgcgcaga tgaccagcgc ctcgcgcaga caaccgtggc gctgcgctgc aagcagcacc
     5041 cggccgatac gcggttcgag cggcagccgc gccagcctgc gtccgacatc ggtcatgcgc
     5101 cggcgctcat cgaccgcacc cagttcctcg agcaactgat agccatcggc gaccgcgcgc
     5161 ggcgccggtg catcgaggaa cggaaactcc gccacatcgc ccagtcgcaa cgacagcatg
     5221 cgcaggatca ccccggcgag tgaagtccgc agaatctccg gatcggtatg cgcaggacga
     5281 ccggaaaatt ccagttcgga atacaggcgg atgcaaatac cggccgccac ccggccacaa
     5341 cgtcccgcgc gctggttggc cgatgcctgc gacaccggct ctgtcagcaa ctggtcgatc
     5401 ttgttacgcg tcgaataccg caggatacgc gcggttcccg tatcaatcac atgacggata
     5461 cccggcacag tgagggaggt ttccgccaca ttggtggaca gcacgatccg tcgcgcgccg
     5521 cccggatgaa agatgcgttc ctgctcgcgc accgacatgc gcgcaaacag tggcaggatc
     5581 tcggtgtgca ccggatggtg tttgcgcaac gcctcggcag cgtcacggat ttcgcgttca
     5641 cccggcaaaa acaccagtac atcgccatcg ctctcatgcg ccagttcatc gacggcctcc
     5701 agcaatgcaa gcgtcgcacg ttcctcgtcg gtcggttccg tcgctcgccg ccctggaaca
     5761 gccccgtcca cggcgcgctc gggcgcctgt atcggccgat agcggatttc caccgggaaa
     5821 gtacgcccgg acacctcgat cactggcgca ccgccgaaat gacgggaaaa ccgttcggcg
     5881 tcgatggtgg cagaagcaat cacgacacgc aggtccgcac gctgttcaag caggcggtgc
     5941 aggtaaccca gcaggaaatc gatgttcaga ctgcgttcgt gagcctcatc gagaatcacg
     6001 gtatcgtatt cacgcaactc gcgatcgcca gcggtttcag ccagcagcat gccatcagtc
     6061 agcaccttga cggcgctgtc ggcacgcacc tgatcggaga aacgcacctt gtaacctacg
     6121 ccttcgccca cccgcgtatg caattcttca gcgatgcgcg cagccagcgt tcgcgcggcg
     6181 atacggcgcg gctgggtaca ggcgatgcgg ccggtgcggc cacgtccggc ttcaagacac
     6241 agcttgggca actgggtcgt cttgcccgac ccggtagcgc cgctgatcac cacgaccgga
     6301 tgctcacgca gcgcagtgac gatttcggtg cgcatcgcca ccaccggcag gtgctccgga
     6361 tagtcgagcc gctccggtaa ccacagaccg gtcagcgccg ccgtggatgc ggctgccgga
     6421 cgaggcgccg cccgcttcat ttgccgttga tgtacatctt gtcgtcgaag gtcgccaccc
     6481 attgcggccg gtacaccac
//
LOCUS       DAIANL010000002         9611 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3066164, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000002 DAIANL010000000
VERSION     DAIANL010000002.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3066164"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1833 a         3235 c         2775 g         1768 t
ORIGIN      
        1 gattctcgtt ggacgccagc ttgacgatac gtgccgggtc caaccccatt tcccgggcca
       61 gttcggcaat cggcttgcct ggctggtacg gagcaatggc ccgcacgtgc gccggagaca
      121 ggtcacaaca cgaattcaac atacgcttcc cggataagat cccagcactt tcatcaggct
      181 ggcctgtgcg gatacttcct gcaatgccgc cttgaccgcc gcatcctcgc gatggccttc
      241 gatgtcgacg aaaaaaacgt attcccagat cggggtgccg cggctgaacc cgccgagtcg
      301 gctgcgagcc ggacgcgatt caagcttggt cagcgaaatg ccatgcgtgc tgaacggctg
      361 cagcaggctc acgatcgccc ccggctcgtt gggacgcgac agcagcaacg aggtcttgtc
      421 attgcccgac ggcgcggcga cgtcgcgtgt ggcaatcacc acgaagcggg tggtattgtc
      481 cggctcatcc tcgatgcggt catgcaaccg gtacaggtgg taatgctgcg ccgcagtctc
      541 tcccgccacc gccagtgcgc tgctgtcttc cgcggccagg cgtgccgctt cggcgttact
      601 tgccaccgcc acctgtgaca cgccgggcag atggattgcc agccagttgc ggcactgggc
      661 gagcgattgt gcatgcgaat agatcacacg cacaccttcg aaaccttcca ccttgcgcag
      721 cagtgaatgg tggatgcgca acgcgacttc gccactcaga tgcaacggtg tttcctgcag
      781 caggtcgagc gtacggttca ctgcgccttc ggttgagttc tccaccggga cgataccgta
      841 gtccgccgaa ccggcttcga cttcgcgaaa cacctcgtcg atcgacgcac acggctgcag
      901 ttgcaccgca tggccaaact ggcggagcgc agccacttcg ctgaatgagc cttgcgggcc
      961 gagataagcc accgtcagcg gccgttccag cgcaaggcag gcagacatga tctcgcgcca
     1021 gacgcgcacc actgcctcgc gccccatcgg cccacggttg tgttccgcca cgcggcgcag
     1081 tacctgcgcc tcgcgttctg cgcgatagac cacaccgttt ttcagtttgc cgaccgcctg
     1141 ggcgagcgca gcacgacggt tgagcaattc gagcatatcg atatcgatcg tgtcgatctc
     1201 gcgccgcaat tcggccagcc gcgccgtgtc ttcctgtgta atgtcctgcg atgccatgtc
     1261 atccatgctg acgaacaaat tccttcatgt aattgaccag cgcttccacg ccggccagcg
     1321 gcatcgcatt gtagatcgac gcacgcatac cgccagccag ccggtgtccc ttgagctgga
     1381 gcagtccatt gcgttcggcg ccctgcagaa aagtggcatc gagcgcggga tcggtcaggg
     1441 tgaatggcac attcatgagc gagcggctgc cgggctcgac cggattgcgg tagaaaccgc
     1501 tgccatcgat ataaccatac agcagttcgg ccttggcgcg gttgcgctcc gccactgccg
     1561 gcaagccgcc gtcgcgcagc aaccgcgcaa acaccagccc ggccatgtag atcgcccagg
     1621 tcggcggcgt gttgaacatc gagtcatgct gtgcatgagt acggtagtcg agcatcgtcg
     1681 gcgtgcctgg cattggtgtt ccaatcaggt cttcgcgcac gatcaccagt gtcagtccgg
     1741 ccggaccgat gttcttctgt gcaccggcgt aaatgaggcc gaaccgcgac acatcgaccg
     1801 gttgcgacag gatgtccgac gacatgtcgg caaccagcgg tacagtgccg gtttgaggaa
     1861 tccagtgaaa ctgtaccccg ccaatggtct cgttggtggt gtaatgcaga taggctgccc
     1921 cgggatccat ctgccagccg gattgcggcg gaacggcatg aaacccggat gattcgctgc
     1981 ttgccgccag gcgcaccctg ccgaaacgac cggcctcttc gatggctttt tccgaccaga
     2041 ttccggtgtg cacgtaatcc gcacccggac ggccacgcag caggttcatc ggcaccatgg
     2101 caaattgcgt ccacgcacca ccctgcagga acagcacacg ataatcggac ggaatgccaa
     2161 gcaaggcgcg caaatcggct tcggcctgat gctggatgcc aaggtacggc tcggcgcggt
     2221 ggctcatttc catcaccgac atgccactgc cgtgccagtc gagcatttct tcacccgctt
     2281 gcgcgagcac ctcttcaggc agaatcgccg ggccggcgct gaaattgaat acccgcatcg
     2341 aaatcgtccc gtcaggcttt gtgattgccg gcaatcagcg cctgcccgat gggcagcact
     2401 tccgcttcgg tcacgccggt gttgctgtaa ccgacgaaac gcgcgccatt ttcgatttcg
     2461 agcgccgccg ccgagacatc accttcaacc agcgcctgac gatggatgcg cagcagaccg
     2521 cgtgcactga tacaactttt cacctgacca tgcaccacca cgttttccgc actgagggta
     2581 tcacccacca tgcgaccgct gccggcaatc tccacgtcac ccttgacgat gaggttgccc
     2641 cgcacttcac cttccaggcg caggttgccc gggcaatcga cattaccgtc gatgacacag
     2701 ccgctgccaa tgaaggtcag gttcgcattc agttgcagtc cggacgattt atgatccgat
     2761 ttcgatgaag atttgaacat gacaggactc ctattcgaca tagggataag ggtcgatcag
     2821 cacgccattc agatagaccg cgtaatgcag gtgcggtccg gtagaacggc cggtattgcc
     2881 cgacaggcca atgacctgcc cggcttgcac ggcttcaccg acatgcacgc gaatctgcga
     2941 caggtgtgca taggccgtac ggtaaccata accgtgatcc accaccacca gattgccatt
     3001 cggcccggac aggccggcgg tgatcaccgt gcccggcgcc gtgctccata ccggggacgc
     3061 aaaaggagca gcaaaatcca gccccttgtg gaattcataa ctgtcttcgc caaacgggtt
     3121 ggcgcgtaca ccgaagcgcg aggtgatgtc ggcagtacgg gtaatcggcc agccttccgg
     3181 acgcgcctgc aactgcgcga gcgccacgct cagctcgccg atacgcgact gcacctgttg
     3241 ctgcatcgcg gaaaacgccg cttcacgact catcggccag gccaccccac ccatcgccgc
     3301 tggcgctacg gtttgactgc ggccaaccgc ggctgccggt tcatggtaac ggtcggcaag
     3361 tttgcgcacc tggttttcga gcatgcccag ctgatcgagc atcgtctggt aacgtgtggc
     3421 cagcacgctg cggctatggt tcagcgccga gttgtctgcc tgcagcacct tcatctgttg
     3481 ctcgaccaca tcggcatggt ccagcgcctg actgttgacg gcatggtacc atgccgccca
     3541 acccagggtg acgagccata acgtcagcat gattccgaac gccgtcagcg cccagtgcag
     3601 ataatacaga ttgaaacgca acgaaaaggc ggccttgccc gtgcgcagga tgatcagctc
     3661 gacatggcgc gggtggcgac tttccgcccc gtccagtcca agccagtcac ggatcgccgc
     3721 ccaccgcatc atgcgtcatc atccttttcc agcacgcgtt ccacgctgat gagtttctgc
     3781 ccctgatcga gattgatcag tgtcacgccc tgagtggcac gacccagttc gcgcacttcg
     3841 cgcacacgcg tgcggatcag taccccaccg tcggtaatga gcatgatctc gtcatcggga
     3901 tcgaccagtg tggccgcgac cacctggccg ttacgcttgg tggtctgaat ggcaatcatt
     3961 ccctgtgtcc cgcgggcatg acgcgtgtat tcggctacgc tggtgcgttt gccgaagccg
     4021 ttttccgtgg cggtgagcac cgattgctgt tcgttttccg caaccagcag cgaaatgact
     4081 ttctggtcag cggcaagctt catgccgcgc acgccacgtg cgccacgtcc catcgcgcgc
     4141 acgtcatttt catcgaaacg cacggccttg ccggcgttgg aaaacagcat gacgtcgtgt
     4201 tttccgtcag tgagcgccac accgaccagg tagtcatcct tgtcgagccc caccgcgata
     4261 atgccggcac ggcgcggatt ggaaaattcg gacagcgcgg ttttcttcac gacgccgcac
     4321 gaagtcgcca tgaaaatgta gcggctgtcg acgaattcgc gtaccggcag aatagcgttg
     4381 atcttctcgt tttccgccag cggcagcaga ttgacgatcg gcttgccgcg cgcagtgcgg
     4441 ctaccctgcg gcacttcata caccttgagc cagtacaccc gccccatgtt ggaaaagcac
     4501 aggatgtaat cgtggctgtt ggcgatgaac aggcgatcga tgaaatcgtc atccttggtc
     4561 ggcatggcct gcttgccgcg cccgccgcgc ttctgcgcgt gataatcgtc gagcggctgc
     4621 gatttcatgt agccactgtg cgacagcgtt accaccatat cctgcggcgt gatcagatct
     4681 tccaggctca ggtcctgggc gttggacacg atttcggagc gtcgcgcatc gccgaactgc
     4741 tggcggatct ggccgatttc gtcgcgtatg atctgtgtca cccgttccgg acgggcgaga
     4801 atgtcgagca gatcggcaat cttcgccacg atctccttgt attcgcccgt gattttttcc
     4861 tgttccagcc ctgtcagacg ttgcaagcgc aggtcgagga tcgcctgggc ctggacttcc
     4921 gacaagcggt agccaccttc gacgaaaccg aaactggcgt ccacgccatc cgggcggaac
     4981 tggctggcat cggtcatcgc ccgcgccagc atgtcttcca ccagcgccga acgccacgca
     5041 cgcgccatca gtgactgacg ggcatcggga ggtgtgggcg cagccttgat cagcgcgatg
     5101 atttcgtcca cattcgacag cgccaccgca aggccttcca gcagatgccc ccgctcacgc
     5161 gccttgcgca gctcgaaacg cacgcgccgg gtgacgactt cgcggcggtg ccgcagaaag
     5221 gcttccagca tctgtttcag gttgagcagg cgcggctggc cgtcgaccag cgccaccatg
     5281 ttcatgccga agctgtcctg catctgggtg tgcttgaaca ggttgttgag caccacttcc
     5341 ggtacttcgc cacggcgcaa ctcgatgacc acgcgcatac cggaacggtc agattcgtcc
     5401 cgcagatccg aaatgccttc gatgcgtttg tcgcggacca gttcgccaat cttgatcagc
     5461 aggttggcct tgttcacctg gtagggcaac tcgtcaatga tgatcgcctg acggttaccg
     5521 ccgcggtcca gatcttcgaa atgggtgcgc gcgcggatca ccacccggcc acgaccggtc
     5581 agatagccct ggcgcacgcc gaccagaccg tagatgatgc ccgcggtcgg aaaatccgga
     5641 gcctgcacca catcgatcag cgcctcaaga ctggtattcg gatcgtcgag cagcgccagg
     5701 ctcgcctcgc acacatcgcc cagattgtgc ggcgggatgt tggtggccat gccgacagcg
     5761 atgccggccg aaccgttgat gagcaaattg ggaatcctgg ccggcaggat cagcggttcg
     5821 cgttcggacc catcgtagtt ggggccgaaa tcgaccgttt ccttgtcaat gtccgccagc
     5881 agttcatgcg caatgcgtga catgcggatt tcggtataac gcatcgccgc cgggttgtca
     5941 ccgtcgaccg aaccgaaatt gccctgcccg tccaccagtg ggtaacgcag cgaaaaatcc
     6001 tgcgccatgc gcacgatggt atcgtacacc gcagtgtcgc cgtgcggatg gtatttaccg
     6061 atcacatcgc cgacgatacg cgaagatttc ttgtacgccc ggttccagtc attcgacatc
     6121 tcgtgcatgg caaacagcac gcggcgatgc accggcttga gcccgtcgcg cacgtcaggc
     6181 agtgcacgcc cgacaatcac gctcatggca taatcgagat aggaacggcg catctcttct
     6241 tcgagactga ctggcagggt ttccttggca aacaattcca tgatacagcg accaaatcag
     6301 gacagacgaa cgccggattc tagcacaccg caaacaggct gggcaggggg actgacgtcg
     6361 accgctcccc ggcacattgc ggcctgcggc cggccagcgc agccatgatc cgcagtcagg
     6421 cacacgccct gctcatggaa cgtctgcgcc tcgaaccgca acgcgccgac gactggctgc
     6481 tgctggcgcg cctgctcggc cgtgatggcc acagcacagc agcccgtacg gcctatgcag
     6541 ccgcactgca ccaccactgc gcggaacccg tgcaggcgca tctcgaactg gccaacctgt
     6601 tgctcgatgc cggcgacgcc gcaacggccg cgacacacta ccgcaccgcg ctcgcgcttg
     6661 aaccagacaa tgcggccgca caccaggggc tcagtgccgc ctgcgacctg cttggcgata
     6721 caaccgccgc cgaacttcac cgccgtaccg gtttcggcgc acagccggta atccggctcc
     6781 ccgctgcagc acccgacgcc acaccgctga tcgtgctcgc ctcggcatgg catggcaatc
     6841 tgccctggcg gcgactggtg gacacacacc gcttccaggt cacgatcgtc tgcgtcgaat
     6901 tcctgccggc aacattcacc ctgccgcgac acaccccggt actcaacgcc atcggcgatg
     6961 ccgacctgtg ccgcgacggc ctgctgcgtg cgcagcaact ctgcgccgcg cagactcagg
     7021 tgctcaacct gccgcagcac gtgctgcgta ccggacgcgc ggacacagcc cgccgactga
     7081 gcgacattgg ccacgtacgc accgcacctg ttacccgcct gcaccgctcc cagaccacgc
     7141 ccaacgcctg gccggacacc ctgccgcgcg agcgcagctg gctggtgcga gccccaggct
     7201 tccagggagg cgagcatttc atccgcatcg acccggacac cgacacattc acggcgcttg
     7261 ccggtctgcc tggcgaccac tggctggtca tgccgttcat cgacacgcgc tgcaacgatg
     7321 gcctgtaccg caaatatcgc atgctgagta tcggcggccg actctaccca ctgcatctgg
     7381 cagtttcgac gcactggaag gtacattact tcagcgcgac cgcccgcacc gatcaggcga
     7441 tctgcgccga ggaggcgcgt ttcctcaccg accccgctgc agtgctgccc cctcacgcgc
     7501 tcctggcgct gcacgcgatc cagcacatgc aggcactgga ctactgcggt atcgatttcg
     7561 ccctggacgc ggacggcgaa ctggtggtat tcgagagcaa tgccaccatg gccatcaatc
     7621 ctccgcccga cgaggccgcg gacaacccgc gcgccatcgc tgcccggcac gccctgcagg
     7681 cggcgcacga cctgctcgac aacgcggcct gccgcgcact ggacagcaac gatgaacagt
     7741 gaaatgattg gaatgtccgc tttaccgcca gagcgccccg ggaggtgcca tgcatgtgct
     7801 cgtctacagc agtcgcccgc acgatcgcga agtgctcgaa cctgcactgg tcgcagccgg
     7861 acaccagatc agccacaccg aggccacgct cgacgagctg aacctcgcgc tggcgcaagg
     7921 gtgcgatgcc atctgcacct tcgtcaacga cgaactcgat gctgcgcgcc tgcaacgtct
     7981 ggccgacatg cgggtgcggc tgatactgca gcgggccgcc ggcatcgaca acattgacct
     8041 cgacgccgcc cggcgcagcg gcatcgccgt agcgcatgcc ccagcgtatt cgccccatgc
     8101 cgtggccgaa cacgccgttg ccctgctgct gtcgctcaac cggcatgtgc atatcgcctg
     8161 gcaacgcgtg cagcaaggca atttcctcct cgatggtctg ctggggttcg atctgcacgg
     8221 caaaaccgcc ggcatcgtcg gcatgggacg gatcggccag tgtttcgcac gcatcatgct
     8281 cggttttggc tgccgtgtac tggccttcga tccgggcatt ccggaagacg cccgcatgcc
     8341 gggcgtcgaa tacgatacgc tcgacgccat atggcgcgca tccgacatcc tgtcgctgca
     8401 ctgtccgtta ctgcccgcca cgcgccacct ggtcgacgcg cgcctgctga cgcagtcgcg
     8461 cccgcatgcg ttgctcatca acaccagccg cggcgcggta gtcgacacgc gcgccgtgct
     8521 tgacgccctg agcgcaggcc ggctgggcgg ttacgcggcg gatgtgtatg aaagcgagcg
     8581 caacgtgttt ttccacgact gttcgggcaa ggaactcggc gacgcgctgc tgtgcgacct
     8641 gatccgccat cccaacgtgc tggtcacccc acaccaggcg tttttcaccc gcgaagccct
     8701 gcaaggcatc gcgcgtgcca tggtcgacag cctggcgcac tgggccgcag gcacacccgg
     8761 gacaggcttc gtcaccgccc gcccctgagc catgggtgcg accggctacg ccgggtccag
     8821 ccagccagcc agcgccggag ggatcttgcg cggagcacga atgtgcaaca ccttgtggac
     8881 actggtttgc cctcgcacca cttcgaccgc cgccgccggc acgccaaact ggacagcaag
     8941 aaacttcacc atcgcctcgg tggccttgcc gtgctcgggc gcggccgcaa tactgatttt
     9001 cagttcattg ccgcgcggct tgccaatggc atcacgcgag gcgcccggcg atccgagcac
     9061 gtacagcacc agcgtttctc cgtcccagcg aaaaaaacca gctgccgccc cgctcatgat
     9121 tacaccagcg ccagaatgac gctggccgcg ttgaacatgg cgcatacagg catgtctgcc
     9181 tgcaggccca tccgggtcgc cacggtggtg ttcacagcag cggtaatggt cttttcgttt
     9241 gccagccgca cactcacctc gctgtagacc accccatgct gcagctctcc aaccgtgcca
     9301 cacaaacggt tctcgaaact gatgggcgcg acaggatcga tacccagcac cacagcgcct
     9361 gccttgaaca gcgcgtacac gtccatgccc tcggccagcc gcatgctgtc catgctggta
     9421 cgcgtcacca ctgcagccag ttcggtgtca gcgtcgatgc gcacgcggac atcgacatgc
     9481 accggcccgg catggaggcg ggcgatgcgg ccggcaaact ggttgcgcgc actggtcgtc
     9541 agggaaaaac ggcgcagcag gcgatgaaat tcatcaaaat cctcgctatc accggccact
     9601 gcacccaacg c
//
LOCUS       DAIANL010000003         5706 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1432901, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000003 DAIANL010000000
VERSION     DAIANL010000003.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432901"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1103 a         1745 c         1707 g         1151 t
ORIGIN      
        1 gaacttaaca agtacagtat gcagctcggc accttcgtgg tgcaacctgc gttatcgagg
       61 ctggccacct gctaccggct ggcgaccatg atgtctagag ccacagactg gctgtcaacc
      121 cccccccttc gcgtgctgcc agccgtatcg tccagttatg cagcgtggcc agctggcggg
      181 caatggccag cccgagccca ctgccgccag tcgccagatt gcgcgactgc tccaaccggt
      241 aaaacggacg gaacaccgct tcgagctggt ccgcagcgat gcccgggcct cgatccatga
      301 catcgatgcg cagccgatcc ccttccagcc ggcagctgat ggtcacttcg gcaggcgcag
      361 ggcccgtgcg tccgtaacgc gtggcgtttt ccaccagatt ggccacgatg cgctgcagcg
      421 cctgctgcgc cacccggcga acacacggct cggcgggcca gtcggcatgg acgtggccgc
      481 cgcctcgacg cgcatcctcg agtacgcgct ccagcactgt acgaatgtcg accggctgcg
      541 cgacttcgtg ctgcatgcca cgcgccagat cgagaaagct gccgatcatc tgattcatct
      601 gttcgagatc gccttcgatc tgacgtacaa gtcgcgtgtc cgtttcgggt ggcagcatgg
      661 ccaccgccag gcgcatgcgc gcaatcggtg tgcgcaggtc gtgggaaatc cccgccagca
      721 gcgtggtgcg attggccaac agatcctgca cttcggtcgc catgcggttg aacgcctgtc
      781 cgagcgtcgc cagttcgctt gcgccgtctt caggcaacgg atggggaacg tgtccgcgac
      841 ccacttctgc tgcggccaga ctcatctgtg tcagccgcat ggcgatgcgt cgtaccagca
      901 gcaacgatgt cgcccaggtc agcagcgcag ccaggctgat cagaccgatg gtcgtgagca
      961 gcgcgcgaat atagtagcgt cgttcctcga acgacacgcg aatcaggtgc ccgcccatcc
     1021 acagatcggc ccacacccag ccggctttac cctgttgcag ctggatgtgc tggccggtac
     1081 gtgcagacag cgcgcgctcg atgttgtcgc tgttactgaa atgcgcgagc gggctgtcat
     1141 cgggtcggga cagcttgtga atgagcaggt gatggtgttc gctgagctcc tcctcgaatg
     1201 ccggacgcgt gccgggcggc aattcggccc aggtttgcgc cgaaagcacg atcagtgccg
     1261 ccaggtcatc ggccgagcgc cgtgccagcg gcgcaaagat caccacaaac accagcgtga
     1321 gcacgatggc ctgtaacagg aaaaaagcga tgcccagcgt cgccgcggta cggccataca
     1381 gggaacgcgg ccacaactgg attcgcaggc gatgcatctc gcgttatcct gttcaggctg
     1441 caacgctcac gaatgcgccc ccttgggcgc aaacaggtat ccctgtcccc acacggtacg
     1501 gatgtattgc ggttcggcgg ggttgacttc gatcttgcgc cgcaagcggg tgacgcgaac
     1561 gtcgatgctg cggtcgaatg gatcacggtc ataccctttg agcatatcca tgagttgatc
     1621 gcgcgacagt acgcggttgg gatgcgtgac aaaaatctgc aggattgaaa attcggctga
     1681 agtcagcgga atcgccaccc cttcgcgcgc cagcgcctgg gcgtcgagat ccagctcgaa
     1741 ttcgccgaag cgcaccttgc gcagggtctg tgccgactgt tgcactgtgg attcaccctg
     1801 tccgcgccgc agtacggccc gaatccgcgc cagcaactcg cgcggattga acggtttggc
     1861 cagatagtcg tctgcgccaa cttcgagacc aatgatgcgg tcaatgtctt cgccacgcgc
     1921 cgacaacatg atgatcggct gacggctgcc ggagcgcagg cgacgggcaa tcgacagtcc
     1981 gtcctcgccc gccatcatca ggtccaggat caccaggtcg aacgcctgct cggccatgcg
     2041 cgcatccatg gctgcgccgc catcggccag cgtcaccaga tagccattgc tgccgagata
     2101 ctggctgagc aactcgcgca gcccttcgtc gtcatcgacc accagtatcc gtgcccggga
     2161 tgattcttcc atcgcccacc ttcctgctac cattcgtgcg caacagtcta gcatgccttg
     2221 accggctcgc gcctgcaccc gccgccaggc atggccggac ggcaggctgg gtcggagtta
     2281 cagcttccag gcgtaggcga tgcccaccgt gtcctgatcc atgcgtgtag tgatcatgtc
     2341 tccgggtatc ggctgaccgt taggagcgag cgccggtccg gtgaccgaat tgccgaaggc
     2401 atgcacgtag gcaaacgtcc actggttacc cgatgcggtg gtgtaagtgc caccgagcgt
     2461 caggtgatcg gtgatcacgc ctggcaccag caggtcgacc atggcattgg ctggttgtac
     2521 cggattgtcg caatggttcc agccagcgcg cagcgtccag ttgtcgttgt acttgtattc
     2581 cgcacccagt ttccacacat tgatgttgtc ccagccaaag cctgggccat tgggtgcgcc
     2641 aaacgggatg ccctggaaca tcaacgccgg tgaatcgccg atggtcggcg aggtgctgta
     2701 gttgatgcgc tggtaatcga gagccagcaa ccatttcggg ttcaccttcc acgatgcgcc
     2761 cagcgcatag ttttccggca ggtcgagttc gccataattg gtgaacagcg aggcgtagcg
     2821 cgccatgcga cccatgacca ccttggtcga gtaatcagcg cccagtgtca ctgcaggtgt
     2881 gatgcgatac aggtaaccca ggcgtaagcc ggtaccggtg gtgctgtcgg tgccggcatt
     2941 ctgcacaccg aacgcctgca atccctgagc ggaaaactgc tggtagccga acagtggtgc
     3001 gataccgatc gactggttgt cggtcacctt gtaagacagt gtcggcgcaa tgatcatctg
     3061 gatcagattg acgcccagat tgccctggcc accgagcagg ttatagggcg tgcccttgat
     3121 gttgcccgga taatccgagt tcatgccgcc attgccatac acgctcaccc ccagcgcaag
     3181 ccggctgttg agcatgtggt tgtagccgaa ctcaggaatg ggaaagtaat tggcgccgct
     3241 gtcggcaccg gccggtccca gcgagccgtt gaaattgctg ctgtaagccg agcggctcgg
     3301 actgaacaga ctcatgccca ggtcgatacg attaccgacg aaggccatgc tggcggggtt
     3361 gttggcccca ccaaacgtgt cctcggtcat ggcggtggct gcgccgccca tgccgttggc
     3421 tgtcatgcca tacccggaag aaaacatgcc gtcagtggct acggcgctcg cggacagggt
     3481 cagtccggta cacagcacaa tccattgcgc ctgattgaaa cgcatttcct gctcctctag
     3541 atttagtcca tggtgttgtt ctgcactgcg gagccggtgc catcaggatc gtgacactgt
     3601 gcagccgatt ctcttcgcgg aatttgtgca ataaaaagcc gggggcggtt gcccccggcc
     3661 cgttcgcggc aatacgcccg ggttacagct tccaggtgta ttcgataccg accgtatcct
     3721 ggtacatgcg gatggtgtcg gtgccataag gcgtcggaac accaccggga taagccgtac
     3781 cggcgcccga gagtgaattc tggaacgcgt ggatgtaggc aaaggtccac tcgttgccgg
     3841 aagcggtggt atagcttccg cccagggtga tatggtcttc gatgactccc ggagcaagca
     3901 cgttcagctg gacattgctg gcggtgaccg gattgtcgca atggttccag ccggcgcgca
     3961 gcgtccagtt gtcattgtac ttgtattccg cacccagctt ccacacaccg atgtcctgcc
     4021 agccgaaacc cgggccattg gtggcgccga acggcagcgg cactgacgac gaatcgccga
     4081 tggcggcaac gccgctgtag ttgatctcct ggtaatccag cgccagagtc agatccttgg
     4141 tcgtcttcca ggcgagaccc accgagtagt tttccggcat gtccagttca ccctggttgg
     4201 caaacagacc ggaataatcc gacatcttgc tcatggtgat cttggaggcg tactgggcac
     4261 cgaaggtcag cgtcggggtg atcttgccca tgtagccgac acgcacaccg acgccggtcg
     4321 aactgtcggt tccggtgttg gtcatgtggg tcggattgga agagaaccct tcaaacccgc
     4381 tcagaccttg cgcggtaaac tgctggtatg ccaacagcgg cgcgataccg atggagttgt
     4441 tgtcgttgat tttccacgag gccgtcggcg caacgatcat ctggatcaga ttgacgccaa
     4501 gattgccttt gccaccgaaa aagttgtagc cgttggccat tggcgcgaag tcggtgttca
     4561 tgccaccgtt accgtacata ctgacgccca gcgcgacgtc cttggtcacc atgtggttgt
     4621 agccgaattc gggaatgatg aagtagttgg cgtcgctcga ttcactatac gcgctaggag
     4681 taccctgata tacgctggcg ctgcggcgcg ggctgaacag gcttgcgccc aggtcgagcc
     4741 ggttgccgac aaaagccatg ctggccgggt tgttcgcacc accgaaggcg tcttccgcca
     4801 ttgccgtggc tgctccgccc atgccgttgg cggtcatgcc gtagccgctg gaaaacatgc
     4861 cgtccgtggc atgcgccgca aacggcattg cgagacccgc aacggccatt gcggccatca
     4921 gtctgttcat tttcaccatg tcatctccgt taaggtttta attatggaat ggaatacaat
     4981 cgggttagca caagcatcac ttaatggcct gtgcgggtat ggtaccccct gtcacccagt
     5041 gcgaacaagg gggaatgcgc gccttggaca agaaaatgac tgttttgttg cacccgcaca
     5101 acgcgaacgg gccggcgtca cccggcccgc acgggaaacg cgcttactct tcgatgaaac
     5161 ctgcgccgac atcctcgcca gccatctgcc gacgcaccat atcgacgtcc tggtgcgcca
     5221 gcgcaaacgg gaacgaaaag atgtccgatg gcgtcgagtc gccgaacgga cggttgcagg
     5281 ccgagatttc ctcatcgtcc ttgccggggc aaccggaagt ctggaatggc tttcccgact
     5341 ggatgagtgt ctgcagttca tcttgcggca tgccaaagtc gatgacccgg ccctgctcat
     5401 cgaactgcat gcgattcagg tgtccgccgg cataatcgat gatgaagcgc gccagctgga
     5461 cacgacgcca ttggccgcga tcgcaggcag gccagtcttc catcatcgac ccttgttccg
     5521 gaaagaaggc aaacatgtgg ttgtgcccgc ccatgtcctt gattttctgg cacacttcaa
     5581 gcatctcgcg ttcagtttcg cccatgccgc agatcaggtg ggcgccgaac ttttccggac
     5641 cgtagatttc agccgcccat tcgatggctt gccagtattt cgcataggtg tgcgggctgt
     5701 cgaccg
//
LOCUS       DAIANL010000004        11501 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_207771, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000004 DAIANL010000000
VERSION     DAIANL010000004.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 11501)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11501)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11501
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207771"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2014 a         3376 c         3808 g         2303 t
ORIGIN      
        1 tctttcatcg gcgtggacgg gatcaatgcc acgctcaatt cgggtcgcat gcagatcgtg
       61 ctcaagccat tggcacagcg cggtgtgagc gccacggcgg tgatccggcg gttgcagccg
      121 cagctggacc gtgtggctgg catcagcatg tacatgcaac cggtgcagga cctgaccatc
      181 gaggaccaga tcagtcgtac ccagtaccag ttcaccctga ccgatgccaa tccgctggaa
      241 ctgaccgact ggaccaaccg gctcgaagcg cggctcaaga ccttgccgca gttgactgat
      301 gtggccagta atctgcagga tcacggtttg caggcatatc tggaaatcaa ccgcgatacc
      361 gcaggccggc tcggcatcac cgcagccgcc atcgatcagg cgctttatga cgcctatggt
      421 cagcggctgg tgtcgacgat ctatacccag tccaacatct accgtgtggt gctcgaagtc
      481 gagccgcgct atcgccatac gccacaaggg ctgaactcgc tgtacctgat gtcgaccgcc
      541 ggcacgccga taccgctggc ggaagtcgcg cggctggtgg tgcgcccgac atcgctggtc
      601 atcaaccaca atggccagtt ccccgccgcc acgctgtcgt tcaacctgcc caagggcgtg
      661 tcgctgggcg aggcggtcga tgcgatccgc gctaccgagc gttccatggg catgccgttg
      721 cgggtgcaga cccagttcga gggcgcagcg ctggcgtttc aggcgtcgct gtcgagtacg
      781 ctgtggctga ttctggctgc catcgtcacc atgtatatcg tgctcggcgt cctgtacgaa
      841 agttacatcc acccggtcac catcctgtcc acactgccgt cggcgggagt gggcgctttg
      901 ctggcgctga tgctgagtgg caagcagctc gacatcatag ccatcatcgg catcatcctg
      961 ctgatcggta tcgtcaagaa aaatgccatc atgatggtcg attttgcgtt gcatgcggaa
     1021 cgtgtggagg gcaaaccggc acgcgaggcg attttcgaag cctgcctgct gcgcctgcgg
     1081 cccatcctca tgaccaccat ggcggcgtta ctggcggctt tgccgctgat gctcgggggt
     1141 ggggtgggca gcgaactgcg cgctccgctg ggcatcacca tggtgggggg gctgctgatg
     1201 agccagttgc tcacgctgta taccacgccc gtgatctacc tgctgtttga ccggttggca
     1261 cggcggctcg atcagcagcg cgagcgctgg caggcggcct gagcatgggc ttcgttggct
     1321 ggttcatccg ccgttcggtc gcaaccacgc tgctggccat gggcgtggtg ctggctggtg
     1381 cactggcatt ccgcgccctg ccagtcgcgc cgttgccgca gatcgatttt ccggtgatct
     1441 cggtgcaggc gtcgctgccg ggagccagtc cggaaaccat ggcaagcacg gtggccacac
     1501 cacttgagcg ctcgctcggc gtgattgccg gtgtcaacga aatcacctcg agcagttcgc
     1561 tcggcagcac cagcatcaat ctgcagttcg acctggggcg agacatcaac ggtgccgcac
     1621 gcgacgtgat ggctgcaatc aacgctgcgc gcaccctgtt gccatccggc atgcccaaca
     1681 acccgattta ccgtaagatc aacccggcgg atgcgccgat catgatcctg gcgctgactt
     1741 cgaaaaccct gaaccggggg cagttgtacg atgcggcctc gagtatcctg gcgcaacgcc
     1801 tgtcgcaggt aaatggcgtc gggcaggtga cggtcggcgg ctcggccttg ccggcggtgc
     1861 gtgttgacct cgatccgggc cagctcaacc ggtatggcat cagtgtcgaa caggtgcgca
     1921 gtgcgatcgt ggccgacaac ccaaaccgcc ccaagggtag cctggaggac ggcgagcacc
     1981 gctggcagat tggcgccaat gaccaggtca agacggcggc tggctatcag ccgctcatcg
     2041 ttggctatga cagtgtcact ggtgcgccgg tacgcctgtc cgatgtggct gtggtgaagg
     2101 attcggtgca ggacattcgc aacatcggcc tgttcaatgg cacacctacg gtcggtatcc
     2161 tggtgttccg cgagcctggc gccaacatac tcgataccgt ggcgcgggtg cgcgcgttgc
     2221 tgccgatgct gcaggcatcc atcccgtccg cgatcaaact gtcggtggtg atggaccgca
     2281 cgccggtgat ccgggcctcg ttggcggaag tggaacgcgc gctgttgctg tcggtggtgc
     2341 tggtggtggc aacggtattg ctgttcctgc gcaatgggcg tgcggcgctg atccccgggg
     2401 tggccgtccc ggtgtcattg atgggtacgt tttccatcat gtatctggcc ggattcagcc
     2461 tcaataccat ttcgctgatg gcgttgacga ttgctaccgg gttcgtcgtc gacgacgcgg
     2521 tggtggtggt ggaaaatgta tcgcgtcaca ttgaaaacgg gttgccgccg ttccgtgccg
     2581 ccctgaccgg gacgcgcgag gtgggcttca ctgtgctgtc gatgagtctg tcgctcattg
     2641 ccgtattttt gccgatcctg ctcatgggcg gtatcgtcgg ccggctgttc cgcgagtttg
     2701 ccgtgaccct gtcggctgcg gtgctggtgt cgatggtggt ctcgctcacg ctgacaccga
     2761 tgatgtgcgc ctgggtgttg cgccctggcg gtcagccagc cagcaccttc cgtgtcgggc
     2821 ggctggtcgc ccggctgtat gcagcgatgc tggagggtta gcggcgctcg ctcgactggg
     2881 cgctgcgtca cggtgtgtgg atgttgctgc tgctgttcgc cgtcatcgtg ctcaacattt
     2941 acctgtacat tgtcgtgccg aaaggttttt tcccgcaaca ggacaccggc aagctgatgg
     3001 gtggcatcaa ggccgaccag agcatttcct ttcaggccat gcgcggcaag gtggcggaac
     3061 tggtgcgtct ggtcggacag gatcctgccg tgcagaacgt catttgtttc agcggtggcg
     3121 aacgcaacaa tgctcatatg ttcatcgtgc tcaagccgtt gtcggagcgc aggctgtcgg
     3181 cagaccaggt gatcgcccgc ctgcgccgga aactggcgca tctgcccggt atcaaactgg
     3241 tgctgcaggc ggtgcaggat ctgcgtgtgg gcgcgcgttc gagcaatgcc cagtaccaat
     3301 ataccttgca ggcggacgat atcggtcagt tgcgattgtg ggaaccgagg ttgcgcgatg
     3361 ccatgagcca tatcccgcag attaccgata tcgataccga ccagcaggac aaggggtcgg
     3421 aggtgtggct gaacatcaac cgcgatgcga tggtgcagta tggtctgacg cagcgcgtgg
     3481 tggacaatac gctcaatgac ctgttcggtc agcggccggt gtcggtgatc taccagccgc
     3541 tcaatcagta ctgggtggtg atggaagccg cgccgcagtt ctggcagcat ccggagaacc
     3601 tgaatcaggt gtatgtgctg tcctcgacca acgtgccggt gcccctgtcg cagttcgcca
     3661 ccttcatccg cagtacggtg ccgctcgaag tcaatcacca ggggcagttc attgccacga
     3721 ccatttcgtt caaccttgcg cctggcgtgt cgctgtccac ggcgctgcag cgcatccatg
     3781 atgccgcatt gcgcatcggc atgccggaca cggtgcgcgg tggattccag gggactgcca
     3841 aggcatttca gcagtcgctc ggcaaccagc cgctgctgat tctggctgcg ctgcttgcgg
     3901 tgtacatcgt gctgggtgtg ctctatgaaa gcgtggtgca tccacttacc atcctgtcca
     3961 cgttgccatc ggccggtgtg ggtgcgctgt tggcattgct gctgttccat ttcgaattca
     4021 gcatcattgc cctgatcgga gtgatcctgc tgatcggcat cgtcaaaaag aatgccatca
     4081 tgatggtcga ttttgccatt tccaccgaac gcgaacaggg attgacgtcg cgcgaggcga
     4141 tttatcaggc ctgtctgctg cgttttcgtc cgatcatgat gaccacggtt gcggcttcgc
     4201 ttgcggcgct gccgctggca ctcggtcatg gcgacggggc ggaactgcgc cagccgctcg
     4261 gcatttcgat cgtcggcgga ctggtgctga gccagttgct tacgttgtac accaccccgg
     4321 tgatctacct gtatctggat ggcatgcgcc tgcgactgcg ggcgtggtgg caacgccgta
     4381 ctgtgatggc gtcggcgagc ggggcatgac cggctggtga atacattgca aggacagtca
     4441 gatgcgggac gtacagagcg cgcggcacgt ggcgacaggg attcgtcgag gcgaggaacc
     4501 acgcggcact gtgccgcaca tggtctggta cggcgccgct gggcgggtaa cggggaaatg
     4561 cgcatgaaaa aaacaggtgt tgctctgttg ggcggctggt gtgtcatggc gctggctgga
     4621 tgtgcgatgg ggccggacta tcaccggccg tcggtggata cgcctcccgc ctggcgtgaa
     4681 atggccggct ggaagcaggc ccagccgcag gcggtggtga gtggtgactg gtggaagatc
     4741 taccatgatc cggtgctcga cggactggag cgccaggtca gtctgggcaa tcagacggta
     4801 cagcaggcac aggcgcagta ccgccaggcg gccgctctgc tctccgccac catggccggt
     4861 ttctggccga cgatcacggc aggcacttcg tcgacgtcga gccagaccaa ttcgaccatg
     4921 tataccgtga ggaattcacc cacggtgact ctggacaagc tgtcgctcaa tgccaactgg
     4981 acgctcgata tctggggcga actgcgtcgc gaggccgagg cgggcgaagc caccgccgca
     5041 gccagtcagg cgacggctca ggcggcgctg cttacagctc aggcgacact ggccgaatcc
     5101 tacctgcaat tgcgcgtgct cgatgccgat gcgcgtatgt tgcgcaacac ggtcgaggcg
     5161 aaccagcaca cgctcgatat tacgcgcaac ctgtatgcgt caggcgtgga taccgaatac
     5221 gatgtggcgc aggcacaaac ccaggtcaag accgtacagg cgcagttgat cgatcttggg
     5281 gtgctgcgcg cgcagctcga acatgcgatt gcgctgctga tgggcaagcc ccctgccgaa
     5341 ttccgtcttg cggagagcga tgcggtgccc gcgctgccgg acattcctgc cggcgtgcct
     5401 tcccagttgc ttgagcatcg cccggacatt gccgccgcag aacggcagat ggctgcggcc
     5461 aatgcccaga tcggcgtagc tcaggcggca ttctttccca cgctgacgct gggcggggtg
     5521 ttcggttacc agagcctgag ctacagcaat ctggtctcga cgcccaaccg ctactggtcg
     5581 tatggtccgg cgttggtggg taccctgttt gacggcggta tcctgagcgc gcagaaggcc
     5641 caggccgtgg cggcttatga tgcaagcgtg gccaattacc gccagacggt gctgaccggt
     5701 tttcaggagg tggaggacga tctggccacg ctgcacgtgc tggcccagga aaacgacgtg
     5761 cagcgccagg cggtcgagtc ggcgcggcgg gcgctggcag tgtcgcagca tcagtatgag
     5821 gcgggtacag tgaactatat caatgtgctg gcggcgcaga ttgtggcgct gtcggcggag
     5881 aaagcgcaga tcgacattct aggccgtcag ctggtggcga gcgccggact ggtgaccgcg
     5941 ctaggtggct tgtggtaaac gcgctgctgc atcgtcgagc aactgccgaa acgaggtgaa
     6001 cagccgtttc atgaccgggg cgcggtaagg aaacatctcg ggcgcatcga tggcatgcac
     6061 aaatgcgcgg ttgtcgagct cgaataccag cagacggcca tcggccgttt cggcgcagtc
     6121 catcaccaca taatcgagac cggtgcgacg ggcgattgcc tgcaatgcgt cctggtggcg
     6181 cacaccgaag ccggaatcga atgtctgcat gaacacggct tcctcctcgc gcttggacgg
     6241 ggtggtgtcc atgcctgcat ccttgtaatg caccatccac tccgcggcga ttgccatgtg
     6301 gcaggcgtaa gcgcgtccgt tgcacagtgc aatgcggtat ttgcggtgct ggccgtcggc
     6361 gctgcgataa tcgacgaaac tggcgaggta aaaatgcgtt gcagcctgtc cggccagata
     6421 gtcggccagt tgctgcggcg attcgatttt cgccaggccg cgacccccat gggcgtcgag
     6481 cggacggatg atgcagggga aggccgtacc gggcaggggc gcatggcctg tggccggagc
     6541 cgacaacgca tcgcgtgaca acaggtgcgt ggcagggaca tacagggtgg gaatggcgcc
     6601 cagcagcgtg tgcagctggt gccgggccag ttgcacgatg ctccgcggat cgttgatgac
     6661 cggccgtggc caaccggaca gctgcgtagc cagctgctcc agccagggct tgcttgccgt
     6721 cgattcgccg atggcgacaa acaatacgtc gtgtggtggc agttgtcgcg gcaggggttg
     6781 gtgcggttgc aggtacagcc agtacatgtc cacgtcgctg tgttccagca ggaagtcgac
     6841 cggcgtgttg tccatcatgt cccccggctg catcagcacc agcagccgca gccgcgccgg
     6901 ctgctggctg gcggccacat ggtacagtgc gcagcggtgc agggcgcgct gctgcatgtc
     6961 cagtccgcct tcgcggttac cggtcagctg gaggatctgc gccagattca tcagcaggcc
     7021 ggcatcgtcc gggtgttgca tcgcgcgggt caccagttcc gcaagcggaa agccggcctg
     7081 cacccggcga aacaggccgg cgaggctcat cagcggttgc gggtcgaacc cggtggcggt
     7141 cgcgttcatc gggtcgggga aaaaatcctg gggctcatac cacgcagcat agcggttgcc
     7201 cgtgcgccgg gcaaccgctg tcacagtgtc gcatggcggc acgggccgga ttgacaaagt
     7261 agttcacccc gtaataatct atgcatgaat caatttccgc cctgtgtcgg atatgtacaa
     7321 gcagacgata acaagacagt acaggatgaa tggtatgcgg ttgtgctgga cacacatggt
     7381 gctggccgtt gcctgcgcgc tggccctggc gggatgcgcc agcgatgccg ggctggcacc
     7441 gaccgcgcaa ccgaccgggc tgccgcgcca gcatgccggt gaagtcgata gcctgtggtg
     7501 gaagcgtttt ggcgacgctc aactcgacgc gctggtggac gatgcgttgc aacatgcccc
     7561 cgatatcgcc ctggccggtg cgcgtattcg cctggcgcaa cgccagggcg aactggccgt
     7621 ggcccagctg aacccctcgt atgcgatcga tgcctccgcc cagcgcgagg agttcagcgc
     7681 gctcggttat tttcctccac ccatcggcgg cagtgtgttc aatctgggaa cggtgcaggc
     7741 tgatttcacc tgggatctcg actggtgggg caagcatcgg gcgacgtggc ggctggttgc
     7801 cggtgagacc aatgctgccc gtgccgaaga acgcgaagcc aggctggcgc tcagtatcag
     7861 catcgcgcgc agctatttcg ccctgcaggc cgatagtgcc cggctggcga cggtgaacgg
     7921 catgctgcag gaccggcagc aagtactggc tatccagcag gaacgccagc gtgccggact
     7981 cgaatcggat cgcccggtca ggccggcgca gggcagcatc gcggcgctcg aacgccaggc
     8041 gcaacaactg cgcgcgttga tccaggccga ccgtatcgcc attgccgctc tggcaggaca
     8101 gccgcccgcg cgcggtgatg ccatccgcgt ggctccgctg cctgccgtac cggagttgcc
     8161 ggcgtcgctg ccggcagacc gtatcggatt gcggcctgac gtcgcaatgc aacgggcagt
     8221 gatcgaagtg gccagcgcgc aggcgcggct tgccagaatc cagttctacc cggacatcaa
     8281 tctgtcgggg tttgccggtc tgcagagcat tggtttcgaa aacctgctca agtcgggcag
     8341 cgaaaccttt gccgtcggac cagcgtttca cctgccgatc ttcgaccagc acagcttgcg
     8401 cgccaatctg ggcgcacgct acgccgaagt cgatatcgcc gtggagacgt ataaccggac
     8461 ggtgatcgag gccatgcgcc aggcggcgac ggcgaccatt cgcctgcagt ctctcgggcg
     8521 cgaggcgcaa tcgcagcaac ggctggttga cacgctggac ggatcgcgca gccttgccga
     8581 agcgcgctac cgtcaggggc tggaaaacga tttgcctgtc ttcgaggcga atattgcctg
     8641 ccagaatgcg cgcaatgctt cgatcgaact gcaacagcag cggctggagg ccgtgctgga
     8701 cgtggtgcgc gccatgggtg gctggccgaa tcaaaaatcg ggtgaaacac gatgagcatg
     8761 cagaaaaaac gcaagacaat gttgctcctg ctggggttgg tgtttgtact ggcggcagcc
     8821 tgggcactgt ggcactggct ggaatatggc cgttatgaac aggatacgga tgacgcctac
     8881 gttgcaggca acctggtaga ggtgatgccg caactgtccg ggaacgtgca ggcggtctat
     8941 gccgatgaaa cgcaacgggt ggacgtcggg cagccgctgg tgcagctcga tggtgcggat
     9001 gcggctctgg cgttggataa agcggaggcc acactggcgc agactgtacg caacgtgcgt
     9061 caaatgatgc aggatgcccg cgcggcggaa gccttggtgc agcagcggca ggccgaatgc
     9121 gacaaggcac gcagtgacct gcagcgtcgg gcaagcgtgc tcgatgtgcg tgccgtggcc
     9181 agggaggatg tgcagcatgc gcgtgatgcc ctgacggctg caacggcagc gctggtagcc
     9241 gcaaaatatc agcgggatgc ggcgcaggcg gctgtggccg gtacagtgct cgaacgccat
     9301 ccggcggtgc tgcgcgccga agcccagttg cgggaggcct ggcttgcgca gcaacgcctg
     9361 accatccgcg caccggtgag cggttacgtg gccagccggc cggtgcaggt tggcgagcgc
     9421 gttgcgcctt cgaccgtgtt gatggtggtg gtgccgttgc accaggtctg gctcaatgcc
     9481 aacttcaagg aaaatcagct caaacgcgtg cgtatcggcc agccagtaca tatcgtcacc
     9541 gatctgtatg gtagcgatgt ggtgtttcac ggcacagtgg ccggattgag tgccggtacc
     9601 ggcacggtgt tttcgttgtt gccggcacag aacgcaaccg gaaactggat caaggtggtg
     9661 caacgtttgc cggtgcgcat cgaaatcaat cagaccgagc tggggcggta tccgttgcgc
     9721 atcggcttgt cggccacggc cagcatcgat gtgcatgatg ccagtgggcg catgctggcc
     9781 acagggcagc cggtcgcgcc gctgtaccag accccggtgc tggccgagga tctgtcgggc
     9841 gccgatcgac tcatcgcacg catcgtcaag ttgaacgggc acccatgagt cagcatccgc
     9901 cgatacacgg tgcggcgttg gccctgctga cgctgtcact gtcgctggcg accttcatgc
     9961 aggtgctcga tacctcgatc gccaacgtgt cgattccgga catttccggc gatctggcgg
    10021 tgagccctga tcaggggacg tggatcatca cttcgtttgc ggtcagcaat gccattgccc
    10081 tgccgttgtc gggctggctg gcgcaacggt tcggtgaggt gcggctgttc gttgcggcca
    10141 cgctgctgtt cacactggca tcgatgttct gcggcctgtc caccaacctg cccatgctgg
    10201 tgacgttccg cgtcatccag ggggcggtgg ccggtccgat gattccgctg tcacaaagtc
    10261 tgctgctggc caattacccg cacgagaaga aggggctggc gctggcgtta tggtcgatga
    10321 ccgtggtgct ggcgccgata tttggcccga tcctcggggg ttacattacc gataatctca
    10381 gctggccgtg gattttctac atcaacgtgc cggtaggtat cgtgtcatcc tggttcacct
    10441 ggcagctgtt gcgcgagcgc gagacggaaa cccatcgtcg gccgatcgac cgcgtcggtc
    10501 tggtgttgct ggtgatcggg gtcgggtgcc tgcaactgct gctcgacaag ggcaatgaac
    10561 tcgactggtt cgactcgcgt accatcatcg tgctggctgt ggtggccaca atctgcctgt
    10621 cggtattcgt tgtctgggaa ctgaccgaaa cacatccggt ggtcgatctg agcctgtttg
    10681 ccatacgcaa ttttactgtg ggcgccatcg ccatcagcat cggctacatg acgttctttg
    10741 ccggggtggt ggtctatccg ctctggttgc agacgcagat gggctacacg gcgacctggg
    10801 ccgggctgtc ggcagcgccg ctcggcatcc tgccggtgtt tctgtcaccg ttcgtcggcc
    10861 gttacatgca tcgtatcgac ctgcgtgtgc tggtgagttt cagttttgtg gtgttcggtg
    10921 cagtatcgct gtggtcgtcc accttcgata ccaatgccga tttcctgcac ctgaccctgc
    10981 cgcgcttcac ccagggactg ggcatggcgt tctttttcat tccgctgatg tcgattctgc
    11041 tgtctggctt gccggcaaac cgcgtggcga gcgcgtcggg attgtcgaac ttcctgcgca
    11101 tgctgggtgg cagttttggc acctcgctca gcattgccct gtgggaccgt cgcgagtcgt
    11161 tccaccacag ccggctcacc gaaagcctga ccgctaccga tcccgcagca caatccgcag
    11221 tagctggcct gcatgccgcc ggcatgccga cggcctcggc atgggcagtg attgaacgtc
    11281 agctgagcgg ccagtcctat gtgctggcgg taaacgatgt ctttctggtg tcaggctgga
    11341 tattcttttc gctgattgcg ctgatctggc tggcgcggcc gccattcact tcagcgaggc
    11401 cgggcagcgc ggccgcagaa cattagagca ggccgttgaa aaaggtagcg aggacgcccg
    11461 gatgcaaggc gcgagaaacg caacgacgag acatatcaaa t
//
LOCUS       DAIANL010000005        34093 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_617582, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000005 DAIANL010000000
VERSION     DAIANL010000005.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 34093)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34093)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..34093
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617582"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         6514 a         9596 c        11134 g         6849 t
ORIGIN      
        1 gatcagtcga tacatgccgt gtgccggggt tcaggccaga gcggtctgca gacgctgaat
       61 ggcggattcg agctgggaca tcgatgtggc gaacgacagc cgcacatagc cctcgctacc
      121 gaatgcggaa cctggtacga cggccacgcc ggcctgttcg agcaggaatt cgctgaatgc
      181 gatgtcagtg gcttcggcaa tgcggccttc atggtgcagg cgatggatgg cgcagctggc
      241 atcggggaat gcgtagaagg cgccgcccgc atacaggcag tcgatgccat gtatttcatt
      301 gagcccgcca accacgaatt cgtggcggtt gcggaaggct tccaccatgg gtcggatgca
      361 gtccgggtcg ccgttgagcg cggcctcggc agccacctgc gagatcgagg tcgggttgga
      421 ggtggactgc gactgcacgt tttccatcgc agtgataagt tcgaccgggc cggcgcaata
      481 accgatgcgc cagccggtca tggcataggc tttcgacacg ccgttgagca ccacggtgcg
      541 cggatagagg tcagggcagg cgttgagaat gttgcagaac ggctggtcgt gcagctggat
      601 atgctcgtac atgtcgtcgg tagcgatcag cacgtgcgga tagcggcgca gcacttcgcc
      661 cagagcggcc agttcttcca gagtatagac ggcgcccgaa gggttgctcg ggctgttgag
      721 gactacgagc cgcgtgcgcg gtgaaatggc cgcgtcgagt tgttgcggac tgatcttgaa
      781 tccctgcgcg atgccggtgt gaagaaacac cggcgcgccg ccggccagca ggacgatgtc
      841 ggggtaggag acccagtagg gcgtggggat gatgacctcg tcgccgggat tgatcagtgc
      901 ctgtaccagg ttgaagaagc tttgtttgcc gccgcacgaa accagaattt gcgctgtggc
      961 gtattcaaaa ccgttttcac ggcggaattt gtcgacgacg gcctgcttga gcgagggcgt
     1021 gccaccgacg gcagtgtatt tggtgaagcc gcgccggatg gcgtcgatgg ccgcattctt
     1081 gatgtgttgc ggggtgtcga aatccggttc gccggcaccg aggctgatga tgtcgcgtcc
     1141 ggcagctttc aggcgcgcag cgcgcgcggt gacggccagg gttggcgaag gtttgatagc
     1201 ctgtacgcgg tgagagagtt ccactttgaa ttcgtcctcg aagtcggccg cccggcggaa
     1261 aagcgcgcga cggcgtgata gtatggcaag ttcatgcggt cacgtaccgc aagatggttt
     1321 tcatggtttt tgaacagggg gattttaccc gtgatcgcac ttttccccaa tagtcccttc
     1381 cgcctgcatc agccatttcc gcccgccggc gatcagccgc aggccatcga acatctcgtg
     1441 gatggagtgg aagatggact ggcctggcaa accctgctgg gcgtgaccgg ttccggcaag
     1501 acctatacca tggccaatgt catcgcgcgg ctgggccggc cggccatgat catggcaccc
     1561 aacaagacac ttgcagccca gctgtatgcc gaaatgcgcg agttcttccc ggagaattcg
     1621 gtcgagtatt tcgtgtcgta ttacgattat taccagcccg aagcttatgt tccgtcacgc
     1681 gatctgttca tcgagaagga ttccagcgtc aacgagcaca tcgaacagat gcgtctgtcg
     1741 gccaccaagg cgctgctcga acgcaaggac agcgtgatcg tagccactgt ttcggcgatt
     1801 tacggcatcg gcgatccggt ggactatcat cagatgatcc tgcacctgcg ccagggtgag
     1861 cggctcaacc agcgcgatgc gctgcagcgg ctggtggcga tgcagtatac gcgcaatgaa
     1921 atggaattcc gccggggcgt cttccgcgtg cgtggcgacg tgattgacat ctttccggcg
     1981 gagaatgccg agcacgccgt gcggctgtcg ctgttcgacg acgagatcga agcgattgcg
     2041 ctgttcgatc cgcttaccgg cgccattgag cgcaaggtac cgcgcttcac tgtctatccg
     2101 tccagtcatt acgtcacacc gcgcagtacc acgttgcgcg cgatcgaggc catcaagctc
     2161 gaattgcgcg atcgactcga tgagctgtat cgcgacaatc gcctggtgga ggcacaacgg
     2221 cttgaacagc gcacgcgctt cgatctggag atgatgaacg aactcggctt ctgcaagggc
     2281 atcgagaatt attcgcgcca cctgtccggt cgcaaggccg gcgaaccacc gcccacgctg
     2341 atcgactacc tgccggtcga tgcgctgatg atcatcgatg aatcgcatgt caccatcccg
     2401 cagatcggcg gcatgtaccg cggcgatcgc gcgcgcaagg aaaatctggt gcactacggg
     2461 ttccgtctgc cttctgcggt cgataaccgg ccgctgcgtt tcgacgagtt cgaacgcatc
     2521 atgcgccaga cgattttcgt ttccgccacg cccgcagaat atgagcgcac gcaccaggcc
     2581 caggtggtgg aacaggtggt gcggccgacc ggcctgatcg atccggagat cgaagtgcgc
     2641 ccggcctcga cccaggtgga tgacgtgttg tcggaaatcc gtcgctgtac ggcgctcggc
     2701 gagcgggtgc tggtgaccac gctcaccaag cgcatgtcgg aaaacctgac ggagtttctt
     2761 ggcgaacacg gggtgcgcgt acgttacctg cattccgaca tcgataccgt ggaacgggtg
     2821 gaaattatcc gtgacttgcg gctaggcatt tttgacgtgc tggtgggcat caacctgttg
     2881 cgcgaggggc tggacatccc ggaggtgtcg ttggtggcga ttctcgatgc tgacaaggaa
     2941 ggtttcctgc gctccgaacg ttcgctgatc cagaccatcg gccgggcggc gcgccacctg
     3001 aacgggcgcg ctatcctcta tggcgatcgc gtgaccgcgt cgatgcagcg ggccatgggc
     3061 gagaatgaac gccgccggga gaaacagctt gaattcaatc gtcagcacgg gatcgtgccg
     3121 cgtggcgtgg tcaagcagat caaggatatc atcgaaggcg cgcgcgaagg tgtgaccacg
     3181 acgcatggag tgcatcgtgt ggctgtggcc gagacggagg ccgggtatcg cgcccgcgac
     3241 gagaaggcgc tggcacgcga aatccgtcag ctggaaaaat cgatgcttga agccgcacgc
     3301 aatctggaat tcgagcaggc agcacggttg cgtgacgaat tgcgccgcat gaaggtggaa
     3361 ctgttcggcg tggagggcca ggatggcgcc gagttatttc ccgacgggcg cgacagcggc
     3421 cgggcagcat agcatgccag gcgtgattcg cggtcaggga ctgttcccgt cgacaagacg
     3481 tacgacacgg ccatcggttt gcctggcccc atcccagccg ggttgcattt tcttcggtgc
     3541 tccattgccc tgtcgggttg ggtttcgtgg cgctgccgac cactggtgcc ggctgtgccg
     3601 tgatgcaacc cctgtcgaca gcctcgcgat tcgtgagggt ctggatcagc gcttttccgg
     3661 gaggcgcatg agcggatacc ggcatacgca tgcgccaggc ccgcccgtac ttgccccgga
     3721 cgccgcgtcg ggttgaacgg cgatgcgtgc ggcggtgctg gcctgtgttg cgggggtgtg
     3781 ggggttgcaa cagctggctg cgctgccatc catacgttgg ctgtggttgt tgccagtcgg
     3841 tgcggtcagc atctggatgt tgccgtccag cgcgggctgg caggcggcac ggcggctggc
     3901 atggctgttg ctgtgtgtgg cgctgggatt cggctgggcc ctgtggcgtg ccgacctgcg
     3961 tctggctgat gcgttgtcgc cgcaatggag cggcatggac gtgcagctgg aaggcgtggt
     4021 ggccggattg ccgcaggcgg gagcgcacgg cgagcgattc ggtttcgatg tgctgcaggt
     4081 gctgactccg ggtgcggtcg tgccgcaaca tgtgcggctg gtgctgtccg ggcagaacgg
     4141 aaactcgtcg gtgcggcctg gccagcgttg gcgctggacg gtccggctgt ggtcgccgca
     4201 tgccagcgag aacccccatg tcgcagatgc cgaagccggc tggtttgcca gcgacgtgcg
     4261 cgccttcggt gtggtgcttc agcgtcctgt cgcggtgctg gtcgatgcgc gcgcgcaccg
     4321 tctgggcgac tggatcgacc gcttgcgtgc agccatcgac gcccgtttcg atcgtgtgcc
     4381 cggtactgcg ccctatgctg gcgtgctcaa ggcgctggcg gtgggcgatc agtcgacaat
     4441 cagcacggcg caatggcagt tgtttcagca caccgggatt gtgcacctgg tttcgatttc
     4501 cgggtcacac gtgaccctgc tggccggaat gatctatgcc ggcgtgctgt ggctgtggcg
     4561 cgcgagcatg ggcctgtcga gccgacttgc ggcgcagcgt gcagcggcac tgggcggtgc
     4621 gctggccgcg cttggctatg tagcgctggc cggatggggc attcccgcgc agcgtacgct
     4681 ggtcatgttg tgggtggcgg cgctggctca atggagcgga cgcgggctgg cacccacgcg
     4741 catgcttgct ctggcactgc tggccgtgtg cgtgttcgat ccgtgggcgg tgcttgcgcc
     4801 cggattctgg ctctcgttcg gcgcggtcgg cttgttgctg tttgtcagtg cggcgcgggt
     4861 cggtaccgtg ccgccctggc ggcaatggtg gcgcgcgcaa tgggcggtga cgctgggact
     4921 ggcgccgttg ttgctcggct ggttcgatca ggtgtcactg gtgtcgccgc tggccaatgc
     4981 cgtggccatt ccgctggtgg aaatgctggt gacgccgttg acgctggcgg gttgcgtgcc
     5041 ggggtttgat gtgccgttgc actgggccgc gacgttgtgc ggcgtgctgg tatggtggct
     5101 gcgcctgctc gaccgctggc ccgtgtggca gcctgcgata cccgcaaccg gggtgctggt
     5161 gttgttgttg gcgggtgtgt tctggagtct gttgccgcgc ggcatgccgg cgcgctggct
     5221 gggcggcctt gtctggctgc ccgtgctgtt tgcgccggct gcccgaccgg ataacggcgc
     5281 cttgtgggtg gatgtgctcg atgtggggca ggggttggcg gtggcgctgc gtacgcgcca
     5341 tcatgcctgg ctgtttgaca ccgggccgcc tggtgcaggg ggtgggtccg atagcggcga
     5401 acgcattgta ctgccctggc tgcgcggcga gggcgtcggt cggctcgatg cgctggtgct
     5461 gtcacacgac gacaatgacc atacgggggg tgcacgttcg gtgctgtcgg tgctgccggc
     5521 tggcgaggtg ttgacctcgc tgccgcgtgg tcatggggtg ctcagtacgt ttcgtggcac
     5581 ggtcgtgccc tgcgtgcagg gccagacatg ggtggcggac ggggtgcgtt ttgccgtgct
     5641 gcatccgtcg cagcgcagtt atgccgacag ccgtctgcgc gataaccacc gcagttgcgt
     5701 gctgcgcgcc gaaagcgccg gtggcagcgt gctggtggca gcggatatcg aagctgccga
     5761 cgagtcgcag atggtggcgg cttatggcgc agaactgcac agcatgctgc tggtggcgcc
     5821 gcatcatggc agccggacat catcgacgac agattttatt gccgctgtgc ggcccgcatg
     5881 ggtaatattt accgtggggg cgcataaccg cttcggtcat ccgcatggcg aagtggtggc
     5941 gcgctatcgg gcacagggct gtgcgttgtg gcgcagcgac cgtgccggac tggttgccgt
     6001 gcgcttttca ccgcaccggc cgccgcagat ttcagcctgg cgcgtcgagc accagcgcta
     6061 ctggatggat ggagtgaacg aacgatcatg gtaaccaggc aggacggcct gtcggcgccg
     6121 gaactggctg actggaaagc atcgctggca caactggcgg cggagttcga tgccgatggg
     6181 ctggcacgcc ttgacgcggt cgcgcaatgg cgcctcgacg gtcaggcatc ggccattgcc
     6241 tggcccgatg cgctcgggat cgcgatgctg gtcgccgggt tgcatctcga tgccgactgt
     6301 gtggcggccg tgctgctgct tggttgcgat acccgggatg cgccactgcc ggccgaggcg
     6361 gtcgtgctgg cgcagggtgt cgaacggctg caggatattg gcgggttgct ggccggagaa
     6421 cgccgtgacc ggcgagatgc acagggtcag gcggagaacc tgcgtcagat gctgctggcc
     6481 atggttagcg acatccgggt ggtgctcatc aagcttgccg aacggctgca tgcgctgcgg
     6541 cgcgccatcc agagcaacga tgctgcgctg cagcgccaac tggcgctcga aatgcaggga
     6601 ttgttcgccc cgcttgccaa ccggctcggt gtatggcaga tcaaatggga aatggaagac
     6661 ctggcgtttc gtattctcga tgccgccaac taccagcgca ttgcgcgcct gctcgacgaa
     6721 aaacggctgg accgtgagcg ttacattact gcagtggtcg aacggttgcg cgccgagctt
     6781 gatgcagccg gaatccgtgc gcaggtgacc ggccgaccca aacacatcgt cagcatcatc
     6841 aacaagatga accgcaagca tctggatttc gagcagatct atgatatccg ggcagtgcgc
     6901 attctggtcg atgaggtgcg tgactgctac gccgcgctgg gtctggttca ccacctgtgg
     6961 cagccgatcg ccggtgaatt cgacgattac atcgcccatc cgaaaagcaa tgattaccgc
     7021 tcgctgcaca ccggggtggt cgggccggaa agcaaggcac tcgaagtgca gatccgcacc
     7081 cacgaaatgc atcaccatgc cgaacttggc gtggcggcgc actggcgtta caaggaaggc
     7141 ggcaaatccg acgcactggt cgaacggatc gcctggctgc gcacgatcct gagctggaag
     7201 gatgaagtgg ccggtcagaa cgaactggcg gggctgctgc gcaatgaact gttccaggat
     7261 cgcatctacg tgctgacacc gcagggtcgg gtcattgatc tggaggccgg cgcaagcccg
     7321 gttgatttcg cttaccacgt gcataccgat ctcggccacc gttgccgtgg ggccaaggtt
     7381 gatggtgcga tcgtgccgct caatactccg ctcagaaatg gtcagcgcgt ggagatcctg
     7441 gcagtcaggc agggcggacc gagtcgcgac tggctgaatc cggcgctggg atatctggcc
     7501 agtccgcggg cgcgcgccaa ggtacgtcat tggtttcgtg ccgagtattt tgccgagcat
     7561 gttgctgccg gacgtgacgt gctcgaacgc gagcaacgcc gcacccgtct gacggatgtc
     7621 agtcacgaga aactggctgc ccggttaggt catgcacggg tggaggacat gctggctgcg
     7681 ctgggccgtg gggagatcgg cccgcaagtg ctggcagacg ccatgcgtgc actgagtcag
     7741 ccgatctcgc cgctgcgcga tacgacgaaa aaccggccac agccgcctgc ggtgacgcaa
     7801 tcggttctga tcgaaggcgt agccgggctg ccagtggtac tggcgcgctg ttgcaatccg
     7861 gcaccgccag cgggcatcgt ggcctatgcc accctcggcc gcggcatgac cgtgcaccga
     7921 cgcacctgtg cgaacatgct gcgggtggat gccgggcgcc aggaccggtt gctggcagcg
     7981 tcctggagcg gcgagacgca actggctcct gcggagatcc tggtggaggc gtttgatcgg
     8041 caagggttgt tgcgcgacgt gtctgcgttg cttacgcggg agaaagtgtt cgtggtgcgc
     8101 gtcaataccg aatcaagcgg cgaccttgcg gaaatgcact ttcaggtgca cgcgcatcgg
     8161 tcgcttgagc gggtgctgga aaagattcgc aatcttcccg acgtattgca ggctgtgcgg
     8221 ctgagctgaa ggtgtgtctc gacaaatctg cggcaccggc cagggctgca ttgctccgtg
     8281 ctcgtccgtt cgcctgtctg gtaccgtgct cttaagtatt tatatcaatt ttcgtcaatt
     8341 tgcagatatt ttattttgca aactccagaa aaaaatttta tttattttta cttaagttat
     8401 ttttaagcaa tattgttgct tattaagcaa tccccctaaa gttttcttca ggcggttcgt
     8461 aaatggttca gatgtcggtg attggggttg aaagttgcga tgaaaaaaga tgtgattttt
     8521 tttcaggcaa ccctaaagtt tcagaaactc catccgataa ctgctgaaac ggggcaattt
     8581 ttttaattgc tgcgtgtgcc gtaaatccgg ttttttggtt tcgccgagtc ggtgtctgtc
     8641 tgacatgaga tgcgtccggg gataaccgtt atcaacagca accggatgtt tttgttgttt
     8701 tttatcagat tgtggttctt gaggccaata aaaaattggc tgtttctgct cgtctgtagg
     8761 tttttatctg tcacgcctcc ctccctggtt tcgtacataa aatacggaat cgtgcttatt
     8821 ttcgcttttt ttcatccgta ccaatatgca gccatgccgg cggaattggt gcgcggtgca
     8881 aaccggaatc gctcaacagg atttgcatcc gcgtactgac cgcataagac ttgtgcagga
     8941 tgatttaagg gggaacgtga tgactgccaa tgacctgttg tccgagattc gcgacgccaa
     9001 tctgaattac ctgatgctgg cccagcagat gatccgtgcc gatcgtgaga cggcgatttt
     9061 tcgcctgggc gtcgacggtc agattgccga gctgctcgaa gggttgagta atgctcagct
     9121 gttgcgactg gcatcgagca acatgatgtt ggcgcggttc cgtttcgagg acagcgccat
     9181 cctgggcatg ctgacgcatc cgcacaagga tcgtgctttg gggcatgcgc attctgccat
     9241 cctcatggcc ggtcagccgg tcgaagaagc gcactgatac ggaggccgcc atggcaaaaa
     9301 ccagcgtcat gaccgaagcc caggatattc agcttgccat cgacctgatc aggctgggcg
     9361 cacgcctcca gttgctggag tcggagacat cgttgtcgcg cgagcgtctg ctcaagctgt
     9421 acaaggaact caagggtgca tcgcctccca aaggtatgct gccgttttct tcggactggt
     9481 ttctgacatg gcagccgaac attcatgcgt cgattttcat gaacgtctat cgctacctgc
     9541 agcgtcacgc tgatgtggac ggtatccagg cgatcatgaa agccttccgt ctgtatcttg
     9601 agcaggtcga cccggtggct gagggtgaag aaccggtgtt gtcgctcacc cgggcctgga
     9661 cgctggtgcg tttcatcgac cacggcatgc tgtcgatggc gcgttgtgac aagtgcggcg
     9721 gccatttcgt ggttgaccgc ttcgatctcc acgcgcatta tgtgtgcggt ttgtgtcaca
     9781 tgccatcgcg cgcgggaaaa acccgcaagg cgcgcgaaca gacggcgctg caggcggcgt
     9841 gctgagtcgc gcatcgcccc ggtggctgaa ttccggggtg gaagcaggtt gtcagattct
     9901 ctcaggtttc gtttcatccg ctggcccatg acacaggcgc tggccatcca ggccggcgtc
     9961 gtgctgtttc aggcgctggc ctggtggcta tggttgcact ggtggcccag cgcatcgcct
    10021 gcattggggg tggttgcgct ggtgcagggc gtgctctcgg ccatgatttc tttctggatc
    10081 ggcatggcgc tgtggtggtg gctgatccag ctgactttcc cgctggtcgt gctggctgcc
    10141 gtggccgtgc attggccggt cgcagtgtac gccggcctgt tcagcgtgct gtggctggga
    10201 tactggcaca cattccgcac gcaagtaccc tattacccct cctcaccggc agtatggcgc
    10261 gcggtgcagg actgcctgcc ggccgggcgg ccatgtcgtg cgatcgatct cggcagtggc
    10321 ctgggcgggc tggtgctgca cctggcgcgc acgcgctccg actgtcgcgt cacgggagct
    10381 gagctggcac cactgccgtg gctgacgagt cggttgcgcg caatggggct gccaaacgta
    10441 ttcctgcggc gcgtggatta ccattcactg gatctgggcg agtatgacct ggtgtttgcc
    10501 tacctgtctc ctgcggcgat gcctgcgctc tggcgcaagg cgcagcagga aatgcggccg
    10561 ggcagcgtgc tgctcagtta tgagtttgtc atcccggatc agccggcaga tcgtcagatt
    10621 cacctgccag gcatgcattc cttgctgtat gcatggtata tttgacgcgt tgtaggggag
    10681 attgctgtca tgcaggccgg tacggcaatg cctgcagaaa tgaccggcgt tgaacgggga
    10741 gtgctgagtg tttgtcatta tcggttatgc agtggtgctg agcgcgatat tcgggggatt
    10801 tgcgctcgct ggcgggcatc ttgcggcatt gttgcagccg atcgaactgc tgatgatttt
    10861 tggcggcgcg atcggagcgt tttttgttgg aaacaacaac aaggctgtca aggctacggt
    10921 caaagccctg cctacggttt tcaaggggtc ggcctacacc aaggcgttgt atatggattt
    10981 gctggccctg ctctacgatc tgttgaccaa ggtgcgcaag gaagggttga tgtcgatcga
    11041 aaacgacgtg gaaaatccgg agagcagccc gttgttcggc aagtacccga atgtgatcaa
    11101 ggatcaccac gtggtcgaat tcatgaccga ctacctgcgc ctcatggtgt ccggcaacat
    11161 ggatgcgctg cagatcgaaa acctgatgga caacgaactg gaaacgcatc accacgaagg
    11221 ggcggtgccg gtgcatgcgc taagcaagct ggccgacgga ctgccggctt tcggtatcgt
    11281 ggccgcggtc atgggggtgg tgcacaccat ggagtcggtg ggcgctccgc cggccgaact
    11341 gggcatgctg attgcgcatg cgctggtggg tactttcctc ggcattctgc tgtcctatgg
    11401 ttttgttggt ccgctggccg ggttgctgga acagaaactc gacgaatcga gcaagatgct
    11461 gcattgcgtg aagacgaccc tgctggccag cctgaacggg tttgcgcctt cgcttgcggt
    11521 cgagttcggt cgcaaggtcc tgtactcgac cgagcgtccc ggttttgccg agctggaaga
    11581 gcatgtcaag agaaaatgag gccaagggca ccggctacgg ggtgccgtgc agggtatttt
    11641 tgggggtgaa tggtggctga agcccgtccc atcgtcgtca agcgcatcaa gaaggtggct
    11701 ggtggtcatc acgggggggc atggaagatc gcgtacgccg actttgtcac ggccatgatg
    11761 gcgttttttc tgttgatgtg gctgctgggt tcgacgacaa aaggtgacct gaaaggcatc
    11821 gccgattatt ttgccacgcc actcaaggtg gcgctggaag gcggttctgg cagcggcgac
    11881 agttcgagca tcatcaaggg gggagggaaa gacctgacgc gggattcagg tcaggtctcc
    11941 aaagcatcca accccaagat caagaagacg atcgacctga aggaggccaa agccgatctc
    12001 gaacacatgg atctggagcg cctcaaactg ctcaagtcac agattgaaca tgcgatcgat
    12061 gccaatccgg tgctcaagca gttcaagaag cagatgctgc tcgacatcac taccgaaggg
    12121 ttgcgcatcc agatcgtcga tgaacagaac cggccaatgt tcgcttcggc gcgggctgaa
    12181 ctcgaaccgt ataccaagga tattttgcat gccatcggcc agatgctcaa caacgtgccc
    12241 aaccgtatca gcctgaccgg acacaccgat gcgacgcctt atgccaatgg cggtacgtct
    12301 tacagcaact gggagttgtc gtccgaccgc gccaatgcgg cacggcgtga actgatcgca
    12361 ggtggcatgg acgaaggcaa ggttctgcgt gtggtgggca tggcttcggc cattcctctc
    12421 gacaagagca atccttacga tccggtcaat cggcgcatca gcatcatcgt gctcaacaag
    12481 aaaaccgagg atgctatcgt gcgcgatggc gcggtactca atgccagcaa tggcaaggag
    12541 gtatccgcgg gactgaaagt gaacacagtg gccggcgcag tgagcgttgc gcccggtaaa
    12601 agcgcccagt aggcgctgcc agccgggcga ttcaccggga gccgttttgc tcagccgggt
    12661 gttgtgacaa tgaattcgag attgtcgatg agtcgggtct cgcccagacg cgcagcgccg
    12721 agcacgacca gatcgcgcgt tgccgcttgc ggtggatcga gcctgcggtc acggacgcgg
    12781 atgtagtcgg gctgccagcc tgcggtcgcg agtgcggctt cggcttcgtt ttccaggcgg
    12841 gtcaggtcgt gcaggccatg ccgttgaacc tgcgcggcga cagatgtcag cgtggcgtag
    12901 agttgtgctg cctgttcgcg ttgcccggca ctcaggtact ggttgcgtga cgacagcgcc
    12961 aggccatcgt cggcgcgcag cgtttccccg ccgaggatct cgatcggcac attgaatgct
    13021 tctgccatca gccggatcag cgtcaactgt tggtagtcct ttttaccgaa gcaggccata
    13081 tcgggctgca gcagttgcag cagtttcatc acgatggtgg cgacgccgcg aaaatgtccc
    13141 ggacggctgc ggccgcacag atcctgtgcc agtggcggca attcgatgtg acaggtttgc
    13201 ggtatcggaa acagggtggc tgcatccggg gcaaacagca gcccggtgcc gacagactcg
    13261 agttgttcga tgtctgcggc aagcgtgcgt ggataacggt cgaaatcctc acccgcgcca
    13321 aattgcagcg gattgacaaa aatgctgacg gcaacatgct gcgcatgctg ctgtgccagt
    13381 cgcaccagcg acaggtggcc tgcgtgcaga ttgcccatgg tgggcacgaa gcctagcggg
    13441 ccggtcagtt gccgccgcca ttggcgtagt gcggcgatgt catgtatgac cttcatgtcg
    13501 cagtggtacc gaagctgtgc gcagtggtgg gaaagtcgcc ctgcttgaca gcgcgaacat
    13561 aggctgccag cgccatatcg aggtcacagc cgtcggtcat gaagttttgc gcaaaacgcg
    13621 gtggtttccc cggggtaatg cccagtgcgt catacagcac cagcacctga ccggaacagt
    13681 cggggccggc gccgatgccg atcgtaggcg cagtggtcct ggcggtgatc tggctggcaa
    13741 gcggcgcggg tatggcctcg agcacgatca tgcccgcgcc acactgatcg agctgcactg
    13801 cgtcgctgat cagttgctgc gcggcgtcgt gcgcgcgccc ctgcacccgg taacctccgg
    13861 tggcattcac ggactgcggt tgcagtccga tatggcagca gaccggaatc ccgcgagcgg
    13921 tgaggaaagc cacggtttcg gccatgatcg cgccgccctc gagtttgacg caatgtgcgc
    13981 cagcggccag caggcgcgtg gcagattgca acgcttgcgc cggatgctgc tggtaactgc
    14041 cgaagggcag gtcggcgatg atgaagctgt gacgggcccc gcgtgctact gccgcggtat
    14101 ggtaagccat ttcatccaga ctgaccggca tcgtcgaggt ctggccctgg atgaccatgc
    14161 cgagggagtc gcctaccagt atggcgtcga tgcccgcggc ttccacccgg gcggcgaagc
    14221 ttgcatcgta gcaggtgagc atggccagtt tttcgccatc acgcgcacgc cgttgcaggg
    14281 aaatcaggtc ggacatggct gctctccgtt acagggactg gttgaaaaat tcccgcgggc
    14341 tgcgcatgtt ggcaatatgc tgcatgagca gggcaaaatg actgtcgtcg tcgacgaaat
    14401 tgaagcgctc gcagttgacg atcagaagcg gtgcgcggtc atactggtaa aaatactggg
    14461 tataacggtc cgacagacgg gcgaggtatt cggacccgat ctgtgtttcg tagccaatgc
    14521 cacgtttgcg gatgcgttcc agcagcacgg acggccgtgc ctgcaggtaa atgaccaggt
    14581 ccggtgccgg cgcttgcaag cgcagctcgt tgtagatggt ctggtagagg cggaattcgt
    14641 cgttgtcgag gttgagttcg gcaaacagcg ggtccttgtc gagcaggaaa tcggcaaccc
    14701 gtgcacgatg gaacagatca tgctgacgga tctcgttgac ctgagcactg cgctggaaca
    14761 ggaagaacag ttgcgtggcc agcgcataac gcgccggctc acgatagaag cgggcgagga
    14821 acggattttc gcccgcattc tccagcagca gttcggcgcc gaggtgggtg gcgagccgct
    14881 gactgagact ggtctttcct gccccgatcg ggccttcgac ggcgatgtag ggtgattggt
    14941 cgataaggct catgtagcga tttcgtcctg ttcgagccgg acaatggact gctggccgac
    15001 tgcggccagg aatctgctgg ccgcccccag tcccggaatg acggcatccg gtgtgatttc
    15061 aagcagcggt agcagcacga acgcacgttc gtgcatgcgc ggatgcggca gcgtcagctg
    15121 ttcgtcctgc caggtgagct gattgtacaa caggatgtcg agatcgatga cgcgcggcgc
    15181 attgcgaaag ctgcgctgac gcccgctgtg ctgttcgatg tcaagcaggg ccctcagtag
    15241 tgcaggcggt tcgagacgcg tatgcaatcg tatgacggcg ttgatgaaat cgggctggtc
    15301 ggtatgcccg acggccgcgc tgcgatacag tgaggacacg gccgtgatct gtacgccgga
    15361 aagccgggcg atgttggcga tggctgtgcg gatctgctgg cgtgggtccg acaggttgct
    15421 gccaagcgca acgtggcagg tggcgccgga tggcgtgacg tcaatcatgg gcgggtggcg
    15481 attcgacggg ccggtttgcc gatggcttgc gtcgacgccg gcgtctgggt gccgcagccg
    15541 gcgccagtcc ggcgataagg ttggcgcgca gtgcgtcgtc ggcgctctgg aactgggtcc
    15601 accagtcggc cagatcttgc gctgcttcgc cactttcggc gcgcagcagc aggaagtcat
    15661 aacctgcgcg aaagcgcgga tgcgcgagca gccgccaggc gcgcccgcca gtgcgctggg
    15721 caaaacgcgg ttgcagactc cagatttcct tgatggtgcc gtcgagccgg cgcggaattg
    15781 ccagcgcttc acgctgcttg tcgagaacct cttccatggc agcaaacagt gcaggcattt
    15841 cattctcgcc ggcatcgaga tgggcgcgct ggcgttcgag cacttcgtgc cataacaggg
    15901 cagcaaacag gaatgcaggc gagacaggct tgccggcctg gatgcgcaga tcggtattgt
    15961 gcagcgctgc ggtaaggaag cggctgccgg tttcgtcgcc aagcagcttg tcgagcatcg
    16021 gcagcacgcc gtaatgcagt ccttcggccc gcaactggtg cacggcgcgc agtgcgtgac
    16081 ccgacagcat cagcttgagc atttcgtcga acagccgtga ggggggcaca ttggcgagca
    16141 gttcggccag tgccggcatg ggggcgcgcg cggtctcgtc aatgtggaaa tccagtttgg
    16201 cggcaaagcg cgccgcgcgc agcatacgta cagggtcttc acggtagcgc gtgcccgcgt
    16261 cgccgatgat gcgcaacact ttgttgcgga tgtcctgaac gccaccgcgg taatcccaga
    16321 tttcttcttt gcccggatcg taatacagtg cgttgacggt gaaatcgcgg cgttctgcat
    16381 cgctttcctg ggtgccaaac acattgtcgc gcacgatgcg gccggtgtcg tcggtgacat
    16441 gggcgccttc gccctgttcg cccgctgcgc tggcgcggaa ggtggagact tcgacggttt
    16501 cgttgccgca cagcacgtgt accaggcgga agcgccgacc gatgatgtgt gcgcggcgga
    16561 acagggcgcg gatctgttcc ggcgtggcat cggtgaccac atcgaaatct tttggcgtct
    16621 tgtcaagcag caggtcgcgc actgcgccac cgacgataaa cgccgcatga ccatgctgct
    16681 gcagcgtttc ggtaactttg agcgcgcacg gcgagatgcg ttcgcggggc aggccatgtt
    16741 cgctggcggg aatgcgttgc aggcgcgatt tgcgcggtgc tctggcggtg gagcccagca
    16801 atcgggaaat gaagcgtttg atcatggagg cggtcgtgag gtcaggcacc gtgcagttca
    16861 agcagtggcc agcggttccg ggtggcatgc tcgcgcaata ccggatcggc attgacggca
    16921 accggatggc tgacttgctg cagcagcggc aggtcattgt gcgaatcgct gtagaaccag
    16981 gtttcgtcga aacgttcgaa aggcttgccc tgttcggcca gccactggtg catgcgcgcg
    17041 atcttgcctt cccgaaaact cgggaggccg gatacgcgac cggtgtagcg gccgcccgcc
    17101 tgttccggct cggtggcgat gaggtgctcg acgccgaact cgcgcgcgat cggccgggtg
    17161 acgaagctgt tggtggcggt tatgatggct accagatcgg cctggtcgag tgagcgtgcg
    17221 accagttcgc gcgccgcagt cgtgatcatg ggcgcaatgt gctcgcgcat gaactgcgcg
    17281 tgccaggcgt tgagctgttc cggcgcgtat tgtgccagcg gacgcagctg gtggtcgaga
    17341 aaggcgctga tgtccagcgt gcccagtttg tactgttcgt agaattggcg gttctttgct
    17401 tcgtacgtct gtttgtcgag cacgccctga ctgacgagga actgtcccca ggcataatcg
    17461 gaatcgccag cgagcagggt gttatcgagg tcgaaaagta ccagtttcat gcagttgtcg
    17521 tcgggatctc tggggcgtgt cggcacacgg aaaattgaag tggtggccgg tgccggatgc
    17581 ggctcccggc cgggtatccg ctcatgatgt ccagatgcgc caccaggcgt gcggcggctc
    17641 ggtaatgcca tgcagtacca atggtttctt gtcgtcgagc cagacgctgt gcgggaagtt
    17701 ctgctgcagc acgcgtcggc tgtcgcctga cagattcggg gcgcccatgg cggcgtaggc
    17761 gcgcgtcatg atggccagac ccagctcatt caccggcgcc tgcggatagt gggtcagtgt
    17821 gtcctgggcg cgttcgactg cggcgaccca ggccttgcgg ttgaagtaat acagtgcaat
    17881 gtgctcttcg tgctcggcca gcaggttttc caggaatttc atgcgcagcc gtgcatccgg
    17941 cacgtatttg ctgtcgggat accgggtaac cagttcgcgg aagctgtcaa acgacgcgac
    18001 caggtccttg gtgtcacgtt ccgagatgtc ctggccggca atgcgggaga acgcggtctg
    18061 gtctgacacg aagtttgaca gccctttcag gtaatatgca taatcgacgt ccgggtgatt
    18121 cgggtgcagc ttgatgaagc ggttgcaggc ttcgacggcg gatgcgggct ggtcgtcctt
    18181 gtagtaggca taggccactt caagctgtgc ctgctgggcg taacggccat agggatagcg
    18241 agcctcgatg tcttcaagca gcttgctggc cttgttgtag ttggccgctt tcatttcgtc
    18301 catggcctgc gcgtacaggc gttgggctga ccagtttttg gtttcatcct ctacagcggg
    18361 cttgttggat gcgcaaccgg aaagcacgag cgtcagcagg aggaaagcgc ccgatgccag
    18421 cgccggaatg gacagatgcg gtttcatgac tcgatccgga aaacaattgg agaattatac
    18481 aacaataaag cgggcggacg acggcgtacg ctgtctgcaa attccgttcg atgcggcagg
    18541 cggacggctg gatgctgtgc tggctggtct gttgcccgaa tattcgcgca gtcgcatcca
    18601 gtcgtggatt cgcgatggcg atgtgcggct ggatggtgcg acggctgaag tgcgtacccg
    18661 ggtgcgtggt ggcgaactgg tcgaaatgac ggtcgagccg caaccagcca gcctgccatc
    18721 gttgcccgag gatattccgc tcgatgtggt ctacgaagac gatagcattc tggtgatcga
    18781 caagccggcc ggtctggtgg tgcacccagg cagcggtaac tggcaaggca ccctgcttaa
    18841 cgggttgttg catcatgcgc cacaattgac ccatgttccg cgagcaggta tcgtgcatcg
    18901 gctggataag gacaccagcg ggctgatggt ggtcgccctg acgctgcagg cacagacctc
    18961 gcttgtgcgg cagttgcagg cgcgcacggt gcgacgggag tacctggcta tcgtgcaggg
    19021 ccgggtcggg caggatggcc gggtcgatgc gccaattggt cgacacgcca cacagcgaac
    19081 gcgcatggcg gtggtcagcg gcggacgcga ggcggtgaca cactaccggg tggtgacgcg
    19141 ctttgccgcg cataccctgg tgcgctgttc gctggaaacg ggacgcaccc accagattcg
    19201 cgtgcacatg cagcatattg gccatccact ggtcggcgat ccggtatatc atcgccgccg
    19261 cacggaaccg gtcggatgcg cgcgtgcatt tgaccggcag gcactgcatg cgacgcagct
    19321 cggattgctg catccgctga ccggcgagcc catgctgtgg cagcgtgatc cgcctgccga
    19381 tttcgtggct ctgctgaatt gtctcgcgac gcaggaagcc acaggggagg cgcgaatttc
    19441 gcagaataac aagaatgctg gatgagcagg tgctttgacg gcggatggtc aggaaggaaa
    19501 aacatgtcat ccaattggat ctatcccgat tggcctgccc cggcgcgggt aaaatcgctg
    19561 gtgacgactc gtggcaacgg agtgagccgg ggggtgtatg ccggcctgaa tctgggcgat
    19621 catgtcggcg atgatccgat cgacgtggcg caaaaccgta cgctgctgcg cgagatgctg
    19681 ccgagtgacc cggtgtggct cagacaggtg catggtgcga cggtggctta tgccgaccat
    19741 ctgcgcgaaa ctgttgaggc agacgccgcc gtggcacacc gtaaccagac cgtgtgtgcc
    19801 gtgttgaccg ccgattgcct gccggtgctg ttctgtgcca ccgatggcag cgtggttgcg
    19861 gcagcgcatg ccggctggcg cggcttgctc gccggcgtgc tggaacagac gctggacgtg
    19921 atgggcttgc cggcggagca ggtcatggcg tggatggggc cggcgatcgg gccggaggat
    19981 taccaggtcg gcaacgaggt gcgtcaggca ttcgtttccg actttgccca gtcggctgcc
    20041 ggattcaagg ggtgtggttc gggaaaatgg ctggcagaca tgtacacact ggcgcgtcaa
    20101 cggttgcaac gtgccggggt cacccgcatt tatggcgggg gattcagcac ctatgccgaa
    20161 ccggtgcggt tctactccca ccgtcgcgac ggggtgacag ggcggatggc cagccttgtc
    20221 tggattgacg actgagcgtg gccggttgca ttcagtgacc gtttccttcg ccggactgcc
    20281 ggctggcgcg tctccgcttg cggccggggg tgtcgagaat gtaatagagc aggccgatcg
    20341 ccggcccgcc gagcaatacg agcgagagcg ccgcgccgat ggtcggtgag gtggctatca
    20401 tcatgatcca gatatacagg atggcaatca gtatgatgta catggtttcg tgtcatgagc
    20461 cgcgctaacg gggaacattc cattccgcgc gtgggtttcg tttcattggg ctgcccgaaa
    20521 gcgacttcgg attccgaacg cattttgacg cagttgcgcg cagaaggcta tcagatcgcg
    20581 gcagattatg ccggttcaga cctggtcatc gtcaatacct gcggtttcat cgatgctgcg
    20641 gtcgaggagt cgctcgacgc cattggcgaa gcgctggcgg aaaatggcaa ggtgattgtc
    20701 accggatgtc tgggtgcacg ggaatcggtg gtgcgtgcgg cccatccagc cgtgctggcg
    20761 gtgaccgggc cgcacgacac cgatgcggtg atggctgccg tgcatcggca ttcgccgcgt
    20821 gtgcacgatc cgcgcctcga tctggtgccg ccgcagggca ttcgcctgac cccaccgcat
    20881 tacgcctggc tgaagatttc cgagggatgc aaccaccgct gcacgttctg catcattcct
    20941 ggtttgcgcg gcgacctggt cagccgcccg gtcggagacg tgatgcagga agctgaacgc
    21001 ctggtcgcag cgggcgtgcg cgaactgatg gtgatttcgc aggatacctc ggcctatggc
    21061 gtcgatgtgc gttaccgtac cggattctgg aatggcagac cagttaaaac acgactcgga
    21121 gaactggcgc aggcgttgtc gacgctcggc gtatggattc gcatgcatta tgtatatccc
    21181 tacccgcatg tggacgagtt gattccgctg atggcagaag gcaagattct gccttatctc
    21241 gatatcccgt tccagcatgc cagtccgcgc attttgcgtg ccatgaaacg gccggcggcc
    21301 agcgaaagcg tgctggaacg gattcgttcc tggcgcagcc aatgcccgga actggtgatc
    21361 cgttcgacct tcattaccgg tttccccggc gagacgcagc aggatttcga tcaattgctg
    21421 cagttcatcg acgaggcaca actggatcgt gtcggctgtt ttgcctattc cgctgtcgag
    21481 ggagccgctg ccaatgcgtt accagacccc gtacccgagg ccgtgaagga agagcggcgt
    21541 gcgctgctga tggagcgcca ggccgatatc agtgccgaac ggctggccgc acgcatcgga
    21601 acacgcatga cggtgctggt ggatgaagtg aatgaagacg gcgcgattgc ccgcagctat
    21661 gccgatgcgc ctgaaattga cggtgtggtg gtgatcgaga tgccgggaaa actgcaggca
    21721 ggagacaagg ttgaagtcag catcaccgac agcggtgaat acgacctgtg ggcagaactg
    21781 ctgccctagt catcccatag ccggcgtcag ggcccgctgc atgcgggcgg aacgccgctg
    21841 gtaacttcgg gagagatgcg agcagcgggg tgtgccgggg catcaggccg tcagccgacg
    21901 acatgaccca gcgaaggcgc cgggtggctg gtctgaccgt tgggaccgta aagattgtcg
    21961 agcgacgtga cgccaccgga caggacgccg agtgcggcgc ggttgcggtt gagctgggtg
    22021 tgaatcagca ggccgttggt gcggttgact tccttggccg aacgcacctg acccagcagg
    22081 ttgcgccagg cttcgtcgac ctgcggttgc gggtgacgct ccagccattg ctgcatgccg
    22141 gtctcactgg cggcgcagcc gtgcgcgtca agcagggcat ggcgtctggc ggccagtctg
    22201 gcgagctgtg caacgtgctc ggccttgtgc tggatgaccg gttgcatatc ctgcacgcgg
    22261 gactggacca gcaactcctg ttcttcgcga atcagcccga gcagcgcccc gaccgcacga
    22321 ttctcttcgg ccagatattc gagcagttgc gaatccagcg tggtcatgcc tgctgattgc
    22381 ctcgggattg caacaggctg cgtaccgagc tgatgagctc attggccacc ttgctggtat
    22441 cgacggtgaa ctggccgccg gcgatggcct gtttgatggc ctcgaccttc tgcgtatcaa
    22501 aggcgccgct ggtggcaatg gtggactgca gggcctgcag cggtggcgaa gtcgaggcaa
    22561 tgctgccggc accactgtcg acggaggaaa ccggcgcagc cggagcgttg cgtgacttgc
    22621 ccgcgttggc cgccccgacc gacgggctgg acacgttttt gacggaatca gttatcttca
    22681 tgatgtcatc tctggcgctg gcgcccggaa tcatcgggct acagcatgat tttcggcagg
    22741 attctgcata actttaggaa aatcctgatc aaatgtctgc ctggacaaca ggatgcctgt
    22801 ctgcctgacg gattccggtt gcggcattcc acaagccttg ccggggatct cgcatgcccg
    22861 gtgttcaggg cgttgcacac gggttgccgg caatgcgcgg aacatcccgc tcaccatggg
    22921 cgttatggta gcagaaaacc gtgtgcggca acccggggat tcgcttcctg cacggtctgg
    22981 cggcgtagct ccggccacga cctgcgccta cgggtcgcga tcttcccgat ctggctggca
    23041 acgaggtact tggagaaaca ggcacatcag cagcgtcctg gcgtgtcgcc atgtccggtt
    23101 gcaacaggaa gacggcggtg aacagaaaac ggatttacca gttgaccagc acggtattgc
    23161 cgttgccggc gatgccggta accaccccgc ccccagcaac gcgcacttcg acgcgctgtc
    23221 cggcttcggc cggggtgatg gcctgggctt cggcggagat gtcgaatccg ttgccatggg
    23281 aaacgagctt taccggctga ccaggctgga tggtggtctg tgcgcgcaat acgctggtgc
    23341 gcagcggcgt accggcgggc agtgaaatgg tgagcgcttt gccttccgcc tgactgatat
    23401 ccgtgacgag gtcggatggc aggcgcgtca gatcaccact gaccacggtg aactggctgc
    23461 tttcaacggt ctgtccgcgc gtgagcgcgc cggtgctgac caggtaattg ccatggatgg
    23521 tgatattggc ttgcagatag agcgtccagc gctgtggtgc attgcagcgc acgccgacca
    23581 gggtgtggcc ccacaggcga ctgttgccgg gtagaaatgc ttccgggtgg gcgcatgctg
    23641 ccgcatggat acgttcatcg agtgaatgga cctgcaccgt gactttccct ggaagacggc
    23701 ttgcctgacc gtagaggtag tcctgtgcca ggcgcaccag ggcggccggg tcctgctgcg
    23761 cgggcggttc tgccgcatgc gcgctggcaa ggatgaacag ggtgcacagg gtgaacaacc
    23821 gtttcatggc acaaaggtat ccgcgggccg gggtgatgga gacatgatac ggcgccggtg
    23881 cggatccaat tcggagaata gcctcgggtt tgccggttta ttccgcgttt cgattgacgg
    23941 cggctggtct atgatgaagc ctgtgatgaa tgtgcggagc aacggtcatg gggccactcg
    24001 acaacagcat gcggtttcag gagacggcgc tgcaattgcg cgaacagcgc cagcagattc
    24061 tggcgtccaa tatcgccaat gccgatacgc cgggttacaa ggcgcgcgac ttcaatttca
    24121 gtcaggcctt gcagggagcg ctcgccgggc agccgctcac catgcagggc aatgtggctc
    24181 caggcggtgt gccgctgcaa taccggatac cgcagcattc cagtatcaat ggcaattccg
    24241 ttgatatgga taccgaacgc aaccagttta cagacaatgc cttgcgcgac gaggcgagca
    24301 tgacgctgat caccaaccag atcaagacca tgctggccgc aatacagggg taacgagcca
    24361 tgtccttgtt taccatattc aatgttgccg gttctgccat gagcgcgcag tcgcagcgac
    24421 tgaacgtggt ggccagcaat ctggccaacg ctgacagcgc gaccagcagc accggtcagg
    24481 tgtaccgcgc ccggcaggtg gtgtttcagg ccgttccggt cgatgcgaac aacgcggttg
    24541 gcgtgaaagt gcaacaggtg gtggaagacc cttcgccgcc gcgcatggtg taccagcccg
    24601 acagtccgct tgcggacgcc aaggggtatg tggccatgcc caacgtgaat ccggtcgagg
    24661 aaatggtgaa catgatttcc gcatcgcgtt cctatcagac cgatgcggat gtgatgaatg
    24721 cagccaaggt catgttgcag aaagcattga caatcggcca gtgatgtccg caaccgggat
    24781 ggcaaggaga ataggtgatg agcagtgcga tcaacagcaa taccatttca tcgagcctga
    24841 tggcggcgat gaacggaacg tcctcgacag cgtcggcgac agcctcggga tctgcggtca
    24901 actcgatgca gaaccagttc atggatttgc tggtgacaca gctgaaaaac caggatccgc
    24961 tcaatccgat ggacaatgcg cagatcacca gccagcttgc gcagctgtcg accgtgacag
    25021 gcatcaatca gctcaatacc acgctgcagt cgctgatgtc ggcgtaccag tcggatcaga
    25081 ctgtccaggc ggccaacatg atcggcaaga acgtgctggt caacggttcg acactgaatt
    25141 acaccggcgg cacggctacc ttcggcgtca atctggcgtc ttcggccgac agcatgacgg
    25201 tgaacatcgt caattcgggc gggcagacgg tggacagttt caatctggga tcccagcctg
    25261 ccggcgtggt gccattgcag tgggacggca cgaccagcaa tggcacggcc gcgccgaacg
    25321 gcagttacac catccaggtg aaggccagca gttcggggac agccgtcgga gcccagccac
    25381 tggcgtatgg tacggtgtcg agtgtactca acaacggtag cggaacacag atcgatgtga
    25441 atggtgtcgg gatggtcgcg ctgtccagcg tggcgcagat ttattgatcg ggaattggaa
    25501 agcgggagat catcatgagt ttcgatacag cactgagcgg attgtcggca gcctcgcagg
    25561 acctggacgt gatcggtaac aacgttgcca actcgggtac cgtaggttac aagcaatcgc
    25621 agatccagtt cgccgatatt tatgccaatt ctctggccga cagcgcttcg ctgcaggtgg
    25681 gtaccggcac caaggtcgca caggtggagc agcagttcgt gcagggcaac atcacggcct
    25741 ccaacaatcc gatggacgtg gccatcaacg gccagggttt tttcgtcatg gacagcggcg
    25801 gcagcacggt ctatacccgt aatggtcagt ttcagctcaa caagaacggc aatatcgtca
    25861 atgccaatgg tgacatcctc aagggttatc cgctcagttc gaacggacag atcaccacgg
    25921 gagcactgag cgatctggtc atcaattctt ccaacatggc tccgagcgcc actaccaaaa
    25981 tcaacgccgg cctcaatctt gacgcaacca gtaccgcccc ggcgaccgcg ttcaacacca
    26041 ctgacccgac gacctacaac aacagcacgt cgacgaccat ctatgacagt ctgggcaatt
    26101 cgcatgtgct gcagacctac tatgtgacca atggcaccgg ttcgtggaac gtgtatgcct
    26161 cgcttgacgg tacgccggtg gggtatacgc caccggccac agccgtgccg gtgtcgacac
    26221 tgaccttttc gagcagcggc gcgctgtcag gcggttcccc ggtgtcgttg agcttgccgt
    26281 tgaccgatgg cgcgagtaca ccgatgtcgg tcagtctcaa ttacaccggc accacgcagt
    26341 acggatcggt gttcggcgtg aattcgatgc agcagaacgg ctatacctcg ggccagctga
    26401 gcaacttcac catcggtagc gatggcatga ttaccggcaa ttacaccaat agccagtcga
    26461 ccacgctggg tcaggtggcg ctggcgaatt ttgccgatcc caatggcttg caggcgatcg
    26521 gcaacaacga atggatcagc acctatgcct ctggccctgc gctggcgggc gtgcctggta
    26581 gcgcttcgct gggggtgctg cagtcgtcgg ccacggaaag ctcgaacgtc gatctgacca
    26641 agcaactggt ggacatgatt acggcgcagc gcgattacca ggccaatgca cagaccatca
    26701 agaccgaaga cgccgtcacg cagacgctgc tcagcctgcg ttaacggaac attgatttcg
    26761 ggagaacgtc atggataacc tgatctacac ggcaatgacc ggcgcaagcc atgtgctgga
    26821 gatgcagtcg acggtatcca acaatctggc caatgtggca accaccgggt tccgtgcgca
    26881 gttcgacgcg ttccgcgcgg tgccggtagt caatggcggc ctgccggtgc agacgtacgt
    26941 gaccgattcg accgtaggca gcagttttac tcccgggccc atgatacaga ccggaaacaa
    27001 cctggatgtg gccgtgcaaa gcaagggctg gatcgcggtg gacatgggca acggcactga
    27061 aggctatacg cgtaatggca gcctgaaaat cgactcggcc gggcagctgc agacctggaa
    27121 tggcctggcg gtgcagggcg tcaatggccc gattaccgtg ccgcccggcg agcaggtgca
    27181 gatcggttcg gatggcacgg tgtcgagcat tccgcccaac ggcgtcatga cctcggtcag
    27241 cgtgctgggg cagatcaagc tggtcaatcc gccagattct gcactggtgc gtggcgatga
    27301 tggctatttt cgcctggcca gcggcaaggc ggccaatgcc gatccgactg tggtgctggc
    27361 ctcgggtacg cttgaaggca gcaatgtcag cgcagtatcg gaaatggtca gcatgatcgg
    27421 cctgagccgc cagtttgaaa tgcagatgaa gctgcttacc aatgcggaaa acaacgatag
    27481 ccaggccagt cagtttctga ccattgcctg acgaaaaaca aggagcacga catgatccgt
    27541 tcattgtgga ttgcccgtac cgggatggat gcccagcagt tgcagctgga cgtgatatcc
    27601 aacaacctgg ccaacgtcag caccaacggc ttcaaggcgt cgcgtgccgt gttcgccgat
    27661 ctgctctatc agacccagat gcaaccgggc gcccagtcga cgcaacagac ccagttgcct
    27721 tccggattgc aggtcggtac cggcgtacag ccggtggcga ccgaacatat cctgacccag
    27781 ggcaacctgc agcagaccgg caattccact gatcttgcca ttcagggtgc gggctatttc
    27841 cagatcacgt tgcctgacgg cacgacgggc tactcgcgtg acggatcatt ccaggtcaac
    27901 aaccaggggc agttggtcaa ctcgctcggc tatccgctgc aaccgccggt gacgatccct
    27961 tccaatgcgc tgtcggtcac gattggtgtc gacggcacgg tgtcgatcac ccagaccggt
    28021 tcgcccaatg cggtgcagat cggttccatc cagttggcaa ctttcatcaa tccgggcgga
    28081 ctgcagagca acggtggcaa catctatctc gaaacctcat cctcgggtgc acccatcacc
    28141 aacacgcccg ggctcaacgg cacgggtacg atcaaccagg gctatgtcga gacgtcgaac
    28201 gtcaatgtgg ccgaggaact ggtgaacatg atcgagaccc agcgcgccta tgagatggac
    28261 agcaaggcga ttacggcctc cgatcagatg ctgcaggcac tgacacagat cagctgacgc
    28321 gggagagtga cgatgaagcg cgcaattctt gtgttgtgtc tcggtatgct gggcgggtgt
    28381 gccgtggtgc cggagacgat cacgaagcag cctgcaactg cgcatccagc gccacaagtc
    28441 gccgccagcg gggatggctc catttatcat gcaggaacat accggccgat gtttgttgac
    28501 tggcatccgg tgatggtggg tgatgtgatc accattctga tcagtgaaac caatgatgct
    28561 tccaagaccg cttccagttc cggcagcaag accggttcgg tctcggccag cattccgact
    28621 gtagtaggac tgccgctcaa gacgctgcaa ggcaccaatc ttgcggccaa caccagcaac
    28681 aaggcggcca ccaccgacgt gctcaattcg gccaacacat ttaccagcac actggcggta
    28741 acagtgactg ccgttcgccc caatggggat ctggaagtca gcggcgaaaa acaggtggcg
    28801 ctggacagcg gcgtggaata catccgtttt tccggtgtgg tcaatccggc catgatccag
    28861 gctggcaatg tggtgtcatc gacccaggtg gccgaggcgc gcatcgaata ccgttccgac
    28921 agccgcatcg acaaggcatc gctgatgtcg gcgctgagcc ggttcttcca gagcatggct
    28981 ccgttctgac ggaacaggga gacaatcatg aaacattacc tgacgatact ggcgatgatg
    29041 ttgttgctgg cgatgaatgc ggcccgtgcc gatcgtctgc tcgatctcgc cagcattcag
    29101 ggcgtgcgcg ataaccagct ggtcggttac ggattggtga tcgggctgga cggtaccggt
    29161 gatcagacgg tacagacgcc atttacggtg cagagcatca ccgacatgct gaccaacatg
    29221 gggttgagtg ttccggccag ccagctgccg atgctgatgt tgaagaatgt ggctgcggtg
    29281 atggtaacgg cgaatttgcc ggccttcgct caggtgggac agaaaatcga tgttaccgtg
    29341 tcatccatcg gcaacgccaa gagtctgctg ggcggtacgt tggtgatgac gccactcaag
    29401 ggcgccgatg gtcaggtgta tgccatggcg caaggtaacc tgctggtggg ggggatgggc
    29461 gcgtcagcca acggcagcaa ggtgcaggtc aaccacctga cggtgggact cattaccgat
    29521 ggcgccgatg tcgaacgggt ggtgccttct gctgtggggc aggggaacgt ggtgcgcgtg
    29581 gaactcaatc agactgattt tgccacggcc agccgggtgg tgaatgccat caatgcgcgg
    29641 tttggcatgg atgttgccac cgcccaggac gggcgcgtga tcgcggtgca ggcgccgatc
    29701 aactccaatg aacgcgtggc gttcctgggt acactggaaa gtatccaggt cgatccttcc
    29761 caggaggggg cgcgcgtaat catcaatgcg cgtactggct ccgtggtgat gaaccggaat
    29821 gtcacgctcg atccctgcgc catttcacac ggtaacctga ccattaccat cagcagcacg
    29881 ccgtcagtca gtcaacccaa tcccatgtcc gggggcaaga cggtggttac cagtaccgcg
    29941 gccatttccg tgcagggcgc gggcgagccg ggttcggtag acctgctgaa gggtggggca
    30001 tcgctggccg aggtggtgcg tgcgctcaat tcggtgggcg cgacaccaca ggacctgctg
    30061 gccatcctgc aggcgatgaa gacggcgggc gcattgcacg ccgatctgca gatcatttga
    30121 ggccgccatg ctcaatcgcg ccgacatcag taacactctg gctgccgatt cgcagggcgt
    30181 ggccagactg cgccaggcgg cgcaacagaa ttcgccgcag gcgttgcgta cggtcgcgca
    30241 tcagttcgag gcgcttttta ccgaaatgat gctgaaaagc atgcgcgatg ccacgccgca
    30301 ggatggcgtg ttcgataatg atcagacccg gttgtatacc tcgatgctcg atcagcagtt
    30361 gagccagaac cttgccaaca agggcatcgg gctggccgac atgctggtgc ggcagttgtc
    30421 acccagcagc ccgcatcccg ctggccggtt tgtgctgccg cattcgccgg gaactggcac
    30481 ggcggcgcaa ccggcagcac aagattcgcc agcagtatcc ggcggcgccc cggcaacggg
    30541 gccgggtgcg cccacatccg gtcgggagca tgcctttatg aaggccatgc atacgcatgc
    30601 cgaggctgca agccgagcca gcggcattcc ggccagattc atgctgggtc aggcggcgct
    30661 tgaaagcggc tggggcagca aggaggtggt cggcaccgat ggcacgcgca gccacaacct
    30721 gttcggtatc aaggcaggtg cagactggca tggaaaaacc gttaaagcgg tgacaacgga
    30781 gtatgtcaat ggcgtggcac aaaagcgtgt tgcaacgttt cgcgcgtata acagttatgc
    30841 tgacagtttc aacgattacg ccagttttct gcgcagcaat ccgcgttacc gtcaggtgct
    30901 tgccggggcg caaagtccgg agcagtttgc acgagggttg caacaggccg ggtatgccac
    30961 tgatccgcac tatgccgaca aactcggacg gattctcagc agcccgatcc tggcttgata
    31021 ttcgacggga ccacccgacg tgcctgaggt gcgccggtta aagttatctg cctgacggac
    31081 gataaatccg acaagcaccc aggacattac gccatggcta tctcaagcat tctcaatatc
    31141 ggtcaaagcg cgctggcggc ggcggaaatc ggattgaccg tcaccgggaa caacattgcc
    31201 aatgcttcga caccaggcta cagccgtgag cagacggtac agaccgaagc cactccgcaa
    31261 ggattcggtt acggtttcgt cggttccggg gtggatgtgt ctaccattca gcgccagttt
    31321 tccagttttg ccgcccaaca ggtcaattct gcacaggcca gttcgagcaa tctgtcgacc
    31381 tattacacgc agatgcagca gatcgacaac atgctcggcg acacgaccac cggtctgacc
    31441 ccgaccatgc agagtttctt ttccggggtg cagaccctgg cgaccacgcc gtcatcgttg
    31501 tcgagccgtc aggcgctgtt gtcgcaagcg caggccatga cctcgcagtt ccagagcatg
    31561 gggcagcagc tcgatcagat cggccagggg gtcaacacac agatccagac cagcgtgagt
    31621 tcgatcaacg ccatcgccag ccagatcgcc cagctcaatg gcagtatcgt ccaggcacag
    31681 gacgcttcgc agcagcagcc ggccaatgcg ctgctggatc agcgcgacca gctggtcaac
    31741 cagctcagcc agcagatcaa tgcatcggtg gtgcaggaat ccaacggcca gtatgatatc
    31801 tatatcggaa acggtcaggc actggtgatg ggacagaacg tcaacacgct gtccaccgtg
    31861 agctctccgc tcaattcgag ccagctgagc gtggcattca cctcgaacgg ttcgacggcc
    31921 actttgcccg acagcgccct gaccggcgga cagcttgggg gggtactgtc gtttcgtgac
    31981 cagtcgctca acagcgcgca gaaccagctc ggactgctcg ccatcggaat gggaacgaca
    32041 ttcaacgcac agagtgcgct cggacaagac ctgaatggca atccgggcac caagtttttc
    32101 aatgtgggct cgccgcaggt ctacggtgcg tccaccaaca ccggttcggg tgtgctgacg
    32161 gcgagcatca gcaatgtgaa tgcactgacc gggcaggatt atcagctgac cgataacggc
    32221 accagttata cgctgacccc ggtgaatggc ggcgcgtcga ccacgttcac cagctttccg
    32281 cagacgattg ccggggtgac tttcaacatg agtggctcgc cggccacggg cgatacgttt
    32341 ctggtccggc cgaccgccag cggcgcggac aacttcagtg tggcgatcac cgatccggcg
    32401 cagattgccg ctgcagcgcc gatcgtgacc tcgacggtgt ccgatccgcc ggtgatgaca
    32461 gccagtgctg tcggcaatac gggtagcggg gcgatcagtg cggcggacat cggcagtaat
    32521 ccgacctcgc cgctgactgg cccggtgacg ctgacttata acggtactgg caataccctc
    32581 agcggctttc cggcgtcgca acctgtcacg gtcaccacgg ccgtcggatc gaccaccacc
    32641 tatccggccg gaacgccggt accctattcc gatggcgcga ccatcagctt tcagggcatc
    32701 agtttcaata tcagcggcac accggccaac ggtgacagct tcactctgag caacggcggt
    32761 actggccaga tcagtccggg cagcgtcagt tcggcttatc tggcaacgcc gttgaccgca
    32821 ccgcttactt tgacctacag ttcggcaagc aattcgctgt cgggttttcc tgccgcggat
    32881 gcggtgacgg tgaccactgc cagtggtacc accacgtatc cggccggtac gccagtgacc
    32941 tataccgacg gcgcgacgat cagtttcggg ggcatcagct ttgccatcag cggcacgccg
    33001 gtcaacaatg acacgtttac tgtcggaccc aataccggcg gcaccgggga tagccgcaat
    33061 gccgtgctgc tcggacagtt gcagaacacc aatacgctgc tcaatggcac agccaactat
    33121 cagaatgtct attccagcct ggtcagcagt gtggccagca agaccagtga gctgcaggtg
    33181 accagtactg ccgaaaatac cactttgacc aacctgcaga acgtgcagca gtcccagtcc
    33241 ggggtcaatc tgaacgagga agccaacaat ctgctgcaat atcagcaggc gtatcaggct
    33301 gccggaaaac tgatgcaaat cgccagccag ttgttcaatt cgctgctggg catcagtaat
    33361 tgaggaggat gacatgcgca tcagcacaaa catgcttttt tcgcagggcg gtacgcagat
    33421 cagcaacctg caaagccagg tgaccacaac ccaggcggaa atttctgccg gcaccagctt
    33481 gctgaccccg gcggataacc cggcagctgc ggcgcaatcg atggatgttt atcagcagca
    33541 gtcgatgaac acccagttta cctccaaccg ccagatggct gaaaccacgc tcaacatgac
    33601 gggttcggcg ctgtcgagca tgactacgtt gctgcgaagc gtgaagtcgc tggtggtcag
    33661 tgccggcgat cccacactca ccgctgctga ccgcagcaac attgccaccc agatccagaa
    33721 tgactacagc agcctgctgg cgttggcaaa ttccactgat ggcaacggta actacctgtt
    33781 cggcggctac aagaacagta cgccaccgtt tgtgcagacc acgggcgctt cgggtacacc
    33841 atcggtgcag tacctggggg atcaggggca gaccgcgctg caggtcggtc ccagccagca
    33901 ggtgacggtc agcacgtccg gacaggccat gctgcaatcc ggtggacagg acatttttgc
    33961 cacgctgagc accctgacca gtctgcttaa caactcgaac gtcagcaatg caaccctgac
    34021 gcagggactg tcggggataa atgacagcat cacccagacg ctcaacaagg tgatcaatgc
    34081 cagcagcgtg gcg
//
LOCUS       DAIANL010000006         4286 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1026183, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000006 DAIANL010000000
VERSION     DAIANL010000006.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026183"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          839 a         1274 c         1308 g          865 t
ORIGIN      
        1 cagcagcagc cacaccccgg accagtatgc gcgttttgcc cgtttcgcgc gcgacgtcgg
       61 tctgctgtcg tcgcgcggtt ccgactttca ctctcccgaa gaaacctacc gtgacctggg
      121 tcggttaccg tcgctgccgg cgctgtgcgt cccggtatgg cacgactggg aggctgtctg
      181 atggcgcaat atttcacggt gcatcccgac aatccgcaac cgcggctgat tcgccaggcc
      241 gctgaaattt tccgcagcgg cgggttggtc gcattgccga ccgattccag ctacgcgatc
      301 acctgccgcc tcggcgagaa gactgcacag gaacgcatcc gtcagctgcg cgatgtggac
      361 gagcggcatc atttcacgtt gctgtgccgc gatctgtcgg aaatcggcgt ctatgcccag
      421 gtcgacaacc ggcaattccg tctgctcaag gccaatacgc cgggcaacta cacattcatt
      481 ctcgaggcca ctcgcgaggt gccgcgccga ttgcaacatc cccgtcgcaa cacgataggc
      541 ttgcgcatcc ccgcgcatcc ggtaacattg gcggtgctgg cggaattggg ctaacctttg
      601 ctcggcatga cgctcatcct tccgggtgaa tccgttccga tgagcgatcc gctggatatt
      661 cgcgatcgcc ttgaacggca gatcgatctg gtgcttgacg ccggatcctg cggcatcgaa
      721 ccgacgaccg ttctcgacct gagcggcggc ggcatcgagg tgctgcggcg tgggcggggc
      781 tcgctggcac ctttcggact ggaatgaatg gaactcaatc tcgtacagaa aatttcaatc
      841 tgggtcatcc cggtcatttt cgccattacc ctgcatgaag ctgcccacgg ctatgtggca
      901 cgctatttcg gtgacaagag cgcatggatg ctggggcggg tcacactcaa tccgctgcgg
      961 cacatcgacc cggttggcac catcctggtt ccgctgacca ttctgttgat gagccgcctg
     1021 ctcggcgggg gcttcatcct gttcggttgg gccaaaccgg tgccggtaaa cttttccgcg
     1081 ctgcgcaatc ccaagaagga catgctgtgg gttgcgctgg ccggcccggc cgccaacctg
     1141 ttcatggcat tgtgctgggc gttcatcatc cgcttcggtg tcatgctcgg cgacagctac
     1201 agtcagccga tcgtgctgat gggagcggca gggattttca tcaatgccgt gctgatggcg
     1261 ctcaatctgc tgccgctgcc gccgttggat ggcggccgta tcgtcaccag tctgttgccg
     1321 gcgcgccagg catggaactt tgcccgaatc gagccttatg ggttgtatat cctgctgttt
     1381 ttcatggcaa tgggctggct caacgtgctg ctgagcccgg tcatggcact gactctggtg
     1441 ctgaccggcg cagtcgccgg cattggcaac agtgatctcg tttacctcat tcaggcaatt
     1501 accagctagt catgtacgca aatcgagttt tatcgggcat gcgcccgacc ggcaatctgc
     1561 acctggggca ttatcatggg gtcctgaaga actggatcaa gctgcagcat gagcatgaat
     1621 gcctgttttt cgttgccgac tggcacgcgc tcaccacgca ctacgacaat ccggacgtga
     1681 tccgcaacaa cacctgggaa atgatcatcg actggctggc cgccggcgtt gatcctgcgc
     1741 tatcgacgct gttcattcag tcgcaggtgc cggaacacgc cgaattgcac ctgctgctat
     1801 cgatgttcac gcctctgggc tggctggaac gggtgcccac ttacaaggac cagcaggaaa
     1861 agctggtgga aaaggatctg tccacctatg gctttcttgg ctacccgctg ctgcaaagcg
     1921 ctgacatcct gatctatcgc gccaacctgg tgcccgtcgg cgaagaccag gtgccgcata
     1981 tcgagtttac gcgtgagatt gcccgtcggt tcaaccacct gtatgggcgt gaagccgggt
     2041 ttgagcagaa agccgaggct gcgatcagga aaatgggtgc caaaaaggca cggctatacg
     2101 aggaattgcg cacgcgctat caggagcaag gcgaggaaga tgcgctcgaa gccgcgcgtg
     2161 cattgctcaa cgaacagcaa aacctctcgc ttggtgaccg cgaacgtctg ttcggctatc
     2221 tggaaggtgg cggcaagatg attctggtcg agccacaagc gctgctgacg gaagcatcgc
     2281 gtatgcccgg actggacgga cagaaaatgt ccaagtctta tcacaacacc attgcgttgc
     2341 gcgaatcacc cgaagaagtc aaccgcaaga tccgcaccat gccgaccgat ccggcgcgca
     2401 tccgtcgcac cgatccgggc acgccggaaa aatgcccggt ctgggcgctg caccagatct
     2461 actccgacaa ttcggtacgg gagtgggtag tgcagggctg caccagcgcc ggcattggct
     2521 gcatcgaatg caagcagccg gtgatcgatg gcgtggtggc cgaactcgaa ccgatgtggg
     2581 aacgggcgcg tcattacgcc gaatcgccgt cactggtacg caacctgatt gccgacgggt
     2641 gcgaacgcgc ccgggaactc gcccgcgata ccatgcgcga tgtgcgcgag gtcatgggtc
     2701 tgaatcacaa ctgatccgcg cccgttgcgg agcgcggcaa tgcagggcga gggttcagta
     2761 gccagtgttg ccgtgcacgt cgggcagctg ggcatcatcg gagaagaacg accacagcag
     2821 gcgcgccatg acttccggcg cgatgacggt atggtggttg tccagttcgc gcaggcggtc
     2881 gcgcagttgc tggcggaatt gcagattgcc ggtcaggtcg aaaatgatgt ccacgccgtc
     2941 accgagctcg gcgatcttca gtccgtcgct gtagacgggc accttgctgt gggtaaagcc
     3001 catggcgacc ggggaatcga tattgtgggc cgcaaccgcg acgatctcgg cggcaatatg
     3061 gcgaatctgg atgctggcaa gaaaatgctc ggcgaaggtt tcaccgaccc ggcccaaacc
     3121 aatcagcgcg atgcgaatgt tttttgtcat gttgtgtcct cctgttgttt tacctgaaca
     3181 cggtactgcc gtgctcgtgc tgcgacatgc gacaatgcgt ctgtcacaac cgtgcagcct
     3241 acacccgaat cttttcgcag acaatccatt ctcatgagcc gaagcagaat tcagtacaac
     3301 tgcaccgaat gcggtggcat ttcagtcaaa tggcaaggac agtgcccgca ttgccaggcc
     3361 tggaacacgc tggttgaaac tgctgcggaa tcgtccgcac catcccgtca caatgcctgg
     3421 tcggccacca gtcgcatcga gcggctggaa caggtcgaat tcaccgaaga aacacgcatc
     3481 gccagcggca tcgccgaatt cgaccgtgtg ctcggcggcg gactcgtgcc gggaggcgtg
     3541 gtcctgatcg gcggcgaccc gggtatcggc aaatcgacac tgttactgca gacgctggcg
     3601 gcgctggcat cgaaccatgc cacgctgtac gtcagtgccg aggaatcggc gcagcagatc
     3661 gccatgcgcg cccggcggct gcaattgcag gggggggcga tgcaactgct ggccgaaatc
     3721 cgtctcgatg cgatacttga gacgctgcgg acgcagcatc cggcggtggc tgtcatcgat
     3781 tccatccaga cggtgtactg ggacgaactg caatcggcgc cgggctcggt gagccaggtg
     3841 cgcgaatgcg ccgcgcagtt gacgcgccat gccaagctga gcggcaccac gctgtttctt
     3901 gtcggtcatg tcaccaagga aggcgcattg gcaggtccac gcgtactgga acacatcgtg
     3961 gataccgtac tgtatttcga aggcgacagc agttcgagct ttcgcctggt gcgcgctttc
     4021 aagaaccgtt tcggcgcagt caatgagctg ggtgtgtttg ccatgaccga ccgtggcctg
     4081 aaaggcgtaa acaacccttc ggcgctgttc ctctccggac agcgtaccgg cgtacccggg
     4141 tcatgcgtca tggtcacaca ggagggtacc cgaccgctgc tggtggaaat ccaggccctg
     4201 gtcgaaaatg cggccagccc caacccgcgt cgactgtcgg ttggcctcga acagaatcgt
     4261 ctggcaatgc tgctggcggt gctgca
//
LOCUS       DAIANL010000007         5962 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3070484, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000007 DAIANL010000000
VERSION     DAIANL010000007.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3070484"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1122 a         2024 c         1760 g         1056 t
ORIGIN      
        1 tgaaggcgtt gttcagcgtc cgcccgcgca tgtaagccag cggcgcgatt tcgatgacgc
       61 cacgctcggt catgcgcgcc atcttttcgc tgcccatcag atcatagagc gcgtcgtaca
      121 gcggccgcag gtagggatcg atcttctgcg ccaggtcgcc cggcaggaaa ccaagccgct
      181 cgccagcctc gaccgccggc cgcgtcagga tcagccgctt gaccagatcg cgttccagcg
      241 catctaccgc gcttgcaacg gccaggtaag tcttgccggt tcctgcagga ccgatgccaa
      301 aggtaatgtc atgcgactgg atctgcgcca ggtagctgtt ctgccgcggt gtgcggcccc
      361 gcagttcgcc tttgcgcgtc tgcaacgcaa gaaacggctg ttcgccgaca ttgtgcgccg
      421 attcaaccag cgccagctgg atatcctcga ccggaatcgg caccgtagcc tggcggtaaa
      481 agcgttcaag caactgcgtg gcccgacgca cactgtccgc ggccccggac acattgaaat
      541 gctcaccgcg gcgtgcgatt gtcacgccca gcgccgtctc gatctggcgc aggttttctt
      601 ccagcacgcc acacaggttg gtcaggcgga cattatctac ggggacgaac gacagctggc
      661 tcacgatatc cgctcgccgc gcagcaggtg ggcgcgtgcc tcggtaatgc gcacgtcgac
      721 aaactggccg atgcattgtg gatctgcgac gaaattgacg atgcggttgt tggcgctgcg
      781 cccaatgagc tcgctgccgt cacgtcgcgc caccccctcg accagaacac gctgcaccgt
      841 tcccagcatg cgctgaccaa cagcctcgac acagcgtccg agcgcttgct gcacccggat
      901 cagccgctgc tggcgcacct cgctgtccac ctcatcgttc aggtcggcag caggcgtgcc
      961 cggacgcctg ctataggcaa aggaatatga aatatcgaaa ccgatgtcat cgatcaggcg
     1021 caacgtcgcg gcaatatcct cttcggtctc accggggaag cccacgatga aatccgaaga
     1081 gatgctgatg tccggccgcg cctcgcgcag gcggcgcacg atcgacttgt attcgagcac
     1141 ggtgtaacca cgcttcatcg ctgccagcac gcggtcggaa cccgactgca ccggcagatg
     1201 caggtgggcg gcaagcttcg gcaaccggcc gtgggcttcg atcagacggg gggtgcattc
     1261 acgcggatgg ctggttgtgt agcgaatgcg ctcgataccc ggaatttcgt gcacgtgttc
     1321 gagcaacagg gcaaagtccg cctgttcgcc gtcagcgagc aagccgcggt aggcattgac
     1381 gttctgcccg agcagcgtca cttcgcgtac accctggcgc gccagcccgg tcacttcggc
     1441 cagcacgtca tcaaacgccc gcgaaacctc ctccccgcgc gtataaggca ccacgcagaa
     1501 ggtgcaatac ttggaacacc cttccatgat ggatacgaac gcactcaccc cgtcactgcg
     1561 gggcgcgggc agatggtcga atttctctac ttccggaaac gacaggtcaa gctgcggacg
     1621 acccgtggca cggcggcggg cgatgagttc cggcaaccgg tgcagcgtct gtggcccgaa
     1681 caccacatcg acaaacggcg cgcgcgccac aatggccgca ccttcctggc tggccacgca
     1741 gccacccacg ccgatcacca gctgaggatt ggacgccttg taggcgcgta cccggcccag
     1801 gtcggagaac accttttcct gcgctttctc gcgcaccgaa caggtattga acaggattac
     1861 atcggcctgc gcagggtcat ctgtcggctg caaaccttcc gcctcgcgca gcacgtcggc
     1921 catacgcgcc gaatcgtact cgttcatctg gcagccaaac gtcttgatgt agaccttttt
     1981 cacgacagcg tcatccacgg gaaagcgcgc cgttcaaggc tgggcgcggc cagccggctg
     2041 gccgccaaga tcgcgcactt cggcctgcac ttccagcgtt tgtgcatggt gtccggcctc
     2101 atttaccacc aacgcgagcg gaatgcgttc accggcatgc aattgccggc gcaggtcgag
     2161 cagcatgaca tgcagaccgc ctggcgcaag ccgcaccgtc tggccggcag gcagatcgat
     2221 gcgcgccacc gggcgcatgc gcatcacgcc attgtccatg ctcatgcgat gcagttgcgc
     2281 actcccggcc accgggctac tggcagccac cagggcggca ttctgttcgc tgcgtatcgt
     2341 gaggaacacg ccggccacgc tctggccggg cgccggctga cgcagccaga catcctgcac
     2401 cgagacctca gccgcctgag ccgctgtcat gagacacaca ccggcgaccg ccggcgcaag
     2461 ctggcgcagc cagcgaaacg aaaccctgct gtcagtgcga gggcggaaca taaccgttca
     2521 ccctgtcacc accgtcgccg aagaaatact gttcaacctg ttccatcagg tacttgcgag
     2581 cacgggcatc ggcaaggttg agccggtttt cgttgaccag catggtctgc tggcgcagcc
     2641 agcccgccca ggcttccttg gacacattgg cgaagatgcg ctgccccatc tcgcccggca
     2701 gcggtggaaa atccagccct tcagcttcac gccccaactt gatgcaattg accattcttg
     2761 ccataccatt ttcctttcaa gcttgcatga tgacctgcgg ctcgcccgca tgcgccacga
     2821 tttccccgat gcgccagacc gtttcacccg ctgcagccag ttgctgttgc gcgcgcgcag
     2881 catcggcggc acgcacaacc agcaccattc cgataccaca attgaaggtg cggtgcatct
     2941 ctgcatcggc aatgttaccc tgctgctgca accaggcaaa caacggcggt tgcggccagc
     3001 tgtgcggatc gatgcgcgca gcggttccgg ccggcaggat gcgcggcaca ttgcctgtca
     3061 gtccaccccc ggtgatgtgc gccatgcccc ccacctgcat ctgcgccagc aatgccagca
     3121 gcggacgcac gtaaatgcgc gtgggcgcca gcagtgcatc tgccagtgtt gcatcaccga
     3181 aacggctgtg cagatcaaca cccgagcgat cgacgatctt gcgtaccagc gaatagccgt
     3241 tggagtgcgc accgctcgac gccagcccga gcaccacgtc gcccggcgcg atgcggctgc
     3301 cgtcgagtat ggcgctgcgc tcgaccacgc cgaccgcgaa gccggccaga tcatactcgc
     3361 cgtccggata catgccaggc atttccgcag tctcgccgcc gatcagcgca cagccagcct
     3421 gctcgcaacc ggcagcgatg ccacgcacca ccgcttccgc ggtgtcgaca tcgagcttgc
     3481 cgcaggcaaa ataatcgagg aagaacagcg gctccgcccc ctgaacgaga atatcgttga
     3541 cgctcatcgc caccagatcg atgccgacag tgtcgtgacg gtcgagctga aaagccaatt
     3601 tgagcttggt gccgacgcca tcggttcccg agaccagcac cggttcgcgg taccgttgcg
     3661 gcaactgcat cagcgcaccg aacccgccta tcccgcccag cacttccgga cgcatggtcc
     3721 gttttgccca gggcttgatg cgctcgacca gactgtcgcc ggcatcgatg tcgacgccag
     3781 cgtcgcggta ggaaagggaa tcgggtggtg ttgccgttgt catcgcgagc ctggttctgt
     3841 tcaatgatcc gatattctac cgcaattcgg tcgccacgtt cattaccggg cgccgtacag
     3901 gtataatgcc gcatcaattt gtccggccgg aacgcagcat gaacgatgcc atgtatgaat
     3961 cccgcattcc ttcgcttcga ctcctgtcgc gcgggaaagt gcgcgacctg tacgaagtcg
     4021 acgaacaccg cctgctgatg gtcgccaccg accggctgtc ggcattcgac gtcatcctgc
     4081 ctaccccgat tccgggaaaa ggcaagctgc tcacggcgat ctcgctgttc tggttcgaca
     4141 agctggcggc gctgttgccc aaccagctga ccggcgacgc tgccgaatcg gtggtgctgc
     4201 cggaagaacg cgaccaggtg gccgggcgcg cgctggtggt caagcggctc gaacccctgc
     4261 cggtcgaggc tgtggtgcgt ggctacctgg caggttcggg gtggaaggaa tatctcgcgc
     4321 acggcagcgt gtgcggcgta gcgttgccgg ctggacttca gcttgcgcaa cagttgccgc
     4381 aagccatttt caccccatcg accaaagccg ccgtcgggga acacgacgaa aacatcgact
     4441 atgcccgcac cgaacacctg ctcggtgcac aacgcgccgc agaagtgcgc gacgccagcc
     4501 tcgcgctgta ccagacggcc gcagactggg cccgccagca tggcattctc atcgccgaca
     4561 ccaagtttga attcggcgtc gaccgcaacg gcacgctgca cctgattgac gaagtgctta
     4621 cccccgactc ctcgcgcttc tggcctgccg accaataccg gcccggaacc aacccgccca
     4681 gttttgacaa gcagttcgtg cgcgactggc ttgaagcctc gggctggaac aagtgcccac
     4741 cggccccgca gctgccgcag accattgcac tcaaaactgc ggagaaatac cgcgaagctg
     4801 ccgccgcgct gcttggcagt gttcgtgcct cggcgctgct gggcaacgct caggttccgg
     4861 gcgactgagc agcgtcaatc cccagttcct ggatcttgcg cgtaagcgtg ttgcgcccga
     4921 gaccaagcag ggtggcggcc tcaatgcgcc gcccaccggt atgcgatagc gccacctcga
     4981 tcaacgtttt ttcgaacgcc gcggtcaacg tatccataat gccagtctcc ccgcgcctga
     5041 gcgctgcacc ggcggtttgc gacaacgcct gcatccagtc gctcgctgat tcgaccgccg
     5101 gcacggactg cagaatttcc tgcggcagat cggagacatc gacgttgttg cccggcgcca
     5161 tgatcgtaag ccagtggcac acgttttcca gttggcgcac gttgccgctc cagcggtact
     5221 ggctcaggta acgcatggcc gcctcggaca gccgcttggg ctccaccccc tgctcctgcg
     5281 cgctcttgag caggaaatgc cgggccaaca gcggaatatc ctcgttgcgc tcgcgcagcg
     5341 gcggcagacg caggcggatc acattgaggc ggtggaacag gtcttcacgg aacaatcctg
     5401 ccttgacgcg cgcttccaga tcctggtgcg tggcggcaat gacgcgcaca ttgaccttga
     5461 ccggctggtg cccgccgacg cgatagaact ggccgtcggc cagcacccgc agcagccgcg
     5521 tctgcagttc ggctggcatg tcaccaattt cgtcaagaaa cagggtgccg ccgtcggcct
     5581 gctcgaagcg cccgcgccgc tgtccggccg caccggtaaa cgcaccccgt tcatggccga
     5641 acagctccga ttcgagcaag tcacgcggga tggccgcagt gttgagcgca atgaacggcc
     5701 tggctgcacg caggctgtgc cgatgcagcg ccgacgctac cagttccttg ccggtgcccg
     5761 actcgccggt gatcagcacc gtggcattgg actgcgacag acgcccgatg gcacgaaaca
     5821 cctcctgcat cgccggcgcc tgacccagca tctccggcac gccctgcagg tgcaccggat
     5881 cgctttcgtc gttgctgcgc aggctttcgt cgatggcgcg ccggatcagt tcgaccgcat
     5941 gatccacatc gaatggcttg gg
//
LOCUS       DAIANL010000008        13212 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2867424, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000008 DAIANL010000000
VERSION     DAIANL010000008.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2867424"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2245 a         3786 c         4569 g         2612 t
ORIGIN      
        1 ccgcgttcta tcgcgatttc gagtggatgg gtgtgcagcg acacctcaag gtgctgggca
       61 ttttttcccg cttgtgccac cgcgatggca agcccggtta cctggccgac atgccgcggg
      121 tgctcgagca tgtgcgcaag accgtgttgc gttattccga gctggcgccg ctggcgggtc
      181 tgctcgatac ctggcatggc gaacgggtgc aggacggatt gactttttga agacggccat
      241 gattcttgcc gccggacggg gcgaacgcat gcggccgctt accgacgtga cgcccaagcc
      301 cctgctgcct gtcggggggc atccgctcat cgtgtggctg ttgcggtcgc tggccgcagc
      361 cggctttgaa cgcgttgtga tcaatgtcgc ccatctgggg gaacagatcg aagccgaact
      421 tggcagcggc gcggcttacg gcgtggaaat ctgctggtcg cgggaattgc cggcgctgga
      481 gacggccggt ggcattgcca gggcattgcc ctggtttgac ggaaatccat tcgtggtggt
      541 taatggcgat ctgtggaccg attacgattt cgccgctttg cggcgcaggg ggctgccgca
      601 cggtcggctt gcccacctgg tgctggtgga caacccgcag caccacctgc agggtgattt
      661 tggtctggag ccggacgtca gttcgctgtc cggcagcagc gtgagcgaac aggcgcagcc
      721 ccgactcact ttttccggca tcggctgcta tcatcccgac ctgtttgccg aagtggcaac
      781 cagccggccc gtgccgctgg ggccgttgct gcgcacggcc atgcgcgccg gtgccgtcag
      841 tggcgagcat tttcgtgggc gctggcacga catcggcacc ccgcaacgga tgctgcaact
      901 tgatgcgcag ttgcgcactc tgggagttga aaatggatat tgaggccgtg cgccaggcgg
      961 tggccatcga tcccgagcgt caggacagtc tgcaacacag ctggctggca ctgatggacg
     1021 tgtcgctctg gggcagggtc aagtcctccc gcctcggagc aatgagccgg ttgcgacgtc
     1081 gcgtgctgga cgtcggcgag aaactggtat cgctgtgcgc cgtacgcgac tggattccgc
     1141 acccgcgcga gcagctgaag aatgcgctcg gttcatcgct cgccctcaag gatgccctgg
     1201 cccagctgca tcgtgccgcg gacgacatcg acggcggcga ggatggcgag catttttcca
     1261 cgcagcttgc tgcgctggaa caggaactcg cggcctggct gcctgaactg gagaaccgct
     1321 gggcaatgct gctggatacg caatatgaag agccggaaga tgatgacgca tcctgatctg
     1381 gcagtgtatg ccgcgcgtcg cgcacgactg atggccgagc tgggctccga tgcggtcgcc
     1441 gtgctggcta ctgcgccgga acggctgcgc aatcgggaca gtcattatcc ataccggttt
     1501 gacagtcact tttattatct gacggggttc gccgagccgg aagccgtgct ggtgctgcat
     1561 ggtgcgagcg ggcgttcgat cctgttctgc cgcgaacgcg atcccgaacg cgaaatctgg
     1621 gatggcttgc gttatggacc gcaagctgcg cgcgaggcat tcggtttcga cgaagcctgg
     1681 ccgatcggcg aactcgacca gcgtctgccg gactggctgt ccggtcaggc ggcgctgcac
     1741 tgcgtgctgg gtgaagacgc ggcctgggac cggcgtgtgc tggactggct gcaggcgttg
     1801 cgcgcactgg cccgcaacgg ggtgcaggcg ccacggggca tgttcgatct gcggcgcgtg
     1861 ctcgatgcaa tgcggctcat caaggacgag cacgaaatcg ccatcatgca gcgctcggcg
     1921 gatatcgccg ctgcggcgca ctgtcgtgcc atgcgcgcca cccggcctgg catgaacgaa
     1981 tatcagatcg aggcggaact gctgtacgaa ttccgtcgac agggtgcgca ggcgccagcc
     2041 tatacgtcga tagtcgccgg cggcgcgcat gcctgcgtgc tgcattatgt cgagaataac
     2101 ggctggcttg ccgacggcga gctgctgctt gtcgatgccg gttgcgaact ggatggctat
     2161 gcctcggata tcacgcgcac gtttccggtc aatggacgtt tcaccggtgc ggctcgcgat
     2221 gtctatgcct gcgtgctcgc cgcacaggcc gccgcgctgg agcaggtgcg gcctggcaac
     2281 agctggcagg caccacatga agccgccgtg cgcgtgttgg cgcaaggcat gctcgactgg
     2341 cgactgttgc aaggttcgct ggacggtgtg gtcgaatcgg gtgcctacaa gcggttctac
     2401 atgcaccgca ccggtcattg gctggggctt gacgtgcatg atgtgggcga atacaagcag
     2461 gggggcgaat ggcggccact gcagccgggc atggtactca cggttgagcc gggatgctat
     2521 atccgggcag cggaggacat tccgcaggcg ttttgtaaca ttggcgtgcg tatcgaggat
     2581 gacgtgctgg tcgacgttga tggtcatcgg gtgctgacca gtgcggtgcc cagcgcgatg
     2641 gcaggcatcg aggcgttgat ggcccaggcc ggcgaggaac gcgaacatgc gtgaacatgt
     2701 cgatgtgacg attgtcggtg gtggcccggt gggcgcctcg ctggcactgg cgctggcagg
     2761 cagcgggctg gatgtgcagg tgctcgaggc gcgtgccagc ctgggccagg cggcgtggcg
     2821 gcgcacgctg gcgttgtctt acggcagccg actcattctc gaccggctcg atgtctggtc
     2881 tgccctgcgt gacgtgacgc cgatccgtca cattcatgtt tcgcaacgcg gcagttttgg
     2941 cgtgacccga ctgcatgccg acgaagaagg cgtgcccgaa ctcggttatg tggtcgatta
     3001 tggcgaactg gacgccgcac tgcatgcagc gcttgccagc agcggcgtgc gggtggtgac
     3061 cggcacacgt gtcacggact cgggggggat gtccggctac gcctggatcg aagcgcagca
     3121 gaacggccgg acgcgtatga ctacggcccg gctggcggtc atcgctgacg gcggcgcggg
     3181 caacggcaat caggtgacgc gtgaatacgg ccagcatgcc ctgctggcat gggtgcggtg
     3241 cagtgcgccg cgtccgggtt gggcattcga gcgctttact gcgcaggggc cggtggcgtt
     3301 gctgccgcag ggcgaggggt atgcgctggt ctggaccgag gcgccggaac gggtgcaggc
     3361 cctgctcgcc atggcggagc cggagttcct cgccgcgctg cacgaacatt ttggcgatag
     3421 ggtgggcgag ttcctgcagg tgcgcgaccg cagcagcttt ccgcttgtgc tggcctgggc
     3481 gcccgatgcc gtgggcgcgc accggatcat gatcggtaat gccgcccacg tcatgcatcc
     3541 ggtggccggg cagggattca acctcggctt gcgcgatgcc tgggaactgg cggcgcagat
     3601 ccgctccgcc gaccggcagg cgcttggcag cgaagccatg ttgcggcgtt tcgccagcac
     3661 gcgtgcattc gatgtgtcgg ccagcatgtg gtttaccgac cggctggtgc gcctgttcac
     3721 ccggaccgat ccggtgctgc atcatgcacg tggcgccggg ctggccatgc tgcagcagtt
     3781 cgcgccactc aggcattttg tcgcccgtcg catgatgttc ggagcgcggg catggtgaag
     3841 catgatttcg acgtggtcat cgttggcgcc ggtatcgtcg gcgcagcgct ggcggcggct
     3901 ctgcgcggca gtggcctgga actggcgctg gtggagcagc ggccgccgga ggaaccctcg
     3961 tcgcagtggg atgcgcggat ttacgccttc agcccgggca gcgttgcatt gctcgacagc
     4021 gtgggcgtct ggtcgcaact ggatgccggg cgcatcgcac cggtacgtgc gatggatgtg
     4081 cgtggcgacc ggggcgggca tgtgctgctt gatgcctacg aggccggcgt gccgcaactg
     4141 gcgtggattg ccgaaagcgg gcgggtgcag catggtgtgt ggcaggcgct gcgcaatcag
     4201 ccggacctga cgctgttcgc gggtgtgcag gggcgcgacc tgcgttggtc gtctgctgga
     4261 gcgcgactgg aactcgacaa tggcgagcag atcagcgcac ggctggtggt ggcggcggat
     4321 ggtcgggggt cgtgggtgcg cggccaggcc gggatcggcg cgcatcgtgg cgattaccat
     4381 cagtcggggg tggtggcgaa tttcgcctgc acgcgggctc atgaaaatat tgcgcgccag
     4441 tggttccgcg aagatggcat tcttgcatgg ctgccgctgc cgggacacac attgtcgatg
     4501 gtgtggtcga ccgacgccgt gcgcacggcc gaactgctgg ccttgtcgcc ggaggggctg
     4561 gccgaacgtg tggccgaagc cggtggccac gtattgggcg atctgcgtgt gttgacgccg
     4621 gcggcggctt ttccgctcag catcctgcgt gtggacagtc tggtggcacc aggactcgcg
     4681 ctgatcggcg acgccgccca cggcgtgcat ccgctttccg gtcagggcgt caatctgggg
     4741 ctgcgcgatg tcgcccggct ggcccgtgtg ctgcgtgagc gtggcgaagc cggctgcggc
     4801 gacctgcccg tgctgcggcg ctatgaacgg gcgcggcgtg gcgatattct ggcgatgcag
     4861 actgtaacca ataccctgta tcatctgttc ggtgccaggc atccgttgct ggcgcgcctg
     4921 cgcaataccg gtatggatgt ggtgcagcaa ctggcgccgc tcaaatcgcg cctgatgcag
     4981 caggcgctgg cctgaccggt tgcccggcgg gatggatatt tcaagcatgg aaggagcagg
     5041 aatgaaaggt agtcgttggc tgttggcagc cctgatgtgc gcgtgggtgg ggtttgcgca
     5101 cgccgacgag gcatcgattc gcaagaatct gggagcgcgt ttccctggca tggaggtgag
     5161 cagcgtgacc aaaacgccct atgccggcct gtacgagatc gttgtggacg gtcagattgc
     5221 ctatgtcgat gaaaacgccg attacctgtt tctcggcagc gtggtagaca ccaaggcgca
     5281 caagaatctg actcaggatc ggctggcaaa actggacgcg gtcaatccgg atgcgctgcc
     5341 gctcgatcag gcgatcaaga ttgtcaaggg tgacggcagt cgcaaactga ttgtgttttc
     5401 cgatccggaa tgcccattct gtaaacggct ggaacaggag ctgaccaagg tcaacaacct
     5461 gacgatgtat gtgttcctct ttccgatcga gagcctgcat ccgggcacta catcgctgtc
     5521 gcgcgccatc tggtgcgcca aggaccgtgc ccaggcctgg cacgacgccc tgctgaagaa
     5581 tgtccagccc aggaacgacg gcagttgcag caatccggtg gatgcagata ttgcgctggg
     5641 caagagcctg cacgtcagtg gtacgccgac cctgattttt gctgatgggc ggcgtgtggc
     5701 cggggcgatg cctgccgaca agatcgaggc cgcgttcagg cttgcccgac ctgcaggcga
     5761 caagcccgga gcagacaaga ccaaggttgc tccggcgcaa tgatgcgcgg cggatgttct
     5821 tcgccagtca cccggtgacc gggtcgaaca gttgttccag cgccttgccc ggatcttctg
     5881 cgcgcatgaa cgcttcaccg accagaaagg caggaacgcc ggcgccgaac aggcattcga
     5941 cgtcggcgcg gcggtgaatg ccgctttcgg tgatcaccat ccggctggca tcgatatcgg
     6001 ccatcaagtc gagcgtggta tcgatgcggg tttcaaacgt tctcaggtta cggttgttga
     6061 tgccgatgag cggggtgcgc aatgtcaagg cctgcgccag ttcgccgcgg tcatgcgact
     6121 cgaccagcac cgccagccca agctcgtacg cctgcgcttc cagttcacgc atgtgaaccg
     6181 gatcgagcgc ggcgacgatg agcaggatgc aatccgcccc catggcgcgc gcctcgacga
     6241 cctggtattc gtcgatgatg aaatccttgc gcagcacagg gagctcgcaa gcagcgcgcg
     6301 cttcgaccag cgcttcggta ctgccctgga aatagtcacg gtcagtcagc accgacaggc
     6361 aggctgcacc gtgcgcggca tagctgcggg cgatcccggc tgggtcgaaa tgggcgcgca
     6421 gcaggccctt ggacggactg gcgcgcttga tttcggcgat caccgccggc tggccggcgg
     6481 cctgtcgtga cagcagtgca gcgctgaaat tgcgcgaagg tgtctggctg gcggcgctat
     6541 cgcgcaatgc ggccagagac gtgcgttgcc gcgcctgcgc gatttcctcg cgtttggtat
     6601 ccagtatgcg ttcgagaata tcagacatgg tggctgaatg cgacgagttg ttcgagtttt
     6661 ttccaggctg cgccggaatc gatatgttgc agggcaatgt gtacgccttg cgccagtgag
     6721 gtgctgagtc cgctgacgtg gatggcggct ccggcattga gtgcaacgat ttcgctggcg
     6781 gagcgttgct ggccggtgag tgcgtgcgcg aacagttcgc gcgcgtcttc gacactttcc
     6841 acacgcagcg caaccatctc ggcgcgctgc aaaccgaact gttctggcgt gacctgatat
     6901 tcctgcacca cgccgtcgcg cagttcggcg acgtggcttg cacctgacag gctgagttcg
     6961 tccatgccgc ctgcgccgtg aaccacgagc acgtggcggc tgccgagctg ttgcagtgcc
     7021 tgggccagcg gtgcggtcag gtgtgccgaa taaacgccga tcacctggtt ggcggcgccg
     7081 gccggattgg tcagcggccc gagcaggttg aacagtgtgc gcaccccgag ttcacgccgc
     7141 actgggccgg catgtttcat ggcgctgtgg tgattgggtg caaacatgaa gccgataccg
     7201 atttcgcgca cgcagcgcgc cacctgttcg ggtttgagtt gtacattgac gccgagcgct
     7261 tcgagtacgt cggcactgcc cgaagtcgag gataccgaac gtccgccatg tttggcgaca
     7321 cgtacgccgg cagcggcgga aaccagcgca gcagcggtgg agatgttgaa tgtgtgcgct
     7381 ccgtcgccac cggtgccgca ggtgtcgacc agatccggca gttcgtccag tggcacgcgc
     7441 gtggacagcg agcgcatcac ctgggccgcc gcagcgattt ccgtcaccgt ctcacccttg
     7501 cagcgcagcg cgatcagtgc cgcggcgatc tgtgccgggg tgagcgcgcc acccatgatg
     7561 gcatgcatca gttcgaacat ttgcgcgtgt tcgagatcat ggccatcgag catccggttg
     7621 agcagggcag ccaggttcat gcggcctgct ccgtcgaggc gttctggcgc aggaagttgg
     7681 ccagcatctc gtggccgtat tcggtcagaa tcgattcggg atggaactgc accccttcga
     7741 ccgccagcgt cttgtgtcgt acccccatga tttcgtcgtc gtcggtccat gcggtaactt
     7801 cgaggcagtc gggcaggctg tcgcgctcga tgaccagcga atggtagcgc gtggcgcgca
     7861 gcgggttcgg caaaccggca aatacgccac gatccagatg gtggatcatc gaggttttgc
     7921 cgtgcatcag ttcgcgggca ccgacgatgc ggccgccgaa tgcccgaccg atgctctggt
     7981 ggccgaggca gacgcccagc agcggaatct tgcctgcgca ggcgcgaatc agttccaccg
     8041 agattccggc ctggtccgga gtgcatgggc ccggcgagat caccacgtat tgcggtgcga
     8101 gcgcgagcgc ctgctcgaca ctgattgcat cgttgcggaa cacttccacc gtttccccca
     8161 gttcgccgaa atactgcacc aggttgtagg tgaatgaatc gtaattgtcg atcatcagaa
     8221 gcatggtgtg cccattcctc gttgttgtgt cgctcgctgg ccggacggtt gcaacgcctg
     8281 cggcaggtcg gcgcggcgtt gtgcgcatcc ctgacgcatg ccctgaatgg aggtgtgcgc
     8341 caggctgtgt tcgcgacgtg ttccgatggc ctgttgcgga ttgcatgtca atgcatgtcc
     8401 gtgtcagtgc cgggacagcg caaatcggat ggatggagcc tgaaaggcgt cattatccag
     8461 caggcgtcgc aagtattcaa ggcgcgcagc cgggtggagg cgcatcctgg cggcgatttt
     8521 tttcaattgg ttgtaatttt ttcatcatga ttcccattta atatgagtgc tgcatccaca
     8581 gccaactgtg gactggcgga taccctcaag gagaacggca tggcggcggg tctgtttttg
     8641 ctcggtctgg tggcgatcgt ggtgtatttc attccggcgg cagtcgggtt tgcacggcgc
     8701 aaggaaaacc gttttgccat ctttctgctc aatctgtttc tcggttggtc gctgctgggc
     8761 tgggtggtcg cgcttgtgtg ggcagtgtcg aaagacaggc tgccgcaggc ggtgatcgtg
     8821 caggccggca gcgagcgacc gtgaagtccg ttgtcgttca gtccggttcg ccgccggtca
     8881 gtccggcatc ggccagttcg gcggcgcgca ggaccgcgcg ggccttgttg cgggtttcgg
     8941 tccattcgct ttcgggtacc gaatcggcaa cgatgccggc gccggcctgg acatgcagcc
     9001 tcccatcctt gaccagcgcc gtgcggatgg caatggcgag atccatgttg ccgtcaaagc
     9061 ccaggtaacc gactgcgccc gcatagatgc cgcgcttgac ggtttcgagt tcgtcgatga
     9121 tttccatggc acggatcttg ggcgcaccgg agatggtgcc tgcgggaaac gtggcgcgaa
     9181 gcacgtccat ggcccccaac cctggccgca gacggccttc gacgttggag acgatgtgca
     9241 tgacatgcga gtagcgctcg atttgcatgc gttcggtgac acgtacgctg ccggtctggc
     9301 tgacgcggcc ggtgtcgttg cggccgagat cgagcagttg caggtgctcg gcgcactcct
     9361 tggggtcggc aagcagttct tcggcaagtt cgaggtcatg ttcgtcgtcg tggccgcggg
     9421 ggcgggtgcc ggcaattggt cgcagcgtga tggtgccatc ttcgagacgt accaggattt
     9481 ccggcgaggc gccgacgatg tggaaatcgc ccatgtcgta gtagaacatg taaggcgagg
     9541 gattgaggct gcgcaacgca cggtacaagg tgagcgggct ggcatgaaag tcgcgtgaca
     9601 tgcgctggct aagcaccacc tgcatgatgt cgcctgcgtg gatgtattgt tgagcccgca
     9661 gtacggcggc cttgtaggcg ttttcgtcca tcgaggactc gaccgtcgcg cccggaacgg
     9721 gtgcggtgtc cggagtctgc aggggttgtc gtagctgctg cgccagttcg tccagtcggc
     9781 gcgtgccgtg cgcatgggct tgaggatcat tcgggtcgac atagacgatg agcttgagca
     9841 ggccactcag gttgtcgacc acgaccagct cttcggtcag cagcaacagg cagtcggggg
     9901 tatgcaggtc gtcgggacgc gcgccttgcg ccaggcggcg ctcgatgtag cgcacggtgt
     9961 catagccgaa atagccgcac aagccgccag caaagcgtgg cgcgttttcc ggttcggcaa
    10021 catgatagcg ctggaggaag ccggagacga aggtcagtgg atcgtcgctg gttgtctgct
    10081 cgatcaccgc gccgtcgcat tcgacacgga tgtcatggcc gttgatgcgc aggcaggtgc
    10141 gtgccggcag tccgatgaac gagtagcggc cgaagcgttc accgccctgt accgattcga
    10201 gcagaaagga caggggggcg ttggccagtt taaggtagat ggaaagcggc gtgtcgagat
    10261 cggccggcag tacgcgaacg agggcggcgc ggttatagcc ggcttgcgcc agcgcgtgca
    10321 gttgttgagg ggtcatgctt cgggctcctg cgatacgcga ccctcgcctg gcgaaggccg
    10381 caattcaaat gtggtgtaga gtgattgcga cagcggctat cgccatcgcc acgacacctg
    10441 caggaagcgg aaggaagcat gcatcggtca accctttgcg atcagcgttg tggcctgcag
    10501 caggctgtcg acgactgcat ccggatgcag gtcggccacg ggcttgccgc ggttgtagcc
    10561 ataaggcacg cagaataccg ggcatccggc cgcgcgggcc gcctgggtgt cgttcagcga
    10621 atcgccgatc agcaacagtt tgtctggcgt cagcccgaaa tgtttgcagg cgtgctgcag
    10681 cggcagcggg cttggtttgc gttcgggaag gctgtcgcct gaaagcacga gttcgaaaaa
    10741 gtccagcagt ccggtgtcac gcagcagcgg gagcgtgaaa cactcggcct tgttggtgat
    10801 gcaggcgagg tgcagtccca tttcgcgcat gcgcgtcagg ccgtccacga caccggggaa
    10861 tggtcgggat tcgcgtgata cgttttcgct gtagtgccgt tcgtaaatgg cataggcgtg
    10921 gcgaaacagt tcggcatcgg gttcgccgtc catttcgccg gtgagcgcgc gtttcaccag
    10981 gcgcgagaca ccgttgccga tccaggtttc aatggctgcg cgcgacggaa ccggtcggcc
    11041 gagttcggtc atcatttgcg cggtggcgta ggcgagatcg ggtgcggtgt tcagcagcgt
    11101 cccgtccagg tcgataaccg cagcttgtat atgtagcggg aactccatca ggcgaccttt
    11161 gcaagttccg cacgcagagc cgccacgatg ctgtcgtaat ggtgcgcatc cgagtcacgg
    11221 ccagcgccaa ataccgcgga gccggcgacg aatacgtcgg ccccggcacg ggcgacctcg
    11281 gcgatgttgt ccgttttgac cccgccgtcg acttccagcc agatgtcgcg gccggacgcg
    11341 tcgatgcgtt cacgcacggc gcggatcttg ttcagcgttt caggaatgaa tttctgtccg
    11401 ccaaaaccgg ggttgaccga catgagcagg atcatgtcga gcttgtccat cacgtggtcg
    11461 aggtagttca gcggtgtggc cgggttgaac accagaccgg ccttgcagcc ggaatcacgg
    11521 atcagcgcca ggctgcggtc gatgtgttcc gaggcttccg ggtgaaaagt gatgatgttg
    11581 gcgccggcct tggcgaaatc ggggataatg cggtcaaccg gtttcaccat gaggtgcaca
    11641 tcgatgatgg catcggtaac cgggcgcagg gattcgcaca ccagtgggcc gatggtcaga
    11701 ttgggaacat aatgattgtc cattacgtcg aagtgaatga tatctgcgcc cgaagcgatc
    11761 acatcggtga cttcttgtcc caatttggcg aagtttgcag atagaatact gggggcgatg
    11821 cggaatgttc tggacacgac ggctccttgg gtggatctgc tcagggtgta tgtgaaaaaa
    11881 cgattttacc tgaattgtgc tgcgatggca gcacgtactg gatggaatac atcatggcgg
    11941 accaacgcaa acatcacgtg accattacgg tgcaaaccgc gtatttgcct gagcagtctg
    12001 atccggctca ggagcgctac gtctttgcct acacgatcac gatcgtcaat actggctcga
    12061 ttgccgcgca gttgatctcg cgccactgga tcattaccga tgccgaaaac cgggtgcagg
    12121 aagtgcgggg gttgggcgtg gtgggcgaac agcccttcct caaaccgggt gaggcatttg
    12181 aatataccag cggtaccgcg ctcaacactc cggtcggcac gatgcgcggc agttaccaga
    12241 tggtggccga ggatggtgtt catttcgatg cggaaatcgc ctcgttcgtc ctgtcgatgc
    12301 cgcgcacgct gcactgaacc ggcgagaacg tcgtccgacg gctggcgcag tcgattcgct
    12361 gtcgtcccgc cccatgtcac ctgcctatcg tgaacccgag ttgtcgccgg cggcccggcg
    12421 catcgttggc gcattgttgc tgtcgctgag tgcgcacgct gcatttctcg gcctggttgg
    12481 tttgcgcaac ggtgggccgg gcaccacgca gcaggatgcg ccgctgatgc aggtgcggct
    12541 gggtgaccgg ccgcagttgc gcacgacgcc ccccagtgtt gcatctgcta cgcatacgga
    12601 gagcgccatc gccgccgctg tacctgtggt caagcggtcg gccggaacgg ggggtgcgtc
    12661 tgcgccactg gcggtgatcg atgctgcgcc ggttgcagac cccgtgttct acactacgcg
    12721 gcaactcgac gtgctgcccc ggccggtgat gccgatcgtg cccagatatc cgccgatgct
    12781 gaccagcacc agtgtcagtg gctgggtatt gctgcggctc aggcttgacg aggccggcag
    12841 ggtgctgtcg gtgcaggtgc gtgcctccga gccggccggg gtgttcgacc ggtcagcgca
    12901 gcaggcgttt tccggtgcgc gtttcgagcc tggtcgcaag gacggtcgtg cagtgcgcag
    12961 ccagatcgag atcaagatat ggtatggcaa gccctgatgc gcgcgacaga cagtatcctg
    13021 cctgctagaa tgccggatga agctggtcag gcaatcgctg ccccgggtaa ccgggggaga
    13081 ggaaagtccg ggctccacag agcaggatgc cggttaatgg ccggccaccg tgaggtgagg
    13141 aacagggcca cagagacgag tatgccgcaa ggcggacgtg aaacgcggta acctccatcc
    13201 ggagcaaggc ca
//
LOCUS       DAIANL010000009         6569 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1438270, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000009 DAIANL010000000
VERSION     DAIANL010000009.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438270"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1400 a         2150 c         1742 g         1277 t
ORIGIN      
        1 ttgctgcttt ttatctaagc tccgtcagcc agaactccag tcccagccaa cttccccgca
       61 gcaaatgccc actcttattg gtcgtcgttt tgttaaagag cttcttcgct tgcatcggcg
      121 gcttgcaccg ttgcagcaag atgaggtgcg cattataccg gttgaaaaac ggccgtcaat
      181 cgttttgtta aaaaaagatc aaaaaactgt cacacaccat atgaagcaca cgaattccca
      241 tatcgggctg accgtacaat tttcaaccgg caagccggat ccgcgcaaac ttgcgtttgc
      301 cgacctgaat gatccattcg ccaccgggct gaagtttcaa tgcgcgatcg catactttct
      361 caccattgat gcgcaccccc ccctggtcga tcatgcgcag cgcttcggaa gtgctggcgc
      421 acaaccctgc ctgcttcatg acctgaactg ccgaattgcc gccaggctcg cccgccagct
      481 caagcaatgg caggtcgtcc ggcacgcccc cctggcggaa acgcgactcg aaatcctgca
      541 gcgcacgctc agccgcagcg cggtcgtgga aacgctcgac gatttcctgc gcaaaccgta
      601 ccttgatatc gcggggattg atgccctcga ccaccgaccg cttccaccgt tcaatctctt
      661 ctatcgtggc aaacgacaac aggtcgatgt accgccacat gagatcatca gaaatcgaca
      721 tcagcttgcc aaaaatctgt tcgggcgctt cggaaatacc cacatagtta ccgagagact
      781 tggacatttt ctgcacccca tccagccctt caagcagcgg catggtcaga atgcattgcg
      841 gcgcctggcc atgatgcttt tgcagctcac gccccatcag cagattgaat ttctgatcgg
      901 tgccgcccag ctcaagatcc gcgcgcaagg ccaccgagtc gtacccctgc accaaagggt
      961 agaggaactc gtggatcgcg atcggctgat tattgcgata acgcttgctg aagtcatccc
     1021 gttcgagcat gcgcgcgaca gtatgctgcg cagcaagccg gatcatgccc gacgacccca
     1081 attgttccat ccagctcgaa ttgaacacca cctcggtgcg ttgcggatca agaatcttga
     1141 acacctggtc acgataggat tgggcgtttt ccagcacctg ctctttggac aacggcggtc
     1201 gcgtggcgtt tttgccggtt gggtcgccga tcatgccggt gaagtcgcct ataagaaaaa
     1261 agatctgatg ccccagttcc tgcaactgcc gcaacttgtt aagcagcacg gtatgcccga
     1321 ggtgcaggtc cggcgcagtg ggatcaaacc ctgccttgac ccgcaacggc ttgtccccgg
     1381 ccagtttttc cgccagttcc tgctccaccg tgagctcgtc acatccccgc ttgataatgc
     1441 gcaaagaatc ctgcactgag ccttgcatgc ccgtttcctt cctgatgttg tggcaacccg
     1501 atcgccgacc gtggcgcaaa ctgctaggca ttggccgatc ccccatgtta aacttggcga
     1561 ccggattcaa gctttggcaa cgatgatcga tcacgaatca aaaaccggca ttctaccgca
     1621 tggcagcgca gctcaggcac gaaaaaagcg cttgcgatgg ctggtcatcg caagcagtgt
     1681 tcctctgctg ggtatcctga ccgccttcgg tatcgcacca ggtaccgata ccacgcagat
     1741 tcccgtcaag accgtcattg agcaactcga actgcccggt acacctgaca gcatcgacgc
     1801 tcccgccgac accaccggct actggcacaa cgaacgcatt gaacgcggcg acaccgccgc
     1861 cagcgtactg caaagactgg gcgtgaatga cgagcagatc gcccgttttc tcaagtcacc
     1921 cgctgccaca cacgccctga tccagattcg tccaggccag cgcatacagg ccgaagtcac
     1981 cggctccggt cggctgatct ggctgcgcca cctgctttcg aacagccaga tgctcgaaat
     2041 cacgcccggc aaagcgggct tcgaggcaaa tcggcaacag gttgcgcttg aaacccacac
     2101 catcatgaag tcgggtgtca tccgggattc actgtttggc gccaccgatg ccgcgggcat
     2161 tcccgatgcg atcgccatgc agatcgctga catcttctcc acggatatcg attttcacca
     2221 ggatctgcgc cacggcgacc gcttcaatgt gatctacgag atgatgtacc acaacggcga
     2281 accggtcatg gcaggacgtg tgctggccgc agaattcatc aacgccggcc acagttaccg
     2341 tgcggtcctg tccgatgtcg aaggtggaaa accggaatac tacacacccg atggcaagcc
     2401 gatgaaaaag gcttttctgc gttctccgct accatttagc cgcatcagtt cagggtttgc
     2461 catgcgattc cacccgatcc tgaaaaaatg gaaacagcac aagggcatcg actatgctgc
     2521 accgatcggg accaaggtca tggcgattgc cgacgcgacc gtagccttcg tcggccagga
     2581 acggggctat ggcaacatca tcgtgctgca gcatgctggc gcatacagca ccgcctacgg
     2641 ccatctgtcc gcatttgcca aggggctcca ccgtggcgaa catgtctcgc agggtcagat
     2701 cattggttac gtcggcatga ccggctgggc gaccggacca catctgcatt atgaattccg
     2761 tgtcgcaggc atccccacca acccgctgac agcaaaaatg ccttccgcgt tccccattgc
     2821 ggcccagcgc atgccccgct tccgccagat gacgcaaccg ctggtgtcac aactcgaact
     2881 gctgcacgac accaacctgg ttgcgctcga ataacttgcc tgaccccgcg cgccttggct
     2941 gagaccacat gccttatcgg cttgatgtca ggcaccagcc tggatggtgt cgatgccgtg
     3001 ctggtcgaat tcggcccccg gtttccagcc ggtcagcaac tcgccagcca ctacctgccc
     3061 taccccgcag acttgcgcac ccggctgcta aacctgcacc agccgggcta cgacgaactg
     3121 caccgcgccg ccggccttgc caatgaactg gcctcgctgt acgcccagtg cgccgcagca
     3181 ctacgtcacc ccgacgcgct accgattgcc gcaatcgctt gccacggaca gaccatccgt
     3241 catcgacccg actgcggcta tacgctgcaa ctcgtcaacg ccgcgctgct ggccgaactc
     3301 agcagaaccc gtgtcatcgc cgattttcgc agccgcgaca tcgcagcagg cgggcagggc
     3361 gcgcctctgg ttcccgcgtt tcacgccgca gccttccacg atcccgcgaa cgaccgcgcc
     3421 gtggtcaaca ttggcggtat cgccaacctg acccacatcg cggcagatgg aggcatcggc
     3481 gggttcgact gcggcccggg caacatgttg ctggatgcgt ggtgcgcacg ccacaccggc
     3541 caatcttatg accgaggcgg cgtctggggc gccagcgggc aagtattgcc cgaactgctc
     3601 gcccgcttgc ttgcgcatcc gtttttcacc gccgtcccac ccaagagtgc cgggcgggaa
     3661 caattcaacc tggactggct cgaccacctg cgcaccggcc atgaacgccc acaggatgta
     3721 caagccacat tgacgcaact gactgcgctc acgatcgcgc acgcactgcg ggaccactgc
     3781 ccggcaaccc cccgggaagt gtacgtctgc ggcggaggtg cgcacaacga tgcgctgatc
     3841 ggacaattgc ggaaagaact gcctgccgta cagatcgcac tcactgacac gctcggctac
     3901 cccgccgact ggctggaagc acatgccttt gcctggctag gctggcaaac cctgctcggc
     3961 cagcccggca accgtaccga agtcaccggg gcacgccacc cgtgcaccct gggcgcaatc
     4021 taccctccgt gacgcggaaa tgaaacgacc cggactggcc gggtcgttga attcctgagg
     4081 taacgccgac agaagtcggc ggccacactt atgcggaaaa agacgaaccg caaccacagg
     4141 tcgacgtggc attcgggttc ttgatcacga actgggcgcc ttccaggcct tcctgatagt
     4201 cgatctcggc acccaccaga tactggtagc tcatggggtc gatgagcagc gtcacgccac
     4261 ctttgaccat gctggtgtca tcttcgttga ccacttcatc aaaggtaaac ccatactgga
     4321 aaccggaaca gccgccgccg gtcacgaaca cgcgcagctt gagatcaggg ttgccttcct
     4381 cggtgatcaa ctgcgccacc ttggcggcgg cactgtcggt aaacatcagc ggagcgggca
     4441 tttcggttac tgcattcatg gttctgctcc tgaaaaggtt agagaacatg cattatcatc
     4501 gcatgaaagt cttgcgtcaa tagtcagacc ggcagctcat ggcaacaggc cgggcgcttc
     4561 cagcgccagg gtttcagcgt aaccgaacat gagattcatg ttctgtactg cctgccccgc
     4621 cgcccccttg accagattat cgatcaccgc cagcacgacc acggtttcgc cgtcctgcgg
     4681 gcggtgccag gaaatgcgac acgtgttggc accacgaacg ctgcgggttt ccgggtggga
     4741 tccaggcggc atgacatcca cgaacggttc tccgacatag cgctgttcga acagtgcctg
     4801 cacgtccacg gatcggtcca cccgcatgta cagcgtcgca tgcatgccac gaatcagcgg
     4861 tgtcaggtgc ggcacaaacg tcagatcaac cggtttgccg acaatcctgc ccaacccctg
     4921 ggcgatttcg ggaaggtgac gatgcccgct ggcaccgtag gccttgaaat tgtcgctggc
     4981 ttcggcgaac agataaggca attccgtctt gcgaccggcg ccgctgacgc cagacttgca
     5041 gtccgccacc agcatgccgg tatcagccac accggcttcg agcaacggca ggaaaccgag
     5101 ttgcacgacg gtcggatagc agccgggatt ggccaccagc cgcgcactgc ggatctgctc
     5161 gcggttgact tcggacaagc catagacggc ttcggccacc agttccggac aggcatgttc
     5221 catgccatac caatgctgcc agaccgcaag atcgcggata cgaaaatccg ccgcaaggtc
     5281 gatcacgcgc acgccagccg caagcagctc acgtgcctgc tgcatggcga tgccgttggg
     5341 ggtcgcaaaa aacaccacgt cgcagcgatc gagccccgcc tgctgcggat cggaaaacgc
     5401 cagatcaata tgtccgcgca ggctgggaaa catctccgcc accggcatgc cggcttcctt
     5461 gcgcgaagtg atggccgcca gttcaacctg cgggtgacgc gccagcaggc gcagcagttc
     5521 caccccggta taacccgttc cgccaacgat tccggccctg atcatgatgc ctgctccaga
     5581 tatgcaatac gcggcacata tgatacacga gaatcgcgtc aggtcacggc ggacacactg
     5641 gcaccacgcc ttcgctggct acaccggcag tgcgtgacct tttttctcga ccagtcgcag
     5701 cattgcatcg acgacatcgg catcaaacca gataccgcga tggctgatga tttcctgcac
     5761 ggcagcatca atgcccagcg ccggccggta gggtcgatgt gacgccatgg attccagcac
     5821 gtccgccacc gcgaggatgc gcgcttccgg caggatctgt tctcctttca atccatgcgg
     5881 atagccacta ccatcgatgc gctcatggtg ttcgcgaatg atttctgcga ccggcaaatc
     5941 gaacttgatg tccatgagaa tctcataccc tttttcggca tggattttca ccatctcgaa
     6001 ttccagtggc gtgaggcggg tcggtttcga aagaatttcg accggcacgg caatcttgcc
     6061 gatgtcatgc accagcccga ccagttccag atgatgacag cggtcctccg gccagcccat
     6121 ctcgcgcgca atggcagcag caatcaatcc gacccgctgt tcatgccctg cagtgtacgg
     6181 atcgcgcatc tctaccatgg tcgcgacggc gtgcagggtt ttttccatgg attgttcgag
     6241 ctgactcacg taagctgcga ttttcgcttc agcacgtttc cgttcggtaa tgtcctgggc
     6301 gaacacgctg atggcagtcc tgccattcca cacgcccaga ctcgcatgaa tcgccaggtc
     6361 gacgacggta ttgtcccgcg catgaaacgg caacgtaaac gactggctgt gttcccccgc
     6421 gagcagacgc gcacgggcat ccagcaccat gcgccttgcc tgcgcatcat caccgaagaa
     6481 ctccagtgaa tcccgcccca gcaaatcttc gcatttccat cccaggatct cgcacaacct
     6541 cggattgaca tacacgatac ggctgtcct
//
LOCUS       DAIANL010000010        11185 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_212745, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000010 DAIANL010000000
VERSION     DAIANL010000010.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11185)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11185
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212745"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2055 a         3281 c         3558 g         2291 t
ORIGIN      
        1 acgccgatct cgtggctggc cagcttgccg agaccctgcc tgccgagaaa ctgcttctca
       61 tgaccaatac ttcaggagtg ctcgaccggc agggtcagtt gctcaccggc ctgactgcac
      121 cgcgcgtgca cgacctgatc gtcgatggca cgatctacgg cggcatgttg ccgaaagtgc
      181 agtgtgcgct cgaatcggtg cgcaatggcg tgcagacggc gcatatcatc gatggtcgcg
      241 tcaagcatgc gctgttgctt gaagtgctga cccgggatgg tgtaggcacg ctgatccgcc
      301 gtaactgaga tcgcgcggcg cccgttggca ggatttgatc gtggcgcccg tcaggtccga
      361 cgggtggcga atcagtcgat cagcagatcg ggcggggcgt cttctgccag gcggtagtgt
      421 gcggcaagac gcatgtagtg ctgcgccgag tagtacaggt aagcgatctc gtcgccagtc
      481 aggcggcgca ccagtttggc tggacggccc agatacaggt gcccgctgtt cagcaccttg
      541 ccttccggca ccaggctccc cgcccccaac agcacctcgg gttcgatgaa ggctttgtcc
      601 atgacgatgc tgcccatgcc gatcatgcag gcatgtccga tctggcagcc gtgcaggatg
      661 cagccgtggc caacagtcac gtcgttggcg attgacagcg gtgcaccgcc cggatcggcg
      721 aggctgggat gggagacgtg cagcacgctg ttatcctgaa tgttgctgcg gtcgccgatg
      781 cggatgtggt gcacatcccc acgaatcacc gcgccacacc agatcgaact gtcgcggccg
      841 atgtggacat taccaatgac ctgtgcgctg gcatgcaccc aggcattttc ggcaagctgc
      901 ggaatgtgct cgagaaaagg ctgcaggcgc gaacgagaga ttgccatatt tatggtgctc
      961 atcgtctgac aaaagcggta ttatatgcga attgtacgca attgttaggg aagtcgcttc
     1021 tcccggaaca ggaatttcgc ttcatgagca accctttact ggatttttcc gcgctgcctc
     1081 gtttcgatgc aatggatgca agcttcatcg gccctgccgt ggatcagttg ctggcagaaa
     1141 acgctgcgct ggtcgaacag ctgtgtgcag accccactcc accggactgg gcacatttcg
     1201 tcacccccct cgaagacgcc aacgaacggc tggcgcgtgc ctggggcgtg gtttcgcacc
     1261 tgaacgcggt gatgaacagc ccgcaatggc gtgaagccta caaccagaat ctgcccagga
     1321 tcagcgagta ttatactgcg ctggcgcagc atccggggct gtatcgggcg ttttccgcca
     1381 ttcgcaccgc cgacggtttc ggggcgctcg atgccgtgcg gcagaaagtc atcgacaacg
     1441 agctgcgcga cttccggttg ggcggcgctg acctgtccgc atcagacaag gagcagtttc
     1501 gtgtgatcca ggctgaacta gccctgctcg gcgccagttt cgaggaacac ctgcttgatg
     1561 ccaccaatga tttcgccctg ttgatcgaag acgaacaaca actggctggc ctgccgcagg
     1621 acgtgatgga agccgcgcgc gaggcagcgc agcgggcggg atcgccgggc tggaaattca
     1681 ccctgcacgc accgtcctac attcccttca tgcaatatgc cgagtcgcgc gagctgcgcg
     1741 aacggctgta tcacgctcac gtgacgcggg cttcgtcatt cggacctgcg caatgggaca
     1801 attccgcgct gatggaccgc attctcgaac tgcgtcaaca ggcggcgaag ctgctcgggt
     1861 tcgccagcta cgccgaactg tcgctcgaaa caaagatggc cgaaaccccg gcgcagatcg
     1921 aagcgtttct gctcgacctg gcgcagcgtg cacgcccgtt tgccgagcgc gaccgggacg
     1981 agttgctggc gttcgcgcgc agcgaactgg gcatcgagga cgtccagtcg tgggacctgg
     2041 cctgggtgag cgaacggctg cgtcaggcgc gttacgattt ttccgaacag gaagtgcggc
     2101 agtattttcc agaacagcag gtgctggaag gattgtttcg cctggtccgc accctgtacg
     2161 ggctcgatat ccgtccggcg caaaccagcg tctggcatac cgatgtgcgt ttctacgaac
     2221 tcgtcgatgc tgcgggtgcc ctggtcggtc agttctatct cgacctgtat gcgcgtgaac
     2281 acaagcgcgg cggggcgtgg atggacgatg ccattacgcg ccggcgcaag ggtgatgcca
     2341 tccagacgcc ggtggcctgg ctgacatgca atttctctcc ccccgcgggc ggaaaaccgg
     2401 cattgtttac tcacgacgag gtgctgacgc tgttccatga attcggtcat ggattgcatc
     2461 atctgctcac ccgggtggaa gagctggccg tgtcggggat ccatggcgtg gaatgggatg
     2521 ccgtcgaatt gccatcgcag ttcatggaga atttctgctg ggaatgggac gtgctgcagc
     2581 acatgagccg gcatgtcgac agtggcgctg cactgccggt cgaactgttc gatcgcatgc
     2641 tggcggcgcg caatttccag agcggcatgc acaccctgcg tcaggtggaa ttcgcgctgt
     2701 ttgatctgcg gctgcatgac ggcgcgcggc ccgctggcga atcgatcctg gataccctgg
     2761 cgaaagtacg caaagaggtg gcagtctggt ttccgccaca ttacaatcgt ttcgccaaca
     2821 gttttgcgca catttttgcg gggggttacg ccgcgggtta ttatagttac aagtgggctg
     2881 aggtgttgtc ggctgacgtg tattcgctgt tcgaggaaca gggcgtgctg tcatccacga
     2941 tcggtcagcg gttctggaac gaaattcttg ctgtgggtgg cgcgcgaccg gcgaaagagt
     3001 cttttgtcgc gtttcgtggt cgggagcctg acgtacaggc attgttgcgg catcatggca
     3061 tggtctgacg gatccaggcc atcgtagacg gaggacgggc atgcggaaag gtatgggaat
     3121 gctgatggcg tgtgtctttg ccacgccggc gattgctgcc gatgtgtacc gctgggtcga
     3181 ccagaacggc gtggtgaact acagtgacca gctgccgatc ggcatcgcca ggaatttcca
     3241 gcagaaacag atctacgcca acgtgatcga cgcccagccg tcgtactcga tggtgaccgc
     3301 ctctcacaag catccggtgg tgctgttcgg cggcgattgc gggccgttgt gtggcaatgc
     3361 ccgtgcgttg ctggaaaaac gcggcatacc ctacacgctc aaagacccgc agaaaagtcg
     3421 tcaggatgcg gttgaactca accggttgac cggtggcatg aacctgccgg tattgcgcat
     3481 cggggatcag atcttcaagg gattcgaagc ggaaaaatgg aatgaagcgc tggatcatgc
     3541 cgattacccg cgcacgccga ttccgcatga cgggcttcac gccgcagcac aacacgatgc
     3601 agcagcaccc gcatcagcgt catccgcccc ggatgtgccg ccgcctgcgg cgaatgcggc
     3661 tgccccgtct gcttcctctg gtcgctgaat gcgcctcgcc agctggaacg tcaattcgct
     3721 caaggtcagg ctgccgcatc tgctcgactg gctggagttg cagcgtccgg acgtggtctg
     3781 tcttcaggaa accaaactcg aggacagcaa ctttccgttc gatgccttgc agtctgccgg
     3841 ctatcacgtc gcctgtgccg gacagaaaac ctacaatggc gtggcgatat tgtcgcgtga
     3901 gccgctgacc gatgtcgtgc gcggcattcc cgggtggcag gatgagcagc agcgggtgct
     3961 gacggcctgc attggtgcgg tgcgggtggt gtgtgtctat gtgcccaatg gccagagcgt
     4021 ggaatccgac aagtatcgct acaagctgga atggctggct gcactgaaaa cctatctgca
     4081 ggacgaactg cggcgtcatg cgcagctggc cgtgctgggt gactacaata tcgccccgac
     4141 cggcagcgat gtctacgacc cacaggtgtg gaaagatgtg gtgctggttt ccgcgccgga
     4201 gcgcgaggcc ttttctgccc tgctcggtct cgggctggtc gacagtttca gtttgttcga
     4261 gcatgccgaa ccggtattta cctggtggga ttatcgcatg ggtgcattcc gtcgcaacat
     4321 ggggctgcgc atcgatcaca tcctgctgtc gcaggcactg gccggcgact gtagcgactg
     4381 gcgtgtcgat cttgaaccgc ggcggcgcga gcgcccatcc gaccatgccc cggtgctggt
     4441 ggattgcaat cccgatatgc agtctgccgc ttcagcggcc ggctgaattt gcggcgcagc
     4501 cgtcagctgt caagaatttc ctgcgcccga cgcaaaaaat cgctggccag tttgctgtcg
     4561 actgcgagct tgatgaattt ctggcaagcg atcaacgctt cgcccagggc gtcgcgcgtg
     4621 tcctgggcct cgttgatttt cttgatgatg cgctcggcgt gcgggcccag caggctgtgc
     4681 gccagttcca ccagccgtgc cttctggacc aggccgctgt tgaccgggtg ctggtgcgca
     4741 tgctgcattt cgaccaggtc gcaggtgacc agccccagat tgcacaattc ctgcagcacc
     4801 gcttccaggt tttcaaccca gaccacccgc gcggccagat cggccaggga ggtcttgcca
     4861 tccaccgcga tgagcgcccg gcgttgtgcc gtgctcagtt cgcggctatg gcttgcgacc
     4921 atttggtcgc ctttggctgt gcgaaacgga atctgttcgg gtttcatgac gccccccagg
     4981 agaaagccgc gaatctactc gatcagttca cgaatgatgt tgtagatcat gcggatggta
     5041 acggacacgt aaaccgcacc cagcaagaaa atgatcgcta ccagcaggcg caggatgttt
     5101 tgcggcaact ggtcgaaaaa cggcgtcgcg aggaagccga tggtgaacag cgcgaccagc
     5161 atcgacagaa agttccagtg tttgccgaca gtgcccccgg gtatgctgga tttgagcgaa
     5221 agcaccaggt acaggcaata gaacatgaaa atgatggcgg caaagtcgag cactgtcgtt
     5281 atgacgttga aatctccgaa atccatgggt tgtctctcca gtagtattgt ttgttgtgta
     5341 tttgcggtgt tgaatggtac ataaaaccgc catattcttg tcattatgct ttcattataa
     5401 tacaaggttg accgtgtggc gtggaatccg gggctgcgcg agcggacggg ttttaagatg
     5461 ggttaacgtt ttgatttgta tattaaatca ggcagagatg ccgttagttc agacggatgt
     5521 gtatcaccgc gccgatgaca aaacttgcgt agcctgcgcc gccgccgatc agcagcatgc
     5581 gcagtccgcc gcggagggca ccgcggccgg taaacaggct cagcgtcgca ccgatgacga
     5641 acagggcgca ggcactcacg ggcgccacgc gcagcgccag tgaaccaccg ccgaagaata
     5701 gcaacgccag caatggaatt cctgcgccca ggcagaaggc aagaaacgac gatagcgccg
     5761 ctgccagtgg tgagccgagg tcgtcaggat tgaggccgag ttcctcgcgt gccagtgttt
     5821 ccagtgcgcg ttccgggtgg ctgaccagcg tgtgcgcgac atggcgtgct tctgcggcat
     5881 ccatgccgcg ggcgtggtag atcaatgcca gttcctcagc ctcttcctgc gggtagagtc
     5941 tgagttcctc gcgttcgagg ccgatctggt actggtacat ttcgcgttgg gagcgtaccg
     6001 aaacatattc gcccgccgcc atggaaaatg cgccggcgag cagacccgcc agacctgcca
     6061 gcagcaccgt gctgatcgag tccgcggtgg cgccggccat gccgaggatc agactggtat
     6121 ttgacaccag gccgtcgttg accccgaata ccatggcgcg caggttggcc tggccaccgg
     6181 ggtgatgacg gcgaccgatg tcggccgcat cggcaggaag cggtgggtga gccggtggca
     6241 gaccaaacac cgacatgccg cgaattttca gggcggacag taccggtcgc atggcccgta
     6301 cgccaaacca gcgcagcagc caggcgacca ggcggctgcg caggtcgggg cggaatactg
     6361 gaactgctgc atgatcctgt gccagcaggg tatgttgcca gatgccggcc tggcgttcgg
     6421 cactggtggc caggctggcg aacagttcgg cctcgcggcc cgaactggcg gcggccatct
     6481 ggcggtacag ccatgccgaa cgcatttctt cttgccagcc ggaaagtgga tcgggcgtgt
     6541 gcgacatgcc tgttccctgc aatcaatgga tcgtggaatg gaatcgccgg tattgtatgg
     6601 cttctgccac gtgttcgcgg ccagtgtgtc cgctatcctg caggtccgca atggtacgcg
     6661 ccaaacgtaa aatacggtgc cagccgcggg ccgaaagctg cagacgctgc atggcctgtt
     6721 gcagcagcgc gaacgcttct ggcgtggcgg cacagtgccg ctcggtctcg ctggcgttta
     6781 gtgctgcatt ggccttgccc tggcgtgaca gttgccgttc gcgtgcctgg cgtacccgtt
     6841 cgcgtacgga gctgctcggc tcttcgcatg cgttgccaac cagtgcggta ccgggcaatg
     6901 cgggtacttc gatctgcagg tcgatacgat cgagcagcgg gccggacagc ttgttccggt
     6961 aacgtgcaat ctgttccgcc gtacaccggc aggtcgcatc gggattgccc aggtgaccgc
     7021 acgggcaggg attcatggcg gccaccagct ggaagcgggc gggatattcg acctggcgcg
     7081 cggcgcgcga gaggctgatc tgtccggttt cgagcggttc gcgcagcgct tcgagcaccg
     7141 tgcgcgaaaa ttccggcagt tcgtcaagga acaggatgcc gtggtgcgcg agcgagattt
     7201 cgcctggtcg cgggtgggtg ccgccgccga ccaatgctgc cgtcgatgcg gtatggtgcg
     7261 gtgcgcgaaa cgggcgtcgg cgccaatgtt cgctgtggaa atcgcccttg agcgacagca
     7321 gtgccgccga ttccatcgct tctgcttcgc tcatctgcgg caggatgccc ggcagccggc
     7381 tggcgagcat cgattttccg ctgcccggcg gcccgctcat caacagcgaa tggccgcctg
     7441 cagcagcgat ttcgagcgcc cgtttggcac ctgcctgtcc acgcacctcg ttgagatcgg
     7501 ggccgtcggc ggccgacatt gtgtcggtcg gtgaaatatg tggctggatg agtgagtgtc
     7561 gcgacaggtg ggcgcagact tccagcagcg aaccggcggg aaagatgcgt gcttcgcgga
     7621 ccagcgcggc ctcggcggca ttctcgcgtg gcaggaagaa gtcccggccg ctgtcagcga
     7681 cacctgcagc catcgccagc gcgccgcgca ccgggcgcag tgccccgctc agcgcaagtt
     7741 cgccggcaaa ttccgcattc tcgacggcgt ttggaggaat ctgtcctgct gcggcgagga
     7801 tgcccagggc gatgggcagg tcgtagcgcc ctgattcctt gggcagatcg gccggggcaa
     7861 ggttgacggt aatgcgtcgc gcaggaaatt caaaacgcga attgagtatg gcagcgcgta
     7921 cgcggtcgcg cgattccctg acttccgctt cgggcaatcc aacgatggtg aacgccggca
     7981 ggccattggc cagatgcgct tccaccatga cttccggcgc gcgcatgccg gccagcgcgc
     8041 ggctgcgcag aaccgcgagc gacatggatt tattcgctgg cgggagcggc cggggtcagc
     8101 gcgtgttcca gttcggcgac gcgggcttcc agcgtggtca gttgctcgcg tgtgcgcagc
     8161 agcacctgct gctgcacgtc gaattcctcc cgcgtgacca gatcgaggtt tttcagcagg
     8221 ttggcaagca gggcgcgcag attgttttcc atgtccttga gcgggctggt ttgcaaggtc
     8281 tggctgattt tctcgccgat ttcatcgatc agattctggt tgatcatcat ggcagtctct
     8341 gggtaagggt gacggatgta ttttactcgc ctcgtgacgc atcgtgtctt aatttgtgat
     8401 ctggcacccc tgcacgtcca tggcgtgacc gccggaacag cgggtcaggg cccgtgcgtc
     8461 acggttgtcg cactggagtt gcgcgaaccg gttgccattg acagtggcgg gaacgcgcat
     8521 gccgcagccg ctcaataggc aacatcggtt cgcatcgccc agatatgcgc agcggccagg
     8581 gctcgccacg gcgagaactg cgccagccag tcctggaccg cctgtgcgct gatcctgtcc
     8641 ggtgcgtgca acagcgtctg cacgccacgc tgtactgctg catcgccatg cagcgagcca
     8701 tccagccagc cgaagcctct caacaaggtg taattcaccg tccacggccc gatgcccttg
     8761 atcgccagca gttgcgcgcg catttcatcg attggcaggg tgctggccca ggtgtcgagc
     8821 ggcaaccggt cttccatgac ctgtgccgac agcgtcagga gtgtccgggt tttcgtcgcc
     8881 gaaaatccgg cctgacgcaa ggtggcttcg ctcaatccgg cgatgtgtcg tgctcccggg
     8941 taacagagca gaccgctgga gtgacgtata ttcgtggcct caatcagttt gcgtcgcaac
     9001 gctactgctg cgcccagact gatctgctgg ccggcgacag cccaggtcag ggcctcgaac
     9061 ggcgttgccg tcgctggcac gcgcaatccg gggtgatgcg cgatcaaaag accaagttgc
     9121 ggatggtcgc gatggcgcgt ttcaaatgcg tcgatatcct gtgtcaaccc gagcatgcgt
     9181 cggaccatgg actccagggc agtcggaccg atgtccggca gccggccatc gacggacagc
     9241 ctgacttccg cctgtcccgg gccgaagatc acggtcaggc aagcgggcgc atgttgccac
     9301 atgattccct tgtgcagcgc gtcatcggtc acccgctcgg cgatttcctg ccggtcacgc
     9361 cgatgaaagg aaagtgcatc cccggggcgg aacgtggctg gcagaggcag gatcaaccgg
     9421 agctgggtgg tcatggtccg gactcccggt caagcaacgc ctgtttgcgc gcgacgcccc
     9481 agcggtatcc ggacagcccg ccatcgttgc gaaccacccg gtggcagggg atggcaacgg
     9541 ccagtgggtt ggcggcgcag gcctgcgcca cggcacgcac tgccctgggg gcaccgatgc
     9601 gccgagcgac ttccgtatag cttagcgtcg tacccgcagg gatggcgctc aatgcttgcc
     9661 agacccgctg ctggaatgcg gtgccgcgta tatccagagg caaatcgaga ccgcattgcg
     9721 gcatgtccac gaagccgacc acctgggcta cctgttgctc gaatccggcg tccccaccga
     9781 ccagttctgc cctggggaaa cggtcctgga gatcgcgcac cagcgcatcc gggtcatcac
     9841 ccaggagaat ggcgcagatg ccgcgcgcgc tggctgccac cagaatcgat cccagcgtgc
     9901 attcgcctac cgcaaagcgc atgaccacat tcgcgccacc cgcacggaaa tgctttgggg
     9961 tcatgcccag caactgttgc gacgcttcgt aaaatcgtcc actggagttg aaaccggctt
    10021 catagattgc atccgtcacc cggcgactgc gtatcagttc atcgcgtaat ttttgggcgc
    10081 gatgagcggc tgcatatgcc ctgggggtga gccctgtcac cgccttgaat atccggtgca
    10141 gatgaaacgg gctcaactgc gcgctgtgtg ccagtgtgcg cagatccggg gctgtcgtgg
    10201 cgttctcgat ctgtctgcac agctgttcga ccagtgctgc atgccgctcc cggagcgggg
    10261 gctgatccgg tctgcagcgt ttgcacgggc gaaagccggc ttgcgctgcc gccgctgccg
    10321 tatcgaagaa ccggacattt tccgggttgg gcgttctcgc gccgcatgac gggcggcagt
    10381 agacccctgt cgtcctgacc gaataacaga actggccatc catccgggga tcgcgtgtca
    10441 cgatggccgc ccatcgggga tcgcgaagta cggactcggc agatttttcg ctggatgttg
    10501 acatggttga tcattcccgt gtgcttgatg gtacacattc tagccttcgg aaagagcaca
    10561 taggctccgg cgtttgcttt tgaatccatc gggtcccggg cgatccgacc tgatcgggcg
    10621 cacgcatgat gatgctactg ccattttttt acaaagtcct gatgcgttcc tggctaacat
    10681 tcatccggat gagcagattt tggtgcatgc cctgccagcc aatggcatga cgcacctgtt
    10741 tggtgcgcat gacccggggg gtgaaaatat ttcgtgcatt ggaatggtgc gcatgattcg
    10801 tcaacgtcag gcgccatcat cataagcaac tgttttttaa tttgtgtttt ttctggcacg
    10861 gtttctgcta cttgttgcag cgcaatgttg cgcttatttt taccgggagt tacttatgcg
    10921 aatgatgaat aaaacggtgc tggcactggc cctgggcgcc ctgtcccttc ctgcgctggc
    10981 ggatgatacg gcagccgcgc ctgctgcgcc tgcggctccc agtctcggct cggtgatcgc
    11041 cgcgactccg gggctgagta ttaccggtta tgtcgccggt acctatgact atttcagtac
    11101 cgacacgccg ctgatgcgcc agttcgatac cacgcagaat ggcttcaccg ctaaccaggc
    11161 agcgttgacc gtcgcctatc tgccc
//
LOCUS       DAIANL010000011         6101 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_214494, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000011 DAIANL010000000
VERSION     DAIANL010000011.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214494"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1282 a         2018 c         1763 g         1038 t
ORIGIN      
        1 ttcgtcgacc ggcacacctg acacccccgg cgccttgcgc gtcatgctgc tcggcagtgg
       61 cgaactcggc aaggaagtca tcatcgaact gcagcgactg ggcgtggaaa ccattgccgt
      121 cgaccgttat gccggtgctc ccgcgcagca ggtcgcgcac cacgcccgcg tcatcgacat
      181 gtccgatgcc cgtgcgctgc gtgccctgat cgaacaggat cgcccgcatc tggtggtacc
      241 ggaaatcgag gccatcgcca ccgacgaact gctcgccatc gaacaacagg gactgtgcac
      301 cgtaattccc actgcacggg ccgcccaact gaccatgaac cgtgcaggaa tccgccggct
      361 ggcagccgaa acactcggtc tgccgacatc accgtaccgt ttcgccgaca gcttgcacgc
      421 cctgaccgaa gccatcgaac ggcacatcgg cctgccctgc atcgtcaaac cggtcatgtc
      481 ttcctcgggc aagggccagt cgcgcatcga gcgcgccgaa gacattgcgg cggcctggga
      541 atacgcagcc agtgctgcgc gtgtcgatcg cggcgaagtc atcgtcgagg gggtgatcca
      601 gttcgatttc gaaatcactc tgcttaccgt gcgtgcccgt gacgccgacg gacgcattga
      661 aacccgcttt tgcgaaccgg ttggccaccg ccaggtcgat ggcgactatg tcgaatcctg
      721 gcaaccgcag ccgatgagcg cgcgtgctct ggatcgcgcc cgcgacatgg cgcgcacggt
      781 gaccgacaac ctcggcggtc aagggctgtt tggcgtggaa ctgttcgttc gcggtgacga
      841 tgtatggttt tccgaagtct cgccccgacc acacgatacc ggtctggtca ccctgggcag
      901 ccagtaccag agcgagttcg aactgcatgc gcgagccatt ctcggtctgc ccgtggacac
      961 gacactgcgc aaccccgctg catcagccgt cgtctacagc atggtgcgca ccgatgcacc
     1021 acgctaccgc ggaatcgcgc aggcactggc ggtgcccggc agcatgttgc ggctgtttgg
     1081 caagccacag gcctggccgc gacgtcgcat gggagtggcc atcgcccgcg gcgagaacat
     1141 cgccgaagca cgcgaacgtg cccggatggc cgcaagctgc attgaagtat acgccggtgc
     1201 ggacgaggcc tgagtggccc gcgcgtgttg acaactgcaa cggcccggct aacccgttac
     1261 accgcaacgg gttttttaac ttacccccac aatctgtgga taactttgtg tactaaatgt
     1321 tcaaccgacc ctcaaccagg ccaagcgcaa ggattttttc ttgcagccta aattttgcac
     1381 tggcttcaaa tcaataaaaa acatagagtt atattttatc tcccctgtca agattccgat
     1441 gaaaaaaatc accctcatgc tgtggaaaaa ttgatcccgc caaccgccat gatggacaat
     1501 gacctggggc cacgcatgtg gagcgtgagt gccttcaacc ggctgatcca gcacaccctg
     1561 tccagcgaaa tccccctcag ctggatcagc ggcgaaattt ccggatttac ccgtgcagcg
     1621 tcgggccact ggtatttttc actcaaggat gcaagcgccc aggtgcgttg cgtcatgttc
     1681 cgcacacgca accagtttgt cgactggcag ccggaaaacg gcgtccacgt cgaagtgcgg
     1741 gccacggcgg gtctgtttga agcccgcggc gaattccagt tacaggtcga cacgctgcgg
     1801 cgtgccggca tcggcgtgct ggcagaacgc ttcgaacagc tcaggcgcag tctggccgcc
     1861 aaggactgtt tgatgccgca cgcaaacgtc cgctgcccgc cctgcccgca cggatcggcg
     1921 taatcacctc cgcacacggc gccgcagtac atgacatcct caccaccctg cgccgcatgg
     1981 cacccatggt cggcatcatt ctctatccct gccaggtgca gggcgcgaaa gctgccgcag
     2041 acattgctca cgccgtgcgg ctggccggac agcgcggcga atgcgatacg ctgatcgtca
     2101 gccgcggcgg cggcagcatg gaagacctgt ggccattcaa cgaagaggcc gtcgcacgcg
     2161 ccatcgcggc cagcccgata ccggtggtga ccggcatcgg acacgaaacc gattttacca
     2221 ttgccgattt tgtcgccgac ctgcgcgcgc cgacccccac cgctgcggcc agcgtcgcct
     2281 gcacccaccg cgaacagttg cagatcgcgc tcgatggcct gcagacgcgg ttagatggca
     2341 tcatgcatac tcgatttacg cactggcagc agcaactgcg ctggtttgca gcgcgtctcg
     2401 tacatccagg cgatacactg cgcctgcgcc agcagacgct gcagcaattg gccggaagca
     2461 tgcggctgca ggcggcgcac ctgcaacagc ggcaacgctg gcgcctggag acgcttggcc
     2521 agcggctggt gcgcgcccgt ccggagatgc cgcaggccgc gctgcaacgc accgcgatgc
     2581 gactggttca ggccatgcgc aggcagatga tgcatcgccg tgacgccacg gaacacatgg
     2641 cggaacaact cggacacctc aacccgctcg gcgtgctggc acgcggttac gcgctggtga
     2701 ccgatgccga aggtgtcatc gtacgcgacg ccggcaacct gcatcctggc agcgccattt
     2761 acctgcgtct gggcctcggt acggccgagg ccgtggtcag ccggaccggc agcggtgcgg
     2821 acgaccccaa ccttgaaaat cagactgttt gacgttacaa tgtccggctt taccgaacga
     2881 cggatttcct tcagccggaa cccgccgcga ttttcaatct catcatcagg agagacacca
     2941 tggctgttac aacccagcaa aaagcccaga tcgtcgaaca attccagcac cagcccggcg
     3001 acaccggctc atccgaagtg caaatcgcgc tgctcaccgc acgcatcaat ggcctgtccg
     3061 accacttcaa ggccaacagc aaggatttcc actcgcgtcg tggcctgctg aaaatggtca
     3121 gccgccgccg caagctgctc gattacctca agcgcaccaa cgacgagagc taccgcgccc
     3181 tgatcagcaa gctcggcctg cgcaagtaac ttcgcatcag cctcggccgc acacacatgc
     3241 atccggtatc ccgttgcgtc cattccgggc gcaacggcgt gtcgaacagt taaaggatgg
     3301 tcatgaaaat caccaagtcg tttcaattcg gtcgccatac tgtcaccctg gagactggag
     3361 aaatcgcccg tcaagccagc ggcgctgtac tggtcaatat ggatgacacc gtcgtactgg
     3421 tcaccgtggt aggtcaaaaa acggtcaagg caggacagga cttcttcccg ctcaccgtcg
     3481 actaccagga aaagacctat gccgccggcc gtattcccgg cggttttttc aagcgcgaag
     3541 gcaagccctc cgaaaaggaa accctgactt gccgcctcat cgaccgcccg ctgcgcccac
     3601 tgttcccgga aggcttctac aatgaagtgc agatcattgc tgccgtgctg tcgagcaacc
     3661 cggaaatcga ttccgacatc cctgccctga tcggcgcctc cgccgcgctt gccgtgtcgg
     3721 gcatcccctt tgccgggccg attggcgccg cccgcgtcgg tttcatcaac ggcgaatacg
     3781 tcctcaaccc ggacagcagc gaactggcgc aatccgcact ggacctggtc gtggccggca
     3841 ccgaacaggc cgtactgatg gtcgagtcgg aagcgcgcga attgcccgaa gacatcatgc
     3901 tcggcgccgt cgtctacggt caccagcaga tgcaggtcgc cattgcagca atcaacgaac
     3961 tggccgacga ggtcaacccc gaaccgtggg actgggccgc accccagccc aaccaggcac
     4021 tgaccgaccg ggtggcagaa ctggccgagt ccggcctgcg cgaagcctac ggcctgcgcc
     4081 agaagcaggc acgttcgcag cagatcgacg cggtgcgtcg tcaggtcgtc gacgctctgg
     4141 tcacggcgga aatgggcaca ctggaagtca acgaaatcaa cgacatcttc cacaatctcg
     4201 aagccaggat cgtgcgcaac cgcattctga gcggcgaacc gcgtatcgac ggccgtgaca
     4261 cccgcaccgt acgtccgatc agcattcgta ccggcgtgct gtcgcgtacc catggctcgg
     4321 cactgttcac ccgtggcgaa acccaggcac tggtggtggc caccctgggc accggtcgtg
     4381 acgaacagaa gatcgacgcg ctgcaaggcg agtacagcga ccgcttcatg ctccactaca
     4441 acatgccccc ctatgccacc ggcgaaaccg gacgcgtcgg cacgccaaaa cggcgcgaaa
     4501 tcggccatgg ccgcctggca aagcgcgccc tggcagccgt gctgcccgac cgcgaggcat
     4561 tcggctacac catgcgtgtc gtgtccgaaa tcaccgagtc gaacggctcg tcctcgatgg
     4621 cctcggtgtg cggcggctgc cttgcgctga tggatgccgg cgtgccgctc aaggcccacg
     4681 ttgccggcat tgccatgggc ctgatcaagg acggcaaccg ctttgccgtg ctcaccgaca
     4741 ttctcggcga cgaagaccat ctcggtgaca tggatttcaa ggtggccggc agcgaaaccg
     4801 gcgtcactgc gctgcagatg gacatcaaga tcaccggcat caccaaggag atcatgaagg
     4861 ctgctctgga ccaggcgcgc gccggccgca tgcatatcct cgaactgatg aagcagacaa
     4921 tcggtgcgcc gcgccaggag atgtcggctt atgcgccgcg catcatcacc ctcaagatca
     4981 atccggaaaa gatccgcgat gtgatcggca agggaggcgc cgtcatccgc gcactgaccg
     5041 aagaaaccgg cacgcagatc gacatcgaag aagatggcac cgtcaagatc gcctgcacca
     5101 gcgtagaatc gggcgaggct gcccggcaac gcatcgaggc gatcacggcc gaagtcgaag
     5161 tcggcaagac gtacgaggga acagtcgtca agctgcttga ttttggcgca atcgtcagcg
     5221 tacttccggg caaggatggc ttgctgcaca tttcgcagat cgcacatgag cgggtcaacg
     5281 ccgtgagcga ctacctcaag gaaggccagc acgtcaccgt caaggtgctc gaagccgacg
     5341 aaaaaggccg cctgcgcctg tcgatgaagg ccctgatcga cgctccggcc agatcggaag
     5401 aagccaccag taccacccag cagtaaatgc acgccgccag cccgtcggct gagcggcaaa
     5461 gcaaacggcc attgcggata cgatctgcaa tggccgtttg cttgtgacgc gccggttcgt
     5521 gcagcgcgac aaaccgttca acgcgcggcg agaaagcctt cccaccagcg cttgcggtta
     5581 ttgggcacga cactcccctc gcccagcggc tccggcggca cctggctcat gatccatccc
     5641 ggcatcaccc gcgtcttgct cgacccgcac gaaacaccga ggtggtgcgg gtcataaatc
     5701 accacgaacg tcggcacctg aacatcatca gcataaacga tgccacaata acgttcttca
     5761 tgctccgcat gcagcaacgc atcgatctgc tcgatgaaac gggccacatt ctccgcatca
     5821 gctggctggg tgggcacctc ctgcccgacc gcgtacagat accagccact gccggcattg
     5881 atgcgcggcc agaaagccgc aagctggtcc cacgatagca ggctgtagag cagcccgtcg
     5941 aattttgcgc gaaagggagt catcgggtca tcctctggat gtgcctgcca atgggagcgg
     6001 catgaaagca accggcatgc cgcgctcccc gggcagtaaa cagaaactga tcagcgtgct
     6061 acctgccgct cgcggaactc gtccagcgtc ttgcagtcaa t
//
LOCUS       DAIANL010000012         9019 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2666484, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000012 DAIANL010000000
VERSION     DAIANL010000012.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666484"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1685 a         2552 c         2930 g         1852 t
ORIGIN      
        1 caggttgccc tcggcggcga cggtcaggtg acgctgggct cgatcgtcat caagagtacg
       61 gcgcgaaaaa tccgcaagct gcaccatgac caggtgctgg caggctttgc cggcgcaacc
      121 gccgatgctt tcacgttgtt cgagcgcttc gaggagaagc tcgaaaaata tcaggggcat
      181 ctgttgcgat cggcggtcga actggccaag gactggcgca cggaccgcat gctgcgtcgt
      241 ctggaagcga tgctggcggt ggccgaccgc gagtattcgc ttatcctgag cggaaacggc
      301 gatgtgctgg aaccggaacg gggcgtggtg gccatcggca gcggtggtgc ctacgcccag
      361 gcggcggcgc gtgcgctgct ggataacacc acgctggcgc cggccgaaat cgtctcgcgt
      421 tcgctggcca tcgcgggtga catctgtatc tataccaatc agaaccacat tgtcgaaata
      481 ctggaatagc catggatagc gtaatgccgg tcgtgatgac tccgcaggaa attgttcacg
      541 aactggataa atatatcgtc ggccagcctg cggcgaagcg cgcggtggcc atcgccttgc
      601 gcgaccgctg gcgtcgcacg tgtgtggccg aaccgctgcg ccacgagatc acccccaaga
      661 atattctcat gatcggtccg acgggcgttg gcaagacaga gattgcgcgc cggctggccc
      721 ggctgaccaa tgcaccgttc atcaaggtgg aggcaaccaa gttcaccgag gtcggttatg
      781 tgggccgcga tgtcgattcc atcgtgcgcg acctgatgga gaccgcagtc aagcagcagc
      841 gcgagcagga catggccagg atgcgcatgc gtgccgaaga tgcggcggaa gaacgcatcc
      901 tcgacgtgtt gctgccaccg gggagcgatg aacggccgat ggacggcgag gtgtcgtcta
      961 cgcggcagaa attccgcaaa atgttgcgcg aaggccaact ggacgagcgc gagatcgaga
     1021 tcgaactgtc cgggtcggca ttcagtgcgc agattctcgc accacaaggg atggaagatt
     1081 tcaccagcca gatccaggat atgttccaga atatgtcgcg ccggccgcac aaggcgcgca
     1141 agctcaaggt caaagaggcg atgaaactgc tcgtcgagga agaggcggca caactggtca
     1201 acgaagatga catcaagcag cgcgccctgc tcaacgtgga gcagaatggc attgtgttca
     1261 tcgacgagat cgacaagatc accggacgtg gcggcaacgc cagtggcggt gaagtgtcac
     1321 gtcagggcgt acagcgtgac ctgctgccgt tggttgaggg gactaccgtc aacaccaaat
     1381 atggcatggt gcgcaccgac catgtgttgt tcatcgccag cggcgcattc cacctgtcca
     1441 aaccgtccga tctcattccc gagttgcagg ggcgttttcc catccgtgtg gagctgcaat
     1501 cattgtcggt ggccgatttc gaggccatcc tgacggctac cgatgcttgc ctgacgcggc
     1561 agtatcaggc catgctggct gtggaagggc tcgatgtgca gttcagtgac gagggaattc
     1621 acagcctggc gaccatcgcc ttcgaggtca atgagcgcac ggagaatatc ggtgcgcgcc
     1681 ggctgcatac cgtgatggaa cgcctgctcg aggaagtgtc atacgaggcc cccaatgcgc
     1741 gcgacaagac gattctcgtc gatgccgagt tcgtcagcca gcggctgggc ggactggtgg
     1801 tcagcgagga tctggcgcgc tacgtgctgt agcgctggac gtcacccggc gcaggggcgc
     1861 tgtccagccc atgtcggttc atcagcggcc gcattgcgga cagcaggttg gcgaacagtt
     1921 gcggatgctg tttttcctgc gcatcgagca gctctttcat ggcctgacgc tgggtcggct
     1981 gcgcgaaaca ggccggacag ttttcatgga tgaccggcaa ccgggcggcg cgtgcgaaac
     2041 tggccgtctg ccgctcgcgc gcgtagacca gcggacggat gatgcgcaag tcgccggcgt
     2101 cgttggtgta atgtgcctgc atggtgcgca acttgccacc aaagaacatg gacatcatca
     2161 aggtctctgc gagatcgtcg aggtgctgcg ccagcgccag cacggtgtag ccttgttcgc
     2221 gcgccacgcg gtagaggatg ccgcggcgca tgcgcgagca ccaggcgcag aacgagtctc
     2281 cactcatgtg ggttctggcg gtctcgacga tcggctggcg ctcgaagtgg taaggtacgc
     2341 ccaacgccgc catatagggg atcagcggcg aaggatcgaa atcgggcgat tgcgggtcga
     2401 ccgtgcaggc accgagttcg aaatgcaccg gcgcgcggct tttcaggtcg agcagcacat
     2461 gcagcagagc cagcgaatcc ttgccgcctg acagtccaag cagtacgcgg tcgccaggac
     2521 ggatcatggc aaaatcgcca atggcgcgac cgcaggcgct cagcagcgtg cgcggcggtt
     2581 tgaccggttg tgacggcggc ggcagttcag tgtccgggtt catggaattg catgcgatgc
     2641 agttgtgcgt acacgccctg aagggcaagc agttcgtcgt gacgaccgat ttcaacgatg
     2701 cggccgtgct gcatcaccgc gatgcgatcg gcattttcaa tggttgacag ccggtgcgcg
     2761 atgaccagcg tggtgcgtcc ctgcatgagg gtgtcgagcg cagcctgtac gtgtcgttcg
     2821 gattcgttgt ccagcgccga ggtcgcttcg tcgagaatca acacgggtgc atccttgagc
     2881 agcgcgcgtg cgatggcgat gcgctggcgt tcgcccccgg ataatttgac accgttttcg
     2941 cccaccagcg tgttgagtcc ggccggcatc gcgcgaatga actccagcgc gtgcgccgct
     3001 tcggccgcgc gcaggatgtc ccgctcgtct gcccggattt gctggccgta ggcgatgttg
     3061 gcggcgatgg tgtcgttgaa caggattacg tcctgtgaaa ccagtgcaat gttggcccgc
     3121 agcgagtcca ggcgcacatc ggacaggtcg tggccgtcga gcaggatgtg tccgtcagac
     3181 ggatggtaga aacgcgggat caggttgacc aggctggtct tgccactgcc ggaggcgccg
     3241 accagggcca gggtttctcc cggctggatg tcgagggtga tatggtcgag agcgggtgcg
     3301 gtcttttccg ggtagcacag gctgacgtcg tgaaattcga gccggccgtg tgcccgaccc
     3361 agtcgtactg tcccggtatc ggtttcgggt gcggtatcca tgaggttgaa gaccacctcg
     3421 gcggcagcca gtccgcgctg cagctgttcg ttgatcccgg tcagtcgctt gatcgggccg
     3481 aatatcatca gcatggccac catgtaggac acgaagccgc cgaccgtcag ttcgccattg
     3541 agcgcctggc gggtggcgat atagaccacc agcgccacgc cgattgccgc gaccagttgc
     3601 accaccgggc cattggccga ggctgcgctg accgccttca tgtacagttt ccggtcctgg
     3661 ttgaccgctg cactgaagcg gcgacgttcg tagggctggc cgccgaaaat cttcaccact
     3721 ttgtgcgctc cgaccgactc ttccacgacg tgagcgattg cgcccaggtt gcgctgcgcg
     3781 tcgcggttga tttcgcgcaa gcgccggctg acgctacgga tgacaaaaat cgccagtggt
     3841 gcgatggcga tgatgatgag cgtcagtttc cagttcagcc agatcagcca gcccagcaga
     3901 ccggcaatgg ccagggtgtc gcgcaccagc agcgtgatgg cgccggtggc agactgggtg
     3961 acctgagtga cgttgaagac gacattggcg atcaggttgc cggtcgagct atcgtcgtag
     4021 aaccgtgtcg gcatggcaat cagccgctcg aacatggcgc tgcgcaaatc ctgtaccagt
     4081 tggctgccga tccagctcag gccgtaggtg ctggcgaagt cggccgcacc gcgcacgagg
     4141 aacagtgcga tcatcagcaa ggggatgagc cggatcgact gcatgtcctt gtgcacgaaa
     4201 ctgccatcga gcagcggctt gaagatggcg ggtaccgccg gctctgcagc ggcagtgatc
     4261 gccatggcca gcaatgaccc ggcgaacatg cgccagtacg ggcgcacatg ccggagcagg
     4321 cgcaagtaca gcctcaggct gtgaggttga gcgggattca cgcgccgaac acctgttccc
     4381 tgagtcccca tttggcggcc agtgtttcca cggcattgcg gatttcctcg gtacgggcag
     4441 gatgattgta gaacgtgtgc gccagcttgt tcaggccttc atcattgccg agctggaccg
     4501 acagggtgtg gatgatttcg ccggcttccg aaccgatcac ctcggcaccg accaggtggc
     4561 cgttgtcgat gtcggcgatc aggcggacga acccggtgga ttcatcctgt cccacggcgc
     4621 gcggattggt ctcgaatgcg gcaaagccga ctgccggctc gtaaccggag tcgatggcca
     4681 tgtcttcgct catgccgata cggccaagtt cgagcgcaga gtagaccatt tccggcacgg
     4741 tatcgatttc aagcaggcgc gaacgcggtt tgaggatgtt ggcgatggcc agatcggcat
     4801 cagccagcgc gtggttggcc gtcatcttgt gattggcaac gtcgccgatg gcgaagacgt
     4861 gcgccacgtt ggtctgtcgc caggggtcga ccttgatgaa gccatggcga tcggtctcga
     4921 ctccgatggt gtccagacca agccccgcgg tatgcggttc gcgtccggca ccgagtacca
     4981 gccagtcaac ctgttcctgg cgaccatcgg aaaaatccag acggacatgg tcgtcgtcga
     5041 cgctcaccct ggaggggcgt ggcagacgga taatgtcgta cttgagcagt tcggccttga
     5101 gcagatccag cgctgcgggt gagaaatcgc ttcgcgacag cggcggtcgg tcggccagcc
     5161 acaatacctt acagccgagt tgggtcagga tgtaggcgaa ttcggtgccg atgacgccac
     5221 tgccgaccag ggcgatgcgg cggccgggcg gcggcggtgc gtcgaacagc tcgtcggtgg
     5281 tgagaatgcg tcccggagtg agtgtgtagc cctcggggat gtgcggatgc gagccggtgg
     5341 caatgatgaa gttgcgtgcc tgcagttgcc tgtcgccgac gacgatggtc tgggcatcga
     5401 cgaagcgggc ggtgccgtgc cagctgtgaa tgccggagcg tcgcaggtaa tcggtatagc
     5461 tgctgcggat gtggttcacg gtttcgtgct gatgttgcca ggctgcctgc aggtcaccgt
     5521 gcaatgtgcc ggtaaagccg cggtgggtcg agtgctggct ggccgcaatc aggttggccg
     5581 tgtggtgcca gtcctttttc ggcacgcagc cgcggttgag gcaggtgccg ccccagacgt
     5641 ccttttccac gatgccgacg tccagcccgc gcagtttgcc gagaacggct gcccggtagc
     5701 cgcccgggcc ggaaccgatc accaacaggt cgtaaacgtg catgatgtag tcttcctgaa
     5761 atcgaaattc ggcgaaacgg caaattatac ggcaatcgcc gattgaatgg aggctcgtgc
     5821 ggccgcaacg ggtcagtgtt ttcctgcggt tggacaggtg tggctgcttc gggttgcaac
     5881 cggtaccaaa aaacaacaac ccccgccgta gcgggggttg cgctgtccgc cagcaggcga
     5941 acagagggtg tgtcgggcac agccgatggc aattacatca tgccgtccat gccgcccatg
     6001 ccacccatgc cgccgccggc aggtgccggc ttgtcttccg gcagttcagc caccatggcg
     6061 tcggtggtca gcatcaggct ggctaccgaa gcggcgttct gcagtgcagt acgggtgacc
     6121 ttggtcgggt cgagcacgcc catttccacc atgtcgccat agacgttggt ggcagcgttg
     6181 tagccaaagt tgcctgcgcc ttccatgacc ttgttcacga ctacggaagc ttcttcgccg
     6241 cagttgatca cgatctgacg caggggctcc tcgatggcgc gcagcacgat cttgatgccg
     6301 gcatcctggt catggttatc gccagccagg ccgccgatac ccgacttggc gcgcagcagg
     6361 gcgacaccac cacccgggac gataccttct tccaccgcag cgcgagtagc gtgcagcgcg
     6421 tcttccacgc gggctttctt ttccttcatt tcgacttcgg tggccgcgcc gaccttgatc
     6481 acggccacgc caccggccag cttggccttg cgttcctgca gcttttcctt gtcgtagtcg
     6541 ctggaggctt cgtcgatctg acggttgatc tgggtgatgc gcgccttgat cgcgtcttcc
     6601 ttgcccacgc cgtcgatgat ggtggtgttt tccttaccca cttcgatgcg cttggcctgg
     6661 cccagttcag ccagcgtcac cttgtcgagc gacaggccga cttcttccga aatcactgtg
     6721 ccgccagtca ggatggcgat gtcttccagc atggccttgc ggcggtcgcc gaagccgggt
     6781 gccttgaccg agcaggtctt gaggatgcca cggatgttgt tgaccaccag cgtagccagt
     6841 gcttcaccat cgacatcttc ggcgatgatc agcagcgggc ggccagcctt ggcaacttgc
     6901 tcgagcgcgg gcagcaggtc gcggatgttg gagattttct tgtcatgcag cagcacgaaa
     6961 ggattctcca gggcagcgat ctgcttgtcg gggttgttga tgaagtaggg cgacaggtag
     7021 ccgcggtcga actgcatgcc ctcgaccacg tcgagttcgt tttgcaggct ggtgccgtct
     7081 tcgacggtga tcacgccttc cttgccaacc ttgttcatgg cctcggcgat gatgtcaccg
     7141 atcgaggatt ccgagttggc cgagatcgaa ccgacctggg cgatttccct ggtggtggtg
     7201 cagggcttgg agatcttttg cagttcgccg acgatggcgc ccacggcctt gtccatgccg
     7261 cgcttgaggt ccatcgggtt catgccggcg gccacgaatt tcatgccttc cttcagcatg
     7321 gcctgggcca gtaccgtggc ggtggtggtg ccgtcaccgg cgacgtcgga ggtcttggag
     7381 gagacttcct tgaccatctg cgcgcccatg ttctcgaact tgtccttcag ttcgatttcc
     7441 ttggcgaccg aaacgccgtc cttggtgatg gtgggggcgc cgaatgcgcg gtcgagcacg
     7501 acgttacggc ctttcgggcc gagggtaacc ttgactgcgt ctgcaaggat attgactccg
     7561 atcatcattt tctgacgtgc gacgtcgccg aatttaacat ctttggctgg catgagttgc
     7621 tcctgaaaaa aattcgatca attgggttga gtgcaatggc tgcgaggcga agcgtggatt
     7681 attccacgat gcccatgatg tcttcttcgc gcatgacgag gacttcttcg ccatccacct
     7741 tgacggattg accggcatat ttgccaaaca gcacgcggtc gccgactttc acgtccaggg
     7801 gacgaacttt gccatcttcg ccgatcttgc cattgccgac agccagtact tcgccctgat
     7861 cgggtttttc ggtggcgtta tccgggatga cgatgccgga agcggttttg cgttcttctt
     7921 ccatgcgctt gacgatgaca cggtcgtgca gcggacgaat tttcattgcg atttctcctg
     7981 tgattaaaca aacgttgcgc gcaatcgccg gattgcgtta aacaaaattt gcgcccggac
     8041 gggtgcggtt ttggcactct cgcccgggga gtgccaataa tggggctggg tgggtagttt
     8101 ttcaaggggc cgaggtgatt tttttgtatg gtactttggg gttgacaggg cttgaatcgc
     8161 caccgatccg gacctacatt gttgtcattg gcaaggtctc ggtcgtgaat cagggatgca
     8221 cggccgtgga ctgcagtctt caggaagaca atcaggactg gtcggtgggc ggtttgcgga
     8281 cggccaccga atattctgcg gtatgtgttc cgcatgttca aggagaagat catggcactt
     8341 atcaagtggg aaccgatgcg cgaatttgaa gacttgtttg accggtatac ccgggcgatg
     8401 ggctggcctg ccaatcgcgg ccaggaactg atgaccaagg gtgactggct gccgcgtgtc
     8461 gatatcagtg aaaccgatgg cgagtttgtc atcaaggcgg aaattcccga agtacgcaag
     8521 gaagacgtca aggtgggtgt ggacaatggc attctgacca ttcagggcga acgcaagcag
     8581 gaaaaggatg aaaagaaaga gaaattccac cgtgtcgaac gctattatgg cagtttcgtg
     8641 cgcagtttca ctttgccgga taacgtcgat gaagcgggta ttcgtgccag tttcaaggat
     8701 ggcatgctga acctgacact gcccaagagt gcggtcgcca aacccaagac cattgaaatt
     8761 tcggtagagt gattgaccgg tgtctgccgc agaaacatcg ctgcggacac cggctaccat
     8821 ggcatgagcc ggtgacggac cgtgtttttg tcctgcgcca gcagctgcat gcgcagatcg
     8881 tgcagtacgg ccaggcggcg ccggttggat gccgtgtcgc cgagcagcac gcatgccaca
     8941 tgccctgctg tggctgcagt ggcaatttcg ctgcacggtg gcgcacgctg tccgtctggg
     9001 ctttcctcga cctgcgtcg
//
LOCUS       DAIANL010000013         9952 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2464160, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000013 DAIANL010000000
VERSION     DAIANL010000013.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9952)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9952
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2464160"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1965 a         3249 c         2894 g         1844 t
ORIGIN      
        1 ctgtcggcct ccgtcgtact gctgccacat ctggaagaac cggtgcgccg gatcgccggt
       61 gtgcgcgccg aaattgacgt aacgggtaat ctggaacgga ccgggcggca tgtcgccggg
      121 aaagcgctcg tccggcacat cctgacgctg gccgaaactt ccgtccgcat agggttgcgg
      181 cagtacggca tagcttccgg cacgttgcgg cgtgaccgaa tacttgccgt cctggcgagc
      241 gatgcgctgc agcgcttgcg cataattggg cccgggggtg ccatcggcgc gcacgatgcc
      301 tcgcgacagc agattggcaa tggtctgccc gcctggcggc acataggttg caaacaggtt
      361 gtcgaatgtt cggttctcgc ccaccacgac gatcacatgc cggatcggcg tgctcgtcga
      421 cgcttgtacc gtgctgtctt gcgccagcgc agtaccgaca cacgtaccgg caacgagcat
      481 cagcgacagg ctggttacgg ccggaatatt ctgtgacgtc cgcatctcag gccttgaccg
      541 cctgcagcgc cacttcgccg ctgccttgcc gggcgccgct gcgcccattc gaggcgctgg
      601 acagccacgt cagcgccagc aatttgatct gtgtactgat ctgttcgacc gtcttgctga
      661 agaagttgaa gaacatcagg ctgtagagcg caatgaaaat accaaacccc gtggcgtaca
      721 gcgccgtacc gatgccacgg ctcaccgccg ccggatccga actgcctgct cccgaggaca
      781 ggttgtagaa tgcatcgata atgcccagga tcgtgcccag caaaccgagc aggggcgaca
      841 gcgtgatcac cgtatccagt atccacagcc gcgactgcag catcgactgc ttggcgacgt
      901 agatcgattg cacgaaatgc tcggtctgct cgtgtcccag tctgggggcg ctgacgatct
      961 cgcacagtgc ttgcgcctgc gggctgcggc gcccgctgca ttctgccagg atctcgtggt
     1021 gcagatcggc gtcgtgcaga tgcgatttca tgaattcggc caccgtcttt ccatcgcgca
     1081 acaccgacgc gaagaaaatg aagcgctcca cgatcaccca ggtagccacc agcagaacag
     1141 ccaccatcag atacatgaca gcgtcatgta ttgccatgaa attcattgga attcccttcc
     1201 gttcaaacag gaatggtgcc agactcgcgc ccgacaccat gtcaccagat tgctcaataa
     1261 cgaccgctca acgaaatgaa ggccatgcgc ggcgcacctt gctgcagggt atagtacgaa
     1321 ccggtcggat cggatgcgac aaaaccgttg gtgccgaccg agctgaaata atccttgttg
     1381 aacagattgt agacattcag ctgtgcccgc acatcctgca acgcacccac gtgtttgaac
     1441 tggtatccgg taccgaagtc gaacagcaca aaggccggca ccgacgaatc gttggtgtag
     1501 gtgatgtagc gcttgtccgt atacttgccg gtcagattgg cgaaaaaccc ttgcttctca
     1561 tagccaagtc gcattgcgta catcagcttg ggtgcgtcca ccaccgtttt gcccgaggtg
     1621 ttcaccagcg tcgttccggc atagtaattg gagaggtaac gcgaatcgtt gtaggtcagg
     1681 ctattgagca acgaccagtg cgccacgggt gaccagttga ccgccgcttc cagacccctg
     1741 gcgttcacgt tgccgacgtt ggccagactg ttcggacaac ccacgatgcc ggcgcagtcc
     1801 ggaatgatca gcagccggtc atggaagtcg acattgtaga ggtccagcga ggcctcgaga
     1861 cccggccggt gggtacgcac gccgatatcc agcgtggtgg aggtttccgg tttcaggctc
     1921 gacgctgtgg catcaaacgc cgcctgcgtg gcagaaaacg gtccggccac acccggctgg
     1981 aaggcacgca tgttcttgcc cagcgaggca tatgcttcat tgtgggggtc gatctggtaa
     2041 ttcatgctga tctggggcag cacaccgtca gtggtgatgt tgcccgcggc cagaccgccg
     2101 atctggttga ccgaatcgat gctgacatgc tgcgacttga tgccgtaagc cagcgtcagc
     2161 cggtcatcca tcaacgacag cgtatcctgc aggtacaatt gccgtgtatt ggtcacgaat
     2221 tcctgctgaa attgcgtgta gaacggattg gtgagaaagt aatccgtgct ctgcggaccg
     2281 ttgatggcgt aatagcgtcg cgccagatca tgcacattgt gttcgatcca cacgccgcca
     2341 gtaatgtcat gcataccgag cagccagtcg aacgaagcat tcacgccttc gcgatggatg
     2401 ccgtattcgg tggtacgcac cgagatcggc acagtcggtg acgaggccac gtacggcgtg
     2461 taccagtcgc cttcaccctt gtccatgtga ccgtagacca cggccttcac gcgcaggttg
     2521 tccgccagct tgaactggcc ggttgcgcca gccagctcgt cgttgcgcag accgcggccc
     2581 agatagtaga aggcgtcgag cggatcctgc agattctgca caccaccgct gtaaatgcca
     2641 ttggcggcat tgactgcgcg cgtccagttc ggcgcgtaat tatcctgccc ccagcccaga
     2701 acattgactt cattgaccga ataatccgcg tagtcggttt cgtcgcgcgt ggaggcatca
     2761 taataaaacg acagcttgtt ctcgccaccc agctggtata ccagcttggc gttgaacatt
     2821 tcctgtgtct ggtcgcccca gcctttccat ttgctggtgg cattgttcga ataactgaaa
     2881 taggctttgg ccgcgttgct ggctccgaac gtgccgctgt cccagcgcat gtacacccgg
     2941 ttcagattgg cgctgccgcc ggtatcggcc gccgtgaaat gcgctgtatc gagtggatca
     3001 gccgactgga actggaccgt gccgcccagg ttgctggtcg atgcagtacc cagcgcgccg
     3061 ataccctgcg cgaccgacac caccgacagg ttctccggtg caatcgcccg gccgatgtcc
     3121 agaccgttgt agttgccata gctcatgtcg cccagcggga tgtcatcgag cgtgaagccc
     3181 agctggttct ggttgaagcc gcgaatattg aattcggccg accactcgta attgccgagc
     3241 ggatccgaag actcgtactg cacaccaggc aacacggcca gtgcctgcag cgggttggtg
     3301 cccggagcca gctgctgcag ctgcgcctgg gtcagcgtct gcacttcgcg tgtctgtccc
     3361 tgccccagca ccgatacatc ctgcgtcgtc acggcactgc cctgatcatc cgcatgtgtc
     3421 attgccggca tgcacatgca cgccgccagc accgccgccc gcagtggacg catcctgaaa
     3481 cccgctttca ccattttcat ttccccaaca cacatgaata aaaaagccgg tcaatcaacc
     3541 agcttgaaca ccaccctggt tctgaattcc ttcctgtctt cgcccggcca gaacgtgtct
     3601 gccatggcgc cgaagctcgc cgagcgcacc gcccgcagcg ccgctcgatc aagcagctcg
     3661 taaccggatg aagtcaccac ttccgcggcc agcaaccgcc ccttgcggtc gatgacataa
     3721 cgcagctcga ccacgccctg catatcgcgc tgacgtgcag cagccggata tatcttctgt
     3781 tcttcgattc gctcccgcac ggcgcgcgca aacccggcct cgccagcaaa gtcttctccc
     3841 ggagcgggcg cctcattgcc ggcagcggcc gccggagcgg gcgcgggcgc cgctgccacc
     3901 gggccaggcg tcgacactgg caccgccgcc acagccaccg cagcggctgg agcaggcacg
     3961 cgaaccggca ccgcctgcgg ccgtggatgc ggctgtgggt gcggcatcag gggctgccgc
     4021 ggcggtgacg gctgcggtct gggaggatgg acaaccggtt tcggtgcatc cttcggcagc
     4081 gttaccagcc gcacctgcac cgcaacgggt ggccgcacgt ccggtcgcat caccagtatt
     4141 ccgcgcaccc cggtcagcag catcagcagc agcaatacca cgctgccggc cacccgatcg
     4201 cgataggcaa acatgtcgac gctcatcggc gcttatcgcg gcttgctggc aatcgcaatg
     4261 ctctggatgc cggccgcctt gaccacatcc atgacatcga ttagcgcctg catgtccacg
     4321 cccttgtcgc cgctgaccac aacgtccacg gcaccgcctg cccgcgttgc ctgcgtcagt
     4381 cgcgccgaca gcccgcccag gctgcaggac tgcccgtcca tcgacagttc acccgagcgc
     4441 gcaatcacaa tcaccagatg gcgtgccgga cgaatgtcct cacgctggga agcggctggc
     4501 aattcagtcc tgaccccctt ggccgggatc acattcaggc tgagcagcac gaagaacacc
     4561 agcaggaaca tcattacgtc gatcattgga atcagctcga tacgcgcgct gcgcttcggc
     4621 tcgtcatgcc agaacatcct gccaccccca tattcagcct tgaatcagta gcctgacagc
     4681 agttagtcag actttatgca aaacgggggc cattctgtca gcaaaatgtt acctttccgt
     4741 ggcatcaaca taaagctttt gtgaatccat cgtggcgcga acgcgtcttg ccacccacca
     4801 ccgtcgcgag acaatcccgg ctagcgccgt gcgctgtccg cctgcccatc gggtactgcg
     4861 catccgtatt gcttcgttca cctcgaacgc agtacaatct ggactatttc gctccaactt
     4921 ttgtattcca ccgatgaaca gagaaaaccg gttgcagggg acgctgtacc gccccgattt
     4981 cgaacaggac agttgtggtt tcggcctgat ggcacagctg gatgaccagc ccagccactg
     5041 gctcgtgcaa accgccatcg attcactggc ttgcctgacc caccgcggag cggttgccgc
     5101 tgacggcaaa tccggcgacg gctgcgggct gctgttcagg aaaccggacg ctttcctgcg
     5161 cgccgtggcg caggaaaacg gcctgcaact caatcgtcac tatgctgccg gcatcgtgtt
     5221 cttccatccg gacgcggcac agacggaaca tgcgcgccag accctgaacg gccacctcgc
     5281 cgcccagggt ctcgacgtgg cgggctaccg cacgctgcca gtcgatccgg gcgcatgcgg
     5341 cgaatacgca ctggccaccc tgccgcattt cgaacagata ttcgtcaact gcccggacgc
     5401 cctcgacgcc gagaccttcg agcgccggct gtacatcgca cgacgcctga cggaaaaagc
     5461 gctcgtcgac gaccgtacct tctacattcc cagcctgtcc ggccatgtcg tgctatacaa
     5521 gggtctggtc atgccggccg ccttgccggt gttctatccc gatctggctg acacacgctt
     5581 tgcctcgtcg ctggcggtgt accaccagcg tttttccacc aatacctggc ccgaatggcg
     5641 gcttgcccag ccgttccgca tgcttgccca caacggcgag atcaacaccg tgcagggcaa
     5701 ccgcaactgg tcgctggcgc gcgaacggaa attcgccacc ccactgatcc ctgacatgga
     5761 cgctgtgcgg cccatcgtgc agcgcaacgg ctcggattcg tgcagcctcg acaccatgct
     5821 cgaagggctg gtcatgggcg gtgccggcct gttccgcagc ctgcgtatgt tgattccgcc
     5881 agcgtggcag aacgtgccgg aaatggatgc cgatctgcgc gcgttctacg aatacaactc
     5941 gatgcacatg gaaccgtggg acggaccagc cggcatcgtg ctgaccgacg gccgctacgg
     6001 cgcctgcctg ctcgaccgta acggtctgcg tccggcacgt tacgtcctta cccgcgatcg
     6061 ccacttcacc atcgcgtccg aagtcggggt gtggaactac cggcctgaag acgtggtgcg
     6121 caagggccgc atcaaacccg gccagatgat cgccgcagac ctggccaccg gccagatcct
     6181 gatgccggca gacatcgagg cgcaactcaa gcacgcccat ccgtaccgcg agtggttgcg
     6241 ggaatcgcgc cgactcgagc tgggcatgga acaggacggt cagcttgctg ttcctgccgc
     6301 cgatcttctg gccacccggc agaaactgtt cggactcacg gtcgaggaac gcgaacagat
     6361 cctgcgcgtg ctcgccgaag aaggacagga agccgtcggt tcgatgggcg acgacacgcc
     6421 aatggccgtg ctgtcgcagc gggtgcgctc gcctttcgat ttcctgcgcc agcagttcgc
     6481 ccaggtgacc aatccgccca tcgacccgat ccgtgaagcc gtggtgatgt cactcaatac
     6541 ctgcttcggc cccgaacgca acctgttcga ggaaacgccc cagcacgcgc gtcgctatga
     6601 agtgccttcg ccgctgctgt cgcatgacgc gttcatcctg ctcaccacgc tcaacgacgc
     6661 cggataccgc agccacacgc tcgaactgaa ctacgacccg aaaacgacca gtctgcgtgc
     6721 cgccatcgag cagcttgccg cagacgccgt gcaggcggta cacgccggca gcgtcattct
     6781 tgtgctgtcc gaccgacgca tctcccagga tcgcctgccg atccacgccc tgtttgccgt
     6841 tggcagcgta caccacgcgc tgatccgcgc cggtctgcgc tgcgactgca acctggtcgt
     6901 ggaaaccggt gccgcgcgtg acccgcacca gatcgcctgc ctgatcggtt acggcgccac
     6961 cgccgtctac ccttggctgg cctatgaaag catcggcgca ctggtgcgcg acggcgaaat
     7021 cagcctcaag caggaaaagg ctctgaccaa ttaccgcaaa ggcatcgaca agggactgct
     7081 caaggtattg tccaagatgg gcatctccac tgtggccagc taccggggag cacaactgtt
     7141 cgaagccgtc ggactgcacg aggaagtcgt cgaactgtgt ctggccggca cggtgtcgcg
     7201 catctccggc gccaatttcg ccgatttcgc cgccgaccag caaacgctgg cgcgcgcggc
     7261 atggaacccg gcacgcccgg tcgcccaggg tggtctgctc aagttcgtcc atggcgaaga
     7321 attccatgca tacaatccgg atgtggtgct gcagttacaa caggccgtaa agaccggcga
     7381 ttacgcgctg tatcgccgtt atgccgatct ggtcaacggt cgccagatcg ccatgctgcg
     7441 cgatctgatg aaactgcgcg acgacgtcac gcccatcccg atcgaagaag tggaaccggt
     7501 agaggccatt ctcaagcgct tcgattcggc aggcatgtca ctcggcgcgc tgtccccgga
     7561 agcccacgaa gcgctggccg aaggcatgaa ccgtatcggc ggccgctcga attccggtga
     7621 gggtggtgaa gatccggcac gctatggcac gatgcgcatg tcgaagatca agcaggtggc
     7681 atcgggacgc tttggagtca ccgcacacta tctggtcaac gcggaagtac tgcagatcaa
     7741 gatcgcccag ggcgccaagc ccggcgaggg cggacaactg cccggccaca aggtgtccga
     7801 aaccattgcc cgcctgcgtt gcgccaaacc cggcatctcg ctgatttcgc caccgccgca
     7861 ccatgacatc tactcgatcg aagatctggc gcagctgatt ttcgatctca agcaggtcaa
     7921 cccgcaggcg ttggtgtcgg tgaagctggt tgctgaacct ggtgtcggca cgattgccgc
     7981 cggcgtggcc aaggcctatg ccgacctcat caccatttcc ggctacgacg gcggcaccgg
     8041 cgcctcgccg ctcaccagcg taaagtacgc cggcacgccc tgggaacttg gcttgtccga
     8101 agcgcagcag gtactgcgtg ccaacggcct gcgtggccgc gtgcgcgtgc agaccgacgg
     8161 cggcctcaag accggtcacg acgtgatcaa ggcagccatc ctcggcgcag agagtttcgg
     8221 tttcggcacg gctccgatga tcgcactggg atgcaaatac ctgcgcatct gccacctcaa
     8281 caactgtgcc accggcgtcg ccacgcaaga cgaacgcctg cgcagccagc atttcatcgg
     8341 cttgccggaa atggtggtca acttctttac ctttgtcgcc atggaaaccc gcgaatggat
     8401 ggcgcgtctg ggtgtgcgca cgatggccga actgatcggc cgtgtcgacc tgatgcgcat
     8461 cgaagccggc gacaccgaac gccaggctcg gcttgatctc cgtccactgc tggaacaggg
     8521 tggagtgccg gccagcgaac cgcaatactg catcaccgac tccaacccgt cgttcgacaa
     8581 gggcgaactt gccgaacaga tggtacgcga tgcacgcatg gccatcgaca gccagacgcc
     8641 cgcagaactg cactatgccg tccgcaacgt gcaccgctcg atcggtgcgc gcctgtccgg
     8701 tgaaattgcc cgcaaacacg gtgcagcagg actgccgcca ggctgcctga aggtgcacct
     8761 gaccggatcg gccggccaga gcttcggcgt atggaaccac aacggcctcg aactgctgct
     8821 ggagggagat gccaacgact atgtcggcaa gggcatgtcc ggcggtacca tcgccatccg
     8881 cccgccagcg gaatccacct ttgcctcgca cgaagccgtg atcatcggca acacctgcct
     8941 gtacggcgcg accggcggcg aactgtttgc cgccggggtg gccggcgagc gcttcgccgt
     9001 acgcaattcc ggcgcctttg ccgtcgtgga aggcaccggc gatcactgct gcgaatatat
     9061 gacgggcggc tgcgtgctgg tactgggcga aaccggcatc aacttcggcg ctggcatgac
     9121 cggcggcttc gcgctggtgt acgatgagca ggacaggttc gccaaccgct acaacaacga
     9181 gctgatcgat atccatcgca tccacactga aaccacggtc gagtaccggc atttcctgcg
     9241 cagtcgcatt gaaatgcacc tgcagcgcac gggcagcgca cgggctcgct ggatactcga
     9301 tcatttttcc gacatcaccg gaaaattctg gctggtcaaa cccaaggccg ccacgctgga
     9361 cagcttgctc aaggactgac gggcgacaga catgcatcaa aagacgagcg cacccgttat
     9421 gctacagaca gtcagcgcac ccggcggcct gcgccgccac ggccgctgaa gaaccaggac
     9481 acgacacatg gctgatgtat tccagtttct caacctgcca cgccaggatg gcgccaagac
     9541 tccggcggcc gaacgccgca tccagttccg cgaaatctat gcgggcatgg atgcctctca
     9601 ggccggcggc caggcaggtc gctgcctgtc gtgcgggaat ccgtactgcg aatggaaatg
     9661 tcccgtacac aactacattc ccaactggct tcagctgatc agcgaaggca aactgtttga
     9721 agctgccgaa ctctcgcatg ccaccaactc gctgcccgag gtgtgcggcc gcgtctgtcc
     9781 gcaggatcgc ctgtgcgaag gcgcctgcac gctcaatgac ggttttggcg cagtcaccat
     9841 cggccagatc gagaaattca tttccgacag cgcctttgcc gctggctggc gtcccgacat
     9901 gagccgcgtg gtggccagcg ggaaacgggt ggccgtgatc ggcgcaggcc cc
//
LOCUS       DAIANL010000014        11070 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_421195, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000014 DAIANL010000000
VERSION     DAIANL010000014.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 11070)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11070)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11070
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421195"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2080 a         3101 c         3637 g         2252 t
ORIGIN      
        1 gttaccgtgg cgaccggccc atcctgctgg cggtcacgtt actgaccagc atgggtgagg
       61 ccgacatgca gatgatcggc ctgtccggta cgccggcaga ggcggtgctg cggctggcgc
      121 ggctggcgca tgcctgtggt ctcgatggtg tggtgtgctc ggcgcatgaa gccgcattgt
      181 tgcgcgagga catcggcacc ggctttagcc tggtcacgcc cggcattcgt ccggccagtg
      241 ccgccagcga tgatcagcag cgcgtcatga ccccggctgc cgcgcagagc gccggagcgg
      301 attacctggt ggtcgggcgc ccggtcacgc atgcaccgga cccgttgcag gccgtgctgg
      361 aaattcgcaa tcaactgatt tcatgatacc gcaggagtaa aacgatgaaa gttaccgtgg
      421 tggggactgg ctacgtcggt ctggtcagcg gagcctgtct ggcggaggtc ggcaacaatg
      481 tgatgtgcct cgacgtggat gcggccaaga ttgccgtgct caagcagggt ggtatcccga
      541 tctacgagcc gggcctggaa gacatggtca ggcgcaatgt ggccgccggc cggctgcatt
      601 tcaccactga cgtggaagcg gcggcgctgc atggcgtcat ccagttcatt gccgtaggta
      661 cgccacctga cgaagacggt tcggccgatc tgcagtacgt ggtggcagcc gcgcgcaaca
      721 tcggaaaatt catgaacgag taccgtctgg tggtcaacaa gtccacggtt ccggtcggca
      781 cggcggacaa ggtgcgtctg gcggtacagg aagaactgga ccggcgtggt gtggcgcatg
      841 aattcaacgt cgcttccaat ccggagtttc tcaaggaagg cgcggctatc gacgatttca
      901 tgcgtccgga ccgcatcatc gtcggtaccg acgatgaacg tgccaccgaa ctgatgcgca
      961 agctgtatgc gccgttccag cgcaatcacg atcggctcat cgccatggac gtgcgctctt
     1021 cggaactgac caagtacgct gccaatgcca tgctggcaac acggatttcg ttcatgaacg
     1081 aactggcgat cctggcggaa aaacttgggg ccgatatcga aaacgtgcgc cagggcatcg
     1141 gttcggacat tcgtatcggt tatcacttcc tctattccgg ctgcggttac ggcggttcgt
     1201 gttttcccaa ggatgtgcag gcgttacggc gcaccgctgc cgagaacggc gtgccgatgc
     1261 gcgtgatcga tgccgtggaa gcggccaacg aggcgcagaa gcacctgttg ctgtcgaaaa
     1321 tcatcggtcg tttcggcgcc gacctctccg gcatgcgctt tgcgctgtgg gggctggcgt
     1381 tcaagccgaa taccgacgat atgcgcgagg cgcccagtcg cgtgctgatg gaaggtctgt
     1441 ggcagcgtgg tgcgcatgtg gttgcgtttg atccagccgc aggacacgaa acgcgacgca
     1501 tttacggtga acatccgcag ttgacgctgg tggatacgcc ggaagctgcg ctggcaggcg
     1561 ctgacgcgct ggtgatcgtg accgaatgga aagtgttccg ttgtccggat ttcgccttta
     1621 ttcaggcgtc gctgaaacaa ccggtgattt ttgacggtcg caatctgtat gacccgcaac
     1681 aggtgcgcga actgggcatt gaatatttcg ccattggtcg tcgatgagcc gtccgcaatc
     1741 ttttgatgcc gggcgtctgg ctgcagcacg catcctggtt gtgggcgatg tgatgcttga
     1801 ccgttactgg tttggcgatg tggcacgcat ctcgccggaa gctccggttc cggtggtgaa
     1861 aatccaccgg caggaagaac ggctgggggg cgccgccaat gtggcgcgca atctggctgc
     1921 gctgggtgcg catggtacgt tgctgtcggt gatcggcgat gatgaagccg gccaggccat
     1981 cgaacgcctg ctgggggagg cgggcatcga agcccgtctg catcgtgacc cggtgctgga
     2041 taccatcatc aagttgagag tgatcggccg gcagcaacaa ctgttgcgca tcgatttcga
     2101 aaccgaaccg agccaggacg tgctcagcga caagctggcc gaatttcacc tgttattgcc
     2161 gcattgcgac ctgctgctgt tgtctgatta tggcaaaggc gggctggcgc acgtggccag
     2221 catgatcgag gccgcccgtg cacggggaat tccggtgctg gttgatccca aaggggagga
     2281 ctatgcccgc taccggcatg ccgatgtgct cacgcccaac ctggccgaat tccgtaccgt
     2341 ggccggtggc tgggacagcg aacaggcatt ccgggccaag gcagaagcgt tgcgtcgttc
     2401 acttgaactg cgtgcgctgc tcatcacccg cagcgaggcg ggcatgacgc tgttcagtgc
     2461 cgagggtgtc agccaccatc cggcgctggc gcgcgaagtc tacgatgtca gcggtgcagg
     2521 tgatacggtc atcgccacac tgggtgccat gcttgccgcc ggcagcagcc tggacgacgc
     2581 ggtagactgg gccaaccgcg ccgccggaat cgtggtggga aaactgggaa ctgcggtggt
     2641 gacgcagcac gaattgttcg ccagcgactg acccaggcac gacattgcac agtcgaaccc
     2701 ggcgaaatgc cgggtttttt ttggcctgtt cgaccgattg ccgcgacgcg cactgttcga
     2761 taatgaaacc gtatcgctcc gtgcaggggc ggcgaaggga tgacaggcga tggctgaaga
     2821 cagcgatctc gaaaaaacgg aacaggcttc gcagcagcgt ctggacaagg cgcgcgagga
     2881 aggcgatgtt ccccgttcac gcgaactggc aacttttgtg ggtctgctgg cttcgggcgg
     2941 tggcgtgtgg ttcatgggca gttcgctggt gcatgccctg gaagcttcgc ttgccagcgg
     3001 aatgcagttc gagcgtgccg acgcggtcga cttttccatg ctgttcggtc acctgagtca
     3061 tggcctggtt caggtggcgt tggcattcgc gccgctggca ggcctgctgt tgctggtggg
     3121 cgtggttacg ccggccctga tcggcggctg ggtcatcagt cccaacgtca tgcagttcga
     3181 tgtcaaccgc atcaatcccg ttgaagggat tgccaaaatg ttcactgtgc gtacgctggt
     3241 tgaactggcc aaggccatca tcaaggccgg tctggtcggg atcgtggcat ggacgatgat
     3301 catgcatgaa aaggaagcgc tgctgtcgct gggagtggag ccgctgcgcg ctgctgtagc
     3361 cgattccggt cagctgatct ggaaagccta cctggcgatg acgatggcgc tgggcgtggt
     3421 ggcattgatc gatgtgcctt accagttatg gaattatgcc gaccgtcaca agatgacacg
     3481 ccaggaactg cgccaggaag cacgtgagtc agagggtgat ccgcaggtgc gcggacgcat
     3541 ccgtgcgcaa cagcgtgaaa tggctcgccg ccgcatgatg gccaacgtgc ccaaggccga
     3601 cgtggtggtg accaacccga cccattatgc tgtggctctg caatattccg accagggttc
     3661 gcgtgcgccg atggtcgttg ccaagggtgc cggagaagtg gcggcacgta tccgcgaact
     3721 ggccgatgag catggggttc cgcgcatgga agcgccggcg ctggctcgtg ccctgtatcg
     3781 ccatgtcgaa ctggagcggg aaattcccga tgtgctctat gccgcagtgg ctgaagtgct
     3841 ggcttatgtc tatcagttgc gcagctggcg cagccgcggc ggacagtacc cggtcatgcc
     3901 gtcatatatc gaggtgccgc ccggaatgga tccactcgaa agcgaaacag aacccgccgc
     3961 cgctgcagaa ggagatgacg catgaatgcc ctgaaatggc ctgcatggtt caagccaggc
     4021 gcatcgttca tgattgcgcc cgccatcatc atcctgttgc tggcaatgat ggtgctacca
     4081 ttaccgccct ttgtgctgga cgtgttcttc agcttcaaca ttgccctggc gatcattgtg
     4141 ctgctgacca gtctgtatac cgtcaagcca ctcgacttcc tcgcgtttcc gaccgtgctg
     4201 ctggtcagta ccatgctgcg gctgtcgctc aatgtggctt cgacgcgggt ggtactgacc
     4261 catggtcaca ccgggccgga cgcggccggc aaggtgatcg agtcattcgg ccattttctg
     4321 attggcggca actacgccgt tggcatcgtg gtattcatca ttctcaccat catcaatttc
     4381 gtggtggtga ccaagggtgc cggacgcatc gccgaagtcg gtgcgcgctt cactctggac
     4441 gcgatgcctg gcaagcagat ggccatcgat gccgacctga acgccggtct gatcggtgag
     4501 caggatgcac gccgtcgccg cagcgaggtg gctcaggaag ccgagttcta cggggcgatg
     4561 gacggtgcga gtaaatatgt gcgcggcgat gccatggcgg gcattctggt cacggtcatc
     4621 aatatcgtgg gcggattggt ggtcggcatg gtgcagcatg atctgagctt cgacgtggcg
     4681 atcaagaatt atacgctgct cgcgattggc gacgggctgg tggcgcagat tccatccctg
     4741 atcatttcca cggccgcggg tgtcgtggtt tcgcgcgtgg ccagcgaaca ggatattggc
     4801 ggacagctca ttgcccagtt gttcgccaag ccagtggtgc tttatatcgc ggccagcatt
     4861 ctcgccggta tgggcatcat tcccggcatg ccccatctgg cgtttctgtt gttggccagc
     4921 ctgattggca gcgcggcctg gcttcaggaa aggcggctgc gggtcgtgcc tgaggaagcc
     4981 ccggctgcac ctgcgccgag cgctgagtcc gaagaagcaa catggaagga catcattccg
     5041 gtcgatacgc tggggctgga agtcggctat cggctgatcc cgttggtcga caagggacaa
     5101 ggtggagaat tgctgcgtcg catcaaggga atccgcaaga aatttgccca ggaaatcggt
     5161 ttcctgccgc cgtcggtgca tatccgtgac aacctggaac tcaagccggc ggcctaccgc
     5221 atcagcctga aagatgttga aatcggccag ggcgaagctt atgcgggcca gttgatggcg
     5281 atcaatcccg gtgtggtcaa tggcacgctg ccggggctgg ctaccaccga tcctgcgttt
     5341 ggtctgccag cgatctggat cgacgcagga atgcgcgacc aggcccagtt gatggggtac
     5401 accgtagtgg atgcctccac cgtgatcgcc acccacatga atcatctgat ttcgatgcat
     5461 gccgcggagt tgctcggccg gcaggaagtg cagtcgctgg ttgatcatct ggctgccgaa
     5521 tcgcccaagc tgatggaaga actggttccc aagatgatga cagtgtcgca attgcagaaa
     5581 gtgctgcaga acctgctggt cgaaggtgtg cacattcgtg atatgcgcac cattgtcgag
     5641 acgctggccg agcatgtcgc tcgcgttcag gatgccaccg aactgactgc attggtgcgc
     5701 atcgctctcg gccgtgccat tgtgcagcag gtgtatccgg ccacgcagga agttgccgtc
     5761 atggcgctgg acacccgtct ggaacgtctg ctgatgcagg cagtgcaggc tggtggtgag
     5821 caggcggcca tcgaacccgg cctggccgag acgttggtca accaggtcag ccgcgcaacc
     5881 gtgcaacagg aaagccaggg cctgccggca gtgctcctgg tgccgccggc gctgcgctgg
     5941 ttgctggcgc ggttcctgcg ccgttcgatt ccgcagctga aggtgctgtc gcacgctgaa
     6001 attcctgatt cacgcaacat tcgggtgacc aatctgatcg gaggtcagac atgaacgtac
     6061 aaaaattcat tgccgccaat tcgcgcgagg cactgcgcaa ggtgcgcgac gcgctcgggc
     6121 ctgaggccat gattctttca aatcgcagca ttgccgaggg tgtggaggtg gtggccctgg
     6181 ccgccgagga catggctgcg ctgacggttt cgtctgccga ccgtgacatg caacccgatt
     6241 tcgactgggg gcgcgcaccg cagcgtgaaa cggtgcaggg ccgcgctgcg gccctgcaga
     6301 cgcgcgccac cccggtgctg gccgatacct ggagtgagga tgaacctgag gcgacgccgg
     6361 cctcggtgcg ggaaatggcg aacatgatgc aggaaatccg tgccatgcgc ggcatgctgg
     6421 aaattcagct ggccgaactt gcctgggacg gccagcgcag ccgcgagccg catcgcgctc
     6481 agatcctgcg tgatttgctg acggccggct tcagccctgg actgtcgcgc cacctgctgc
     6541 agcatctgcc ggaagtggct aatgccctgg aaggcatgca atgggtcaag gccgcgctgg
     6601 cacgcaatct caatgtgatg cacaacgagc ttgacgtact ggaacgtggc ggggtgttcg
     6661 ccctggtcgg accgaccggg gtgggcaaga cgaccacgac cgccaagctg gctgcgcgtt
     6721 gtgtgatgcg ccatggcccc aacaagctgg cgctgattac caccgacggc taccgtgttg
     6781 gcgcgcacga gcagttgcgc atctacggca agattcttgg cgtgatggtg cattcggtgc
     6841 gcgacgaaag cgatttgcgc attgcacttg ccgaactgcg tggcaagcat accgtgctca
     6901 tcgataccgt tggcatgaac cagcgcgacc agatggtggc cgagcaggcc gccatgcttg
     6961 ctggcgccgg tgtggaagtg cagcgcctgc tgtgtctgaa tgccacctcc actggtgaga
     7021 cgctcaacga agtcgtgcgt gcctatcagg gcagcgggct ggccggttgc attatcacca
     7081 agctcgatga ggcagcgacc atgggcaatg tgcttgacgt cattattcgc cacaagttgc
     7141 gtctgtatta tgctgccaat ggccagcgcg tgccggaaga cctgcacttg atgagccggg
     7201 agctgctggt cgatcgcgcg tttcgtgtgc gccgcgaaat gtcggcgttc cgtttccagg
     7261 acgaggaact gtcgttgatg gtgtcagctg cccgtggcaa tgacgccgaa cgtgaatttt
     7321 cggaggtgcg ccttggctga ccagattatg gatcaggcgg cgggtcttcg ccgcatgctg
     7381 gcggttcccc gtccgcgcag aatcacgctg ctgtccacgt tgcctgcgcc ggagcgcaat
     7441 gtcgtgttcg ataatctgtg cgccggcatg gcgcatgccg gcagcaattg cctgctggtc
     7501 gacgccaggc gccatccacg tatgggcacc catctgggtg tgatgcgcag gccagccttg
     7561 ctcgatgcag cccatggacg caacccgctg gaagattcgg tacagcggct cgatgcgggc
     7621 tattcgtttg ccgtgttgca gggtggcaag catctggcag ggcggccgcg gtatatggat
     7681 gaggcactgt gcagaatttt cgatcgcctg tcgtcgcgtt atgacagtgt attcatcgat
     7741 gccgaactcg acgaatacga cagtctgccg gcgagcgtac ttgatcgatc gcgcatggta
     7801 gtgcatatcg gcgccgatgc ggaatcgatc aagtctggtt atgcgatgat caaacggctg
     7861 gtgacgaatg tgggcactgg cgagtacggc gtactggtca gtggcacgga accggctcgc
     7921 gccgggctga tttatgctaa tcttgcaaaa acggccagcc ggtatcttgc ggtatcattg
     7981 caggatatgg gatcgattcc gccggacgat catcttggcc gtgcagccag actgggccgt
     8041 tcggttattg acgcctttcc gctggcgctg gcatccgcgg ctttccggcg tattgcggga
     8101 caattgagcg tttcttgaaa tatgtacacc gtcagcggaa aagccgacaa aaaccagata
     8161 cttctggaac atgcgccgct ggtgaaacgg ttggcgtacc aactgaaggc gaggctgccg
     8221 cccagtgtcg aggttgacga tctgatccag gccggcatga tcggcctgct tgacgcgatc
     8281 aaccgttacg aggacacgca cggggcgcag ttcgagactt atgccgtgca gcgcatccgt
     8341 ggttcgatgc ttgacgaact gcgcggcatg gactggctgc cgcgcaatgt ccgtcaggac
     8401 atgcgcaaga tcgaaaccgc gatgtccaat ctgcagcagg aactgggccg cccgccgagc
     8461 gaagccgaga ttgcgcgcgc gatgaaactt accctggctg aataccagga aaaactctcc
     8521 gatggcggtg gacaccaact cgtctattac gaagactatc aggacgaggg ggacgaacgt
     8581 gacaattttc ttgaacgcta catggtggac gaaaacggcg atccgctgtc gagcctgctc
     8641 gaggacgact tccgccgcgc cctgatcgag gccatcgaag atctgccaga gcgcgagaaa
     8701 atcctgatgg gcctgtatta cgaacaggaa atgaacctga aagaaattgg cgccgtcatg
     8761 ggtgtgtcgg aatcgcgcgt gtgccagctg cacagtcagg ccatcgcgcg cattcgttct
     8821 tcattgagga gcctggcatg gactgggggg ggttagccgg gcttgcgctg gcatttgccg
     8881 gcattctggt gtcttacggg ctggacggtg gccatgtgct gtcgttgctg cagcctgcgg
     8941 cattcatgat cgtgttcggt ggcacagtag gcgccgtgat cacgcagacg cggttgccgg
     9001 tattcgtgcg tggcgtaaag atggcgcgct ggctaatgca gccgccggat gaccagcgcc
     9061 ggatgctgag tgccgatatc gtacgctgga gtcacagtgt acgccgtgat ggtctgctga
     9121 gcctggagcc ttatctgcgt gcggcccggg atccgtttgt cagcaagggg ctgcgcatga
     9181 tcatcgacgg tatcgatgaa aatcagttgc gccagattct cgatgccgag atcgcagccc
     9241 tcgacctgta ccatcgcacg gccgtcaaga tctgggaatc tgctggcggt tattcgccga
     9301 ccatcggtat tctgggggcg gtgctcggac tgattcgcgt catgcagcat ctgtcggacc
     9361 cgaccaagct gggcggcggc atcgccgtgg ccttcgtggc tacggtgtac ggtgtcggtc
     9421 tggccaatct ggtctacctg cccatcgcca accggctcaa ggcggccatc acccagcaga
     9481 tccgccagcg tgaactggtc atggaagggt tgatggcaat cgcacacggc gagcacccgc
     9541 gcgtgatcga agaccggctt gcagcctatc tggagtaaat catggcgcgt cggcatcggg
     9601 agcgggaaga agaggagaat ctcgaacgct ggctggtgtc ctacgcggat ttcatcacat
     9661 tgctgtttgc attttttgtt gtgatgtatg ccatttcggc tgtcaacgaa ggcaaatacc
     9721 gtgtgctgtc caatgcgctg ggcgtggctt tcggccgggt ggttgtggcg ggcacgcaga
     9781 gccatccgat tctggtcaag acggtgatcg tcaaggagaa gccacggccg gtgcataccc
     9841 agcgcgatgt ggaggctgaa cagatgaata ccgtggcccg cgatctgtcg cacacactcg
     9901 acccgctggt gaaacagggc atggtcagag tcagccagtc ttcgcgcgga gtggatgtgg
     9961 agatcaatgc cagtctgctg tttccttcgg gtgcggctgg attgtcccag gattcgaagc
    10021 gtgcgctgca ggcgattgcc gcggtgctca aagacaagcg ttacgatctg gaaatcaatg
    10081 gttatactga tgaccagccg atccgcagcg ccgtgtttcc ctcaaactgg gagctgtccg
    10141 cagtgcgtgc cagcagcgtg gcgcgcgaac tggtggcgca gggcatcgcg gaagaccggc
    10201 tgaaggtggt cggcaatgct tccaatcatc ccatcgctcc gaacgacaat actgcagacc
    10261 gtgcgcggaa ccgtcgcgtc gagatcgtcg tcctcgcgcc acaatacgaa gctgcacggg
    10321 aaaccaccta taccacgggc tctggaaaat agcgggtttc ggagaataac cctgttcaca
    10381 tccgtgttga agcgcggttc acccatttgg tacgatggac attttgccgc caatcgccat
    10441 gcacagtgat ctgaaaaccc ttgagttcga cggcattcgc cggattctgg cgcgcctggc
    10501 gctgaccccg tacggcaatg atgctgccca taacctcgaa ccggcgcctg acgccaccgt
    10561 ggcacgtggc atgcggcagt cggtcagcgc agcgaggcag ctcgtcgatg ccggtatgct
    10621 gccgcggttg ggcgaggtgc cggatgtgcg cgctgcgctg cgtcaggctg gccaggccaa
    10681 tgctgcattg tcagcacagg cactgcacaa tatccgtctg gtttcggccg ctgcggcgaa
    10741 gctggcgacg ctgtgtgccc agtttcccgg gctgatatca cagccggatg ctttggtcgc
    10801 accgcgcgac tgggaggccg cattggaacg cagcctgcag ccgcaagggc ggctgcgcga
    10861 agatgcgagt ccggcgttac aggaattgca tctcgcgttt cgtgcacagc aggcggaagt
    10921 cgaccgcaaa ctgcgcgaga tcatgggcga tgccagatac catgggctgt tcgatggacc
    10981 gcacgcgttg cagtggcatg gcgcacgtgc ggtgctggcg gtgcgcagca acaccgcgga
    11041 acgcttgcgt ggcgtgcggc gtggcagtag
//
LOCUS       DAIANL010000015        18043 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1035740, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000015 DAIANL010000000
VERSION     DAIANL010000015.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 18043)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18043)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18043
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035740"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3234 a         5250 c         5957 g         3602 t
ORIGIN      
        1 tgcatgtccg ccatataggt cttcaccttg gcgttgtgcg gcatcaggtt gctggcgaca
       61 aaaaactttt tgccgagcgc atgcgcttcg gtgatgccct gcgccagttg ttcgaggccg
      121 aattcattgt tgcgcgcgcg cagcgaatag cgcggttgtc cggcatagac tgcatcggcg
      181 ccgaagcgga aggccaggtg cattttttcc aggctgcccg ccggggcgag cagttcggtc
      241 gtggcgggct tcattcgatg cgttgcgcga gttcggcagc catgccgatg tagttggccg
      301 gcgtgagtgc cagcagacgc tcgcgttcgg cattcgggat ggacaattga cggatgaacg
      361 catgcaggtt gtcgcggttg atggtgccgg ccttgccgcg cgtcagttcc ttgagctgtt
      421 cgtaggcgtt ctggatacgg tagcgacgca tcacggtctg gatggcttcg gccagcacct
      481 cccagctgcg ttccaggtct tcgtcgagcc gcagcgcatt ggtctgcaat ttgtccaggc
      541 cgcgcaagca ggaatcccag cccagcaggg catagccgaa tgacaccccc atgttgcgca
      601 gtacggtcga atcggtcagg tcgcgttgcc agcgggaaac cggcagtttc tccgccagat
      661 gacgcagcac ggcgttcgac aggccgaggt tgccttcggc attctcgaaa tcgatcgggt
      721 tgaccttgtg cggcatggtc gaagaaccca cttcgccggg cttgagcttc tgcttgaaat
      781 aaccgagcga gatatagccc cagatgtcgc gcgacagatc gatcagtacc gtattggcgc
      841 ggcttaccgc gtcgaacagt tcggccatgt agtcgtgagg ctcgatctgg atggtgtagg
      901 cgttgaaatg cagtcccagt ccggtgacga agcggcgcgc gaaaccttcc cagtcgaagt
      961 ccggccatgc ggcgaggtgg gcgttgtaat tgccgaccgc tccgttgatc ttgcccatca
     1021 gtgccacatc ggcgatctgt gcctgctgac gctgcaaccg ccaggcgatg ttggccattt
     1081 ccttgcccag cgtcgtaggc gtggccggct gtccatgcgt gcgcgacagc atgggttgaa
     1141 aggcattgtc atgcgcgagt tccaccaatc gggcggaaac ccgttcgagt gtcggcagca
     1201 gtacgtcctg gcgtgccttt ttgagcatca gtgcgtgcga caggttgttg atgtcttccg
     1261 aagtgcaggc gaagtggatg aattcgccgg agctggcgat ttccgggttg ttgctgaaat
     1321 gttcgcgcag gaagtattcg acggccttga catcgtgatt ggtgttgcgt tcgatggcct
     1381 tgatgcattc ggcatcctgt tcgctgaact ggctggccag ttcgtccagt tcggcgatgg
     1441 tggcggtgga aaacgggccg atgccttcga gcccgggttc ggcggccagt gccttgagcc
     1501 attcgatttc cacgatgacc cggtgatgga tcagaccgaa ttcgctgaac atcgggcgca
     1561 ggatttcggt tttcgctgca taacgtccat cgagcgggga aagcgcggaa agcggggaga
     1621 ggtccatggc gatcgtttcg tgttgtggcg aaaggggcat tttacaggat tccatgcacg
     1681 ctggaatgcg gcatgacgcg gttttgcatg cctctgccgg catgtcggcc atgtcgcgct
     1741 tttgccggat gtccggtgct cgtggcacaa ttcggtctgt aaagacgaac tggcggcggc
     1801 aaccaggcat gaaactgaga atttttcgtt tcgatccgga ccggaatgat gagccggtcg
     1861 agcaggaata cgagatcgat cccgcgctgg ccgggccgat gctgctcgat gcgttgctgt
     1921 atgtgcgcga gcacctggac gcgagtctcg cgttgcgtcg ttcatgccgc gagggggtgt
     1981 gtggttcgga cggcatgaac gtcaatgggg tgaatgtgct cgcctgcatc accccgctgg
     2041 ccgagctgag cgagccggtg atgatccggc cgctgccggg tttgccactc atccgcgacc
     2101 tggtggtgga catggagcca ttgctgcgca attatcgcgc agtgcatccc tggctcgagg
     2161 ccgatgtggc cccggccgag cgcgaacggt tacagacgcc ggctcaacgg actgcattgc
     2221 aggcggctga cgagtgcatc cagtgtggct gttgcagcag tgcctgtcca tcgttctggt
     2281 ggcgtcctga tgagtttgcc ggaccggcgg cgctgcttgc ggcctggcgt ttcgtcgcag
     2341 acagccggga cgtgcaaacc ggtccacgcc ttgccggact cgcccccggc cggcgcctgt
     2401 ttggctgcca tggtgtgatg aattgcgcgg cagtctgtcc gaaagggctg aacccggcgg
     2461 cagcgattgg tacaatgaag cgctggctgc tcaaatcggg gcagcatcgg tgacgccagc
     2521 gctgaaccgg ctgcgctggc tgtgccggcg cggcatgctg gaactggata ttttgctgga
     2581 cgaattcctg cgcctgcatc acagcgaact ggatccgcgc gaacggcagg cctttgaacg
     2641 tctgctggcg ctggacgacg cggtattgtg gcagcacata caacagaacg gtggcatcac
     2701 caccgccttg aacaaggcga ttttttccga ggacccggca catgaccact cccaccaaag
     2761 cgatcctgac atttgacaat ggcgcggcgc ccatcgaact gcccgtgcag gcaggcacgt
     2821 atggcccgga tgtcatcgat atccgtgcct tgaaccagca cggttatctg acgtatgacc
     2881 cgggcttcat gtccacggcc agttgcacgt cgggaatcag tttcatcgat ggcgaggctg
     2941 gcgtgctcta ctaccgcggc tacccgatcg aacagctggt gcagcacgca gacttcctgg
     3001 aagtggcctg gctgctgttg ttcggcgaac tgccgttgcc tgcagaaaag gcggatttca
     3061 gccagcaggt gcgccgcaac gccatgctgc atgaccagat caacaacgtg ttccgcggtt
     3121 ttcgtcgcga tgcgcatccg atggcggtca tggtcggtgt caccggcgcc atgtcggcgt
     3181 tctaccatga ctccagcgac gtgttcgatg cggcgagccg tgtgcagacg gctgtccacc
     3241 tgatcggcaa ggtgccgacc atagcggcga tgtcgtacaa gtacaacatg ggcgaaccgt
     3301 ttgtctaccc gcagtaccaa ctcggttatg cggagaactt tctgcacatg atgttctcca
     3361 ccccgtgcga atcctacgaa gtcaacccgg tgctggcgcg cgcgctcgac cgcattttca
     3421 ttctgcatgc cgaccatgag cagaatgcct cgacttccac ggtgcgcctg gccggctcat
     3481 cgggggccaa tccttatgcc tgcatcgcgg ctggcatggc ttcgttgtgg ggaccgttgc
     3541 atggcggcgc caatgaggcg gtgctgaaaa tgctggcaga gattggcgac gtgtcgcgca
     3601 ttcccgaatt catcgagcgg gcgaaggaca agcagaatcc gttccgcctg atgggcttcg
     3661 gccaccggat ctacaagaac atggatccgc gcgcggcctt catgcgccag acttgccacg
     3721 aggtgctgga agaactggat ctggtggatg atccgctgtt ccgtatcgcg ctggaactcg
     3781 aacgtatcgc cttgcaggac gaatatttca tcgcccgcaa gctgtatccc aatgtcgatt
     3841 tttattcggg catcgtgttc cgtgcattgg gcattccgcc ttcgatgttt actgccatct
     3901 ttgcgctggc tcgcacggtg ggctgggttg cacagtggaa tgaaatgctg gccgacagca
     3961 gtcaccgcat cggccggccg cgccagcact ataacggcgc gacccggcgc gattacgttc
     4021 cagagcatct gcgcggcgca tgacgcagcc ggacacagcg atactgctcg ccgcttccgc
     4081 cgcatggctg gaagccgcga ctgccggcga accggctgcc agtgtctctg ccgtcgatcc
     4141 gctgcccgtg tcgtctgatg cccggtcgca ggccgatgcg cgtcaggtgg ccgtgctgcg
     4201 tctgatcaat gcttaccgct acctcggagt gcgtgctgcc gatcttgatc cgctgcggcg
     4261 catggacaat ccgccgcctg aagagctcga tccggcgcat tacggactgg ggagcgatga
     4321 cctgccgtgc gaattcgata ccggttccct ggctggtccg gcgcgcgata cgctggccgg
     4381 catcctccaa cgcttgcggc gcacctattg cggccggctc ggcatcgaat acatgcacat
     4441 ttccgatgcc ggccgcaaac gctggctgca gcagcgcatc gagtgcgata ccccaccggc
     4501 cgccgaggcc gggcaacgcc gccaatggct gcgctggctc accgaagccg aagtgctcga
     4561 acagttcatc cacacacgat acgtcggaca aaaacgcttt tcgctcgaag gcgcggaaac
     4621 cctgatgccc atgctgcact ggctcatgcg ttgcgcgtcg gagcagggtg tgcaggaagt
     4681 ggtgatgggc atggcacacc gtggccggat caacgtgctg gtcaacctgc tgggcaaacc
     4741 gctgcatgcg ctgttcggcg tcgggcggct tgacaatcat cgtgcccttg atggcgatgt
     4801 gcgctaccac cagggctttt ctgccgaact ggcaacaccg gcgggccggt tgcatctggc
     4861 gctggcgttc aacccgtcgc acctcgaaat cgtgcatccg gtggtgcgcg gctcggtgcg
     4921 tgcgcgtcag catcggcgcg gggatgcgga cggagtggaa gtgatgccgc tgctgattca
     4981 tggcgatgcg gccttttccg gtcagggcgt ggtgatggaa agtttcaaca tggcggaaac
     5041 gcgcggttac gataccggcg gcgccatcca catcgtggtc aataatcaga tcggctttac
     5101 cacgtccgat ccgcgcgaca cgcgctcctc gttgtactgc accgatgtgg ccaaaatgat
     5161 cgaggcgccg gtgctgcacg tcaacggtga cgatccggaa gctgccttgc gtgcggttga
     5221 aatcgcactt gaataccggc gggcatttcg caagaacatc atcatcgatc tggtgtgttt
     5281 tcgccgccat ggccacaacg agcaggatga gccggccgtg acccagccac tgatgtatca
     5341 gcgcatcgct ctccatcccg gcacgcgtac actgtacgca cgtgcactgg aagtgcaggg
     5401 agtgatcgga gcgcacgagg ccagggaact ggccgatctt tgccgtgaac ggctcgatgg
     5461 cgaggaaccg ctggtacgtc cggccgaatc gctgttctcg cgcagttttt cagcacgctg
     5521 gcaggcatat caccgcagcg actggcgtca gccggtcgat acagcggtgc cacctggccg
     5581 tctgcgcgag ctgggtgaaa ttctttgccg cgtcccgccc gggttcgtcg cccatgagcg
     5641 cttgcagcgc attcttgctg accggactgc gatgctggcg ggagaactgc cgctcgactg
     5701 gggcctggca gaaaatctgg cgtatgccag cctgctacgc gatggttatc cggtgcgtct
     5761 gtccgggcag gacagcggcc gcggtacgtt ctttcatcgc catgcggtat ggcatgatca
     5821 ggcgcgtgaa cggcgtgacg caggcgagta tgtgccgttg caacacatct atcccaatca
     5881 gccgcacttc atcgtggtcg attcgctgtt gtccgaactg gccgtgctgg cattcgaata
     5941 cggttatgcc agtgccgaac ccgatgaact ggtcatctgg gaagcccagt ttggcgattt
     6001 tgccaacggc gcacaggtgg tgatcgacca gttcatcgcc agcggcgaaa gcaagtgggg
     6061 ccggctgtgc gggttgacgc tgtttctgcc gcatgggcag gaagggcagg ggccggagca
     6121 ttcgtcagcg cgtccggaac gttacctgca actgtgcgcc caggataaca tgcaggtctg
     6181 tcagcctacg accgccgcac agatgttcca cctgttgcgg cgccagatgc tgcgtgtgta
     6241 ccgtaagccg ctgatcgtgc tcacccccaa gagcctgttg cgcgcccgtg ccgccagcgt
     6301 gccgctgtcc gcgttgactt cgggacagtt cgagccggtg ctgggcgacg acgccgtgcc
     6361 cgatacggca cacgtcacgc gcgtgttgct gtgcagcggc aaggtgtact acgatctgct
     6421 cggttcgcgt gatgaacgcg gccgcactga tactgctctg gtacgtgtcg aacagttgta
     6481 cccgtttccc gacgaagaac tgcgtgcggt gcttgcggcg tatccggggc tggcggaatg
     6541 cgtgtgggtg caggaagagc cgcgcaatca gggtgcctgg ccggagctgg aatggcgatt
     6601 acgtgcgttg ctgtcgcctg gttgtcgcct tcgtctggca agccggccgg aatcaccggt
     6661 gcctgcggcg ggctaccccg atgtatacca gaccagtcga caggcgctga tagatgaggc
     6721 atggtcatga aagtggatgt caagacacct gaactgtccg agtcagtggc ggaaggcacg
     6781 ttgctggaat ggaagtgcaa gcctggggac cgcgtgacgc gcgatgagcc actcatcgat
     6841 ctggaaaccg acaaggtcat tctggagatt tctgctccgg ccagcggcat tctgcttgaa
     6901 tgcctgcgcg agaacggcgc tacggtgacg ccgggcgaaa ccatcgcccg tatcgacacc
     6961 ggtgaatctg caccggcgca accgcaggcg accgttgcct tgccactggc gcccgcaccc
     7021 gcacctgcac ccgcacccgc acccgcacct gctgtcacag caggcgatgc gcttgcgcta
     7081 cctgttaatg ctgcccccgc tgccccggaa tccgggtcgg tgcgcgcgcg taccggagag
     7141 cgccgcgaac cgatgagccg gttgcgtcag cgcattgccg agcgcatgct gtccgcgcag
     7201 cagcaggcgg caatcctgac cacattcaac gaagtcaaca tgcaaccggt gcaggcactg
     7261 cgtgcccgcc accagcagga tttccagcgc gaacatggcg tgaagctcgg cattctgccg
     7321 ttcttcgtca aggcggtcgt gctggcgctg cgtcaatacc cgatcattaa tgcatcgatc
     7381 gacggaacgg atatcgtgta tcacgatgcc tgccatctgg gcattgccgt gtccacttcg
     7441 cacgggttgg tggtgccggt gctgctcgat gcgcagacgc tggaatttgc cgggattgaa
     7501 cgcagcatca gagatttcgc acaccgcgcc gagcagatgc aattgcagtt cgccgagctt
     7561 gagggcggta cgttcaccat cagcaatggc ggggtgtttg gctcactgtt gtcgacgccg
     7621 atcctcaacc cgccgcaagc cgccattctg ggcatgcatg ccatccagga ccgaccgata
     7681 gcggaacagg gtcaggtcgt catccgcccc atgatgtatc tggcgctgtc ctacgatcac
     7741 cgtctgattg atggccgcga agcggtgcag tttctcgtcg agatcaaatc agcgctcgag
     7801 gagcccgagc ggctgctgct cggcctctga gcgttcagcc gggttgtccg tccacgcgtg
     7861 cctgcaccag gatcggggcg tagatgcgca cgaatagcag gaaggcaaca atccatgcca
     7921 cttgcgccat agccagccac agcaggtaat gcgcactgat gatgacaggc aatacgacgc
     7981 ggaacacaac ggccagcacc agcagggtaa acactgtggt gagccgtttc ggtggctgct
     8041 ggatgttgcg tccagtgtga ccgagtgaca cccgcgccat catgccgacg gtcatgatgc
     8101 ccacgccacc cagcgcgatg gcatgcagcg ccagggtggg cgacagggac agcatcggtg
     8161 tgactgcctt gagcaggaaa ccggcggtga cgatggcata ccccagatac aggacccaca
     8221 gcaggggttt gctccagatg ccgcgggtat gccatccggc cagacggatg ctgtgcacga
     8281 cgcatagcag cagcgccagc aggctggtca gcagcggcag ctgtacgaag acatcggcga
     8341 tccagaatgc aagaaacaga tacaggttgg aacggtcgat ccaggcatgg ttggtcagct
     8401 gtaccgggta gccgacgccg cgttcgatga agaacggcag cacccgtcgc gccatgttga
     8461 acatcagcgc taccagcagg tacacgcccg agtacaggcc ccattcgacg ccatgacgca
     8521 gcacgccggc gataccgagc cagaaggcaa ggttcgccag tgcaagcaag accagcttgg
     8581 ctacaatgcc ggcctgctgt ttctgtcggg tacgcatgat cggcagcgtg accgcgacga
     8641 tgaacagcac gtcgaatgcc agatcgaaag tcatgggtac ggcaaccgaa tgtgtcatcc
     8701 agccggcgct gcgcgccgcc agccagagca gggtcaggcc cgccagcggg gcgccctgag
     8761 cggtgggaag accggtccag ttgcggacgg cagtgagcag gaatccggcg accacggcca
     8821 tgccatagcc gaatatcatt tcgtgcgcgt gccagtatat cggcagaaac ggcatgcgcg
     8881 gcagtgacca gccatatacg aacaccccga cccagagcag gatggcgatg ggagaataca
     8941 ggccggccag gaggaaaaac ggacggaaac cgagattgaa cagcgcgaac cggggtacac
     9001 ggcgtgcggg gtcctggatg gtgatcatcg ggcgactccg caaacggcag ccacatgtgc
     9061 agccggcagc aagatggtcg gtgcgataac gttgtgcggc atggtgaact cctggaaatg
     9121 catgatggtt cgcatggtag cagcaaccgg gcggtcattc attgattcag atcaagcggg
     9181 tacaggacag gattggtgtg cgcatcagcc cgggctgcct gcagccagcc tgatatacat
     9241 gcgggttgcc tgcaacaggt tccaccacag ccagccggcg gacagtacaa acgtgaatcc
     9301 tgccacatgg gccaacacgg gccagccgag gctggcgaac agcaacagca gcgcgatggt
     9361 gtgcaggcgc aactggcgac gtgagtcgga gtctgcaatc accagtttca ttccaggtgc
     9421 agtgttgcgt cgggccggat ggctttgcaa atggaaccag accagaaatg gtacgatctt
     9481 gtacagcatg ccattgacga ccgagatggc gaatccggcc aggtacagca tgccgagggc
     9541 aatcgggtag cgtgggtcgt caccgatgga cgtcacctgt ccggccagcc agagcaatgc
     9601 ggcagcgagc agactggcca tgccgatgcg ccagaattgc aggctgacgt ccggcagctt
     9661 gcgccggcga gcagcctgca agcggagcgt gaccagggca tacgatgcca gacaggtaac
     9721 gagtacgccg tgggtgcccg tggtgatcca ggccgccggt gcagaagcca gccatggcgt
     9781 cgcggaatcc agcgtcagcg cggcatagat cacgccaccg aaccagcggg tgactgctgc
     9841 cggataggcc ggggtgagct ggaacatggg gatcacttgc caggacacac ccatcaccag
     9901 aacaccgacc caaccgatga gtccccattg cgcgtgcaac gaggtccatg gcagcagtgc
     9961 cagattcagc atgcccaccc ggactgcggc cagcgcaatg ccgatgcctg cagtggctgt
    10021 cagtgcgagt aacgccaggc gcatcgcgcg aacggttgcg tgccgtaccg gcgagtgcag
    10081 caggctgtga ccgcaggcca gcagcagcac gccacacgcc agcgtcagtg cggccatggc
    10141 cagcctgatg tcgtgcgcat cggcgcgatc cagtccgcgg gccagccaga gtgtgcctgc
    10201 catcagggga agatgtacca gtcctgccac agtgcgcgga aaagcgatgg gtgcgccggc
    10261 gaccactggc aacatctgca tcagtgcacc ggccatgctg gtggcgagaa accccagtgt
    10321 aagcaggtgg acgagcgcca gcgtggccgg gtgccagcgt gacgccagca gctccgggcc
    10381 gttggccagc atcaggccgc ctgcggccag caaaaagacg ggcgcggcaa gaaagaaacg
    10441 gaacggtacc gaaatcgggg gagcctgttc cagggccagg ctggtgtgcg cgccgttcat
    10501 cgccagatca ggatgtcgac gcggcccgga gcacccgttt cagtgcgata gtgaaatccc
    10561 gattcctcca gcgccgtgta cagcggaaat ggttcgcggt ggtggatcat gtgcaatacc
    10621 tgtccggccg gcaggctgtc cagttcatgc aataccttga gcatcggttc cggaggctcc
    10681 aggccgcgta cgtcgatgag gcgttcatcg gccatgggat cagcctgccg actggagttg
    10741 gcgcatttgc agcaccatcc catcgtgctg cccgccgaaa agtcggtcgg tcatcggata
    10801 cagcacctgt tcttccttga tattgtgctg ctggatgaca atgagcaagg tttcggccag
    10861 gccgagactg ccttctacat cgtgaatgac gatggcattg gacacacggt cgaacatgcc
    10921 gcggatttcc tcgtgctcga tgcgcatcat gcgtgtcggc cccgaactgt tgccggtggc
    10981 ctgttcgaat gcaggaaaca ccaccgtctc ttccttctcg aaatggttga gcgtggccgc
    11041 agtgaaacgc tggaaccagg tttcggcctg catccagtcg ccatcggcaa tggcctgctc
    11101 ggcgtaggaa aacagtacat cggcttcgcg atgctcggcg gtgaggtagc tgctgatcgt
    11161 gctcatgatc cgttcctgaa aatgaagtgg ctggaacgga tattgtggcc aatcccgcac
    11221 tgcgttgcct tgacctgagt caaggcaacg cagtgcggcg gcgcggggcg tacgttggcg
    11281 gtacgcccgt tacgccgccc gagcgggcga tcagtcgctg aacgggtgtt gcagaacgat
    11341 ggtttcctcg cggtccggcc cggtggagat cagggcgatg gggacttcgc acagctgctc
    11401 catgcgctgg agatacgcct gggcgttggg aggcaggtct tcgaagtgtt tgacgccgac
    11461 cgtgctggaa ctccagcccg gcatttcttc gtagatcggt tcgcaggttt cgaggttctc
    11521 cgcgccgacg ggcagaatgt cggagtcgcc ctgttccatg tggtagccaa cgcagatgcg
    11581 cagcgattcc atgccgtcga gcacgtccag tttggtcacg cacagaccgg acacgccgtt
    11641 gagctggatc gaacgtttga gcgcggcagc gtcaaaccag ccgcagcggc gcgcccggcc
    11701 ggtggtcgag ccgaattcgt gcccgcgttc ggccagttgc ctgccggtgt cgtcgaacag
    11761 ttcggtgggg aacgggccgg agccgacgcg cgtggtgtac gccttggtga tgccgagtac
    11821 gtagtgcagc gtgtgcggtc ccacgccggc acccgtcgag gcgccaccgg cggtgcagtt
    11881 gcttgaggtg acgtacgggt aggtgccgtg gtcgatgtcg agcagggtac cttgtgcacc
    11941 ttcgaacagc aggtttttgc cgtcacggtt ggcttcgtag agcaggcgcg gcacatcgcc
    12001 caccattggc ttgatgcgtt cggccagcgc gagcgtggtg tcgagcgtgt gattgaaatc
    12061 gacgatgtcg gtgtggaaat agtgcttgag cacgaagttg tgataatcga gcacctcacc
    12121 gagcttggcg gcaaagcgtt cgcggtagaa cacatcctgc aggcgaatgg cgcgccgggc
    12181 gaccttgtct tcataggccg ggccgatgcc gcggccggtc gtgccgatct tgccagcgcc
    12241 tttggcctgt tcgcgcgcca gatcgatggc ggcatggtag ggcaggatga gcggacaggc
    12301 ttcggagatc ttcagtcgtg cggccacttc aacgccgtgc gcggcgagca tgtccatttc
    12361 ctcgagcagg gctgtgggcg acacgaccac gccgttgccg atgtaacagg tgacatcgtt
    12421 acgcaggatt ccggaaggaa tgaggtgcag gacggtcttc tgaccgccga cgaccagggt
    12481 gtggccggcg ttgtggccgc cctgaaagcg caccacgccc tgggcgtggt cggtcagcca
    12541 gtctacgatt tttccttttc cttcgtcacc ccactgggtg ccgatgacta cgacgttttt
    12601 gctcatgttg aatcgcttgt ggtgaataac ctgctggcct gtcagtctgc tggccgggca
    12661 acgaccgccc agtcgccttc tgccgttggc tggagtatgc ggtcgtgatc gtattctgcg
    12721 cggctgctct cgtggccggg caagtcgacg atgacggttt cgcctgcaag acgcaattcg
    12781 tcgatgcggg cagacagtct ggcgtttttc aggtaaggcg caagaatcgg gcggttgacc
    12841 tggggccgcg gaaacagggt tgccagttcg cgcaggtcga ggctgaatcc ggtggcgtga
    12901 cgggcgcgcc cgaaggtttt gcccacatga tcgaagcgtc ctccggaagc gagcggtgcc
    12961 gggtggtggc tggtgtagac cgcgaatacc acgccgctgt ggtaatggta gccgcgcagt
    13021 tcggccaggt cgatgttgag cttgatgcct gaccgggttt cgttgacgat ggcgcgcagc
    13081 tgttcgagcg cctggctgat ttcgaaatgg ctgggcagtt cgcggaaggc gcgttcgagc
    13141 acttcttcgc cgccgtacag tgtcggcagg gtgcagaagg cggcacgggt ctgcggttcg
    13201 aggtcgcggg tcagatgccg gatgctggcg atgtccttgg attgcagcgc ggcgaacagc
    13261 gggccggcct gggcctcctc gatgcgcgca tactgcagca gtccgcggaa tactccgacg
    13321 tgtccgagat cgagatgcag gtcggatacg ccagcaagac gcaaggccga cagcatcagt
    13381 ttctggattt cgatatcggc ctcgaagcct tcatgtccat acagttcggc gccgagttgc
    13441 atcagttcgc gcgaacgccc ggtgcgttcc ggcagcgtgt gcaataccgg tcctgcatag
    13501 cataaccggg tgacgccctg ccggttgagc aggtgcgcgt cgatgcgcgc ggtctgcggg
    13561 gtgatgtcgg cgcgcaatcc catggtctgg ccggagatct ggtcgaccag cttgaacgtc
    13621 ttgaggtcga ggtcgccgcc ggtgccggtg agcagcgagt cgaggtattc gatcagcggc
    13681 ggggccacca gctcgtagcc atgcacgcgg aacaggttga gcaggttgtg ccgcaacccc
    13741 tccatgcgca gcgcggcagc gggcaggatg tcctcgatgt attcgggcag ccgccaggtg
    13801 tgcgtgttca aggtttgctc cccccgggtg aattcatgta cttgaagaag gccgagtgcg
    13861 gatcgagtac cagcatgtcc tgcttgctgt ggaagctttg ccggtaggca ttgagactgc
    13921 ggtagaacga atagaactcg ggattctgcg agaaggcgct ggcatagatc gcacttgcgc
    13981 tggcatcgcc ttcgcccttg acgcgctgtg cgtcacgcca ggcatcggca atgatgactt
    14041 cgcgctggcg gtcggcattg gcccggatct gctcggcctc ggcggaaccc gtggagcgca
    14101 gttcgttggc cacccgtttg cgttcggctt ccatgcggcg gtagaccgaa tcactgactt
    14161 cttgcggcag atcgacatgc ttgatgcgca cgtcgatgac ttccacgccg attttgcgcg
    14221 cgtcctggtc ggcgcgggcg cgcaaggtgc gcatgatgtt ctcgcgttcg ccggatacca
    14281 cgtcatggat ggtgcgctgg ccgatctcgg cgcgcagggc atcgttgatc gtctgctgca
    14341 accgggtgcg cgctcgtgtt tcgtcgccac cgacgctgac gtagtactgg cgcacatcga
    14401 cgatccgcca tttggcgaaa tagtcgatga gaacgttctt cttttccgag gtgatgaagc
    14461 ggtccgggtc gttcggatcc agtgtgagga tacgtgtatc aaagtaacgt acattctgca
    14521 ccagcggcat cttgaaatac aggcctggcg cgcgctgggt ggccacgatc tgtcccagct
    14581 ggaacaccag cgcgttctgt cgctggtcga cgctgtagag cgacaggctg gccaggatga
    14641 gcacgcctgc aatcagggtg atcagcgaag caatccggtt gttcatggcc ggtcctcccg
    14701 gtcgcggctg cgcatggcat cccgcgtgcg gttcatgtcg tcgggagacg acgagtcatc
    14761 gggtcgtggc gtgctgtcgg ccgtggtgtc tgccggtttt ggggtgtcgg ctgcacccgg
    14821 tagcggcgcg gtcgctgcag gtgggggtga cgcgagcggc gcaacggcgc tgccggcggg
    14881 cgccgtctgc tgcagcaatt tgtcgagtgg caggtagagc atctggttgc tgcccttgcc
    14941 gctgtcgacg agtaccttgg tggtgtgggt gagcacctgc tgcatcatgt cgaggtacat
    15001 gcgttcgcgt gtcacgaccg gtgccttgtt gtactgggcg agcacctggc ggaagcggtc
    15061 ggcctcaccc tgggcatcgg cgatcaccga ctgcttgtag ccggcagctt cttcctgcag
    15121 gcgggctgcc accccgcggg ctttgggtat cacgtcgttg gcgtaggcct gtccttcgct
    15181 tttgagccgg tcacggtcct ggccggcctt gacggcatcg tcaaacgacg gctgcacttc
    15241 ctggggcggt tgggcgttct gcagggtgac gcggctgatg ctgatgccgg caccgtagcg
    15301 gtcgagaatt tcctggatgt gcttggcggc acgttcggcg atatcggcac ggccttcata
    15361 gaggacatag tccatctggc tcttgccgac aatctggctc atgacgcttt cggcgacctg
    15421 gcgcacggca tcatccggtg agcggttgtt gaacagatag tgttccgggt tcttgagcgt
    15481 gtattgcacg gcgaactgga tgtcgacgat gttctcgtcg tcggtgagca tcagcgattc
    15541 cttgagaatc ttgttgcgca cattttcgcg gtaaccgact tccaccgtac gcacctgttc
    15601 catgttgacg atttcgaccg attcgacagg ccagggcagg tgccagccgg gtccgggctg
    15661 gctggtttcg atatagcggc cgaaacgcag caccacgccg cgctcgccgg catcgacgat
    15721 gtagaatccg gaggcgagcc agatggccag caccacgccg gtgacgatga ggaacaacga
    15781 tggcggtacg ccacggcccg gctcggtctt tctgccgaac agcttgttca gccgtgcatt
    15841 gaagttgcgc cagatttcgt ccaggtcggg cgggccatcg tttttcttgc cccactgcgg
    15901 gtcattccat gccatgctca gtccagttcc ggatggtatt cgctgttggt cgttcgggcg
    15961 ttttcacttg tgctgccgga gcgcaggaag tcttgcctgg cctcgccgag cgccgtgcgc
    16021 agcagagaaa taccttcgcc ggtctgcgcg cttagccgga tggcgctgat tctaccatat
    16081 tcgtcgcgtt cgatgcccgg gctgcccgga ccgaggtcga ttttgttgta tatcagaatt
    16141 tgcggaatgg cgtcggcgcc gatttctccg agcacgtggt tgacttcggc gatgtgccgg
    16201 tcgcgcatcg ggctgacgct gtcgacgaca tgcagcagca ggtcggcctc gcgggtttct
    16261 tccagtgtgg cgtggaagct ttccaccagt gtatgcggca ggccggtgat gaagccgacg
    16321 gtgtccgaga gcacgatttc gccggtttcc ggtaaaaaca gctgacgcgt ggtggtatcc
    16381 agcgtggcga acaactggtt ggcgacgtag ctgttggcat gggtcaggcg attgaacagc
    16441 gttgatttgc cggcattggt gtagccgacc agcgataccg acatgacctg gcgacggcta
    16501 cgcgcgcggc gctgcacctt gtgctggcgc tgcacgcgcg ccagacggtc cctgagcagc
    16561 ttgacgcgct tgccgatcag tcgtcggtcg gtttcgagct gggtctcgcc cggtccgcgc
    16621 aggccgatac cgcctttctg ccgttcgagg tggctccagc cgcgcacaag gcgggtggcg
    16681 aggtgggcga gctgtgcgag ttcgacctgc aggatgccct cggcgctctt ggcgcgtcgc
    16741 gcaaagatgt cgaggatcag cgtagtgcgg tcgaccacgc ggcactccag agcgcgttcg
    16801 aggttgcgtt cctgtgccgg cgacaacgga tgattgaaga tgaccaggtc ggcctcgtgc
    16861 gcatgcacgg ccgcgccgat ttcctcgacc ttgccgctgc cggcgaagta ggtggggtca
    16921 ggtcgcgcgc gccggccctc caccacgccg gcaacggtga ggccggcgct ggttgcgagc
    16981 tggtgcagtt cttccagact ttcccgatag tcgggctggc cggtgtcgat gctggccagc
    17041 accacccggt cgcccccctg gtggcgttca aacatccgcg tatcctggag gcaggcggcg
    17101 tgcccctcgg gcgggaagac tgcagagcat gcggttcatg gcgaagcggt gcagggcgtt
    17161 ctcagccgat gccgttttcc tggttgtgca atacaacggg acgcgccggt acgatggtgg
    17221 aaatggcgtg cttgtatacc atctgggtga ccatattctt cagcaggacg acatactggt
    17281 cataggactc gatctggccc tgcaatttga tgccgttgac cagatagacc gagacgggca
    17341 cgttctcccg gcgcaaggtg ttcaggaatg ggtcttgcaa ggaaagtccc ttgtttttat
    17401 ttggttttat tgcgttcatc gtggggcagt ccaatcgtta tgacgaaaca gcgtcgggta
    17461 attatatacc ggggctgacg gcagttggca ataaccgcgt cgccggcgcc aatgtgtgtt
    17521 gattgacagc gggtttgcct ccaggttccc cggaagctgc gtgcaagtct catatgcatg
    17581 ccatttgagt gttacggcgt tttgtgtcag catgggcacc actgtcgcaa ccgcgcgccg
    17641 cccggatcgg cccgcgccgg tgcgagtttc gtccggagaa aaggcatgac agattgcacc
    17701 cgtcacaagg cgtggggagc gctgcaggcg cacagtcggc agatgcgaga cgtgcagatg
    17761 cgcgacctgt ttgcgcaaga cgagcagcgc ttcgagcagt tttcgctgcg agctgcgggt
    17821 attctgctgg actattccaa gaatcgcatc actgccgaga ccctgtcgct gctgaccggc
    17881 ctggcacggg aagccggact gggcgaggcc atgcaggcca tgtttggtgg cgaccgcatc
    17941 aatgttaccg aaggtcgcgc agtgctgcac acggcgctgc gcaaccgtgg cgactgggcg
    18001 gtgcaggccg atggcaagcc ggtgatgccg gacgtgctgc tgg
//
LOCUS       DAIANL010000016         8118 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_829587, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000016 DAIANL010000000
VERSION     DAIANL010000016.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829587"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1633 a         2645 c         2356 g         1484 t
ORIGIN      
        1 cagcatttca ctggattacc atgaaaccct gcaggcggcc tccggtacgg ctgccgaggc
       61 cgcacgcgag caggcgatga aagcgtttta cggcaaatac ctgctgatcg tcgacggttc
      121 ggtggccacc ggattgcaag gcgcctattc gaccgtggcc ggcgtcacca atctggacga
      181 actgaaaaaa gtggcggccg gcgccgccgc ggtggtcgcg gtcggttcgt gttcgtcgtt
      241 cggtggcctg ccgatggctc accccaaccc gaccggtgcg gtggcggtat ccgacatcat
      301 caccgacaag cccgtgatca acgtgccggg ttgcccaccc attcctgtgg tcatcaccgg
      361 cgtgctggca cactttctca cctatggcac gctgccggaa accgacctgc accggcggcc
      421 gaaaatgttc tacggtgaga ccattcatga tcgctgctac cggcgaccgt tctacgatca
      481 gggcaagttc gcccacacct tcgacgacga aggtgcacgc aacggctggt gtctgtacga
      541 actcggctgc aaggggccga tcacctacaa cagctgtgcc acgctgaaat ggaatgaagg
      601 caccagcttc cccatcgaat ccgggcatgg ttgtctgggg tgttcggagc cgaagttctg
      661 ggacaagggc agtttctacc agccgctgtc cggtggcacc tggactgaca gcggcacgct
      721 gctggcggcg gcagcagccg gagtcgctgt cggcgccggt gcggcagtac tgtcgcgcgc
      781 ccgccagcat cacgcgcaag aggagaaatg aatggacatg ctcgcctttg cccgcggacc
      841 ggggctgcac tggtcgctca ccatcctgtt cgtcggcgtg ttctggcgcc tgaccggtat
      901 cctgctgctg cgccgcagtc cggactacag tgtcgcgcgc atctccggct ggtggacggg
      961 cgcgctcagg ctgatcgcgc tgcgctcgtg gccaagacgc gaattctcgg ccacgaccag
     1021 tttccccacg ctgatcggct acctgttcca catcggactg gcagtgatcg tgttcggcct
     1081 gcagcaacac attctgctca tccgctcgct gaccggcctg tcgtggccga atctgcccag
     1141 cggcctcgtc aatctgaccg ccggcgtcac ggcggcatcg atgctggcgc tgctgatccg
     1201 ccgcatgacc aatccggtgc tgcggttact gtccaatttt gacgattatt tcagctggct
     1261 ggtggccttt ctgcccgtcg ccaccggcat gctggcggta gcgcatctcg gcgcgcgcta
     1321 cgagaccttg ctggcgctgc acatgctgtc tgcggaactg ctgtttgtct ggttcccgtt
     1381 cggcaagctc atgcatgcca gtctgatctt cctgtcgcgc ggcacctcgg gtgccctgct
     1441 gacccgcaaa ggagccgcga tatgaatacc gtggaaatcg atgcagttca tccgctgatg
     1501 acagaaggcc cgcgtctgac cgtcgaaccg cccgcaaccc gttatgacaa gcagggtgat
     1561 ctgcccgacg gtgaacgcgc cgcacgtgcc gtacgccagt tcgtgcgcga tttcggcgcc
     1621 acggcggcca cctggctgga atcctgcatt cactgcggtc agtgcgccga agcctgtcat
     1681 tactacgtcc agaccggcga agccaaatac acccccgtgt ggaaactcga accgttcaag
     1741 caagcctaca agcgtgaagc cggtccgttt gcgccactgt accgggcgct gaacctcaag
     1801 cgcgacgtca gtgccgcaga actcgaatcc tggcaggaac tcatttacga ctcgtgcacg
     1861 ctgtgcggcc gctgcagcct gatctgcccg atgggcatcg atctggccag cctgatttcc
     1921 caggcgcgtc acggcatggc gaaggccggg ctggtgccgc acgagctgtg ggcggtggcc
     1981 gagcgcgcca gcgtcgaggg cagtccgctc ggtgccacgc ccaaggtctt ccgcgaacgt
     2041 tgcgaatggc ttgccgacga acacaacgtg gcgatccacg tcgatctgcc cgaagccgac
     2101 atcctggtga gcatgtcatc gatcgagatc atgaaatatc cggattcgat cgtggccacg
     2161 gcgcgcatcc tcaattatct gggaacgagc tggacattcc gcaccgacgg ttatgaagcc
     2221 accaatttcg gcatgctgtc gggcaacgcc gactggcaga gggaaagcag cctgcggctg
     2281 atcaatgcag ccgttgcctg caaggcgaag cgactcatcc tgcccgaatg cgggcatgcc
     2341 tatggtgcat tgcgctggca gggcgccaac gtctatggtc agccgctgcc gttcgaagtg
     2401 gaacacatct ccgaatttct tgcccgcgaa atgcaggcag gccacctgcc cgtcaaaccg
     2461 atgccggcca gcgtgacttt tcacgacccg tgccagatca gccggcgcgg gggcgcgaca
     2521 caggcgccgc gtgtcgtgct cgacaagctc ggcgtcgacc tgcacgaaat ggagatgcac
     2581 ggcaacctga actggtgttg cggcggcggt ggcggcgtgg tgaccatcca tcgcgccgat
     2641 gccctgcgtt acaaggtatt ccagctcaag atgcggcagg tggaagacac tggcgcgtcc
     2701 atgctggtca gcagctgctc gaactgccgt cagaccttcg atgacggcca ggcacatttc
     2761 cactgggaca aaaccatgca cagcctgctc gaactggtgg ctgaccacct gatcgaaccg
     2821 gccgccagca aggagaatgc agcatgaaca cccgtaccgt cgtggtcgac ccgatcaccc
     2881 gcatcgaagg ccacctgcgc atcgaggccg aaaccagcga ttcgggcgag atcctgcgcg
     2941 catcgagcgc cggcaccatg gtgcgcggca tcgagatcat tctgcgtggc cgtgacccac
     3001 gcgatgcctg ggccttcgcc cagcgcatct gtggtgtgtg cacactggtc catggcatcg
     3061 cctcggtgcg ggcggtggaa aacgcgctga acatcgatat tcccgccaat gcacagatca
     3121 tccgcaacct gatgattgcc gcccagcatg tgcatgacca tgtgatgcat ttttaccatc
     3181 tgcacgcgct ggactgggtg gatgtggtct cggcgctcaa ggcagacccc agggccacct
     3241 cgcaactggc gcagtcgatc agcaattacg ccaaatcgtc gccgggttat ttcaccgaca
     3301 tgcagaacaa gctgcgcggg tttgtcgaat ccggccagtt gggcatcttt gccaatggct
     3361 actggggcca cccggcctac aagctgccac ccgaagccaa cctgatggcg gtggcgcact
     3421 acctcgaagc cctggcctgg caacgcgacg tggcccgcct gcatgcggta ttcggtggca
     3481 agaacccgca tccgaacttt ctggtcggcg gcgtgccctg tgccatcagc gtgcatgccg
     3541 gcgagaacac tgccgatggc gccgtcagcg gcgcaagcgc gaccgcggtc aacgacgacg
     3601 ggctggcgct ggtcaagacg atcatcgacc gcatgcgcga attcgtcgac caggtgtacg
     3661 tacccgacac gctggccatc gccggtttct acaaggactg gttcaaacag ggcgaaggcg
     3721 tgggcaattt catgacctat ggcgattttc ccgcgcacgg catgagcgat ccggccagtt
     3781 tcctgattcc gtcaggcgtc atcctcaacc gcgacctgac agtcattcac ccggtcaatc
     3841 tcgaagccga tgaccagatc caggaatttg tcgcccactc ctggtacgac tactccggcg
     3901 gcaaacatca gggactgcat ccgtgggcgg gcgaaaccaa cctgcactat accgggccga
     3961 ccccgcccta cgaacatctc gataccgaac agtcgtattc gtggctcaaa tcgccacgct
     4021 ggcaaggcaa ggcagtggaa gtgggtccgc ttgcgcgcgt gctcatgctc tacgcacgcg
     4081 gccacgaaca gacccgcgcg ctggtcggca tgacgctcga aaaactcgac gtgccgatcc
     4141 aggcgctgtt ctccacgctg ggccggactg cggcacgcac cctggaaacc aaggtgatcg
     4201 tcgacaacat gcagcactgg tatgacgagc tcatcgccaa catcaaacgg ggcgatgtgc
     4261 gcaccttcaa cgaaacgcgc tgggatcctt ccacctggcc tgccaccgcc aaaggcgtcg
     4321 gattcatgga agcgcctcgc ggcgcgctgg cgcactggat cgtcatcaag gacggactga
     4381 tcgacaacta ccaggctgtg gtgccctcga catggaacgc cggcccacgc gacgtgcagg
     4441 gccagtcggg accgtatgag gccgcgctca agggacacag cctgtatgac gcaaaacagc
     4501 cgatcgagat cctgcgcacc attcacagtt tcgatccatg cattgcctgc gcggtgcatg
     4561 tggtcgatcc ggccggggaa gaactgatcc aggtcaaggt gcgctgaaca gagggttgca
     4621 gcgtgctgcc catgcaggta gcggcacgcg ttttgccatg cacttggtgt atgatatccg
     4681 ggttttcaac cgaggatgca tcatggccgg tctgacccgc gatacaccga ctcccaccga
     4741 tatcacgctg caccagcagt cgcgcgtgct cgaaatcagt ttcagcgacg gccatacttt
     4801 ccggcttccg tacgaattgc tgcgcgtgta ttcgccgtcc gcagaagtgc gcggccacgg
     4861 tcccggacag gaagtgctgc agctcaacaa gcaggatgtc accatcaaca acatcgaacc
     4921 ggtcggcacc tacgccatcc agccggaatt ttccgatggc cacaacagtg gcatctattc
     4981 ctgggactac ctgcaccagc tcggcctgca ccagcaggag ttgtggaccg agtatcagca
     5041 gcgcgtccag gccacccagg caagcacttc cggcaaaggt cactgaaccg gcacatgcag
     5101 cagttgttgc tcgacatcca gccgcacgcc gcacaggatt tcgcgcattt cgtacccggt
     5161 aatggcaacg aagccctcga tgcgctgcgc acgctggtac agcaccgcgg cggcgaaccg
     5221 ctgatctacc tgtggggagc gcccggcagc ggcaaaagtc atctgctgcg cgcctgtgtc
     5281 gaactggcac gcgaacaggg cgacagcgcg caactgctct ccgctggcga cgccctcgcg
     5341 ccagaacacc cggcgtggtt caccgcgatc gatgacgtgc acactctgga cgccgacagc
     5401 cagatccggc tgttccagcg catcaaccag gcccgcgaag ggcacggccg actggtgtgc
     5461 gcaggccctg tcgccccccg gctgttgccg ctgcgcgccg acctcacgac ccgactgggt
     5521 tggcacctgg tctatcagtt gcaccccctg gccgactccg acaaggtcgc ggcactgacc
     5581 ggctgggcgc ttgcacgtgg cttccgcctg tctggcgaac tggtggacta cctgatgcgc
     5641 cactggcggc gcgacctgcc ttcactgatc cagattctcg aacagctcga ccgccattcg
     5701 ctgcaggaca aacggcccgt cacgctgcca ttgctgcgcg agattcttgc cagccagtca
     5761 cggccgcagt aatgcaacgg gctgctcacg cggcaaatag ccatcggtat ccgaacgcca
     5821 gtgcgtcagg gcatcgcatc gggaacgcgg taacggagcc taaattacgc tttgattcaa
     5881 tttgttaata ccattttccg ggcaattgtt tcctgacgtt caggcgtgcg ttttccaggt
     5941 tgtctgcaca ggcaattgat cccggagtca tgcgaatgcc tgtcgatgta ccagccaata
     6001 ttgtcatggc ggtatgaacc acgtcacatt tcagcggcct ccgcgcctgg cagcgccgtt
     6061 ccgccattca acgaatactt gccacatgat cccccataaa agaccagggg catccgaaga
     6121 tgcccctggt gattactgct gcacgatcat ctggcgcctg ctccgcaggc gccccgttgc
     6181 cgcaacaggc ggattacata ttgtaagcac gctcgccgtg gctggtgatg tccagacctt
     6241 cgcgttcctc ttcttccgga acacgcagcc ccatgaccat gtcgatcagc ttgaacagca
     6301 cgtagctgcc tacgccggac cagacgatga ccacgccggc atcccacaac tggctgatca
     6361 tctgtgcggt cagatcgaag tcggccacct tgttggcgcc gtaatcgaac acgcccgaac
     6421 cgcccagcgc cgggttcacc aggataccgg tggccagcgc gccgatgaag ccgcccacgg
     6481 catgcacgcc gaaggcatcg agcgagtcgt cataaccgaa catgttcttc atggtgttga
     6541 cgctccagaa acagaccacc gacaccacga cacccagcaa gagcgatccc atcggaccgg
     6601 cataacccga agccggcgtg accgccacca gaccagccac cgcacccgaa caggcaccca
     6661 gcaggctggg cttgcccttg agcagccatt cgatgcccat ccacgtcagc gcggctgcag
     6721 ccggagcgac gatggtgttg gtcatggcca gcacggcagt cagggctgct tccaggttgg
     6781 aaccggcgtt aaagccgaac cagcccaccc acagcatcga agcgccgatc atggtcatca
     6841 ccatgctgtg cggtgccatc gattccttgc cataaccgat gcgcttgccg agcaccagag
     6901 cacccatcag cgcagccata ccggagttga tgtgcaccac agtgccgccg gcaaagtcga
     6961 gcgcgccctt gttgaacagg aagcctgccg gaccctggta gttggacgga ccgctccaga
     7021 accacaccat atgcgcaatc ggcacgtaga cgaaggtgaa ccagagcacc gagaacacca
     7081 gcagggaaga gaacttcacc cgctcggcca ggccaccgac caccagcgca accgtgatcg
     7141 cggcaaaagt cagctggaac accatgtaca ccatttcagg gatgtacacg cctttggtga
     7201 aggtttcgga catcgcactc ggatccatga tgcctttgag gaacaggcgt gacgtgccac
     7261 cgatccagtt gttgagcgac ccgccgtcag tgaaggcgaa cgaatagcca tagaccatcc
     7321 acagcaccga aatcagacag aaaatcacga acacctgggt cagcatcgac agcatgttct
     7381 tggtgcgcac caggccaccg tagaacagcg ccagaccggg aatggtcatg gcgatcacca
     7441 gcaccgtgga aatcagcatc caggtggtat cgcccttgtc agccaccggc accttggtgg
     7501 ccgcagctgc agccgcggca ggtgccgcaa ctgccggagc cgccgcaggt gcggcaacag
     7561 cgggcgccgg ggctgccgca gtggccgcag cggctggtgc agcagccgga ggcgccgcag
     7621 tgtcatccgc catactcacc ccatgcaccc caagggtaaa catcatgctt aacgcaacga
     7681 gccatttttt cattttgctc tccactgtta taaggcttct ggtccggatt cgcccgtacg
     7741 tatccgcacg ctctcttcca gattgaacac gaagatcttg ccatcaccga tcttgccggt
     7801 atgcgccgac ttttcgatgg cttcgatgac ctgatcgagc agttcgtcgg caatggccgc
     7861 ctcgatcttg accttcggta aaaaatccac cacatactcg gcgccgcgat acagctcggt
     7921 atgccccttt tgacgtccaa accctttgac ctcagtcacc gtgatgccct gtacgccaat
     7981 ggcggacaat gcttcccgga cttcatcgag cttgaacggc ttgacgatcg ccgtaacaaa
     8041 tttcatgtca tctccttgaa aggtatggag tttgcgccac tcagaacatg tacattgcct
     8101 ggaattccac ggacgtct
//
LOCUS       DAIANL010000017         3952 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2260887, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000017 DAIANL010000000
VERSION     DAIANL010000017.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2260887"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          766 a         1068 c         1309 g          809 t
ORIGIN      
        1 ccccagcgct gccagtactg tttgccggta gctggccagc gcctgacgat acccggctga
       61 ggcggctgcc tgtttggccc gaagcgttcc gccatcgaac accggcaggc tgacatcagg
      121 accgaaactc cagaacgtgg cattgctctg tagcagatta ccgaatgaag tgttgttctg
      181 gccatagcct gcatcaagtg tgaatgccgg atacagcgcc gcggtggcca cgccgatggc
      241 agcactggcg gcatgcaggc tggcctcggc ggccacgata tccgggcgct ggcgcaccag
      301 cacggagggc aacgtgaccg ggatctcctg cggcagggtc agctcgtcca gacggattgt
      361 cggtggggtc cattgcgcgg gcgcgtggcc tgcaagggtg gcgagcaggt ggcgggactg
      421 ttcaagtttc tgccgcagcg gtggcagcgt ggcctcgctt gcggcgagcg cggatttcag
      481 cgccagcacg ctggtataag gaacggtgcc ggcctgtgcc tgagtccggg taagcgcaag
      541 ttgtcgttgt tgcagcgtga tgacctgttg cgtgatatcg atttcatcga ggtaagccgc
      601 ctgggcaatg gcggcattga cgaggttgcc ggacaaggtc agataggtgg cctcgacggt
      661 ggcccgctga aatgcggtct gtgcctgcag accctcgatg gtacggcgct gaccgccgaa
      721 gacgtcgagc gcgtaactta tcgaggctga cagtgtcagc agattgaaca gactgggcgg
      781 tgcattgctg ccgaaacggg ccggagaaaa tgcctgccgg ctggcattga aatctgcatt
      841 cacctgggga tagaacacgc catagccggc gcgcaggttg tcctggctct gttgcagact
      901 ggccagctcg gcctgcacac cgggattccc ggcaaatccc gcggccacca gtgcgtccag
      961 ccgtggcgag ccgaagcgct tccaccagtc ggccgccggc tgcgacgatg gtgtgaatcg
     1021 ctgggcggtg ccgccggcgg atacggtagt ggtgggcagt ggcgtgcgag tgtagcgatc
     1081 ggtctgtggt ggttggggac ggacaaaatc ggggccgacc gcgcatccgg acagcaaccc
     1141 ggccgcgagt accgacaggc acaacgccgt cgcgcgagcg tatttggatc gagacacatg
     1201 aggtaatgca gcagcgagac gcatggattt cctctactat cgggttccaa tatagacatc
     1261 gaccagctgg ccggggtaga tcgctgggtg tgtcggcttc ctgaaacgga aaatcaccgg
     1321 cagtacacgc acatcgactc gttcggtcct ctggtcggac aactggatct tgggcgagac
     1381 ataaggctga atgcgtacga attcaagcgg cagactgagg ctggtgccgc gaacgaacat
     1441 tctggcacgc atgctggcag gagccgggag ccggttgaca agaatttcat ccacataaca
     1501 tcgtacgtcg agatagggct gttctgcgcc caacacaatc gccgggccat aaccttgcgt
     1561 gtaggtgtca tagacgcctt gtgtcgatga gacataactg ccgatagcgg tattgatggc
     1621 cagaattttg ccgtcgcgcg gggcgcgcag agtgtatttg gccagaaggg cctgggccgc
     1681 gcgccgggct ttttcctgcg ccacggcctg cgttttggcg acgtcaaggg ccgagcgtgc
     1741 gatcagcatg gcatttctgg cgttgtcgag cgcatcgctg ctgacggatt ccgggtcgag
     1801 ctgccaggaa tggcgctgtt tgtcgagctg gtcctgtgca tttttcaggc tggccttggc
     1861 ctgagcgatt tgcgcacgcg ctgcctgcgt ttgggcggcg atctgttcgg tggtggcgcg
     1921 ctggatggaa tcgtcgagta gcagcaccgg gtcgccgctg cgcacctgtt gcccctcggt
     1981 cacgggaatg ccggtcaccg tcgccgagac ttcaggatag aggttgatgt tggcgccact
     2041 gatctgatcg ctttcgatga taccgttagc gtaaatgcca tcgctgtacg ggttggcggc
     2101 ggggatgaat gcgggcggca agggttgctt gtgcagtccg gaaaaatggg ctgcggtcaa
     2161 cccggccagc acgccgagca cggccaggag gaacaacagt ctagttttca tggggatcct
     2221 ctcggttgat gccaatgatc ctgccgtctt ccatgttcat gatgcggtcg gcatattcga
     2281 aaatccggtt gtcatgggtg acgatgatga tgcaacggtg gtcattcagg atgttgtgtc
     2341 tgatgaattc aacgatgcgg cgcccggttt cgccgtccag tgaggcagtc ggttcgtcga
     2401 gcacaagaat gtcgggttcg ctggcgattg cgcgtgcgat ggcgacccgc tgctgttcgc
     2461 cgccgctcag ttttaccggt ggcagttgcg cgcgttcctt caggcccacg atttccagat
     2521 aacgcgtggc aacggcaagc gcctgcgacc attccatttt cttcagaatc agcgggatgg
     2581 cgacgttttc ggcggttgtc aggcgaggga acaggtgata gtcctgaaac acgaagccga
     2641 ttgtgttcag gcggaaactg gcaatatcgt cggccgacag tgaccagatg ttctttccgt
     2701 cgacatcgac cgcgcccccg tccggcctga ggatgccgga aatcatgctc agcagcgtgg
     2761 tcttgccgct gcctgatggg ccgacaatgt agaggatctc gccaaaaaaa gcatcgaaac
     2821 tggcgtcgcg cacggcatag gtgcgggcgt cgcctgcgcc gaaccatttg accagggcag
     2881 ttgccctgac tgccagtctg cccatgtcag ctcctgaaga tgtcgaacgg ttcgatgcgg
     2941 atgaccttgc gcacgccgat gtagctcgaa atggcggcga tgagcaccac cattacgaat
     3001 gccaggccaa tgttggtgta cgtgatcatg gcggcatagt cgggcaggcg cagcctggcc
     3061 agggtgatga tcagggctgc cagaccgacg cctaggccga agccggtcat ggcagtcagc
     3121 gtggcctgga acaggatcat cagcaccagt tcgtgtcctt tggcgccgat tgccttgagg
     3181 gcgccaaatt tttccaggtt ctccagaatg aacgtgtaaa acgtctggcc tgaaatcgac
     3241 aggcccacaa tgaagctgat tactgtcatc agcagcacgt tgatgcccat gcccgtctgg
     3301 tatttgtaga atgtcgagac cttgtcgatg aattcgtttt ccgtgagtgc ctggtaaccg
     3361 agggaggcca cctgctgttt gatgcgtgca atggcgccag agtttttcgg ctgtaccagg
     3421 atgtaggaaa tggtgaagcg tgtggacgga atgtaggtga tggcgcgtgt gtaggtggta
     3481 tagagggtcg gtacgccgaa cagtccggca gtgctgaccc gggcaatgcc cgtgaccacg
     3541 cccaggtgat cgttgagttc gaaccgggtg ccgatcgtcg ggttgccgag cttgacgaat
     3601 tccgcgtcat cgacaacgat aaaagcattt tctgcaaaga tatcgtcgat tttccccgag
     3661 acgagttccg ggcggccata cagactggtg tcgtcaagac cgatcaccga gactgcctga
     3721 taggtgccgt tgcgcaactt gacctgtgcg ccacccgaat agagtggcac cgcgtattgc
     3781 acgccgggca ggctgcgcac cgcatcgagc acgtagtcgg gcatggggat gctgctgacc
     3841 ggagtgttga cggccggatc cagcacccag accggagcac cgacattcag gatggtggcc
     3901 gaggagcgga tgaggatgcc agcaaacatg gaggtcatca tgaccatgag ga
//
LOCUS       DAIANL010000018         4475 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_626875, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000018 DAIANL010000000
VERSION     DAIANL010000018.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626875"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          746 a         1314 c         1537 g          878 t
ORIGIN      
        1 tttcggatgc ggcacggcca catggcctgt actatgcgcc ggacccatca tcgcagatcg
       61 cctgcaccat tggcggcaac gtggcagaga actccggcgg cgtgcattgc ctgaaatacg
      121 ggttgacggt gcacaacgtg atgcgcgtgc aactggttac gatggatggc gactgtctgg
      181 agctcgggac ggaaacgctc gacagccccg gatatgatct gttggcactg gctgtgggtt
      241 ccgaagggct gctgggtgtg gtcaccgaag tcaccttgcg gttgctgccg cggccggagt
      301 gcagccagtt gctgctcgcc gcgttcgatg atgtcgcccg tgctgccgat gcggtgggcg
      361 ccatcatcgc ggccggggtc attccggccg ggctggagat gatggacaat ccggccattc
      421 gtgccgcgga agcgtttgtg catgcaggct atccgaccga tgcggcggcg ttgttgctgt
      481 gcgagctgga tggcccggtc gcggatgtga tcgagcagac cggatgggtg gaagccgtgc
      541 tgggcaaagc cggtgcgagc gaaatccgcc gtgctcgcga tgaaaccgaa cgtgcgctgc
      601 tgtggaaagg gcgcaagtcg gcgtttcctg ccgtcgggcg cctggcaccg gattattatt
      661 gcatggatgg cagtattccg cgccgcaggc tggcggacgt gttgcagggc attgccggat
      721 tgtcgcaacg ttatgggctc gcggtagcca atgtgttcca tgccggagac ggtaacctgc
      781 atccgctgat tctctacgat gccaaccagc cagatgagct ggcgcgcgcc gaagcgttcg
      841 gcgccgatat tctcggcctg tgcgtgcagg tgggtggcag cattactggc gagcacggtg
      901 tcggcgtgga aaagatcgac caaatgtgct tgcagttcgg cgcggccgaa ctggcgtgct
      961 ttcatgccat caaacaggca tttgaccccg atctgctgct caatcctggc aaggctgtac
     1021 cgactttgca ccgttgcgcc gaactgggga cgatgcatgt gcatgcgggc cagttgccgc
     1081 atgccggact ggagcgtttc tgatgccccg gcttgtcgat ccggaacagg acagcgacct
     1141 tggcgacagg ctgtgcgacc gcgttgccga ggcgttcgcg caacgcacac cgctgcgcat
     1201 cgtaggcggt gacaccaagg ccgaacttgg tcctgcggtg tgtggcgaac ccttgcgggt
     1261 gacaggtcat cgcggcatcg ttgattacca gccgggcgaa ctggtgctga cggcccgggc
     1321 tggcacgcca ctggcgcaga tcgaagccga actggcgcga catggccaga tgctgccgtt
     1381 cgagccacct cacctggggc cgggcgccac gcttggcggc accatcgcat gcaacctgtc
     1441 ggggcctcgc cgaccctggg caggatcggc acgcgatcat gtgctcggct gcagcctgat
     1501 cgatgggcgc ggccagagga tgcggtttgg cgggcaggtg atgaaaaacg tcgccggata
     1561 tgacgtcagc cggttgatgg cgggggccag tggcacgctc ggcgtgctgc tggacatcag
     1621 cctgaaagtg ttgccgcgcc cggcgacaga agtgacgctc gcactggcca tcgatgcgca
     1681 tgccgcactg gaacgcatgc aacgtctggc ggcgcagccg gtgccgctat cggctgcctg
     1741 ctggctggat ggcagactgt acctgcgttt ctccggcgcg ccggctgccg tggcatacca
     1801 tgcgggctgt gtgggtggaa ccgaggtggc tcccgatttc tggcagtcgg tcaatgaacg
     1861 cagccacagc tttttcagcg atgccagacc gttgtggcga ctgtcgctgc cgacgtgcgc
     1921 aatgcagccc gatctggatg gagactggct gatcgactgg ggtggcgcac ggcgctggtt
     1981 gttgagcgat gcaccggggc agtgggtgca tgagcgtgcg cggcggctgg gcggtcacgc
     2041 tttgcgcgag cgcggggcag gtgcggacaa tgaacgcatg gctgcgaccg atccggcgtt
     2101 actggtgttg tcgcgacggc tcaaacaggt gtttgatccg gccgggatcc tgaaccgtgg
     2161 ccggatgtac cgggagttct gatcatgcaa acccatctgg ctcctcgcct gcgtgacact
     2221 gctgaaggcc gcgaagccga aagcattttg cgctcatgcg tgcactgcgg tttctgtacc
     2281 gcgacctgtc cgacgtacca gttgaccggc gacgagctgg atgggccacg cgggcgcatc
     2341 tatctcatca agcaatggct ggaacaacca caacctgacg ccggaggcgc aaaagccacg
     2401 cgtttgcatc ttgaccgttg cctgacctgc cgttcgtgcg aaacgacctg tccgtcgggg
     2461 gtgcagtatt cccgtctgct ggaaatcggt cggcagcagc tggcgcagga cgtgccgcgc
     2521 cgttggcacg cgcgtctcgg acgccgcgta ctggccggct ggctcgtaca tcgccgcctg
     2581 ttttccatga cgcttgctgc ggcgcggtgg ttgcgtccat tggtgccggc gcatctgcgc
     2641 gccaggcttc ctgctgacgg cgtggctggc gactggcccg cagcgcggca ttcccggcgg
     2701 gtattggtgc tgcagggctg tgtgcaggac agtgctacgc cgggcaccaa tgctgcggca
     2761 gcgcgtgtgc ttgaccacgt cggtatttcg gccattgccg tggcccgggc tggctgttgc
     2821 ggtgcagttg ccttgcatct ggacgatgcc gatggtgcga ggcaggctgc acgcaacaac
     2881 attgacgcct ggtggccggg tgtcgagtcc ggggtggagg cgattgtagc cagcgcaagc
     2941 ggctgtgggc tgatgctcaa ggaatatgat cagttgctcg ccgacgatcc gttgtatgca
     3001 tacaaggcgc agcgggtgtc ggcgctggtg cgcgatctgg ccgaagtgct cgacggtgtt
     3061 gaccttggcg ggctggcacg tgatacgcat gtgcccgttg cagtgcatgt tccgtgcacg
     3121 ttgcagcatg gccagcaatt gcccgatctg ttgccccgtc tgctggaacg cgccggctac
     3181 cggctcactc agacacctga cagtcatttg tgctgcggat ctgccggcac ctattcgctg
     3241 atgcaagcgg atttttccgc gcggctgttg acacgcaagc tcgatgccct gcagtccgga
     3301 cagccggggc gcatcgtcac tgccaatgtc ggctgtcagt tgcatcttgc cagtcaggcg
     3361 cagctccccg tcagccactg gatcgaattg ttcgatccgg acgggacagg tgtgcgccca
     3421 ggttcgggtt gagccatccg tccgctcgat cagcccacag cgagtgacga cgcgtccgac
     3481 gccggcaggt cggtgatccc ggattccacc ccgcgtccga tcaggcgcct gagcagcttg
     3541 tctgcggaga gcacgaccag aatgcgtcgt tcgttgtctc ccggcaacgg tttgacgatg
     3601 ctttccatga gcgaagcgct cttggtgacg aattcggaca tgccttcgat gcggctggcg
     3661 gaaatttcag caatgtctcc caggcgatcg acgaggatgc cgaaccgggc acggtcatcg
     3721 gaagtacgca ggatcagtac ttgcggtttc tgcatgttgt cagtctcgcg atggcgacgt
     3781 tcctgctctg gcctgcgtgt cacgacgact tccgtcaggt cgaaaatggt caccgcccgg
     3841 tccttgtaca tcaggaagcc acgggtccac gcgctggaac ccggcatgag cgtcagctgg
     3901 gcgacgtcga tggcttccac cactgcagtc gatggtagcc cataccagtc gtgaccaatg
     3961 tagaaactgg cgatgtcgac agcatcggca ctgcgggtgc gggccggcgg ctgactggac
     4021 agcggcgctt ccagtccggt cggcaactgg ccgctggcaa tgacccgctc agagagtgga
     4081 gtgaacacca gcccggctac atcggaggcc tttgcactgc cttccgagtc gtattcgcgg
     4141 tatccggcgc gcatgcaggc gccgacggca tagtagcgtt cgcgatacac gaggatctgt
     4201 gagtgacttt ctcccggctg caactcgaga aatctggccg ggatgtccag gtgggtgccg
     4261 gggggcagtt ccgacgcagt gcttgagacg acccggccat cgcgttcggc aaacacggca
     4321 aacgcgccgg ggaacacgct gccgtcggtc tggcgtggca gggaatcgcg taacatggcc
     4381 tggaattgcg gggtggcatc gaacacgatg gcgatgccgc caaccggatc gttgccgtgc
     4441 atatggcgca ccgccgcaga ataaacatag gtgtg
//
LOCUS       DAIANL010000019         6819 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1037273, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000019 DAIANL010000000
VERSION     DAIANL010000019.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037273"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1341 a         2229 c         1872 g         1377 t
ORIGIN      
        1 ctgatccatc accagttcga aagcatccac ccgttctacg acggtaacgg acgcaccggc
       61 cgcatcatca acgtgctcta tctggtcaag gagcgtctgc ttgacatccc ggtgctttac
      121 ctcagccgcc atatcgtgcg caccaaggcg gactattacc gcttgctgca aacggtgcgc
      181 gatgacaatg catgggaaga atgggtgctg tacatgctgg aggcggtgga acaaaccgcc
      241 ggtcatacca ttaccagggt gctggatatc aaggcggcgc tgctcgacta caagcatcgc
      301 atccgcgacg ggttcaagtt ttacagccag gacttgatca acaatctgtt cacccacccc
      361 tataccaaga tcgggttcgt gcagcgcgac ctgaatgtgt cgcgcctgac cgccaccaga
      421 tacatcgatg cactggccga agcaggcttc gtgcagaagc acaagatcaa gggcagcaac
      481 tactatatca acgttgcgct gaaccagatt ttgctgggag cgccccgatg actgaagacc
      541 aaccggagca ggaaacgcca ggttggttga ccgagagtgg ccattcggtc cggacatcgc
      601 gtgcgatggc gacgcgcccg agcgcggcga ttaccggcgg atggagctgg tggagcggtt
      661 gtgcgcggcg gtcggacgat tgaacccggc aataccggcg gtggcgcgcg aggatgcgat
      721 tcagctgata attgacttgg atacgccggt gctgctttgg cgcctatcct acaccatccc
      781 tacacaacac tcaaaaggca aaaaaggcca ttccgaagaa tggcccaatc gcttgacttg
      841 gtggtgggtc tgctcggact tgaaccgagg accaaaggat tatgagtcct ctgctctaac
      901 caactgagct acagacccgt agcgcgtaat tgtagcaccg gagagaccga actgcctgtg
      961 gctttgcccg tgcgctcact gattattgtt gtcgatgaaa ctgcgcagcc ggtcggaacg
     1021 tgaaggatga cgcagcttgc gcagagcctt ggcttcaatc tggcggatgc gctcacgcgt
     1081 cacatcaaac tgcttgccga cttcctccag cgtatggtcg gtattcattt cgataccgaa
     1141 ccgcatacgc agcaccttgg cttcgcgttg ggtaagactg tcgagaattt ccttggtcac
     1201 gtcctgcaga ctgtcgtaaa tggcggcatc catcggtgca agcgtggacg tgtcttcgat
     1261 gaaatcaccc agatgcgaat cctcatcgtc accgatcggg gtctccatcg agatcggttc
     1321 cttggatatt ttgaggatct tgcggatctt ctcttcaggc atttccatct tttccgccag
     1381 caccgaggga tccggttcct gccctgtttc ctgcaggatc tgccgtgcta tgcggttcat
     1441 cttgttgatg gtctcgatca tatgcaccgg gatacggatg gtgcgcgcct ggtcagcaat
     1501 cgaacgcgtg atcgcctggc gaatccacca ggtggcataa gtggaaaact tgtacccacg
     1561 acggtattcg aacttgtcca ccgccttcat gagaccaatg ttgccttcct ggatcagatc
     1621 gaggaactgc agaccacggt tggtgtactt cttggcgatg gagatcacca gccgcagatt
     1681 ggcctcgatc atttcacgct tggcgcgcag cgtgcgcgct tcgccggcag acatctgccg
     1741 attgatttcc ttgatggccg cgatgggcag cccaatgcgc tcctgcagcg ccagcaactc
     1801 attctgccgt tcaaggatgg catactggtg gcgcaggatg ctctcgctaa atgcatggcc
     1861 ggcaccggcc tgttcgttga tccattccag gttggtttcg ttgttgggaa acaccttgat
     1921 gaaatgttcg cgcggcatgc cggcccgcct gatggcgatt tccatgatct cgcgctcatg
     1981 cgcgcggatg gtttcgacca ggtcgcggat gatgttgcat aacgattcca cctgcttggc
     2041 ggaaaaacgc aattccatga gcagaccgga aatttccagc tgaatgtcgg catattgctt
     2101 gctgccgtgc ccatgccgct tgtgtgcagc ctgcatgcgc ttgaccagcg cgcgcaagcg
     2161 ctcgaaacgc tccatggcat cagccttgag ctgcgcgaga ttggcggccg ccagcgcgct
     2221 gccgtcaata tcgtcgtcgt cgtcctcgtc gagatcttcg tcagctgcct cgtcatcgtc
     2281 ttctggagaa gcggcttcct gggcttccgc cagatcagcc tcgctggcca cgataccgtc
     2341 gacgaattca tcgatgcgga tctcatcgcg ttcgaccttg tcgaccagcg acagaatctg
     2401 ttccagcgtc gtgggacagg cggaaatcgc ctgcaccata tgcttgaggc cgtcttcgat
     2461 ccgtttggcg atctcgatct cgccctcgcg cgtaagcagt tcgacggtac ccatctcacg
     2521 catatacatg cgcaccggat cagtggtacg gccgaattcc gaatccacag tagacagcgc
     2581 cgcttcggct tccgccacca catcctcatc cgccacggcc ggggctgcgt ccgacatcag
     2641 cagcacctcg gcatccggtg cctcgtcata cacctggatg cccatgtcgt tgatcatgcc
     2701 gaccacacct tcaatctgct cggcgtcgag catgtcgtcg ggtagatggt cgttgatttc
     2761 ggcgtaggtc agatagccgc gatccttgcc gaggatgatc agcgtcttga ggcgggcgcg
     2821 gcgcagctcg gcatcgtttt cggacaagtc accgtcctgg cccgccatgc cctgtacggc
     2881 gcgttccggg ttcaaatcca tatcatccgg tttcatggtg ttccgttctt ccttgtcccg
     2941 attgttcgcc ataaccattt ccaaaaattg gatcgtccgt tattcctcga cgcaccgcgg
     3001 cgcctcttgc cgatcacaga cccgtcagcg catttcgcgc agctcctgtt tctccgcttc
     3061 actcagttcg cgcaacggct tgctggccag ccactgcaaa cgcgactgcc gccgactggc
     3121 gcgcagctgg tcgatggcca cctcaaaatc tgcggcggca tcaaatgcct cgtcccagtt
     3181 catgatctcg gctgcacatt gcgccagcac gcctcccgcc tcggtttcac gcccgcgttc
     3241 caccagtgcc ggggtatcca gttggggctg atctgccaga atttcaagca ccgtgcgcaa
     3301 tgcacggcta tcttcatcgt tgccgacaat ccactgcgcc tgcacgccag cagcaaacgc
     3361 cgggcgaaac agcaccaggc gcagcaactt ccggctcact ggcgcaggtg ccggccttcg
     3421 cgcacgcgcc ggtgcaggcg caaccggtcg ccgggtttcg atctcccaca acgccgcgag
     3481 ttcctcggca tcgatctgcg ccagttccgc tgcccgcctc cgcagcatca gcgccagcgc
     3541 ggacgcgccc acctgggcaa caagcggccg cagcgcatcg aggaaggcac tgcgcccttc
     3601 gtccacgccc aggtcgtgca actgcgacat gcggcgaaac aggtaagctg acagtggcac
     3661 ggcatcctgc tgcaaccggc tttcgaacgc ctcccggcca aactcgcgca catagctgtc
     3721 cggatcgtgc tcctgcggca ggaacagaaa cgccagacgg ctgtgatcgg tcagcaccgg
     3781 caggctctgt tccagcgccc gccaggcagc gcgctgaccg gcggcatcac catcgaaaca
     3841 gaacaccact tcgtcgctgt gacgcaacag tttctgcaca tgcactgccg tggtagccgt
     3901 gccaagcgtc gccacagcat attcgatccc gtgctgcacc agcgccacca catccatgta
     3961 accttccacc accagcaccc ggccggcatc gcggatcgcg cgccgggcct ggaacagccc
     4021 gtacagttcc tgccccttct ggaacagcgc cgtttccggc gagttcaggt atttcggctc
     4081 gccgtgatcc agcacccgcc caccgaaacc caccacatca cctttctggt tcaggatggg
     4141 gaacatgatc cggtcacgga aacggtcata acgttttccc gcctcgctgc tgatcaccag
     4201 accggtctgc gcaagcaccg catcttcgta gcgttcaaac gccacagcca ggttctgcca
     4261 gccatctggc gcataaccca gcccgaactt cgccgccacc tcgcccgtca acccacggcg
     4321 cttgagataa tcaatggcgc gcgttgacgc cttgagctgc tgccggtaat accgactggc
     4381 gactcgcaac gcctcgacca tgccgggtgc gctgctgctc cgcgactcat gccgcaccgc
     4441 attgtcctgc ggcaccggca tgccaagttg ctgcgccagc gcctggatgg cctccacata
     4501 tcccaacccg gagtactcca tcagaaaacc gatcgccgtt ccatgcgcac cacaaccgaa
     4561 gcagtgatag aactgcttgg tgggcgagac ggtaaaggaa ggagattttt cctggtggaa
     4621 cggacaacag gcctggtaat tggctccggc ttttttcagg ggcacatggc gaccgatcac
     4681 atcgatgata tcgacacggc tcaacaaggt ttgaatgaaa tcctgcggaa tcattgcaac
     4741 gccttcacca ctcgcgccag cggcacatcc atcccatccc gcgtttcacg cgcggagtca
     4801 acctgccagt ttcgccttga tccgcgccga aacctgcgcc atatcggcgc gccctgccag
     4861 tttcgggcgc acttcggcca tcaccttgcc catgtccttg acacctgccg cgccactcgc
     4921 ctgcatggcc tgcgcgatca ccgcgtcgat ttcttcctcg cctgctgccg ccggcatgta
     4981 agactgcaac acttcggcct cgaaccgctc ggcagccacc agatcctcac gcccggccgc
     5041 ctcgaactga ctgatcgaat cgcggcgctg cttgagcatc ttgtcgataa tggccaccac
     5101 ctgcgcatca tcgagcacga tacgctcgtc aacctcgcgc tgcttgagtg ccgcaagaag
     5161 caggcgcaac gcacccagcc gtgcgctctc tttcgcgcgc atggcggatt tcatgtcttc
     5221 ggtaatacga tccctcaggt tcattcgttc catccaggcg cacgcggggc gcaccccgcc
     5281 ttgtgaagac ggtcaaacca gcctgtaaat tccggtctgc ccaaatttac gcgggggcca
     5341 tccccgccac tctgccgcac cagccaccat gggcggccgg cagcgacaat cagtacagct
     5401 tgggcggcaa catctggcta cggatacgct tgtagtgccg tttcaccgcg gctgccagtt
     5461 tgcgcttgcg ctcggcagtg ggcttttcgt agaattcgcg ggcgcgcagt tcagtcagca
     5521 gcccggtctt ttcaaccgtg cgcttgaagc ggcgcattgc gacttcaaaa ggttcgtttt
     5581 ccttgacgcg gacactaggc atgtacggtg aactccagaa ttttgggggg aagaaaggcg
     5641 gcattataat ccattgccac cagatttcca acccggccgc tgacaaaatt catgccgcac
     5701 tcggtacgcc atcctggcgg cgcagcgcaa catccacgtt ttcagggtag aatacctgca
     5761 tgagttatca agtgctggcg cgcaagtggc gcccccggac ctttgccgaa ctggccgggc
     5821 aatcccatgt ggtacgcgcg ctcggcaacg cgctggaaca gaatcgcctg caccacgcct
     5881 ggctgttcac cggcacgcgc ggcgtcggca agaccacgat tgcccgcatt ctcgccaagg
     5941 cgctcaactg cgaaaccggc atcacggcac acccgtgcgg tgaatgcacg gcctgtcgcc
     6001 agattgacgc gggccggttc gtcgatctga tcgaactcga tgctgcttcc aacaccggca
     6061 tcgacaacat gcgcgaagtg ctcgacaacg cgcagtacgc acccacggcc ggacgcttca
     6121 aggtctacat catcgacgaa gtgcacatgc tgtccaaagc cgccttcaac tcgatgctca
     6181 agacgctgga agaaccgcca ggacacgtca agttcattct tgccaccacc gacccgcaaa
     6241 aaattcccgt cacggtgctg tcgcgctgcc tgcagttcaa cctgaagcag atgccgccgg
     6301 aactcgtcgc gaaacatctc gaacacattc tgtccgaaga acgcatcccg gccgaagccg
     6361 ccgcgctgca gcgcatcgct cacgcagcgc atggcagcat gcgcgatgcg ctgtcgctgc
     6421 ttgaccaggc catcgcctac ggcggcgagc aggtgctcga tgccgacgta cagcatatgc
     6481 tgggtggact ggaccgcagc gacctgttac aactgctcga cgcactggcg gaccgtgatg
     6541 gcaccgccct gctcgagcgc tcggcccgac tgctccagca aggcgtcgat gccgcatccg
     6601 cattgcagga aatcgctcgc ctgctgcatc agatcgcgct gttccagact gtaccggaag
     6661 cgctggacga ggcgctggac gaacgcgccg ccattgaggc cctggcggcc cgactggaca
     6721 gcgaaaccgt gcaactgtat taccagatct gcctgctcgg ccggcgcgac ctgccactcg
     6781 ctcccgatga aactgccgga ttcaacatgg tgctgctgc
//
LOCUS       DAIANL010000020         3688 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1039303, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000020 DAIANL010000000
VERSION     DAIANL010000020.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039303"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          664 a         1030 c         1307 g          687 t
ORIGIN      
        1 ttttcaggtc aggaaattgg caaggcgctt tccagtccga agcgtctgga actgatcgaa
       61 ctgttgagtc agggggaaaa ggctgttgac aggatcgcca gggaggccga gatcagcgtc
      121 aagctggcca gtgcgcacct gcgcgagctc aagctggcgc atctggtgga gacgcggcgc
      181 gagggaaagc acgtttttta ccggctggcg gatgagcggg tggcggattt ctggggcgtg
      241 ctgcacgggc tggctgaaaa ccggctggtc gaattgcgcg cggtattgag cgagatcgcg
      301 ctggcgcccg agaaattggt acccagcaag cgagaggcgc tgatgaaagc ggcacgcaat
      361 ggggaagtga tcgtgctcga tgtgcgtccg ctggaagaat acaatgccgg gcatctgcca
      421 tttgcccgat cgatcccgtt aggggaatta cgccagcgcg tggacgaact tgcgccggac
      481 cggccggtgg tggcgtattg ccgtggcccg ttttgcctga tggcgcgcga tgcagtaatg
      541 tggctgcgcg ggcagggcta ccatgcggcg caactgaccg atggcgtggt cgaatggcgg
      601 cttggccagg cgcaggatca gcgtgctgtg gattgaccgg acagattgcg tcacctatga
      661 tgaatccatc atgaatgtac tgctgcgttt cctgctggtc tgtgtgctgt gcatttccgt
      721 accggaatat gcgctggcga cggtcgaggc cgcgcgtggc agcgatgaaa tctgcctgat
      781 gcagagctgt gccatgacaa tgatgcaaga tggggcgaca cggcacacga acgcgggtca
      841 cgcccgtcag catcatgcca cgacctgcgc ttactgcaag gtctgcagcg ccagtgctgc
      901 ctggcagccg gctgtgccgg tgccggctgg cgtgctgacc gcgtgtatcc gcatcacctc
      961 gcccgggttg tcccagcccc gcctgtcgca ggatccggaa ggattctggc gcccaccacg
     1021 ccgttcctga aactgttttg ccgtgtatcc gtcgcgttac gcgtgacgga tgattgctca
     1081 cgttttcagg agatgatcat gtatcagaat gcccaggcgt tgtccgcgct gatgctgtca
     1141 gtgctgtcag tgctggctgt gctggcgcat gcggcaccga tcgatggaat gtcgccgcag
     1201 acagcgtcgt cccgtactct ggcaatggtc acgccgtcaa ctacgcctct gcggctggat
     1261 gaggcggtca tgctggccgt gcgtgaatca gcgtcgcttg cgggtcagaa tgcctatgtc
     1321 gaggctgcgc gcaagcggga ggtttccgca ggcagcctgc ctgatccaaa actgtttctc
     1381 ggactggaca atctgcccgt caatggcgca gaagcatgga atacccaggt cgatccgatg
     1441 accatggaaa ccgttggtgt gatgcaggac atgccgaacc gcgcccggcg tgacgcagca
     1501 acgggggtgg cggtggccca gaccgcagtg gaaacagcca gatggcgtgc gctacgcgtg
     1561 caggtacgga gagaggcagc gcaggcatgg atcgggtgtt actggctggg gcgcaagttg
     1621 gttctgttgg atgcgctcga ccaggaaaat cacgccctgc aacagatgct gcaggctcag
     1681 gtggccgctg gcagcgcccg tgcgacacaa tgggtgctgg cgcaacagga ggcggcggaa
     1741 ttggctgacc gacgtgatgt cctgcaacag gagtcgagtc aggcacggat cgcattgcgc
     1801 aagctgatcg gcgcgcgggc agatgcgccg ctggccgacg atccgccgtc gttggtgccg
     1861 gatgcgcaag cgctgcgcgc ctcgttgcgc gaccaggcgg acctggccgt gtatgcgccg
     1921 atggaacaga aagcccgggc agaggaaggc ttggccgaag cggcaaaaca gtccgattgg
     1981 ggcgtatcgc ttgcctggca gaaacgcaat ccggcttacg gcgacatgat gtcgttgcag
     2041 ttcacttata ccttgcccgt attttctgcc acacgcaaag atccagatat tgccgccagc
     2101 aagcaggaac gtctggctgt tgtcgaccag cgcgagcagg cgctgcgtga tcggcgcgcc
     2161 gcgctggaaa gcgagcttga tcaataccag accctgcagc gacagatcga ccgcatccgt
     2221 caggtatggt tgccgctggt gcagcagaaa caacagttgc aactggcggc ctaccgcgcc
     2281 aatcgcggtt cggccgatgc ggtgatcgaa gcccggcggg atgcgatcga acagcagttt
     2341 cgcctgctcg acctgttgtc gcggcgtgat gcgctggggg cacagttgca ttatgtctat
     2401 gcgcaaggag cgtcgtcatg aaccggcgtc aggcaacgat ctggatgatt tcgcttgttc
     2461 tggcctgcgg tttgggagcc tgggcaggtt accggtacgc cggacggggg gtgcaagcgc
     2521 ccgtcgcgac ccgatcgact gccgtcagtg gcgcgcgcca gatcctgtac tggtatgacc
     2581 cgatggttcc tgaccagcat tttgccaaac cgggcaagtc gccgttcatg gacatgccgt
     2641 tacagccgaa atatgccgac atggcgggcg cgccacacgc tgtgcacatc gatccggcga
     2701 tgagcgaaag tctggcggta cgcgagacga cagtccgtcg cgggatgttg cggcaacgtg
     2761 tggatgcgcc tgccataatc gcctgggatg accggatgca ggccgtggtg cagctgcgca
     2821 ctgccgcgtt tgtcgagcgc gtctacggcc ggtcggttgg tgaccgggtc ggacgcggcg
     2881 cgccgttggc acaggtgctg gtgcccgact gggcgggcgc gcagcaagaa tatctggcag
     2941 cacgagccac gggcgatgcc gagcttgcag cggcggcgcg cgaacgcttg atgctgctgg
     3001 gcatgaatgc ggcacagatc gcgcaggtgg accgcagtgg acaggttcag gcgcgcatca
     3061 cgctcactgc gccgctggca ggcgtgctgc aggaagtcaa tgtgcgtacg ggcatggcgt
     3121 tgtcggcagg ggccaccgtg gcgcgcatca acgctgtgga tccggtatgg gtcgaggcag
     3181 aagtgcccga ggcgcaggcc agtcaggtgc gtgtcggcca ggtgatgcag gtgcgtcttg
     3241 cgtcggctcc ggggacggtg cgtcagggca aggtgatcga ggtgctgcct gaggttgctg
     3301 cggacacccg cagcgtgcgg gtgcgggtac aactgcccaa tgcggatgaa gcgctcaggc
     3361 cgggaatgta cgccaccatt cgtctggaga acccgatggg cccggccggt gtgctggtgc
     3421 cggccgaggc ggtgatccac accggaacgc gcgatgtcgt tatcctgtcg ctgcacggtg
     3481 gacagcgttt cgtgccggtt gaagtcaaaa ccggggggca ggggggtgac gaagtgctgg
     3541 tgttgtcagg gctgcagccg gggcagcgca ttgtacgttc gggggaattc ctcattgatt
     3601 ccgaagcgag cctgaccggc gtgctggccc ggatgaatag cgccggcgcc ccgcatgtca
     3661 tgccggccat ggttacgccg ccggcgac
//
LOCUS       DAIANL010000021        12605 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1855706, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000021 DAIANL010000000
VERSION     DAIANL010000021.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12605)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855706"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2579 a         4117 c         3532 g         2377 t
ORIGIN      
        1 aaagttaatt attttaatta ttacgttaag ttattattta atatttattt tgaaccaccc
       61 gatctgtcca tgatttcagt cctgcaatcc cgacagaatt ttcatggcag cggcggcaaa
      121 aacattatca aagagcagca aaataaaaaa aattaaaata tatctaacgt aatgttttac
      181 attagtttaa taacgtgatg ccccattcca cacggtcgcg acgctggcct ggcgggatgg
      241 attgcaagac gcgatccgtg gagcatcagt caccgcgtcc ctaatggttg aagcggctta
      301 cagtctggca gcgcattttt gaaggcacgc gctttgcaca agtgcattct tccgccaatg
      361 acatgcgctc atcagcacat tgccctggcg gcaaacgtgt catcactgga cggaagaggc
      421 cgaaccaggc gtctgtcagg cagacgaata ccgcgcacat ggcagcatca gccagcaagc
      481 acgccggatt gtgcaacgac acgaccgcgc aacatgaaat caggaaattg catcagctga
      541 caggcggcag atatgcccct tgggatgaga ctgccggcga gtgacgacga aaatcgggtc
      601 agggtgccgg gcgaaccgta aaccgcctga acactccagg caatgccgtc tttgcagcac
      661 aaggcgaact gacgtgcgcg cacgtcaggt aacagggtgc agaaatggat atgacgatgc
      721 ctcgggcgag gcatctccga tcgagactgg tcaggcttcc ggattcaaat cagtctgctg
      781 aagcgcgtta ctgtcgcgca tgccgcaaat tctccggcag ccccctgctg aaattgcaac
      841 gccaggggtt gatgtccagc ccgccacgcc gggtatagcg cgcatacacc gaaagccgtt
      901 gcgggcggca gcggcgcaag atgtcgcaga agatctgttc cacgcactgt tcatggaacg
      961 cgttgtgatt gcgataaccc accagataac gcaacagtcc ggcctggtca atcggcgcac
     1021 cggaatagta cacctgcacg ctggcccagt ccggctgccc ggtcacagga caattggaac
     1081 ggaacaaccg ggtcaccagc gtctgttcca ccggctcggc gccagattcg ctgatcagca
     1141 cctgcgcatc cggcacacgc agatcgcatg ccacatccag ccggtcgagc agcaatccgc
     1201 ccagttcagt caacggctgg ccttccagtt cgtcaggcga ttgcagttgc acggccacgc
     1261 tggcgcccgc cgcctgcgac aggtcggcag caatgcgctg gcgcacttct tcagcgtcgg
     1321 tgcaacgggt ttcactgaat gcattcagat acagcttgag agatttcgat tcgaccagat
     1381 gcgacgattc cgccggaaac accagcctgg ccagcgccac ctgcggcttg ccgcgcgcat
     1441 tcagccacga cagctcatat gcattccaga tgtccgcgcc aataaacggc aggctggctc
     1501 ccaacccgag ctgagcccgc aacggtgcgc gttcgacggc atacagcaac ccgggatcat
     1561 actgcacggc ataggtaacg gctttgccaa gcggcgcgtc cgacaggccg tcatcatgca
     1621 tcaggcagcg ccagcacata tggcaggtca cgccgtgtca gcgccacgcc ctgcaatgat
     1681 cctgcaaaca gcggctgatt ttcgacactg gaaatctgcg ccaccaccaa cgcatcccag
     1741 ccccccgcat gtgccggctg ggcatctacc accatgccgc tcgcctggcc ttccatatcg
     1801 gggctgaaca ccggctcgcc cggagacaac tcgccggcgg catgcaccag atacatccgc
     1861 cgcttgagtt tgccgagata ctgcatgcgt gccacgatct cctggcccgt ataacagcct
     1921 ttcttgaaat ccaccgcctg aatcagatcg aaattcgcca tctgcggcac gaactcttca
     1981 cgcgtctcgg gcaagatgcg cggcacacct gcacggatat ccagcaagcg ccattgcgca
     2041 gcgtccagcg gcgacaagcc gtccagccat gccggccgtg ccgcacctgc cggcaacacc
     2101 accacaaagc gttgcgcatc gagccggatc acactcacat cgcccagttg caacacggcc
     2161 atgcctgcgt ccggtttacc gccaagtgcc tgatcgatgc acgctgcggc atccggcccg
     2221 acaatgccat aacgctgcca ttgcccgcca gcctctgtca gccgcacttt ggaacgcagc
     2281 acgtacatcg acagtcgctt ctgcacgaac tcgcgctgac tgtcaggcaa ttgcaagtac
     2341 aggcgatctc cgcgccgcca gatcaggaaa ctggcaagca gccgcccctt gggcgtacac
     2401 aagccactgt actgcgctgc cgtcggcgtc accttgcgca catcgctggt caactggcct
     2461 tgcaaaaagg tcacggcatc gtcgccgctc acctcgatca gtccttccat ggacaggtcg
     2521 gcgcacgcca cacctgtccc gaatccatca cccgaatgtt ccattgttgt catcctgcag
     2581 gtctgtccct tcgcgggaac gagccggccc attctagcaa aagcatcgac acaaaacaaa
     2641 aagccgactc aacgagtcgg ctttttgtgc ctgaggcttg ccggattact tggcggcagg
     2701 agcagcagga gcagcggcag gagcagcggc aggagcagca gcgccagcgt cggcaggggc
     2761 agcggcagga gcagcagcgc cagcgtcggc aggagcagca gcaggagcgg cggcaggagc
     2821 agcggcacca gcgtcagcgg gagcagcagg agcagcggca ggtgcagcag cgccgttgtc
     2881 ggcaggagca gcagccggag cagaggtgtc aggagcagga gcttcttcct tcttaccgca
     2941 agcggtcagg gtcagagcca tcagggcagc gatcagcagg gaacgggtca ttatggatct
     3001 ccagtttttc aaatcaaaaa caattcagat aacttgcata cgccaactaa tggcacaagt
     3061 cggacaaacg aaacatcaat tcgttcgtgg caaaattata cgcaaaaatt tttatttgtg
     3121 cgtgaatgtt gtttattttt ttcacgtaaa tacaggctat gcctcatgtt agaatagcag
     3181 actcaggata aaaccattcc gtcaacccga atccaggatg attccatgtt cgaccgcaaa
     3241 ctcagcatcg ctgtcaccga ccctgccgtc tggcagacca ttcagaacga aacccgccgt
     3301 caggaagacc acatcgagct gatcgcttcc gaaaactaca ccagcccggc ggtcatggaa
     3361 gcccagggca ccgtgcttac caacaagtac gcggaaggct atcccggcaa gcggtactat
     3421 ggtggctgcg aatatgtcga cgtcaccgaa cagatcgcca tcgatcgcgc gcgcgaactg
     3481 ttcggcgcgg aatatgccaa cgtacagccg cattccggct cgcaagccaa tgccgccgtc
     3541 tacctgtccg tactcaagcc cggcgatacc attctcggca tgtcgcttgc ccacggcgga
     3601 cacctgacgc atggcgcgtc ggtcaacttt tccggcaagc tgtttaacgc catcagttat
     3661 ggcctgcacc ccgaaaccga agaaatcgac tacgctgaag tcgagcgtct ggcgctcgaa
     3721 caccgcccca agatgatcgt cgccggcgct tcggcctatt cgctggtcat cgactggcaa
     3781 cgcttccgtc gcatcgccga cagtgtgggt gcgctgctgt tcgtcgacat ggcgcattat
     3841 gccggcctga ttgctgccgg ccactaccca tctccggtcg gcatcgccga tttcgtcacc
     3901 accaccacgc acaaaacctt gcgcggtccg cgtggcggcc tgatcctggc caaagccgaa
     3961 tatgaaaagg cgctcaactc gaccattttc cccggcaccc agggcggccc gctcatgcac
     4021 gtcatcgcgg ccaaggccgt ggcgctcggc gaggccatgc gcgacgactt caagacctac
     4081 cagggccagg tgatcgccaa cgcgcgcatc atgaccgaag tgctcaccag ccgcggattg
     4141 cgcatcgtat ccggccgcac cgactcgcat ctgttcctgg tcgacctgcg cgccaaaaac
     4201 attaccggca aggatgccga ggcggcactg gggcgtgcgc acatcaccgt caacaagaac
     4261 gccatcccca acgatctgca gaagccattc gtcacctccg gcatccgcat cggcacaccg
     4321 gcaatgacca cgcgcggttt cggcgcccag gaggcccacc agctcgcgca cctcatcgca
     4381 gacgtgctcg acgcgcccac cgacgaggcc acgctggcga aatgctcggc cgctgcccag
     4441 gcgctgtgca cccgcttccc cgtttacggc tgacgaggcg acaggcggca tgaaatgccc
     4501 gttttgcgga gccgaagaca ccagcgtggt ggattcgcgc gtcaccgagg ctggcgatgc
     4561 cattcgccgg cgcaggcgat gtctgcaatg cgacaagcgt ttcaccacgt tcgaaactgc
     4621 cgagctgcgc ctgccacaag tcatcaagtc gaacggcaac cgcgaggaat tctcgcccga
     4681 caagctgcgc gagggcttcc agcgcgccct gcacaagcga ccggttccga ccgaatacgt
     4741 cgaccaggcc atccaggatg tcatcaagca ggtgctcgcg cagggcgaac gcgaaattcc
     4801 ttcgcggcag atcggcgaaa tggtcatgca ggccctcaag ccactcgaca aggtcgccta
     4861 catccgcttt gcctcggtgt acaaaagctt ccaggacgct gacgacttcc gccacgtcct
     4921 gctggacctg gacacatgac caccggtgcg gcatggagca gcgccgacca cgcccacatg
     4981 gcgcaggcgc tgcagctggc ggagcttggt ctgtacacaa ccacccccaa cccgcgcgtg
     5041 ggcttcgtca tcgtgcgcaa cggcaccgtg gtcggtaccg gctggcatcg ccgcgccggc
     5101 gaagcccatg ccgaaatcca tgccctgcgc gaagcgggcg aacgcgcacg tggagccacg
     5161 gtgtacgtca cgctcgaacc gtgcagccac cacggccgca caccgccatg cgccgacgca
     5221 ctgatcgccg ccagcgtagg ccgtgtggtg gttgccatgg ccgatcccaa cccgctggtt
     5281 gcaggacaag ggctggcccg actggccgca gcgggcatcg acaccgcgtg cggtctgctc
     5341 gaatcagccg cacgccgcct caaccccggg ttcatccacc gcatgacaca ccagcgccct
     5401 tggctgcgca tcaagaccgc cacctcgctc gatggccgta ccgccctggc caacggcgtc
     5461 agccagtgga tcaccggcaa tgaagcgcgg ctggatgcac accgcctgcg cgcacgctct
     5521 tgcgccgtcc tcaccggcat tggcacggtg cttgccgaca atccgcaact caccgtgcgc
     5581 ggcatcgacc ccccacgcca gccagcgaaa atcatcgtcg actcccacct gcgcacgcca
     5641 cccgatgcgc aactgctaca atcgggcaac gtactgatcg ctcataatgg ccagcacgct
     5701 gaacgcgaag caccgctgcg cgacgccggc gcgcaactgc tggcagtgcc cggtgcgcaa
     5761 gggcgggtcg atctgccgcg cctgtttggc atgctggcac aacacggcta caatgaaatc
     5821 accgccgaag ccggcagcac cctcaatggc gcgctggtcg ccgccaggct tgcagacgag
     5881 tggatcggct atttcgctcc gatgctgctt ggcaaccagg cgcaggggct gttcgcactg
     5941 ccctccctgg tcgggctcgc agatgccacg cgcctgcaca tcggcgagat cgtgcgcatc
     6001 ggccaggact ggcgactgac ggcacatttc gtacctacac cgcgctgaga gaagtacgtc
     6061 tatgctctgg atgcaccttg cgggatttgt gctgctgatc cttgccgctt cggaaatctt
     6121 cgtcaatgcc ctcgaacatc tcggtgaacg gctcggtatt tccgaaggcg taaccggttc
     6181 gctgttcgcc gcagtcggca cggcgctgcc agaaaccctc gtgccactga ttgcgctgct
     6241 ggccggaacg gccgacccgc acgccaacga ggccattggc accggcgcca tcctcggcgc
     6301 acccctcatg ctgtccacgt tatcggtctc gctactcgcc ctggtcaccc tgcgccgtcg
     6361 cggttttcag ggtgaattcg cgccggaacg cagcggcatc gtgcgcgatt tcaacatgtt
     6421 cctcgccagc ttcagccttg cctgcgcagt catgttcatc ccgcacggca atgcggccct
     6481 gcgactgggc tttgcctttg tgctgctgct gggctatttc ctgtatgtac tggttaccct
     6541 gcgtgcctct gcgcatctgg tgcaaagcgg acatggcacg gaggcgcatg cgccgctgat
     6601 gctgaccagg atcggcctgc ccgaccacat ggctgtgctg ctgtttcagc ttgctgccgg
     6661 cattttcatg ctggtgcaag gggcggatgg ctttatcgtc gccatccacg atgcggcccc
     6721 gctgctgggt atttcaccac tgttgcttgc attgctgatc gtgcccgtcg ccacagaact
     6781 gccggaaaaa gtaaacagta tccgctgggt gcgccacaga cgtgacacgc tggcattcgg
     6841 caacatcacc ggcgccatgg tgtttcaggg ctcgatcctg cccgccatcg gcattgccct
     6901 gaccgactgg caaccgcacc gcgaaatcat gcccggaatc gtctgcacac ttgccggcgc
     6961 gttatggctg cgactgtgtc tgatgcgcgg ccacctgcgc gtgtggcaac tgggcgtcaa
     7021 tgctgcgcta tacgcaggct atcttgccat cagcctgcac tgagccacca tcctcgtctg
     7081 cgaacagttt cttgacaact tctttacatc ccgcctgaca agttttaccc cgttttgacc
     7141 tcccgatgcc tagcatggca ctcatggaaa accgctcggg agaaaaccgc catggaactg
     7201 ctctgtcttg cgctgatggt cgccctgttc ggcctgtcgg tgctactggt ttacgcttgc
     7261 aaacagctcg ggagaccaca atgacctgga tctacctgct ggccggcgcg ctggttgccg
     7321 gattgctgat ctacctcgtt gtcgccctgc tcaagccgga gttgttctga tgactaccaa
     7381 tggactgtta caaatcggcc tgttgctggg cgcattgttc gcgctcgtca agcccctggg
     7441 catatacatg acacgcatct accagggcga cttgccggca ttcattcgct ggctgtctcc
     7501 actggaacac atctgttatc gggctggtgg tatcaaagcc gatgacgaaa tgggctggcc
     7561 acgctatgcc atgagcctgc tgtggttcag cctgtccggc gtgctggcgg tatatgccat
     7621 acagcggctg caaggcgcgt tgccattaaa tccgcaacac tttggcgccg tttctccgga
     7681 ttcggcgctc aataccgcga tcagtttcgt caccaatacc aactggcaag gctacgctgg
     7741 cgaatccacc atgagctacc tgacccagat ggtcgccctg accgtacaga actttttttc
     7801 tgctgcatca ggcatggctg tggccatcgc actgatccgc ggcttcgccc gccatgccac
     7861 ggattcgatc ggcaatttct gggtggacat gacgcgcagt acgatctata tcctgctgcc
     7921 gctgtcgatg gtcctgacgc ttgcgctggc aagtcagggc gtggtgcaga acttttcccc
     7981 ctaccagacc atacagatgg tcgaaaccgt ccggtacgac aatccgcgga tcgatgccaa
     8041 cggcaacccg atcagggatg ctgccggcaa cccggtcact gacagactgt ccacccggga
     8101 acagaccatc ccgttcggcc cggtggcttc gcaggaggcc atcaaggaac tcggcaccaa
     8161 tggcggcgga acgtttaatg ccaattccgc gcatccgttc gaaaacccga ccccgctgag
     8221 caactttctg caaatgctgg caatcctgtt gatacccggc gccctgtgct acaccttcgg
     8281 acgcatggtc ggcgacaccc ggcaaggatg ggccattctt gccgccatga cgctggtatt
     8341 cgcctgcatg ctgtcggtgg ccgaattcgc cgagactgca ggcaaccccg tgctggcggc
     8401 gctgggtatc gaccagtcgg tgacgccgga acagtctggc ggcaacatgg agggcaagga
     8461 aacccgtttt ggcacggtca attctgcgct gttcgccaca atcaccacgt ccacctcctg
     8521 tggcgctgtc aacagcatgc atgattcctt caccccgctg ggcggactgg tgccgttggt
     8581 caacatccag cttggcgaag ttattttcgg cggcgtcggt tcgggactgt acgcgatgct
     8641 ggtgtatgcc gtgatcgcag tattcatcgc cgggttgatg atcggccgta cgcccgagta
     8701 cctgggaaag aagatcgaat cgttcgacat caagatggca tcgctcacca ttctcatccc
     8761 gcccctgctc atcctgtgcg gcacggcaat cagcgtgatg gtcgatgcgg gccgggctgg
     8821 cgttgccaat cccggcgcac acggattttc cgaaatcctg tacgcgttct cgtccgcagt
     8881 cggcaacaac ggcagcgcct ttgccggcct gtcggccaat acgccgttct acaacattgc
     8941 actggccgtt gccatgtttt tcggccgctt ctggctgatc atcccggtgc tggcgctggc
     9001 gggctcgctg gccggcaaga aacgcattgc ccccggcgca ggcaccatgc ccacccacac
     9061 gccactgttc gtcatgctgc tgatcggcac ggtcacgctt gtcggcgccc tgactttcgt
     9121 gccggcgctc gggttgggcc ccatcgcaga acagttcacc atgaccagcg ctcatcagga
     9181 gattccccat gactagcaat tctctcaccc tgagcgaccc gaagatactg aagcaggcac
     9241 tgtgggacgc gctgcgcaaa ctgtccccac gccaacaggt aagaaacccg gtgatgttcg
     9301 ttgtctggat tggcagcctc ctcaccagca tcctgttcgt tcagtcgctg tctggtcacg
     9361 gtgaagcccc ttcaaatttc attctgagca tctcgctgtg gctgtggttc accgttctgt
     9421 tcgccaattt tgccgaagcc atcgccgaag ggcgcagcaa ggcgcaagcg gcttcgctgc
     9481 gccaggcccg gcgcgacgtc atggcaaaaa agctcgacac gccacgccat gatgccaact
     9541 ggtcaaagat cggcagcagc atgctacgcc gcggcgatgt ggtcctggtg gaagccggcg
     9601 acatgatccc tgcggacggc gaagtgatcg aaggcgtcgc ttccgtcaac gaaagcgcca
     9661 ttaccggcga atcggccccg gtcatccgcg agtcaggcgg cgacttcaac gcggttaccg
     9721 gcggcaccac ggtactgtct gactggctgg tggtgcaggt aaccgccaac ccgggcgaaa
     9781 gttttcttga ccgcatgatc agcatggtgg aaggcgcgaa acgacagaag acacccaatg
     9841 aaatcgcact gaccattctg ctggtggcca tgaccctcat cttcctgctg gccaccgcca
     9901 cgctgctgcc cttttcgatt tacagcgtgg cggtgaccaa aaccgggcat ccggtcacca
     9961 tcaccgttct ggttgcactg ctggtctgcc tgattcccac caccatcggc gggctgctgt
    10021 cggccatcgg tgtcgccggc atgggccgca tgttgcaaaa aaacgtgatt gccacttccg
    10081 gtcgggccat agaggccgct ggcgacgtgg atgtgctatt gctcgacaag accggcacca
    10141 tcacacttgg caaccggcag gcttcgacct ttttcccggc ggaaggcgtc agtgcggccg
    10201 aactggccga tgcagcccaa ctcgcatcgc tcgcggacga aacccccgaa ggccgatcaa
    10261 tcgtggtgct cgccaaacag gaattcaatc tgcgtgaacg cgatgtccat gcgctcaatg
    10321 ccacgttcat ccccttttct gcacataccc gcatgagcgg tgtcaacctg ggcgaacgcc
    10381 agatccgcaa aggagccgcc gatgccatga caatccatgt cgaggctttg ggcggcacat
    10441 tccctgaccc gatgtcccat ctggtggacg acgtagcccg gcgtggcagt acgccgctgg
    10501 tagtggctga tggtgcgcgt gtcatgggtg tggtggaact caaggacatc gtcaagggcg
    10561 gcatcaagga acgctttgca gaactgcgcc gcatgggcat caaaacagtc atgatcactg
    10621 gcgacaaccg actgacggcc gctgcaatcg cggccgaagc cggcgtggac gattttcttg
    10681 ccgaagccac gccagaaacc aaactgtcgc tgatccgcaa gtaccaggcc gaaggtcggc
    10741 tggtggccat gacaggcgat ggcaccaacg atgcgcctgc actggcccaa tccgacgtcg
    10801 ccgtggcgat gaacagcggc acgcaggcag ccaaggaagc cggcaacatg gtcgatctgg
    10861 attccaatcc caccaagctc atcgaaatcg tcgaaaccgg caagcagatg ctgatgactc
    10921 gcgggtcatt gaccaccttt tcgatcgcca acgacgtcgc caagtatttc gccatcattc
    10981 ccgcggcctt cgtcaccacc tacccggcac tcggatcact caacgtgatg catctggcct
    11041 cgccagccag cgccatcctg tcggcagtaa ttttcaatgc gctggtgatc gtggcactga
    11101 ttccgctggc gatcaaaggc gtgaaatacc gcgctatcgg agcagacagg ctgctgcgcg
    11161 acaacctgct gatttacggg ctggggggca tcatcgtgcc attcatcggc atcaaatgca
    11221 tcgacatgct gctggccgct gccggctgga tttaaggaga catccaccat gttcaaagac
    11281 cttcgtccca cgctcgtcag cttcgcgctg ctcaccctga tcgtgggtat cggctacccg
    11341 ttcgtcatga cgggcctcag ccaatggctg tttcccgacc aggccaacgg aagcctcctg
    11401 ctgcgcaacg gcacaccggt cggatcatca ttgattggac agccattcag cggcgccggt
    11461 tatttctgga gccgcccatc cgccaccgct cccatgcccg acaatgccct ggcctcgggc
    11521 ggttccaatc tcgggccgac caatccggca ctggccgatg cggtaaaagc gcgcatccat
    11581 gcattgcagg cagccgatcc caacaacacc gccccgatac cgatcgacct ggtcaccgca
    11641 tcggccagcg gccttgatcc gcacatcagc cctgctgcag cgctctatca ggcacagcgc
    11701 gtagcgcgtg cacgcggtct gccgctcggg acggtacgtg ccctgatccg ccggcacatc
    11761 gaagccaggc aattcggtgt actgggcgaa gcgcgcgtca atgtgctcga actcaacctg
    11821 gcacttgatg ccttaccgca ttcctgacgc atcggtacgc gccgctgcca gcgctgctgc
    11881 tgacggacgg cgcgtcctgc tgtacgctgg cgtacagccc accctgatct gacacgcaat
    11941 accccatgcc agccaacgat agcgatcacc gacccgaccc tgacgcactg ctggagcaca
    12001 tccagagcga agtcatgcgc gcccggcgtg gcaagctgaa agtttttttc ggcgcctgtg
    12061 ccggtgtcgg caagacctgg gccatgctgt ctgctgcacg ccagctacgc tcgcaaggtc
    12121 aggacatcgt cattggtctg gccgtcacgc acggccgtgc cgaaacggaa accttgctcg
    12181 aaggcctgga acgcctgcca cttaaaacca tcgaataccg tggtcacaag cttgatgaat
    12241 tcgacctgga cgcagcgctg gcacgcaagc cggccctgat cctcgtcgac gaactggccc
    12301 acaccaacgc acccggctcg cgtcacccga aacgctggca ggatgtcgca gaactgctcg
    12361 atgccggcat caatgtcttc acgacggtca acgtacagca tctggagagt ctcaacgatg
    12421 tggtcggcaa catcaccggc atccggatcc gggaaacagt gcccgacaac gtgttcgacg
    12481 aggcagatga agtggtgctt gtcgatctgg caccagacga gttactccgg cgactgcagg
    12541 acggcaaggt atacctgcca cagcaggcaa gacacgcgct gcgcaatttt ttccgcaaag
    12601 gcaac
//
LOCUS       DAIANL010000022        43250 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_833874, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000022 DAIANL010000000
VERSION     DAIANL010000022.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 43250)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43250)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..43250
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833874"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         8309 a        11986 c        14184 g         8771 t
ORIGIN      
        1 gcttcgagcg aaaacaggtc gtaggggttg acgatggccg gaacgccttg ccgcatgatg
       61 gtgttggtga ctggatgcac ccggatctgg gcgctgtccg gtacctgttt gacgcagacg
      121 acgatatgca tgattgctcc tcaaccggca gtgggcaggg ttgatttcag cttgtccagg
      181 gagaagcttt tggcctcggc atcctgaaac accttgaaca ccttttcggt ggcagcattg
      241 gagcgtacga aatcgtcata ggcgcgttgc agcgcatcgc ggtagagcgg gaacagcgcg
      301 gcatcgtcga gtccggcagg atgccgctcc tgacgcaggt actggtagaa gcgtttcagg
      361 atgtgcaggc ggttgacacg caccacatgt tcctcgtgcg cgatgccgaa aaagtcgaga
      421 aattcctcgg cggcagaaaa ctgcctgagt tggccgatca tgtcgttcat gacagtgctc
      481 cggtaagggt ggcttgttcg agatggaacc ggcgcagatc gtcggcatcg gccgggcgtt
      541 tgttgagcat ggcgtgtgca cggatgcgct gcagcatcca gccagtctcc ggctcgccga
      601 gcaggatacc gatgcgggcg caggccatct gcaccgcatg cgaccctgaa tgcttgccga
      661 gcacagtgcg gtgttcgcgg ccgacttcgg ccggatcaaa tccctggtaa ttgagtgggt
      721 tcttgatcag tccgtcgaca tggatcccgg cttcgtgggt gaagacggcc tcgccgacga
      781 tcggcttgtt ggcggccacc ggccggccgg atgcgccggc gaccagcgcg gatatggatg
      841 gaaattgcgc tggatccacg cctgtagcgt ggttgtacag gtggcgcaat gccatgacca
      901 cttcttccag cggtgcatta ccggcacgtt cgcccaggcc gttgaccgtg gtgttgacgt
      961 ggctcgcccc ggccttgacg gcggccagcg tgttggcagt ggccagcccg agatcgtcgt
     1021 gggcatgcat ttcaagttcg aggtcggtgg ccgcgtggag tgtacgaata gtgtggtagg
     1081 cgacgaatgg ttcgagcaga ccgagcgtgt cggcatagcg cagacgacat gcgcccccca
     1141 gttgggccgc ctcggcaacc tggcacagga agtccgcatc ggcgcgcgaa gcatcctcca
     1201 tgccgacgca tacctcgaac ccggcgtcgc gtgcctgggg caccagccag cggatacgct
     1261 caatcaccca gttacggtcg cggccgagct tgtggcggat ctggatgtcc gataccggga
     1321 tcgagagatt gatacggtcg atgcggcagc gtctggcggc ggcaaggtcg tctgcgcaca
     1381 tgcgtcccca taccatgagt ctggtggaca gacgcaacgc cgcaagcgcc tggatgtgtt
     1441 cgatctcgtt gtcacccatc gctggaatgc cgatttccat ttctggcaca ccggcctggt
     1501 cgagcgcacg agcgatggca atcttttcgc tggcgttgaa ggccacgccg gcggtttgtt
     1561 cgccgtcgcg taaagtggtg tcgttgatga tgaccatgat tcgatctcgc tgcaggcacg
     1621 ttgtgtttgt gttacagcaa gcgtcgtgcc agattttttt attcttttta ttcattttgt
     1681 tatgggttgt ctgtgtggcg tgcggccgat gtgaagcgga taacggtgtc gattttgtcg
     1741 gctttcacac agcagccgca gcggtatgcg ccggtgggtg ttggtttatg catcggtgac
     1801 atcaacgggt cggtggtcgt ccaggcggca gccgccctcc cagtacaacg cgggttcact
     1861 gatccgaaac agggtcatga tgaaatgctg tgttcatacg cggaaacttt ccccgcagcc
     1921 gcactggtcc ttgacattcg ggttgttgaa acggaagcct tcgttgagtc cctcgcgcgc
     1981 gaaatcgagt tctgttccgg cgagaaaggc atggcttttc ggatccacca ggatggtgac
     2041 gccttggctt tcgaatacca tgtcgtcggc gccgatttcg tcggcgaatt cgatcgcgta
     2101 ggccagcccg gagcaaccgc tggttttgac cgccagcctg agtccggcac ccttgccgcg
     2161 tttttcgatg aaggcgagta tgcggcgtgc cgcgttgggc gtcacggtga tgctcatgtc
     2221 ggcctccgtc gggttacatg gaaaacgagc tgccgcagcc gcaggtcgag gcggcattgg
     2281 gattgcgtac caggaagcgc gacccttcga gcccgtcttc gaaatccacc tctgcgccgg
     2341 ccagatactg caggctgacc atatcgacca gcacggtgat gccggcccgg tcgatccggg
     2401 tgtcatcgtc gttgaccgtt tcgtcgaaag caaaaccgta ctggaagccg gagcatccgc
     2461 cgccactgac ataaatgcgc agcttgagca gcggattgcc gtcttccacg atcatggtcc
     2521 tgactttgtc tgcggcggcg tcagtgaaat gcaacggggt gggcatcatg acctggatgc
     2581 cgtctgaagt ttccagcgtg ggggtgtcga gcagttcttc gagcatgatg ttctcctttc
     2641 agattcagtc agattcaggg agcgggggga gggcagccgg cgcacctgcc ctgccggtgc
     2701 cgcttgcgtc gtcatcggcc atgaacgagc caatgttgcg cgggtgttca taaggctcga
     2761 tcagagcatc gctgcaggcc atgccatgtt cctcgtcaat gtgttcctca atgcgcacgc
     2821 acgatgcgtt accagccatg ccgcaaggag cgtgccagaa taaatttcca ggttattcat
     2881 ggtgttgata cgttgcccac gatctgaccc ttgtcaggtt tgttgtggtc catacatttt
     2941 cagctgtcgt ggcgcctgtc ggtcagcgga tttttgccct gtcggcggcg ctgtaattgc
     3001 tgaaatggcc ttcgcgtttc gcctggctgc gtgctgcgcg gatgatggct gcggattttc
     3061 ccgcagcagc agggagtgaa acgcgtttgt ccagggcaat gccgccacag tcaggacaga
     3121 caggctgtgc accggagcgg atcaataatt cgcagctttt gccacaattg cgacattggt
     3181 attcaaatat gggcatggtg tgtttcctcg gccgaagtca tgggacagtg aatgcgctac
     3241 ccagcagcaa tatcgatgcc acgaaaataa catcatgaaa aaaaacatgt tttgattcga
     3301 tgtcatgtgt tctgcaccgc ttgttgtgtt gaatttgtcg caatacgtgc caccaggtcg
     3361 tggctggtgc gaatcttgct gactgttcgt acagggggtt cggccgggca gcatgactgt
     3421 ctggcagagc gcgacccggc acgcgtcggg cgtcacctgc ttcgcgcata cgaaaaacaa
     3481 ttcacgaggg tcggttcatg atgtgttgtc agagggaatt gcaatgggcg tgcaagcgac
     3541 cggaaaggtc agaatcgaag cggcgaccca ggaccgggaa ctgctgacga tctatgaagt
     3601 cagcaagata ctcggttcgt cgctgaattt gtccagaacg ttgcgtgacg ttctgaatgt
     3661 actgtcgttg taccttggca ttcgccgcgg catggcgagc ctggtgcagg aatcgggcga
     3721 actcaggctg gttgccgcct gggggctgac gcgcgaagag tttcatcgcg gacgattcgc
     3781 ggtgggcgag ggcattaccg gaagaatcct gcaaaccggc atgccggtcg tgttgcacga
     3841 tatcgccagc gaaccggttt tcctcaatcg caccgggtcg ctcgatgatc atggcgacga
     3901 gccgatctcg ttcgttggtg tgccggtcaa ggccggtacg caggtgatcg gagtattgag
     3961 cgtggatcat ccggtcgacc agcaggtcat gtctttccag aaggaagtca tgctgctcag
     4021 catggtggcg catctggtgg cccaggcctg gcgcctgcag cagggcgtgg ccgcagaacg
     4081 cgatgtgctg attcaggagc agcgccggct caagaccgaa ctgcgcggca aatacagcct
     4141 ggataatgtg gttggacatt ccaagaccat gcaggaagtg tttgcgcagg tgcatcaggc
     4201 ggcgccgggc cgggcgacgg tgctgctgcg cggcgagagt ggtaccggca aggaagtcat
     4261 tgcgcgttcg gtccatttcc tcagtgcgcg caaggatggg ccgtttatca agctcaactg
     4321 tgcggcgctg acggaaagcc tgctcgaatc cgaactgttc ggtcacgaaa agggcgcatt
     4381 taccggggca cagggtgagc gcaaggggcg tttcgagctg gcgcacggcg gtacgctgtt
     4441 ccttgacgaa attggtgaca tctcgccggc attccagacc aagctgctgc gtgtgctgca
     4501 ggaacgcgaa ttcgagcgcg tgggcggcac acgcccgatc cgtgtcgatg tgcgcctgat
     4561 cacggcgacc aaccgtaatc tggaaaaagc cgtgatggac ggcagcttcc gtgcagacct
     4621 gtattaccgc atcaacgtgg tcagcctgtt tttgccaccg ctgcgtgaac ggcgcgagga
     4681 cattccgtat ctggtcgaaa cgtttcttgc ccgtttcaat cgggaaaatc agcgtgagct
     4741 gaccatcgga gcggaagcaa tgcgcgtgct gctcaactgt tattggccgg gcaatgtgcg
     4801 cgaactggag aattgcgtcg aacgcactgc gaccatgact cgcggcagcg tgatccgtga
     4861 gatcgatttt ccttgtcagc atggccgctg tctgacgcaa atgctgcatg gcgaaggcga
     4921 ggctgcggtg gccgtgcagg tgatgcggcc ggtacgcgag cctgcgcccc aggttccggc
     4981 gcacggagcg ggcgatggcg cggtgccgcc atccagtgaa cgtgaacgcc tggtgtgggc
     5041 gctggaacaa tgtggctggg tgcaggccaa ggcggctcgc ctgctcaata tttcggcacg
     5101 gcagatgggg tatgcattga tgaagtacaa catcgaggtg cgcaagttct gagtcgcgcc
     5161 agccgggcag ctcaggctgc tgttcatttg tgatgggagt agaatcgggc agtgcgtttc
     5221 gatcccgtga ctgactttct ggagaatgaa catgaacacc tcgcgtgctg cgcccctgtt
     5281 ttatctctgt ctggttctgg tcctgctggc tgtcggtgtt ccggcgcgag ccgaatcgcg
     5341 tgccgacctc gatcgcgcgt cgcaaggcgc gctggaccgt ctctaccgca tcaatcctgc
     5401 cgcgcgggcc atcggcaacg cagcgcacgc ggtactggtg tttcccaaca tcgtcaaggc
     5461 cgggttggtg ttcggcggca gctacggcga aggtgagctg atcgtgaatg ggcatgtcga
     5521 cggatactac aattcggtct cggcatcatg ggggttgcag gcgggtgccc agacttacgg
     5581 ctacgccgtg ttcctcatga atgacaatgc ggtgaactac ctgcaccaga ctcatggctg
     5641 ggaaatcggt gtcgggccaa cggtggtgct ggtcaatgac ggcattgcac gcaacctgtc
     5701 cacgtctacg ctcaaggacg acgcttatgc cttcattttc ggacagcagg ggctgatggc
     5761 cgggctcaac atcgagggca ccaaggtgac tcgcatccag ccctgagtaa cgctctgtcc
     5821 gctgtgtgtc gcatgtacga caaacggtcg tatgtctgct gggtttgtta cgttcaacag
     5881 acgagtgtct ttcttagttc tatgattatc tggtgatttt tttctggcat ggttctggct
     5941 taggttggat tgtgaagcaa tttcgattgc ataatctgac aaggagccgg acatgcgggc
     6001 aacactggga aatcaggcgg atgcatcggg gcacgcgctc gggcaactgc tggacaagat
     6061 tgccgggcac aagggatgtg gtacttcggg tggtgagggg aaggccagct gcggctcgtc
     6121 cgccggaccg gccgacatgg cgcccgaagt ctgggaaaag gtcaagaatc acccttgcta
     6181 cagcgaagaa gcgcaccatc attacgctcg catgcatgtg gccgtggcgc cagcctgcaa
     6241 tatccagtgc aattactgca atcgcaaata cgattgtgcc aatgaatcgc gacccggcgt
     6301 ggtcagcgag aaactgaccc cggaacaggc agccaaaaaa gtgcttgcgg tggcctcggc
     6361 cattccacag atgaccgtgc tgggtattgc cgggccgggt gatgcgctgg ccaacccggt
     6421 caaaaccttc aggacattcg aactgatcgc caaaacggca cccgacatca agttgtgcct
     6481 gtccaccaat ggtctggcgc tgccggatta tgtggatacc atcaaagcgt tcaacgtcga
     6541 tcatgtcacg atcaccatca acatggtcga tcccgaggtc gggcagcaca tttatccgtg
     6601 gattttttac aggaacaagc gctacaccgg cattgacgca gcgcgcatcc tgtcggagcg
     6661 ccagttgctc ggtctggaga tgctgaccgc cgccggcgtg ctcaccaagg tcaactcggt
     6721 gatgatcccc ggtatcaacg accggcatct ggtggacgtc aaccgggccg tcaagtcacg
     6781 cggcgctttc ctgcacaaca tcatgccgct gatttcggcc gccgaacacg gtacggtgtt
     6841 cgggttgaac ggccagcgag gacccaccgc gcaggaactc aagcagctgc aggacgagtg
     6901 cgaaggcgag atgaacatga tgcgtcactg ccgccagtgt cgcgcagatg cggtcgggtt
     6961 gctgggtgaa gaccgcagcg ccgaattcac cagtgacaag atcgatgcca tgggtgaagt
     7021 ggtgtatgac cttgatacgc gcaagcaata tcaggaattg gtcgaggtgg agcggcagga
     7081 caaggtggct gcgcgcgaat cggctctgca ggagatcgat gcgaccgata ttcgcattct
     7141 tgtcgctgtg gccaccacag ggcaggggcg catcaacgaa catttcggcc acgcacacga
     7201 attccagatt tatgaagtgg gctcgggcgg cgccaaattc gtcgggcacc gtcgcgtcga
     7261 tctgtactgt cagggggggt atggcgagga agatacgctg ccatcagtgg tgcgcgccat
     7321 caatgactgt catgcggtat tcgtggcccg tatcggcggc tgcccgcgcg atgaactgca
     7381 agcggctggc atcgatccgg tcgatcgctt cgcgcatgag ttcatcgagg aatcggcaat
     7441 ccgctatttc ggagagtatt tcgccaaagt ctgtgccgga gacatcgaac acaggccgcg
     7501 cggtgatgcg gacatccgtc agggcgcttt taccgcggcg gcgtgacgga agccggctgg
     7561 cccgggaggg tgcagccggc acagggcaat tccgcccggc gggattggcg gggcaatgca
     7621 aacacagagg atatgaatca tggcttacaa gatcatcgca tcgcagtgca caggctgttc
     7681 agcctgcgaa cctgaatgtc ccaacgtcgc catacgcgag aaaaatgggg tgttcatcat
     7741 cgacccgaag aaatgcaccg aatgtatcgg gcatttcgat gacgcgcaat gcgtggctgt
     7801 gtgcccggtg gacaacacct gtgtcatcga cacaggctat ccgcgttacc aggcctgacc
     7861 ggaaaaggag tatcgcgatg aatatcacca tcacccccag ggcagaacaa ttcatgcgcc
     7921 gcatcgtgcg attcggcggg cctgaaggcg caggatttcg cctcagcgtg tctccgggcg
     7981 gctgttccgg actcagttcc gtgttcagtg ccgaagctgc cccgctacct ggtgatgccg
     8041 tgttcgaccg ggacgggttg cggctgttcc tgccggcaga gagccgtctg ctgctcgaag
     8101 gcgccaccat cgatttcgtc gacaccatga ccgagtccgg cttgagtttc atcctgccac
     8161 atgggggatc gtgcggttgt tcgagtgcct ccgccggtgc gtcagacggg agggccgtgg
     8221 ttgatatttc cgctatccgg cgagcgtgag gttgcggggc ctgacgtgga cgcaaacgca
     8281 ctgtccggta gctcggctgg cctgacactc gatgcggagt ggatggcttc ggcctgtctc
     8341 ggcggtatgc tgccgcagga tgtggccgac gacctgcagc gggctgcgtt gctgtaccaa
     8401 cgcggcaatg acgctgaacc ggtgttgctg gatctctacc agcgtgcctg gcaccacccg
     8461 gcggtgcaca tcgcactgta ccggttctat ttttatcgcc accgcctgca ggatgcgctc
     8521 gatgttgctg ttcgctgcct gctcaaggtg gccggcgatc tcggcctgcc ggatgactgg
     8581 cgggccgtgc ggccggagca ggcgaaattt tccgggccgg aatacgagat cgtgccacgc
     8641 ttttacctgt acaccctcaa gggttgtgcg tatctcaaca tgcgacttgg tcggttgcag
     8701 accggtgggg aaatgctggc gcagctccgc cggctcgatc cgcgcgaccg tcttggcgga
     8761 tcggtgctgg ccgaagtgct ggcgcgtatc ggggagagcg atgatgactg aacgcgacgt
     8821 tgcgggagac gcctttcccg ccgtggactg gcgagggctg ccgctggact gtgccgcttg
     8881 tgagcatcgt gacctgcgca gctccggcgg gtgccggcct ggttcggcgt gcgtacatga
     8941 ccggtatgca ttgcgtatcg acagattttt tcgctggaat ccgccgttgg cacgctatta
     9001 cctcgcccac ccgtattttg aggtacgcgc catcgtggcc aagcatgttg aggtgttctg
     9061 tctgccacag ctgatgacgg atgcagacga gaccgtgcgc tggagtgcag tgcaccggct
     9121 tacaccacgg cagttgttgc gcctgcgcga cgatccggac cgtgaggtgc gtattcgtgt
     9181 ggcggcccgg ctgcctgcgg tgcacctgat gccgatgatg gccgatgagg attatttcgt
     9241 ccgtagtatc gttgcgcgcc ggttaccgct ctcatggctg ccggacatga ggcacgatcg
     9301 tgacgccgaa gtgcgcgtcg aggtcgcacg ccggattgcg ccggaatggt tggcgatgct
     9361 ggccaccgac gcggctgtgg cggtccggct ggtggtggtg cgccgtctgc cggcgtggca
     9421 actgggcctg atgttgcacg atgcagactg gcgcgtgcgt tatgaggttg cgcggcgggt
     9481 cgaaactgga aaactggggg cactgtcggc tgacgaggac gatgccgtgc gcgagatggt
     9541 tcggttgcgt gtgcatgata tccgcgcgat tccgttgcgt gtcgcaggag gtgaggcatg
     9601 aagattagtc gtgacagcaa catcgtcgag ttgaacgatc caccggcatt tgattatggc
     9661 gaaaaggtca gaagcctgaa aaacgtgcgt aacgacggta cgtttcccgg cagggagatc
     9721 ggtgacgtcc tggtacgtaa gggcgatgtc ggctatgtct ccagtatcgg cacctttctg
     9781 caacagttct atatctatgg cgtggaattc atcgagtacg gttatcgcgt gggcatgcgg
     9841 gccagggagc tcgagtcgct cgacgaacct ggtgacggcg atgcacgcca cagggaatga
     9901 cggattcaag aggggggcag atgaaggcta tccgcgaagc gaaataccag tgggggcaac
     9961 gcgtgctcgc cgggatcgac ctggtgaacg atgggtcgca ccccgagtgc gcagccgatg
    10021 ggttgctggt ggcatgcggc acgccgggcg aggtcgtcca gatcggacat cacgtcgaag
    10081 ccaacgtgcc cgtgtacatg gtggagtttg cttctcctgg ccgacctgca tgcgtggtcg
    10141 gctgcatgga ggaggaactc agcctgcccg attgaagcgg aggatagcga tcatgttcgc
    10201 accagaggac gattgcccgc cactgcatcc gaacgagatc gatcggcacc gcatcgcccg
    10261 cctgctggca acgcggcagc gctaccgcta tgtgatgccg caggtgctgg cgacggaaag
    10321 tggctactgt gtggtcagcc cgtgctgttc gcgcaacatc gacgccagtg gcggcacgat
    10381 cgatatcgcg cgcctcgaat atctgccctg tgggaagggc tggttgctgt accgcaaaga
    10441 ccattccacg cacagatggc aggtgcatgt gcgctgtgct gatctggcag cggtgctggc
    10501 ttgcctgaat gcagatccgc aacgggtatt ctggcaatga atacccgtat ttatgccgac
    10561 aataatgcca cgactgcact ggcgccggaa gtgttgcagg ccgtgagcct cagcctgggc
    10621 gaactgtatg gcaatccatc cagtaagcac cgtgtcggtg atgctgcacg tgctgcggtg
    10681 acagctgcgc gggttgcggt ggcgatgctc attggcgcgc agcctgccga aatcgtgttc
    10741 acttcaggtg ctaccgagag caaccatctg gcgatcactg ctgcgctggc gcgcgagcca
    10801 gcgcgacggc atgtggtggt cagtgccgtg gagcacccat cgacgctgat gttgctggat
    10861 cggctggaac aggaaggcgt acgtgtgagc cggatcgccg tcgacgcata tggtctgccg
    10921 gatctggatc agttgcgcgc cgttgttacg ccgcagaccg cattggtgag catgatgtgg
    10981 gtcaacaacg agaccggggt gatcctgccg gtgcaggctg cggccaccat tgcagccgcg
    11041 cagggggtgc cgttccacag cgatgcagtg caggctgtgg ggcggctgcc gttgcacgtc
    11101 ggggacagcg gctgcgatct gttgtcgttc tccggacaca agttgcacgc gcccaaaggc
    11161 atcggcgtgc tgtgggtgcg caaggggttt gtactctcgt ccctgctggc cggacatcag
    11221 gaacgtaacc ggcgtggcgg gactgaaaat gtcccggcca tcgtcggtct cggcgtggca
    11281 gcgacactgg ctgcgcaacg tctggatcag gatgcggtcc acatcgctcg ggtgcgcgac
    11341 ctgctggagt ctgcgttgca aggcgaagcg gtcagcgtca acgggggcgg agtgccccgc
    11401 gtcgccaata ccagcaatct gcagttttct gctgtggaag caggcagcct gatcgaacgg
    11461 ctcgaccgac agggaatctg cgtatcgggc ggcgcggcgt gcactgcgcg tggcacacag
    11521 gcttcgcatg tgctgcgggc catgggacaa agcgagcagc aggcattgtc ggcggtgcgt
    11581 ttttccttct cacgctacag cacggcggaa gaagcgcagg ccattgccat ggccgtgcgg
    11641 gagctgatgc agcccgggtt ggcccgggtc gtctgatatc aacgatacga cagggagcag
    11701 gcatgaaagt gatgatacgc aggaattcgg ccggagcact ctcggtgtat gtgccgaaga
    11761 aagatctcga ggaaccggtg gtggcaatgg aacaccccgg aatatggggc ggcagcgtca
    11821 cgctggccaa tggctggaaa ctggagttgc cacacatgcc cgatggcaca gccctgccga
    11881 ttaccgtgga cgcgcgcaag accggagatt gagatgggca tcgaaaccgc acagcttgac
    11941 cagttaatgc tgctgctcga cagcctggcc gacgacacca gtccgctggc cggagtgcga
    12001 caggcctttc cgcattgggt ggtgtcgcaa tgcgatgcga ccgacctgcg cgacgagcag
    12061 ccagcgtgtt catcgcaccg ctatgacgtg catctggtcg atagccgtga gcaatgctgg
    12121 cgcatgaccg acgtggccgg gcaggcgacc ggagtgattc tggcgcggcg ggagtcggtg
    12181 tgatggttgc gactgacagt gaatggattg tcctcagcga tccggatatc agccagaacg
    12241 aactggaatc cgtagcctgc gtgctgcgct catcgcggtt gtcgtcgggc gaatgggtgg
    12301 aacggttcga agacacgttt gcggaatatc tgaaccgacg ttacgcggtg gcggtgtcca
    12361 gcggcacggc cggactgctg atcagcctgc gcgcactggg tatcggcccg ggtgacgagg
    12421 tgatcgccag cgcgtatggc tggcaccaga ttgcgcatgc catcgctctg accggcgcga
    12481 cgccggtatt cgccgatatc gattactggt cggggaccct ggccccggac aagacggagt
    12541 tgaaaatcac tccgcgtacc cgggccattc tggcgggcaa cagcaatgga catccggcag
    12601 catggcaacc gctgcgcgaa ctggcgcagc agcacggcgt ggcgctgatc gaagattcca
    12661 ccgaagccgt gggttcgcgt tatcgcggtg cgctgaccgg atcgttcggt gacgtggcgg
    12721 tgttcgattt ttcgcagccg tcagcattgg tctgcggtga aggcggcatg atcgtcaccg
    12781 acgatcctga cctggcgagc gaactgcgtt attaccgtgg tcgcagtctg gaggagcggt
    12841 tttcgatctc gattgccagc cggttgcctt tccagtcagg catgagcgat ctgactgcgg
    12901 cgctgggcct gacgcagctt gaacgcatcg acgagatact ggcacggcgc aagcgcaccg
    12961 agatgtggta ccagcgccat atcctgtctt tcgagggcat caagccggcc tatgtggcgc
    13021 ccgaagtcga cgaggtgcac tggatggtct atctggtaca tctcgggacg cgcttcacgc
    13081 gcagtgcacg caaccagatc gtcgatgatc ttcagaccgc gcgcatcgag gcgccggcgt
    13141 attgcaatcc gttgcatcag cagtattact attcggcgct cggatatcgg cgcggcgacc
    13201 tgtatgtaac ggaaaagctc gctgaccgtg ccctcgcgct gccgtttcac gcccatctcg
    13261 acgaagacac catcgctttc attgtgcaga cggccaagga ttcatcgatc aatgtcggcg
    13321 ccggcgcagc aatttatctt tgatcttacg caactacaga gcgggaacca catcatgtcc
    13381 agtctgaccg aacttttcca ggccctgcag caggagcgca tcgtgccgta tctcggcccg
    13441 gggatattgg gcctcaatgg cggtgatgcc gtcgtgccgg ccacgccgga agccttggca
    13501 gcttatcttt ccgccagggt gaccgtgccg tacaaaatac gcaaccggct caccgccgct
    13561 gcgcaataca tcgaaaattt caagcatcgc aagacgctgg tcacccacat gggcggggca
    13621 ttccttgcgc agccggacgc agaggcgttg cgccgtctgc tgaccgggct gtcgttgccg
    13681 ccgctcatcg tttcggtctg gtatgacgat accgtgctgc gcgcgctggc tgcgcacggc
    13741 gactggggca tggtgcaggg ctggtcgcaa gccgaacatt ttggcgagtg gtacggatat
    13801 taccgacctg acggcacgcc agcggatgaa gcgcaggcgc gtcaatggag catgctggtc
    13861 tatcagccgg tcggttgcgt gcggcctgtg ggaaattacc tggtatcgga ttcggattat
    13921 gtcgaggtgc tcacggaaat tgacatccag acgccgattc cgccacgggt acagcagttg
    13981 cgcagcgggc gcgagttcct gttcatcgga tgccgtttca gcgaccagtt gtcacgcagc
    14041 ttcgcacggc agatcatgaa gcgttccggc gaacggcact ggatgctgct caccgagacg
    14101 ccaacacgca acgaactgcg ttttttgcag gagcagcata tcagggtgat ccccatgacg
    14161 ctgcaggact ttgccagcca gctggtcagg gcgcaatccg aaccggcggc agcctgatgc
    14221 cgcccgagta tgcggcttcg gccgtttttt aaccataggg agaattccga catgtccatg
    14281 cttaccatcc agccgtccgg ccagaccatc gacgtgccag caggcacgac cctgctggcc
    14341 gccattctgc agtccggcgt cagtctgggg cataaatgcg acggcaaagc cgagtgcggt
    14401 tcctgccacc tgtatgtgct ggaagggcgc aagtcgctat cgcgcaccca gcgtgaggaa
    14461 aataacaagc tcgacaccat cgtcggcgtc agttcaaaat cccgcctggc ctgtcaggca
    14521 aaaatcggcg acgagcccgt caccatcgaa ctgctctctt tcgtctgact gcggcgcgcc
    14581 cgctggcccg ttgcgccgcg ttgcttggct tcctcagcgc agcgcggtac catccggggt
    14641 tgatcgccgg cacctgccgg agtcggaccg ggaggacgtg tggaacacca gaacagcgga
    14701 ttgcgtgcag ccggcgaccc ggcacagccg gacgtgccgc agcgtgcgct gccctggtgg
    14761 cgggtctcgc cacgctcgtt cctgcaactg attcttatcg gattttcatt ggtggccatg
    14821 ccgttgatcg cggtgctgat ccacagcgca gtggcgctgg atcgcctggc cgcagaaagt
    14881 cgcattgcgg tgcgccagtc ggtaagcatc gcgcatgcga gccgggaact cgccgacgat
    14941 gcgctgaaca tggaacgtgc ggcgcgccag agtgcgattc tcggtgaccc ggccttgctg
    15001 gaagcctatt acaacgcaca tgcgcagttc gaacggtcgc tggcggaact gtcgcgcttc
    15061 gatctcgatg ccgggcagcg cgcgcagctc gcccagttgc agtacgaaga agaccagctc
    15121 tatcagaggg tgaaagccgc gcaggggaca ggcgcagcgt tgctggcgca gggggccgga
    15181 ttttcatcgc tgctcgatgc cgcggacgct ttttcggcca atgcttcgcg gctcatcgaa
    15241 cagcgcgtcg atgcattgca ggccaccgcc aaccatgacc gggaggtggt gttctggcag
    15301 gtgctggcac tggtgcctgt ggtgctggcg ctggcggccg ccttttcgcg ccagatcgcg
    15361 cgcccgatcc gtcagatcga tctggccatc caccgcatgg ggcagggcaa gctcgccaac
    15421 ccggtcaccg tgagcggccc gcaggacctg cgcatgctcg gtgcgcgtct cgactggctg
    15481 cgtcgtcgcc tgctcggtct cgaagcgcaa aataccgaat tcctgcaaca tgtgtcgcat
    15541 gaactgaaaa caccgctgac agctatccgt gaaggctccg atctgctatc ttcgggagtg
    15601 gctggcgcgt tgtcgccgca gcaggcgcgg attgccggta tcctgttcga taatagcctg
    15661 cgcctgcaac ggcagatcga ggaattgctt cagtacagtg ccctgcaaaa cgagaaatcc
    15721 gccctggtca aggtgaaaag ccggctggtg cccctggtag agcaggtggt cgatgcctgg
    15781 caactggcga tcatgaacca gtcgctgcat gtggaacggc gtgtgccgga tgtgatcgtc
    15841 gagtgcgatg aacagaagtt tcgcactatc gtggacaatc tgttgtcgaa cgcggttcgc
    15901 ttctcgccgc aacattccac gatcctgatt cagggcgagg tgaacgacgg cgtgctggaa
    15961 ctggtggtgg ctgacgaagg gccgggcgtg ggcgaggccg accggcagaa ggtgtttgag
    16021 cctttctatc aggccaaacg accaccgcat gccgctgcgg gtggaaccgg ccttggtctg
    16081 gccattgcac gcgagtatgc gctggcccat ggtggtgatt tgcaactgct gcaacacgaa
    16141 ggcagcggcg cgcgcttttg tctgcgcatg ccgctgaacg gaaatacaca tgtttgaatg
    16201 tttgcgacgc atcctcccga tcaccatgct cgtcggcctc ctttccggat gcgcgcatac
    16261 gccgtctgtg ccgtctgtgc cgtctgtacc cccgatggcg ccggtggaat gtccgccgcc
    16321 gcagccggcg gccttgcagc cggcgatcgc cgcgccgacg ccgctgcagg aactgttgcg
    16381 cgaatcggcg cagtggcgaa ccttgccggc ggcggcgttg ctggatcgtt acgagaaggc
    16441 ccgcgggcag gtgcaacagt ccgccaccga ggaaaaccgc ttgcgcatgg ccttgctgtg
    16501 gttgttgccg aataccacgt ttcatgattc ggcatcggcg tatgccacgt tgcgcgacct
    16561 gtcggccggg cgggtaacgg gcgatgccga actggacgcc tttgccgcgg ccctgctgct
    16621 ggtggcggat gagggccagc atacggcaga tgacctgacg caacggctgc acgagcagca
    16681 gaaacgcgcg gatgccctgc aggaaaaggt ggatgcgatt cgtaacatgg agcgggagct
    16741 gatgcgccgc atcagcccct agtttttccg ggaagcgtga cgaaatggcc aagccgctga
    16801 tttttctggt cgatgacgat gccgatctgc tggcattgct ggcaatgcgc ctggaggcgg
    16861 ccggctacca gactgaatcg tttccgagtg ccgaggctgc gctcaaccgg ctgcagcgcg
    16921 tgcgcgcgca actgttgatc actgacatgc agatgggtgg tatgagcggt atggaactgt
    16981 tcgagcaggt gagtcgcacc atgccgagtg tgccagtcat catgctgacc gcgttcggca
    17041 ccattccgga tgcggtggcc gctgtgcagc gcggcatggc gggctatctg cccaagccgt
    17101 tcgacagtag ggtgctgctg gaacaggtcg acaaggcgct acgctggtcg gccgccgatg
    17161 ctgacagtcg ttccgaacca gccgctgcct ggcgtgcttc gatcattacc cagagcgcga
    17221 aaatggaaga agtgatcgat aaagcgcagc gcgtggcgac cggcgatgcc agcgtgctga
    17281 tcgccggtga tagcggcact ggcaaggaac tgcttgccca ggcgattcat tctgccagtc
    17341 cgcgtgccac caggccgttc atcgccgtca actgcgctgc gataccggag ccgctgctgg
    17401 agtcggaact gttcgggcac gtcaagggcg ccttcagcgg cgcagtgcgt gaccataccg
    17461 gcctgtttct tgcggccaat ggcggcacgc tgtttctcga cgagatcggt gacatgccgc
    17521 tgctgctgca ggccaagctg ttgcgcgtgc tgcaggaacg ccaggtgcgg ccggtcggcg
    17581 cggcgcatac gctgccggtg gatgtgcggg tgatttcggc aacccatcgt gatctgcgtg
    17641 ccgccatcgc agcgggccaa ttccgtgaag acctgtatta tcgcctgcat gtagtgggcc
    17701 tgaccattcc ggcgctggct gaacggcgtg aggacattcc gttgctcgcc aactatttcc
    17761 tgcggcgcat ggctgaaaaa tatgcgcgtc cggtgcaggc gttttcgccg caggccatgg
    17821 aactgcttgt cggcgagaac tggccgggca atgtgcgcca actgatgaac gtggtcgaac
    17881 aatgcgtggc gctgtcttcc accccgctga ttccgctgca gctggtgcag gatgcgatgc
    17941 agaaggccag cacgcacatg atttcgctgg aagaggcgcg ctccggattc gagcgcgagt
    18001 atctggtccg ggtgctcaag attactgctg gtaacgtcag ccaggcagcc aaactggccg
    18061 gacgcaaccg gaccgaattc tacaagctgc tgcaccgcca cgccatcgat cccactgcgt
    18121 tcaaggcata atactgtcgc ctgcaggaga caacgctaac ccaatgaaat gcatcgatat
    18181 tgcattttat gtcgattatt ttgtcgttat ttggcgacaa aataatgata cttgcagagt
    18241 gaaggcggga taatgtaaat aaaatcaaaa tgatatggtt atggcgcaga aattgcttgt
    18301 ggccagttgc cgtcgcgctt cagtcctggg tcgcgagcgg aataataatt gatcagaatg
    18361 actggaatac cgccatgaat cagatgacag aactgtcctg cgaaatgctg tttgaaactg
    18421 ttcctcaggc catgcacgtt gcggccgacg acgtgttgca aagctttgag tgtcgggttc
    18481 atgcctttcg tcgcctcgac gaacagctgg cgtgcaatta tgacgccgcc atgcaggagc
    18541 ttgaacgttt tgaggtcagc gatgatgtgc tggcgctgaa cgaaagcgtg gcgcgggcga
    18601 ctcgtgctct cgaggcgctc gaagccttcg aatcgttgcg actgggcatg ctgctggatc
    18661 tggaacgcga ataaatggtt gctgctgggc gcgccgcatc acgttgtcgc tgaaagcccc
    18721 gcaccttgag tgatggtgtt gccggtggcg gggcgtccga cgcttcggtc agaccgggcg
    18781 attttcccgg atcctgaccg gatccatgat cttcagcgct tcatccatac tgatttccgg
    18841 ccatgtttca ttgaaacagg ccgccttgtc ggcaatggca ccaatcttgc cgccagtgtc
    18901 gagtctcttc agatcgctgc cttccatgcg cagcaggtcg agcaattcct tgtagacggt
    18961 cgattcgtcg agcggcaata cccagaatcg tgtcccgccg taaggaatgc ggaatttctt
    19021 tgagatatcc aggttgccgg caaaaaagaa atactgcccg tctgctgcaa gcgtttcatc
    19081 cagtatgtcg cacaacagcg gcagatgagc cagtgacagg gcgaatccgg caaggaatgg
    19141 aatggtggtg gttcctgcgt cggcgaccat catgacctga tcgagtttga tcgccggtgg
    19201 ccgtttttcg tcagccagtg tcggctggaa ctcaagcgca atttcgccgc ggaaaccgaa
    19261 acgttccgcc tttccggcgg gacccttgtg taccgggctg agcagccggc cttctgcatc
    19321 gaggcgggca aacggggtgg cgctgatatc ggtcattgct gttccttcag aagttgcgca
    19381 gaaaatctgc agtggcgtaa aaagtcgagt ggcggtcaag tgcaaccgtg tattgtcgtg
    19441 tgctcatgac tgcaccggca ccactggaat ccccagcggt gcaatccgcc ccagtgcggt
    19501 attgacacgc cggcgcgcac cttcgatggt ggggggcgcg gtgagcagac cgtttgcgcc
    19561 tgctgccagc gcttcgctca cccccggctg ggtggcattg gcgcagacca gcagaatttt
    19621 cgcctgtggc aactgcgcac gcaggcgtga gattgtggcg gttgcatcct gcgcgatgtg
    19681 gctggtgccg agcagcacca gatcgggcgg ccagtgctgt gccttgctga tggctgcgtc
    19741 cggacccgcg agaatatgcg tctcgtactc atcggccagg atgaactgca accccatgct
    19801 gtttacttca ttctggtcca caatgaatat gcgcctgttt tccactgcgc gctgactgtc
    19861 taccccgatc tgcatgtcac ctcctcaccg tattggacga cgtgatccca gcaattcgcg
    19921 ttccagaaat acgatgttgg catctttaat ttatttttct gtttttattc aatctattat
    19981 agctgttgtg tggttgatgc tcctgtcagg atgcaatcca tgcacttgta ttgtttcata
    20041 cagccagcgg cggtttttgt gcggtttgta gcagacccaa taccgcgagc gatgtgagtt
    20101 tgaatttaat aaaaatattt aatatcaata cgttggtgtt atttttttgt ctggcatgct
    20161 tgttgctgca gtggcgctgc accacgtgtt ttgccagacg agtgactcaa aacgaagaaa
    20221 gagaaggttt cctcaaggcg ctaagttggt cgcaacggtt tttggtaaac aacttgtacg
    20281 gatcgttcac tgataaggag taacaaaatg gcagctttga gacaaatcgc cttctacggc
    20341 aaaggtggca tcggcaaatc gaccacttca caaaacacgc tggccgcgct ggccgaaatg
    20401 gggcagaaga tcctgatcgt cgggtgtgat cccaaggcgg actccacccg tctgatcctg
    20461 cactccaagg cgcaggatac catcctgtcg ctggctgcag atgccggttc agtggaagac
    20521 ctcgaactcg aggacgtgat gaaaatcggt taccgcgaca ttcgttgtgt cgaatccggc
    20581 ggccccgagc ctggtgtcgg ctgcgctggc cgcggtgtga tcacctcgat caactttctt
    20641 gaagaagaag gcgcttacga cggcgtggac tatgtctcgt acgacgtgct gggcgacgtg
    20701 gtgtgtggcg gttttgccat gccgattcgt gaaaacaagg cgcaggaaat ctacatcgtc
    20761 atgtcgggtg agatgatggc catgtatgcg gccaacaaca tttccaaagg catcctgaaa
    20821 tatgccaatt cgggcggtgt gcgtctgggc gggctggtgt gcaatgaacg ccagaccgac
    20881 aaggagctcg agctggccga atcgcttgcc ggcaagctcg gtacccgtct gatccacttc
    20941 gttccccgtg acaacgtggt gcaacacgct gaactgcgtc gcatgaccgt gctcgagtac
    21001 gcaccggagt ccaagcaggc caacgagtac cgccaactgg cgcagaagat ccatgccaat
    21061 gcaggcaatg gcacgatccc gacgccgatc accatggacg aactggaaga cctgctgatg
    21121 gaacacggca tcatgaagcg ggtagacgag accatcgtcg gcaagacggc cgccgaactg
    21181 gcggctgctg cggcctgatc aggggtagcg gggggcgcgc ggcggcaggg gccgccgcgc
    21241 tgcaggagag ttgcgcacag cgtatcgcaa ggtggaggtg atccaccagc caggcgcagc
    21301 gacgcggttt gcagagctct tccgggagcg ggaaacggtc cttgtgacag cttgctaatg
    21361 tagccaggag aagaaaatga gtctgacagt cgaagatgtg aaacagcgca acgaatcctt
    21421 gatcaaggaa gtgctcgagg tgtatcccga gaagaccgcc aagaaacgcg ccaagcacct
    21481 gaacgtgtat gaatcgggca aaccggactg cgacgtcaag tcgaacatca agtccctgcc
    21541 cggggtcatg accatccgcg ggtgtgccta tgcaggttcc aagggcgtgg tgtgggggcc
    21601 gatcaaagac atgatccaca tcagccacgg cccggtgggt tgtggccagt actcctgggc
    21661 atcgcggcgc aattattaca ttggcgtcac aggcatcgac agttttggca ccatgcagtt
    21721 cacttccgat ttccaggaaa aggatatcgt gttcggcggt gacaagaagc tggaaaaaat
    21781 catcgatgaa attcaggaac tgttcccgct caacaagggg atctcggtgc agtccgagtg
    21841 cccgatcggt ctgatcggtg acgacatcga agcggtgtcg aagaaaaaat ccaaggaata
    21901 cgatggcaag actatcgtgc cggtgcgttg cgaaggattt cgtggcgtgt cgcagtcact
    21961 gggtcaccat atcgccaatg acgcggtgcg tgattgggtg tttgacaaat ccggcgacaa
    22021 gcatgtcgac tttgaaggca caccgtacga tgtggccatc atcggggatt acaacatcgg
    22081 cggtgatgcc tggtcttcac gcatcctgct ggaggaaatg ggcctgcgcg tgatcgcgca
    22141 gtggtcgggc gatggcacgc tcgccgaact ggagaacacg cccaaggcca agctgaacgt
    22201 gctgcactgc tatcgttcga tgaactacat ttcgcgccac atggaagaga aatatggcat
    22261 cccctgggtg gagtacaact tcttcggtcc gtccaagatc gaggccagcc tgcgtgaaat
    22321 cgccagccac ttcgacgacc ggatcaagga aggtgcggaa cgcgtcattg ccaaatatcg
    22381 cacactgacc gatgcggtca tcgagaagta ccgtccgcgt ctcgaaggta aaacggtgat
    22441 gctgttcgtc ggcggcctgc gtccgcggca cgtcatcggc gcttatgagg atctgggtat
    22501 ggaagtggtc ggcactggct atgaattcgg ccacaacgac gattatcaac gcacgaccca
    22561 ctacgtgaag gatggcacgc tgatctatga cgacgtgacc ggctacgaat tcgagaaatt
    22621 cgtcgagaaa gtgcagcctg atctggtcgg ttccggcatc aaggaaaagt acgtgttcca
    22681 gaagatgggt gtgccgttcc gtcagatgca ctcctgggat tattccgggc cgtatcacgg
    22741 ctacgatggt tttgccatct tcgcccgcga catggatatg gcgatcaaca gcccggtctg
    22801 gggactgacc cgcgccccgt ggagcaaata attggtgtgg gccggatacg gccggccctg
    22861 cccgatgcaa cagatcactg gagaaacaaa atgagtcaga gcgcagaaaa aatcatcgat
    22921 catgaaatgc tgttccgcca gccggaatac actgaaatgt tcgccagcaa gaagcagaat
    22981 ttcgaattcc cgtatccggc tgacaaggtg accgagattc gtgattggac caagacagct
    23041 gagtaccgtg aaaagaattt cgctcgcgag gcgctgacgg tcaatccagc caaggcatgt
    23101 cagccgctcg gtgccgtgtt tgccgcggtc ggatttgcca ataccttgcc gtttgtgcat
    23161 ggttcgcaag gctgtgtggc ctactaccgc tcgcacttct cccgtcactt caaggaaccg
    23221 acgtcgtgcg tgtcgtcgtc gatgaccgaa gacgcggctg tgttcggcgg attgaacaac
    23281 atgatcgatg gcctggccaa ctcgtacagc atgtacaagc cggaaatgat tgccgtcagc
    23341 accacctgta tggccgaggt gatcggtgac gatctgggcg cgttcatcac caccgcgaaa
    23401 caaaagggca gcgtgccgga agatttcgat gtgccgtttg cgcacacacc ggcatttgtc
    23461 ggcagtcaca tcacgggtta cgataatgcg ctcaagggcg tcctgaccca tttctggcac
    23521 ggcaaggaac gtgtcgccaa caatgccatc aatttcatcg gcggatttga tggctttgtc
    23581 gttggcaata tgccggagat ccgccgcatt ctcactctgt tcggagcgga atacaccgtg
    23641 ctgtgcgatc cgtccgatgt gtggaacacg cccagcgacg gcgaattccg catgtatgat
    23701 ggtggcacca ccaaggccga ggtcaaggcg gcactcaatg ccaaggccac gatcctgttc
    23761 cagggcatca gcaccgagaa gaccggcaag tacattgccg agaccgggca ggaagtggta
    23821 tcgctgcatc acccgctcgg gctggccgga accgaccagt tcctgatgga agtctcgcgc
    23881 ctgaccggca aaccgatccc agcgcagctg gagcgcgagc gcggccgact ggtcgatgca
    23941 atcgccgatt ccagtgccca cattcacggc aagaagttcg cgctctatgg cgatcctgat
    24001 ctcatgctgg gtctgaccgc cttcctgctc gaactcggtg ccgagccggt gcatgtgctg
    24061 tcgaccaacg gcgacgatca ctgggcagcg aaggtgcaga ccctgttcga tgcttcgccg
    24121 ttcggcaagg gctgtcacgt ctatgccaag aaagacctgt ggcacatgcg ttcgctgctg
    24181 ttcaccgagc cggtcgatct gctcatcggc aatacctatg gcaagtacct tgaacgcgat
    24241 tgcggtgtgc cgctgatccg gctcggcttc ccgatcttcg accggcatca tcatcaccgt
    24301 ttcccgatct ggggctatga aggttcactg cgtgttctgg tgaccatcct ggacgagatt
    24361 ttcgaggccc ttgatgcgaa caccatcgat actgcgaaaa ccgattacag ctacgacatc
    24421 attcgctagt gtaccgggcg aagcctgcgg gcttcgccca cacaacggga gcctggagat
    24481 ggccacgatc actttcgttg ccatgcggct tgcgcgacgc gtcgaggtca aggtggatcc
    24541 ggcgcgcgac gacaatctgc tcgaattggc ggtgcggcac cacgttccat tgccctgtga
    24601 ctgcaaacgt ggcgaatgtg ggcagtgcgc agtcaaggtg gcgcggttgc attcggccac
    24661 cggcgtggcc cggctgacgc cgcgcgagcg ttatttgctg ctgcaggcgg gcaagatctc
    24721 gttgcgccag ttccacgacg agcatctgcc cgatcatccg gcaatgtggc ggctcgcatg
    24781 tgaatatcgt atcgaggacg aagagatcat ggtggctttc tagtccgccg tcaggttgca
    24841 ggcggttgtg tgccggacag tgtatggcag gtcattcccg cgcaggcggg gcagtgaggt
    24901 gaaacatgtc ggctaagctc gcgctgaaag tgcagcaagt gctcaatgaa ccgggttgtg
    24961 agatcaacca ggccaagagc ggcaaggatc gcaagaaagg ttgcaccagg cagttggtgc
    25021 ccggagctgc agccggcggc tgcgccttcg atggcgccaa gattgcattg cagccactga
    25081 ccgatgtcgc gcatctggtg cacggtccga ttgcctgcga aggcaattca tgggacaacc
    25141 gcggcgcgta ttcatccgga tcgaaactgt atcgcaccgg tttcaccacc gatatcaacg
    25201 aactcgatgt gatctacgga ggcgagaaac gactgttccg ttcgatacgc gaaatcatcg
    25261 aaaaatatga tccgccggcg gtatttgtct atcagacatg cgtgacggcg ttgatcggtg
    25321 acgacatcga ggcggtatgc cgggcagcga cgcagaaatt cggcaagcca gtgattccgg
    25381 tcgatgctcc cggctttgcg ggcaacaaga atctcggcaa caagctggcc ggagaagcat
    25441 tgctcgatta cgtgatcggt acggccgagc cggagatcac aacgccttac gacatcaaca
    25501 tcattggcga atataacctg gccggcgaac tgtggcaggt gaaacccttg cttgacgaac
    25561 ttggcattcg cgtgctggcc tgtatcagcg gtgatgcgcg ctaccacgag gtcgcctgtt
    25621 cacatcgggc ccgagcggca atgatggtgt gctcgaaggc gatgatcaat attgcacgca
    25681 agatgcatga acgctatgga atcccttttt ttgagggttc gttttatggc gtatcggaca
    25741 tgtcggatgc gttgcgtcag attgcagggt tgttggtgca gcgcggtgcg gccgatgaat
    25801 tgctcgggcg cactgaactg ctgattcgtc gggaggaagc gcgtgcctgg tcgcggttga
    25861 ggcaattcac tcagcggtta cgcggcaagc gtgtgctgtt gatcaccggc ggggtcaagt
    25921 catggtcggt ggtggcggcg ctgcaggaag tcggcatgga aattgtcgga accagcacca
    25981 agaaatccac gcttgaagac aaggaaaaga tcaagacctt gatgggcgag gatgcgcacg
    26041 ccatcgacga catgaccccg cgagagattt acgcgctact gcgggatgcc cgggccgaca
    26101 tcatgctctc tggcgggcgt tcgcaattca ttgcgctcaa ggcgcgcgtg ccctggctcg
    26161 acatcaatca ggagaggcac catgcctatg cgggttacga aggcatggtg gaactggtgc
    26221 ggcagatcga tctggcgctg cacagtccga tctggcgcca ggtccggctg accgctccgt
    26281 gggaggtata gcacatggcg catgtgactg aatcgaaaaa atcctgcacc gtgaatccgc
    26341 tcaaaatgag ccagccgctc ggtgccagct acgcctatct gggcatgaac cgctgcatgc
    26401 cggtgatgca tgggtcgcaa ggctgtacgg cgttcgggtt ggtactgatg gtgcgccact
    26461 tccgcgaagc gattccgctg cagaccactg ccatgaacga ggccaccacg gtgctgggtg
    26521 gctcggacaa tatcgaaaag gccatcctga acatcaagca acgtgccaat ccacagttga
    26581 tcggcatctg ttcgaccgga ctgactgaaa ccaggggcga agatgtgatg ggcgatctga
    26641 agctgatccg ctcgcgccat ccagagctcg gcgaaacgcg cgtggtgtat tgttccacgc
    26701 cggattatgt tggcgcattc caggacggat gggcggcagc agtgctggca atgatccagg
    26761 cgctggttga agggccggct cccgtccgta tcgacaacca gatcaatgtg ctggccggca
    26821 gccacctgac cgtggccgat ctggaagagt tgcgcgagat gatcgaggca ttcggcctgt
    26881 acccggtgtt gctgccagac gtgtccgggt cgctcgatgg gcacgtgcca gaagagtttt
    26941 caccagccac gcttggcggt accacgttgc agcaggtcgc ggcattgtcg gcatccgtgc
    27001 tgacgctggc ggtgggtgag cagatgcgcg gctgcgccat agaacttgaa atgcgcaccg
    27061 gggtgggcta cgaggtcttc gaccgtctga ccggactggg agccagcgac cgattgctgg
    27121 ccacactgtc gcgtctgtcg gggcagccgg tgccgctgcg ctaccggcgt cagcgcagcc
    27181 agctggttga tgccatgctg gatgggcatt tcttttttgg gggcaaaaaa gtggccattg
    27241 gcgccgagcc ggacttgctg tggggcatga caaactggtt gcacgagatg gggtgcgaaa
    27301 ttgtcgcggc ggtcacgacg accagatcgc cgttgctggc ggacgtgccc accgaagaag
    27361 tgcttcttgg agatctggaa gacctggaag tgcgtgcagt cggctgtgat ctgttgttga
    27421 cgcattcgca tggccggcag atggccgaac ggctggcaat tccgttcctg cgcatcggtc
    27481 tgccgctgtt cgaccggctg ggcgccatgc atcggctcag tatcggctat cgcggcaccc
    27541 gtgacctggt gttcgaggtc ggcaacctgt tcatggccag cgaacaccgg atgcaggcgg
    27601 acagttggcc attaccggcg tcaagtcagg cacatgccag tgtgatcgct gagtaattta
    27661 ttgttttgtc aggagttata aatgaaagtg gcttttgcga cacaggatct gcaacgcatc
    27721 gatgcgcatt ttggctgggc gaagaatctt gcggtgtacg acgtcaccgc agaggggtac
    27781 agcttcgtgg agacctttgc ctttgatggc gatctgcagg aagacggtaa cgaggacaag
    27841 cttgcgccca agctggtagc cattggtgat tgcgccattc tgtacgtggc cgccattggc
    27901 ggctctgccg cagccagggt ggtggccagc cgtatccacc cggtcaaggt cgcgcagccg
    27961 gaaccgatca tggatgtgct cgacaggctg caggaagtgc ttcggggtac accgccgccg
    28021 tggatgcgca aggcactgat gaagggacag gcgcgatcga tggattttga agacgaggtg
    28081 tgagatgagc gaaagcgtat tggccgacac acagtcggtt tcggcgatgg attccccgtt
    28141 catgcgggaa ctggtcaagc aatggcgggc tcaggacacg cacggagcct gggatggaaa
    28201 aagtgatgct gatctgatcg cgccatatct cgtcaccgcc gaacagcgcc gggaaatgcc
    28261 cattattggt gaccctgacc ccgagatcct gtggcggctc gaactgtttt ataacgcggt
    28321 tggattgtcg atcgagcgac gcaccaaaat catggtgacg ccgatgatga aaatgtcgca
    28381 tgaggggttc gggcgactgg tgctattggc gggtcgactg gtggcagtga accgccagtt
    28441 gcgtgacgtg catcgttttg gttttgtcac tgtggaaaag ctggctgaag aaggtgaaaa
    28501 gctggtgcag gccggtgtgg acatgatcgg gcagtttccg gacgctgcca cttatggata
    28561 ggagtttcag gatggaaaac atcgaagcac tcaaggcgca gatcaaaaag ctgaaccagc
    28621 aggcaacggc gttgaaaatg gatttgcatg acctggccga agatttgccc accgggtggg
    28681 agcgcatcct cgatgtggcg cagaaaaccc atgcggcgca ttcggcgctc atggaagcgc
    28741 gagcgcggct ggccgatacg ggggggtgat gccggctccg gatgactcgg aatgacattc
    28801 agttggagac tgcgatggac gtattcaaga ttgttttgcc cagcggcgaa agctggaccc
    28861 cccatttcgt cggtgaattg aaccaggaca aatgtatcgg ttgcggacgc tgtttcaagg
    28921 tgtgctcgcg cgatgtgctg caactggtcg gtatcaatga agcgggggat gcggtgctga
    28981 ttgcggtggg tgccgacgat gacgatgacg aggagtacga gaagaaggtc atgactatcg
    29041 ctcatccgga aaactgcatc ggttgcgaag cctgctcgaa gatctgtccg aaaaaatgct
    29101 atacgcacgg tccgttgcgc ctcggataaa gggtattcga tcgtgaatca ccggctggaa
    29161 tggtgtctgg atcgcaactg gttgctggcc ggagcgcgta atccacgcga cctggcgaca
    29221 caggtgtttg ccggtgtgct gcactcgctc ggtgcaggcc ggatcgatcc gcccggatac
    29281 ggacctgggc tggacagtgc cgactgtctc gatctgttgc agcgccattt cccgttggtt
    29341 gcgctgccgg tgcatgcaag cctgtgcgcc gtggctggca acgccggtcg tcgtagcgaa
    29401 ttcgatgagc tggtacagct gctgctggaa tatcgtgccg acggcagtga gcagacgcgc
    29461 tgggtggcgc atgccgtcgc cagcggatgc ctgggcagtg atcacctgta tgctgacatg
    29521 gggctgccac agcgctcgca gctctcgcaa ctgctgcgtg agcatttccc cgcgctgtgt
    29581 ctgaagaatc cggactcggc aatgaaatgg aaaaagtttt tctaccgcca gttgtgtctg
    29641 ggcgcagaaa tcagcctgtg cccggcaccc ggctgccaga cctgcgtcga tcgccaggac
    29701 tgtttcgtca gtgcatgcgc ggacggcaca atcgacgcgc aggcggactg acatgcgcag
    29761 gaatggtttt tgctgtgttt gtcgttatgc aaatgcgttc ataactcatc atgtgttttg
    29821 cagtgttcgg ggaggtgtgc gatgtgggtc aacggtgcat gtaacggaat tgccgtcagg
    29881 gtggcgcggg cactgactgg cccggggagc ggcgcggtgg aaggatgcgc aggacgttcg
    29941 ggcacaggca ggtcacgggt ctggcttggc gggttgttgt ggctggtcgc cggcatggcg
    30001 tacggcgata gcctgaaagt ggcggtggcg gcaaatttca ctgcaccgat gcagaaaatc
    30061 gctgcgatat tcgaacagca gaccgggcag catctgactc tggtgtttgg cgggacgggc
    30121 aagttttatg cgcagatccg caatggtgcg cctttcgacg tgctgctggc ggctgatcag
    30181 gcgactgcgg aaaagctcgt gcagcaggga ctggcgcagc ctgacagccg ctttgtctat
    30241 gccactggca ggctggtgtt gtggagcgcc atgccgagac gggtcgatgc cgcaggaaac
    30301 gtattgcgca gtggcgactg gcaacatctt gcccttgcca atccgcagct tgcgccgtat
    30361 ggggcggcag ccatgcaggt gttggatcat atggggctga ctgcggcgct gcaaccgaaa
    30421 ctggtgcgcg gtgaagacat cggtcagacg tggcagtttg tcgccagtgg caatgccgac
    30481 ctcggatttg tcgcttattc gcaggtgctg cgcgatggca agccgctgcc gggttcgctg
    30541 tggctggtgc ccgcaaactg gtatgccccg attcgccagg aagccgtcgt gctggccgca
    30601 ggcaaaaaca acccggccgc cggtcagttg atgcgtttgc tgcgcagcga tgcaatcaga
    30661 gcgctgatcc gcagttatgg ttatggcacc tgaacaccat gctgccagtg ccgacatttg
    30721 acgctgaaga ctgggcagcc gttggcctga cggtacggct ggccacggtg accacgctgc
    30781 tgttgctgct gttgggaacg ccgctggcgt ggtggcttgc gcgatcgcgc agtcgactgg
    30841 cggcgctggt cggtacgctg gtgacgttgc cgctggtgtt gccgccttcg gtgctgggct
    30901 tctatctgct gctcatgctg gccccacagg ggccgctcgg acagttcgtg cacgagttgg
    30961 gcggggaagc aatgccattt acgttttccg ggctggtggc cgggtcgatg gtctacagtc
    31021 tgccgtttgt ggtgcagccg ttgcggactg ccctggcagg actgggcgag cgtccgttcg
    31081 aggttgcggc gacgttgcgc gctacgccgc tcgaccggtt tttcagcgtg gcgttgcctt
    31141 cggcgcgcca gggcgtcctt aatgccaccg tgctcggttt tgcccacacc gttggcgagt
    31201 ttggtgtggt gttgatgatt ggcgggaata tcccgggaag gacccaggtg ctgtcgctgc
    31261 gtatctatga gcatgtcgag gcgatggaat atgcccaggc cgggtggctc gccggcggca
    31321 tgctggcgtt ctccttcgtg gtgctgctgt tgttgcatgc gcgggggaaa cccgcgctgg
    31381 ccggatgatg gtgcacgata ctcgcgatat cgtggcatgc tttcgtctgc aacggcgcgg
    31441 cttcgggctg gatgtcgacc tgcggctgcc gggacgggga gtgagtgtgc tgtatggtgc
    31501 ttcggggtcc ggcaagacca cgctgctgcg ttgcatggcc gggctcgaac gtgccacggg
    31561 tcggtttgag attgccgcac aggtgtggca ggacgatcgg gtgcgccgct tcctgccgcc
    31621 gcatcgccgt gcgctcggct atgtgttcca ggaacccagc ctgtttgccc acctcgatgt
    31681 gttgcacaac attgaatatg gtctgcgtcg cagtgaacgg ccacatgatc gttcgcggct
    31741 cgatcaggcg gtggcgatgc tgggtatcgg cgcgttattg cgacgctggc cggcgcaact
    31801 gtctggcggc gaacagcagc gtgtcggcat ggcccgtgcg cttgccagct ggccgcgcct
    31861 gttgctgatg gatgagccac tggccgcgct cgatgaagcg cgcaagtcgg agatcctgcc
    31921 gtacctcgac acgttgcacc gtgagctggc catcccggtg gtctatgtca gccacgcagc
    31981 cgatgaggtg gcgcggttgg ccgatcatgt cgtgctgctt gacggcggtc gggtacgggc
    32041 aagcgggccg gtggccgaga tgttttcgcg caccgacctg gccttggcgc agcgtgacga
    32101 agcgggtgcg gtcatcgatg cgtgcgtgga aacgcatgat gccaggtggc atctgagtgg
    32161 cctgcgttgt cctgcgggtc tgttgtgggt gcggcagctg gaggtgccgc cgggcacgcc
    32221 cgtacgcgcg cgcatcgctg cacgggatgt cagtctggca ttgcaggggc cgcgcgatac
    32281 cagcatcctg aacagtctgc cggcccgggt gtgcagtctg gtggccgatg tacatccctc
    32341 gcaggtgctg gttcagctcg atgctgccgg ggtgctgttg ctggcgcgca tcactcgtcg
    32401 ttcgctggat gcgctggcac tggtgcccgg tatgcaggtg tgggcgcaag tgaagagcgt
    32461 ggctgtgccc aggtaaacgc ttccggcagc gatgctgccg gaagtcgccg ggcgttggct
    32521 gtcagaacga ataaccgagg cctacgcgcg caacggtgta atgggtggtg ctgtccggtt
    32581 cccaggccac atagccgcca cccacatcga agaggctgct ggcaccatat ttgaagctgg
    32641 tgtaatcgac gcccaggttg ccgtgcaggt gcgtggtgaa ggcatagtcg actgagccac
    32701 cgatgcgcca cagcgtcgag gtgccgagcg agccggagaa accgctatag ccgccgtcgg
    32761 gagccacggt gatgtttgcg ccgaaggtgc cgccgacgag gccgttcgcg gtcagggtca
    32821 ggttgtgcgt cggggcatac tgcgccaggg caccgaggcc gtagtagcgg ttgcggtagg
    32881 tttcgcccgc attgacgccg cgatcccagg agtggtagcc gagttcgaaa tacggcgtga
    32941 gcatgctgtt ctggctgagc gagaatccct tgccccagcg cagcgacagg tcttccagtg
    33001 tagcgccgtc ggaaccggaa aggctgccgt aggcattggt ggtgcctgca atcggctggc
    33061 caacgtaact ggtgtcgcca ttgctgtggc tgtagttcag gctcaggtag tcattgccga
    33121 gccaccagtc ctgcatggtg gacaggttga ctgcgtagcc ggaaatggtg ccgttttccg
    33181 tgtccagcgt gccggtcggc gtgcccagca gaccactgcc ggtttcggtg tagtcgacct
    33241 tggtcgaggt gtactgtatg ccgatctggt tgttggtgga caggattgcg gaggtgtctg
    33301 cctgggcgga cagcatcggg ctggcgcaca ggacggcgac ggccagcatg tgggattttg
    33361 tcatttattc tttcctttca ttggtgttat caggatggcc gcatgatagc gcaaattcgc
    33421 ttctgtcatg aagactgatc cgcatactgt tgtaaatatg acaatcagtg cgttgtccgg
    33481 cgcaggtaga gccagaagct ttcctcgtcg tcctgtgggc gcgtgccatg ccagaccagt
    33541 gcccatccgg gcggggtggc gggtgggcga agcccccact ggtccaccag ccgggccctg
    33601 caggccggat cgtcgctgtg ggtacgacgg atgccgcgcc tggcgaggta atagtcgaac
    33661 atcggccggg tcgattcgcc cagttgcagt ccggatacgc agttgtagtc ggccggcagt
    33721 gcctgtgctg cctgatcgaa aacttcgcgg aagcgtttgg cctggtcgac ccatggttgc
    33781 cagagcatgg cgaataccag ccagaatacg gtgacggcgc ttgcggtcga tagcaacgcc
    33841 cgtgcgtcga gacgtggcag gctgcgccag ctgcccagcc acagcagcgt gccggcgagc
    33901 gccagcgtga cagcgaccgg gtggaatggc atgacgaaat tcggatcgag cccgtgtgga
    33961 atgccccatc gcggtgggtg gccggtgcgg atcatgatgg cccagaccag ccaggaaaat
    34021 gcgacggccg gcgcgaacag cccgcgggcg agccagtcga tggcgcgttc cagtgtgcgc
    34081 ggcaggcacg tcgcggctgg aattgcgagc agatcgaggc cgatgagcag cggcagcccg
    34141 taggcggtac gcgctgaggc ggagacctgc attaccgtca ggtagactgc gaaggccaca
    34201 ctgccgatct gcaatccggg gtcgcgtccg aagcttgcgc gctgccgcca cagcgtcagc
    34261 acggcgagag gccacagcgg ccagccgaac catggcagtg tgcggggcca gaaccaggtc
    34321 gagctgtctg cgccgagttg cgcaacggaa aaaccgagaa agcggccaag gttgttgagc
    34381 cagaaccact ggtagaacag ttctggtgaa cgcagccaga gtgcaactgg ccagatgacg
    34441 agccagggca ataccgccag catggcgcag gcgatgacca gcggcaggcg gggggtgcgc
    34501 caggcggcga acagcagcgg caacaacact ccggtgacag caatggcgcc gggaaccagc
    34561 agtcctttgg caaggaaacc gatgccggcg ccgctgccga gccacagacc accttgcacg
    34621 gcatgggtgc ggctgcgcag cagcccgtac agggcgatgg tgtaaccggt cagcatcggc
    34681 acatcggtga tcatgatgtg gctttcgaac agcaggccga aacagccggt cagaccaagt
    34741 acggccaggc ggccactccc gggtccccag gcttcgcgcg cggcgagggc agtgaaagcg
    34801 agggtcagcg cgctgaacag tccactggcc agccgcgcgg cgtcgaatgc cggcagtatg
    34861 ccctgcaggg tgcgggccag caggctggcc acccagtagt agagcggcgg tttttccatg
    34921 aacggcacac cggcgttgag cggaaccacc cagtcgccac tggacagcat gtggcgtacg
    34981 ataccgaaga tgtaggtttc gtcctgcttc caggggtcgt gtccggtcag tcccggcagg
    35041 agccaggcca gcgccagcag tactacggcg gcaaccggca ccgggccgcg gggggcggat
    35101 tcaggcatgg ggggtgtcga gtggcatggc cagcgtgccg gaacggccag gcagggtgcc
    35161 catgccgacc cgcctgcgcg gcagggccgc gacatcgagt gcagcgtaca gtccggcgag
    35221 gctgaccaga cgatagccct gttgccgcca gccgctgagc agggcctcga aggccggggc
    35281 cagtttcatg ccttcgagtt ctgcgtgcag cgtgtagaca tgaccggtgg cgctttccgc
    35341 ttcggtcagt gcgagcacgc gcccggccac ggtgccggca tcgatgccgt ccagtccgat
    35401 cagttcgtcg agcgttggca gcgtggtcgg caactgtacg catgccgcag tgccatcggc
    35461 gagtaacggg acgaacggtg ccgggccgcg ggtgtcagag cagtacgcca tgccccagtc
    35521 ggtttcctga cggtaggcgc tgtcgttcat ctgccatcca gctgcgccgt gcgtgggcgg
    35581 atcggcattg aatacatcca catagctctc atgggcaagc tgcatttcct gccgggtcca
    35641 ctctggcgat ttgcgggcaa cgaaatcctg ccacttgatg tgatcccagg tgtggatgcc
    35701 ggtttcgaaa ccgtgcgcag ccacgtcgag caggatgttg cggccgcgtt gcgcgatatg
    35761 cgggccgggc agcagtgtgc cgtagagcag ggttttccag ccataatgtt cgaggacaga
    35821 ggtgcgcgac accttgctca tgaaaccggg gcgaaacacc cgtcggatag cgcggccagt
    35881 atggtccggc cccaggctga agagaaaggt ggcgtcggct gtgtgcttgc gcaggatgcg
    35941 caccaggttc ggcacgcctt cgagcgtgcc gcgccaggtg tcgacatcga ctttgagtgc
    36001 gagcagtcgc atggtgcgtt cgtgtttcct gtgccagctc agtccatcag cgcgcgggcc
    36061 gaggccactt gaccgcgata ggcatcgaac acggcctcca acgcctgggt catgctgaat
    36121 tcgggcttcc agtcgagctc gttcatggtg ttgtcgattt ttggcacgcg attctgcaca
    36181 tcctgatagc ctttgccata gtaggcgtcg gcagtggtct cgacgatgtt gacgtgcgct
    36241 gcgctttcgc ggtattccgg atacttgcgt gccaggtcga gcatcatgtg ggccaggtcg
    36301 cggatggagt gattgttggc cgggttgcca atgttgtaga tccgtcccga tgcgacgccg
    36361 ttgcgattct cgatgatttt catcatggcc gagatgccgt cagcaatata ggtgaaggcg
    36421 cgcttctggt gtccaccatc caccagcttg atgtcttcgc cgcgcacgat gtggccgagg
    36481 aactgggtga tgacgcgcga actgccttcc tttggggtgt gaatgctgtc caggccggcg
    36541 ccgatccagt tgaacgggcg gaacagcgtg aaatcgagct cacccttcat gccataggcc
    36601 cagattaccc ggtccatcag ctgcttgctg cacgaataga tccagcgtgg tttgttgatc
    36661 gggccgagaa tgagttcgga atgctccggg tcgaactgtt cgtcgtggca catgccatac
    36721 acttcggaag tggacgggaa caggatgcgc ttgcggtact tgacgcactg gcgcacgatg
    36781 ggcaggttgg cttcgaaatc gagttcgaac acgcgcagcg gatcgttgac ataggtcgcc
    36841 ggcgtggcga tcgcgaccag cggcagcacc acgtcgcact tcttgacgtg gtattcgatc
    36901 cattccttgt tgatggtgat gtcaccctcg aaaaagtgga aacgcgggtg gctggcgaat
    36961 tcctcgatct tgtcacgcat catgtccatg ccgtagattt cccagtcggt ggattcgagg
    37021 atgcgtttgg acagatggtg gccgataaaa ccgttgacac cgaggatgag gatttttttc
    37081 atttggctct cttcgtgggt aggtggtgga atcagtcaag cgcaatgggt tgcgctccgg
    37141 gagcgggttg ccagctgcgg ccatccagtt cggcgttgag cagttgcagc gtgccgcccc
    37201 caccgcaatg gacgaaacag ccgcgattat cgcaaaaaac ggcaggacgg gtatgcgggg
    37261 cgggtgaggt ggcatcggac acgcgactgc gcagcacgcg cagcgtctgt ccgcccagtt
    37321 cggtgaatgc gccgggatag ggcggggcca cagcccggat caggttgtgg atcgtcgttg
    37381 cagactgcgt ccagtcgatg cggccgtcct gcggccggcg gccgccgaaa tagctgccct
    37441 gcgccaggtc ctgcgggatg cgtggcgcgg taccggcgat cagttccggc agggcgcgat
    37501 acagcgtgag ttcggcggcg caggcgacct tgttgaaaac gtcgatggcc agatcgtcgc
    37561 gcaggatcgg cacgaccgtc tgggcaacga tatcgccggc atccggtttt tccgtcatgt
    37621 aatgcagtgt ggcgccggtt tcggtttcac catgcacgat ggcccagttg accggtacgc
    37681 gaccgcgata gcgcggcagc agcgaaccat gcaggttgag ggcgccgcgc gtcggtaatc
    37741 cgagcagcgg cgccttgagc atgttgcgat agtagaacga gaacaggaaa tcgggctgta
    37801 gcgcggcgat gtggtgccgt agcgcttcgg tgttcggatc gtcgggggtg atgcacggca
    37861 gatcattgcg cttggccagg cgctcgacgc tgtcgaacca gatgttttcg cccggagcgt
    37921 cgtggtgggt gaccacgagg gcgatgtcca cgccggcgtc gagcagcacc gaaaggcagc
    37981 gcacgccgac attatggtag gcaaatacca ctgcgcgcgt cacgaatgtg tctccgggcg
    38041 cgagagcagc gctgctgccg gcggctgttc gtccgggctc tgcaggatgg tatcgatgag
    38101 gtagcgtggc cgctggcgta cctgctggta gatgcggccg atgtactctc cgagcagacc
    38161 gatcccgaac agcagcacgc cgatgaggaa gaacaggatg ccgaacaggg tgaatacgcc
    38221 gtcgacttcc ggcccgagca atatccgtcg cagcgccagg tagatgacga atagcgccga
    38281 aagtagcgac agtccgacac cgaccaatga gaaaatctgc aacggcatga gagaaaagcc
    38341 ggtcacaagg tcgaaattta ggcggatgag cttgtagagc gaatatttcg attcgccggc
    38401 ggcgcgttcc tcgtgcgcca cctcgatttc gcccgggttg cgcgcatagg tgtatgcgag
    38461 tgccggtatg aaggtgttgg cttcctggct gtgattgatg gcctgaatga tgtcgcggct
    38521 gtaggcgcgc agcatgcatc cctggtcggt catcaccaca tgggtgatgc gctcgcgtat
    38581 acggttcatc atccatgacg catggcgacg ccaggagaca tcctgacgct ggcgacgcac
    38641 gccaccaaca taatcgtagc cttcgtccat cttttgcagc aggcggccga tttcctcggg
    38701 cggattctgc aggtcggcgt cgagcgttac gatccgctgg ccgcgacatt gcgcgaatgc
    38761 cgagaggatg gccatgtgct ggccgaaatt gcctgcaagc aggatcacgc gcgtcacgtc
    38821 gggacggcgc tggtactgct cgcgcagtat ggcggcggag cggtcgcggc tgccgtcgtt
    38881 gacgaacaat acctcatagg cgataccgag ggcgtcgagg gcagggtaca gtcgcgcaaa
    38941 cagcgcagcc agtccctgtt cttcgttgta gaccggtatg acgaccgaca gggccggagt
    39001 gttcatgaca gggcttcctg cagcgtgacg tgcagcgcat cgcagacgcg ctgcacgtcg
    39061 tggtcggaca tggcggggaa cagcggcagc gtgatggtgc ccgcgccgat gcgttcggcc
    39121 tggggaaatt cgccttcgtg gtgacccagc gcgcggtata ggtggaacag gtggatgggt
    39181 gggtagtgta ccccaagcgc gataccacgc gcgcgcatgc gggcgatgaa ttcgccgcgg
    39241 ccgaaacgca tgtctccgag cggcagcacg agctggaaca tgtgccagtt gctgtcgctg
    39301 aaattggcca gcggcagttc gaagcctgcg gccgggtcga ggcgggcaaa atacagtcgc
    39361 gccagtgcac ggcgccgcgc ggtgaattcg cccagatgtg gcagttggcc gaggccaatg
    39421 gcggcggcaa ggtcggtcag gttgaatttt ccaccgggca gatcgacttc catgctgccg
    39481 tccgccaggc gcaccacgcc ctgcaggcgc attttttcgc acgccggaac caggcttgca
    39541 tcgggcagga ccaggcagcc accttcaccg gtggtgatgt ttttgttggc gtggaaactg
    39601 aaagccacca ggtcgccgaa actgccgatc ggtttgccat gccagctgct gccgaaagat
    39661 tgtgccgcgt cctcgatcac gcgcagtcca tgcttccatg cgatgtcgta gagcgcgtcg
    39721 cggtccgccg gcaggccggc cagatcgacc ggtatgatgg cgcgggtgcg cggggtgatg
    39781 gcttctgcca cgcggcgcgg atcgatattg cgtgtgaccg gatcgatgtc gacgaataca
    39841 ggcgtggcgc cagcatgcag gatgacattg gccgttgcca cccaggacaa cggggtggta
    39901 atgacctcgt cgccaggctg gatgccgatg aggttgagcg cgatttcgag cgcggcggtg
    39961 ccggaattga gcgagcgaac cggccgtccg cccatgagtg ccgacagttc gccttcaaag
    40021 gcctgtactt tcgggccgct ggtcaaccag ccggaacgca gtacggccgt taccgattcg
    40081 atggtggctt cgtcgatcgc agggcgggta aatggcagga tgctgttcat gttcgtcatt
    40141 ccgtcatggc gcaggtcagg tgcgcgtgat gagaaaaacc cccagcatga tgaccaggat
    40201 tccgctgatt cgttcgatgc tgagcgtttc gccaaacagc caccacgaag ccagcgcggt
    40261 gacgatgtag ccgaaggata ccatcgggta ggccagactg acttcgacgc gcgacaacac
    40321 cagaatccac gccaccacgc tgacgacgta gcaggccagg ccgccgagaa tgtgcggttc
    40381 ggtggccagc ttccagccga tgggcaacac gtttgccatg ctgaaggcga attcgcccac
    40441 gctgcgcgtg ccggccttga gcagcagttg cgctgcggca ttgaggctga cgccggtgag
    40501 gatgagcagc cagctggtga ggttcatgag tgggttcctg tgtgcggcgt caccaccacg
    40561 gtgcgtcgcg tgtcgctggc gatgacctgc atggccaggc cgtccgcttg cagcttgcgg
    40621 tattcggccg gggacatgac cgccagtgcg taaggttgtc gcggccagat gcgttcgaat
    40681 tcggccacgg tggggatcca tttttgtggt tcctgctgaa tgccgaactg catttcgtcc
    40741 gggtgcgcga ccagggtcat cgtgcgttgc agatatggtg gcaaggtctg ttcgtacatg
    40801 gcgacgctgt agaatggcac gccgggccgc agccacggac ggatgcgggc tgcgatgtcg
    40861 cggcccgaac tggccggaga aagctcgtcg tagccgagat agaccgattg ccccatcagc
    40921 acgcccgaca gtgccatgat ggctatcgcg gacagcgtgt ggttgcggcg caccaatacg
    40981 ctggcggctg cggcccctgc cagccagaac agggcgccgg cctgtagcca tggtacgaag
    41041 cgggcgtaga gttcgagcgg aacttcgcta ctcgcactgc gttcgaccag cggcgccaga
    41101 ccgaacagcg ccagtgcgag cggcatgagc agcgtgaact gccatgccag gctgcgcccc
    41161 ggtgcgctgg caatgtagcg tgcggtcagc agcgcgagtg caggaaagat cggcaggatg
    41221 tagggcacca gcttggaatg cgagacgctg aagaacagga aaatgaatcc gctccagacc
    41281 cagagcatgc tttcgggcga aaaactgccg cgcggccgtt gcgacttgcc ggagagcgct
    41341 tcgcgcagcg acgcaatcag tggtgcgagc cacggcagca tgccgagggc caccaccgga
    41401 atgaatgtgt accaggggtg gacgcggccg gcttccggag taaggaagcg ctcaaaatgt
    41461 tcgtggatga aaaaaaacca cagaaattcc gggttggcac gggacacgag cacgaaccag
    41521 ggcagggtga tgagcgcaaa aaccgccagt ccggacaaca ggtacagtcg acgccagatg
    41581 tgccagtcgc gttgcaccag cgaataggtg atgagcacgg cgccgggcag ggcgataccg
    41641 atcagcccct tgctgagcat cgacaggccc atcgccgccc agcaggccag catccagttg
    41701 cgttgtgccg tggcgctgct gctggtttgc gccagcaaca gcgttgccag actggccagc
    41761 atgaagaatg tcacccccat gtcgaggatg gcgatgtgac cgatggcaaa gtagagaaag
    41821 ctgctggcca ggattgcggc agcgatgaat ccggtacggc ggtcatagag acgtgcgccg
    41881 gtgtagccag tcagcagaat gccgagaaat cccgtgagcg cggaccacag ccgcgccgtc
    41941 cattcgtgca ggccgaaccc gcggtaggct attgcggtgg cccagtattg cagcgcaggt
    42001 ttctcgaaat atttcaggcc gtccagccgc ggagtgatcc agtcgccgct ggcaagcatt
    42061 tcgcgcggga tttcggcgta acggccctcg tccggccgca ccagggcgtt gtggcccagc
    42121 gtggcaaacc acaccaccgc cagcagggca atcgccaggg ccaatgccag ggcagatggg
    42181 gcttgacggt ccggattcaa gacgcgggta acctgtgcaa ggaattcgaa acgtccatta
    42241 tagcaaaatg ggcgtttccg ggcgtgcttc gtttgatctg tggcacaccg cagacgctgt
    42301 gcgggaatgg tccgggaccc gggtgcggcc ccggtccgtg gcgcagatcg ggagtcaggg
    42361 cagatgcggc ggcatattca ggacatatgg accggaatga ttttcattaa tcgcattgac
    42421 aagccggatg ccgttggtta gattttaccg gttcgagcgt tttaattcct gcaacgcatt
    42481 ttttgatctc aaggagcatc ccatgaccgt tctcgtctgc aaacctgcgc cggatttcac
    42541 cgcgaccgca gtgctgggca acaatgaaat caaggacatc accttttcgg aattcaccaa
    42601 aggcaagtat gtggtgatgt tcttctatcc gctcgacttt actttcgtct gcccgtccga
    42661 actgattgcg ttcgaccatc gtctggccga attcagcaag cgcggcgtgg aagtgatcgg
    42721 cgtttcgatc gattcccagt tcactcacct cgcctggaag aacaccccgg tcaacaaggg
    42781 aggtatcggt caggtcaaat ataccctggt ggctgacgtc aaacacgaga tctgcagggc
    42841 ctatgatgtg gaactcgatg gtggcgttgc gttgcgcggt tccttcctca tcgaccgttc
    42901 cggcatcgtg cgtcatcagg tggtcaatga tctgccgctg gggcgtaatg tcgatgaaat
    42961 gttgcgcatg atcgacgcgc tgcagttcac cgaagagcac ggtgaagtgt gtccggccgg
    43021 ttggcacaag ggtgaggccg gcatgaatgc ctcgccagaa ggcgtggccg attaccttgc
    43081 caagcacgcc gaagcgctct gatctgctga cgcatgctgt ctgcgtcgcg ctgctgcggc
    43141 gatgacggca tgcgatcagt gccaggattg ctgtaatgca ggaaacaatg aaaaacgcgc
    43201 gtctgcgcgt ttttcatttt gtggtgttca tccgtgccag ggtgctctgg
//
LOCUS       DAIANL010000023        21453 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_834115, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000023 DAIANL010000000
VERSION     DAIANL010000023.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 21453)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21453)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21453
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834115"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4313 a         5823 c         6907 g         4410 t
ORIGIN      
        1 ccatgccaaa cagccgcgta ccgagcaccc aggagaggat gaaggtgccg agcacgatca
       61 ctacgtcaat ggttacaccg gtgacaccga catgggcgat atcctgcagg gtcagtttga
      121 gaccgaacag cacgatgcca gcacgaagca gggtctgttt ggaaaacccg acaccacggc
      181 cgcactgggg agcgatgctg tgatagaagg tgttgccgac gatcatgccg aggacaatgg
      241 caattgtcag cgcgctgaaa ccatgcgcgc cgaaccaggc aatcttgccc agttcgaagc
      301 cggcaacggc gaggatgccg ctcagaagca ggccatagaa caatggcgga tgtggtttat
      361 ggtgatgact atgtgtcatg aatggtcctt gggattaggg tttttataca aaacgtcttg
      421 taacattcag ccggttgtca ttataagctg caattatcaa ttcacaaaat gggtaatgtc
      481 gttataattg accagtgttt cgattattta acccatgaca tagtctgcag atcataccgg
      541 agtcacatga gccaactacg tttaagcctg cgccagctac agattttcat tgccgtggtc
      601 gagcatggca gcaccagcgc tgcggctgag gccatcgccc tgtcacagtc agccaccagt
      661 gccgcgctga acgaactcga acgcactctt gggatgagcc tgttcgatcg ggtgtccaag
      721 cgtttgctca tcaaccacaa cggccgcacg ctgttgccgc aggcgttgtc tctgctcgac
      781 gctgcaagcg gtatcgagcg ctgggccgag gacgagcaat tacagaccga tgggctgcgc
      841 atcggagcga gcaccaccat cggcaattac ctgttgcccg atatcctggc gaaatttcgc
      901 gccacgttgc cagaacgcat ccagccgggc tggcaggcgc aggtcatcat cgaaaataca
      961 gccgccatca gccagaaact ggtcaatttc gagttggata tcggtctgat cgagggctat
     1021 tgccacgaat cggagctgat ggtgcgcccc tggctcgaag acgaactggt cgtggtggcc
     1081 gcacctcatg atccggtgat gccgaaagcg gatgaaagct gcgtttcgat cgatactctg
     1141 cggcatgccg tgtggctgct gcgcgagcct ggttcgggca cgcgtgaaaa tatcaacgag
     1201 gcgctgttac cttacctgca tcatctgcag cgcggcgtgg aattcggtga ttcggaagcg
     1261 atcaagcgcg ccaccgccaa ggggttgggc atcagttgcc tatcacgctt cgtggtgtcg
     1321 gacatgcttg aggatggccg gctatcgctg gtgccgacgc cgctgcccaa gttcacgcgt
     1381 cgtttttata tcgtgctgca caaacagaag aagctcacgc gcgggctgtc gcgcatgctc
     1441 gattatttcc agcagatcga taacgccggc tgagcaccgg cggcgcgaac gattcagcag
     1501 gtgaaaaccc gggattccgc acctgcgggg aggctcaggt ggttttgacc caggtgtcct
     1561 tgagaccgac cgtacggttg aacaccggct gttgcgggtt gtgatcgcga cggtcggcga
     1621 cgaaataacc atgccgttcg aactgatagc ggtcttccgg cagggcctga gccagcgccg
     1681 gttcgagtcg ggcctgcacg acgcgcagtg attccggatt gatgtcgagc atgaaatcct
     1741 gtgacgtgcc cggatggggg acgcgaaaca gccggtcgta caggcgtacc tcggcctcaa
     1801 tggcctcgtt gatggacagc cagtggatgt tgcccttgac cttgacgctg tctgcgcccg
     1861 gcgtgccgga tttcgtgtca ggcagcaggt tggcgtgcac gcagagcaga ttgccctggg
     1921 cgtcgtagtc tgcgccagtg cattcgatga cgtaggcgta acgcaggcgt acacggttgc
     1981 caggatagag gcggaaaaat cccttggccg gcgtctcctg gtagtcctcg cgttcgatcc
     2041 acaattcacg cgtcagtccg accggacgtt tgccggcttc gctgtgcagc gggtggttgg
     2101 gtgcgagaca ttcctcacgc tgttcctgcg gatagttgtc gaggaccaga cgtatcggat
     2161 cgagtacggc gatacggcgc tgggcagcaa ggttgagatc ttcgcgcatg cattcctcga
     2221 gcacggccat gtcgatccac gtgtcggatt ttgatacgcc gatgcggtcg gtaaacagac
     2281 ggaaccccgc tgcagtgaaa ccgcgccggc gcgcaccggc cagcgttggc aagcggggat
     2341 catcccatcc gctgacggtg ccggattcca caagctggat cagcttgcgc ttggacagca
     2401 ccacgtaggt caggttgagg cgggcgaatt cgatctgctg tggatgccag tggatgagcg
     2461 gctgcaactg atcgagtacc caatcgtaca acggtcggtg atcttcgaat tcgagcgtgc
     2521 agatcgaatg ggtgacgcgc tcgagcgcat ccgaaatgca gtgggtgtaa tcgtacagcg
     2581 gataaatgca ccaggtgttg ccggtgcggt gatgttctgc atgccggatg cgataaatca
     2641 ccggatcgcg caggttcaga ttgggtgagg ccatgtcgat gcgggcgcgc agcacgtggg
     2701 tgccgtcagg gaattctccc gccttcatgc ggcggaacaa ttcgagattc tcgtcagggg
     2761 tgcgattgcg gtaggggctg tcctgaccgg gttcggtcag gttgccgcgc cgggcacgga
     2821 tctggtcggc gttctggctg tcgacatagg ccaggccgtg ttcgatcagg tattcggcga
     2881 acaggtagag ccggtcgaag taatccgaag cgtaatactg gtgttcgccc cagtcgaagc
     2941 cgagccagcg caccgcttcg aggatcgaat cgacgtattc ctgttcttcc ttttcggggt
     3001 tggtgtcatc gaagcgcatg tggcatgcgc cgccatagtc gcgtgccaga ccgaaattga
     3061 ggcagatcga tttggcgtga ccgtagtgca ggtagccgtt cggctcgggc gggaaacgcg
     3121 ttgccacgcg cccaccccat ttctggctgg cattgtcgcc gtcgatgatg ttgcggatga
     3181 aattgtgtac tgcgggtttt tcggcggatt cggacatggt gtgccagttg ggcggttgat
     3241 cgtataatcg ggtacttcgg aacaatgaca gggatggtaa tgcaacggcg cgattttatc
     3301 aaagggacgg ccgggctggg tctggccgga cttcccgtag cggcggccca ggcagcagat
     3361 tatccggaaa tcagctggcg catggcttcc agttttccga aaagcctgga caccatctat
     3421 atggcggccg agcagatggc ggagcgggtg gcggcattga ccggcggcag gttccatatc
     3481 cgcgtgttcg ctggtggcga actggtgccc gggctgatgg tgctcgatgc agttcaggat
     3541 ggcacggtgg aatgtggcca cagcgcgagc tattactatg tcggcaagaa catggcgttc
     3601 gcctttgact gcgccgtgcc gttcggcatg aatgcgcgcc agcagaacgc ctggatgtac
     3661 tccgggggcg gcatcgaact gatgcgcgaa ctgttccgca gctaccatat cattcagttc
     3721 cccggcggca acacgggcgc gcagatgggt ggctggttcc gtcacgaggt gcattcgctt
     3781 gccgatctca aagggctgaa aatgcgcatt cccgggctgg gcggcaagat catggcgcgg
     3841 ctgggcgtcg tgccgcagac gctgccgggc agcgacatct atccggcgct tgaaaccggt
     3901 gccatcgatg ctgcggagtg ggtcggcccc tacgacgatg aacggctcgg ttttcacaag
     3961 atcgccaaac attattatta tccaggctgg tgggagggcg gcccacagct gtcgttcatg
     4021 gtcaacctgt cgaaatggaa cgccttgccg aaaatctacc agcaggcatt cgaagtggcg
     4081 gcagctgaag tcaatgtgcg catgctggcc gaatacgatg cccgcaatcc accggcgctg
     4141 atgcgtctga ttcagcaggg tgtccaactg catgcttttc ccaaggatgt catgctgact
     4201 gcacggcgtg aatcctatgc gctgtttgag gaagaatcgg cgcgcaatcc gatattcaag
     4261 cgcatctatg accagtggaa agtgttcctg cagagtgagc ttcagtggtt caaggtgtgc
     4321 gaatcgcctt acgacaattt catggattac gtaaagtagt ggctgcagca acctgtttat
     4381 gaattttttg cagtcactcg gcgatgagcg cagccggcgg tgcgagacgg cccagggcaa
     4441 ggcgggcgct gccggtggcg gcctcgctgt gctctaccgt ggtcacggga acgcctagca
     4501 ggcgttcacg cagcgccatc cattgtggat tgcggctgcc gccgcccgcg gtgaacacgc
     4561 gtcgtggcgg cggagcgcca agtgattcga gcagctggta gccttcgcgt tcgatgtgcg
     4621 ccatgccttg cagcagaccg tgcagaaagg caaccggttc ggtgggacgc ggcgtgagcc
     4681 gcggttcgag ctgcgggtca tgacgcggaa agcgttcgcc tcgcgcgggt agcggataat
     4741 agtcgagcgg actgtcgtgc agcggatcga tccggcaact gtaatcgcgc agctgcgcat
     4801 catcgaaaaa ctggcgcagt accgcgccgc cgctgttcga tgcgccgccg gcaagccaga
     4861 attcgcccag ccggtggctg tagacaccat ggatcgggga gacaatgggt cgaagggaga
     4921 ccaccttgac cgtgagcgtg ctgcccaggg aagtgagcgc gtcgccgggg cgaagcaacc
     4981 cggccgcaag cgcggcagcc gtgctgtcgg tggtgccggc gcgaacctcg caggcgggat
     5041 cgagtccgaa acgttgtgcc agggcggcat ccagccggcc gagcaggctg cccggcgcca
     5101 ccacatcggg cagcaatttc cgtgcggggt gttgttgcag cacttcttgc cattccagcc
     5161 gcaccgggtc gtaaccggtt ttgagtgcat tgtgccagtc gctcggtgca ggccggccga
     5221 gcaagcggct gttgagccag tcggcctggt gcagcaggtg tcgcgcctgt tgtgccgtat
     5281 cgtgtgcgag cagccagtcg agcctggcgc ccggctgcgc gggcaatggc tgaaaatacg
     5341 gcagcacggg cgtgatcggg tgcaatgccg agtcggtcag gacggcggtg ccggaggtgg
     5401 cgtcgagcgc aatggcgtgc agcgtcgctc ccagttctgg cggaatggcc gccatcagtg
     5461 cgtcgagcgc ctgctgccag cattgcggat ccatggcctg ccggctgttc cattcgatgc
     5521 gccattgcca gcgcggttcg ccgtcggcgc cgcaaatgca ggcgcgtgcg ccggaggtgc
     5581 cgaaatccag tcccagagcg tgcatgtaaa gaattcgggt acaatcgaag tttgccagtg
     5641 taccctgatt ccgctcgcgt gcgcctgatt gccatcaaac ttgccgggtt caaatcgttc
     5701 gtcgacccgg tgcatattcc ggttgccagc cagatggtcg gcgtggtcgg acccaacggt
     5761 tgcggcaagt ccaatgtgat cgatgccgta cgttgggtgc tgggcgagtc gcaggccagg
     5821 catctgcgcg gtgccagcat ggcggatgtg atcttcaacg gatccggtgc gcgcaaaccg
     5881 gtgtcgcgtg cttcggtgga gctgttgttc gataacaccg atggcagcgc gccgggagcg
     5941 tggtcgcaat atgccgaact gtcggtcagg cgcgtgctga cccgcaacgg cgattccaca
     6001 tactacatca accagacgca ggtgcgccgg cgtgacctgc tggacgtatt cctcggtacc
     6061 ggactcaatg cgcgcgcccc ttatgcgatt atcgaacagg ggatgattgc gcgcatcatc
     6121 gaagcgcgtc cggaagaact gcgcggcttc atcgaggagg cagccggcat ttcgcgctac
     6181 aaggaacgcc ggcgcgagac ggagagccgg ctcgaggcca cgcgtgaaaa cctgctgcgt
     6241 gtggaagatg tgcgccgcgg gctggcggag cagatggagc gtctgacggc tcaggccgaa
     6301 accgccgggc aatggcgtgc gttgcaggca gagctgctgt tggcgcggca gacgttgtgg
     6361 ttgctcaagt tgcgcgatgc aacggaaaaa agccgtcagg ccggtgcgcg cgcgcaggca
     6421 ctggagcagg agaaacaggc gggcgagcag gcattggcgc tgttgcatgc cggactggcc
     6481 agtctgcgca atcagcagga cgtggcgagc gccacactca accagaaaca gggtgcactg
     6541 tatgcggtga tggcggaagt gtcacgcctg gaacaggtgg tgcgccatgt ggccgagacc
     6601 cggcagcgca ttcggcagca gatcgggcag ggggaagcgg cccaggccca gcgcgatgcc
     6661 cttggcgaat cggttgcgcg cgagatcgtg caatggcaag agcgtgcagc acatacccag
     6721 gagcagaagc tggcctgcga gcaggaacgc gaccgacagc gcgcacaatg gccggcgcat
     6781 gaaacggccg aacgcgaggc gcgcggccag gtggccgccg cgcagcacga gctggcgcag
     6841 aacagtcagg cggagcaggt gacgcgtacc catctgcagc atgaaacacg ggcacaggcc
     6901 gagcgcgagc agcgactgca acggttgcat caggaagagc aagggctggc gccggtcgat
     6961 gctgctgtgt tgcagcagat cgaaacacgt caggccgaac tggcctgcca gttcgaagct
     7021 gccgagcgca atgtgcggca aatgcgcgat cagacgggtg aactggagca gcagatcaag
     7081 ccgctgcggg agtcgctcga acgcttgcgg cgcgagttgc accgtaccga aggcgaactg
     7141 gccggattga ctgcggccca gcgcgatgcg cgcgccagtg aggcgatcga tggctggctg
     7201 gcccggcatg cgctggctga tgtgcaacgg ctgtggcaat gtctgcgtgt cgagcctggt
     7261 tgggaactgg cggtgcaggt ggcgctgggt tcgcgcttgc aggcgctggc gctggatgca
     7321 cctgccgacg cgcagctgct tgccgatgcc ccgggcggag ctgtctggtt cgatgccagt
     7381 gcgggtgcac ggcttgcgcc ggccgaagcg ggtacgctgg cgcagtatgt tcaggtccag
     7441 gctgcagggg atgccgccgt acgcgactgg ctggccggct ggtgggtggc gtccagcgtt
     7501 gacgaggcat tggcgttgcg tgaccagttg ccgtccggag tgcgctgcgt cactcgcgat
     7561 ggtcatgtgc tggcacgcta cgcagcgcag cgggcaggct cgtcggatgc gggacagggt
     7621 gttctcgaac gccagcgcgt catcgatgcc ctgcgggcaa ccatggaggt gcgcgaaggc
     7681 gaacagggcg aacaggagca acagatcgcc cggcttgacg ctacgcttgc cggattgcgc
     7741 cagcagctgg aacaggaacg cagccgggcg ggccgcctgc aacaggaacg tcaccaggcg
     7801 cagctggagg tcgagcgcac gcgccagcag catgaacgtc tcgctgcccg gcgcgaacag
     7861 atcgcagcga cacgcgaaga actggcaacg gcgcttcagg ccggcacagc cgagcgcgag
     7921 cggctcgaag cgcgacttgc gcagcaacag gcggccggcg agcaatgccg cgcaagactc
     7981 gagcaggcgc ggactgaacg ccagcgactg gaaacggtgc tcgccgcaca gcgacaggca
     8041 ttgcagcaga gcgaaaaaca ggcgcaggag tgggattttt ccgcgcgtct ggagcgggaa
     8101 aaactggccg agttgcagcg aaagcaggcc gatctgcttg aacagtctga aaaattcata
     8161 cagcataaca atgtgctgag cgatgaactg aaggcgctgg atgaaaatac gccggcggca
     8221 gaactcgatg ccgcactggg agaacgggcc gtggccgagc agacgctggc cgaggcgagg
     8281 aatgcactgg aggcactacg tgagcagttg cggttgctgg aggaggagcg catgcaccag
     8341 gaacagcagc tgtcaccgtt gcaggcccag ctcgaaaccg cgcgcctgcg ccagcaagag
     8401 aacacgttgc tcgaagcgca atgccgtgaa gaactgcagc agctcgatgc cgatgagcag
     8461 gcgctggccc cgcgtcttgc gcaggcgcgt gcgcccgcac ttcaaggcga gatcgagcag
     8521 ttgcgtcaac gcatcgatgg gttgggtgcc gtcaatctgc tggcgcttga cgaactggaa
     8581 caggcgcgac agcgcgatac ctatctgcag gctcagtgcg atgacctgct gaccgcagtg
     8641 gctactctgg aacaggccat ggcagtgatg gatcaggaaa cgcgtgaact gttccgcgcc
     8701 acttttgaca cggtcaatgc ttccatggcg gaactgtttt ccacactgtt cggcggcggg
     8761 caggcacatc tgggcatgac cgagggtggc tggcttgaag ccggggtgca ggtgatggcg
     8821 caaccgccgg gcaagaagaa cagttcgatc cacctgctgt cagggggcga gaaaaccctg
     8881 actgcactgt cgctggtttt tgcgctgttc cgcctcaatc cggcgccatt ctgtttgctg
     8941 gacgaagtcg atgcgccgct cgacgacacc aataccgaac ggtatgcgcg actggtgcgg
     9001 caaatgtccg acagcgtgca gttcctcttc atcacccaca accgcatcac catggaagcg
     9061 gccacgcagc tgatcggcgt taccatgcag gaaccgggtg tgtcgcgcat cgtctcggtc
     9121 gacctggaac aggccgagcg tatggtacag cagtccggag cggtgacggc ttgatatgtc
     9181 ccgatccggc aatgcgtcca tcacgtattg caccgctgga aaaggtaccc gctttctgtt
     9241 gcgtcacacg caatctccct ggcttgattc cgggcctgag tgcctgaata tccggcatct
     9301 gccacgttcg gtcgggtcac agtctgcgtg gattttcccg ggtaactaaa attaccaaca
     9361 ggtcttaatt tcatgtttac tattaatcta atattcgtta gaatgtttta attaggtagc
     9421 cgatatgact gacagtatca cgcgcccaat gatgcaaggc atatgcgtcg atggtgggcg
     9481 accagaaaat aacctgcaat ggagacaagc catgaaaacg ttgctcattt tcaaccatgc
     9541 accatatgat tcaaccgatg tgagctggaa cggcttgcgc ctggccgata aactgctcga
     9601 tgcagggtgt gatgtgcgga tattcctgat gaatgaggct gtcgacatgg cccgggatgc
     9661 ctgcgtgccg ccgcaaggct acgatcagga tctgacccgc atgctcaagg cgctgatcgt
     9721 ccgtcatgcc acggtcaagg tgtgcggcac ctgcatggcg cgttgcggga tttacaagaa
     9781 ccagccgtat tttgatggtg ctgaaacatc gacgatgcag gcactgtcgg attgggtcgt
     9841 tgacagtgac aggatccttt cattctgatg agcgggtgta tcggtgcagc cacttttccg
     9901 cctcgaccgc agctgcacaa gtcatcgcga cagcgatgat ccatgaccat tcccgtgcgc
     9961 cgatgggcgc cgtttgaaat acctcgttga agacgggcac ataggtgaat accatttgca
    10021 gagcgagcat cgcacatacg ccgacccaca cccatgggtt tgaaaacgcg ctgatcggcg
    10081 ctcctagagt agccagaaca ccagcgctga ggtcagtgtg gctggcagtg cggccatcag
    10141 caatccgacc ggactgactg aattttcttt gaaatgccgg cgcaagatgg ccagacctgc
    10201 gggattgggt gcgttggcaa tcacggtcag cccgccgccg gtaacggccc cggccaccag
    10261 agcgtatttg aaaccgtctg acagtccctc taccagcgag ccgagatagg tcagcgctgc
    10321 gttgtcggta aatgctgtca ggaaggcagc gccgaggaac acctggtcgc tgcgcatacc
    10381 catcagcacg ggttgcagcc accattgctg ctgtccgccc aatattacca acccggccag
    10441 aaaaaaagcg accagcagtc cttcgcgcag aatcagccgt tcctgatggc tccggtacgc
    10501 ttgcgccacc ccaaggaaga acaggaacag ccccatgaac atggccgggt ggtgggcaaa
    10561 catcacgata ccagcgagaa tcgccagatg taccagcgtc aggatgaacg ggacgggctt
    10621 gccactgtct gctgcagtcg ttggcaggtg tgccagttct ttgcggaaca gtagcgtcgc
    10681 acccaaggca ttaatgacaa ccgcgatgac tgctttccag ccgaaggtcg tcatcatgaa
    10741 gttgaagccc cagtgccact tcatagccac catcaacacc ggcggcgcgg caaacggtgt
    10801 caggataccg ccgatggaca cattgacgaa cagtacgccc agcgtcgcgt atttcaggcg
    10861 cacggaaatg cccttgccga aaaagtggtc ggcaaggatc agggctgcca gcgtcatcgc
    10921 cgcaggttcg gtgatgaaag atcccagcag gggagtcagc atcagtacgg tgaaatagaa
    10981 gcccatgccg cctggcaggg ggatgaggcg cgccaccaat cgtacgccag ccattaccgt
    11041 gtgcaggatc gggcgagtgc cggcgaccac catgatggca aacacgaaca tcggttcggt
    11101 gaaattgcgc gagtcaatgt atcgcgtagc cacgtcgacg ccaccggtgg taaacatgac
    11161 cgcggctagc accatggccc agaagccaaa caccacttcc acttcgccca gcaaatgcca
    11221 aacgccggca tgtgttggcc gggtgtgcgc cagatgctcg aaaaagcggg tggtgaaagt
    11281 gtgtacgatg gcgaccgcaa acagcacggc gctgatcact tcgatgccgg tcggtgtcac
    11341 agcttttccc gtgttatgac agattggctc aaatcccgtc gttttgccac atgcagcaat
    11401 gggatcatgt caggacagca accaatataa atcagattgc tggcgccaga gtaataaatt
    11461 cgcccatgaa gcaaggggat gctgacagta gttacacgat cagtgtgccg attgcgttga
    11521 atcagcggtt tcggcatgcg gcgggaatgg cactcgaagc agtgatcgca gcatgtagat
    11581 cgtgatcaac ccgggagatg gtacgatcaa actccgtttc gatagaagaa aagtcgccag
    11641 ccttgaaatg gtcagccaat cgtagtcccg gctacgactt gcgccattcg gttcgcgatc
    11701 atcacgattt cacctacaac gcgattcgtg gagaattcat cgccatgtca ttaagcagtc
    11761 gttcatgaag ggacggaatc ccagagaatt gtaaatgaac cgcttgcgct gtaatgttgc
    11821 gatgtgcgga aatggaaaaa cgccggagat caacccctta cagctgcgac atttgcaacg
    11881 tcgtccgtgc agccatggat gatttcatcc tgtttctcgg ccttttccat cagggtcatg
    11941 ttggcttccc tgcgctttat gcagtcctcg atctggtgcg tataggcatc ggactgggct
    12001 ttgtccagcc cacgctcaac atccggatcc tgattggggt tgagtttcga cggcatcctg
    12061 cattgaccat cgagattctt gtaaccagtg tcgcaggcac aatgagtcgt cttgacattg
    12121 ccggtcactt caacccgtga aacatgggca tggtcagggc atgcgtcctg acccgatgcg
    12181 ctctgggata cagccagaaa aagcatggcg gagagggcgc tgtaagccag gttgcgcaat
    12241 aggcatgcag ggtggatgaa agaggaatgg ttcatcatgg atccagtact gttttgttga
    12301 ccagttgctg cgcctggggc tgcaaagcgg aaatctggtt cgttacattg ctctgctgat
    12361 tcgtcgcaga gatcaattcc tgctgatggg ccgagggatc aggcgtctgc ttgatggcct
    12421 cgatctggtt ttccagggca tcacgcctgg caagcaaggc atcggctttt ttaatgaggg
    12481 acttgccttg ttggctcccc tggagctttg accagtccga tgaggacatg ccggggaaaa
    12541 cggcagcacg aatggtttct acaggatgtg ccggaggtgt tgccagattc gtggctggca
    12601 tttttactgt ggcatcggtt ggggtcactc cgccgtcgaa caggcgtcct gtggactctg
    12661 agccgccgtt ggtcagctgg cccaaggcag aactggcagg aagcgccccc cggtccggag
    12721 ccgtcgggag ggcctgggct gcagcagttc tggctcccgg attggtctga acaacgactt
    12781 ggcctcccat ttgtgggtcg tcatccaaag atttcatttc tccctgaaga tctgagaccg
    12841 cctgctggtg ttgctgggcc tcgttctgat tcatggcagc gcgctcagcc gcgacgcgat
    12901 tcgcaatctg ttgctgttca tttgcaatct cttgtctgcg ctgtgctgca gcgactccag
    12961 cctgaaagac gccgagcaca gcgttgccca aggctctgcc gattgcggca cctgcctggg
    13021 cggcaggcac cgacccggcg gatgcggcgg ttgattttgg atagcatcgc ggcggattgg
    13081 ggacaggcgt tccacccgtc gatgcgcacc attgggcaaa ttcttcccag ttattgaatc
    13141 catcagccca tgcactgccc gacaagatgc ctagcaagac tagcgagagc ataaaaaatc
    13201 cggttcttgc catcgaattc cccctgtgct acaggcgcgt gtcttgtggt gtacgcctca
    13261 gtaccccatt tttactgtcc attcagcaat cttgccctgc gcgaaagcgg catccggagc
    13321 atcaggcacc atgtcaaggt aactattcat gtcggtgatt gcaagatcgt agctgcccgt
    13381 ggcgccaagc agcatggcag tgttgaacac accttttggc cagcaaggat aagttctgaa
    13441 aacttgcgaa tactggtatc cggccgcatc cagatcaccg tgctgataag cctgctgtgc
    13501 aagcagcgca tgcgagcgtt cttcgtcgtt catcggcggc atggtcttcc agttggcgca
    13561 ctggatccgg tgcgcctgcc tctgggaatc aaggtacaac tggaagtaac tccgcatgtc
    13621 cagtgccagg actttcaggg cccaggcgat ctgttgcgca tcggattgcg accagcccga
    13681 ccagagccaa tagtagtgat ccatgtggac gttgtcagtc caggcgatct tccatgcagg
    13741 aagcattact gcgcttcctg gtacatagga agtcgtgatg gtgctgggaa tgaccgagtc
    13801 cagatatgtg gtgtatttct ggccgtcctg ggggtttgtc agatcgatcg agtcggtggg
    13861 accatgatca ttctggaaaa cgggggtgct actcgaatag cgctgcaatt cttcttgcag
    13921 gatgcttcgt gctttcagca cgtccggcga aggagagtag gttggggctg ccaagccttg
    13981 ggaagcaaaa aaaatggtta cgacaaccgt gctcaaaagt gtcaatacgc gtgtgagccg
    14041 cattttttca cctgatcggt taatggcgcc ccagaggcgc cagagtgttg tgcgcaatct
    14101 ttgaagcagc cattctttaa caaatgtatt atttgttgtc aagactgacg atgaaggaat
    14161 gctacacaat ccctgttttt tttgcaatct gtgtgttgag gtctgtagag aacaaatcag
    14221 ctgtccgggc ttgcgcctga ctcaggatga agcagcgcgg ctgcttgtgg tatgcgagcg
    14281 atttccattg ggagtccttg agcatgcgcc gatccattgg ttacggcaat tctcatgcca
    14341 ttactgtgaa cacgctctgg cgccagagtc ataaattcgc ccatgaagca agaggatgct
    14401 gatagtagtt acacgatcag tgtgccgatt gcgttgaatc agcggtttcg gcatgcggcg
    14461 ggaatggcga ttgcagggtg ttcgggcgtg gattcctcca gcgatgccga gtgggaatgg
    14521 cattgccttt cgcgccctgg gtgatgccgt gagttcattg tgaagcatgc aacggatgag
    14581 accggccctg cttctgcaag accggtcagg tgaaggatta ctgcttgctg tgatcggtgg
    14641 ccatcagcat gtacattccc ggtaccacga acagggtgaa cagggtaccg atcgacagcc
    14701 cgctggcaat gaccagtccg agattgaagc ggctgaccgc accggcgccg tgtgcgatga
    14761 tgagcggcac cacgccgagc accatggctg cggtggtcat caggatcggc cgcagacgta
    14821 atccggctgc cttttcgatg gcatcacgtt tggacagccc tgcacgctgc tgattgttgg
    14881 cgaactcgac gatcaggatg ccatgcttgg aaatcagacc aatcagggtt accagaccga
    14941 cctcggtgta aatgttgagc gaggctccac cgatgccgag attgatgaaa atcagtgcac
    15001 cggcaatcga catcggtacc gaaaccagga tgatgaccgg gtcacggaag ctttcgaact
    15061 gcgccgccag ggcgaggaag atgatgatga tcgcgaagaa gaaggtgagc agcaagccac
    15121 ccgattcctg gatgtactgg cgcgacggtc cggagtagtc aaacccgtag ttgggtggca
    15181 gggtgcgctg cgccagatct ttcaggtagg ccagcgcctg tccctgggat acggtcgggt
    15241 agttcacccc ctggatcgtt gccatgttct gttgctggaa gtggttgatc gtctccggct
    15301 cggtcgaagt ttgcaggtgc gccacattgg acagcgccac catgtcgccg ctggcgctgc
    15361 gcaggtaata gttcttgagc tggtcggcat tgaggcggaa gcgttgctgc acctgcggga
    15421 tgaccttgta ggaacggccg gccatgtcaa agtagttgac atagttgccg cccagcaggg
    15481 ttgacagcga attgccaatg tcggccatcg acaggccgag cgcagcggcc ttgtcgcggt
    15541 cgatcaccac agtggtctgt ggcagatcaa tgcgcagatc ggactgcagg aagatgaaat
    15601 tgcccgactt catggcttcc tgaaggaatt tttgcgacac gtcatacagt tttgcgaacg
    15661 gctcggtagt ggtcagcacg aactgaaccg ggaaaccgcg cgcaccgggc aggggaggcg
    15721 gctggaatac ggcgttctgc gagccggcga tgctgttgag ctcctgttgc acgatcggtt
    15781 gcagcacatt ggcattggtg gtgcgctggt cccatggttt gagcaccatg ccgctgatga
    15841 tctgcgttgg catgtcgagc tggaaaatgt gttcggtctc gctgtggcgg ttgaacacat
    15901 cgcgaatggc tgcggtctgc gtttcgcgtt gcatcaggcc ggcgtcgggg gcgctgaagg
    15961 tcatggcgat cagcacaccc tggtcttcct gtggtgccag ttccgacatt gatgtggtat
    16021 agaggaaata gatgcccccc agcagcagga tggcgaatac cgccacgacg gacaagtggt
    16081 tgagcacgcc atcgaggcgg cgctcgtagg catgatcaag gcgggcgaac tggcgatcga
    16141 gccaaagcac gaggtggtcg atccagttgc ctccttccgg ttttggcgcg cgcagcagtt
    16201 tcgagcacat cattggcgac agcgtcagtg cgatgacggc tgacatggtg acggcgccga
    16261 ccagggtaaa ggcgaattcg gtgaacaggg caccggtcag gccgctcatg aaaccgatcg
    16321 gcacgtacac ggccaccagc acgatggtca tggcaatgat cgggttggcg agttcgcgtg
    16381 cagcctgaat ggcggcatcc atcgggctga gcccttcttc gagatgtcgg ctgacgtttt
    16441 caaccacgat gatggcatca tccaccacca ggccgatggc gagtaccagt gcgagcaggg
    16501 tgagcaggtt gatcgaataa cccagtgcca gcatgacggt aaaggtaccg atcagtgaca
    16561 gcggaatggc gatgaccggg atcatcaccg atcgcggcga accgaggaag atgaacacga
    16621 ccagagtaac gatgccgatc gcttcagcca gggtcttgat gacctcgtca atcgacgaat
    16681 tgacgaattt ggtcgagtcg tacaccacat tggccttgag gccgttgggc agttgagcct
    16741 ggatctcggg catcacggcg cgcacgcgtt tgatcacgtc gagcaggttg gcactgggcg
    16801 caacctggat gccgatgtac accgctttgc ggccatcgaa ggccacgctg ctgtcataat
    16861 catccgaacc gaggctgacg tgggcaacat cgcccagacg tacgatgttg ttgccttgct
    16921 gcttgacaat gatggcacgg aattcgtccg ccgaatgcag cgaggtcgag gcgttcagat
    16981 tgatctggat catctggcct cgcgtctgtc cgctcgacga gaggaaattg ttgttgatga
    17041 gcgccgtgtt gatgtcggct gcggtcaggc cggcggcagc cagtttttgc gggtcgagcc
    17101 aggcgcgcag ggaaaaattc ttggcgccga gcagttcggc cgtctgcaca ccttcgatgg
    17161 cctgcagttt cggctgcact gcgcgcaaca ggtaatccgt gacctgattg ggcttgagca
    17221 cgtcgctgta gaagccgatg tacatcgcgt cgatggtctg gccgatactg acactcagta
    17281 cgggcttctg tgcctggggc ggcatctggt tgagtacggc gttgaccttg gtgttgattt
    17341 cggtcagcgc cttgtcggga tcatagttca gccgcaggtt ggccgtgatg gtactcatcc
    17401 cctgcacgct gttcgaggtc atgtagtcaa tgccgttggc ctgggcgaca gcgttttcga
    17461 gcggagtggt aatgaaactc gcgaccaggt tggcatcggc gccgggatag gctgtcgcta
    17521 ccgtgacgac tgcgttttgc gtataaggga actgcaatac cggcagcagc ccggctgaac
    17581 gcagccccag taccagcatc accaggctga tgactgtcgc aagtaccggc cgatggacga
    17641 acaggtcggt aaatttgcgc atggtatcgg aaccggcggc gctcattcgt cacccacttg
    17701 cggatgtgca tcgttggtcg gctggatgct gttgtcgacc agtaccggcg cgccgttgtg
    17761 cagcttgagc ttgcccgagg tcacgatcac ttcgccagga cggataccgg tcatgattgc
    17821 gacctggtca ccgcgggtca ggccggtagt gacgaagcgc tgttcgacgg tcagttgcgg
    17881 ctgacccttg gcgttcttgc cgtgttcctg caccacgaat accgtgctgc cgtaggggtt
    17941 gtaggcaatg gctgcattgg gcagggtgat cagtcgctgt tgtcggttct ggtcgatcct
    18001 gacggtcatg aatagcccag gcagcagctt gccatccgga ttgggcaggg cggcgcgtat
    18061 cttgatgttg cgggtgttgg catccacctg tggctcgacg gcggtgatcc tgccatgcag
    18121 gatttttccg ggaagggcgt cgctgcgtgc atcgatttga agtccggtgc gtaccagggc
    18181 gaggtcgacc tgcgggatgc tgaaatcgag gtagatcggg tcaagtttct gcaaggtcac
    18241 gatggcggcc cctggtgcaa ggtattgtcc cggatcgacc tggcgaatac cgagctttcc
    18301 tgcaaacggc gcacggatca ttttctgcgc aatctgtgcc tgttgcgctg caacctgtgc
    18361 attggcgccc tggagcgtgg cggcgtcggc atccacggtg gactggctca cggcctggat
    18421 gtgcagttgg gccacatcgc ggtcgtagtt gattttggcc aatcgcgcag atgccttcag
    18481 ttgctgcaat tgggcgatca gtggcgccgc gttgagttcg accagcagtt tgccggcggg
    18541 tacattttcg cctgaggaaa aatgaatcgc ggtgaccagg ccaccggttt cggtcgacag
    18601 gctggcgcct ttgaccgcac gcaggctgcc aatggcatcg atggacggat tccatgtgtc
    18661 atagccggcc acggtggtgg ataccgtttg cggtggcatg cccttgccat gaatcatttt
    18721 cgcaatcatc gagttgcgga actgctggaa cccgtagatg ccgccgaaca gcagtgccag
    18781 ggccagcacc gtgatgaaca tctgtttgcc tgagcgttgt ttcatagcgt tacctcttgc
    18841 gtccatggcg atgtgaacgg catggcgtat tgtgcatgcg agcgaaaata gacagccaga
    18901 tttgcctcac cggaacgtga ctggaccgaa ggtttgtcct ggtctggaat ccaggcaccg
    18961 cccatggcct ggtagagcgc ggcggtgtca gtcaggcgca gggtttgcgc gccaatcagg
    19021 ttgatgcggg tctgttgtgc ctgttgctgg gcggtgagcc attgcagata gctggcgctg
    19081 ccgagccggt attgctggcc gaccagatct tgcgacgctt gtgcggcgtt gtcggcgttg
    19141 gcgtcggcgt tgagcgtttg tgcgtcgtcg tccagtgcgc gcagtgcatc cgccacgttg
    19201 cggaatgcct gaagcacggt ttgccggtaa ttggcgccga cggcttcgag gttggcccgg
    19261 gcagcaagca cgccagcgtg cagtccggcg tcaaacaacg gttgcgtcag ctgcgcggcg
    19321 agagaccata cggcggcctg cgggttgaac aggctgccgg tggtgagtga ttcctgcccc
    19381 aggctgcctg tcagattgat ttgcgggtaa aggcccgcaa ccgcgctgcc atattgcgca
    19441 ttggcgctgt gcagcatggc ttcagcggcc tggatatccg gacgttgccg caccagttgc
    19501 gagggcacca cctgcggcag gtgttccggc aggtggaagt cagcaagcgt gaaatcgacg
    19561 ggctggaatt cgccgggtgt cttgccgcac agcgtggcga tcaggtggtg ttcctgctgc
    19621 atgcgctcat tcagtggcgg cagggtggcg cgtgtctgtt ccatctgtgt ttgcaggctg
    19681 agcacatcgg cgagtccgag ggcgccgatc gactggcgct ggcgcgcaat gtcgagctgt
    19741 ctgcgctgga tatcgagaat ctgctgcgtc gcctcgatct gtgcggcgat accggcttct
    19801 gtcatggccg cagtgaccag attgccggca agtgtcagtc gcgccgcacg cagttcgtgc
    19861 gcctggtagt cgctttgagc ggcataggct tcaaggctgc ggcggttggc gccgaagaga
    19921 tcgaacagat agcttacgcc gacattggcg ttgtacaggt tgaatatgct gctttgcccc
    19981 ggctggccga atgctgccgg gttgaacagc atgcgttcgc cggacaggtt gaggttggcg
    20041 cgtgggtaga gcgtcgaacc ccgttgtgcc gccagcgtct gctgagcctg ctgcagcgtg
    20101 gcctgggcgg cggcaatggt cggattggcc gcaaacgcct catcgatcag ccggttgagc
    20161 ttgggcgagc cgaagctgtg ccaccactgt tcgttgccct gggtcgacac ggtttcgaaa
    20221 tgctgtgccg aacccagcgc cacgggggtc gattgagtgg tggctggcag cgggctggca
    20281 gtatagcccg ttaccgcggg cgcttccggg gcatggaaat ccggacccat cgtgcagcca
    20341 gacacggcca gcaatgcgac gagaggggtg attcgcgcag ggcggaaatt acgcatggtc
    20401 tgttgcctcc ggtcagtggc cgtcgcccgg atcctgcggg gcttcagaaa ggggcggcag
    20461 atgtgcctgc agggcggaca gattgcccag cagggtgtgg aatacctgtt cgatgtggtc
    20521 cttgtcttct gcgttgaatc cctgccatat ggcttcgtac aaggtccatt ggcgcggcat
    20581 gactttctcc accagcgcga tgccggccgg tgtcagcgtg aggatgatct tgcgccggtc
    20641 agattcgcat ccctggcggc tgatccagcc tttttccacc atttcgtccg ccagtcgcgt
    20701 gatgttggtg cgcgaggaga cgatgatgtg gctcaggtcg ctcggataga tgaagcgttc
    20761 cgggctggaa tacagcatca tcagcgcggt ccacactgta tcgttgaggc cataagggtg
    20821 caggttatga ttgaccagtt cgttgaattt cttgtaaatc atgaactgca tgcgcgacag
    20881 cagcacttcc ttgcgcggat ggccaggaaa gcgctggccc atgatatcca ggcgctgttg
    20941 aaactggaga atgcaatcat tgcccatgat gcgctgcgaa ccgtgcagtt aataacggaa
    21001 gtaattatat tacccggtat tattcatcgt caattgtttt tggaagccgc aaaaatgcgt
    21061 acggcaaacg atgccaggcg gtccggcttc aggcgatgtg cttcgaacgc aggcagcgtt
    21121 gttgcccatg cgtcgtcatg atgcccgcag ccgtcggaaa cgtacggaca gactgttata
    21181 accgctgttc gtaccgcaaa tgcctgatcg ggcaatagcc gcttgtgagg tttcatgaca
    21241 ccattgcagc gtgacactgg catttcgcac aggttaggcg catacgcagt tgtggcaacg
    21301 gactgtattt tcagatgagc ataagcacac aggagcgaaa tccggcggga gatggctcgg
    21361 cgttgcgggg ccatcacggc agcgtacgca tgcgtcacat ccatttcact tcgtataatg
    21421 aatattatgt taaataatat ggtaaaacga aat
//
LOCUS       DAIANL010000024        10916 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1448522, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000024 DAIANL010000000
VERSION     DAIANL010000024.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10916)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10916
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448522"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2050 a         3182 c         3561 g         2123 t
ORIGIN      
        1 tctggaaccc gttgccattg gccgcgaagt actcaagtcg ctgctgagcg atctcggaaa
       61 gcggaccgtg cgcgatcgca acgactgtcg actgtttctc tcctcggccg acccttcccc
      121 gtaactggtg cagttgcgcc agcccgaagc ggtcggcacg gtggatgatg atggtgttgg
      181 cagtggggat gtcgataccg gtttcgatga tggtggtgca cagcaacagg ttgatctgct
      241 gctggtgaaa caggcgcatg acctgctcaa gttcgcgttc atgcatctgg ccgtgcgcca
      301 cgcgaatgcg cgcttccggc agcagttcgt tcagcgtgct ttccatgtgg tggatggtgt
      361 ccacctcgtt gtgcaggaaa tatacctgac ccccacgctt gagctcgcgc agcacggcct
      421 cgcggatgat gccaggcgaa tagcgcgcaa cgaacgtctt gatcgacagg cgcttctgtg
      481 gtggcgtgct gatgaccgaa aaatcgcgga tgccttcgag cgacatggcc agcgtgcgcg
      541 gaatcggcgt ggcggtgagc gtgagcatat cgacctcggc gcgcaggcgc ttcatctgct
      601 ccttctgtcg cacgccgaaa cggtgctctt catcgatgat gaccaggcca agccgtttga
      661 aatgtacgtc attctgcagc agtcggtgcg tgccgatgac gatgtcgacc gtaccgtctg
      721 ccagcccctg caaggcctgc gcctgttcgc gtgcactgcg gaagcgcgac agttcggcga
      781 tacggatcgg ccagggcgcg aaacggtcgg aaaaggtttg cgtgtgctgt tcggccagca
      841 gcgtggtcgg caccagcacc accacctggc gattgtccat cagtgccacg aatgccgctc
      901 gcagcgcgac ttcggtcttg ccaaacccga cgtcgccgca catcagccgg tccatcggac
      961 gggcggaaca caggtcgtcg atgactgcct ggatggcggc agcctggtct ggcgtctcct
     1021 cgaagccgaa cccttcggca aaggcgtcgt attcatgttg gcgcaactgg aacgcatggc
     1081 cctggcgagc agcgcggcgc gcgtagagat caagcagttc ggcggcggtg tcgcgtgcct
     1141 gttccatcgc ccgccgacgt gctttctccc attggccgct gcccagcctg gacaacggag
     1201 cggattcagc cgctgcacca ctgtagcggg cgatcagatg caggctggcg accgggacgt
     1261 acagtttgtc gccgccggcg tattccagca gcaggaactc catttcgcct tcgcccagat
     1321 ccatgctgca cagaccctga taacgggcga tgccatgttc gacatgcacc accggatcgc
     1381 cgacgttgac ctcggagagg tctcgcagca ggccgtcgat ctgggtgcgg cgcgaacgct
     1441 ggcggccact caccctgact gtgctggcgt agagctcggc ttcggtcacc agcgcgtcgg
     1501 cctgaacgtc ggcaaactcg aatcctgtcg ccagtgggga aacggctagt cgcaagggcg
     1561 ccgcaagcgt ctggcaggca gcaaaatcat cgcacagcac cggtttgagg ccatgctcgt
     1621 tcaggtattg cgccatgatt tcgcgtcgcc cggcgctctc tgccagcagc cacaccggcc
     1681 cctgacgcgt cgccaggtat tgtctgaggc ggaagagcgg atcttcggcg cgacggtcga
     1741 cggcaagctc aggcaaggcg cgcgtgagcg ccggcgcgtc gctctcggcg gtttcaccca
     1801 gttgtaaccg ggcgaacggt ttcatgcggc caaacaggac gtcagtgggc aggaacagtt
     1861 cgccaggcgc gaggatcggc cgttgcggat cgccgcgcat caaccggtag cgggtttcgg
     1921 tatcgcgcca gaattcctgc gttgcggttt cgatatcgcc gtgcaggcag atcgtggcgt
     1981 cggtgggcag atagtcgaac aggtcggccg tgtgctcgaa gaacagcggc aggtagtatt
     2041 cgatgcccgc gggcgccaga ccattgctga catccttgta cagcggcttg cgtgaagcgt
     2101 cgccttcgaa catggtacgg aaattctgac ggaaacgcgc gatgccgttt tcgtcaaagg
     2161 gaaattcacg cgccggcaac aggcggattt cctgcatggg gtagagactg cgctgtgtat
     2221 cgacatcaaa ggtacgcagg ctgtcaattt cgtcatcgaa caggtcgata cggtagggca
     2281 gcgcgctgcc catggggaac aggtcgagca gtccgccgcg cacgctgtat tcaccgggag
     2341 agagcacctg gttgaccgcc tggtagccgg cagcactcat ctgggcgcgc aaggcttcga
     2401 cgtccagctt ctgccccttg cgcaggaaga aggtgtaggc gcccaggtat tccggtggcg
     2461 ccagccgcgc aagcgcagtg gtgaccggca gcagcacgat gtcgcattgc cgcgacgaca
     2521 ccaggtagag cgtggccagt cgctcggaga ccaggtcctg atgtggcgaa aaatgatcgt
     2581 aaggcagggt ttcccagtcc ggcagcaact gcactgacag tgtgggcgca aaccagcgga
     2641 tttcgtcctg cagacggcgt gcgtcgagtg cgtgggcagt gagcacgacc agtggacgtg
     2701 tcgactgtgc gagctggctg atggccagcg catcggatga tccggcgagt ccggacagac
     2761 gcagacgctg accgggatgc ggtgacggga cgtgaatgtt cattgccggg tggcgggtga
     2821 tcaaggcatg caaagcacga cattgtaccg cgaaaccgtc aggacggaag gcttgcgccg
     2881 aatttgccgc aatacgcagg gagcgggagc gggccacgtc ccgccgctgc tgcggacggg
     2941 gcgaggcacg gaacggaagg ttatttggcc ggacaggcgt cggtggaagg caccttgccg
     3001 acgaggagca ggaactggcc gaacggtccc atcacgctca tggcttccca cttggggcct
     3061 tcgaacagca ggtcgccaaa catcatggcg tgcatgggcc cgtattttcc ggcgcccatg
     3121 tcgtgccagt cgtccgtctc ggcccacatc aggtagtcca cgctggagtc gagcgggctg
     3181 gtcatgacct tgccgccata ggcgcacatt gccttgccgt ccttgggcac gatcttgagt
     3241 tcaacctgcg ggctgtcttt gcagtcctta cgataaatat ggatgatctt gtagccgcgc
     3301 ttcttgtcgt tggcaatcca tttgccggcc agaccactgg tcagttgcgg ggtatcgttc
     3361 catgcggcgc aagcctgcgc ggcccactgg ggcgacatca gcggcgcagc cggagcggtg
     3421 tcagccatgg cctggccggc aaatgcggtc aatgccagcg cggcagccag ggcggattgg
     3481 gtgaaaatct gtttcattca ggtccctcca aatattttgt gattcgttat gatgagctcc
     3541 ggccgcctcg acacctgggc cgaagcggga aaggtaccgc cttcggcagc ggctgtcaaa
     3601 cgacccgtcc tgcgggtttc agcacgacgc cagcatgtgc tcgtacgcat cggcaaacag
     3661 tttcagtcct tcggcctgga gcgtttcacc tacctggtcg gggtcgattc ctgtctggcg
     3721 cagccgcatc agcacactct cggcttcgtc catgccctgt tcgacggtgc tggcaacatg
     3781 gccatggtcg cgaaaggcgt ccagcgtggc gtcaggcacg gtgttgatgg tttccggtcc
     3841 gatcaggtca tcgatgtact tgacgtcggg gtaggccgga ttcttggtgc cggtgctggc
     3901 ccagagcagg tattgtggcc gacagccagc gttggcgaga ttgagaaacg cttcgccgtg
     3961 aaacacctgc ttgtacagcc ggtaggtctg cttcgccacg gccagcgcca cccgcccctg
     4021 caggccgctc gcctggggat tggccgattg tgccgccagc agtggatcga catggctatc
     4081 gatgcgcgag aggaacaggc tggcgaccgc tttgagatgt tccgcgccgc cgccgccggc
     4141 caggtgtttc tgcaggccgc ggatgtaggc ggaaaagact tccgaggtct ggcgcagcga
     4201 gaacagcaag gtcacgttga ccgacacgcc atcggaaatc agtctttcaa aggcgataat
     4261 gccagccggc gtggctggca ccttgatcat caggttgggg cggttgaccc gggcacgtaa
     4321 ccggtgcgcc gccgtgaccg tgccctcgac atcgtgcgcc agtgcaggcg acacctccag
     4381 actgacatag ccgtcattgc catgcgctgc gcgccatacc ggaaacagca ggtcgcaggc
     4441 ttcctggatg tccggtacgg ccagcgcctc gtagcgcgcc tcggcgttgg ccagttcgtt
     4501 tttcagccgc gccatgtcat cacggtaata cggactatcg ctcagcgcct tgcgaaagat
     4561 tgccggattg gacgtgaccc cggaaatgcc gtcctgttcg atcagacgtg acagcgtgcc
     4621 ttcgcgcagc agggtgcgtg acaggttgtc gagccagatc gattgtcccc agttcttgat
     4681 gttttccagt ctgctcatgc cgttctcctc aaaagtggaa gcgtcgccgc tgctatggta
     4741 atccgcttgc cgggtttggt cacccccgtt tcgacaacgg tgctcgcctc aggttgcctg
     4801 ctgctatact tgcagcaggt ctgttctgtg cggagcgttc atgccggttt atcccaccat
     4861 cgaccaactg gtcggcaata cacccctggt acgtctgacg cgtctgccgg gcatcggtcg
     4921 caatacggtg ctgctcaagc tcgaagggca gaacccggcc ggttcggtca aggaccggcc
     4981 ggcgttcaac atgattcgcc aggccgaggc gcgtggcgac atccagcctg gcgataccct
     5041 catcgaggcg accagtggca ataccggcat tgcgctggcg atggctgcgg cgatgaccgg
     5101 ctaccgcatg acgctggtga tgccggagca catgagcatc gaacgccgcc agatcatgcg
     5161 tgcctatggt gccaccctgg tacttacgcc gcgggaaggc ggcatggaaa cggcaatcac
     5221 gcatgcgcgc cagatgcgtg atgccggcga aggggtgatt ctcgaccagt tcgccgacgc
     5281 cgataatccg caagcgcact ttctcggcac cgggccggag atctggcgcg ataccgaagg
     5341 cacagtcacg catttcgtca gcagcatggg cacgaccggc acgatcaccg gaaccggacg
     5401 ttatctcaag tcgcgcaatg ccgacatacg catcgttggc gtgcaaccgg aagccggagc
     5461 ggagattcct ggtatccgca cctggtcgca ggcgttcctg ccaaaaatct atgatccgtc
     5521 ggtggttgac gagatccgca tggtcagcca gcaggccgcc gaagacatgg cgcggcggtt
     5581 ggcgcgtgaa gagggcattt tcgccggcat ttcctcgggt ggcgcccttg ctgcggcgct
     5641 ggcactggcc gcagaggtcg aagatgccac catcgtgtcg attgtctgcg atcgcggcga
     5701 ccgctacctg tcgaccggcg tgttccctgc atgacttcgc aggacgaata ctggatgcac
     5761 gaggccatcc agctggcgcg cgcggcggcg actgcgggcg aagtgccggt gggagcgctg
     5821 gtggtgcagg atgaactggt gctcggacgc ggcagcaatg cgccgatcgg ccgcagtgat
     5881 ccgagcgcgc atgcagagat cctcgccttg cgtgctgccg cggcagccgt gggcaattac
     5941 cgcctgccgg gcgccacgct gtatgtcacc ctcgaaccat gcgtcatgtg cgtcggagcc
     6001 attttccatg cgcgtatcgc ccgggtggtg ttcggcgccc acgatccgaa gaccggtgca
     6061 gccggcagtg tggtcgatct gttcggtgaa tcgcggctca accatcatgc gcaggtgcag
     6121 gggggcgtgc tggccgaagc atgcggccag ttgctgagag atttttttgt cagccgacgc
     6181 cagacggcat tggccgcaca atcacgtgcc tgcagcacgg aggcggcgcc ggactgaacg
     6241 ctggcgcggc ttactcgatc acgccacagg ccagtcgtgc gcctccgccg cccagcggcg
     6301 ccggcgaatc ggaatggttg tcgccgccga cgtgtaccat caacgaatga ccgtgcagca
     6361 tgtcaagctg tttcagtcgc ggcgccagca ctggctggtc ggcctgaccg ctggcgttga
     6421 catagagcgg gggcaggtca cccaggtgtc cggtgcccca cggaaaatcg tgccggccgc
     6481 tgtgcatcgg gtcgaaatgc ccgccggcgg ccagcgcagc aaccggcttg ccatctttca
     6541 tcattggagc gcagctgggg ttctggtgca catggaagcc atgcaacccg ggcggcaatc
     6601 cctgcatgtg cggggtgaac accaggccgt accgagtctc atgaatggcg accgtaccga
     6661 ccggcgtgct gacgccctgt gcatccacca ggttcatggg taccacgaga tcggcagcgg
     6721 tagcactcag ggaaacagat agaaccagtg cagaaaaaag acacctaatc atctgatact
     6781 ccttttaaaa caatgcgaaa tcgcacgtca gcatagcatg gcaggcagtg gagtgccgca
     6841 ctttcgtcaa gatggaaaac gaacagcaga agcgtcgtga cgggactgcg tcatcgatgt
     6901 cagcaggcca gccgtgctcc cgctggcgaa aaggtcgcca acacgcatca gccatgcccg
     6961 gatgtctgac atggcaacgt cagagcgctc ccggtggtcg gaagacggga gcgcatttta
     7021 ccgatgactc caccatgcgg acgacaacac gatgcgtaac tgtcattcag ggattgatgt
     7081 cgatcggaat gtccatgtcc agcgtcgagg ccacaggctg gtcattctgc atggcgggaa
     7141 agaaacgcca tgtctgcagg gtcgtcagca cgacctgatt gatgcgcgga tcgctgcaac
     7201 cgtggcgcag ggtgacatcg gcatgcccat cggttccgat ggcaaactgc accgacatga
     7261 cacagtgcag gctcgactgt cgcaacgctt ccgggatatc cggcgtgggt tggtagaccg
     7321 cgcgcgcacc catgccttcg gcaccggggt tgccgccggc gctatcggcc ggggtcggcg
     7381 ccggtggagt gggccgggct ggtggggccg caggtggtga tgcgggtgcg gtgggtgcgg
     7441 gagtcggagc cggtttgacg ggtgcggtcg catgcgttac gggcggcagc ggatgtgggg
     7501 cgacatgggg cgcgggcctc gcgtgcagcg acccaaccgg ccgtgcgggt tggggtgcgg
     7561 gcagttcgat gagacgcgcg tccaccggtg tcggttcggc cgtcggcgct ggcgcttgca
     7621 gcatgcggcc ggccagcagc agcaccagta gccagaatcc gctcgccggt agcaggtagc
     7681 gtgcgaaagg cgtgtgccgg tcagtcatga ctgacagcga tcaggaagtg ctgtgcgccg
     7741 gcatgctggg ctgccagcat cgcctgaacc actgtgccgt gttgtgcgtg attgtcggca
     7801 gcaatgatca ggttgccgtt gctgccgata cgtgcatgca acacgccacc cagttgcgcc
     7861 agcgtcaccg gtatcttgtc cacgaggatg cggttgtcgg ccgtgatggt cagcgtaggg
     7921 gtcttttctg cgctcatgcg ttcggcttgt ccctgtggca ggttgacctg cagggcgtcg
     7981 aggtgctgca tggacagcga ggccagcatg aaggtggcga gcagaaagaa catcacgtcg
     8041 atcatgggga tgatttcaat gcgtcctttg cgctggacgc gggatacgcg cagtttcatg
     8101 gcatcacccc tgctggtcga gacggatgcg gtcgatgagc cgggtgccga gacgttccat
     8161 ttcgtccagc gtctgcgatt gcaaccggga aaaataattg aaaccgaaca atgaaaccag
     8221 cgcgatcatc agaccgaccg ccgtggcgat aagcgcctga gccacgccgc cggtcacgcc
     8281 agtcgggttg acgaggccgt tggcgccgat caactggaag gcgtgcatca tgccggcgat
     8341 cgtgcccatc aagccgagca gtggagcggc agtgaccgtg gtttcgagca gccacaaccg
     8401 ccgcgatagc gtggtttcaa tcacctgcgc ctcgtctgct gcacgcgatt cgatccacca
     8461 ggccgggtga ctgcgattct ctgtgattac attgaaaaac ctataaaaat aattattttt
     8521 attgagattt ttcatttctg aaaatagttt ttcccaatca aactcataac tttccagcag
     8581 gtcggccatc gtttgcggca atcgggtgta ttgccagtag agcacggctt tttcaaacat
     8641 gaccgccaat gcgagtatgg ccagaatgga caaggggacc atcatgatcc cgccgagttt
     8701 gaaagccagc cagtctgctt gcatgcgata cttctcctgt ggtgacgccc tgggcatcag
     8761 gacgattcga aacggtgacg ggtcaaaacg gtttggtcag gctcaggatc accgttcgcg
     8821 tcggcccgta ttgcggtgcg ccaacgccga tgccggtacc gtcgcggatc tcgtagctgg
     8881 tgttgagcag gttgacgatg ctgagccggg catcgagcct gccgacacgc ggcaatttca
     8941 gggtacgcaa ggcacccaca ttaacctgca tgtagccggg caggtggctg gtattggcaa
     9001 aaccactgcg caggccgctg ccatagagca ggtcggcgct ccaggtgatg tcacgggcaa
     9061 accatgagac acccgaggat gccgtccagg tctgatcatg gtcgagatgc acccagttgt
     9121 tggcgatgta tgacagctcg gccggagaga aattgtactg cgaagaaatg atattcctgc
     9181 ccattgcggt gctgttggcc agattgaacc agactgaagt ggtgtcgcgt cggtagctgg
     9241 cggtccattc cacgccatag atcatgccct gggcgtaatt gaatggcgta tacagcagcg
     9301 ccgaaccaaa ctgaccttcg tcgagcagat tggtgacctg cttgtagtag ccatccatac
     9361 cgacattcca gtacggattc agacgctggt cgataccgag gtcgaaatca tcgctcgact
     9421 gcgaacgcac cggtgaattg agtgtgccgc ccggctgggc attggtggtg gactgcgcgc
     9481 cggcgacgag ctggccgctg atgagttcgt ctggcggcgg agtgtaataa tgcgcgtagc
     9541 cggcatgcag cgtggtggcg ggcgaccact ggtataccgc acccagtcgc ggactcatct
     9601 ggtgaccgct ggtataggca tccacctggt agaagcgcag gccgtagtcg atggtgagac
     9661 gttgcgtggc ctgccagcga tcctggacgt acaccccgaa caggttcatt ggcagcgagg
     9721 aatactgcga ctgggcaagc ggcacggtcg aggtttgctg accggcggca ttggcgggaa
     9781 aagccagcag attgttgaaa gcgtctgcct gttcgctgct gatgtaaatg ccggtacgca
     9841 acgtatgcga gggcgagatg cgtgcgtcga aatcggcttc cagtccgttc tcgagtgccg
     9901 tacgcagcac ctgcgacgcg accccggtat agatcaggtc accgggaata tccgggccga
     9961 acagcacccg cgtgtagcgg ctgaatgccg acaactggta atcgtactgg cttccgagcg
    10021 tgccttgcag tgcaagcaac ccgtaacgcg tggtttcgtg ttgctgctcg ttgagtgcct
    10081 gcgagggcat ggtgttcacg cctgacagtg aatacagcgg ggtctgaccg tacagatcgg
    10141 gaatctggaa ccggctgtcg gccgcaccga acatggcgct ggcgcgcagc gtgtcgctga
    10201 ttgtccatga gaggtagcca aacccgttgt tttgcgtcga cgcgtcatgg acggcattga
    10261 acgtcggcat cgggttttcg atgccgagac tgttctgcag tccggaggca tcgagataat
    10321 agctgaaatt gccactatgc ccctgcagtt gcgcggaaga ttcagcggta ttgtggttgc
    10381 cgccgccgat ttccaccgag ccgccctggt cgaatgcacc ggttttggta tggatgtcga
    10441 ccacgccggc agtgcggtag ccatattgcg ccgacagcgt tccggtcagc aggttgacac
    10501 tgtcgatgaa gcggctgtcc agggtctggc cgaaccctga aatcgcttcc ggcagcagga
    10561 cgccattgat gcggtattgc aggtcgccat ggtcaccgcg cacgtgaatc tggccgaacg
    10621 aatcctgagc aacgccgggc gcctgcaaca acagctggtt gaatgaggta tccgtgccaa
    10681 gtggcatttg ctggatgtcc ttgctgctga tgtgatagct gctggctccg ccgttgtcga
    10741 gagaaacccc gttacgcggc gggcttgcct gctgcacggc gacccgcaga cgcagtgcgt
    10801 gtgtgctggc cagtgtcagg cggctggttt tgccttgcgt tccggccagc cagacgatgt
    10861 ctgtgccctg acgatagtcg gtcagggtgg cgatcagcgc atagacaccc gaggca
//
LOCUS       DAIANL010000025        19616 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2471615, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000025 DAIANL010000000
VERSION     DAIANL010000025.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 19616)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19616)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19616
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471615"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4043 a         6328 c         5480 g         3765 t
ORIGIN      
        1 gattgcggcc ggccggtcga acccgaggta tatacgcttg ccactggccg gtcgaacgcc
       61 accgtcgtcc aggaaacacg ctctgttgca atcgctccgg tgaccggcgc gtcgacacgc
      121 accatgcgcg cacccggcaa ccgctccaga tcaagctcgt aatcgagcag gatgacgggg
      181 tcactcaggt cggcgcacac cacagccagg taatccggcg cgctgacgtt aggcagcacg
      241 ctgtgcacac ctcgcacaat cgcggcgaac aacgccacgg caaaccggta acggtcccga
      301 cacagattga tcgcgtaatg cgcttcgggc agtcgtgccg cgagcgcgtg cacttcagtc
      361 aggaacgtgt gccggctgac cggacaccct tcgcgccagg ccaccacggg gtcatcgacg
      421 cactccggca acagcggcaa cgcgtccaca ttcatcgggt cgattcctgc cgacgggaaa
      481 aatcgcgcca tccatggatg atcgccagca catcgagcgc aggacggtcc ggcagcacac
      541 ttcggcgcac caggtactcc gcgacaaaca tcacccccag cagcaccggc gtgcccacat
      601 cggcaaacgt cgaccacacc accagcggcg ccgcaacaaa cagtacaacc gatactcccg
      661 cgctgacggc gaaaaacgcc gtccacgcca gcgtcacgtt acgcgtgtaa cgcaggtaag
      721 ccgcatccag cggttctgca acgagaccca tggcgatccg tgaacacagc gcctgttccg
      781 gttgcccgcc cagcgtgctg ccgaacacga acccgagcag cgtcatcgcc ccggcatgct
      841 gtgcaaaata cagccaggca acatgcgtgc tgaagcgggc ggaatgcaaa acgacccagc
      901 ccgctgccac agcacagcac gccaaccaca gccagcgcat ccgcgcccgc caggctgcga
      961 caagcgccat gctggcgatc ggcatgagcg tgacccacac ggcaagcagc ggcggatgat
     1021 cggcattggt cacgaaataa ctcagcgcga gataccctgc gcccaccagc aaacccgatg
     1081 ccagttgcag gttgcgccgg cttgcggtca tgtggtccga tgcgtggcga tatagtccgc
     1141 caggctgcgc aacgagctga aaatcctgaa attatcctcg ccgtcagcct tcaggtggac
     1201 accaaaacgc ttggagacga ccagcgcgat ttcaaggatg tcgatcgagt cgattcccag
     1261 cccttcacca tagagcggcg cttcgggatc gatgtccccg gccgtgattt ccaggttgag
     1321 tgcggcgacg atcatggcgg ccacctcacg ttcaagcgtg gtatcggccg caggctgggt
     1381 agattgctgt gtaaccatcg aaaacatttt gaatacatgg agaaaagcca ttttatcatg
     1441 ccccgcgcac ttcttgcagg gaatcctgtt tcaatttgtt ttccgggatg gcgcgggaaa
     1501 ctgactctgt gctatggtca cgccaaccca tcttcggcgg acgacctggt ccggccggca
     1561 acctttgcgg agacactgtt gactgagcgt atcacgaagg atattccagg ctggaatgga
     1621 ctggcggcac gggcgctgca cctgttctgg ttcaaatgtt tcggcacggc aggtttcacc
     1681 gccctgttct ttgccgccta cctgtatctg ctcaaacacc cggcctatcc ggtgctgacc
     1741 gtaccgtcca ccgcagccga ccgccttgtc gctttcgaac cctcggtgct gccgctgtac
     1801 ctgtcgctgt ggatatacgt gtcgatcccc cccatgctga tggacagccg gggcgagatt
     1861 gcccgctatg gcatatggat cgggctgatg tgcctggcag ggctgagcat cttctatttc
     1921 tggcccaatg ccgtgccgcc gctgcacctc gactggtcgc gcgatcctgg catcgccttc
     1981 ctgaaacatg ccgacgcagc cggcaatgcc tgcccgtcca tgcacgtggc cactgccgtt
     2041 ttttccgcgc tctggctgca tttccgcatg cctctgctcg gactgcgaca ttggtcgcgc
     2101 ggcatgaacc tgctgtgggg caccggcatc gtctattcga ccatggcaac caaacagcac
     2161 atggccgtcg atgtacttgc cggcgcactg ctggccgcca cgttcttctg gatcggccac
     2221 cgtttgcagc cgtccggaag acgcctgcac cccccagcct gaatccgtcg cccgttgcag
     2281 ttgccgcaac gctataatgt gatcatggac agcgcatcac tgatcgaaaa actggaaacc
     2341 aacggctggt acctggaccg gatgctcggc atgcaccgtc agttcaggca ccctcacaag
     2401 ccggacctga tcaccctcaa gcattccgtg gccgacattc ccgtgcgtac gctacaactc
     2461 gcgttgcggg cagccggttg cccggaaaac accgggtcgg acgcatgaaa tttaccgtgg
     2521 tgctacacag tgaagatggc cagcattatg gggtgaccgt tcccgacctg cccggctgca
     2581 cctcggcagg cagtacgctc gacgacgcac tcgccagcgc aagcgaagcc atcgaactgc
     2641 acgtggaagg actgcttggc ggcggcgggg cgattccgct accgcttgcg gcaagcgcgc
     2701 accagaacga tcctgcactg aaggacggcg tctgggcgct ggtggacgtg gccgtcgaac
     2761 actatttcgg tccggcagaa aaatttcccc tcagcatacc gcgcctgtta ttgtcccaca
     2821 tcgacgagca catcaaacat agcggccaga cgcgcaacga tttccttgtc gaggccgcca
     2881 gcgctgcgct gcgcaaacac accggcgact gagcgcccga ttgccgttca ggccggcaaa
     2941 ccgttctgct cccctgccgc cgaggcctcg cggttacgct gcttgactcg cgtgatacgc
     3001 accacttccg gcagccgacg caaggcgcgc atgatacgcg ccagatgcat gcgctgttcc
     3061 acctgcagcg taaagtacaa cgtggtgtat tgactaccgt cttcttccgc catcgaaaca
     3121 ttatcgatgt tcgatccctc gtcggcaatc acggccgcca gtttggcgag cacaccgcgc
     3181 tgattgagca caatgagctt gatattgaca tcgaacatct tgctcaatcc cggttcccat
     3241 tccacgtcca gccatttgtc cggatcgtcg cggtaatgct tgatggctgc acaatcatgg
     3301 gtatgaatga tcagcccctg gtcggacttg atgaacgcaa tgatcggatc acctggaatc
     3361 ggccggcaac acttcgccaa ctgcactgcg accccttccg aaccgcgaat ggcaatcacg
     3421 ctgtggtggc gctgtgtcgg cagctggtgc tcctgcatca gcaactggcg cgccaccacg
     3481 atattgagcc gcttgcccag gccgatgtct gtcaggattt cctctcgcga cttgccggtc
     3541 tcgcgcgcca ccctgtccca gcaagccggt ttgatggcag ccggatcggc gccaagcgca
     3601 cgaattgcct gctcaagcag ccgttcgccc aaccgcaccg attcgtcggc gtgcatggtg
     3661 cgcaaatagt gacggatatg cgcccgcgcc ttgccggaca ccacaaaatt gacccacgcc
     3721 ggattcggtc gcgcattggg tgcagtcacc acttccacct gatcgccgtt acgcagcacg
     3781 gtgcgcatcg gcatcaattc gccattcacc ttgaccgccg tgcagtggtt accgacgtcg
     3841 gtgtgcacgg cataagcgaa atcgaccgct gtggcgccgc gcgggagtga aaggatcttg
     3901 ccttttggcg tgaacacata gacttcgtcg ggaaacagat ccaccttgat atgttcgagg
     3961 aactcggccg aatcgccacc accttcctgg atttccagca acgattgcaa ccattggtgc
     4021 gtgcgctgct gcacttcgtt gatttcggca tcgctcgact tgtacagcca gtgcgaggca
     4081 accccagcct cggcgatctt gtgcatttcg tgggtgcgga tctggatttc aatcggcgtg
     4141 ccgaacgggc cgaacagcgt ggtgtgcaac gactggtagc cattggcctt gggaatggca
     4201 atgtaatcct tgaatttgcc tggaatcggt ttgtacaggc cgtgcaggta gcccatcgcc
     4261 agatagcacg acggaatgtc gttgaccagc acgcgaaagc cgtaaatatc gaaaacttcg
     4321 gaaaatgcca gcgatttttc ctgcattttc cggtagatgc tgtacaggtg cttttcccga
     4381 ccgccgacga tggccggcag atgcgcttca gacaatttgt gggcaatcgc ctcgcgcacc
     4441 ttttccacga cctccttgcg attgccgcgc gccgccttga ccgcctgtgc cagcacccgg
     4501 taccggttgg ggtacagatg atgaaaacac aggtcgtcaa gctcctggta cagactgttg
     4561 aggcccaggc gattggcgat tggcgcatag atatcgatgg tttcctgcgc aatgcggcgt
     4621 cgtttttctg ccggcatggc atccaaagta cgcatattgt ggagccgatc tgcgagcttg
     4681 atgagaatta cccgcacgtc gcgggccatc gccagcagca tcttgcggaa attttccgcc
     4741 tgcgcatgaa cttcggtctg gaactcgatc ttgtccagct tggaaacgcc atccaccaga
     4801 tcggcaaccg ccttgccgaa ctggtcggag atttcttcct tggtcgccgg agtatcctcg
     4861 accacgtcat gcagcagcgc ggccatcagc gcctgggggt cgagatgcca gcgcgcaaga
     4921 atgccggcca cagcgaccgg atgggagata tacgcctcgc cgctcatgcg gaactgaccg
     4981 ctgtgcgcgg cacggctgaa ctcgtacgcc gcgcgaacct gcacgagatc cgcgggtttc
     5041 aggtaaccca gcccgctctc tatttcttca aatcccggca tgttgcaagt cgccagacat
     5101 tcgcaccaac cgccgctcag ccgcgcgcgc gctccagcac ctcgcgcccc acttcgccag
     5161 cggcgatttc gcgcaacgcc accacggcgg gcttgtcacg gcccggatcg actttgggtg
     5221 catggccgtt ggccagttgg cgggcacggt aggttgccgc cagggtcaat tcgaaacggt
     5281 tgtcaatctt gccgatgcag tcttccacag tcactcgcgc catgccggtt tccttgatgt
     5341 catcatgcgt tcagggacgc aaacagaatc gcatgccgtt gcaactgttc tccggcacgc
     5401 aacctgtgcg cgcgcacgac agccagcagg tcggtcaatg cggtttcgaa atgatcgtta
     5461 atgataacat agtcaaactc gactgcatga ctgatgtcgc tgcgtgcggc agccagtctg
     5521 cgcgcgacgg tcgccgcatc gtcctggcca cgattacgca ggcgctcgcg cagtacctcg
     5581 atcgacgggg gcaggatgaa cactccgatg gcctggggaa acagccggcg cacctgctgg
     5641 gcgccctgcc aatcgatctc cagcagcaca tcgatgcctt ctgcaagccg ctcgcgaatc
     5701 cagcgttcgg aggtgccata gcgattgcca tacacttcgg cactttcaag aaactccccg
     5761 gcttcgagca tggtcatgaa ccgcgtctcg tcgacgaaat gataatcctg tccgtcgcgc
     5821 tcacccgggc gcggtgcacg tgtggtgtgt gaaaccgaca ggcaaatctg ccggtcgcgt
     5881 tcgagcatgg cgcgcacgag actggtcttg cccgcacccg aaggcgccgt cacgatccat
     5941 acattgccgg tcatttgctt atcctgactc ccagatgaaa aatcgacagc gacaacgcct
     6001 gacattatca tggattattt tgccactgca gcgcgtctac cccataccaa ccaccagcca
     6061 aggagcatcc cgatgaaaac cggcatttcc cgcatactgt tgacgctggc attcagcagc
     6121 ctcgcattgt gcagcgtgtc tgcccaggca gatgacgaca gcttcgcccc cggcggcacg
     6181 gccatcgatc accacgacga acgttacccc aacctgaaag tggtcatgga cctgaaagta
     6241 cgcatcccgg ctgatgtttc gttcggtgtc gctaccgcaa gacggattat ctcatacccg
     6301 aacgccaagc tggtggtcgt catcgagggg ccttccgtgg cgattttcgc caagaagaac
     6361 tatcttgatc accagggcat ggtcgaccag tgggcggacc tcgccaacaa gggtgtgcag
     6421 gtggaattct gcggcaattc ggtgcatggc gccggactca agcctgccga catggatggc
     6481 ctgagcgaca agaacccggc cgtggtcaat cccggcgcgc taccgagcat cgcccattac
     6541 gaacagatgg gctacgctct ggtcatcccg agcccgcagg aacagccggc atcataacca
     6601 cagtggccgc gttgcctgag cggctgtcgc aacgagggca gccgcgcggt cgcgcaccta
     6661 gtcgaagggc acgccgatga tggctttgac cacccagttg tcggacaccc cggtcgtgcc
     6721 ccgaccaacg ccgaagttca gatcccagcc gtgcaccgaa gtatcgatca cggcataggt
     6781 cacctggctg aactgaccga acgatccggg atcattggcc gcccccagcc cattgtagct
     6841 ctcgatgccg atctgcgtgt gcgcagcgat cgagtagccg atcttgctgt ccagctcgaa
     6901 cgtggccggc tcgggcaccg gaccggacac aacccagtcg tagttcaggt tggccgccag
     6961 cgtccagcgt ccgatccggt cgccaatgat ccccttgagt tcggcattcc acggattgat
     7021 gtcgagcaac tggctcacgc gaccgatctc gaaattgccg ccccagaacc aggtctgatc
     7081 cggggtgcgc ggtgcaatgt atttgatgcg gaatttctcg ccatcgacct gggttccggc
     7141 ctggacatcg gtggtggtca ggaggtaggc gcccagttcg atatctggtg tccaccccca
     7201 ggagaattcg ggcgtgacgc ggaacaggtg cgccgaaggc aactggccgg catactgcgg
     7261 cggcacgctg ctggatggca catcattgac gtgaacatcc aggccgaact gacccggcgc
     7321 gtccatctcg tccatgtaca cctggatttc ttccggcgca gcccatgcgc cggaacccac
     7381 acacaacagc caggccagcg ccaccagcct gtattgcaat gaaaccggcc agttaccgga
     7441 tcggccggaa tgtatttcag tggtcatgct ggttgccagt ctccgcaaac aggtcggtca
     7501 tgctgttcac gccggcatcg caggcatggt tcaggcactg gagcccgaac gcctgttcca
     7561 tcgatttgag cagggaaaaa tgactgtatg gccggtgact gcgcagacca tgcgcgccat
     7621 agctggtatc caccaccatg accacccgat tcggctcgac acccgaactg ttctcgtccc
     7681 acaccagcac aatggcgttg cgcccacgct tccataccgg cgaagcatgg atcgactcga
     7741 ccaaccgacg cacggtggcg tcacccgcac gcaccaggtc ggcattgtcc tcgcactgcc
     7801 tgccggcatt gtccatgcca tgcatgtcat ggcactgatc gggcacgatg aaggacaacg
     7861 ccggcacctg cccgtcggcg aggtcggcat acagcccgtt catgccacca aacccggaca
     7921 cactggccag actgtccgct gcgacatggc cgccctgcac actggcgaaa tagatgaaag
     7981 gatcatgctt ggcggcgtac agccggcgca acccgccggg gatggccagt gcctcagcgt
     8041 ccttgttggt aaacacccca tcgctgtaat ccacgccatc gactgcgccg gactccggca
     8101 cactctgctg gtaggttttc cagcgctgcc catgcgccac gagctggtcg ccaatctgag
     8161 tgccgcgata gcgcgcggcc ggcagggcca cgccaaacgg cgccatgacg gcgatggtcg
     8221 gcaaatcggt gccttcgccc gcaatcggct cattgcggtc cttgcccggc attgcgccat
     8281 gccaatcggg ataattgtca ttgtggatgc cgaaattcga gccgccgacg atttccagat
     8341 aattggtcag gctcggatgg cccacggcga aatagttggt ggcaagattg gccgtacggg
     8401 cgaactggtt gatgtatggc gcattgcgat tgccgatgat gtcatcgtaa gaacggtttt
     8461 ccatcacgat cagaaaaacg tgatcgagtg ccggcatgcc gccggccagt gccgcctgtg
     8521 tacgggcagc ggcgacgaag gccagccctg cggctgccag cagtctgcgc atagcgtgtc
     8581 tcatgtccat atcctcgtct gaatgaaggg gggtcgatgc actctaaccg atacacatga
     8641 cggatttgtt gcaatcgtgc cggtccactt tcagacaatg atcattcaag attctgcacc
     8701 tgttcgcgca tctgttcgat gagcaccttg agttcgagcg ccacattggt gaccgctgcc
     8761 gcgaccgatt ttgacgccag cgtattggct tcgcggttaa gctcctgcat gagaaaatcc
     8821 agcggcttcc cgaccggacc accgcgatcg agcaatgctt cgaccgcctg caggtgggcc
     8881 gccagtcgcg aacattcttc atcgacatcg gtgcgctggg caaacaggac gagttcctgt
     8941 gcaatgcgct cctcaccggc gctgacgacc acctcggcga agcgccgggc aagccggttc
     9001 cggtattccg ccagcacccg cggcagatgt gtccccagtt ccgccagccg cgcacggatg
     9061 tcttcgacac gctcgcgcag gtgctgtgcc agcgccgcac cttcgcgacg ccggctaagt
     9121 tgcagcgcca gcatggcttc gtcaagcagt ttcaggccgc acgcttccag cgccgtcgca
     9181 ctgccggcat cgagcatgcc tggccagcgc agcacatctg ccaccgacag cggagcagcg
     9241 gcgggaattg cctgcgccac ctgacccgca accccggcaa gcgcgtacag caacgcctcg
     9301 ttcagcgcca gcgacggcgc ctgggtcgcg cctgactgca ctgcgatgcg gcactcgacc
     9361 ttgccgcgca ccagttcgcg ctgcagccgc tcacgcagtt gcggctccag cgaacgcact
     9421 tcatctgcca gacgaaaatg gatttccagg tagcgcgagt tgacgctgcg cagttccacg
     9481 gtcacccgct ggccaccctg ttccgcagcc acgctggcgt aaccggtcat gctgctgact
     9541 acagactgca tcgacttccc cacctcatca gacatgcgca atgttacggg gattggctgc
     9601 gtgcgccaag cgccttgccc cggcacactc gcccggacat gcgtattcag gcatattcgt
     9661 ctccgcgcga cgtacaattc tgtgttacag ttggcttcag ccgggatttc tgcgcgacac
     9721 gtcgtcgtgt tgtctgcgaa cgcatgttat gaccacccaa ccgaacaacg ccctgcccag
     9781 cggctaccaa ctgatggatt accgcatcgt ccgcaagatc ggcggcggcg gcttcagtgt
     9841 ggtctatctg gcctacgaca gcaaggacca ggaagtcgcc atcaaggagt atctgccggt
     9901 caccctggtc acccgcgacc acaaccactg tgtggtgccg ctatcggaaa gcaacttcca
     9961 gtcgttccgt tatggcatgc gtttcttctt cgaggaaggg cggtcgctgg cgcgcatcat
    10021 gcaccccaac gtggttcggg tggaaaactt tttccgcgcc aacgacacgg tctacatggt
    10081 catgcgctac gaacgcggca aaacgttgca ggaatacatc cgttcgcatc gcggcgaagt
    10141 caaggaatcg ttcatccggc gcatcttcgc cgaactgctc aatggattgc gcgaggtgca
    10201 tgcgcaaaaa ctgctgcatc tggacatcaa gccatcgaac atttacatcc gcatggatgg
    10261 ctctccggta ctgatcgact tcggtgcggc gcgtcagtcg ttcaacgccg aacatcagaa
    10321 actggtcacc atgtacacgc ccgggttcgc cgcaccagaa cagtacaagc aaggtaccct
    10381 gcagcagctc ggcccgtgga ccgatattta cagcgtcggc gccaccatgt atacctgcat
    10441 gtcaggcatt gcgccgctgg ctgccgacgc ccggctggat cacgacaagc tgattccggc
    10501 ggccaaggcc tggcggggaa agtactcgcc ggagatgctt gaactgatcg actcctgtct
    10561 gcaactcgat ccgctcgcac gtccgcagag cgtattcaac ctgcagaagg ccattatgga
    10621 aaccagcgat ctcgaggttc gcccaccggg tttgctggaa agcatccgca acaagctgta
    10681 caaggatatt ttctgattcg gggccgctta tgcgattttc catctatcag gccagccgac
    10741 aaggcgggcg caactacaac gaagaccgga ttgcctattc ctatagccgc gatgcgctgc
    10801 tgatggtcgt ggctgacggc atgggcggcc acagccacgg cgaaatcgcc gcgcaactca
    10861 ctgtaaaaac gctgaccgaa gcattccagc gccatgccca ccccaaattg cgcgacccgt
    10921 ccggattcct gcatggcgcc atccatcgcg cgcacgaagc catcaaccag tatgccaccg
    10981 aacaacgtct gaacgaggcc ccgcacacca cctgtgtggc gtgcgtgatc caggacgata
    11041 ccgcctggtg ggcgcatgtc ggcgattcgc gcctgtacct gttcagtgac aacaatctca
    11101 ttctgcgcac ccatgaccat tccgcggtgc agcagatgtt cgacgaagga aagatcagcg
    11161 aagccgagat gtcggagcat cccgaccgca acaaggtcta caactgcgtc ggcggctatc
    11221 tggtgccgga aatcgagctg tcgcggccga tgccgctcac caagcaggac cgcatcctga
    11281 tatgcaccga cggcctgtgg ggtcaggtta cgaccgaaca gatcgccacc acgctgtcgc
    11341 gctttccgct ggcgcgcgca gtcgaatttc tcatggatca tgccgaactg cgtggcggcc
    11401 gtcacggcga caacctgtcc gcaatcgcgc ttacctggga atcaccggac gaatcggaag
    11461 actacacagt ctcgaccgta gccatggcgg aaaacacctt taccaccaag atgagcagtt
    11521 acacaccgag cccggccgac ctcgccgaag aggaggtcga gcgggtgatc gccgaaatcc
    11581 agcacaccat ccgtaaattt tccgacgaga acccatgacc cgcattcatc gacccagcat
    11641 gcgcgctgcc gatcagttgc gcgacgtcac catccagcgc aacgtgctgc gccatgccga
    11701 aggctctgca ctgatccgct gcggcgatac tcacgtgatt tgcactgcca gcgtcatcga
    11761 aaacgttccg gcatttctca agggcaagaa ccagggctgg ctcaccgccg aatacggcat
    11821 gttgccgcgc tcgaccggca cccgcatgga ccgtgaggcc gcacgcggca aacagagcgg
    11881 ccgtacccag gaaatccagc gcctgatcgg ccgggccctg cgcgcagtcg tcgatctgtc
    11941 gctgctcggc gagcgcaccc tgcacagcga ctgcgacgtg attcaggccg atggcggcac
    12001 ccgcaccgca tcgatcactg gcgccttcgt cgccacacac gatgccatcg gcgcgctgct
    12061 cgccggtggc aagcttggcc gcacaccact gcgcgatgcc gtcgccgccg tatcggtcgg
    12121 cctgtggaac ggtcaacccg tgctcgacat ggactatgcc gaagacagcg gatgcgacac
    12181 ggacatgaac attgtcatga ctggccaggg ccatctggtg gaaattcagg gcactgcaga
    12241 aggcacggca ttttcgcgtt cacagcttga ccagttgctc actctggccg aaagcggcat
    12301 cgcccggctg atcgaaatcc agcgcgcaag cctggcacag ccatgacccg ccaggtggtc
    12361 ctggccagcg gcaatgccgg caagctgcgc gaaatcgagg cctggcttgc accgctcgac
    12421 taccatgccg tggcgcaatc gcaactgggc gtcagcgaaa ctgcggaacc acacgcaact
    12481 ttcatcgaaa actgcctggt caaggcacga cacgctgcac gtctcaccgg gctgcctgca
    12541 ctggccgacg actccggact gtgtgtgccc gcgctggatg gcgcccccgg cgtgcactcc
    12601 gcccgttatg cgggtgaagc cgacccggcg ctgcgcgatg cgcgcaacaa ccagcgcctg
    12661 ctggccgaac tggcgcaatg cggccagcgc gcggcctggt actactgcgt catggtatgg
    12721 ctgcgccacg ccgacgatcc gcagcccctg atcggcgagg gcatctggca cggcatcatc
    12781 ctgtccgagc cgatgggcga tggcggcttc ggctatgatc cgctgttcct tgtgcccggg
    12841 ttcgaccgca ccgccgcaca actgaccctc gaagaaaaaa atgcggtcag ccaccgtgcg
    12901 caggccctgc gagccctgtt cgaccgcctc accgcacaat gaaccgcatt ctgcttccgc
    12961 acgagtggcg cctgaccgct tcacctccgc tgacactgta tgtgcatttt ccgtggtgcg
    13021 tgcgcaaatg tccgtactgt gatttcaatt cgcataccgc acaggatgcg gtaccagaaa
    13081 cccgctacat cgacgcattg ctcgccgatc tcgaacaggc attaccggcg gtctggggcc
    13141 gtccggtaca cgcgatattc atgggcggcg gtacgcccag cctgttttca ccggcatcga
    13201 tcgaccggct gcttgccgcc atccgcagcc tgctacctct cgatccgctg gccgagatca
    13261 cgctggaggc caatcccggc acgctggaag ccggccgctt ccgcgaatac cgttttgccg
    13321 gcatcaaccg gctgtcgctg ggcatccaga gtttcaacga ccggcacctg acggcgctcg
    13381 gccgcatcca tggcgccagc gaggcgcacg ccgccatcga actggctgcc atgcatttcg
    13441 acaacttcaa cctcgatctg atgtacgccc tgccgggtca gacgctgtcc gaatcgataa
    13501 ccgacctcga cacggcgctc gcagccggcc cgccacacct gtcgtgctat cacctgacac
    13561 tggaacccaa cacgcccttc tggcaccatc cacccgacct gcctgacgat gacctggccg
    13621 cagacatgca gcaggccatc gaacagcgcc tcgaacacgc cggctacctg cattatgaaa
    13681 cttccgcgtt ttcccggccc ggccaacatt cacgccacaa cacgaattac tggcagttcg
    13741 gcgattatct gggtatcggt gccggggctc acggaaaact gtcgtcccat gatggcatcg
    13801 tccgcgaact tcgtcatccg cacccgcgca attacatgga acagaccgag gccggcaacc
    13861 cgctgctgca gcgtcagcca gtcaggcacg acgcgctggc gttcgaattc atgatgaatg
    13921 cactgcgcct gtcacaggga tttcatccgg cactgttttc cgaacgcacc ggcctgccac
    13981 tgatactggt acaggcgcag ctcgacacag ccatccggcg cggcctgctg cagcaggatg
    14041 acgcacgtat tgccccgacc ccgcaaggcc gacgttttct caatgatctg ttggcgttgt
    14101 ttttgccaga cgcccgggag cagccatgaa cctgccgctt caattcgaac gcaccgccat
    14161 cctgcttggc gaggctgcgc tgcgccacct gtccggccgg catgtactgg tcgccggatt
    14221 gggcggcgtg ggcgggcagt gtgcggacgc gctggcccgc gccggcatcg gccgcctgac
    14281 gctgatcgac agcgatgtca tcgttcccag caacatcaac cgccagttgc cggcattgct
    14341 gtccactgtc ggcaaaacca aggtggacgt ggttcgcgca cgcatcgccg atattcatcc
    14401 ggactgccat gtcaccacac agcgcgtgtt cctcactccg gaaaacatca gcggatatct
    14461 ggcggaagat ctcgatttcg tgatcgactg tattgatacg ctggcgtcca aggcagcact
    14521 gatcgctgca gcccgccagc gtggcctgcc cgtcgcatcg agcatgggcg caggcaaccg
    14581 gctcgatccg gctcgggtgc gtctggccga catcagccgc acccatggct gtccgctggc
    14641 gcgactgctg cgcgcacaac tgcgcaaact cggagtgcat gatggcgtgc tgacggtatt
    14701 caccgatgag ccgccgcgtg cgccccgccc gcgtggcgaa gatgagtcac gccccaccaa
    14761 cggcaccatc agctacatgc caccgctgtt cggtctgatg ctggctggtg cggtggtgca
    14821 acggctgctg cccgagacat gactgcgccc gtcaagcccg cgacgcctgc cgtgtcctgt
    14881 caggcgcgcg acgagcgcag gtggttcttg agtttgcgtg ccaggttcgc cgcatcgcgc
    14941 agcaatggcg tacggtcgat ccacgacaac gggtgaatat cggttttcga actgcgttga
    15001 tagcccagtt ccggcacggc aatcagggtg agcatttccg gatgttgccg ccggaaccat
    15061 gaataggcgc catcgacatg catcggcccg ccgtccggat gcccccccgg acgagccagc
    15121 atggcctgca gatatgcagc ggcacgttcc atgaccggtg cctggaaggc gacgaaatgc
    15181 gtggtacgga ccgcagcctc ggccgccatg gactgaattt ccgcatcagg tcctgcgagc
    15241 ggttgcagca attcgtaacc gccataaaac accccccagt cggactgttg caggcgggca
    15301 cacagggctc cgatgcgcgc gttgaaatca cggctgaaat caagatcgtc ttcaggaatc
    15361 aggatccgct gcaaaccctg ctgcgcggca tcctgcaaga tggccagatg gctggaaaaa
    15421 cagccacgcg ccccgatact gggaaattcg ccggcagcgt tcggacggat cgcggggaag
    15481 aacatcacgc gcggatgacc acccagcccg accttgacga gctgatcttc catctcgcga
    15541 cggcggtcgg tgcgctcggg cagattgacg atgtaaatgc gatcgaacag ttcggcacgc
    15601 cacctcaata ggcattcttg tcggcgagct tcttcagcgt accgagcatg atcgacagat
    15661 caagccagat cgaccagtgg ttgatgtatt cgatatccga ctccacccgc tggatcatga
    15721 gattgatatc gcgcgtttcg ccacgcagcc cgcgcacctg tgccagaccg gtcatgcccg
    15781 gcttgatgtg caaccgggca aaatagtcgt tgatacgacg cgaatactcc gcatcatgct
    15841 gaatggcgtg cggcctggga ccgaccagag acatgtcgcc ggaaagcacg ttgaagattt
    15901 gcggcagttc atccaggctg gtacggcgga tgaatgcacc gacacgagtc acgcgcggat
    15961 catttttttc cgcctgcttc acggttgcgc cttcctgttc cgattgcaca tacatgctgc
    16021 gaaatttcca gatgcgaaac accttgccgt tccagccggt gcgtttctgc cggtagaaca
    16081 ccggcccggg gctgtccagc ctgatcagca cggcgatcgt cagcagtatg ggcgcgatca
    16141 tgagcaggat gatgctggat agcagcatgt cttccatcag cttggcaatc cggttcagcc
    16201 ccaccagcgg tgtttccgac aaggtcagca ccggaatgcc ggacagttcc gacacactgt
    16261 gattgaccag ccgcagcgag aaaatatcgg gcacccagtg gatggtgatg tatttgtcga
    16321 gcaggaactg atagatgcgc ggcagaatcc ccgaggcgtc gagtggcacc acgatgtaga
    16381 caatgtcgat ctggtgctgc gccaccaact gcggcaggtc gccaatacca cccagcactg
    16441 gcgactggct ggcttcagta tcgtattccg catgctcggt cagcgaaacc atgcccacga
    16501 cgcgctcacc catccacagg ttgctggaga tcttgaaccg gagaaaatcc gcaagcgcgc
    16561 cttcaccgat gatcaggacg ttattgacct gcggcgaatt gcgcaccagc gcgtggtgca
    16621 ccgacttgac cgcagcatga aaccccagtt gcgcgacaaa accgatcacg tacatctcga
    16681 gcaggaacag ccgtgaatag tcttcgcctt cgcgtgacag gtagaacagc ccgaccagac
    16741 cgaggaaact gaccgaccag gcctgaaaca gcgccgcagt cttgctgatg aaatcggcat
    16801 tgttgcgata aatgccgaaa tactcatagc tgattgccat gatgcccgtc agtaccagca
    16861 gcatgatggc gtcgtccgac gtcatcgcgc cggtccgata gatgatcagc atgcgcagga
    16921 tggcgaacac acccacataa tccaggcttg cctgcaacag tttgaccaga ttgcttcggc
    16981 gacgaagcaa cggttttttg tgcttcacat aaaacgacat agaaaaatac ctgtcaaccc
    17041 ggcttcgccc gcccggtatt caaatggcgt tgccgcaccg attcagtgcc tgcaggcgcg
    17101 acgacacccc aagcttgtcg aaaatccgct tcaggtgatt cttgaccgta ttgggactga
    17161 tatccagaat cgcaccaatt tcatgattac tcttgcccag tttgacccat ttcatgatct
    17221 gcttctcgcg ctcgctcagc accgccacgg cgcgactgca ctcgtcatcc accacgcccg
    17281 cttcttcagg cggctgattg tcatccacca gcatctcgat acgccgcaac gccagatcga
    17341 tatgcggcat cagcatttcc agggacaacc ggcgcaacgg gaattcatcc gcagcatcga
    17401 aaaaaacata caggctgtca ttgtgaccgc gcaggtcatg aataccatac accagcaggg
    17461 atttcatgtt gtgcaaccgg tccatgaaac attttgcacc gtttgctggc acggtcacca
    17521 cactatcgag cgaggaaaaa cggttcatga cgaaccactg cgcatcctgt tgctgccatt
    17581 tgccaaacag gctgcccatc aacgcatcga tattctcccg tgcatcgatc aggtgttgcg
    17641 tacgcacttc ggaaatactg gaaatcacgt cgtaattcag cctgccgctg gaaaaattgc
    17701 cccatgccgc aatcatcacg tcatgcggca tcatttcgcg cacgctgccc tgcatccaga
    17761 gaaacaagtc cacgtgccgc gtgatggcta acgactgctg caccagttcc tgcagccgat
    17821 cggatgaaac tgaatccggt ttcatcattt cattgactga caaaccatgc cctcccggaa
    17881 tagttgcatg atacacctgc gggcggcaac aaggcttcaa accctttcat tttcctgaag
    17941 cccgtttgtg cgacgcagaa cgcgccgttg cccggcgcat cccctgaacc caccctgaaa
    18001 ccggccctta taattttgcg gccgacagat gcaaagtgca attccagtta tttctataaa
    18061 gatttccgca accatatcat tttccgtata tctttaccac aactggattg cagcatcatg
    18121 tcaatccgga aaaatagcac gccgccactt gcaaacaact tacaggctat tgcacgcata
    18181 ttgcagcaaa ctgcatgcca accaaacgac ccggcagcag caccgggtca ggcgcatgtt
    18241 ttcataaggt ttccgcacaa accctacata aaccgaccac ccgaaatgac tattgcgcca
    18301 ctgcttgcag taattagaat gggaaccgtg agagaaagcc ccggcgactg actctcgtgc
    18361 agaggtaacc atctgatctg tcagtggtta cggacgcgga ctgaatggga gaggagcgct
    18421 ggtactggct tacgcccgga gagtcggatt ggatgagaag gtttgaaccg cacagggtcg
    18481 aggagtactt gacagtctgg cgtaccgtct caaggaaaag cagcattttc ccgccacagg
    18541 aacagaaaca gtggccagca acagcaggat ttggcagttt tttatttcga atttatttta
    18601 tctggaggtt acgccatgcg tcactcgatt cttgcagcgg cagttctgac tgccctcgct
    18661 tccgtgccgg cctttgccag cccgattccc ggcctgtaca acactggtgt cgacaacagc
    18721 ggcaatctgc tggcagttgg cgctcccgac atgcactatg cgttcaacgt gctcagcggc
    18781 accgcccccg gcacgaacgg ctacggcgtg gttgccaatc ccacgttgta tccgatgtcg
    18841 caaaatgact gggtgcccaa caccagcgtg tcgcagtggg tgacgccgaa tgcgaaccag
    18901 ccgaccgaag atccggttgc caacggttac tacacctgga cgctgacctt tgacctgaac
    18961 ggcaaggtca accccaacag cgccagtttc gctgcccagt gggctgccga caacgtcggt
    19021 tacgccaccc tcaacggcaa cctgatcagc ggttccaaca ccggaaccgg tacgcaggcc
    19081 tttggcacct ggcacgactt cagtgcgaat tccggcttcc agtcgggact gaacacgctg
    19141 acgttctttg tggaaaacct tgcccagtca tcgtccaacc cgaccggtct gcaagtggac
    19201 ttcaccagtt ccagcatcac cccgaccccg atcccgggtg ctgcgctgct gttcatctcc
    19261 gcagcaattg ccggtggtct ggtgttcggt cgcaagcgca aggacgcgca gggcttcatg
    19321 ggtgccatgg ctgcctgatt gccggcacga cattcctgct gaacgaaaag gggggctttg
    19381 cggctccctt tttcatgtgc cgttcccgca gcaacaccgt agcatttctg ctggcgttgc
    19441 cgatgcgccg cacacaaaac acgctataat caccaggctg gcatgccaga ctacgccaga
    19501 cgctggttgc ttcactgttg gaggcctgat gaaaaccgat cgcttcatcg ccagatgcct
    19561 gtttgcgttt gccctgcttt gcaccctgat gtgccaagcc gcagtggccg ccgaac
//
LOCUS       DAIANL010000026        29942 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2882158, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000026 DAIANL010000000
VERSION     DAIANL010000026.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 29942)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29942)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29942
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2882158"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5847 a         8321 c         9644 g         6130 t
ORIGIN      
        1 accactacag aaatcggtag taatgagcaa tacaacttcg acattgtcag acgattttcg
       61 ttcacggcag tgttgtgggg gctgatcggc atgtcgatcg gtgtttggat tgcatcggaa
      121 ctggcctggc cgttcctgaa ctttgacatc ccgtacatca ccttcagccg cttccgtccg
      181 gcgcatacca cgaccgtgat ctttggcttt ggcggcagtg ctctgtttgc cacctcctac
      241 tacattgtcc agcggacctg tcaggcgcgc ctgatcagtg attttctggc gaactttacg
      301 ttctggggct ggaccgctgc cattgcgctg gccgatgtca gctatatgct cggatatacc
      361 cagtcgcgtg agtacgcaga aatggaatgg ccgatcgaca ttctggtgga agtggtctgg
      421 attgcctaca tggtcgtcta tatcggcacc atcatgaagc gcaagcagcc gcatatctac
      481 gtagccaact ggttctactt gtcattcatt ctggcaactg cactgctgca caccttcaac
      541 aatctggcaa tccccatcag tctattctcg atgaaatcct acagcctgtt tgcgggtacg
      601 caggatgcga tgactcaatg gtggtatggc cacaacgctg tcggcttctt cctgaccgca
      661 gcattcctgg gcatgatgta ctactttgtg ccgaagcagg ccggtcgtcc gatttactcc
      721 tatcgcttgt caatcgtcca cttctgggca ctgtcgttcc tgtacatgtg ggttggcgca
      781 caccacctgc actggaccgc tctgccggac tggacgtcga ctctggccgc aaccttctcc
      841 atcatgctgc ttctgccttc ctggggcggt atgatcaacg gcatcatgac cctgtccggt
      901 gcctgggaca agctgcgcac tgatccggtg atgcgtttcc tgatcgttgc cttgtcgttc
      961 tatggcatgt caaccttcga aggtccgctg atggcgttga aggatgtgaa cgcgctgtcg
     1021 cactacaccg actggacgat tggtcacgtg cactctggtg cgctcggctg ggtggccatg
     1081 gtgagcttcg gttctctgta ccacatgatt ccacgcatct ggaacactaa aatgtggagt
     1141 gaaaagctga ttggcatcca cttctggctg gcaaccatcg gcgtactgct gtatatcacc
     1201 gccatgtgga tttccggtat cacccagggc ctgatgtggc gtgcgtttga ccagtacggc
     1261 aacctgcagt attcgttcgt cgagtccgtt gcagccatgc acccactgta cgtgatgcgc
     1321 gcaattggcg gtatgttctt cctgtccggc atgctgctga tgttcattaa tatcatgatg
     1381 accatccgtt cgggtcgtca ggcaccggca tacattgcag cgcctggcaa cgtgtctgcg
     1441 gccagtgcct gattgaggga gataatataa atgttcaagc atgaatggtt ggaaaccaat
     1501 atcggtctgc tcgcagtagc gaccatgctg gccatcagta tcggcggtct ggtggaaatt
     1561 gcccccctgt tcttcatcaa gggaacggtt gaaaaggtgg atggcgtacg tccgtacacc
     1621 ccgctcgaac tgcgcggtcg cgatatctac caacgcgagg gttgctacct gtgccattcg
     1681 cagatgatcc gtccgttccg tgacgaagcg ctgcgttatg gccactactc gctggctgcc
     1741 gagtcgcagt acgaccatcc gttccagtgg ggttccaagc gtaccggccc tgacctggca
     1801 cgtgtcggca agaaatattc caacgaatgg atggtgcagc atctggtcag tccgcgttcg
     1861 cttgtgccgc agtcggtaat gccgaacttc ccgtggttgc tgaccacgca actggattat
     1921 tcggatatca aggagcgcat gcgcgcactc cgtgtcaccg gcgtgcctta ttcgctgacg
     1981 aaggccgaat acgatgccaa cgtgaaaaaa tttggcgcgg ataccgccaa tatgctggac
     2041 atcaaccagg ctgagaaaaa cctcgtcaaa caggcgcagg acggtaacta cgatggggat
     2101 cgcagcaaca tttccgaaat ggatgcgctc gtggcctacc tgcaggtgct gggcacgatg
     2161 gtggatttcc gtaagtatga cgacgacgag ttcgtcaagt accgttaact gggaatgttc
     2221 gcggaatggg atggatctat tggttttctt cgtatgagca caccaagccg ctggcgctgg
     2281 tgatcctgtt cgtaaccttt gtgctgatcc tgctgtacgt gtatggcagc aagaagcgtg
     2341 gcgagaaact ggaaagctac aagaatatcc cgttccttga tgacgaatcc aaaccgaggg
     2401 aagacaaaaa atgagtgtga gcaaacagca agcgcacgtg ccgcagacaa ccgggcacgt
     2461 ctgggatggg gatttgcagg aatacaatag tccgcttccc aactggtggc tgatcgcgtt
     2521 ctatacgacg gtagtgtttg cgctggtgta ctggttcatc tatccggcct ggccgattgg
     2581 cggatcgtat accaagggtt tcgccaccat cacctatacc aatgacaagg gcgaaacgaa
     2641 gaccgtacca tggaatacgc gcgccttgct gatggcagaa acgaacaagg ccgaagccat
     2701 gcagaagccg tacttcgaca agattgccag cacgcctttc gagcagattg ccaaggaccc
     2761 ggaactcaac agtttcgtgc tttccgctgg caaaacgctt ttttccgata actgcgcacc
     2821 ctgccaccag caaggcggcg cgggcaaggt tggcttcgcg cccaacctga cggatgacga
     2881 ctggctttat ggcggccatt tcgcacagat ccagcagacc atcaccaatg gtcgccacgg
     2941 ttacatgcct ccgtttgcca cagtgctgga cgacaagcaa atgaccgaac tggccgacta
     3001 tattctcagt ttcgagaaga ttccggtgga tccggccagt gccaaggcag gcgatgaact
     3061 gttccattcg gaaacggctg cgtgctacta ctgtcatggc gccaacgcaa aggggcgtca
     3121 ggtgattggt tcggccaatc tgactgacag catctggctg tgggccaacg tgccggacgc
     3181 aaagacggtc gcagacaagg aagacgccat caagaccgtt atccgtggcg gcctgaacaa
     3241 gggtgtgatg ccgacctgga aagagcgcct gtcgccggac cagatcaagc tgcttgccgt
     3301 gtatgtgcat gacctgggcg gtggtcaata atatttttcg actcgaattt ctgacataat
     3361 cggttatgcg agcctgaccc ccgcccgtgc gggggttttt gtttggagaa tatcgtggaa
     3421 tctcaagctg aagaacatct ggatctgtac gaaaaacgta ttccgattgt gacgcgctgg
     3481 gctcatggca agtttagaaa cttcaaatcg ttgatgttgt ggctggctta tgcggtattc
     3541 tttctgctgc cgtggctgcc ctggcaccgt cacagcggta tcaatcaggc cgttgcattc
     3601 gatctgatcg gacgtcgctt ttttattttc gatctggtgg tctatccgca aaatgtattc
     3661 tggctggcct ggctgctgtt catcgcagcc accttcctgt tcttcatgac gactctggtg
     3721 ggtcgggcgt tttgcggcta tttctgcttc cagacattgt ggaccgacgc gttcatgatg
     3781 attgagcggt tggtgcaagg cgaacgaccg gcgcggctga aactcatcaa gcagccgtgg
     3841 aacggcgaga aggtcatcaa gattggcatc acgcacacgc tgtggctggt cgtgtccttc
     3901 tggaccgcac tgacgtttgt gctttatttc ggctatgcac ctgaattgct gcgccatttt
     3961 tttaccgggc aggcagctga agccgcctat atcggcgtgg gcgtgctcac gatgaccacc
     4021 tacattgccg ccggattcat gcgcgaacag gtgtgtgcct atatttgccc ttatggcagg
     4081 ttccaaagcg tgatgtacga gcctgacacg ctggtggtga cttatgaata tcgccgcgga
     4141 gaaaacaaaa agggccgctc ggccatgaag ggcgaactga aaaaccgcga agtacggcag
     4201 gagcaggggt atggcgactg tattgactgc ggcatttgcg ttcaggtgtg tccggtcggc
     4261 atcgatattc gtgacggatt gcaataccgc tgtatatcct gcggcctgtg catcgatggc
     4321 tgcaatgaag tcatgggatc tgttggcttc ccgaaagggt tgatccggta tgattcggaa
     4381 gtcaacatga aacgtgacaa ccccgagaaa ccgcatctgt catggaagcg actcaaggtg
     4441 ttgggttatc tggtcgcgtt gacgtcaatg accagcctgc tcgtggtcag cgtcatgcac
     4501 cacacagact tcgagtacag tgtgcaacag gtgaggcagc cattgtttgt ggtgctgtcc
     4561 agcggggcaa tccgaaaccg ctatgacatt cacctgacca accagtcgga tgaggaccag
     4621 gtatttcaca tcagtaccga ggatctgccg ccgggggcgc tggatctcgg gcccatgcag
     4681 aacgtcctgg tgcatccggg caacagcgtg attagcgaag ccagcgtcat actgcagccc
     4741 aatgaggtca agcgctactc gaaattcgag tttgtcatac agtcgctgac cgaagaaagc
     4801 aatcatgccg aggtttcggc gagttttaac agtgagaaat cgactttatg agtttgttga
     4861 caaccttgtt tggcggcctc gtgatggtgg ttgggctgca cctcggactg ggccgggctg
     4921 gcgtgaccaa ctactggcgt ggcgttgtgg ccggggcagt gcccagtttc gtgttattgg
     4981 gatacagcct gttccacaat atgacactgg atgtgatctc gattcacctt gccgtgtttt
     5041 tatcgacggc cacggtgatg accatgattg ccggcgtgaa gcgagaagaa tcatccagcg
     5101 gcatccactg gtcgatcaag accatgatcg tgttttttct ggtgctgttc ctggttgatg
     5161 gcgcgtttgt cagcatatcg accaacggcg tgccgccgga gatcgccgca tggttcttgc
     5221 ccaatgcaag caaaaagccg gtatataccg gattctccgg attgacccgg catgacgagg
     5281 atgcggccaa tgcggtgaat gagcagctca aggaagtcga caagttgcgc aaactgggct
     5341 ggaagatcga aatcgacggt tcaaaccatc tggcgattgg tggtaatggt aaccccttac
     5401 gcgtggtact gctcgatgcg cagcatgtcc ccatgaatgg cgctatcgtg accgagcagc
     5461 tgctacgcgc cgaaacccag cagccgatgc cgaaggtgac gctcgcacag cgggcggatg
     5521 gcgagtacac gggggatctg cctgtgccgc agggtgggaa ctgggtgttg cgcaccgaga
     5581 tcacgtacga aggccagcat atcaagatcg accgtgacat ccgcatcgat attccagagt
     5641 gaaagccgcg gacgtaaaca gctctgaccg cagcggcgag cgttgctgaa catggcggaa
     5701 accgggcagg gaagctgttt ccattgcggg ttgccggtgc cgcccgacag cacgttcaag
     5761 gtgcgggtgg aacaggtcga acaggccatg tgctgtcgcg gctgccaggc cgtagcgcag
     5821 gcaattctgg acaatggtct ggccgagtac tatcgcaacc gcacggccat gccaggcagc
     5881 ggccaggagg tgctgccgga agatgtgcgc aaactggccc tgtatgacca tccggaaatc
     5941 cagcgcagtt tcgtaatcga tgccggtgag cacgctcgcg aggcggtact gatcctggaa
     6001 ggcattacct gtgccgcctg tatctggctc aacgaacgtc acttgcggca gttggctggc
     6061 gtgatcgacg tcgatgtcaa ctatgcaagc catcgcgcgc gcgtgcgctg ggatgatcgc
     6121 aagatccagc tgtcgcgcat tctgcaggaa atccagctgc tcggctataa cgcacatccg
     6181 tacaacgcac gccaggccga cgagttgcgc aagaaacggc gcaagaagga tttgcagcgc
     6241 atcgccatag cgggcattgc cgctgcccag gtgatgatgc tggcggtggc gctgtatgcc
     6301 ggcagctggt acggcatcga cgctcgtctg cgcctgatgc tgcgatggtt cagtctgctg
     6361 ttgaccattc cggtaaccgg tttcgccgcg attccgttct atcgcgccgc gtggagcggc
     6421 attcgcagcg gtcacctgaa catggatgtc cccgtggcgc tggccatcat tggcgccttc
     6481 gtcggtagcg tatgggtcac agtgtttggt ggagcgcagg tgtactacga cacgatcgcc
     6541 atgttcacct tgttcctgct tgcaacgcgc atgctggaaa ccggcgttca ggaaaaatcc
     6601 atcgagtcgg cagaaaatct gctcaagatg caaccggcca tggcgctgcg tctgcggcag
     6661 gggcggcagg agtacgtgcc agtgctggaa ctggaacagg gcgatctcat cctgtccaag
     6721 gcaggcgact cgattgcggc agacggcgag gtcgttgaag ggatttcggg cgtgaatgaa
     6781 tcgctgctca ccggcgaatc gcgctcgata ccaaaacgga ctggtgataa ggtgatcgcc
     6841 gggagcacca atctcgaaag tccgctgacc atccgtgtca ccggtgtggg tgaacacacc
     6901 gtgctcgccg gtatcgtgcg gctggtggac aaggcgcagg cggaaaaacc gcacatcgcc
     6961 caactggcag accgggttgc aagctggttt acgctggcgc tgctggtgtt tactgcgctg
     7021 gtgctgctgg cgtggctgtt gcacgccccg gggaaggcgt ttgaagtggt gctgtcggtg
     7081 ttggtggtga cttgcccatg cgcgttgtca ctggcggtgc cggcagccct cgcagcagcc
     7141 agttcgcatc tgatccgacg tggtattctc gtgctgcgcg gccatgcatt ggaatcgctg
     7201 gcctacgtaa cacacgtggt gttcgacaag accggaacgc tgacgctggg gcagccacgc
     7261 attgcagcga ttcgtccgct ggccgcgctc gacgaagtgc agtgtctgcg tctggcggcc
     7321 agtctcgaac gtgcgtcggg acacccgctt gcgcatgcgt tcgcactgcg agcggaaggg
     7381 ctggtgatac cggaagctgt cgacacacat aacgtcgcgg gatgcggcat gatcggtcag
     7441 gtggaaggtg aaatgatccg gctgggcaat cgcgactggc tcgatatccg catgatggat
     7501 ctggaggcgg agctgcaaca ggagtttccc ggcgcgaccc tggtgtggat ggccaacgcg
     7561 cgcgaagcga tggcggtatt cgtactgcac gacccgctac gcgacgatgc ggcggcggca
     7621 gttgctgcgt tgcgtcagcg gggtgtgcag gtcagtatgc tgtctgggga cgatgcagca
     7681 gcggtgggct atgtggctgc ccagctcggt atcagccagt accgggcacg catgacgccc
     7741 gcagacaagc tggctgccct gcaaaccatg cagcgcgacg gtggcgtggt tgccatggtt
     7801 ggcgatggcg tgaacgatgc gccggttctg gcaggtgccc aggtatcgtt tgccatggga
     7861 accggagcgg atgtcgcgag tcaaaccagc gatatcgtgc tgctatccag ccgcctgggc
     7921 gatgtggatg ttgcagttgc ggtcgggcgt gctgcgcacg tggtcatgcg gcagaatttg
     7981 tggtgggccg tgatctataa tctggcggcg ttgccgtttg ccgcgatggg gtatgtcgcg
     8041 ccctgggttg cagcgctcgg aatgtcggtc agttcgctga tcgtggtgct caatgcattg
     8101 aggttacgct gatgtctcca tccacgctgc ttttcctgct ggccatgacg gcgattttcg
     8161 tgctcctact ggtgctgggg attttctggg cgatcaggag cggtcagttt gacgacctgg
     8221 aaggaccggc acaacgcatt ctcatggatg aggacgatcc gctgctgcct ggcaaccagc
     8281 cacagcctgc tgtgaaagat aacccacctc agcttgcagt tggtacgaag cagctggatg
     8341 acaaaacaac ctgaaagtct cgaattgcac gcagcaaact gtgtgataca atgaacccca
     8401 gtggtacaca ttgagcaaca ccggcaagca gccgtataga ccagactata aggagcatcg
     8461 cgatgatcga atacctgttc ggcaagacaa atttgccgat taccattgtt gtggccttga
     8521 tcgtgtgggc ctggatcagc tttttctccg tggtgttctt ctcgtaatat caccgcagcg
     8581 tgcggtagcg caccgcagca gtgaaaaaat aaaaccccgc cattgcgggg ttttgcatgt
     8641 gcgtcacgaa acacgaaacc ggctcagtac cgaagcgcca gcgctgcagc cgttgtgacc
     8701 ggctcagtgc aactcgggaa gttcgctgcg ctcgagcatc aggtgctgac gcattttctg
     8761 catcgccttc tgctcgatct ggcgcacacg ttcagccgaa atgctgaatt cagcagccag
     8821 ttcgtgcaac gtggcaggat gctcgtcact gagccagcgc gactcaacga tacgccggct
     8881 gcgttcatca agactatgca gcgcctgacc caattcagtg ccggacaagt gctcgtattc
     8941 ttcacgagcc agtacgcgcg tcggttctgc ttcgggatcg gacagccacg ccagtggcga
     9001 aaagctttcc tcgccatctt cgactaccgg ctcaagcgag acatcctggc cactgaagcg
     9061 ggtttccatt tccagcacct cttcgcgccg tacgcccagc gtctgtgcga cctgatctgc
     9121 ttcggcaccc gtaaggctgt ggttgttgtt ctgcttgaga ctgcgcagat tgaaaaacag
     9181 tttgcgttgt cccttggtgg tggcaatttt taccatgcgc cagttgcgca ggatgtactc
     9241 gtgaatctcg gccttgatcc agtgcatggc aaacgaaacc agccggacac cacggtcagg
     9301 gtcgaaacgt ttgaccgcct tcatcaggcc gatgttgcct tcctggatca gatccgcctg
     9361 cggcaggccg tagccaatgt atttgcgtgc aatgctgacc acgacgcgca gatgggtcat
     9421 gaccagcttg cgtgcggcct cgacatcctg gtaatcgcgc agacgcactg cgagcgacgt
     9481 ctcctcctcc tgtgacagga tcgggtaacc gttgacagtg tgaatatagg cctccaggct
     9541 cgtggcctgg ttgctcatca tgggaaaatt catcgcggtg acagtcattt cgggacaatc
     9601 ctcctgtaac caattttagc actccttgtg agagagtgct aacaggaaaa agttcctccg
     9661 gtagtggtca gcgcagcaag gtgtagcgca gtgccagcca tgcgcccagc cagcccagcg
     9721 ccgcggccac ggcggtgagt gtcagcccgg cgctggccgt gggatagccg agtacgaagg
     9781 cgctgccata cagacgggcg agttcgccga gttcaggagt ggcaagccag atggcgatgc
     9841 agacaatgcc ccacgccagc acgcccgaca gcagcccctg cagggcgcca aagtaaagaa
     9901 acggccgctg gatgaatcgg tctgtggcgc caatcagctg gctgacttcg atttcgggca
     9961 ggcgggtaag gatctgcagg cggatgatgt tgccggaaat ggtcaccagg cctgcagcaa
    10021 gcaacatggc cagaaaggtc gtcagacggt cgccgagcgc caccagtgcg ttcagtcggg
    10081 acacccagac ctggtcgccc agaaccagat cgacgccagc caggtgcgac aggcgttcgc
    10141 tccactcgcg gttgagcgcc ggatcgatgc gcgtcggtgt gagttgccag gcgtcgggga
    10201 gcggattgtc gggcaattct gcgctcaggt cgggcagacc gagatgctgt gacagttcat
    10261 gcaatgcatc gttgcgcgaa atgaaacgcg ccgatgccag accgggtagc gcatgcacgc
    10321 ggcggtcgag cgctgcccga tcgtcgctgg acgcgtcggt gcgaatgtag acggtaagcg
    10381 tgccttgcgc cgggaggcgg tcggccaact gctgcaggct ggccagtccg gcggccagac
    10441 ccgccggcag gctgagggtg atacccagac tcaccagggt aaaaaaagtg gcaaacggtg
    10501 cagttgccag tcggtgaagc gaagatacga acgcatggcg atggtgggcg agccagattt
    10561 tcacgatgca aggctcccat ggtcgaggcg tacgatgcgt tgccggccgg ccggaactgc
    10621 cgatggatca tggctggaga tgatgacagt ggtgcctgtg cggtggaatt cgctaaagat
    10681 gcgcatgatg tcggctgtgt agccgctgtc caggttgccg gtcggttcgt cggccagcaa
    10741 tagtggcggt cgtgaaacaa tggcgcgcgc gatgcacaac cgctgctgtt cgccgccgga
    10801 aagggcaaca ggcatggatt tctcccggtc cagcaggcca accttgtcaa gggcggcgcg
    10861 tgcgcgggaa aaggcgtcat ggcggcgaaa gccggcgatg tcgagtggca gcagcacatt
    10921 gtggatcacg ttacggtcga acagcagctt gtgatcctgg aacaccagtc cgatgttgcg
    10981 tcgcaggaag ggtagcgccg aacgacggat gcgactgata ttctgttcgt tgacccagat
    11041 gttgccgctg gtcgggcgtt cgatcgcgga aatcagcttg agcagcgtgg atttgccggc
    11101 gccggaatga ccggtaagaa acaccagttc gccggattcg atgtgcagcg taacctcgtg
    11161 cagcgcgtca tgaccacccg ggtagcgttt actgacctgt tcaaagcgga tcatggcgcg
    11221 gactcgtcaa acagggcatc gatgaattca tgggccgaaa acggacgcag atcgtccatc
    11281 tgctcgccga caccgatgaa acgcaccggc ttgggccgtg tgcgtgcgat ggcggcaaca
    11341 atgccgccgc gtgccgtgcc gtcaagcttg gtcagcacca gtccggtgac gccgagcgca
    11401 tcgtcgaacg ccttgacctg cgccagcgca ttctgaccgt tgtttgcatc gagcacgagc
    11461 agcacctcat gcggggcctg cggatcgatc ttctggatga cgcgcttgat cttgcggatt
    11521 tcatccatca ggtgcagttg ggtcggcagt cgcccggcgg tgtcggcgag caggatgtcg
    11581 atctggcgcg cgcgggcggc agagaatgcg tcgtggatca cggcagcggg gtcggcatcc
    11641 tgctgggaaa tcaccgcaac cgagttgcgt tctccccagg cggagagctg ctcgcgagcg
    11701 gcggcgcgga atgtgtcgcc ggcagcgagc agtaccgact tgccctgttg ctggtagaat
    11761 ttcgccagct tgccaatggt ggtggtcttg ccggcaccgt tgacacccgc cagcatgatg
    11821 acgaacggtg aatgcgtgtc ggtgtgcaac ggctgttcga gcggtgtgag cagttccagc
    11881 atcgcctcgc gcagcgcgga acgcagttgt gcggcatccg acaggctgtc gcgccgcacc
    11941 tgctgacgca agcgttcgag caggaagccg gtggcttcca cgccgacatc tgcggtgagc
    12001 aggatggctt cgagctcttc gtacagcgca tcgtcaatgg cggcgccagg cgaaaacagg
    12061 ccaccgagct gacgaccaag ctgctcgcgc gtgcggcgca agccctgacg cagacgggct
    12121 gcccagccgg cgggacgggg atcagcggat tcctgcaacg aagaagaatc cgcggagggc
    12181 gattttttga agaaaccaaa cacggccggg gtaatccaag cgatagtgaa cgacagggca
    12241 ggcgcgatta ttctatcacg cgtcatggtg aagtggacga aacgggagca ggggtatgaa
    12301 gaggtggtgg gcaggattgg tgtgcatgct gagcgtgacg ctggcgcatg ccggagtgca
    12361 gcagtttacg ctggacaatg gcttgcgggt gatcgtgcag gaagatcacc gttctccggt
    12421 ggtggtgtcg caattgtggt accgggtggg cagcatggac gagttcaacg gatcgaccgg
    12481 tctgtcgcac atgctggaac acatgatgtt caaaggcacg aaaactgtgc cggacggtca
    12541 gttttcccgg ctgatcgcat cggtcggcgg tcgggagaat gcgttcacca atctcgacta
    12601 caccgtgtac ttcgagcagt tgcaaaagga tcggctcggg ctggcgtttc gcctcgaagc
    12661 cgaccggatg cacaacctgt tgctcgccga ggacagtttc aggcaggaaa accgtgtcgt
    12721 gcaggaagaa cgccgcatgc gcaccgaaga tcagccggaa gcgctggtgt acgaaaacct
    12781 gatggccacg acgttcgaag ccaaccccta tcgcaggccc gtcatcggct ggatggacga
    12841 catccgtaac ctgactgtgg acgatgtgcg tcactggtac cagagctggt atgcgcccaa
    12901 taatgccacg ctggtcgtgg tgggtgatgt gcagcccgac gcggtacgcg cactggcgca
    12961 gcagtatttt ggcggcatcg cctctcatcc gctgccggtt cggaaaccgc aggaggagcc
    13021 gcagcaggtg ggtaccaagc gcatcgtggt caaggctccg gccaaggtgc cctatatgat
    13081 gatggcctgg cacgtgcccg tgttgcgcga ttatgcgcac gactggcagc cgtacgcgct
    13141 gcaggttctg gccggcgtgc tcgatggtaa ttcgggggcg cgactgggcc gggtggtcgt
    13201 gcgcgaacag cgcatcgcca gtgaggccag tgcgagctac gatctggtcg gtcgcgggcc
    13261 ggggctgttt gtgatcgacg ccacgcccat gcagggcaag accatggccg aactggaagc
    13321 cgcgctgcag gcacaggtac gttcgattgc cgaacagggg gtgtcggccg atgaactgcg
    13381 acgtgtcaag tcacaggtga tcgcggacaa tgtctaccgg cgcgattcga tgttctatca
    13441 ggccatgctg ctgggcgaat acgtgaccga cgggttgccg gtggaggcag tcgccgccca
    13501 ggccgatcgc gtctcggcgg tgacggcgca acaggtgcag caagtggcga agcaatatct
    13561 ggtcgacgat gcgctgacag tggccacgct cgatccacaa ccgtgggacg agcaccacga
    13621 gcgcccggct gtcgatctgc atgatttgag gtgaatgaga tgatgcgtac aggaatgata
    13681 ttgggcttgc tgttgtggag cggtgcggcg gttgcggcat tgccgattca gcactggcaa
    13741 ctcgacaatg gtgcccaggt gtattttgtc gagaatcacg atctgccgat tatcgacgtc
    13801 agcgtcgatt ttccggcagg cagtgcgtat gatccggtcg gcaaggaagg ggtggccagc
    13861 ctgacccgac ggttgctcga tcagggggcg ggggggctga ccgatgtgca gatcgcgggg
    13921 cagcttgctg acgttggcgc aacgctgggc gggcatttcg atgccgaccg cgccggggta
    13981 acgctgcgta cgttgagccg gccggatgaa gcgcaccagg ccttgtccct gatgacggcg
    14041 gtgatcgata agcctgcgtt tgccgcttcg gtggtggaac gtgaaaaggc tcgcgtcatc
    14101 gatgcgttgc gtcagtccga tggcgagccg caagtcattg ccgcagtggc attccagaaa
    14161 gccgtgttcg ccgggcatcc ttatgcgcat cgtgaaacgg gcgagattgc caccgtgagc
    14221 ccgcttgcat ccacggatct gcaagcgttc tatcgctcgc attacacagc gggtaatgcg
    14281 gtgatttctc tggtggggga tatgacgcgt gctcaggccg atgcaattgc gcatcagctg
    14341 acggcgggtc tggtgccagg caagggtgat gtgccgccgc tgccatccgt gccggcgttg
    14401 acggacgccc aggtgcggaa tattccgttc ccgtcgcacc agagccagat cttcatgggt
    14461 cagccgggtg tggcgcgcgg tgacccggat tactatgcgc tctatgtcgg caactacgtg
    14521 ctcggtggtg gtggtttcga ttcgcgtctg atgaacgatg tgcggcagaa acgcgggctg
    14581 gcctacagtg tgtacagtta tttcattccg atgcgggtag gcggcgtgtt ccagatcggc
    14641 ttgcagacac gtgccgacca gaccggtcag gcgctgcagg tggtgcggca gacggttgat
    14701 gacttcatgg cccagggcgt gaccccggcg gaactgacgc aggcgaaaaa cagcatcgtc
    14761 ggcggtttcc cattgcgtat cgacagtaac gccaaattgc tcgactatgt ggcgatgatc
    14821 gggttttacg ggttgccgct ggattatctc gataccttcc cgacacaggt gcagaccgtg
    14881 actgcaaacc agattgccga agcgttccgg cgtcatctga atgcgggaaa aatggtgacg
    14941 gtcgtggtcg gcgcggccac ggcggcaaaa tgaatggtgc gcacggatga tgcggtcgcg
    15001 gtgcgcgtga tggccgggca aggattggtc aagggctcgg tacgcattac cggcggcaag
    15061 tggcggcggc gcgtgctgca ttttgcatcc cgtgccgcac tgcgtccgac ggcagacatg
    15121 gtgcgcgaac gtctgttcaa ctggcttggg caagacctga ccggctggca ttgcctcgat
    15181 ctgtttgcgg gcagtggggc gcttggtttc gatgcagctt cgcgtggtgc ggactgcgtg
    15241 tgcatggtcg aacgggacgc cgcagcctgc actgcgctgc gacagaatgg cgacctgctt
    15301 ggtgcgtcgg ggcaggttac agtatattgc gatgatgtgt tcggctggct gcagcgttca
    15361 cctcaggcgg tgggatgtgc gccgtttgat ctggttacgc tcgatccgcc gtttgcccag
    15421 ggtgggcagg aacgcgtgct cgacgtgttg tcgcgtggct ggttgcggcc gcaggcacgg
    15481 gtgtacgtgg aacacgatgg ctcgctcatc gttccggaag gatgggatgt gctgcgcgcc
    15541 gggcgtaccg ggcaggcgca ttattgtgtg ttgagctggg gggaagcatg atccgaacgg
    15601 cggtctatcc ggggacgttt gatccggtca cgcgcgggca cgaagacctg gtgcggcgtg
    15661 cgctgcgcat tgcggatgag gtcgtggtcg ctgtcgcgct gggcggacac aagcggccgt
    15721 tttttgacct ggatgagcgt gtggagatgg cccgggaagt gtttgccggc atggctggtg
    15781 tccgcgtcac cggtttttcc gggctgctga tggattttgt tgctgatcag ggagcgcacc
    15841 tggtgctgcg tggcctgcgt tcggtgtcgg atttcgagta tgaattccag cttgccggta
    15901 tgaaccgcaa cctcaatccg cacatcgaaa ccgtgtttat gacgcctgcc gaacagtaca
    15961 tgttcatttc tgccagcatg gtgcgtgaaa tcgcccagtt gggtggcaac atcgatccgt
    16021 tcgtgcatcc gctggtggcg cagcgcctgc gcgacaggat cggcgcgtcg ggcgccatgc
    16081 agtgacgcat ggggcgcgct ctgttagaat gcgcttattg agcgtattgt cgccggaaac
    16141 tgttttgcct gaggaaaaaa gacatgtccc tgatgatcac cgatgaatgt atcaactgcg
    16201 acgtttgcga gcctgaatgt ccgaacgagg ccatttcgca gggtgagacc atttaccaga
    16261 ttgacccgaa caaatgtacc gagtgtatcg gtcattatga tacgccgcag tgcgtggaag
    16321 tgtgtccggt cgattgcatc atcaccggtg tggaagaaac ccaggagcag ttgaacgacc
    16381 gttatctgca actgaccggt caacactgaa tcgtcagttc agtgccgacg cctggccgtg
    16441 cagttgctgc tgcagccagg tgcgtaacgc tccgatttcc tcggcgcaga tttcgtgccg
    16501 tatcggccag aggtgcggcg tgatgccgag ttgcggttgt gcagcgtagc tggcctgact
    16561 ccatgccagg ggtaccaccg ggtcttcgct gccatgcgcg gcccagactg gcaccgccgg
    16621 tgctgacggc accaggtgcg gtagccagcc cgacagggcg gcgatgcccg ccagcggcag
    16681 gccgagcgtg aggccgctat acagggccag cgcggcgccc tgagagaatc cggccagcgc
    16741 gatgcgctgc acggccacgc cgcgctctgt ctcacggcgg atccagtgcg acacctgttc
    16801 gcgcgctgcc cgcaatcctg catcatcctg ctgcatgtcg ccgtcggtgt gcgtcaggtc
    16861 gaaccacgcg cgcatcggat atccgtcata catcgaaatt ggtcgtaccg gcgcgtcagg
    16921 cagtacgaaa cgtacatgct cgatttccag cgcctgggcg atgggcagca gatcgtggcc
    16981 atcggcaccc agtccgtgca gcagcaacac gctggcatcg ggccggccgg cgctccattc
    17041 cagtgggagg gtgtcgtcgt tcatggcagt tatccttgta gcagttggct ttcggcgctg
    17101 gccaggttgt ccgggttacc gcgtagcacc agcacgtcgc ccggcaggat gcgcatttcc
    17161 ggttgcgggt cgagaccccg gatgttgcgc cggcgcacca ccagcacttc cacgtcgaga
    17221 tccgtcagtc cggtttcggc gatggtgcga tgggcagcgg ccgactgctc ggtaatcagt
    17281 accgacgcca gccgtggctg ggcggcatcg ctgatggccg aatcggcgtc gctcacgccg
    17341 cggaaaaagc cgcgcagcag actataacgc tgttcgcgca cgctgcgcag ttttttcagc
    17401 acacggctaa ggggtacgcc gagcagcagc agcgcatgtg atgcgagcat cagcgagccc
    17461 tccatgacct ccggtactac cgccatcgcc ccggcggcca tcaggctgtc gagatcggtc
    17521 tcgtcgcgcg tgcgcacgat caccggcagg tcagggcgga tttcctgtac cagttgcagg
    17581 attttcatcg acgcggcgtg gtcggaaaaa gtgatgacca tgccatgtgc gcggttgagg
    17641 cctgccgcct tgagcacctc gcggcgcgac gcgtcgccaa acaccaccgt ttctccagcg
    17701 gccgaagcat cgtgtactcg tgccggatcg gcgtcgagcg cgatgtagct gatctgttcc
    17761 gattcgagca gacgggcgag actctggccg ctgcgaccgt aaccgcagat gatgaggtgg
    17821 tcgttgatac ccatgctgcg cgtcgccacg tcgtgcagtt cgcgggcgcg gtgcgcccat
    17881 tcgctgttgc atgcccaacg cacgatacgg tccgactgct gcaggatgag tggcgcagtc
    17941 atcatcgaca gcaccatcgc tgccaggacg ccttgtagtg tattgccgtg taccaggccc
    18001 aatccggcgg cctgcgacaa cagcacgaag ccgaattctc ctgcctgcgc cagccccagt
    18061 gccgtacgca gcgcaaccgg cgcatcgttg ccgaacaggc ggccgatcag ccagaccaga
    18121 ccggttttgc tgaggatcag gctcaacagc aagatgatga cccatcccag gtgttgccag
    18181 acattgagcc agtcgagttg catgccgagg gtgacgaaaa acaggcccag cagcacatcg
    18241 cggaacggtt tgatgtcgtc tgccacctgg tagcggaatt cggtttcgga gatcagcatg
    18301 ccagccagaa aggcgcccaa tgcaagcgac agtccggcac gctgggtgaa ccaggccagg
    18361 ctgagcgtga tcagcagcac attgagcatg aacagttcgg acgatttgtg actggcaacc
    18421 cagttgaacc atgggcgcat gagcttgtga cccaggaaca gcagggcaga cagtgcgatg
    18481 ccggccttga ttacggccag cagcagcggt tgccagagcg catggctgcc actggccagc
    18541 gcaggcgcaa ggatcagcag cgggatcacc gccagatcct gaaacagcag cacagcgatg
    18601 atctgatgcc catggttcga ttgcagcgcc aggcgctcgg cgagcatttt gctgacaatt
    18661 gctgtcgagg acatggccaa tgcaccgcca agcgcgatac cggcattcca gccgagaccg
    18721 gccagatgtg cggtcaccgc agcaagcagg atgaagctca gcacttgtgc tccgcccagg
    18781 ccaagtgcgg tgttgcggat tgccttgagg cgcggcaggc tgaattcgag gccgatgctg
    18841 aacatgagga ataccacgcc gaattcggcc agcgccgacg tgtccgggct gtccgggatc
    18901 cagtcgaggc tgtgcggccc caggatgatg cccaccagca ggaaacccag gatggaaggc
    18961 aggtggaggc ggcgaaacag cgcggacacg accacggccg cggccagcaa cagaacgatg
    19021 agcgcaagag tcgaatgcat tcggcaaggg tagaggcatg caccgggcaa tgcaagcgct
    19081 gcggccgtgg cggtgcggat gtgtccgggt tctgctgggg cagcggatag ggtaaaatgc
    19141 cagttcagtc aacgatgatg gatttatgat cgctccttct ccttccagcg aaatcgaccc
    19201 acaagccgtg ctggcgcgtg cccgggatgt gctgaacatc gagtccgaag ccattctggc
    19261 cgtgcgtgac cggatcgacg aacagttcgt tcgtgccgtg tcgctgatcc tcgcctgccg
    19321 cggcagggtg gtggtgagcg gtatgggcaa atcgggacat gtgggcaaca agatcgccgc
    19381 tacgcttgcc agtacaggca cgccggcttt ctttgtgcat ccgggcgaag ccagccacgg
    19441 cgacctgggc atgattgctg cggacgatgt atgcatcgcc ctgtccaatt ccggtgagag
    19501 tagcgaactg acggctatcg tgccgctcat caagcggcgt ggggcgcggc tgattgccat
    19561 gacgggcaat ccacgctcca cgctggcgat cgaggcggat gttcacctcg atgccagcgt
    19621 ggcgcgcgag gcctgtccgc tcaaccttgc gcctactgcc agtaccactg cggcgctggc
    19681 gctcggtgac gcactggcca ttgcgctgct cgaggtgcgc ggttttggtt cggaagattt
    19741 tgcctggacc catcccggtg gcgcactggg cagacgattg ctggtgcatg tgcgcgacgt
    19801 gatgcatgca ggcgagcgct tgcccatggt cggtcgcgat gcgctgctgc cggaggcctt
    19861 gcttgaaatg acgcgcaagc ggctgggcat gactgccgtg gtggacgccg cgcagcatgt
    19921 gctgggcatc tttaccgatg gcgacctgcg ccgcgttctg gaaaagtcgc ttgatgtccg
    19981 ccaggtcaag gtcctggacg tgatgacgcc gcacccgcac tgcatcgacg ccgaacagct
    20041 ggctgtggtc gctgtggaac gcatgcagcg ccacaacatc aacggactgc tggtggtgga
    20101 tgcgcagggg cggcttaccg gtgcgctcaa catgctggac ctgctgcgcg caggcgtggt
    20161 gtaacaggaa aacggacgga acatgaaaca ggcaaccctg agggcccagc cggtgcggct
    20221 ggcaatattt gatgtcgacg gcgtcatgac cgatggccgt ctgtattttg gcgatgacgg
    20281 gcaggaatac aaggcgtttc attcgcttga cggacacggc atgaagatgc tggcgcaaag
    20341 cggcgtgcaa ctggccatca ttaccggacg aacttcgcag gttgtggtgc atcgcgcacg
    20401 caatctgggc atcgagctgg tctaccaggg cgcgggtgac aaacttgcag cctatgatga
    20461 gctgctgcgg cgtgtcgggc tcacgcctga acagacggcc tacatgggcg atgacgtggt
    20521 cgatttgccg gtgttgctgc gctgcggact ggcgctgacc gtgccgacgg cgccgggcga
    20581 ggtgcagcag cacgcgcatc tggtgaccgc gcgtgaaggg ggtcgcggcg cggtgcgtga
    20641 agtgtgcgaa ttcatcatga aggcgcaggg cacctgggcc gggttgttgg cgcattacct
    20701 gcaatgaccg atgagtaacc ggccgttgta cgtttttttg ctgatgatgc tcttgattct
    20761 ggcggcaggc accgcctggc tcgagtatgc cgtacagaca caccggggcg gcaagtggtt
    20821 gtcaagatca cgcagcccgg attacatgat ccgtcacttc gtcgttacgc gcaccaacct
    20881 gtccggtcag gtggtatatg tgctgcatgc tgattttgcg gagcattttc tcgacgacga
    20941 tacggcgacg ttgacgctgc cgcatctggt ggcgcaagat gagaagcagg ggagcgtgga
    21001 catccgttcg gatcgcgcgt tcgtgaccgc caaggcgaag gaagtgagat ttttcggcca
    21061 cgtggttctg gtgcgtgaca tgcacgacgg ccagggaccg ctgacgctga cgacggatta
    21121 tttgcaggcc tttccggacg accagatcat ccagaccagt catgcggtcg aaatcgtggg
    21181 tgaaaaaatt cacatgaccg cgaaaggtct ggtgatgaac aacgccacca gacagttgca
    21241 tctgttgcat gacgtaaagg aacaatatga accggcgcat tgaacttggc ttgccgctcg
    21301 tggcagcgtg gctgctgatg ctgtggctgg cggggccagc cctggccgag caggcggatc
    21361 gcagcaagcc ggtcaatatc gaggccaaca gcgtgcagat ggacgatctg cacaagacca
    21421 gcctgtatga aggtgatgtg atcctgaccc agggaacgct gcaggtgcat gccgaccggg
    21481 tagcagtcaa acaggatgcc gcgggtttca atcaggccac cgcctgggga aatccggtgt
    21541 attttcgcca gaaacaggac aattccgacg agtacctcga aggctgggcc aaccgcctcg
    21601 acatggacaa tgccaagaat accgtgctca tgacgggcaa tgcgcgtctg aaaaagggtg
    21661 ccgacgagat gcatgccagc tcgatgctgt acaacacggc cacccagcaa ttctcggcgc
    21721 agcgtgatac cagtcttcgg cccgatggcc gacctgaccg ggtgcgtacg gccattttcc
    21781 ccaaggcgac ccaaacggcg cagccggctc ccgatagcgg cgtgcccctg cagacatccg
    21841 gcgcaatcca ggacggaacg gccagccatg agtgagttgc gcgccgaaca cctgaaaaag
    21901 agctatcgcg cgcgcgcggt ggtcaaggac gtgtcgatcg aggtcaggag cggtgaggtc
    21961 gtcggcctgc tcggacccaa tggcgccggc aagaccacct gtttttacct gatcgtcggt
    22021 ctgctcaagc ttgacggtgg tcggatctgc cttgacggca atgacctcgc gaccatgccg
    22081 atccacaagc gtgccgggct cggtctgagc tacctgccgc aagaagcctc cattttccgc
    22141 aagatgaccg tggcgcagaa tatctgcgcc attctcgaac tgaaagatta cgatgacgac
    22201 cagatcgagt agcatctgca aaacctgctg gccgatctga gcatcggaca tctggccgat
    22261 gctccggcca tgagcctgtc cggtggcgag cgccgtcggg tggaaatcgc acgcgcgctg
    22321 gcaatgaatc ctcgattcat cctgctggac gaaccgttcg ccggcatcga tccgatcgcg
    22381 gtgatggaca tccagaagct gatccgtttt ctggtggagc gcgatatcgg cgtgctgatc
    22441 accgaccata atgtacgcga gacccttgga atttgcgatc gagcctatat tatcaacgat
    22501 ggaaccgtgc ttgccgccgg ccggccggac gacatcgtgc gcaacgacaa tgtgcgcaga
    22561 gtgtatctgg gcgaacattt ccgtttgtga accgatcatg aaacaaggac tgcagctcaa
    22621 attctcccag cacctgacac tgacgccgca actgcagcag tcgatccggt tgctgcagct
    22681 gtccacgctg gagctgaacc aggaagtcga gctcatgatg cagaacaacc cgctgctgga
    22741 acgcgaggaa ccggccgagg aaggcgtggc ggtgcgtagc ggcgagtacg aaggggaggt
    22801 tgtcgtcaag gaagccccgg agccggcgga gtcggcgatg gccgaagcag gcgagctgga
    22861 atggagcgat gcttcgtctt atggcacgcc ggatgaggac gatgcattcg aactgcaggc
    22921 gcctgcgcag gtcatgagcc tgcgcgagca cctgcaatgg caggccaacc tgagcgcgct
    22981 gtccgagcgc gaccggcggc tggttctgct gctgatcgat gcgctggacg aacgcggcta
    23041 cctgacccag acgctggaag aaattttcga gatgttgccc gaagaactgg gcgtcgggct
    23101 cgacgaactg cagatcggcc tcaagtatct gcagcatctc gatcctcccg gtgtgggcgc
    23161 gcgcgatctt ggcgagtgtc tcgctttgca gctggccgga aaatccgaag acacgccggg
    23221 tctggcgctg gcgaaacagg tggtcgaacg cgaactcgcc gcgctgggaa cgcatgatta
    23281 tgccgggata aaacggcggc tgcgctgcag cgacgaggcg ttgcgcgaag cgcgtgagct
    23341 ggtgacccag ctcaatccgc ggcctggcga ggcatttggc gccagcgata cgcgttacat
    23401 cattcccgat gtgatcgtcg acaaggtcaa gggcacatgg gtggcgcggc tcaatccaga
    23461 agccatgccg cgcctgcgcg tcaaccagat gtacgctgac atcctgtcgc gcaaccgtga
    23521 taccgggcat cagcagctgg ccggacagct gcaggaggcc aaatggatga tcaagaacat
    23581 catgcagcgt ttcgatacca tcctgcgggt gacgcagtgc atcgttgaac gccagcgcca
    23641 tttcttcgaa catggcgaag tggccatgcg cccattggtg ctgcgtgaag tcgcggaggc
    23701 ggtcgaattg cacgagtcga cggtttcgcg tgtgaccaca cagaagttca tgatgacacc
    23761 tcgcggcatt ttcgaactca agtatttttt tggcagccat gtcagcaccg aagccggcgg
    23821 cgcgtgttca gccaccgcca ttcgcgcact gatccggcaa ctggtcgggg ccgaggatgc
    23881 gaaaaagccc ctctccgacg gacagatcgc cgaactgctt gggcaacagg gcatcgtcgt
    23941 ggcccggcgt accattgcaa aataccggga atcgctgcag attcctccag ccaaccaacg
    24001 caaggcattg tgagcatgcg acccgctccg ggacatgtgc tgcgcggcgt agcgggctgg
    24061 aactgttcat ggcgtcgcct cgtgacggga accggctcgc agtccgatgt ttccacgtgc
    24121 ggcgacgcgg cgtggcgcgg cgggaacgcg cgttgcgtct ttatcggcaa ggagccggcc
    24181 gggcctgatg tttccggtgc gcccggtctg ctcccatcgt tcccgtaacc cgtgaaaagg
    24241 caagtatcat gaatctcacc attaccggcc gccaccttga aatcaccccc gccatccatg
    24301 attacgtgct ctcccggctt gagcgcatca agcgccatgc cgacaacctg atcgatgtga
    24361 gtgttgtgct gtccgtggcc aagctgatcc acagcgtcga agccaacgtg catctgagcg
    24421 gcaaggatat cttcgtgcag gcggaagacg ccgacatgta tgccgcgatt gataatctgg
    24481 ccgacaagct ggaccggcag atcgtcaagc acaaggagcg caacggcgat gtgaatcagg
    24541 ccagcggtgg gatcaagcac cagtcggcag gtgaggaagc ctgacgcgat gaaccggatc
    24601 gccgcgctgc tcgatgttca caggattgca ctggatgtgc gcagcgacag caaggcggat
    24661 ctgttttcct ggttgggaca acggttcgcc ggcgatctgg ggcttgcgcc ccaggtcgtg
    24721 agtgccagcc tgcgggcgcg ggaggatctg ggatccacgg cgctgggtca gggcatggcc
    24781 attccgcatg gccgcctcaa gggattgaaa acggccgcgg gcgtactggt acgcttgcag
    24841 acgggactcg agttttccgc tccggataca gtgccggtgc ggttgctgtt cgtgctgttc
    24901 gtgcctgttc atgcgaccga tgagcatctg cagattctgg gcgaactggc acagctgttt
    24961 ggtgaccgtg acatgcgtga gcgtctgctc ggttgtcgtg atgaacatga ggttctggaa
    25021 tgtatccggg actggcgcgc ggagtgaaca tgagtctgtc catttctgtt cagcagatgt
    25081 tcgatgcgct caaggaggcg ttgtcgctgg tctgggtgtc tggccaggcc ggcagtgaaa
    25141 atcgcctgat gagcgatacg gtgcagaagc catcgctggc gctggttggc cacctgaatt
    25201 tcgtgcatcc gaaccgggtg caggtgcttg gttgcgccga aatggattac ctggcaagac
    25261 tcgatgccgg tggtctggcc caggcggtcg gcaacctgtt ttccactgag ctggccgcag
    25321 tgatcgtcgc caatggcgag ccggtacccg agccattgct ggcagcctcg caccgcaccg
    25381 gtacaccgtt gttcgcgtcg ccgcagccca gccccgagct gatgtcggtg ctgtcgcatt
    25441 acctgtccac tgcgctggcc gaaaccacca gcgtgcatgg cgtgatgctg gaggtgaatg
    25501 gcatgggtgt catgctcgca ggtgagccgg cttccggcaa gagcgagctg gcgctggaac
    25561 tgctcacgcg cggccatcgg ctggtcgccg acgatgtggt cgatctgcac aaggttgcac
    25621 cggacacact gagcgcgcaa tgcccgtcat tgctgcagga tttcctggaa gtgcgcgggc
    25681 tgggcattct tgatattcgt gcgctgttcg gtgagctggc catcaagctg aagaaaaatc
    25741 tcaaactgat cgtgcatctc aagccttacg acgaactggc cgaacgcaac caggaacgac
    25801 tcgacatgca ggagagtacg gaggtcatcc ttggcgtacc cattccgaag gtgtacattc
    25861 cggttgcggc tggccgcaat ctggccgtac tggtcgaggt ggcgacgcgt aaccacatgc
    25921 tgcgcaagcg cggcgtgaac agtgtggaaa atttcattgc tcgtcagcgc gagcacattc
    25981 agagcggaga atgacgtgtc cgacacggac agcctgcaac tggtgctggt gagcggcctg
    26041 tcggggtcgg gaaagagtat cgcgttacgc gtgctcgaag acgccagtta ttattgcgtc
    26101 gacaatctgc cggccaccat gctggaagat acgcttcgcg tactggccgg ttatggcgaa
    26161 tttcgcgtgg ccatcagcat cgatgcccgc agtcgcgatt cttggcggca tctgcctgat
    26221 ctgcttgaac ggttgcgtgg acaggtgaac gtgcggctgc tgtttctcga agccaagaca
    26281 gacacgctgg tgcgacgcta ttccgagacg cgacgtcgcc atccgctggc caatgccgac
    26341 cgcgccctgc gcgaagcgat cgagtgggag cgcgaactgc tggaaccgat cgcgcgcatt
    26401 gcccatcgta tggataccag cgacttgtcg gccagcacgt tacagcactg gatcaaggac
    26461 ttcgtggcgc agaaagacgc gcgcctgacg ctgctgttta cctcgttcgg tttcaagcac
    26521 ggcattccgc tcgacgctga catggtgttc gatgtgcgtt gtctgcccaa tccccattat
    26581 gatccgcgac tgcgtccgct gaccgggcgt gacgcgccgg ttatcgagtt tctgcaacag
    26641 aacggacagg tggcggaaat gctggccgac attcgcaacc atgtgaccaa atggctgccg
    26701 tgttttgtgc gcgatcaccg tgcctacctg acggtggcca ttggctgcac cggcgggcag
    26761 caccgttcgg tgtacctgtg tgagcaactg gcccagcatt tttcccgcga ggaacagacc
    26821 ttgctgcgcc accgggagct gggttatggt tgcgcgcagt gaggtcatga gtggcctgct
    26881 gtatggtgtc cgttccggtg actggttcac ggcagggctg ggtgctgtgc tggtattggc
    26941 tctggcctgg cactactggc agccgggagc cggcgagcgg ctgctggtca aacgcggtgg
    27001 aaatgtgttt ctcgaaacat cgctggcacg tgaccgggat gtgaccgtga acggaccgtt
    27061 gggcgttacg cggatcgagg tgcgtaacca tcgcgcccgc gtgatcagcg atccggggcc
    27121 acgtcagctg tgtgtgcgtg agggctggat cagtcgcagc gggcaagcag caatctgtct
    27181 gcccaaccag gtcagtgtgg aagtggttgg caaggcaggc ggctatgaca gcctcagtta
    27241 ctgaagaaat cgaggtcact gcagaagatc accgcctggc acgcctggcg gcgctggccg
    27301 tatcgctgat gtgggtcgat gccgcgctgc cgtcaccatt gccgggagtg aagccgggtc
    27361 tggccaatat tgtcaccctg ctggtgttgc gtcgctacgg ttttgccaca gcggcctggg
    27421 tgagcgggtt gcgcgtggtc gccggcagcc tgctgtttgg cagctttctc acgccgggat
    27481 tcatgctcag cgtgtctggc gccagtgcca gcctgctggt attgtggctg gcgcaggctt
    27541 tgccggagaa atggtttggt gcggtcagcc tgagtgtgct ggcggcgttt tttcatatcg
    27601 gtgctcaatt ggccgtggtg gcgctgtggt ggatgccgtc agtgaacctg atgccatcca
    27661 caccagtgtt tgccgctgcc gcctggatcg gtggggtggc caacgggctg ctggccgagc
    27721 gcctgcttgc aaaggaatac catgcagcca ccgcgtagcg tgacgctggc ctggaccggc
    27781 gcgtcaggta tgccatacgg cgtgcgcctg ctggaacagt tgctggcgtc gggtattcat
    27841 gtctatctgc tggtcagcgc agcggcacag atcgttgccc ggcaggaaat gaactggagt
    27901 ctgccctcgc agccggaacg actgcaggcc gaactcgaag cgcgctggcc gcaggccagt
    27961 ggcacgttac gcgtgctcgg gcgcgacgag tggtttgcgc cggtcgcctc cggttccaac
    28021 ccggccgatg ccatggtggt ctgcccctgt accatgggcg cactggcggc cattgctcag
    28081 ggactgtcgg agaatctcat cgagcgtgct gccgatgtct gcatcaagga acgacgtacg
    28141 ttggtgctgg tgccgcgcga gacgccattt tctgtgctgc atctggaaaa catgctgcgc
    28201 ctggcgcgtc tcggggtggt gatactgccg ccgaatcccg ggttctacca tctgccgcgc
    28261 tcactggacg aaatcgtgga tttcgtcgtg gcgcgcattc ttgaccagtt gcatgtgcct
    28321 catgcgctca tgccccgttg gggggatgcg ggcaccagct agccgacggc ggggtgatgc
    28381 tggagggaat gctgtttacg ggtggaatgc cgttgcgtca tgattccggt tgaattggtc
    28441 gtgcagttgt gtaagatgga ctggcgcagc gtggcacagg ttgcatcact acgcattctg
    28501 gccgatgttg ccgattttat cctgacaagg gataatcaag ggttggtaat aactgtggtg
    28561 tttgggactg actgaattgg atttttccag cattttttct cgcaaggaca aaaagaacac
    28621 cgtcccggtc tcggaacccg ccctggtcaa acccaagggc cgggagctgg cgatcgccca
    28681 gatcaatgag cggccgctga ccgaacagca tttcgatacc gggctgatgc agtcacggat
    28741 tgaagtgacg gaagtgtcct cgcactttcc gcccggaatg gaagacgcgg ccatgttgta
    28801 cgccaacggg cgcgtggcgg atgccatcag tctgctgcag atgttcctgt tgcagacccc
    28861 gaacgaaagc ggtctgtggc tgatgctgtt cgatttgtac aatatcaccg gacagcagga
    28921 agaattcgaa aagctcgcac tggattacac gatccggcgc gagcagtcgc cgccggcatg
    28981 gaaagacagg ttggtcaatg gcaacgacat gccaggactg acactgctgc agcccggcgc
    29041 gacgggcaac ggtcagctgt tcagcctgaa ggggcggctc gacatcacac tggccgagcg
    29101 catccagctg ttgcgcgagg cggcagcggt gggggcgatc cagatcgatc tgtcgggcat
    29161 cagtgcggtc actccggaag gttgcgaatt gttgcagttt gcgctggccg acctgcagaa
    29221 acagggggtg cggctacaga ttgccagtgg cgcgctgatc ggtctgctgc agcaatatgt
    29281 ggccagaccc gaagaagctg cggcgccgca gtacttgctg ctgttgctgc agttgtacca
    29341 gatgcagggc aagctggccg agttcgagga tctggccgtc tcctatgccg tccagttcga
    29401 ggtctcgccg ccgtcgtggg aagcgccacg tcagccggtg cgcatcgtcg ccggagccga
    29461 gccccaccac gacgcggacg tgccgcaacc gggcaatgta ttttccctgc gcggtgtgct
    29521 cggtgccaga tcgacggcag tgctggccga actgcgcaat tttgcagccg gccaggctga
    29581 cgtggtgctc gatatggcgt cggtcgaccg catcgaattt tccagcgtca gcatgctgat
    29641 ggaaacgctg atctctcttt ccgccctggg caagaagatc agtattgcac actgcaacat
    29701 gctggtatac gtgttgctgg tggtcatggg catcgaccag attgcgcagg tcgttgcgcc
    29761 cgtgtacaaa taagacaggg ccacgcaatt cgaatcattg cccggcctgc ggctgctgtt
    29821 ccgaacgtcc ggtttcaagt tgccgcaccc gtatcccggc gccgtcaccg ttcgggaatg
    29881 tgtgaggcat ggcagactga acgaggataa caggatggat acgaccagca cccctacatt
    29941 tc
//
LOCUS       DAIANL010000027        10151 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_634698, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000027 DAIANL010000000
VERSION     DAIANL010000027.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634698"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2002 a         3326 c         2982 g         1841 t
ORIGIN      
        1 cctgccatgg tggttgttcg acatgcgccc ccaaggatat ctcgggcggg cgtacgcagc
       61 ccgccatggt gctgaacttg gactaccgga gcggatcacc gactggagcg attcccacgc
      121 cttgcgagca ctacttgcgc atggacatga tgtggtagga aatctcctgt taggcgatat
      181 tgcgcgggat cgattccttg ctgcacctgt gtctgacccc atcaaagagg aacagaaggc
      241 cgaagagtat gcccggttgg cgctggaagc tgcccgcggt gagctaacag gatcgtcagc
      301 cgggggagaa cagccgaagt tcactgccta tgccctgacg tccgatgagg cccggcacgt
      361 tatcgtgaag ttcagcgagt tggaggaaag ccccgtcagc gaacgctggt gcgatttgct
      421 gctggccgaa catttagcgc tggacacatt gcgagaagcc ggtatccccg ccgccaggac
      481 gcggatattg gaccacaatg gtcagcgctt tctggaggtc gagcgctttg atcgcatcaa
      541 cgaactgggc cgacgtgcgc ttatttccct ggccgcgatg gatgctgaat tcgcaggaac
      601 cggaaaaggg gtctggccaa tcattgcgca ccaacttgct gccaccggcc acatccactc
      661 ggaagccgcg gacagtgcag ccatcctgtg ggcctttggc acactgatcg gcaactccga
      721 tatgcacaac ggcaacctgt catttgtttc cgagcatggt cggccttacg acattgcacc
      781 agcctacgac atgacgccaa tggcctttgc acctcgtagt ggcggcggaa tacctgacag
      841 cctccctgag gcgacgattc acgccagtgt ggccaatgaa acctggaggc gcgcacaaga
      901 actggcgcgc acgctcctcg cccggatcag ggtcacaaca ggatttagcc agcgatttgg
      961 gtcatgcatc gtcgcgatgg aacaacacat cgagacggcc agtcgcaaga tcggacgact
     1021 ggagtgagtc tggcgcaaac cacatcggaa ccgggtggct gaccgactct acacgctgcc
     1081 cgggggcggc catggctggg aagcattggg ggtggtcaag gttctggcag gtttgcggtg
     1141 cggttcacct cccgggcaga ttgcctgcag catccgggaa acacagcgcg tggcatacgg
     1201 cctccagcat gggcaccagc tccattgcac gtaacacatg ctgccgtaac gtcgccatgt
     1261 cttccgtata ttcatgcacc agcagattgc gtaggctgcg cgcttcgatc caatcggcga
     1321 cgctcggtag cacaccaagt ttttcggcac gattgagatt atccagcacc gtaccgacag
     1381 gttccagatg cacattcagc cagacgggta acagtttgtc ccccagcgta tcctgcaaac
     1441 gcgaaaaacg gctcacaaac gcgtccagac gctcagtcag cgctgcgttc tggtcaacag
     1501 catccagcca gccgaactcg ggatcggctc ggctcaaccc gtgcgccgaa tacagcagat
     1561 actgacgttc cctggcggca acctgccgca aatgcgccag tcttgccagt tgtgccagat
     1621 atgctgcgct gtcgctcaca ggcgcactcc ggtggcaagg gcatgccgat ggatcggctc
     1681 cagtacagtc aatggatcga tgaccagcac atcgaatttt tgcatgccca gtttttgttg
     1741 cagcaatgcg ttgtatcggg cggacaggcg gcctttgtcc gcaacgggtg catcgagctg
     1801 gatcaacaca tcgatatcac cacctttggc agcatcatcg aggcgggaac caaacagcac
     1861 aacctcagcc tgcgggccga acacctgccc ggcaagacgg gtcaggacgg attgataatc
     1921 aggtggcaga cgcattatgc tcacttttgc gaagcaaatc ccacaccgcg gcgttggcag
     1981 aggcaacgcg accatccctg tatcatggtg accgtgcgac gaaatgtcaa cacgacccgg
     2041 atgatgcctt gcccgtggcc acgaggcaca ccacgaaacg aacatcccgc gtaacgcgcc
     2101 aggcttctgt ctcgtggacg ggactcgcct ctgctacacg cgccgcccaa ctgacgccac
     2161 acatacgccc gggactgctg cgcaggctat tttgcctcgg gcggtgccat gcgctgcaca
     2221 tagcgaaaca cgaggccacg cccttgcggg ttgtccatca gcgagacctg cccacccaga
     2281 cgtgccgccg cttcgcgcac gatcgccaga cccagcccgc tgccgggcac atccgtgttt
     2341 tccacacggt gaaacgggtt gaacacctgt tcgcgttcgg cgggcggaat gcccgggccg
     2401 ttgtcagcca cgtcaatcac cgcatcatca cccagccgtt gcagccgcag ggtaaccgtg
     2461 cctccttcgg gcgtgtattt cagcgcattg tccagcgcat tgaacacaat cagccgcacg
     2521 ctctgcgtat cagccagaat ttccggcggt gccagatctt cgaggccgag atcgatgtgc
     2581 cgggcttcgg ccacgggcag caactcggcc acgatttccc gcgccagtgc agacagttgc
     2641 acccgctgct ttttttcttc tccggcctgg agcctggcca gatccagcag ttgcgccgtc
     2701 agtacctggg cacgctcgat tcccgcccgc agcggtacca gccgttcctg caccgcttcc
     2761 atgtcgtgcg cccgcgcaag attctgtgcc tgaaacgaca gtgccgccag cggcgtgcgc
     2821 aattcgtgcg aagcatcagc gacgaagcgt cgttgttcgg cgaccaggat attggcccgg
     2881 cccagcaggc ggttgatcgc ctgcacgaat ggcacgattt ctgcgggcag ttcctgcacg
     2941 ggcaacggcg acagctggtt ggccagctga ttgtccagtt gatcggacag gtgctttacc
     3001 ggtttgagtt cgctgcggac gatctgcacg atcagccccg caagaatcgg caacaggatc
     3061 aacaggggga tcaaggtgcg caaggcgctg ttcattgcaa tttcgtcgcg gctttcggta
     3121 gactggaaca ccgcgatgcg gttgccggca gccgcttccc gcacgtacac ccgctgcaca
     3181 ccaacgtcct ctccaccatt cagcgtatga aaacccggag ccaacccgtg cggcatccag
     3241 acaggcttcg gctcctgcgg cagatggatc acccggatca acgaatcctc atcgtcactt
     3301 ccgttgtcgg ccgcagacag acgcgcgttt ccgtgccggt atgcgccatt cagtatggcc
     3361 acctggcgca aagcgtcatc ctggaattct tcggcctcgc cataggaaaa ataaaacgat
     3421 actgccgttg ccacaatgcc cgccaccaga atggccaagg ccagcgagcg cgtcagatgg
     3481 ctttgaatag aacgtttcat gcagccccac ccttgtatcg ttgtttgaca ttttccctgg
     3541 cccgggccgg atgaggctgt cagaacccag ttcaccgttg cggccaggcg cagcgtccgg
     3601 tgaccaggcc gaggcagtcg atgacgtgac agacgttgcc actgcgcccg ccgccatcag
     3661 gtacgcggct tgtcaaccag ccagcccgcc ccgcgtatat tcttgatgac atcgccgccc
     3721 agtttcttgc ggatgctgtg tatcagaaaa tcgaccacat tgctttcgac ttcttcattc
     3781 cacccataaa tccgttcttc cagcgccgtg cggctgaaga tcgtgcccgg cgcaagcagc
     3841 agggtgtgca ataacgcaaa ctcgcgcgcg gacagcgtca cggtcacgtc gccatgatgc
     3901 gcttcgtgcg aggccgggtc caggctcacg gtgccattgg taagccacgg cgcagcccgg
     3961 ccgccgtgac gccggacgac cgcccgcaac cgggcaatga gttcgttcag gtcaaacggc
     4021 ttgatcatgt aatcatcggc acccagatcc agccccttga tccggtcctg gatactgtcg
     4081 cgtgcggtga tgatgatgac cgggaggtca ttgccagcct gtctcaggcg atgcagcaat
     4141 tcaaatccgt cgcgacgcgg caggccaaga tccagcagca tggcctgata ttcgccggtc
     4201 tcgatcacct gcgcagcggt gacgccatcc ttgacccagt cgaccgcaca ggcagaatcc
     4261 cggagaaaca gcgacactgc ctcgccaatc atggcgtcgt cttcaaccag caggatacgc
     4321 acgtcagaga atcctccgca taaaaaagac cgttccggag atgatattcc ggtaacggtc
     4381 ggggtcatgc tgccgggttc acatcaatcc gaatcggggc cttcggtgct gttttccagg
     4441 accgcgccgg ttttggcatc cacgccgact tctctggtgg ccgatccgtt ccggatatcg
     4501 aacgagtagc gcaacccgct accgccgctc tctttctcca gctcttcatc cacgatcttg
     4561 ccagggctgg ccttgagcgc aatttcgcgg gcctgctgga tgctgatttt tgcgtcaccc
     4621 tgaaattgct cgcccgaata cgccatggct gccgaagcgc ctgtcgccag cacgcacacc
     4681 agacccagag caatcacctt ccgttgtttc atgttcgact ccttcatgtg aggttcagac
     4741 gggccggaac atttccgacc tgacgaacac agcataacga agcagaatta ggtgccgatg
     4801 aggcaatgtg cagttgctct ggttccggat tcctgcagta taacggttcg ggcgaatgat
     4861 ggccgcccgg ctcaggcccg aattgaacag gaccggtctt tgcaccggcc tgaacatgtt
     4921 tgccggagcg ttatctgcga tcgatgccgg atcatgcgaa gcagaaacga agctgccaga
     4981 acggcatgcc gatctggcca aagccgcagc aatcacggga ctgccgatcc tggacgcaca
     5041 ccgactgcgc tatttatcct gccatttgac cacatgcacc ggattcaggc tgcggccgag
     5101 gaaacgctcg ccgatggtct ggaattttac cggcacatcg gtcacgtaat actcgtagcg
     5161 cgatggggtg ctgccggggt tgagcaggct gcgctcggcg agcagcgcgg cagtcaggtt
     5221 ggccatggct tcagccgaat cgaccaggcg cacgtcctcg cccaccacct ggcgcaacaa
     5281 cggcttgatc agcggataat gcgtgcagcc gagcaccagc gtgtccacgt gctcggccat
     5341 caccggccgc aggtattctt ccgcagtaag ccgggtgact tcatggtcca gccagccttc
     5401 ttccaccagc ggcacaaaca gcgggcacgc ctgcgaatag atgcgcgcct cgcgctgctg
     5461 tgcatggatg gcgcgcgcgt aggcattgct gttgaccgtg gtcggcgtgc cgatcacacc
     5521 gatccgcccg cccgggctgg cctgcgccgc ggcccaggct cccgcctcga tcacatcgag
     5581 taccggcacc ggggacatgt cacggaccgt ctgcgccgcc acggcggcca tggtattgca
     5641 ggcgatgatc agcgtcttga cgtttttttc cagcaggaac tgtgcgatct gtgtcgtgta
     5701 gtgggcgatg gtctccaccg acttcacgcc gtaaggcacg cgcgcggtat cgccgaaata
     5761 gacgatatgc tcgaacggca ggcgttccat cagcgcccgc accacggtca gcccaccgat
     5821 gccggaatcg aacacgccta tgggctgcga agggtcgatc tgcatgacaa cacccgaatc
     5881 aaatgcccca tgctagcaca ggcggccccc gctccgcacg ccccccgctg cgcagccggc
     5941 gcggaaggtc caccggctca gggcgaaacc gctgtcggca acccgccctg ccgctcaagc
     6001 caggcgacca caccgtccag aattgccagc aaagaccgcg ctgcctcgat agcccggttc
     6061 aatatcgcag cctgcacggc aggatcatca ggataatctt gcgcgaatgc attgcgcaat
     6121 tcgcgcaggc ggatccactc gtcggccgag gcgatgacgc caattttttc caggcgggcc
     6181 agtttatcca gaaaagccga tagatcgcca ggttcatgcg cccgatccag cacggcagga
     6241 aacaggcttt gccccatgac atccagcaaa cgggcaaacc gtccagtgaa ctgatccagc
     6301 accgccagat ccatgtcgct cagaccggcc aaccgttggg catcgaacgg aacactgaca
     6361 gacagctgtt gcatcgccca ccgcaatcgg tctgcgtggc gacggcacac tgcaagtatc
     6421 ggctccagaa cctgcgtcat gtgagttcca ccccggtgct gcgcgcaagg cgccagatcg
     6481 gcacatccgc agtggactgg gcgcgacgca cggccatatc gatcttgcgt tcacccagtt
     6541 gtctgtgcag gcgggccagc acaccgagcc tggcttccac gacctgttcc gcatcgctga
     6601 ttgccgactc cacgtacagg tcgatgtccc ctccgcgcgc ggtatcatcg acgcgggaac
     6661 cgaacagcca gacccggctg tcgctgccga acgcctgccg cacggcagac acgatttccg
     6721 tccgctgttg ttcgctcaaa cgcatctccg ctccgccggt tgacaccgaa tgccggtacc
     6781 ggacatgcta tcatgggaca ttgagccgtt ccaacacgca gcacacaaga tgccgtgtgt
     6841 tcgattgcct cggactgatg cccatgcccc aaccctcatc tccccgatca tccaccgcac
     6901 tacagcgcca tctcgccagc gagaccgaca cgctgcgcct gggcgcccag cttgcgcggg
     6961 cgttgcgtcc gggcctgatg gtgtacctca gcggcgacct cggcgcaggc aagaccacgc
     7021 tggtgcgcgg actgctgcgc gagatgggct ggagcggccc ggtcaagagt ccgacctacg
     7081 ccctggtcga gatatacagt ttgtttaact tcgacttata ccactttgat ttatatcgct
     7141 tcaataatgc gctagaatgg gaggacagcg ggtttggaga atactgccat tccggcagcg
     7201 tgtgcctggt cgaatggccg gaaaaggcgg ccggtgtgct gcccgatgcg gatctgcgca
     7261 tcgatctcca gccccgagac gacacgcgcg acgcgcagat tctcgccctg accgccagag
     7321 gtgaagcatg cttgcacgaa ttgcccgatt tctgaaactg gcccgactgc ccgtactggg
     7381 cgggctgctg ctgctcggcg gtctgccggc gctggccagc aacacgctgc aggcggtgcg
     7441 ctactggcca tcgctcgatt acagccggct caccttcgaa tcgcctgcgc cgctgcatta
     7501 cacgctgttt acgctcgacc atcctgcccg gctggtgctc gacctgcaag acgtcgcatc
     7561 cactgcagcg ttgcaggccg tgagcgacga catcaatcac ggcggtgatc cgtggatcag
     7621 caagatgcgt tttgccgtca accgtcccgg cgtggtacgg ctggtgctcg atctgcgcga
     7681 taccgtacgg cccagcgcat tcagcctgca gcctgtcggc gagtacgcct ggcggctggt
     7741 gctcgatgtc tatccgggcg gcgcgcaagg cacgaacggc gccgcaccgc cggctgccca
     7801 ggtgacgccg gagattccgg cgcatccggc gacagcggat accggaacgc ctccggctgg
     7861 ggctgccgtg ctgccaccat cgcccactcc cgtccagtca gacgaggcat tgaccgcaac
     7921 cggccgcagc caccatcgcg cagacggcag cagcgatacg cccatcatca cgcgactcat
     7981 caccgtggcg atcgatccgg gccatggcgg cgaagacccc ggcgcgcacg gcgaaggcgg
     8041 ttcactggag aaaaacgtca cgctggccat cgcacgccgg gtgcaggcca aactcaatgc
     8101 tgtcgacaac atgcatgccg tgctcacgcg cgacagcgat tacttcattc cgctggccga
     8161 gcgggtcaac aaggcgcgcc gcatgaatgc cgacctgttc gtctccatcc acgccgatgc
     8221 atacatttca ccggacgcac gcggttcgag cgtgttcacg ctgtccgagc acggtgcgag
     8281 cagcgctgcc gcacgctggc tggccaaatc ggaaaacgat gccgatctgg tgggcggcat
     8341 caatgtcgcc accaaagacc gcgatctggc acggaccctg atcgacctgt cgcagaccgc
     8401 gaccaacaac gacagccggc gactggcgca cgacgtgctc gaccagttgt ccgaggtcaa
     8461 ccagttgcac cacggtggca tcgaacaggc gggctttgcc gtgctcaagg caccggatat
     8521 tccgtcgatt ctggtggaaa ccgcgttcat ctccaaccca caggaagaaa aacgtcttga
     8581 tgacgatgcc tatcaggaca agatcgccgg cgccatcgcg caaggcatcc tgcgttatct
     8641 cgccagcaat ccggccacgg tgcgcggcaa gctggcggtg cgcgattagc gccgccgtca
     8701 ttgacttcac agcaaggatg cttctggcac catgtccgcg attcgcctgc ttcccgatgt
     8761 actgatcagc cagattgccg ccggtgaagt cgtcgaacgt cccgcgtcgg cactcaagga
     8821 gctgctggaa aacagcctcg acgcaggcgc caccgaactg cgtatccaga tcgaagatgg
     8881 cggcatgcag ctcattcgcg tcagcgacaa cgggcgcggc attcctgccg accagatcgg
     8941 ccttgccatc gcacgccatg ccaccagcaa gatcaccagc ctggacgatc tcgaacgggt
     9001 gggcagcttc ggcttccgtg gcgaggcgct ggccagtatt gccgccgtat cgcgcctacg
     9061 actggtcagc cgcacgcagg aagagccgca tgccagcgaa ctcggctgtg agggcggtcg
     9121 cccgacgcga cttgcgccgg ctgcgctgac tggcggcacg ctgatcgaag tgcgcgacct
     9181 gtatttcaat accccggcgc gacgcaagtt cctcaagacc gccgccaccg aatgggcgca
     9241 ttgcagcaca gtggtggaac gcattgcgct tgcgcgcccg gatgtgagca ttcacctcga
     9301 acacaacggc cgcacggcgc tgaaactgcg tgagcagtcg ttgtccgaac gcatgcacag
     9361 cgtgctgggc gagcgctttg ccgccatggc catcgaggtc gacgaacccg gcccgccgct
     9421 tgcgctggcc ggcctgctgg cccggcccca gccgggtgaa tccggtctgc aggacgcgca
     9481 atacctgttc gtcaacggcc gtttcgtgcg cgacaaactg atccagcatg cgctgcgcca
     9541 ggcgtggcag gatgtgctgc accacgacca gaaagccggc tggtgcctgt tcctgcacat
     9601 cgatccggcc accgtggatg tcaatgtcca tccggcaaaa accgaaatcc gctttcggga
     9661 tggccaggca gtgcaccgtt tcgtgtatca cagcgtgcac cgcgcgctgt cgcgcccggc
     9721 tggcgctgcc cccgcaagtg gacaacagcc tgtcgcaggc agcggcgccg gtgcgcctgg
     9781 ccccggcagc ccggcaatga cacgcgcgcc gcagcagcag acatcgctgg ccgggctggt
     9841 gaccgagccg acatcgcgct acggctggac gcaggccgcc acgcctgcgc acactgcgac
     9901 cgcacacacg ccgttcatcg cgcacgctgc aaccgcaacg gaggacgtgc cgccgctggg
     9961 ctttgccctg gcacaactgc atggcgtcta catcctggcg caaaaccgtg acgggctggt
    10021 ggtggtggac atgcatgccg cacacgagcg catcgtctac gaacggctgc gtcagtcgtt
    10081 cgaccgccag ggcatgccga gccagccgct actgatcccg ctggtggtgc aggccgatgc
    10141 gctcacgctg g
//
LOCUS       DAIANL010000028         7594 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_839020, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000028 DAIANL010000000
VERSION     DAIANL010000028.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7594)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839020"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1753 a         2305 c         2043 g         1493 t
ORIGIN      
        1 tgccggcttc cctaaacgcg acgaaagtga atttgatacc ttcggcacgg gacattccag
       61 cacctcgatt tctgccgcac tgggtatggc cgtcgccgcc caactggacg gtctggagcg
      121 tcgcgcggtt gccatcatcg gtgatggcgc gatgacagca ggcatggcct tcgaagcttt
      181 gaacaatgcc ggcgacatgg atgccaacct gctggtgatt ctcaacgaca atgacatgtc
      241 catctcgccc aacgtgggtg cgctcaacaa ctatctggcc aaactgatgt ccggccgctt
      301 ctacgctgcc gcacgacgcg ccagcgaaaa gatgctctcc catgtgccgc ctgtgctcga
      361 actcgccaaa cgcgccgaag agcacgtcaa gggcatggtc acgccgggga cattgttcga
      421 ggaattcggt ttcaattaca tcggcccgat cgacggtcac gacctcgatg tgctggtaga
      481 cacgctggtc aacatccgtc ggctggaagg accgcaattc ctgcatgtgg tcacgcgcaa
      541 gggcaagggc taccatttcg ccgaagaaaa cccctgcctg taccatggcg ttggatcatt
      601 ccgccccgaa gccggcatcg aggaacgggc cggcgggccg gccacctaca cacgcatttt
      661 cagcgactgg ctgtgcgaca tggccgaaca ggaaccggcg ctggtcggca tcaccccggc
      721 aatgcgcgaa ggctcgggcc tggtgcgttt ttccgagcgt taccccgacc gctatttcga
      781 tgtcggcatt gccgagcagc acgcactgac ctttgccgcc gggctggccg cagagggcct
      841 caaaccggtg gtcgccatct actcgacttt cctgcagcgc gcctacgacc agctgatcca
      901 cgacatcgcc ttgcagaacc tgcctgtggt attcgccatc gatcgtgccg gactggtcgg
      961 cgccgacggt ccgacccatg ccggaagcta tgacctgagt ttcctgcgct gcatccccaa
     1021 cctggtcatc atgacgcctt ccgacgaaaa cgaatgccgc cagatgttgt acactgccac
     1081 gcagctcggc acgccctgcg ccgtccgcta tccgcgtggc accggaccag gtgcccacgt
     1141 cgaacaggaa atgacagcac tgccaaccgg ccgcggcctg ttgttgcgcg aaggcatacg
     1201 cattgctgtg ctggcgttcg gcagcatggt gcggccgtgc cgcacggtgg ccgagcagct
     1261 cgatgccagc ctggccgaca tgcgcttcgt caagccgctc gacgccacac tgatcgggca
     1321 gctcgcgcgt tcacacgacc tgctggtcac cgtggaagaa aatgccatca tgggcggcgc
     1381 cggcagtgcc gtggcagaaa ccctcaacgc cctggcgctg accgtgccac tgctacaact
     1441 gggcctgccc gacgcattcg tcgaacaggg cgagcatgtc cagctcctca gccagtgcgg
     1501 ccttgatgcg gccggcatcc tgcacagcat tgaaactttt cgcaggcaaa attatccgag
     1561 cagttaactc ggaaatgaaa tcggcgtaca ctgatacgca ccaatccggc atcattgcct
     1621 ccaaccagtg agaatgcaac ccatgaacca atgctgcgaa accaccagca tgcccgatgt
     1681 acaaaactat gccgacagcc ggcaaatcgc catcgacaaa gtcggcatca agtccatccg
     1741 ccacccgatc cgcatcgccg acaaatccgg cggcgcgcag cacaccattg ccaacttcaa
     1801 catgtacgtc tacctgccgc acaacttcaa aggtacgcac atgtcgcgtt tcgttgaaat
     1861 cctcaacgcc aatgaacgtg aaatttccat cgaaaacttc gaaggcatgc tgcgacaaat
     1921 gaccgagcgg ctcgaagccg attcaggtta catcgaaatg agcttcccct acttcatcaa
     1981 caaggctgcc ccgatctcgg gcgtgcacag cctgatggac tatgacgtga cctttatcgg
     2041 cgcaatcgag aacggccgtt acgtgcatac cacgcgcgtc gtcatcccgg tcaccagcct
     2101 gtgtccgtgc tcgaaaaaga tatccagtta tggtgcgcac aaccagcgtt cacatgtgac
     2161 catcagtgcg cgcaccaacg attttgtctg gatcgaggat atcgtcgctg tggcggaaag
     2221 ccaggcctcc tgcgaactgt acgggctgct caagcgtccc gacgagaaat acgtcaccga
     2281 acgcgcctac gacaacccga aattcgtcga ggacatggta cgcgacgtgg ctgcggcact
     2341 cgataacgat ccgcgcatcg accgttacac cgtagaaagc gaaaatttcg aatccatcca
     2401 caaccattcg gcctacgcgc tgatcgagcg cgacaagacc gcaggccgga cggcatgaaa
     2461 ccggttgcca ttttccgcca cattccgcac gaaggcgctg gctacttcgg tgaattcctt
     2521 gcgcgtcacg gtattccgat gcaactcgtt cgcatcgatg ccaacgagcc ggtgccagcg
     2581 gataccgggg ccttctccgg tctggttttc atgggcggca gcatgagcgt caatgacccg
     2641 ctgccatgga tcccgcattc gctcgagctc atccgccagg cggtggcctg tggcattccc
     2701 gtactcggtc actgtctcgg cggccaactg atgtcgcggg cactgggcgc agcggtcacg
     2761 cgcaacccgg tcaaggaaat cggctggggc gcggtgagtg ccgaagacag cgatcttgca
     2821 cgcgaatggc ttggaaacat acgcagcttc gattcgtttc actggcacgg cgaaactttt
     2881 gcactgcccg cggacgctac gcgtattctt ggcagcgcct attgcaccaa ccaggcctgg
     2941 gttgcagaca accgccacct ggccatgcaa tgtcacgtcg agatgaccga ggacatgatt
     3001 cgcagctggt gcgaacatgg cgcgcaggaa ctggccgaag ccagcaccag tccggccgtg
     3061 caaagcgcgc aggcgatgca ggccggcatg acggaaaaac tggccgcact gcacggcatg
     3121 gccgaccggc tctacgaacg ctggcttggt ggcgtgaccc ggcaatgaaa gcacccttcc
     3181 agcctgaagc cctgcacacc ctcatcgggc gacagtttgt gctggatggc gaacgctata
     3241 ccgccatcga gattctcaac gagccacttg cgctcgtcgc gcagaaaaat cagctcgatc
     3301 cgcactacca gtacgacgcc tatggccgtg cccgcagcca ggtccacacc agcacactga
     3361 ccattcctgt gctcgctgca gatggcacca gcctgcacgc cgactttctg cgcatctcca
     3421 gctggatttc ttcctgaacc agccacctcc gcagcttgac gaatccgaca gattccgtat
     3481 aatccacaag cttgataaac cgctgaaaca cgttgcgact actaccgatg cacccggcat
     3541 gaccggcagc tccctgcaag gcacccgcct ttcgcacagc aacagcgcaa aggcgtaccg
     3601 gaacggattt tggcgttttg tcctgccggg tcggtagctc agccggtaga gcagcggact
     3661 tttaatccgt tggtcccggg ttcgaatccc ggccgaccca ccacgacaag aatcattcct
     3721 gacgggacca ttcttgtggt ggtctccagc agtagcgcac gccagcagcg tgcacatggc
     3781 aacacgatca ggcatgggtt gttagctcag ttggtagagc agcggactct taatccgtag
     3841 gtcgtcagtt cgaatctgac acaacccacc aataaaatca atagcttacg agatgtcgca
     3901 ggctattttt cgtccagatg gtttttgctg caatttgctg caatggccct ttgacaaatt
     3961 gctataactg tctttaaccg gagaaaaaga caggcatggc aagcatcctc aaacacggcc
     4021 cgtatcgatt tcaggtcacc atccgtcccc aaggctttca gagccaatcc ggaacattcg
     4081 aaagctggcg cagaacctgt gcatggggga tcgccaatca gcgcgcagac tcgccatccg
     4141 tcttttggca aaaccatgcg cagcaggctt tctgatctgt gccactgata ctgaacatga
     4201 tgaaaaccgg aaactcgggg ccctgtttca caccggagga cggtagctcg cgcagacatc
     4261 aaaccatgac ccgactctga gctggcctcc gcgcgcatcg cgcaatgcgt accagactga
     4321 taaatctgac aaaatacgcc agcaaaacct ttacattcaa tttgcgtagt atagaatcgc
     4381 ggggaatatg cctatttcct gacgatacgc actcatgacc acagccagct caccgctgat
     4441 ccgcatgatg gagtttacga accttcccac attgccgcat atcctgttgc aggttctgga
     4501 aatatgcaat cgtgacgacg cttcattaag tgccatagcg gagctgatca gcaaggacgc
     4561 tgccttgagt tccagggtga tgggtgccag caacacgccc cgtttcgtca agcacagcaa
     4621 gttcatatcg ctggaacaca gtctctccct gctgggtctg gatatcgtca agaccatggc
     4681 catcagttcc tcgttctatc aggtattcaa cacgctcggc accggccccg gatttgattt
     4741 caagaccttc tggggcagat cgctgaccca ggcggtgctg tcccgactgc tggcgcagga
     4801 gacggggtat gcgcatcgcg aagaagccta tttgaccggt ctgctgctgg atatcggcca
     4861 gctggtactg tggagcaact ttccccggca atatgcgcag ctgttgagcg aatcagccaa
     4921 tgatgcccac ctcatgacgc ttgaatccga aaagctgggc aatactcact gcgaagtcgg
     4981 ttcatggctg gtcagcaact ggaacctgaa ttctttcatc gcggacgcgg tactattcca
     5041 ccatttgcca caagaaaaag ttgcgggcgc gcacccgcta atcgtgatcg ctcaggttgc
     5101 aaaccgcctg acgatgctgc acgccgatga cacggccaca ctgggcgaga tagaaacctt
     5161 gttggaattt cctgccgcca gactgcggca actgctcgat gattcaggga aaatcgtcga
     5221 cgacgtcgcg cagtcactcg gcattgaaat cgacacttcc ctgttcaggc agcattcgca
     5281 gctcaacagc gctgcgcccg acaacatgcg tttgcaaaaa tcccgtctgg cgacagagtt
     5341 gcgcgacaat gtgatggtgg gcaggaatcc gttcggtgcg ggcccaccca cctcgctgga
     5401 cgacacactg gcaaccgtgc aacgatcggt caacatcctg tttgggatcc aggagatggc
     5461 ttttttcttt cccgacccat cgcaccagcg tctgcaaggg aaatgtcttt ccgaacaaag
     5521 cctgctgatc aatgaaatct ccgtttcgct ccgacacggc gacggtctga tcggcaaggc
     5581 attttccgga cggaaagccg tagcttcttt cgacgaaccc gacaccgtcg ctgcgagcat
     5641 cccggatcgc caggtgatac gattgctggg cagggaaggc atatattgcc agccgatttt
     5701 tactcggacg acgggtgtcg gtgtcctggt gttcggcatc cacccgtcgc aactgacgca
     5761 gatccgcaaa caggacaaac tggtatccat gtttgcccag caggccgccc aggccattgc
     5821 actcgtcaac acccttgccg aacaggaatc gcgcatcaag gccgagatga ccgctcaggc
     5881 gcgcgatcag gcgcgccatc tggtgcacga agccaacaat ccgctgggca tcatcaagaa
     5941 ttacattcac ttgctggaag gcaaactgtc tgcagaagac agtgcacgca gcgaactgaa
     6001 aatcatccag gatgaaatcg accgggtgtc acgcatcatc aaaacggtgg ccacggctga
     6061 tgctgcagcc ctgcctgaga atcaggtgat caacgtgaat gaagtgatcc gtagcgtcta
     6121 tacactcact ttcgaaccgc tgttcgcacg caaccggatt catgccgaaa tccggcttga
     6181 cccgaacatc ccgaacgttg ctgtcaaccg tgacaaactg atccagattc tgatgaatct
     6241 gctcaagaat gcggccgaag ccctgcagca cggtggatcg gtacgcattg ccacccatgc
     6301 caatgtccgg catgaaggca ggaaccacat tgaaatccag ctgaaggatg atgggccagg
     6361 aatgacgcaa tatgtcaggg acaacctgtt cactccgctt gccagcacca aaggaacaga
     6421 acatgccgga ctcggactgt cgatagtcaa acgcatcata gatgaaatgg gtggatggat
     6481 ctcctgtcag agcgatgaag cctcaggcac ggctttccag atcctgctgc ctgtggatgg
     6541 cggcccggcg gacaccgcac agcccggatc atccaggcaa agataaggat ttaaaatcat
     6601 gaatgctgtt gcaccccacc cgtcggttct gatcgttgat gatgagccac tgctgcgcaa
     6661 cagcctgttt gccatactga acacccacgg ctacatgcca gcgactgccg atggcggcgc
     6721 ctctgccctg aatgcgcttc aggatggaaa atacgaactg atgttgctgg atctgtgcat
     6781 gcccggtatc gatgggcatc aggtcatgga gtggatgggt cagcatcgca tcgacacctg
     6841 cgtgatggtc atcagcggtg acgtatcaat cgactccgcg atcaacgcat tgcgtcatgg
     6901 cgcctgtgac tatctcagaa aaccttacga aatcgacgaa ctgttgctca ggatcgaaaa
     6961 agcactgtcc cgacgcaaac tggagcagga gcatcggtcg gtcaatcagc gactcaggga
     7021 ttccgagcgc tggtatcgct acatggtaaa cagttcaccg gatttcattt atacgctgga
     7081 tgccgaaggc gtatgcacgt tctgcaacga ccgcgtgcaa tcgctgctgg gttacagcaa
     7141 ggaaagcatg atcgggaaac ccctgacagc cttcatctat ccggaagatc tggaagccgc
     7201 gcaatacatt ctgcaggaaa aacgcagcgg cgaccggggc agcggcaatg tggaaatcag
     7261 gattccacac ggcatcgatt cgacattgct gacactggaa ttcaattctt tcggcatgta
     7321 cgaagaaagc gaagccgggc tgccgtcccg gtacgtaggc gtttacggcg tcgccaaaga
     7381 catcaccgaa aagaaaaaga acaccgatct gatcatctac caggtgtatc acgatcagct
     7441 gaccggactg gcgaatcgca aactgttcaa ggatcacctg gaactggcga ttgctcaggc
     7501 tcgacgtcac cagcacatgc tggcgctgat gttcctcgat ctggatcgct tcaaggtcgt
     7561 caatgacacg ctgggacacg ccgttggaga ccgg
//
LOCUS       DAIANL010000029        13510 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_226437, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000029 DAIANL010000000
VERSION     DAIANL010000029.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 13510)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13510)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13510
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226437"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2731 a         4475 c         3896 g         2408 t
ORIGIN      
        1 gtgccaccag acggtgcaac tggtcttccg tcagttctgg cacctcgtta tgcacgatgc
       61 gccgctcgat cacttcggtc ggatagtcga actgtatgcc gacaaagcgc tgtttggtcg
      121 acggtttgag atccttgagc acggtctggt agccgggatt gtaggaaatc accagcatga
      181 aatcttcatg cgcctcgatc tcctggccct ttttttccag cggaagctga cgacggtggt
      241 cggtcagcgg atggatgatc accgtactgt cggtgcgtgc ctcgacgatt tcgtccaggt
      301 aacagatcgc accggccttg accgctcgcg tcaacggccc gtcctgccac accgtctggt
      361 cgccttcaag caggaatcgt ccgacaagat cggcgctggt cagatcttcg tggcaggaca
      421 cggtcaccag cggccggcgc agccgccagg ccatatgctc aagaaaacgc gtcttgccgc
      481 aaccggtcgg ccccttgagc atcaccggca gacgcttgcg ccaggccgct tcgaacagga
      541 cgatctcatt gccctgcggt tcgtaatacg gttcggctgc agcatcttcc tgaggcgcct
      601 gaataatgtc gcgctcgaca ccgcgcaacc ggttcaacaa tgctttcatg acatctccag
      661 acgcgattcg cgcatgcagc gcgacttcat gaacaaaaac agatgccgca atgtacgtac
      721 ggtcatcttt tgcggatgcg caggaagcgc atccgcccga tccatctgca gacaattgtg
      781 gtagtacacc agttcacggc agttgtcgcg aatggtgttc cacgatgggt cacccttgac
      841 cagtacgcgc aggcattcga gcagatcggc atcgtctgcc ggctgcgatg cctcacctac
      901 atcggcgata taggcagtga tcaactggcg cacgatacgg gtcgcctgtt ccacggtttc
      961 ggccagagga gtgtcctgcg ctgatgccag ggcgatttcc atcgcaccaa tctgctggtc
     1021 ggcctgatcg aacgtgtgtt gcaacaaagg atggttcatg ctttgactag acccatgaca
     1081 agtttgagaa tacaatcttc gactgttgac gcataatagc cgatcatttt actcaacttt
     1141 agcgacaaat ggaatgacaa catgaaaaag ctggtcaaac tcgtcaagct gaacaacctg
     1201 gaatacaacg ccaaccgtga tcctgccgtc taccgccgca gcgcaaacga aaacttccgc
     1261 atccaggcac tgatcgacgc cagcggtacg gtggaatgcg cgctgaccga cagcagcggc
     1321 aaggtcctca acaagcaagc cgtaagtgct ccgggcacct atacccagga actggggttc
     1381 gccacccccg gcgtacgtgt ggtgacgctt tccgtcaccg gtcaggggca gtccttcaca
     1441 caggacctgc gcctggacgt gctcgaacac gcctggcacg gctgatcgtc cttcgggggc
     1501 acgaaaagcg ctgccggcgc gggctgcgct gataccgtgc ccctcatgca ggttcaagcg
     1561 ggcagcggcg cgtccgcacg tactcatgcg caaccctttc cgccagccct caggccggca
     1621 ggaaggcggc gcaccagtcg tgccacagac gtgccttgca cccgtcatcc gtcagcatgt
     1681 cctgcagcga tgggctgagc agcagtttgc gatcatgtgt cacgatctgc gacggtcgtg
     1741 taccggcaca ccataaccag cccgatggat gcgcctgcat gatcggctcc agcgcatccg
     1801 catccagcaa ttgcgcctgc ccgcgcacca gcccgaacgc cagcagcacg ttgtcgagca
     1861 ggcgtgcagc atcgccagcc ggctccccgt cggcgagcag ccagccgcgg tccccgtctg
     1921 caccgacaag ttcgagcaca tgccagacgc gtcgctgcgg ttgattgcgc cgccagcgtg
     1981 gcgccaactc cagcatgtcc agcacctcgt cacgccgcat gttgcagatc cagtaccatc
     2041 aacaaagcat cctcccggcc gttgctggcc ggataatagt ttttgcgcac gccggcacgc
     2101 acaaatccgg ccgagcgata cagggcctgc gccgcagcat tcgactcacg cacctcaagc
     2161 cacatactgg caaaatgcga ttgtctcgaa caggcgacca gacgccgcag cagcgccgcc
     2221 ccatacccca gcccctgacg ttccggcgcg acggtcatat tgagcaggtg cacttcgtca
     2281 accaccgcca tcatcaccga ataggcgaaa atccggccat tttcctcgta cacgcagcag
     2341 cgatgacccg ccagcagcga atcggaaaag ttacccagcg tccagggaaa cgcatagcag
     2401 catcggtcga tcccggcgat gtctgccagg tcagcatcat tcatcggtcg caacatggta
     2461 ttcaacgctc gctgatcttg agcgcaaccc ggtcacgtac atacagcggc agggccagtt
     2521 cgggagccac accgccccgg cccagttcat gctgcgccaa ttcggcaatg cggctggccg
     2581 aaggcagccg ctcgccatca accacctcga cacacccttc aaagcgcgct tccagcgcgt
     2641 ctgcatacac ggaaaacccg ctaccgcagc cacgccagcc tgcaccatca ggcaccggca
     2701 cctccccagg ggcgcagact acggtgggac cgaccgcctg ccaaccgcca tcggcgccac
     2761 gcatccaggc cgcacaatag acttcgccca tgcgcgcatc caggcaggtc agcacgcggt
     2821 cgccatcggc tgaacgtgtc agcgccagca gcgtctcgat acccaccaga ggcagatcgg
     2881 cacccaacgc cagtccctgg gccacggcac aggcaatacg cacccccgtg aacgatcccg
     2941 ggccgcagcc aaaggcaatg ccgtccagcg cctgcaaacg caaccccgct tcgtcgagca
     3001 attgccggca gtgcggcaac aacagactgg aatgcgtctg cccggcatgc acgctgattt
     3061 cgcgcacatg gccatcgatc cacaacgcac tggtgcactg gctggtcgcc gtatcgaacg
     3121 caagtatctt catcgaccca tcggttcgta gatgtcgaac agcacacgct gccacgggtt
     3181 gcggctgcgg gtcaggcgca cgatgcgtgc gcgcagatgg gtgccatggt cgagcaggcg
     3241 cgccacaact gcattgtcgc tgcgcggcac gtagcccagc atgtggccct gccactccac
     3301 gcgcacggca cgtctgtccc acgggttgtc acgatcgcgc accagactca gcacatcacc
     3361 ctcgtgcagc tgcggccaca atgcacgccc ttcgtaataa cggaatccgg ccagacgcga
     3421 agactgcagc cacaattgca cgccggctgc ggcggatgca cccgacgagg cgaccagcag
     3481 cagcaggcac aacccgcacg ctgccaggcg gtcagtaact gatggaacgc cattcgtcat
     3541 ccatgacccg ggaaatatag gccgcaccgc ccgcgatgcc gcaacattcg ggcaccaggt
     3601 cggccgtcgt tacgccatag gcatcgagcg ccccaccgca ggcgaaaaac ctgactccaa
     3661 gttcggccgc ctcctgcatg aaaccataca ccgatttggt gcggttatcg gagggataga
     3721 tctcctcggc aataccgcga cgcaacaggc gcaccgcaga gctggcaaaa tagatttcca
     3781 cctcgacatc catcgccgca gccgcggccg cctgaaagaa cggggtaccg cagaaattcg
     3841 gccgctcagg gccgatcgtc agcagcatga ttaccagttt gtcagccata tgtcctcgtc
     3901 atatcgtatc ggttgccttg tgatccggcg ggcagcctta cggatttcgg gtaaaattgc
     3961 attttatttc actccgccag cattatgcca gatttcctgt ccctgcccgg cagctccgcg
     4021 ctctccccat tccgcctcga caagctgcac gccagcttgc ctgactacct gtgccatggc
     4081 gcgcgcttca tgcattatgt cgaactcacc gctgaagcga cggcaacaga cagagccacg
     4141 ctgcaacgcc tgctcgacta tggcgatcct gcgcccgaac tgacgcaggg tgaggaaata
     4201 ctggtcgtac cgcggctggg cacgatttct ccgtggtcat ccaaagccag cgaaatcgcg
     4261 cggctgtgcg gactgcagca tgtcgcccgc atcgaacgcg gggtctctta ccgtttcagc
     4321 cgccgtgacg gtcgggcctt tagcgccgac gatcgccggc aggtcgcggc gcacatccac
     4381 gaccgcatga ccgagagcgt gctcgacgat accgaccaga cacagcagct gttccagcac
     4441 ctgccagccc ggccgctgga agaaatccca ctgctacagg cggggacgag cgcgctcgaa
     4501 caggccaatc aggccatggg ccttgcgttg tcgacagaag aaaccgccta tctggccgaa
     4561 cagtatgccc gcatgggccg caatcccgtc gacgtcgagc tgatgatgtt cgcccaggcg
     4621 aactccgaac actgccgaca caagattttc aacgccagct gggtgattga cggccagccg
     4681 caggctgaca gcctgttcgg catgatccgc agcacacacg cgcagcggcc gcaggacacg
     4741 atcgtggcct attcggacaa ttcttcggtc atcgccggca ccctggcgca acgctttcac
     4801 cccgacgccg acggtcgtta tgcccggcat gccggtctca cccacatcct gatgaaggtg
     4861 gaaacgcaca accatccgac cgccatctcg ccgtttgccg gtgccgccac cggctcgggt
     4921 ggcgaaatcc gcgacgaagg cgccactggc gtcggctcca aacccaaagc cggactgtgt
     4981 ggcttctcgg tatccaatct gcgcatcccc ggattttccc agccgtggga aagcctgccc
     5041 gggcgaccag agggctatgg ccgtccggca cgcatcgctt caccgctgca ggtcatgatc
     5101 gacggaccgc tgggcgccgc agcgttcaac aatgaattcg gccgccccaa cctggctggc
     5161 tacttccgca cgctcgaaat cgagagtgac ggcgccatgc gtggctacca caaaccggtg
     5221 atgctggccg gcggcgtcgg caacatcgcc gacaatcatt gcttcaagca cacgtttggc
     5281 gacggcaccc tactgatcca gctcggcggt ccggggatgc tgatcgggct cggtggcggc
     5341 gcagcctcca gcatgcacac cggcagcaac gaggagcagc tcgatttcga ctcggtacag
     5401 cgcggcaacc cggaaatgca acgccgcgcc caggaagtca tcgaccggtg ctggcagatg
     5461 ggcgaacaca accccatcct gtcgatccac gatgtcggcg caggcggcgt atccaacgcg
     5521 tttccggaac tcattgacgg cgccgggcgt ggcgcacggc tcgaactgcg caacctgccg
     5581 ctggaagcaa gtggcctctc gccgcgcgag atctggtgca acgaagcgca ggaacgctat
     5641 gtgctggcaa tcgcacctga cgaccttgaa cgctttcgcg cgatctgcga acgcgagcgc
     5701 tgtccgtttg ccgtggtcgg tcaggcgacg caagccgcac acctgcgtgt cgacgaccgg
     5761 ctgttcggcc gcccggcagt ggacatcgat ctggccgtga tcctcggcaa accaccgcgc
     5821 atgacgcgca ccacccggca tcatgtacct gaactgacgc cactgaacct ggacgacatc
     5881 ggcatcaacg atgccgcact ccgcgtgctg cgcatgccct cggttgccag caaggcgttc
     5941 ctgatccaca ttggcgaccg tagcgttggc ggcatgaccg cacgcgacca aatggttggt
     6001 ccctggcaag ttcccgtggc cgatgtggcc gtgaccacgc tcgattacga cggttatgcc
     6061 ggcgaagcat ttgccgtcgg cgaacgtaca ccgcttgcgc tcatcgatcc ggttgcctcg
     6121 ggtcgcatgg cgatcggcga ggcgctgacc aatctggtcg cagccgatac cggcgagatc
     6181 ggtgccgtac gcctgtcggc caactggatg gctgccgccg gtgccccggg cgaggatgcc
     6241 gcgttatacg acacggtgcg ggcggttgcg ctcgaactgt gcccggcact gggcatcagc
     6301 atcccggtcg gcaaggattc gctgtcgatg cgaaccgcct ggcgcgatgg cgccgaggaa
     6361 aaatcggtca ccgcaccgct atcgctcatc atcagcgcat ttgcgccagt ccacgatgcg
     6421 cgccgcacac tgactccgca actgcgtacc gaccagggcg agaccgacct gatcctgatc
     6481 gatcttggcc gcggtcgcag ccggctcggc ggttcggcgc tggcccaggc atggaaccag
     6541 gtgggcgacc gttgccccga cgtggacgac cccggcgtgc tcaaagcgtt ctacaacgcc
     6601 atccgtcacg ccagtgacca gggctggctg ctggcctacc atgaccgttc cgacggtggc
     6661 ctgtgggcca cgctgtgcga aatggctttc gccggccact gcggtgtgac catcgaggct
     6721 gacgaactct gcctcgactg tgtgcgtgcc gaagccggcg acgacgaact catcagcgac
     6781 agcgtcgaag tgcccggcga atgccgccac caactgctgc gcgtgctgtt caacgaagaa
     6841 ctcggcgccg tgctccaggt gaaacgcgaa cacacctcgg cactgatgca ggccttcttc
     6901 agcgccggcc tgcgcagcga actgcacatc atcggcacca tcaacgatgg cggacgcatt
     6961 cacgtgcagc accgtggcga aacggtgctt gacctgacgc gagcgcaact gcaggcgaca
     7021 tggaatgaaa ccagctatcg catccagcgg ttacgcgaca atccggactg cgccgacagc
     7081 gaatttgccg ccagcgcgga tgaaaccgat ccgggtcgct tcatgcagct gaccttcgat
     7141 cccgaacagc acattgtcat cggcggcaac cggccacgca tcgccatcct gcgcgaacag
     7201 ggcgtcaacg gccatgtgga gatggccgct gccttcgacc gtgccgggtt cgatgccatc
     7261 gatgtgcata tgagcgatct gctctccggc cgggtacgac tggacgattt ccgcgctctg
     7321 gcagcctgcg gcggcttttc ctacggcgac gtgctcggcg caggcgaagg ctgggccaag
     7381 tctgtcctgt tcaacccgcg cgcccgcgat cagttctcgg ccttcttcgc ccggccggac
     7441 agttttgctc tcggagtctg caacggctgc cagatgatga gcaacctcgg cgagatcatt
     7501 ccgggcagca cgcactggcc acgcttctcc cgaaaccgct cggaacagtt cgaagctcgg
     7561 ctggtcatgg tggaaatcga cgatagccca tccatcctgt ttgccggcat gagcggcagc
     7621 cagctgccgg tgatcgtctc ccacggcgaa ggccgcgcag acttcagccg caccggcgac
     7681 gccagccgtg tcatccgcgc catgcatttc gtcgatcacc atggccaggc caccgaaacc
     7741 tatcctttca atcccaacgg ttccgcacag ggtctgaccg gcgtcaccag cgcggatggg
     7801 cgtttcacga tcctgatgcc ccatccggaa cgcctgttcc gccgcgtcca gttcagctgg
     7861 caccctgaca gtaccggtga gaacagcgcc tggctgcgca tgttccgcaa cgcacgcacc
     7921 tggatggacg aacaatgagc caacatagcc ccgccccgat gcgccggttc agcgccgagg
     7981 aactggccgc acgcaatgcc gaactcggcg tggacgacac ccgctggtac agcgatgacg
     8041 aactggacga actggagcag cagcagcctc tgctcgcagc acgcattgcg caacggcagg
     8101 caaaaatctc tcgcttgcgc ggctcggttg cgcccggaga ggcgccgccg ctcacccatg
     8161 aagggctgac tgccatcctg gaagaagcgc gggtcgtgct gttgcgcgat ggcggcgacc
     8221 tcgaactggt ggatttcacc gacaccgtgg tgcgggttcg cctcaagggt gcctgtaccg
     8281 gctgccccaa ttcggtactc gatctgaaaa acgtggtgga aacggtggtg cggcgggtgt
     8341 acccgcaggt caccgaagtc cgcaacacgt attgaaggtc tgcattagcg gtcgctgctg
     8401 cctgaccgga gaggcggcgt ctggacggga atggtcgagc tcggcgccgt cacgcccaca
     8461 ccggtttgcg gtaccgacag cggcggatgc gccttgagca gctcctgttt gacgacccgc
     8521 cgcttcgctg gcggcaactt tttcaggcgc aggtagtttc tgcgggcgag atcccgttgc
     8581 tgacgcgtca gtgcgctcca gacggtcagc cgtttgcgaa aacgctgttg cgcctggggg
     8641 ctgagtccgg gatattttgc agcaaccgcc agcaggcgat tgcgttgcac gtcggacatg
     8701 tgaggccaac gcgattgcac cggttgcaga atggtctgct gctcgggcgt gagctgctgc
     8761 cagcctggcg cagccgcaac cgttgtggtt gctgacgccg ccacattgcc agccgccagg
     8821 atcagacacg ccaccagcac acccgctcgt ctgacctgcg ccaacattac gtggcgggta
     8881 gcggccaggt gcctgactag ttggcggaat cgctgctgtc cgcccatttg ctcagatggt
     8941 tgtccagata ggcattgacc ggcagatcgc ccgtcagcag cccggcgtcg atgtccgcca
     9001 tgtcttcttc gttgttgccg gaagactgcc acatgacgcc acccagcacg accagcgtcg
     9061 ccagcaggat accgatcacg ccgacccgat gttgccggat ccagtcaacc agcgtggcgc
     9121 tatgcatccc cctgaaccgg gactcggagg catgctggct cgccagttcg caggcctgtt
     9181 cacgtgcatt gcgcagtctg cgcaaaacat ccggacgcaa atcggccaga ctgtcgtcaa
     9241 gcgcagtcaa tacctttctc gcgtatgcgt cctcattcat cgatgattcc cttgttccgc
     9301 agcagatcgg ccagcgtatg caccgcccgc gagcaatgcg tcttgacgct tccttccgaa
     9361 caccccataa ccgccgcagt ttccgtaatg tccagttctt cccagtaacg cagcaggaag
     9421 gcttcgcgtt gacgcgcggg caatttttct atggcctgct cgatcatggc aatgttcttg
     9481 ccggccgata tgatatgcac cgggtcgtca cgctcgtcgg tggactggac atgttcgagg
     9541 gggtcatcct cttcctggtc ttcatgcccc gacgcaaaga aagacaatgc cgtggtccag
     9601 aaattccgca ctttctggcg ccggtaataa tcacggatgg cattttgcag gatgcgctgg
     9661 aacagcagcg gccattcctg ttgcggcgcg tcggcatatt tttccgccag cttcatcatg
     9721 gcgtcctgca cgatgtcgag cgccgcctgt tcgtcgcgca cggcaaaagc cgcctgacgg
     9781 taggcccggc gttcgacttc ggccagaaaa cggtcgagcg aacccgtggc gctcacgcgc
     9841 ccgcccccca tgccacgccc gacatgatgc ggccactgcc caaccggaat tcgcctgcgg
     9901 cagaaatcaa tgcagcctca tgtccatcca ggacagcgga ttgaaattgc gcgccagcgt
     9961 catgccgaac tgatgggcaa aacggtccgc cagtccgggt tgcggcgtat agtccaccag
    10021 tttttcggca tggatgacat cgcgcgccac gctgtcgaca ctgccaaatc cgtcagccag
    10081 ccccagttgc acgctttgcg caccggtcca gatcagcccg ctgaacatgt ccggagtttc
    10141 cttgagccgg gcaccacgcc cctcgcgtac agcatgaatg aactgctggt ggatctcgtc
    10201 gagcatcgac tgcgcatagg cctgctgcct ggggttcgcc ggcgagaatg gatcgagaaa
    10261 cgccttgttg gcgccggaaa tcagcagccg acggtcgacg ccgagcttct gcatgctgcc
    10321 ggtaaagccg aatccatcca tgatcacgcc gatcgaaccg accagactgg ccttgtccac
    10381 atagatgcgc tcggcggcgg aagccacgta gtatccaccg gaagcgcaaa tgtcatccac
    10441 gaccacatac agtggcacgt gtggatgctt gcggcgcaag cgcatcattt cgtcgtagat
    10501 ccgtccggcc tggaccggac tgccgccagg gctgttgata cgcaggatca cgccggcagt
    10561 gtccttgtcg tcgaatgcat cctgcaatcc ggtgatgatg tcgtcggcat tgacggcatt
    10621 gctggccagc gtgccgctca gttcgacgag cgctgtatgt ctgccaacca tcgacaggtt
    10681 accatcgtcc agccagccag ccagcgacag cagaagcagg gtcaggtaac cgaaaccaag
    10741 cagcttgaaa aaaatacccc agcggcgggc cctgcgctgc tcggtgactg cggcaagcag
    10801 cgttttctcg atcacgtccc gctcccagcg gttgtccggt tcagccaaga ttcatccccc
    10861 gttcattgcc ggctcaggcg tggcgatcga gccagtctgc cagttcggca aacgtgtgca
    10921 ccaggtgcaa cggccggtat tcatgcaatt cttcttcgct gtgcgcgcca tagctgaccg
    10981 ccaccgaagc cacaccggca ttgcgtgcca tcatcaggtc atgactggtg tcgccgatca
    11041 tcagcgtacg ctccggtggc acatcgagca cgtccatcag ttcgtgcagc atggccggat
    11101 gcggcttgga gaaacattcc tccgcgcaac gcgtggcgtg gaaatagtca cgcagaccgc
    11161 tctggtcgag cgcacgatca agcccgttgc gattttttcc tgtggccacc gcgacccagt
    11221 gaccggcatt atggagccgc ggaatcgttt gcgcaacgcc atcgaacagg acgagatcct
    11281 ggtcacgcga cagaaaatgc tgccgataac gggcgatcag cggcggatag tgttcctgcg
    11341 gcaactgcgg ttgcaggcag gcgatcgcct gcgtcagtcc caggccgatg atgtggcgcg
    11401 cctcggactc gcccggcacg ggcaagccga gatcggcgca ggcgtcacgc atcgaacccg
    11461 cgatgacggc cgtggaatcc atgagtgtgc cgtcccagtc gaataccagc aaatcaaaac
    11521 gtttaggcat ggggtttcag tcgatccata aattgttgca attcatccgg cagcggcgct
    11581 tcgatatgca gcggctcgcc ggtcagcggg tgagcaaggc cgatacgcgc ggcatgcagg
    11641 aacatgcgct tgagtccggc ccgcgccagc tcacggttac gcgcaaaatc accgtattta
    11701 tcgtcaccag cgatgggaaa tcccatatgg gcgagatgca cgcggatctg atgggtgcgt
    11761 ccggtgcgca gctccgcatc cagcagggcg aactcacccc agcgcccgac caggcgaaac
    11821 accgtgtgcg cctgtttgcc ctccgggctc accatgacgc ggcgttcgcc ctgggcatcc
    11881 aggtacttgt gcaacggtgc ctgcgcatgg cgcaccggcc agttccagtc gccctgcacc
    11941 aatgccagat aatgcttgtc gatctctccg gcgcgaaact ggtcatgcaa ccggaccagc
    12001 gcactgcgcc ggcaagccag caccagcacc ccggatgtct cccggtcgag cctgtgggcc
    12061 agttcgagga aacggcattc gggcaattcg gcgcgcaact gctcgatcac gccaaagctc
    12121 acgccgctgc cgccatgtac ggccagcccg gccggcttgt ccagcaccag caacgcatcg
    12181 tcgcgccaca ggatacggtc ggcaagccga tggccacgcc ggggcagcac caccggcggt
    12241 ggcgcactcg ccagacgcag cggcggaatc cgcaattcgt cacccaatgc cagcttgcgc
    12301 gcagcgtcga cccgcccttt gttcaagcgc acctgtccgg tacgcaacag gcggtagaca
    12361 tggcttttgg gcacgccctt gagatgcccg aacaggtaat tgtcgacccg ctgtccgtcc
    12421 gcagactcat cgatcttgac aaaagtgaca gattctttgc gtaaatcatt cattttgcat
    12481 atactgtcag cactttttgc gacaactggc cagtccgggc acaacggaaa atttcatcct
    12541 ttgcccgacg cagtgtccag aagtaaaaac aaaccggtta atttcgctca ccggatcccg
    12601 acaggccgca tgcggcccgg gcgcaacccg ctacaggaag aaatcaggaa aatccgacgt
    12661 gcacacatgc tgttgcgcgt ctcttgcccg gattccagtg catgcggcaa tgcccggccc
    12721 catagcgccc tgacaccatc ggacatgaac ggcacattat acacgtcctc atgaccgaac
    12781 acggatcgcg aaccgcatgc atgacagaaa agcggtttgg cgcacaagca gcgatcggta
    12841 gcaacacata tatgaagcgc gcgaagcaac atccagcgag gaacggtgtg agccttacgc
    12901 caacccgcac gggtgaagac acgataatgc ggcctgcaca gcagatcaaa cgctgcgcat
    12961 gcacccggtg aaacggcatg aagccctata catcatgaac cctgattccg aatcttatcc
    13021 tgatcacacc acccgtcggt tcacgcctgc cacagctccg tcctccactg tgtctgcgca
    13081 gcactgcgca gacacgggtt taacctgcca tatcgccatc cttccggtcc tgcaccgcct
    13141 tcacagccat gctgcagtga cagtcgcgac cgtatcgacg gaggtggcct gaacccgtgt
    13201 gaaacgcgcg cgggagaaat caagtcaatg aaacgcatgc tgtttaacgc aacccaggca
    13261 gaagaactgc gggttgccat cgtcgatgga caaaaactca tcgacctcga catcgaatcc
    13321 gcaagcaagg agcaacgcaa gagcaacatc tacaagggta tcgtcacccg catcgagccg
    13381 ggactggaag ccgcattcat cgattacggc aacgaccgcc atggctttct gccgttcaag
    13441 gaagtcgccc gcagctgcta cgccagcggt ggcgattccg gtcgccagcg cattcaggat
    13501 gccctgcgcg
//
LOCUS       DAIANL010000030         4998 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_636619, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000030 DAIANL010000000
VERSION     DAIANL010000030.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636619"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          998 a         1598 c         1471 g          931 t
ORIGIN      
        1 gtttaccatt cgtttttctg acaaggctgc ttatcaacct tgaaaatatg ttagaaggaa
       61 attgtcggca ataggaaccg gccgcaacac cccttggcgt gggcttttac agcgaccggt
      121 ccatccgcgg tttgcgggaa gcacgccgca gcacatgccg ggcgcacact gaaaatcatc
      181 tgcctccccg gcagagacaa ccaaataaaa acgggcagcc gaggctgccc gttgaacttc
      241 cacagcgttc cggcctgcgc cggacgacat cagaaggtgt gcttgatgcc cagcgagaac
      301 gccgagatcg tcgcaccagc aacgcccgta cctgcttcgg ccaccgggct ggtggtgtca
      361 gcaaagttgt aataggcgcc ggcgtcgttg gcgatatggg tgtacagcgc gaacacgctg
      421 gtgttcttgc tgaagttgta atcgccaccc agcgaccact ggtcagcacc cgtgctggcg
      481 ccattggcca gttgcgcgtt gttggcattg cccgcctgag cataggctgc catcacgtcc
      541 cacgcgccca tggtgtgctt gatcgagcca taccaggtgc tgtggcccag caggttggtg
      601 ccgccggtgc cgaagttgtc gctgctgtct tcatacagca gggacagcgt ggtgtccatg
      661 atggtgtagg acgcgcccag cttgtacgca tcgttcgagg tgccacccag cggaccgatg
      721 ccgaggctgc cgttgttagc gccaccgaag tcatgctttt cgtaggcggc ggtcaggtac
      781 agcggaccgg cgttgtacat cgcggcgatc gagtacacat cgccggaagc agacggctgc
      841 gggttggtac caacaggagg ataagagggg ggaacagcgc cggtgttggt ggcatcaccg
      901 aacacatagg cgccaaccac ggtcagacca cccatgttgg gcgacacgta tgccagcacg
      961 ctggacggac gcagatcgaa gtagttgctg gcgctcagac cttggtcagc tggtaaaccg
     1021 acattggtac caacatggta attactggcc atggcggacg aaccgacgat gccgttgtag
     1081 tcacccaggg tgtcggcaaa cgggtccatg ctggcggtgg acattttgta cggggtgtca
     1141 tggataccga ccagcacgct gccggcagcg gtgctcgaca gaccgacgaa gctgttgcgc
     1201 tggttgttga actggttggc gccgaacacg ctgccgccgc cattgaccac gctgccgccg
     1261 ccattgacgt tggtaccctg gctgccgaca accgacacgc cctgctcgat ctgccagatg
     1321 ccggacagac cgccacccag atcttcagaa cctttcaaac cgaaatacga accgtagtcc
     1381 gacacgcgac ccacattgtt ggtgccattg ctggcggtga cgttgctggc ggaatccaca
     1441 ccggcgtcca gcatgccgta cagcgtgacg ttgctgctat cggccagggc gggcatcgat
     1501 acggcactgg cgatagccag ggcgatcagt tttttgttca tgagtccatc tccatagata
     1561 atcttcatct cttgcgcgga agctccacgc ccgaaaatcc ataacttccg acaggaagtg
     1621 aagcagatta tccatttaac agttatcgct aacaacgttt ttcacacaac gttgtctgat
     1681 ttgtagtatt tctgcaacaa ttgtctgcta gccgcgcacc gtcatcaggc gcaaaggctc
     1741 aggcatgact gtgccagcgc acagatatgg atatggacgg gatcagccgg ctacggagtc
     1801 gtcagcactg gtgctgacca gttcgcgcac caggcggaac ccgaggttga catccagttc
     1861 gtcacgcaac cgcggccagc gcgcggcgct gcgcgccatc gccgccggat cgaaccacga
     1921 gccgcctttg gtgacgatgc gttcgttcct gccccgtgcc gggatgtcgt gcccgttgcg
     1981 cggtgtccgt gtgtggtcac tgtgccatgg cgtggctgtc acttcccaca cgttgccatg
     2041 cacatcgtgc agaccccaca ggttcgccgg cttggtgcca acttccattg gccagttcat
     2101 cgaggcacat tgcggcagaa acgaccagag acgtttggtt ttcttgtccg gcaggggaaa
     2161 tatcgggcgg aacagtacct cgcggcaact ggtatcgtca ccgaaatgaa acgccgagtg
     2221 gctgccggcg cgcgcggcat attcccattc ggcctcggtc ggcaggcgat agaactggcc
     2281 ggtctcgcga ttgagccagg caatgtagcg ttcgatgtcg gccatgcgca cgttgacgat
     2341 gggttcgcgc cctttcggcc agatcagttc gcgtccggga cgaaagccgg tggcgcgggc
     2401 gaactgctcc cactgctccg cagtgatggt gaatcgcccg atcgcgtagc agcgttgcag
     2461 ttgcacgcgg aactgtggcg attcgctggc atggtgcccc acttcgtagt ctggcgagcc
     2521 gatctcgaac cagccgggcg gaatcaccgc cagctccggc cccttgctgc cgtcggccag
     2581 cgtatcgctg aaccacagcg atacgcctgc gcgctgcgcg gcctgctgct gtgccgcgcg
     2641 cagcccggcc ggattcaggt catgcatgaa atgcatggcg acacccatgg cacatcaccc
     2701 ctggtaacgg gccacgctcg ccatgatttc agcgcgcgcc tgctcggcat cgacccagtc
     2761 ctcgaccttg acccacttgc ccggttcgag gtccttgtaa tgttcgaaga aatgggcgat
     2821 ctgcgccagc accagcggcg gcagatcctg taccgactgc acggcctggt acagcgggct
     2881 gagcttgtcc accggcaccg ccaccagttt ggcatcgaca cccgattcgt cgctcatttt
     2941 cagcatgccg atggtgcggc agcgcaccac cactcccgga atcagcgcaa acggcgtcac
     3001 caccagcaca tccaccgggt cgccgtcttc ggacagcgtc tgcggaatgt agccgtaatt
     3061 gcatggataa tgcatggcgg tcgacatgaa gcgatcgaca aaaattgcgc cgctttcctt
     3121 gtccacctcg tatttgatcg gcgcaccgtt catggggatt tcgatgacca cgttgaaatc
     3181 gtgcggcagg ttcttgcccg caggcacctg attgagactc atgcttcatc ctcgccaaaa
     3241 aaactgcatt ataacgcaaa ccccgcacgt tcccggcgcc ccggtcatga cggacaggcc
     3301 acagaatact attgtgccga acaggccaat ttacttacaa ttcatgttga caaccgttcc
     3361 acgcctgaaa tagtggcaga ccatcgaata acccatgcgc gcaggcagaa tgaagcataa
     3421 aatggccaaa accggagatt acgcagcgtg gatatcgatc cggttctgaa cgcgaccttg
     3481 gcaagcgcaa ccgtttttcg acagtctgct ggagacacgc aagccatgcg cgtccgctaa
     3541 cggacaaccc gcagccctgc ctgaacctgt tcatgcacaa ccgaaacttt cttttgccgc
     3601 aggcccgcac cgtgctcatg cagtttctgc ttgaccatct ggcacgctgc cagccgggcc
     3661 ttgccgcagc cgcgctgaaa aacgccgacg ccgcgcgcag ttcgctggaa caggattcgt
     3721 ggatcaccct gttcaccctg atcgacaaac gcggcgaaaa cgtcagcgcc ctgctgcgca
     3781 accacctcga acggttgttc gaccgcgcat tgcagaccgc gtacaacacc tggcggccat
     3841 cgtttccgat gggcgcgcga ccgggcgaac tgtcgctgat cggcaacgat gaaatggaag
     3901 acgaactgct ggtcggcacg atcagcgacc gtttccgcaa tgaaatcgcc gacgagttgc
     3961 gcaatctcgg catccgtgtg gcgctgctgt ttggcaccga agaagtccgc gagcgcgaaa
     4021 acccattccg cccctggctc attgcacgtg ccattgccga caccgccacg gaactcgaac
     4081 tccggccgca cgttgccagc cgcttcagtc cgttcgtcgg tgaatgcctg gcgcaatcgc
     4141 tggaacatgg gtatgcggaa gtcaaccgtc tgctggcgca acatggcgtg gcggccgaac
     4201 tggccatgaa gatccgcaaa acaccggatc ggtcagcacg ggcggcagcg acccgcccgg
     4261 atgagcgtgc cgcatcggca gcccacgccg ccacgcccct ggccgatggc cagcccggca
     4321 tgccacagcc ggccgcgcga caggaactgc tcgatgccat cggccgtatc atgcagcaac
     4381 cgggtggcgg cacgccagca ggccgcacaa cgccgatgcc ggacatgccg gcatccagca
     4441 cgctgagcga cgatcccctt gcgcagcgca acctcgacct gttgctcgac tcgatgcgcc
     4501 ggtatgctgc agtatcgggt cacagcgagc gaatggcgaa tgcgacgggc gcggcagcga
     4561 cgcagcctgg ccagtcaccg caatcctccg gggcttcccc accaccgacc tcccggggcc
     4621 gacattcggg ctggctgggc ggcatccagc agatcggcag ccggttgcgt tcgctgctgg
     4681 gcgccagcgc tacggcctac cacggctacg ccgctcccgg ccacgccagc cagccgatgc
     4741 gcacggtcac gccggcgctt gccgcctgca tcgacacgct gcagcagcaa gcgctcgaca
     4801 ccgagcacct gttcgatgcg cgcggcgacc tgcgcaacct gattctcgaa cagcgtccgc
     4861 aattatccgg acacgccgtc cagcaagaag agcagatgac gctcgacgtg gtggcgatgc
     4921 tgttcgaatt catcctgcgc gacccggaag tgccgccgca ggttcgcgtc gaactggcgc
     4981 gtctgcaact gctcatcc
//
LOCUS       DAIANL010000031        10383 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1866601, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000031 DAIANL010000000
VERSION     DAIANL010000031.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866601"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2214 a         3410 c         2807 g         1952 t
ORIGIN      
        1 catatcgcct gcccaaattt atccgtgcta tttactgact cagtgcgctc aacattgtct
       61 gcttccatat tggatatcag cacgttcaat tgatccagac tcagcattat gccctgacct
      121 catcaggaat tcagtcaaat ccaggccatc atcgcaatca tgtggaaatg aatcagcgct
      181 tgcatggcac aaggcatcat acacgccgct ccggacgatc cttcctgcca gccgttactc
      241 agtcggtttc caccgcaatc tcagccgcaa gtacgtggcg ctcgcccggc gcgacagtga
      301 ccgtatcatt tgcagcattg caggtttcca cgcacagcat ctcacgatat tcgtctgttt
      361 tcatatcgga gaacccgcgc gttttttcga tccacgggtt ccacaccacg gtggtgttac
      421 tgcccgactt gtcaatctga atccgccggt cgtgaccaac gtcgtggatc acacagctgg
      481 cgcgggtatc gagatagatg cggtcgacct cgccagcaac cttcacggca ccgtgctggc
      541 gatgacggct catgtcctgc actttatcga agtattccac accgtcaagc ccctcgacct
      601 gggtacgggc aatatcgccg atacgaaaat aggtgtgcag cgcatcagtg atgacaaatg
      661 gttccttgcc ggtgttggtc gtaatcaatt gcatacgcag ggtgcgcccg atcgtcacgc
      721 gcagctcgag atcgaaggca tgattccaga tcgcacgcgt gccttcatcg tcacgcagac
      781 cgagacggac gatgagcaga tcgcccttcc agccagtctc gcgcagttcc cacatgcgcg
      841 tgcgaacgaa accatgcgcc ggcagaccgc cttcacccgc accgaaccac ggccagcaca
      901 ccggaacacc accgcgtacg ccacgcccaa gttcgtagac ggcagcatgg ctcacccaca
      961 gcaccggcgt atgacctgcc ggctgccatt cgagcacctg tgcaccctgc atggaaatgc
     1021 gggcgctggc cagtggcgtg ctgatacgca acaccggaac atcgccatcc ctgacgactt
     1081 ccagcgtgct gatcatgcca aaattttcat tcagcgcggc aaattccttt tcattcatgc
     1141 tcgacttcct ccagttggtg ttcgatgctt tgttcttcct gctgcagggc ccacaagcgg
     1201 gcatatgtgc cctgcctggc cagcagttcg cgatgggtgc ccgattctac cacccggcca
     1261 tgctccatca ccagtatgcg atccgcgtcg acaatggtcg acagacggtg cgcgatggtc
     1321 agggtagtcc ggttgcgtgc gatctccctg agttcggcct gaatgatctt ttcagtttgc
     1381 gtatcgagtg cggaagtggc ctcatcgagt atcagcagca ccggattctt gagcactgtc
     1441 cgcgcgatgg ccacgcgctg tttttcacca ccggacaact tcaggccgcg ttcgcccacc
     1501 aggctgtccc atccgttggg cagcgattcg ataaagtgca ggatatgggc cgcgcgcgcc
     1561 gcctcgatga tttcctcgcg cgttgccgac ggtctgccat acgcgatgtt gtaataaata
     1621 gtgtcgttga acagcacggt atcctgggga acgataccga tcgccgcacg caggctggtt
     1681 tgggtcaccg cacggatgtc ctggccgtcg atgcggatcg agccggacga aacatcgtag
     1741 aaacgataac acaggcgtgc caatgtggat tttcctgcac cgctcgaacc caccacggct
     1801 acgttatgcc cggcaggaat gtcgaaactc acaccgtgca gcaccactcg ccgcgcatcg
     1861 taggcaaact gcacattgtc gaactccagt cgcccaccct gcaactgcaa tggccgtgca
     1921 tccgccgcat cagccacctc gcggctggaa tgcagaatgg aaaacagccg ctcgatgtcg
     1981 gtcagcgact gcttgatttc gcggtacatc actccaagaa aattgagcgg aatgaacatc
     2041 tggatcagaa aagcgttgat catgaccaga tcacccaccg tcagcttgcc cgccaccacg
     2101 ccagccgaag cacgtaaaag catgacggtg accgcaatgg cgatgatgca cgcctgcgcc
     2161 acgttgagca gcccgagcga cacctggttt ttcaccgccg cctgttccca gttacgcaga
     2221 ttggcgtcat aacgctccgc ctcgaatcgt tcgttgctga aatacttgac cgtttcgtaa
     2281 ttgagcaggc tgtcgatggc gcgagtgttg gccagcgaat cgagctcgtt catcttgcgc
     2341 cgcaactggg tgcgccattc ggtgatgatc agcgtcagtg ccacatacac cagcagcgca
     2401 acaagcgtga tggcggcgta cacccagtcg aaacgcacca ggagaatgcc cgccaccatg
     2461 ctgatctcca gcacggtcgg caataccgaa aacagtgtca gctggatcag cgaagaaatt
     2521 ccgcgtgtac cgcgttcgat gtcgcgcgtc acgccgccgg tctggcgctc caggtgaaac
     2581 cgcagcgaca gcgcatgcag gtgattgaac acatcgagcc cgatgcggcg gatggcgcgt
     2641 tgcgtcaccc gtgcaaacac gatgtcgcgc agatcggaaa acatggtcgt ggccagccgc
     2701 agcgcgccat aggccagcag caacccgacc ggaaccatca acgcaggcga cggatggcca
     2761 agccggtcca cgacgtgttt cagcacgatc ggaatcgaca ccaccgccag tttgcctgcc
     2821 accagcaggg acatcgccac gatgacgcgg accctgaact cccacaggta gggaaacaac
     2881 cgggtgaggg tacgccaatc cgccggaccg gtcgggccgg gttcccccct tgaaccatga
     2941 aaaccacgca tgaaatcact caattcaaag tctggtggaa aatctgttcc ggcgcttcca
     3001 gcggcgtggg ctggtacgcc gcctcgtatt ttttcaggat gtcgtaataa gtacggatgc
     3061 tgtcgacaaa aatcaccgcc tctccacccc ggcaatagcc atgcggcgcc gccgcacctg
     3121 caccaccggc aagcaatggc agcgtctgct tgacgtcgca ccacgcttgc ggattcaggt
     3181 gacgccgctc cgccagccga cgcgcatcct cgacatgggc ctgcccctga ttatacgccg
     3241 ccagtgccat ccatatccga tccgatgcat cgatcgacgc gggctgttgc gccagcagct
     3301 cctcaagata acgcgcacca ccgatcacgc tttgctgtgc atcaagccgg tcagagacac
     3361 ccatcttgtc agccgtatcc gaggtcatca tcatcatacc gcgcacaccg gtcggcgacg
     3421 tggccagcgg atcccaatgc gactcctgat aactgattgc cgccagcagg cgccagtcca
     3481 gcccgctggc ctgctgcgcc gactggaaca tcttgcgaaa ccgcggcagc accgtatgca
     3541 tgcgttgcaa gaacccgatc acatcggttt cctccagccg gtcggcataa ccgtaatagc
     3601 ggtcgatcat ctgtttcaga cgcccatcct ggtcgatctg acggaaaaac tgctgcacct
     3661 gcgccaccag caacggactg gcatcacgcg gcaaccacca cgccagcttg ccctctcccg
     3721 gcgccagatc aaacgccacg tccagattgg ggtacagatg gcgcgccagc gcaaacgtcg
     3781 acgaatccac cactaccgcg tccagagcgc cgcgagacaa ctgatcgagc aaactgtcac
     3841 cccactcgct gcgcgactcg atccagcgca atgcgggcac gtgcaggcgc gccgcgcgca
     3901 gcgcatcgac atgccaggta ccagccaccg tcgccactct caaacccagc aacccgctta
     3961 catccgcagg cttgatgccg tcagtgttgt acaccacctg ttgcgttaca ggcaggtagc
     4021 ccggccccag ttgcagcaga aaattgtagt cccgccccgg aatcagactc gcagccacca
     4081 gatgcacctg atgcagtcgc agtcggtcaa gcatacctgc aacgctcgtc tgttcctcga
     4141 agcgcagacg atatccataa tattgcgcaa acaaggtcag aagatcattc tccagccccg
     4201 ccatctgccc gttgacgtct tcataacgcg tcgccggtcc gttacgcgtc acgaccacca
     4261 gttcgccgga acgctcgggc gccgcgatcg gacgctgcac ctcctccggc gcgtgacatc
     4321 ccgccagcca gagcaaccca gcccatatcc atgcgcgtct cagaatcatt tccgcttccg
     4381 gaaagaaaat gcctgcatta tcccctcaac ctggcaattg cggcagcact tgtgcaggct
     4441 tttgctgccc acaatcatga aaacccgccc gagccttcac ctttttggca ggaaacgcta
     4501 taatgcgcgg cttggggaga ggtggcagag tggtcgaatg tacctgactc gaaatcaggc
     4561 gtaggtttat gcctaccgtg ggttcgaatc ccaccctctc cgccaagccg cattccggca
     4621 ccacgcacct cccgacgttc ctgcttttcc ccgctgcatt cacctggttg cctcgcccga
     4681 ttagcccgga ttgaccattg ttcgccgagc cgaataaccg gacaatatcc ctactaaaac
     4741 tttcaatcga ggtgcgcaat gaagctcaag accgcattga tgctagcaac ggtggcgctg
     4801 gcatccgtcg ctggccaggc cgctctggcc tcacctgtgc agcagccgcc tgtctggcag
     4861 atgatcacgc cgaccaccga tccgatcacg atcagctggg ggcagcatga caaccagggc
     4921 tcgatccatt acagccccgc ccctgcggaa acgatctggt actacaatgg caacattcgc
     4981 aaccagagtg taggcaatat cctcaatgtc atcgacacgc aattcaacct ggcttcgggt
     5041 gtactgaaac cggtttcgag ttgcgacagc gccagcggca actgcaccaa tgccagcgat
     5101 ggcagaggcg gcgagggtta cagcaacgga ttcaccagtg acagtgccta caactacctc
     5161 gctgtgcact tcggccaggg tgagctgctg ttccactggg ccaatccgat cgcggccaac
     5221 acggaattca agattgacat cccgtgcggg attggtggca tcagcaacta ccgcgcttat
     5281 gatgcaccgc cccccacccc taccccgatc ccaccggcaa ttctgctgtt tgccagcgca
     5341 ctgatcggcc tggcgctgac caagcaccag cgcatctgat ccgatcggac gttggatgga
     5401 ttcaatcggc ccggcacctg ccgggccgat ttttttgcac aggctctgta tcaccgaacg
     5461 gacaacacga ccgagatggc ctgcacagat cccgtcaccg cagaatcgcg aaactttgag
     5521 cgaaggattg gaacggtccg gccatcagtt gctgcatgaa gtcaccgacc ccaccgtcga
     5581 accgctctcc tccggtctgc gatcgccgag gccgaccgcg cagcgcaagt cgcgcgcatc
     5641 ctggacgatg tcggcagggc gacggcccga aacaccttga gtgacctgtt cacgcgagca
     5701 caagcgtccg gccccattca gggagacacg gtggaaatgg tccgtcggtt tctggcgtta
     5761 ctccgggaag acttgatgct gggtctgttg ctgcgcatcg cggaacggcc aactgacctg
     5821 ccccgaaaca gcaaccgcat caggccacag agcctgttca tgagactcta ccctgatacg
     5881 ggacgagaca tgccgtcccc cgcctgaatc gtgtgggttt ggattccaga acacggtcga
     5941 atagcctttg ggcatccgcc aaccctgctg ggcacatggc ggtaaagttg cgcgcggatc
     6001 cgggctgcga gtgcgcccac gcggtgcgca ccaaaatgaa cgggccgccc acaggcggcc
     6061 cgttcctcat ccagcctgcg ccggatcagt aacgcgagtc cttcggtttg ccgcctttgg
     6121 gccagtcata aatcttggcc ggaaaatcag ggcgcggcat atgagtaaag tactcggcca
     6181 cgtcaatggc atcctggtcg gacagaccac ccgccccaat cgggtaatgc agcgtgttgg
     6241 ccatcggcat gtcattcttg acgaatcctg ccgctgttga ggtcttggca attccggcac
     6301 cgatattgta ggactggtca ccccacagcg gcgggaacac atacgaccca tcggcacgtt
     6361 tcatgccttc gccattctgt ccatgacaca cggcacagga gttgtcgtat acctgcttgc
     6421 catgcaccgg attggccacc agacccggag caaccttgcc cacaccacgt cccggcagct
     6481 tcttctgccc cggtttcaca tcgccgcgca tccagtccat gtaggccagc atggccagca
     6541 tgggcttcga atccttggcc agcggggttc ccgccatgga acgcaggaaa cagccgttga
     6601 tgcgatcggc catgtctata accttgccag cacgggggtt gtagctcggg aacagcgcgg
     6661 ccacaccaac gtagggcgac gctttggcca cggtaccgcc gttgaggtgg cagctgttgc
     6721 aggtcagctg gtcgccgaca ttgttcggca acaggcgttt ggtgtcctgc atcagctgca
     6781 tgccatagat gatctgtgcg gcgttcggac tgttgagcat ggcagcataa cgcggcacga
     6841 cgaaattgct ggccggttca ggcggcagtt cgagcgtctc gcgcatcttg tgcacctgct
     6901 ccgcagtcac ggcatcacca cggttgcccc agcgcgtacg cacaaaggta atgatctccg
     6961 ccagttcctg gtcgttcagg ctggcatagg tcggcatgcc gaatgccttg ggcgcaccgg
     7021 cagtcaccgc cgattttgac ccggtcagca ccacgtgaat gaacgaggac ggatcgttgg
     7081 actggatcga tacgtttcct gcaagtggcg ggaagaaatg ctgcacaccc cggccgtcga
     7141 gttggtgaca ggtcgaacag aactgcacat aacccagccc gccgcgggtg cgatagaggt
     7201 cggcattgct ctcctgctgg ctgatcgccg gggtctgccg gttgatcaca tcttcgggat
     7261 tgggtggcag cgaatgcaga tactcgccga tcgcagccag atcgccttcg gtgaaatgct
     7321 gcgaactgtg gtgaatgact tccgtcatgc tgccaaacgc agtggcattg tcgttttcac
     7381 cggtcttgag gaacgtggcg atctcggcgg gcgtccacag accgcgcaaa ttgaccgcac
     7441 gccagttttc cacattggcg ccggcaagga aatatttgcc gtcatcgccg ctggcgctca
     7501 tggctttttc ctcgaaaccc agtccacgcg gggtatggca cgaaccacag tgaccaaaac
     7561 cttccacgat gtaggcgcct cggttccact gcgggctatg cgaaggatcg tagcggtaag
     7621 gcttgtcatc gaggaacacc atgttccacc cggccaggcc gatacgcaga ttgaagggga
     7681 attgcatggt attcggcttg ttaggctgaa ccaccggtgc cacgccatgc atcatgtatg
     7741 catacagcgc gcgcatgtca tcaggagcaa tcttggcaaa cgacgggtaa ggcatggccg
     7801 gatacaggtt atgaccatcg tcggccacac cacgacgcat ggcgcgatcg aactgttcaa
     7861 acgtgtaatc gccgataccg gttgcgcggt cgggggtgat attggtcgaa tgcacgatgc
     7921 caaacggcgt cttcatttcc agtccgccgg ccatgggctt gccgcccggt gcggtatgac
     7981 aggccacgca atcgcccaga tgggccaggt attcaccatg cctgatcaga tcggcttcgg
     8041 tttgctgttg cgtggcggaa tatgctgttc cggccatcgc gcacgaaaac gcgaaaccca
     8101 ggatcgcccg ctgccaattt ttcactgcca ttcaaatctc ctcgctcatg cagtagtcat
     8161 gggtggcccc gaccattgtc cctgcctgcc catgacggtc atactgcgca ttgtggcttt
     8221 tgtatgtcgc aataacccgt cgattctata ccggaggcga cgcagatatc aaggcaaaac
     8281 cgaacctccg cgctcattca gcgtactgca agaaattcga gcgcatgccc atcgggatcg
     8341 cggcagaaca gcgccgcacg ccctgaactg ctcgggctga agtcaaaacc ggcctgacgc
     8401 aaccgttcgc ccaacgccgc aatgtcgtct acggcaaacg caacatggcg gtcacgcccg
     8461 cccgacaccg tttcctgggc cggcgggcac gaatccggca ggcacaacag atgaatactg
     8521 accgcaccca acgcatacca cacaccgtca aacgtcattg ccggtcgtgc agggtctggc
     8581 agcaagccga gcacaccttc ataaaaccgc cgtgaccgat caagatcagc taccggcagc
     8641 gacacgtgat gaatggcctg aatctgcatc attacgttca tgctccattt tcccggaaaa
     8701 cagactggcc gccatgatca ttgccgcgcc gagccattcc ttgcccacga gccattcatg
     8761 cgccagcaga taggccgaca gcgcagacac caccagttcg gacagcagaa tgacaatggc
     8821 acgattggcg gcgacgtgcg acagtccgta ctgcatgagc aacgtcacgc tgtacatcga
     8881 cagggccacc gccacgagca ccagccaaac ccacggcccg aggccatgca gaccatgcaa
     8941 cgggtgcagc ccaagcagcc cgagccacac cgccggcacc aggctgccgc cggcccagat
     9001 cgccagcgca cgtgcctgca gcaccaattg ctccagccgg cgcgacagta cattggtcag
     9061 cgcgaaaccg ataccggcaa gcagtccgag ccactcggac agattgaccg gcaatggcca
     9121 gcgcccttct ggccgccaca gcatcaccag cgctccaccc agcgacagca acaggatcag
     9181 gtagcccgta accgttaggc gttcgcccag caacaaccgg gcgaacagca ccgtccacag
     9241 tggcgccaga taaaacagca acgccacccg catgacttcg ccgtgaatca ctgccagcac
     9301 ataggccgtg ttggttacgc ctgccaacag cgccagcatg atccacacgc cggtgtgccg
     9361 gaccaggttg cgccagcaac ctgccagcag cggcaggccg agtgcgccgg cgatgatgta
     9421 ggtcagcatg gacgacacca gtccatccat acccgttccc gccagccagc ggtagggata
     9481 ccatacgatg ccccagcaca gtgcgctcat taccagcccg gccaccggca ggaccgtgtc
     9541 atctctgcgc gcccatttca tctcgattcc cccatcacct gcatgccatc tgcctctcgc
     9601 atcccggccg ccgcagggat tataatgccg gtttttacct ggccaggcca tgaacccgca
     9661 tctcgaccgc ctgcaaccct atccgttcta gaaactgcgc gaactgttcc agggcatcat
     9721 accgccacct ggcccgggca tcgacctgtc gattggcgaa ccacggcatc ccgcgccgga
     9781 actcatacgc acgacgatgc tcgagcacct ggaacggttg tcgaactacc cggccaccat
     9841 tggtctcgtc gaattgcgca cggagattgc tgcctggctc ggacgacgcc acgccatccc
     9901 ggttcccgat gccgccagcc aggtgctgcc ggtaaacggc agtcgcgaag cgctgtttgc
     9961 ctttgcccag gccgtcatcg accccacaac cacgccagcc ccggtcgtgc tctcgcccaa
    10021 cccgttttac cagatctacg aaggcgctgc cctgctggca ggtgccgaac cgtggtacct
    10081 gcccacacgg gcggaaaacg gctaccgcat cgacgtgcac gacatccccc aggacgtgct
    10141 gcagcgcacc cagcttgcct atctgtgttc tccgggcaat ccgaccggcc acgtcatgac
    10201 gctggatgaa tggcgcgagt ggttcgacct gtcagaccgc cacggattca tcatcgcttc
    10261 ggacgaatgc tactccgaaa tccatccgga acagtcgcca ccgctaggcg cgctcgcagc
    10321 cgcgcaacag ctggggcgca gtgactatcc gcggcttgtg gttttcaaca gcctgtccaa
    10381 gcg
//
LOCUS       DAIANL010000032         3306 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2886902, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000032 DAIANL010000000
VERSION     DAIANL010000032.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2886902"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          718 a         1051 c          910 g          627 t
ORIGIN      
        1 gccgcggcgc gcgctggcat ccagcctggc gacctgattc tcgcggtggg cgataccccg
       61 gtacacaacg ttgcgcagta tcatgcactg gtcgctgcgg cagcaaaata ccactccatt
      121 gccctgctga tccgccgcga caacgccacg ctgtacgtca ccgtgcagac cgactgaacc
      181 cgctcccggc gaggcagacg cctcgccggg tcaactgtac cccatatgtc cccaccgacc
      241 cctgtccatc tgatcgtcta cctgcgcccc tactgtcacc tgtgccacga cctgatcgaa
      301 cgcctgcgtt gctggcccac gccgcataca ttgcacatcg aaaccataga catcgacagc
      361 gatccgcaac tggaagcgcg ttacaatgaa tgggtgcccg tgctgacgga tgccagccac
      421 aacgaaatct gtcaccactt cctcgatgag gcggctctta ctgcgtattt ggcgaaaatc
      481 cgctagaatg gcgccctgcc cagccagaac gcatcgatga acaacattcg aaacttctcc
      541 atcatcgccc atatcgatca tggcaaatcc acgcttgccg accgcatcat ccaattgtgc
      601 ggcgggctgt ccgaccggga aatggaagcc caggtgctcg attcgatgga cctggaacgt
      661 gaacgcggca tcaccatcaa ggcacaaacc gccgcgttgc agtaccatgc gcgtgacggc
      721 cagacctatc agctcaacct gatcgacacc ccgggacacg tcgacttctc ctatgaagtc
      781 agccgttcgc tgtcggcctg cgaaggcgcg ctgctggtgg tggatgcctc ccagggcgtc
      841 gaggcgcaaa ccgtcgccaa ctgctatacc gccaccgaac tcggtgtcga ggttctgccg
      901 gtgctcaaca agatcgatct gccggcggcc gagccggaac gggtcatcca ggaaatcgaa
      961 gacatcatcg gcatcgaggc caccgatgcg gtacacgcct cggccaagac cgggctgggc
     1021 gtcgccgaca tcctcgaacg tctcatcgag cgcatccctc cccccaaggg tgacccggaa
     1081 gcaccgctca aggcgctcat catcgactcc tggttcgaca attacgtcgg agtggtcatg
     1141 ctggtgcgcg tcatggacgg tacgctgcgt acacgacaaa aaatccgcct catggccact
     1201 ggcgcggtct acctgaccga acaggtcggc gtattcaccc ccaaagccca ggaccgtgac
     1261 cagatttccg ccggccaggt gggctacatc atcgccggca tcaaggagct caaggctgcc
     1321 aaggtcggtg acacggtcac cctcgacgcc cgccccgccg atgctgcgct gccgggattc
     1381 aaagagatca agccacaggt gttcgccggc ctgtatccgg tcgaatcgca cgactacgat
     1441 gcgttgcgtg acgcgctgga gaagctccag ctcaacgacg cctcgctgca atacgagccc
     1501 gaagtctcgc aagcgcttgg ctttggcttc cgctgcggct ttctcggcct gctgcacatg
     1561 gaaatcgtgc aggaacggct cgagcgcgaa tatgacatgg atctcatcac caccgcgccc
     1621 acggtaatct atcaggtggt gctgcgaaac ggcgatgtgg tcgagatcga aaacccgtcg
     1681 cgactgcccg aactgtcaaa aatcgccgag atccgcgaac cgatcatcac cgcaaccatc
     1741 ctggtaccgg aaacctggct gggcaacgtc atcacgctgt gcacgcagaa acgcggcgtc
     1801 cagaccaaca tgcagtacat gggccgccag gtcatgctca gctatgacat gccgatgaac
     1861 gaggtggtca tggacttttt cgaccggctc aaatcggtga cccgcggtta cgcctcgctc
     1921 gattacgaat tcaaggaatt ccgtagcgcc gacctggtca agctcgacat cctggtcaac
     1981 aacgaccggg tcgatgcgct ttcgctcatt gtgcaccgca gcaccagcca gtaccgcgga
     2041 cgggaactgg caagccgcat gcgcgaactg attccgcgac agatgttcga tgtggcgatc
     2101 caggctgcca ttggcgcaca gatcattgca cgggaaaacg tcaaggcact gcgcaagaac
     2161 gtgcttgcca aatgttatgg cggcgacatc acccgcaaac gcaagctgct ggaaaaacag
     2221 aaagcgggca aaaagcgcat gaagcaggtg ggcaacgtgg aaattccgca ggaagccttt
     2281 ctggcgatcc tgcaggtcgg cgacaaataa cccggaaagg ctgaatccat ggattttgca
     2341 ttgatcatgt ttatcgcgct gctcatcacc ggcatgatct ggctggccga tacgctgctg
     2401 ttcaggtcgc gtcgtccggc tgatgcccgc atgccggtgc tggtggaata ctcgcgcagc
     2461 ttcttcccgg tcattctcgc agtattcctg ctgcgctcgt ttctcgtcga accgttcaag
     2521 attccatccg gatcgatgct gcccacgctg cagatcggcg atttcatcct ggtcaacaaa
     2581 ttcacctggg gcatccgtat cccgatcatc aacgtcaagg tgcttgacgt cagttcgccg
     2641 caccatggcg atgtcatggt cttccgctac ccgggcaacc cgagcctcga ctacatcaaa
     2701 cgcgtagtcg gcctgcccgg cgacattgtc agttaccgcg acaaacggct gaccatcaac
     2761 ggcgtagccg tgcccgagca acgcgatggc acctacgatt atgtgagcgg tacgctcaat
     2821 gccgtcaccg cggaacgcta taccgagcgg ctgggcgagc atgtgcattc gatcctgatt
     2881 cagccgacaa cgcctacgct gtatgtcgaa caggttgaca gccagtttcc ctcgcgacag
     2941 aattgcagtt acgatgacaa cggcttcacc tgcaaggtgc cgcccggtaa ctatttcatg
     3001 atgggcgata accgcgacag cagcaacgac agccgttact gggggttcgt gccggatcgc
     3061 aacgtcgtcg gtcgggcatt ccttgtctgg tggaatttcg acaacctgca ccgcgcggga
     3121 catctcatca aatgagtgaa ccgctgcagg gcccggcgaa cgggagacgg aacacgacgc
     3181 atggtgcgca aacattgcaa cacaatttcc tgagggacag ccatatgcat cgatcccagc
     3241 gaggaatcgg cctgatcagc ctgatgctgt ttggtattgc cgcttttttc atcgtcgtca
     3301 tggtcg
//
LOCUS       DAIANL010000033         9198 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1868285, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000033 DAIANL010000000
VERSION     DAIANL010000033.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 9198)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9198)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9198
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868285"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1706 a         2926 c         2701 g         1865 t
ORIGIN      
        1 atccgcacat gctgcgccac gcctttgcct cacatctgct gcaatcctcc ggcgacctgc
       61 gcgcggtaca ggaactgctg ggtcatgccc gcatcagcag cacgcagatc tatacgcgcc
      121 tcgacttcca gcacctcgcg cgcgtatacg atcaggcgca tccgcgtgcg cgcaagaaac
      181 ccgccccctg agccacgcca gcaccctgtt cgcccgaatt tgccacgaaa aaaacgctgg
      241 tccggtttcc cggccagcgt atcgtccgcc ctggacaaac aggtactgct gctcagctct
      301 ccggctgtac cgcaacggta atttcggaaa ccacatcagc atgcaatgca atgctgaccg
      361 gaaactcacc gatgctcttg attggaccac caggcatgcg cacttcattc ttgctcacgg
      421 taatgccttg cgaggtcagc gcatcggcaa tgtcgatatg cgtcacggaa ccaaacaaac
      481 gtccgtccat gcccgccttc tgcgtcagca ccagcgtcag ctcggacagc tggtcggcac
      541 gtgccagcgc ggcagcatgg gctgcagcgt gagccttttc cagctcggca cggcgctcct
      601 cgaactgctt gagattggct tcggtcgcgc gcttggcctt gccttgcgga atcaggaagt
      661 tacgcgcata accgtccttc accttgacta cgtcacccag ggtacccagg ttgacgactt
      721 tttccatcag gatcacttgc atgccgctct cctcaatgca ggtcggtgta aggcatcagc
      781 gcgagaaaac gcgcgcgctt gacggcggtg gacagctggc gctggaaacg ggtcttggtg
      841 ccggtgatgc gcgccgggat gatcttgccg ttttcggcaa tgaaatcctt gagcagctcg
      901 acatccttgt agtcgaccca ggtgatgttc tctaccgtaa agcggcagaa tttgcgacgc
      961 ttgaacagtt ggcgggaatt atcgcggcgc ttggccgtct tgttgttgct ggttggacgt
     1021 ggcatggtgc cctcctgaat tcgtaagcgt tttcatgccg cacactggcg gcgaccgggg
     1081 ttgccgctca ggcagcacct tgtgtttcgg tggccggctt gccttccggc tgcagcagcg
     1141 agcgcgattt ttcttccttc atcatcgggg aaggggctac cacggcctcg tccatgcgca
     1201 cgaccagatg acgcagcacg gcatcgttga acttgaaacc atgttccagt tcgtcgaggg
     1261 tttcctgatc gcactcgatg ttcatgagca cataatgcgc cttgtggatt ttctggatcg
     1321 ggtaggccat ctgccggcga ccccagtctt ccagacggtg aatgttgcca ttgcggctgg
     1381 cgatctgtgc gcggtagcgc tcgatcatcg cgggcacctg ttcgctctgg tcaggatgca
     1441 cgatgaatac aatttcatag tgacgcatat aaactccttg tggatgaagc cccctgcagc
     1501 gtggcgatca cgcctgacgt acagtggagc aaggcgcagc ggtggtacac ccccgaccca
     1561 gggccgaggg aactggcaat tataaatgca cgccggtctt gtggcaagtg tttgcctgta
     1621 ttcgcaattt tgtccgcgcg gattggccgg atgagcaatc tgcagcacat gggttacaca
     1681 ccggaaacaa aaactggcta gaattgaccc tgatgtccgc atcggttttg tgcgccaccc
     1741 acggcactct gagtcaggaa cctgcccatg acagtccgac cgcttcttct gttcgtgccc
     1801 gcgattgtgc tggcgggggg gatcctgttc tggccaccgg cacaccgctg gctgttcgga
     1861 gccccctcaa cacaacagta cgtcacggtc tccggcaaca tcgaagcgca tcaaagcgta
     1921 ctgagctttg ctgccgtaca ggcacccatc attgcgctgc cgttcgacga gggccggttc
     1981 gtacgcgcag ggacagaact ggctcgggtg gacgcgcgtc tgtaccagca gcaactggca
     2041 atggacctgg ccggcgtcgc cgtggcacgg aaacagctgg atgttgatcg cagcaacctg
     2101 ctggccgcag gctataccgt tcgcagcgat cggttcgacc tggtcaacaa acggcgtgat
     2161 gcgcaacgca accaggctca gctcgccatt ggcgcggctt cacaacaagc cagcgacctg
     2221 agcgccaccc aggccgccca ggccgcccag acattggcgc acgatcaggc gctggtagct
     2281 caggcgcgcc ataccctggc gctggcgcaa gccagtctga aatcggccga ggcgcgcgcg
     2341 gctctggacc gggtcaccct gtcctacacc gtactgcgcg ctccattcag cggggtgctc
     2401 agcgtgcggg aagccgaact gggcgagctg gcagggccag gcgtggccat cttcacgctc
     2461 gacgatctgg atcacgtctg gctacgcgcc tacatcaacg aaaccaacct cggccagatc
     2521 cggctcaatg agccggtcga aatccgcacc gacacctggc cggacaagat ctggcacggg
     2581 cgcatcggtt tcattgcctc cgaagccgaa ttcacgccaa agaccgtcga gacgcatgcc
     2641 gagcgcgtca cactggtata ccgcatccgc atcgacatca gcaacccgtc gcaccagctg
     2701 ctgcccggca tgcctgccga agcacgcatt gccctgctgc cggcagcaca atgagctccc
     2761 tgtccaccac aaccgcagct gacgccgtcc cagcggtgcg ggtccatgaa ctgagccggg
     2821 catttggcgc gctgcaggcc attgataatg tcagcttttc cgtggcgccc ggagaaattt
     2881 tcggtctggt cggcccggat ggcgcgggaa aaaccacact gatgcgcatg ctggccggtg
     2941 ttctggctcc ggatcatggc cgcatcgaac tcgacggcat tgatgtgcag caccagtccg
     3001 agcaggccaa gcggcacctg agttacatgc cgcaacgttt tggtctgtat gaagacctga
     3061 cggtggcgga aaacatcttt ttctacggtg aactgttttc ggttgcgacc agcgatcgtc
     3121 gcacgcgcag cgaaacactg ctgcatgccg ccgggctgac cgatttccgc caccgtcgtg
     3181 caggacagct gtccggcggc atgaaacaga agctggggct gatctgcgcg ctgatccata
     3241 cgccgcgcgt attgttgctc gacgaaccga ccacaggtgt cgatccggtg tcacggcgcg
     3301 atttctgggc cattctgtac caactgcgcg aacagggcgt caccatcgtg ctctctaccg
     3361 cctacatgga cgaggccgag cgctgcaccc ggctcgcgct ggtacatggc ggccgcatcc
     3421 gtcaatgtga cacgcccgcc cggctcaagg cggccatgcc gggctcgctg ctggaactgg
     3481 tcgctgccga ccctcgcgcc atccacggcg ccctcaggca tcagcccggc gtgcttggtt
     3541 cgctgctgat gggcgaccgg gtgcatgtgc gcgtggacaa cgccacccgg cgcatgcctc
     3601 agttgaacaa ttttctcggc gagcagggcc tggacatcca gggcatggaa caggtcgagc
     3661 ccggcatcga agatgtattc gtcgccttgc tggacgagga ggcaccggca tgaatgctgg
     3721 cgctccggta atcgttgccg aaggcctgac acgccgcttc ggtgattttg tcgcagtgga
     3781 ccggctcgat ctgaacatcc tgtcgggcga agtggtcgga tttcttggcc ccaacggtgc
     3841 gggcaaatca accaccatcc gcatgctgtg cggcctgttg ctgcccagcg caggctcggc
     3901 ccgggttgcc gggtacgaca tccgcaccca ggccgaagcg gtgcgcgaac acatcggcta
     3961 catgtcgcaa cggttttcgc tctacaacga catgacggta cgggaaaacc tgcgcttctt
     4021 tgccggcatg tatcgtgttg cgcgtagcga gcgtgcgcaa cgcatgaagt tcgccatccg
     4081 catggccggt ctggaagggc gggaaaatgc cctggtgcgc acgctggccg gcggatggaa
     4141 acaacggctg gcgctcggct gtgccattct gcaccggcca ccggttttgt ttctcgacga
     4201 accgacctcc ggagtcgaac ccgaagcgcg gcggcgcttc tgggatctga tccacgacct
     4261 ggccgcccag ggtgtcacca cgctggtatc cacccattac atggacgaag ccgagtactg
     4321 caatcgcatc gcactgatcg atcgtggcag gctggttgcc atgggcagcc cgcgcgaact
     4381 gcgcgcgcac gaactgggcg gcacactgta tgcgctccgc tgccagccac tgggtgcggc
     4441 attgcagctg acgctgaccc agcccggcgt cgtcgaagcc gcgatctttg gcgaccgact
     4501 gcatgtggta ctggcacccc ccggcccaga gtccgcgtcg atcacagccg acgaactgat
     4561 catccggctc aggcaggccg gagtcgacgc cggccaggca cacccggtgt cacccagtct
     4621 cgaagatgtg ttcatccgcc tggtatcgcc accgcaccag aacaggaacc atccatgaac
     4681 ctgcgtcgcc tgtttgccct ggcctggaag gaatcgctgc agatctggcg tgatccccgc
     4741 agcctggcca tcgcactgct catgccactg atgcagatgg gtatcctcgg ctacggtgtc
     4801 agcctggata tcaaacgggt gccgctatgc gttttcgacc aggaaaacag ccagcagagc
     4861 cgcgcaatgg tcaacagttt cgtcgcttcc ggctggtttc gtctgagccg caggctggta
     4921 cgcgacgacg atgtgcgcga cgccatggat cgaggcagct gcagtgcggc gctggtgatc
     4981 ccggtcgatt tttcacgtcg gctgagtcag catggcagcg ctgccgtaca ggcgatcttc
     5041 gatgcgactg acgtcaacac caccaatatt gccctcggct atgcacaggg cgccctggcc
     5101 ctggcatcgc agcagatgta cgcccgctgg gccgcagcgc acggcgtagc cgccacacgc
     5161 gtcggtcagg tcgatcttga gcctggcacc tggttcaacg aaacgctgga cagtcgcaat
     5221 ttcatcattc cgggtgtggt cgccgtcatc ctggcgctgg tcggtgccca gcttacttcg
     5281 ctgaccattt cccgggaatg ggaacggggc accatggaac agctgatctc cacaccggtt
     5341 accgcactcg aggtcatgct tggcaaactg ctgccgtatt tcgccatcgg aatgctggat
     5401 gccgggttct gtctggcgac ggcggtattc tggttccatg tgccgtctcg cggcgacctg
     5461 ttcacgctgc tcatcaccac actgctgttc cttgtcgtgg tactcggcat cggttacctg
     5521 atctcggttc gcatccgcag ccagctgggc gccagccagg tggccttgct gctgaccatg
     5581 ctgccggcaa acctgctgtc aggatatacc tttcccatcg atcagatgcc gggcgcgatc
     5641 cagacgttgt cactggtggt atatgcccgt tactacgtca gcatcctgcg cggcgtattc
     5701 ctcaagggca gcaccattga cgacctgtgg tttccgatcg ccgggctgtc cctgtacgcg
     5761 ctgctgattc tctggctggc cgcgcgcgct tttcgcaagc ggctggactg aaccgtgttc
     5821 gaacgtctgt atgtgatgct gatgaaagag ttcatcgaac tcaaacgcga ccattgggcg
     5881 ctgtttcgcc tcacggtacc gctgctgatc cagatgctgg tattcggtta tgcggccacg
     5941 tttaccgtac accacgtgcg cactgcggtg ctcgacctgg accacagcca ggccagccgc
     6001 gcactggttt cgcattttgc cgccagcccg aatttcgcca tagtcgacca gcccgacaca
     6061 cccgcgtcga tcacgcgcga catcgaccag aacaaagcgg tcatcgctct ggtcatccat
     6121 gccggattcg gccggaaact cggcaatggc acccgtgcgc cactgcaggt catcgtcgac
     6181 ggcaccaatt ccaataccgc cctgattgcc ctgggttatg tcaaccagat cgtggcgcag
     6241 ttttccgtcg cacagccgca gtctccggcc atgatcgccc ccacggtgac tcctgccgcg
     6301 ttcgtgacgt tgcaggaacg tccctggtac aacgaagggc tcgatgaacg ctggtttttc
     6361 ataccggggg tgattggcac gctgacgctg atgcaggttg tcagtctgac cgccttcgcc
     6421 atcgtgcgcg aacgggaagt cggtacgctg gaacagatca tggtcagccc gatacgtccg
     6481 ttcgaattca ttctgggaaa gaccatgcca ttttttctca tcgggctcgc agacatcggt
     6541 ctggtcgtca gtttcggcct gctctggttt catgtaccgt ttgtcggcaa cccctggatc
     6601 ctgctgctgg gggcttcact gttcctgttt gccgcactgg gcatgggatt gttgttgtcg
     6661 acgttttcgc agacccagca acaggctttt gcgttgaatt tcttcttcgt caatccgttc
     6721 ttcatcctgt ccggattcgc gtttcccatc gcctccatgc ccaaagcact gcaatggttt
     6781 actgtaatca acccgttgcg ctactttctg gtaatcatcc gtgccgtatt tctcaagggg
     6841 gtgggactgg aggtgttgtg gccgcagttg ctggccctgg ccgtactggc gctgggcatg
     6901 ctcacggcga gcaccctgcg gttcaggaaa tcgctcgact gatctgcgac acgggtacca
     6961 ttacaggttc agaacacgac aaacgacagc gccgccaccg ttgcgcccag cagcgcaccg
     7021 gccagcacgt cggtcgggta atgcagcccg agcaccagcc gcgacgcggc taccgccatc
     7081 gtgaacggca cgagcaacca ggccagcaga gggtagtaat acaccgccac cagcgtgaag
     7141 gtaacggcat gcagggtgtg gccggaagga aagctgaaac ggtcgagcgg tgcagtcagg
     7201 cagtagatat tgccaaactg ctggtgcggt cgaggacgca gcgtcttgtg cttgagccat
     7261 ttgtagatca gcgtggcgct gagtcctgct gcaagcatct gcagcaccac cggtaccgcc
     7321 gtcgcgccct ggaacaggta catggcaatc atcagcgtat accagaacac gccatcaccc
     7381 agccggctga tgcggcggaa cgccaccagc accacaggat agcgcccgac ccggttgaat
     7441 cgccgggtca ggttcgcttc atattcggcc atccactgcc agctcagctt ctccacaggc
     7501 gtcttccttg tcgatgatac tgtgcaggat gcgttcgaac cggtcgtgca cgctttccca
     7561 ggagatggac atcgccgttt cgcgagagtt cagccggatc tgctttatca gttccggatt
     7621 gactgccagt tcggtcgcag ccccgatgaa agcggaaaag tcgttgcacg ccgccagcat
     7681 gccattttcg ccatgacgaa tcaatgcctc ggccgcagca taattgtagg ccaccaccgc
     7741 cagcccgctg gccattgcct cggtggtgac attgccaaag gtttccgtca ggcttggaaa
     7801 taaaaaaaca tcgccggaag cgtaatgccg ggccagttcc tcgccactgc gcacaccgca
     7861 gaataccgct tccggatact gttgttgcag tgctgcgcgc tcgggcccgt caccgaccag
     7921 caccagccgg ctatccggat tggctgcgcg catggcggca aacgccgtga acaccagcga
     7981 caggtttttt tccggcgcaa tccgccccac atacatcacc acccgcgtcg aggcgcgcgc
     8041 gccccagaac gagcgcagct ccgcgtctcg ccgtccggga tgaaacagct cggtgtcgac
     8101 accacgtgac accacctcca gattgcggta gccgtgttcc tgcaactctc ggcgcatgct
     8161 ttcggtcggc accagcgtca cgcacgcctt gttgtgaaac ttgcgcaagt aggcgaggat
     8221 gggacggcgc agccagccgg cgccgtaatg tttgctgtag ctgtggaaat tggtgtgaaa
     8281 atcggtgctg accggcaggc gcagtttctg cgccgcggcc aatgcggacc agcccagtgg
     8341 tccttcggtc gcgatgtgga ccaggtccgg acgccgcagc tgccattgcc gtaccagcgc
     8401 ggtcttggca ggcaacccca gcttgagtcc ggcatagcgc ggaattggca cgccaatctt
     8461 gagcacctcc tcgagctgct cgctgcgcgt ggcgcgatcg gccgcactct gccgcgggcg
     8521 gatcaattgc acctggtgtc cacgcgccag cagatcgctg acctgacgcc cgagcgtcat
     8581 cgccacgccg ttgacttcgg gaggataggt ctccgtcacc agcgcgatac gtaacggctg
     8641 cggccgcgga gcaaatatct gcaagaggaa ttcatcactc atggcggcat tgtgcagaga
     8701 acgcgctgca atttcgtgaa tccttcgtga agctttggtg acggtttgtt acacccggct
     8761 gacacaatcc tccacatccg tgactgctgt cgcgtcagcg aacacttcct gcccaccctc
     8821 gctgtttgct acactgcccg catgtgtgta tggatcgccc gcgttgcact cgatattccc
     8881 gtattccgcc tgttcgatta tctggcagga gacctcggga tacaggacat cggctgccgg
     8941 ttgcgggtgc cgtttggctc gcgcacgcgc gtgggcatcc ttgtcgcgct cacggatacg
     9001 agtgattggc cagccgacca gctccgccgg gtcgtcgaaa aactcgacga gcagccgctg
     9061 ctgagcgcag aaatcctgtc gctgctcgat ttttgcgcaa aatactacca ttacccaccc
     9121 ggtccgacgc tgttctctgc gctgcctggc acgctgcgcc gggccgtcac gccacgcgaa
     9181 ccggcgcaca catggata
//
LOCUS       DAIANL010000034        27084 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1869346, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000034 DAIANL010000000
VERSION     DAIANL010000034.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 27084)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27084)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27084
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869346"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5502 a         9139 c         7569 g         4874 t
ORIGIN      
        1 tcgacctcgc cccttacacc ccgggaaaag ttgcccggtt caccctgcac tgatgccttt
       61 tgccatgttc gacatccaga ttccccgtcg ccctacccgc aaggtcatga tcggaaacat
      121 cgccgtcggc ggtggggccc cgatcgtggt ccagtcgatg accaacaccg acaccgctga
      181 catacagtcg accgtcgagc aggtgcatgc gctgtggcag gccgggtcgg aactcgtacg
      241 catcaccgtc aacaccatcg aagccgcgcg tgcagtaccc tacatccgtg atgcgcttgc
      301 gcagcgcggc tgcaacgtcc cgctcatcgg cgatttccat ttcaacggcc acaagctgct
      361 gaccgccgaa cccgcctgtg ccgaagcgct ggcgaaatac cgcatcaatc ccggcaacgt
      421 cggacgtggc gcaaaacgtg acgagcagtt cgccacgctc atcgaaaccg cctgtcgtca
      481 tgacaagccc atccgcgtcg gcgtcaactg gggcagcctc gaccaggcac acctggcacg
      541 cctgatggac gaaaatgccc accgcaccca gccacacgag gcggaatggg tcatgcgcga
      601 agcactgatc acttccgcac tcgaatccgc acacaaggcc gaagaaatcg gactgcctgc
      661 cgaccgcatc atcctgtcct gcaagctgtc gagcgtgcag gatctgatca gcacctacca
      721 ggagctgtcg tcgcgcaccg actacccgct gcatctcggt cttacagagg ccggcatggg
      781 atcgaaaggc atcgtcgcct cgactgccgc actggcggtg ctgctgcagc agggcattgg
      841 cgacaccatc cgcatttcac tcacccccga acctggcggt gaccgcacac gtgaagtcat
      901 cgtcgcacag gaaatcctgc agaccatggg gatgcgctcg ttcacgccgc tcgttgtcgc
      961 ctgcccaggc tgcggccgta ccaccagcac cgtgttccag caactggcac aggatatcca
     1021 gcgccatctg cgcgagcaga tgcccgtctg gcgcagccgc catcccggcg tggaaacgat
     1081 gcaggtggcc gtcatgggct gcgtggtcaa tggccccggc gaatcgcgcc acgccaacat
     1141 cggcatcagc ctgccaggca ccggagaggt gccggtcgcg ccggtctacg tcgatggcga
     1201 aaaaacggtc acgctcaagg gcgaccgcat cgccgaagaa ttccaggcca tcgtcgaaca
     1261 gtatgtcctg gaccattaca cggcatcggc agacatgacc gaagcaaaaa agatcattcc
     1321 cctgcatctg caaccatgaa cgcaccgatt caagccatcc gcgggatgaa cgatatcctg
     1381 cccgaagaaa cgccacgctg gcagcacctg gaacaactga cccgcgactg gctgtccgca
     1441 tggggctacc acgagatccg taccccgatc atcgaacaga ccggactgtt caaacgcgcc
     1501 atcggcgaag tgacagacat cgtggaaaag gaaatgtatt ccttcaccga tgcactcaac
     1561 ggcgaacaac tgtcgctgcg tcctgaaggc accgcagctt gcgtgcgtgc ggtcgtgcaa
     1621 cataatctgc tgtaccagtc ggcgcagcgt ctgtggtatt ccggaccgat gttccgtcac
     1681 gagcgcccgc aaaagggtcg ctaccgccag tttcaccaga tcggtgtgga agcgatgggt
     1741 tatgcagacg ccgacatgga tgccgagcac atcgtcatga cggccgatct gtggcgtcgc
     1801 atcgggttgg gcggcatccg cctcgaactc aatacgctgg gcgacagcga cgctcgtgcc
     1861 cgattccgca atcgcctcat cgactggttc gaaagccatg ccgaccagct cgacgatgat
     1921 gcccgtcgcc gcctgcacac caacccgctg cgcattctcg acagtaaaaa ccctgccatg
     1981 caggccctga tcgaagccac gccgcgtctg gaacatgacc tggacgacgc cgctcgcgct
     2041 catttcgaga ccgtgcagcg catcgtgcgc gcgcatgaca tcgagttccg cctcaaccac
     2101 cggctggtac gcggactcga ttactataac tacaccgtgt ttgaatggat caccgacgaa
     2161 ctcggtgcgc agggcaccgt gtgcgccggc ggccgctatg acggcctgat cggccagatc
     2221 ggcggcaaac ctcaccctgc ctgcggctat gccatcggca tcgagcggct gctcgcgctg
     2281 atggaacatg cgggcatcgt acctgtcgat gcgggacctg atgcctacgt cgtctttgcc
     2341 ggtacgcatg ccgaagcctt tgcctggcaa agtgcgcgca gcctgcgcga agccggtctg
     2401 cgcgtcgtgc tgcactgcgg tggcggcagt ttcaagtcgc aaatgaaaaa agccgacgcc
     2461 agcggcgccc gttttgcctg catcatcggc gacgacgagg ctgccagcgg ccagatcagt
     2521 ctcaagccgt tgcgcgatgc ccgcgagcag gcccgcatgc cggttgaaca cgtcatctcc
     2581 acccttttga gcaaggattg cgcatgagca gcgaactgac cctggacgaa caggaaaaaa
     2641 tcgaccagat caaggacttc tggaaacgct acgccaatct ggtcctcggc gtgctgctca
     2701 tcctcgccat tgccgcgacc ggctatacgt ggtggcaccg ccagaccctg cacaaatcgg
     2761 ccgaggccgc cagcatctat gccacgctgc aagcgatgca gcaagccggc cagaccagcg
     2821 cggtcagttc gctggccgac acactgatga agcagtatcc cgacagcgcc tacagcgcac
     2881 gcggcgcgct catcgctgcg gccaccaatg cggcagcgca caatgacgcc gccgcccgct
     2941 ccgaactgca atgggtactc gaccacagca gcgaatccgg catgcagacc atcgcccggc
     3001 tgcatctggc cggcatgttg ctcgatgcgc accagcccgc cgaggcagta aagatgcttg
     3061 accgcccaca tgacgagagc gatgccgatc tctacagcga tctcaagggc gatgccctgc
     3121 tcatgctggg ccggcgcgac caggcccgcg tcgcctacca gacggcgcta cagaaactgc
     3181 ctgccgacag tccataccgg caaatcgtgg aaatcaaact caatgccatc gccggagctg
     3241 cccagccatg aaccaccgaa tcacccttcc gctggcgctg ctggccactc tgacgctcgg
     3301 cggttgcgcc catttcgtgc acaacctgaa cccggtcaac tggttcggag atgacaatcc
     3361 tccgcctcca ccggcaccgc tcgtcgcgct caagccggca atccaggtcg gcatgtcatg
     3421 gcatacccgt ctggatggca agtcacccaa cacgttcagc ccggctggcg tgctcgatgc
     3481 gattttcgtc gccgacaaca agaatcaact gctgcgcctc aatccggaca acggcaacac
     3541 cgtctggcgg accgccctcg gcatgtccga cacagcggcc gtcggcgcat cgcccgacag
     3601 cgaatacacc ggatcgctca aaggtgaact caaagcctgg aatcgtgacg gcaaactgcg
     3661 ctggagctcg cagttgtcca gcacagtggt cggcgtaccc aaggaatccg acggggtggt
     3721 cgtggtgcga accgaaaatg gtgacattta cggactggat gacagcaacg gcaagcaaaa
     3781 atggcattac cagcacaccc tgcccccgct cacgctgcat gcctcaccca gtgtgaccat
     3841 ttatcgcagc ggcgtgtttg ccggctatgc cggcggcaag ctggtcgcgc tgacactcga
     3901 cactggcacg ctgggatggg aagccgatgt ctcggtaccc cgcggcgcca gcgaaatcga
     3961 acgcgtcaac gacgtgacca gcacgccggt ggtcgatgac gaggaagcct gcgcagtgtc
     4021 ctatcagggc aaactgtcct gcttcagcgt ccgcagcggc gcgctgatgt ggtccaagga
     4081 gatgtccagc actgcgggac tggccatgga cagcgacaat gtctatgtca gtgatgttga
     4141 aggcaacatc tacgcctatg aaaaggaaca cggcgtcaac cgctggaaac aacccgcatt
     4201 gcatctgcgc caactgaccg caccgcgtct catcggcggc tatcttgcgg taggtgacta
     4261 tcagggctat gtgcacttcc tttccctgtc cgacgggcac ttcgtcgcgc gcatcgccac
     4321 cgatggctcg cccatcgtcg tacaacccat ggaataccgc aacaacctgc tggtgcaaac
     4381 cagcaagggt ggcgtgttcc tgatcaagat gccgtaacgc gcccatgaaa cctaccgtcg
     4441 tgctggtcgg ccgtcccaat gtcggcaaat ccaccctgtt caacaaactc accaaaaccc
     4501 gggacgcgct ggtcgccgac cagcccgggc tgacccgcga ccggcactac ggccagggcc
     4561 gcattggccg caaaccctac ctgatcgtcg ataccggcgg cctggaaccg gtggtcaagg
     4621 aaggcatcat gcacgagatg gcgcgccaga ccctgcaggc catcgacgaa gccgacgtgg
     4681 tgctgtttct ggtcgacagc cgcaccggtt gcaccgcgca ggacaagatc atcgccgacc
     4741 atctgcgccg cagccattgc ccggtgcatc tggtggtcaa caaggcggaa ggcatggcac
     4801 gcgaggtggc cggcgccgaa ttccatgaac tggcgctggg cgaacctgtc gccatctccg
     4861 ccagccacaa cgaagggctc aatgagctca tggacatcgc gctcgcccat ataccggacg
     4921 cgcccgaaga agacgacagc cgccatccga aaatcgccct gatcggccgc ccgaatgtcg
     4981 gcaaatcaac gctggtgaac agcctggtcg gcgaagagcg cgtgatcgcc ttcgaccagc
     5041 caggcaccac ccgtgacagc atctacatcg atttcgagcg caatggccag acctggacgc
     5101 tgatcgacac cgccggtgtg cgccgacgcg gcaaggtcac cgaaacggtg gaaaaatttt
     5161 ccgtcatcaa aaccatgcag gccgttgccg acgccaatgt cgtcatcctt gtgctcgatg
     5221 cccgtgccga catttccgaa caggacgcgc acctcgccgg tttcgtgctc gaatccggcc
     5281 gcgcgctggt cgtcgccatc aacaaatggg acggactgcc gcaggaacag cgcgacaata
     5341 tcaaacgcga gatcgaccgc aagctcaaat tcctcgaatt cgcgcgcttc cattacattt
     5401 ccgcattgcg tggcaccgga cttgatacgt tgatgcgctc ggtcgacgaa gcatggcatg
     5461 ccgccatgat caaactctcg acgccgcaac ttacacgggt actgatcgac gccgtcgccg
     5521 cccaccagcc accgaagacc gggccattcc gtcccaaact gcgctacgcg caccagggag
     5581 gcatgaaccc gccgctgatc gtgatccatg gcaatgcgct cgacaccatc ggcgacagct
     5641 accgccgcta cctggaaggc gtgttcgtgc gcgcattccg cctgcacggg acgccactgc
     5701 gcatccagtt caacgtcagc accaatccct acgcagaaag aaagacggcc acacccaccg
     5761 tttcggccgc ccgcgcctca cagcgcgtcc ggcccgcgtt ggcggcaaaa ccgccacgat
     5821 cgcgcaaacg cacggcagat taacgctccc aggcgaacat gccgtggcgc gactcccccc
     5881 gcaaacaaca cattgcgggc caggccgcgc agtcactcca cccgttcata catctgcagg
     5941 tacaccgact tctccagcgg ttcgagcctg gccaccgcca gcatgtcctg caggtgcgcc
     6001 ggtgagctca tgccgatcag cgatgtgccc agccccggcg tggagcggtt gaactgcatg
     6061 gctcgctgtg cgggattcgg cagactggcc agcgtttgca tcaccgcatc cagcgacgtc
     6121 tgtgccagac gccccttgaa cagaccgtga ctcgccatca cgtacaatcc cagctggtag
     6181 gccgcctgca gcgatgacgc cacattgccc tgtccggtcg cctgattgaa acgggtgaac
     6241 ccctcctgca tcacctggtt gaacggcatc tgcaccacac ggaaattcgg cttcgcccgc
     6301 gcgtcctgcc atacggcctg ggcagccttt tccgccagtc ccagcatcga tgtgaacgac
     6361 tggaacagca cattgtcggt ttccacgcgg aaaccgttga atgtggaaat accgtaataa
     6421 cgcaggcgcc cctcgcgcac agcctgttcg agcacccgga acacggcttc cagcttgcgg
     6481 ttgagcattt ccttgccgat gaccggaata tgcacctcgg gctgatcgat cagaaaggca
     6541 tccagggtct ccactcccat cagacggcgt gacagttcga gctggaagcc aatgtattcc
     6601 gggctgatgc aatgcccctg caccagatca tcctttctgc ccagtccggt cagcacgatt
     6661 tcacgctcga accatgcatt caggtcatcc ggacggccgt cgcgaaagcc gagaaacccg
     6721 cctttcgaca ccagaaacat ctgctcgcgt ttcacgcctt gcgccagcgc agcacggata
     6781 ccttcaccca gcgccgagca cgaacgcccg tagcggtagt gcgcaccggt atccactacg
     6841 ttgatgccgt tgagcagtgc atcgcgaaca atgctggcga tctgcgcatc cacctcatcg
     6901 gtgggcgccc ccggaaaact gcccacccca agcgaagaca gacgcagacg ggtctcggga
     6961 aaatcgctga agtgcccacg accgcaggct ttgccaaaag tggaaacata ttctcgggtc
     7021 gcctctgacg tggcgtatcc cgcaacaagg ggagtactca tggttgcatc cggaagtaag
     7081 ggtgatcggt cagtctggcg gacagacgtt cgacttccgg gcgaatgccc gggtcggtca
     7141 tgcggtcgac acgcgattca aacaacgccg cgaaatatgg ttcgtcctcg tcggcatccc
     7201 aacctgccgc gcgcattgca tcggcacccg ccggcggaat ggcttcgggc aggctgcgtc
     7261 ccgacatcag cgcccacaca cccgcccagg cacgcgccag caccagcgga tgatacccgc
     7321 cgccaccggt taccagcaag cgcggcgtgc catcggcgtg acgcggcgca cttgtcagca
     7381 cacgctgcac cacagcgagg aatccctcgg tcgacattgc cagtttacca agcggatcgg
     7441 cgtggatcat gtcggtaccc gcctgcaaca ccaccacgtc cggtcggaac cgatcgagca
     7501 acggctgcca cacgctgtcg aacagcagat gatattccgt atcgccggtt cccgctggca
     7561 acggcacatt catgctggtg gctcccgccc tggcagtatc gtccagcccg ccgccggaaa
     7621 acggataggc gtagccggtg tccatgtgca gcgaaagcgt gaacacctga tcatcgtcgc
     7681 tgaacgcagc ttcgacgccg tcgccgtggt gagcatcgat atccacatag agcacacgcc
     7741 agccggaacg tcgtagccgc acaatggcga gcacgaggtc attgaaataa caaaatccct
     7801 gcgcatggtc cggccgggcg tggtgcatgc cgccggcagg attgaacgcc atccggcccg
     7861 ccagcacgat ttcggcggcc tgaatggatg cgcccgccgc cgtggcggga atggagaaga
     7921 aatgttcaaa atacgggttc tccagcgtac cgagccgatg ccgctcgcgc accgcctggc
     7981 tgacgcgccc cactgcctcg caacggcgga acgccgatac gtattccggc gtatggaacc
     8041 actgcagttc gaattcggcc gcgcgtcgtg catgcacaaa ctcggttgca tccagcgcgc
     8101 cataggcctc gatcagttgc gtggccagcg atacgcgcgg aatggccagc ggatggttgc
     8161 tgccgtaggc acggcggcga tacgccgatc cgcccacata caccgcccgg cccggcagtt
     8221 gccgatcctc cccggtcatg tcatgcctgc cttttttcga gcaggcagac ggcctcggct
     8281 gcaataccct caccgcgtcc ggtgaacccg agcttctcgg ttgtggtggc tttcacattc
     8341 accaccccgg ccgccacgcg gagatcagcc gcgatgcgtt cgcgcatcag cggaatatga
     8401 cccgccatgc gcggcgcctg ggcgatgatg gtacagtcca cattcaccac ccgccagccc
     8461 tgctgcgcca gcaaggtttc cacctcgcgc agcaggcgac ggctgtcgat gttgtggaaa
     8521 cgcgcatcgg aatcgggaaa atgcctgcca atatccccca gtccggcagc gccgagcagc
     8581 gcatcgcaga tcgcgtgcaa cagcacgtcg gcatccgaat ggcctgcaag gcccagttcc
     8641 cattcgatct ccaccccgcc tatgaccagc ctgcgctgcg gcgcaaaggc atgcacatcg
     8701 aaaccgtggc ctatcctcaa cgtcgtcatg ttccttcctt cgatttcccg gcagcagcca
     8761 gccgcaaccg cgcaagcacc agatcgtctg gccaggtcac cttgaaattg cccgcacgcg
     8821 attcgaccag caacggcgac agcccaagcg cctccaccgc gctcgcctcg tcggtcatcc
     8881 gggcgccgtc ccgggctttc agcgcgcgca gcagcaagcc gctgcggaac atctgcggcg
     8941 tctgcgccag ccacaatccc tcgcgcggca ccgttgcact cacgcgctgc ccgctgccga
     9001 tgcgcttgag tgtatcggcc accggaatgg caagcagacc gcctactgca tccgcaccca
     9061 ccgtatcgat caaacgatcg atgaggtctt gctccagaca gggacgtgcc gcatcatgca
     9121 ccagcaccca ggcatcgtcg ggcaactgca tcgccgtcag cccgttggtc acgctctgtg
     9181 cacgcgtatc gccgccgcag cgcaacacct gcaaccggcc ggaggcgatg tcatgcgccc
     9241 actcggcctc agaccaccag atatcgcctg gcgccagcac caccgtgacc cgatccagcc
     9301 tgctgcatgc cagcaacgcc tgcacgctcc acgcaatcat ggatttgcca gcgatctcca
     9361 gatactgctt cggctgtgcc gaccccatgc gcgtgcccga tcctgccgcc ggcaccaggg
     9421 cgtaataacc tgttccatcc actctgtctg cttccagcgc catcattccg tcccgcaacg
     9481 tctgcagcat tgccagacgc gaaagggaat cattctacac agttgaggcc gcatcttcca
     9541 gacgaccccg ccgcaaaaaa catcacccgc cagaccacgg cgggccatcc ggagaatggc
     9601 cgcatcccgc catggacacc ggcgcgaatg cgccacgcgc gcgcctcctg gcttgacaaa
     9661 tcggcgtaaa accggataat cgtcagtctt ctgcagcgcg ctgccgcaat acgcgctctg
     9721 tgctgcggcg gccacctgtg gcatcccggt taccggatgc cgcggtgcag gacgaccgac
     9781 tcacggaatg tagcccagcc cggttaaggc gccacgttcg ggacgtggag atcgaaggtt
     9841 caaatccttc cattccgacc agatattcgc cgtccgcatc ctgtcccgcc agtcgcggac
     9901 ggcacaccac cacaacacat ctgcctcgcc attgcagtcc gatcggacta taatcgcaac
     9961 accatcgatc cgccgtcatc cacccatgca attcgatccc agtctgctgg caaaatacag
    10021 caagcccggc ccgcgctaca cgtcgtatcc taccgcgccg catttttccg aaagcttcgg
    10081 cgaacagcag tggctggaag aactgcgcgc cacgcgcgat tgcgaacgcg acctgtcgct
    10141 gtacacgcac atcccgtttt gcgacacgct gtgctactac tgcggctgca acatggtcgc
    10201 cacccgcaac tacaaccgcg ccaccgaata cctcggccac ctgttcaacg agatcgaccg
    10261 catggcggcc ctggccgctc caggccggct ggtgcgacag ttgcactggg gcgggggcac
    10321 accgacctat ctgcatcccg acgacatccg ccgcctgtat gcccatatcg cctcgcgcta
    10381 tacgatcgcc gcagacgccg aaatcggctg cgagatggat ccgcgcgaac tgactcgcga
    10441 gcatgtgctg gcactgagcg aaagcggttt caaccggctg agccttggtg tacaggaact
    10501 cgacccgctg gtacaggaag ccgtcaaccg ggtgcagccc gaagcgatga tccgcgaagt
    10561 gtacggctgg atgcgcgaca gcggcgtggc cagcatcaac atggacctga tggtggggtt
    10621 gccgcgccag agcattgccg gtttctcgcg cacgctggac cgggtgatcg acatggcgcc
    10681 ggaccggctg gcagtgttca gctatgctca cgtgccctgg atgaagaaaa accagaaact
    10741 gatccccgag tcacagttgc ccgatctcga cacccgcatt gcgctgcagc aactgctgct
    10801 cgaacggctg accgatgccg gctacctgta tatcggcatg gaccactacg ccagaccgga
    10861 cgacgaactg gtcagcgcgc aacgcaacaa gacgctctac cgcaacttcc agggatacac
    10921 cacccacaag aactgcgaca tccacgcatt tggtgtttcc gccatcagcc agaccgaatc
    10981 ggtctacgtg cagaatgcca agagtctgcc actctggcag gacaaggtgg gacacggtct
    11041 ggcgactgaa cgcgggctac gtatcgaacg tgaagaccgg ttacgccgtg acgcgattct
    11101 gcgcatcatg tgcgatctgg aactcgatct cgcggccttc tcgcgcgact gggacatcga
    11161 cgccaatgcg catttcgccg acgcgattgc ggaactgctg cccatgcaag ccgatggact
    11221 ggtcgaaatc gacccgcatc tcatccgcgt tacagaatcc ggccgcattt tcctgcgcaa
    11281 catcgccatg tgttttgacg cctatctgca taccggcgat ggcaacgatg aaccgcgcta
    11341 ttcgcgtacc ctctgaaacg gaacgccact tgccggaacc gctcagactg tccaagctca
    11401 tggctgcgcg cggtttgtgt tcgcgccgcg aagccgacac ctacatcgag cgcggctggg
    11461 tgcgtgtcga tggcaaggtc gtcgatgtcc ccggcagcaa agtggcgccc gactgccgca
    11521 tcgatctcga caccgccgca cagcgcgaac aggacgatct ggtcacgatc ctgctcaaca
    11581 aaccggtagg ctacgtctcc acccaggcag aacatggcta ccagcccgcc agcgtgctta
    11641 tcaccgccga ccggcgttac acccgtccgg acgagacgcg cagtttttcc aatcggcaca
    11701 cctggaatct ggccgtggcc gggcggctcg acatcgattc gaccgggctg ctggtactga
    11761 cccaggatgg ccgcatcgcc cgattgctga tcggggaaca ctccccggtc gagaaggaat
    11821 acctggtacg cgtcgaaggc agactgtcgg cgcaggggct ggaactgctc aaccacggcc
    11881 tgagcctcga cggcgtcgcg ctcaaaccgg cacaagtctc gtggcagaac cgcgatcaat
    11941 tgcgtttcgt gctgcgcgaa ggcaaaaaac gccagatccg ccgcatgtgc gaactggtcg
    12001 gactcaaggt caccggactc aaacgggtcc gcatcggcca ggtgaaactg ggcgacctgc
    12061 cgcccggcca ctggcgctat ctggcaaagg acgaacgctt ctcatgatct gcattcccgg
    12121 caaggacgcc ccgctcgaaa ccagcatcgc caccatgcag gcccgcctga ccgggcttgg
    12181 tttccacatt gaggaacgct cgtggctcaa cccggtcgcc ggcgtctggt cggtgcacct
    12241 gcgcgaacgc gactgcccgc tgctgttcac caacggcaag ggcgcctcac gcgaagccgc
    12301 gctcgccagc gcgctgggcg aatttttcga acggctcgcc tgccgctatt tctggacgca
    12361 ttactatctg ggtgaaacgc tgtccggcgc cgcgattgtg catcatcccc gcgaacgctg
    12421 gttcgcgccg gacccggatc aatgctggcc ggacggcgtg ctcgacgaaa cgctgcaggc
    12481 attttacgac ccggaaggcg aactcgaagc gcagcagctc gttgaccgca attccggcgc
    12541 catcgagcgc ggcatctgca ccctgcccta tgtgcgccac cgtgacggcc agacggtctg
    12601 gttcccggtc aacgtgctcg gcaaccttta tgtcagcaat ggcatggctg ccggcaatac
    12661 gccgaccgaa gcccgcaccc aggcgctgtc ggaaatcctc gaacgtcacg tcaaattccg
    12721 catcatccgc gaggggctgt gcctgccgga cattccagcg gaggtcattg cccgccatcc
    12781 gctcatcgag cgtggcatca acgaactgcg tgacgccggc ttcggcatcc tggtcaagga
    12841 cgcctcgctc ggcggtcgct atccggtagt caacgtcacc ttgctgcatc cgcgcgacca
    12901 gggctgtttc gccagtttcg gcgcgcatcc gcaattttcc gtggcgctgg aacgcgcact
    12961 gaccgaactg ttgcagggcc gtgcgctcga cgcgctggag ggatttcccg caccgggatt
    13021 cgacatggaa gaaattgccg acccgcgcaa cctcgaaatc catttcgtcg attcgagcgg
    13081 catactcagc tgggaatttc tgcgcgacac cccggaccat gaatggagcg gctggaatca
    13141 ccccggcagc acggccgagg aattcgaatg gctctgcgcc ggcctcgaat gcgacggcta
    13201 cgacatctac attgccgatc acgacgacct cggcgtctat gcctgccgca tcgtcgtccc
    13261 gggcatctcc gaaatctacc cggtggatga tctggtctgg gacaacaaca gcgccgccaa
    13321 tgacctgcgc gaagcattgc tcaacctgcc tgcgctcgac gccgtgaatt gcctgcggtt
    13381 gctcgaagcg ctggaggaac gcagtatcgc cgaccagcag ccggtggccg cattcatcgg
    13441 cctggctgcc gatcccggca gtctgtggcg cgaactgcgc atcggcgaac tgaaaacctt
    13501 gctggcgctg gccggcaacg accgcgatgc cgcgctggaa ggctgcgaat ggattcgcca
    13561 gttcgagcac attcccgaac agcgtcagcg cctgtaccgc tgcatcgcca ccttgctcga
    13621 catggacgat gcacagccgt atcaggccgc actcgagctg ctgtatggcc aggacaccgt
    13681 gcatacggcc acagcgatga tcaacggcaa actgcgcttt ccgggcctcg acgcgccggg
    13741 cctgcaactg gaaaactgcg tcatgcacca gcgtctgctc gaggcctggg gcaagatttc
    13801 gccctggacg cgtacgtccg gctgatcaac cttcgccctg cgtatcattc tccgacacgg
    13861 catgcaggat ttccacgttc tgtaccgcca tcggatgtgc agtttccgcc acggtgatgc
    13921 ggttcttgcc ggttttcttg gaacggtaca tgcgttcatc agccagatgc accagttgct
    13981 cccagctctg ggcattgctg tccgaacgtt cggccatgcc gatgctgaac gtcaccggct
    14041 tgccatcctg ccgcagcaac gtcagcccgt gcgcccgcag gatggtcagg atgcgtgatt
    14101 cggcctgcca caggctgatc tgtggcatga ccacgagaaa ctcctcgcca ccccagcgga
    14161 tgatggcatc gccgctgcgc aacgcatggc cgagcatctc gcccacccgg cccagcagat
    14221 aatcacctgc ctcatggcca tagcggtcgt tgaccaactt gaaattgtcc agatcgagaa
    14281 aagccaccgt cagcttctgc tggtgccgca ccgactgggc atacagaatg gccagcaacg
    14341 cctcgccact gcggcgattg agcaacccgg tcagtgaatc ggtgctggat tcctgataca
    14401 ggtctttcat cagctgcaac tggctcatcg aagccatcga actcaccacc gcgagcaagg
    14461 ccatcagcca gacaatggca aaatcgctga aacccggcaa cagcgagctg tggctaaata
    14521 acatcgaaac ggtaaacagc aacaggatcg gcaacgaaaa tgccagggtt tccagcgctg
    14581 ccagcgggaa tatcgacaat ccgcccacca gcacgaacgg caggaaggta taacccgcca
    14641 ccatggccga gctcaccgca ttcagatggg catcggtcag caaccagcga ctgaacagaa
    14701 aaaatatggt tggaatggcg aacagcaatc cgatcgcgat acgactgtga cgcggtgtcg
    14761 gtgtgcagcg gcacatggcg gcaagaaaca ggaaaatcag gctcgccagc acgcgcgcac
    14821 ccgacatgat ccaggccaca tcacgcggaa agccaatcca gtccaccacg atccacatgg
    14881 gcgtcagaat ggcaaacacc gccgagacca tgcgcacgcg cgacaggatg aaacgcgatc
    14941 gtctacgcgc gaacagcggt gcgtgtctgc ccggaaagat gaaatcccgg atttccccac
    15001 gtccgagcag gaaaaaatag ccgaaattat cgatcacata gcgaatcact gttttcatgt
    15061 cgcgcctacg gcacccgccg tcacccattg atcacatctg acaacatctt aatcgaatcc
    15121 gaagtgcagc gcatgcactc ttcaatcagc gatcaggcca ttgccgccac atcgccagtg
    15181 ccgacagcac gatcaccggc ccgatcatca cgccgatcag gccaaacacc tcgatgccgc
    15241 ccaggatgct cagcagcaca agcatgaacg gcgcctcggt acgggcacca gtcagtttgg
    15301 gtttgatgac gaaatccgca accagcacga ccgtattgct ggcaatgaaa aacacgatta
    15361 cgggcggcca tccctgcgca gaaagcagca ccagactgac gagcacgatc accagcaagg
    15421 cgccaagcgg gatcgtcgac agcaacgcga cggcgatcgc ccacacaggc cataccggca
    15481 ccttgagcac cgccatgatc atgccgatca gtgcgccatt gaacagcgtc acgccgagca
    15541 tacccagcat caccgaacgg atcgattggg taatcacatc gagcagcgaa cgggaaaatt
    15601 cggcatcgcc gctcgcccac gccatcatgc gcgcaatggc gctttccagc gccgcatgat
    15661 tgcgcgccac cgcctcggcc accaacaggg caataccgat atgcgccacc agggcccaga
    15721 catgatgcag cgaagacttg agctcgacgg cattggcaga agcccaggcc gccaaccgtg
    15781 ccgaggacaa tggcgtggca aaatcgttgg ccaggctgct ggcgagccag ccgccgaaca
    15841 ccggcaatga acggatctcc gccaggatct cgcctggagc cggcggcgcc ttgagcaggc
    15901 caatcatgac cggcagcgcc gtatcgacga tcaaccacac cggaaatgcc agcaacactg
    15961 tccacgacag ggcgtgcagg gccgcggcca caggactgga gcaacccagc cgctccagcg
    16021 atttctggta gggcatgccg gcaatcgcca tacagaatcc tgccaggatc ggcaccgcga
    16081 atggtgacat caccaccgtg gccagcaccg caattccggc ggcaacaact tttcttgaaa
    16141 ccagcatgcg tacctttcat gttgcccagt ccacccgaac gactgaccag cctgtcagct
    16201 aaaatacaga ctcaaatcaa ctctaccgca tatgtgctgt tcgcgaaacc acttctgcac
    16261 acatttcacc aatcgacaag tacacaggtt ctcgttacca tagcgccaga ctgcaacgac
    16321 ccgaaatcgt ccggtcacat ttcgccgacc ccgccatttt ccgccgagcg atccgcaatg
    16381 tcacccattg aatccgatac accagccgtg ccccctgccg actcggaccc ggccacccgg
    16441 aatgccgcca cgcacagcac actggtcagc gccgcagtca acttcgccct gagtctggta
    16501 cagatcgcca ccggcatgct gtcgggctcg caaggtctgg tggccgacgg cattccctcg
    16561 ctctccgacc tggtggccga ttttgcggtg ctgttcgccg gccaccacag ccgcaaggtc
    16621 gccgacgccg atcaccccta cggccaccag cgctacgaaa ccgcggcttc gctgctgctc
    16681 ggcctgttac tgctcgccgt cggcgccggc atgctctggt cggcagtcgg caaactggaa
    16741 aacccggatg gcatacccac tgtgcgcctg gccgcgctgt gggtggccat cgcgtcgctg
    16801 gtcaccaagg agttgctgtt ccgctacatg ctgggtgtgg cacagcgcat caagtcgagc
    16861 atgctggtgg ccaatgcctg gcatgcccgt tcggatgcag cctcttcact ggtggtcggg
    16921 ctcggcatcg ctggcaacct ggctggttat acgctgctcg atccggtggc cgcgctcatc
    16981 gtcggcggca tgatcgcccg catgggctgg caattcggct ggaatgcgct gcaagacctg
    17041 atggaccgcg ccgtagacga ccaggaagtt gcagccatcc gccagaccct gctcgaatcg
    17101 cctggcgtgc tcggagtgca tgatgtgcgc acccgcaaga tgggcgacat gatcgtggtc
    17161 gatgcccaca tcgaagtcga tgccgacatt ccggtcgcca ccggccacga cattgccgtg
    17221 accgcgcgcg aacgcgtcat gcagcgccat cgcgtgctcg acgtgatgac ccacatcgat
    17281 ccttggcaac gcccggatct cgatcacgag catttttcag ggcagtgagc tgcggcccga
    17341 aggtgaccgc ggtggccagg gaccagtctt ccggtcatgc agcagcatgc ggagcgcagg
    17401 ctgaagtctg acgcgcttcc acgctgacaa atatcccggg ctcaatgccg agcggggtgt
    17461 agacatgccg attcgccaga gcgatgatgg cggaaaaagc atcacaaagc atccgaatgt
    17521 cgtcgccttc cgattccggg ccaaatccgt gtgtcatatg tgccaatccc ttgttcgcaa
    17581 ggtacagcat gtgggcgaac ccgcgttcca cccgctccgc tgacagcccg tcgtgtttgt
    17641 cgagcgcgtc ggcaaccgac ggcatcgata agccttccat atcctcgata catgcgtcgt
    17701 cttttctgcg gctggttacg tttactactt ttctgcggct ggttgctgtt gctctttgtt
    17761 gctgcggatc ttcttcgctg ccgatcctca agccaatgaa ctcgagcact gcacgcccat
    17821 gcaggatacc cgcctccagc acagggttcg tgtagccagt agcgcttccc tgagcaacga
    17881 gcacgtggtc aaaacggatc tgcatcgatg gcggcgtttc ggcccattca ttcatctgca
    17941 tcagcacaat gagcgccgac gtgatggcag acaccctgta gtcaagccac gcaacaacgg
    18001 cttgttgtcg atcctgtgtc acgtcacgcc ctcctgaaac aggtcgcaat tgttggtcat
    18061 gagcgctgcg aatgcacgct acgtctttcc aggcaacccg gcagcggtct tgtgcatcaa
    18121 caaagcagca atgaacgcgt catcgtcaaa tagcgcatcg attccattat cgagcgccat
    18181 attcaaggct gcttctgcat ctcgcgaccc catggtcgca gcccatgccg tgcgaccctg
    18241 tgcggcaatc tgacgcgaga agcggttttc cccgttcgaa agcatcttca catgcatatc
    18301 caggtccgca acggcatccg agcgaaggaa gccgaccttg taatcattga agaacgtatt
    18361 tatgtcgatg acaatttgca gatttgcgtc atcagcgaca cgaaatcccc gggctttgag
    18421 ctcactttca acggcttgtc tgatggtctg ttcaacgggt ctggttacaa tgatgtcagg
    18481 gtaggtttcc tctcccagcc cattccgccc ataccccacc acggctttcc cccacttcct
    18541 ggatatcccg ggagccagat agtcacgctt gtcattgact tgcaccgcga caacgatgtt
    18601 cgcagcctct ggaattgtat taaccttgag ttgcgtgtga tacaaagtga tgcgttcttc
    18661 cctgatgatc cacgtcgcac atccggcaac gagacttgtc aaagccagta cgcaggtcat
    18721 catgatgatc ggtattgcga accggacaca ttccgggtcg cgccatggcc acccgttcct
    18781 gaaatgcttc atctggcctc gctggaaggt atgctgaaac ggctgcagtc agaaattgaa
    18841 gccattgtgg tccgtctgca accggtttgc aacgtaacgc ccacgataaa atggcttgcc
    18901 acgacacgcc accttaacaa ctgctaaatg atcgcgtgct gccggtcatg accatcagta
    18961 tccttgccgg atatcttgca cctcgcccag tggcacatga aagaccggag aactgcttcg
    19021 accaaccagc gaactgtcgg gtttatcaag gctccgactg cgttgtgaat ccgtcccatc
    19081 aagcaggaac atgcacacca tctgcaccag caccggtttg cgcttgcggt ggcagcggaa
    19141 tcgacgacac atgtaacgca ttggcaacct cgcttcaatt ttagttgtat aatcaaaaca
    19201 tggaacagcg cactgccatc accttgctcg aatcgctggc ttcgggtgtc cgactcgaca
    19261 tctatcgctt gctggtgcgg catggcccgg aaggactggt ggccggtgaa atcgcaaccc
    19321 ggctcgatct gccggccacc aacctgtcct tccatctcaa ggcgctgacg caggccggct
    19381 tgctgagtgc ggtacaggaa ggccgttttc aacgctaccg ggcgcatgtc cagcccatgc
    19441 tcgacctggt gagttatttg actgcggaat gctgtggcgg tcgcccggaa ctgtgcgcac
    19501 tgccctgatg gcatgcgcgc gacggtacgc actgccttgg tggcgtacgc gcgatgctgc
    19561 gcgcgccttt ggtgacgtac tcgcgatgct gcgcgctgcc ttggtagcgt acgcgcggtg
    19621 ctgcgcgtac aatatttcaa ctattgatga attattgcat catatgacct cgcccctgtt
    19681 tgctccatca accggatcgc acaaggagtc cgacatgcac acccttccct ggaacgtact
    19741 gttcgtctgt accggcaact cggcacgcag catcatggcc gaagtcatca tgaaccagct
    19801 gggcagcggg cgtttcaagg cgtacagcgc cggcttcaag ccccggggta gcgttcaccc
    19861 catgacgctg gacattctcg cccggcaacg ctacccggtg gacggattgc gcagcaaaag
    19921 ctggcaggaa ttcctccggg acgatgcccc acgcatggat ttcatcttca ccgtctgtga
    19981 tcaggccgca ggcgaactgt gcccggtctg gcccgggcaa cccatcacgg cgcactgggg
    20041 tttttccgac ccggcggccc tgaccggcac acatgaacaa caattccgcg cctttttcga
    20101 cgtgcagcag cagattgcca accgggtaaa gctgttcatc agcctgcctt ttcacaagct
    20161 cgaccgcctg gctctgcaca accggctgcg cgacctgggc tgctgaaccc tcgcgatgcg
    20221 ccaggcattc gcttccggcc tgcatccatg accacccccc atcaattccc gacaggagcc
    20281 caccccgtga ccgtccagtg tgaaattgcc gccaggcagg caagcggcgc ccccatgaac
    20341 cgtttcgagc gctatctgtc gctgtgggtg ctgctgtgca tcgccgccgg cattggcctg
    20401 ggcaagggtt ttcccgccgt gttccaggcc atcgggcgca tcgaattcgc ccaggtcaac
    20461 cttccggtcg gcctgctgat ctgggtcatg atcattccga tgctgatcaa ggtggatttc
    20521 accgcgctgc acgaagtgcg ccgccacgtg cgcggcatcg gcgtcacgct ggcggtcaac
    20581 tggctggtca aaccgttctc catggccttc ttcggctggc tgttcctgcg tcatgcgttc
    20641 gctgcctggc tgccgccagg ccagatcgac agctatgttg ccggcctcat cctgcttgcc
    20701 gccgcgccat gcaccgccat ggtgttcgtc tggagccgtc tcagcggtgg cgaccccctg
    20761 ttcacgctca cccaggttgc gctcaacgac agcatcatgc tggtggcctt cgccccgatg
    20821 gtggcattgt tgctcggcat ctccgccatc agcgtgccat ggtcgaccct ggccgtttcg
    20881 gtgggcatct acatcctgct gccgctcgcg ctggcgcaat ggtggcgcaa atcactgctg
    20941 acacgcggcc aggcagcttt cgacgccgcc atgaccgcgg tcgggccatg gtcgatggcc
    21001 gccctgctgg ccacgctggt gctgctgttc gcctttcagg gggaatccat cctgcagcaa
    21061 ccgttgatca tcgttctgct tgccataccg attctggcgc aggtgctgtt caatgcatca
    21121 ctggcctacg gactcaaccg tatcctgggc gaacgccacg acatcgcttg cccgtccgcg
    21181 ctgatcggag cctccaattt cttcgaactg gccgtggcga cagccatcag tctgttcggt
    21241 tttcattcgg gagcggcgct ggccaccgtg gtcggcgtgc tggtcgaagt gccggtaatg
    21301 ctgctggtgg tgcgtgccgt caccgccagc cgcgcctggt acgaacgtgc cagctgacaa
    21361 taataaacat gccgccagcc atacgtcatg ctccactcag ttccgcgcaa gcatgattgg
    21421 gtagcatcag catcggatgc gcgaccatcc ggactcgcgc ccgattctca aggagaatgg
    21481 atcatgaaga tgaaacacct ggccgtgctg atgcttggct gcaccctgtt tgccggcaat
    21541 ctggctctgg cggacgaggt cgtgggcacc gaaacgctgg gcatctcggt caacgaagac
    21601 cagatcgttg ccttcggccg cagcgtgacc aaagaaatcc tgcacaagga cgtctacaac
    21661 gaccacaagc agaagctggg cgtcatcgaa gacatcatcc tgtccccgga acattccgcc
    21721 agctatgccg tgatcgacat cggcaatttc ctcggcatcc cgcgccaccc ggtgctggtg
    21781 tcattcagcc gcctgcaccg cgaacatgac agctacgtac tcaagggtgc aacccgcgcc
    21841 gcaatgaaat cgctgccgac cttccactac aaccccgcga acaaataacc cctccacgcc
    21901 gttccgatcg cacccgcaac cgcgggtgcg atgccacatt ccctgtcccg cgaactttca
    21961 cgccaacctg ccctccaatg ttgacagatg gccgcgcaca ttgcatcctg acaggccatt
    22021 gacagccatc acgaacgcag gaaggtcaga aaatgcctga aatccaacgg gatacgtaat
    22081 gaacaaggat tttgcgccca ttctgatcaa cttccggcga gcaacgccgt gtcgcaacag
    22141 cctgtcaaat cccgtcccgg caatctcccc gttatgaaca ttggagtgac aatcatgaaa
    22201 aaactgctac tgatagtaat actgctgggc aatctggttc aacctgccct ggccgacggc
    22261 tggcatggtc gcggcggctg gcatggtggc tggcgcggtg gcgatggctg gcgtggtggc
    22321 gacggctggc gtggtggcga ctggggaggc ggcgccctgg cctgggggct tggcctcggt
    22381 gtactggccg gcgcgattgc aaattcgcaa ccgacgtatg catcgcctcc gcctgtgtat
    22441 tatcagcagg cgccggtgta ctactcgcag ccggcaccgg gttattatcc ccaacaggcg
    22501 ccagtctatc aggctccgcc acccgtcatg tacggtccga ctgtctatta tcacgatgat
    22561 gactgagcgg attccccggc tgctcccggg cgtctccaat cagcgacata aaattaatcc
    22621 tttatattca aacacctgat ccacaatccc ccactgacat caagaccacc tgtctccgaa
    22681 cggagacagg cacccccctc cctggcgagg cggccacaaa aaacacgaac aaataatcat
    22741 tcaatttcaa caagttgcaa taaaaattcg atctggcccg attcgtgctt gaaaaaagcg
    22801 tcagccactt tcaagccaag acatgaaacc gcttcctgcc ctgccttcgc tgcgcatccg
    22861 gacatttccc ctcattcacc gcggcagcat ggtcgcccga ccctggaccc gcttctggcg
    22921 cggcgcgctc gatctgctga ccgggaacgc aaaacacccg gatctggcag cagccagcct
    22981 gccctggaaa cacgcagcgg cgcagcgcag aaccatcctg ctgacactgg tgaccgcaat
    23041 ttcggccacg gccgcagcgc tgctgtatgc aaacacgccg gcacatcagc cacatccgct
    23101 gctgcacttc ctgcaactcg cgctgttcgt gctgctgttc ggctggatat cggcaggctt
    23161 catcacggcg atgatggggc tgtgggtaca gtggcgcggc gaccgccatg ccctcaggat
    23221 cgatgcctgc gccagccagc cgctgtccgc agaagcccgt accgccatca tcatgccgat
    23281 ctgcaacgaa cccgtcgagc cggtgtttgc cggtttgcgt gccacatgcg aatcgctggc
    23341 ggcaagcgat ggcgcagatc tgttcgacgt cttcatcctc tccgacagca acgacccgct
    23401 caccagtgcc caggaactgc gcgcctgggg gatgctgcgc cagcagctcg gagaacgctg
    23461 ccgcctgtat taccgccagc ggctgcgccg cactcgccgc aaggcaggca acgtcgccga
    23521 cttctgccgt cgctggggca aggactaccg ctacatggtc gtgcttgacg ctgacagcgt
    23581 catgaccggc gaaaccgtca cccagctggt tcgggcaatg gaagcccatc cggacaccgg
    23641 catcatccag acggctccgc gcgcctgcgg ggtcgacaca ctgcacgcac gcacccagca
    23701 attcgccggc cgcgtggttg gccggctgtt cactgccggc atgcaattct ggcaactggg
    23761 cgaatcgcat tactggggac acaacgccat catccgcgtc gcgccattca tgcagcactg
    23821 tgcgctggca acattgcccg gcacaggcgg cctgtcggga gacatcctgt cgcacgactt
    23881 cctcgaagca gccatgatgc gtcgcgccgg ctaccacgcc tggctgacca ccgaccttga
    23941 aggcagctat gagcagcagc catccaacct gatggaagaa ctgcaacgcg accggcgctg
    24001 gtgccagggc aacctgatga atttccgcgt cattgccgaa cccggctttc agccggtgca
    24061 ccgcgccatg ctgctcaccg gcgcactgtc ctacgtatcc gcaccgttgt ggctggcatt
    24121 cgtgctgctg agcctggcgc tgcaactgat ggaaccgccg gcaagcggga gcgcactgtc
    24181 ggtctggttc ggcgcatcac ccaccctgac catgctgtgg ggcctcaccc tgagcctgct
    24241 gtttctgccc cgactgctcg cggtgatcgg catcctgctc aagcgcgaac aggccgggtt
    24301 tggcggcacc gcatcattgc tgaagagcgc cctgctggaa ctggcgattt catcgctgca
    24361 ggcgccgatc cgcatggttg cccacacgct gttcgtgttt ggcgcactca gcggcctgaa
    24421 cctgaaatgg aaatcgcccg gccgcgaatc acgttcggtg gcctggaacg atgcctggct
    24481 caggttcctg cccgtcgccg cactggtgct gccgggactg ctggcattgc tgtacacgca
    24541 gcgcgacacg gcgctgtggt tgctgccaac ggccttgccg gtgctgcttg ccgcgccgat
    24601 tgccgtgttc accagccatg cgcactgggg cgtgctgctg cgccgccacc gctggctgct
    24661 gactcctgaa gaacgcttca cacccgccat tcttgcccgc acattcgcct gaacatgaca
    24721 gacactgcag cccctgcggc cgggttcaag ccactccgcc gccgttttct cgcgctgctc
    24781 gcgctgggca ttgcctcgcg cagctggtcc agcgacacca gcgtaccgga tacgggcacc
    24841 acgacaaccg cgacgccttt ttccttcgac gggctcatcg ccgccgcaca ggacctggcg
    24901 cgacatgact tcgtgcccga cctgcaaccc gatcaggccg tactcgatcg catcgactgg
    24961 aatgcgcacg gccagataca tttcaaggcg gataaagccc tgtttgccga cggcccgggc
    25021 cagtacccgg tcgaattctt ccatcccggc cgttttttcc gcaaccgcgt gcgcatgtac
    25081 cgcctcgacg ctgcgcttga tgcagcacac ccgaccacgc aggcccacga aatcccctac
    25141 agcgccgacc tgttcgacat gcctgcagac agcccggcac acgccatgaa cgcacagcac
    25201 gccggcttcg ccggtttccg tgtgcaggaa agccgcaccg gcggcccggg ccagccggac
    25261 tggcgcacca acgactgggc ggcgtttctc ggtgcttcgt attttcgcgc catcggcgac
    25321 cagtaccagt acggcctgtc ggcacgcggc gtggcggtca ataccatcga aaacggcgtg
    25381 acagaagaat ttcccgcgtt cacccgcttc tatttcgaac ccggtacggc cgactcgcac
    25441 accatcgtca tctatgccct gcttgacggc ccaagcctgg ccggcgccta ccgcttcacc
    25501 atgacacgcg aacaggacgt acgcatggac gtcgaagcgc ggctgttcct gcgccgtgac
    25561 atcgcccgct tcggcattgc accggccaca tcgatgtact ggttttccgg caaggacaaa
    25621 ccgcgtcaga tcgactggcg accccaggtc cacgattccg atggtctggc gatgtggacc
    25681 ggacgcggcg aacgcctgtg gcggccactc aacgatcctg ccggcatccg cctttcggta
    25741 tttaccgacc acaacccgcg cggcttcggc ctgatgcaac gccagcgcca ctacgaccgc
    25801 tatctggatg ccgtacatta tgaacggcgt ccaggactgt ggatcgaacc gctcgacgac
    25861 tggggtgacg gcagcgtgca actgatcgaa ctgcacaccg cggaagaaat ttacgacaac
    25921 atcgtcgcca tgtgggtgcc tgcaactgca gccactgccg gttcgagcca caccctgcgc
    25981 taccgtctgt actgggccgc aaacgaaccc gacccgggtc cgctggcccg ttgtgtcgca
    26041 acccgcatcg gacgcggcgg cgaacctggc tcgcgtccgg ctgattcggt actgttcgtc
    26101 gtggaatttg aaggcgcatt gctggacagc ctgccacaga atgtccagcc acaggccgac
    26161 atctccgcca gccgcggtca ggtcgccgcc atcgcgatcg aacgcgatcc ggacggcaat
    26221 ccggcgcact ggcgcgcgcg ctttgaactg cgtcacgcac cagccggcga tacgcccgta
    26281 gaactgcgcc tggcattgcg caacgctgat cagatactga gcgaaacctg gctctatgcg
    26341 cacccggtga cctgaacacg gtccgaggca cggatcaggt tcatttcacc gctatttgcc
    26401 gggagcctgt ccgtcgtccg tctgctcgat cacctcgtgc gcgagttcgg caatctggtg
    26461 tcgcaactcc tgcatggcct gcgcaccctc ggcggtggcg cgataatatt tgcgcatttt
    26521 tccgcccacc acttcccgat cggacaccag atagccctcg cgctccatcg cgtgcaataa
    26581 cggatagagc gtgcccgggc cgataccgta tccatgccgc gccagttcct ggatcagttc
    26641 cagaccgaat atccgctccc gcagcgcatg gtacagcaca tgcacgcgaa tgaaactcag
    26701 ggtgatttcc cgcagaaaac gcatcgcatc ctcatttgac agccatgccg atggccaggc
    26761 gcgccaccac agcgcatctt atcactgcgc tgccggaatg aggattttcc ttgccgcggc
    26821 cagcgaaggg gttccgccga ctgcatcacc gatacgctac tctgcgtgct tgtggatcgc
    26881 gccgatgttg ccccacagcg acagatcgtc cacttcaacc ccgatccacc acagacgacc
    26941 gaggctgtcg cggtcgacac caccgacgac acgctgcccc tcggcaagca cgatgtggct
    27001 ggcgaattca tcggcgcgtt ccggatcacg gaagacgtgc cacatcacca cccgcgtttg
    27061 caatccgcgc accggcaggc cgcg
//
LOCUS       DAIANL010000035        27638 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1261525, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000035 DAIANL010000000
VERSION     DAIANL010000035.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 27638)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27638)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27638
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261525"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5202 a         7872 c         9477 g         5087 t
ORIGIN      
        1 cgagcaggac atcatcgagc gcttcgctgc gcattcgcgt gttccggtca tcaatggcct
       61 gaccaacgaa taccacccct gtcagatcat ggctgatatc ttcacctata tcgagcgtca
      121 cggcagcatt cagggcaaga cggtggcctg ggtgggcgat tcgaacaaca tgtgcaacac
      181 ctggctgcag gcggccgtgg tactggattt caacgtgcac gtatccacgc caccggggta
      241 cgaagtcgaa ccggagcgtg cggggctgta cgacaccacg cattacgaac agtttgacga
      301 cccgatggaa gcctgtcgcg atgcgcatct ggtgaccact gacgtgtgga ccagcatggg
      361 tttcgaagcg gaaaacgaag aacgcaagcg cgacttccag gactggcagg tggatgcgga
      421 catgatgcgc gtggcccggt ccgatgcgct gttcatgcac tgcctgcccg ctcaccgcgg
      481 tgaggaagta acggccgaag tgatcgacgg cccgcaaagt gtggtgtggg atgaggcgga
      541 aaaccggctg catgtgcaga aggcgctgat ggaatacctg ctgctcgggg agcagcactg
      601 atttttgtgg tctgccatgg agcgaacggc aggttcaggg aggataaatc tggatgaaaa
      661 gagcatgagc gacgtaaaga aagtggtgct ggcatattcc ggcggactgg atacctcggt
      721 gatcctgaag tggctgcagg acacctacca ctgtgaagtg gtgacgttca ccgccgacat
      781 cggtcagggc gaggaactcg aaccggcacg cgacaaggcg cgcgcactcg gggtgaaaga
      841 aattttcatc gatgacctgc gcgacgaatt cgtgcgggat ttcgtttttc cgatgtttcg
      901 cgccaacacc ctgtatgaag gcgaatatct gctgggcacg tcgatcgcgc ggccgctgat
      961 tgccaggcgg ttggtcgaga ttgccaatct gaccggagcg gacgccattt cgcacggtgc
     1021 caccggcaag ggcaacgatc aggtgcgatt cgaactgggc gcctacgcgc tcaagcccga
     1081 tgtgcgcatc attgcgccgt ggcgtgaatg ggatctgacc tcgcgcgaaa aactgctggc
     1141 ctacgccgaa cgtcatggca ttccggtgga aatgaagcac aagagtggtg gcagtccgta
     1201 cagcatggat gccaacctgc tgcacatttc ctatgaaggt cgtgcgctgg agaatcccgc
     1261 cattgaaccc gaggctgaca tgtggcgctg gactgtggcg ccagagcagg cgcccgacag
     1321 cgcagaatat gtcgaactcg aattcgtgcg cggcgatgtt gtggccgtga acggcgagaa
     1381 acttggaccg gctgcggtgt tggccgaact caaccgcctg ggcggcaagc acggcatcgg
     1441 tcggctggat ctggtggaaa accgctacgt cggcatgaaa tcgcgtggct gttatgaaac
     1501 tccgggcggc acgctgttgc tcaaggcgca ccgcgccatc gaatcgatca cgctggaccg
     1561 cgaagtggca cacctcaagg acgagtgcat gccgcgttat gccagcctga tttacaacgg
     1621 atactggtgg agcccggaac gccagatgct gcaaggcatg atcgatgcca cccaggagcg
     1681 cgtcaacggc tgggtgcgcc tcaagctgta ccgggggggc gtgatggtgg tggggcgcga
     1741 ttcggcgacc gattcgctgt tcgatccgac cattgccacc ttcgaggacg atgcgggcgc
     1801 ctaccagcag gccgacgccg ccggtttcat caagctgcat gccctgcgca tgcgcatcgc
     1861 tgccagaagg agggcatgat gtcacaattt gatcaggtga gcgtagtcaa gcgcgccaac
     1921 gtgtattttg atggtcagtg catttcgcat accgtattgt ttgccgatgg gagccgcaag
     1981 accctcggcg tcatttttcc cggaaaactg gtgttcaata ccggcctgcc cgagatcatg
     2041 gaaatcaatt ccggtcgctg ccgggtgaag ctgccgggcg agcaaggttg gaaagctatt
     2101 gaggcgggcg gtcaattcag cgtgccaggt aacagccagt tcgagatcga gacgctggaa
     2161 ctgctggatt acgtgtgcca tttcgtgcag gaagcctgaa cgtggtacga ccggcggccg
     2221 gaattgaagc catcccgggg catgggcgtg cagccacagg cacggggatg atttcatgtg
     2281 ccaggagtgc cagttgtggt gctgtttttc ttgaccgcgt ggccagagcc ctggccaaag
     2341 gctacagcag gatggagtga tcatgcccag tttcgatatc gtgtccgaag tggacaaaca
     2401 ggaagtgcgc aatgcagtgg agcaggccaa caaggaagtc tccacccgtt ttgatttcaa
     2461 gggatcggac gcgcgcgtcg agcagagcga atacgtgctg accgtctatg ccgacgacga
     2521 attcaagctc gaccaggtgc aggaagtgtt gcaggccaag ctcatcaagc gtggcatcga
     2581 catcaaatgt ctcgaaaccg gccaaccgga aaaagtgtcc ggcaacaagc tcaaacgtgc
     2641 cgtgaccgtg aagaccggag tcgaatctac gctggcgaaa cagatcgtca aacttgtcaa
     2701 ggacagcaaa ctcaaggttc aggccagtat ccagggcgaa accgtgcgtg tcacaggcgc
     2761 aaaacgcgat gtgctgcagg aagccatagc gctggtgcgc aagcagatca gcgatttccc
     2821 tctgcagttc cagaatttcc gcgattgact gctgcgcacg cattggggca atccggcagc
     2881 tgacgtggct gcgtagcccc gcttccgcgt ccttgatggg ggcagcatcc cgcacgctgg
     2941 caaacccggg cggcggcttt tcagactgtg ggccgagagc gcgttatgcc gggttcctga
     3001 gcatgcccaa tcattacgcc accgtgtgcc agccatgcca caaagtgctg ccacgcaatt
     3061 ttccgcaggc ttcctccccg catgccacaa ggctgtctcc cggctttcat cacggcgggc
     3121 cttctggtat gcttgaccag catgaacaga ccggtgcgag gatataaatg atcatacgtg
     3181 acattctgac ggtgaaaaaa gacggcgtga ttttttccgt cgcgcccgac cagaccattg
     3241 cggaagctat tgatctgatg gtgaaatcag atattggatc gctggtcgta catcgtgatg
     3301 gccatatgac aggcctgttg actgagcgtg acgtgctgcg tggcatcagt cgtgagggcg
     3361 cggccggact gggtcggccg gtgagcagca tgatggtcgg ggagccgatt ttcggcagtc
     3421 cggatgacac cgtggactac gttcgcggcg tgatgacgga aaaccgcatc agccacctgc
     3481 tggtcatgga aggcgacaat ctgctcggcg tgatttccta tcacgatgtg gccaaagcct
     3541 gtctcaacca ggcggatttt gagaacaccc tgctcaagcg ctacatcaaa cactggccgg
     3601 aataaggcac agtggccggt tagcgcatgc gtttcgcttc cagttcaccc aggatatcga
     3661 gataggcggc gagccgcgcc ggcaggattt cgccgcgggc ggcagcttcg cgtacggcgc
     3721 agccgggttc cagatggtgg cgacaattgt cgaagcggca atgaccgatg tggggacgaa
     3781 attccgggaa caggtgatcg agcccggcgg cggacacctg atgcaggcca aacgtctgca
     3841 tgcccggtga atcgatgatg cggctgtttt cgtcgatacg gtacatgcgc gctccggtag
     3901 tggtgtggcg tcccgaatca agtgccagcg agatgtcgcc ggttgcgctt gcagcgtcgg
     3961 gcaccagggc attgatcagg ctcgacttgc ccatccccga ctgaccaacg agtaccgtga
     4021 cccggcctga caggcgctcg cgcagtgccc gcacatcgtg gcgggctgat acttgcagca
     4081 cgtcataacc aagtgcagca taccccgcca gcgcgcgtgc ggtggcagtc accgcttctg
     4141 tcagatccga cttgttgata cagattgcgg ccggaatacc tgcggcttcg gctgcgacca
     4201 ggcaccggtt gagcaacgtc tcgtggtagc ttggcaatgg ggcgaccacc acgaccacct
     4261 ggtcgacatt ggcggcaatc agcttggaac ggtgtgcgac cgaacggtag agcaggttgt
     4321 cgcgtggcag gatgcgctcg atgaccccct gaccgggcga agtcggccgg acatccacac
     4381 ggtcgccgca ggctgcaccg ccctgtttgc cgcgcatgac acaggccagt tgcgtatgct
     4441 gcgctgtttc caccagcaac tggtgaccga agctgccggt gacccaaccc tgaagcacgt
     4501 tcatgaatcg agggccttga gccggtagag cagttcgagc gccgcgcgcg gcgtgagttg
     4561 gtcagggtca gtctcgcgca gcagttgcat tgcgggcgat atgtttgtca ccgtgggtgt
     4621 caggctgaac aggtccggtt ggcgtggcgc agattcctgt tccagttcgg cgagcttttt
     4681 gcgggctgcg acgatcaccg tttcgggtac gcccgccagc gcagcgacct gcagaccata
     4741 gctgcggctg gccgggccct cctcgatgtg gtgcaggaac acgatgccgt cacggtggtc
     4801 gcgtgcttcg acatgcacgt tgctcacttg tggcaacact tccgccaggc gcgtcatttc
     4861 agcgtaatgg gtagcgaaca gggtccaggc gcgcgtctga tccgccagtt gccacgcgac
     4921 cgaccacgcc agcgccaggc cgtcgaaagt ggatgtgcca cgaccgatct catcgagcag
     4981 taccaggctg tgctgcgtgg catgacgcag gatgtgggcg gtttcggtca tttccaccat
     5041 gaaagtggaa cggccgccgg ccagatcgtc ggaactgccg atgcgggtga agatgcggtc
     5101 gatcgggccg atgcgcgcct gcgttgccgg tacatggctg ccacaacagg ccagcaggac
     5161 gatcaacgcc gtctggcgca tccaggtcga cttgccgccc atgttggggc cggtcaccag
     5221 caacatctgc cgttcgcgcg tcaggcgcac accgttaggg atgaacggat gcacgcggct
     5281 ttccactacc gggtgacggc cggcgcggat gtcgattccc ggctcgtcaa cgaattcggc
     5341 cggggagaaa tccagtgctt cggcgcgttc ggcgagcgtg cccaccacgt cgaggtccgc
     5401 cagcgcatcg gccaggcgca gcagcgcggg aatgtccggg cgcagcgatt ccagcaactg
     5461 ttcgtagagc agtttttccc gcatcagcgc acgttcggct gccgacaggg ctttatcttc
     5521 aaacgccttg agttcgggcg tgatgtagcg ttcggtgttc ttgagtgtct ggcggcgcag
     5581 gtagtcagct ggcgcgtgct cggcctgggc gcgggtcagt tcgatgtaat agccgtgtac
     5641 ccggttgtat tccacccgta gcgtggcgat gccggtgcgt tcgcgctcgc gcgcttccag
     5701 atcgaggagg aatgcgccgc tgtgctgctg caatccacgc aactcgtcca gttccatatc
     5761 gtagccgtcg gcgatcaccc cgccgtcgcg cagcaccagt gacggttcct ctcgaacggc
     5821 acgcgccaga cggtcgtaca gggctacagg tggcacgcag cggagatgca gcgcatccag
     5881 ccaggcactg tcacccgcga cagcagcgca ggtttcgcgt agcgcaggga tgcgtgccag
     5941 gctgtcacgc aggcccgaca ggtcgcgcgg gcgggcattg ccgagcgcaa ttcgactggc
     6001 gatgcgttcg atatcggcca gaccttgcaa ctgggcgcgc atggcgctgc gcatgctggt
     6061 ggcggcaagc agcacatcaa tcgcccgctg acggtcgcgc aggcggtcat ggtcgcgcag
     6121 cggatggtgc agccagtggc gcaggcggcg tgatcccatg ctgctggtgc aggtgtcgag
     6181 caacgacagc agggtgggtg aagattcacc ctgtaatgtc aggtcgattt ccaggttgcg
     6241 tcgtgtggcc gcgtccatgc ctacgtactg ttcatcgtct tccagcgtca gcttgcggat
     6301 gtgcggtaac ccgccggatt gggtggacag ggcatagtcc agcagggcac cggcagctgc
     6361 cagcagtgcg ggtgacaggt gatcgagttc ggcgtcgtcg aactgttcgc gcagtcgcgc
     6421 gtgcgcgtgc gttgcatcga acaggccgga ggcgagttcg cgcagtgcgc cctgaccggt
     6481 cagcgccgga tggcggaagg cctgacgcac gagaatctct ccggcaccga tgcgttcgag
     6541 cgcaccaccc agctgatcgg ctgcgcattc ggccacggca aaacgtccgg cagccagatt
     6601 gagccaggcc aggcccagag gggcatcgtt gctggccggc gcgatgcaca acagcggccg
     6661 gtcgcggcgt tcgtcgagca atgcactgtc ggtgagcgtg cccggcgtga cgatgcgtgc
     6721 tactgcgcgc tgcatcggcc cttttccggc cggatctccg aactgttcgc agatggccac
     6781 cgattcgccc aggcgcacca gccttgccag atagccgtcc gcggcgtgga agggcacacc
     6841 agccatcgga atcggttgtc cggccgaggt gccgcgccgt gtgagcgtga tgccgagcag
     6901 ctgggctgcg cgttcggcat cgtcaaagaa cagctcgtag aaatccccca tgcggtagaa
     6961 caacagtttg tcctgatgtt cggatttgat gcgcagatac tgctgcatca tcggggtgtg
     7021 ctggctgaag gtggtcggat cggtcaattc aatatgcttc ggagaggcgg aacgatggaa
     7081 tgctgcacat tatcgcgtga cctgacaggt tcgtccatgc ccgggcagtg atgctgccgt
     7141 cagggggtat gaccagcagc atccgccggt agcggtgtcc agtcgctctg tgcgtccagg
     7201 cgcagataat agcggccggc gtgttcgagt acgatggtgt cagcgttttg cgacaccagc
     7261 cgggtggtgg gcaggtcgcg cgcataggtg gcggggtcaa atcctgcacc agtgaacggt
     7321 ggctgctgca acaggtgttg cagcaccgca ctcatggcca gataactgga gggctggtcg
     7381 atggtcacgg aatcaccgtt gcgctgggac gggccgatga tcttgattcc gaccgggacc
     7441 cgggtgatcg aaggggtggg gatgtcgcgc aggcccgcaa tctgcatgct gtcgcctgca
     7501 accgcagcgc catgctcggg gatcagcacc agcacgacat tgcgtttcga agcagcgatg
     7561 cggtccatga acgtgccgag atcgtcgagc agccgcgtca ggcgctggcg gtagaggtcc
     7621 agcgagccgc cgttgctgtt ctggccggtg atccggttgc cgtcatgcag gctgagggtg
     7681 ttgtagtaca gggctacgcg gctggcggga tcggtggcgc gccgatcgag ccagcgtgac
     7741 aggacatcga gatcgtcgcg gatgggcgtg ttgtcgaagg cgcgctgggt caccggcaga
     7801 ccgtccagcg gtagcggtgc cacatccagc ttctgttgct gcacaagctg gagaaaattg
     7861 tcgaaatggc catcatgatt gagtgcgagt tcgcgttggt agccgtcctg ctgcaggtcg
     7921 cccatgaggt agcatgcatc ggttgctggc gcgtacagcg cactctggcg cggctgaccg
     7981 cagggtgcgc gcagcagacg cagcgcagca ggtccgctgt atgaggcagc agaattgaac
     8041 tgggtgaaca ggatatcgaa gcgtttccac agcggatgat ggtcgagacc ggcagcatgc
     8101 acgtcgttcc atgacagcga gcagacgtgg atgaaaacga tgtcaaatgc cggtgccgct
     8161 gccgacggca gggttacctt gcgtgtggct tccgtggcat agaagctgtc gagaatgtta
     8221 tcaagcgggg ccgtggtgct gcctgaagcg gcgatgttcg agccggcgtg tgcgctggcc
     8281 tgcactgcga tgccatcctt gccgttacag ttctgcgcgc tggccggtag tgttggccgt
     8341 aacgattggg tgaaagcgag cgcggccatg atcccaacga ggatgacgcc gacacgcaac
     8401 cgctgcgtga tgaaatacca cgcgccccac aggatgacca atatcgcaat gtcgcgcgcc
     8461 cggatgaaac gtgcaccgag ttcggtcaga taggacaggt tgaatgataa caattccggc
     8521 aggtttgcca tgatgtgtgc cggtggcggc agccaggaat cgtggtacag caaggcaatc
     8581 gctacaggcc aggcaagcag tccgcgcaca cggcgccagc gcggcaagga cagcggtgcg
     8641 agcaggaaga cggcgaacgc caggttttcc agaggatgga attgtgtcag tcccctggca
     8701 tagaaaaaca gtgcagtaag gaaatagaat tcccatccac ccaggctgac gggtggcctg
     8761 ggcgtgcgca cggtaatggg aagatcgatc gaatcaggat gttctggcat gtgggtcaac
     8821 cggggctgca gtggcggcgg gtgtggacgg gtgcgcgggt gggtgataag ggtgcagcag
     8881 ggcggtacca gccgatgtgc ggcggcgcag cgcatcgcgc tggcgagaca gacaccagcg
     8941 gtagccgaaa ccggctccgg caatgaaggc aaccagcaag gacagccagg acatcatggg
     9001 cgcacccccg gaaaagaccg caatggcagc gggcggcgga tggctgcgcg tggctgacgc
     9061 atccgtaccg gaggctgggc gggtgcatcc ggctgcggcc cgggtggacg gattgctacg
     9121 ccaccaccca cttccgtgcc ggtccgggac agtgcgtcga gcacatcacg catgctgttt
     9181 tcgtcgaaca gacgggtttc actggcaaac agatcggtgc gcggtgcgag gaacaactgg
     9241 tcgagcgtgg catcgagatc gctttcgcga caggcaaaca aaaatacgta caattggtgg
     9301 ccatcgtcag tgcacaggtc gccacgccgc ttgagtgcgc attgacgcac gatttcggcg
     9361 agcgtcatgt cggcggttgg cttgaagcgc accagggcat tatggatggc aagcgtgcgt
     9421 gagcgcagca gcgtttctcc ggcttcacgg gcaaactcgc ctgcgtcgag ataacccggg
     9481 gggcgcgccg gcaggaagtc caccggcagt tcaccgcttg cggagacggg tttgcgtacc
     9541 tgctgtgcca gcatgccgac cagcgattcg atccaggaga aaccggcttc tgccggaacg
     9601 atgatgtcgg cgccggcgtg ttgcagcagg cgctgttgcg catgacggac gtggacgttg
     9661 atttcgcgca cgaggatgcg tacgccattg ccgagatcgc tcctcagttc gagaatggtg
     9721 tgggccaggg cctgggtgtc ggaatcacgg tcgaggtgca ggatcagggt tgcattgtga
     9781 gcgccgcgtg aagcttcaac cagcgctggc aggtcctcgg cgacctgcca gcctgcgggt
     9841 ggggtgcgtg ttccagtcag gcttgtgcgg gtgagaaaca cttcttgcgc ctctgtagcc
     9901 agtggcggta cgcctgcgac gggtggccgg acggcctcgg catgccagcc gccatcgggg
     9961 cgaggctgga ggtggaacag ctgtcggcct tcaatgccgt gcgagccgaa ccagtgcagg
    10021 gtctcccagc tggtcgttgc gctgtcatgc gacaggcgtg caagtccggc aagatggttg
    10081 gcgagcggca gcagttcgcg tgcaggatca ggatgtccgt cgcggtgcgg gtaaagcagc
    10141 aacagcgtac ggccgctggt atcggcttca tgccggcact ggcgcaggaa tgcggatgaa
    10201 acggcgggaa ccttgctgcc ggaaaacgcg ccggcatcgt cgatcaccac cagtgcatgg
    10261 ctgccgacgc cgtcgcgggc cagttcgtcg ttcataatgg cgcagacatc cggcatggat
    10321 actgcgtcgt ggcggtagat gcgcagcctt ccgctgtcga gcaggtgccg cgcgtgtctg
    10381 tccagcagca gtggcccgaa cacgtgtgcc ggatctgcgt gcgtgatcca tacgccctgg
    10441 ccgtgttccg cggctgcaag cagcgtgcgc cagagcactg tattgagctg gggcgggcca
    10501 tcatgcgcaa tggcgtacag gcagccagag accagtgacc cggtttcggc cggcaggttg
    10561 cccagttgca ggcggcaggg cgggatcatg gcaactgtcc ctgtccggag gcaggttggg
    10621 gcgcgggttg ggcacgcgtc agctcaggcg cgcgggcggt tgcagaagag gattgtgcag
    10681 cgctggtcat tttgcccgtc cagaggtcat ggtgtgccgg cagtgcaggc ctgcaaggaa
    10741 attgtagcat ctggccgtgt tccgcgtatc ggtcacggcg atggagctga catggtttca
    10801 tggcgcgcgt ttcttgctgc gttgtgccag gatttcgcgt actgcctgca aattgcgagc
    10861 gatacgcgta tcgcgcgggg ccagacgtgc ggcgcgttcg agcagaatgc tggcggtggc
    10921 atcgtcaccg cgttgcatgc agcgtatcgc ctgttcgttg agccggcccg gattgacgtg
    10981 gacatcggca gcggttgcag acggggtgcc gccgctggcc agtccggtgc acagcagtag
    11041 ggtcagcgtg agtgatatgc ggaggaagat cggcatggcg gcggcctcag aagctcgaag
    11101 tcggcaggac aggattggga ggcaacgcca ctggctgggc tgccgcagcg ccgaccgcat
    11161 agcgcaggta cagcaggaag gtgttgggtg catagtatgg ggaacgctcg atttcgagcc
    11221 ggctgcccac gaacagtcgc gaggttgcct ggtactccga agcggcgctg aacgaatagc
    11281 cgctaccggg accactgctc gtgctgtacc acggattgcc tgccgaggcc tgcaactgcg
    11341 ggtcggtcgg gaaatagggc gaggcgtcag tctgtgacca ggaggtgaac accgaagcgc
    11401 gtatggccca ggaccagcgg gcatagcgtt gcgcccaggt gaccggcagt gccagcgaac
    11461 ggtaggtctg cgggctgtag tagccaccct gaccgaaggt gaattcgccg gagtcctgtg
    11521 tgtcatgcca gtccattgcg gtcagtccga gagaaaacag ccggtcttcc tcgttgacgc
    11581 tgcgacaggt ttcgccggca atcgccagca cgcgctggtt gctctgtaca ttggtgccgg
    11641 tgagttcgtg tacgccgaag cttgaccaga acccgagcgt accgccgttg tcgagactca
    11701 atccggcgcg taccccggtg gcgagtacgc cgccccagac caggccagta cgtggatcgg
    11761 tggtgccggc aaatgacaac agcgtgctgg tgtcctggcg gcgcgaggca tcaagcgacc
    11821 aggaaaacgg ccctgcatcg cctttccagc gcagtccgcc gaccagattc cacaccggga
    11881 acccgagtgg cgtggtgccg aaatcggcac gcagatggtc attgacgtag ccggcggtca
    11941 gggatgcgcc ctgtgcctgc tggctggcct ggcccgcgca gctggcgttt gacagcagca
    12001 gcgtgccgaa agtgcgtgtg gcatacgatc cgctgcagtc gagcgtgccg gcctgcaacc
    12061 gcaccagatc gctacgtgca aaccactggc caccccctga ccagggggct ttccactcga
    12121 acggcagttg ccaggcttcg aattgtgatt gtcctggagt gccggcgcgg gtttcccagt
    12181 cgagcgccga ggaaatccac gggatatcct gatcgagcat gctggccagc cgtcggtact
    12241 ggtaatcgcc gccggccgaa tccgtcgctg ccgggtcggc agctggcgtg acgctgaagc
    12301 tgccgtcgcc agtcgacgca ggacgcagcg cggacagggt gcgtgagtcg tcgatggctg
    12361 gctgttgcag cgccaacgca tgctgcaatg tgccgatggc cagcccggtc tggccacgcg
    12421 cataggcgat gcggctggct gcatcgagtg cgcgggcgtt gtcgggttcg ccggccagca
    12481 cggcgtcgac atactgccgg gcgacggtcc aggcatgctg gtcaatcagc cagttggcca
    12541 ggttgaggcg tgcccatgca ggcgctggtt gtccttgcga gatccattgt gctgccaggc
    12601 ggatcatctg ctggcgttga tgctgcgcgg ccaacagcgt gagccattcg atgcgggcgt
    12661 cgctggcatc gggatcgcgt gcgaccaggt cacggcacag ggcgatggct gcggtgaaat
    12721 ttcgctcggc agcgaacgtg cgcgccatgg caagctgcaa ggcaggatcg tccggccgtt
    12781 gtgccaactg ccgggcaagc gtgcgctgtg cgacatcggt attgccggca ccagtctgcg
    12841 cctcggcgct gcgtatgacc gcggcattgt gccgggcggc cgattcatct tcgtggtgct
    12901 ggcgctcgtt gcgcagtgcc acctgtagcg cgcgctgata gtcgtccagt ttggccgtgc
    12961 gttgcgccgg ttcgatctgt tccatgcagc tgagcgcggc ggcggtatcg ccctggctgg
    13021 cgagaaacag cgcctgggcg taatgcgcgt cggcggcggt tggctggcgt ttcagcaggg
    13081 catcgaacaa tgcacggccg cgcgcggctt cgccctgctg agcatgccgg cgcgccagat
    13141 cgaggcagat ccagggatta tccggatcga gccgggaggc gtgttcgagc agtgccaccg
    13201 cttcgtgttg gtgctcggga ccaagacggt ctgcctcatc gcgatagagt gcggccagac
    13261 cggtttgcgc gttgtgctgt tgcgggttgg cggcaagcgc ctgctggtag gactgtgctg
    13321 caccgtcgat atcgccgctt gcagcgaggg cggcagccag cgcgaccaga gcatcaggct
    13381 ggcccggttg catcgccagc gcggcgcgcg cctcgcgggc agccagcgcg gcctggccgg
    13441 catcgcgtgc gcgaccggat gcctgcatat gtccccagaa gcgtgccgtg gcgagcagac
    13501 tgttccattt gcgacgctgg ctggcatcgc tgcgcgcggc gcggcgaaaa cagtctgctg
    13561 cttcggcata gcgtgcctga cgcaaccgta ccatacccag gccgccgatg gcctgcatgt
    13621 cgtccgggcg cagtgcgagg gcgtgtgcaa attcgcgttc tgcgcctggc agatcgttgg
    13681 cctcgagtcg ctgctgtccg gccgcaatgg cgagtgtggc gggatcgagc tgctgctggc
    13741 gctgcgcgtt catggcggac agtgtctggt tcaacgctgt gcgcacgccc tcatcctgag
    13801 ggtcgaatgc ctgatactgg cgcagctgcg caatcacctg gcgcgactgc gcactgcccg
    13861 atgtggacgg ggcctggcgc agcacagccg aacgccatgc ctgttgcgct gcgcggccat
    13921 aatcagggtc acggctcagt gcggcgagtt gctgtactgc gagcggatcg accggcgcgt
    13981 gggcggtgcg cagttcggcc caggccagac ggatgcgcag gctgtccgga tggtcgcgca
    14041 gcagctgggt caacccttga tctgcggcct gccagccatc gggggtgcgc gccacccatt
    14101 gccagtattc gagcgccagg tcgctgtcgg gtgggccatt cgggcacagt gctcgtgccg
    14161 catccactgc ggcctgcggg tgtccgccac gcgccagcag acgtgcgcgt tgccagcgtg
    14221 cgcgatcggt ggtggcaaga cgcaatatgt catggatacg cgtgatcgct gggtcgtcgg
    14281 gtcgtacggc atgcagtccg gccagcgtgg ccagtgtgcc ggggcggtcg ccagcctggg
    14341 cctgcaatgt gcccaacagg atcagtccct ggagattgcg cggcgagatc ctgaacagtt
    14401 gccgcagctg attacgggcg cgttccggat ggtcgcgatc catccagaag cgcgcgcgtt
    14461 cgagcaacgc ggtctgcggg tcgtgcatga gcggcgcggc ggttaccggc gcagcctgca
    14521 ggactgggtc ggcagcactg gtggtcgctg aatggcgttg cggcaccgtt gaggcgttcg
    14581 tgcgcacgat acggaataca acctgaccgt ttgcgaaatc ctgcggcggc agcagggcac
    14641 gaacccgtgt gcggccatgg gcgcggtaaa atccggtgag ccggtcggcc agttgttcga
    14701 gctgggcaca ggtgaggcgt ttgccgcggg catcggcgat ctgccttgtc agctcttcgg
    14761 ctggaacggc cttcgagccg gtgatgcgga ttgattgcac cagcacgcta tcgttgccgg
    14821 gcgtgggcac ggccaggcac tggccggtcg cactttcgaa cgccagcccg gccaacagcg
    14881 tcagtgccct gcggcgcagg tcatgccggc atcgcatcgg tcgctattcc tccagcctgc
    14941 ggcgcgcaat accggccagt gctttgcgca gcgaagcggc ggcagtcagg attgtcagca
    15001 gggcgaagat gaccagcagt acggggtggt tcgatagcca gagccagagc cgcacccacc
    15061 acggcagctg gccggtggtg tagagctggc cggccaggta gctgtcgatg cggtcggtgt
    15121 ggaaaaaagc ctcgctgccc tgaatggcct gctgttgtcc tggtgtgtcc agggcattga
    15181 ggatcagacc gagctggtcg ggggaggatg cgaccagcgc aatgacactg cggtgaggtg
    15241 tgagcggcga ttcgaaccct gtcagcagcg ccagcgggcc attggcggca agccgcacct
    15301 gtgactgcgg cgcgggatca gggcgggcgc cgagttcgaa ccagtcgtag gcgtcattgg
    15361 gttcctggcg gggctggctg atgcgccgtg cggatgtgga caggttggcg ggaagatggc
    15421 tgttccagcg cgccagcagc ccgtcgtgca gcgatgaccc gatgacgatc agatcggcat
    15481 cggccagcgc cagtgtgtca tccgggccgg tcagtctgaa gcgggtggcg gggtaaccgg
    15541 tcgaggccga caggtgggcg agcaggtcga gcatggcctc gatgtgcgct gctgccggat
    15601 gtgtcggcag cacgatgatg gtctgcgaca gatcggcata gcgggtgaat ggaaagccga
    15661 ggctggcgaa atagttcagg ttgggcagct cggtatagtg caggaagccg gtgaaatcga
    15721 tggtcgaatc ggcatcgact gcagaacgtg tgccacccgg ctgagtgtct tcacacagtc
    15781 ccctgcgatg gtaggaaaac gcgaactgca gccggttttt cgtgccgagt ttgtaggccg
    15841 gcagaaacag cgagcggcca tcgtcgaaca gtccgtgatc gttgagggcc agccgtaccg
    15901 gatgctgcac tctgccgtcg gatgacttga ggttgaacgc ctggacgaac tggtcgttgg
    15961 cgctgatggt aagacgcgat tcgtcatcgt ggccaaccgg ggtgaagcgg aacctgaggt
    16021 tgagcggaat accccggctg tgccatgcaa acaggtctgg tggcatgcgc aatggaacgt
    16081 tcactggcgc gggttgcagg ccgctgcgtt ccagatcctg cggcgaatcg accagttcac
    16141 cgaatttcat cggtcgatcc atacgtaccc agcgtggagc atcatacgcg acacgtgggc
    16201 cggtatccgt cacggaggtg atgctggccc gggtgccgga cagtgcggcg tgaccaagcg
    16261 ccagcgccag cgctgccgtt gtcagctcct ggccattgcg ccccagcacc agcagcagcg
    16321 gggaatagcc atcggctgga ttgatcattt ccgccagcat cgggccgtcg atgcgtagcg
    16381 tcgggtcgag ccaggcaggg cgggtggcct gagtggcgaa cacgatggcg tggccgggtt
    16441 gcggcaggcc gccgacatgg acaggaaagc gcgcaccacg ccagtcggcg agcatgccga
    16501 accaggacgc gacaatgccg gctgcccgca gcgtatcctg gtccggcgtg gcggcaaaag
    16561 tgacgggcag caccaggcga ccggcgttgc cacggttgaa gaagggctcc ggcaacagac
    16621 cgagatcggg gcggaggggc aggctgcgca gttgcagttc aagttcgctg ttgccatcga
    16681 tggcagccca caggctggag ctttgcggat cttcgcaatc gctggcgatg tggccgacga
    16741 actcgaatgc caggcggttg aattccacga gcaggcgcgg atcgacggtg atgtcgctgg
    16801 acagcagttg tccggcgccc tgacgggtta ccggtagcac gccgatcagt tcatcgttga
    16861 gcaggacctt gatgtgtgac cgttccggaa cgagtgatgg cgaatagctg tagcgcaggt
    16921 gcagcgtagc ctgactgacc atttggtcgt tgctcaggcc gaaatgcact tcgaccacgc
    16981 cgtcggtccc gcgcagtgac aaccgccctg gctggccggt gatctgctgt aatggcaggc
    17041 gtaccatttg catgccttgt tcggactgca cgggtggcgc catctgggta tccggcgcgg
    17101 cgacgggaag tgcggccagt gcacaggcag gcagccagat cagcagcaga agcaggttac
    17161 gcgatagaag tttcatggtc ggtattcaac ggtggaagag gctggcgtgg cagccaccag
    17221 gaaatgcgct gcaggctgcc ggcgtggcgg atgcccagcg aatcgaaacg ggacagcagg
    17281 tgaatgccga gccggcggta gcccgacacc ccggaggaca caaagccaaa caggttgaac
    17341 agcggccggt cagcgggacg acctcgcgtc cagtcgatcc atgcatcggc acgactgaag
    17401 gtgcactgca ccagcgcact ttcctgctgc gtgccggtca gttgccagtg caggcgcaac
    17461 cgggtggcgc tggtagcgat gacacgggca gggaacacat attcctcggt accgcgccag
    17521 agcgagacgg cgacagtgtc cccggtacgg atggctgttc cggttgcggg cagcagtgcg
    17581 gcccccccca tggacacgtc ttccgtggtg cagcgcacga gccggccgtc cggcaggtgc
    17641 aggatggccg gcagttccat gcgtacccgg tgcgagatgc gcagttgccg tgcctccgag
    17701 gcgacgaaaa tggcgccgcc aaggatcagc aggttgtaga acgtccagcc catattgagc
    17761 cagacggtgg cgatttcttc cggcggtccg aacacgctgc gcgctatgcc tgccgccagt
    17821 ccgatgccgt tgagcgcgat caggctgaga aatggggtgg agatggtcag gtcgaaaaac
    17881 tcgtgttcga ccagtccgcc cttggcggtg acgttgaacg cactgccgcg cgggttgaac
    17941 agggcggcgg ccgtaggtcg cacgatgtac cacgacagca cggtttcgta cagttcaccc
    18001 cagaacgaat agcggtacct gccctggatg tgcgagttgg tcatcgagcc aacccccatg
    18061 tgcaacaggg cataggccag taccatgacc ggcggggcat aaatgatgta ggagtgaaac
    18121 agcaggaagg caagcggtga cgtgaggaaa accagccgcg ggatgccgcc gaaaaagtgc
    18181 atcatggcat tgccataaca gatgcgctgg ccccagctca gtcctttgcc gaaaaacggg
    18241 ttgtcgaagc ggaaaatctg cgccatgccg cgtgaccagc ggatgcgctg gccaatatgg
    18301 gccgacaggg tttcggtggc cagcccggca gcctgcggaa tcttgatgta ggcggaacga
    18361 tagcctgcac ggtgcagcct gagcgccgtg tgggcatctt cggtgacggt ttccacagca
    18421 acaccgccaa cttccagcag gggctcgcgg cgcagcacgg cacatgaccc gcagaagaag
    18481 gccgcattcc agaaatcgtt gccatcctgg atcagtccgt agaacagctc gccctcgttg
    18541 ggcactacgc ggaaggtgcc cagattgcgc tcgaatgggt cggcagaata aaaatggtgc
    18601 ggcgtttgta ccagcgcaag cttgcggtcg cgcaggaacc agcccatgct ggtctgcagg
    18661 aaagagcgtg tcgggatgtg gtcgcagtcg aaaatggcga cgaattcgcc gtgtgaatgc
    18721 gccagtgcgt ggttgaggtt tccggccttg gcatgacggt tgtccggccg ggtcaggtaa
    18781 tgtacgccga ccgatccggc gaacagacgg tattcctcgc gacggccatc atcgagcaca
    18841 tagatgttca gccggtcggc cgggtagtcg atttccctgg ccgcgaggat ggtttttctt
    18901 accagatcca gcggttcatt gtaggtgggg atgaaaatat ccacggcagg ccacagcgac
    18961 gtgtcctgcg gcaatgagac ggccgggcgc tgcagtggcc aggcggtctg tacgtaaccg
    19021 aacaccagga tgagccaggt atggagctcg gcgccgagca gcagcagtcc ccacaaccag
    19081 tcgagtggct ggtcccagtt cagcgtggtg ctgaaacgcc accacaggta acgtgaggag
    19141 actacgatgg acagcgaaat cagcagcagt gtggccagcg gtccctgaat ggactggatg
    19201 acgaatacca gtgcaaaaat cagtgtggca aacaccagtt gcgacacggt gtcgagcgga
    19261 gtggtcacgc acagcagcgc gagcacgccc gcgatgcccc agacgagata gcgcaccggt
    19321 gcccgttgcc acagcgacag gtcggcaaac cgggtgatcc aggtggatgc ccggttgcca
    19381 tattgcgcca ttgcggcctg cgctggtccg tgcgccagtg cgggcagcgc cagtcgtggg
    19441 cgcagccagg cgggccagcg tgccggatcg taccagcggg agccgcgccg tagtaccggt
    19501 gcgggacggg caggtcgcac aagtgccagc cacagcagtt gcaacggaat acgcagaaga
    19561 tcgccaggat gcggctggtc gaagcgaatg tggggaaagt ggcgcgcaag ccagtggcgc
    19621 cagggtggtg taccgttcgg ctggcgcagc aacagcgaag cgatgcccat gcgccagccg
    19681 tgcgcggtgt cgataccgta gcgggtgagc agccagcgca gacgggtgcg ccagtctacg
    19741 ggtgcggcgc tcatgccgtg gcgctctgcg gtgcggctgt cagccagaat gccagtcgtg
    19801 tcaggtcgct ggcggcctgg ctggacggtg catagtcgaa cacggtctgt ttgctggcca
    19861 gcgcctcgcg cagcgattca tcgcgatgca cgaccaccgg cgccagcgca ttgcccagtt
    19921 gcgcgcgcaa caggtagcgc acgtcgtggt ccagatcacg cgatgcatcg tagccgttga
    19981 gcacgaatcg cagtgatgcg ttggtatccg ggcgcaggct gtcggcaatc cgtcccgcat
    20041 tggaactgga catggcgtca ggtgcgagca cggcgacgcg gatggtcgag gcggcaacga
    20101 tctgggcgcg cagcggggtg aacggaaagg gtacgtcgtt gatgatccag gcatcgtcag
    20161 ggatgtcgag tgtggccaga cagtcgctga accagcgtgc ggcacgtggc ggatcgaaca
    20221 gcgcttcgaa ctgctggcgg gcgctgtcgt gttccagcgt gccataaggc atgaagtcga
    20281 tgccgtccgg gctgcgccac gcggcttcgt tccagggctt gcctgccagc agttgcggtg
    20341 ccagaccgtc cgtgacgttc cagtccatgc cgaagtgcag ccgcagggtg ttgaaggggg
    20401 aaaaatcgaa cgccagcgtg cgatggccct gctgaccgat gctgactgcc aggtgagcgg
    20461 ccagcatcgt ggcgccggcg ccaccgctgg caccggtgat gctgatcacg ttcattgctg
    20521 ccgctccacc gaaccggccg cgtgaatttc gcgcaacagc ggccagcgca aaaaacctcg
    20581 ggcggcagct tcctgggccc ggatttcctg gtagacgatc tcgcggccgc ctgatctggc
    20641 gagcaggtga acgacatcgg ttggaggtac tggctctgtc actggtttgt gttccggtca
    20701 aaacccggtc ggccagccgg atgggctccg tgcaccgggc ggagtcgcta actcactgtt
    20761 gtcaaagcat ggtcactctg ctgcaaccgg tgcgccaggg cgtgttccag gctgcccggc
    20821 aatggattga agattcgacc atctgctttc tttactaaca atcatgcatg ggtcgctggc
    20881 gtgctgtcaa gatagaatcg cgtattttcg taagttaatg taggaagttt atggattatg
    20941 tggtgagaat acggcagtgc cagtattgga tgcagtgtgt gccagcaacg caagcaacgt
    21001 gaggctgtcc tgataatagt ccctgccttc aatgacctgg gtaatcggca gggcgcgtgc
    21061 cagttgcaga atggcgagct gaccgccgcc ggccgagtag ggcgcggcct gattgttgcg
    21121 cagatcgaaa taggcgggca acaccggata atggtcagca tagcgaagaa aacggtgcac
    21181 aaccggatga tcggcatggc ccgaccagat gagccaaagc gggatgcgga tggcatcgta
    21241 gccgaagcgg gcgtcgaatg cagccgccgg ttgcaccgga tcatcgagtt gcagccagtc
    21301 aggtggcagt cgccagtcgc cgaaataact gtaatcgagc aggcgcaagc cggtattggc
    21361 gagatggttc cacggtgcat ccggcaacag tcgggaaaaa agtggcaagg ccggcaggag
    21421 ccagtaagac aggttgacga ccggggcatg accgggccgg gtgaagcctt gtcgaccggg
    21481 cagcaggatc gtgccgtgcg ggtcgggacg gatcaggtgc cgggttaccg cgcgcagggt
    21541 gttgcgtgcc tcttcggtgc atgagtcgtc ggcaaagttc gtgccgccca gcgtcagcgc
    21601 ccaggcgata tagagatcgc catccgctgc gtcattgctg tcggcaatgc cctgcgcgct
    21661 ccacagccaa ctgaacaggt cgtcggtgcg gcgcaggcgg gtggtgaagc ggcgtgccgc
    21721 catgaacgtg ttgcggtcgc gcattgccgc ggcgaacagc atgagcaaac cctgaccttc
    21781 actgtgtgaa cggttgtgca aatgttcgtc gaacacctgg ccgttgcgtg tatgcagaga
    21841 gacaaatgcg tggtagctgg cggtcagtgt actcggggcc ggtgacctta gggcagcagc
    21901 ggcgctgcgg gatgcacgca gagcgagcgg cgccgtgaga ctgtgccgga gaaactggcg
    21961 tcggttcatg tgcacagggg gcggcacccg tcaggccgga gtgcgcgggc ggaaccgcgg
    22021 ccagtcgaaa tgtggcccgg gatcggtttt tcgtccgggt gcgatttcgc tgtggccagc
    22081 cagtgcgacg ttcggccagc gctcgcgaat ggcggccaac agtctttcca gtttgcgata
    22141 ctggcgctcg tcgaacggct gatcgtcgca gccttcgagt tcgatgccga tggaaaagtc
    22201 attgcagcgc tgccagccgc accatgagga tgggccgcaa tgccaggcgc gcagcgagca
    22261 cgggacgaat tgcacggttt caccatcgcg gcggatgagg aagtgcgccg agacacgcaa
    22321 tccttccagt gcggcgtaat ctttccgcag cgaggtgtcg agcgtaccga ggaacagccg
    22381 ttcagcatct gcgctgccaa aggtgccggc tggcagtgat atgttgtgaa tgaccacaag
    22441 ccggatgtca tccggcaggg ggcgcgcatc gcagtgtggt gaagccgcaa gacggcagtc
    22501 gcttgccacg ccttgtgcgt cgaggtgcat ggacgctcag ccgagtgcgg ccagtacccg
    22561 ctgcgcggcg ccgggctgat ggctcagcca ttcctgagcg cggcgcgtca gcgcgctggt
    22621 ggcgtccggc gtgtcgatga gctggcgcat gtgggcgaac acagcctcct cgtcattgcc
    22681 ggcaaggacc atgcctgctt cgagtgctgc ggccagcaat ggctggccgc ggccggccgg
    22741 gccgaccagg atcggccgcc catgctgcat gggtgtgatc aggtcaggct gggtctgggc
    22801 attggcatgc agcgtgccgc cggccaccac gaaatcggcg caggcgtaca ggtcggcaag
    22861 cggttgggca tcttctacgt aatacacacg tgtcttgatc ggcacgtacg aggtcgacaa
    22921 acgccggtgg cggatggttt gcaggcggta tctgagcgag tcgcggtata ccggttcgca
    22981 gcgtaccggg tcgcgtggcg ccagcagcat catccccatt ttgtaacgga tggcccggtt
    23041 gaacagctgg aaggcgattg cttcctcgtc ttcacccgtg ccggcgaaat aacccagcca
    23101 gcgattacct tcgcgctgtt ccttgaagcg ctggcagatg ccggtgttga cactgagcgc
    23161 aggcaactgg gaaaccgccg ctagcgggtc gcccgtgacc gccgcagacg gcagttgcgg
    23221 taacggatgc gagacggtga cggccttgaa gcccgccttg ccgatcgcct cgtcgcgtgc
    23281 gttgatccag aataccgggc agccggccgc ctgtgcggtc tcgatgtggc gttcgtccag
    23341 gccgagcacg atcaaccgct gtggttgcag cttgcgtagc cggacgccaa tgtcttctgt
    23401 ttggccggac aacgtcagtg acggatagcc gtacgcgacg ctgccgccgc cgtccagtac
    23461 ggccagtgac aggttgccaa agcgttgatg taactgttgc aggaaagcgg aagcgcgttc
    23521 aagcgtgttg gcttgcgcaa tgacgatcga gctgccagcc atgccggcag gcgcgcgatt
    23581 ggagcggaac agatccagcc agcccatggt gtggtccttg atgcgatggg ttcaagccgg
    23641 caagcatacc ggaaatcatg cgtggaaaaa accggcccgt gatgatcggg ccggtgtcga
    23701 ggggggatca gaaatcgttt tcgaccttga gggcattgag cagcatgtcg gcggccagca
    23761 tgttggccgc atggttcaga ccgtcgagaa tgtcggcatc actccagccg gccttgcgca
    23821 gtgcgtcgat gtccgttgcg gtgaccgaat tcgagtcgcg tgcagccttg agggtaaaca
    23881 gcagcatggc cacatccttg ggtggcagcg gtgcgctggc cgggtcgcgc ttggccgcct
    23941 gcacctgttc gatactgaaa cccgcatggt tgatgagcag gctttcattg aagcccacgc
    24001 aatattcaca gtgcccctgt tgcgacacca gcatgcgcat catggcgagc agagcaaaat
    24061 tcagcgtcgg gtgttgcatg taatagccga ttgactgcca atgctcggcc agcagcgcgg
    24121 ggcttgcgct gtagatctgc agtgcattcg gaggcgcgcc aaaggcggcg ctgacctgat
    24181 cgtagacttc cttgactttt ccttgcgcgt cttgcgggga aacggtttga atcaaaggca
    24241 tggcactctc ctcaggttgg ataccgacca ttcggtatcc agtggcagca aaacggtgct
    24301 gcgtccgtgt agcaaacggt tcaggccctg tgcgcgtcgg gcatgagacc gacgatatag
    24361 tggcgcagtt gaccgacgca taccttgaag gcggcggtgg actgcatgct cttggcgacg
    24421 ccggtgcaac cttcccaggc cgaaagtacg aacagcgcgg cggcctctgc atcgacgtct
    24481 gcgcgtaccg tgccctgctg ctggccggct tcgaacgcct gacgcagcac agtgcgccat
    24541 tcggcgagta tccggttgag atgtgcgcgg aaggtttcgt ccagcgggct catttcctgc
    24601 atcaggttgt tgagcgggca gcctagcagc aggcggtcgg cctcgagtac gtcgggcagg
    24661 tgcagcagaa cctcgagcag cgcctggacc ggattggcgc tgctgcggat cggttcgagt
    24721 accaggcggt cgattgtacc ggcgatgact tcatcgatta ccgccagtcc aagtgcatgc
    24781 ttggtgggaa aatggtggta caacgcgccc ttggtcagtc cggtcgaatc gaggatgtcg
    24841 gccacgcttg ctccctggaa tccgttgcgg tgaatttcgc agaatgcaga ctgcagcagt
    24901 cgctcgcgtg tctggtcggt ggcgcgtatc aatgtctcgg tgttcatgcc tgtattacat
    24961 accgaccggt atgtatttgt caagcgccgc cggcaaatcc gttctgacgc caggcttcgt
    25021 agaccacgat ggcgacgctg ttggacaggt tgaggctgcg gcactccggc cgcattggca
    25081 gacggatgcg caggtcaggg gcgaactgtt caagaatgtc tgcgggcaga cctctggttt
    25141 cggcgccgaa cacgaacacg tcgccgggct ggaaacgggc ctgtgcaaac ggggtgtcgg
    25201 ctttgctgct cagcgcgaac atgcgccctc cctgcaaatg ctgcagacag gcagaccagt
    25261 cggtatgggt ttggacacgg acgtattcgt ggtaatctag cccagcgcgc cgcagttggc
    25321 ggtcgtccat gctgaagccg agcggttcga tcaggtgcag gcagcagccc gcgttggcgc
    25381 acagacgaat gacattaccg gtgttcggag gaatctccgg ctggtacaga acgatatgaa
    25441 acataggcgg gatactgcga ctgagatcac ggattctgcc agattataac caggaaaatc
    25501 gcatggttcc tcatttgact actgcgctta caggtccgtt gcttgatctg gaacgccacg
    25561 tgctggccgc cacgccggcg atcgagcgct ggtttcgcag ccagtggcat gaacatgaag
    25621 ccccgttcta cacttcggtg gacttgcgca acagcggatt caagctcgcg ccggtggata
    25681 ccaacctgtt cccgggtggc ttcaataacc tgaatatgga ttttgttccg ctcgcggtgc
    25741 aggctgccat gagcgccgtg gaaaaaatct gcccggatac gcagcggctg ttgctgatac
    25801 cagaaaacca tacccgcaat atcttctacc tgcaaaatgt ggcgcggctg gcggccatcc
    25861 tgcgccagac cgggcttgag gtgcgcatcg gcagcctgat tcccgatctc gaacagccgc
    25921 tgccgatcga cctgggcgat ggcgaaacgc tgctgctcga accggtgatc cgccgaggcg
    25981 cgcgcatcgg cctggatggt tatgatccct gtgcggtact gctcaataac gacctgtccg
    26041 ccggggtgcc ggagattctg cgcgacctgg agcagcgcgt gttgccgccg ttgcgcgccg
    26101 gctggcatgc gcggagaaag tcgcatcatt tcgccgccta tgagcgggtg gcggcggatt
    26161 tttcccgatt ggtcgacatc gatccctggc tgatcaatcc ctatttttcg gtgtgtgaaa
    26221 aaattgattt tcaggcgcgt caagggcagg aatgtctgga agagcaggtg gcagacctgt
    26281 tgcggcgcat cggggaaaaa tatcgcgaaa accgcatcga cgcgcaaccg ttcgttatcg
    26341 tcaaggcgga tgccggcacc tatggcatgg gcatcatgac ggtcaaggac gccaccgagg
    26401 tgcgcgagct caatcgcaag caacgcaaca agatggcggt gggcaaggaa ggcatgcagg
    26461 tcagtgaggt gatgatacag gagggcgtgt acaccttcga gagcatcaac gaagcggtgg
    26521 ccgagccggt ggtgtacatg attgatcatt tcgtggtggg cggtttctac cgtgtgcata
    26581 ccgggcgggg ggtggacgaa aacctcaatg cgccgggcat gcatttcgtg ccgctggcgt
    26641 tcgagacacc gtgctgcatg ccggatcgtc aggccaaccc ggattccaca cccaaccgct
    26701 tctataccta tggcgtggtt gcccgtctgg caatgctggc ggcatcgctg gaactcgaag
    26761 ccgatgccgg agggacggca tgaacctgct gctgttgctc gatccgctga acacgctcaa
    26821 gccttacaag gacagcagtg tggcgatcat gcgcgcggcg gcgcggcggc aacatgcggt
    26881 gcatgccgcc ggccccggcg atctgctatg gcgcgacggc gcagtgtggg cgcggacaca
    26941 agtgctgcgc gtctccgacg aggacgcgca ctggtacgat gcggccgagc cgaagctgca
    27001 gccgctggcc ggttttgatg ctgtgctcat gcgcaaggat ccgccgttcg acagcgaata
    27061 cctgtatgcc acacaactgt tgtcgctggc acaggcgcag ggggcgaagg tgttcaatgc
    27121 gccggatgcg ttgcgcgact tcaacgagaa actggcgatc ctgcgttttc cgcaatggat
    27181 tgcgcccacg ctcgtgagcc gcagcatgaa tgagctgaaa gctttcgcca gccagtatgg
    27241 cgacgtgatt ttcaagccgc tggacggaat gggtggcatg gaagtgtttc gcgtgcgcga
    27301 agacggactc aacctgaatg caattctgga aacgctgacc cgccaggaaa gtcgcaccat
    27361 catggctcag cgctatttgc cgcagatacg tgatggtgac aagcgtgtgc tgctgatcga
    27421 tggcgaggtc gtgccctggg cactggcgcg gatgccagcc gagggcgaga cgcgcgccaa
    27481 tcttgctgcc ggcggacgcg gcgtggcgca accactcgat gagcgcgagc gcgagattgc
    27541 cgaaggtctg gcgccgcagt tgcgtgcggc aggactgttg ctggtggggc tggacatgat
    27601 cggtggctgg ctgacggaaa tcaacgtcac cagcccga
//
LOCUS       DAIANL010000036         4886 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1057148, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000036 DAIANL010000000
VERSION     DAIANL010000036.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057148"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          854 a         1362 c         1615 g         1055 t
ORIGIN      
        1 tcgatcagcg ccttgatttc cttggcagcg gctgcgctgc gctgcgcgag gttacggact
       61 tcactggcga caacggcaaa cccccgcccc tgttcccccg ctcgcgcggc ctcgactgcg
      121 gcgttgagcg cgagtatgtt ggtctggaag gcgatgctgt cgatgacgct gatgatgtcg
      181 gcaatcttgc gcgaagagtc attgatttcg cccatggtgg caacgacgtt ggcgaccatg
      241 ctgccgccct tctcggccac gagggtggcg ttctgcgcca gtgtatcggc ttcgcgggcg
      301 tgatcggcat tctggcggat ggcggctgtg atctgctcga gtgcagaggc ggtttcttcg
      361 agtgagccgg cctgggattc ggtgcgtcgt gacaggtcga ggttgcccga gctgatctcg
      421 cctgaagcca aagccaccga cttggtgccc tgtcggactt cgctgaccag atgcgacaac
      481 tggctgttca tggttttcag cgcatcgagc agttcgccga attcgtcgct gctgtcggcc
      541 acgatgtggg tactcaggtc gccggcggca acgcagcgcg ccacttcgac ggcgcgaccc
      601 agcggacggg taatgctgcg tatcagatgc catgccagca cccctgccag gatgaccacc
      661 aatcccatga tgacgagact ccagatcagg acgcttctgc ggtcggccat gagctgctcc
      721 atgtgcatgt gcgtgctggc aaggatgctt gtgccgacat cttccagcgt gtgcgacggc
      781 gcccgatcca tgccggccac actgtcgtcg cctgctgtcg ggtcaaagcc ggcagcctgg
      841 aaggcggcga gtccgcgccg gtagttgttg cccatggtcg cgtgttcgcg catgaactgg
      901 tcgatgaggt ttgcggcgtc actgcccggc accaggctgg cctgcagtct ggccgcatcc
      961 tgcctgacat cggcctcttt cctggtgaag cctgcccagt atttttgcag tgcaaccgga
     1021 ttcttgccgc gcaacaaggt gtctttccag tcctggacct gaatcttgaa gtcgaccagc
     1081 atgcggttga cagtggtctc gttgccgtag tcgacctgaa tgattgtggt gtactcgttg
     1141 ttgagctggt tgaggcgata catgccgaac agaccgcctc cgatcgacag cagcaggctt
     1201 gcggcaaata tgacggggag cttgtgggcg attttcatgg cgtttccctt tttcaagaca
     1261 cgtgatgagc gtcagtccgg ttgtgccggt ggattggcgt tttttataat caaaagcgat
     1321 ctgttgtcat tcgtactttc gtactcgctt gtatctgact gatttgtcgt atcgaagaat
     1381 tcagtcagct ttttttcaat taaaacgaac cggttttcat ggtatgccag tattaaccgc
     1441 gtgtccagaa tgggattgaa aatgatgcgc atgagtgttc cagccagcgc cagtgcggca
     1501 ggcgtggtgc aactcgtgca gacagggaat gtcagcagtg tcgggcggga tattgcagtg
     1561 gcaaatgccg tcagcggcgg ggtggtcggt gagcggcgtg atgggatggc agttgttgca
     1621 acgcggtgct gaactgccgt tcggcttcct cgctgcggcc atcttcgatc agttgcggca
     1681 acaggccatc gagctgggtt tgcaggaaat ggctacgtaa aacaggatcg ggaatggcct
     1741 gtttgacggc atcgaggcgc tcggcaaaaa actgagccag acgatcgatg gtggcgggca
     1801 gactggatgc aatgcgtgca cgcagccagc gcgaaaatac cggaccggca ccggacgagg
     1861 cgatggcgat ggtgaccgga ccgcgttcga gcactgccgg cacgatgaca tcgccgcgtt
     1921 cgggatggtc ggcgacgttg gcaagaatgc cacgttcgcg tgcagcccgg gctacggcag
     1981 cattgaccgt ggcgtcattg gtggcgcaca ccgcgagcag gcaaccgtcg aggtgcgagg
     2041 ggcggaaggg ttcggcgaag tggaccacgt tctggccatc gacatgctgc gccaggctgg
     2101 cgtgcagctg cggagcaatg atgcgcaggc tggcgccggc cgaatgcagc cattcaaagc
     2161 gacgtgcagc gatggtgccg ccgccgacca gcgcgcacat ccgttgccgg acatcgagaa
     2221 acacgggcag gtggcgcatg gacgaacccc ttcgggcagg tggctgatac tcccgatttt
     2281 atccgttttc gtcgggcttt cgggaagagt ggaacacgac gggctggcgc gccatgctgt
     2341 caccagcata gtggggccgt tcgggttggt ccaggttggc cagttcctgt tcgcggatgg
     2401 caatcagctc gaaacagcgg cggatgtcct cgacagtgct gtcgctcagg cagctgggca
     2461 cgccggcgcg cggtgcagca tcgcgcacga cgttggacag taccttgcgc atgatgcgca
     2521 gcacgcgttg ttcaatcgaa ggctggtcgg tcatggggtc tccaggtgcg ggcagggcag
     2581 ctcaggtgcc gtggatgcgg tcatggtagc gtgcgcggta gatgagtcgc tgttgttccg
     2641 cgctgtcggt aaagccggtg cggtcatgca cgatgttgtt gctgatcagc cccatgccgg
     2701 cttcaagccg gccgcggaag atccacggca ggtcgctggc cagcaggcgc tcgaggctgg
     2761 cgacggcggc gcgtgtcgct tcatcgtctt tccatgcgat gctgcgtgtg cgtgcggtat
     2821 aacgcatgtg caggctgcca tcgggcaaca cggagaacac cgggccggtc tcggcggggc
     2881 gtgccacgcc gtgttcgtcc atgcgcgccg gaatggacat cgcatccggt tgcatcaggg
     2941 cggcgatggt ggcgggattt tcgtcgcgca gcagcaggta gacgatttcg tggtcgatca
     3001 gcgcattgct gccgccttcg gcggccggac tgacgcagtg cagtaacaat gcgcgtacgc
     3061 gccgggaagg tgcgttgtag tagccgtcag tgtgccagcg tatcggccgg tccgtgtagg
     3121 ggatgaattc gccctgcatc gtgcccgatt ccgccacagt gagcgaactg atggcgtcat
     3181 cgtcggccag catgttgtga tcgagccggt tcaggccgaa ctgcagtccc aggtcacgcg
     3241 gtacacgctt gtctgcgccg ggtggctgct tcagcatgta cagcgccaga ttggtcttgc
     3301 ggcagcgtgc aagcagcgca gcgtgttcat cggtgctcaa cgcatgcggg tcggcgattt
     3361 caaccagcag ctcgtcaagg ctgcgcggat agtcgcgcag tttgcattcg cgccacgccc
     3421 ggtaggaggt ttcgttatgc aacaggaaag gattgcctgg catggttacg atgcagccgg
     3481 ttctgcggtg gccggaaaca ggccgcgcac ggtttttgtc agggtttcaa gcatgactgg
     3541 cgcctcgaaa tccatgatct ggtcaatgat ccatgactgg tcatgtcgtt ccatccgttc
     3601 gagaatgtga ttgcccaggc aacgcagcac accgaaatcg ggctgcccgg gcgcgaagtc
     3661 gaagctggcg tattcgtcac agcgttcgtt gaccaggtcg atgaacgcgg cctgatagcc
     3721 gaattcgcgg tcattgagcg tttcatggat gttttcctcg acgatttccg cgaggcggcg
     3781 cgccatatgc tgcaccagcg tttgccgctc gttttcgctg atgtggccgt agaccatgcg
     3841 gtcgacctgg tgcagcatga acaccgacag ttccgccagc agcgtgaagc cgcgttgtgc
     3901 ggtcagaatg tcaaagtcgg cgcgggaaat gttggccacg gcctgatcgg cgagccgcca
     3961 gatggtgctg gcgattaccg tcgcctgttg ctccatgctc ttggggccgt cgatctgctt
     4021 gaaccaggtt gtcttgacgc gaatggccat ggtgatgatt ccgggtgtgt gccgcgcgga
     4081 gcaggagttc cgcgcgacgc ggggtacagt aacaaaccgg cagaccggtc agtagccgcc
     4141 cttgccgaac ggcttggtca ggccggctac gcgtgctgcc tggcgggcgg gctggttggg
     4201 gaacagggtg tacagatgtt tcttccagtc ctgatccgga tggagttctt ccagctcttt
     4261 gaccatgttg cggaagtttg gcgtgtggcc gtcttcgcga aacttgtcac gcatatagcg
     4321 taccacttgc cagtggccgt cggtcaggct gatgttttct gccgcggcga taacgggaac
     4381 gatgtcgtca tcgaagaccg gttcgagcag gaatttttcg tcatcgacct caagttcctg
     4441 tccgttgtag ctgtagctca tgttttcctc ctctgtgatg tcgaacaaac aatactctgt
     4501 gtgcgtgcca ggatcagacc gactccaccg agccgacggc aacgatggat ttgtgcggaa
     4561 tgaacagccc gcagcccagc ttgcggtgca ggccgatgcc gtgttgttgc actgcaatcg
     4621 cctgtgccag tggcaggttg tgcagcagca ggctgtagcc acgcacctgc tgcgtgccgt
     4681 catacagcag ttgcggcctg ccgcagatga agcgacactc gatgtccatg tctcgcagca
     4741 ggcgcagtac ctcgcgggca aattttgctt cgtcctcgtc gccggtgacg acgcaatggg
     4801 catacagcgg cgaatacgtg ctcagcgcgc gcgatttgcc tgcgccgacg accaggctgt
     4861 gttcgccgag catcagggtt ttcccc
//
LOCUS       DAIANL010000037         6502 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_646167, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000037 DAIANL010000000
VERSION     DAIANL010000037.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6502)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646167"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1399 a         2213 c         1826 g         1064 t
ORIGIN      
        1 cggctgccgc gtcagatcca gaacggcgcg ccagtaacga gaaaatcctg atggtgcacg
       61 acgacatcgc gcccgtcagc cagaatcaca ccaaatccgg gcgcctcatg gctgcccggc
      121 acatcagtgc cgcgagtgtg caggtccagc gccacctggt gattgggcga acgcacgcaa
      181 gaaaacggcc agccgcgcca acttccgccg atggcgcggt gcacgtgtcc gaccagcaga
      241 tgacgaatgc gcggcctgta cggttccagc acgcaagcca gcggttccgc attgcgcagc
      301 gcgtactgat ccatggaagg aatacccacc tgcagcggtg gatgatgcat caccagccac
      361 aacggttcgt cggcatccag tgtcgccagc gtgcgctgca gccattccag ccggtcagcg
      421 ccgtactcac catgggccac accgtactgc aaggtgtcca gcagcaggaa actgcccagc
      481 ggggttgaca cccgctgctg caggtacccg tgcgcatcga ccggggtctg cgtaaacact
      541 gcccgcaggt tcgcccggtc gtcatggttg cccggcagca gataacaggg catgggcaag
      601 cgttgcagct gctgttgcag ccaggcatag gccgcaacgc caccccggtc ggcaagatcg
      661 ccggtcagca ggcagaacgc cgcatcggca tgctcgcgta cgataacctg tatggcctgc
      721 tgcaaccgtg tggccggatt ggcgccaaac atgtccgcgc cgtcatccgt caggtgcaga
      781 tcgctcagat gcagcacctt catgcgctca gtcatggtgc tcaatcgggt tcccgtacag
      841 tggctcggcg ccaaagtggg tactcaccgc gtacgctgcg ccacaggcga gcatgaccgg
      901 cagtaccagg tgaaactggc tggtcatttc cagcaccatg acgatggcca tcagcggcgc
      961 atgcgtgaca gccgcgagca cagccgccat acagatgatg gccatcacca ccgggtcgcc
     1021 ggtcagcgaa ccgaacagat aacccgtagt cgctccgaca aacagcgtcg gcgtgaaaat
     1081 gccgccgatc gcaccagatg ccgtactggc caccgttgcc gcaaccttcg ccaccagcac
     1141 gattgcgacc cagtcccagg gcaagccacc attgagcact ttcgacacca cggtgtaacc
     1201 attgccccag acttccggga cgggtgcaga caacagtccg accaccaccc cccctagacc
     1261 cagacgcagc cagagcggac cgatcttcgc aaacagccgt ttcatccggg caatgagccg
     1321 cagcagcccc aaccccagca caccggagac aatgcccccg gccagagcgg caagcagcct
     1381 cgcgggagtg accgacaggg acggaacgac cggcatgaca tacagcgggc tgggttcgac
     1441 catccagaaa atgagcgcac tggccgtgac cgccgagatc agtaccggcg ccacagtgtg
     1501 gcgggcaaag aaacccaggg cgagttccag cacgaacacg acgccggcaa caggtgcgtg
     1561 gtaagcagaa gcaatgcccg ccgcgatgcc acacaccaga atcgcattgc gctgaccctc
     1621 cggaatggga aatatgcgtg ccaaccggga ggcaaaccat gccgacagct gcaccatcgg
     1681 tccttcacga ccgatcgccg cacccgagcc gacagacagc atcgaagcca gtgtgcgcac
     1741 cgcattggta cgatcgttga gcccggtttt tccttcgcgc gccgcatcga tgtagtcgat
     1801 atgcgcgtcg gtggtccagc gcgcgccgac ctgcaacacc agccccgcca gaatgccgcc
     1861 caccaccggg acgattaccc ggaccccggg cgcgagcgac aacgcattat gcaccaggct
     1921 gccggtgtgc cgtgtcaaca gccactcgac cgcactggtc aacaggtgga acgcatacat
     1981 tgccagcgca cccaatatgc cggcgagaaa tgcagcgagc cagagcaggg cggtgtcttt
     2041 tacgttgggt ctggcagcgt gctgtgtcat ctttattctt gttcatggca gggaatacgg
     2101 tggccggcct ggccacaggg acggtaccgt cagtcggcgc ggcgcgtccg cgcactcatc
     2161 cggccagttc cggtcgcccg aaataatacc cctgtgccca atcgatttcc agatcgcgca
     2221 gtcgattggc agtcgcttcg tcctcaacgt actcggcaat cgtgatcaaa tccagatcat
     2281 gtgcgatgtt gctgatgcgg cgcagaatct tctgcacgcg cttgtcgtga atctgctgaa
     2341 ccagggtccc ctcgatcttg agatacgaca ccggcaattc tgccagatag cggaacgagg
     2401 aatagccact gccgaagtcg tcgatcgcca gccgcatgcc gaaatcaaga aaaggcgcca
     2461 gcttggactt gaccgcttcc atgtccccca tgaactgccg ttcggtgatt tcgatcacca
     2521 tcggtttttg cggaccaccg gcgataccgc attgttcgca tgcctgcctg gcttcgtcga
     2581 acaacgcctg gataatctcc tgatgcaaca ggatatcggc cgacagattg acgaaatgca
     2641 gccggttggt gatgccgcca tgggtattgg cgacgcagtg ccccatggtg cgcttgatga
     2701 tctggaagtc gatcaggtgt gtgagctgca gctcgcttgc agcctggatg aaatcagcgg
     2761 caggcaacac attgccctgt tcatcgatca ggcgtgccag cgcctcttca gccacctgca
     2821 ccccggtttt cagatcgacg atcggctgat aggccggcac cagccggcct gccgcgatgg
     2881 ccgaatcgat cttcctgatg atggaatgga tttcgtgctg ttccatcgcc accacaatgc
     2941 ggttacgacc ggttttcttg gcttcgtaca atgcggcatc cgctgccctg agcaggatct
     3001 ccggcgagtc accatcccgc ggacagctcg cgacgccgat gctgaccgtg gtctggatct
     3061 cccgcccgtt gaaatcaatg cgcagctgct cgacgccact gcgcagctgc tcggcgatct
     3121 gcatcgcctc gcgttcgccg gtatccggca gaatcgccag aaattcttcg ccaccccagc
     3181 gcccgaccca atcgctgcgc ctgagcaggc ggccaaacag gtcactcaca ccgcgcagca
     3241 tcttgtcacc ggtttcatga ccatagttgt cgttgatcag cttgaaacgg tcgagatcga
     3301 gcagcaacag gctgaaatga cgggccgcgc gtttcgactg cgcatgcatg cgcgtgaaaa
     3361 actcctgcat ggcagcccgg ttgagcagac gcgtggtcgg atcgatggtc gacatctctt
     3421 cgagcttcat gcgcatgcgc gcctgatcca gaccaacact cgccagctgg gtaaaagcaa
     3481 agaacggctc gatgccgacc tgctcaaaat aacgcgccgt gcgggaaaaa atcacaatca
     3541 gcccgcgctg ttccatctcg tggtcgccaa tgggcagcgt caatccttcc ttcagaccaa
     3601 aaaaagcggc ctgttcgcgc cagatatgga aactggcatc gctttccaca tcgacgacca
     3661 cgggcatgtt ggctgccagc gagcgtcgcg ccggcccctt catcgcttcg ggcgagcgat
     3721 cgatgctgag cgattgcacg tattccgccg ccacaccggc tgcataggag gggcggatca
     3781 cttcccgttc cggattgccg agatacatcc atgccagaca gatatggctc gatagcgaag
     3841 cgagggcatt gcatgccgcc cgcagcacat cgtctggcgt attgcccaag gccaggctcg
     3901 atgtcgccga caacaatgcc tgatatatca gcaacttttc ctggatcgga tccatgagaa
     3961 ccctgaagtt tgcattcgcc atggtgcgca tgtttgcaca ttaaaacgcc aatcacaacc
     4021 gcagcatcat tgcccaggcg ctttttcctg ctgaccgacg cttgcggcag gcagattttt
     4081 tacgcggatc tttgtagaat gacgcctttg actctcatta tcattcaaca tgactgtccc
     4141 ggatgccctt gagaaacagt acaagtccat cccgctcgaa caccggctgg acatggcgct
     4201 gagcgacgcc ctgccagccg aataccgtcc cttcatggtg cgggaacaat ggatggtggt
     4261 gaagtgctac tttgctcgcc gcgccgacct gacggccgac gaagtcgcgg cactgctggc
     4321 agatcaggac catgtgatcc ggctgtgcat cgccaagcga cccgacctgc cggctgatct
     4381 ggtcgaagcc tgcgtgcacg accgcgaccc gaacgtgcgc cacgccattt cacgcaatcc
     4441 ctgcctgacg gacacccagc gccagcacct gctgacagat gtcgatccgc tggtggcgcg
     4501 cgccgccgcc aagggtgcca aacaggttca gtaccgccaa cgcccaggac aggccgtgct
     4561 gatccgttga ggcacctcac tgcagtgtct gatcacgcag cgccaccaga ggcggcacac
     4621 ggctgatgcc ccacagcccc gccatgcccg ccagcgtcac gctcaacccg cctgccgcgg
     4681 cggcaatcgc ccacaacgca ggatcaaagc gccagaccag ctgaaacagc gcactcagcc
     4741 gccaggcgag catgctggcc ccggcagcgg ccagcacccc cgataacaca cctatggtcg
     4801 caaattcggc cacctgagcc gcccacagct gttcgcgccg ggcgccgaac acccgccaca
     4861 acgcggtttc ctgcacccgc tccctgcggc tcgccatcaa tcctgcatac aacaccgcca
     4921 gtccggccgc cacgctgaac acaaacacaa actgcactcc catgctgacg cgatacaaca
     4981 tctgctgcag ttcctgcaaa atggcagaca catcgatgac gaccagattc ggctcggcac
     5041 gcaccagccc atccagacga tcatcctgtc ccggcggcat gcggaagctg gtaatgaaac
     5101 tcgtcggctg gccgcgcagc aagccttccg ttccgatcac aaagaaattg acatggaagc
     5161 tgtcccactt cacggcacgc agactggtaa tacgcgcact gaccggcgta ccggccacat
     5221 cgaatccaag acggtcgccc agccgcaaat gcaaccgctc cgcgattccc tgctccaccg
     5281 aaaactgggg tggcgcgcct gaacccgacc agcccgatcc ggccgacacg ccatcctgcg
     5341 ctccgaggct gacggcgtac gacagattga actcgcggtc gagcaacctg cgcgtctgcg
     5401 catccgcata tgcgccactg cgcacttcgt ggccattgat cgtcaccaac cggccacgaa
     5461 tcatcgggta caactgtacc gcaggcaggc cgttgcgacg aaaccaggcg agaaaatcgg
     5521 tacgttgatc cggctgcaca tgaatgatga aacggttggg tgcattggcc ggcaaagtgg
     5581 cttgccagcg ttgcagcaat tcgtcacgca ccacggtgag caacagcaag gccagtatcc
     5641 ccagcgtaaa cgccacgatc tggatcacgc tgcccaacgt gcgccggcgc aggttgagca
     5701 gcccctggcg ccagctgcca cggccgcgct ccccgatcca gccgatcatc tgcaccagca
     5761 accaggcgac tgcagcggtc gatcccagca ccccgccgac cccgacaagc acccgggccg
     5821 tcaatccggc atcgccagtt tgccagaaca gcagcgccgt caaagtcacc agtccggcca
     5881 gtgccgccag cacaccatcg taccggctac cgcgcccgct ggcttcgcga cgcaaaatac
     5941 gtagcgccgg cacacggcgc aacgccagca gcggtggcag gctgaacccg gccaacagca
     6001 ccaaccccgc cagggcagcc tgaacgaagg gtaaggccga cgccgccggc agttcgagct
     6061 gcgccagccg ccccagcatc tgcgccagca ctgcctgccc accccaaccc accagccccc
     6121 caatcagcgc cgcaagcact ccgagcaccg taagttgcgt ggcgtatagc gtgatgatgc
     6181 ggtgctggct ggcaccgaaa cagcgcaaca gggcgcaggc atcaagatga ctgcgcacga
     6241 actggcgcgt cgcaagcaac accgcgacgg acgacagcag caccgaaagc agtgcggcca
     6301 accgcaggta acgttcggcc tgatcgagca cttcgcgcac ctgcggccgg gcatcctgca
     6361 gactcaacag ccgctggcca cgctccaggc ggggcaacaa cacctcacgt tcgtgctgca
     6421 catcccggct ctcgccggca aacagcgcat ggtaatccac gcggctgcct tcgacgacca
     6481 gtccggtggc cggaagatcg gc
//
LOCUS       DAIANL010000038         4994 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_37355, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000038 DAIANL010000000
VERSION     DAIANL010000038.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37355"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          937 a         1620 c         1445 g          992 t
ORIGIN      
        1 catggaacag attgccggat tcgcgctgcc ggtacatgtc aatccggcac tggtgcgcag
       61 caatgaggtg cgccggctca tcggctcacc tgcccgtctg gaacactgca tcggccccca
      121 gacccgtatc ccgtttgcgg atacactgcg ctggatgtac gacagcgcac gcgccgcacc
      181 gcccgccggc tgaaccgcag ttcccgctgt acgaccgcgg cgaacaggct cagcccgtct
      241 ggtagcgcgt cgggtcggga atgcctgctt cctcgaaacc acggtgccgc agacgacatg
      301 catcgcaacg cccgcaagcg gcaccgctct catcggcctg atagcaggac acggtcagcg
      361 aaaaatccac acccagacgg gtgcccagac ggatgatctg tgccttggac agacgttgca
      421 gcggcgtatg gatagtcatg atgcggccct cgacaccggc acgcgtcgcc aggttggcca
      481 tgtgggcaaa cgcctcgatg tattccggcc ggcagtcggg atacccggaa taatcgaccg
      541 cattgacccc gataaagata tttgcacaat cgagcacttc agcccaggca agcgcaatgg
      601 acagcatcac ggtattgcgc gctggcacgt aggtgagcgg aatgccttcg ctcacggcct
      661 gtggcaccgc aatctgcgga tcagtcagcg cagaaccgcc aaacatggcg agatcgagat
      721 gaaacacccg ctgttcgacc gcgcccagca gcttcgccac acgcgatgcg gcatccagtt
      781 ccgcgcgatg acgctgacca tagtcgaaac tgagcgcatg gcaggaaaaa ccgcgctgca
      841 ccgcctgcgc cagtaccgtc gcggaatcca gcccgcccga caacaacacg atcgccgctt
      901 cgctcatcag cgtccccttg catcgtccca caacagtttg tgcaattgca gctgcaggcg
      961 caccggcagt cgatcgcgca ggatccactc cgccagttgc cgggcattgc tgccatgtac
     1021 cggcgaaaac agcactggac aacgcgctgc cagatcgcgg ctgcgcagca tctcccgcgc
     1081 ccactcgtaa tctgcctcgt ctgtcagcac gaacttcagt tcgtcgcgtg cgttgagcac
     1141 gtccagattc tgccagagat tgcgttccat ttcaccagaa ccgggcgtct tgatatccac
     1201 gatgcgtgaa acccgcgcat cgaccgggcg aatgtcaagc gcgccactgg tctccaggga
     1261 tacatcgtaa cctgcgtcac acagggctgt cagcagagca ggtgtgccgc gctgcgccag
     1321 cggttcgccg ccggtcacac acaccagcgc agacccatgc aatgccaccc gagccagcac
     1381 ttcgtcttgc gacagtgact cgccaccttg aaacgcgtag gccgtatcac aataaccaca
     1441 acgcaacgga caaccggtca ggcgaacgaa cacggtgggt cgccccgccc ggctggtttc
     1501 gccttgcagg gaatgaaaga tttccgtgac gcgcagcgtc cctgccataa aacggtcagg
     1561 caccctgcaa catctgcagg cgtttcttgc cggtttccgc cgccggcgtg cctgggtatt
     1621 ttccgatcag cttctgtaat gtcttgcgcg cggcggccat gtcacccagt tcaatctgcg
     1681 cactggacaa gttgagcatg gcgtccggca ccttgggact tctgggatag ctcgcaatca
     1741 gcttctgctg cgctgcctgc gcgcttttaa aatcccgggt ggagaaatag gcattgccga
     1801 tccagtattg tgcactggcg gcaagcgcat catcaggcca ggtctggatg aacgatctga
     1861 agctgctgat cgctccggcg tagtcgccct gtttgaaccg ggtcagggca gcctgatagg
     1921 cttgtgcctg ctgtgtcgcc gccgaagcgg gcgggttgtt gccggctccc gcggaaccgg
     1981 actgggatgc cgcggcagtg tcgtcaaccg ccgtaccggc ggccgcgccc cctgccggag
     2041 cagatgaatg ctgcgatgct gccgctgcgc cattctggct caacgcacgc aatcgggtgt
     2101 caaggtcctg gtacagatcg gtctggcgct tctccgccac ggacagttca tgcgtctgca
     2161 cgtcaagcat gccgtgcaac tgcgcaatct cgtccttgag acgatcgatc tcggactgca
     2221 attttagcaa ggctcccgca ccggactggg catcgagctt gtcctggcgc tgggagagcg
     2281 cgtcaagctg ctgttgcatg gttgctaacc gcgctgcggt atcatcatcc atggcttgcg
     2341 ccggcaatgc cagcgcaagc catgcaagca acgcgaatga cgcgcgtcgt gtcataccgg
     2401 attactcgct ctgataaacg atgtcggtac ggcgattctg cgcccaggcg gtttcgttct
     2461 cgccctgagc gcgcggcttg gacttgccga agctgatgga ggacatctgg gcttccggag
     2521 cacccaacag gctcagcgac ttgcgtacag agtcagcacg acgctggccc agcgccaggt
     2581 tgtattcaac gctgccacgg tcgtcgcagt tgccttgcag ttgtaccttg gcgttgctgt
     2641 gatcttcaat gtacttggca tgcgcttgca gcatgggctg atattcgctg ccaacgttat
     2701 acttgtcgaa ggcgaaatac acgctgcgtt gcgacaggat gttgttcggg tcgtcgagcg
     2761 ggttgatctt ctgcgcagcc agagcagctt cacgagcgcg ggcttcggcg gcagccttgg
     2821 cggcggcatc ggcagcagcc ttgtcagcag cggccttggc ggctgcagcg cgggcagcat
     2881 ctgcctgcga ggtatccggc ttggtctgtt cgctcgaaca gccagccagc agacccacga
     2941 taaccaatcc cagtactgtt ttcttcattt ttcagctcct tgatttaata aaatacaacc
     3001 ctgaccagca tacctcatac gacacggctg ccgcaaccga cgacgagccg tgatacacca
     3061 acagcgctgc caacctgcta tggtccccag atcggctgtt ccatgagccc ggcggcattg
     3121 gacagccgct catgggtttc gccatccgaa gacaccacgg ccagttcgcc gcgaccattg
     3181 acctccgccg cgtagagtat cattttacca ttcggcgcgt aacttggaga ctcgtcgtcg
     3241 atgccgctgc tcagcacctg cgcctgcccg ctggccatat ccatcactgc gatgtggaat
     3301 tggccgtgca tgcgctgcac gaatacgaaa ctcttgccat ccggactcca gcgcgggctg
     3361 acgttatagc tgccgtcgaa cgtcaggcgc gtcgcattgc caccgtccga tggcatcatg
     3421 tacacctgcg gcgtaccatc gcgtgccgag gtaaacagca gcgtcttgct gtccggactc
     3481 cagcacggtt ccgtatcgat accgccgcca aaagtcagcc gcttcagatg cgagccatcg
     3541 gcattcatca catacagttg cgaaccgtcg tcccgtgtca gcaccagcgc gatccgcttg
     3601 ccgtccgggc tccaggccgg cgcgctgttg ctgccgggaa acgcagccag cacgaaccgc
     3661 ttgccggtat caagcgcctg ctcgtagaca atcgcccgac cggtctcgaa agaaacgtag
     3721 gccatgtatt tgccatcggg actccacgcc ggcgacatga gcgcttcctt cgacttcagc
     3781 acggtctgcg ggttctggcc gtccgaatcg gcaatctcga tccggtattg gctgccttgc
     3841 tggatgatgt aggcgatatg ggtcgaaaac acgccgggca caccagtcag tttctcgtat
     3901 acgatatcgg cgatatggtg cccgaccatg cgcaactgat ccggtctgac ggtatagctg
     3961 taaccggcga gctgggtttg ctgcacggcg tccatgagac gaaaacgcac ttccagcctg
     4021 ccatccggct gcggcagcac atggccgagc acgatcgcgt ccgcaccacg agcccgcgcc
     4081 atgccaaaac gcggatcgtt gggttccgca atcggcggcg caatgtcgct gacatcgacc
     4141 atgctgatct cgccggtacg cagcagatcg gccgccagca catcggtgac cgtgcgatag
     4201 tcctgtcctg cgccagcaaa tggcgaaatc gccactggaa tgcgtgttgc cgcaccacca
     4261 gtaatgtcga tggtcatctg tgcccgtgcc ggcaccagcc acagcaccca gagacatgcc
     4321 agccagacgg ccagccatcg tcgcatattc attgtgttgt tactccgatc atttcgtttc
     4381 atccggtgaa aagtgcaggt gcaggtcacg aaacatgccg gccaactgag ggtcgggcgg
     4441 caacggcaac ggtgacgcct tgtaaatggc ccgctgtact gcatcgtcat acgccggttg
     4501 cccgctggca tgcatcaggg tcaccctgag cacttcgcct gtcggcagca gcgcaacgtc
     4561 aaacaccgct tccgggttcc cctgcaaccc gtcaggcaga atgatccggc tgcgaatctt
     4621 ggcgcggatg cgctgctgat attcatcgag caggcgctgt tgctgccggc tggcctgttg
     4681 cccggcgacc accatcgcct gcgcacctgc cagcgcatgc tcggtttcac tgcgaatgcc
     4741 ggcctgcgtc tgctgcacca gcatctgcct cagcgccagc acgcgttgct gctgttgctg
     4801 ctgcgccagt ttcagtgcgg cctgcttctg ctcctgctgt tgctgctgct tctgtatttc
     4861 ctgctgctgg cgctgctgct cgagtttctt cgcctgctgc tgtttcttct tttccgccag
     4921 tgcgatgtct gccgcaggct cgccgtcagg cggcgatggc gcctcgggcg tcggcacggg
     4981 ccgcggttcg ggcg
//
LOCUS       DAIANL010000039         6637 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1059524, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000039 DAIANL010000000
VERSION     DAIANL010000039.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059524"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1263 a         1855 c         2142 g         1377 t
ORIGIN      
        1 gaaggcatgc atgtgcccga gggcgaagcg tattcggcgg tggagcatcc caaaggcgag
       61 ttcggcatct acctggtgtc ggacggcgcc aacaaaccct tccgcatgaa aatccgcgct
      121 cccggttttg cccatctggc atcgctggat gaaatggcgc gcggacacat gattgccgac
      181 gtggtggcga ttattggtac gcaggatatt gtgttcggag aaatcgaccg gtaatcggtc
      241 gcggtaatcc gtactgcagg tggaaagcag aaagaatagg ggtgtctgag atgttgagtc
      301 cggcgtcact ggagcagatc gatcgagaag ttgccaaata tccggccgag caccggcaat
      361 cggctgtcat gtccgcattg cgcattgcgc aggtggagaa agggtggctg tcgaccgacg
      421 tgatggattt catcgcgcat tatctggaca tgccgagcat tgccgtctac gaggtcgcca
      481 cgttctacaa catgtacgat acgcatccgg ttggcacgca caagctgacg ttgtgcacca
      541 atatttcgtg catgttgcgc ggtgcgccgg agatggcgga ttatctcagg cgaaaactgg
      601 cgtgcgaatt cggtgagacc actgcggatg gcctgtttac cctcaaggaa ggcgagtgca
      661 tgggcgcatg cggcgatgcg ccgctgtgtc tggtcaacaa ccatcgtatg gaaggttttc
      721 tgaccgaaga aaaaattgat gccctgatcg aggcgctcaa atcggaaaag aagggtgcgt
      781 gaatggtaat cgttggcgga gaggtcattt tcaacacagc ccatcttcct gaatcgtgga
      841 agctggagac ctatctcgcg catggcggct acgctgccct gcgcaaggtg ctgacggaga
      901 aaattccggc ggagaccatc atcgccgagg tgaagaaatc cgcactgcgc ggacgaggtg
      961 gtgccggctt tcccacgggc ctcaagtgga gtttcatgcc gcgacattat cagggagaga
     1021 aatacctggt gtgcaacagc gacgaaggtg aaccgggcac cttcaaggac cgcgacattc
     1081 tcatgtacaa cccgcacgcg ctgatcgaag gcatggccat tgctgcctgg acgatgggcg
     1141 tgcaccgggg ctataactac atccacggcg aaatctggca tgcgtacgag cgcatggaag
     1201 ccgcgctcga agaggcgcgc gcagcgggtt ttctcggtca ggacatcctc ggcagcgggt
     1261 ttgatttcga cctgttcaac caccatggtt atggtgccta catttgcggc gaagagactg
     1321 cgctgctcga atcgatcgag ggcaagaaag gccagccgcg cttcaaaccg ccgttcccgg
     1381 ccagcttcgg cctgtatggc aagcccacaa ccatcaataa caccgagact ttctccagca
     1441 ttccgtacat cattcgcgag ggcgggcaga agtttcttga gctgggcaag cccaacaacg
     1501 gcggcaccaa gatcttttcc gtgtcggggc atgtggaacg gccggggaat tatgaagtgc
     1561 ccatgggaac gccgttttcg aagctgctgg agatggccgg cggtatccgt ggcggacgca
     1621 cgttcaaggc gtgcattccc ggagggtcat ccaccccggt cgtgccggcg gagatcatga
     1681 tggactgtac catggactac gattcgatcg ccaaggccgg ttccgcactc ggcgccggct
     1741 cggtgatcat catggacgac agtgtatgca tggtgcgtgc actggaacgc ctgtcgtact
     1801 tctactacga ggaatcctgc ggccagtgca cgccctgccg cgagggaacc ggatggctgt
     1861 atcgcgtcat tcaccgcatc gagcacggtc agggtcgtac cgaggatctg gcgctgctcg
     1921 acgacgtggc gcacaacatc agtggccgca cgatctgcgc gctgggtgat gctgctgcgg
     1981 cgccggtgca agccatgctc aaacattacc gccgcgagtt cgagcatcac atcgaacacg
     2041 gccaatgcat ggtataggtg gacttatgct ggaaatcgaa atcgatggca aaaaggtagc
     2101 catgccgacg ggcggcacca tcatggatgc cgctgctctg gcggggattt acatcccgca
     2161 tttctgctat cacaagaaac tttccattgc cgccaactgc cgcatgtgcc tcgtgcaggt
     2221 ggaaaaggcc aacaagccgc tgccggcgtg tgccaccccc gtcacggacg ggatgaaggt
     2281 gtccacgcgc tcggaggttg ccgtgcgtgc gcaaaaaggg gtgatggagt ttctgctcat
     2341 caatcatccg ctggattgcc cgatctgcga tcagggcggc gagtgcgcac tgcaggatct
     2401 ggctgtgggc tatggcgggg tcggatcgcg ttatcaggaa gccaagcgcg tggtgacgga
     2461 aaaagacctg ggcccgctgg tggcgaccga catgacgcgc tgcatccatt gcactcgctg
     2521 cgtccgcttc ggacaggaaa tcgccggcat catggaaatg ggccagaccg ggcgcggcga
     2581 gcattccgaa atcatgccgt tcatcaacca gacaatcgat tccgaactgt cgggaaatat
     2641 catcgatctg tgcccggtcg gcgcgttgac cagcaagccc tttcgttata gcgcgcgcac
     2701 ctgggaattg tcccgtcgtc gttccgttgc gccacatgac agtctgggca gcaatctggc
     2761 gatacacgta aaagaccagc gcgtgatgcg tgtgctgccg ctcgaaaacg aagccatcaa
     2821 cgaatgctgg atctccgacc gtgatcgctt ctcctatgag gggctgaatt cgccggaccg
     2881 tctgacccgg ccaatgctca agcgcgatgg ccagtggagc gaagtcgact ggcaggtggc
     2941 gctggagcac gttgccagtt cgcttcagga catccgcaca cgtttcggta ccgacagcat
     3001 cggcgcgctg gccagtccga cgcagacgct cgaggagttg tatctgttgc agaagctggt
     3061 gcgtggcctc gatagcggat atgtcgacgc ccggccacgt caggccgatt tcggcgccga
     3121 tgcgcatctg ggcggcgctc cgtggctcgg aatgccggtc caggacatca accagctgga
     3181 tcgcgtgctg ctcgtcggtg gccggttgcg tcgtgatcac ccgctcattg cgcatcggtt
     3241 gcgggtggcg gttcgccatg gcgcacattt cagcctgatc aatccggtgg atgacgactt
     3301 gctgatggcc gtgtcgcata aatccatcgt ggctccgcag gccatggagc agcagctggt
     3361 gcaactggcc gctgcactgc agcgcaagac cgggcgcaat gtgagcgacg cggtggttgc
     3421 gcggctggcc ggagtcgttc ccgacgccgc tgcgcaggcg atggcggacg acctgctcgg
     3481 cggcgaacgc cgcgcgatcc tgcttggtaa tctcgcgcag caacacccag gctatgccgg
     3541 gctgcacggc tgggcccagc aggtggccga cctgtgcggc gctacgctgg gtgtgctagg
     3601 cgaggcggcc aacagcaccg gtgcgcgtgt ggccggagcc tggcctgcag tcggcaagga
     3661 tgcgcgagcc atgatcgagg tgccgcgcaa ggcctatgtg ctgctcggac tggaaccgga
     3721 atttgattgc cacgacccgg tacgcacggc agcggccctg cgttcggccg ggctggtggt
     3781 cggactgagc gcatttcgtc cgaccgacat gagcgtattc gacgtgctgc tgccggtggc
     3841 gccgtttacc gaaaccgcag gcaccttcgt caatgccgaa gggcgcgtgc aaacgttcaa
     3901 aggcgcggca caaccgctcg gtgaaagccg tccggcgtgg aaagtgctgc gtgtcctggc
     3961 caccatgctt ggcatggaag actgcgccta cgacagtgtc gaagcggtgc gtgccgcgct
     4021 gctgccgacg gggcaactgc ccaccgcggc gctgaacaac cggctcgaag gctggcagcc
     4081 acagtcccat ggcgaaatgg ctgagacggt ctggcgactg ggtgatgtgc ctgcctaccg
     4141 ttgcgatgcg ctggtgcggc gtgcagcgtc tttgcagcgc gtgcgcgaca cggaagccga
     4201 cgcagcgagc gctcatccgc agtggatggc tcgggcagga tgggtgtcgg ggcagcgggt
     4261 gcgtctgcgc cagggagatg caacgacgga aacagtgctg ctggccgacg agcgcgtggc
     4321 gcctggttgt gtgcggcttg cggctgcaca accactggcg gtggcgcttg gggccatgat
     4381 gggcgaaatc agtgtggagc gggcatagca tggcgtatct gcaggcgtta ttcgggatga
     4441 gctggccgct ggtgtggtcg cttgcaaaaa tcgttgccat cgtggcgccg ctgatggtcg
     4501 gtgtggccta tctcacctat gccgaacgca agatcatcgg cttcatgcag gtgcgtatcg
     4561 gccctaaccg cgtgggctac aaggggttgc tgcagccgat cgccgatgcg gtcaaactgc
     4621 tgatgaagga aatcgtggtg ccgaccgggg cgaacaagtt cctgttcgtg ctgggtccgg
     4681 tgctgaccat cgcccccgcg ctggcggcct gggcggtgat accgttttcg cccgatctcg
     4741 tgctggcgaa tgtcgatgcc gggttgctct atatcatggc gatcacttcg atgggggtgt
     4801 acggggtggt gatcgcaggc tgggcatcca attccaagta tgcgtttctt ggcagcctgc
     4861 gttcggcagc gcagatcgtg tcgtacgaga ttgccatggg ttttgcgctg gtcggcgtgc
     4921 tgatggcagc acacagtctc aacctgacgg acatcgtcaa cggacagcat ggcggcttcc
     4981 tgcactggta ctggctgcca ttgttcccgt tgttcatggt ctacctgatc gcgggtgtgg
     5041 cagaaaccaa ccgcgctcca ttcgacgtgg ccgaaggcga gtctgaaatc gttgccggct
     5101 tccacgtgga atattccggc atggcgtttg ccgtgttctt tctcgccgaa tatgcgaaca
     5161 tgattctgat cgcgacactg acgtcgatca tgtttcttgg tggatggaac ccggtattcg
     5221 attttgcacc gttcaactgg ctgccaccca tcgtatggct gctgggaaaa acaggttttg
     5281 tgctgttcct gttcctctgg ttccgggcca cgttcccgcg ttaccgctac gaccagatca
     5341 tgcgcctggg ctggaaggtg ttcatcccga tcacactggt ctggatcctg gtggtgggcg
     5401 cggtcatgca gacgccgctg gcgtttctgg tgcactgatg actgcggcga cggagtaagt
     5461 tatgaaagcg atcaaacagt ttttttccag cctgctgctc atcgagctgt tgcgcggcat
     5521 ggctctgacc ggacgtcacc tgttcgcgcg caagattacc gtacagtatc cggaggaaaa
     5581 gacgccgcaa tcggcacgct tccgcggcct gcatgcgttg cgtcgttacg agaacggtga
     5641 agaacgctgt atcgcctgca agctgtgcga ggcggtgtgt cctgccctgg cgatcaccat
     5701 cgaatcagag cagcgtgcag acggcagccg ccgcaccacg cgctatgaca tcgatctgac
     5761 caagtgcatt ttctgtggct attgtgagga atcctgcccg gtggattcga tcgtggaaac
     5821 gcactttttc gaatatcatg gcgagcagcg tggcgacctg tattacacca aggaaatgct
     5881 gctggcggtc ggtgaccgtt acgaggccga gattgcggca gcgcgggcag ccgacgcgaa
     5941 atatcgttaa gcggagcgga tacgtacaac atggatttcc tgacaggcgt tttctacttt
     6001 ttctcggcgg tattgctgtt tagcggcttc cgtgtgatca ccacccgcaa tccggtgcag
     6061 gccgcgctgt tcctcgtact ggcttttttc agcgcttcgg cgatctggct gacgctggag
     6121 gcagagtttc ttgccattgc gctcgtgctc gtctatgtcg gcgcggtcat ggtgttgttc
     6181 ctgttcgtgg tgatgatgct cgatatcaat ctggacagat tgcgcgaagg cttctggagt
     6241 tatctgccac tggcgctgtt cgttgccacc atgctggtac tggaaatggc aatggtgctc
     6301 ggcgcacgca ccttcgggct caacatcgtt ccggcgccgg tgcctcatgg cgcgggttac
     6361 agcaatacgc gcgaactggg gcgattgctg tatacggatt acctgtatcc gtttgaactt
     6421 gcagccgtgg tgctgctggt ggccatcatc gcggccattg cgttgaccat gcgtcaccgc
     6481 aaaaatacgc gctatcagaa cccgtcgcaa caggtgcggg tgaagcgcga ccagcgcgtg
     6541 cgcctggttt cgatgccgtc tggcgatacg ccgacgaacc agccgtgaat acgagatacc
     6601 agaaagggag aatgccgtgc tgtccttgtc gcattat
//
LOCUS       DAIANL010000040        12702 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1061483, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000040 DAIANL010000000
VERSION     DAIANL010000040.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 12702)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12702)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12702
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061483"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2411 a         4067 c         3818 g         2406 t
ORIGIN      
        1 taacggattg atttccttta tattgcttgg ctctgtcgcg tactcgccgc gaagatctga
       61 gataaaggac cccccatgaa agctgacatc catcccgaat acaccgaaat cgacgtgacc
      121 tgctcctgcg gcagcacgtt caagaccggc tccaccctgg gcaagcccct gcacgtggaa
      181 gtgtgctccg agtgccatcc tttctacacc ggcaagcaga agatcgtcga taccgctggc
      241 cgcgtcgaga aattccgtca aaagtacggt atgaaataat ttccgcagta cggcagtcag
      301 tcgcccctga ctgccgttcg ctgacggatt tgcggtagaa tcacagccat tccatcgtag
      361 gctgccgttt gccgtgccca ttcccgacca agaaccgcgc ctgttacctt ttctgctgat
      421 tgtcgtgctg tgcctggcgt ggctggcccc gggcctgatc ggtcaccaac cgtggaaggg
      481 cgacgaagct gaaaccatgg ggctggtgca cagcatcctc tcgggtgccc ctgctgccac
      541 acccgttctt gcgggtgaac cctggatcgc cgccccccct cctttttacg cactggtcgc
      601 agccaccatc gcgcaatggc tggccccctg gttgccggca cacgacggtg cacgcctggc
      661 aggcgggttc ttcatcctgc tggcgctgat cttctcggcg ctgaccggca atgaactgta
      721 tggtcaccgc cacggccgtc tggtcgtgct ggtaatgatc ggcaccgtcg gactgtggtt
      781 cacggcacac gaatccattc cagaatctgc cctgctgtgc gccgaagcca tgaccggctg
      841 gggtgccgcg cttgttccgc gccgaagcct gacaggcggc atgcttgccg gaacaggcct
      901 tggcatggcc ttcatgagcg ccggactggt cgtggccact gcgctggccc tgcttctgct
      961 gctgctgccg ttcgtcagcc cggtgtggcg cacgcgggct acgctgcgcg ccgccgtcat
     1021 gtcagtgctg tttgcgctgc cctggtttgt gctctggccg tggatgctgg tgcattcggc
     1081 acccggctgg ctcgcagcgt ggtggcaaca tcagtaccag atgttcgtct tctgggcacc
     1141 cggccacgag acgcccacgc tgtattacct gcaatttctg ccctggtttg cctggccggc
     1201 gtggccactg gcaatctgga cggtatggca ggcacacaaa cgtgaccaga tgaccagccc
     1261 gggcatcgcc ctgcctctgg ccatgtggct cgtcatcact ctggtcatga gctgtacgcg
     1321 cagccccaac cccgatcacg gactggtact gctgccgccg ctggcctggc tggctgccgg
     1381 cggcacggcc acactgcgtc gcggtgcagc caatgcgctg tactggtttg ccatcatgac
     1441 cttcggcgta ctggcgatca ccgcctggat ttactggagc gcgctggatg tgggctttcc
     1501 tgcgcaactg gcgcaacacc tgcaccagtt gcagccggag taccagggta cccttcaccc
     1561 gatgtccgtg acgcttgccc tcgcgtactg cctgttctgg ctgatgctgc tggcacgcat
     1621 gaaacgctcg acccagcgcc cgctgatggc ctgggctgcc ggcatgacgc tggcctgggg
     1681 actggctgcc ttcctcttcg tctacccgat ggaccagcgt ctcggctacc agtcgatgat
     1741 gcgttcgctc ggccccatgc tgaccgacca gtcgtgcgtc tccggactcg atctcgatca
     1801 tggcgcacgc gccatgatcg actattatct ggatgtgcaa acgctgcacg tccgggcacc
     1861 ggacgaccgc agctgccggc aactgcttgc ggaggaaccc ggcgataccg ccgcgcctgc
     1921 ccgttatccg ggctggcagc tggtctggca aggcggccgg atcggtaacc atgtagaacg
     1981 ctttgcactc taccgccgcg ctccttcgac gcgtacgcat gcaatgggac acccatctca
     2041 tggccgggac tgattccagc accgaatacc agctcgacat ccagggcatg acctgcgcat
     2101 cctgcgtgac gcgggtggaa aaagcgctcg gccgcgtgcc gggggtaagc tcggccagcg
     2161 tcaatctggc caatgaacgc gccaccgtgt acggcagctc cgcagtcacc caggctcaac
     2221 ttgccgccgc cgtgcgcgcc gccggttatg aagtggctct gcacccgttc gatctcggca
     2281 tacgcggcat gacctgcgcc tcatgcgttg cgcgagtaga aaaagcgctt gcccgcgtgc
     2341 ccggagtcag cgaagtcgcc gtcaaccttg caaccgaacg cgcacgcatc ctcgccagca
     2401 gcgccgtatc catggccgat ctgcgccagg cggtgcagac agccggcttc gaacccgtcg
     2461 aggctgcccg gacagaagac gcaccgcaag ctacggcgcc cgactggctg ccggtagcac
     2521 ttgccgcgct gttcaccctg cctcttgatc tgcccatgct ggccatgccg ttcggtctgc
     2581 acatcgagct gccgggttac ctgcaatggc tgctcgccac accggtacaa ttctggctcg
     2641 gcgcccgttt ctaccgtgcg ggctggcatg cactgcgcgc cggctcgggc aacatggacc
     2701 tgctggtcgc cctgggcact tcggcggcct gggggctgtc ggcctggctg ctggcacagg
     2761 gcaacgccca cgcgttgtat tttgaggcat ccgcgaccgt catcaccctg gtacttctgg
     2821 gaaaatggct ggaatcgcgc gcccgcaaag ccacctcatc cgccatccgt gcgctgtcgg
     2881 cactgcggcc ggaccgggcg cgcgtgcgtc gcggcgacct gcaggaagac attgccctcg
     2941 acgaactgcg tgttgacgac atactcatcg tacgtgccgg tgaacgcatt gccgccgacg
     3001 gactggtggt ggccggcacc agcgccgtgg atgaatcaat ggtcaccggc gagagcatgc
     3061 cacaggacaa aaccacaggc gatcgattga tcgccggcac ggtgaatcag cagggcgtgc
     3121 tcgaggttca ggttaccggc acgggcaacc ggaccctgct ggcacagatc atccgccagg
     3181 tggaaaacgc gcaggccgcc aaggcgcccg tgcagcggct ggtggatcgg gtgagcgccg
     3241 ttttcgtgcc cgtcgtgctg ggaatcgcgc tactgaccct ggctggctgg ctgcttgccg
     3301 gagcgaattt cagccacgca ttgctcgatg cggtggcggt gctggtcatc gcctgtccgt
     3361 gcgcgctcgg cctggcgaca ccaacggcca tcatggccgg aacaggcatt gccgcgcggc
     3421 acggcatcct gatcagggat gccgaagcgc ttgaactggc ccaccgcgtg cgttgcgtag
     3481 ccttcgacaa gaccggcacg ttgaccgccg gccatccggt gctgatcgac tggcaaccgg
     3541 cgccgggttt cgatcgtgac ggcgtgctcg aacgtgccgc cagcctgcag cagaccagcg
     3601 aacacccgct ggcgcgcgcg gtgctactcg cagcccgcgc agccggtgtc gtgccacagc
     3661 cagtcacgca ggcgcaggcg ctgcccggtc acggcctgag cgcccaactc ggcgacgaga
     3721 ccttgtggat gggcaatgcc gcgctcatgc gctcgcttgg cctcgaagtc gccgaggtca
     3781 gcaatggcga ctcgcggaca cgcgcctggc ttgccagcca gcaggcgacg caggtctcgc
     3841 tgctcggcga tttcgccttt gacgatccgc tcaagcccag tgccgcaaca gccattggcg
     3901 aactgcatgc gcaggggata cataccgtca tgctgaccgg ggaccacgct ggcagcgccg
     3961 ctcgcgtcgc cggcgaactt catctcgacg aatatcgcgc caacatactg ccggcagaca
     4021 aggccgccgc ggtgaacgca ctgcgtgcac agtacggccc ggtggcgatg gtcggagacg
     4081 gcatcaacga tgcgccggcg cttgccgcat ccgatgtggg cctggccatg gccagcggca
     4141 ccgatgttgc catgcagacc gccggcatca cgctgatgcg tggcgacccg ctgctggttc
     4201 cggcagcatt ggacatctcg cgccgcacct ggaacaagat ccgtcagaac ctgttctggg
     4261 ccatgattta caatctggtc ggcattccgc tcgccgccgc cggcctgctc aatccaacca
     4321 ttgccggcgc agccatggcc ttcagcagcg tgagcgtggt gagcaacgcg ttgttgctca
     4381 gtcgctggaa gccggcaccc ttgcgtgcga gcacgaaatg accgatcgcc actggctgga
     4441 acaggcagat gcggaacagg tgctgcaagc ggccattcgc aacgacgacg acatcggcgt
     4501 gctgcaggcg cgcctgttac agataggtga cgcggagcag tgctaccgtt tcgcgcgcga
     4561 cgtgatggat gccgacattc ctccgctcca ggctcgcgtc atcgaaaacg gcagcaacga
     4621 actgtgcttc cgttttgcgc gcgacatcgt cggtgccacc aatgccactc tgcaacaacg
     4681 catcctgcgc acaggaagcg ccatggactg ttacctgttt gccgaggaca ttttcaatgc
     4741 cgacgtagag cggctgcgag cgcgcatcat cgaactcggc gacggcgagg tgctggatct
     4801 gttcgcagcc tcgttccccc cgcctgatat ggcctgaccc gccgccgtct cagcgcgtca
     4861 gcgaaagaaa cagccgcggc aactgctccg gcaaccgttc gacgtgatcg atcaccgcgt
     4921 gatgcttgcc gaagatgtcc tgcacgtatt cgtcggcgcg cggatcgagc gaaatgcagt
     4981 atgcctggat gccgtcgcgc gacagttcgc gcacagccat gcgtgcatct tcaatcagtg
     5041 tccgcggatc cttgctatcg atatcggccg gctcgccatc ggtgagcacg agcagaatgc
     5101 gcttgtcggc ctgctggtgc gcaaggtaat gccctgcatg acggattgca gcgcccatac
     5161 gtgtcgagta accggcttcc atggctgcca gtcgcgcctt gaccggagca tcccagcttt
     5221 cgccataccc tttgatatgc agatagcgta cttcgtgacg ggtattggaa tagaacccgc
     5281 cgatcgcata gcggtctccc atttctgcca tcgcccagcc cagcaggctg accgcttcct
     5341 ggctcaatgc aagcttgctc tgctggcaac cgggtggcac ctcgtcgagc gaagccgaca
     5401 gatcgagcag caccgataca gcgatattgc gcccgtcgtg ccgatggctc aaattgatgc
     5461 gcggatcggg agtggtgccg ctgcgataat cgatcagcgc ccggattgct acgtcaaggt
     5521 caagctcttc gccttcttcc tgatagcgaa tgcgcacctt gttctgcggc ttgagcagat
     5581 ccatgatctt tttcagctgc cgggccagtg gctgatgacg gttcaggata cgatcgatct
     5641 gtcctgcgtc gcctgccggg tgcagatgtt catatacgct gacccagtcg ggacgataat
     5701 acccggccgc gttgtcccat tcgggataca accgcggtgg cagacgctgc gcgtcgtcgt
     5761 tcgcggtgtc gggtggcctg gccttttcgt cctggacctc ctcgtcacct tcctcgatga
     5821 actgccacat caggcggtta tcatcgcggt aatccacctg cgtgtcctca aaccagacac
     5881 gggcgccaaa gtcctgcggc aaccgggtgc gggcaatgaa ctgtacgccg agggtcacca
     5941 tggcctcggt cgaactttcg cccgcgccga tcagttcgcg gaagcgctgc tcgaattcga
     6001 tgatcgccgc atcggcataa ccgtgctgcg gatcgagcag ggcccgcgac atcatggtca
     6061 accggtgacg gatgcaggca taaccttcag gacagtcatc agcgccaggg tgcgggtgca
     6121 gccgcaacca caggttgcgc aggccgggca gggtacgcag cgcgagatgc tccacccggc
     6181 tgtcttcgaa cacctccacc gacagacgct ggaacgggct cagattgtca gccactaccg
     6241 gatgggtcca gcgccggtgc gcagccacgt gggctaccag cgcgcgataa cggtccacgc
     6301 cgcgcacacc ggcgatatcg tcgtacactt ccggcacatg caggccattg gcgtcgtagt
     6361 acggcaccgg ccggcgcagg gtatcgaatg ccagcgaata tggcacgaac tgcgcttcac
     6421 tgtgccagag cccgcgcagg tacagatcga gccgatgctc gatgtcgatg aacagtgtgc
     6481 cgtggcgctc gcgttgcacc acagcccgac tgtcgggcgt gtcgaggcgg aagtattccc
     6541 gttgccggtc gggatcgccg gcataagcgc gaataccgta ctccacccag ttgcgcaggc
     6601 cggcaaacga cagcatgccg aacagccgcg gcacgctgcc cagcatttcg accagacagg
     6661 cgctggggta catgttgtcg agaccgtgca cctggggact ggtgcgctgc agggtagacc
     6721 acaccagatg gaaatattcc cgcaataacg gcaggctttc catgctgcgc gctgcagctg
     6781 ccaggctttg caggaacggt gcaatcgccg gtccgttggg agtgcgcgtc aaccgctgca
     6841 caaaggcgac ggtttcgcca atcgcggcct cgccaagtcc ggctgccaca ccgggcattt
     6901 cctccatgaa cgccagcaca ggcgcatctc cgcgccccat gcgacacagc aactgcgccc
     6961 catccagcca gtgctgcacg ccctcggccg acagcagccg gctggcgttg gcgtggcagt
     7021 cggcgaatac gctttgcaca ctggcaaagc cgcaggcaag cacacgcgtt tcgacgtcct
     7081 tcgcgtcctt catcgccgca tcccgttcag gcgaacacgg aatcgatgct gtggttgagc
     7141 gtgtcgcaga tatcggcgtc gtcgctgatc gggcgcacca gcgccatgcg acaggcgtcg
     7201 cgtgctacga tcccccgctg catcagggtg cctgcataca ccagcaaccg ggtggaaatg
     7261 ccctcgtcga ggccatgtcc cttgagcgca cgcgccacct cggccacctg gaccagtcgc
     7321 tcggcgacgt cttcacccac gccgctttcc ttgctgacga tggacgcctc caccgaacgc
     7381 tgcggatagt cgaattcgag tgcagcaaaa cgctgtttgg tagattgctt caggtctttc
     7441 atcaggctct gatagcctgg attatacgaa atcaccagct ggaagtcggg gtgagcgctc
     7501 accagctccc ccttcttgtc gagcggcaat gtgcggcggt ggtcggtcag tggatggatg
     7561 actaccgtcg tatcctggcg cgcctcgacc acttcatcca gatagcagat cgcaccatga
     7621 cgcgctgcca gagtcagcgg accatcgagc cagcgcgtgc cacccgcatc cagcaggtaa
     7681 cggccgacaa ggtcggaggc ggtcatgtct tcgttgcacg ccacggtgac cagcggcttg
     7741 cccagcttcc acgccatgta ttcgatgaaa cgcgatttgc cgcagccagt cggccccttc
     7801 accatcaccg gcagacgggc ggcataggcg gcctcataca aggccacttc attgtgctgc
     7861 ggcagataga atggttgctg tgcgatacgg tattgtccaa tgtcgaaatg gctgctcatc
     7921 ttgccctcac gctatgcatg gatatgttct gcggcggtaa aaaagcccat gccttgacat
     7981 gggcttttca gccgggccgc cacttgcagc ggccccgaag cgcgcaggat tacttgtgca
     8041 cgcccagttt ttcacgccag cccgggaaga tcttgtcagc gtcgttgggg aacgattcga
     8101 acgcacgggc gaattcgcgg tgttccttgg cgaactcgat cgggtcggcg ccggacttcc
     8161 agcactcata cgactgacgc agcgagatgg cgccagcagc cgggctgtcg atgtggccat
     8221 acgaaccgcc acccgcggtg ttgatgacgt tgccgtgacc gaggttctcg aagaaacccg
     8281 gcagacgcag cgcgttcatg ccaccggaga tgatcggggt ggtcggcttc atgccatacc
     8341 acttctggaa gtagaccggg ccctggcact cgtcgcgctc gatcatgtaa gcgatgatct
     8401 tgtcgctcat gtcaccttcc atcttgccgt agcccatggt gccgacgtgg atgccggatg
     8461 cgccctgaag acgcgacatc ttggccagca cgaacgcggt gtaaccgcgc ttggcggatg
     8521 gcgaggtcac ggcaccgtga ccggcgcggt ggtagtgcag atattggttc gggtactgac
     8581 ggcgagccgt ggtcaccata ccggggccgc ccacataccc gtcaaccagg aaagccacct
     8641 tgtcggcatc cggcccgaac gtttccagga tgtagtcggc gcgggcgcac atttcgtgat
     8701 ggtcgtcagc ggtgatgttg gccgagaaca gcttggcctg gccggtttcg tcctgagcgc
     8761 gcttgagcgc gtcggccacc agcggaatca ctttcttcat cggacagaac gtctggttgc
     8821 cctgcggttc atcattcttg atgaagtcgc cacccagcca gaactgatag gctgccttgg
     8881 caaacggctc gggacgcaga cccagcttgg gcttgatgat ggttccggcg atgtagccgc
     8941 catcctggac tggccggcca aggatgcgcc acaggtcgct gatgtcgcgc gaggggccgt
     9001 cgaacaactg gatggcgcgt tccggcacgt ggaagtcaat catcttggca tgctcgatgt
     9061 cgcccatgcc ctggttgtta ccgatggtca gggtcaggaa cgacaccagc atcatgcggc
     9121 catcgatgac gttacggtcg aacaggtcga gcggataggc aatccgcatg tcttcgttgg
     9181 cttcgtcgat gtaatacacc agcgcatcca ccccgcgggt aaagtcatcg gtggtggaca
     9241 cttcgacgtt ggtgcccgtg gaagactcgg cggcaaagtg agccgccgct tccagataac
     9301 cgtgaccaga cttcggcttc atcttgtagg ccaccaggat atgcttgccg ttcttgatga
     9361 gttcgtcttc cttcagactc agatccgagt aacgtgcgga ttgatccatc gtcattctcc
     9421 agtcaatata aaaaatggtc ccgtaacttc cggcgctgcc agatggcggg ttgtaaccgt
     9481 aaaaccataa tggttttctg caatgcccgc aactatagac tcgttagtct ataaggtaaa
     9541 ttcaattgtt ttaataatat gtataagcaa aacattatgc atttattgca gaaacggcaa
     9601 attgcagcaa catgggaacg cacgggcccg accattgcga aaatcgcagg caacagcggg
     9661 cccccgggcg catgatgcgc caggtcaggc aacggatacg aacggaagaa aaagaaagaa
     9721 acgtcagttc ttgagcagca tggcccagtt gatggcgtcg atatgaatct ggttcagcgc
     9781 ctgcgcgtcg atggcgagcg cgtcggccag ttcgcgcacc tgctgctggt cggcattttc
     9841 gaaaccgatg gccagccgca ggaaaggcgc atacgggccg tgttgctgca ccagcgcccg
     9901 ccgcgcattc tccgacagat tgagccgttc gatggcctgt tccatcggca ggttgaacaa
     9961 tacatccagc agggaaaaca tgccgatcac gaacagggca tcggcatctg ccggagtgag
    10021 gtacttgcgg ccgagcgact cagccatgcg cgcacgcacc aaggcattct gcagtagcgc
    10081 catgtcgcgc gcttcctact cgccatcggc gaacagcagc agcgtcagcc agcgatagag
    10141 cgggtcgtag cccagaaaca tcagcgcctg ggcaatggat tccattttct tgctcagggc
    10201 gttgagcggc gaattgatga atgccagcaa cttgtacgaa atgagcacat cgtgcttgat
    10261 cgcctgctcc agttctgcaa gttcggcatg ctgggcggtc atgttgagca attccatgat
    10321 gcgcagacga tcattgtcga tacgttgcgg cagggcagga ttacgccgag cgaagtaata
    10381 accctggaag gcatcgaaac ccatgccgcg cgcggcctgg tgatcgtctt catcctcgac
    10441 ctgacgcgca acgaccacgg ccttgcaatg cgcacgcaat tcgtccagtt gccgattgag
    10501 atcgatggcg ttgtagcggc gcaggtcgaa acgcagataa gctgccagac cggccagctc
    10561 gcgcatgcct tcgccctcgg ccagattgtc gaacgcaatg cgaaacccct gttcgatacg
    10621 cgcacggcag cgttcggcca gttcggccac gggccccggc tggctggcgt cgatcgacag
    10681 caccgccagc tcgggcggta gcatactcac ccatccgctg tcgagcagat cagcggcgat
    10741 cggcagaaaa aacattttgt cgccggtcag tgagtggata ttgagctgaa cgacactgga
    10801 aatcagcgtc tcgtccgaca gctgctgcat cgcatctgac gatgatagtt cgtggcgcgg
    10861 acggaaggca aattcataac cgatgatctg ctgcgacacg ttgagcaccg gctggcgcag
    10921 caagaaacgg cctatgtctt ccggcaacgg ctgatgctcc tcatctgccg gtgtcaatcc
    10981 gacgatgtca tcggtggcgg gagcaggagc agccaccagc ccggtttccg gccgaacggg
    11041 tgcggcgggt tgcaccgtgg caggggtctt gcgtgcgaac ttgaaccagc gatcgaacat
    11101 gggcttcgtc tcaaatcgaa cctacaatca aacgcagagc gcgcgtacgt gtccggtttc
    11161 gccacgcctg tgcgccactg cgcggctgcc cgtcatggaa ttatgccaca agcccgccgc
    11221 gcatacgtcc aggctggcca gcattgcgtc aatcctgcgg caggttgtcc agcgtggcgg
    11281 cccaggtcat ggcttccacc agcagcggat tgacgcgatc ggcattcagt ccaaggcttc
    11341 gcgccagcgc gtcggcatcg taccactgcc ctccttcgag cgcgatgccc agtttcaggt
    11401 agggtgtgta cggcccttcg ccggacaaca gcgcctcgcg aatcgctccg gtcaggttga
    11461 gcggctcgat ggcctcttcc atcggggtat tgagcaacac atcaagcagt gagaacatgc
    11521 ccagcagaaa cagcgcttcg gcgtccttgc gtggcagatg caccagcccg atctgttcga
    11581 gcaggcgacc gcgcagcagc gcctgctgca acagcaaccg gtcgcgtgga tggcgcgtgc
    11641 cgcaggtaaa cagcagcacc gtcaaccagc ggtagagcgg gtcgtaacca agaaacatca
    11701 atgcctgggc aattgactcc agctggtggt ccagcccgct cagaggcgag ttgatgtaca
    11761 ccagcagctt gtatgccagc atggcatcgc ggcgcaacac cgagtcgagc tcgctgattt
    11821 cggcacgctg cgccaccagg ttgagcagcc gcatcacgcg aatgcggtca tgatcgacgc
    11881 ggcgcgccat ctgcgggcgc atgcgcgcaa aatagtagcc ctggaacgcg tcgaacgtca
    11941 tgacctgaca ggcctcgaaa tcgtcctcgg tttctacctg acgggcaacc agcatcagct
    12001 tgccccgttc gcgcaacgac ttgatctggc gatccagctc caccgcgttg tagctgcgca
    12061 gatcaaagcg caagaaggcc agcgacgaca acagggaacc gtcggccgga ccatcgggaa
    12121 gattgtcgaa cgcaatgcgc cagccttctg acgcacgggc ctgcacgcct gcgcgcgcct
    12181 gttcgatcac gccgggaaac gacacatcaa tggcaaggac cacccggtct tttggcaggg
    12241 atgccagccg ctcgctctca agcagacggg ggccgatgcg gacgaacagc agaatgtcat
    12301 tgcgcaactg cgacaggtca agcgcagcaa aactggccag cgtttcatcg tcgagcaccc
    12361 ggaccaccgc atccgtgcca tgcgatacgc tggcgtcgcg gtagcccagt tcatggccga
    12421 tgacccggtt cgaggcatca attaccggct gccgcaacaa aaatcccgtc acggactcca
    12481 cacgggacgc cccttccgtt tcgtcgcgct gcagcaacga cctccccccc gcagtggatt
    12541 ccgtactccc cgctgaccag ccaaaccagt ttttgaacat atccacccgc cgtatcagtt
    12601 gcaaggaata cacggccggc accgactcac gatccgtcag gccccgcccg gccttatccc
    12661 ctgcacaagc ttttacggca aagatacacc aaaatcataa ga
//
LOCUS       DAIANL010000041        10900 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_649393, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000041 DAIANL010000000
VERSION     DAIANL010000041.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649393"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2406 a         3615 c         3065 g         1814 t
ORIGIN      
        1 ctgcttcttc ttggcagcgt aggtcagggc ggcaaaagtg ttctgtttca ttgggttaag
       61 tcaggcaaag gttatggcga aattttaaca gattggcgat tcgttcagag attccctaga
      121 ggtctacccc gctataactc aggttgaacg atttgttgat gagcggaaaa tccggcacga
      181 tattgcccat gatggcatgt tccagcaccg gccagttctg ccaggaggga tcgtgcgcat
      241 gcagacgccg gatgcgcccg tccgcatcgg tgtgcagcgc caccatcact tcgccgcgcc
      301 agccttccac ccagccaatc cccggcccgt ccgccacggg tgcacccagc gaaccggcaa
      361 tcgcgcccga tggcatcttg tgcaggatcg aacggatcag ccgcatcgat tccgtcgctt
      421 cttcgaaacg cacggtcacc cgcgccgcca catccccgtt gcgatgtgtg accataccga
      481 catcgagctg atcgtacggc tgacagggga actgcacgcg cagatcccat gcctgcgcac
      541 tggcacgccc cgccagcccg gtcagcccaa gctgcgcggc cagttcgggc gggacgcgcc
      601 cggtagtaag gaagcggtcc tgtgccccgg cgtgctcatc ataaatcgat ttcaaccgtg
      661 ccatctccgc cgagacatgc aacagcgtct cgatgagtcg ggtatgcgcc atcgcatcaa
      721 catccacggc caccccaccg ggtatgacgc aatccatcag gtaacggtgg ccgaacaatt
      781 cgccgttcat gcgcaacaca tcttcccgca gcatgccaaa ctgagccaga ccaaaggcca
      841 gtccggcatc gttgcccagc gcccccaggt cacccagatg attatgcagc cgttccagtt
      901 caagccacag cgcgcgcagc cactgcgccc gcgccggcac tgtggtgccg gtgagcccct
      961 ccgccgcctg ggcatacgcc caggcatatg ccaccgtgct gtcgccggac acccgaccgg
     1021 ccagcctggc gccttgctgc agcgtcagtc cttcggcacg tttctcaata cctttgtgcg
     1081 cgtagcccag ccgttcctcc agccgcagca ctttctcgcc caccaccgaa aagcggaaat
     1141 gacccggttc gatggtgccc gcatgcactg gaccgacggc aatttcatgc acgccgccac
     1201 cctgaaccgt aacgaacggg tagtcatctg tgccggcagc gccgcgaagc gtcacgtcga
     1261 actcctgccg caacggataa gtgtctgcag gccaggccgc atgccgcagc catttacgtg
     1321 catccgcgcc tgcggcgctc acacccagca ggtcagccaa tgcacgttgc atgcgccccg
     1381 ctgccggaaa cacatccgac agatcgggaa accgggcatc ggcctgcgcc accggcagca
     1441 ccatcaccag cacaccttcg gcaaacacca gcgcggcatg cagcaggaag tttccgctgc
     1501 gcgcacggtc atcgattccc cacaacgcca gcaacttgcc gccagcgccg cgcacctcct
     1561 cgcccagact gcgccactcg gcggcgtgta ccgtcagctt ccgcaccggc atcggaccgg
     1621 acaccgtaac cagcctgggt ttatagtcat cgagcggcat catgctatcc aatcagcgca
     1681 gcggcctggt gataccactg cgcgagataa ggcggcacat acaccccgag catcagcacc
     1741 agtccgaggt ggacaaaagt gggcaccagt gccggcgggt gaggcagacg ctgcccggta
     1801 gtctcgccga acaccatcac ctgcatgcgt gaaaaaatgg cggcaaacgc cacacccaga
     1861 gccaacagca aaaccggtgt aatccatgga taggtatgca tggtggtgac cagaatgaga
     1921 aattcgctgg taaacacgcc aaacgggggc atgcccagga tcgccagcgt accgagcgac
     1981 aacccccacc caaccgtggg gctggtcttg atcagtccac gaatctgctc catgatctgg
     2041 ctgcctgact tctgcgccgc atgacccacg gcaaaaaaga tcgccgactt cgtcaggcag
     2101 tgcaccgtca tgtgcagtag cgcggcaaac gtcgccacag cgccgcccat gccgaacgca
     2161 aaagtcatca gccccatgtg ctcgatcgac gaataggcga acatgcgttt gacatccttc
     2221 tgccgcgaca ggaagaatgc cgccatcacc acgctcaaca ggccaaaccc catcatcagg
     2281 ttcccggcga aatgcgtccc cagcgcgcca tccaccagga ctttggagcg caccacggca
     2341 tacagcgcca cattgagcag caagccggaa aggacggcgg aaaccggcgt cggtccttcc
     2401 gcatgcgcgt ccggcagcca gttgtgcaac ggcgccagac caaccttggt gccatagccc
     2461 accagcagga atacgaacgc cagcgacaac accgtaggct cgagctggcc cttgacctga
     2521 ttgaggtgcg tccacaacag cgccgtcccg ttggcaccga gcagccgttc cgcagcaaaa
     2581 tacaacagga tggtgccaaa cagcgcctgg gcaatgccca cgccgcacag gatgaaatac
     2641 ttccatgccg cttccagcga agccggcgtc cgatacaggg acaccagcag caccgtggtc
     2701 agcgtggccg cctccatcgc cacccacagg attcccatat tgttggtcag caatgccagc
     2761 agcatggtaa agcagaacag ctggtacatg ctgtagtaca agcgcagcat gttcgccgag
     2821 agcttgccat ggtgctgctc gatacgcatg tatggccgtg agaacagcga ggtggtgaat
     2881 ccgacgaatg cagtcagcgc cacaaggaac acattgaacg catccacgaa aaactcttcg
     2941 tccagcgccg tgaacgaccc gtgttcaatc acacgccatg tcagcacccc tgccgagatc
     3001 agcgtcagca gactcatcgc ggaattcagc tcgggcgcca ggcggtgctt gccaaacaac
     3061 gccatgacga tgccgccaca caagggaaac aggatcaccc agagaatttc cacctctaat
     3121 cgtccttcag ctgttccatg tgcttcagat caagactgtc gaatgcgaca cggatctgga
     3181 aaaagaaaat gccgaggatg aacatcgcaa tcagcacatc cagggcaatg cccagttcaa
     3241 ccaccatcgg catgccatac gtcgccgaag ttgctgcgaa aaacaggcca ttttccatgg
     3301 caagaaagcc caccacctgc ggtacggcct tgctgcgcgt aatcatcatc aggaatgaca
     3361 acagtacgct ggccatggcg acacccaacg tgctgcgcgt gatggtgcca gccatatgcg
     3421 aaatcggcgc agccagattg aaggcaaaca ccaccagcgc cagcccgacc agcatgatgg
     3481 tcggaatgtt gaacagcgtc tccacatccc agagaatatc cagccgccgt accagcttgt
     3541 gcagaatcac cggcaatatg attcccttca gcaccagcgt gagtccggca gaaaaataca
     3601 ggtggctctg gtgggaagaa tacgctacca ccgtggtgct gaacgcaaga atcagcccct
     3661 gcatggcgaa caggtgcacc agtgacaaca cccggcgctg agacagcatg gcgaatgcaa
     3721 tcagcagcag caatgaggcg cacatgttga tcaattgtcc ggtaaacgga atgtgcgtca
     3781 tgccgcctcc agcaggaaat gggtcagcag gccaagcacg gcaaacagga acgccgtgcc
     3841 catgaattcg gggacacgaa aaatgcgcat cttggccgaa agggtttcga tcagcgcaag
     3901 ccccgcccca gccagcgcca gcttcagcac cagcagcacc acggccagcg gcagcgcact
     3961 ccagtcgcct ggggctgcga tgccatacgg cacgaacagt gcgataccca gtgtcatgta
     4021 tgccagcatt ttcagcgccc cggcccattc gaccagcgcc aggtggcgcg cggaatactc
     4081 caggatcatg gcctcatgta tcatcgtcag ctcaaggtgg gtggccggat tgtccaccgg
     4141 aatgcgcgcg ttttccgcca gcagcaccat gacgaacgcc actgccgcaa aggtcatgct
     4201 cgggtagagc acgaaatgct gctgcgccag cgtttccacg atggtggtca gcgaagtcga
     4261 gtggctgatg gtcgaggcgg tgaacagcac catgagcaac gccggttccg ccaggaagcc
     4321 taccagcatc tcgcgccgcg cgcccatcga gccgaatgcc gtgccaacat ccattgccgc
     4381 cagtgaaatg aacatccgcg cgagggcgaa aatgcctacc agcccgatca cgtccactgc
     4441 gggcgccagc ggcagcccgg tgaccagcac cggcaccagc gccaccgcga gccacatgca
     4501 tgaaaacagc acgtatggca tgacgcgaaa cagcaccgag gcctgatcgg ccagcaccgc
     4561 atccttgtga aacagcttga gcagcatgcg gtaaggctgc agcacgccag cgccgttgcg
     4621 gttttgcagc cacgcccggc actggctgat ccagcccggc agtagcggcg ccgcaaccag
     4681 caacacggta ttctgcacca actggaacag gaggtctgtg atcttcattg cacgaacgcc
     4741 agcaacacca gcagggtgac aaaactgtac aacaggtaca catggatacg tccctgctgc
     4801 aaccggccga tctgtgcgct caacatctcg ctggcgcgct gcaccggcac atagcacaaa
     4861 tcccacaacc ggtcgtgcac actgctgtga aatgtcggcg tcacgtcaaa tggtgaaggc
     4921 atgtgcttca ccacacccat gaaccattcg aatatctgcc ggatcggctg gccaaaacct
     4981 tccgcagtgt cctgcatgcg cgcattctgt tcgggaaacc cgcaatccca tgccggcgcg
     5041 atgcgcaggc gcccatggta gaagcgccgg accatccagg ccgtgaacag cacccccgtg
     5101 ccgatgacca gcaacagaac cagtggtccg taactcgccc gctcggcacg catcggcgtc
     5161 agcagcagcc agcctgtcga gcggacattg cccgccaggg aatggcccac cagttcggct
     5221 gccaccgggt cgatacccag cacgacgaat gtcggcagcg tgcccagcag cacacaaccc
     5281 agcgcgagat atgccatgcc cagctgttca cgcgcaccgg catcgtgcgg cggcacggcg
     5341 gccagtgccc gcgaccgacc aagaaacacc accccataga atttcaccat cacgaagccc
     5401 gccagcgctg ccgccaaagc cagcgctgcc gcagcaaccg gtaccagcat gttgagatag
     5461 ggttgcggca gccccggcga cagcagaaat gcctgcagcg tcaaccattc ggacacgaaa
     5521 ccgttgagcg gtggcaagcc ggccagtgcc aggacaccca ccagcatcag tagcgccagc
     5581 cgcggcatgc catgaatcag cccgcccagc cgacccaggt ggcgctcgcc ggtggcatgc
     5641 agcacggaac cggtgcccag aaacagcaac cctttcatga aggcatgatt cagcgcatgg
     5701 tacagcgccg cggtcaaggc cagcgcagcc agcgcgccct tcccggaagt atggaaaatg
     5761 atggccagac caatgccgat caggatgatg ccgatgttct cgatcgagga ataggccagc
     5821 aaccgtttca tatccacctg tacggcggcg aagatcacgc cgaacagggc tgtgatcagt
     5881 ccgagcgtga gcaacagcac accccaccac cactgcggta cgccaagcag atcgaaactg
     5941 atacgcagca ggccatagac cgccgtcttg agcatgatgc cgctcatcag ggccgacact
     6001 ggcgacggcg ctgcgggatg cgcctccggc aaccaggcat gcaacggcaa tacgccagcc
     6061 ttggcgccaa agcccaccag cgcaagcagc atcgccacgc tggccgacgt gctggtcagg
     6121 tgcacatggc gcatggcggc aaagctgtca tctccatgcg cacccagcat cacgccaaaa
     6181 ctcatcagga tcgccaacgc cccgacatgc gccatcagca aatacaggaa accggcctgg
     6241 cgcacctccg gctgttcatg gtcggtggtc acgagaaagt acgacgacaa ggccatggtc
     6301 tcccacgcca gcatgaacag ataggcgtcg tctgccaaca gcaccgtcgc catgctggcc
     6361 agaaaaacat ggtattccag acacagcagg ccgagtttgc ttacaggcat ggcacgaaaa
     6421 tagccactgg agaacacgct gatgccgacg ctggctgcgc cgagcagcaa caggaaaaag
     6481 gccgacagcg catcgagccg caggtgaaac ggcaggttcg gcaacccgag cggcgctgtc
     6541 gccacctgcg tctgggccgt gccagcgagt gcggcgagcg ccagtgccag cgcggcgacg
     6601 gcgccgagcg ggaatacgat ctgcacgatg aagcgcggat tgtgcgagag caggccagcc
     6661 agcccgagca gcagccagaa cagaatgatc gccatcatcc agtcgatcgg cagcatcccg
     6721 gccagcgtcg acatgatggc tactgccccg gtttgcgatg cggcatgcgc gccgcctggt
     6781 ccgcggtgcc atgctgctgc acataatcgc ggatcagctg acgcaccacc tgcgacggag
     6841 tcaggtcctg cctggcgcag acctcttcga aaatgccttt cttgacagga tcgatcagca
     6901 gcgtcaggcg cgcggtacgg ttctccatgc cataacccgg atatgatgtt aatgacatgt
     6961 taatgccact cacatcaaat tgcaattacc cgcgcggccc gacccgcgcc attgcaggca
     7021 ggcgtgctac actacgcgct gttttcagag cgaactgcgt catgagccat tatcgatacc
     7081 acgtgttctt ctgcactcac gaacgcgaga ccgggcaggc ggcctgccgc ggcagtgcca
     7141 tgcgcgaata tgccaaacgc cgcgtggcct cgctgggact cgatgccgac ggtcgctgcc
     7201 gcatcaacag cgccggttgc ctcggtcgct gcgaaaaagg ccccctgctg gtcatttatc
     7261 cgcaggaggt ctggtacacc tatgtggacg aaacagatat cgacgagatc atcgaacacc
     7321 acctgctcgg cgaccagatc gtcgatcggc tcagacagga atgaaacgcg cacggcttcg
     7381 cataccttcc agcgccgggt tcatcgaaac cctggtcaac gatcccgggt cggagcggcg
     7441 tggcctggta ttcatcgccc atccccaccc gctgcatggc ggcaccctgg acaacaaagt
     7501 ggtgcaaagc atcgcccagt tatgccacga acaaggcatg gtggccgtac ggcccaattt
     7561 ccgtggcgtc ggacaaagtg acggcacata caccggtggc gacggcgaaa ccgacgacat
     7621 gctgtgcgtc atcgaatttg tccgggccca ttacgacacg accctgccga tcctgctggc
     7681 cggattctcc tttggcggtt acgtccagca ccgcgtggca caacaactgg cgccgcagcg
     7741 cctgttgctc atcagcccgg cggtcaacct gtatgatttc ggtgaattgc cggcgcagac
     7801 caccatcatc cacggagaac aggacgaaat cgtgcctttc gacgcggcat ggaaatttgc
     7861 cctggcgcaa cgcaccgaat tccgcccgat cgccggtgcc ggccatttct atcatggcaa
     7921 gctcggtgaa ctgaaactgg aggcgctcgc agcatgccac cgctcgtaag cattcgctcg
     7981 cttggcaaac ggtacggcgc caccgaagtc gtgcgcgggc tcgatatcga catcggcgcc
     8041 ggcgaatgct tcggcctgct cggcccgaac ggtgccggca aaaccacgac gttgcgcatg
     8101 ctgctcggtc tggtccagcc ggatgccgga gaaatccgca tcggcggcat ggcgatcccg
     8161 cgcgaggcgc gcgaggcgcg ccagcgcatt ggcgtcatgc cacaaatgga caacctcgac
     8221 cccgatttca gcgccgccga gaacctgctg gtgtacggcc gctatttcgg cctgaacgac
     8281 ctgaccatcc gcgcacgcat tccagcgctg ctcgaatttg ccggactgtc cggcaaggcc
     8341 gacagcccga tcgccacact gtccggcggc atgaaacgcc ggttgacgct cgcgcgcgcc
     8401 ctgatcaatg acccggaact gatcgtgctc gacgagccga ccaccggcct cgacccgcag
     8461 gcgcgccacg tcatctggca agggttgcgc cggctgacac agcagggcaa gaccattctg
     8521 ctcaccactc atttcatgga agaagccgaa cggctgtgcg atcgtctcac catcgtcgac
     8581 cacggccaga ccattgccag cggccggccg cgcgacctga tcgatcgcga aatcgaaccg
     8641 ttcgtcaccg aggtgttcgg tgaatcggtg gcgcagtggg ccgaaaccca tggctcgtgc
     8701 tgcgaacggc tcgaactctc gggcgaatcg ctctactgct acggccgcga cccggaccgg
     8761 gtgcgcgcgg cactgcagca tgcgcctgac ctgcattatg tacagcgccc ttccaacctg
     8821 gaggatgtgt ttctcaaact gaccggaagg gagttgcgcg acgcatgaac gcctttgccc
     8881 caccccgcct gtccctgcgt tttctgcccg tgtggcggcg acatttcctc gtctggcgca
     8941 aactcgccat tcccagcatt ctgggcaatc tggccgaccc gatgttctac atgctcggct
     9001 tcggctacgg cctgggcagc ctcatgccgc gcatcgacgg tgcaagttac ctcacctttc
     9061 tctcggccgg caccctctgc tattccacca tgaacagcgc cagcttcgaa gtgctctact
     9121 ccggcttctc gcgcatgcat gtgcagaaaa cctgggaagc catgctcaac gcaccactgg
     9181 cactggatga cgtgctgctg gccgaactgg cctggggcgc gtcgaaaagc atgctgtccg
     9241 gactggccat ccttgccgtg atctggacgc tcggactgga acacgactgg acaatgagcc
     9301 tgtggacaat cccgctggtg ctgctgatcg gactcacgtt ttccgccatg gggctggtgg
     9361 tcaccgcgtt gtcgcccagc tacgattttt tcatgtacta cttcacgctg gccatcacac
     9421 cgatggtact gctgtgcggc gtgttctacc cgatggaacg catgccgcaa ctgttgcagg
     9481 ttgtggccat gtttttgccg ctcagccatg ccatcgcact ggcacggcca ctcatcctcg
     9541 gcacgatccc cacggccatc ggctggcatg tactcaatct gctgctgtgg accggcgcca
     9601 gctactggct ggccaccgga ttcacccgcc gccgcctgct gagctgacgt ccgcagcggc
     9661 acggggtacc gaaaaatcga acaggttcat cagatcgccg atggccggcc ggttgagcgg
     9721 cacgtagggc gaggccgcac tgtgctgcgg attgggcagg ttgtcacggc tgcggcccga
     9781 caacggaaac aggccccagt tgtgttcgat gaatttcaga atcgagacat gatcataata
     9841 ggtatggtcg acataccccg tcttcaccca tggcgaaacg gcaatcagcg gaatgcgcgt
     9901 accgtcgccg aaaaaatcga tcggctggat gtatcccgag tcataatacc cgcccccttc
     9961 atcgaacgtg atcaggatga ctgtattgcg ccacagttcc ggattgctgc gcactttatc
    10021 gacgatgtcg gccacgaaac catcgtagct cggctccatc gcatatcccg ggtggccgga
    10081 cacactgtca taaggcgtga tgaacgccac cgcgggaaaa tgcccggcat ccttcacatc
    10141 gcggtagaaa tccgcaccat cgaccagatt ctgccgaagc accccgttca tgacggtgcg
    10201 cgaataggta aatggatcac agatcgtgca atattccttg tccggatgct ggccatcgcc
    10261 gcgacctccg gcataccatt tccatgacac ccctttctcg gacagcgcct gaccgatgtt
    10321 gggcgtctct tgcggcggca cgacgaaacg atcggggccg agcaccgccg gcacgccagc
    10381 ggacgaatac cccggctcat aattgttcac cagataatac gtgccgggcg cacagttgcc
    10441 atggcgaaac ggtatgtacg gcagccggtc gagatactgg tacaccgcag ccacgcccgg
    10501 ctgatcccgg tcagcgcagg caacgtagga accaccgcgg tagccatcat gccggtacca
    10561 gttatcgctg ccaggcaacg gctcgggatt ctcgatctgg ttggccggtg gccgcgccgg
    10621 ctggccatcc agcacatagc tcgccacgtc gccggtggca agtgcataga aattggggcc
    10681 ggtacccccc atgacgggct ggtgataatt gtccgcaatt gcaaatcgct ccgcaagcgt
    10741 gcggaaatac ggcgcatcgc cagactgcat gttgtagaag cccatggcca ccccaccctg
    10801 ataggtgtgg ccaggcacaa ggccaggcac gccattctcg ccaccgaagc caatggtttc
    10861 gcccacccag acaaacagat cctgtcggcc tccgtcgtac
//
LOCUS       DAIANL010000042        18739 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2896693, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000042 DAIANL010000000
VERSION     DAIANL010000042.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 18739)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18739)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18739
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2896693"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3472 a         5230 c         6130 g         3907 t
ORIGIN      
        1 cagcgtcggc cgcacgtcgg aaccagcagg gcgtcacgct gatcattgca ttgctgctgc
       61 tggtgatcct gtcactggcc ggcctgtcag tggcccggat gtcgacgatt gcccagcgcg
      121 tcagtggcga tcttgcgcag acgcagatgc tgaccgcgag caacgatctg gcgttgatgc
      181 tggtcaagca gcaactggcg gcggtgccgg ggaacgtggc aatcacaggc aaccgtcaac
      241 cctggtacaa caccggcaac gtggttccgg atgcggtgtt ctggcagacc tgccaacctc
      301 atggtggtac gagtgccagt tgcgccagca gccagaccac cgtgggcaac cagacagtga
      361 ctgtccagta catggtgcgc gccaccccat ttacccaatc attgggaacg gtgacgtcgc
      421 aggggcagca gcttaacggc agctattacc aggtgtttac ccacgcatcg gcgacggacg
      481 gcaccgtggc ggatgccgag gcctggtatc tcaaatcctg aaggagacac atcatgcccc
      541 ggtcgtcctg cagcaaggct tgtgcacgca aattcgtcgc gggactgatt gcgtggtgtc
      601 tggcggtgca gccgtcggcc gctgctgccg gcagtccggc cacggtcatg gtcgctccga
      661 acctgatgat cgtgttgagc acctcgtatt cgatgaaccg gctgctgagc gataccggat
      721 acccggcgct ggtaccggcc agtgccagtt cgtccggcaa catgtatgca atgaatgatc
      781 cctacaatcc gggggccggg aatccgtccg ggcatctgga cggtccggtc aacgcctatg
      841 gtgatatgcc cggttcgaaa ctgtaccagg ccaagctggc cttgcagacg atactgcaca
      901 gtccgcaatc ggacaggatc aacgtcggtt ttgcaacgta ccggatgatt tatggtcagg
      961 tgcttgtggc gggaagcatc acgacatcgg ccggcgttgc cgcgcgcccg cagccggccg
     1021 atccggcgat attcagtcag gacacccaga ccaagacgca gtatgcggcg aacccggaac
     1081 actttgtctc ggtatcgtgg tggcctttgc gttacctcgg ttatccggct ggcagcgctt
     1141 gcgacacact ggcgagaagc cagtggccgg caggaaatta catcaacgga ttgagcaatg
     1201 ccggagcggc cctgcttggc aacggcatat gtgcaggtac ggtcgggctg accgatacga
     1261 ctgccgcaca atggctgccc gcagtgcccg atggctatgg ttttgccggt ggtgggctgc
     1321 cgacctgggt acaggccagt ggtggcccgg gctattacct gtgcagcctg agctacaact
     1381 cgcaggccaa tacatttgcg gcgggatatg gcagcgatac ggtgatgagc caggcagagt
     1441 cgtacgtggc cgcgctgggc atggggaatt ttctgtcgcc ggaaggcacg tcgatgcgac
     1501 tgacgaaagg cagtcatgcc ccctgtccgc tggcccagtc caatattgcg gtgcctgcgg
     1561 cagtgacggc ggccatcgcc gccaacctgc cggcccggac cgtgaccaac ccggacggca
     1621 cgcaaaccag catcccggcg ggcgacgcat acggttgggg tcctcaggtg caggccacga
     1681 acgggaatga ctggccatcc gaaggggcag cctacagtgc accggcggat gtcgccggcc
     1741 agattgcagc gtatgcgcgc accggcgtgt atggcgagta tcaggcggga gtgccgggcg
     1801 cgcaggacgg cgtgttgtcg ggctggtctg gcgaaaccag ctacacctgt gtcgatcgga
     1861 atggcgcgcc agcggcgaac cgttgcgtgc agccgccgga tcagggtggc agcgcacaat
     1921 acagcgagca ggtcggagcg cattatccct ccgggccggc agatcccgat ggtccgcaac
     1981 ggcggctgga aacaggtgtg tcgatgttca gcgcgaccgg tctgacccgg cagacggcag
     2041 ccagtctggc cgcaggaggt ggcatgcaga acatggcggc acaactggca tcgcagatcc
     2101 atcacatggg gccgttcgtc gatttgcccg agccgcaggt gggatacgct gaccagcgcg
     2161 tgctcatcga caggctgctg ggtgtggtgc agatgcgcaa cgatggtgcg gattacgatc
     2221 cggccagcca gaccatggcg ccggtcaccc tggccaatgt gtcgggtcgg ccgccgtcgg
     2281 ggcattatgg cgtcagtatc agcggctatg acggagcggc aggcagcccg gtctacgaca
     2341 cgctcgaaga cgcctatgcc tactacaaat cctatgaagc gcaggatccg tacgtcaaat
     2401 gtcgcagcaa caacctgttg ctggtgatcg atggcaaaga ggatgcgcgt tactggatac
     2461 agaacggcca gaccacctac gccagcccgg ccaatgtggc ggcgcggctg gacaaggacc
     2521 tgaacgtcaa ggtatgggtc atcatcatga gcacctccgc gggcgacgtg cagcaggcca
     2581 atgccattgc gcagaatggc ggtaccgggc aggcctatca gaccgccgat ccgaccgcgc
     2641 tgctggcttc gctgggctcg gtgttttccg ccctgtcagg ccagatcaat acccaatccg
     2701 tcgccgcgcc tgtcggctcg catggtaacg gagtgctgta tgccacggcg gacagtttgt
     2761 cgccgacgca aggccatgtg tatgcttatc cgctatccgg taacggtctt ccgggcacgt
     2821 tgccgttgtg ggatgctgcg acgcttgaat cggtgctgat gcgcggcaat gcgctgtggt
     2881 cgaccgggcc gcagtccacc ggtgccgtgg gttcagggct gccggtcttg ctcacacagc
     2941 tcgatgccgc ggcgtttgcg ctcgccccgt catcgccgtt gaccgtgcag tccatcgtcg
     3001 attacaccct caatccgtcc tggccgtgca cggtgaactg tggcagtgac ccggtggcca
     3061 gtccttatct ggacgggcgc gctccgggtg cttacctggg cgaaatgaac ctggtccaga
     3121 gcgcagtgtt gctcgatccg ccctccagca accagttgtt gcgcgaatat ccgtcctatg
     3181 ccgtatgggc gcagggagaa gccagccggc taccgctggt gctggcgtcg tcgcaggatg
     3241 gcttcctgta tgcgttttac caggacggtg atgtctcggg tgcgaccgac ctgtccggtc
     3301 aactggcctg gggctggatg ccgcgcgaat tcgtacagtt cctgcaggat tacacccgtt
     3361 tccccgccag ccattatggt aatggcgcgc tcacggtggt tgacgcgctg gatggcaaca
     3421 accgctgggc gacctatctt gctggtacgc tggcttcggg cgcaacgcat tatgccttgc
     3481 ggctggccgc caatggccgt cccgctgcgg tggtgttcga cgactggcgg ccaggcggcg
     3541 cgtcgatcgc attgaccgca cctgcgacga tcatggctgg cggcacgacc tgcgtgctgt
     3601 acctggtcaa tgccgtgggt cagggtagcg tgacgccgac gctggtcgtg cggcagatcg
     3661 acaccggcgg cgaaccgatg ggcgaggcag cgttgccgtt tacgccggca tcgaacctgt
     3721 ttgtgctcgg cggaatgctg tatgttggcg atgcttccgg caatctatgg tgcgcgccgc
     3781 tgacggacag cagcggcagc cgcctcgtcc cggcaattca cttcagtcag gttggcagtt
     3841 tttacgatgc cagtgcggct ggtgacagcg accatgtgat ccagtttgtc ggcggcatcc
     3901 tgaatggcaa tctgccctgg atctacgcgc aatcgacgag ccgtgtcacg gtgttcaacc
     3961 aggagacggg cggaacctgg ttacgcgact ggaccagctt tgatggtggc gcaggcacat
     4021 ggaatccgca aggcagctac acgccatcga cgcttggcgt ggtgccggca gggcaagccg
     4081 tacccgccac gggcgtgcaa tggctgccgg cggctaccac aacgatggga gccgcgcaga
     4141 tcagtggtaa ttccaccatc gtcagcaaca gtctgattgt gccggtgacc atgacggatg
     4201 cggcgcaggc atgcagcgcc ggcaacgcct acgattacct gttcagcttg cagaacggcc
     4261 agtttccgct cggtgcattc acctttctgc aaccctcgag cgggatgccg gacggcaccg
     4321 cgacaactgc ggcatccgtc accggcaatg tctatatcgg gctcggcacg gcttatgccg
     4381 caacggcgac gcttaacagc gatgtgctcg acgtgtactc ggccagtgag cagagcaatg
     4441 ccgccaatgg ccagacgcac ggtacaggcg cctgcctgac gtgtgcgttt cagtcggctc
     4501 agcctcaggt aagcctgatc tggtggcgca tcctgaacaa ttgacgagga gaaaatcatg
     4561 cgtacgcacc gcgctttcac cttgatagaa ctgctggtga cgatggctgt gctggcgatt
     4621 ctgagtgcta tcgcctggcc caattacctc aattacgtca accagtcgaa ggcagccact
     4681 gcccaacagg cgatcgccac tgccatgggc gcgctcgaac agcagtatct cgacacgcgc
     4741 agctatccgg ccagcgatac gattgccgac acgcacaatt atttcacgta cgtgtacacg
     4801 ccaaccggca atccgcctgg gcagtcgtac accattaccg ccagcggcaa cggcgtgctc
     4861 agccagtatt acatcgccgc cagcagcaat gacctgcgct gtgcctgtct cgcgtgcagc
     4921 ggaaatccgc tcaacgggtt cagcgcctcg ctgaacagtt gccctgcggg cagcgttcca
     4981 tggtgatgat cgggttttta caggatgatt tcaatgctct gagcaggcgc gcggcaagtt
     5041 ccgccgtcac tgcgggtgag gggcgcgggc caggctgtgt ccgtcccccc cgcgggtacc
     5101 gttgtgactg ttgccgggca agcgctcagt agccccagca ggttgacgcg gcggtagttc
     5161 cggtaaccga cttgatgccc agtgaatcga gcgtgaagct accgcaactg tcattggcct
     5221 ggttgccgac aggcacagcc tgcaaggtaa agccattgtt tcccttactg tcggtcagtc
     5281 cttgcacgct caggttgtaa taaccgctca cggaagtggg gtccgcagtg taaccaagcg
     5341 tggtcagact gctcacatag gtattattgg tggtgtagta acttgcctca tggctggcta
     5401 gttcctgaag cgcattgatc gcggccgtac ggtgcgattg caggacatag ttccgatagg
     5461 ccgggaaagc tactgctgcc aggatcgcga cgatgacgat cacgatcagc aattcgatca
     5521 gagtaaaacc agcgtgtttc atcgcaatat ctcccagttt acgcgtttgc ctagatatgg
     5581 attgggcgga ttgaccggcg gtacgttgtt gccgctgcag ttgctttgac actgcggtgt
     5641 ccagattggc ggtatgctgg ctgaaatgga agaactggat gcctgggtga acaggtaggt
     5701 gttgttgttg aaggtcagca gccagggcgt gcctgttccg ttggacagtt caccgttgac
     5761 accgctgctg cctgtaccat aagtgcctcc gctcccctgg aaaaagccgc tgggcagact
     5821 gccgccggtt tggacattga aggccatggt ccagccggtg gttgtcaggg cggaacagct
     5881 gatgacattt ccccctggcg gaatggtggt gttcagtatc agtactccag agctgagcag
     5941 cggattgtag atcacctgtt cgtattgcgt cggggattgg ccgttggttc ccggcaaatc
     6001 gacgtaccag ccgtactggt tgtccgatga tgctggcgaa catgcggatg atccttgcgg
     6061 acaaatggga tagttgctca gtgtgcgcga ggtgccgctg gtgctgatgg tctgctgcaa
     6121 caggttgctg cgggtcaggg cgggtgcgga ggacagcgca gccagctgtg tggccgaagc
     6181 aggcacattt acgccgttgg ccgtggaata gccggtattc cagttggtca tattccagtc
     6241 ccagatgccg taaaccgttt gtgtgccgct ggcgtaactg gctggggtgg atgtggtggc
     6301 tggaatttgt tcgcctgtgc cgaacatgac tatgacatag gtctgaccgg acaggagtgt
     6361 ttcggctacc tggatttgtg tggtgaccgg ttgagtggcg ccgctgccat tctttgcggt
     6421 gtaaagcggg gtggccgtac tgctgccgta tttcgagacg gcccagtcag acggcttgtt
     6481 actggtcagg ttgaatcgcc aaatgttgcc ttgcagatcg ccggcataga cgtagtcggc
     6541 gatgtagtcc ccgtccagat cggctgcggc gacataatcg attccgtccg gattgctgga
     6601 agatccggcg ccggtatcaa ggtaataaaa ggtgactgcg ccgttgtacg gattgatgag
     6661 gccgatgtag atcccggcgt gacctgtgct gctgttgtga ccgttgccga atatcatcgc
     6721 ccattggccg ttgtgcagcc gttcgaagat cggtttcccc cacaattgtc ccaggtagga
     6781 gccgcaggaa gcaatggctc cagcgcaact gatggtggac gggttccact cgcccttgac
     6841 caacgatgcg gcgtttgttt cgctgaaact gctcgggtcg gtcacgtcca gcgcgtaaat
     6901 tgcggaaccg ccggtaccaa gaccgccggc cagccaggtg tgccagccat tgttgtagaa
     6961 tacatccccg ctccccggag tggcatcgac atagtaggca tgcgaataac ccgggttcgt
     7021 cagtgcgtcg acgttattcg tgctgctgct cgccagagtg tcgggaacgt aggcaagcat
     7081 ttccagtccg tcattgggta cggtggcggt gctgtagcaa gcattcggat tggtgctcgt
     7141 gttcggattg cagacaaagc tgccgctgcg gaacccgtgc agcaggccgt cattggcgcc
     7201 atcgtagacg atgttggcgc gaccgccata acctcctgta ccaaacgagc tgaatgtggt
     7261 gtatggcgtg gccccggtgg cattttcggg cagataggtc gaggttccgg agaggccata
     7321 caagccatcg taccagtacg tgccataggg ggcgctcggt atgccgatag cgatcgggct
     7381 ggaatcgatg atatccccca gtacgccggt ccgcgtgcgc agagggccag acgattgttc
     7441 ttcactgcgc accccacgca accagttgag tatgtcctgt ccaccgatcg atgtggaaat
     7501 cagcgctgct tcgttgttaa aggcagtagt tgctgtgctg gacaggttgc cccattcgaa
     7561 tgggacgccg gccccggagc tggatgtcag gatgctgcgg ctggatggcg attccgccgt
     7621 cattgacgtg gctccggtag acgggcagga cccgccggtc aatacgcagc caccatccca
     7681 gtttgcggcg gttgccactt ggaccgttgc gctcgaaccg gttcctgtgc tcgagatgcc
     7741 gatcgccacg aggctgcccc accagttgtc gggataatac gatgccatat agagctgggt
     7801 acccgtattg agctggccgg actgaacgct gttggaggcg gcgccggtgc tggatgacaa
     7861 cggttctgcc ttgaaacagg tgatctcatg tacattgtcg ctgccaccgg tcgacccacc
     7921 gaagccgaac agaaagcttg ctggcatggg gccattgctg gctgcgatac tctggttggt
     7981 catcacgggc tgatacgaac cgccgttgta gtagtaaccg aaggtcagca gcccggtgct
     8041 ggaaatcttc agtgcatagg tgatcggtat cgcattggcg cgcgaagccg tgctggattt
     8101 ttgctggttg gcaataggct ggcagttggg gtagctgctc gtggtggatg gcgtcagcgt
     8161 ggccgccccg gccgtggggc atccgctcag gggtagcgat ttttgtgaat tcgggatgta
     8221 gggatagtct ggaatattgg tgccggtgct tttccaggaa ctgccggaat aagacatgac
     8281 ctgtccgctt ttacatgtgg cctgtacccc actgatcagg gtgctgctcg aataactggt
     8341 cttgtaatag gtcggatagt tgtcctgcag ccagctcagc gcaatattgc cataaccacg
     8401 caggccgatt tcattgggcg atgtggaact gctggtgtaa ccgctggcag agttgtcgga
     8461 cttgttggta aaattgccgt attcgtcgat gcccagcccg agataggcgc cgaccatgcc
     8521 attgtagggt gagttgctgt ttgagcagtc atagccgaga cttccgcccc aggcaccaat
     8581 attggggtcc tgcgtgccat tgaggatata gaaccccatg ccgtccgcgc ccagtgcctt
     8641 ggtgcctccg gcatcgccac cataggtata ggtggtgaag gtgacctgca cgccctggtc
     8701 ggacgggaaa gacgaattga ggaagatggc gccgttctgg tggtagttgg ggtagccatt
     8761 ggtaaagcgc agggcgcctg agccaatcgt gttgctattg ttcgtcagtc cgacctgggt
     8821 ttcgttgccg taataggcta agccattgca tgcgggaata taattcgaag gattggacga
     8881 cgtcgtctgt gttcctgcgg tcaaacaggc cccgttatag gcggtccagg actggctggt
     8941 ggtccctttg gtgaaatctt cctggatggt catgccgtag gcgtggactg aaagcagtcc
     9001 cagcaattcg gcacccagaa tcgatatttt cagcaggttc atgatcgcgt gctccacgaa
     9061 tgatggtcag gaacggtacg ttgccgtcag ctgcaactgg tgccagtggt gttcatgcgg
     9121 atgctgtaga tcgactgcac cacggccatg gcttcgctgt tcccgccggg cgatacggcg
     9181 gtgatctggt aataggccac ctggttctgg cattgagtgc tgcccgtagt accgacaaac
     9241 tgcacgtagt aaatcggata ggctgcgttc aacggggcct cctgcgaata caggttccag
     9301 ttggctggcg gcgatgccga tacgcctgaa ccccaggtga cgctggcagg tatcgtcatt
     9361 gctacgctgc ttggacagat gttcatggcc ggtgcattcg cgacagtcag tggcgtggtg
     9421 catccgcttc ccagcaccag cggcggggag ccatgcagtt gctgagcctg gctcagcaac
     9481 cagtactcgg cataatcgat ggctgcctcg gctgagtccg tggcgcggct tttttcgcgc
     9541 aggtttccgg cgatggatgt gttggtggtg aatccaccaa acatggccag ccccaatagt
     9601 gtcatcaata cgagaaaaat caggctgact accagaatga atccgttttg tttcatagtc
     9661 tagatcccat tcatcacgcc aaaggtgcgt gcaaatgtca cggtggccgg ctggcctgac
     9721 tggccagaca gcggattggt gaatgtgagt gtgatctgca cggtcttgac ctgggtccag
     9781 ttggtgactg aattggccgg caggtactgg tttaccgaac tcaatccggc gaccgggccg
     9841 gtggtgggcg ctgatgcggc cgaaccgatg ccatacagga tggtcatgcc ggacacgcca
     9901 ctgaccaggc tgtaggtctg gttgttttca gtgcaggcca gtgtatttcc gacgatgctg
     9961 aaggtgtcgg tgtatatcgt tccgctgacg gcctggccgc tgcagccttg gccgctaatg
    10021 ccgttgccat aaaaccggac tgtcagcgtg tccgtgccgg cgacagcgcc tgatgtgccg
    10081 aaaatcgact ggccagattg cgtgaagggt gccgatgccg ggaatgatga agagaggatc
    10141 accgatggcg gcacgagatt ggcgacgggg tagttgaaga accctgcctc ctgtatggaa
    10201 tttcccagaa aaaccattga catacgctca ctgtcctgca gtcctgatag cccctggtta
    10261 gtacttcctg ggctgccctg tacgtttttt gccgtcaggc tcatgctgta gaatgcggtg
    10321 gcgaggccga gtgtcaggaa cagcgcgatg gtcattgcga ccatcagctc gaccagtccg
    10381 ataccagctt gtcgttggat gtcgttcatt gcatacctac cagggtcgag tagttgactg
    10441 cgctgaccgt gccgttggaa aacatgccgg gctgtatggt attccatgtc agtgtgatgg
    10501 tgcataccac ctgggtggta gtcgccgggc aagtcacggt ggcggtgcct gagggcaggg
    10561 ttgcggcaat ggccgtgccc cagttgctca ggtcatagcc tgcctgttgt ggaggggtgc
    10621 acgccgcgcc cagacagttt ccgacggtaa ccgttgagcc ggttgcggtc accgtcgggc
    10681 cgttgaccgt cacggtggtg ggtggcgtga ctgcccagaa agacgtgttg ttgcgcatcg
    10741 caactgccat gttggaggcc agcatggcag cctggccctg atattgtgcc cagccggtat
    10801 tttttatggc catcatctgt atgcctacgg tgcccagcac gccaatcagt atgatgagca
    10861 tggacactag cacttccagc atcgagaatc cctgttcgcg tttcatgggc aagcggctcc
    10921 tgtcgaagtg ctggccaggc cactcacgat gctcaggcag ttccggttgc ctatgttatt
    10981 ggcggcgtcg tggatgctgg ccgttccggt catgaattcg taaccggttt gtgcaatgtg
    11041 gattgccgtg ccgcctgtga acgtgtcgcc atgggacagc gccggcgaaa cgcgcaaaac
    11101 gctggaggca gggggtgagt tgaccaggcc ggagttggtg acatcgacat aaacgagacg
    11161 tccggttccc cagtcggagc tgcttgcgcc cacatcactg catgtggcgc catcggatga
    11221 cgagcagacc cacacgggct gcccgcgctt gatcgcctcg ctgcgggcaa aattgatgtc
    11281 cgagagaagc gaaaacattt ctcccttgat gcgggtctgg gttgtcacgc tctgatacat
    11341 ggggatgccg accgccagta ggatggccag gatcgccatc gtcacgagca gttcgatcag
    11401 cgtcattccg ccctgaacct gacgcatgat tccggttgat acggagtttg ttgttgtcat
    11461 ggtttcacca tcggcatgaa tggccactgt acacattacg tattatgcgt cagttcctaa
    11521 gaaccgccat cctgatccga cagacgtgtc gttccggctg atgagtggcg tcatgacagc
    11581 ggttccgatc gtgctgagca tcgggccgat gctgtgtgtc aggagtgcag caatggccct
    11641 gcgttcagcg cttgccggca gcctgatccg gaatcaggcg acaggcagtt gtcccggact
    11701 gttgctgttt ttcagcacga agctggtgtg caccccactc actccgggaa tgcgggtgat
    11761 gcggtgcaac agcagtacct ggtaggcatc catgtcggcc accagcgctt tgagctggta
    11821 gtcggcggcc tgtccggtga tgagcaggca ttcgacgatt tcgggaatgt cgcggatggc
    11881 ggcttcgaat tgctcgaaac gttccggcgt atgctgatcc atcgaaatat gtatcagtgc
    11941 ggtcaggttg agtccgagct tgcgcggttc gaggcgcgca tgataaccgg caatgtagcc
    12001 cgcctcttcc agtgcgcgaa cgcggcgcag acagggagaa ggcgacaggc cgatgcggtc
    12061 ggcgaggtcc tggttgctca gccggccatc ggcttgcagt gcctgcagga ttttgcggtc
    12121 gtaacggtcg agttccatga tatggcgcga ttctgcagaa tgtgacgtga tccgcgacgg
    12181 atcatgccgg gttgatcggt gaacaggggt gtgccggtgt ttcaggtgca agcgctgtat
    12241 tgtgtggcgt gatattggaa acaaattgcc gtaaacagaa ttatgtggca ataataaatc
    12301 aaattcaagt gatctgacgc gtaaatacgc aagcacttgc gcgtcacgct tcgctatcat
    12361 ggtggctgat aattcgtttc cggagcctgc catgatgctg accgacccct cccacaaata
    12421 tgctgcccag ccgcggatcg cgttgccgga tcgtcagtgg cccgagcgtg cattaaccgc
    12481 ggcacccata tggcttagca ccgatctgcg cgatggcaat caggcgctgt tcgagccgat
    12541 ggattcccag cgcaaattgc gtctgtttcg tctgttgtgc gacattggct gcaagcagat
    12601 cgaggtggca tttcctgcgg cgtcgaaaat tgaatatgat ttcgtacgtg agctgatcga
    12661 gggtgaacac gtgccggacg atgtgactat cggtgtgctc acccaggcgc gcgcgccgct
    12721 catcgaacgt acgctccagt cgctgcgcgg ggcgcggcgg gcaatcgtgc atgtgtataa
    12781 cgccacctcg cggccgtttc gcgaaatcgt gtttggcatg ggccgtgacc aggtcgtgcg
    12841 catggccacc gatgccgtgg agcagattcg tgaactgacg gcgcagcaac cggccaccga
    12901 atggctgctt gaatacagcc cggaaacctt cagcgcgact gaaccggagt tcgcactgga
    12961 aatctgtgat gcggtgactg ccgcgtgggg tgcggctgcg gggcgtccgg tcattctcaa
    13021 tctgccagcc acagtggaat gcgcgacacc aaatgtctac gccgaccaga tcgaatggat
    13081 gcatcgccat ctggcgcggc gtgagtacgt ggtgctcagt gtccatgcgc acaacgatcg
    13141 cggtacggcg gtggctgcgg ccgaactggc gctcatggcc ggtgcgcagc gagtggaagg
    13201 ctgcctgttt ggccatggcg agcgtactgg caatgtcgat ctggtcacgc tggcgctcaa
    13261 tctctacagt cagggtatcg cgcccggtct ggatttctcc agcctgcacg ccgtggcacg
    13321 aacagtcgaa gatgtgaccg gactgccggt ggcgccgcgc catccttacg ccggcgatct
    13381 ggtgttcacg gcgttttccg gatcgcatca ggatgccatc cgcaagggca tggcacggca
    13441 gcaggcgcac gagccgtggc aggtacctta cctgccggtc gatccggccg atctgggctg
    13501 cagttacgca tcggtgatcc gcgtcaacag tcagtcgggc aagggtggag tggcctggct
    13561 gctcgaagcg gcgtgcggaa tccgcttgcc acgtcgcctg caaatcgaat ttgctgcaag
    13621 tgtgcaacag catctcgatg aagtcggagg cgagatcgac ggcgagacgc tgtggcagtt
    13681 gtttcgccgc gaatatctca ctgcggagca gccgctgcga cttggcggtt atcagtgtta
    13741 tggcaacgga acccggttgg gcatccgtat gacggtcgac tggcggggct gcaccagtca
    13801 cattgccggt gagggcaacg ggccaatcga tgccgcggtg gctgcgctgc gtgcgttggg
    13861 tatcgaaatg cgcgtgcacc agttcgagag tcatgcgctc agtgcagatg aggcaggtgc
    13921 ggccgcacag gcctgcgtgt tcatcgaggc cgcagtagcc gactcaagtc gtatgtgtta
    13981 cggtgttggc atcgatgcca acagtaccca tgccgccatt tccgctctga caagtgcctg
    14041 taaccggttg catgcggtta tgagtgaccg cgaggtgcct actggcgtgg tgggcaaggc
    14101 ggagggtaga atgatggcgt gaatcggcag ggctggtggc gtacagccgg ctggaatgag
    14161 tgaaaggcag catatggcgt atgtcgtaac tgagaactgc atcctgtgca aatacacgga
    14221 ttgcgtggaa gtctgcccgg tggactgttt tcgcgaaggc gagaactttc tggtcatcga
    14281 tccggaggag tgcatcgact gcacgctgtg cgtggcggaa tgtccggcgg aagccatttt
    14341 tgcggaggac gaagtgccgc acgatcaaca gcattgcatc gcactcaatg ccgaactggc
    14401 gcgcagctgg aagccgatta tcgcccggcg cgacccattg ccggacgcgg atgagtggaa
    14461 cggcaaggag aacaagctcc cccacctgaa acgctgacgc agtgctcgga cagcgttatc
    14521 tgcacgccgc acgtgaagtg ctggctcagg cccggcaaca cggtttcgag ccggttcagg
    14581 ctcgtgtgct ggtgccgaat tttcacgccg gtaccgaact ggcgcgcgcg ctggtcagcg
    14641 tcagcggtca gccggcgctg ctgccgccgc gcatgtcgac atttcccgcc tgggccgcag
    14701 aagcgggtgt ttcgggacca ctcctgccgg acagtcggcg tgcgctgctg gtgtatcagg
    14761 cgttacgtgc gcggcgctgg ttcgacgaat cactgctgtg gccggtcagt atcgagctgg
    14821 ggcggctgtt cgacgaaatg acccggcagg ccatccgcct gcccgaagac gaagtggtat
    14881 tcgcccggca gctgatggct gcttaccggg tgcgggcgca tcagtatgtc gagttcgagg
    14941 cacgtctggt gcatgaactg tggtttgcgc tgatgcgccc cgacgctggt ggtgcgcgca
    15001 tgcctcaggt ccagtacgcc atgcagttgg cctggcaggc ggatcacgcg gacatgccgt
    15061 tgttcgtgct cgggctgggc gggctgactc gtcaggaaag cgaatgcctg gagcgctatg
    15121 cgctcaggca gccggtgtgg tggctcgaca tgcgcgctcg ggatgcggcc gcagcctgtc
    15181 tgcacgcggc gtggccggag acgcaggaaa ccgggttgat cgggcgtgcc ggccagctcg
    15241 tgcaacaact gaacgagagc ccctggcccg gaaggttgac gctggtgggc gcgcgcgggc
    15301 tcgaacagga agcgcgcagc gcagaggcgc agatccatcg ctggctgcat gcaggtcata
    15361 cccgcattgc tgtggtggtg catgaccgcc tgacagcgcg gcgcctgcgg gcgttgctgg
    15421 aacggaacag catcctggtg gccgatgaaa ccggctggtc gctcgatact acggtggctg
    15481 gcgggctggt catgcgttgg ctcgaagcgg tgcgcgatga ttttcccgcg caggcggtac
    15541 aggactggct gcaggcgccc cagtggctcg tgcactgtaa ccgcagcgaa cgcgagcgca
    15601 tcaacgtgca ggccagccag ctgctgcgtc gcaaaccgcg tttgtcgggt ctggagggct
    15661 ggctggcgca ttttgccgcg caggaccaac atgcgttgct gcatgccgcg ctcgaccaga
    15721 tgtttcaggc tgcgcacggg ttgcgcggca gccagcgtcc gctgaccgcc tggctgggtg
    15781 ccctgctgga cagcctggcg ttgctgggga tcgatgcatg gttgcaggct gatcaggcag
    15841 gcgcgtcact actggccctg ctggcggcgc gacgacagga actgctcacg gccggccagg
    15901 cgttgacgct ggcggaattc atccgatggg tggagcacga actgcaggcg gggagtttcg
    15961 tcgacgaaca tgtggacagc ccgctggtgt tcactcatct ggcggcgctg cgactgcgac
    16021 gctttgatgc tgcgttgctg ctgggggcgg atgatcgcca gctgccagac aacggcgcat
    16081 tgtctggttg gttcaatcag cgggtgcgca gtgagcttgg tctgccattg cgggccgacc
    16141 tgtggcgaca gaccgaggcc gatctgttct ccctgttgac cgacgtgccg cgggtgtgcg
    16201 tgatctggca ggatgagcgc gatggcgagg ccaatccact cgcatcctgg ttcgaacggc
    16261 tcgatacact gcatgtcctg gcttggggtc gtagcttgcg tagcgcgccg gtgcgctggc
    16321 aaccgcagcg gcctgctgtt gctgcgccag ccatggctcc gcgcccgatc gtgcctgcag
    16381 atgagcttcc aacgcgtatc agcgtgagcg cgcaccacag tctgatggct tgcccgtacc
    16441 agtttttcac ccgtcacatc cttgggttgc gcatgccgga ggatacggat gcaggcatgc
    16501 agaaatcgga ttatggacag cgggtgcacg agatcctgca cacattccat atgcgccacc
    16561 cccgcctgac cgaactgcag cccgaacagg ctgaggcggc cctgggtgaa atgagtcgac
    16621 ggctattcct gcgcgatctg cgtcaggatt tcatggcata tggctggctg gagcgttggc
    16681 tgcaacaggt gccaggttat gtgcgctggc agctggaacg tgaggcgcaa gggtggaatt
    16741 ggactgaggg tgaatgtgat ttcgagcggg cgttcgaaat tgatgcaaca cggcaactcg
    16801 tgttgcacgg gcggatcgac cgcatcgacc ggcgcgcgga tgacgtcgcc gtgctggact
    16861 acaagactgg ctcggtcaaa gggttgcgcg acagcgccgc gcaaccggag gaagacgtgc
    16921 agctggcatc ttatgcattg ttgctgcagc agcctgtcaa ggaatcggca tttgtggcgc
    16981 tggatggcga ggctgtggat tcagtgccgc tcaccgaacc tctcgacgaa ctggcgttgt
    17041 gttgcgaaaa ccgtctggta gacatcttcc gccgtttgca tgctggcgac ggcctgccgg
    17101 cccagggtac ggatgcggtg tgcgagcatt gcgaggcgcg cggattgtgc cggcgtgatg
    17161 accgtatcgg ggaatccgcc ggctagaacc gcactcatgc cgcggcctgc gtggcgtggc
    17221 ggcatggggg tgggtcatgg cgtacgcgcg ctgcggctca gctccggctg acgtgctgct
    17281 cgatctcgtc gatggttgtg tggcgaatgt ccttgccctt gaccatgtac accacgtatt
    17341 cggaaatatg gcgtgaatgc gcgcccatgc gttcgacaga cttggcgatg aacatgaggt
    17401 cgatgaaatt tttgatggtg cgtggatctt ccatcatgta ggtgatcagg ttgcgcagca
    17461 gcaggtggta ttcctcgtcc acctcgcgat ccttgcgtgc gatgagcacg gtatcggcga
    17521 catcgagccg ggcaaacccg tcgagcgcgc ggtgcagcat gtcgagcacc acttcattca
    17581 tgaagctgat ctggttgaag cggttgggca tcacgcggcc ggcctcatgg atttcgcgtg
    17641 tcatgcgcgc aatcttggcg gcttcatcgc caatacgttc gaggtcggtg atggtcttga
    17701 tggcggtgat gatcatgcgc aggtcggacg cggttggctg gcgtcgagcg atgatctgcg
    17761 cgcaatcctc gtcgatcgcg acttcgagcg cattgaccgt atggtcgttg gacacaaccg
    17821 tgtcggcgag ttcgaaatcg gcattaagca gcgattccat ggcctgactg atttgctgtt
    17881 cgaccagacc gcccatttgc agtacacgcg aacgtacgtt ttcgagatcg gtatcgaact
    17941 gcttgaaggt gtgttcttgt ggcatgttga tgcaactcct cggacattgg ccgattataa
    18001 ccactcaatg ccataattgt gtgacagttt gatgattggt caggcggaaa atggaatttg
    18061 caccgggacg ccatcgattt cgagtgtgag cacccagtcg tgccggccgc tgacgcacac
    18121 cggcagaatc acgcgggatt gccagtggcg gccatcgtca gacagcagac ggtagcggat
    18181 gtcacccata tccatgtcgc gcatggcaaa gcgggcgtgc actgcatgtg ccggaggtgt
    18241 gacgatgcgc agcgtgaacg gctgaagtgc ggatggcgtg ccgtcgaagc tcagttggac
    18301 atcgttgccg tcgagcgcga tgcggcaggt atgcggcaac tgtggacagc tggccagcgt
    18361 gacctggcgg tgcgcgtcgc gccacagcgc aacgcctgcc ccggccagcg ccagtacgac
    18421 cagtgcgccc ggtaccgcaa tgcgcagcca ccagcccggt ttcattgcca gagcccgcgc
    18481 aacagggcga ttccctgcgc accgtgctgt tgcgcggtag cgagcaggcc gggttccatg
    18541 ccgcccagcg catagactgg cagactgcgt ccggcgatca gcgtcgcaaa tccatgccag
    18601 cccagggtgg tggcaccggg atggctggcg gtgggcagta ccggcgatag cagggcgtag
    18661 tcgcagccga actgctcggc gcgatcgagt tcggcagcat catgacacga cgcaccgatc
    18721 aggggaaaat caggacgcg
//
LOCUS       DAIANL010000043         4781 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_448412, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000043 DAIANL010000000
VERSION     DAIANL010000043.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448412"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          954 a         1638 c         1265 g          924 t
ORIGIN      
        1 ttactcgatc accttggcca ccacgccggc gccaacggta cggccacctt cgcggatcgc
       61 aaagcgcagc ccttcttcca tcgccaccgg cgcaatcagc gcaacgttca ccgacacgtt
      121 gtcgccaggc atcaccatct ccacgccagc cgccagttca accgcaccag tcacgtccgt
      181 ggtacggaaa taaaactgcg gacggtatcc gttgaagaac ggcgtatgac gaccaccctc
      241 atccttcgac agcacataga tctccgcgct gaatcgcgtg tgcggcttga tcgatcccgg
      301 cttcgccagc acctgaccac gttccacatc ttcacgcttc gtgccgcgca gcagcacacc
      361 cacgttgtct cccgcctgac cctggtccag cagcttgcgg aacatctcca cacccgtgca
      421 cgtggtcttg gtcgtcgccc ggattcccac aatctcgact tcctcgccca ccttgatcac
      481 gccgcgttca atacgccccg tcaccaccgt gccgcgtccc gaaatcgaaa acacgtcttc
      541 caccggcatc aggaatgctc cgtccaccgc acgctccgga tccggaatgt agctgtccag
      601 cgcatccgca agccggaaaa tcgacggctc gccaatctcc gactggtcgc cttccagcgc
      661 cttcagggcc gagcccacaa tgatcggcgt gtcatcacca ggaaactcgt acttcgacag
      721 cagctcgcgg atctccatct ccaccagctc cagcagttcc gcgtcgtcca ccatgtctgc
      781 cttgttcatg tacacaatga tgtacggcac acccacctga cgcgccagca gaatgtgctc
      841 gcgcgtctgc ggcatcggac cgtccgccgc cgacaccacc agaatcgctc cgtccatctg
      901 cgccgcaccc gtgatcatgt tcttcacgta gtccgcgtgc cccggacagt ccacgtgcgc
      961 atagtgccgc ttctcggtct cgtattccac gtgcgccgtg ttgatcgtga tgccgcgcgc
     1021 cttctcttcc ggcgccgagt cgatgtcagc gtagttcttc gcctcgccac caaatttctt
     1081 cgacagaatc gtcgtgatcg ccgccgtcag cgtcgtcttg ccgtggtcaa cgtgtccaat
     1141 cgtccctacg ttcacatggg gcttggtccg ttcaaacttg ccttttgcca tgatgtccta
     1201 tcctctatag cctaacgatg actgcattca aaccgaaact ggtgcccatg acgtggattg
     1261 aacacgtggc ctctccctta ccaagggagt gctctaccac tgagctacat gggcatgcga
     1321 ccgcaaccat tacaaccggg cccgcgcctt acggaccccg cactacaaca cacagggcct
     1381 gtctggagcg ggtgaaggga atcgaaccct cgtcataagc ttggaaggct tctgctctac
     1441 cattgagcta cacccgcgaa tctgccggac catcaaccgc cgggggcgcc aaaaacgccg
     1501 ttccgcactg ctcgaaattc tggtggaggg ggaaggattc gaaccttcga aggcagagcc
     1561 gtcagattta cagtctgatc cctttgaccg ctcgggaacc cctccaaaac aagccggcta
     1621 ttatcttaca acatccgccg cgcgtcaaac attaaaacga aagtgcatga catccccgtc
     1681 ccgcaccacg tactccttgc cttcgagacg catttttccc gcatcctttg aacccgcctc
     1741 gccaccgcag gcgatgaagt cctcataacc gatcacttcg gcgcgaatga acccgcgctc
     1801 gaaatcggtg tggatcacac cggcggcctg cggcgcagtg ctgccgcgca cgatggtcca
     1861 ggcgcgcact tccttgaccc cggccgtaaa ataggtttcc agacccagca gcccgtaagc
     1921 cgcacggatc agacggttga gcccgggctc atcgaccccg agatcggcca ggaacagcgc
     1981 cttgtcctca gctgccatgt cgaccatctc cgcctcgagt gccgcacaca ccaccacaac
     2041 cggggcaccc tcgttcccgg caaatcgtcg taccgcatcg agcagcggat tgttatcaaa
     2101 tccattctcg tccacgttgg ccgcatagag taccggcttg gcggtgagca ggcacaacgg
     2161 acgcaccagc acccagtcag actcgtccag cgtcatggca tgcactggct tgccagtatc
     2221 gagccaggcg cgcaaccgtt cgatcagttc cacggcgcgc agcgcttcct tgtcgccagc
     2281 gcgtgcggtc ttctggtagc gcaccacggc cttttccagc gtcgcaaggt cagccagcgc
     2341 gagttcggtc tgaatggtct cgatatccgc caccggatcg accttgccgg caacgtgcac
     2401 cacgttgtcg tcctcgaagc agcgcaccac atgcatgatg gcgtcggttt cacgaatgtt
     2461 ggccaggaac tggttgccca gcccctcgcc tttcgacgcg ccagccacca gaccggcaat
     2521 gtccacgaat tccacaatgg ccggaagcac gcgttcggga tgaacgatgc gcgccagcgc
     2581 atccagacgc gcatccggca cctcgacaac cccggtgttg ggttcgatgg tgcagaatgg
     2641 gtagttttcg gctgcgatgc ctgcacgggt caacgcattg aacagcgtgg acttgcccac
     2701 attgggtaaa ccgacgatac cgcatttgag actcatgaat accccttggc aaacctttgg
     2761 cacagcgtgc atgatgcgcc cgcatccggg cgcccgtcag acttcgctgc cgtgcaattc
     2821 tcgcatggcg gcagaaaaat cgccgcgcag caggttgggc aactgaagca gactgcgctc
     2881 cagcgccgac tcgatgcgct cggcatcgtc ggcacgcggc acattgagca cccacgccgc
     2941 taccgcatcg cggtgcccgg gatgtccgat ccccagacgc aaccgccaga actgcgggct
     3001 gccgagcgtg gctacgatat ccttcaatcc gttgtgtccg ccatgcccgc cgccaaactt
     3061 gaagcgcgcc acgccgggca gcaggtcaag ttcgtcatgc acgacgagaa tttcttctgg
     3121 ctcaaagcgg tagaagcgaa ccagcgaccc cactgacttg ccacacgcgt tcatgaatgt
     3181 ttgcggcaac agcacatgca ccggcgcatt gccctggcta cgcccagtca ggccgaaaaa
     3241 ccggctttct gcctgcaacc cgatgcgcaa ttcacttgcg cagcgctccg cccaccagaa
     3301 tccggcattg tgcctggttc tggcgtattc gcggccaggg ttgcccagac cgacgatcag
     3361 gcgaatcgac atgaccctgc ctccggatac gggtggatca acatcggctc atcggcggtg
     3421 cacacgggca acggtttcag cgtcgcacca cagggcagat caccagacgc cgggcctcgt
     3481 cgcacacctg gtccgcgttt cgcactgcgg atgaaccgga accgttccta gcctgccgct
     3541 tcttcggcct gcgcgccgcg cggcagcaca atggtggcca cgaccgggtt ctcgcccttg
     3601 agggtagcca ccgactcgac gccggcaggc agcttgatgt cagccacatg caggctgtga
     3661 cccgccgcca tctgacccag gtcgacttcg atgaactcag gcagactgcc gggcaggcaa
     3721 ctgatttcaa gttcggtcat gacgtggctc acattgccgc ctgacagttt cacacccggc
     3781 gcaatgtcgg cattgacaaa gtgcagaggt acgctgatat gcagtttatg ggtgctgtcc
     3841 acgcgctgga agtcgatatg ctgcacttcg ctgcggaacg gatgcatgac gaaatcgcgc
     3901 agcagcacgg attccttggc accatcgagc accagcgtca gcaccgaggc gtgaaacgct
     3961 tcacgcttga gcgcatgaaa cagcatgttg tgatccagtt cgatgctgac ggccggcttg
     4021 ttgccaccat acagcacgcc aggcgtcttg ccactgcgac gcaggcggcg gctcgcaccc
     4081 ttaccctgca cgtcacgttt ttgcgcgata acttccatgt ttgctccagt ctgtgattca
     4141 ggccttgcga cctttgatat acattctgcc cccgcgacca ggcggcagac gttgattgca
     4201 ggcatcctgc ccgcaaattc ctattccatg aacaacgaac tcaccgaatc ctcgttgctg
     4261 atgcggcgga tggtttcagc cagcagaccc gccacagaaa gctggcggat gcgcggactg
     4321 gccttggcat cctcgcgcaa cggaatggta tcggtcacca ccagctcgtc cagcgcggac
     4381 tggttgatcc gctgcactgc agtgccggac agcaccgggt gggtgcagta ggccagcacg
     4441 cgtcgcgcac cacggtcctt gagcgcctgc gcggcttcgc acagcgtgtt ggccgtatcc
     4501 accatatcat ccatgatgat gcaggtgcgg ttgtccacat cgccgatgat attcattacc
     4561 ttggccacat tgggccgcgg ccgacgtttg tctataatcg ccaggtccga ttcgagccgc
     4621 ttggccagcg cacgcgcccg caccacacca ccaatatccg gcgagaccac aaccagcttg
     4681 tcatacgact gcttccacac gtctcccagc aggatcggcg aagcgtagat attatcgacc
     4741 gggatgtcga agaacccctg gatctggtcg gcatgcagat c
//
LOCUS       DAIANL010000044         7356 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1675277, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000044 DAIANL010000000
VERSION     DAIANL010000044.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675277"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1356 a         2136 c         2341 g         1523 t
ORIGIN      
        1 tgcaccgctg catgcgctgg ctgatgcttg cgtcacgcaa ctcgatcacc atcgcctgcc
       61 ggaagactca caacgtcaag cagaacgatc ttctgggtta acaccaaaag ctcaagcgca
      121 cctggcacgg tggggttatc cgtttgtact tgatcaatgg caatttcaca tgacgctgtg
      181 caacagcggc gttaccaacc caacattact gactcatgcc aaacgccact tcgaagaagc
      241 actaaaccaa cccagacaag tcatttcaat cgccacgttt atcgaagaca gcccaggcgc
      301 accattgcgg ctaagcaaac gattccagtt aggacaagtt gctggccatt ttgcaaaccg
      361 tcaacctgag gttatccgtt aaagacgcgg tgattaaaga aaaccgtcgc acacatacaa
      421 ccaacaacaa tgcggtgggt gcctccgaac ctattgataa tccaacgcca tggtaacgct
      481 gatcaccaac acgacaataa gtagaatata caaacagttt tttgaccatg cgctgtcatc
      541 agtcgtagta aagtcggcca gcgccaaata caactcagta tccgctcaaa gcaagcgcaa
      601 ccagtaaaaa atgtccccgt ataacccgcc caactcagcc agctgatgcc atggcataac
      661 agcgcgaaac tcagtcgggg ttgacttcgc cagtccagtc gcaatacaac ccgctgatat
      721 tgagtgcgcg ttccatgctg acgagcatgt cttgcaggtc ggcgaattcg tgagcattgc
      781 ctgcgctggc atgacgcacg atccagcagt atcttgcatc ggtacgcgca gcgctcgaaa
      841 tgaacgattg taccgagcgt acgccgtcat gcgcggcgag ccactctcgt cccttgcgca
      901 gtagcgcacc ggtttccgtc tccgggatcg gggttgtcgg gcgcaggcag atgcgcagtt
      961 cggcttccct ccacgcgcgg cattgctgta gcacttcggc agcgcgcccg ccactgcccc
     1021 acaggcggat catgtgcgcc acccggtcac gaatcgcttg tccactgtgt tccagccact
     1081 gtgccgggtc ggcgtcgcca tgttgccgga ccgcttcgcg gatggcacgg gcgccggttt
     1141 cggccagcgc cgaataataa ttgatctttg ccacgccgtg ggcgatgagc ttgcggtact
     1201 gatcgtcgct gagtccggtg ccgccgtgaa tgaccagagg caggccaacg gcttcactga
     1261 ccttggccag gcgggtcatg tcgagctttg gcgggtgctt tggcatgccg tgcacggtac
     1321 cgatggatac ggcaaggcag tcgatgccgg tgcgctcggc gaaggccttg gcttcggcgg
     1381 gtgatgtgta gctgatttcg tccggaacgc tctcgtggtt gctgtcgttg atccagggaa
     1441 tatggccgat ttcgccttcc acggcgaggc cgaagcggtg tgcgagctgc actgcggcac
     1501 gggtcaggcg cagattgtca tcgagcgcaa gcgtagacgt atcgatcatg acaccattgc
     1561 agccattgcg gatggccatg tgcaccgcgt tcatgtcgtg accgtgatcc aggcacagcg
     1621 ctaccgggat cgaggcgcgt tgcgcggctg aaagcgcggt gctgaccagc atgagctgcg
     1681 ggtcgctgcg gaatgcctct tcgcgaaaat tgaggatcac cggcgcgcga caatcttcgg
     1741 ccgcctggag aacgccgcgc aacatttcga aatgcgaaat gcgaaatgct ccgatggcat
     1801 atgcgtggcg gtgcgcatgg tccagcatgt cagtcaggtg gaggatgggc atgatgtcgg
     1861 gccggaaagg tggtcgatgt cggacggaga tggcgtgaag tctgcattgc gtaccggcat
     1921 ggcgaatcgg ccggcaggca acgcctgcca acctgtagcg cggcagtcac gccagtgatt
     1981 gcattcgcct gcagcgtgcc tgtctgcctg ccggggcaat ccgggcctag tcgtcgtcac
     2041 ccagttccgg atcccagccg gcgcgccggg ctgtctcgat cgactgcgca tacatgtgcg
     2101 cgtgctgctg tcgcaggccg tctggcaggt tgcttggcat gtgaccgtaa ggtgcccagg
     2161 cagcgaacac gcgttcgtac agttgctgta tgcgcccggg ttcccaaccg gcgcagcttt
     2221 cgtctgcagc gaccagtccg aacaggcgcg catcgagcac gatacgcgcg agcggggtca
     2281 gtccattggc aaggtcttgt tcgagtccga cgtaattcgt atcgttcatt ttgtcagtcc
     2341 tgcataaagg gtgggcagac gttcgggcaa gcgctcgacc cgatcgagaa tagtatagtt
     2401 gcgcgcgccg aaaatgcgtg aaacatactg gtcggcgcgg gggtccaggc tcaggcagaa
     2461 ggtgacgatg ccgttgcgag ccagttgttc cacagctttc ttcgtgtcgt tgcgcaggta
     2521 ttgcggatcg cgcacgtcga tgtccgccgg ctcgccatcg gtgagcacca gcaacatctt
     2581 cttggatgaa ttgcgccgtt gcagatcgcg tccggcgtga cggatggcgg cacccatgcg
     2641 tgtcgacagc tgccctgaca tgccggcaag ccgcccgcgt gcggtatcgt cgtatggctg
     2701 gtcgaaatcc ttgaagcggt agtagtgcac gtcgtggcga ccatcggagc agaagccgtg
     2761 gatggcaaac gggtcaccta ccttgtggat cgactcggcc agcagcacgc aggcttcgcg
     2821 tgtgaggcca agcacgctgt gctcctgtcc gggcaaaatg tcgttggtcg attccgacag
     2881 gtcgagcagc aacagcaccg aaatatcgcg ggtcttgcgg atcgaacgca tcatgatgcg
     2941 tggatccggc tggcggcgct gacgcagcga caccatcgaa tcaattgccg cattcaggtc
     3001 gatttcgtca ccgtcttcca gtttgcgctg gcgtaccacg ccctgcggtt gcagcgcgtc
     3061 tagcaggtgg cgcaaccggt tcagcgtaag ccggtggttg aaggtaatgt cttcgatcag
     3121 ttgcagttcg ccggcgtgag cgcgtttttc gatgacgctg gcccaggacg gtcgttcgag
     3181 ctggatcagg taatcccatt ccgggtaatg cgtcggtggg gcaacgggtt ctttgccttc
     3241 gagctggttg tagctgacgc cgttgtcttc ataggggaaa agttcgctgg cgagcaccca
     3301 gatttcctgc gcgtcatcgc ctgcgagttc gcagtcgatc tcgttggcca tttccatgac
     3361 gctgacgtat ttgcggacct gctgaatccg ttcgtagccg gcctgctgtg cccggtcgaa
     3421 atcgaactcg gcaaattccc agaaatagcg gttgtcatcc cggtagggtg cgctctgcac
     3481 gtcggtacgc gggtgaaacg gaatgcgcaa ctgcgccagt tcgtgggcga gctgaacgcc
     3541 gatatcccat gaaatctggt ggtcttcgag ccggtcctgc actgcagcaa actgtgcgcg
     3601 cgcgctggcg acgagcggat gatcgtcctg acaggccgga tcgagcagcg cgcgtgcgat
     3661 gcggttgagc cagtcaccga tggatgtcgc ctgcgctggc gttgcgacgt gcagcgatgc
     3721 ccacagcgtg gccaggccgg gaaaactgcg aatggccagc tgttcgacac gcgcgtcctc
     3781 gatgacaccg atgaccgcca tttgcaacgg gttcagtccc tcggccgaaa tcggtgcgcg
     3841 ggtgtacatg atgtgcgcgg cgcaatgtgc cgtgacggca cgatagagtt ccaggccgct
     3901 gaccgggccg atgtcgtcat aggcatcggg gatgtgcagc aggtaattct cgatataggg
     3961 gcgctcccct tcgcggcgtt cgtggtcgcc ggaggttggg cgcatgaaga aatcgcggcc
     4021 ccacagtgcg cgcagataca tgttgatgcg gcgctggatg tcgacgaaca gggtgccctt
     4081 gcgctcctgt tgcagcatcg attgggattc cgggctttcc aggctgaaat aacgcacctg
     4141 ttccgggtag tcggtgcggt gcgcctgcgc gccccacatg gcccagcgac gcaacccgcc
     4201 cagcgtgagc ttgcccagca gcagttcgag gcggtcgagc atcggcctca cgccgcgcgg
     4261 cgcctgcgag atcagctggt cgagcaggtg caggtagccg cggaacagct cggcgtcacc
     4321 gaggcggctg gcagccagcg gcgcggtgga gaacagcagt gcgatgactg cgccactggt
     4381 gcgcgaggtc atcatcatgg cgctctggat caggtcgggc agatcgtcct cgccgatttc
     4441 gcgcgcgatg agcggagcgg attcgatgaa ggcatgaacc agctcctcgc cgcgtcccag
     4501 atgcagcagc gcgcgcacgc cctgcaggta gtgctgcaac ccggcaggac tcagcgtgcg
     4561 cgttgcctcc gtccagttgg cgtgcaggat ttctctggac gcttccggaa gctcgtccag
     4621 cagatcttgg tggtcttcga gcatgatatg catggtattc agcctggtgg ttgcgtgaca
     4681 ggcgtgccgt gtgctggccg accgttcaga agaacgtggt tactgcagcg tccagcgcat
     4741 cgcgcatgtc ggggtcgtcg gtgatcgggc gcaccagcgc cacgcggcag gcagcctggg
     4801 ctgcgacacc actggcgatc agcgaggccg catggatcag catgcgcgtc gagatgcctt
     4861 cgtccagccc gtgccccttg aggttgcgtg cccgttcggc gatctgcacc agcttttccg
     4921 ccacatcggt ggtgaccccg gtttcatgca ccaccacttc ggtttcgatg gcgcgggacg
     4981 gatagctgaa gtcgagtgcc ccgaaacgtt gcttggtgga ttgcttcaga tctttcatca
     5041 agctctgata gccttggttg taagaaatca ccagctggaa atcgggatgg gcggcaatct
     5101 gttcgccttt cttttccagc ggcagcacgc gacgcgtgtc ggtcagcggg tggatgacca
     5161 cggtggtgtc ctggcgtgct tcaaccactt cgtcgagata gcagatggcg ccatggcggg
     5221 cggcgatggc cagcggccca tcctgccagc gtgtgccgga ggcatcgagc agaaaacggc
     5281 cgaccagatc ggaggcggtc atgtcttcgt tgcaggcgac ggtgatgagc ggtttgccaa
     5341 gcttccaggc catgtattcg acaaagcgcg tcttgccgca gccggtcggg cctttgagca
     5401 tcatgggcat gcgcaccgag tacgcggctt caaacagttc cacctcgtct gcgacagctc
     5461 ggtagtacgg cggggtggta atccggtaat cctgggtcaa gtcattcatg tgcggattcc
     5521 tgcaaaacgg ttggtgcggg cagcacgcag gatgcgtcct gcccgcgggg ttgccaatgg
     5581 tggatttcgt cgggatcaga ccgccttgcc gcgatagatc accatcgaag ccccctggct
     5641 ctggttaaag ttgttgtagc cgatcaggcg cacgtggttg cccgggttgg ccttgtggca
     5701 ggcttcggct tccttcagga tggcatcgac atcggtttcg ccgaacatcg gcagcttcca
     5761 catgtaccag tacgaatcca tcaggtgctc cggttcggtg tgctcgatgg ccggattcca
     5821 gcccttggag acgacgtact cgacctgttt gcgaatctcg tcctggctca tggccggcag
     5881 ataggaaaag gtttcgtatt tccggctgtt cggatcgctg agacgggagg ggtaatcctg
     5941 catggtgctc atgggtattt cctttccttg ttaacggttg ggcgttggcc gtgcccggca
     6001 tgcgcactgg catgccggga catcggttta acggtgggta acgtccagtt tgtcgacggt
     6061 gtcgaactcg aacttgattt ccttccaggt ttccattgcc accttgagtt ccgggctgtg
     6121 ctgggcggcg ttggtcagga tcgccttgcc ttccttctcg atggcgatgc ccttgttgcg
     6181 cgcttccacg caggcttcga gcgccacgcg gttggccgcg gcgccggcag cgttgcccca
     6241 ggggtggccg agcgtaccgc caccgaattg cagcaccgag tcgtcgccga agatgctgac
     6301 cagggccggc atgtgccaga cgtggatacc gccggaggcg actggcagta cgccgggcat
     6361 cgatccccag tcctgatcga agaagatgcc gcgcgaacgg tcttcgcgaa tgaactcgtc
     6421 acgcatgata tcgatccagc ccagcgtggc ttcgcggtcg ccttcgagct tgccgaccac
     6481 agtacctgag tgcaggtggt cgccgccgga caggcgcagc gccttggcca gcacgcggaa
     6541 atggatgccg tggtgcgggt tgcggtcgat caccgcgtgc atggcgcggt ggatgtgcag
     6601 caacagaccg ttgtcacggc accagttggc cagaccggtg ttggcacaga agccgccagt
     6661 caggtagtcg tgcatgatga tcggggcgcc gatttcacgc gcatattccg cgcgcttgta
     6721 catttcttcc ggggtcggtg cggtcacgtt caggtagtgg cccttgcgtt cgccggtttc
     6781 gcgctcggac ttctcgatgg cttccatgac gaaatcgaaa cgttgcttcc agcgcatgaa
     6841 tggctggctg ttgacgtttt cgtcgtcctt ggtgaagtcc aggccgccac gcaggcactc
     6901 gtacacggca cgtccatagt tcttggccga caggcccagc ttcggcttga tggtgcagcc
     6961 cagcaacgga cgaccatact tgttcatgat gtcgcgttcg acctggatgc cgtggggagg
     7021 cccgccgcag gtcttgacgt aggcgatcgg gaaacgcacg tcttcgagac gcagcgcgcg
     7081 cagtgccttg aagccgaaca cgttgcccac cagcgaggtg aacacgttga cgaccgagcc
     7141 ttcttcgaac agatcgatcg ggtaggcgac aaaggcgtag aagcaggtgt catcgcccgg
     7201 cacgtcttcg atacgatagg cgcggccttt gtagtagtca aggtcggtga gcagatcggt
     7261 ccataccgtg gtccaggttc cggtcgacga ttcggcggca acggctgcgg ctacttcctc
     7321 gcgcgccacg ccgggttgcg gcgtgatctt gaaaca
//
LOCUS       DAIANL010000045        41224 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1068264, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000045 DAIANL010000000
VERSION     DAIANL010000045.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 41224)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41224)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..41224
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068264"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         8087 a        13357 c        12202 g         7578 t
ORIGIN      
        1 gcgaaggtcg ggagttcgat cctcctcgtc tccaccaata tttaacgccc aacccctctc
       61 ggttgggcgt tttcatttct gcttgccgca cgtcgcgcgg ggtttcggcc cattttgcct
      121 gtgccgaccg tcggcggaca aggggccacg acgcgcccgg ttcgactctc ttccgcccta
      181 ttgtgagcgt cgcccgtcat ctgaaccatc ctgatcggcg gcaagtccat ccggggcgcg
      241 tggctgccgc gcggcatggc gatccagacc tggcgcgcag cagtcccttc tggcgggcca
      301 gatcgaggac gcgctggctg tgtgtgtcgg cgggcctggc cgtctcgtgt tccatatatt
      361 cgcccaatgt agcattgtgt agaaaaaatg aaacatgccg aggcgacgac cgggactgcc
      421 cgatgccgcg attagtcgac acattcgccg caccgtacgc ggagaatgac ttgccgttgc
      481 ggcgaattgg gtgtttaatc tccgcatgga tagcggagaa caaccctaca tctggcaggc
      541 tggcgactgg cccaactggc gctacgacct gtccgcgctg acgtggccgc tgaccgaggt
      601 cagccgcgcc caaggcctgc tgctaggccg cttggccgac gtggggctgg cgctgcgcga
      661 tcaggccagc cttgccgcct tgaccgagga cgtggtcaag accagcgaaa tcgaagggga
      721 agtgctgaac gtcgaatccg tgcgctcatc catcgcccgg cgtctgggcg tggacatcgg
      781 cgcggtcgcc ccagtggatc ggcacgtcga aggcatcgtg gagatggtgt tcgacgccac
      841 gtcccatgcc gctgccccgc taaccgtcga acgcctgttc ggctggcatg cggccttgtt
      901 cccgaccggc tactcgggca tgaacaagat cgatgtgggc cagtggcgcg acgatgccgg
      961 cgggccgatg caggtcatct ccggcccgat ggggcggcgc aaggttcact tccaggcgcc
     1021 gcccgccgag gtgttggccg tcgagacgac gcgctttctg gcgtgggcga atgcggaaac
     1081 cggcgagcct gcgctcgtca aggccggcct ggcgcacctg tggttcgtga cgctgcaccc
     1141 cttcgacgat ggcaacgggc gcatcgcccg cgccgtgggc gacctgttcc tcgcccgcgc
     1201 cgatggcagc ccgcaacgct tctacagcct gtcggcgcag atccagcgcg agcgccagga
     1261 ctactacgac gtactggagc gcacgcagaa gggcacgctc gatgtcaccg gctggctgtc
     1321 atggttcctc ggtacgctcg gacgcgccgt caccagcgcc cagaccacgc tggacgccgt
     1381 gctggtcaag gcgcgcttct ggcaacgctg ggcgggcacg ccgatgaacg agcggcaggc
     1441 gaagctgctc aaccgcctgc tcgacggttt cgatggcaag ctcaccagca gcaagtgggc
     1501 caccatcgcc aagtgctcac ccgacaccgc gctgcgtgac attacgcagt tgctggaact
     1561 gggcgtgttg aggaagtcac cgggcggtgg gcgcagcacc ggctacgagt tggcggattg
     1621 gtagccggag ccagatacgg caatcaaact ggcagcgatg ccaaggcccg ctgcaacgat
     1681 ctgtaatggt cgcccgaggc ggcctgcacg tcgatgccca gattcttggc gttctgcgac
     1741 cagcgctgcg cccaggcgaa gacctcgtcg cgatcaaaac ggatgagctt ctggacccgg
     1801 cgatacggaa tgcccaatgc gtctcgcgtc tgcttttgcg tgaagatgta caggggcagc
     1861 ccggtcacat acaaaatctc gcgtgggtcc atgtacctcg agccattcgg gaggacgacg
     1921 tcggtggcgc tctcccaggt cgatgcccgg agggcttggg actcgtaatc gaggacttcg
     1981 tcaatcgggt acacgacgcg cctggacatc ttcatgaagc gcggtccgct ccctcgctgc
     2041 gccagcgctg tagtgtcttg gggctgatat tccagcgcga ggccagttcg ttttcgttca
     2101 tcatcatctt ctgcatggtt ggctccgatc gtggttgagg gcaacgcttt caggcgtagc
     2161 tccggccaca aagccaagtc ctcggcaggg catctatgcg gtttcagcgc aatgcgagca
     2221 tcagcgggtc atgccaatga atcctgatca gcctgctgaa acccattgct atggggacgt
     2281 ctcggcatcg tggaagatac ccggttcaag gttccggtaa aatccgccac caatattcaa
     2341 ggccgaccgt tctctatcgg cctcttcaat ttccgacttt cccccaagcg ctgccgggaa
     2401 ccgctcagcg tcgtactccg ggcttgattt catccactcc ggcaggcgct agagtgcatc
     2461 ggccggatcc ttcaattgcc catgaacagg gttaattacc aacggttcca gcgcgaacgc
     2521 ctgcagggcg cagccgctcc caaagcgatg gcgacgtgcg cacatgcacg tacaccatgc
     2581 agcgcagcgg cgtcacattc tgcctccgta cgttcgcgac gttcaatcca tcccacgatg
     2641 attttactgg gcgagcactc cgtccagacc tggctggaac atccatctga atcgagacgc
     2701 gattgttgat tcccatggaa atagtgttgt actattattt ccatggaaaa ggaatctggt
     2761 catgaccgca tctgcccgca aacttgctgc aaatccggat tcatcttcgg tactgtccaa
     2821 agctgtcgca cgggccgccg agcggctgga tatttcgcgc accttgctcg ccaaaatcct
     2881 gggggtcagt ccgcccaccg tgacccgcct ctacaacggt gagtacaagc tggacgaacg
     2941 acgcaaggaa tgggagttcg ccctgctgtt tgtgcgtgtg ttccgatccc tcgattccat
     3001 cgtcggcaat gagcagacgg cacgtcagtg gctcaacagc gaaaaccggg ggctcaatgg
     3061 ccgcccggtg gatctgattc gacaaacaga agggttggtc cgtgtcgttc actacctgga
     3121 cgcctgccgc ggtctcgtct gaggcctttg cctggcatgg ccaggtctgg cgcatggtgg
     3181 aatcgcagca cattgccgcc accatgaagc tggtggacag ccgcgacgag caggatctgc
     3241 tcgagtcctt gctggagtcg ggcaagccgg cccagccgga tgacacggct ggcctggatt
     3301 atttgctggc cacgccattt cgctatgacc ccaggcgggg cggttcgcgt tttcgggcag
     3361 tgatcgatcc gggggtgttt tatggtgcgg aatcggtccg cacggctggc gccgagctgg
     3421 gatactggcg ctggaagttt ctcgaagacg ccgtcgatct cgaacgtatc gagccggtgg
     3481 cacacacagc gttccgggtg gaggtcgcaa cgccggtggt cgatttgcaa cggccaccat
     3541 ttgataccga tgccgccatc tggcaacacc ccacggatta tgctccgacg cagcagttcg
     3601 ctcgcgtcgc acgcgaggcc aggactgggg gcatcctgta ccggtcggtg cgggacccga
     3661 tgccctcgtg gtgcctggcg ctgctgacgc caacgggttt tgccgagccg aaaccacacc
     3721 ctgaccggca gatttggtat ctggcggtgt cgtcgcatga agtcacgctg cggcgggata
     3781 cggaatcgat acagttttct gcggcggaat ggtagagcgc gattgctgcc ctgcaacgtc
     3841 ttgcctgcac gctcttttca gatcatcgaa tggcgtgccg gaccggtcgc cattcgcagt
     3901 gacaatggac cggaatgcat cagcggcgtg ttgcaggact cgggacacga tgattgattc
     3961 tccacggatc acgtcgatca ccacatcacc ggacaaaccg gcatgtcatc cgcctggggt
     4021 acacccagct gggaaacccg cagcacaatg cctgccggac agcgcctcat gaagggttgt
     4081 cccaatgtct gtggcaggat ctggccaaag tcctgccatt tccaccctgc tatttccaca
     4141 cagtggatgt atgattacaa ccatgatcgc ccacacatgg ccttgggcgg cattgcaccg
     4201 aaacagcggc cggccatggc cgcatgacgc tacttccggc cccagctaaa aatgggggaa
     4261 ttaccatcct gacccagtcc ttatccctat gagaccggca cagcttatcg ccatcatgga
     4321 acgcgaattc ctcagcacgg aggagggcca tcacacgcca ctcatgctgt ggggcccgcc
     4381 tggggtgggc aagtcgcaaa tcgttgccca gaccgcggcg cgccaccatg caaccatgat
     4441 cgacatccgt ctgtcgcaga tggaaccttc ggacttgcgc ggcatcccgt tccgcaccgg
     4501 cgaaacagtc gaatgggccg tgccgtccat gctgccggat gtccgacgcc acggggcaaa
     4561 aggcatcctg tttctcgacg aaatcaacgc ggcgccccct tcggtatcgg ctgcggccta
     4621 acagctgatt ctcgatcgcc ggctgggcaa ttacgaagtg ccgcacggct gggcaatctt
     4681 tgccgcaggc aaccgccagg gcgaccgggg cgtcacctat accatgcctg cccccctggc
     4741 caaccgcttt gcgcatttcg acatcgaggc caatctggaa gactgggtgc actgggccta
     4801 tcagaaccgg atcgacgacc gcctgatcgg gtttctgcgc tttcgtcccg aggtgctgtt
     4861 cgaattcgat ccggcacgca atccgtctgc tttccccacg ccccgttcgt gggaattcac
     4921 gcaccgcgcc ctgcaaaaat tcggtgacgc gcccgacctg ctcacgcccg ccctgtgcgc
     4981 ctgcgttggc caggcggccg gtatcgagct ggcggctttt ctcgccaatc tcgcgcgcat
     5041 gccggatctg gatgccattg tcgagggcca tccggtggac gcaccacaag acatcaacct
     5101 gcaatacgca gtcgcttccg cgctggcttc ccgcgcaatc cgcgccggca gccaccccga
     5161 cgcgcagaga caactgggca acatcctcga ctttgccggc aggttccctg tgcgcgaaat
     5221 gggcgtgatg ctggtggccg acatgcatcg caacattggt cagcaattgt tcgcgctgcc
     5281 ccagttcgcg cactgggcca gtctggtcgc ggacatcgtg ctgtacgggt gagccatggc
     5341 tgtccgggat actgacgctg ccacccggct tgctgcggcc cgcacccggc tgatccttga
     5401 caagccgttt ctcggggcgc tggtgctgcg cctgcccatg caggaagcac gcggctggtg
     5461 caagaccaca gccaccgaca tgcgcacctt ctattacaac ccggactaca tcgacacgct
     5521 gtcgcaatcg caaaccgaat tcgttctggc gcatgaagcc ttgcactgcg cactgggcca
     5581 tttcactcgc cgcggtcacc gcgtgcagcg caaatgggat ctggcgtgtg actttgccat
     5641 caatccgcta ctgcttcaag atggcctgac gcctcccccc gaagcagtca ttcttgccca
     5701 gttcgacaac atgacggcgg aagaaatcta cccctgcctg tccgacgaga tcgaccagga
     5761 cacgctggac caacatctgt acgacaatca gagcgaaggt ggacaaggcg gagattcgaa
     5821 cggctcacca gcggatgata cgccatcgca ggcacctcct gaccacggcg aagatgcgca
     5881 agcggaaaac cacggcggcg gccagacaca acccggtgcc ggttccaccc aatcaaacgc
     5941 agcacccgaa ctcgaacagc caccccggcc actcgacgca caggcacgct cagcgcttga
     6001 acacacgtgg cagcagcacg ccgtcgcagc tgcgcaacag gctgccgccg ccggcaagct
     6061 gagcgcagcc atggcacgcc tggtcgatac ctggttgcag ccccgcttgc cgtggcgcat
     6121 gctgctggca cattactgct ttgaatcggc ccggaccgat tacaacatcc tgcggccatc
     6181 gcgtcgcgag ggcgacctga tcctgccaag cctgaaaagc ccgcacagcg acatggtgat
     6241 tgcgctcgat acatccggtt caatcagcga tggcgagatt gacgaattca ttgccgaaat
     6301 cagtgccatc aagggcacgc ttccggtacg catcatgctg ttcgcctgcg acgcctgtct
     6361 ggccgaaggc agcccctggg tcttcgaacc ctgggacgcg ttcacccgtc cacaacacgt
     6421 caatggcggc ggtggcacgc gtttctggcc ggtattcgac tggatcgacc agcaggggct
     6481 ccgacccgat gtgctggtgt actttactga tgcgattggc gattttcccc ggcaggcgcc
     6541 cgattacccg gtgttgtggc tggtcaaggg caaacagccc gtgccctggg gaagacggat
     6601 ccagcttaac tgagcgctga gcgaatactg catggatagc gaaccccagg acctgtcttc
     6661 cgaagaccag ttgcgcctca acatcctgat gcgggccagc caggttcagg ccgtgcgcat
     6721 cgacgaagga cggatgacac ttcatgcgct caccgacaca ggtgaagcgc gggtgtcctt
     6781 gcatgccaac tgccgagcgg accggtacct gcatcgggtg aaggaatttc ttgccggcca
     6841 tgtgctgggt tcgccgggcg gttatccggt ctacctgcaa cgctggacac gcatggggca
     6901 aacttccgac aagcacctcg atgtcctgct gctgctcgga gaaccggaag ccgtggtagc
     6961 ggtggcgcat gcgccaggtc tgaccgatga gttggcacga cgtgcctggt ggtgccagcc
     7021 gaccatggaa atggcgcgct ggatgctgga aaaagacagc gtcatccagg gcaggatggg
     7081 cccggtgctg gccgatttcc tcatcgagca cctgccattc gaagccagca ccgatgcgcg
     7141 tatgcacacc gtccggctgg tgctgcacgg caagctgacc aacacggcca ccaccgccag
     7201 attatggcgc ctggcgaaaa ccaccccgga ctacttcatc ggcttcctcg aattcatgtc
     7261 cgagcaactg ccacaggacc agtccgaacg tggagatttc gcacatgccg caaccgtgct
     7321 caccccgctg atcacttcgg gcaacgccct ggccgaacgc ctgctttcac tgctcagcgc
     7381 caatggacag acctggctgc gcgccgcatc ggaagcgctg gcaaaaccat ccacctcgct
     7441 ggccgtctat gccttgctcg atgccatccg ccagtatttc gtgtccacgg gctacccgct
     7501 tgaggcagca acgctggagg acgtatttct tcgggcagaa tccatggtaa agaaccctgc
     7561 cggacagccc gaggcactga cggcactgct cgctgccgca ccgcaatttg cacgcagcat
     7621 tcaggccatg ctggtcctgt ccgcacttgc cagcagcctc gccgacccgt ggctgttacg
     7681 caacacggcc gtaggcgcgc tgattcgacg taaaatagcg cccatgaccc aacccatcca
     7741 ggacgagatg gccgcactca gaaccgccgg acattgatga cagacccacg cctgtgcacg
     7801 catgcctggc tcacacaagg aacgaatcca tgccctattt catctgcaaa ctccagtcct
     7861 tcaaccgggt cgaaccgctc gaacagttcg accagttcaa ggacgcttcc agccgcgcca
     7921 aagcattccg caaagacatg cctgccgacg ccgattacac gatcaaggtc atttttgccg
     7981 aaaccgcgct ggccgtggag gacgttctca atgcaccaag ggcgccgggc atcaccaccg
     8041 gcgaggacta ctgaaccgct tccgaccagg gccctcccat gtcaggtgaa tacaacgaag
     8101 cagcctatcg cgatgcccgt accagcatca acccgagtcc ctgcgtgttt gaaaaagcct
     8161 tgctggccac ctgtgttgcg tgcagccggg ccgggaaaca tctgctggca gaacgcgaaa
     8221 ccatcaattg tcgcgatgcc gatgcccggc aacgttgcgc cgcattgcgc gcggctctgc
     8281 gcgcgcatgc cagctttgcg ctcggagtca gcaacccgta cagtccggtt ccgcacagca
     8341 gggaaataaa agcccagtgc ggcggcctga aggggttgca gcacgccttg agcggacggg
     8401 atgccgtcac cgacgtgcat gcgttggtaa ctgtagcact gagtacattc agcgatctgg
     8461 acgcgctgcc ctatgccgcc attgtcagat cgattgccca attcaacatc aggaaacgcg
     8521 actgatcgcc ggtctggcag gcggtatgcg caatccgggc caagcatcag cacccggccg
     8581 gtttcccccg atcattggcg cggcttccat gtcctgcatc gcggcaaccc tgtccgctcg
     8641 cgcattgcca ataccatttg gctcaacgtc aatgagacga taccatagtg ggtaataatc
     8701 atcgcaacgc gcaagacact gacgggaaaa tgccatgatg aaattactcg aatcgctttt
     8761 cgaaggcgca ttatggcgca gccgcttcgt ggtaatcttt gccgtagtgg ccagcatggt
     8821 cgccggcttt gccgtgttct acatgtccac cgtggacgta gtcttcatga tcgagcatat
     8881 cgtgcactat gccgacccgg acctgagtcc ggcgatgcga cagacgttgc acgaagacac
     8941 cattacccac atcgtggaag tggttgacgg ttatctgctt gcgaccgtga tgctgatttt
     9001 ttcgctcggt ctctatgaac tgttcatcag cgacatcgat gaagccaaag gagaggcgcg
     9061 ctcgagccgt atcctgctga tcgaaagcct ggatgacctg aaggcacggc tggccaaagt
     9121 gatcctgatg attctgatcg tgacgctgtt ccaggaagcc atccgggtga aaatcgaaac
     9181 gccgctcgac ctgctctatc ttggcggctc cattgcgctt gtcggcctgg cactctatct
     9241 ctcgcacgcc tccgacgcca aaagcgaaca ctgatcctgc cccggcgggc ggataagctc
     9301 atccgccccc gggccgcgcc gccagtcatc gttgcctgac cggcagcact ccggccagcc
     9361 cggacatctt catacattct atatacggaa gtaacgatac tgtcacgcat cgttgttaac
     9421 ttacttgcac acatcctggc caggaagcat gatgtggcgc gagttttcgg ttcttgacaa
     9481 cacgctgacg ctgatgtgca taacctacgt tcccggaact ggctggctcc gggccaatta
     9541 ccccggcact gtaccggtct gaacaaggag cgccttcatg acccgactca tgcatgtaca
     9601 aagcaaaatc gatctgcttg ctgcgcgtga atacttcttc agcagcatca tcaaggcgct
     9661 gaccatggaa ccgctgacca tggacgttct gttcggcagt gatgccgatt atgcgccacc
     9721 gcggttgctg tcgatgaatg tggaattcgg ttcgctggtc atcgaagtgc aaccgaactt
     9781 catcggcgaa ggcgatgaaa gtcgctatta ctattccatt ctggtcatgg gggatatcct
     9841 gcgcgtcggc attttcatgc cgctttcaag aatcctgacc gtgccggtac aggatgatgg
     9901 acgtgagcgc ctgatccaga cctggaacgg cgcgcaattc aacgtgcagg atcgcggact
     9961 gggtatcctg tatgaatgga cgttcaccga tttcaacccg gccaactggg ccaacgccga
    10021 aaaatacgtg ctcgggatcc gttacctgca tttccgcttg cttgggctcg tgcacaacac
    10081 cgtgcgcgag gaaatcgcgc cgacgctact gcgcaactag acgcgaccgg acgcttcggc
    10141 gtcgccccac ccgggaccac gccgcgcacc gcagttcagg ccgagtggtt cggcacgtgc
    10201 gcctgttcgt acgcatgcag cgaagccaga cacttgcgca cgaatgacgg tcgagtgccc
    10261 gattcaccat ccatccagcg taacaacgtg gatttggaaa tgcccatggc cttggcaatc
    10321 gactggtgat catccttgtc aaaccccaga cgttcctgcc atttggcgat gaattcgggc
    10381 agcgaaccct ccatttcctc cacggcttcg aacaggtagg ggtgctcacc cgactcccgc
    10441 gcctgcttca gctgccggat cgactccagc accgcctgcg gaatcgagcg ggtgcggcca
    10501 tacacatagg tcatcatgcg cgaacgcgga atgcctgcaa tgcgcgccag atcgtcatgc
    10561 gtaagtccta gcgatttctg cagctcgagc agttcgcgct gatcctcgcg catcggctgg
    10621 atgcgcgtgc gtttacctgt tggccgcgta tcggtttcct gttcctctgc gcgttgacca
    10681 cccgaacgcg cccggctcag cgcctccagc accttgatct tggtggcgat gcgcgacgga
    10741 atcttttctg gttcaccgta cagcatgacc accatgcggg tgaggctgac cccgatcgcc
    10801 tgcgccatgg cctcatcgga cagattcagt ttgttcttga gattcttgag gttgtcctgc
    10861 gcgaccttgt tgggcgaata cgctccgtca gcggtggccg ttctggtcag cgactcaaca
    10921 ctggtttgat cgagaaattt attcatacac cgcccgcttc attcttgcca attggatcat
    10981 tgtagacgct tttgtcacaa cttggtagta ggtttatgta agatttgtgt atatgtaaga
    11041 aaaacgtcat gattcgtcag aatttttcct acacgcctca tggcagtggc gcattgagcg
    11101 cacgcagcac cggaacacca tacaattgcg cttgcgcaag cagcgcatcg cgttgtactg
    11161 ccggctcggt catgccctgt gcaaaccagt cgtccgccag cgaaacgatc gcctgcgggt
    11221 gaccacgaaa aaaactccac agcaacggaa acatcaacgt gtcttcataa cagaacgata
    11281 gcgcaagctc gtgctgccga agtatgacca gcccggtgcc aaaccagttc gcccgacatg
    11341 aaattttatc ccaggggcgc cacatgccga atggaatcgg tacgcgcgct tccagttggc
    11401 cgtgcagtgc accacggacc agcagcgcgt ctgcccagcg cccatcgctg cgcggtattt
    11461 cggcaccgag cagcaacacc gcatcctgcc ggcgtgcaag atccacagtc tgccgccagc
    11521 tatccgccac ccagggagcc cacaccccga ccgcagattc cggcagcaat accagcgttg
    11581 cacccgactt aagcgcagca cgcacccgtt gttcgagtct tgcctcgcgc agcaggatcg
    11641 ccatggcatc acgcggataa cgacccgagt gcgtgtccag cgccacccac cctggcggca
    11701 gtggcgctgt tgcagcgtgc aacggcagct gcgcccaggc actgagcgac agcagggcaa
    11761 cgcatctgcg ccagcgacgc aacatggcgg cccacggcga tgccgtacag gcgaacagca
    11821 gcagcatggc caccagcccg gccacagccg ttgcaggaaa ccaggaaccc gctgcaagga
    11881 ggggggacag ccagccgatc gcccccaggg gcggcaatgc ggtaagtacc aaggccaaca
    11941 tcaaccccgc cgggccgaac cggtacaaac cgccataggg cagggcgagc gcaagcgctt
    12001 gcgtgaccca caacagcgga ccgagaccag cagcggcggc ggggaaaaat cgcgtcaccg
    12061 ccagcggcgc ttcatgggcg catgccagat agtagcccgc catgaacgcg aacgcgccca
    12121 gaccgacatt cgccatacgc cagcctacgc tcagcaacag caacataccg ggccagttct
    12181 ggcgccagac cagcaggccg agcagcatgc cactggccgt cagcgcaccc acccggagcg
    12241 caaacagccc ctgcgttcgc gccatccgta acgatgtccg tgcccgctcc gtcattctcg
    12301 cccctaaccg aacgcatgtc gcaaattata tacaacaatt attgtgcgtt gcgcacattc
    12361 gatgcatcag gcgcactgcc ttgggttcac cgcggattcg ccgtacaatg cccgtttcgc
    12421 taccgtcgaa caccatgctg cgcatcaccg aaatcaagct tccgctcggg cacacgccac
    12481 aggcactcgc tgcagcggtc tgtcacgttc tgggcataag cgctgccgac ctgctcgatc
    12541 tgcatatctt ccgccgcggt tacgatgcgc gcaaacgcgc ccacatccag ttcgtataca
    12601 cggtagatgt cgcgctgcgc gacgaagctg cggtgcaggc gcgccatcgg cgcaacacgc
    12661 ggatcgggcc aacacccgac atgagttacc gcgttgtggc acaggcgcag caaccgccag
    12721 cccgacgccc tgtggtcatc ggctttggcc cggccggcat gttctgtgcg ctcctgctgg
    12781 cgcaaatggg gttacgtcca gtggtgctcg aacgtggcaa gccggtgcgc gaacgcacac
    12841 aggacacctg gaaactgtgg cgcaccgggg tactcgaccc cgaatccaac gtacagtttg
    12901 gcgaaggcgg cgccggcacc ttttccgacg gcaaactcta cagccagatc aaagatcctg
    12961 gctaccgcgg gcgcaaggtc atcgaagaac tgatccgcgc cggggcacct gaagaaattg
    13021 cctggatcag caagccacac atcggcacct tccgtctggt cggcatcgtg gaacgcattc
    13081 gccacaccat cgagcaactg ggtggcgaag tgcgtttcag cagccgggtg agcgacatcg
    13141 tgcgagaaca tgacgccatt caggcagtcg tcctcgatgg cggtgaggtc atcgctgcgg
    13201 atcatgtggt cctggcgatc ggccacagcg cccgtgacac gtttggcatg ctctaccggc
    13261 gcggcgtatt catggaggcc aagccgtttt ccatcggtgt ccgcatcgag catccgcaat
    13321 cgctcatcga ccgttgccgc ctcggggaat cggccggcca ccccgaactg ggcgcagccg
    13381 actaccgatt ggtgcatcat gcaagcaatg gccgggcggt atacagtttt tgcatgtgcc
    13441 cgggtggcac ggtggttgcc gccacatccg agcctggcgg ggtggtcacc aatggcatga
    13501 gccagtattc tcgcaacgaa cgcaacgcca acagcgcgct ggtggtcaat gtggaccctg
    13561 cggattttcc cggcgacgcg ctggccggca tcgatttcca gcgccactgg gaacagcggg
    13621 ccttcgaaga gggtgggaga aactaccatg cgccggcaca gcgtctggac gattttctgg
    13681 caggtcggcc atcaacccac ctcggtgaaa tcgaaccctc ttaccagccg ggcgtaacgc
    13741 ccgccgacct gtcggcctgt cttccggact atgtcaccca ggcgctgcgg gaagcactgc
    13801 cggccttcga ccgcgaaatg catggctttg cgcgaccgga tgcagtactg accggggtcg
    13861 agacgcgcac ttcgtcgccg gtacgcattc gccgcgacga tcacggtctg cacagcgtga
    13921 acacacgcgg gttctatcct gccggggagg gcgccggcta cgccggaggc atcctctcgg
    13981 ctgcagtgga cggcatccgt gtggcggaag ccgtcgcact cgatctgtgc ggtacgcctg
    14041 cgccgcactg atacagcggc gcgctgctgc cgctcaggcg gccggacggt tggtgtggtg
    14101 ccgggccagc accgtgcgca gcgtatcggc cagctcatcc ggagcgaact tggccacata
    14161 gccgtcggca ccgacgttac gcacgtgatc ctcgttggcc gcaccagtca gtgaagagtg
    14221 gatcaccacc ggaatgtcgc ggtaacgctt gtcctgcttg atgttgcgtg tcaaggtgaa
    14281 gccatccatc tccggcattt ccagatcggt cagcaccagc gccacacggt cgtgcgcgct
    14341 gccgccttcg gattcggcca gacgggtgat ctcctgcaat ttgtcccagg cctccttgcc
    14401 ggtcttggtc atgatgtaag gcacacccat tgccgccagc ccctgctcga tgagcgaacg
    14461 cgccaccacg gaatcgtcag ctgcgaggat gatgctgcca ggcttgatcg tgaccttggg
    14521 cccgatggtt tccgcattca ggttatcttc cggcggcatc aggttgcgca agatggtttc
    14581 cacatccagc acctgagcca gacgggaatt gtccacctcg ccatcgagac gggcaatgct
    14641 ggtgaccatg ccatcggcat tgctggattc agcggagagc acctggttcc agtccagccg
    14701 gacgatgtcc tccaccgcct cgacggcaaa cgcctgggtg gtacgcgcat attcggtgac
    14761 caggatgatg ttgagaccgg tcttgggtgt gcagccgacc accgccggca ggtcgatgac
    14821 cggaatcacc tggccacgca ggttgacaac gcccagcatg tgctgcggtg cgcccgcgac
    14881 ggcggtgatc tgcggcatgg ccacgatttc acgcaccttg aacacattga tgccaaacaa
    14941 ctcggaatgg ttaccgctgg catccccccc caggcgaaac agcaacagtt cgaacttgtt
    15001 ggtgcccgcc agattcgtgc gttcatcgac ttcctgctgg actgatttca cttgcccctc
    15061 ctggtgataa actgcaggtt cattattgtc ttgaggcctg gtccggcaga cgctccctta
    15121 cccaccgccc ctgaccggac gtgttgcaca cacacgcacc gtcggagtgc ccaaccccgc
    15181 ccgccaggct aatcggcggc atcccgagcc agggcaaccc gcgcccgtgc gatcgccgca
    15241 cgcacctgcg tcggtgccgt accccccaca tggtcccgac tgtgcaggga tcctgacagg
    15301 gataatacac cataaacctc ttcgccgatc aaaggcgaaa acgcctgcaa ctcggccagc
    15361 gccagttgtg ccagttcgca gccacgttct tctgccagcc gcaccgcgcg tgccaccacc
    15421 tcgtgagcat cgcgaaatgg cagccctttg cgcaccagat agtcggccag gtcggtcgcg
    15481 gtcgcgtggc cctgacgcag cgccgacagc atcgcctcgg gttgcacgcg aatgccggcc
    15541 agcatgtcgg cggcgatctc cagcgtgtcc agcaacgtgt ccacggtgtc gaacagcggc
    15601 tccttgtctt cctggttatc cttgttgtaa gccagcggct gacccttcat cagcaccagc
    15661 aacccgagca ggtggccatg cacgcggccc gtcttgccgc gcatcagttc cggcacatca
    15721 gggttcttct tctgcggcat gatggaagag cccgtgcaga accggtccgg cacgtcgatg
    15781 aaaccgaaac gcggattcat ccacaggatc agctcttccg ccagccggga gaagtgcgtc
    15841 atcaccaggc tggcgcaagc gcagaattcg atggcgaaat cgcgatccga tacggcatcg
    15901 agagaattct cgcacacccc gtcgaagcca agctcgcgcg ccacccattc gcgatcgatc
    15961 ggataccctg tgccggccag tgcagccgcg ccaagcggca gccggttcat gcgggcgcga
    16021 caatcttcca gacgcccggc gtcacggctc agcatctcga agtaagccat catgtgatgc
    16081 ccgaacgtca ccggctgcgc cacttgcaga tgggtatggc cgggcatggc cacttcggcg
    16141 gtacgctcgg ccagatcgag cagcgccgaa cggcaggcat gcagtgcgtg ctggatgcga
    16201 tcgagcccgt gacgcaaata cagccgcacg tcggtcgcca cctggtcatt gcgcgaacgg
    16261 ccggtatgca gacgcttgcc cgcgtcgcca acacgctcgg tcagcgcccg ttcgatgttg
    16321 aggtgtacgt cctccagttc cagtttccac tcgaaatggc cggcgcggat ttccgccagg
    16381 atttcaccca ggccgcgctc tatggccacc agatcgtcag cagacagaat gttttgccgc
    16441 gtcagcatgc gcgcgtgcgc cagcgaccct tgtatatcga attccgccag acgataatcg
    16501 aaaccgatcg aggcggtgta gcgttgtacg cgttcggcga cgggctcact gaaacggcca
    16561 gaccaaagtt gcgataccgg atgggacatg acgaggatcc gtagtgatga atgacgcgcg
    16621 cacgcggcag cgcaatcccc gcattatgcc aaagccggtg caagacggct atcccgacca
    16681 cttgccgaat ttctgcaatc tgggagtgat gctgcgcatc ctgctgctcg tggaagccct
    16741 gggctgggtg gcggcctggc tgcgctcgcc cacgctggca gcggcatggc aggcggtgct
    16801 cgatctgtca ccgccgttgc agggcagcct gctgctcacc ctgcttgcgc tgtgcccggg
    16861 tcgtgcctgg ctgacacgtc tgccctatgc cgccggagtg gccgccacgc ttgcaatcgc
    16921 gcttggtgcc acagcactgg tactgcgcgg catgtcgcag ctgttcccgg ccgcaggctg
    16981 gtcgatggat ctgcacactg ccatcaatgt catcatctgt gtcgcgctgc tgatcggcta
    17041 tttccgtctg cgtgaccggg cgctgtcgcc ggcactcacc gaagcccgcc tgcaggcgtt
    17101 gcaggcgcgc atacgcccgc acttcctgtt caacagtctc aatgccgtac tcagcctgat
    17161 ccgcgccgaa ccggcccggg ccgaacgcgc gctcgaagac ctggccgaat tgtaccgcgc
    17221 catgctctcc gacccacgac aactcacgcc gcttgcgcag gaagtcagcc tgagccgaca
    17281 gtatctcgaa ctggagcacc tgcgcctggg cgaacgcttg cgcatccagt ggcatgtcga
    17341 caaaatgccc gccgatgccc tggtgccccc gctgctgttg caaccgctac tggaaaatgc
    17401 cgtctatcac ggcatcgaac cgttgccgca gggcggggag atcctcgtca atatctatgt
    17461 gcacaatggg gaattgcacc tcgtggtgcg caatccctgc catcgcagcg gacgccgcgg
    17521 cggcaacaac atggcactgg aaaacatccg caaccgtctg atgctacact tcgacgtcga
    17581 ggccagcctg cacaagcagg tcagcgatac cgaataccag gtgcacatca tcatgccttg
    17641 ccggaggaac gcccatgcct gaacccttga gggtgttcat cgtcgatgac gaactgcctg
    17701 cgcgcaaccg cctgcatgac ctgctgaacg actgcacgaa ggcgttgccg ctcacggtat
    17761 gcggcgaagc ggccaatgcg caaattgcgc ttgagcggct gcccgacctg caagccgacg
    17821 tggtgctgct cgacatccat atgcccggca tggacgggct ggaactggca cagcacgtcc
    17881 gcctgctgtc accgccgccg cagatcatct tcatcacagc ctacgatgcg cacgccatcc
    17941 aggcattcga ggtcgatgcg cgcgattatt tgctcaaacc cgtacgcgcc gagcgtctgc
    18001 tggcggccct gcgcaaggcc gatcaccagc gcgcagcgcc cgccacaccg cggttgcgca
    18061 tcatcgaccg cggcgaggtg cggctggtgc cgattgcgga aattctctgg ctgcgtgccg
    18121 aacagaaata catcaccgta cacacgcgca tgcgcgacta cctgctggaa gacagcctca
    18181 cgcgactgga acaggcctgg ccgcagcaac tggtgcgcat ccaccgcaac gcgctcgtga
    18241 cccggcacgc cattgctggt ctgcgcagcg ttccccccgc tgcgggagaa accgaatcgg
    18301 gtatggaaat cctgctcacg ggggaagcgg ccggattgcc ggtcagccgt cggctgcaac
    18361 cggtcatccg ccaactggtg cgcactcagc agtaaccgcc ggccgcgccg ttatccggct
    18421 gccgtcttgc gtgcacgcgg acgtgccttt gcagccgcag gcgcagcggc aggtttcttg
    18481 cgcgtacggg tggtcttgct gacggcggca cgcgccgcca gcagcggcag tacatcgtca
    18541 aggcctacgg tggcgctgtc aagcgattcg ggcaagctgg cattaacctt gccatgcttg
    18601 gcgtaccagc cgtagcgccc ttcgtgcagg gtcaccgcct cgccatcatc cgggtgtttt
    18661 cccagcggcc gcccggcaaa cgcctgtcca cgcggggtac gttcctggcg caagatgtcc
    18721 acggcgcgtt ccaacccgat ttcgtacaca tcgtccgcct tgggaatgga tttgaacttg
    18781 ccatcatgct gcaggtacgg accgaaacgg ccgatgttgg ccaccaccgg cagaccggta
    18841 tcggggtggt tgcccagcgt gcgcggcagc gacaacaggc gcaacgccgt agcgagatcg
    18901 gcctgctcga ctggcagatg cttcggccag ctcgcccgac gcggcttgac cgtcttgtct
    18961 tccggcgtgg cgccgatctg tacgtaccat ccgtagcgac cattgagcag catgatctcc
    19021 tggccactgg ccggatcaac gcccagcgcg gccggttcgc gcgagccggc tccagcatgg
    19081 aacgggcgcg tatagctgca ttccggatat ccggagcaac cgatgaacaa cccgcgcttg
    19141 ctggcctgaa ggaacagcgg ccggccacat tccggacaga cttcgtccgg aaccgggcag
    19201 ccgcgctcga cctcggcctt ggccgccagc gtgccggaga attcatgcca gaactcgtca
    19261 agcagcggca ggtatttgcg ttcaccattg gcgacatcgt cgagtttgtc ttccagtcgt
    19321 gccgtgaagt cataatcaac gtaatgggtg aagtgcgtgg tcagaaagca gttgaccagt
    19381 cggccggtcg gcgtgggcac gaagcgtttc ttgtccaggt cgacatattc acgctcctta
    19441 agcgtggaaa tgatgctcgc ataggtcgat ggccggccga tgccatattc ttccagcgtc
    19501 ttcaccaggc tggcctcggt gtagcgcggc ggcggctggg tatgatgctg ttcgccgtaa
    19561 atgcggtcca gtggcacggt ttcgccggtt tccagcgccg gcagccgggc ttcttcttcc
    19621 tcggtaccgt catcctgatt ttcctggtag accgcgatga accccgggaa cgcgagcgtc
    19681 tgaccactgg cgcggaacac gttgccagcg tcggacacgc tgaaatcgac gctcacggta
    19741 tcaaactgag ccgaggccat ctgacacgcc agcgttcgct tccagatcat ttcgtacagg
    19801 cggaactggt cggccgtgag aaaagcgcgc atggcgtccg gtgtgcgcgt gatcgaagtc
    19861 ggacgcaccg cttcgtgcgc ctcctgcgca ttacgcgatt tgttacggta gtagaccggc
    19921 ccttcgggca gatatccggc atcgaaacgc gactggatgt actggcgaat ctcgataatc
    19981 gcttcggcgg ccaggtgaac cgaatccgta cgcatgtagg taatcagccc cgtagcccca
    20041 ctgccaatgt cgatcccctc gtacaactgt tgcgccgtgc gcatggcccg gtcagtggtc
    20101 ataccaagct tgcgcacggc ttcctgttgc aaggtggacg tggtaaacgg tggtgccgca
    20161 tgccgcgatt tgcgtttgcg ttcgatatgc gtcacgcggg cactgtcctg cgtggccagt
    20221 tcgtcgagca cggcctgctg gctgaccatg tcaggcagac tgaattgttc cagccgcgtg
    20281 ccatgccatt gcaccagacg cgcagcgaac gcctgtcgcc ccttgtggct gtcgaaatgg
    20341 atcgtccagt actcctgggc gacgaaacgt tcgatttccg cctcgcgctc gacgatcagg
    20401 cgcaatgccg ggctctgcac ccgaccggcc gacagccccg ggctgatctt gcgccacagc
    20461 agcggtgaca ggttgaaacc caccagataa tccagtgcgc gccgcgcctg ctgggcatca
    20521 accagcggca tcgacacctg gcgcggatgc tcgaccgcct gacggatggc gctggcagtg
    20581 atctcgtgaa actcgatgcg ctgcagcgga atgcgtggca gcagttgttc ttcgcgcagg
    20641 atctccgaca gatgccatgc aatcgcctcg ccttcgcggt ccgggtcagt ggcgagcagg
    20701 atgcggtcgg ccttgcgcgc cgccttgagg atgctcgcca catggcgttc attgcgctcg
    20761 atgatctggt atttcatggc gaaatgctgc tcgggatcaa cggccccggt tttcggcacc
    20821 agatcgcgca cgtggccata gctggccagg atctcgtact cctcccccag atactttttc
    20881 agcgtcttcg ctttggaggg agattccaca atgacaagcg cagacatcgc tcttcctcaa
    20941 tgcagggtgg gcgctgcgtt ctgattgaac agcatgtctt cgagcaacag gaagttctgg
    21001 tcgggatgat ggctccacag gaccaccagc gtgatccagc gaatcttttc agcgatgttg
    21061 tcatcctcgt cgagcgccat gacacgatcg atgatccatt cccgctgcag atcgtcgatg
    21121 atgcccgact gctgcaggaa caacaggaag ctctgttctt cgacaccgag ccgcaactgc
    21181 tcgtcgctgc tgaaacagcg cagcgcatcg cgcccgccgg ccagactgga aggatagtcg
    21241 tctccgtgaa tcccgtcgag ttcgcccagc cagtcgagca cctgctcgat ctcgctcagc
    21301 tgaaaaccgg cagcattgag cttgatggcc agcgtatccc gatcgggtgt cgactcgatc
    21361 tgcgggtaat tttcgaacag gtataacagg atatcgaaca tcttcgtcca tcaggtaacg
    21421 cgttggtata gtccaccggg cagacaggcc acctgtccgg caagttctct ttccaacagc
    21481 atggcggaaa ctgtttgcgc cgtcaacccg ctgcgttcta tcagcacatc gatcgccacc
    21541 ggtgcatgac ccatggcctg ccacaacaca tcatcgccgt cgacaccggc gtgtgctgtc
    21601 gtctctcgtg ctgcaggcgc cgtggccata tcaccccagc gcaactcttc gagaatgtcg
    21661 cgcgcggctt cgaccagccg tgctccctga cggatcaggg catgcccgcc acgcgacagc
    21721 ggcgaatgta tcgaaccggg caacgcgaac acctcgcgcc cctgttcggc cgccagccgt
    21781 gctgtaatca gcgaaccact gttgagcgtc gcttccacca ccagacagcc gaggctcaac
    21841 ccgctcagga ttcggttgcg ccgggggaaa ttggctgcat gtggtggtgt tccgggcgaa
    21901 aattcgctga tgattgatgc ttcttcggcg atgcggcgcg caagcaaccg gttgcttgcc
    21961 ggataaacca tgtccggtcc ggtaccgatc accgccaatg tggcgccgcg tccagccagg
    22021 ccgccgcggt gggccgcagc atcaatgcca agcgccagtc cgctcacgat cagcaggccg
    22081 gcgtcagaca gttcgcgcgc aaacgcctcg gcatcgcgca caccctgtgc gctggcgttg
    22141 cgactgccca ccaccgccag tgctgcgcgc gccggcaggt cgcgccggcc gttgacgaac
    22201 agcatgacgg gcggatcggg aatttgcagc aacaggggag ggtaatcggc atcggccagt
    22261 gtgaccagat ggtggtccgg atgctcgagc cagcgccgcg aagcatcgat gcgcgcctcg
    22321 acgacgccgt tggcgatatc acgcgcgacc gcctcggttg ctaccgtggc cagcagggca
    22381 tacggcatgg cataaatggc ctgcggaccg ccgaagcgtg ccagcagcct gcgcatgccg
    22441 acactgccca gtcccggcac ctgacccagc gcgagccagg cgtcgcgttc cgcggctgcg
    22501 agcccggaat caggggttgc gcacaatgtc gagcgcgctt accgaatgcg tcgaatccat
    22561 gatcagcgcg tacgccagct tgtcatagac ccgatacacc atgcacagcg cgctgcgcgt
    22621 ctctggcatg gtcatgccgg ccaccgcacc agggccatgc cggtaaatcg ccagcacgtc
    22681 gccctgttcg agcccgtcgg cacgtccctt gttgatgacg atactgttga accgaccggc
    22741 ctcgctcatc actccgatgg tcgacacgat caggccatcg attttctggt ccggggcatg
    22801 cggcacatag ttgaacgtat aggtttcgcg cgccggccac ggcacgaggc gatcgccatc
    22861 ctcgacttcc ttggcagacg acacgatcag cacgcgttgc ggctgacccg gcgtgacggt
    22921 gcgcccttcg cccaggtaat ccgcgccata accgagcaca cgcttgccct tggcgtcagc
    22981 gggatcgatg atcggtgcgc ctggcgtcac gatcttccag cgcgaccgcg cctcgccctc
    23041 acccgtggca aacacttcgt caccagcacc gacatctata tgggcgcttt cgaattcgac
    23101 gatcctgggc agatcatgca tttctgcctc atccatcacc accggctggc tggcaaacgc
    23161 atggatggca ctggccggca gcggcggaat gccaccgttg tcgtcgagcg actcgccgcg
    23221 caccgaaggc gacagatgca ccgtaggcac ggcgtcagga ctctgttcca gcgagaggcg
    23281 cggctggccg ttgacccatt gcagcacaat cacattgccg ggatagatcc agtgcggatt
    23341 gtgaatctcc tcacggttca tcccccatat ttgcggccat ttccatggat ccttgaggaa
    23401 atgtgcggaa attccccaca gggtgtcccc cttgaccacg acataacgct ggggcgcatt
    23461 atcctgcagg cgcagatcat cggcgtgtgc ggcaaccgcc acccatccgg caagaaacag
    23521 agaaacaatg acggccttca tgcaactccc tcgctactgg tatgggcagg ctggcgaaac
    23581 gggtgatacc attgtaccct cgacatacgg atgcaacatt cgtattacca accgaaatcg
    23641 acagttgctg tggattttgc atgtaattgt ttatgtaggc aagtggttat ggctaaattg
    23701 acgattttgc gctatccgga tccgcgcctg tacacgcgcg ccaaaccggt tgcggccgtg
    23761 aacggacgta tccggacact ggcagccgac atggcagaaa ccatgtacgc cgccccgggc
    23821 atcgggctgg cggccactca ggtggacgaa cacatccagt tgctggtggt cgacatcagc
    23881 gaaacgcaga atgacctgca tgtattcatc aaccccgaac tgttatggac acagggcagc
    23941 gccgaacgcg aagagggctg tctgtcggtc ccgggcatct acgacaaggt cacccggccg
    24001 gagcgtattc gcgtgcgcgc gctcgatctc gacggcaaac cgttcgaact cgaggccgaa
    24061 ggcgtgctcg cagtatgcat ccagcatgaa atggatcatc tgcttggcaa agtgttcgtg
    24121 cagtacctgt caccgctcaa gcagacgcgc atccgcaaca agctgaagaa acgtttgcac
    24181 gaaaccatgt aggaatgacc atgcgcttca tttttgccgg cacgccagaa ttcgcccggg
    24241 tgacgctgct ggcactgctg caggcgggcc accactgcct tgccgtactc acccagcccg
    24301 accggccttc ggggcgcggc atgaaactca tgcccagtcc ggtcaaacag gcagcgaccg
    24361 ggctgggcct gccggtctgg caaccgctta cgttgcgcga cgccacagtg caagcccggc
    24421 ttgccgcgct gcaggccgac gtgatggtgg tggcggccta cggccagatc ctgccacagg
    24481 cggtgctgga tctgccgcgc gcaggctgcc tcaatatcca cgcctcgctg ctgccacgat
    24541 ggcgcggtgc ggccccgata cagcgcgccc tcgccagcgg cgacgtcgag accggtgtca
    24601 gcatcatgca gatggacgcc gggctggata ccggtgcggt cggcctgctg cgcgccatcc
    24661 ccatcgatcc aggcgacacc gccggcacac tgcacgaccg gctggccgcc ctcggcgcag
    24721 cggcgatcgt cgaagcgctg tcgttgctgg aacacggtcg ctggcggcca caggcgcaag
    24781 ccggggaggg cgccacgtat gccgcaaaga tcagtcgggc cgaagcgcaa ctcgatctgc
    24841 gccagccggc agagcagctg caacgcctca tacgggcttt cgaccccgtg ccgggcgcct
    24901 tcgtgcaaca ccacgccgga cccctcaaga tctggcaggc gcaatgcact gccggccacg
    24961 gcgaagcggg ccgcatcctg catgcgggtg ccgacggcat cgtggtcgcc tgcggcagcg
    25021 gcgccttgcg catcacccgg ctgcaggcac cgggcggcaa gcggctcgat gccgcccagt
    25081 ttctcgccgg gcacccgttg cacgcgggcg aatggctggt tcagcccgca gccgcagata
    25141 ccccggccag ccagcgctcc agctgatcga gcaacccgct ttcggcgcgg tcaaacgctg
    25201 ccgcggctcc cgcagcatcg tagcgcgcca gcggcgcagt ctgggtaaat tcggctcggg
    25261 caagcaaccg gtgcgaagcc acgtcgtaca gttcagcgcg cacacgaatc accgcctgtc
    25321 ccggctgcgc gcgggcatcg tggtaaaacg ccagcaaggt ggtcgtcagc cggcgctgcg
    25381 cgctaccgtc ccccgaacca tcgagcacag tcgcatacaa ttgactggcc gccagacggg
    25441 caaacagcag atcgctcatg cgcgtgctcg gcagttcgct ccactgggca tagcggtagt
    25501 gtccacgcac gccgggcgcg cggctgaaca ccaggtccgg attgtcgtca tagctgttgg
    25561 agcgggtggc ctgcacacgc agcacttcgg gccgggttgc atgtggcgcc agcacgcccg
    25621 gcatccggtc ttccagcaca tacacctggt gcaccccatc acccagactg gcacaggccg
    25681 ccagcagcaa tacgctcagc agcaacaatg ttctgctcat gtcattctcc cggcgcggtc
    25741 tcgtcgcgcc ccgtgccaaa aatcagcgaa cccggattgg acaaccgccg ggaggtgccc
    25801 gtgagcgccg tttcaccctg atgcacatcg ccgccgatct ggttgagccg gtaatcggcg
    25861 gtatcggtca accgctgcag ctcgcgcgac agaatcaccg actgctgctg caaggtatcc
    25921 agcgcctgcc cggcctgtac cagcgtgtga ctggtctggt gcgtcagcag cttcagatcg
    25981 ctgcgggtct cgtcactggt caccccgaca tcatcggcca tgtgcgtcag ggaatcggcg
    26041 gccgcacgaa aactgccaag agtgtcatcc agttcccggc tgtggcgggc cagctgcgcc
    26101 gacacctgtt gcaggttatc cagcgtatgt gccagcgcat cgcggttgcg ttgatcgagc
    26161 acctgattca ggttatcgat cagttcggcc gtcttgtcgc tcatctgggt cagattggtg
    26221 gtcacgcgtt ccaggtccga actgccctcg gcaatcaccg gccagggcgg ggttgccctg
    26281 ccgggcatca gcggactgtc ggaatgcgga ttgtcgatct cgatcgacgc cagtccggtg
    26341 acgatattgc gctgtaccgt ggcatggctg tcggcatgaa tcggcgtccc cggctcgatc
    26401 ttgatgttga cccgaacggc ttcgcggttg ccgggaacaa acgcatagtc catgacttcg
    26461 cccaccttga tgccgcgaat tttgaccgga ctgctcgggt ccagcccgtc gagagattcc
    26521 ttgctgaaat aaaccgtgta atgctggtag gcgatgcgct cgccaccggc cagccagaca
    26581 aggctggcaa gcagcgccag gctgatcgca acgatcacgc tgccaaccag aatgtatctc
    26641 gcttcgtttt ccatgttcgg cctcaggaga gctgggttga tggagttgcc ggtgcgacct
    26701 ccggcgtccg gctggaaaaa taggcgtgca gccacggatc atccagcgca cggatctgat
    26761 ccagcgtccc cgatcccagc accttgccct gtgccagcac gatgatgcgg tcggcgatcg
    26821 acagcagggt gtccagatca tgcgtcacca gcagcaccgt caggccgaga ttgtcacaca
    26881 gggtgcgcaa caaccgatcg aacgcgcgcg cggacaccgg atcgagcccc gacgtcggtt
    26941 cgtcaagaaa cagcagctcg ggatcgagcg ccagtgcacg cgccagggca acacgcttgc
    27001 gcatgccacc ggacaattcg tccggcatct tgcacgcggc atcaggcggc aagccgacca
    27061 tcgccagctt gagtcgcgcc agctgcaggc aatcgctgcg cggcagtcgg gtatgctcga
    27121 tcagcggcgt ggccacattc tgttcgacgg tcaacgagga aaacagcgcg ccatcctgaa
    27181 acagcatgcc ataacggcga cgcatggcgt tgagcgcgtc gctgtcgact gcccagacgt
    27241 tgccgccaaa cagcatggcc tcaccggccg tcgggcgcaa caggccacac aatacgcgca
    27301 acagcaccga tttacccgta ccgctgccgc caatcagtgc cgcaatctcg ccacgccgca
    27361 ccgacaggct cacgccccgg tggatgtact gaccgcccag ccgggtctcg actgcgcgcg
    27421 tttcaaccag cacctcctcg ccggcgtgcc acatgctcag atccccagct caaccagcgt
    27481 gaccgcgatc accgcattga gcagtatcac cgccacgatg ctctgcacca cggtcgaagt
    27541 cgtggacagg ccgatgctgc gcgcgttgcg ccgcacccgc aggccggtct ggctggcgat
    27601 gaggccgatg aacagggcga ataccggcgc cttgcccagc ccgacgacca cggtggccgt
    27661 atccagcgcg ctgaccatgc ggttgacgaa cgctgctggc gcgatatcga gctgcttgtc
    27721 ggccaccagg atgctgccgc cgatgccggc gagatcgcca ataaacacca gcaacggcag
    27781 cgccatgacc agtgccagca gacgcggaat gatgagcacg ccccacggcg acaggccaag
    27841 cacgctgatg gcgtcgatct cgtcatcgaa cttcatggtg cccagttgcg cggtgattgc
    27901 cgaacccgaa cggccggcga tcaggatcgc caccaggatg ggtgagagtt cgcggcagat
    27961 ggccagcgtc gtgccatcga ccacatacag attggcgccg tattgcagcg cctgccggcc
    28021 cagcaggtac gcaaacacca caccgagcag gaacatcatc atcaccacca gcacgatggc
    28081 ctgcacgaat atcgcatcga cctgagcggt gaactccttg atgcggaact gctgcgggtg
    28141 gcgtatcaga ccgacgcatt ccgcacccac gcgaccgacc agcgacaatg ccgcgccgac
    28201 gtattccgcc agccggtgcg cggcagcgcc gatctgcacc acccagttgc tggcgggcgg
    28261 ccgcatgcgt tcactggggg tgaggttttg tcgcaccagt tcgagaatgg cacgcgccgc
    28321 cggatgaaaa ttgaccggaa cgatggcgtc cgccgtcacc cctgcggccg caagggtgcg
    28381 caacagcatc cacgcgccac tcggatcaag ccgttcaatg ccatcgccat cgagttccac
    28441 ggattggggc aggggctgcg gctgccacaa cgggcgacgg tccatgcgca ccagccgcca
    28501 gtcgccgctg gcgataaccc gcgccggacc cgccgaatga tcgagccgca gcgtgggtgg
    28561 attcaccgcg gcacccgctc gaaccggtag accgcgagac tgccggccag attgggcagc
    28621 cagcgcaccc gccgaccgcc agtcatcacc acctgttcca gaatatggat gccgagcctt
    28681 gcgcacagtg tctcgaaatc ttccatggtg cacaggtgca cgttcggcgt gtcataccat
    28741 tgatagggaa tgtccgccga taccggcatg tggccacgca gtacctgcca gcggttcttc
    28801 cagtaaccga aattgggaaa ggtgacgatg ccttcacggc cgatacgcag catttcgcgc
    28861 aggatcgcct cggtgccacg cacggcctgc agcgtttgtg acaacaccac ataatcaaag
    28921 ctgtcggcag cgaattcggc caggccgcct tccagatcgc gctggatcac gttgacgccg
    28981 ttgcggatac agcccagcac gcgctcggga gcaatctcca cgccataacc acgcacgccg
    29041 cgctgttcgt gcaggctgcg cagcagctct ccgttgccgc accccaggtc cagcacctgc
    29101 gaaccggggc ggatccaacc ggcaatggtc tggaagtcct gtctcatctc acacgtctat
    29161 cctgtccatg taggctcgca ccagtgcatg gtaatgcgca tccggcatga gaaacgcgtc
    29221 atgcccgtgc gccgaatcga tttcggcgta actgacattg cgcccgttat ccagcaacgc
    29281 atgcacgatc tcgcgtgagc gttgtggtgc aaagcgccag tcggtcgtga acgaaatcac
    29341 cagaaagtcg gccacagcgg cggtcagcgc ccggctcagg tcgcccccgc ccaggcgatg
    29401 cgcggggtcg aaatagtcga gcgcgcgcgt catgagcaaa taggtgttgg cgtcgaacga
    29461 atccgcaaac ttgtcgccct ggtagcgcag gtaggattcg atctcgaatt cgacgtcata
    29521 gccataggaa aacgcgccat tgcgcagaat acggccgaat ttgctgccca tcacgtcgtc
    29581 ggacaggtag gtgatgtgcc ccagcatgcg cgccagccgc agtccgcgcc ttggcgtcac
    29641 ctgccgggcg taatagtcac cgccatgaaa atccggatcg gtcacgatcg cctgacgggc
    29701 gacatcattg aaggcaatat tctgcgcagt caatcgcgga gcggcagcaa tcacaaggca
    29761 atggcgcagt cgccccggat ggctgatgct ccattgcagc gcctgcatgc cacccagcga
    29821 accacccatc actgccgcaa actggctgat gcccaggcgg tcggccagcc gtgcctgcac
    29881 cgccacccag tcctcgaccg tcaccagcgg aaagcgcgaa ccccatggct gtccagtgac
    29941 agggtcaatc gacgcagggc cggtcgaacc gtggcagccg ccgggattgt tgacgccgat
    30001 cacgcagaat ttgcgcgtat cgaccggttt gcccggaccg atcagattgt cccaccagcc
    30061 aggttttttg tccgcaggac tgtgataacc cgcgatgtga tgattgccgg acagcgcatg
    30121 acacaccagg atggcattac tggcccgggc attgagttcg ccgtgcattt catagaccag
    30181 ttcgtaggca ggcaactgcg caccggactg caatgacagc ggtgtgtcga atcgggcggt
    30241 ttgcggcgtg accagactgg cggggtcgat gacaacggtc atatgcacgg tctcatgacg
    30301 cgatcagtcc tcgaaccgca cggtgccgcc accttgtgcg gccaccatgt gcgccaggct
    30361 ggcaaacagt tcgctgccat cgcgcgtgtt gcgccaggcg cgaccatcat ggcgatagtg
    30421 aaatccgccc gagcgcgcgg ccacccagat ctcatgcatg gccggctggc ggttgacgat
    30481 gatctttccg tcggggcatt cgatctccag cacattgccg gctgccagtt cgaaatcgac
    30541 atcgccttcg gccagttcga gcgcgccttc cagccgccgg aacgcttcct cggtgagctg
    30601 gttaaattcg gtttctgtca tgatagactg cgttcgtcaa atcaaaagtg atcaaggata
    30661 gccccatgcc ttccattctt cgcctgctca tgatgctttt cctgatgctg ttgctgcacg
    30721 gctgcggaac ccagggtccg ctgtatcttc cccaaaatcc cgcccaaacg caagagaatc
    30781 agccatgaca ccacccgatc tgtccccgtt tgaccccgcc cgcctggaag ccatcgccga
    30841 gcgctgggac acaccctgtt acgtctatca cgaaggtctg ctgcgaaaca ccttccaggc
    30901 attcagcagc gcgctcgaag gtcggccaca ccgcctgtgc tatgcagtca aggccaattc
    30961 gtcgctcgcc atcctgagtc tgttcgctcg catgggcgcc ggtttcgaca tcgtctccgg
    31021 cggggagctg caacgtgtgc tggcggcggg cggcgatgcc ggatccgtgg tgttttccgg
    31081 cgtcggcaag cgtgccgacg aaatccgtct ggcgctgcag gccggcatcc tctgcttcaa
    31141 cgtggaatcg gaagccgagc tgctgcgcat cgaccgcatc gcaaacgaaa tggggctgcg
    31201 cgctccggtc agtctgcgtg tcaacccgga tgtcgatgcc ggcacccatc cttacatttc
    31261 caccggactc aagaccagca agttcggcat cgcctatgac gatgcgctgc gcctgtacca
    31321 gcgcgcggcc agcctgtcag gggtccgcgt cgttggtatc gattgccata tcggttcgca
    31381 gatcactgaa atcaccccat tcatcgatgc gctcgaccgc atcctgctgc tggtcgacat
    31441 gctgacgagt gttggtatta tgctacacca tattgacctg ggcgggggac ttggcatacg
    31501 gtaccacgac gaaagccctc ctgcgatcgg tgattatgta cacgccattc gcagcaggct
    31561 ggacgggaga ccgcagcatg tgctgctgga acccgggcgc gctctggtcg gaaacgccgg
    31621 gttgctgctg acccgggtcg agtacctcaa ggccggagag gaacgcaatt ttgccattgt
    31681 ggacgccgcc atgaacgatc tgatgcgacc cgcgctgtac gatgcctggc acgagatcgt
    31741 gccggtgcgg ccgcgcacgg cggggcggat caactgggaa gtcgtcgggc ccgtgtgcga
    31801 aaccggcgat tttatcggtc acgaacgcgc acttgcgcta caagagggcg acctgctcgc
    31861 gatactgtcg gctggggcct atggtatgtc gatgagctcg aattacaata gccggccgcg
    31921 cgcagccgag gtattgctgc gcgacaatgg agaaaactgg ctgatgcgtg aacgcgaacg
    31981 catcaccatg ctgtatgcga acgaacatat cccggattaa atggtttttt cggttgatgt
    32041 gagaaaaaac ttatatttga atccgattat attggttcgt acaatgacca atcgtgaacg
    32101 cgcaggcagt ctgcgcgacg aagcaaggga ggtgtcacat gagtgtcttg cataaaaaag
    32161 caacaggcag catcgggtcg caaccgcgcg gaaggggatc ccgtctcggg ttcgaccgtg
    32221 cggacaccca gatcacaacc atgcgctgga tgacggccgg actgctggcg gcatcgagcc
    32281 tcgccgtatc gctcgacgcg tcggccacgc caatttttgc ccgtcagacc ggacagaact
    32341 gtatggcttg ccacgccggc ggccaattcc ctgaactgac gccctatggc cgtctgttca
    32401 agctgaccgg cttcacgctc ggcaagcgca caccgatccc gctggcagta atgggcgtat
    32461 tcggttcaac gacagttggc ccggcacaaa ccccggctcc cagttctccc ggacacaccg
    32521 caaccgatgg cacaccgacg ttctacaccg gcagcatctt tgctggcggc aaaatcaccg
    32581 acaacatcgg tatgctggcg caatacacct ggggcaacta taacggcagc aatggcgcct
    32641 cttttggtgg tggctccgac aacaccgaca tccgttacgc caaccgcgtc atcaacaaca
    32701 acaacgacat catctacggc gtctcgctca acaacaaccc gggcgtggaa gatgtgtgga
    32761 acagctcgca gacctggggc tacggcgtcg tgccgggcag taccggatcg ccagccggct
    32821 acaccatcct cgacggcggg cttgccgccc aggctgtcgg tgctgacgcc tacctgtact
    32881 ggaacaaggc catctacgcc gaagtgggcg cctaccgtac cggtaacggc gtgttctcct
    32941 tcctgacgca aaacaatggt ggtcaggctt atctggccaa caccagcccg tacgtccgtc
    33001 tggcctacac ccacgagtgg ggcgcgcaaa acatcgaagt cggttatacc ttctttgacg
    33061 ccgacgtttt cgaaggaacg gacaccttag gcaacaccta caccaattcg ggtccatatg
    33121 acgagcaccg cgacaatgcg ttcgacttcc agtaccagta tctgctcgat ccgcataccg
    33181 tcaccttcga gttttcgcgc atcaacgaag tgatcaaccc gaacggtgct ggcgcagtca
    33241 acttcaccgg cgctctgggc cagggtcagg tcaacgaaac caaggccaaa gtctcctaca
    33301 cctacaaggc ccgttacggc gcagcgctcg gttactacaa cttctcctcc aatgcagcat
    33361 tcgccggtta tcaggcaaac gcaggaacca cggcgaccga ttactttggt gatggcaccg
    33421 taaccgcctt cgtgccggaa atcttcttca ttccggtaca gaatgtccgc attggcctgc
    33481 agtacaccat gtacacgcag attccgaccc agacggccgc caaccagacc ttcacggggg
    33541 ccaacgccaa cggacagccg tggaactcca gcgatttcaa cgaggcattc ctgtatgtct
    33601 gggcggccta ctgatcgcgg cttgcaacgt cccaacggat ggacacgccc ggcgggcgtg
    33661 tccgaacctg aacaaagcag tggagaagtt caatgaaaaa gatcaccctg agcctggctc
    33721 tcgcgctgac cgcactggct agcggctgcg ccaacctgga acactcgcgc gatgttgcca
    33781 atcccgccgt aacgggcaag gtcatggccg aacagacctg ctcgatgtgc cacggtgtcg
    33841 acggcaattc gatcaacccg acgttcccca agctggccgg tcagcaaaag gaatacctgt
    33901 ctgacgagct gaagttcttt cgcagccaca accgcagtga cccggccggt tccgaataca
    33961 tgtggggcat cagccgccac ctgaccgacc agcagatcgc gcagctcgcc gactactatg
    34021 cggcgcagaa gcgcattccg caaccttcga ccaacgtacc gctggaagag ctgggcaaga
    34081 atatcttcct gaacggcatt caggatgggg cgcgcacggt tccggcctgc cagacctgtc
    34141 acggtgcgga tgcccatggc catggcgtct tcccttacat tgccggccag catgctgact
    34201 atatccgcaa gcagctcgac atcttcaagg aaaccgaaga acgcgacgaa aaagtgggcg
    34261 aaatgatgaa gaacgaaacc catgacctgt cgtccgagga aatgaaggcg gttgcggcct
    34321 acatcgccac catgccctga gccatattaa aagcaagctc ccggtaacaa ccccattcgc
    34381 gggcatcctc aggatgcccg ttttcttttt gcggtacaat gaccgctttc caacgcacga
    34441 cgcaaaccga tgagcatcca gtggttcccc gggcacatga ccgcggcacg caagaaagcg
    34501 gccgagacca tggcccgtac cgacgtggtg atcgaggtgg tcgacgcccg tctgcccgaa
    34561 gccagcacca acccgatgat cggcgaactg cgccggttcc gccagcgtcc gtgtctgaag
    34621 atcctcaaca aggccgatct ggccgacccg ctcgccacgc gcgcctggct cgaccgctac
    34681 gaccgtcagc ccggggtcac cgccgtggcg ctgtcatgca agacaccggc cgatgtggca
    34741 cgcatcccca aactgtgcct gaagctggcg cctggccgcg gcatccccag caaaccgctg
    34801 cgcatgatga tcatgggcat tcccaacgtc ggcaagtcca cgttgatgaa cgcactgctg
    34861 cgccgacgtg tggccgctgt cggagacgag ccggccgtga ccaagagcca gcagcggctc
    34921 gaactcaacg accatatggt actggtggac acgcccggca tgatgtggcc gcgcatcgaa
    34981 tttccgtccg atggcctgat gctggcagca agccatgcca tcggccgcaa tgcactgtac
    35041 gaagacgagg tagcgcagtt tctcgccgac gtattgcgcg aacgctaccc cgaattgctc
    35101 gcgcagcgct atggcatcaa tgtcgccaca ctggatgccc cggcaatcat cgacgccatc
    35161 gcgcgcaagc gcggctatca cgtgcgcggc ggcgaactcg atcaggaaaa ggcagccatg
    35221 acgctgctgc aggattaccg cagtggcgcg ctcggacgca tcagcctcga aacgcctgac
    35281 agccgcgcgc gcatggtcag cgatgcccga gctgcagcac aggcgatggc tgatgcagcc
    35341 gcagcggcag acgaaccgcc ggtggcgtca gccgactgac ggcggcaggc cgagccggcg
    35401 ccagatctcc agcgttgccg atgcctggtt catggtgtag atgtgcaggc cgggcgcgcc
    35461 atgatccagc agacgctggc acatggcggt caccagatcg aggccaaacg cacggatcga
    35521 atcgatgtca tcctgccagc cttcgagctt cttgcgcacc cagcgcggaa tttctgcccc
    35581 gcatgcgtcg gaaaaccgcg ccagctgggc aaaattgttg atcggcatga tgccgggcac
    35641 caccggcacg gaaataccgg ccttgccgca atcgtcgaca aaggcgaaat aggcatcggc
    35701 attgaaaaaa tactgcgtga tggccaggtt ggcgccggca tcgatcttgg tcttgaaatg
    35761 cagcagatcc tcagccgcgc tgcgtgcctg cgggtgcact tcgggatagg cggccacggc
    35821 aatgtcgaac caggcccccg tctcggcacg gatgaatgcc accagatcgc tggcataccg
    35881 gaattcaccg atgcctgcca tgcctgacgg caggtcgcca cgcaaggcga cgatatggcg
    35941 aataccgtgc tcacgatatt gcgccagcac ctcgcggatg ctgtcgcgcg tggcaccgat
    36001 acaggacagg tgcggtgcgg cggtcagacc ggctgcacga atgtccagca ccgccgccag
    36061 cgagggctcg cgcgtcgatc cccccgcacc ataggtcacg gagaagaacc gtggctccag
    36121 cacggccagt tggtcacgca cctcgcgcaa gcgttcaaaa ccttccgccg tcttgggcgg
    36181 aaaaaactcg aaactcgacc acatgcgact gccccccgtg accatcaata gcggtaatgt
    36241 gccggcttgt acggtccgtt gcgctccacg cccagatacg ccgcctgctg gtcggtcagt
    36301 tcggtcagtt cgacgttgag cgtcttgagc tggaggcggg ccactttctc gtcgagctgc
    36361 ttcggcagtg tatacacgcc caccgggtaa tgcccactgc gggtaaacaa ttcgatctgc
    36421 gccagcgtct ggttggcaaa cgacgaactc atgacatagg atggatggcc ggtggcgcaa
    36481 ccgagattca ccagccgccc gcgcgccagc aaaatgatgc gcttgccgtc agggaagatc
    36541 acatgatcga cctgcggctt gatctcttcc cacgggtagc gttcaagact ggcgacgtcg
    36601 atttcgttgt cgaagtggcc gatgttgcac acgatggcct ggtgtttcat gcgctgcatg
    36661 tgctcgtggg taatgacatg gtaattgccg gtagcggtga cgaaaatgtc cgccttgtct
    36721 gcggcgtagt ccatcgtcac cacacggtaa ccttccatgg ccgcctgcag cgcacagatg
    36781 ggatcgatct cggtgaccca cacctgcgca gacagcgcgc gcaaggcctg ggcagacccc
    36841 ttgcccacat cgccgaaacc gcacaccagc gcgatcttgc ccgccaccat cacgtcagtg
    36901 gcccgcttga tgccatcgac cagcgattcg cggcatccat aaaggttgtc gaacttggat
    36961 ttggtcaccg aatcgttgac attgatcgcc ggtacgcgca attcgccacg ctcatgcatc
    37021 tggtagagcc ggtgcacgcc ggtagtggtt tcctcggtca caccgcggat gtgcgacagc
    37081 cgggtcgaat accagcccgg ctggtccgcc agccgtttgc ggatcgcggc aaacagcgcg
    37141 cgctcttcct cgctggcctg attgtccagc accgaaggat cctgctcggc gcgactgccg
    37201 agatgcagca gcagcgtggc gtcgccgccg tcatcgagaa tcatgttgga aaaaccgcca
    37261 tccggccatt cgaaaatacg gtgggtgtat tcccagtagt cctccaccga ttcgcccttg
    37321 acggcaaaca ccggcgtgcc ggtggcggca atcgccgccg ccgcatgatc ctgggtggaa
    37381 tagatgttgc acgaggccca gcgcacgttc gcgcccagcg cctgcaatgt ttcgatcagc
    37441 acaccggtct ggatggtcat gtgcagactg ccggaaatgc gcgcgccctg cagcggccgg
    37501 gtggtggaat attcttcgcg gatggccatc aggccgggca tttcggtttc ggcaatcgcc
    37561 agttcgcggc gaccccagcc ggccagcgcc agatcagcaa ccacgtactc gggggaaggg
    37621 gaagatttgg cgtgcatcac gctctccttt cttgaatggc aaaaaaagac aaacgtgagc
    37681 gccgttgcac atccatggcc gagcctggca ggggaatccc cccgtcgcaa cgctcctcga
    37741 accagagacg cattctagcc aaagcaggca gaaaagtata tccaccagcc ggcgctttcg
    37801 tacctcgcac catgaccgcc gccgacaggc cgaatgagca tgctctgcga cgaccgctgt
    37861 ccgcaagccc ggtgcccctc cgttaccgcc agagcgcatg catcgcctga tccgccaccc
    37921 gaagctgcaa catcgtggat aacaggcgta cactgacctc cgggacaacc atgctgcaag
    37981 gagaacgaca tcatgttcga atgcaatcaa ggctggatgt atggcggcgg gtggatgctg
    38041 tttggctggc tatggatggt gttgctgatg ctggcacccg tactgctggt cgccgcgctg
    38101 gtcaaatacg tgtttggcgg gtggcaggtg aagacgcaga ccagacgtgc ggcgctggac
    38161 atcctgaatg aggtctatgc gcgcggcgga atgtcgcgtg aggaatacct gcaaaaacgc
    38221 gacgatctgc gggaaaagcc gtagcacacc cgttgcagcc cgcctcagcg aaccggtcag
    38281 acgggcatgc gcgcatgcgc gactttcgcc agcgcaatcg aatcgagacg cgcctgctcg
    38341 gaatcggcac gcgaggtcag caccaccgga acgcttgccc cgagcacgat gccggcaaag
    38401 cgtgcgccgg caaggtattc gagcgccttg tacaggacat tgcccgacac caggtcgggc
    38461 atgagcagaa tgtcggcccg gccggcaacc gggctgacga tgcccttgat ggccgctgct
    38521 tcggcagaaa tggcgttgtc gaacgccagc gggccatcga cgtgcgcatc gccgaagtgg
    38581 ccgcgatcgg ccattttcgc cagcgcagcc gcatcaagcg tggaaacgat ggcgggattg
    38641 acatcctcca ccgccgacag caccgccaca ttcggccgac gttcacccag cgcgcgggcc
    38701 agcgccagcg cgttgtgcag aatgtcagcc ttggccagca gatccggcgc aatgttgatg
    38761 gcggcatcgg taatgagcag caatctggga tagcgggcaa gttcggtcac gaatacgtgc
    38821 gaaatgcgca ccgtaccgtg cagttccctg acaatgggat gaagaaactc cgccgtatgc
    38881 aaatgcccct tgatcagcat gtgcacccgg ccatgcttga cttcctgcac ggccagcagc
    38941 gctgcatgcg catcatcttc ggcttcgagc accggatgac tgcaaccgtg ttcgagacac
    39001 aacggctcga tctcgtcacg acgaccgatc agcagcggtt cgatcaggcc ctcgcgcgcg
    39061 gcgtgcagtg ccgcatcgag cacgtgccgc tcgccggccg cgaccacggc ggcaggcacc
    39121 ggggccagcg cgcgcgcctc ggcctgcaat ccgtcaaagc tgcggatcga cgtaaacatg
    39181 ggcttaccct gcggcggcga actcgtcgag cgcatgccgg agcggagtca ggtacatcag
    39241 cgcggggccg ccatgcatca tcaccgccat gaatccggcg ctcatgacct catcgcgggt
    39301 cgcgccggcc ttgagcgcct gctgcacatg cagcgcaatg caccattcgc actgggcgct
    39361 gatggcgagc gcgacgccga tcagttcctt ggtgcgcagg tcgagggcct tgccgccctc
    39421 ggctttctgc atgaactgaa ggaaagaacc ggtttcttcg ggatattcgg cgtgcaactg
    39481 cttgagcagt gtatcgattt cggccagctt ttcagtcatg gacatgattg catctccttg
    39541 aacagaacag ggacttgcgc cagccagcat gacacgaaac cacaggcggc tcaagcgccg
    39601 cccgccgaat ggacagtccg tcagtccaga ccggcatcgg cgcgcagcgc ctccgccttg
    39661 tcagtggcct cccaggtgaa ttccggctca tcacggccga aatgcccgta gcttgccgtt
    39721 cgggtataga tcggccgtaa caggcgcaga tgctggatga tggccttggg acgcagatcg
    39781 aaatgctgcg cgatgagctc cgtgatgcgc tcgtcgctga tgcgaccggt gccgaaggta
    39841 ttgaccatca gcgacaccgg acgggccacg ccgatcgcat aagccacctg caccagacag
    39901 cggcgcgcca gaccggcagc caccacgttc ttggccacat aacggccagc gtaggccgcc
    39961 gaacggtcga ccttgctcgg atccttgccg gaaaaggcgc caccaccgtg cggtgcggcc
    40021 ccgccgtagg tgtcaacgat gatcttgcgc ccggtcaggc cggcatcgcc catcgggcca
    40081 ccaatgacga aacggcctgt cggattgacc aggtaacgca ccgcaccttc cagcagttgc
    40141 ggcgcaatcg ccggtctgat gatctcctcg atcaccgctt cctcgatctg cgcatgcgaa
    40201 acgtcgggat ggtgctgggt ggagatcacc acagtatcca cgtgcttcgg cacgccattt
    40261 tcataacgca ccgacacctg ggatttggca tcgggccgca gccaggccag acgcccatcc
    40321 cgccgcagct gcgattgccg ctccatgagg cggtgcgcaa ggtagatcgg cagcggcatc
    40381 agcgcggggg tctcgtcgca ggcataaccg aacatcaagc cctggtcgcc ggcgccctgg
    40441 tcgagatcca gcccttcacc ctcattcact ccgcgagcaa tgtcgacgga ctgtttgcca
    40501 aacgtggtca gtacggcaca ggtgcggtga tcgaagccaa tctcggagct gtcgtaaccg
    40561 atgcgctgga tggtggcgcg cgcgatctgg ttgtagtcga tcatggcatg ggtggtgatc
    40621 tcgccggcaa tcacgaccag tccggtggtc accagcgtct cgcaagctac ccgggcatgg
    40681 gcatcctggg ttagaatagc gtcgagcacg gcgtcagaaa tctggtcggc gaccttgtcc
    40741 gggtgtcctt ccgacaccga ttccgatgta aaaatatatt ccgtcatcat gcttgttgtc
    40801 cgctttccga ggctatgcac ataacaactg gtcatgctat cacaagtccg gctcacggat
    40861 gaactatgcg atgaccctga ttctgcgact gctggcccgt cttcccctgc gtttctggca
    40921 cgctgtcggc gcctgggcag gcacacaggt cttcctgcgc aaccggcgtt acgccgcgcg
    40981 catgcgcgac aatctgcgcc agtcgggact cgctgccgat gcgccggcgc tcgatgcact
    41041 ggtgcgcgcc aacgcgcgcg aactgggcaa ggctgcggcg gagatcatcc cgatctggtt
    41101 ccgtcctttt ccgcaagtgc tcgacctggt ggtcgaatgc cgtggctggg agcacgtcga
    41161 gcccgcacgc gccagtggac acggcattct tgccatgacg ccgcacctgg gctgcttcga
    41221 gatc
//
LOCUS       DAIANL010000046        11518 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_46773, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000046 DAIANL010000000
VERSION     DAIANL010000046.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11518)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11518
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46773"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2376 a         3746 c         3253 g         2143 t
ORIGIN      
        1 gtcgtggacg ggcgcgccgg tatttccctg aaccagatca ggcactgccg cttctcgcaa
       61 ctaggtaact gatcatgctc aacgtgaagt agccgccacc ctcgggcatg aagaacacga
      121 tgatcgagaa gaacatcagg aaaacgacta ctccgacgat atccttcacc gtgtagtacg
      181 gatggaaagg aatgccgtcc agcggaatgt gggtcaccgg atccttcacc ttcttgatct
      241 cgataccttc ggggttgttg gagcccactt cgtgcagcgc aatgatgtgt gccaccacca
      301 ggccaagcag caccagcggc agggcgatca cgtggaacgc aaagaagcgg ttcagggttg
      361 catcggacac cacgaagtca ccgcggatcc aggtgctgag cgcatcgccc accacaggaa
      421 tcgcgctgaa cagcgagatg atgacctgtg cgccccagaa cgacatctga ccccacggca
      481 gcagatagcc catgaaggct tcggccatca gcgccagata gatcatcatg ccgaagatcc
      541 acaccagttc gcgcggcttg cggaacgagc cataaatcag gccgcggaac atgtgcaggt
      601 agaccaccac aaagaacatc gaggcgccga ccgaatgcat gtagcggatc agccagcccc
      661 actgcacatc acgcatgatg tactcgaccg acgcaaacgc ctcggtcgca gcaggcttgt
      721 agttcatggt aaggaagatg ccggtcacga tctggttgac gagcaccagc agcgcgaggg
      781 agccgaagaa ataccagaaa ttgaaattct tcggggcata gtattcggac aggtgagctt
      841 tccacgtaga cgtcagcggg aaacgctcat ccacccagcc cagcaatttt tgcatcgcag
      901 ccatgaatca agctcctttc ttttcttcac cgaccagaac acgcttctcg gtcatgtaat
      961 aatgcggagg caccagcatg ttcatcggcg cgggaacatt ctttaatacg cgcgcggaca
     1021 ggtcgtagcg cgatccgtgg cacgggcaga accagccacc gggccagtcg gcgccgagat
     1081 cgggaggccc gagttccggg cgataggtgg gcgagcagcc gaggtgggta cagataccca
     1141 cgaccacgag atactcgggc ttgatcgaac ggtagatgtt cttgcagttc tccggctgct
     1201 ggggaacgtc gctgttgggg tcaaccagac ggtcagcaag accgggcaac ttgtcgagca
     1261 tcgccttggt gcggttcacc acccagaccg gcttgccttg ccaggccacc gcaagctgca
     1321 tgccaggctc gaccttgctg atatcgacct cgacaggcgc accggcagcc tttgcccgct
     1381 cactgggcag catgctcatc acgaatggcg tcaacgccac caccccggcc gcacccccca
     1441 ccactgacgt ggccgcgacc agaaagcggc gcttgctgcg atccacttgc tgattcatca
     1501 tataatcaat ccccattcag gttacgcgac ttaaaaacct tattatttta cctaacattt
     1561 caacaaaaca aaaagctaaa ttttcgtatt gctgacctgt tacattgtta atgcgaacag
     1621 catccgcgcg gcctgaccgg cattcatcgt ccgcatcagg tataatggcc gcatcaattc
     1681 cggccggatt gcgaaggatg gcggttggcc aaatcgcccc ctgacattac gcattgtgcg
     1741 tcactgctgg cggacaatgt ggatcaaacc atgcgaaaat tctggctgct gttcgctcaa
     1801 agcacaacca ttgcgctcgg attcctgttg gcgctcaccc tgtttcgccc cgactggcgc
     1861 atgtggccag acactgcgca cagcccccct gccatcacct gccagccggc aaactgcgcc
     1921 accgattcat accgcgatgc ggcgcgcaag gccatcccat cagtagtaaa cgtattcacc
     1981 agcaaggcga tccactcgcc gcgaaccctg ccagacagcc cgttcttccg ccatttcttc
     2041 ggcaaacaga atgaaccgcg cgtacgccgc agcaacaacc tcggttcagg cgtcatcgtc
     2101 agtgcagacg gttacatcct caccaaccac cacgtcgtcg aatccgcaga cgaaatccag
     2161 gtggccctgc atgacggccg catcctgcag gctcgtgtcg tcggcagcga tcccgaatcc
     2221 gatctcgccg tgctcaagat ccaggcaaag gggctccctg caatcacctt cgcccagtcc
     2281 gacgatgcca gtatcggcga tgtggtactg gcgatcggcg accccttcgg tgtcggccag
     2341 acggtcacca tgggcatcat cagcgcactg aaacgctcgc acctgggtat caatatcttc
     2401 gaggacttca tccagaccga tgcggcgatc aatcccggca actcgggcgg cgccctggtc
     2461 gacacgcatg gcaacctggt cggcatcaac agcgccatct actcgcgcac gggtggatca
     2521 cagggtatcg gtttcgccat cccgtccaac cttgccgcag aagtcatgcg ccagatcatc
     2581 gcgcacggcg aagtcatacg cggctggatc ggtgtcgaag tgcaggacct gtcgccggaa
     2641 ctggcgcaaa gctggaaact cgccagcagc aatggcgctc tcatcgcggg tgtactgcgc
     2701 agcggcccgg cagaccgtgc cggcatccag cccggcgaca tcgtactgca agtcaaccat
     2761 cgcccggtcg aggactcgtc cgacctgctc aacgacatcg ctggactgac gcctggccag
     2821 acggtaccga tcgaagtcgt gcaccgctcg cagacacgcg aaatgatggt gcgcatcgcc
     2881 cgccgcccga agatccggca ctgacgggca acacgcgccg cttacagcga attgcgccat
     2941 tcctgatctt cgccgtcgaa caggcggttg gcttcgagcg gcccccacgt tcccgcagag
     3001 taggtatgaa tgaaatcccg gtcctgcacc cagctcttga ggatcgggtc gactacgcgc
     3061 caggcccact cgacctcgtc gaaacggatg aacagcgtgc gatcgccgtg caccacatcc
     3121 agcagcagcg cctcataggc atccagcggt ttttcgtcat ccttgcgata actcgcattg
     3181 agcgagatca cgcgcgtatc catgcccagt cctggctgtt tgacgtgcac ttcgatgcgc
     3241 atactgtctt ccggctgaat ggacaggatg atccagttgg gtgacagctc ctcaagtggc
     3301 gttgagcgga acaattgttg cggcgggtga cgaaagcgca gcgccaccat cgagcgggct
     3361 tccttgagcc gctttccggt gcgcagatag aacggcacac cgcgccagcg ccagttgtcg
     3421 acgtaaaact tggcggcgac gaaggtttcg gtgatcgatt tcggctcgac gcccggttcg
     3481 gcctgatagc ctggcacatt ctcgccgcca atggtaccgg ccgcatactg cgcgcgaaat
     3541 gcatgcgcat gcactgaacg cttgctgatc gggcggatcg aacgcaacac tttgaccttc
     3601 tcgtcacgca gcgcgtccgc atcgaacacc ggcggcggct ccatcgccac caccgtcagc
     3661 agctgcatca ggtgattctg cagcatgtcg cgcattgcgc cggacttgtc gtagtaatcg
     3721 gcccggcctt cgatgcccag cgtctcggca gcggtaatct ggatgtgatc gatgaagttc
     3781 cggttccaca gcggctcgat cagggtattg gcaaagcgga acaccagcag gttctgtacc
     3841 gtgtccttgc cgaggtaatg gtcgatgcgg aaaatctggt cctcgtcaaa atattcatgc
     3901 aacagccggt tcagcgattg cgccgactcg atatcctcgc cgaacggttt ttccaccacc
     3961 acccggtgct ggccacgcgg cttcgacaga cctgactcat ccagattctt gatgacgctg
     4021 gaaaaatcgg tcggcttgat cgacaggtag aaaatcacgt tggtgcacac gcccatgcgt
     4081 ggccggctca gatccgcacc cagctgacgg tacatggcca ggtcatgcag ctcgccgcgc
     4141 agataggtga accgcgcgca aaaacgttcg caaacgacct cgtcataacg ctcctgcagc
     4201 ttttcgcgca gcagggcccg catgtggtca cgccatgcct catcgtccca ctcgcgacgg
     4261 ccgatggcga tgaataccat gtcctgcggc aaccgaccag cttcctcgag ccggcacagt
     4321 gccggaatca gctttttact tgcaaggttt ccggtcgcgc caaaaatgac aaagctgcat
     4381 ggaaacatcg attcatcatt catgcgcacc cccttttact gcgtgcccgc caaacccctt
     4441 gcgcatcatc gccagcaatc tgtcagtgta ttcgttgcga ccctggctgg cgaagcgcat
     4501 catcagtgac agactcatca ccggggtcgg cacgccaagt tctatcgact ccagcgccgt
     4561 ccagcgcccc tcacctgaat ctgccacaaa cggttcgatc gattgcaacg tctgatcctg
     4621 tttcaggaag tccgcggtca ggtcgagcaa ccaggaacgc accacactgc catgacgcca
     4681 catctcgctg atggcagcga gatcaagggc gaaatcctcg cgcgcacgca tgagggccaa
     4741 tccttccgcc agcgcctgca tcatgccgta ctcgatgccg ttatgcacca tcttgacata
     4801 atgtccgctg cctgccggtc cgcaatgcag ccagccggca tcgggcgccg gtgccagcaa
     4861 ccgggcatac ggctcgaccc gggatacggc ttcgcgcgtg ccgcccagca tcagcgcgta
     4921 accgttgtcc agtccccaca caccgccgga cacgccggca tcgacgaact gcagaccatg
     4981 tcgcgccagt tcttcggcat ggcgctggct gtccctgaac caggcattgg cgccgttgac
     5041 caccacgtca cccgccgaca ggtgcccggc cagttcctgc atcactgctt cggtaggtgc
     5101 tccggccggc aacatgagcc agaccagacg cggcgcgggc aaactcgcca ccagctggcc
     5161 gatcgaagca tgcgccccga ttcccggctg cccggcagcc agttgttccg ctacgtcaag
     5221 gctacgattc cacaccgata cgctcacacc cgcacgcgcc aggcgtcgag ccatgttcgc
     5281 acccattctg cccaatccca gcaaaccgat ctgcatgctg cctcctggtt atcaattgcg
     5341 cgcgccgcca cccggccgta cgctgcccgg caaattaacc acagataaat aacaaatgca
     5401 atcgggcaaa ccgatctcgg caataccgcc ccacccgccg ttgcattgcc agcaatgcac
     5461 cagcgcggtg catgtcaggc tggcgccggc cagaattcag gcaaacgcca gctgaaaatc
     5521 cgtcagacag tcccgtcctg cccggcagga agacccgcca ggcgtgcgtc tgcacgtcct
     5581 ggcaccgcca cgacctgtcc ttcatgattc aggcatgttt actgctagag ttcggctgac
     5641 gtgaatccac acaagggaga acggccatga gtgaaaaaat gatccgcatc gcaaccgcag
     5701 gcaccattgt ctctctgatc gccagcgctt tgcttgcaac catccatccg gacacgaaac
     5761 gcatggaaca tgagatcgtg gcgggagaca tgcacgcgct ggggcagatg caggccttga
     5821 tggacgaggg caatgccgtc gcacagaact ggctcggcct gcatttccag tccggctcgg
     5881 attatgtcag cgcagcccgc tggttcctca aggcggccga ccagggcaat gtgctggcgc
     5941 aggacaacct cggcaacctc tacgaaaccg gccgcggtgt cctgcccaat ccggttgtgg
     6001 cctacgcgct ctacagcctg gcggcatcaa gcaaccagcg ctgggttcgc ctgcattcgc
     6061 gcacatcgct gcagcgggta ggcgcgtcca tgagcgatga gcagctcgac gccgcccgtg
     6121 caatcgcaca gatgatgcga agcgacggga tcgccagcac cctcgatcgc atcgaacaca
     6181 tccaggacgg ctacgagccc tgacgccgac ccgagccaca gacctgcctg acgccagcgg
     6241 gcaggtcggg tccccagcgc cgggcatcgt cccgccgggc cagccaaaac cgcccgaccc
     6301 gcggtctagg ctgcgggtgg gaccgtgctg gcgaaggcgg gccgctcgcc cagtcgcgcc
     6361 gcaagtgcgg ccaggttggg gtaagagtcg cgccagcgga tatgcgaaaa acgcagatca
     6421 agatagccca gcgcgcagcc cagggaaatg tcagccaggg tatagctgtc gcccatacac
     6481 cagggatgat cgcccaggtc cctggcggca gcggccagac cgcggaaaat cttctgttcc
     6541 tggcgcaaca gccattccgg gctttgctgc ccgcccggcc gcttctgttc cagatagatc
     6601 gtggccgctg catcgcagat gccatcgccc agcgcttccc agcgcttgac aagcaaccgg
     6661 ccgcgcacgt ccttgggcaa cacgttgtgt accggcgaag cctgatcgag atattcgacg
     6721 atgacccgcg agtcgtagac cggtgtgccg tcgtcaagca gcagtaccgg gatcttgccc
     6781 agcggattga agtcgggaac gtgcgaatcg gcctcccacg gaatttcgag ctggaattcg
     6841 aaatcgatgt gtttttccgc cagcacgacc cggactttgc gggcatacgg actcgtcagg
     6901 gaagcgatca gtttcatggt tcagtccaca tgtgtttgaa tataattctt gagctggcca
     6961 gcatcggcgg gcagcgtcgc gtagcgctgc ggcaacgcct cgatcccttc cagcgcctgc
     7021 ggacgcggcg gcatctgccc aagcgcctcg cgcagcgtgt catcaaactt ggccggttgt
     7081 gcggtttcga gcacgatcat ggggataccc ggttcagtgt accccaaccc gactgccaga
     7141 ccatccgccg tgtgcggatc gatcatgatg cggtattttt cccaggtggt gcgaatcgtc
     7201 gcaatgcgcc ccgcgtgcgt gttcgatccc gacaccaggc cataatccgc cacacggtca
     7261 aacaccccgc cagaacggaa atcggccgcg ccaccctgtt cgatctgttg ccacagcgca
     7321 cgcagccgtt cgctgtcgcg gccaagcagg tcggccgcaa aacgctcgaa gtttgaagcc
     7381 ttggatatgt ccatcgacgg actgctggtc tcccgcgttt gcgcagccgg ccgcggccgg
     7441 tacaccccgg tggagaaaaa ttcgtcgagc acgtcgtttt cattggtggc aaccacgaga
     7501 cggcgaatcg gcaaccccat catgcgggca atatgcccgg cgcaaacatt gccgaaattg
     7561 cctgatggca ccgcaaagct cacctgttgc cgctcgtcgt gcgtggcgga aaaatacgcc
     7621 ttgaaatagt agatcacctg cgcggcaatg cgcccccagt tgatggaatt gaccgcaccg
     7681 atgtggtact gctgcttgaa tccggcatca gcggacaacg ccttgaccat gtcctggcaa
     7741 tcatcgaaca cgccttccac ggcaatattg aaaatattgc cgtcctgcag cgaatacatc
     7801 tgtgcgcgct gaaagcggct catcttgccg gccggtgaca acatgaacac gcggatgccg
     7861 cgctttccgc gcatggcgta ttctgccgct gagcccgtgt cccccgatgt tgcacccacg
     7921 atattgagcg tacgaccggt gcgttccagc acatactcga acaggttgcc gagcagctgc
     7981 atggccatgt ccttgaacgc cagcgtcggt ccattggaca gaccgagcag atgcagttcc
     8041 ggcgagagtt cgtgcagcgg tgtgatctcg cgcgcatcgt cacccgcacg cgtgtaggcg
     8101 aacgcgtcgg cacgataggt gcggtcaatc agctcgcgca gcgcctgtgc agggatatcg
     8161 tcggcaaagc gcgacaacac ggcaaatgcc agctggcgat aatccatgcc gcgcatcgcg
     8221 gcaaggtcag cggcttcgaa atgcgggtag cgttcgggaa ggtacaagcc tccatcaggc
     8281 gccagcccgt ccagcagtat ctgactgaac gtccgcgccg gagaatgtcc gcgagtgctc
     8341 acatattgca tggccatcat ctgtctcccg ccgatcagcc agccagatgt tcgagacgga
     8401 tacgcatcac gccacccgtc agcgacggca gcgcctccat gcgatcgatg gcggcatcga
     8461 tgtgcttttc gctgcactgg tgcgtcaaca tgacgatatc caccagcgga tcgcccgacg
     8521 ccggctcctg ctggatcatg gcttcgatcg aaatacccag atccgcaaga atgcgcgtga
     8581 catcggccag tacgccggcc tcgtcgcgcg ctcgcatccg caggtaatag caagtgcgca
     8641 cttcgcccat gggcaggatg ggctcgctcg acagggtttc cggctggaac gccaggtgcg
     8701 gcacgcgact gtgcgggtcg gatgtcatca tccgggcgac atcgaccaga tcggccacta
     8761 ccgacgaggc cgtcggttcg gcgcccgcac cggcaccgta gtgcagggtc acgccgaccg
     8821 cgtcgccctt gacgagaatg gcattcatcg acccgtcgac attggcgatc agccgccgtt
     8881 ccggaatcag tgccggatgc acgcgcagtt cgatgccgcc ggcggtgcgc cgggtgatgc
     8941 cgagcagttt gacccggtag ccaagccggc cggcgtaacg cacatcatcc tgtgtgaggc
     9001 gggtgatccc ttcgatatgc gcatgatcga actgcatggg aatgccgaaa gcaatggccg
     9061 agagaatggt cagtttgtgt gccgcgtcca cgccttccac gtcgaagctc gggtccgcct
     9121 cggcataacc cagttcctgg gccagcttca acgcatcggc gaaactggat ccttcgtcac
     9181 gcatgcgtgt aaggatgaag ttggtcgtgc cattgatgat gccagcgatc cattcgatgc
     9241 ggttggcggt caatccctcc cgaatcgcct tgatgatcgg aatgccgccg ccaacggcag
     9301 cctcgaatgc caccatgacg ccgcgctgct gcgcggctgc aaaaatttcc gtaccgtggc
     9361 gggcgatcag cgccttgttg gccgtgacca cgtgcttgcc gttggcgatg gcctccagga
     9421 tcagcgcacg tgcgggcgat tcaccgccca tggcctcgac cacgatgtcg atgtccgggt
     9481 ggttgaccac cgcaaacgga tcatcggtca gctgcatgtc gtcgccagca tgcgcctgcg
     9541 cgcgttgcag gtcacgcacc gccgccatga cgacgcggat ttcacatccg gcacgtcgct
     9601 tgatttcggc agcattgcgc cgcagcacgg tcagcgtgcc tccacctacc gtgcccagcc
     9661 ccagcaggcc cacttgtatc ggtttcattg tatgtgattc caccctgaca tggcgctcag
     9721 gcgccatgtg aattgcgata acgttccagg aagcgcgcga tgcgaccgat ggcctcgcgc
     9781 agttcgtcct cgtgcggcaa tattaccacg cggaaatggt ccggccacgg ccagttgaag
     9841 ccgctgccct gcaccaccag cacacgctct tcttcgagca gttgcaggat gaagcgctga
     9901 tcgtcctcga tcggatacaa cttcggatcc agccgcggga acatgtacag cgccgcctgc
     9961 ggcttcacgc aggtcactcc gggaatggcg gtcagcaatt cccaggcgag gtcgcgctgc
    10021 cgcgtcagtc gtccgcgagg cgccaccagt tcggtaatgc tctggtagcc acccagtgcg
    10081 gtctggatgg catactgtcc cggcacgtta gcgcacagac gcatcgaagc cagcatgttg
    10141 atgccctcga tataatcctg cgcatgacgc ttgtcgccgg aaatggccag ccagccggca
    10201 cggtagccac aggcgcggta gttcttcgat agtccgttca tggtgatgca cagcacatcc
    10261 tgcgccagcg aggcgatcga ggtgtgcgtg ttgccgtcgt agagcacctt gtcgtaaatt
    10321 tcgtcggcaa acacgatcaa ctggtactcg cgggcaatct gcaggatacc ttcgagcacc
    10381 tcccgaccat acagcgcacc ggtcgggttg ttcggattga tcaccacgat ggcacgggta
    10441 ttcggcgtca ccctggcgcg catatcggcc aggtcaggca tccagttcgc attctcgtca
    10501 cacaggtaat gcctgggcgt accaccggcc agcgtcaccg ctgccgtcca taatggataa
    10561 tccggcgcag gcaccagcac ttcatcgccg ttgtcgagca gcgcctgcat cgccagcacg
    10621 atcagttcag acacgccgtt gccgatgatg atgtcgtcga cctgcacgcc gacgatcccc
    10681 ttgctctgag tgtagtgcat gatcgccttg cgtgcggcga acagaccacg cgaatcactg
    10741 tagccggacg attccggcag attgacgatc acatcacgca cgatctcgtc cggcgcggta
    10801 aacccgaacg gcgccggatt gccgatattg agcttgatga tgcgctggcc ttcatcttcc
    10861 atctgtcgcg cacgcgccag caccggacca cgtatgtcat agcagacgtt gtcgagcttg
    10921 ctcgatttcc cgacctttcg cgtcaccggt tttgcttccg tcaccacaca cccatcccga
    10981 ccatctcccc agttgggaga acgcgtctgc aaaaggtata atcttactcg actgtgcacc
    11041 tgcgccgcaa ttggatacca tcgtgaaatt tcacctgaat caggcagaag gcctgaacct
    11101 gtttaccggt tatggcgacg gccacgtatt cgtcaaccag caacgtcatg acgagacaag
    11161 cctggtcgtt actcccgaag acatcctgcg cgactggtgc gacgcaggct acgaacagtt
    11221 gtcgcctgcc catttcgcca tcctgcgaga cttgcatccg gaagtggcgc tgcttggcac
    11281 cggaacgcag cagcgctttc cgcatccgcg cctgaccgca cccctgatcg atgccggaat
    11341 cggcctggaa gtcatggaca cgcgcgcagc ctgccgcacc tacaacatcc tcgccgcaga
    11401 aggccgtcgc gtggcaatcg ccctgctgtg gcgctgaacc gttgccagtc acgccctggt
    11461 tttcagcagc acgggacgcc gtggacggat tccgccacga accgcatcac cgatcggt
//
LOCUS       DAIANL010000047         4239 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_455400, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000047 DAIANL010000000
VERSION     DAIANL010000047.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455400"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          874 a         1088 c         1473 g          804 t
ORIGIN      
        1 tgaactggac agcatgctgg aaatgcagcg ccagggagac gcaacggaac atgcgccgat
       61 cggtgaggtg ctggtggagc agaaagtggt ccaggaaccg gtggtcgaag cggcgctggt
      121 gcggcagaag cagatcaagg aaaaccgcaa cgcgcagaat gcctcgattc gtgtggattc
      181 cgcccggctc gaccagctca tcaacctggt cggtgagctc atcatcgttg gatcgagcgc
      241 caatctgatc gcacgccaga ttgcgcatcc cgctttgctg gaagtcactt acaacatgac
      301 ccgcatggtg gaagaagtgc gggattcggc aatgtcgctg cgcatggtgc agatcggcgc
      361 gacattcagc cgttttcagc gggtggtgca tgatgtggcg caggaactgg gcaaagatat
      421 tcgtctggaa atttccgggg gtgaaaccga gctggacaag acggtggtgg aaaaaatcac
      481 cgatccgctg acgcatctgg tgcgcaatgc catggatcat ggcattgaat cgcgtgcgca
      541 gcgcgcggca aatggcaaac ccgaacaggg tgtgctgcgt ctcaatgcgt ggcacgagtc
      601 gggcagcatc gtcatcgagg tggctgatga cggtggcgga ctcaatacgg aaaagattcg
      661 tgccaaggcc atcgaacgcg gattgatcgc agccgatgcc ggtttgagcg aacacgatat
      721 tcatcagctg atctttgccg ccggattttc caccgcagat gcggtcagca atctgtccgg
      781 acgtggcgtg ggcatggatg tggtgcgcag caacatcgaa tccctgcgcg gtagcgtcga
      841 actgttgagc caggcgggtg ccggaacggc ggtacgcatc catttgccac tgacgctggc
      901 gatcatcgac ggctttctcg tgggggtggg ccgggagcgt tatgtcattc cgctggagac
      961 agtggaggaa tgtatccagt tttccgtgac cgagctgaat ctggcgtctg gcgaggcagg
     1021 gtatatggat ttgcgtggcg aggtgttgcc gtgtgtgcgt ctgcgcgatg tgttcgatgt
     1081 gcgtcacagc agtgcgacgc gcgaaagcat tgtggtggta cggcatggtg cgcgccgtgt
     1141 cgggctggtg gtcaatgcgc tctatggcga actgcaggcc gtgatcaagc cgctgggccg
     1201 actgttcgag gcggctcagg gggtcggggg cttcaccatc ctcggggatg gcgaggtgac
     1261 cccggtgatc gacgtggcgc gcttgatcga gctttcggcc ggaccgctgc attcccgggc
     1321 aggcggcctg cccgcagcgg catgaaccgg atgacaggat gggctggtga tgggaaggca
     1381 ataaaaacgc ggcggtgtca tggcctggat gctgtggcga ctgctggagg caatcggaat
     1441 ttttatctga ctagtttggg agaatgacca tgttcaacaa tctgaaaatc ggcgtcagga
     1501 tgggtctggc gtttgtcatc ctgctggggc tgatgctgtt gctcggtaat gaggggctgt
     1561 cgcagctggg caacatgaac aaatcgatgc aggacatcat gcagttgcgt tatccgcaaa
     1621 gcagcgaggc caacaagatc gccatcactg tggaagaaat gggacgtgcg gcgcgtgata
     1681 tggcgctgac tgactcggcc gaagacatcc gtcgccagat cgacatcatc cagactgatc
     1741 ggcagcacaa taccgaactg ctggacaagc tggagaaatc gctggtcacc gaagaaggca
     1801 agcggttgtt cgataccgtg catcagacgc gagagaagta taaggaagct caggaccagt
     1861 atgtcggcct gatccagagc ggcaagcatg atcaggcggt gacgttcctg ctcagcgatg
     1921 tgcgcccggc tcaggaaggc tacctgaagg ccatcaatga caatatcaag ttttatgagg
     1981 acggcatgga tcaggcggag gccgtggcgc aggaagacta ccagtcggcc cgccacagca
     2041 cgatcgtgat cggtgcggtg gcgctggcgc tcggcatcgg actggccctg tggatcacgc
     2101 tgtcgatcac ccgcccgatc aatcaggctg taactgccgc gaaccgactg gcggaaggtg
     2161 acctgaccgt gcgtctgaac gtgagttcgc gtgatgaggt cgggcagatg atgcgggcca
     2221 tgcagaacat ggtggaaaag atttccgcca tcatcaccga agtgcgcagc gcggccgaca
     2281 atctgtcggc ggcttccgaa caggtgtctg cgaccgcgca gtcgctgtcg caggcgtcgt
     2341 ccgaacaggc cgccagtgtg gaggaaacgt cggcgtcggt ggagcagatg tcggcatcga
     2401 tcaaccagaa cacggaaaat tccaaggtga ccgatggcat ggcgaccact gcggccagac
     2461 aggcagtcga gggcggcaag gcggtggtgg aaacggttgc ggccatgaaa cagatcgccg
     2521 acaagatcgg catcatcgac gacattgcct atcagacgaa cctgctggcc ctgaatgcgg
     2581 cgatcgaggc ggcgcgtgcg ggcgagcacg gcaaggggtt cgcggtggtg gctgccgaag
     2641 tgcgcaagct ggctgaacgc agccaggtcg cagcgcagga gatcggtgaa accgccaagg
     2701 gtagcgttgg tctggccagc cgtgccgggg aactgctcga tgagatcgta ccagccatca
     2761 cgcggacctc cgatctggtg caggaaatca gtgctgcgtc gcaggaacag tctgcaggcg
     2821 tgtcacagat caatatggcg atgggtcagc tcaattcggt gacccagcag aatgcatcca
     2881 gcagtgaaga gctggcggcg acgtccgagg aaatgagtgg acaggccgag cagttgcagc
     2941 aattgatgca gttcttccgt ctcaacgagg aaacatcgcg tgcgtcgatg ccgacaatgc
     3001 accgtgcgcc ccagtccgga gtgaaggctg tgcgcgccaa accggtgctt gcagcccaga
     3061 gcgaagctcc tgagtccggc ttcgttgcat tctaggaggc ggccatggga gcattggtca
     3121 aaatggacaa tgccgcgacg gaggtccgga cgcggcagga gccggaacag tatctgacgt
     3181 tcctgctcgg gggcgaaatg tttgccatcg gtatcctggt gatcaaggaa atcatcgaat
     3241 tcggccaggt gacggaagtg ccgcgtatgc ctggttgcgt tcgcggggtg atcaatctgc
     3301 gcggtgctgt ggtgccggtc atcgatctct ccgtgcgttt cggtcgtccg gtgaccgagc
     3361 caggacgtcg cacctgcatc gtgatcgtcg aaactggcga aggcgaagcg cggcaggtga
     3421 tcggggtcat ggtcgatgcg gtgagcgagg tgctggaaat cccgcaggcc gatatcgaac
     3481 cggcgccagg gtttggcacc cgtatccgcg cggatttcat tgccgggatg ggcaaggtgc
     3541 gcgggcggtt cgtcatgata ctcaatgtcg agcaagtatt cctgatcgac gagatggccg
     3601 gactggacac ggccgagaca caggttgcgc attgagggca ccggttcagg cgtctgactg
     3661 gatgggtggg cacaggattg ggtggttgtg gtgaacgaac cggtaagctg gatgaaaatg
     3721 aacttctgcg cctcggcagg ggcgatttgt cagggagcgg acgcaatgca tttcattgac
     3781 tgggtcggca tgctgtcggt aagaaccggt gtgcctgcgg cactgcttgc ggtgtttacg
     3841 gagccggcat gatggtggtg cgctccggtc cgggcatgca acccggcgac gaatggttgc
     3901 gcagcctggc cgagatcccg ggcggtccgg tgatcctgct gcacgacatg aatgatgccg
     3961 gacgtattgt gtatgccaac gaagcggctt gccggcattt cggcgtggat cgttcgacgc
     4021 tgctgggctg gcgaccacag gactacgatc cgtcgtcatc ggcggccgaa gtcggcgcag
     4081 cggacggcca ggcggttcgt gcaggcgggc accgttttga aaccctgcac cgtcgcgcca
     4141 acggtgaact ggtgccggtg gaggtgctgc tggcacatgt agacggtgcg cagggtacat
     4201 ggctggtcag catggtattc gatatcagcg agcgcaagc
//
LOCUS       DAIANL010000048        22130 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2087520, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000048 DAIANL010000000
VERSION     DAIANL010000048.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 22130)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22130)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22130
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087520"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4562 a         6571 c         6622 g         4375 t
ORIGIN      
        1 atgagtctgt ttctgtcgca agcgtattgg tcagggcttc aatcatgctg caatcgaaag
       61 tcggaatacc gagcgtggca tgcacgcgct gacgttcgga gaagctgtca tcaacataaa
      121 tggccccttc gtgtcggatg aaacggcttt tgcattcccc gtccgtaaca tgaatcacct
      181 catcgaatac tccacccaga tgatgccggt tcagcgttgc ggtcagatct ccggcatgcc
      241 gggtaatcag gattaccgga accccgcggt taatgcagga aaaaacgagt ttcagtgcat
      301 cgagattgat ccggtcgcgc aacaacaacg tatcgtccag gtcgatgtaa agactgcgat
      361 agcgcacatt ggtgcgatac cgattgtgta gtgcgcgatc cagctcgacc tgcagattca
      421 gtgtcatcag ccgaatgggc agtctttcgg attcgaaaat gctcaacaag gggaaattga
      481 cgccctgaat acgatgagcg ctcatcgagc cggcaatgcg gggagcgact tcgagcagtg
      541 tcagcgcgcc atcgctcgcc cttttgagct ggaagaacca gccgcctcgc aaagcaagca
      601 ccgactggat gcgtctggct atttcttcgg cctcgggcag atccaccaga cgcgtgtgca
      661 cggcaattcc gttgcgcatt cgcaaccgga ttcgcgctcc acaaaacagt acgccacgct
      721 cgcgatcact gaaacagtcg accgtgtatt cctccccggg cagatactcg gtcacgatcg
      781 gatccttgac tgcgcgtagc gcggtttcca gttccccggt gtttctgacc ataacggcgc
      841 cctgggatcc ctgtccacgg tcaggcttga cgaacagggg aaatggaccc gtcgtatccg
      901 gcgcgtacag ctgcggaacc cggacaatcg gagcaaggct ttgataggtc aacgtcttcg
      961 agcgcgtgat gcgacaggtt tcttttggcg acgtgaggat tgtcgccgga atttcgtcag
     1021 ccagatcggc gagcgccacg atcacgtcgt cataagccgg gaagatataa tcgattttcc
     1081 tggtctgaca caatgtagcc agttgcttgt gccaatccgg ttcatgaatg gttggcaaga
     1141 catcataatg ttcatatacg aaaggggcat gactggaaat atcctgccct gcaccgaaca
     1201 actcgacttc cttgcaatct ttcaatgcct gatggatttc aagcccgatt tcggtgcccg
     1261 caggaaaaac cagaacacgc cggacagtca tttcggcttc ccggacatgt ttttctcctc
     1321 ttcccgcggc aacgtgatca cgagaggcct cacgggtgaa ggatcatgtt gatgatgcgt
     1381 tcctgatcat gtccgctcag tgccggataa atcggcaggc acaacacctg gaacgccatt
     1441 tcggtggcta cgggcaggtt ttcgcgccgg gcggaaggca gcccgcgata catggggaaa
     1501 tcagagatca gcggatagaa atagcgtcgg gcgtagatgt tgtgttccct gagccggtca
     1561 ttcagttcat cgcgcgacaa gggatattcg gcaccgacca gaatgggaaa ataggcgaaa
     1621 ttggcggtgc ggacgtctgc tgccggcagg aaacggatgc cggggatgcc cgcgagctgt
     1681 tcgcggtagg ccgcgtcgat gcgctgccgt tcggcaatcg cctggtcgat gtatttcagc
     1741 tgcagcagac cgagcgcggc attgaattcg ttcatcttgc cgttgatccc gggcgcaacg
     1801 actgttactt catcgacata gccgaaattc ttgaggtggt ctatgcgagt ttttgttttg
     1861 gcatccgggc agatgatggc gccaccttca aaggtattga acaccttggt ggcatgaaag
     1921 ctcagcaccg aaagatcgcc atggcgcaat acggtgccgt cggcatcgtt cacgccgaac
     1981 gcatgggcgg catcgtagat gaccttgagg ttgtagttat ccgcgatatg ctggatggca
     2041 tgcacatcgc agggacgccc ataacagtgc accggcatga tcgcggtggt ctgtggcgtg
     2101 atggcggcct cgatcttggt gggatcgatg ttgaagctgt cggcatcgat atcgacgaat
     2161 accggcttga cgccattcca tagcagcgaa tgcgaggtgg ccacgaacga gtacggtgtg
     2221 gtaatgactt ccccggtaat gcgcagtgcc tgcagcgcgg tgaccagggc cagcgtaccg
     2281 ttggcaaaca gcgagatgtg ttcgacgccg agatactggc ccagggcctg ctccagttcg
     2341 cgatggaatg gtccattgtt ggcgaggatg cggctgttcc agatttgttc caggtaaggc
     2401 gtgaattctt ccagcggtgg caggaacggc tgggtgacat agattggtgg ttcagtgctc
     2461 atggtcaggt gtgcccgcgt gcggatgttc gggtcgtggc gttctggtta cgatatacag
     2521 gatgctggtc tgcgacaagc ccagccgtgt gaacacggtc tgaccgatgg catcgagtcc
     2581 cagctgctga acggcaaagc cattgcgttc gagcgtgctg acatagcccg ggtgcgagta
     2641 cagccgcacg tggtcgttct ggccgaacag gcgccaacgt tccgcgtcgc tggtagcgga
     2701 tgggtcttcc agtgtatcgc tgatgtcgag gcagatgggc gccatcagga tgccgcatcc
     2761 gtcgcgatgc atgatgcggg acagttcgct taccgccagg ttgtcatcgg ccacatgttc
     2821 cagcacatgg ctgcagatga aaaaatcgaa actttcgtct tcgagcggca tgtccatgat
     2881 gtcgatattg taatcgactc ccggcatcat gaaatccgcc gttctgtagt cgaacaggcc
     2941 aagctggcga agttttttcg acaaggctgc ctcgggtgcg aaatgcagca cgcgcatgcc
     3001 cttgttgagt ttgccagcct cgatctgctg attgagccac cagccgtaca accgttcgcg
     3061 gtcggaagca ccgcaggcct ggcaggcgta actgttcagg gcaatcattt ccatgcgacc
     3121 gaaatgacgg aagccatgct gcatcgatgt ttggcggtaa aaatccggca atggggcaaa
     3181 ctggacgccg gagcgttcgc agactgggca gtaggtatgc atttccaccg gaacggctct
     3241 ccccttcatt gtgcagtgtg tgggcgtccc gtgcggcagc gtctgccaga atgatcacgt
     3301 gttgccgcag ttctacggaa ttctcattgt ttgcgccagt gtaacagcgc gcttgcagcg
     3361 tgaccatcat aatatcccgt ctggcacagt ggcaatatcc ggtgctgctg gcggacgcgg
     3421 atgaacttgt tggtgacaaa cgggtcacag gttacatgca gttgcggtat catctggcgg
     3481 aaatggaagc ccgtgtccgg aaatattccg tcccctgctt gaaatccatg gccattgcca
     3541 cgatatcgcg ccttgcagaa ccttgttgcc atacttttgg agtagtcatg tttacacgaa
     3601 caattgccgg tgtgtttctt ggtgccttgc tgctgggcgg caatcccgcc ttcgccggca
     3661 atacgccgac gttgcatcag gtgtatgccg cggcgcagtc cggacatctt gccgaggccg
     3721 aacagatgat gaccgtggtg ctgcagcagc atcctgacag cgccaaggcg cactatgtcg
     3781 atgccgaagt gctgtcacgc gaaggcaagc tggcggccgc gcgcaccgaa ctggcccagg
     3841 cacgccgtat cgatcctgca ctgtcgtttg ccacgccggc agcggtaagc gcacttgaaa
     3901 ccagcctgtc aggttcggcc acggtgccgg cgacagccgc cgtgcaacac cgtgcgggct
     3961 tcccgtgggg gttggtgctg tttggcctgg ctgcgatcat gctggtgttc tggatcgtgc
     4021 gcgccatgac ggcacgtcgc gtggcggtgg tctcccccgg cccgtattat tccggtccga
     4081 acggcatgcc gggtacgcct tatggcaacg gccccatggg cggcgcgccc tacggcggtg
     4141 ctcccatgat gccgatgggt ggcggggccg gttcgggaat catgtcgggc ctggtgactg
     4201 gtgcggccgt cggtgccggc atggtggccg gcgaagcgct ggcgcaccgt ctgatggatg
     4261 gtggttcgtc gaccggatcc ggtgtggtgt ctgacgcctc ggccaacgac tggaccgctc
     4321 cggccaacag cgacatgggc ggcgcggatt ttggcgtgag cgatgccggt tcgtgggatg
     4381 acggttccgg gttcggtggc ggcggcgatg actggagctg atgccacagc cacttgcatg
     4441 acggtgcgat gaagcttcgt tgcgcgatgg ttgccttgtc cggggcgtgt gccgtccggg
     4501 agtccgtgcg atgatctccc gcgtgggcat cgaccgcaac tggttgctgg ccgtgctcga
     4561 caatgcgctc gacgaggtct atttcgttga ctgcaccacc ggtcgttttt gctatgccaa
     4621 ccagaccgcg ctggacaatc tgcaatacgg tttacctgaa ctggaaggca tgacgccgct
     4681 cgacctggcg ccggaactgg gtggtcagac gtgggatggt ttcatgcacg ccctggaccg
     4741 cgaccatcag gcggtgctgc attacgaagc gcgccagcat cggcgtgacg gtacggtcta
     4801 tccactggca ttgcgcctgt tccgttgccc ggctgccgag ttgccggtct acattgccat
     4861 tggcaacgat cagagtgcgg cacgcgcgac ggccagcgcg ctgcagcaga gcgagtcacg
     4921 ctaccaggcc attgtctcca acattccggg gctggtgttc cagtgccaca ggcaggcaaa
     4981 cggcgagatc acttttccct acctcagcga gcgcagcagt gacctgctgg gtgtggagcc
     5041 gcaggtgttg caggcgtccc ctgccctgtt ctttgacctg gtcatggccg acgatctgca
     5101 aggtctgctg cgcgccatgg aaatatcggc acgcaccgca agtaccctga actggcaggg
     5161 gaagatctgg atcgccaact ggcaggacgt caagtggatc agcatccggg caacccccca
     5221 ttgccttcct ggcgatgaaa cacgctggga cggcgtcatc accaacatca cccagaccaa
     5281 gcatgaggaa atggagatca aacgttccca tgcgcagatg gcggagcttt cagcgcacat
     5341 ccagtcgctc aaggagcagg agcgcgcgca gatcgcacgc gagatccacg atgatctcgg
     5401 cggcaacctg acagcaatca agatggcgct ggcgctggtc aagcgccgat gcggtgccga
     5461 cgccgcctcg ctggaaaaag tcggttatgc cgaacatctg gtcgatcgca ccatcgagtc
     5521 gattcaccgc attgcgggtg atttgcgacc cagcgtgctt gactttgggc tggtggccgc
     5581 catcgaatgg caggcacgcg aatttgagcg cctgaccggc atggtctgcc acgtatccag
     5641 cgaacaggaa gacgttggcc tgggcagcga agcctctaca gcgctgttcc gtattttcca
     5701 ggaagcgctg acgaatatcg gcaaacatgc gcaagcgaca caagtggatg tagaattgca
     5761 cctggacgaa acatgggtgt ggctggaaat cgtggataac ggtcgaggca tgcagcagtc
     5821 ggatcggctc aaacccgatt ccttcggttt gcgcggcatg atggagcgcg tggattcact
     5881 gcatggcctg tttgatatcg aatcctcgcc cggcatcggg acccacctgt cgattcgtgt
     5941 tccagtcgaa ccacagatat tcagaaatga tgataaaacc ggacaagatc agggtatata
     6001 tcgttgacga tcacgccatc gtccgcgaag gggtgaaaca gattctggcg gacacgccgg
     6061 acatggtctg ggcgggcgat tccgacaatg gccatgaggc catacgcatg gcacgccagg
     6121 gcggattcga tctgctgttg ctcgatatct ccatgcccga gcgcagcgga atcgaagtgc
     6181 tcaagcagat ccgcaaggaa gtacctgaaa tcaacgtact gattctgtcc atgcaccgtg
     6241 aagaccagta tgccatccgc acgctcaagg cgggcgcatc aggttacctc aacaagcaga
     6301 gcgcaccgga cgaactggtt ggcgcgatcc gcgagatcgt ggcaggacgc aaatacatca
     6361 gcaaggaagt cgcacaggcc ctggccaacc aggtcagcgg cgatcaccag acggcgccgc
     6421 acgaaacact gtccgatcgt gagtaccaga cgttgaccat gatcgcctcg ggaaagtgcg
     6481 tgagcgacat cggcaaggag ctcaaccttt ccgtcaagac ggtcagcatg taccgttcgc
     6541 gcctgctgca caagatgaaa ctgaaaaaca acgctgaact catgcactac gcgatgcgca
     6601 acaaactggt ctgaggtgga cagattgtgt ccggttgccc gatgttcatg atcgggaacg
     6661 gaagccatca tcctggatgc gatcgtctat tggcggcggt agaatcgctt tcgcttccgg
     6721 gtgacatcct gcaaggaaag ccatcatttc agtggcgtcc ttcgacagat agaaatccac
     6781 catcttctcg ttgaactcct gcgcctttgc ggcgggcacg ctgatggcgt cgataccgtg
     6841 cgacatgagc acgccgttca tcatgaagcg cgaggtacgt ttgttgccat cgaagaagaa
     6901 ctgctgcaag gcaccgaaca ggaaaaaggc cgtcgcccgc tcgaacggtt cacacgcttg
     6961 cagggctgaa acgccttcac tgaacacctg gttcagttcc ggcgctccgg gcagcgtcgg
     7021 cagcggggta taacgaccgc gctcaccgag gcccacatcc ggggtgtagt tcttctcctg
     7081 cccttcgcca cggaacatgc cccattccag ggcttccttg cgggcgacaa tgccgttcag
     7141 ttccgcgaat accggcttgg agagttcaaa ctggccgctc ttgaccatcg ccagcagtcg
     7201 cttggcgcct tccgccagat tcaggatttg ttcctgatcg gaaagcttgc gcccgccgat
     7261 ggtcacacca tccagcagcg tctggacctc gggaaaggtg aagggattgc cttcgagcat
     7321 cgatgcgtcc cagacgaact ccggcagcat cttgtggaag cggaagcaga cccgatccag
     7381 cgagtgatgc ggaacggaag ctggcaccgc cgatctgtcc catcggaagc caagcgcatc
     7441 aaagagggtc atatcgcagc cagtcaatca ttgaatacgg cgttatggta gcagcaatac
     7501 acccccgaaa cacgcttttg cattgcggcg cattcagcac cgaatgcagt acttcaacca
     7561 ggaaaaccgg ccccattttt ttcaaaaccc tgaagtaaac ggaattcctg ccgttaatca
     7621 cgcgattctc tcgcggcacc gctgtgtatc atgaatctcg agtggaccgg agcctgtggc
     7681 ttcgcaagca agagaaagtg acatcatgac caacagtgcc tcgcaaagtc ctgccgaaat
     7741 cgccaaggaa gccttccgcc gtctggcggt gcagcgcatc gctccgacac cggaagccta
     7801 tcgcaacatg tacgaagaaa ttgccggcgt gcagaaagcc gtgtcgcccg aacgggtgct
     7861 ctcggggttt gcggtgcgca tgagccagcg gcccggcgaa ctgtcgctgt tcggcaagcg
     7921 tctggcgcga gcggcagaaa gcggtaactg gcaggaatac caggatgacc tcgctgccct
     7981 gctggacgag caggagaaga ccgttgctgc gccacccccg gcccccattg ccgcagcgcc
     8041 ggtcgaatcc gtggggatct cgccacaact caagctgttg cgcgagctgc tcaaccgtac
     8101 actttctttt gcaatgccat cactgctggc caatgttccg gaacttgcag cagaatcgag
     8161 cgcgctgggt tcggccgtca aggaagcgcg cagcgatgtc gagctcaacg aaatcggcaa
     8221 tcgcctcaaa cagctgtgtt ttcgcatcga gctcaagagc ggtgacatca atgagcagca
     8281 ggaaatgctg ctgcgtctgt tccgcctgct gctggacaac attgcttttc tgctggaaga
     8341 cgatagctgg ctgcgtgggc agatccacgt ggtgcagaac ctcattgacg gcccgatcaa
     8401 ttaccgttcg ctcgaagatg cttcgcaaag cctgaaagaa gtgatctaca agcagggaac
     8461 gatccgcgac agcctgtcgg aagccaagat caccgtcaag aacatgatcg ccattttcat
     8521 cgatcgcctg ggcagcatgg ctaccagcac cggtgattat cacgaaaaaa tggacacgta
     8581 ttcaaagaag attgcccagg cagaaaacat caacgaactc aatgagatcc tcagtgatgt
     8641 catgcgagat acgcgcacga tccaggcgga agccctgcag tcgcgcgatc agatgctcag
     8701 cgcccatcag gaagcccggg atgccgaggc acgcatccgt gcgcttgaag cggaactggc
     8761 cgaagtcagc gccctggcaa gccacgacca gctgaccaac agtctcaacc gtcgcggcct
     8821 ggacgatgcc atggagcgcg agcaatcgcg ttccgagcgt caaggcacca gcctgagcct
     8881 tgccctgctc gatcttgacg atttcaaacg gctcaacgat gaacatggcc atgaagccgg
     8941 cgatgaagcg ttgattcatg tggtgcgcat catccgcgac acattgcgct ccatggatgt
     9001 cgtgggccgt tttggcggcg aggaattcat cgtgctgttg ccggacacgc cttcgaacga
     9061 agccgcatcg gtggtgagcc gcctgcagaa ggagctgacc aagcggattt tcatgtacaa
     9121 caaccagaaa ctgtttctta ccttcagcgc tggcgtggca acccgccatg ccggtgaaga
     9181 tctgcgcgaa accatcaagc gggcggacgc ggcaatgtac cgtgccaaga aggccggcaa
     9241 gaaccgtgtg gtgatggctg acgacggcct gccgggatga ccggatgcct cactgtaaaa
     9301 gactggcgca agccggtcta aagtcaggcc gtttttggcc gttaactggg ataggcggta
     9361 gagtaactgt tgaaaatttg cgcagaaata tttgattttc aaggctaaat tttctcgcgc
     9421 accagtcgat aaagacctca acagcggtgt gatcgcattc ggacacaaca cagttcggac
     9481 acaacacagc gagccactgt taaagctgag tcactgagtc acagttacca aggagaaaca
     9541 tcatggcatc ctatctcaat acaaacatgg cttcgctgat tgcccagaac aacctgagca
     9601 attcgcaaac ctcgttgacc acctcgattc agcgcctgtc ttccggtctg cgtatcaaca
     9661 gcgctgccga cgatgctgcg ggttacgcca tcgcccagaa catgaccacc cagatcaacg
     9721 gcaatgccca ggctgccaac aacgccagca acggcgtgtc gcttgcgcag accgcccagg
     9781 gcgatctcgg caccattctt gccaacctgc agaccatcag cggcctggcc gtcgaagccg
     9841 ccaacgcaac caatacttct gccgaccgcg ctgcgctgaa tgatcaggtg cagcagctgc
     9901 tgcaggaaaa caaccgtatt gcccaatcgt ccaacttcaa cggcgtcaac ctgctggacg
     9961 gttccttcca ggcacaaacc ttccaggtcg gcgccaataa ctccagcgct gaccagatca
    10021 gcgtaagcgg catccccagc atgctgacct cgcagatggg taacgtgacc accggtgcca
    10081 cggtgaccgg cggggctacg actactgcat tgacggcggg tcaattgacc ctcaacggcg
    10141 tgcaggtcgg cgcttcggtc gcaggcactt ccgcaggtca gaccaccgac agcgcctatt
    10201 ccatcgccca ggccatcaat gcggtcgagt cgagctccgg cgtcaatgcc acggccaatg
    10261 ccgcgaccgt ctcgggaacc gcaactgcga ccacttctgg cgcggctggc gcgctggtca
    10321 tcaacggtat ctcgatcggc gccatcactg ccggcggaac ggcttccggt gaagccgcca
    10381 acgttgccgc tgcgatcaac ctggtgtcgt ctgaaaccgg cgtgaccgcc acggccagtg
    10441 cttcgggtgc cggcatcacg ctgagcgctg ccgatggtcg cgacatcacc gtcaccagcg
    10501 gaaccggctt cagttccgga tggggggttg caaccggcac cacccacggc acgctcacgc
    10561 tgaccagcag cagcgcgaac ggtattacca tcagcggcag cacgcccggc aatgccggcc
    10621 tgactgcggg taccaccgcc gcttccagcc agctgaacag cctgtccaag ctggatgtgc
    10681 tcacggtgac cgatgcaacc aatgcgatga cgacgattca ggcggccatc aatcaggtta
    10741 caacggctgc ggctcagatg ggtgcatatc agaaccgctt ctctgcggtg gtgaccggca
    10801 tccagaccga tagccagaac ctgacccagt cgctggccac catccaggac accaactatg
    10861 catcagaaac cgcaaacctg agcaggctca acatcctgtc ccaggcgggc aacgcaatgc
    10921 ttgcgcaggc caatcagctt cccgcccaga tcctgacgct gctgcgttaa gatcagtctg
    10981 gaattgatag cgcattgttt taaccggcag aaagtggcgg cccactttct gccctcccat
    11041 ccgggagaac tgaaatgaat atcggacaaa tcactacatc ggatgtgacg ccagcaccgg
    11101 ttcctgtggt ccctgttgcg gtcgcgcaaa gctcagggca ggtcgcacag gttcccgttt
    11161 ctccggtccc ggtgtcggcg accccagctg cagccagcca ggcacagccg agccagcaag
    11221 cattgcagga ttcggtgaac aacatcaaca atcacctgaa ttccttcaac aacaacagtg
    11281 tccagttcag tctcgattcg tccaccggac aggtcgtggt gcaggtcgtg gatacccaga
    11341 cacaggcgat cctgatgcag tttcccagca agcaggcgct ggcgattgca cagtcgctcg
    11401 aaaaatcgca ggggctgctg atccagacca aggcgtgagc ccgggtcctg ttccagcatg
    11461 actgaatcgc gacagaggga cgatcatgac cactaacctg actacaacct ttgcttcatc
    11521 gatcaatgcg gcgctgtctt cgccaggcat cggttccggg ctggatgtgg acagcatcgt
    11581 cagcaagctg atgtcggtgg agcagcagcc actgaccctg ctcgcgcagc agcaatccag
    11641 ctacaacgcc aagatttccg catatggttc cctgcagagt acgctgtcac agtttcagac
    11701 ggcaatgagc agtctggcga atgcgtcgca gtaccaggcg tacagcgcca cttccagcaa
    11761 cagttcggca gtgtcggcca ccaccaccac aggtgcgact gctggcaatt acaacattgc
    11821 cgtatcgcaa ctggcgcaat cgcagcaact ggccaccacc ggtcagtcga gtaccagcag
    11881 cgccattggc agtggcactt cgaccaccct gtcattcacc ttcggctcca tttccggcgg
    11941 cacgctgacc aatggtgtgt acagcaatgc ctcgtttacc agcaatggca atccgacgca
    12001 gagtgttgtg atcaattcgt ccaacaacac cctgtcgggc attgccaacg ccatcaatac
    12061 cgccaatatt ggcgtgaccg ccacgatcat caacaatggc agttcaacca atccttacca
    12121 gttgctgctt acgtcgacgg ctaccggcca gacgagcagc atgcagattt cggtgtctgg
    12181 tgacagcacg ctgagcagcc tgctcagcta caatccggcc aacaataccg gacaggacct
    12241 gacccagacc gtcgcggcgc agaacgccaa catgaccatc aacggcgttg cggttaccag
    12301 cccatccaat actgatacca atgccattca aggcgtgacg ctcacgctgg gtgccaccac
    12361 gggcagcacg ccggccaacg tcagcatcag ccagaacacc agcaacatcg tcaatgcggt
    12421 caacagtttc gtcacggcct acaacagcgt cgcattgacg ttgcagacag acacgtcgta
    12481 caacaccaaa accaagactg caggcccgct caatggcgag gctgcggtca ataacatcgc
    12541 gcaggatctg cagaatatcc tggcgcagcc tgtggccggt tctaccagca gcagcgcgct
    12601 cagcatgctg taccaggcag gcgtcacttt gcagaacaat ggcaccctgt cggttgattc
    12661 gaccaaactg cagtcggcca tcgcggcaaa cccgaacagt ttctccgggc tgtttgccga
    12721 aaccggtcag accagcgaca gccaggtcaa ttatcttggc gccaccagca gtacccagcc
    12781 ggggagttat gcggtcaacg tcagccagct ggctacccag ggggcagcca cgggcactgc
    12841 ggcactggct gcaagcacaa cgatcactac cggcagcaac gatacgctca atgtcacgct
    12901 cgacgggttg accggcacag tgacgctggc tgcggggacg tataccccgg ctcagcttgc
    12961 ggcggaaatc cagtcgcaaa tcgatggcaa cagcaccttc agcaatgccg gctcgtcggt
    13021 gaccgccacc gtcaatgcca gcggcgcact gcaattgact tcgaatcgct atggttcggc
    13081 atccaacgtc aacatcaccg ggggaaacgg ccagtccacg ctcagttttt ccggtgcggc
    13141 gctcaccacg ggcgtggacg tggccggaac gatcaacggc gtggccgcga tcggttccgg
    13201 gcagaccctg accggtgcga ccggcgatgc atcgagtgga ttgcaggttc aggtgaccgg
    13261 tggcgcgctc ggcgctcgcg gcacggtgag ttactcgcaa ggctatgcct atctgctgaa
    13321 ccagtacatg acttcagtgt tgagtccgac cggttcgatc cagtccacca ccaattatct
    13381 gaataccctg gtgcaggaca accagcagca gcaatccacg ctgacccagc agctggcggc
    13441 ggtcaaggca cggtacatgg cccagttcac cgcgctggat acgctggtca gcagcatgaa
    13501 tgcaaccagc agttatttga cacagcagtt gaccaaaacc agtaactaac ggagatggcc
    13561 atgtttggta atgcacggca aggcgcccaa gcctataaca atatcggtat tgaaacgggt
    13621 gtgttttccg catcacccca tcagctgacg ctgatgttgt acgatggggc caaggcagca
    13681 ctcaataatg ccatcatgta catgcgcaac cggcaagtcg cggacaaggg ccgatccatc
    13741 acccacgcca tccgcatcat cggagaaggt ctgcaatcca gcctgaacaa gcaggccggc
    13801 ggagaactgg ccgagaatct tgacgcgctg tatgactaca tgtgccgtcg cctgttgcag
    13861 gccaatctga acaacgacga aggcatgata ctcgaagtca tcggtctcat cgacacgatc
    13921 cgtgatgcct gggtgcagat cggcgatcag tcgcctgcac cccagacagc catgccgcag
    13981 gtacagacag ccccgcttca gggtggatat cttgaatcac aaaccgttca tactcccgca
    14041 atggtgaagg cctgatcatg atgaatgccg aagatgtcat cgtcgcctac gaagatgtgg
    14101 cgcgcattac tgcggaaatg ctggcggcag cgcgcaattc gcgctgggac gagctgatca
    14161 gcctggaaca gcattgcgcc aatcgcgtgc gcacgctggc gcaaggagaa agtcccgtgc
    14221 ggttgagtgg cgaactgcgc aaccgcaaga ttgccatgtt gcgccagatt cttgcagacg
    14281 atcgcgaaat tcgtgacctg accgagccgt ggatgcgtca ggtatcgcag gtcatgagtt
    14341 ccatcaccga gcgcaggctg tctgcaacgt atggtggcgt tcaggccaac tgaaggtctg
    14401 ccacaaccgg ctgcgactgt cccgtcgacg ctggtcacgc cggtttctct ggcggtacac
    14461 gccgccgctg cgtatgacca gaacgaactc gcctttcgcc tgagccagat cgaagtcggt
    14521 cagcagatga ccgcgcagat tcagtcgcag ctcggcggcg gcagctacct ggtcaaggtg
    14581 gccgacaccg tggtgcagat gaccctgccg gaaaccatgc aggccggtga tcaggttcag
    14641 gttaccctga tcgaaaaaaa tccccgcccc accttcctgc tcagcagcga agcggcgcca
    14701 ccgccgcccg agagcagcat catgacgttg agtactgcgg gcaagctggt caccagtctg
    14761 atgcagttgc ccaatgccag ggctgaagcg gcagtgagtg caagtgttcc gctgctcagc
    14821 cagcctgacg cagcgccagc acaactggct gacgcgctgc gtcaggccct gggccagtcg
    14881 gggctgttct acgaatcgca tctcggacaa tggctgttgg gaaaattcag tctggcttcg
    14941 ctccagaacg agccgcaggc ccagcagagc agccagctgc tcatgccgac cgggcaggaa
    15001 cagtccgcgg ccaccaatgc gccctcgccc cagccagtgt cgccacagat accgccggcg
    15061 gtcggtcagg ccggactggc agcggtacag gcggcgctga ccgaaccacc gggcacactt
    15121 cagccggcac cggctgcgcc tcatgcaact ccggacagcg ttgccgacgc gatcgcagtc
    15181 gtgcagggtg ctgcgggcag cagtgcgact gcggccttga cgcagatcgt acagcagcaa
    15241 ttgcaggtgc tggaacagaa ccgcttcgtc tggcagggca tgccctggcc cgggcaacac
    15301 atgcagtggg aaatcgggga gcgctcgccg caaagcgcgt cgccggaata cctgcccggc
    15361 tggaacagtc gcctgagtct gactttgcct gggctgggtg ccgtggtggc cgatctggcc
    15421 ctgaacaacg gccgggtcac cattcgtctg caggcagatt ccgatggtgg ggtacgtcgt
    15481 ctgcgcggtg gccagcaggc gcttgccgaa gcgttcgcca ccgccggaat ccggctggac
    15541 tcggtggtgg tccagcctgg aacaggctcc gggagcgcat gacatggaac cttcgacccc
    15601 ggctcaacag gtggccgtcg ccctggccta ccagacaggc gaagccgcac cccaggtggt
    15661 ggccaagggt aagggcctga ttgccgaaga gatcatcgcc cgcgcccgac agaacggcat
    15721 cttcgtgcac gaatcgaagg aactggtttc gctgctgatg caggtcgatc tcgaccagcg
    15781 tattccgccc gagctgtatc gggccgtggc agaattgttg gcctggatct accgcgttga
    15841 aagcggcgag acggatactg ctccgccaat tacgttataa tgccctgcat cattgtctgg
    15901 atgggacggc atgttgggca cgcaattcga actgggtacg gagaatcaga cgccctacca
    15961 gattcattcg cggcgcgaaa ttctccagtt gctggagggt attgctcgcc agcaccagat
    16021 ggttacgctg tcgattcgcg atggcgcgga ggtcatgctg accaatctgg tcaaagtcga
    16081 cgagtcccgg gatctggtgt atctcgatgc accgcaagac cccgccgtcc tgcacaagat
    16141 tctcacgagc agtgaaatcg gtgtcgagac ggctctcgac aggatcaaaa tcatgttcag
    16201 cgtgccgcat gtccgtgaat gcgaattcgc aggcgcgccc gccctgtgta catccatccc
    16261 gcccgtgctg attcgcctgc aacgccgcga atacttccgg gtcaatacgc cgtccgacaa
    16321 aaaactgtat tgcgaaattc ccctgccgca aggggtgcag caatttgcgc tggccgacat
    16381 cagctgtggc ggcatcgcca tgatggatga acgccgcctg ctcgccgccc acgtcggaga
    16441 ccagttcgac aattgccgca ttaatctcgg cgacgcgggc gtattgctga tcacactgca
    16501 aatccgcaat tcgatggacg tcgcgctgct caacggcaaa aacacgcggc gcgtcggttg
    16561 ccggttcatc aatcagccgc cgggtatgct gaccattgtg cagcgttaca tcatgcagct
    16621 cgaacgcgag cgcaacgcgc gcatgtccgg cctgttctga cgcagttccg aacccgtgtc
    16681 cacgcatcca ggcggctccg gagccgatcg cccattcacg gtattgcaga tgctgccggc
    16741 attgcacgca ggcggtgtgg agcgtggcac gctcgaaatt gcccgcagcc tggtcgcaca
    16801 gggacaccga tcgctggtga tgtcggcagg cggcagaatg gtggaacagc tggaaaacga
    16861 aggttcacgg cattttacct gggcaatcgg gaaaaaatcg ctgttcacgc tgcggcaggt
    16921 tggaccgctg cggcgcttct tgcaggaaca gcgggtcgac atcctgcacc tgcgttcgcg
    16981 catgcccgcg tgggtcggct accttgcgtg gcgaggaatg ccagccgcca cccggccgcg
    17041 cctggtgact acggtacatg gcccctattc catcaatcgt tacagtgcgg tcatgacacg
    17101 cggtgaacgt gtgattgccg tgtcggacac gatccgcgaa tatttgctgg cgaattatgc
    17161 gcaactcgat ccggcacgca ttcacgtcgt gcatcgcggc gtcgatccgc acgaattctc
    17221 cccccgcttc cgcccggccg atgcgtggcg gttgcgctgg tacaaacaat accctcgtct
    17281 cgaacaggcg cgggtgctta ccctgcccgc gcgactcacg cgctggaaag gccaggtgga
    17341 tttcatcgaa ctgatcgcca gcctgcgcgc atcaggattg ctcgtgcatg gactcatcgt
    17401 tggtgacacg gctgcgagca aacggcatta ccgtgccgaa ctggaacagc gcatccgcga
    17461 ccgtgggttg gaagacgcca tcatcctgac cgggcatcgc tccgatctgc gcgagatcat
    17521 ggccagttcc gatctggtgc tgtcgctgtc acgcgaaccg gaagcttttg gccgtacggt
    17581 gccggaagcg ctcgcactcg gcattccggt ggttggttat gcccatggtg gtgtaggaga
    17641 agtgttgcgg gcctggtacc cgcaggggca ggtattgccg ggcgacaccg atgccctgtc
    17701 gcaccgggta cgcgagctgt tgcttgaacc gcgcccggtg ccgccacaga cagacttcac
    17761 tctggcagcc atgcaagcca gcacgctgga actgtatcgc gaactgctgg cggagccacg
    17821 gtcatgaatc tggcaggacg gaagatctgt gccgtggtgg tcacctggaa tcccgacgat
    17881 gcgttcccgc agcggctgga agccatccgg gcgcaggtcg actgcgtggt gatcgtcgac
    17941 aacgcgagca cgggcaacag tgcggccatg ctcagggcat tgtgcccgtc gcaccagtgt
    18001 ctgcgcaatg acgaaaatct gggcgtggcc agcgcgctga atcttggtgt gacatgggca
    18061 gacgctcacg gttatccgcg cgtggtgctg tttgatcagg acacgcaagt cgatgacgga
    18121 ctggttgagc gtctgtgcga tgaatggaag gcatggtccg gtacggcgcc cattggcgta
    18181 cttggcgcca gatttgtcga tgtccgtcgc cacgtatcgc ttgcgcagtg cgccgatact
    18241 gccggcagtg acgcagtcga ttgggtcatc acttccggtt cgctgctgct ggtcgaactg
    18301 tggcgcaaac tgggcggttt ccgtgacgat ctgttcatcg atttcgtcga caccgaattc
    18361 tgctggcggg tccggcacag cggttacgcg gtcctgcgca cccgcgccgt actcatgcaa
    18421 catgcggtgg gcgcctaccg gacacatcgt ttgttggggc gcacgacatg cagcagccac
    18481 cattcggccg atcgacgtta ttacatgaca cgcaatttca ttatcctgct gcgcgaatcg
    18541 ggcaactatc cagctggcgg ctggctgctg cgcggcatgc tggccagctt caaatcggtc
    18601 aagcggatcc tgttcttcga acaggacaaa cgtgccaaac tggctgcggt gatccaggga
    18661 tggtatcacg ggttgcgcaa ccgtaccggc ccgcgcttgc gtccgctgtc gccgcaccat
    18721 cgcggagcgg cattccgggt gagcagactg tcgtgaccat gcacactacc ataacgaccg
    18781 gccattcggc acgcccgagg gtgatggtcg ttggcaagta tcgcagtatc gtgcactggg
    18841 cggaaaatgt gtcgtcggca ttccgccaga acggctgcga ggtggcgcag tttgccatca
    18901 acggccagac ggtctggcat tcgttgcgtt acaaatttgc cggcaacgag tatggcagtc
    18961 aggcgctgcc ggttcgtgcc gattttgaac gtgcgctgca gcggttcaag ccggatctgg
    19021 tggtgttcat cgtgattgcc gccctgtgga tgccggaaga actgtttgcg ctggcacgac
    19081 agacacggcc gggtgcggct acagccgtgt ggatcggcga caggctcaac gcggaggaat
    19141 gccgctttgc ccatcatgtg gatcaggtct tcgcgaccga cagcgccttc atcgatgacc
    19201 tgcgccacta cggccatgcc ctgcccgcac attacctgcc gctggcggtc gacccgcagc
    19261 gttttcatcc gttcagtctg gcgcgatcgc aacggctggt gtacgtggcc aacaacagcc
    19321 cagggcgcgg tgaattgata cgccaggtgc gcgaaccgct ggtgctgtac ggcaaaggct
    19381 ggtcaaaact ggcggacacg gcgcacgata tccatgcgcg ccgcctgccc taccgtgatc
    19441 tgccgcgagt gtatgccagt caccgtgccg tgctgaacat ccgcaacgag aaaaatgtac
    19501 ttcatgggct gaaccagcgc agttttgagc cctatggctg catgaccccg gtactcaacg
    19561 atgatatggc ggatctgcca cgctgcttcg aaccgggcaa ggaaatactt gtctggcatt
    19621 cgcttgccga gctggaagac ttgcagtccc ggctgcagcg cgattccgga tttgcccagg
    19681 cgattggtct ggccggtcac cgtcgcgtgc tggccgaaca cacctggcaa caccgcatcc
    19741 attccctgtt gactgctttc gattttcagg acgtccgttc atgaatgcat accccactcc
    19801 ccgtcttgaa cgcgttgcag gctggcttac cgtgctgatc ccgccggcgc tggtgatcgg
    19861 taacgctgct gctgaaatcg tggccggact ggtggtcatc ctgtttgcct ggcgctgtat
    19921 cgtgctgcgg gatggcagct ggctgcgcca gggctgggtg aagatgctgc tggcggtgtg
    19981 ggtgctgggc gtgacgcgcg cactgctcac agaacatccg cacaccgcgt tgccggaagc
    20041 cgtgggctgg ctgcgcttcg tcgtgctggc cgtagcgctg cagacctgga ccctggccga
    20101 tgaacgctgg cagcagcgcc tgaccaatgc cggattgctg tcactggcct ggctcgccgg
    20161 cgacgccatt ttccagtatc tgcatggcac tgacctgttt ggccggcgga tcctgtttcc
    20221 gcaccggctg accgcctcat accccaaacc gatcgttggc atcatgctgg cctggcagtt
    20281 tgtgccctat gcgttcggtg atctggcgcg cggccggtcg ctgcgcgcgc ttgcgatggg
    20341 tgtgctggca ctggtggcca tcgtgctgag cggcgagcgc atggcattgc tgctcagcct
    20401 gatgtcgctg ctgctgctga tgctggccct accctcgctg cgacgcgccg gggtggcgat
    20461 cctgctgctg ttcgtgctgg gcacgggcgc actgatgctg gccaaaccgc aactgtacga
    20521 ccggcaggtg acttccacgc tgaccgtgat ccgcgatctg cccgattcgc cgtacggtgt
    20581 gatctggcgc agcgccatcg acatcgtcgc cgatcacccg ctcaccggag tgggcatggg
    20641 taatttcggc catgtgtgcc cggaaccgca gtatggaccg atcaacgccg gggcgttcgg
    20701 ttacccgcgt tgcgcaccgc atccgcacaa catctatctg gaatggctgg ttgagaccgg
    20761 catcgttggg ctgaccggtt ttgtggtcgc catggtgctg gtgctgggcg tgctgtggcg
    20821 ggatatgcgt cggcattccg gcgatatgct gtctgccgcc ctgacagtca ccctgttcgt
    20881 gcgtctgtgg ccgttggcct cgagcaccag ctttttccat ggctggtcag ccattccatt
    20941 ctggctggtg ctgggctggg cgctggcgcg ggtccgtccc atgcgcgaga cggcatgacg
    21001 ccatcgatcg cgattctgtt gccaccgcga gaggcgcatt ccgggcgtta tcgtggtgcc
    21061 gtcgcgttgt gcatggcaga tttcacccgg tttagccgct accgtgagcg gaccgtgatc
    21121 tattgcggcg agcaggcgta ttttgacgac ctggcgcatg tgcagttgcg gcaatggaag
    21181 cgctggtatc tgcgcgacaa cttcgcctgg gccagggcat ttgcccggga atgccgcgca
    21241 cgtggccaca ctcacgtcga agtgcaaaac cgtcccatga tgttgcacta cctgcgctgg
    21301 atgttgccgg aacgcataca actgagcctg cacctgcaca atgacccgca aaccatgcag
    21361 ggttcgacca gcgtacgcga gcgccagcgt ctgctcgatc gcgcggcggc agtgtactgt
    21421 gtgagtgact atgtgcgcga gcgctttgtc gagggtctcg atcatggtgc cgacaaagtg
    21481 catgtggtgc acaatggtat cgatacggtg gcatttgcgc cgcaaaccaa gcaacaggtg
    21541 attgtgtatg tcggacgcat gatctggcag aaaggcgcct tgccattgct ggaagctttc
    21601 catggcattg ccgcacaact gcctgaatgg acactggtgt tgtgcggcgc tgacagactt
    21661 gaacaaacct ctgaatatga gcgcgcaact cacgaaataa tcacaaaaat tggctcacag
    21721 tgcgtataca ccggttatgt tgatcatgcc gcggtgatgc agtattttgc cggagctgcc
    21781 atcgctgccg tgccgtcggt atggcaggaa ccgtttggcc ggacagccct ggaagccctg
    21841 tgtgcgggtg cagcgctgat caccagcgcc agtggcggca tccgtgaagt gatcgacgaa
    21901 cagtgtgcac tggtcgtcga tccggatcag ccggactcga tcgctgccgc gctgctgcgt
    21961 ctggcaaacg attccggact gcgcactcgc ctgcagcagg ctggcagaca gcgtgcggtg
    22021 gaacatttcg acatccgcca cgtggccgcc cggctggatg atctgcgcga cgccctgtcc
    22081 acctgacgga accagtcttt tcttgtatgt gacatgtttt aattattcga
//
LOCUS       DAIANL010000049         8206 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1680374, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000049 DAIANL010000000
VERSION     DAIANL010000049.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8206)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8206
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680374"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1507 a         2315 c         2759 g         1625 t
ORIGIN      
        1 agcccagggt ggtggcaccg ggatggctgg cggtgggcag taccggcgat agcagggcgt
       61 agtcgcagcc gaactgctcg gcgcgatcga gttcggcagc atcatgacac gacgcaccga
      121 tcaggggaaa atcaggacgc gtgtcgaagt tggccagagc ggttgcgctg aggtgcagtc
      181 cgtcggcctg taccctgtgc gccagcgcgg catcaccatt gacgctgacc agagcgtggt
      241 gccggtgcgt gagtcgaaca gcctcgcggg cgaagtcttc gatgtcgggc agctggtttt
      301 cgcggatgtg cacccatttt agtccccgtt gcagggcgcg ctccagtgca gcgagaaagt
      361 catcaccccg gccttccgta tgactgatgc cgatgagtga cggtaactgc caggcacgta
      421 gcagtcggtg attggccggt aacagcggtt cgagcgcagg tgcgagcggc gattgccagg
      481 acagttgctg cccctcgcgc gactgcggtt caccctgcca gccgtgcacg cgaaagcaat
      541 gcagttccac tcgggtgtgc tggtagtcgt ggcgaagcac gatccacggg ctggcgtcga
      601 cgacgcggat gccgagttcc tcttccagtt cgcgttccag ggcatggcgc ggggattcgc
      661 cggtttcgat cttgcctccg ggaaattccc agtaccctgc ccagggcttg ccggcaggcc
      721 ggctggcaaa cagcagcgta ccatctggcc ggcacaggat gccaactgcg acgggaacgg
      781 tagggcgcgg tggtatagag gacatgatgg tcttaaatat tgtgttagta ttctatgata
      841 caggttgata aaagcgaaaa tgcatgtcga tagaattgtt caacgaagat caccatgtct
      901 gcatgatgtt cagcgatctg gtcgatgata ccgcggatgc ggtgcagaca aaccagtttc
      961 tcatcgtttc ggatggcgaa ggtgcgctga tcgacccttc gggcaacatg acctacaacg
     1021 cgctggtggt tgccatgggc aggtattttc cttacaagcg tctcaagtac ctgctggcat
     1081 cgcaccagga tccggacatc gtcgggtcgc tgaaccgatg gatgatgtct accgatgcgg
     1141 cgttgctggt gtcggatctg tggtcgcgtt tcgtgccgca tttttgcacc gccgaccgta
     1201 cttcggggcg tctgatcggc atttctgacc gtggtatgcg tattgcgctg ggcaaggatg
     1261 aaattatcgc cgtgccagca catttcctgc acgcggaagg caatttccag ttttatgacg
     1321 tgcgatcgcg cattctgttc agcggtgata tgggggcttc gctggtacac cacgacagcg
     1381 tgccgcaacc gatcaccaca ctggaggaat tccgtgctca tgtgcgctac atggaagggt
     1441 tccaccaacg ttacatggtg tcgcgcaagg tgtgcggtta ctgggttgag atggtgcggg
     1501 cgatggacat cgatgcgctg gtgccgcaac atggtcgctg gttcaaaggc aaggtggcgg
     1561 tgggcgcgtt tctcgactgg gttgccgggc tcgagtgcgg catcgaccgc tttactcagg
     1621 cgcagtacca gttgccgtaa gtcgcgtgcg gccggcgagg tcgcgcacga actgccacgc
     1681 gctgcgaccc gagcgcgttc cacgcatctg cgcccattga agcgcctcac ggcgcaccgt
     1741 gtctgcatcg agggcgccca gaccgaacgc atccacccat tgtccgacgc aggcaaggta
     1801 ggtgtcctgg tcgaatggcc agaatgacag ccacaggccg aagcggtcgg acagcgaggt
     1861 tttttcttcc acggtttcgt tcggatggac ttcggcaccg cgtgtcgggc cggactggtt
     1921 gtcggccagg aattccggca tcagatgtcg ccggttggag gtggcataga tcagcacgtt
     1981 gtccggggtg gcggccaggg agccatcgag cgcaaccttg agcgccatgt agccgttgtc
     2041 gtcgtgatcg aagccgaggt catcgcaaaa caggataaag cgccacggtt gtccggccag
     2101 caggggagtg atgtcagcca ggtcggccag ctgggacttg tccacttcga tgatgcgcag
     2161 cccgtgaccg gaaaacagcg gcagcatggc ttttaccagc gacgattttc ccgtgccacg
     2221 tgcgccggtg agcaaaacgt tgtttgccgg atgtccggcg acgaactggg cggtattgcg
     2281 cagcagttgc tgcttttgcg cctcgatgcc atccagccgg ccgatttcca tgcgttgcgg
     2341 ctgagcgatg gcttcgatcc agccctggcg gccctggcgg cgccagcgga aggcgctgtg
     2401 cgcggcccag tccggcgtgg ccggttgtga cggcagcccg gattcaagcc gttcgagcag
     2461 gtgctcggca cgatcgagaa agcggctggc gcgttcggtg agttcagctg cggtattcgg
     2521 cattgatctt gacgtagtcg taggagaagt cgcaggtcca cagcgtggcc cgggcatcgc
     2581 cacgatgcag cgcgacgcgc acggtgatct cgtcttcacg catgacccgc tggccatcgg
     2641 cttcgcggta gctggccgcg cgaccgccgt gttcagcgac cagcacgtcg cccagccaca
     2701 attgcagtgc gctgacatcg aggtcgtcga cgccggcgta gccgatggcg gcaaggatgc
     2761 ggccgaggtt gggatccgaa gcaaagaaag ccgtcttgac cagcggtgaa tgcgcaatgg
     2821 caaagccgat gcggcggcat tcctcgaccg agcggccact ttcgatgtcg atacggatga
     2881 attttgttgc accttcgcca tcgcgtacga tggcctgagc cagttcgatg gcgagttcgc
     2941 ggatggctga ctgcacagcc gtgtaagccg ggtcggattc gtgttcgatg gtgcgcgcac
     3001 cggcggcgcg accggtggcg atcagcatga atgcatcgtt ggtcgaggta tcgccatcga
     3061 cggtgatgca gttgaaactg gcatcggcgg catcccggac catacgttgc agcaatgccg
     3121 gcgcaatggc cgcatcggtg gcgatatagc ccagcatggt agccatgttg gggtgaatca
     3181 tgccggatcc cttggccatg ccggtgacat ggatggtcgc gccgtcgacg gccagacggc
     3241 gtgatgccgc cttggcgacg atgtcggtgg tcatgatggc ctgtgcggcg gcgagccagt
     3301 tgtcttcacg cgcatcggca agcgccgccg gcagcgcgtg aatgattttt tcgtgcggca
     3361 gcggttccat gatcaccccg gtggaaaacg gcagcacggc tgtgcggtcg caaccgagca
     3421 gtccgcccag cgcgtcgcac actgcgcccg cgcgcatcag cccgtcatcg ccggtcccgg
     3481 cattggcgtt accggtattg atgaccagtg ccgcggggct ggacgcatac gccagatgct
     3541 gccgtgtgag cacgaccggc gccgcacaga aacggttgcg cgtaaatacg ccagcaacct
     3601 ggctgcccgg tgcgagcaac agcacagtca ggtctttgcg gcctggcgtc ttgacgccgg
     3661 cactggcgat gccgatacgg attccggcaa cgggatgaag gtgatccgcc tggggcggtt
     3721 gcagtcgaac aggcatgcgg gctccggcag ggaatcagga tttctggcgc agcgcttcgt
     3781 cgttgtaggc gccatcgatt ttatgcacga tgtcttctac catgcggaag ccttccaccg
     3841 gggcatcatc cttcatgtag tgcaggcgca ccggtcccag cgccttgact gcctggacgc
     3901 gctccagagc gtctcccggg ctggcgatct ggctgttctg gtagagctgg gcgaggatgt
     3961 ggccggccac cgggtttttg tggctgccat ccagcgccgc attcagtgtg tggtcacggt
     4021 cgccgtcggc gatgtcgtat ttgctgctgt gcagcaggta gtcgagcagt tcgttaaaag
     4081 tcatgtgatt tcctcgcagc cgtggcatcg tccggtgtcg gaccgggttc gttcagtctg
     4141 gtttcgataa acaggtattc gcgcgcatcc accggcgtga tggacagtcg gttgccgcgc
     4201 tgcagaatac gcaggctggc aagctccggg caggcgcgca gttcggacag gggcatgagc
     4261 cgcgttttgc gcagcagttg cacgtccacg ttgaaccagc gcggcgtctc ggacgtggcg
     4321 cgcgggtcgt aatagcggtg cgcaggatca aactgcgtcg catcgggata ggccgggctg
     4381 ctgacccggg ccaggccggc aatgccgggt tgtgcgcagc tggaatggta gaacagcaca
     4441 cggtcgtcaa cctgcatcag gtcgcgcatg aaattgcgcg cctgataatt gcgcacacca
     4501 taccatgcca cggtctgatt gggcaggcgg gcaagatcgt ctatactcac gtccgatggt
     4561 tcggacttca tcagccagta gcgcatgctg gaccatcgct gaaacgcaac cgctccattt
     4621 tactggaatt cgcgcactgg tgcgcaccgg acggaaattt tgaaagccca catgaagcaa
     4681 ctggataccc cgagcctcga ccggctggtc gaacaggcgc gcatgtcgcc acgcctgcgc
     4741 atgaatgcca acctgcatga acatctggac gatccggtgc agcgcctggc catcgcgatg
     4801 gagccgggca ctgtaatccg cccacaccgt cacgagcaca cctgggagct gctgctgccg
     4861 ttgcgcggac gtttcgtcgt gctgtggttt gacaattccg ggcatgttac cgagcgcgta
     4921 gtgctcggcg aaacctgcca ggcgcaggaa acgccggctg gagtgtggca cgccgtggcg
     4981 tcgctcgacg ccggcggcat cattttcgag gtcaagcagg gaccctaccg tccgtttgtc
     5041 gcgggtgact ttgcgccagg gtatgcgcat gacgatgcgg cccgtgacgg cgtgttgaac
     5101 gcctggtacc ggcaggcgca ggtgggcgat cgtctggcgt ggggtgcggc gggctgatgt
     5161 atcagcccgc gcgcgtcgtt caggcgtaag agcgcagggt gcgcgagaag gcggccagcg
     5221 actcgatacc gctggattcg gcgcgatggc accactcctg caactggtgc agcaattgtt
     5281 cgcgcgaggc attggagcgc gcccacagcg ccgagagttc ctggcgcatg gtgtacagat
     5341 gctgcagggt ctggctgcgc gtcaacactt cgttgaggcg ggcgcgatcg tcggcggtga
     5401 gttcgttctg gtcgaggtgc agccagcgcc tgagattacg tgccatgccg cggtcctcga
     5461 ggtgtggcag cgaggcgcgc aggcggtcga attcggtgcc gaacgaggat ttgaccatgc
     5521 gggcatagcg cgtgagcacg tcatagcggt gggtgatcac ggcctgtaac agatcgaggt
     5581 cgcataccgg cttggccggg ttgaagcgtg cgcgtggtgc gactttcttt accttggcca
     5641 gtcgagccat ctccatcagg cggatgtaca gccagccaat gtcaaactcg taccatttgc
     5701 tggatagcct ggctgaactg ccgaaggcat gatggttgtt gtgcagttct tcgccaccga
     5761 tgagaatgcc ccacggaatc aggttggtgc tggcgtcttc gcaggcgaag ttgcggtagc
     5821 cccagaagtg tccgagtccg ttgatcatgc cggcagcggt caccgggatc cagagcatct
     5881 gcaccgccca gatggtcagg ccgatcgggc cgaacagcgc caggtcgatg atcagcatca
     5941 acgaaatgcc gagcgtgctg tgcttctggt aaacgttgcg ttcgatccag tcgtcaggtg
     6001 cgcccgaacc gaatttctcc accagcgcct ggtcacgcgc cgcggtgcgg tacagttcgg
     6061 caccctcgag cagcactttg cggatgccga gtacctgcgg gctgtgcggg tcttcaggcg
     6121 tttcacagcg ggcatggtgt ttgcggtgca cggcagccca ctggcgtgtc accatgccgg
     6181 tggtcagcca gagccagaag cggaagaaat ggctgggcag cggatgcagt tcgagtgcgc
     6241 ggtgcgcctg gtgacggtgc aggaaaatgg tgacggatgc gatggtgatg tgggtcagcg
     6301 ccagcgccac caccacataa ccccaccacg gcagttcgcc tgccagaccg taggacagaa
     6361 aggatagcat cttgatggcc tcaagagata ccggtcagga ttgaccggag gggggatgaa
     6421 ttataccaga attcatcggg ttgtcgagat gttccgtaaa tacgtgcaca tcgcggcgcg
     6481 ggtaggggat gtcgatgcct tcggcggcga aacgttgcca gatggcgcgg ttgatgtcgg
     6541 aggtcaggcc gatctggccg agttccgggt caagaatcca taccagcaaa gccaggttga
     6601 tgccgctatc gccaaacgag gtcaggtagc tgttggcgtt gaatggttcg ttcttgagcg
     6661 tgcgcgggtg ggcggcgccg atttccgtga gaatggcgca ggcgcgttcc acgtcgctgt
     6721 cgtaaccgat ctgtaccgga atcttgacac tgacttcgcg ctgggtgaac gaatggttga
     6781 ccaccgttga tgtcatcagc gtttcgttgg gcaagatgat ctcggtgcca tcgaggacgc
     6841 gcagcagggt atagcgcgtg ttgagctggc gcacttcgcc ataatggccg tcgacggtaa
     6901 tcatgtcgcc tggccggatc gagcggtcga gcaggatgat gaaaccggac acatagttgc
     6961 tggccacttt ctgcaggcca agccccaggc cgacgccgag tgcgccgccg aaaaccgata
     7021 gtacggtgat gtcgatgccc actgcaggca gagcgatcag tacgctggtg atcagcagca
     7081 cagtgcgtac ggttttgatc agggcgagcc tcaggctggg attcatgcca ctggtgcgca
     7141 tcagtcgggc ttcgatcagt tgcgacagcc acagcgctgt gacgatggca agggtgacaa
     7201 cggcaatcgc ttccagcagc agcaacagcg agaagcgatg attgccggat tcgaaggcga
     7261 tgctgtcgag tacttcagcc atcaccggca gcagcccggt aatatgcaag gcgaagccgg
     7321 tccagatggt ccagacgatg acccggcccc agatgcggat gatgtcacgg ttgccggtca
     7381 ggtgccgcaa cagcaataca cccgtacgga tgagtatcag cgcgagcagc agcggaatcg
     7441 cgatgttgag tagatggact tcgtgatcgg gacgcaacag gtcacgtgcg aaatcggcga
     7501 gcagcagcca gcccagcggc agtccgatgg cgtggattat ccgccgacgc cgttccgggt
     7561 cggcaccgcc tgtcagcgag tggctggcga gcctgaccag tatccagcta agcgcaccgc
     7621 aggcgagcag ggcggccagt tgcagcagca gctcggtctg gtgcgattcg tagatcaggt
     7681 ggccgagcag gttttgcagt tgtcgatggg ggtgcatggc ggatgtcctt ccgcgtgttc
     7741 aggatcaggt tcgttccagc acggcggcaa agaaaccatc ggtgccgtgt tcggccggat
     7801 tgagcttgag gaaggggccg gtgtcgagcg cgataccctg ttcggcaagt acctcgctgg
     7861 ccggacgcag gtgaaattcg ccctgttggg caagaaacgc ctggaccacg tgttcgtttt
     7921 cttcctcaag caggctgcag gtagcgtaga ccagtcggcc gccgtgcttg accagtcgtg
     7981 ccgctgaagc caggatgtgc gcctgcttga tgctcagttc cgcgacgctg cgttcgttct
     8041 ggcgcacctt gagatccgga ttgcgccgca aggtccccag cccgctgcag ggcgcatcca
     8101 ccagtacccg gtcaatcttg ccggccaggc gcttgatgcg ggtatcattt tcgctcgtga
     8161 tccactgcgg cgtgatgttg gacagtccgg agcgtttgag gcgtgg
//
LOCUS       DAIANL010000050         3878 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2293661, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000050 DAIANL010000000
VERSION     DAIANL010000050.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2293661"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          815 a         1217 c         1076 g          770 t
ORIGIN      
        1 gcgaaacggg caaaacgcgc atactggtcc ggggtgtggc tgctgctgac gacctcgatg
       61 ccttcgccac cggcatcgcg gaattcatgc agcagcccga gcatgcgttc gctgcccacg
      121 cgatagcgcc ccggatgcgc cagcaccgcg cgtccgccgc tggcacgtat ccagccgatg
      181 gcatcggcca gtcctgccca tcgatgcgac acatagccag gcttgtcctt gctcaggtaa
      241 cggcgaaaca cggcctgcac gtccttgcag gcgccgattt cgaccagata gcgtgcaaaa
      301 tgcatgcgac cgatcagttc gggattgccg gcatggcgcc acgcaccttc cagactgtcc
      361 ggtatgccaa agcgcgccag ttgcgccgcg atgcgctcgg cgcgttcacg cctgccgaga
      421 cgcagttgcg ccagtcccgc agccagctcc ggcgcctgtg gatcgatgcc cagcccgacc
      481 acgtgcagcg tctgtgcttc ccagctgacc gagatctcca ctccgttgac aagacgcacc
      541 ccaagcgcct gcgcagcctg acgcgcttca tcgagaccgc tggtatcgtc atggtcggtc
      601 agcgccagca cgtccacgcc gcgttcggcg gcacgagcca gcaactgtgc aggggaaagc
      661 acgccatcgg agatgcagga atgggaatgt aaatcgtaat tgagcgacat ggcgtgtcag
      721 gctgattcaa ttgtcagatc ataacaaact tgccccccca cgtacataag gcacgccgtc
      781 agcccatgta cggctacgtg acatgcgatc atgtcaggaa acggaatttc cgttaaactt
      841 cctccgcact gccttgtcca ttgcatgatg tgacacgctt gccaacaccc atactgacct
      901 acaaccgaca tcatgaaatt ctttttcgac ctgctgcccg tcctgctctt cttcgtcgcc
      961 tacaaatggt tcggcatcta cgttgccacc ggcgtggcca tcgccgccag tctggcgcag
     1021 atcgtatggc tgctggcccg gggcaggaag gtcgatgcca tgctctggct cagtctggcg
     1081 gtgattgtcg tgctcggcgg cgccaccctg atctcgcaca acgagacgtt catcaaatgg
     1141 aaactgactg cgctctactg gctgtacgca ctgatcctgc tcgggggcga actctggttc
     1201 ggccgcaatt ttctgcgtat gctgctggga aaacagatgg tgctgccgga tgccatctgg
     1261 cgcagactga acctgagctg ggttgtgttc ttcgccgcca tgggcgggct caatctgtat
     1321 gttgcctacc acttcagcac ggatacctgg gtcaacttca aactgtttgg cagcatggcg
     1381 ctcatgctgc tgttcgtcgt ggcacagggc atcatgctga gccgccatct cggagatccg
     1441 cccaatgagt aactggtatg tcatcctcgg cgaagatgtt cccgacagcc tgcccatgcg
     1501 catgcagaca cgcgccgcac atctcgagcg catgcgccgc ctgcaggatg agggccgggt
     1561 gctggttgcc ggtccgtttc ctgccattga cagccctgat cctggccccg cgggcttcat
     1621 gggcagtctg attgtggcgc ggtttgattc gcttgacgcg gcacgtgcct gggcagacga
     1681 tgatccctac gtcagaagcg gggtttgggg gcgtgtcgaa gtaaaacctt ttgtgaaagt
     1741 catgccataa tgcacacctt ggaactaatg cgcgaaaaac tggccctatt gcaaccggaa
     1801 tcgctggaaa tcattgatga aagcgagcgc catgcgggcc atgccggagc gaaatcgggt
     1861 ggcgggcatt atcgcctgaa tatcgtttcg ccggtttttt gcgggatggg cacgctggaa
     1921 agacatcggc atgtgtatgc tgcgctgggt gaactgatgc gtgaacggat tcacgcgctg
     1981 gcgatccacg cgcactgccc atctgaaata tccggacgcg gcatttgagg cggtgccggc
     2041 tataacgaaa attccgtttc ataacttgta gacaaaggaa agttcatgca aatcaagaaa
     2101 cttgtcgtcg ccaccgcctg catcgccggt ctgaccgcgc tgaccggctg taacgccgcc
     2161 gaagacaaca agcctgccag tgccgcagcc ccggcgcaac cggccggcaa gatcatggcc
     2221 gtggtcaatg gtgtgccgat tccgcaggaa gagctcaatg tactcgagca ggatcgctcg
     2281 gcacagggtc agccgatcaa cgaccaggcc atcgctgcca tgcgcgacag tctgatcaac
     2341 ggcgagattc ttgcccagca ggcgcagaaa aaaggcatcg acaagaccag caatttccag
     2401 atgcacctgc aactcgccaa aacgcagatg ctggcccagg cattcattgc cgactacgtg
     2461 cgcgccaacc cggtcagcga agacgcgatg aaggccgaat acaatcgcgt caaggcaatg
     2521 atgggcacca aggaatacga agtcagccac attcttgtgg acaacgaagc cgaagcgcgc
     2581 tccatcatcg cccagctgaa caaaaaggcc aagttctccg aactggcgaa gaaagattcc
     2641 aaggacgcct ccgcatccaa tggtggacag ctcggctgga ttgccccggc aaatctggtc
     2701 aaggaattcg ccgatgccat ggtgcagctg aaaaagggtg aatacaccaa ggaacccgtc
     2761 cacaccaagt acggctggca catcatcaag gttgacgaca tccgtgacct gaaattcccg
     2821 gcctacgatc aggtgaaaaa ccagctgcgc accgatctgg aacagcagga gattcgcaag
     2881 ctgatttccg atctgcgcgc cagcgccaag atcgagtaat gccatagggg ttcccgacat
     2941 gtcgggaacc ccttcgattt ccacgacgga tcttcattca tgaaaaacct gcaagatcaa
     3001 tttctgaacg cgctgcgcaa ggagcatgtg ctggtatccg tgtttctggt caatggcatc
     3061 aagctggttg gcaagatcga atccttcgac cagtacatgg tcatgctcaa ggggcacgat
     3121 aacgtcagtc aggccatttt caagcacgcc atctccaccc tgtctccttc ccgtcaggtc
     3181 aatatcgacc tgggctccga agaatgacct acccgcgtac aggctcatgt cgcgcaagcg
     3241 cggcacaggc ccgtccgcct tcccgctcat gcgcggccga gcgcacggcc agatccgcat
     3301 tccgctggct gctcattgcg ctgctgctgt ttcagttgtt cgcgctcacc caccataatc
     3361 acgacatcag tgctcacgct gacgattgcc cgtcctgtgt tctggcgttg cacttttcca
     3421 gcagcggcac cactacccca cctgccctgc catcgcaatg gctgaccgtc ggttggccga
     3481 taccgcatca cgcccttgcc gtcaccccgt tcaccccacc tgctgcattt ctgctcccgc
     3541 ctgccctggc accgcctgtt cccgcacatt cccagtgagt cctgtgcgca ccgacatgcg
     3601 cagcaacacc gtattctttg gagcaaccga tgtcaggcaa accgtttgcc gggctggccg
     3661 cgctgtgcgc gctggcgagc ttttccgcgc atgctgcgga aattccggcg ggagaaatcg
     3721 atggcgtggt caacgatgcc ctgggtcaac ccctcgctgc agcacgtatt gaagtcaaga
     3781 accgcaacgg cgccgtgctg gcgatcacgc acagtgacag caacgggcac ttccatttca
     3841 cgcaccttgc ctcgggcgtc tatgcgctga tcgccacc
//
LOCUS       DAIANL010000051         9663 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_48911, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000051 DAIANL010000000
VERSION     DAIANL010000051.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48911"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2110 a         3253 c         2695 g         1605 t
ORIGIN      
        1 gcttttcgct ccagaccttg gtgttccaga tacgcggaat catgtgatag agcgaaccga
       61 agctcaccat ggcaacccag cccagcgcac cggagtgaac gtgtccaacc gtccagtcgg
      121 tatagtgcga cagcgcattg acatccttca gcgccatcag cgggccttca aacgtggaca
      181 tgccgtagaa cgacaaggcc acgatcagga aacgcatgat cggatcggtg cgcagacggt
      241 cccaggcacc cgacagggtc atgatgccgt tgatcatgcc gccccatgac ggcagcagca
      301 gcatgatgga gaacgtggca gccagcgtcg aagtccagtc aggcaatgca gtccagtgca
      361 ggtggtgcgc accaacccac atgtacagga aggacagcgc ccagaagtgc acgatcgaca
      421 ggcgatagga gtagatcgga cgaccagcct gtttcggcac gaagtaatac atcatcccca
      481 ggaatgcagc ggtcagaaag aaacccaccg cgttgtgacc gtaccaccat tgcgtcatcg
      541 cgtcctgggt gccggcgaac aggctgtagg acttcatcga gaacaggctg atggggacgg
      601 ccagggtatt gaaggtgtgc agcagggcag tcgccaggat gaacgacagg aagaaccagt
      661 tggccacgta aatgtgcggt tgtttccgct tcatgatggt gccgacgaac accgtcagat
      721 aaaccaccca ggtgatttcg atcaggatgt cgattggcca ttccatttcg gcatattcac
      781 ggctctgcgt gtagcccagg gtgtagctga tgtcaccgag cgcgatggca gcagtccagc
      841 cccagaacgt gaaattggcc aggaaatcgc ttaccagacg ggtctggcac gtgcgttgca
      901 cgatgtagta tgaggtggcg aacagtgcgc tgccgccaaa accgaaaatc acgctgctgg
      961 tgtgcaccgg acgaaaacgg ctgaaggtga tgtaagggat gtcgaagttg agaaagggcc
     1021 aggccagctc ggacgcaata tacacgccga cggacatgcc gagcaatccc catactactg
     1081 acatgaaggc gaatttcttc accacttcaa aattgtattg ctcattacta ccaatttctg
     1141 tagtcgtcac gtgagcctcc tatactgcta gtctgataaa ggatttatgc cagcgagcat
     1201 tctacctgcg gcaatatgtc gcacgcaagc ccgccaaaac gccatattat acgcaggttt
     1261 tctaacgctt tgataagcca tggataatca ggacacaaaa gtcctgtaat ttcgcccccc
     1321 tttaaatggc tatctcgatg atattatcca aataatggcc ggatgtctat tatcaatcca
     1381 ttttataggt catttgcttg cggtccgacc cgtttttttc gtcatgacac gacaacagcg
     1441 ctctgcgcaa gccatcgcaa cgcatcctga ttaacgataa atcaagtcaa tcgaccatgt
     1501 ctggccgatc acgcattcag tgactgccct gtttcacgct cagcagctgt tgcagatcat
     1561 gagtgatttg cggaaccgtt tccccgaacg gcatcagcag acgcaatcgg ccatccttgt
     1621 catacaaata ggcaaacgcg ctgtgatcca tgaaatagtt gccctgcgcg tcggcaggca
     1681 actggcgata gaatacatga tattcggcgg cagtccgggc agtggccgca gcatcaccgt
     1741 acaggccaac aaaggccggg ttgaatgccg gaacaaattc gcgcagcacc tgcggcgtat
     1801 cgcgacgcgg gtcgagcgtg acgaacagca cctgcacctg ctgcgcacgc acgcccagtg
     1861 atcgcatggc ctgcgccaga ttgtacagcg tcatcggaca gacttcgggg cagtgcgtgt
     1921 acccaaagaa catcaccaca accttgccac ggtagtccgc cagagtacgc ggttgaccgt
     1981 tcatgtcgtg cagtgaaaac tcccgcgcat acccggcgcc ggtgacatcc cgggcccagg
     2041 tctggcccag cggcacaggc ggcctggaac acgccgcgag cagcagcgcc agcagtggca
     2101 gcaggcgcag ccaggcagcc atcagggcgc cggcagcaac gcctgggtgg gcaggtcggt
     2161 cagacggtaa cgcccgcttt gcacggcagc agccagatca ccgagcttca tgttggtgag
     2221 catctcgatc acctgttcct tgatcggtag ccagctgttg tgcaccggac aggcggtatc
     2281 ttcgccgcag cgcttgaggc caagcaggca gctctgggta aaatcggcac cttcggtcag
     2341 ctgcagcaac tgcatcagcg acatctgcgc agtttcctcg cgcaggcaga aaccacccag
     2401 gcgaccacgg aaagaataca gcagcccgtt cttgccaaga ccctgcagga ttttggccag
     2461 atagggcgcg ggcacgttca gcctgagcgc aacgtcgcgg ttgagcaccg gcgtcccggg
     2521 cggttgggcc gccatgtaga tcatggcttg cacggcgtac tgactggtac gggatagaat
     2581 cacagcatca ccttcgatag agtatccagg aatatcccgg caaataccgc cgcgcccaac
     2641 cagttattat gcagaaaggc cttgaaacac gatgcaggat cgcgattgcg gataagtgca
     2701 taatgatata ccgcaattcc ggccgcaacg caaattccca gataaaaaaa catgccaaac
     2761 cggtaaatat aaccggcata cgccagcacg ccgagcatgg ccgcataaca gagcatgatt
     2821 gccgccacat caaaacgacc gaatgtgatc gccgacgtgc gaataccgat tttcaggtca
     2881 tcctcgcgat ccaccatcgc gtattcggta tcgtaggcca ccgtccagaa cacattggcc
     2941 acaagcagca accatgcctg gagaggcact gtcccctgga tggctgcata agccatcggt
     3001 atgccaaagc cgaaggccac accaaggtac gcctgtggaa tggcgaaaaa acgtttggta
     3061 tacggatagc tggccgccag cagcaccgcc ggcacggaca gccagcgcac cagcctgtcc
     3121 agcggcaaca cgagcaggaa cgcaagaccg gacagggccg tagccaacag caatgcctcc
     3181 cgcgtgctca cagtgcccgt ggcgagcgga cgatcgcgag tgcggctcac atgaccatcg
     3241 aaatcccggt cagcatagtc gttgatcacg cacccggccg atcgcatcag caccgtaccg
     3301 agcaggaaga tccacaccac gcgccagtcg ggaaaatgcc tgcccgctgc gagccacaac
     3361 gcccacagcg tcggccacag cagcagcaga atgccgatgg gccgatcaag ccgcatcagc
     3421 cgttcgtacg tatcgatcct tgcccacacc tgagccaact tcacgcgaat acatccggca
     3481 tgaatacctc cgtcaccaga atacgctgtc ttcccgagcc aaacaacgaa cgccgcgccc
     3541 acaggcgccc tgtcaccgcg gtaccggccg cggcctgtcg ccacagcgga tgcgtccgcg
     3601 gcagcagaca atactgcaac attccgcgac ggatgcgcgc atcggcgaac aacgcctcgc
     3661 ccaatggccg gttacccaga tgcgccagcc tgcgccacga gcctgacagg ctgtcctgtg
     3721 gcagcacgct gtgggcatat accagcggct gttctccgca gcacagccat acgttgcgaa
     3781 tcagcgcccg ccgtctcgcc gacaacccca ccggcctgcg ctcgtcagac tgcactgttg
     3841 cgcgccgcaa ctgcaaaccg cgtacgcaga acccggcgca acccgtgcgc agccgtcgcg
     3901 tcagcgaacg ccggtcggtc agccagtcgc gcacggctgg cggctcggcg atcaatctgt
     3961 tgtaccaatg tgccatgcca atccgtcggg ttgccacccg ctcacgcgag tccgtattcc
     4021 tgtcgtgcac caaaccagcg cgcgatatgc ccggccgcaa gcccgggcgt ctctcgcagc
     4081 atccagccgg ccgcctcacg cgctgattca accagcgcca catcagcgag cggatcggca
     4141 aaacgcagca gcggcgcacc tgactggcgc gcgcccagcc attcacccgg accacgcagc
     4201 cgcagatcct cgcgcgccac atgaaaaccg tcggtactgc cggcaatcac cctgagccgc
     4261 gcatcagcca gctccgacag cggttcacgg tagagcagca gacaggcgct gcgcgctgct
     4321 ccccgtccca cccggccgcg caactggtgc agctgtgcca gccccatgcg ctcggcgtgt
     4381 tcgatcacca tcagcgaggc attcggcaca tccacaccga cttcgatcac cgtggttgcc
     4441 accagcaggg cgatttcacc atgggcaaac gcggccatga cggaagcctt gtctgccgga
     4501 cgcatgcgcc catgcagcag gccgacgcgg aattgcggca gcatctgctg cagcatcgca
     4561 tgcgtgtcga tcgccgtctg caactgtagc gtctccgact cttcgatcaa cggacagacc
     4621 cagtacgcct gcgcacccgc ctggcagcgc gatcgcagcc agtcgaccac ctcgccacgc
     4681 cccccgtcgg caaccaggcg cgtgaccact ggcgtgcgcc ccggcggcag ttcatcgatc
     4741 agcgatacgt caagatcggc atgataactc atggcaagag tgcgcggtat cggcgtggcg
     4801 ctcatcatca gctgatgcgg catctgcccc ttgtggcgca gcgccaaccg ctgcgtcacg
     4861 ccaaaccggt gctgttcgtc gaccaccgcg agtccgagcc gggcaaaacc gacctcgtcc
     4921 tgaaacaggg cgtgcgttcc cgccaccagc tgcgccgtac cgtccgccaa cgcggcgacc
     4981 actgcagcac gccgccgcgc cgcaagactg cctgacagcc actgcacctg cacgcccatg
     5041 ggctcgaacc attcacacag cttgcggtaa tgctgttcgg ccaacagttc ggtcggcgcc
     5101 atgaacgcgg cctggaaccc acaatcgatc gcctgcgctg cggccagcgc ggcaacgatg
     5161 gttttgccac tgccgacatc gccctgcagc agccgctgca ttggcaccgg ccgcgccaga
     5221 tcgtcgcgaa tttcctgcca cacgcgttgc tgcgccgcag tcagcgcgaa cggcagccgc
     5281 tgcagcagcg ttctggacaa cgcgcccccc ccaggcatcg gagctgttcc cagccgggtg
     5341 cggcgcattc gcgcctgatg cagcaccagc tgctgcgcca gcagttcatc aaaacagatg
     5401 cgccgccagg ccggcgcact cttgtcctgc gcctgttgcg gcagggtggc cggtcctggc
     5461 cgatgcagcg ccagcacact ttcggcaaac ccggcaagcc cataacgttc gcgcaccggc
     5521 tcaggcaccg tatcgtccca gtccggatgc atcagcaacc ggtctgtcag cctgcggatc
     5581 atggtctgac tgaccccggc acacacaggg tagaccggcg tcaggcaatc cggcagcggc
     5641 gtgtcctcgg tcaccacccg ccagcgcgga tggaccattt ccacttccag caggcccgaa
     5701 cgcacttcgc cactgacccg caacagattt cccaccgcca tgcgctgcgc ctgtccggga
     5761 tacacatgca gaaatctcaa taccacccga ccgcaatcat cctcgatcgt cacctgcaac
     5821 tgacggcggc cacggtaggt cgtgtcacaa cggatgacac gaccttgcac ctgttgtacc
     5881 agaccaggcc gcagatcacg catcggcgtc aggtgcgtct catcttcatg tcgcaacggg
     5941 aaatgcagca acagatcggc tgcaatgtgc agccccagac gggtaaacgg ctttggcagc
     6001 ggtgccagca ggtccggccc gttggcagac acgccggaca cactcagtcc ttgccaggca
     6061 ccagcaggat cccgtcggct tcgacttcgg caccgcgcgg tagcgcggcc acaccgactg
     6121 cggcacgcgc cgggtagggt tcgctgaagt attgcatcat gatttcgttg accttggcga
     6181 aatgcgacag gtcaaccaga tagatgttca gcttgaccat atcatccagg gatgcgcctg
     6241 ccgcctgcgc caccgcgtgc agattacgga acacctggtg aatctgtccg gccacgccgg
     6301 attccagctg catggttttc ggattgaggc cgatctggcc agacatgtaa accgtatctc
     6361 ccgcacgcac cgcctgggaa tagctgccga tggccgcggg cgcatcaccg gtgctgataa
     6421 tctgtctcga catgctctgc tccgtcgtca aaatgtttcc gacccgcgag tatactatag
     6481 agcaccggca tcacgatcaa caccagcacc ggctgtactg ccagcccgga caccaccgcc
     6541 accgccagcg gctgctgcat gctggcgccc cggccaagcg ccagtgcaag cggcatcagt
     6601 gtcagcatgg cggcaaaacc ggtcatggcg atgggacgca tgcggttgcg gcccgccatc
     6661 accagccgcg catgcaggtc ggccgttttt tcaagctcga aaaattcgga gaaatagaaa
     6721 atggccgcct cggtgacgat gccgacgatc atggtcatgc ccatcatcga cgaaatatcc
     6781 agttccatac cgctcagttg caatccggcg aacacggcga acaccgacag caacggcatc
     6841 agcaagatgg acagcactac gctgaaacgt tcgtacagca gcaacagcaa cagaaacacc
     6901 agcccgatgg cggcaacaaa caccaccacc agcccgcgaa aagcgatctg ctgctgttcg
     6961 tacaagccgc ccagttcgac atacatgccc gacggcaggt tgccgggcgc agcgatggcg
     7021 cggcgcacat cggccatcac cgcccccatc gaccggccgc tgatgcgacc ggtcacggcc
     7081 accatgcgct tgaggttttc ccgcacgatc tgtgcctggc cactgacagc ttcgaccttc
     7141 gccacccgcc cgagcggaaa cagatgtccg tcaggcgcac gcagcatcag ccgactcaca
     7201 tcccgatcgc gctgacgcag accttgcggc atccagacgc gtacgccgat cagcttcggt
     7261 ccatgctgga tcgaagtggc gaccatcccg cccagatcgg tcgccaccgc ctgggtgatg
     7321 ccatccggcg tcatgccctc cagcgctgcg agcgcccgat cgatatgaat ctccagcgca
     7381 tcgccggccg gatggatgcc gtcacgggta tcgaccacac caggaatgcg gccaatccgt
     7441 tgctgcatcc gttgtgctgt ctgcagcaac gcgcgcgcat cgtcaccaaa aatcttgatt
     7501 tcaatcggct gtggcaccgc ggtcagatcg ccgatctcgt cctccatcaa ttgcgcaagc
     7561 tcgatgtcaa gtccgggaac ttcatgctcg atgcgggtgc ggatgcggtc catgaccaca
     7621 tcgatcggcg cacgctgacc gttacgcaga cgcacgaaga aatcgccctc gttcgcctcg
     7681 gtgatgcctc cgcccagctg ggcaccggta cggcgcgaat aggtctgtac atccgggtcg
     7741 gtcttgagga tcgtttccac ctggcgcaac aaccgatccg tctcggtcag cgatgtgcca
     7801 ggcgcagaca ggtagtccag cgtgaagccg ccctcatcca tcgatggcat gaatcccgag
     7861 cccaggttga caaacgccag cgtaccgccc atcaccagtg gcgcaagcac cagcagcagc
     7921 cacagcggcc gtgccagcag acggcgcatg aaggcgtcat agcgcagttg caacaggtgc
     7981 gtgaaacggc cgtcttcgcc ggcatcacgg ccgcgcaaca actgttcggc cagcaccggc
     8041 gcccccatcc aggtgacgaa gaacgaaatc agcagacccg ccgccatggt cagcgacagc
     8101 gccttgaaaa aggcgccagt caccccggtc aggaatgcca gcggcacaaa aatgatgatc
     8161 gtggccgcgg atgacccggc cagcgggcgc acgaactcgg ctgctgcctg caggatgcgc
     8221 tcatgctgcg cgccagtggt gccgcgcaaa cggcgcacga tatgctcggt catcacgatc
     8281 gcatcatcga tgatcagccc gaccgctgcc gccatgcctc ccagcgtcat gatgttgaaa
     8341 ctcatgcccg tcacatacag caccagcact gtcgatgcca gcaccaccgg cacgatgacc
     8401 aacgcgataa atgtaatccg caggttgcgc agaaacaacc acaacaccag aatggccagc
     8461 ccggtaccgg tcagcatggc atcacgcacg ctccgcgccg agtcggtcac cagcacactc
     8521 tgatcatacc agttggacag gtggaccccc gccggcatct gcgaccggta gcccgccagc
     8581 cgcgtggcca cgtcgtgtgc gatctgcaca ctgttgccgc ccggctgctg gaagatgttg
     8641 agcagcacgg cctgccgccc gtccgcattg acgcgagtcc agtgcggggc cacgccatcg
     8701 ctgacccggg ccagcttgcc cagcggaatc accgccccgc ccggggtgcg taccggcatg
     8761 tcacgcaatt gctgcaccgt ctgcagacgg gtatcagcca cggccagata cagcttgtag
     8821 tggtcttcaa gcctgccgac cgcatccacc acattgccag ccgtgaccgc ctgcaccacg
     8881 tcgcccgggg tcagccccca ggccgccagc cgcgccggat cgacttcgac ctcatactcc
     8941 tcacgctcac cgccctgcac ttccacgcgc gagaccccat ttacgctcga cagcagcggt
     9001 cgcaactggt actgcgccag atcatacagc tgactctggc tcagcgacga ggctgtcagg
     9061 ctgtaggcca gaatcggaaa caccgtcgga tccatgcgcc gcacggtcat ctgcgtgccg
     9121 ctcggcaact ggggcaacac ctgggtgatg gcctgaccga gctggacggt cgccagcgcc
     9181 atgtccatcc cccagtcgaa actcaccgag atttccgctt cgccccgact tgtagtcgaa
     9241 cgtacgttgc gtacaccagg taccaggcgc actgcctctt ccactggacg ggtaaccatc
     9301 accatcatca tgtcagccgg acggtcgccg gcatccagcg taaccaccac ccgcggaaag
     9361 tcgacattgg gaaacaacgt cactggcaag cgaaggctgg cagacagccc cgccagcgta
     9421 agtatcgcca gcagaaacag gatggagcgt cggtgcagct gcagccagcc ggtcacgttc
     9481 atggcgcatc ctggcgcacg gccatgccat tctgcagctc atagttgccg agcgccacca
     9541 ccggagcatc aggcagcagc tttccgctca ccgccaccac atcgccagac tcgatgccgc
     9601 gctgcaccgc cacacgccat gcccggccgc cccgcacctg gtagatgcag tcaccgttgc
     9661 cgt
//
LOCUS       DAIANL010000052        15661 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_50132, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000052 DAIANL010000000
VERSION     DAIANL010000052.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 15661)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15661)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15661
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50132"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3282 a         5166 c         4456 g         2757 t
ORIGIN      
        1 acacctgcat gttcatgatg gtggtatagg ccgataccag ccggtcacgc acctgaaccg
       61 cttcctgaaa actgatgttg gctttctgca tggcgatcat ggtctgcgac aaactgacac
      121 tgtcatcgcc agcggcgaaa cgttcaccca ggctatcggc ctgctgctgc acctgattca
      181 cctgatcgag cgaattcttc agcacctcgg caaatccggg gcccgacgcc gccgtggtac
      241 tggcggcagc ggtcgccgac agcccgctca acgcctgcgg aggctgcgtc atgttggccg
      301 cagcgcgaat ctgcgccagc atggctgcga tctgtcctgc atcgattccg ttcattccca
      361 tttgcgcacc tgtgcggctt tttcacgctt cacatgataa ttgcacgata gcaaattgaa
      421 ttcgaagaac agaaatccct ttttccctgt attcggctga tgacacgcgg caacaaatgt
      481 ggataattca cccatgggcg tcactccgtc gccccgccgt cgatggaatc gtacaggttt
      541 gacaacataa tatggccact gccggacaag acactgctgc acaacctgaa tggatgcaat
      601 tcatgcgttc gaccatcggc cagcgcatga tgcttgcgat cggcgctgcg gccgtgctgg
      661 ccatcatggt cgcggtgtgg atgtggagcc aacggccgga ctatcgcgtg ctgttttcca
      721 atttcagcga caaggacggt ggcgccatcg tcgctgtgct gcagcagatg aacgtgccat
      781 acaaatattc cgagggcggc ggcgcaattc tggtaccggc cgaccaggtg catgatctgc
      841 gcctcaaact ggcggcacag gatcttccca agggcggaaa cgtcggtttc gaggtcatgg
      901 aaaaccagaa actcggcaca tcccagtttc ttgaacaggt caattaccag cgcgcgctcg
      961 aaggcgaact ggccagcacc atcgaatcga tcaacgccgt gcgtgcggcg cgggtacacc
     1021 tggccatgcc caaggcctcg gtattcgtgc gcgatcagca gaaacccacg gcttcggtct
     1081 taatcgacct gcaacccggc cgcgcgctcg atcccggaca ggtcagcgcc atcgttcacc
     1141 tgctggccag cagcgtgcca gacctgaccc cggacaacgt gaccatcgtc gaccagaacg
     1201 gcaacctgct gtccgacacc gcaggcaaac acgaccggaa cggcctcgat ccgaaccagc
     1261 tcaaatacgt gcaggacatg cagcaggaca tcggcagtcg catcgaatcc atcatcaccc
     1321 ccatcgtcgg caaaggcaac gtgcatgccg aagtcaccgc cgacatcgat ttttccaaca
     1381 gcgaacaggc ggccgagacg tacaaaccca accccacacc ggacacctcg tcggtgcgca
     1441 gccagcagac aagcgaaacc agtgccgcgg ccaacccgcc aggaggcatt cccggggcgc
     1501 tcagcaatca gcccccggcc cctgcgacag caccgatcgc cgccgcgccg aacgcgcagc
     1561 ccggggcgcc cggagccgcc gccgcgccgc cagcccaggc acagatgatc ccggtgcaga
     1621 aagactcgac cacccattat gaactggaca aaaccatacg ctacacgcaa ctgccgatgg
     1681 gaggcattcg ccgcctgtcg gtagccgtgg tcgtcaatta ccgcacggtg accgacaaat
     1741 ccggcaaatc gcacagcgtt gcgctcacac ccgccgaaaa gacgcagatc accgacctgg
     1801 cccgggaagc catgggttat gaccagaacc gtggcgacac gctcaacgtc gtcaacacac
     1861 cgttcgtcgc gcctgcaagc gtgaacctgc cggaaacccc ctggtggaag cgcccggaaa
     1921 ccatcgaaat ggccaagacc attggccgtt acctgctggg cgccatcgcg ctgctcattc
     1981 tctatcgcaa attcctgcgc cccattctgg atggcctgct acacccgcct gcaccgcatc
     2041 acgttccggg aagcgaggca gaggaagata tcgaaggcag cgaagtccac ctgtcccatc
     2101 aggcggaatc ccgcgccgca gccgagcatc gttaccagaa ccagcttgag aacattcgcc
     2161 agatggcacg tgacaacccc aaagtgatcg ccaatgtcat caagggctgg gtcaataatg
     2221 aataacgaga agattcacaa ggcagccata ctcatgctgg cgcttggaga aacggaagcc
     2281 gctgaagtca tgaagcacct cggcccacgc gacgtgcaaa aactgggcgc agccatgtcg
     2341 accatggaac cgatcgcgca cgaagacatt tccaatgtgc tcggcgaatt caacacgatt
     2401 accggccaac attctgaata tggtaccaat tccgatgaat atctgcgttc ggtactcacc
     2461 caggccctgg gcaccgacaa ggcggcctcg ctgctcaacc gcattctcgg cgacgccgat
     2521 gtcagcggca tcgagagtct gaaatggatg gaccctgcat cggtcgccga tctgatccag
     2581 aacgaacacc cgcagatcat cgcgaccatc ctggtacacc tcgaacacga tcagtctgca
     2641 gacatcctgt cgaaattcac tgaacggtta cgtaacgatg tgctgctgcg catcgccacg
     2701 ctcgacggcg tgcagccggt cgcgctgcag gaactcaacc aggtactgac ccggctgctg
     2761 tcgggcaacg aaagcaacgc catcaagaaa caggcgatgg gcggcacccg gactgccgct
     2821 gaaatcctca acttcatggg cggcaccatt gaaaattccg tcatggacgc cttccgcaac
     2881 tacgatgccg acatggcgca gaaggtcatg gacgcgatgt tcggtttcga gaacctgatg
     2941 gatatcgacg accggggaat ccagctgttg ctgcgagagg tgcagtccga ctcgctgatc
     3001 atcgcgctca agggtgcgcc gacagaactc aaggaaaaat tcctcaagaa catgtcgcag
     3061 cgcgctgccg acatgctgcg cgaagatatg gaaactaaag ggccggtgcg cgtctcggaa
     3121 gtcgaagcgc aacagaaagc cattctggtc atcgtgcgcc gactggctga cgaagggcag
     3181 ctcgttatcg gtggcaaggg cgatgatgcc ttcctgtaac cggcgacctg ccctcatgaa
     3241 cggacatatg ccatgattcc caaggaacag caaacagcct ggcaacgctg ggaactggga
     3301 tcgctcgaca ccgcagcgcc aaagaaacct ggcgcagccg gcgtacagcc tcccaccgta
     3361 aacgaactgg aacagttgcg cgagcaggca cgcagggaag gttacgccgc cgggctggcg
     3421 gaaggccggg aggccggtca tgccgcggga ttgcgggatg gcatgcaggc aggactgcaa
     3481 caggctgcgc aacagaatga ggcattggca cagcagtggc aggcactgct ggatggattc
     3541 gctctcgaac tcgctcaggc cgaccagcac gtatcggccg aactgctcgc actggcatgc
     3601 gatctcgccg ccagcatgat ccggaccgtg cttccggtga aacgggagct ggtgctgcca
     3661 ctggtcgaac aggccatcgc agccctgcct gcgatccagc gtggcggcaa gatccacctg
     3721 aatcctgcag acgcagcgct ggtccgtgaa cacctgcatg acgcactggt acagcaaggc
     3781 tggcatctgg tggaaaacat ccagatcgcc gcaggcggct gccgtatcga gacgcacagc
     3841 aatcaggtcg atgccacgct ggccacacgc tgggaacgct tgatgcgtgc acttggacag
     3901 gacacggcat ggatcgcccc ctgaccccgc cagacacgca tggcagcacg cgctggaagg
     3961 ccagcctggc cacctgcagc gcggcgctgc ccggcatcga accgatggaa atttccggac
     4021 gcatcacccg ggtcaccgga ctggtgatcg aggctgtcgg cctcaagctg ccgatcggca
     4081 gtgcctgtgt cgtgccgcta cccagtggcg gacgcatcga agccgaagtg gtcggcttca
     4141 acaacgacca cctgttcctc atgccacaaa acgatgtgga aggcgtcatc cccggcgctg
     4201 cggtgctgcc gctggaaacc actccgccgc taagccctgg cgccacggtc atgccgcggc
     4261 gccgctccat cgaccgcacc cgacacctgc cagctggcga tgccctgctc ggccgcgtgc
     4321 tcgacggggc aggacggcct ctcgatgcac ttggcccgct gccacccatg gatagcacac
     4381 cgttggcggt acgcgccgcc aatccgctgc accgcgcacc aatcaccgag acgctcgatg
     4441 tcggcatccg caccatcaac gccctgttca ctgtcggccg tggtcagcgc atggggttgt
     4501 tcgccggctc aggcgtaggc aaaagcgttc ttctcggcat gatggccctc tacaccaatg
     4561 ccgaaatcat cgttgtcgga ctgatcgggg aacggggacg cgaggtcaag gaattcatcg
     4621 agcagattct cggtaccgaa ggactggcgc gcgccgtggt catcgccgct cccgccgatt
     4681 cgagcgcact gatgcggctg cagggcgcgg cctacgccac ctcgatcgcc gaatatttcc
     4741 gcaaccaggg ccgtcatgta ctgctcatca tggattcgct cacgcgctat gccatggcac
     4801 aacgcgaaat cgccctggcc atcggcgaac caccggcaac caaaggttac ccaccctcgg
     4861 tgttcgcccg cctgccagca ctggtcgaac gtgcaggcaa cggccgcccc ggcggcggat
     4921 cgatcactgc gttctacacc gtgctgaccg aaggcgacga ccagcaagac cccattgccg
     4981 actccgcgcg cgccattctc gacggccata tcgtgctcga ccgcaagttg gctgaatccg
     5041 gccattatcc cgctatcgac atcgaacagt cgatcagccg cgccatgcac aacatcacct
     5101 ccgccgagca ccagcaacag gcacgccgcc tgcgccaact ggtttcggcc tggcaacgca
     5161 accgtgacct gatcaacgtt ggcgcttatg tcaacggcag cgatccactg gtggacgaag
     5221 ccatcgcccg ccgggcacgc atcaacgcct tcctgcaaca aggcatcaac gaacaggcat
     5281 ccattccggg gagtttgggg caactttcgg ctctgttctc ctgattgaaa tccgtccgac
     5341 gcgctacaat gcaaatgaga ctccggatta tccggcgaat gtcgcctgct acagggaggc
     5401 aacacctcct gacaggtcat gcattcgccc agccaccact ccggcagttc catgatttaa
     5461 ggagaacggg cgcatgattc aaccagcggt tctggacacg ctcatcgaca tggcagacaa
     5521 gcaggccgaa aagcttgccc agcgggttgc cgccgcagcg cgtgcgcacg aagaacactc
     5581 gcagaaactc accctgctga tgcaataccg cgaggactat cttgcgcgtt accagcgggg
     5641 gctgtcgcac ggacttggcg tggcagacca tctcaatttt cgtgaattcc tgtacaagct
     5701 cgacgaagcc atcgacgggc agcagactct ggtcaaccag gccatcggcc gcctcgacca
     5761 ggaacgcagc caatggcagg aagcggagcg caagcggctt tcctacagca cgctttcgca
     5821 gcgcgcactg gcccagcagc aactggaaca ggccaggcgc gaacagaaag ccagtgacga
     5881 atatgccgcg cgcatgacac gaagcgccgg cgcatggcag agcgcaacga ccaaacccga
     5941 atgctgaacc tgcaaaaaag gaagccatca tgaccccagc gatccttccc gttatcgcgc
     6001 cgaatggcgg caatgcgaca accgacggca gcaagccgac ccagaccggt aatgacggcg
     6061 agtttggcaa catcctgcac caggagatcc gccagcaggc gcagcaatcg caacaatcgc
     6121 agcaatcgca acaatcgcag caggcgcaac aatcgcagca ggcacagccg gcgcaaacgc
     6181 catcgccatc cacggtgggg ggaaacaaaa gcccggattc gaacagcacc ccggatcaga
     6241 catccagctc cggcgtcagt ccggcaacag tggtcacacc atccactgcg ggcgagacgc
     6301 cagcgttcac cgcaagcagc gaactgcctg tcgccgccac gccggctgcg cagcagatgc
     6361 cagccggcac ccccccgacc gcgaccggcc tgcccgcacc ccgggacaac acaagcatgc
     6421 cagccgcccc gaacctgact gcggcaccac aatcggcggc gctggctcct gcgatcaagg
     6481 gaacgacctc caccactcag gcaggttctg ccggcgcggc gacaaaacag gattcccgga
     6541 aaacggacgt cagcgcgtct cagccgcaaa cggccaccgc agaaatgctg gcgctgatgg
     6601 gttacacccc ccccacccaa atggtgggac acacgcctgc aaccgataca gccaccgccc
     6661 cccgccagaa tgacgctgca tcggctgtac aaaccggcca gcagccgatc gtgtccagcg
     6721 ccaccagcga cagcaacacc atcccggggc tccctgccgc taccgcgcaa caggcagacg
     6781 ccagttttgc caaccagctc acagcacaac ttgccagcca gaatctgccc acggcgggcc
     6841 atggtgtcca cagtgccgcc gatcccgcag cactggccat cacccaggca ggccttggcc
     6901 aggccggcac tgctcaggca gccggagccg cgctgccatc ggcattcagc ggcacgcttg
     6961 cgccgcgcgt cggcaccgcg gaatgggacc agtcactgtc gcagcacatc gtctggatgg
     7021 cccacggcgc cgagcagacc gcttcgctca cgctcaaccc gccggatctc ggcccgttgc
     7081 gcatcgtgct caatgtgtcc aacaatcagg ccagcgctac cttcatcgca caacaaccgg
     7141 aagtccgcca ggcactggaa aacgcgctgc cccggctgga ggcaatgatg aacagcgccg
     7201 gcatccagct tggccagacc agcgtgcgcg ccgatacgcc aggccagcag gaccagtctg
     7261 cctggcaacg ctccgggtca tcgtccgcat cagccacccg cggaatcggc ggcgtcagcc
     7321 gtgcgggcgg actggttgcg accgaaaaga ccaccggcgg acaagggttg atcaacacat
     7381 tcgtgtaatt gcgattacaa tattgcggtc gcccaacctg actggagtgt ttcatgagca
     7441 aagcaccacc cgcccccaag gccgaaaccg aaactgcccc ggtagccggc aagtcgaagc
     7501 tgctgatcat cattatcggt ctgctggctg tcctgctgct gggtggagga ggcgcagctg
     7561 catggtactt cctgcatgca tctccggcac aggcgtccgg tgcgaagaag gccccggtgg
     7621 atgaggatgc cggcaagccg ccggtatttg tcgccctcga tccattcact gtcaacctcg
     7681 cacccactga tgacggcgac accaaatacc tgcagatcgc cattacggtc cagattcctg
     7741 acgacaaggc aggcgacaac ctcaaggcca acatgccact ggtgcgcagc cgcatcctgt
     7801 tgctgctgtc gagccagaag gcgtccgacc tgcttaccga agccggcaag gccgcgctca
     7861 tgaaagcgat cattgccgaa ctgaccaaac ctttcagccc ggatgccccc ccgcaaaaga
     7921 tcaagggggt atttttcact tctttcgtga tccagtaaaa acgacatggc agaaaatttc
     7981 ctgtcccagg atgaagtcga tgccctgctg cgcggcgtaa ccggcgagca ggacgtcgag
     8041 gaggcgcccg ccgatacctc gggtgtgcgt ccctacaacc tggctacaca ggaacgcatc
     8101 gtgcgcgggc gcatgcccac gctggagatc atcaacgagc gcttcgtccg cctgttccgc
     8161 atcggcctgt acagcttcat gcgccgcacg gcggaaatcg ccaccggccc ggtgcgggtg
     8221 cagaaataca gcgaattcat ccgcaacctg gtcgtgccga ccaacctgaa tctcgcgctc
     8281 atcaaacccc tgcgcggtac cgcactcatc gtgttcgatc cgacagtgat cttcatgatc
     8341 gtggacaacc tgttcggcgg cgacgggcgt tttcacaccc gcgtcgaagg ccgggatttc
     8401 acccagaccg aacagcgcat catcctgcgc gtcgtggata tctttttcga gaattacgcc
     8461 aagtcgtggt cgtacgtgta cccgatcgaa ttcgaatacg tgcgcagcga aatgaacacc
     8521 cagttcgcca acatcgccac gccgaacgaa gtagtcgtca gcaccacctt taccatcgaa
     8581 ctcggcgcct cgcatggcga atttcacatc tgcatgccgt attcgatgat cgaaccgatc
     8641 cgcgacctgc tcaccagcag cctgcaaggc gaaacgcttg atgtcgacaa acgctggacg
     8701 cgactgatga cgcagcagat ccagaatgcc gaggtcgaaa tcgtagccca catgggttcg
     8761 gcccagctca gcatgggcga cctggtcaac atgcaggtcg gcgatatcat cccgatcgat
     8821 attccggaaa ccattaccgc cacggtggat aatgttccgg tcatggaatg cagctatggt
     8881 acgctgcacg gccagtatgc actgcgggta gaaaaactga tctcgcatgc gatatccgaa
     8941 gtcacgccgc aaggagaaac atcatgagcg aaaccggact cgacagcagc gccatggacg
     9001 actggggtgc cgcgatggcc gagcaggccg ccgccgatgc cgttgcagac accagccagt
     9061 ccaggccggc cgtggaacct gccgcgccga cgttccagga gttttccagc agtagcggtc
     9121 ctaccggaat ccacaacgac attgatttca tcctcgacat tccggtgcaa ctcaccgtcg
     9181 aactcgggcg cacgaaaatt tccatcaaga acctgctgca gcttgcgcaa ggatcggtcg
     9241 tggaactggc gggaatggcc ggcgaaccca tggacatcct catcaacggc tacctgatcg
     9301 ctcagggcga ggtagtggtg gtcaacgaca agttcggcat ccgggtcacc gacatcatca
     9361 gcccggccga tcgcatccgg aagctcaaca agtaatggga cgactggcag cctttctttg
     9421 cggctgggcc gccttgcccg cctgcgccgg tacggcaccg ccgcacccgg cagacaccgt
     9481 tccgctgttg cagatcagcc tgagcctgct ggccgtactg ctggcgattt tcgccatcgc
     9541 ctggtttgcg cgacgctacc tggcgctggg cggacaggcc agtggtgcaa tccgcgtggt
     9601 cggcggcctg caggtcggca accgcgaacg ggtgctgctg ctcgaagtcg gcgaacagtg
     9661 gctggtgatc ggtgtggccc ctggccgcat cgatgcgctg gccacactgc cacgccagga
     9721 actgccctca tccgccactt ccggcgcacc cggcggattt gcagccagcc tggcggcccg
     9781 aatgcgcacc atcaagactc ctcatgatgc acactgaccg ccgtaccctg ctttgccgac
     9841 gcgcaaccgc acgcctgttg ctgatcggcg ccggtgcgct gctgccggcg ctggcgttgg
     9901 cagcaccacc ggcgctgtcc gcgcttacca gcaccccggc accgaacggt ggccagacct
     9961 ggtcgctcag catccagacg ctggtgtttc tcacctcgct gtcgttcctg ccagccgtcc
    10021 tgttgatgat gaccagtttc acacgcatca tcatcgtgct ctcgctactg cgccaggcgc
    10081 ttggcacaca aaccacgccg cccaatcagg tattgatcgg tctggccctg tttctcaccc
    10141 tgttcatcat gggcccggta tttgacaaga tatacagtga tgcctatcag ccgctggctg
    10201 ccgaccagat cagctttcag caggcactcg accgggccag tgtgcccatg aaggccttca
    10261 tgctgcgcca gacacgccag tccgacctcg cactatacgt gaagatgtcg aatacgccgg
    10321 ccttgagcgg gccgcaggat gtgccgatgc gcatcctgat cccggccttc gccaccagcg
    10381 aactcaagac cgcgttccag attggcttcg cgatcttcat accgtttctc atcatcgaca
    10441 tggtggttgc cagtgtgctg atgtcgatgg gcatgatgat ggtggcacct tcgatcgtgt
    10501 cgttgccatt caagatcatg ctgttcgtgc tggtcgatgg ctggcagttg ctgctgggct
    10561 cgctggcaca aagtttttac tgaggatgac gcatgactcc ggaaaccgtc atgaccatgg
    10621 gccgccacgc agtggaaatc atgctgctgg tttcggcacc cctgctgctg accgcactgg
    10681 tcatcggcct gatcatcagc atcttccagg ccgccacgca gatcaacgaa gccacgctat
    10741 cattcattcc gaaactggtc ggcatcctca ttgcactgat tgtcaccggg ccgtggatac
    10801 tgagcgtcat gctcgattac atgcgcgaaa tgctgaccac attgccatcg ctggccggct
    10861 agcccgccat caccgcattg aacccgccag tggagtctcc cggcatggtg cctgaatgcc
    10921 tgatcgcctg acatgatcca cttcaccgac acccagcttt atgcctggat cgccggcctg
    10981 gtgtggccac tttcgcgcat tctcggcctg atcagctccg caccggtatt cggtcaggcc
    11041 aacgtgcccc tgctgagcaa gcttggcctc ggcatagtgc tcacgctgct gctggcgccg
    11101 gtgctgccac ccatgccagc cattgatccg tcgtctcccg caggactgct gatcctcgcc
    11161 cagcaggtga tgatcggcct gtcgctcgga ttcatcatga acctggtgtt catggcaatc
    11221 gacatcgcgg gtagcctggc cgggctgacc atggggctgg ggtttgccag tttcttcgat
    11281 ccgcagaccg aggggcagac caatgctgtc aaccagttgc tggccctgct cgccaccctg
    11341 gtgttcctgc aactcaacgg ccatctggta ctgatttcag tgctggcgga cagtttccgc
    11401 catctgccgg tgggacaatg gccggaccgg tcggtggtgt ggcagatcct tgaatggagc
    11461 aagctgttgt tttcggccgg gatccagctg gcgttgccgg tggtcaccgc gctgctgatc
    11521 accaacgtcg cgcttggcat cctcacccgc gccgcgccgc agctcaacct gttcggcatc
    11581 ggttttcccg tcaccatcag catcggtttc atcactctgc tgctgtcgct gcagctgatg
    11641 ctgcatccca tggaagtgct gttcgacaag gcgctgcagc tgctctccag cctgacggca
    11701 cacacggtgg acatacgctg atgcatttgc gctgacccag gggtaccggg tgcagccggc
    11761 gcacgtctgc gcaggttcag ccatggcgca cgtctgcgcc ggattcaacc atggcgcacg
    11821 tctgcgccgg attcagccat ggcgcacgtc tgcacattgc gcccggtgac gcaatgcatg
    11881 atcgaccagt accagcgcag tcatggcctc tacgatcggt gtggcgcgaa tgcccacaca
    11941 cgggtcatgc cggccatggg tttccaccag caccggttcg ccggcacgat tgatcgaccg
    12001 gcgtggaata cggatgctgg aagtgggctt gatcgccacg ctggccagaa tgtcctgccc
    12061 gctcgaaatg cccccgagca cgccaccggc atggttggaa agaaaccctt ccggagtgat
    12121 ttcgtcgcca tgctcgctgc cgcgttgctc caccgcagcg aaacccgcgc cgatctccac
    12181 gcccttgacg gcattgatgc ccatgagcgc ccaggcgagg tcggcatcga gccgactgta
    12241 caccggttcg ccccaaccag ccggaacgcc ctcaaccacg acattgaccc gcgcaccaac
    12301 tgaatcgccg gcgcgacgca gatcgtccat gaactgttcc agcgccggca cactgtcggc
    12361 atcaggcgca aaaaacgcat tatccgacac tgccgcccag gatctgaacg gaatggcgat
    12421 ggggccaagc tgggcaaggt agccgcgcac ctcgacgtta tggcgctcgc gcagccattt
    12481 gcgcgctaca gccccggctg ccacgcgtac ggcagtctca cgcgcggaag aacgcccgcc
    12541 accacggtaa tcgcggatgc cgtacttgcg ccagtacgtg taatcggcat gtcccgggcg
    12601 gaacgtgtca acgagattgc cgtaatcctt gctgcgctgg tcttcattgt gaatcagcag
    12661 cgcgatcggg gttccggtgg tacggccctc gaagacgccg gagagaattt gcaccgtgtc
    12721 ggattcgcgt cgctgcgtga cgtgccgcga ggtacccggc ttgcgccggt cgagatcggc
    12781 ctgaatgtcc gcttcggaca gttccagtcc gggtggacat ccgtcaatca cgcagccgat
    12841 ggccggaccg tgcgattcgc caaacgaagt cacgcaaaac agtttgccaa atgtattccc
    12901 cgacatcctt cattcacccg tttcaatgct gattctggtt cattgttcct gcctgttgcc
    12961 attgcggcca acagccatcc cgttcatgct cgagcgcgct tgtcgcgcct ggggagcgcc
    13021 ggttcgtcaa gcaactgctc cagccactgc acgccccatg ccacgccgaa accggtcacg
    13081 tcactgccga cggctcccag cccgtccttg caattgcacg ccgacagatc catgtgcagc
    13141 cagggcagcc catcgacaaa acgcgccaga aaacacgccg ccagaatatg atcggcatca
    13201 ccttccaggg tgcactgctt cacgtccgcc actttcgact cgagcgcagt gtcgtaatcc
    13261 tcgtccatgg gaaatacgca cacgcgttca ccactcgttt cgccggcgga acgcgcatgc
    13321 cgcaacagct ccacatggtt gccaagcacg ccgctgtagc ggttgccgag cgcgctgacc
    13381 atgctgccgg tcagcgtggc gaaatccgcc aacacatccg gtcgttcgcg cgcggccagc
    13441 gtcagtgcgt cggccagcgc catgcgcccc tcggcatcgg tatgcacgat ttcaatagtg
    13501 gtgccgttga gtgcggtaac gatatcgttt tgccggtagg cctggggact gacatcgttg
    13561 cgtgcaatgg cgagccacac atcgagcgca atcggcagat cgagcgcact gacggcatga
    13621 aggatgccca gggcaacggc cgcaccgttc atgtcctcat gcatgccatg catgtggcgc
    13681 gccggcttga gattatgccc gccggtatcg aaacagatgc ccttgccgac cagcgccacc
    13741 cgcaggcgcg gctgcgggtg ctgccagccc agtcgcacaa tggctgcatc attgtcctgg
    13801 ctgccctggg caacggctac gaacgcacct gcgcccagct tgcgcagttt tttcaggtca
    13861 tattcctcga actgccagcc atgccgtccg gcaagcgtcg ccacctcgcg tcgcaactgt
    13921 gcaggcgcaa gccggttggg tggcagcacc gtcaggctgc ggcacaagac gttggcagcg
    13981 gcgatggcgc gcagccgttc cagccgggta cgcggcagtt cgccatagac ggtcactttc
    14041 ttcagtccgg gctgcgccgc cgaagtcttc tgttccggca gccgcgcacc gttgatccac
    14101 agtacatagg cagcatcggc cactgcgagc tcacgggttg tcgcatcgcc atgcacgttc
    14161 agcgcaatgc gctcgggcgc ttcctcgagc agtcgttgca ctgcagcacg caagcctgca
    14221 aggcgttcgt aggtggcgag ggtggcatcg aagcggatcc atactgtcag caccccctct
    14281 gccgacaccg tgcacaccgg cttgcgcagc atgtcctgcg cccgcatgcg ccgccgtgtg
    14341 agcacgcctt gcaaggcaac cgcctgctca tcgggcacgc tggcaagcca tgcggcatct
    14401 gcgggaagaa tggtcaacac gtgggacagg ctggcatccg cactgacgga aaacgccttt
    14461 ttggcgcgaa caaactgggc cagggccatg gaatcggtct gagtcatctc ttgagtcggc
    14521 aaaatgcgcg caacgaacag ggacaatggg tttcagccgg gcaaaaggcg tggttttgac
    14581 ctgccccccg gtttatcaga taataacaga gtttgtgcaa ccccattgag cctttggcaa
    14641 ggaagacttt catgactctg gacgacaaga agcgcgtgtt gcgggaaatg gtattgcatc
    14701 gccgctttga agaacgctgc tatcaagcct atatcgaacg caagatcggc ggcttcctgc
    14761 acctttatcc cggtcaggag gcctgctgca acggcgtgat ggaagccgct cgtcccggcc
    14821 atgactacgt catcaccggc taccgcgatc acatccacgc catcaaatgc ggcgcagatc
    14881 ccaaagccgt gatggccgag ctgtatggca aggagaccgg ctcatcgaag ggtcgtggtg
    14941 gctcgatgca tattttcgac gtcgcccacc gcttcatggg cggctatgca ctggtcggtg
    15001 gaccgttccc gctggcggcc ggtatcgcca aggccatcca gatgaaaggc ggcgacgaga
    15061 tctccatctg cttcctcggt gacgcagcca acaaccaggg ggtgttccac gaaacgctca
    15121 acatgtccaa actgtggaac ctgccggtac tgttcgtatg cgaaaacaac ctgtatggca
    15181 tcggcacggc gctggcacga tccaccgccg tacccgaaca gtacaagcgc gtatgttcct
    15241 atggcatcga atcggcccaa tgcgatggac aggatgtaga tgtcgtctac gaacatgcca
    15301 gaaccgcggt ggagcatgta cgcagcggca agggcccgtt ctttctggaa ctcatgacct
    15361 atcgctatcg cggccattcc atgtctgact cgcgcggtta tcgcagccgc gaagaagagg
    15421 aggaatggaa aaagcgtgac ccgctgatca tcttgcgtga ccggctcatc gccgccggcg
    15481 cattgacggc ggaagaatac cagggcatcg aatccacaac cgacgcctac atcgaaaatg
    15541 aagtcatcgc ctttgcggaa tcctctcccg agccctccgt agccgccctg gaaaaatacg
    15601 tgctcgcctg accgcgaccc cgcctgcggc caacggctgc ccgcggcgga ctatcgaatc
    15661 t
//
LOCUS       DAIANL010000053         9082 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_52106, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000053 DAIANL010000000
VERSION     DAIANL010000053.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9082)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52106"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1970 a         2439 c         2887 g         1786 t
ORIGIN      
        1 tcaatgccag cagcgtggcg ggcagttcgc ttagccagtt gcaggcgctc gatacggtcg
       61 gatcgaacct cgatgtacaa tacagccaga ctctgtcaaa cctgcagagt ctggattacg
      121 ccaaaaccat ctcccagctc agtgagcaga ccaccacgct gcaagcggcg caaatatctt
      181 ttgtaaaaat agagaatttg tcgcttttca attacataaa ttgagataag atagaagccc
      241 gggctatgtg ccggcgtgcg cgcatgcgcg gccacgcggt gcggttgtgt ggacgacgga
      301 agaagcgtct tcgggctgtg tccgcgacag tacaatgcgg gtgacgtgct gtcgtgggcg
      361 cgattttgcg agcggcgcgc ctacggccat cgcattgttt gccgcgtcgt caccagaatt
      421 gatgcaggca ggggaggctt gaggggtgat agatgatcga tgacttgagt cagtttcatc
      481 aggttttttt tgatgaagcg actgaattgc tggccgaaat ggagcgtctg ttgctcgaag
      541 tcgatgtggc cagtccgtcg caggaagaat tgaacgcgat tttccgcgcc gctcattcca
      601 tcaagggcgg agcggccact ttcggattta ccgacatcag cgaatttacc catgtgctgg
      661 agtctttgct ggacaagatc cgcaagggcg aaatggcgct gactgccgag catgtcgata
      721 catttcttgc agccaaggac gtcatggcca tgttgcttga agggcatcgc gccggcggcg
      781 ccgttgacct ggatcaggtg gccgaagtca gccgcaggct gcatgcgctt gcggatcctg
      841 cagccggaaa acaggcggcc gcgccgttag ctgcagcggc gcccgaaccc gggccagaac
      901 ctgttgccca accggctaca accgtagccc cggcttctgg cgcgctggca atattccgta
      961 tcgaactgcc ggtgacgcca gacaaggacg ttgaaggtct ggccgccgaa ctggggttgc
     1021 tgggtacgct gacgcgagag ccgctgccgg acggccgcat ggtgtttcat cttgaaaccg
     1081 accagaccca gagtgacgtt acagcagtgt gttcgttcgt cgttgacgca gacaagttgc
     1141 ttattacggc acgggaacca gcggtcgcgg cagccgcgtc cgtggccgtt gtggctgaac
     1201 ctgccgccgt gccggttgct gctgcggcag cagcggtgcc ggtgacagcc tctcaggccg
     1261 atgccgcagt caaggccgcc aaacgcgagg ccggtgcacc ggcagggcac gaatcgagct
     1321 cgattcgcgt gagcatcgag aaggtcgacc tgctgatcaa tctggtgggt gaactggtca
     1381 tcacccaggc gatgatcgat cagcgcgtga gcaccctcga tccggtaaat cacgaggcat
     1441 tgctgaacaa tttcgcccaa ctggcacgca acacacggga tttgcaggaa tcggtgatgt
     1501 cgatccgcat gatccagatg gattatgtgt tctcgcgctt tccgcgcatg gtacgcgaac
     1561 tggccagcaa actcggcaag aaggtcgatt tcatcactca gggggcctcg accgaactcg
     1621 acaagggatt gatcgaacgt atcatcgacc cgttgacgca tctggtgcgc aacagcatcg
     1681 accacgggtt cgaactgcct gcagagcggc tccaggccgg caaacccgag gccggcaagc
     1741 tggtgttgtc tgcttcgcat caaggtggca acatcgtcat cgaggtcatt gacgacgggc
     1801 ggggtctcaa ccgtgcgcgc atccttgaca aggcgcgaca gaacggactt ccagtcagcg
     1861 acaacatgac cgacgaggac gtctggcagc tgattttcat gcccggcttc tccactgccg
     1921 atgtggtgac ggatgtttcg ggacgcggtg tcggcatgga tgtggtgcgt cgcaatatca
     1981 ccgatatggg cggtaccgtg gaagtgcgtt cggtggcagg gcaggggtct tcggtgcgga
     2041 tttcggtgcc gctcacgctg gccatccttg atggcatgtc ggtcaaggtg ggcaccgaag
     2101 tatacatcgt gccgctggcc tacgtggtcg aatcgttgca gccgggcgcc agcgaactga
     2161 aggaaatggc cggtcagggg cgtctgatca aggtacgcga cgagtacctg ccgctggtgt
     2221 acctgtatga acgtttcggt atcgtgccaa agtatgccga gcccaccgaa agcatcgtgc
     2281 tggtgctcgg agtggacgac aagaaattcg ccttgctggt cgacacgctg gtgggccagc
     2341 aacaggtcgt ggtgaagaat ctggaaacga atttccgcaa ggtgcccggt atttctggcg
     2401 ccacgattct gggcgatgga ggcgtttcgt tgattctgga cgtatcggcg ttgatgcgag
     2461 ggctggcctg atcgctgcgc atgggcccgc gtgccgcagt ggcaggggcc ggagcggtaa
     2521 gggtgcaacg ggaggacact atggaagatt cattacaggg ccaggatggc ggaaacagcc
     2581 gtgagtttct tgcttttacg ctgggtgcgg aagaatatgg catcgatatc ctgcgcgtac
     2641 aggaaattcg tggttacgaa ccacccacgc gtattgccaa tgtgcaggaa ttcatcaagg
     2701 gcgtgatcaa cctgcgcgga ctgatcgtgc cagtggtgga catgcgcatc aaattcggtc
     2761 tcggcaatgc ggactatact gatttgacgg tggtgatcat cctgaacatc aatggtcgca
     2821 tcgccggcat cgttgtggac agcgtgtccg atgtgaccac gttgctgccc gaccagatca
     2881 agcctgcgcc atcggtcagc agtgcgctgg atacagatta tctgattggt ctgggcacga
     2941 tcgacgaacg catgctgatt ctggtcgata tcgaacggtt gatgaccagc gaggaaatgg
     3001 gactgactga tggcgcgctc gcccgtttct gaggcgatga tgattacaaa tcaataacta
     3061 ataatataac ggaggacaat ccatgttcgg ccacatgagt atcaagatgc gcatgaccgc
     3121 gctgctgttt ggcctgacgg gcatgctggt cattctgggc gcactcggtg tatacaccgc
     3181 gaatcacagt gttaccgtgc tgacgcagac ggtgattcgt gacaaggatt cggaagccgc
     3241 tgtggcgcgc gtcaaagtgc gcatggaagt aatccatgca cagttgctgg cggcgctgga
     3301 acacaatccg gcattgccgg tttcccggct gcatgatcat ccggtgaccg atcacctgga
     3361 cgccattgcc aaaagccgtg ccatgatgca gacgtttttt gacagctatg cgaacggcgt
     3421 gaccgatccg ggcgaaaagc gtctgctgga tctctggttg gccgacaccg acaattttgc
     3481 ccagaatgcc gtaaccgagg cggtgaccga tatccaggcc ggacaatggg atcaggccga
     3541 ggccattgcc gtgaaccgga ttcgcccgct gtacaagaat ggcgacaagg attcgcaagc
     3601 catcaaggat tatctggcgc atcgtgaagc agtcaaccag gagcacgtca acgatatgct
     3661 gacctggatg aacgaagtga tggtgggtgt catgttgttt gccgttgccc tggcagctgg
     3721 cgccggttat ttcattacgc gcggtatcgt cgtgccgctc aatcaggctg tgcaggtcgc
     3781 ccgcagggtg gcggcgggcg acctgaccgc gtctgcggtg gtcgacatgc atgacgagat
     3841 cggcgaactg caggttgcct tgcgtgacat gaacggcaat ctgacccgga tcgttggcga
     3901 ggttcgcgcc ggaaccgaga gcatttcatc cgcgtccggc cagattgctg caggcaacct
     3961 cgatctgtcc aaccgtaccg aggcccaggc cggttcgctt gaggaaacgg catcggcgat
     4021 ggagcagctg acctcgacgg ttcagcagaa tgcggaaaac gccagtcagg ccaaccagct
     4081 tgccgttgcc gcgtccgaag tggcggtgcg cggcggtgaa gtcgtctcgg aagtggtaca
     4141 gaccatgggc gcgattcatg aatcggcgcg caagattgcc gacatcattg gcgtgatcga
     4201 cggcatcgcc ttccagacca acattcttgc gctcaacgcc gcagtggaag ctgcgcgtgc
     4261 cggcgaacag gggcgcggct tcgccgtggt cgcagcggaa gtgcgcagtc tggcgcagcg
     4321 cagcgccaat gctgcaaagg aaatcaagca actgatcgac gattcggtgg acaaggtgga
     4381 aaccggtaac cgccaggccg gacaggctgg cgccacgatg agcgaggtgg tgagcagcat
     4441 ccagcgtgtc acccgcatca tggcagacat cacttcggcc agccaggaac agagcagcgg
     4501 cattgagcag gtgaaccgcg ccattggcca gatggacgaa acgacacagc agaatgccgc
     4561 gctggtcgag caggcggcgg cagcggcgaa atcattgcag gatcaggcca gccgcctcga
     4621 agggttggtg cacaagttca ggctgagcgg agccgtcgag caccggccgc agttgcgcaa
     4681 cgatcgccac ccggcagtgg cgcgtcctgt ccgtccaccg gtcagcagac cggccgtcgc
     4741 cacgtctgct gccacaacgt cagggcgcaa attgccttct gccacgcggc ctgcgcctgc
     4801 cgtccggcag gcagatgacg aggattggga agagttctga gatgcatgtg aaaaccaggc
     4861 tgcaagatgg ggggaaggat gatgaataac atcggtgtgg gcgttcgcct cgggttggca
     4921 tttggactgg cctacctgtt catggtggta ttgacgctgg tggcattttc cggagtggaa
     4981 catgcgctgg ccatcgtgat cggactgctg gtgctcggcg gaatattggg tgtgatcatc
     5041 ggtatcgtct ttgtgcgcag cattaccgat ccgctgtgca aggcagtgga ggtcagcaat
     5101 cagatcgcca agggcgacct gaccgcgcgc atcgatttcg tgtccaaaga cagcctcggt
     5161 cgcctgttcg atgcgctgcg cgaaatgaac ggcagcctca acaccctggt gggcgaagtg
     5221 catctcgcca gtgaaggcat tgccggggcg acaggcgaaa tcgctgcggg caatctcgat
     5281 ctgtccaacc gcaccgaggc gcaggcgacg gcgttgcagc atacggtggc gaccatgaac
     5341 gagatcacga ccacggtcaa gcagaacgcg gaaaactccc agcttgccaa cagtcttgcc
     5401 gtcgaagcat ccgaggtggc tgtgcgcggt ggggctgtgg tgtcggaagt ggtgcagacc
     5461 atgggtgcga tcaatgaatc ggcgcgcaag attgccgaca tcattggcgt gatcgacggc
     5521 atcgccttcc agaccaacat tcttgcgctc aacgcggcag tggaggcggc gcgtgccggc
     5581 gaacaggggc gcggttttgc cgtggtcgct gcggaagtac gcagtctggc gcagcgcagt
     5641 gcgggtgcgg ccaacgaaat caagcaactg atcagtgatt cggtaagcaa ggtggaaatg
     5701 ggcaatcagc aggtggctca ggcgggttcc accatgcagg acgtggtggc aagcatcaac
     5761 cgcgtgactg aagtggtcag ccagatcagt catgcaagcc atgagcagag cagcggcatc
     5821 gaacagatca atcgtgccat cagccagatg gacgaaacga cgcagcagaa cgctgcgctg
     5881 gtggagcagt ctgctgctgc cgccaaatca ctgcaggacc agacccggca cctcgaacag
     5941 caagtgcagc gttttaacct gtcggccggt tttggtgccc gcaattcacc gcgcccggca
     6001 ttggcggttg ggcgttcacc aggggggccg gtgcgcagat ctgtagcccg tttgccgggc
     6061 aaccgctaac gcggcacagg gttgtgttac gcaacatcgg aacgatataa gaacgaatag
     6121 aggtcgcata tgctgaataa tctgagtatc aggaccagac tgatttttct gataggttat
     6181 ctttgcctgt caatgctggt tggtggggcg atgggcatat acatgctcac ttcgtccaat
     6241 gatgtgctga aaaccattta tcaggacagt tacagcaaga tcaatgaact gaattcgatc
     6301 agccggcaga tgaaccagaa ccagatgctg ctggcgcgca tcgtgatggg caaactgtcg
     6361 aaaaccccgg acaccgatgc tgtaacggac cgcttcgtgc aggcattttc cgacaactgc
     6421 tccggcgtgt cgcaggggtt ggccagactc aagcgttatg agtggcagcc ggaagagcag
     6481 gcgctgctcg agggtatgga agacgcgctg acccactaca aggcaagcgg cgtaagcgag
     6541 gtgctggcgg cattgcacag tggccagtat ccggatgcgg ccagcgtgtt gcagggatcg
     6601 gcgcatgaat tgcagattac gctcaaccag cagacggacc tgctggtgca gcttgaattc
     6661 cagaatgcca cgctggatta ccagcgggcc cagcacaatt acaatctgct gcgtgcgttg
     6721 gccatcgtgc tgacactggt gggcatggtg gtggcaaccg ggtttggcgt ctggatcatg
     6781 cgatcgatca cccttccgct ggctgaggcc gtgcgcactg ccaagcgtgt cgcgtccggc
     6841 gatctgagcg aacaggtacg cgtgagcggc agtcacaatg aaatcgatgt gctgttgcaa
     6901 gccatgcgcg agatgaccca cagcctgcac aacatgattg gtcaagtgca tcaggtgacg
     6961 gaaatcgtac tgcatgaatc ggaaaacatc gccactggcg tcgatgacct ggcgcatcgt
     7021 accgaacagc aggccaactc gctgtccaac acggccgagt ctacggaaca catgacggga
     7081 acagtcaagc agaacaccga gagtgcccat catgcactca aggtggcagg taccacgcgg
     7141 gaaatcgccg atgccggcgg caagaccatg caggaagtgg tgctgaagat ggccgagatc
     7201 agtaccagtt cgcgcaagat tgtcgatatc atcagtgtga tcgaaggtat tgcattccag
     7261 accaatatcc ttgcgctgaa cgctgcggtc gaagccgcac gtgcgggcga gcagggacgg
     7321 ggcttcgcgg tggtggccgg tgaggtgcgc aacctcgcac agcgcagttc ggctgcggcg
     7381 aaagagatca agagcctcat cgatcattcc gtcaacagcg tcaaggccgg atcgaacctg
     7441 gtacagcagg ccggtgacaa catggaagaa atcctgaccg tggtcggcct gttctccgac
     7501 ctgctcgaaa cgatctatca ggcgtcgagc cagcaaagta ccggtatcgc cacgattaac
     7561 cagagcattg ccgagatgga caacatgacg caacagaacg ctgcactggt cgagcaggca
     7621 gccgcggcgg cgcaaaacat gcgcaatcag gccgcgatac ttgaaaaagc ggtcagcatg
     7681 ttcaagctgg ggaatctggc aggcagcgcg tcgtatgcat ccgatcagga agacgcatca
     7741 ggcagtctgc cggccaacga gcctgagccc acgtccacat tcaagcaact gagcgacatc
     7801 gaaagtgtcg gggaagagtg gcatgaattc tgactacatg tcggcagtcg cgcaaacgca
     7861 ggcatggaac acatgcgcga gccgatttgc gcatccctga gggatgcgat acatggctga
     7921 agtgtcttcc gccagggcca tcggacagga atttctcttt acagcgacgg attttgcccg
     7981 cctgcgcagc atgatttatc agcgggcggg catctcgttg agcgatcaga agcaggagat
     8041 ggcctacggg cgcctcgcgc ggcggctgcg tgtgctgggt tatgccgatt tcagtgcgta
     8101 tctggacgac ctggaacgtc atccggacag cgaagagtgg gtgaatttca ccaatgccct
     8161 gacgaccaac ctgacgtctt tttttcgcga agcgcaccat ttttccatgc tggaggatta
     8221 tctgcgcagt atccggcctc ctgtcagaat ctggtgtgcg gcaagttcaa cgggcgagga
     8281 ggcgtactcg atcgccatga ctgcttgcga agtgtgggac agcctgtcac caccggtaga
     8341 catcgtggca agcgatatcg ataccaatgt gctggccacg gccgggcgtg gcgtgtatgc
     8401 agcggaacgc gtaaaccggc tgtctccgca acgcatcagg cgcttttttc tcaagggatc
     8461 gggcaagaat gacgggttgg tgcgcgtgcg gccggaattg cgcagcatga tcagtttcga
     8521 acagatcaac ctgctcgatc atcgctggcc ggatcatggt ccatacgacg cgatattctg
     8581 ccgcaatgtc atgatctatt ttgacaaacc gacccaggcg ggggtacttg cccgttttgc
     8641 gtcggtgatg aagccgggtg gctggctgtt cgccggccat tcggaaaatt tcatcaacat
     8701 ttcgagtgat tttaccctgt tgggaaaaac ggtatatgtg ttacaggaat ccgtcaaccg
     8761 gatgcggatg gacacgcgag ggatgcgctg atgggatggg gcagggcgga ggagcatttt
     8821 gcaaccaacg tatactatga tcccgcgttc aaatgccagg ccgccaagct gctgccggga
     8881 gaatattact atactgacaa ggacatgctg atcgtcacgg tgctgggctc gtgcgtctcg
     8941 gcctgcatac gtgatccaca gctcggcctg ggcgggatga accacttcat gctgcccgat
     9001 gctggcagcg acgccggctc gaaggatggc gtttcgatgc gttatggcaa ttacgccatg
     9061 gatgtgctca tcgaagacct gc
//
LOCUS       DAIANL010000054         2647 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_55058, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000054 DAIANL010000000
VERSION     DAIANL010000054.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55058"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          467 a          739 c          874 g          567 t
ORIGIN      
        1 gggtgccgac gattacatca ccaagccatt ttcgccacgg gagttgtcag cgcgcatcaa
       61 ggcggtactg cgtcgccgcg cgccgcagat gacggaagac cgcgtcgagg tgcgcggctt
      121 gagcctcgac cctgtcagtc atcgcgtcat cggcaacaat attccgcttg aacttggccc
      181 gaccgaattt cgcctgctgc atttcatgat gacgcatccg gaacgtgtat actcgcgggc
      241 gcagttgctc gaccacgtct ggggcgatca cgtgttcgtc gaggagcgca ctgtggatgt
      301 gcatatccgg cggctcaggc ttgcactcga agcctcgggc catgagcacc tgatcgagac
      361 cgtgcgcggc gccgggtacc ggttgtcagg gcaggtgtag attgccgcgg gccgcggaat
      421 gttgatgtgc gggcgaaggc aatgcgtacg ggatgcggcg aacgggaatt gtcggtggga
      481 ctggcaggtt gcgatgaccg cgctgtgtcg cagatggcat gccgttctgg ttttgcgtcg
      541 gtctgactgg atcgcggcca gctggtgaat gccgggcgca tgccagcctc cctggaggat
      601 attttgtcgg atttctggtg gaacccggtg tttttgctgg ctgggctgct gttggtcatg
      661 ttggcggcag gcgtgatgtt cggtccggtg gtggcgttgc tgctgggcct gctggtgatg
      721 ctgctgtata tcatccgtca cctgcgctat atggccgaca tgatccgctg gctggccgat
      781 ccggaaatgt cgtccgcact tcccgccgcc agcggcgaat gggatgagat cttctacagc
      841 cttgatcgca tggtgcggcg caacaaacgc agtacggcgc gcctgttcgc cgtgctcgac
      901 cggttcgagc atgccgcgca tgcgataccc gatggcattg tcatgctcaa tgagctcgac
      961 cagatcgact ggttcaatcc ggtggctggc cgtcatttcg gttttgatga aaagatcgac
     1021 cgcggtcagt tcatcggcta cctgattcgt cagtccgttt ttcacgatta cctggcgcat
     1081 ggcgactacc gcgaagcgct ggtcatgaaa tcgccagcag accgcgacgt gacgctttcg
     1141 gtacagatgg tggctttcgg cgaacggcag aagatgctga tttcgcgcga tgtgacccag
     1201 ttcgaaatgg tggaagccat gcgccgcgat ttcgttgcca atgtgtccca cgaactgcgc
     1261 acgccgctga ccgtggtggg agggtttctg gaaaccttca tcgatactcc ggacattccg
     1321 catgcggatt ttctgcatta ctgtgacctg atgctgcagc agaccgatcg catgcgccgt
     1381 ctggtggaag acctgttgac gctgtcgcgg ctggaaagtc cgcagaaccg gctggtggaa
     1441 acgcgtgtgg acatgcctgc gctcatccag tcgctgtacc aggatgcgat cagcctcagt
     1501 gcgggtcgac atccgattga tctgttgcgt gacagtgagg atgatgtgct gggcaatatt
     1561 gacgaactgt ccagtggtct gggtaacctg gtcagtaatg ccgtacgtta cacgcctgct
     1621 ggaaaggcca tacgcctgcg ctgggggtgg cgtggcgacg agctgtgttt tgatgtatgc
     1681 gatgaaggcg agggtatcga accacagcat attgctcgcc tgaccgagcg tttctatcgc
     1741 gtcgaccgtg gccgttcgcg cgagacgggt ggcaccgggc tcggactggc cattgccaaa
     1801 catgtgctga cgcgtcacca tgctcgactg agcatcgaaa gcaaggtcgg tgaaggtagc
     1861 cgttttagcg cctgttttcc gcccgagcgg gtggttgggc agaccgggtc aggtcccggg
     1921 ctgccgcgtc aactccaggc caagctcccg gcatagcgcg tccgttgaca acgttttcac
     1981 cgcgtccggg gtggtgttac gccggggtac gctccagatc ggatcaggaa agttgcggtc
     2041 gtcacggaaa cgcggaatca cgtgccagtg caggtgcggt accagattgc ccaggctggc
     2101 gaggttgacc ttgtccggct gtagcagggt gcgtagcgcg cgttcggtcg cgaacaccac
     2161 ccgcatcagg tgcgcttgct gtgacgcatt cagatcggtc atttcgcgca catgcgcctg
     2221 ccagatgacc cgacataaca ccgggtagcc ggtttcgtgc gcaaggatga cgcggcagtg
     2281 cgtgtcctgc cacagcgggg tttcgctgac gggctggcac aatgggcaat cggtcaggtg
     2341 catgcgacaa aattcagcac gggtcgcaac ggcgccggtc atacagggcc tgcgccagcg
     2401 taccggaatc ggtatgttcg agttcgccgc ctacggggac gcctcgggcg atgcgactga
     2461 cctggatgcc gcgtggacgc agcagttccg tgatggcatg tgccgtggct tcaccctctg
     2521 cggtaaaatt cgtcgccagg atcacctcgc gcaccgaacc gttggctgca cgttgcagca
     2581 accggtcgag atggatttca cgcgggccga taccgtccag cggtgagagg cggcccatca
     2641 gcacgaa
//
LOCUS       DAIANL010000055        10278 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1078457, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000055 DAIANL010000000
VERSION     DAIANL010000055.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 10278)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10278)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10278
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078457"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2102 a         3426 c         2863 g         1887 t
ORIGIN      
        1 atcatcgacc cgcgcacggg ccagcgcatt gcattgccac cccgcatcgc caaccatgtc
       61 gacattcccc cggaccaact gaaaacgatc gttgacgcca tgatcgacgt tacccgtccc
      121 ggtggcactg ccgccagagc aagcgcgggc gcaaactacc tgatcgcggc caagaccggt
      181 accgcccagg tcgtcggcat ccgccagaat gaacggtaca atgcgtcacg catcgacgca
      241 gcccaccgcg accatgcggt attcatcgcc ttcgcacccg ccgatgcgcc caagatcgca
      301 gtggccgtgc tggtggaaaa cggaggccat ggtggcacat cggccgcacc gattgcacgt
      361 gcagtaatgg actactggct actgggcaaa ctgcccacac cgcctgccag cctgaccgga
      421 aatcctcagg accccgccca tgccgattaa cgatatacgg cacacagcag gaagaaaacg
      481 tgctgcgacg gcatttgcgg ccgaaccgcg tcacggtggg ccaggactgg ccgagcgcct
      541 cggcggcata ctggcaacga tcgcacgcca cctcgattat ccgttgctgg ccatgctgat
      601 cacactgatg ctgctcagcc tgttcgtgca atacagcgcc gcagacgaaa acatgcccca
      661 tctgctcatg cacgcggcca acatgctggt cgcattgctg ttgctggtgg ccgtggccaa
      721 cgtgcccccg ccttatctca agagtctggc ggtaccggcc tatgccgtgg gcgtgatcct
      781 gctcgtcggc gtggccctgt tcggcgatgt ggtcaacgga tcacggcgct ggttacatct
      841 gggtccggtg cgtctccagc cttctgaaat cctcaagctc gccgctccct tgctgctggc
      901 gtggtatttc cagatccacg aagcctcgct gcgactgaag cactacgcca tcgccagcat
      961 cctgctcgtg gtaccggtac tgctggtcgc ccggcaaccg gatctcggca cggccatgct
     1021 gatcgccgcc tcaggctttt acgtgctgtt tctcgccggt ctgccctggc gcatcctgct
     1081 cggcgccatc gtgctcgccg caggcagtgc tccgctggta tggcacttcc tgcacgatta
     1141 ccagaaaaag cgcattctga ccctgctcga ccccatgcaa gacccgctcg gcgctggcta
     1201 ccacatcatc cagtccagca tcgccatcgg ctcgggtggc gtgttcggca agggctggct
     1261 gcacggtacc caggcgcacc tcgacttcct gccggaacac actaccgatt tcgtatttgc
     1321 cgttttcggc gaagaattcg gcctgatggg ctcagtgctg ctatcattgc tttatcttgc
     1381 cattgtgctg cgtggacttg ccatcgcggc cagggcgcca gacctgttcg cacgtctgtt
     1441 gtccggcagc atcacactgg catttttcac ctacgcactg gtcaacatgg gcatggtctc
     1501 cggcattttg ccggttgttg gcgtaccatt gccgatgatg agctacggag gcagcgccat
     1561 ggtgattgtc atgctgggat tcggcatggt tatgagcgtc aagacccatc gcaaactgaa
     1621 caagtcatga aacacactgt cgcccaccac cggatcaccc gccccatgag cctgcgcaac
     1681 aatcctgtcg cgcccggcgc cgcgctgctc ggtctgctgg cgctggcagg atgcgccagc
     1741 aatccgccgc acgcccccat cgtcgaaccg cagaccgtgc caccgtcagc ctcccggcag
     1801 acaacaccgc cgcagacccc atcccggacc acacgccggg ggggtggcta ctaccttgac
     1861 gacggccccg gcgatcatcc gccacccaat attgacgaca ttcctgatgc cgtgccgaaa
     1921 atcgaaccct tgcgcgccgc agccaaccgg ccctatgtcg ccctggggaa aacctatacc
     1981 cctctgaccg aaccggaacc attccatgaa accggcctgg cctcctggta tgggcgccgc
     2041 ttcgacggaa aatcgacatc ttccggtgaa acctacaaca tgtacgccat gacggcagcc
     2101 caccccaccc tgccgattcc cagttatgcg cgcgtcacct cgctggccaa cggcaggagt
     2161 gtgatcgtgc gcatcaacga tcgtggtccg tttcacagcg accgcatcat cgacctgtca
     2221 tacaccgcgg cccacaagct cgggctggta cagggcggca gcggcatggt ggaagtcacc
     2281 gcgatcaccc cgaccgaaat cagtgcaggg cgagatgtcg gcgaccatac ccgcggcatc
     2341 tttctgcaac tgggcagctt cgacaatcac gacaatgccg agcgcctgct tgccgaagcc
     2401 accacccagc tcgatcgcgg cagcgccgcg ctggtgattc tcaaccaggc gcaccattac
     2461 cgggtagcac ttgggcccta ccaggacgag gacagcgcac agcaggctgc cgaagaaatt
     2521 cagaccagaa tgaaccttaa gccggttcgg ctggtcattc gatgacaacg tttccgattc
     2581 caaattcctg cgtaccaatc accatgaaac gattcctgtc ctgttttttg ccagttctgt
     2641 gtgttctgct gcttgcgtcc agcgcatccg cgtttgaact tcctgccaca ccaccgcccg
     2701 aactcgatgc cacagcctgg cttctgatcg atctgtccag cgggcagacg ctcgccgaac
     2761 acgatgccga ccgccgcgtg gaaccggcct cactgaccaa gctgatgacg gcctacctgg
     2821 tgtttggcgc gctcaggcag ggggtgatcc atcccaacga caccgtcctg ccttcgacgc
     2881 atgcgtggaa aaccgaaggt tcgcgcatgt tcatcgaacc gggaaaaccc gtcaccatcg
     2941 acaacctgct gcatggcatg atcgtacagt caggcaatga tgccactgtg gcactggccg
     3001 aacgcctggg gggcagtgaa gccgggttcg tcagcatgat gaaccaggcg gcacagcaac
     3061 tgggcatgac ccacacccac tttgtcacgg ccaccggact gcccgatccg caacactaca
     3121 ccaccgcacg cgatctttcg cgcgtggcga gtgcaatcga gcgcgaattt ccgcaatacc
     3181 tgccactgtt cgcgctgcgc gacttccgct acaaccacat cgaccagtcc aatcgcaacc
     3241 ggttgctgtg gaccgatccc agtgtggatg gcatgaaaac cggtcacacc gaatcggcag
     3301 gatattgcct catcgccagc gcccaccgcg gtggacggcg gctgctgtcc atcgtgctcg
     3361 gcacgccgag cgatgcatta cgcgcatctg aaagccagcg cctgctcaat tacggatttc
     3421 aggcatttga cggcatccgt ctggtgcgcg ccaacacggc gctggccagt ctgcaggtgt
     3481 ggaagggaaa gcgtgcgcac gtacgggcgg gatcgctggg caacatcgac ctgagcgtac
     3541 cgcatggccg cgccaaagac gtcaagatca gcctgaccac ccgccagccg ttaattgcac
     3601 cgctgtacgt cggccagacc attggcacga tcacggtgag ccttgacaac cagacactgg
     3661 ccagctaccc gctgcaatcg ctcgacaatg ttcccgtggc gagcgtgttc ggccgtgcgt
     3721 gggattcgct gatgctcaag ttcaagtgag ccacacatgc cggatgacag tctgatcgaa
     3781 tacccatgcc acttcccgat caaggtgatg ggactgcacg tcgagcagtt tcacgccgcc
     3841 atcgtggaag ctgtgctgcg acatgcgccg gatttcgaca tcacaaccgt ggaatgccgg
     3901 cccagccgtg aaggcagata cctgagcctg acctgcaccg tgaacgccac ctcgcgcgaa
     3961 cagctcgaca atctctaccg cgagctgagc gcacatccgc tggtcaaact ggtactctga
     4021 atggaacacg aagcaccatc gatcatccat atgcccggac tggtcgaata caccgccacc
     4081 tggcaggcga tgcaggactt cacggcgcaa cgatccgctg acacgcccga cgaactctgg
     4141 ctgctacagc atcccccgat ctacacgctg ggccaggcag gcctgaccga acacatgcat
     4201 cagcccaatg ccattccgct gatccacacc gatcgcgggg gccagatcac atatcacggc
     4261 cccggacagt gggtggtcta cctgctcatc gatctgcgtc gtcgtggtta cggcgtgcgc
     4321 gaactggtct ggcgcatgga gcagaccatc atcgatctgc tcggcgaata tggcatcacg
     4381 gctcagcgac gcgaacatgc gccaggcgtc tatgccgacg gcgccaaaat tgcttcgctc
     4441 ggcctgcgcg tgcgtcacgg gtgcagctac catggactcg cgctcaacac cgacatggac
     4501 ctgacaccgt tcaatgcaat ccatccctgc ggttaccgcc gcctgcgcgt gactcaactg
     4561 cgagatctgg tcgacgatgt tacaatggag catgtagccc aacgcctgtt gggccacctc
     4621 acccggcata tcgtcgccca acccgaaaga caggactcct gtggctgaca acaccctgca
     4681 caagggcgcg gccaagaccg cgcgcattcc catcccgatc gtacccaaac cacggctgcg
     4741 cctgccggcc tggatccgcg ccaaggcatc caattcagcg cgcgtcggcg cactgaaaca
     4801 ggtcttgcgc gaccacagcc tgcacacggt atgcgaagaa gcttcgtgcc ccaacctggg
     4861 cgaatgcttc agtcacggca cggccacctt catgatcctg ggcgacctgt gcacgcgccg
     4921 ctgcccgttc tgcgatgtcg gtcacggtac gccactgcca ccggacgcca gcgaaccgga
     4981 caacctcgcc accaccattg ccgcaatgca gctgcgctac gtggtcatca ccagcgttga
     5041 ccgcgacgac ctgcgcgatg gcggtgcaca gcatttcgct gactgtatcc gcgccgtgcg
     5101 tgaacgttcg ccggaaacac agatcgaaat cctggtgccc gattttcgcg gacgcatgga
     5161 caccgctctc gatattctcg acagtgccct gccggaagta ttcaaccaca atctggaaac
     5221 cgtcccccgc ctgtaccgcg cggcgcgacc cggcgcagac ttcggccact cactgcgcct
     5281 gctggccgaa ttcaagcgcc ggcatccgca ggtgtcgacc aagtcgggca tcatggtcgg
     5341 tctgggcgaa accgatgacg aaatcctcga cgtgctgcgc gaaatgcgca atcaccaggt
     5401 cgatatgctg accgtcggcc agtacctgca gccctccagc caccatctgc cgctcgaacg
     5461 tttcgtcacg ccggaacagt tcgaccgtct ggctgaaatt gcacgtgaca tgggctttag
     5521 ccacgctgca tgcggaccga tggtgcgcag cagctatcac gccgacctgc aggcccgcga
     5581 agcgggtgta tagcagcaga tgtcattccg gcaccgtttg ccactcaacg ctcaacagat
     5641 acgatttttg ccgttaccag gccgttgaaa aaggtagcga ggacgcccgg atgcaaggcg
     5701 cgagaaacgc aacgacgaga catatcaaat cgataggcgt gggagtgagt atcgcagcaa
     5761 cgccgcagac gggtgccgca gtactttttc aacggcctgc cagggagcgg tgattgattc
     5821 cacacggtcg cgacgttggt ctggcaggat ggattgcaag gcacgattcg aaaccagtgg
     5881 ctgacacccc tgaccagaag cgcaacgcgg caagctgccc gccagaccct ttgggttggg
     5941 gtgaaatcgg gaagatcgct gcgcgcaggt cacaggtcgt gacccggact ccggtgccag
     6001 tccgcgcagg cccatgtaga cgcagcacag cggttccccg gcagcgctgc ggggctggag
     6061 atcactgccc tgccgccagc cggccggaca ggcttgccgt atgcgatgcc tggtagatcc
     6121 agtgatgttt ccacgccgac accaccgtga gcggatattc cggccgaccc agcgacccgt
     6181 tgtaccgccc cagcgcccgg tacaaatctc cattctcctg atcgagatac agtcgcagga
     6241 tattgcagcc atatcgcaga ttggtgcgca ggtggaacag atcctgcccc ggcgtgccga
     6301 ccagattgac ccagaacggc atgacctgca tgtaaccacg tgcatcagcg ctggaaatgg
     6361 catatttgtg gaaaccggat tcgacttcga tcacacccag caccagttcg ggatcgagtc
     6421 cggcgcgcac cgattcgtaa tactccgtgg cgagaaaatc gcggcgctgg cgcgcatcgg
     6481 gcatgcgctg ggcaagccgg cgcgacattt catcgatcca gcggcgactg tcggcagttt
     6541 gcagaacagg cgcatcaagc accggattgt cggacactgc ggcactcagg ctggcgcgca
     6601 cgctgtcgga cagcggttcg tagatctggt gaccagcgca ggccggcgcg gctggcagca
     6661 atagcactgc cagcccgagc agaaggagct tcaacccacc agtctttgca ccagccatgt
     6721 cacgccccct tcgacaggca cccgctgcgc ttcgctgtcg ccacgtgcct ggaattcgaa
     6781 ctcgcctgtg gccagtgttt tgtcaccgat caccagccgc gccggcacgc caatgagctc
     6841 catgtcggca aacatcacgc cggcccgctc atcgcggtca tcgagcagca catcgacgcc
     6901 ctgcgccacg agatcggcat acaggcggtc agccgcttcg cgcacacgct cgttcttgcg
     6961 ataaccgatc ggtacgattc cgagtgtaaa aggcgcaatg gcacttggca aggtcatgcc
     7021 gcgatcgtcg aaattctgct cgatggccgc tgccacgatg cgcgatacac cgatgccgta
     7081 acaacccatt tccatcggct gtgtctgccc ctgctcgtca agaaatgttg cctgcatagc
     7141 caccgaatac ttggtgcgca gctggaagat gtgccccacc tcgatgccac ggcacagttc
     7201 cagccggccg cggccatccg gactcggatc gcccgccacg acgttgcgca gatcggcgac
     7261 ttccggttca ggcaggtcgc ggccaaaatt gacaccgctc cagtgaaacc cgtcttcatt
     7321 cgcaccgcag acgaaatcgc tcatcaccgc cacgctgcgg tcggccacca cacgcacacg
     7381 cagcccgacc ggccccaccg atccgggagc acagccggaa gccgcacgca ccgcagcctc
     7441 gctggcaaaa cgcacttcct ggccaagcag cttgcccgcc ttgacctcat tgagctggtg
     7501 atcgccgcgc aacagcaatg caacgatacc gtcgccacat tccaccagca gcgtcttgac
     7561 cagctgcgag ggctcgtgac ccatggccga tgccacgtct tcgatgctac gtttgccggg
     7621 cgtagcgact ttgtgcatcg cctgcccagg cacagcacgc ttgccatcgg gcgcaacggc
     7681 ttcggccagt tcgacattgg cagcgtagtc agaatcagga cagaaggcaa tcgcatcctc
     7741 acccgaatca gccagcacat gaaattcatg cgaacccgaa ccgccgatgg caccggtatc
     7801 cgccgccacc gcgcggaaac gcaacccgag ccgggtaaac acccggctgt acgtctcgta
     7861 catgacgcgg taggtcgcct ccagactggc gaggtcggca tggaacgagt aaccgtcttt
     7921 catgacgaac tcgcgcgcgc gcatcacgcc aaagcgtggg cggatctcgt cgcggaactt
     7981 ggtctggatc tgatagaaca tcagcggaag ctgacggtaa ctcctgacct cacgacgcgc
     8041 cagatcagtc acgacttcct cgtgcgtggg gccaaaacag aattcgcggt cgtgccggtc
     8101 gaggatcttg agcatttgcg gaccgaactg ctcccagcgg ccggtttcct gccacagttc
     8161 ggctggctgc accgccggca tcagcacttc caggccgcca gcacggtcca tttcctcacg
     8221 cacgatcgcc tcgaccttgc gcagcacgcg caacccgagc ggcatccagg tatacaagcc
     8281 cgagcccagt cgccggatat aaccggcgcg cagcatcaac cggtggctca ccagttccgc
     8341 ttccgccggc gcttccttga gggtggaaag aaaaaatcct gatgcacgca tgctgtgttt
     8401 tccgtaccgg tggcaaaagg gttattctat cgcataaccg cgcatcaaag tcgtgcgcgg
     8461 ttcagccgcc tgtcaatccc gggcaaggag tccccaatca tgcgtttttt ttcgcgacgc
     8521 atccacaaag ccgcgtccac cgacctcgat accgtcgaaa tcagcgagca gcgcggggta
     8581 cgttatctgc atctgggcaa tgcaaccatc cagtcagcca tgcgtatcca gtcgcccaac
     8641 acgctcgaac tcagctacac ccaggccatg accgccttcc tgctgttcgg tattcccgcc
     8701 agcagcgcgt tgatggttgg gctgggcggc gggtcgctgg ccaaattttt ctaccaccag
     8761 tatccgcacc tcaagctgtg cgcggtggac atccacccgg aagtgatccg cgccgcctac
     8821 cagttcttcg aactgccgcg tgacgagtca cggctgcagg tcatccagca ggatgccgcg
     8881 cagttcatgc ctgatcagcg cggctgggac gccatcctgc tcgacggtta cgacgcccac
     8941 ttccaggtgc cgcaactggc cagcgaaacg ttctaccggg actgcgccca ggccatgggc
     9001 gcccggggcg tgctgtcggt caacctgtgg ggcagcgatc cacgttttac ggtataccgc
     9061 gaacgcatgg aacgcgtctt cgatggacgc gtgctgacct tgccggcaga aaaacgcggc
     9121 aacgtgatcg cctttgcctt caacgaaacg cccgacgccg tttcctggaa ccggctgcac
     9181 cagcacgcca tggaactgga gatgctgcac cgcatgccat tcacccgttt cctgcaacac
     9241 ctgcgaccgc aattgcgctg gatgggtatc gactgacccg ctcagaccgg agtgaccaaa
     9301 ccgcgcatga catgcggcgt tccggactgg ccggttgttt ctggtcggtc agacgacttc
     9361 cctcgcaagc ggccagtcct tcgcctgacg tgttgataga tgcccgtaat tttgcggcag
     9421 gacgcaagat gatcttcatc gcctcacgcg gttgcagatt tccagtcgtc cccgaacatg
     9481 atccgtttca tgaactcgtc aaagggcggt agagcaaaca cagtatcgtg atggctcgtt
     9541 acctcatgat gccgttatct tgaatttctt ctccgcaagg ccgcaaacag acaaaaaaac
     9601 acccccgcaa gcgggggtgc atgtgcatcg gaatgaccgt accgactcag gacttgcgct
     9661 tgcgctcgat catcttctcg atcatcggtc cgaggatcag ttccatggca tagcccatct
     9721 tgccgccagg aaccaccagc gtgttggaac gcgacatgaa cgagttggga atcatgttga
     9781 tcaggtaagg aaaatcgacg ccacggtggt cgttgaaacg aatgatgacg aagctttcat
     9841 ccggcgtggg aatgtcccgg ctgatgaacg gattggaagt gtcaactgtc ggcacacgct
     9901 ggaaattgac atgcgtgcgc gagaactgcg gcgtgatgta attcacataa tccggcatgc
     9961 gacgcaggat ggtgtcgacg atcacctcgg ccgaatagcc gcgttccgcg ccatcgcgat
    10021 gaatcttctg gatccattcc aggttcacca ccggcaccac gcccacgcca aggtcgacat
    10081 ggcgcgccac gtcgatcttg tcagccgcaa ccagcccgtg cagaccttcg tagaacagca
    10141 gatcggtgcc cgatggcacg tcttcccatg gcgtaaattc cccggcggac agctggctgc
    10201 cgagacgctg gttatggaat gccgcctctt cgtcactgtg aatgtagtag cgctttttgc
    10261 caccacccgt ttcgccat
//
LOCUS       DAIANL010000056         5003 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_58008, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000056 DAIANL010000000
VERSION     DAIANL010000056.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58008"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1029 a         1693 c         1369 g          912 t
ORIGIN      
        1 caccaaccag cgtgaagaca tctggggcgg cagtctggaa aaccgtgccc gactgctgct
       61 cgaagtcact gacgccgcga tttcagtatg gggtgcaggc cgggttggcg tgcatctttc
      121 gccgcgctgc gatgcgcacg acatgggcga ctccaacccg ctcgccacgt tcagccatgt
      181 cgccgaacaa ctcggccggc gcgggattgc ctttatcttc acacgtgaat cgctgggcga
      241 gaatcgcctg agcaacgaac tgagaaaacg tttcggcggc atgctcatcg ccaatgaggc
      301 gttcgatgcg ggcagtgcca gacacgaaat cgaaaccagc tgtgcggatg cagtcgcctt
      361 cggcaaggat ttcattgcca accccgacct gccacggcgt ctgctgcacg gcctggcgct
      421 caatccgctg cgcaccgact cgatgtacgg ttacggcctc gacaacccgg aaaccggcta
      481 caccgattac ccggcgctgc aggactaagc gccatgcgcc actctgccgg ccgcgagaat
      541 cgcggccggc agttctccca gtgacagcga gcgttcgacc gcgcccagct tcaccgcttc
      601 ctttggcatg ccgtatacca cacaggtggc ttcatcctgc gcgatggtac aggcaccagc
      661 ggttcgcatt tctttcagcc cgcgtgctcc gtcatctccc atgccggtca tgatgatgcc
      721 gagcgcattc cggccagcgc accctgccac cgaacggaac agcacatcca ccgacggccg
      781 gtgccgattg accagtggtc cgtcgatcac ctcgacatgg tactgcgcac cgctgcgctt
      841 gagcagcata tgtcgaccgc ccggcgcaat cagcgcctgg ccgggcagca cacgctcgcc
      901 gttggacgct tcgcgcacgc tgatgcggca cagaccatcc agccggtgcg caaatgcgcc
      961 ggtaaatttt tccggcatgt gctgcacgat gacgatgccc ggacagttcg ccggcaatgc
     1021 cttaagcacg gcttcgagcg ccagtgtgcc gccggtcgag gtgccgatcg ccacaatccg
     1081 gtcagtggtg tgcaccatcg ccccactggt cgacggcagc accgcatccg cggacagctt
     1141 cggtgccacc ggagccggcg ccacgctggc gagcaaccgg tgcacattgg cgctggccgc
     1201 agccttgacc gtctgaatca ggtcttccga tgcatcgcgc aaaaattccc gcaacccgag
     1261 cctgggtttg gtcacgatgc tgaccgcgcc ggcagccagc gcctgcatcg aggtttccgt
     1321 tcccgctccc gtcaatgtcg aacagatcac caccggcatc ggccgttcgg ccatgagctg
     1381 gcgcaggaag gtaatgccgt ccatgcgcgg catttccaca tcaagcacca tcacatccgg
     1441 tcgcagcgca tcgagcttgc cacgcgcaaa cacagggtcg gctgcggtac cacacacctc
     1501 gatccccgga tcagcggcca gcacagctga caacatctgg cgcatgagcg ccgagtcatc
     1561 gacaatcatt acgcgaatcg gtcgcacatc cgttgctcca tctcctgctg ggcacagcca
     1621 cccgattccg actccacatg cctgacccag acctggccac tccacacatc gaaagcaatc
     1681 ttgcggtgac caacgccgcc ggtatgctgt gcgacgcatt ccagcccgtg tgcctgcagc
     1741 aactcgtgcg cccgcgcaac attgcgcgca cccacatgaa tctcgctccg cgccgcttgc
     1801 gccggaaaca tgttaccgcc accgaacagc ttgatttcgc agtcacgcgg gtcgattccc
     1861 gcagcagtca ttttgcccag cagcagccgc acggcctcgt cgccatagcg gccgtcacgc
     1921 tccgccgtgc cccgcccgac tgtactgcgc tcctcggcac gtgacggcag catgtagtgg
     1981 cacatccccc ccatcctgcg tcgtgcgttc cagaaggtca gcgagacgca cgaacccagc
     2041 accgtccaga tgcgggtgtt ggtgtcacca aaatgaacat ccccaggctg gaggaaaatc
     2101 tccgcgagtc gggattcaga cgctggcatc gggtttcctg taaatggacg gcgcaacgag
     2161 gtcgaaacgc tcgctcacgc cattgaggct ttccgaatgg ctgatgatga aatgaccacc
     2221 tggccgcagc aatggataca accgttccac cacattgcgt ttggtttcaa gatcgaaata
     2281 gatcatgaca ttgcgcagaa aaatcacgtc gaaagctccg agagccggca acgcaccaat
     2341 caggttcacc tgacggaact gcacacgcga atgcaacggt ttggccacca tgagcttgcc
     2401 atcatactcg cccacgccct tgagacagaa gcgcgaccga taggcttgcg gcaggtcgcg
     2461 cgcgcgttcc agcggataga tggcgcgtgt cgcggtttgc agcatgcgta cactgatgtc
     2521 cgaaccgagc acctcccagg cactgtcgcc gagctgatcc tgcagcagca tggccaggct
     2581 ccacacttct tcgccggacg aactggccgc gctccagaca cgaaacggag atttgccgtc
     2641 atgcgccgtc agaatgcgat cacgcaggaa atcaaaatgt cgcgcttcac gaaagaaaaa
     2701 ggtttcattg gtggtcagca gatcgactgc cgtctggaat tcttcaccgc cctgcatcac
     2761 cagacgaaaa tattcaccat agctgcccag accatgttcg cgcacacgcc tggccagccg
     2821 cccacttacc agtggcttct tgctgtcggc catgctgatg ccggccacct ggcgaatcat
     2881 ggactggaac tgggaaaatt cgctgtcact gagcgtcaat ccattcattg ctggctgccc
     2941 catcactgca gtagtcctgt ctgttcaaaa tcggtgcgcg gcatcggtcg gccatacaga
     3001 tagccttgcg ccatgtcgca cccgtgcgac ttgagaaatt cagcctgttc gacagtctcc
     3061 acgccttcgg ccacgaccac ctggccaagg cagcgagcga tcgacagcat ggccttgacc
     3121 agttccgcat gcgatgcctg ctcgcacatc tggctgatga acgaacggtc gatcttcagc
     3181 gtcgtgatcg gataacgcgc cagataattc agcgccgagt accccgtgcc gaaatcatcg
     3241 atggcgatgc caatccccat gtcgcgaaac gtcagcagcg cctgcagcac attactggtt
     3301 tcgtcgagca acaggctttc ggtgatttcc agttcgatcc attccggttc gcaacccgta
     3361 ttttccagaa tgcgccgcaa cctgtcgacc aggtcgggca actgaaactg gcgcgccgac
     3421 aggttgattg ccatgcgatg cccgccgggc gaggctgcgt tccattcccg cgcgacgcgg
     3481 caggcctgat gcagcaccca ttcgcccacc tcgaaaatca gtccggtctc ctcggccagt
     3541 ggtatgaagt aaccgggtgg aagcaggccg cgttccggat gattccagcg caacagtgcc
     3601 tcagacccca tgagtgcgcc gtcagccagc ctcagcttgg gctgaaaata cagttccagt
     3661 tcgccacgac tgacggcctt gcgcagatcg gcttcgagca gccattcgtc gcgagcggtt
     3721 tctgtcaact tgcgcgcata gaaactgaag ctgttacgcc cggagcgctt ggcatgatac
     3781 atcgcggcat cggcaaactt gagcaggtca tcggcattgt tgctgtcctg tggatataca
     3841 gtcacgccaa tactgacgga tgccaccatc tcccggtcgc cgatcacgac cgactcctga
     3901 aaggcgtgca gcaactcccc ggcactcacg gcaggatcat cgtccgcttc gagccagaac
     3961 tgcagaatgg cgaactcgtc gccacccagc cgcgccacca catcgctggc tcgcgccatc
     4021 cgcagcaatc gctgcgccac ggcgcgcaac agcgcatcgc cagccggatg gcccagactg
     4081 tcgttgaccg acttgaaccg gtccacatcg agaatcatca gcgcaacccg gtaatcgtgt
     4141 gcagcagcct gttccagcgc atgctgcaac cgctcggcaa acagcacccg gttgggcaac
     4201 ccggtgagcg aatcataata ggccatctga tggatgcgct ggcgctgaac attcagttcg
     4261 gtgatatcgc gtcccacccc aagcacgctg acgatttcac ccgatccatt gcgctccgca
     4321 gtcaagcgta ccaggctgca cgcctcttcg ccgtcatgtg ccggccaggt gaattcgatt
     4381 tcacggtcag ccccgtcggc aaacaccgag cggatcgtct gttcgaaacg ctcggcattc
     4441 gcgccgccgg gaaactcgca cggacgcatg cccagcatga cgctggtgcc acccgcggct
     4501 ttctcgccca gcgccgcatt cacatagatg cggcgacact gctgatcgta acgtgcgata
     4561 tgatccgggt tgttttccac cagcacgcgg tattcgcgct cacgcgccgc cagcgagcgc
     4621 tccatcgcct tggcgcgggt gatgtcgcgc aatatcacgg caatgcgcca tgcactgccc
     4681 tgactgccac tcaacggaat catgctggca gaaaaacagc gctcacgacc tgcttcgcgt
     4741 aaccagacct cttcctgcag cgtgtgcgcg cgctcaaggc gacggcgatc cagccccagc
     4801 aaaaaccccg aaagcgctgg cgacagcgct tcctgcaccg gacacccagc gacggcttcg
     4861 ctgcgcaggc caaaaccgcg ttctgccgca ggattgatgg ccgccacgcg catctgccag
     4921 tctgcctcga cgtcaatgaa caggatactg tcatcgaaat tggcaaacag ctgctgacac
     4981 cgcgcatccg attcaagccg cac
//
LOCUS       DAIANL010000057         5767 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1489619, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000057 DAIANL010000000
VERSION     DAIANL010000057.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489619"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1246 a         1880 c         1583 g         1058 t
ORIGIN      
        1 ctgcccttgt agggtacgcg gccttcgatg ccttcaggaa caaatttgtc tgcgccggcc
       61 tcggcatcct ggaaataacg gtcgctcgac cctttctgca ttgcacccag cgatcccatg
      121 ccacggtagg acttgtacga ccgcccctga aacagctcga tttcacctgg cgcttcttcg
      181 gtgccggcaa acagcccgcc cagcatgacg caatgcgccc cggcggcgat ggccttggcg
      241 atgtcgccgg aatagcgcac gccaccatcg gcgatcaccg gcacatgcag cgcagacagg
      301 gcagccgcca cattctggat ggcagtgatc tgcggcacgc cgacacccgc cacaatgcgc
      361 gtggtgcaga tcgagcccgg gccgataccc accttgaccg catcggcgcc gtgatcgacc
      421 agtgccagcg cagccgcagc ggtagcaatg ttgccgccaa tgacctgcac ttgcgggaaa
      481 ttgtgtttga cccaccgcac gcgatcgagc acgcctcgcg aatgcccgtg cgccgtgtcg
      541 accaccacca catccacccc ggcttccgcc agcgcggcaa cacgatcttc ggtgcccgcg
      601 cccacgctga ccgcggcacc gacgcgcagg cgtccctggc tgtctttaca ggcgttggga
      661 tgttcggaag atttctggat gtccttgagt gtcaccaggc cacgcagttc gaaatcatcg
      721 ttgaccacca gcaaccgttc gatgcgatgc tggtgcagca gtgcctgtac ttcggcacgg
      781 cttgcacctt ccttgaccgt caccagccgc tctcgcggcg tcatgacatt gctcaccggc
      841 tggtcgaggt tgttttcaaa ccgcagatcg cggttggtga cgatgccaac caccttgccg
      901 gcctcgtcga gaacgggcag cccggaaatg tggtggcgtt cacgcagcaa ctgcacctcg
      961 cgcacgctca tgtcacgcgt gacggtcacc ggacgcttga ccacgccgga ttcgaaacgc
     1021 ttgacccatg ccacctgggc agcctgctgc tcgacggtca tgttcttgtg cacgataccg
     1081 ataccaccct cctgcgcaag cgcaatggcc atcggcgcct cggtcacggt gtccatagcg
     1141 gcggaaagca acgggatatt gagagtgatc tcgcgggtca gacgggtgga cagcgacacg
     1201 tcgcgcggca aaacatcgga aagtgccgga accagcaaaa cgtcgtcaaa agtgagggct
     1261 ttttgaatca cacgcatagc aaatgttcct gaacaaagcg ctattatacg gcaaaccggc
     1321 cgttgccgcg acagcccggc acgcgcggcg ctcaggccga cagatcgccg ccgcctttct
     1381 tcatggcctt gccggcgtcg gcacgctgac ggcgcacttc cttgggatca gcgatcagcg
     1441 gtcggtagat ttccacgcga tctttctcgc gcagcagata atctggcttg actgccttgc
     1501 cgaagatccc gatccggttt ttgctcaggt cgatttccgg aaaacgggtc agaattccgg
     1561 attgttccac tgcggtttgc accgttgtac ccacgcccac ttcaaccggc aagatcacct
     1621 gaatgtctgc tttggcgtaa gccacttcca ccttgatacg ctgctcgttc atgatgcccc
     1681 atacacccga tctgcacgtt caataaacgc ttcaacgaaa ctgttggcga tgaatccgaa
     1741 aacaggcccc agtacggtat ccagtatcct gctggaaaac tcgtaatgaa gcatgaattc
     1801 gatcttgcag gcctcgtctc ccagcgcacg aaaacgccat tcgccttcga gcagacgaaa
     1861 cggtccgtcc accaggcgaa tacggatcag cgtcggatgc tcgttgctgt tctcggtgct
     1921 gaactgctga cgaatgtgca tgtaattgat gtcgatgctc gccaccaacc ggtcttccgt
     1981 gcgcatgctg acattggtgc ccccacacca gggaaggaat tgcgggtaat cctccactcg
     2041 gtcgaccagc tcaaacatct gctgcgcgct gtgacccacc agcacgcttc tgttcaccaa
     2101 cgccatgatc cggcttgcct gcaaaaactg gtagaatacc cgattcgggg aaaaaagtgt
     2161 catgaacatc gcggaaaaca ggaaagtctg gcacgattat ttcatagaag agaaatatga
     2221 agccgggctg atgcttgaag gctgggaggt taaagccatc cgggcgggtc gtgtgcagct
     2281 caaggaagcc tacgtcgtga tcaagaatgg cgaagttttc ctgatcggcg cgcacatcag
     2341 cccattggcc acagcctcga cacacattca tccggatacc acgcgcaccc gcaaactgct
     2401 gctgcatgcc gaagaaatca gccgcctgat cggcaaggtg gaacgtgccg gctacacgct
     2461 ggtgccgctc gacctgcact acaaacgttc acgcatcaag cttgaaatcg gtctggccaa
     2521 aggcaagaag cagcacgaca agcgcgaggc gtcaaaaacc cgcgaaaccg aacgcgaaat
     2581 ccagcgcgcc atgcgcagtc acaatcgtca cggctgacgc tgcctaagct gcgtcagcct
     2641 tacgcgcagc gtgcgcaacc atcaatgcgt ccagaaagcg ctgcatggcc tgctccacct
     2701 gctgccactg cccactggtc atggcagtgg catcgatgct gcgcagcacc cggccgaggc
     2761 agtggtggta ttcatcatgc agggcgaaca attcatccgg ctcggcgcag ccgagccgct
     2821 gcattgccag gtgcatctgg cacacacggg ggttttccag cgtctccagg tgcaaccggt
     2881 cgagattgac gcgcgccatc ttcttgatcg cctggtgagc gatcagcgtt ttggcataca
     2941 gtccaaaaat ccttctgcta ccctggcgcc acgcccgggc tgcccggcaa gcagttccac
     3001 caggcgctcg atcggcaccg ggcggctgat ggcatagccc tgtacgaacg ccacacccat
     3061 gccttgcatg gcatcgagga tcggcagagt ttccacccct tccaccacca ggtcgaccga
     3121 cagttcttcc gccaggtcct ggatggcacg cacgaaatgc agatcctgcg gattgtgttc
     3181 cagcgtgcgc acaaacccct gatcaagctt gatctcgtcg atcggcaatt ccttcatgcg
     3241 cagcagcgag gcgtaggcac tgccgacatc atccagcgcc acgcgaattc ccagcttgcg
     3301 gatctcgtga aaaatggcca acgcactggt gcgttcaaga aaatcgctgc cttcgagcac
     3361 ctcgaccgtt accctggacg gatcgatgcc gctggcacgg atcacccctt ccaggcatgg
     3421 cacgcagttg ccgcagaacg attcgggagc aatattgaat gacacccaca aacgggaacc
     3481 caaagcatcg agccgcgcca gatcggcaag cgactgtgtc agcaccatgc ggctgagctc
     3541 ggtcgtctgc gccgcatcga gccgggagag gaactcggac gggtacaaca ggtcaccggc
     3601 gtcatcgcgc agcctggcca gtgcttcgac gccgacgatt tttccggtgt ctacattcaa
     3661 tatcggctgg taatgcacga ccagatgatc ctcggtaaac agccgcagta tccggtcgtt
     3721 gcgtgaccgc cagtgttcct cgccgaacag cacccagttg cgctcacgca cggccttgac
     3781 cgacttggcc tcgtacagcg cctggtcggc cagacgcagc agaccatccg gactggacac
     3841 atcgataaat gggtaaatgc acaccccgat gctgcagctg atcgacacca ccacaccgtc
     3901 atcaagcgca atcggctgtt cgaggccgtc ctgcaccctg gcaagcgcag cctcgagctg
     3961 gtccagatgc tccagatcgc tgaacagcag cacaaactca tcgccaccga gccgcgccgc
     4021 gaaatcaccg gcacgcagac tgccccggaa acggttgctg atcgtcttca gcaccgtgtc
     4081 gccggcgtca tgaccgaatg cgtcgttgac cggtttgaac ccgtccagat caagcaccgc
     4141 caccgcaagc agccgctcct gtacatgcga gcgctccatc gcccgcgcca gcacatcgcc
     4201 cagcgcacgc cggttgggca ggtcagtgag tggatcgtgc aacatcaggt gttcaaccat
     4261 cagttcgcgc gccttgcgct gactgacgtc acgaatgacg ccgacgaaat gccggatctc
     4321 gccgcgctcg gtgcgcaccg gctgaatcgt cagctcgttc cagaacggca taccgtcctt
     4381 gcgataattg agtatctcgc caaaaaacgg cagttcatgc tgcagcgcgg ttcggatacg
     4441 gtcgcggact tcctggctgg agtcggcacc ttgcagaaga ttgcaattgc ggccgcgcac
     4501 ctcatcgagg ctgtagccgg taatccgcgt aaaggcgggg ttgacgtaga agatgtgctg
     4561 ccgcgcatcc gtcatcagca caccttcgga aatggcggca aacgcagtca ccagatcgga
     4621 cgtcagctga tgttgccggt gctgttctac ccgcagcgcc aacaggcgtg ccagcgattc
     4681 cacccagtca ctctgaacgc ccgcaacaga tgcctccggg cgcaatccga gcacgagctg
     4741 gccgagcacg ctgccatcac cggcgcgcac gggttcgatg cgacacaggc catagccatg
     4801 ttccgtcagc catgcgtcca gcatgttgcg ctgagagaca gcatcaggga gcgctgtctg
     4861 gcctgcacca gccagacccg gcgcatcaac agaaaagtcg ccatgcagcg ccagcacctg
     4921 gcaccgtccg tccgtggacg tcaccagcca tgccagcagg tcttcggtgg cataaccggc
     4981 cagacccgca gcctgcttca ggatgctgcg cagatcaccg cgaaccgcgt catgcatgga
     5041 cgcgtccctg cggctggttg catatcactg cacaatacag gcattggcct ggtcgttcgc
     5101 caacgcgacc gtgatggttg gggaatctca tcaaaaaaac cgctgggcga ggacgcatac
     5161 ccagatccat acccagacat gacaaatcag cacgcaacta tagccgaacc caccggattt
     5221 gtcacaaaaa cccccagcgg caccagatca tcgcagcata tcaacgtcgc agcctgaaat
     5281 acacctcggg caatttcttc ggcaactcgt tcatgtggcg tatcaccagg taatgcccgc
     5341 taccgaagat ctgtggcaga tattcgctgc ctgcctcatc gagcgtgatg cagaacgggt
     5401 gcacgccggc atcgatcgcc tctttcactg ccatgcgcgt gtcttcctgc aaatagcgct
     5461 cgtcgccacc gtcatcgtaa tcggcaggac ggccatcgga caggatcagc agcaaacgac
     5521 gggccgatgc gaccgaatcg aactgtgcca ccgcatggcg aatggccgca cccatacgcg
     5581 tagccagacg accgctcagc ccgcccagca cggcacgctc gcgcatgccc aatacaccgt
     5641 cgaatgactt gatcggataa tagctgacgt tatcgcgata cttggacgcg aatccggcca
     5701 cagcatatgg gtcgcccacg gcttccatgc tctcggcaaa cagcagcatc gcagcgcgga
     5761 tgcgatc
//
LOCUS       DAIANL010000058        35049 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2711670, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000058 DAIANL010000000
VERSION     DAIANL010000058.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 35049)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35049)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..35049
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2711670"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         6558 a         9701 c        11274 g         7516 t
ORIGIN      
        1 cgccatgggg ctggcgaacg tggaagtctc ggaaagcgcg gcgtaccgtc atgcacaatc
       61 tccgtcgtgt cggcgattgc gttgggcata gcttacgacg gaatcattac cgtccgatga
      121 atgtgcagcg cgcgaggggg gcgcggacgg acaatgagcg ccggtccggc ctgaccggcc
      181 ggggtcgggt cagaagacga agcgttgcgg ctgctgggca tgaaccagcg gcctgatgca
      241 ggtttcgaac agcgtttcag tgcgccatga ctgttctgtt gtacgtgtga tgcgctgggc
      301 ttgcatggcc ggtgcgtcac cgacgatggc gaacaaccgc ccaccgatgg ccaattgctg
      361 caggaaggta tcgggcagaa ccggggtcga accggtcaga acgatggctt gccatggcgt
      421 ctggctggcc cagccgaggg ctgcatcgcc ggtgacgagg gagacattgg tgatgtcgca
      481 ggccagcagt ctggcctgtg cctgggcggt gaagtcgggg tgaatgtcga tgctggtgac
      541 atgcccgccg ctggcagcga gcagcgcggt caggtaaccg ctgccggtgc cgacctcgag
      601 gatgcgatct tccggccgga tggcgagttc ctgcaggatg cgggcttcca ttttcggcgt
      661 catcatcact tcatcgtggc cgagcgggat ttccgtgtcg gcgaaagcca gttcacgcca
      721 tgcggttggc acaaacgcct cgcggcgcac ctgctcgagc agatcgagca caccctggtc
      781 aagcacatcc caagggcgaa tttgctgggc aatcatgttg aagcgggctt gttcgatgtt
      841 catggcgcgt gtagtgatga atgatcgctt gcgattattc cataattccg atacttttac
      901 acctgtgcta ggggtctccg ggcatccggg gcgagataca ccctttgaac ctgacacggg
      961 tcatgccgtc gtagggaagc attcgatgct ccttacgctt tgaaaatatc aaggagcatc
     1021 tccatgagtg atcccatccg ctttactgcc gctgaagcgc aggtcgatca ggctgcagtg
     1081 cagccgtacc ccaattcgaa caaagtttac gccagcggat cgcgcgcgga cattcgcgtg
     1141 ccgatgcgcg aaatccgcca gtccgatacg ccggctgcga tgggcgcgga acccaatcca
     1201 ccggtgtacg tctatgactg ttccgggccg tacaccgatc cgacgttcaa tgccgatatt
     1261 cgcacgggcc tgccaccgct gcgcaccgaa tggatcgtcg agcgcaacga caccgggccg
     1321 ctggcccagc cgggctccgc ctatggccgt gagcggctgg cagatgccgg cctggaccac
     1381 atgcgtttcc ctggcctgtc gcgcaagcca cgccgcgctg tgccggggcg cgtggttacg
     1441 cagatgcatt atgcgcgtgc cggaatcatc acgccagaaa tggagttcgt tgccatacga
     1501 gaaaacctgc agcgcgagcg ttaccttgaa tcgctgaaag cgtccggcgc aaccggagag
     1561 cggttgcatg ccctgctggc acgccagcag ggcggacagg cgtttggtgc ggcgattccg
     1621 aagagcgtga ctccggaatt cgttcgcgac gagatcgcgc gcggtcgcgc catcctgccg
     1681 gccaacatca atcacccgga agccgaaccg atgatcatcg gccgtaactt cctggtcaag
     1741 gtgaacgcca atattggcaa ttccgcactt ggatcgtcga ttcaggaaga agtggaaaaa
     1801 atgacctggg cgatccgctg gggtgccgat acggtgatgg acctgtccac cggcaagcat
     1861 attcacgaga cgcgcgaatg gatcgtacgc aactcgccgg ttccgattgg cacggtgccg
     1921 atctatcagg cactggaaaa agttgatggc aaggcggaag aactgacctg ggagatgttt
     1981 cgcgacaccc tcatcgagca ggcggagcag ggcgtggatt acttcaccat tcacgccggg
     2041 gtacgtctgg cgcacgtgcc gctgaccgcg cggcgcatga ccggcatcgt gtcgcgcggc
     2101 ggttcgatca tggccaaatg gtgccttgcg caccacaagg aaagcttcct ctataccaat
     2161 ttcgaggaaa tctgcgaaat catgcaggcc tatgacgtca gcttcagcct cggtgacggg
     2221 ctgcgtccgg ggtcgatcta cgacgccaac gacgaagccc agcttgccga actgaagacc
     2281 ctcggtgagt tgacgcagat tgcctggcag cgcgactgcc aggtgatgat cgaggggccg
     2341 ggccatgtgc caatgcagat gatcaaggag aacatggatc tgcagctgga gtggtgctac
     2401 gaagcgccgt tttatacgct gggtccgctg accaccgaca tcgcccctgg ctatgaccat
     2461 atttcgagtg cgatcggcgc cgcgcagatc ggctggtatg gcacggccat gctgtgctat
     2521 gtcacgccca aagagcatct ggggctgccc gacaaggatg atgtcaagga agggattatc
     2581 acctacaagc ttgcagcgca tgccgctgac ctggccaagg ggcatccggg tgcacagatt
     2641 cgcgacaatg cgttgtcgaa agcacggttc gagttccgct gggaagacca gttcaatctc
     2701 ggcctcgatc cggacaaggc gcgtgaattc cacgacgaga ctctgcccaa ggaatcggcc
     2761 aagctggcgc atttctgttc gatgtgcggc ccgcatttct gttcgatgaa gattacacag
     2821 gacgtgcgtg aatatgctgc agggctgggc gtcgatgaaa gtcaggcgct gagtgcgggc
     2881 atgcaggcca aggctgtcga gttcgtcgag atgggtgctg aaatctatcg caagtcctga
     2941 cctgcgcggg ctataatctg cgcaccgttt accaactgac aggaaaatgc cgtggatcta
     3001 ctgttgttcg tcaaggcgct gatactgggc ctcgtggaag gtttgaccga attcctgccc
     3061 atttccagca caggacacct gatcctggcg ggagacctgc tcaacttcaa cgatgaccgt
     3121 ggcaaggcat tcgaaatcat catccaggcg ggcgcgattc tggcggtatg ctgggaatac
     3181 cgcgtccggt tgctggatgt cgtccgtcgg cttggccagc cggatgccaa ccgtttcgta
     3241 ttcaacctgt ttgtcgcgtt tctgccggcg gccatcctcg gcctgctgtt cgagcaccgt
     3301 atccagcaat atctgttcag cccgattcct gtggcgacgg cgttcattgt cggtgcgctg
     3361 gtgatcctgt gggcggaaaa gcgtgaccat gtggtgcgta tcgacagcgt ggacgacatg
     3421 cgctggaccg acgcgctcaa ggtcggcatt gcacagacct tcgccctgat tcccggcact
     3481 tcgcgttcgg gttcgaccat catcggtgcg ttgctgttcg gcctgtcgcg caaggcggcg
     3541 accgagtttt ccttctttct cgccatcccg acactgtttg ctgccaccgg ctacgaaacc
     3601 tggaaacacc gcgatatcgt gttccattcg gatggtaatc tgatgctgtt cggcgttggt
     3661 ttcgtggtct cttttctcag tgccctgatg gcagtgcgtg cgctgttgcg ctacatcagc
     3721 cgacacgatt tcacggtgtt cgcctggtat cgcattgcct ttggcattgt ggtgctggtg
     3781 acggcatggg gacactgggt ggactggacc cccaactgac cagccgtgac cggtcagccg
     3841 gtcacggggt caggcttcgc tgaactgccc ggtcagttca gcgattgtat ccagcagggt
     3901 atcctcctga tagggtttgc cgaggtaggc attcacgccc aggctcatcg catgttcgcg
     3961 gtgcttgtct gcggtacgcg acgtgatcat gatgatcgga aggtgtgccg tacggctgtc
     4021 ggcgcgcaat accttggtca gttcaaatcc gtccatgcgc ggcatctcga tatcgagcag
     4081 catgatgtcg ggttgtgtgt tcttgagcca ctgcagtgca tcgacaccgt cttttgcggt
     4141 gaccacatcg taaccctcgc gttgcagcag gcggctggtg atcttgcgca ccgtcagcga
     4201 gtcgtcgacc accatgacgg tgcgtttgtg cgcggccaca gccactggcg cagccggtgc
     4261 gggttcggtt gccggttcgt actgacggtg gcgcagctga accgggtcga ggatcagcac
     4321 gatctggccg ttgcccagta ccgtagcccc ggcgatgcca ggcacactgg ccagttgtgg
     4381 accgatgtgc ttgacgacga tttcctggtt gccggtcatg cggtcgacaa ggactgctgc
     4441 acgctggtta acgctgcgca gcagcaggac caggttgtag gcctgcagaa cgggctgccg
     4501 gtgcggatca cccagcaggt gcggcaagta ggcgaatggg tagttctggt tttgccagac
     4561 gatgtggccg gcctccatgt gttcgagcag ttcggccgac ttgaactcct gaacctgttc
     4621 aatcatgatt gacgggatgg cgtagacctg atcggtggca tataccagca ccgcctgcgt
     4681 cacggcgagc gtgagcggta ggtagattgt ggattgcagc cctttgccgt gggtcgattc
     4741 gagttcgatg cggccaccca ggcgggtgat ttcgttcttg accacatcca tgccgacacc
     4801 acggccggca agcgtcgtga ccgattccgc cgtggaaaag ccgctggcga agatcagttg
     4861 cgtcagctca tcctcgctcg gggtggcctc tgcggataac agtccattgc gaataccgcg
     4921 ttcgcgaatg gcggaaaaat caaggcctcg gccatcgtcg cggagcgtca gaatgagttc
     4981 gttgccctcc tgacgtgcag taagcacgat ctcgccgtat tctgacttgc ctgctgccag
     5041 ccggtcttcg gcggattcga taccgtgggc gaccgagttg cgcagcaggt gttctaccgg
     5101 cgcgacgatc ttgtcaagta cgctgcggtc gaattcgacc ttttcgccga ccagttgcag
     5161 gctggctttt ttgccgagat cctttgcggc gagccgtacc gtacggtgca gacgcaccga
     5221 aatgctcgac aggggcacca tgcggatgtg catgagcgac tgttgcaggt tcttggccag
     5281 ccggccttgt tgggtaatgg cggcatcggt ttcgttgagg ccgagcaaca ggttctgctg
     5341 cacggtctgg atgtcgttca cgctctccgc aatcatgcga gtgagttcct gcaggcgggt
     5401 gaagcgatcg aattcgagtg gatcgaactg ggcatcttca ttgcgcatgt gtgacagcgt
     5461 ggattgcatt tgcgattcgg cctgaatttc cagttcgcgc agctgggtgc gtagccgttc
     5521 ggcattttcc gtcagttcga gtgcgttgga gcgcaggttg ctggtactgc tttcgagtcg
     5581 tgaacgggtg atgttgattt cgccggcttc gttgaccaga cgatcgacgg tgtcggcgcg
     5641 tacccgtagc gcctgcgccg ccagttcgct gtccgagacc ggcatggcca cggcagcctg
     5701 ctgtgctgtc tggggtgcag caggtgttgc gctcactggt tgatccgttg cagctggcgc
     5761 ctgtggttcg gcggctggtg gattgcgcag tcgctcgacg gtcaggttga tctggtctag
     5821 cgcgctttcg aacgagtcaa aatcgatcgg caggtcatgg cgcaatgatg cctggcgcac
     5881 gatctcggtt tcgagttcgt gggtaagctc gccaagctgc atggcgccgg ccatccgcgc
     5941 actgcctttg agtgtatgca gcaggcgttg caactgcagt tcggattcgc ttgaaggtgt
     6001 gttgcgccag tcgcgcatcg cctgttcgat ggcgggcatc aggtcgcttg cttcttcgag
     6061 gaagatcggc agcagctgct cgtccacgtc gtcgtgcacc tgggattttt ctgtcgctgc
     6121 ggcttcgtcc agactggatc ttgcgggcat tgtcgcagtc tgcgagtttt ccgggcccat
     6181 gccttcggtc aggggatgcg tgcctgtctc tgtctcggcc agggtggttg cttcgtctgg
     6241 ccagtgggcg tccgtgatgt cgtccgagtc ttcggctgca gggggggtgt ccggtctggt
     6301 gctctgaccg gcgatgcgct gcaatgtttc agcgagctga gtttgcgcca ggggttgctg
     6361 gtgctcctga atctgctgct gcatgtgttg tagcgcatca agtgcggccc gcaccacagg
     6421 gtggtgttcg tcagctacag gctgattcga aaccagcggc gtcagatagg attcgagtgc
     6481 gtgcgacagt tcggcaatgg cgctgaaccc tgtgagccgc gcgattccag ccagcgtatg
     6541 cgctgcgcgc atgaaatcgt gatttaccgc ggtggcattg ctgttgccgc tgagggcgaa
     6601 ctcgctgttg agcgtgttca ggtggacctg agcttcttgc agaaagattt caagcagtgc
     6661 ggcggggacc gggttatcac caatggtgat caaggcttcg tcgtctgctg cctgatgggc
     6721 aggcgctgtt gtgtcagggg ttattgctgc aggttcgtac acggtttccg attcagccac
     6781 ggcttccgct tcagccgcga tttccggctc ggtcacggtt tccgcttcag ccacggcttc
     6841 cgcttcagcg gcgatttccg gctcggtcac ggcttccggt tcagccgtga tttccgcttc
     6901 agccacgatt tccggttcag ccacggcttc cggttcagcc gcgatttccg tttcggccac
     6961 ggcttccagt tcagccacgg tttcctgttc agccatggct tccggttcag ccgtgatttc
     7021 cgcttcagcc acggtttccg gctcggccgt gatttccgtt tcggccacgg cttccggttc
     7081 agccgcggtt tccggttcag ccacggtttc cggttcagcc gcggcttccg cttcagccgc
     7141 ggtttccgtt tcagccgcga tttccgtttc agtcgcggtt tccggttcag cagcggtttc
     7201 cgcttcagct acggcttccg gctcggccgc gatttccggt tcagccgcga tttccggttc
     7261 agccgcgatt tccggttcag ccgcgatttc cggttcagcc gcgatttccg gttcagccgc
     7321 ggtttccggt tcagccgcgg tttccggttc agctacggct tccggcccgg ccacggcttc
     7381 gtaccgggcg tgttcgccag tcagcagtgc ttcgcgcatg cggtcggcgt gtgcagcaag
     7441 ggctgcatgg tcgtttggat cgggctgggc gtcggccagc gcttcgatcc aggcgctgaa
     7501 gtgagagtgg gcgcgttgca gccattgctg caattcagcg ctggcggtgc gttcgtcctg
     7561 caaccacaga ttgagcagtt gctcggcggc ccaggcggtt tcacccagat cggtctggtt
     7621 taccatgcgg ccgctgcctt tgagcgtgtg aaagccgcga cggatttcgc gcagggcgtc
     7681 gatggcggca ggctgttcgg tcagggtgtt caggtggaca ctcatgtcgg caagcacttg
     7741 ccgcgcttct tcgatgaaga tcgatagcag gtcttcatcg tcgtcgcagg cgtgcatgtc
     7801 ctggctcgct gtgtcgacag gcaatggccg ccctgtgacg cgatcgatgc attcgagcgc
     7861 aagcggccag gccgagtcgt cctgcatgtg gtgcaaagcg gcatcggcct gagtgcgcag
     7921 gagtgggtcg gcgaccagtt ctgcatcacg ggccagttgt tgcaaaagct cagtcagttt
     7981 tttttttgcc gctgcgtcga tgtcctggct gcgtagtgca tcggtgcggg cgatgagttc
     8041 gttcttgatg gtctcgattt cgtcttcgac acgctcgcga ttgctgctgt cgccgctgtc
     8101 gggctggatc agtcccagtt gcaacaacac cggacgcaac acgttgatgg cattgggttg
     8161 ctggtagcgt tcggcatcga catacaagct gataccgctg atggcttcag cgatgagcgg
     8221 gaaagcagtt tcgtcaagat ggccggatga gagcatcatc tggcggatcc tgtcgcgcgt
     8281 gagttcgagc acccggttgg catcgtccca ctgcatgatg ctgagagcgc cgtgaatttc
     8341 ctgtaacggc ttgtccagtc cggtgatcag ttggcggtca tgggtgttgt cgcggaaaaa
     8401 cttgtcgagc gtttcctcga tctcgcgtac attgtgctgg atctcatgtg cgatctgcac
     8461 catgagctgt ttttcctgcg ccttgcgcga aatgtcatcg agcaacgata catcgctggc
     8521 gagacctgcg agatcggtgt cgctccaggc cgcggcctgc aaacgttccg tcattgcctg
     8581 gcactggcga tcgaggttgg gatcgatggt ttcgaaatgt tcgagcgtat tgtgtagcag
     8641 cagcagagcc gtggccattt caagcgtcag cggctccagt cggcgcggcg cgatggcgct
     8701 gtaatcgatg gcgatacggt ggatctcgct gatcagcctg gtcagagccg gttgctggag
     8761 ccggttgcca aggtcgtgca gttcgtcggc gtgtacctgg aaaaatgcga ggctgtcctg
     8821 attgccggac gacagccggt tccacgattc cttggcctgg ttgaccagcg ccaatgcctg
     8881 acgcaccgag tcgcgcagtt catcgctcgg tttgtcctgt gacgtggacc gaggcagcag
     8941 tgcgggcagc tggaacgcct gttttacggc ggcaatgcgt tcactgccat cgcggctgtg
     9001 ggctacgaag tagagcaggt cgcgcaacag gcgttcggca actttgctgg agccttcgat
     9061 gtgccggcgc agctgcaggt cgatgcggcc acacagttgc ttgacttcaa agccgggttc
     9121 gatggaacgg gtaatcaggc tgtcgacaaa ggcggttgct ccccaccaga acgtgcgcgc
     9181 ctgggatgaa ttcattgcgg cttcgacttg ttcgacggca ttgcgcattt cggtcaggcc
     9241 ggtttcggca tcgcgctgac gcagaaagga tagcaaacca cgctggaatt gcgctcgtgc
     9301 cttgcggtac agcatgacac gttgcggctc cgggatgttg ctggccctgg cgaaattcgg
     9361 cgcgcgggag tccatgtcgg gaaaaaacag gtcgctttcc tgggtacgcg tgatgccgcg
     9421 tgcctcgcgc atgctgcgat acacgggaaa cagcagcaat tcatggttgg gtttgccgcg
     9481 cagcagatcg tcgaggtaat ggtcgatttc gcgtgtggca gcgagtgcca gttccagatg
     9541 ttcgctgttt gcggcaattt caccgttttc catggcgttg accaggtgtt cggcttccag
     9601 gcaaacgatt gccacgccgc gcaggctcac catttccagt gcgccgacga cctggtgaag
     9661 gaaggtgcgc gccgaacgca gggcagcggg gtcttgtgtt gcgccctgga attggcgcag
     9721 aatttcgctc gcctcgtgca gtgcgtgact gacctcctca cgaatccagc ttaaaggggc
     9781 aatatcgaat tccggcgttg tgttcatggt catatccgta atcagtgacc gggcacactt
     9841 ccgagccggg ctcgggagcg tgcggggtgc gacgcaggcg ccagcatggc gtgatccgtt
     9901 atcccagctt gaagccggag atcgatttct tcaggtccga tgccagcgcg gacagttcgc
     9961 cgatggacga cgccgtttgc tgcgtacctg cggtggtctg ttcggtgatc tgccggatct
    10021 gctgcatggc ctgggcaacc ttggatgccg agacggtctg gttttccgtg gcggcagaaa
    10081 tgctgcggat cagtgtggca aggttgcgtg atacctggtc gatttcgtgc agcgcctgac
    10141 cggcagcatc cgacagccgc gcgccttcca ctaccccctg ggtactggtt tccatggcag
    10201 ataccgcatc ctgcgtgtca gactgaatgg tcttcacgat ggcggaaatc tgtttcgtcg
    10261 cctgcgcgga acgttctgcc agtcgctgca cctcttccgc aaccaccgag aagccgcgtc
    10321 cggcttcgcc agcggcggcg gcctggatgg cggcgttgag cgccagcacg ttggtttgtt
    10381 cggtaatgtc ggaaatcagt tccacgatct cgccgatttc ctgtgaggat tcgcccagtc
    10441 gcttgatgcg tttggaagtc tcctggatgt tttcccggat actgttcatg ccggcaatcg
    10501 aattttccac ggcgcgggcg ccttgttctg cggcttccag cgactgggtg gccacctgtg
    10561 ccgactggtt tgcgctggcc gatacctcgt tgatggattg cgaaatgccg aggatgtcgg
    10621 cagaggtgtt ttcaatttcc tgcgattgac gttgtgccgc agcgagcagc tcttccgaaa
    10681 tggcttgtgc ttgcagcgtg gtgccagtca cctgctccgt ggcatggttg atgcccgaga
    10741 cgaggttgcg tagctcgtcg atggtgaagt tgatcgaatc cgcgatggca ccggtaatgt
    10801 cttcggtgac ggtggcgttg gccgtcagat cgccatcggc gaggtcaccg agttcgttga
    10861 gcaggcgcag aatcgcttcc tgattgcgct tgttttccgc ttcgctgagg aacacacggc
    10921 gccgcgattc gttgaggatg acgaacccga acagtaccag gctggtcagc gccagcaggc
    10981 cggtgatgat ggccagggtg ttgcccagca cgcccagatt tttgtaggca ccggccagat
    11041 tctgtgtgga tacgtacagc gagtcgctgc tgcccagcag cggcaggctg ctgcgcttga
    11101 gcgtcaatac ctgtgatacc tgatccagcg tggtcggcaa ctgctcgtgc aacggtttcc
    11161 aggtggcttc aaacatcgcg agccggctgc gcgccgcgct gtccgaaaca ccggcttcgc
    11221 cgttctggct gctcccggaa cgcagtcgtt cggcaagtga ctgcacgctg tcgatatcgc
    11281 tgcgaatctg tttcaggatg gcctggtcct gtgccaggtt gctcttgctc atgcgccatt
    11341 ccggctggtc gacgaggcgg ctggcaatgg cggtgttctt gccgatgttt accgtcagac
    11401 cgaccagctg gttggccagt gccgcctggg cgggcgatcc gctgccgttc agtcggctgg
    11461 cgacatcccc gctcagcttg accagatcgc cgatctgctg caacagcatg ccgagctggc
    11521 tggatgtgtt ttccagcaaa gactggctgc tggcaatttt cagcaactga ctgttggtcg
    11581 gctgccattg tttctgcagt cctgcaagga tcggctgtac tgatgatggt gacggcggca
    11641 gacccagacc gccgttgatc agcacgtgca gatcctggtt gaactggtac tggctttcct
    11701 tcatcgcagt gaaggtgcca tggttacccg acagcgattg cagtgccgat ttgctcagac
    11761 gctgggtcag catctgcatt tcgcttgccc gggtgatgta gccggtcttg ttgctggtct
    11821 gcaccgcatt gagtgtaagc agcaggccgg tgatgaccag gaaggcgagg ccagagatca
    11881 tggtaaggat gtactgcttt tccacaggca ggtggccgat gaaaggaaca tccccgggcg
    11941 aactgctgga catttttttc agtccgctca tgatcagggt ggtgttttcc gccgttgcgg
    12001 ggcgcttgcc gccagatttc tggtcttcct tttttctgga gccggaaaaa ttgaatgcca
    12061 tgcctctatc tccactgata atccacccgg tttccgggag gaatttgtaa ttgtcgttct
    12121 tgggtcacgc cttgccggcg gcaaggaact ccgcatggtt gaccagactg tcgagatcga
    12181 cgttgtgcca caatgtgcct gcttcgtcgc ggtaacgttc gttgttccag cgtgcttgcg
    12241 ccacctcggg atcgcgttgc atctgttcca ggttacgcag gccgagggcc tggtcgacca
    12301 gcagcgcggc ctgggccgag aagcgggtgt gggtgagcag taaccggttg ccggcatgca
    12361 tgctggtggc gccaagtccg gcataggcgg gcaggtcgac cacagtgtac agacggccgc
    12421 ggatgttggt gatgccgagg aaccaggagt gggtgagtgg cacactggtg acgggcggag
    12481 gagcgatgac ttcgctgatg tcggccagat cgatgagcca gttctgtccg cctacctgga
    12541 cagccagccg cgtggtgccg ataggttgag cagacgaccg ctggatgcgt tcgcttagcg
    12601 cttgctggaa ttctctcagg ctgatgcgtt tggacatgcg cggttttccc tggacgcgat
    12661 tccgtctggt ttacaggttg cggatttttt ccagcaacgc attgccgtcg actggcttga
    12721 cgatgtattc gatggccccc tggcgcaatc cccagatctt gtcggtttcc tggtccttgg
    12781 tggtgcacag gatgaccggg atgtggcgca gttcttcgtc acgcgaaatg gcgcgcgtgg
    12841 cctggaagcc attgaggcca ggcatgacga catccatcag gatgaggtcg ggttgcgatt
    12901 gccgtgcttt ttccagtgcc tggttgccgt cttcggccag tgtgacctgg taaccatgct
    12961 tgcccagcag gctggcaagg aagaagcgtt cagtcggaga atcgtccaca acgaggattt
    13021 tctggatcgg catggccgga caccttgaag gattagtcgg tttgagcgct tgcgtaacgg
    13081 cgcacagcgg tcagcagtgt atctttggta aacggtttgg tcaggtattc gctggatccg
    13141 accatgcgcc cgcgcgcctt gtcgaacaga ccgtccttgc tcgacagcat gatgaccggt
    13201 gtggcactgt actgggggtt ctttttgatc agcgcgcagg tctggtagcc gtcgagacgc
    13261 ggcatcatga catcgacgaa aatcaggtcc ggatgcccgt cggtaatctt ggccagcgca
    13321 tcgaagccgt cttcggcaag aatgatgtcg catcccgcct gcttgaggaa aatctcggcg
    13381 ctgcggcgga tggtgttgct gtcgtcgatc accatcacct tgatgccatt cagctcggtt
    13441 tgctctgtca cattggcccc ggtcgtattt cccgcctggc gctcgcccga tggacggggc
    13501 attgccggat gcggatatat ataaaaacct gcgcatacat ttttaatctt gtttcatgcg
    13561 cttggcaagt attttttatg cagaaccgca actggttcca gtgttttcag tcgattgtaa
    13621 cgtcaatcag tccatttttt caaaatcttc cttgcgggca ccgcattcgg ggcaagtcca
    13681 gtttggtggc acatcttccc agcgggttcc gggtggaatg ccatcatcgg gcgcaccttc
    13741 tgcttcatgg tagatgtgac cgcacagtag gcagatatag ctggcataag gaatagaaga
    13801 catctttgtt tccggctttt cggttagaat ggttattcta atgcagaagc cgtcagatct
    13861 gacgcagttt tgaaatgacc cgtaccccat cgcccattgt cctgactttt tccgcttcgg
    13921 atcctaccgg aggggcaggc atgcaggctg atctgcttac cctgaccagc atgggctgcc
    13981 atgccctgac agtacttacc gcagtgaccg cgcaggatac ggtgggtgtg gaaaattact
    14041 ggtcgatcga ggctgatgcg atcatcgatc aggcgcgctg tgtcctcgag gacatgccgg
    14101 tggccgcctt caagattggc atgctgggca gtgtggcggc ggtggcagcg attgcgtcca
    14161 tcgtggctga ctatccggat gtcccgttgg tgtttgatcc tgtgctggcg tccggccgcg
    14221 gtgacgaact cggtgacgac gatacgcgtg ctgccatgat cgacatgctc gtcccccaga
    14281 cgacggtgtt gacccccaac agcatcgaag cgcgcagtct gtttgccgag atgcatgccg
    14341 gcgtagcagg ggtcggactg gaggaatgtg cccgggcact gcttgataca gggtgcgaat
    14401 atgtgctggt gaccggtacc catgagcaga ccagcgaggt cactaacgtg ctctatgaca
    14461 gtcagggcgt tgtgcgctcc gacagttggg cgcgtctgca tggcagttac cacggttccg
    14521 gttgcacgct ggcttccgcc attgcagcca cgctggcgca taacatgctg gttcccgagg
    14581 gcgtgttcga ggcgcaggaa ttcacttacg agacgttgcg agcggccttc cgtcctggca
    14641 tggggcagca tatccccgat cgcctgttct gggcgcgtca cggagagtcg cgatgatctc
    14701 gggattgtat gccatcacgc gcgaaacgga tgacaccgcg cggctgcttg ctcaggtcga
    14761 ggcggccctg tacggtggtg ccggtgtgat ccagtaccgc gacaaaagtg gggatgctgc
    14821 gcgccaacat gagcaggcga gtgaattgct gaatttgtgc caccgtttcg atgtgccgtt
    14881 gatcatcaac gacagcctgc gtctggccga tctggtcggc gcagatggtg tgcacctcgg
    14941 tcgggatgat ggcacggtcc gtgaggcacg catcatcctc gggcatgacc atatcattgg
    15001 cgtatcatgc tatcagtcgc tgccgcttgc actggcagcg caggaagccg gtgcggatta
    15061 cgtggcgttc gggcgcttct ttccttcggg caccaagccg ggggcccagc gggtcgagat
    15121 cgatctgctg taccgcgcgg cgcatgtgat tcaccggcca atcgtggcga tcggtggcat
    15181 cacgcctgac aacgcggccc agttgatcga tgctggtgcg gacgcggtgg ctgtcgtcgg
    15241 tgccctgttt gacagcgcgc agattgaaga gacggcgcgc cagtttgtgg atttgttcgt
    15301 agatgagacg gaagactgac atgaaccaga atgaactgtt gtttgcgcgc gcgcaacggc
    15361 atattccggg gggcgtgaat tctccggtac gtgccttccg ttcggtaggc ggtacgccaa
    15421 aattcattgc atcggcacat ggcgcacgga ttcgcgacgc ggatggcagg gaattcattg
    15481 attatgtcgg ttcatggggg ccgatgatcg ttgggcacgc ccatcctgac gtgatcgcgg
    15541 cagtgacccg ggtggcgcgg gacggattga gcttcggtgc gcctactgca gcggaagtgg
    15601 atctggccga attgcttgtc gaactggtgc ccagtctgga aatggtgcgg ctcaccagct
    15661 ccggtaccga agcgaccatg agcgcgttgc gcctggcgcg cggttttacc ggacgcgacc
    15721 gcatcgtcaa gtttgctggc tgttaccatg gccacggcga ttcgctgctg gtcaaggccg
    15781 gttcgggtgc gctgaccttt ggccagcccg attcctccgg tgtgccgggc gcgctcgccg
    15841 aactgacgat ggtgctcgaa tacaacgata tggccggtct ggaaacggca ttcctgcagc
    15901 agggcgagcg gatcgccgcg gtgatcatcg agccggtggt gggcaacatg aatctgatcg
    15961 cgccagctcc ggaattttta caggcattgc gcgaattgtg tacccggtat ggcgcattgc
    16021 tgattttcga tgaggtgatg acgggttttc gtgttgcgct gggcggagcg caggcgcatt
    16081 tcggcattgc tcccgacctg tcgacgttcg gcaaggtgat cggcggcggc atgcctatgg
    16141 cggctttcgg ggggcgtcgc gacgtcatga gcctgctggc accactcgga cccgtctacc
    16201 aggccggcac cctctcgggc aatccggtgg cggtggcggc aggactggcc acgctgcatc
    16261 tgctgcgcga gcccggattc tacgagcgcc tggcactgcg cacgcgccag ctgaccgagg
    16321 gattgtgcgc acgtgccgct gcgcacggtg tggtattttc tgctcagtcc gtcggtggca
    16381 tgttcggtat gtattttcgc gccacgccgc cacgcaatta ttccgaagcg cttgagtctg
    16441 accgtgagcg gttcaaccgc tttttccatg ccatgctgga tcaggggttt tatttcgcgc
    16501 cgtcggctta tgaagccggc tttgtttcca gcgcccacgg tgaagctgaa attgcagcca
    16561 cactggaggc tgccgatcag gcatttgcgg gtctggcctg agcggccccg ggcactgtcc
    16621 gatcaatacc gcgagggcat gcggattgga ttggtgatgc gttgcgcacc ccgggccggc
    16681 gcgcgtggcg ccccgcccgg gatcgtgggt cagagcgtgc cgtaagagtg caggccggag
    16741 aggaacatgt tcacaccgag gaaggcaaac acggtcacga acagtccaat gactgcccac
    16801 caggcgagaa atgcgccgcg cagtcccttg accaggcgaa tgtgcagcca ggcagcataa
    16861 ttgagccaaa cgatcagcgc ccaggtttcc ttgggatccc atgaccagta gccaccccag
    16921 gcttcggcag cccacatggc tccgaggatg gtggcgatgg tgaaaaagcc gaaaccgacc
    16981 gcgaccgact tgtacatcag gtcgtcaagc acgtcgagtg agggcaggcg cgaggcgagg
    17041 atgccacggt tcgccagcaa ccaggctact gccaccatgg cggcgagcga gaacgagccg
    17101 tacccgatga aattggccgg gacgtgcagt ttcatccagt aagattgcag tgcgggcacc
    17161 agcggctgga tattctgcgc atggcggtcg aaggtgtacc agagcaggaa gcccaccgcg
    17221 gcgctgatca cgggcagcac gaatgcgccg agtgcgcggg tagagtagcg cgtctcgtaa
    17281 tacagataga gcagcgaagt gatgatcgag aacaggatga acacttcgta caggttggag
    17341 atggggatgt gacccacgtc cgggctgatg aggtaggatt cgtaccagcg caccatcagg
    17401 ccgatcagcc ccatcgctac agcactccag gtcaggctgc tgccgacccg tgccgcgaag
    17461 tcggaacgaa tggcgagcga cagccagtag gtcagggtgg ccagcacgaa cagggtgctc
    17521 atccacatga tggcggactg gctggccaat atgtacttga gaaagaatgc gctttgcgcg
    17581 cgcgccagat tgccgtggta atcggaaatg ccgaacaggc tggcggctgc taccacaacc
    17641 agcagtacgc gtaccggttt ccagaaccag cccagtaccg aaaataccgc tgctccgccg
    17701 aagaggaacg acttttcata aatgtccatg tagtgaccgt actggctcca ggcataggcg
    17761 gcgccacccc atagcatcag ggcgtacagc cagtcgagca gtctcagttg gcgcaagttt
    17821 ggcaaggcag gcaaggtttg tgcagtctgc atgtcagggt tcctttgcaa tgacggcaat
    17881 acgttgttcg tgctgggtaa attcggcttc gaaatcgaga gtcggacgat tgctggacat
    17941 ggccagcagg atgcgatcgg gtttgatcag cagccacacg cggcgttcct ggatgaagaa
    18001 catggcgaac acacccagta ccagcaggaa cgagccgaaa tacacgacgt ttttgccggg
    18061 tgagcgtgtc agttgcagcc ctgaggcctg cacctgatcg tacgtggaca gttgcaagta
    18121 taccggggag ccatagaaga agatatcgct gattgtattc aggctgtcgc gcaggaacca
    18181 tcccgtcgca tcgtccatgg caggggccgg cgcatgctgt tcggtttcga tcatgttcca
    18241 tgcctgccag gcggagaact cgaggatctt gaggtaggct tcagcggccc ggtcacgttg
    18301 tgccgccggt acgttggctt cgatgaaatc ggtcagcgcc ttgaaaccgc cggtagagaa
    18361 catttccagc acccgttgcg tgttggctgc cagcgccttt tgcccggcgg gatcgtcggg
    18421 cagcacgctt gccgcgaaac ggcgcgacag ttcgggatac agtgccggat tgagcatggc
    18481 ggcgcgcagg cgcatgaagc catcaagcga cgagttggga tcgagcggga aacgcaggta
    18541 actgtagggc tggttgggtt cgctgcgcac gcccgacacc atgaaccagc ggccgtcgat
    18601 acgcactggc agcacatagt tgctgtactg gtgcgcctga ccgtccgcat tgcgtatttt
    18661 gtactggaaa ctcgggccga cattgtgcag gttgtggttg tcggcgacgg cattgccgcc
    18721 gaacagcggc ttggtcatgg tgctggccgg gctgttctcg ccggtgtctt cgatgttgaa
    18781 tgggtgaaag tcggtgaatt ccaccgtgag ttgccggtca ctgtcggtca ggcgcgtctt
    18841 gtcgtgcaca ctgccttcga cgggaaatgg cgtgcccttg gtgtcgagca ggttccagcc
    18901 gcgcaggtgc aggtgggtgc cgccgtcgcc gaagctggat tggtaaatgg cgataccgtc
    18961 atagatgagc gggtggttga cctcgatggt gcgggtgagc aagggctggc cagtgcgctt
    19021 gtcgaggatc tgcaggtcgc tggcgaaggt cttgggctgg ccggtggaat aatgttcgat
    19081 gtgaaacttg tccagcgcga tgcggaacgg cagttcctgc accatgtatc cgtcgccgac
    19141 attgatgaat atgatgtcgg cactgtcgcc ttcagggatg gtgacatcgc cgcggaacga
    19201 ccagttggag gtcgacagcc ggctcgcggg cgggacctgg ctttcgggaa tttcgcgggt
    19261 ttcaatgcgc ttgataccga acgcttgctg cagttgcagc ggcagattgc cgtcgatcag
    19321 tccgcccagg cagatgagta cgatggcggc gtgggtgaac aggtagccca tccggttggc
    19381 gctgccgcgc ttggcggcaa gcaatagccc ctgtgggctg gaggcttcgc ggtaacggta
    19441 gccctgggcc gtgaggtatg cgcgcagttg tgtcgatttg tccgcgctga ccggaaattc
    19501 ggcccggtgg tggaagtgga gcaatgattg ctcggtggcg tgttcgcgat agctacgcat
    19561 ttcgcgcagc atgcccggcg tgttgcgata aatgcacatg ccggtcgata gcaccagaaa
    19621 ggcgagaatg acgatgaacc accaggtgtg gtacacatca aacagcccca ggctcttgaa
    19681 gaccagaaac cagtactgac caaattcgaa cacatagtcg gaataaggct ggttctgttt
    19741 cagcacggtc ccgatcaccg atgcgatggc cagcagggtg aacagactga tggcgaaccg
    19801 cattgaactg aacagttcgt agagcgagcg acggatggtc gcgtgggctt ggctcatggg
    19861 ttagtgtgct tcctgaaatg aaagggcggc ttgcgccgcc ctcttgggta ccggccgttc
    19921 cctgcgggac ggtgccggct cgccagaccg ccagcaccgc gggcggtcgt gtgactggcg
    19981 gattactgca gaccggatac gtactccgct acggctttca tttcctgatc ggtcatgcgg
    20041 gcagcgatgg tgcgcatcat tttaccacca tcattggcgc gttgtccgag gcggaaattg
    20101 ttgagctggg tatacacgta ttcgctatgc tgtccggcca ggcgtgggaa ttcggcaggg
    20161 atgccggcgc cggtggggcc atggcaggcc atgcaggcag gtacgccgga tgcggcgtca
    20221 ccacccttga aaatcttctc gccggcttca gccagatcct tgtccttggc ggcgcccggc
    20281 ttcgtgatct gctcggagaa atatgcgccg agattcttca tgtcgtcatc ggtgaggttg
    20341 ctgctggcca cgataccctg catgatcggg ttggtgcgct tgccggattt gaaatccatc
    20401 agttgcttgg taatataggc ggcgccctga ccggccagtt ttggcttgtc aggagcggtg
    20461 ctgttaccat cggcgccgtg gcaggcggcg caaacggtcg atgccaactg ttgcgctttg
    20521 gccggatcgc ccttgcttgc cgtgtcctcg gcgtgagcga gggagaacgt ggtcaaagcc
    20581 gtgagtacca caaacgccat ggtctttttc aattgtaagc tcctctacct taaacatgcc
    20641 caaagccctc attctatccg tattttgatt tacgtgctag cctgataatg acatcgggat
    20701 ataaatttca tgaatttgtt tcgtactgca acatttcaca ccaccgtgaa tcatctgcgc
    20761 gatctgccgc aggacagtct gggcgaaatt gcctttgctg gccgctcaaa tgccggaaaa
    20821 tcgagcgcga tcaacacgct ggccaatcgc aaccgcctgg cctacgtgag caagacgcca
    20881 gggcgtactc agcacatcaa ttacttttcg ttgggtgaag gactgtatct tgttgatctg
    20941 ccggggtatg ggtttgcccg tgtgccgggc gcggtgaagg atcactggcg cggcctgctc
    21001 ggcgactatc tgcaaaaacg cgagccgctg ctgggcatca tgctgatcat ggatatccgt
    21061 cgcccgctga cggcgctgga tcaggcgatg ttcgactggt ttgccgtcac cggcaagccg
    21121 gtgcattgcc tgctgaccaa aagtgataaa atcagtcacg cggctgcggc gaaggcattg
    21181 cgggaggtgc gcgcatggct ggccatgcgg tcggatgcct acagtgcgca gctgttttcc
    21241 agcctgaaaa agtcgggtgt ggaagaggtc gtcgcagtgg cggacaactg ggtgcgtgat
    21301 gcacgcaacc gacaggcaga aaaaaacctc ggttaaaggg gaggataacc gaggtcaaag
    21361 tgtcctgact ggtgtcaaga cgcccgctca gggaggagaa gcgggagatg gcacggccat
    21421 ctgctggtta tgacgcaggc gtgtggcaaa agttccgaaa aatttgaaag gatgatcaat
    21481 gagggctcaa gcggcatttc ccggggtgcg tatgcgccgc atgcgcaggg acggattcag
    21541 tcgcaggctg atgcgggagc acagcctgtc ggccagcgat ctgatctacc cggtgttcgt
    21601 gcatgagggc gcggaccatg ctgtcgctgt gccgtccatg ccgggagttg agcgcatgtc
    21661 gctggaccgt ttgctggagc aggccaggcg ctgcgtggcg ctggggattc cggccatagc
    21721 cctgtttccg gtcatcgacg atgtgctcaa gtcgaccgat gcggtcgaag cgaccaatcc
    21781 ggacgggctg gtgccacgcg tggtgcgcgc catcaagcag gaatttcccg ggttgggtgt
    21841 gattactgac gtggcgcttg atccttacac cagtcacggg caggacgggt tgatcgatgc
    21901 gcagggttac gtgatgaatg atgacaccgt ggaagtgctg cgacagcagg cgctggcgca
    21961 tgcgctggcc ggtgcggatg tggtggcgcc atcggacatg atggatggcc gggtggcagc
    22021 gatccgtgag acgcttgacc agcacgggct gatccatacc cgcatcctgg cgtatgccgc
    22081 caaatacgcg tcgtcgtttt acgcgccgtt ccgtgatgcc gtcggctcgg cgatgagcct
    22141 gggggtggcc ggcaaggaga cctaccagat ggatccgggc aattcggatg aggcgttgcg
    22201 tgaagtcgcg ctcgatctgc aggaaggcgc cgacatggtg atggtcaagc ccggaatgcc
    22261 ctacctggac atcgtgcgcc gcgtcaagga cgagtttggc gtgccgacat tcgcctacca
    22321 ggtgagcggc gagtatgcca tgctcaaggc agcagtgggt aatggctggc tggacgagcg
    22381 caagtgtgtg ctcgaggcac tgctggcctt caaacgggct ggtgcagacg gtgtgctgac
    22441 ctatttcgcc atccaggcgg cggaatggct cgccgagggc tgaggccctg acgtgatatc
    22501 cgctgcgggc agatgtgccc gcagcgcttc atgccgatgc gctgtcggag gtttcctggg
    22561 caagcaggct ggccagttgt gtcatcgacg cacgccgcga cagttctcct ccggcgcggg
    22621 cggcgtggcg cacggcgtcc gccggccgta ccgatacgcg gatgtcgcat aacgttccgt
    22681 tcagaatgaa gcagggtacc ggtctgcgcc cgtgtctgtg gccgcaatcg gcctgacgaa
    22741 acgggatgtt gtggttgagc aggtacattt ccacgtcttt ttcattatcg gtgaacagtt
    22801 cgatgtcgat gtcggaatga cggccggcgc tgccgtcaag cacgctgccg gtcaggtagg
    22861 ggtcgaatcc ggacagcatg tgcatcgtct gcaacgcgct ggtgcgcaac tgccgcagcc
    22921 actgcgggtg cgtgtcggca tgaaacaccg catggtattc gcggatcgcg gcctcgattt
    22981 cctcattgcc cggcaggtgg tgcgtgtcga cgataccgag tttccgcgcg gctttgcgcc
    23041 tggcagcgga aaagtcgcgc atgccgtgct cacacatcaa ttgtgcggcc agttgtgcga
    23101 ccagttctcg cgtaggattg cgtttcatgg tcgtggcggc tgggggcgaa tgtctgttgc
    23161 aggcagcggc ggttgtgagc gcaagccttc gggcagcgct ggctgaggcg tgctggtgtt
    23221 gctaccggcc gctgccgcgg gttgggtcga cgccggcagc gacgatacgg gttgtaccga
    23281 gacggactgg gtcgacggaa cggcgtcctt gaagaaatat tccggtgtac cggcttctcc
    23341 tggtgcaacc ggcagcccgg tcagcggatt gatcatgacg ctgaccacgc cgtcgggagg
    23401 tgtgtagggc gtttgaggca cattgcgcag cgcgacggcc atgtatttca tccagattgg
    23461 cagtgcggcc tgggcaccgg tttcgccgtt gccgaggctg cgcggctgat cgaagccgat
    23521 ccacgctaca gcgacgagat ccggctggaa gccggcaaac cacgcatcgc gctggtcatt
    23581 ggtggtaccg gtcttgccgg ccaggtcgtt gcggccaagc tcggcggcct tggcgccggt
    23641 gccgcggcgc acaacatcct gcatcatgct ggtgatgatg aaggcattgc gcgggtcgat
    23701 gacccggcgt ggtgattccg gtttgcctgt ctggatgaca tggccatact ggtcggtaat
    23761 gtggtcaatg atgtacggtg tgacctgtga tcctccattt gcgaaaatgg cataaccgga
    23821 gaccatctgc aatggggtga ccgagaccgc ccccagtgcc atggtcaggt atggcggcac
    23881 atgttgcggg tcgaagccga acctgcgtac ataattctgc gcgtagtcgg ggccgatggt
    23941 ttgcagaatg cggatggtcg gcaggttgag cgaatgcgtc agcgcatcgc gcatgctgac
    24001 cagggtgccg ttccaggtgt cctcataatc cttgggttgc cacagtacgc cgccggtctg
    24061 ttccgccggg atggaaatgg gcgtgtcttc gagcagcgtg gcgggtgtga agcctttttc
    24121 cagtgcggca gaatagatga atggcttgaa gctggaaccc ggttgccgcc aggcctgggt
    24181 cacgtggttg aatttgtcca tgtcgtaatc gaacccgcct gtcagtgccc ggatggcccc
    24241 ggtgtgcgga tcaagcgaca ccagcgctgc ttcgacgcgc ggcagttgtg caatacgcca
    24301 tccgccctcg ggcatgcgct ggacgcggat cagcgatccg gggcgcagca gggccggatg
    24361 gccgtgcgat gggaccagtg cgcgtgcggc gagtcggagg ccttcgcccg agagggtggc
    24421 aagtccgtga ttgcgaaccc agacctgcag gcggtggttg tcggactgca acaccacggc
    24481 cggaagcagg tcgtgtaccg gcaggatgtc ctgcagtgtg gcatcaaggg cggattcctg
    24541 tcccggtggg ggcaggggca gcgaactttc cgggccgcga tagccatgac ggcgatcgta
    24601 ttcttccacg ccctgccaca gggcgtcgtt ggctgcgttc tggtcgcgct tgttcaacgt
    24661 ggtgtacacc ttcatgccgc tggtgtaaat ggcgtcgtgg tattgctcat acatggcctg
    24721 gcggatcatt tccgcaacat agtcggcatt gacggcatag tgttcgtcgg ctgtcttgcg
    24781 cacatgcagc ttttcggcca tcgccgcatc ataatctgcc tgggtgatgt agtgcagttc
    24841 aagcatgcgg tgcagcacgt agtgctggcg tgccagagcg cgtggcaggt ttactgccgg
    24901 attgtagcgt gaaggcgcct tgggcagtcc ggcgagcatg gcggcttctg caatgtcgat
    24961 gcgggcgagc ggtttgccga agtaggcctc ggctgccgcg gcaaagccat aggcgcgctg
    25021 gccgagatag atctggttga tgtacagcgt gaggatctgg tccttggtca ggttgcgttc
    25081 gatcttgatc gacaacaggg cttcattgag cttgcgtgtc caggtttttt cgcgcgtgag
    25141 aaagaaattg cgcgcaacct gcatggtgat ggtcgatgcg ccttcacggg cgccaccgga
    25201 caccaggttg ctcagggcgg cacgtatcac gcccatggca tcgatgccgc catgctgata
    25261 gaagcggtcg tcttccgcgg cgaggatggc atgctgcaag atgatcggag tctgcgccag
    25321 cgtcaccacc gcgcggcgct gttcaccgaa ttcggccagc agcgccccat ccgcggtgta
    25381 cacgcgcatg ggcagtttcg gccgatagtc agtgagcgct tgcatgtccg gcaggttcgg
    25441 ccagatgagc gatacggtca gtgcgcccag cgcgagtgca agtacgacaa gaccggtaag
    25501 ggttgcaaac agagcggctt gccagcgcga tgtgaacatg aagcgtgatc catatgtaga
    25561 ggtgggatga gcctcgggaa ttataacagt gtctgcggtg ccggggcgga tatgaaacca
    25621 ggcatgccgg ccatttatct gtccccgtgg agccttcgca gaaaattcat gcggcgtcca
    25681 cattgagaaa caaaaaaaat agtttacact tgcgaatgtt gctgatagtc tttaacgtga
    25741 attattggtt tgtttcctaa ccatcaaatt aaaaaaggaa tcctgttgca attcaacctt
    25801 ttcaaaatca agacacctcc tctggtcggg gtcgatatca gttcgacagc agtcaagctg
    25861 gttgaattgg gagaagccgc acggggacgg tacagtattg aacgctacgt catcgagaac
    25921 ctgccaaaag atgccgtggt agaaggcaat attgccaata tggatgaagt cgtcgatgcg
    25981 ctgaagctgg cctggtcgca attgggcacc cgttcgcggc acatcgcgat ggcgctgccg
    26041 tccgcttccg tgatctcgaa gaagatcacc ttgccagccg ggctgagcga ggatgaaatg
    26101 gaaacccagg tgcaggcgga ggccaaccag gtggtgccat tctcgctgga cgaggtcaat
    26161 ctcgatttcc aggtgctggg gcagggtgca tcgcccggcg gggatgtcga ggtgctgctg
    26221 gtggcgagcc gcaaggaaaa gatcgaagat cgtgtggcgg ctgccgaagc cgctggcctc
    26281 aaggtgctgg tcatggacac ggagtccaat gccagccaga ccgcgttcga gggcatggtg
    26341 cacgtgcttc cggacagtgg ccggggccag acggtggcat tgatcgacat tggcgcgacc
    26401 acgatgcaca ttctggtctt tgtcgataat gtcatggtgt attcgcgtga acagtcgttt
    26461 ggcggcaacc agttgacgca ggaaatccag cgacgatacg gcatgaccta cgaagaggcg
    26521 gaaaaactca aacgcaaggg cgagatgccg gaccgttatg gtctcgaaat cctgcaaccg
    26581 tttacggaaa cgctggcgct ggaaattggc cgcgcactgc agatgttcgt cgcatccact
    26641 tcctggacca aggtggacta catcgtgctt gcgggcggta ccgccattct gcccggactc
    26701 gacgaggctg tggcggggcg cactgaagcc agcaccatgg tggcgaatcc gtttgccggc
    26761 atggcgcgtt ccgaacgtgt ggcagctgac cggctggcga tggacgcccc ggcgctgctg
    26821 gtggcgtgcg ggctggcgat gaggaggttc gactcgttat gatacgcatc aacctgctcc
    26881 cataccgcga gatgcgccgg gtcgcaagac tgcggcagtt ttccctgttg ctgggcgcct
    26941 cggcgctggc tgctgcaggc gttgtggtgc tggtgtatct ggcgctcgaa gcgcagatat
    27001 acaaccagaa ttcccgcaac gaatatctca agtcgcagat caccctgctt gacaagcaga
    27061 tcagcgacat tcatgaactc aagacacaga ccagggatct gctggcgcgc aagcaggtgg
    27121 tcgaaaccct gcagatgaac cggtccgatg acgtgcatct gctcgatcag ctggtgcggc
    27181 tcatgccgga gggagtgtac ctcaagtcga tcgaccagca cggcgccaac gtgcatctgt
    27241 cgggctatgc ccagtcaagc gcacgcgtat cgacactgat gcgcaatctt gattcgtcgc
    27301 cgtggctgaa gaatgcgacg ttgatcgagg tcaagtcggc cagcgtgcag aatatccagg
    27361 ccaacgaatt cagcctggat gtgcagatgg tttcgcctga ccagagcgaa agtgataccg
    27421 cgcatacggg aagcggcgca ggacatcacg cgggcggaaa ggcgggttga acgatggatc
    27481 tgaatgaact gaacaacatc aatctccagg atctggccaa cgctcctgct ggcatacgtt
    27541 cgggcatcct ggccggagtg ttcgtgctga cgcttgcgtt gggttactgg ctgctgctga
    27601 gtggccagct gggtacgctg aatgcggcgc gccaacagga acagacgctc aaggatacgt
    27661 ttctggagaa gaagcgccag gcgatcaacc tcgatgccta tcgccagcaa ctggttgaaa
    27721 tcaatcattc gttcggtgca ttgttgcggc aactgcctaa ccggtcacag atggacgccc
    27781 tgctgagcga tatcaaccag gcaggactgg gccgtggcct caggttcgat ctgttcaagc
    27841 ccggaaccga gaccatgaag gacttctatg ccgtgctgcc gatcagcatc cgtgtgagcg
    27901 ggtcatatca tgacttcggt gcttttgcga gcgatgtggc gcaactgtcg cgcattgcca
    27961 cgctgcgcga catcagcatc gcgcccgaca aggatgggca actggtgatg aatgcgacca
    28021 ttgaaacgta ccgctacctg gacgaggatg agttgtccga acagaagcgt ctggcagata
    28081 aaaacagcag gaaaggggca aaatgaagcg tgtcttgcgt attgtcccac tgatgctgct
    28141 ggccggttgc catatgggcg ggcaggatga cctccaccag ttcgtgcagg atgccggtgc
    28201 gcacatgcgc ggccgggttg aaccgctgcc cgaagtcaag ccgtatcagc cgttcgtcta
    28261 caatgatttt gatcagtccg atccgttcaa gccacgcaaa ctcaagttca attcggctga
    28321 aggtggcatt cagccggacc tcaatcgtcc gaaggagctg ctggagtctt ttgcgctcga
    28381 aacgctgaaa atggtcggcg tgatccagcg aaacggcgtg aattatgcgg tgatcaagac
    28441 tccgctcaat accatttacg ttgtcaaggt cggcaattat ctcggacaga attttggcaa
    28501 gatcgtggcg attaccgaca aggaagtcaa attgaaagaa atcgtgcagg atggtgcggg
    28561 tgactggtcc gaacgggaca gctccctgaa cttgcaggaa caatgaggag tgttcaaagc
    28621 atgaaaaccg caatcgttcg aatgggacaa cacgtgctga tggcgctgtt cctgctggcc
    28681 gccctgacaa cccagcatgc gcatgccgat accaatgcca tatcgaatat caactttctg
    28741 ccgcagcaaa atggcaaact ggtgatgaag gtggacttcc agcaaaacct gcgcgcgacg
    28801 ccggcgagct tcaccgtcaa tacgccgcca cgcatcgcgt ttgacctgtt caacacatcc
    28861 aatggcacgg gcaagaatac tctgatggtc aatcagggat tgctgcattc ggtcaatgtg
    28921 gtgcaagccg gcgatcgtac gcgtatcgtg ctcaacctgc tgcagccggc gcattacacc
    28981 atgcgtatcg acggcaattc gttgctggtc cagatcgacg aggccggtgc agggtccgtt
    29041 accaatgaaa ccacccattt tgctaccggg gggccgggtt ccgacaagca cgtcatccgt
    29101 gatgtcgatt tccgccgagg taacggcggc tccggacgtg tcatcacgac gctgtcagac
    29161 agcaccaccg gtatcgacat ccgcaagcag ggggatgcgc tcattgtcaa cttcatgaac
    29221 accgatctgc cgcaaaacct gcagcgccgg ctggatgtca cggatttcgg cacgccggtc
    29281 agcagcattg aaactgtcgg ccgatccggc atggtcacca tgaccatcaa accgaagggc
    29341 gagtacgatt attctgccta ccagaccgac aatcagttca ttgtcgaagt gcgcaccaag
    29401 accgaggcaa atgcgcaaca ggatggcaag ccacattaca ccggcgagaa gttgtcgctt
    29461 aatttccaga acaccgatgt gcgctcggtg ctgcaggtga tcgccgactt taccggcaag
    29521 aacatcatca ccagcgatac cgtgaccggt aacttgacac tgttgctcaa ggatgtgccg
    29581 tgggatcagg cgctcgatat cattctggag agcaagggtc tcgacaagcg cgaaaacggc
    29641 aacgtgatct gggtcgcgcc caaggatgag ctgctggcca aggaaaagag cgaactggag
    29701 tcacgccaga gcatccaggc actcgaaccg ctcgtggtgc gccagtacca gctcaactac
    29761 aaaaaagccg accaggcagc gcgctcgctg ctcgggctgc caccgctgcc cggcgatact
    29821 ggccaggatg tgacctgcga tgcccaggct caggggatca aggcgcagac ggcagctgcc
    29881 gcaacggcca tgccgatggc caatacggcc aacaatccca atcgcatcct gtcgccgcgc
    29941 ggcacagcca catcggaaat gcagaccaat acactgattg tcagtgatac ggtggaaaag
    30001 caggaagaaa tcgcgcaact gctcaagctg attgatacgc cggcacgtca ggtgatgatc
    30061 gaggcgcggg tggtggtggc tgatgacgag ttcagcaagc agctgggaac caagttcggg
    30121 gtgtcgacgc cgttcaccac cagcaatggc ggcaccctgg gagcactgtc gtccacggga
    30181 accaatgcca acacgctggt caatggcagc acagctctgt ccaatgcggt ggccaacggg
    30241 ccgatgtaca acgtcaacct gccgtcggct gcgaccaatg ccgcgacgat gggcctgtcg
    30301 ctctacaatc tcggcagtgg cgcgctcatc aatctggaat tgtcggctct tgaggctgac
    30361 ggccgcggca agatcatttc cagcccgcgt gtgatcaccg ccaaccagaa accggccgtg
    30421 atcctgcaag gtacgcagat tccgaacgtc acgccaggta cggcaaattc gccaccgctg
    30481 gtgaccttcg tcgacgcgtt cctgtgcctg ctggtcaacc cgcaaattct caacaacgac
    30541 tcggtgatcc tgaccatcga agtgcaggac gatgctccgg gaacaccgtt tagcagcggt
    30601 ggtgtgattg cgacgccaat cgataccaag cgtgtgaaaa cggaagtgcg cgtgaaaaat
    30661 ggcgagaccg tggttctggg tggcatttac taccagaaca agcgtaagga cgtaaacaag
    30721 gtgccggggc tgggtgatct gccgcttatc ggcgggctgt tccacgataa ttcgaccgtg
    30781 gacaacaaga ccgagctgat ggtgtttatc acaccgcgca tcgtcaagga cgacatgacc
    30841 ctgcagtgag catgcgactg cagtgcgggc gctgccgtga acggcatcgc ccgcccggat
    30901 gtcggcagga gcctgaatga tcgcggacaa gcacatcata ctggttgggc tgatggggtc
    30961 tggcaagtcc accgtgggta agctgctcgc tcgccagatg catctgccgt ttcatgattc
    31021 tgatcaggaa atcgtgcgcc gcaccggtgt caccattccc atgattttcg aagtcgaggg
    31081 cgaagccggt tttcgccgac gggaagaaca ggtgcttgcc gaactgctgc aacgtgaacc
    31141 gcaggtgctc gccaccggtg gcgggattgt gctgagcgag atgaaccgtg cgctgatccg
    31201 ggagcacggc acggtgctgt atctgcgcgc gaccgtggat gaactgtggc aacggacccg
    31261 gcacgacaag aaccgcccgc tgctgcaggt ggacgacccg cgccagcgtt tgcagcagct
    31321 gtttaccgac cgcgatccgc tgtatcgcga agtggccgac atcacggtcg acaccggacg
    31381 acagtcggtg cagtccctgg tacaccagct ggtgcgccag ctgggtcggc aaaaagtctc
    31441 atcctaggga cgcggcggtt cattccgcgc accgcgttgc tgaacacatc gtgatgatcg
    31501 tgagccggat gacgcaggtc gtggccgtga ccacggtgcc agggcatccg gtaaaacgtg
    31561 cgcacgatgt ttacgcaacc cgcatgagac gtggtctggc gtacggcgtg ctgcgttgca
    31621 tttcttgtca cgggggtcag ccattgactg cggattgcgc cttgcaatcc atcccgccag
    31681 accgactctg cgatcatgcg gatttaatca ccgcgtccct ggctgtgtcg gggtcgaggt
    31741 tggcgcagtc ggcagaccca tgacaacata cccggcgcgt gtattgaaat gccggcaaaa
    31801 ggtcagagcg ttccagcagc atcccatgcg catgcagtct ggcgaggtgc gccccttaca
    31861 gcaacacccg ttcgataccg ccctggttga cctcgcgcag atagtcgttc atccagttct
    31921 cgcccagaag atggcgtgca agttcgatga cgatgtaatc agtgtcggta ttggttgcat
    31981 cgccgtaacg tgataatccc tgcaggcatg acgggcatga agtcaggatt ttcaccgggg
    32041 gctgctggcc gttctcgccg cgcaaggcgt cggtgccctt gcggatttct tcttccttgc
    32101 ggaaacgtac ctgggtcgcg atgtcgggcc gcgcaacggc aaacgtaccc gattcaccac
    32161 agcaacgatc ggacagcgcc accggctcac ccatgagctg gcccaccact gtcagtggcg
    32221 catgtgattt catgggcgtg tgacatggat cgtggtacag gtagcgcgtg ccggtcgcct
    32281 ggggcagatg cacgcctttt tccatcaggt attcgtggat gtcgagcagg cggcaaccgg
    32341 ggaatatctt gtcgaactgg tattgcagca actggtccat acaggtgccg cacgacacca
    32401 gtaccgtctt gatgtcgagg tagttcaggg tgttggcaac acggtgaaac agtacccggt
    32461 tggcggtaat gatggcatcg ccgcgatccg cttcgccgct cgatgtctgc gggtagccgc
    32521 agcacaggta gcccggcggc agcacggtct gcgtacccag cgagtacagc atggcctgcg
    32581 tggccagacc gatctgcgaa aacaggcgtt cggaaccgca gccgggaaaa taaaacacgg
    32641 cgtccgaatc ttcgctggta cgtgacggat tgcgcagcac cggtacaatg cgcgcgtctt
    32701 ccagcccgag cagcgcccgg ctggtcttgc tcggcacgtt ggctggcagc ggcttgttga
    32761 cgaagtggat cacctgcgcc ttgaccgatg gcgagcccag tgtggccggt ggatgggccg
    32821 tctgggccgc gccgatgccc agccgtttga acagccggta gcccagacgc tgggcggcat
    32881 agccccattc gatcatgact ttgtgcacca tgcgaatggt ggccggatca gtggcgttga
    32941 gaaaaaacat gcccgccgcg gtgccggggt tgaatcgttt cttgccctgc ttgcgcagga
    33001 aattgcgcat ggcgaccgat acgtcgccaa aatcgatgtc aaccgggcaa ggattgacgc
    33061 agcggtgaca gaccgtgcaa tggtcggcca catcgccaaa ttcgctgaaa tgctgcagcg
    33121 agacgccgcg ccgggtttgt tcctcgtaga ggaacgcctc gatgagcagc gatgtcgcga
    33181 gaatcttgtt tcgtggcgaa tacagcaggt tggcgcgcgg tacatgcgtg ctgcatacgg
    33241 gtttgcactt gccgcaacgc aggcaggatt tgatcgaatc ggcgatgttg ccgatttcgg
    33301 acttttccat gatcagcgat tcggtttcca gcagtgaaaa gctcggcgtg taggcattgc
    33361 gcaggtcggc accgggcatg agcttgccgc ggttgaaatg tccgtgcgga tcgacctgct
    33421 gcttgtagcg cacgaagtgc ccgatggttt cgggggcgag aaattccagc ttggtgatgc
    33481 cgatgccgtg ctcgccggaa atcacgccgc ccagttccgt ggccagctgc atgatgcggg
    33541 caacagcggc gttggccgcc tgcagcatgt cataatcgtc ggaattgacc ggaatgttgg
    33601 tgtgcacatt gccgtcgccg gcatgcatgt gcagcgcgac gaacacgcgg ctgcgcagta
    33661 cttcgcggtg aatcgcgtcg cagcgctcga gtacggcctg gtattcgcgg ccggtgaaga
    33721 tctgtgccat ctcgtggcga atctcgcgtt tccacgacac gcgcagcgtg tggtcctgca
    33781 gcgcgtgaaa cagcgtcgta tgccgcgcgc cgtcgaggga ttgcatcagg tggccggaat
    33841 cgggcaactt cccgaccgga acatccatgt gggtgagcag ccacttccag cgcagccggg
    33901 ttccatcaat gcattgcaag gcacgctggc gccgttcttc tgtcatctgc gggctgggtt
    33961 gcagttcacg ctcggcttcg aatggcagct cgccgcgcag gaactgctcc agcgccgtca
    34021 gcatgtcgag cttgttgcgg atcgacagtt cgatgttgat gtgctcgaca ccatcggaat
    34081 aatcggccag ccgttcgagc gggatgacga catcttcgtt gattttgaag gcattggtgt
    34141 gtgcagcaat ggcggccgtg cgtgcccggt cgagccagaa tttcttgcgt gcggtcggcg
    34201 tggtggcgat gaagccttct ccgccgcgcg cattggccag gcgcacaatc gcagacgcgg
    34261 cctcgccgca ggcaagatca tcgtcggaca cgatgtccgc cagcagaacc atgttgggtg
    34321 cctgctgccg gttggccttg gtggcgtaac ctacggcgcg caggtagcgg aagtcgaggt
    34381 gttcgaggcc ggccagcaac gctttgccat gattcgaaag gtaatcgcgg atttcgacga
    34441 tggccggcac agcatcgcgc acctgaccga aaaattcgag acagaccgta cggatgtggt
    34501 tgggcatgcg gtgcagcaca aagcgcgccg aagtgatgat gccgtcgcaa ccttcctttt
    34561 gcacgccggg caatccggca aggaatttgt cggtgacgtc cttgcccagg ccggttcggc
    34621 ggaagtgcgc tccaggaatg agcaactgtt cgggttcgcc gatgggcgtg ctgccgtcag
    34681 cggcatagcg gcgcaattcg aaccccactt cttcctggtc atggatcttg ccgcaattgt
    34741 gattcagccg ggtgacttca agccagttcg cgtccggtgt gaccatgcgc caggacacca
    34801 ggttatcgag cgtggtgccc cacagcaccg cttttttacc cccggcattc atggcgatgt
    34861 taccgccaat gcacgaggag tcggccgaag tggggtcgac ggcaaatacc agtccggcgg
    34921 catcagcggt ttcgatgacc cgttgggtga cgacaccggc accacagtgc acagtcgggt
    34981 agaccacatc cgtgcctggc agacgaacgt tgttcaccgt tcccagggca tccagttttt
    35041 cggtgttga
//
LOCUS       DAIANL010000059         6930 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1496137, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000059 DAIANL010000000
VERSION     DAIANL010000059.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496137"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1425 a         2246 c         1974 g         1285 t
ORIGIN      
        1 tcccgtggcg aaaatggctt ggtgatgtaa tcgtcggcac ccgtttccag accgagcacc
       61 ttgtcgcgtt cgtcgccacg cgccgtcagc atgatgatcg gcacgttctt catgcgcgcg
      121 tccgcacgga agatgcgcgc cagctccatg ccactcatgc cgggcagcat ccagtcgagc
      181 agcaccagat caggcagtga ttcgcgcgtg agcatcagtg cctgctccgc cgagtcggcg
      241 cacattaccg tatgcccgcc ctgggtcagg ttgaacttga ccagctcctg aatgccagac
      301 tcatcttcga ccaacaatat cgtcgccgcc atcggtaact ccatttgatt ggcttatact
      361 tacacctgcc atgtacgtac ggccggggcc gacacaggca tgcgcggcta ccggtcttcc
      421 ggcacaaata ccggcatatc ttatgacaac aaaatgacaa aataatgacg aatggtttat
      481 tttgcaaacg aaatgttgtg gaaacgtcat gctgacgctg ggaatcgaat cgtcctgcga
      541 tgaaaccgga atcggtctgt atgacagcga acacgggttg ctggcgcatg ccctgcacag
      601 ccagatcgcc atgcacgccg aatacggcgg cgtcgtaccg gaactggcat cacgcgacca
      661 cctgcgccgg gtggtgccgc tgatccggac cacgctggcg caggcggagc ggacgctacg
      721 cgacatcgac gccattgcat ataccgaagg acctggcctc gccggcgcgc tgctggtcgg
      781 gaccggcatt gcccatgcgc tgggcatggc gctcgacgtc ccggtgctgg gtgtgcatca
      841 tctggaaggc cacctgctct cgccactgct gtcgcacgac cgaccggaat ttccgtttat
      901 tgcgctactg gtttccggag gccataccca gcttatggcc gtcgacggta tcggccagta
      961 ccgcctgctc ggcgatacgc tcgatgacgc cgccggcgaa gccttcgaca aaagcgcgca
     1021 actgctcgga ctgggctatc cgggcggcgc agccctgtcc gtcctggccg ggcaaggcga
     1081 cccgcaacgc ttcgtcctgc cgcgacccat gctgcactcc ggcgacctga atttcagctt
     1141 cagcgggctc aagactgcag tactgaacct gacgcgcaag cacccgctcg ccgaggcacg
     1201 tgccgacatc gcggctgcgt ttcaggccgc catggtcgat gtactgacca ccaagacagt
     1261 ccacgcgctg cgcagggcca aatgccgccg tctcgtggtg gccggcggcg tcggtgccaa
     1321 ccggcagttg cgtgagcgcc tgcgcaccga ggtcgaacgc atgggggcta cagtgcattt
     1381 tccgcaactg gaattctgca ccgacaacgg tgcaatgatc gcctttgccg gcgcgatgcg
     1441 cctgcaacac ggcggcaaca cagccggtgc gttcagcgtg cgtgcccgct ggccgctcga
     1501 cagtctcgaa gcaccgatac cgtgacgcac cgcatcctcc gcccgccagc acccggcgac
     1561 cgcatgtcac gctgcctgtt gcacacggta tgacccgatt taccgcaccc gcctgccagc
     1621 ctgccgctcc agtaccgcat cgaatgcatc gtagatgcgc tgaacgtagc ggttcttgaa
     1681 catcagactg gcctgttcgc gttcgggatg caccagcatc gtggcattgg cttcaaacag
     1741 cagcacacgg ccgtcaggca gcagcgatac gtcggcgccg gcaaaatcca gattcatgcg
     1801 ctgaccgatc gcggccatgg ctgccatgcc acgctcgccg aatactgcct gcgggtcttc
     1861 gaggaaacgc tgttcctcct cgagcttcca gtccaccagc atgccggcgg tggcatagtg
     1921 caccatccat tgcggcgaaa ttgccagatg ataaggaaac accttgccat caacgaacac
     1981 cagccggtat ttacgataaa aaccatccct ggagcggtag tcgtgccacg ccgtcaggta
     2041 aaactcccgc cctggccagt ccgtacgcaa gcgattgagg tcttccgcgc tgtcgacgcg
     2101 cagcagccct tcgccgccat gcgaacccgc cggccgcgcc agaaccggcc attgcacgtc
     2161 gagtggtggc gcggcatcgg ccgccagccg ccacacgcgg ggaatcacca gtccggggat
     2221 gccgtcgaac agttcctggc tctggtcgcg cgcagtacgt gccaccgccg ccggcagatt
     2281 gagcaccggt acgcggcaat gacgcaggaa agcgtccacc tgtgcggtga agcggctggt
     2341 catgtccggc tcgcccatgg cgttgaacac caggtcatgg tcgggcagcg acggatgcgc
     2401 cgggtcggca tcatactcga cgatccactc gatagtattg ttgcgctggc gcggaaacag
     2461 gtgctcgaac ggcacattac cgcgttcgct gcccagcagg atcagcaccg tgcgttccac
     2521 accatagcga tgctcgtgga accagcactt ctgccggtaa gcggtatcca tatactgtcg
     2581 tgcctgcgcc agccgaccgt cgttggcgag aatcgcagcc agattacggt tggcggtgat
     2641 gtgctgcgga cgcatgtcga gtactcggcg gtaccagatt tcagcgtcct ccgtgcgttt
     2701 ccacagcgaa tacaagatgg ccaggttgca caacgcctca acggcctgcg ggcgcaggcg
     2761 gatggcttcg agataacagg tttcggcaag ctcgtaacgc tccatctcct tgagcgcatt
     2821 gccaaggttg ttgtgtgcct ccgcatattc gggcttgagc cggatagcct cgcggtaacc
     2881 ggtgagtgcg gcctcgaaca tgcgccggtt atgcagcgtg ctggccagat tgaactgcag
     2941 ttcggcgcag tcgggctgga gcgcgagcgc ggtacggtac agctccatgg cttcgtcgat
     3001 cccgcccgcg gcatgcaggg cacaaccgag attgttcagc acttccaccg aagccggttg
     3061 caacgcaagt gcgtgtttcc acgcggcaat ggcctcggct tcgttgcccc gctgctgcca
     3121 gagcgcgccc agattgcacc aggcatcgaa gtgctcgggg tggataaaca cggcacgctg
     3181 atagcactcc atcgcctctt cggtcttgcc cattgccttg agcacgttgc cgagattgtt
     3241 ctgcatgccc gggttataag gccgcagcgc aatggcggca cgataacagc gttcagcctc
     3301 ttccagctgt ccgagatcct tgagtacgtt gccgagattg ttgtgtgcct cggcaaaccc
     3361 cgactggctt gccagcgccc ggcggtaaca ggcggcggct tcttccagtt ggccgagctg
     3421 ttgcagcgca ctgccgaggt tgcccagcgc ctcgggataa tccggccgca attcgagggc
     3481 gcgacggtaa tgcaccacag cttcatcaat cgctgcggtt tcctgcagca ggttggccag
     3541 attgtagtga atgaccggtt cgtccagcag cgcaagcgcc tcgcggtaac tcgccagcgc
     3601 ctccgtgagc ctgccctggt ccttgagcac gttgccgagg ttgttatgcg cttcagcatg
     3661 gtccggccgc gcggcgatgg cctgacgata gctgaattct gctgcttcgg gcttgccgag
     3721 ctgctgtaac actacgccgt aatcgacaca gtagaccgcg ttaccggggc agtgacgcac
     3781 tgcctgttcc atcatctccg cagcacgccc cagatcaccg cttgcctgcc aggccagacc
     3841 atacagatgc aacgcatcgg catgagccgg ttcgcgttcg agcacctggg cgtacttgcg
     3901 cctggcctcg tccagccggc ctgcacggtg gtatgcaagt gcagactgaa gcatgccttc
     3961 ggcggatacc gacggcgcag caggatgcga ctgccggccg ggcatgcagc agaacttgta
     4021 tttcttgcca ctgccacagg ggcaggtgtc gttacgtccg ggcttgaccg tcatgatccg
     4081 tttcctttcc gtgcgcccga accggcagaa acgcccggta caaatagttc cgaaaagact
     4141 ttacagggct tcatatttca ttggaaaatc aagcgatgcc gcgctggaag aggagttcgc
     4201 aatgttgcgg ttgatagcaa tcatatcaac aaatacgttt ccccgcatca ccgcagaccg
     4261 cgtctgcacc cggatgccct tgctgcgcga cccggcacgg ctgcccgcca ccggctccag
     4321 gtacagactg tagaacccat ggatagttca gaaaaattgg aaacaactga agcaacctgt
     4381 ttgcgcaccg cggaccgggt ggactatgcc gaactcaacg agcaggaaaa aagccagatc
     4441 ctgacactgc aacaatcgat cctcgaatcc atgctgcagg acaacagtcc gttgcagatc
     4501 atcaacaacc tgtgtcgcct cgaagagcaa ctgctaccca attcagtcag cagcgtcatg
     4561 ctctatgaca gcgcgcgcaa gaagctcgac gtgctggccg caccgagcat tccgcccgaa
     4621 gcacgcaaac agttttccga cctgcagcca ggaccgggat ccggttcgtg cggcaacgtc
     4681 atctaccagc agacgccgat cttcgtcagc aacaccctgg ccgactcgcg ctggctcgat
     4741 ctgcgtcatc tggcacaggc attcaacctg atggcctgct ggtcgatgcc gattcgcggc
     4801 gaaggcggac gcatcgtcgg caccttcgcc ctgtcgagct tcgaggcgcg cgcgcccagc
     4861 acattccacc gcaagctgct cgagatcggc gcgtcgatcg tcggcatcgt gctcgaccgg
     4921 cgtgagcaga tcgaatcgcg acgcctgtat tcacacattt tcgacagcag cggcgaaagc
     4981 atcgtcatca ccgaccgttc gcaggtcatt ctgtcggtca accccacgtt tacccgcatg
     5041 ttcggttacc cggccgagga agtcattggc cggccacccg gcttcctcaa atcgggacga
     5101 cacggcgcca gctactacca gaaaatgtgg accgaactgc accagaacgg ctactggcag
     5161 gacgaaatct ggaaccgccg catggacggc acgattcacc cgtacctgct cggaatctcg
     5221 ctggtgcgcg acgccgatgg cgccatcgtc cattacgtcg gcatctactc ggatatcggg
     5281 cgctacaagc aggcgcaggc acagatcgag tacctggcct accacgactc gctgacgcaa
     5341 ctgcccaacc gcctgctggt gcgcgaccgc gccgaactcg ccatcctgac ggcacgcgac
     5401 aacgctacca acctggcgct gctgatgatc gacatcgatc atttcaagaa catcaacgac
     5461 tcgctcggcc accgtaccgg cgaccagctg attctggaaa tttcgcaccg gctgcaacgt
     5521 tgcctgcgtg acaccgacac cctgagccgc catggcggcg acgaattcct catcgtgctg
     5581 ccgctgatgc cggacaacga tccgattacg cagctcctcg acaacatcat ggacagcatg
     5641 gccgccccgt tccagctcgg tgacagcgag atcaccgctt cgctgtccat cggcgtggct
     5701 gtctacccga acgacggcca ggacttcgac acgctgctca aggctgccga catcgccatg
     5761 taccgcgcca aggacagcgg acgcaatgca tggcgattct acaccgagca aatgaacgat
     5821 gatgcacaga accgcatcac gctgtgcaac ggactgcgtc aggccctgtc gcaccagcag
     5881 tttcacctgc attaccaacc ccagatcaac ctgacggatg gcatgatcgt cggcgccgag
     5941 gcgctgctgc gctggcaaca tccggtactg ggcaacattc cgcccaaccg tttcattccg
     6001 gtggcagaag acaacggcat catcatccag atcggcgagt gggtgctcaa cgaagcatgc
     6061 cggcaactgg tgcagtggca gcacgccggc ctgcctcgct tccgcatggc ggtcaatctg
     6121 tccgccatcc agttcacgcg cagcgacctg gtggcttcgg tcaaacgcgc actggaacag
     6181 tccggactgg acgcggaact gctcgaactc gaactgaccg aatccatcct gatgcacaac
     6241 agcgagcaga ttctcgccac ggtcaagcag ctcaaggctc ttggcgtaca actatccatc
     6301 gacgacttcg gtaccggcta ttcgagcctg tcctacctca agcgtttcaa tgtcgacaag
     6361 ctcaagatcg accagtcatt cgtgtacgac atggtgaaca ataccagcga cacggccatc
     6421 gtgcgcgcca tcatccagat ggccaaagga ctcaacctga ccaccatcgc cgaaggggtc
     6481 gaacacgcag aacaactgtc cctgttgctg gacgaacaat gcgatgaagc ccaggggtat
     6541 cacatcgccc gaccgttgcc acccgacgag tttcttgcct ggtgcaaacg tcaaccctgc
     6601 tgcggcacgt aaccgcgcac ctcaacgcgc ccggcctgac aaccggccgt cagggacaga
     6661 acgcctaaca ggccgttgaa aaaggtagcg aggacgcccg gatgcaaggc gcgagaaatg
     6721 caacgacgag acatatcaaa tcgataggcg tgggagtgag tatcgcagca acgccgccag
     6781 acgggtgctg cagtactttt tcaacggcct gctcatatgg tcgcaaaagt caataaatcc
     6841 tgatcgaccc tgggatgcga agtctggcta cgacgatgaa tcatgctcat atccgtgggt
     6901 tggttaccac atttcatcga tccgtcgcgg
//
LOCUS       DAIANL010000060        22656 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2512567, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000060 DAIANL010000000
VERSION     DAIANL010000060.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 22656)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22656)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22656
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2512567"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4535 a         6833 c         6854 g         4434 t
ORIGIN      
        1 agcagaatcg cctggcgatg ttgctagccg tgctgcaccg tcatgcgggc attgcctgct
       61 atgaccagga tgtgttcatc aacgcggtcg gcggggtacg catcaacgag ccagctgccg
      121 acctccccct gcttgcggcc atcgtatcct cgttcaggaa caagccggcc ggcgagaagc
      181 tggtgctgtt cggcgaagtc ggcctcaccg gcgaaatccg tccggtccag cgcggccagg
      241 agcgcctgaa agaagcagcc aagctcggat ttacccaggc gctggtgcca caggccaacc
      301 tgcccaggca gccgatcccc ggcctgacgg tcaccggcgt tgccgatctg cgtggcgcga
      361 tcgatttttt gcgcgagacc ggttgaggcg ttacgccgga tgtgaatcgt aagttcaggc
      421 ccgcccgtcg gcgtgcgctc atgcgctgaa gaatgcgacc gcctcagcga tttcccggct
      481 gcgccgcatc ggtggcaagc tttgccagat gcgccgcccg taaggcttgt cgaccagtct
      541 cggatcacaa atcatcagca ccccacggtc ggcctcgtcg cgtatcagac gacctgcccc
      601 ctgcttgagc gtgatgactg cgtagggcaa ctggtagtcc atgaacggat tgccaccctg
      661 tttgcgaatg tgttcgatac gggccgccag taccggatcg tcaggcgggg aaaacggcag
      721 gcggtcgatg acgaccagag acagggcctc accgcgcacg tcgaccccct cccagaaact
      781 ctggctgccc agcagtaccg gatgtgcgag cgtacggaat tgtgtcagca gttctccgcg
      841 cggggcgtca ccctgcatca gcagcgggaa atccagtccg cgctcggcca gccgcgttcg
      901 cagcagcgtc gtggcctcac gcatggcgcg caatgaagtg aacagcagaa aggcacgtcc
      961 gccacttgct tcgagcaacg gcagtgccgc atcgatgacg gcggcggtat atccggacga
     1021 attcggttcc ggcatgccgg ccggcacata cagcagcgac tgccgggcat agtcgaacgg
     1081 gctgtcccag caggcagtct ctgccgtgtt cagcccaagc tggctgcaat aatggctgaa
     1141 attaccgcgc accgacagcg tggcggaggt gaagatccag ctgcgggcgt gctgccccag
     1201 ctggctggca aacagctcgg ccacagacag tggcgtgctg ttgagcatca cggcgtggct
     1261 gaatacttcg agccagcgga tggcatcggt cggtgtcgcg cccagccagc gctgcagctg
     1321 ctgtgcctgt gccaggccac gctgccagag ctgttcgagt tcgggggcac gcggtgcatg
     1381 cggttccagc tgcgcgttga acagatcgag cgccttgccg cactgttcca gcgcctgcat
     1441 ggcagtgcgg tcggaacgca gccgttcctg attccagcgc tgtgcgtctt cgcgaaaggc
     1501 gaggcgaaaa tcgcgcgcgc tcttgtccag tgcacgagcg gcctgggtga cgccagggct
     1561 gtccttgcag tggatggcag ccccgatctg cgtatcgcgc gccagagcga tccattgcga
     1621 actcgactgc gattcgccga agaacagacc tgccgtctcc ggcaactggt gcgcctcatc
     1681 aaaaatcact gtattgcagg cgggcaacag ttccgccaca ccttcttcgc gcagccagac
     1741 gtcggcaaaa aacagatggt gattcaccac cacgatgtcc gcatccattg cccgtttgcg
     1801 cgcctcgaac acgaagcagt ccttgaattg cgggcaatcg ctgccgaggc aatcttcccg
     1861 ggtggaaacc acatgcggcc agatcggcga atcttccggc acgccgctga tctccgcccg
     1921 atcgcccgag cggctgtgct cggcgaaact gcggatgagc tgcaggtggt gcgcggtctc
     1981 acgctgctga aagcgagctt cttcactggt tcgttgcagg tgatggtggc agatgtaatt
     2041 ggcgcgcccc ttgagcagcg cgaccgtcgc tggtgaagcc agtgcagcgc gtgccgcggg
     2101 caggtcgcga tgaaacagct ggtcctgcaa agtgcgcgtg ccggtggaca ggatcacttt
     2161 cccgccgctg cgcagtgccg gaatcaggta agccagcgtc ttgccggtgc cggtgcctgc
     2221 ctcgaccacc agcacgctgc gctgctccat ggcggcttcg acacggctgg ccatctcgat
     2281 ttgttgcgca cgcgtgcgaa aaccgggcag ggcgcgtgcc agcgggccat cttcgctgaa
     2341 caggtgttcg atctcactca tgccgggagg gatcgatgtc gcggaaatgc acctgaatcg
     2401 ccagcatcga cagactgatg aatgcgccaa acaggaaagt gatgagggtg atcgacaacc
     2461 cggcgcgatc gagtatgcca tacaggccga gcatgatcag gatcgacaga ttctcgttga
     2521 aattctgcac cgcgatcgaa tggcccgccc ccataagcag gtgaccgcgg tgctgaagca
     2581 gggcattcat cggcaccacg aaaaatccac cgagaatgcc gatcagtaac aacaggatcg
     2641 cggccagcag cgggctggtg accacgctga tgctcatcac caccacaccc atgatgatac
     2701 cggtggaaat gacgcgcacc gagtcggtca gccggacgaa cttgccggcg atgaccgccc
     2761 ccagcgcaat gccgatggcc accacggcag tcagctggga cgcctgctgg atgccgaaat
     2821 gcagcatgac cagcgcccag gcaagcacga ccagcctcag ggtggcacct gccccccaga
     2881 acagggtggt gacggcgagc gacacctggc caagcggatc gcgccacaac tgacggaaac
     2941 tgcgccagaa atcgacgagc aacgccattg ggtcgcggct gggcggcgga tgatcgggtt
     3001 cagtggccgg aatgaacagg ttaaggaccg ctgcgcctat gtacagcatg ccgatcagca
     3061 gtacggccag tctggcgata tcgccggcga ccggccacgg caggttgtgc gccagcgtgt
     3121 gggtaatgtg cggatcgagc agtacgccgc cgagaaaggc gccgaggatg atcgccatca
     3181 cggtggcgcc ttccatccac gcattggccg caaccagttg ttccggggcc agatactcgg
     3241 taagaatgcc gtatttcgcc ggcgaatatg ctgcggcacc aaggccgacg atgccgtagg
     3301 caaacagcgg gctgacgccg aacagcatcg cagcgcaacc gaccagtttg aggttgttgc
     3361 tgatgaacat gacgcgcccc ttggggatcg catcggcaaa cgcgccgaca aacggcgcca
     3421 gtacaatgta ggaaacgacg aaaaactgtt gcagtaccgg tgtgtaccag ttggctgcat
     3481 gcatttcgcg caacaacccg atggcggcaa agagcaatgc attgtctgcc agcgcggaca
     3541 agaactgcgc cgacaggatg atgtaaaacc cggaattcat tttggctgag tgtcgcggtc
     3601 cgtcgtgctg ttttccgtgc gcgtaccacg tgccagttca cgcagcatgg cttcctgctg
     3661 cgcgatggat ggagcccggc tggtactgtt gaatgccggg tctgcacctg gcaacacatg
     3721 gccaccgcgt tcgcggttct tgacgctgat ccagtcatcg agtccggtgt cggcgctctc
     3781 atccgtcatt cggccgcgaa aaccccgtgg cgccgtcgat gggcgcgaac ggaacgcctg
     3841 caggcgcaac tgtttgttca ggctggcgac gcgatcgtcg atccagcgca ggtcatacag
     3901 tgcggccagc gtcagcagca ccagcagtcc gccaccgatg cagggacggc gcagcacgaa
     3961 ataggcgatc agcgcagcgg caaacacacc gcggtacagc catgcgccca gcggatgccg
     4021 caggtagtcg cgatgaagtc cgagcgggaa ggcaagcagg cgaacgaatg cctgcttgcg
     4081 gttacgcagc tgggccgcaa gttcccggtt gacctcctgg aggcctgcac ccccgagatc
     4141 gagttgtttc catgcatcgc tcatggtgat tgtcatccat actgcgcggc ccgagccgca
     4201 actgccgccg gcgcggattt tgtgttatgt tcggaactcc tggtggcttc gggtagccga
     4261 agcccataca ttctacacct ttgcgccggc cttgtgcccg cgaaccaacc ggccggcgcc
     4321 atgctgccgg ttgtccctga ccaatactga ccggaatatt catgaaacgt ttcatgctcg
     4381 tccaacattc ctattccgaa tttctcggca tggtggaaaa acagctcgaa tcgcgcaaca
     4441 tcggattcca gtaccagcgg ccattcctgg gcaatgacct gcccgccagc gccagtcagc
     4501 acgatgcgct gtggctgctg ggtggtgcct ttccggcaaa tgatgaactg gcgtgcccct
     4561 ggttaccgca ggaacgcaag ctgattgcca atttccgaaa aatggggcgt ccgatcgtcg
     4621 gcatcggttt cggcgcattg ctgctggtgg aacaggccgg tggcgaaatt cttgatgaac
     4681 cctatttcaa cacctactgg accgtctgtc acaagacgga agccggacgt gacgatccgc
     4741 tcgccagcgc cgtcgatggc cagcgcatgc tggtcatggt caacggcaat gctacattgc
     4801 cgcccggcat ggagccgctg gtggtcgacg acgccggccg ctggctcgca gtgcggccgg
     4861 atgagacgag ctatgccatg ctgttccgtc ccgaactcaa acccggcatg atcgaggaca
     4921 tgatcatgga ggacaaccgc ccgctgccgg acaacattgg cgagattctc gaccaggcac
     4981 gtgcggcgtg gggaacgggc cagaaagtgg cggcagacat ctgttttgcg ctggtatcgg
     5041 cactcgacct gatgaccgag cggcacaaga tgcccgtgtt cagcctcaat cccgtgggag
     5101 ccggcaatga agcgcaatga gcgggaattg ctggaactgt tccagcagct cgatgaaaca
     5161 cgccaggcag gactgattga ctatgcgctg tttctcgtcg aacgcgccag tggtgctggc
     5221 gagcgggaag tggccttgcc gctcgacatc ccgcggccgg aaaacgagtc ggtggtcaag
     5281 gccatcaagc gcctgcgcag ccagtatccg atgctcgaag ccggcaagct gctcaacgac
     5341 acctccaacc agatgacacg ccacatgatt catggcgttc cggcacacga ggtgatcgac
     5401 gaactcgaac agctgttccg caaccattac gacgcgttcg tacagcgttt ccatgcgcgt
     5461 gactgaacac gagatcacga ttcacggaag gcgaggcgct tgaccaccca gccgctgctt
     5521 gccgcaaccc tgttctgcac cacgctggcc gccgtgatcg tccggccatt cggattgggc
     5581 agtggcaccc ttgccatggc cggtgcgctg gcggcattgg cgagcggcgt cgtggcatac
     5641 agcgatattc cactggtctg gcagatcgtg caggacgcca cgttcacgct ggtgtcgctg
     5701 atcgtgatct cgctggtact ggacgccgcc gggctgtttg aatgggcagc gttgcacatc
     5761 gtgcacatgg cgcgtgggca tggcgtacgc ctgtttctgt tgctcaacgt attgggcgcg
     5821 ctcattgcag cgctgttcac caatgatggt gcagtgctga tcctgacccc attgctggtc
     5881 agtgtatcgc gcaatctgcg ctttccgacc ccggtcgtgc tcgccatcgt gctttcgggt
     5941 ggatttatcg ccgacagcgc cagcctgccg ctggtgacat cgaacctggt caacctgatc
     6001 gccgcacacc atgccggtat cggtttcgcc gattatgccc gcatcatggt cccggtggat
     6061 ctggtgtcgg tgctggcctc gctggtggtg ctgacgctgt attaccaccg tcaactgccg
     6121 ccacgccatg aacttgcggc tctgcctgcg ccacgccagg cgattcgcga tccactgatc
     6181 ttccgcgctg catggctggt gatgccggca ctgatcgtag gctacctggt ttcggcacgc
     6241 tggcagattc cggccagcgc cattgctgcg ccggctgcat tgttcatgct tgtgctggcg
     6301 gcccgcgacc atttcatacg caaacagccg acggtcgtca tccccttgct gccattgctg
     6361 cgcgaggcgc cgtggcaggt ggtgattttt tcgctcggca tgtacctggt ggtgtatggg
     6421 ctcggcaagc agggctggac aacctggctt gcagtgctgc tggcacatct gcatgatctc
     6481 gccccgtggc aggcggcatg gctcaccggc atgctggccg cactgctgtc gtcgctgatg
     6541 aacaatctgc cggcggtact gctgaccagc ctggccatcg cacaacagca tgtgccggac
     6601 agcgtgcgac agggcatgtt gctggccaat gtcaccggtt gcgatctggg gccgaaaatc
     6661 accccgatcg gcagcctcgc cacattgttg tggatgcatc tgctgcagca gcgcggcatt
     6721 catgtcggtt ggggggcata tttccgcacc ggggcagtgc tgacgctgcc ggtgctaacc
     6781 gtgacgctgc ttgtcctggg cagcctgatt tagcagaccg ttgcgcatca cgcgcccggt
     6841 ttgtgccgtg ccgaatatgc gcaaacattc atgaagaact tttaaaaagg tattttatca
     6901 attgtttttt ggatttattc cgttgattcc tgcggttcga gcgacccgga caagaagggt
     6961 gggtgacacg gcacccggtt tccggggcag cgcgttcacg gggctgccag catcgacgca
     7021 atggatattt ttggctgcca gtccagaaca tcgcgagcac ggctgcaatc gagggtcaga
     7081 ctgtgctgcg caccgtcgaa ccagccggtc tcgccgccaa taccgctcag gcgccacagc
     7141 cagcgcagac cgaatgcggc aacgcctggc ggaatgccca atgcgtgcgg tcggtgggcc
     7201 acgatcgcgt cacgcaggct gaaactaccg ggtgctgcca gattgcacgc gccttcgaac
     7261 ggttgcgtca aggcaagcag aatggcacgc gccacatcct gttcatgaac gcactgcaat
     7321 tgcggctggg gatcggggaa caccggatag accggcaggc gcaggattgc atgcagcaag
     7381 ggttgagcat gcggaccaag aatgatatgc ggccgcagcc gcatgatgct cggccagcgc
     7441 tgttccagcc agtgttcgat ctcggtcttg tgttcggcat agcagaagcc cggcaggggc
     7501 gtcagcggcg ccgtttcagt cagcatttcg ccatgaccat acaccgaggc gctggacaga
     7561 tgaatgatgc gcggcagacc agcttgcgcg gcggcagaca gccagtgttt cgaagcttcg
     7621 atattgattg cgcgcatgcg cgcaagcgcc atatgcccgc gcaatacgac ccaggccagg
     7681 tgtatgaatg cgtcgtggcc cgacaaccag tcctgcggat cgctgcgggt aagatcgatt
     7741 tgtcgcgcga ccagtttcgg gtgctgccat ccggtcggac gcagatcgat tccggtcacg
     7801 tgcgcgactg atgcgtgttc gcacagcatc ggcagcaaag cttgcgccag gtggctcgcc
     7861 gacccggtga ccagtacgcg catcccggaa aatgcggcag ccgtcatgcc agcgccggca
     7921 tcacgtcttc tgcaagacag tgcatcgatt cggcaaccag ttccggggca agggcgccag
     7981 caccgactgc gcacagcaga tgggtgacgc ccgcgtcgcg gtaggcctgc aaccgctcga
     8041 tgcattgcgc gggcgagccg accaccgcca tccccagcgt ctccagcaga cgaagatgga
     8101 tgcccagccg cagcagatgg cggaaacgtc cgacatcatg aaaatgttcg taactggggt
     8161 agagtttttc cagtaccggc agtgtcgtgg caaacagttg gcggtagaac cagacgaatg
     8221 ccggctcggc cagttcgcgt gcccggcgtc cgtcgggcag gcagaaaatg cccaccgtca
     8281 tgccggcgcg cggcggttgc ggtgcctgcc agtgctccag atactgctgg atatcgtggc
     8341 gcaccatcca ccacggtttg aatggaccgg ccagcacgcc catctgctgt cgcgcagccc
     8401 agatgaacgt gtcgggactc accgccgccg tccacagcgg tggatgcgga tgttgcaccg
     8461 ggcgcggcac cacttccagg ccgtcgaagc tgaaatgccc gccatggtgg cttagcggac
     8521 cgccttgcca gtgctggcgc agaatgtcga gggattcgtc cgtcagactg cggctgtgcg
     8581 ccatctctac gccgaacgca ttgaattcct gcggggaaaa cccgcggccg acacccactt
     8641 ccagccggcc gttgctcagc aggtccagtg tggccacacg ctcggcgacc tgcagcgggt
     8701 ggtggtaggg cagtggagtg acgcccagcc caagacgcag ccgctgcgtg cgctggctga
     8761 ctgcggcaag caacaggtcg ggcgctgacg aatgcgagta ctgcggcatg aaatgatgct
     8821 cgaccagcca caggcagccg aaaccgagct gatccgccaa cgccgcctga tcgagaaaat
     8881 cgtcaaacgc gcctgcttca tcctgatgca gttgcggcgg cagggaaatt tcgtggaaca
     8941 ggtcgaatcg catcccggca tgatgccatc aaagcgctgg ccgcggcaaa tccgtgccgc
     9001 gaacaggaac acaacagctg ccacacgcca tttgtcgttt ccgcctgctt ccagttagaa
     9061 tagcgctgtt tccggttacc tgatcctccc gtgcccgttg ataccctggt cgacatccga
     9121 aatgtcacgt ttgcctatcg caatcgcgcc gtgctgcagg acatcacttt gtccataccg
     9181 cgcggcaagg tcgtcgccat catgggcggc agcggttgtg gcaaaaccac gctgctgcgt
     9241 ctgaacggcg gccagttgcg tccaacccgc ggccgggtgg aagtcaacgg cgaagccgtg
     9301 cacgaactcg acaacgacgc gctgtaccac ttgcgccgca agatgggcat gctgttccag
     9361 ttcggcgcgc tgttcaccga tcttacggtg ttcgagaatg tggccttcca gatgcgcgag
     9421 catacacacc tgtccgaatc gatgatccgt gacctggtgt tgatgaaact caacgccgtc
     9481 ggcctgcgtg gtgcgcacca cctcatgccg ttcgagttgt cgggcggcat ggcacgacgg
     9541 gcggcgctgg cgcgcggcat cgcgctcgac ccgatgctga tgatgtatga tgaaccgttt
     9601 accggactgg acccgatttc catgggcgtg atcggtaacc tgatccgccg gctcaacgac
     9661 gcgcttggac tgacttccat tctggtcacg cacgacatcg aggaatcgct caagatcgtg
     9721 gattacgtct atttcatttc cagcggcaag gtcgaggccg aaggcacgcc cgagcagatc
     9781 cgtgcctcgc gtcagccatt cgtccatcag tttgtccatg ctgaagaaga cgggccggtg
     9841 ccgtttcatt accctgctgc ggattttgcc accgatctcg gcctgggcaa ggttgtccag
     9901 ccatgaagcc caacatcgtc agccgtctcg gcgcacgtac gctgaacgcc atcggccggc
     9961 tgggcacggc tgcgcgcttc ctgctgcaaa ttctctggcg ttcgggaacc agttggcgtc
    10021 gtttccatct catcattcgg gaaatttatg ccagcggtgt actctcgctg atcatcattc
    10081 tcgtctcggg actgttcgtc ggcatggtat tggggttgca gggctaccag acgttgcaga
    10141 cctacggctc ggcgcaatcg cttggcgtgc tggtcgctct gtcactggtg cgcgaactgg
    10201 gcccggtcgt ggctgcgctg ctgttcgcca gccgggccgg atcggcaatt accgccgaag
    10261 tcgggttgat gaaaaccacc gaacagttgc aggccatgga aatgatggcc gtcgatccgg
    10321 tggcacgcat cgtggcgccg cgcttctggg gaggggtcat ttccatgccg tttctggcgg
    10381 cgatattctc catgaccgga atttacggcg gttacatggt cggtgtgcgc ctgctgggcg
    10441 ttgatgatgg cgcgttctgg gcgcagatgc aatcggcggt ggatttccgt gaagacgtgc
    10501 tcaacggcgt gatcaagagc gtggtgttcg gttttgccat cagcaccatc gccctgttcg
    10561 aggggtatga tgccaccccg accgcagaag gcatttcacg cgccaccaca cgcacggtcg
    10621 taacctcgtc gctggcgatt cttgcgctgg acttcgtact gacggcattc atgttcaagg
    10681 gatcataacg aatggataga gttgcgctgg atttctgggt cgggctgttt gttgctgcgg
    10741 ggcttgccgc cctgtttgcg cttgccctca aggtcagcaa tgccgagagt ttcggcagtg
    10801 gaccggtcta cagcgtgcat gccagtttcg acaacatcgg cggtctgaaa gtgcgtgcgc
    10861 cggtgaagag tgccggggtg gtggtggggc gggtgagtga catcagctat gacaacacca
    10921 cattcgaggc cgtcgtaacc atgaagctcg atgaccgcta tcatttcccc agggacactt
    10981 ccgccgcaat catgacttcc gggctgcttg gcgaacagta tctcgcgctg gagccgggtg
    11041 gagacacccg aaacctcgcc aacggcgaca agatcaagat cacccagtcg gccgtggtac
    11101 tggaaaacct gatcagccag tttttgtaca acaaggcctc tgacgcggga ggaacgtctt
    11161 ccgccggcaa ggcatccgga gcaagccaat gaacaaactg ttacgctttt cgctgctggc
    11221 gttggggatg gcaaccctgc ccgcgctggc gcaggcccag cagcccatga ccctcgacca
    11281 ggccatcacg ctgtgtctga atgccgaccc gagcatcaag gagaagcagg aactcgtcga
    11341 gtcggcacgt gccatgctcg atgaagtcac aggtcacaaa ggtttgatgg tcagcgccaa
    11401 cctcttcgag ggactggcgc cggaggtcaa tggcgggttc tatcaggggg gcgcgttttc
    11461 cggcacccag cctcgtaccg atggccccta tccgggcggc ctgtcggact ggacttcact
    11521 tcaattcgcc atcatcaagc cgctgtatac ctttggcaaa gtggaagcct acagcgatgc
    11581 ggcgcagggc aatatcgaca tcaagcgcca ggacgtccgg ctgcgccaga tcgaaatggt
    11641 caagaaagtc aaacgtgcct actacagcta cctcacagca cgtgatctgc accggttgct
    11701 ggaagatgtg cagaaccacc tgaacgatgc gttgacgctg gtcaagaaaa acctcgcaga
    11761 cgacaatggc cagtcgcgcc agtccgacct gtatgcattg cagaccgcag aaggcatgct
    11821 gcagaagtat ctggcgcagg cggccgcagt ggaaaaaatc agcatggacg ggctcaagat
    11881 cctgaccggg accggcatgg accatccgct tgaagtggcc gatgacagcc tgacagccgt
    11941 cccgctgccc agcggcacat taaccgacta ccagaaccag gcgcttgctg atcgtcccga
    12001 aatggcgcag ctggaagcgg gtatgcgggc acggcgcgca ctggtgcagg caaaaaaagc
    12061 ggatatgtat cccaacctct atgccggcgt gatcggtgag gccaattatg cctccaaccg
    12121 caccatgctc aacaatccgt tcatttatga cccgttcaac aacgccggtc tcacgcccgt
    12181 catcggcatc gaatggaata tggaaccgag cgtgctcgca gcgcagcgcg cgggacagga
    12241 agccgaactc gaagcgctca atttcaaggc acagtatgcc caggccggca ttccgttcga
    12301 agtggccgag gcatggaatc acctgcaggc tgattatgaa tcccaactgc agctggccaa
    12361 aggggcagca gcggggcggc gctggatgat ttccgaactg gccgatttcg aagccgggct
    12421 ggaaaaggcg gacaaggtgg ccgatgcact gaagagctat gcactgacac aggcggaata
    12481 cctgcagacg gtcaacgact acaacatgga tgtggctgaa ctggctgttg tgaccggcac
    12541 attcaaatga cgcccgccgg tgtggccggc ggatggacaa catgacgccg gcagggctgg
    12601 cggacacagg cgaaaaggaa ttttcatgac aacattctct ctcatgcgac gcttgctgct
    12661 gcttgcgaca ctgtgcgctc cgctgctggc ccgggctgaa aacccgccac cggacgtgct
    12721 ggtgcgcaac accacgatcg aagtcaccac cattctcaaa cgcgacaagg ccgacctgag
    12781 caacaaccgg cagaagctgt atgccctgat cgacgccaag gtgctgccgc acttcgattt
    12841 caaccagatg acccaactgg ctgtcggtcg ttactggcgc caggccaatg ccacccagca
    12901 gcgggcactg gtcacccagt tccgcacgct gctggtacgc acctacgccg gcgcgctggc
    12961 cgcgttcaac aaccagacca tcgaattcaa accattcaac atgccacagg gcagtaccga
    13021 cgtgaccgtg gaaacccagg tcaagcagcc tggcgctcag ccgatcccca tcaattacag
    13081 catggagcag accggcgatg gctggaaggt gtatgacgtc agcatcgacg gtgtcagcct
    13141 ggtgaccaac taccgcggtt cgttcggtac agagatccgc cgggagggta tcgacggtct
    13201 gctgcgcacg ctgaccgcac gcaaccagca ggacacggct gccactgctg cggtcaaata
    13261 ggcaatgatg agcgcactcg ccatgccatc ggccattcac gtactgccgg aacggattga
    13321 cctgaccacg gtgcaggaag tgctggccgc tgcgtcgaac gcgctggaaa acggcagccg
    13381 ggtattcgat ttcagcgccg tgaaccagct cgattcgact gcgctggcgc tggtgctggc
    13441 gtgcgcacgc atggcacgcg aacgccatgt ggaaattcgt tgcatcaatg tgtcggacaa
    13501 tctgttgagt cttgcacggc tgtacggcgt ggacactttt cttcctgtcg aggcctcgtg
    13561 accggcactc ccgcacttga agtcagtcat ctgcacaagt gtttcggcgc actgcaggcg
    13621 ttgcgcgatg tcagcctgcg cgtcgaacag ggcgaatttt ttgccctgct cggaccaaac
    13681 ggggcgggca agaccacgct gatcagcgtg cttgccggcc tggcgcgcgc ggacagcggc
    13741 gaggcgcgca ttctcggtca ttgcgtggta cgggactaca aggcggcgcg acgtcaactg
    13801 ggcgtggtgc cgcaggagct ggcctacgac ccgttcctga ccgtgcgcga gaccctgcgc
    13861 ttccagtcgg gatattacgg cctgcgccac aatgacgact ggattgacga agtgctgcag
    13921 cgtctcggcc tggatgacaa ggccgatacc aacatgcgcg cgctgtcggg cggcatgaaa
    13981 cgccgcgtgc tggtcgcgca ggcgctggtg caccggccac cggtcatcat cctcgacgag
    14041 ccaactgccg gcgtcgacgt ggagctgcgc cagtcgctct ggcagttcat ccgtcagctc
    14101 aatgcttcgg gtcacaccat cattctcacc acgcattatc tcgaagaagc cgaagcgctg
    14161 tgccagcggg tggccatgct caagcagggt cgcatcgttg ccctggacac taccgccagc
    14221 ctgctcagtt ctgcaggggg actgcgtttc gaagttacgc tggacagcga ccggttgccc
    14281 gaagcgctcg ccggacgtgc agtcgaactg ggcgaacacc gctactgctt cagtctgacc
    14341 tctgccgccg agctggaaat gatccttgcc ggtttgcgta ccgccggcat cggcacgcgc
    14401 gatctggcac tcaatccccc cgacctggag gagatcttcg tgcgcatcat ggagcatgca
    14461 tgagcggctt tcgtaccctg ctgtacaagg aattgctgcg tttctggcgc gtctggttcc
    14521 agaccattct tgctccgatc atcacggcct tgctctacct gctgatcttt tcgcacgcgc
    14581 tggcccatca tgtcgaagtc tacgccggca tcggttacgt cgaattcctc gtgccaggac
    14641 tggtaatgat gtccctgctg cagaatgcct tcgccaacag ttcgtcgagc ctgatccagt
    14701 cgaagatcac cggcaacatc gtgttcgtat tgctgccgcc gatttcctgg ctcgaatttt
    14761 tcctcgccta cctgctgggt tcgattttcc gtggtctgac cgtgggcgcc ggggtatggc
    14821 ttggcaccat gctgttcgtg gcgccacata tcgaacaccc tctcatcagc ctggtgttcg
    14881 ccggcctggg cggcggcatg ctcggtgcgc tcggtatcat tgccggcatc tggtcggaaa
    14941 aattcgatca gctggctgcg ttccagaatt tcatcatcat gccgctgact tttctttctg
    15001 gcgtgtttta ttccatccac agcctgccag cgttctggtt caatgtatcg cgcttcaacc
    15061 ccgtgttcta catgatcgat ggcttccgtt tcggtttctt cggcgtcagc gatgtggcgc
    15121 cacacaccag tctgctggtc gttctgacca gttttgtggc attatccgca ctttgtcttg
    15181 ttttattgaa acgcggttac aagctgcgtt attgacggtg gacacatgag catggttgaa
    15241 ccagacagca tccgcaacag tattcgcgat ggactgtcct gtgagcaggt acgcgtgacc
    15301 ggagatggtc atcatttcga ggcatgggtg atcagtgccg actttgaagg caaatcgcgc
    15361 gtgcagcggc accagatggt gtatgcaacg ctgggcgacc ggatgcgcga agaaatccac
    15421 gcactgtcga tccatgcact gacgccgcaa caatggatcg aggcgcaagc ctgatggaca
    15481 aactggccat acgcggcggc aaacggttgc agggagaaat ccgtatttcc ggcgccaaga
    15541 acgcggccct gccgatcctg tgcgccagcc tgctcaccgg cgagccgctg tatctggaca
    15601 acttgcccag gctcaatgac atcagcacca cgctgacact gctgggccac atcggcgtac
    15661 agatcgcgct tgacgaacgt ggtggtgtcg aattgcatgg cgcggcgctg actcggctcg
    15721 aggcaccata cagcctggtc aagaccatgc gcgcctcgat cctggtgctg ggtcccatgc
    15781 tggcgcgctg cggcgaagcg cgcgtctcgc tgcctggcgg ttgcgccatc ggcagccgcc
    15841 cggtggacct gcatatcaag gggctgcagg ccatgggcgc ccagatcgac atcgatcacg
    15901 gctacatcct tgcgcgcgcg gggcggttgc gtggcgcacg cattttcatg gatacggtca
    15961 cggtcactgg cacggaaaac ctgatgatgg cggccacgct cgcctccggc accaccattc
    16021 tggaaaatgc cgcccgcgaa ccggaagttg tcgatctggc tgattgcctg atcgccatgg
    16081 gtgccgacat tcgcggcgca ggcagcgata ccatcaccat tcagggagtg gaacgtctgc
    16141 acgccgcgca ttaccgcatc atgcccgacc ggatcgaaag cggcacctac ctggttgccg
    16201 cagccatgac cggcggggcg atcacgctgc gcgacagttg cccgcaggcg ctggacgcgg
    16261 tgatcgaaaa actgcgcgaa gcgggtgcgg tgattcactg cgaccacgat tcgatcgatc
    16321 tgactgcccc cgcacggctg cgtgcggtca acctgcgcac ggcaccgtat ccggcatttc
    16381 ccaccgacat gcaagctcag ttcatggcac ttaacgccac ggccgaaggc gtcgggcggg
    16441 tgaccgaaac cattttcgag aatcgtttca tgcatgtgca ggaaatgcgt cgcctggggg
    16501 cacagatcga aatcgaaggc aataccgcgc tggtacgcgg tgtcgagcac ctgcaaggag
    16561 cgaccgtcat ggcgaccgac ctgcgcgctt ccgcctcgct ggtgctggct gcgctggtcg
    16621 ctgaaggtga aaccgtgatc gaacgcattt atcatatcga tcgtggctac gaatgcatcg
    16681 aggaaaaact caaccagctt ggcgccagtg tgcgtcgcat ccattgactg tcgatatgga
    16741 agacgccggt tcggtacatg tggcagatcg agcagtgtcc gcgccatgaa acccgacgcg
    16801 cttgcagtca atagcacagc gctttttcag gagtattgcc catgatcacc atcgcactgt
    16861 ccaaaggccg catctgggaa gaaactgccc cgctattgca actggcgggc atcgaagctg
    16921 ccgagaaccc ggagaaatcg cgccggctca tcatcggcac caatcgtccc gacgtgcgtc
    16981 tgctcatcct gcgcgcgacc gatgtaccga cctatgtgca gcatggcgca gccgacatgg
    17041 gtatcgctgg tcgtgatgtg ctgatcgagc acggcagcga aaacctgtac cagcctgtcg
    17101 acctgcagat cgcccgctgc cgcatgatgg tggctgctca tccagcgttc gattaccaga
    17161 atgccgtgcg ccagggcgca cgcatccgcg tggccaccaa atacttgcgt atcgctcgcg
    17221 atttcttcac cgagcgcggc gtacacgtcg atctgatcaa gctgtacggt tccatggaac
    17281 tggcgccact ggtcaaactg gccgatgtca tcgtggacct ggtgagcagc ggcaatacgt
    17341 tgaaggccaa cggactggtc gccgtcgatg atgtcatgga aatcagttcg ctgctcatcg
    17401 tcaaccaggc atcgctcaag ctcaaacgcg aaaagatcca gccaatgctc gacagctttg
    17461 cacaagccgc cgtggctacc gcctgaacgg acatatgccc gcacacctgc cacaggatgc
    17521 gagcatccgt cgcattctca tcatcaaatg gagcgctctg ggggacatcg ccatgtccac
    17581 ctgcatcatg gaagacgttg tccgcgcgtt tccacaggcg gaaatcgatc tcaataccat
    17641 gccgccctgg gaccgcctgt ttaccggcga tccgcgcttt tcccgcgttc tcgccatcaa
    17701 cgtgcgcgac cgcaagcacc cgtggcgtgc ggtctggcaa tggttgcgtg aagtgcggcg
    17761 tggccggtat gacctgattg tcgacctgca gtccaatgac cgttcccggt tgctgctcag
    17821 cctgttgcaa tggagcggcc gccgcacccg ccacatcatc ggtaaccgat cgggacgggt
    17881 gtggcctttc agtggcaggc agccggatca accactgaac ccggtcgacc gcatgcgcga
    17941 cagtcttcgc gcggcgggga ttccggcgcg gacagcccgt ccggtattcc acatcgccgc
    18001 acacaaccgc gagcgcgcgc aggcactgca gcaccgccat gggttgcaac cgggaaatta
    18061 tgcgctgttt taccccggct gccaggcagc aggctacctc aaacgctggg gagaagcacc
    18121 gtatgccgag ctggcgcgtc gcttgctgga cagcggtcgt gtacagcgtg tggtgctgat
    18181 cggcagtgca gacgaaatgg aagattgccg caacattgcc gcgctggcgg gtgagggtgt
    18241 ggtcaacctg tgcggcgaaa ccgaagtgct tgatatcgtt ccgctggcgc tcggtgccgc
    18301 gctacaggtg ggcaatgata ccggcaccgc gcatctcgcc gccgcatcca gcgcaccgat
    18361 ggtggtcgtg tgcggtccga ccgacgcctt tcgcgtgcgg cctcaaggcg acaacgtcat
    18421 cgccatccag gcacagttgc cctgcatcaa ctgttattgc cgccatccct gtgaccatca
    18481 cagttgcatg aaggccatcc acccggaata cgtattgcag gcgctcgacg gcaaacaggg
    18541 caacgtggtg atgctgcctg cgtcctgatg ctgttaccgg ctgcagatct ctctgtcaga
    18601 ccgttgaaaa aggtagcgag gacgcccgga tgcaaggcgc gagaaacgca acgacgtgac
    18661 atatcaaata gataggcatg ggagtgagta tcgcagcaac gccgcagacg ggtgccgcag
    18721 tactttttca acggccagct ataccggcct gtcggattcg tataccggtg gcggtacggg
    18781 cgcacccggt ggcagcaggc tcatgccgta acgctggcgc tggtgatatt cgtacagttt
    18841 ggcatacttg agaaacacgt agaacgcacc gcacaccgaa gtaatgaatc cgccccagcc
    18901 attgaggaat gcgcgcttga aaaaataggc acgtatgaaa tacaccggcg gataaaacac
    18961 gaaaatccac gggctggcgg aacgaccctt ggccaccttg tcacgcacca atccggtcga
    19021 ataggcgttg agcttggcca ctttgacgtc gatatcggtt tcgccgtaat ggtagaatgg
    19081 cgacctgagt gacaggatgc gtccatccac cttgggtgcg gcatgcaccg gcatgtcgct
    19141 cacatggccg cggtccttgc ggaacaggcg caggaacttg ttcaggcgga tgtaatcgtt
    19201 gttcatctgc cagaacatct gttccttgcg cgggatgaca tagccgtcag cctgcgggcc
    19261 gtcctgcagc agtttgcgga tttcctgctg gcattccggc gatagggctt cgtccgcatc
    19321 gagcagcagc acccacaggt ggctggtctg ttccagtgcc agctgtttct gtttgccgta
    19381 accgagaaac gggtggtgat ggatcgtgca cccatactga cgggcgatgt ccagtgtggc
    19441 atcgctgctg ccagaatcga gcagcacgat ttcgtcagcc cattttacgc tttcgagaca
    19501 actggccagg gtgcgtgcat tgttgaacgt ggtgacaaag acggacaagg gttccatgtg
    19561 tccaggcggg aaaaatgaca atgcttcgaa ctataacaca gcgccaggcg ggcaattgaa
    19621 gatactgcac ctgatcggct cgagcggtac cggcggggct gaacgccacc tgatcgacct
    19681 gtgccggcag caggcggcca tggggctcga tgtcgccgtg gccttgccga cgcgcggtac
    19741 gctgggccgg acgctgcaat cgctcgggat tgactgcttt ttcatccgcc cgggtgggcg
    19801 ctggaacctg ctggcgctgt ggtcgctgcg tcaggccatc cgccgtgccg cgcccgacct
    19861 gatccatgcg cacatggtca aatccgcgat catggcacaa cgtgccggta gcggcgtgcc
    19921 ctgcgttgcc accgcacata acctggtgcg ccgctgcggc ccgtttcgcg gctgccgacg
    19981 cgtgatctgc gtctcggccc aggtgcgcga cacattgacg gcctgcggct atccgcctga
    20041 ccacagcctg gtggtaccca atgccatcga tttcagcctg ctgtcgaacc gtccgcgcgc
    20101 tgcagaacgc gagcgcatgg gctgggcgca acaaccggtt gtgctgtgtg tggcgcgact
    20161 ggtgccggcc aaaggtcacg tcttcctgct gcacgccatg gcgcacgtct ggcagcagtt
    20221 tcccgctgcc cggctggtgc tggccggcga agggccggaa cgtgccgcgc tggaacaact
    20281 ggcggcgcgg ctgcaggcca cggcacaggt cgattttctc ggcaaccgcg acgatgtgcc
    20341 gcaactgctg ggtgcggcgg atgtttatgt acagccgagc atcaaggaag gtttcggcat
    20401 cgccctgctc gaagccatgg ccagtgcctt gccgtgcatt gccacgccta ccggagcgat
    20461 gggggagatg atcgaaaccg gtcacaccgg tctgctggtc gcccccggcg atgctgacga
    20521 actggcgcag gcaatcctga aactgctggc tgacgcccca ttgcgccagc aactggcggc
    20581 ccacgccagc gagacggtgc gcagccgtta cagccaggcg cgtcagggcg acgcaacgct
    20641 cgcagtctat cgtcaggcca tgcaggccgc ttgcgagtcc cgatgacaca gaagggacac
    20701 aacagctgat ctcctcacat ggatatgctg atcaaatcac atgccatgag tctcgggttc
    20761 catcgaggtc accatccgtt cagggagacc tgaaaaccaa caggaatctg caactttaca
    20821 cgaagttgaa gtcattcctg gtgggtacag tgcgagctgg aagttatacc gcgcagttca
    20881 tcttttcgag atcagcgatc aaaccaggtc ctgcaggatt ccatcccagc ttcttgcgtg
    20941 tgatggcgca tgaggcaggc atgtcgagcc cggcaaacat cgcaagccaa ccgaaatggg
    21001 ctggagcttc ctcagcggta atggatttga ctggcaattt caggctacga ccaagaactt
    21061 cggcaacatc acgcatcgaa acgccttctt ctccgaccgc atggtagcgc gcaccttttt
    21121 cagctctttc aagcgctagc agatacagac gagcgacatc agtgacatgt cctgccggcc
    21181 aacgattcaa cccatcccca atgtaaaaag agacaccttc ttcacgcacc atcgggatga
    21241 ggtacgaaat cagcccaaat ttttcgatgt tgtgaacttg cggcaagcgc attactgaaa
    21301 catttacacc tcgctccagg atagcgttgg cagcgaattc cgaagcagcg cggggattcg
    21361 ggtgattgcg attccagtga tcttcggtgg cacagtcgcc tggtgcctta ttccccatgc
    21421 ctgttccaga agtaattatg atcggtttat cggagccttc cagaacagct cccattgctt
    21481 cgatagcgcg cttgtcgttt tcgcaattct ctgcgaatct ggaaaaatcg tggttgaacc
    21541 caagatgaat cacagcgtcc gccgctgctg aacctgttcg caacgactcc aaatcctcga
    21601 tattgccacg atgcacttct gcaccagcag caatcagtgc cttggctgct tcgtcggaac
    21661 gggcgagccc caataccaga tggttagcat caagaagttc tcggacgaca tggctgccga
    21721 taaatccagt tgcgccggta acaaatacac gcataggtaa ttcctccaat gattgaatgc
    21781 aggtgaatgc attatcggca cgtggtatac actattaaag tagtgatatt atacgggtat
    21841 aaatattaac cggatcagat catgccttcc aaaactgaca atccactagg gagttacctg
    21901 aaagatcgtc gcgaaaaact tgacccggcg gcgcttggtt ttccagtttc acgccgccgc
    21961 actccaggat tgcgtcgtga agaggtagcg cagcgcgcca acgtcagtac aacctggtat
    22021 acatggcttg aacaaggacg aggcggcgca ccatcgcctg ctgtaacaga acgaatagca
    22081 cgcgcactca tgttgacgga agcagagcgg gagcatttat atctgttagc attggggcgt
    22141 ccgcccgaac cacgctatcg gtccatggaa ggcattatac cgcgtttaca acgccttttg
    22201 gacgtcctgg aaatcagccc ggctttcatg cgaacagcca catgggatgt cgtcgcctgg
    22261 aatgctgcgg ccacagtggt atttggcgat tacagcaaac ttgcaccttc ccagcgaaat
    22321 atactgagac tctttttttc caacgcacaa gctcgatcag ttcaaacaga ttgggaaagt
    22381 gtcgcgcgtt ttgtcgtcgc agctttcagg gcagaggtaa tgcgtgccgg agctgcaaat
    22441 gatgttaagt cgttaatcga tgagctctcc gagctaagcc cggagttcag gttgatatgg
    22501 caaagcaatg acgtgggtgg gtctggaggg ggtaaaaaac agttgcgcca tcctgtggcc
    22561 gggctacttg aattggaata ttcgtcattc gctgttgacg gtcgcccgga tcttaccctt
    22621 gtgatttaca atccggccac tcctgaagat gtagtg
//
LOCUS       DAIANL010000061        15466 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1291022, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000061 DAIANL010000000
VERSION     DAIANL010000061.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 15466)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15466)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15466
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291022"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3353 a         4195 c         4888 g         3030 t
ORIGIN      
        1 gagatggtga tgcctggcga caacgtgtcg gtgaacgttg cgctgattgc gccggtggcg
       61 atggaagaag ggctgcgctt tgcgatccgc gaaggtggcc gtaccgttgg cgccggcgtg
      121 gtggccaagg tgatcgagta acaaagaaaa tacgcagggc agtggctgca ggcgctgccc
      181 gatgatgacg tattaggcca gtagctcaat tggcagagta tcggtctcca aaaccgaggg
      241 ttgggggttc gaggccctcc tggcctgcca aacttaccgg agtgtgccag ccccgccgat
      301 gaacggcggg gcgcacggat ttcagatgac cgatcagatc aagttggtgg cggccatagt
      361 gctggtcgtt gcgggaattg tcggttacta cgtgtttgcc gacagcgcca ctgcgctgcg
      421 tgtgctgttt gtgctggccg gtggggctgc cggggtcggt gtggcctggc ctactgccct
      481 ggggcagcgg ttctacgggt tttcacagga atcgatcaag gaagcgcgta aggtggtgtg
      541 gcctacgcgc aaggaaacca tgcaaactac aggcgtggtg ttcgcgttcg tggtggtgat
      601 ggcgattctg ctgtgggttg tggatgccag cctgctgtgg gtggtgaggt ggttgatggg
      661 gcggggtgat tcatgagcaa gcgctggtat gtggttcacg cctactcggg tttcgaaaaa
      721 acggtccagc gtacgttgct ggaacgcatc gaacgttacg gcatgcagga caagttcggc
      781 cagattctcg tgcctgtcga ggaagtggtc gagatgaagg gcggtcagaa gtcgatttcc
      841 gaacgcaagt tctttccggg ttatgtgctc gtggaaatgg aaatggacga cgaaacctgg
      901 cacctgatca aggacacgcc aaaagtgact ggctttgttg gtggctctcc caccaaaccc
      961 acgccgatca gtccgaagga agtcgatgcc atcctgcacc agatccagga gggtgtggag
     1021 aagccacggc ccaagatcct gttcgaggtg ggcgagtcgg tgcgtgtgat cgacgggcct
     1081 ttcaacgatt tcaacggtac ggtcgaagaa gtcaattacg acaagagcaa gttgcgcgtg
     1141 tcggtgctta tttttggtcg ttccacgcca gtggaactgg atttcggtca ggtcgaaaag
     1201 acctgaccgc ggtcggatcg tctcatgacg gtccggatca tgaacgggga gggtctggcg
     1261 cgcaagcggc cggatccgtc attacccatt tgaggagcgc acagtggcaa agaaaatcgt
     1321 tggctacgta aagctgcagg tcccggccgg caaggccaat cccagccccc ccatcggccc
     1381 ggcgctgggt cagcgcggtc tgaatatcat ggaattctgc aaggcgttca atgcccagac
     1441 tcagggcatg gagcccggtc tgccgattcc ggtggtcatc accgcctatg ctgacaagag
     1501 tttcacattt gttctcaaga caccgcctgc gaccgtgctg atcaaaaagg cggcgggcgt
     1561 gcagaagggc agccagcgtc cgcataccga taaggttggc aaactgacgc gcgcccaggc
     1621 ggaagaaatc gccaaggcca agatgccgga tctcactgca gccgatatgg atgctgcggt
     1681 gaaaaccatt gccggcagcg cccgcagcat gggcatcgaa gtggagggcg tgtaaatgtc
     1741 tgcaatttcc aaacgtcaac aggcaatccg cagcaagatc gaccgcaacc gtacttatcc
     1801 ggtcaatgac gcgctcgcgc tgatcaagga caccgctaac gccaagtttg acgaatcggt
     1861 ggatgtggcg gtcaatcttg gcattgatgc acgcaaatcc gaccagttgg tgcgtggctc
     1921 ggttgtgctg ccgcgcggta ctggcaaaac agtgcgtgtg gccgtgttcg cccagggcga
     1981 agcggcgaag gcggcgcgtg acgctggtgc tgacatcgtc ggtttcgaag accttgcagc
     2041 cgagatcaag ggtggacgca tggatttcga cgtggtgatc gccagccctg acgccatgcg
     2101 ggtggtcggt cagctgggtc aggtacttgg cccgcgcggt ctgatgccta accccaaggt
     2161 gggtaccgta acccctgacg ttgcgggtgc ggtgcgtaac gcacgtgccg ggcaggtgca
     2221 ataccgtacc gacaagggtg gcatcatcca gtgcagcatc ggccgtgcat cgtttgaagt
     2281 ggatgcattg cgcgagaacc tgtcggcgct gatcgatgcg ctcaacaagg ctcgtccggc
     2341 ttcagccaag ggcgtgtaca tgaaaaagat ttccgtctcc agcaccatgg gtgttggcgt
     2401 gcgggtggat ggcggcagca ttgctgcata agcaggctcg tcattcgtgc aggactttgt
     2461 gggccgccgg gtaaccttcc ggcgggttgt caaagaccgt tggggttggg ctggcagttg
     2521 ctgcccgact taatgggaag tttcgcagtc tgaaacatcc gcccaacgca gacggcgtac
     2581 ccagaaccag gaaaaatgca gcagacatgc ttgattgctc ctgatgaaag gtcgtcgatt
     2641 ctcgagctgc accgtccgtc aggccggtgc cgtccggagt tcatggaagg agatgggacc
     2701 ttgagtctta atctggttga aaagcaagcg gttgtaacgg agatgagcga acggctgctt
     2761 accgcccagg cgattgttgt cgcggagtat cgcggaatga ccgtggtcgg tatgaccgga
     2821 ctgcgcgcca aggctcgcgc cagtggcgtg tacctgcggg tgctgaagaa cacgctggtt
     2881 cgccgtgcgg ttgccggtac ccattttgcc gggctgtccg accatctggt cggtccgctg
     2941 gtatatggcg tatcggaaga tccggttgcg gtggccaagg tgttgtccga atttgccaaa
     3001 accaatgaca agctgatcat caaggctggt gcgatgccga atttcgtgat gtccgccaag
     3061 gatgtcgcaa atctggcaaa catgccaagt cgcgaagaac tgctggcgaa actcatgggt
     3121 accatgcagg cgccggtggg acagtttgtc cgtacgctca acgaagttcc gacccggttc
     3181 gtgcgcggac tggcagccat tcgcgacaag caggctgcct gaacccgctc tggcgcggtt
     3241 cgcttacgca tgatccggta gtacggaacg ttgcaacgaa ttcattcatt catcaggaga
     3301 tacaaacatg gctttgtcca aagctgaaat tctggatgcc atcgctggca tgactgttct
     3361 cgaactgtcc gaactgatca aggacatgga agaaaaattt ggcgtgtcgg ctgccgcaac
     3421 cgccgtggct gttgctgctg ccccggctgc cgctgctgct gctgccgttg aagagaagac
     3481 cgaattcgac gtcattctga ccaaggctgg cgacaacaag gtcaacacca tcaaggtggt
     3541 gcgtaccatt actggcctgg gcctcaagga agccaaggat ctggtcgatg gcgcgccgaa
     3601 gccggtcaag gaaggtgtgc ccaaggctga agccgagtcg atcgtcaagc agcttaccga
     3661 agccggtgct gcctgcgaaa tcaagtaagg ctgttcctgc gctgcccggc ctgccgggca
     3721 gcgcaggatg cagaagacaa cgacatggcg tttgccggtc gttgtcttct gtcttctgcg
     3781 caagttcccc gagggagatc tcatgagcta ctcattcacc gagaaaaaac gcatccgtaa
     3841 aagttttgcc aagcgtgcaa atattctgca ggtgcctttt ttgttggcga cgcaaatcga
     3901 ctcctaccgt gccttccttc aggaagatac ggctcccgag gcacgcgcga acgaaggcct
     3961 tcaggccgcg ttcacctcga ttttccccat ccagagccat tcgggcaacg cccgactcga
     4021 cttcgtcagc tatgtgctcg gcgaaccggt attcgatatg gtggagtgcc agcaacgcgg
     4081 cctgacattc gcatcgccgc tgcgtgccaa ggtgcgactg acgctgatgg acaaggaagc
     4141 tgcccgccca tcggtaaaag aagtcaagga acaggaagtg tacatgggcg aaatgccgct
     4201 catgacgccg accggttcgt tcatcatcaa tggtactgaa cgggtgatcg tgtcgcagtt
     4261 gcaccgatcc ccgggcgtat ttttcgagca cgatcgcggc aagacgcaca gttcgggcaa
     4321 gctgctgttc tccgcgcgca tcatccccta ccgtggttcg tggctggact tcgagttcga
     4381 tgccaaggac tacctgtatt tccgtattga ccgtcgccgc aagatgccgg tgaccacgct
     4441 gctgaaatcg ctgggttatg acaacgagcg cattctggcc gaatttttta ccaacgacac
     4501 cttccatttc ggaaaggatg tggtgcgttt cgagttgatg cctgatcgtc tgcgcggtga
     4561 aatcgctcgc ttcgacatcg ttgacaaggc cggtaacgtt atcgtgtccc gcgacaagcg
     4621 tattacggta aaacacatac gcatgatgca ggaagccggt ctgagtcaga tcgccgttcc
     4681 gaatgaattc cttcttggtc gtattctggc caggaatgtg gtcgatactg ccacgggcga
     4741 aatcatcgcc ctggccaacg acgagttgac cgaatcgctg ctggaaaagc tggttgctgc
     4801 cagtgtggcg cagatcgaaa ccctgtacgt caatgacctt gaccaggggg catatatttc
     4861 tgcaacgctg cgtaccgacg atacgcccga ccagtattcg gcacgcgtcg cgatttatcg
     4921 catgatgcgt ccgggcgaac cgcctacgga agaagctgtc gaggccctgt atggcgggct
     4981 gttctttagc gaagaccgct atgacctgtc ggcagtcggt cgcatgaagt tcaaccgccg
     5041 ggtcggtcgc gacgcgctgg aaggtccggg cgtactgtcc accgaggaca tcgttgccgt
     5101 gatccgcatt cttatcgaac tgcgtaacgg ccgtggtgag atcgatgata tcgatcacct
     5161 gggcaaccga cgcgtgcgca gtgttggcga attggcggaa aaccagttcc gcgctggtct
     5221 ggtgcgcgtg gaacgcgcgg tgaaggaacg cctgtcgcag gccgaatccg aaaacctgat
     5281 gccgcatgat ctgatcaacg ccaagcctgt gtcggccgcg atcaaggaat tcttcggttc
     5341 cagccagttg tcccagttca tggaccagac caaccctctg tccgaaatca cccacaagcg
     5401 ccgcgtgtcg gcactgggtc cgggcggtct gacacgtgaa cgcgccggtt tcgaggtgcg
     5461 tgacgtgcat ccgacgcact acggccgcgt gtgcccgatc gagacgccgg aaggcccgaa
     5521 catcggtctg atcaattcgc ttgcgctgta tgcgcgcacc aacaagtatg gttttctcga
     5581 gacgccgtac cgcaaggtgg aaaatggtca cgttaccgag cacatcgagt acctttcggc
     5641 gattgaggaa agcaagtacg tcattgccca ggccaacgcc gaactcgacc tcaacggccg
     5701 ttttaccagt gagatcgttt cctgccgttt caagaacgaa ttcgaactgg ctacgccgga
     5761 gcgcatccag tatatcgaca tcgcgccgtc gcagatcgtc tcggtggcgg catcgctgat
     5821 cccgttcctc gaacacgacg acgccaaccg ggcgttgatg ggttccaaca tgcagcgcca
     5881 ggccgtgccc tgtctgcgtg ccgaaaagtc tctggtgggc accggcatcg aacgtactgc
     5941 tgccgtggac tcgggtacga ccgtgcaggc acgacgcggc ggcgtggtcg actatgtcga
     6001 cgcggggcgt atcgtcgtgc gtgtcaatga cgacgagacc aaggccggcg aagtcggggt
     6061 ggatatctac acgctgatca aatacacacg ctccaaccag aacaccaata tcaaccagcg
     6121 tccgctggtc aaagtgggtg accgcctgca acgcgctgac gtgattgccg atggcgcatc
     6181 aaccgatatg ggcgaactgg cgctgggaca gaatctgctg gtggcgttca tgccgtggaa
     6241 cggcttcaac ttcgaagact cgatcctgat ttcggaacgt gtggtcgccg aagaccggta
     6301 cacctcgatt catatcgagg aactgaccgt cgtggcgcgc gacaccaagc ttggaccgga
     6361 agaaatcact cgcgacatct ccaacctgtc cgagcgcatg ctgagccggc ttgacgaatc
     6421 gggtatctgc tacattggcg ccgaggtcga ggccggagac gtgctggtgg gcaaagtgac
     6481 gcccaagggt gaaacccagc tcacaccgga agaaaagctg ctgcgcgcca tttttggcga
     6541 gaaggcgtcc gatgtgaagg atacttcgct gcgtgtgccg tccggcatgg ccggaacggt
     6601 catcaacgtg caggtgttca cccgcgaggg catcgagcgt gacgctcgcg ccaagcgcat
     6661 catcgaagac cagctggccg cgtacaagaa ggacatggcc gaccgcatgc gcatcgttga
     6721 aaacgacact tttgcccgct tgcagaaact ggtgctgggt aaagtggcgc gtggcggtcc
     6781 tggcaagctg gcgcgtgaca gcgcgattac gcaggactac ctcgatgcgc acaaccggta
     6841 cgactggttc gatatccgcc tggccgatga cgacgcaagc cgtcagatgg aactgctcaa
     6901 ggacagcctg gaccagaagc gcgttgaatt cgatcagatg ttcgaagaga agaaaaagaa
     6961 gctgaccgcg ggcgacgagc tgcagcccgg ggtgcagaag atggtcaagg tctacgtggc
     7021 ggtcaagcgt cgcttgcagc cgggtgacaa gatggccggg cgtcacggta acaagggtgt
     7081 ggtttccaag atcgtgccga ttgaagacat gccgttcatg gcggatggca cgccgatgga
     7141 catcgtcctt aacccgctgg gcgtgccatc gcgcatgaac gtcggacaga ttctcgaaac
     7201 ccatctgggc tgggctgcca agggtctcgg caagaaaatc gacgacatgc tgcaggcgca
     7261 acgcaaggtg ggcgaattgc gcgatttcat cgatcagatc tataacaccg ccggcaagcc
     7321 ggaacaggtg gcgcagatgg acgatgtgga agtgctggag ctggccagca atcttcgcaa
     7381 gggcgtaccg tttgccaccc ccgtgtttga tggcgcctcc gaagaggaaa tcaaggccat
     7441 gctcaagctg gctgacctgc ctgaaagcgg tcaggtcacg ctgttcgatg ggcgcacggg
     7501 cgaagcgttc gaccgcaagg tcaccgtggg ctacatgcat gtgctcaagc tgcatcatct
     7561 ggtcgatgac aagatgcacg cgcgttcgac cggtccgtac agtctggtga cccagcaacc
     7621 gttgggcggc aaggcgcagt tcggtggcca gcgttttggc gagatggaag tctgggcgct
     7681 tgaagcgtat ggagcctctt acatcctgca agagatgctg acggtcaaat cggacgacgt
     7741 caatggtcgt accaaagtgt atgaaaacat cgtcaagggc gaacacaaga tcgatgcggg
     7801 tatgccggaa tccttcaatg tgctggtgaa ggaaatccgt tcgctggcca tcgacatcga
     7861 tctggagcgc tactgaaccc gggttggaca gtattcacgg ttcgtagacg cctctttgga
     7921 gaaagtacat gaaagctttg ctggatcttt tcaaacaagt cacccaggaa gaagagttcg
     7981 atgccatcaa gatcggcctc gcttcgcccg agaaaatccg cagctggtcc tacggcgaag
     8041 taaaaaagcc cgagaccatc aactatcgca ctttcaagcc tgagcgtgat ggcctgttct
     8101 gtgccaagat ttttggtccg gtcaaggatt acgagtgcct gtgcggcaag tacaagcgcc
     8161 tcaagcaccg tggcgtgatc tgcgagaagt gcggtgtcga ggtgacgttg tccaaggtac
     8221 gccgcgaacg tatgggccat atcgaactgg ccagcccggt tgcgcacatc tggtttctga
     8281 agtcgctgcc atcgcgtctg ggcatggtgc tcgacatgac gctgcgtgat atcgaacgtg
     8341 tgttgtattt cgaagcctat gtggtgaccg acccgggcat gacgccgctg acccgcgcgc
     8401 aattgttgac cgaagatgac tatctcgaca aggtcgaaga gtttggcgac gagtttcgcg
     8461 ccagcatggg tgccgagggt atccgcgagt tgctcaaggg catcgacatc aacggcgaac
     8521 ttgattccat tcgttcggag ctggccagga ccggttccga caccaagatc aaaaagctgg
     8581 ccaagcggct caaagtgatc gaggcattcc agaaatccgg gatcaagcct gaatggatgg
     8641 tgctggaagt gctgccggtg ctgccgcccg aactgcgtcc actggtgcca ctcgatggcg
     8701 gtcgttttgc cacttcggat ctcaatgacc tgtaccgccg tgtcatcaac cgcaacaacc
     8761 ggctcaagcg actgctggaa ctgaaggcgc cagaaatcat cgtgcgcaat gaaaagcgca
     8821 tgttgcagga agcggtggat tcgttgctcg acaatggtcg gcgcggcaag gcgatgaccg
     8881 gtgcgaacaa gcgtccgctc aagtcgctgg ccgacatgat caagggcaag ggcggccgct
     8941 tccgtcagaa cctgctcggc aagcgcgtcg actattccgg ccgctcggtc attgtggtcg
     9001 gcccgcagtt gcggttgcat cagtgcggcc tgccgaagaa gatggcgctg gaactgttca
     9061 agccattcat tttccacaag ctggaagtca tgggtcttgc gaccaccatc aaggccgcca
     9121 agcgcatggt tgagaccgag gaacccgtgg tgtgggatat cctcgaagac gtcatccgcg
     9181 agcatccggt gctgctcaac cgtgccccga cgctgcaccg tctgggtatc caggcattcg
     9241 aaccgacgct gatcgaaggc aaggcaatcc agttgcatcc gctggtgtgc gcggcgttca
     9301 acgccgactt cgacggtgac cagatggccg tgcacgtgcc gctgtcgctg gaagcccaga
     9361 tggaagcccg cacgctgatg ctggcatcga acaacgtgtt gtcgccggcc aatggcgagc
     9421 cgattatcgt gccgtcgcag gatatcgtgc ttgggctgta ttacatgaca cgcgaacgcg
     9481 tcaatgcgcg tggtgaaggc atgatgtttg ccgacgtgag tgaagtgtcg cgtgcttacg
     9541 aatcgcgtca ggtcgaactg aatgcgaagg tgcaggtgcg catccgtgaa gcgtggtacg
     9601 acgaggccgg tgagcgccag gaaaaacgta cccgctatga aaccacggtg ggccgcgcgt
     9661 tgctgtcaga aattctcccg gtcggactgc cgttcagcaa tatcaacaag attctgaaga
     9721 aaaaggaaat ctcgcgtctg atcaatacca gcttccgtcg ctgtggcctg cgtgagacgg
     9781 tgctgctggc agaccgcctg atgtataccg gctttaccta tgcaacgcgc ggcggcatgt
     9841 cgatctgtgt tgatgacatg ctggtgccca aacagaagca cgacatcatc agcgcctccg
     9901 aaaaggaagt gcaggagatc gagtcgcagt acatttccgg tctggtgacc cagggcgaac
     9961 gctacaacaa ggtggtggac atctggggtc gcgcgggcga ccaggttgcc aaggcgatga
    10021 tgaaccagct tggttccgag gccgtggttg accgcgaagg caagcagacc acgcaggaat
    10081 cgtttaacgc catttacatg atggccgatt cgggtgctcg cggttctgca gcgcagattc
    10141 gtcagctggc cggcatgcgc ggtctgatgg ccaagcctga cggctcgatc atcgaaacgc
    10201 ccattaccgc gaatttccgc gaagggctga atgtgctgca gtacttcatc tcgacgcacg
    10261 gtgcgcggaa aggcctggcc gacaccgcac tcaagaccgc aaactccggc tacctgaccc
    10321 gccgcctggt cgacgtgacc caggatctgg tggtgatcga ggatgactgc ggcaccagcg
    10381 aaggcgtggt gctcaaggcg ctggtcgagg gtggtgaagt gattgaagcg ctgcgcgatc
    10441 gcgtgctggg ccgtgtgacc gcgctggacg tgatgcatcc ggaatccggt gacgtggtga
    10501 tcgccgctgg tgtcatgctc gacgaagcga tggccgaaca gctggaagcg ctcggcattg
    10561 acgaagtcaa ggtgcgtacc gcgctgacct gcgagacgcg ttacggcctg tgcgccaagt
    10621 gctatggccg cgatctcgga cgtggtcacc tggtcaacgt gggcgaagcc gtcggcgtga
    10681 ttgccgccca gtcgatcggt gagccgggaa cgcagctgac catgcgcacg ttccacatcg
    10741 gtggtgcggc atcgcggtct gcggccgcaa gccagctcga atccaagtcg gcgggtacgg
    10801 tacagtacag tccggccatg cgttacgtga ccagtgccaa gggcgagctg atcgtgatcg
    10861 cgcgcagcgg cgagctgctg atcatgaatg acagcgggcg tgagcgcgag cgtcacaagg
    10921 tgccttatgg tgcaacgctg atggtcaagg acggcgaaaa gatcaaggca ggccaggtca
    10981 tggcgatgtg ggatccgcac acccggccga tcattaccga atatgccgga cgcgtgcgct
    11041 tcgagaatat cgaagaaggt gtgaccgtcg ccaaacagat cgacgaagtt accgggctgt
    11101 ccacgctggt ggtcatcgat ccgaaacgtc gcgtcggtgt tgccagcaag gggttgcgcc
    11161 cgcaggtaaa gctgcttgac agcgatggcg tcgaagtcag ggtggcagga acggatacgc
    11221 ccgtcaacat cacattccag atcggctcgc tcatcaccgt gaaggatggc caggaagtgg
    11281 gggttggcga ggttctggca cgtattccgc aagaatcgtc gaagacccgc gacatcaccg
    11341 gcggtttgcc gcgcgtggcc gaactgttcg aggcgcgctc gcccaaggac gctggcgtgc
    11401 tggctccggt gaccggcacc gtgtcgttcg gcaaggacac caagggtaag cagcgcctgg
    11461 tgattaccga tctcgatggc gtaggccacg agtacctgat ccccaaggaa aagcacgtca
    11521 tggcccacga tggccaggtg gtgaacaagg gtgagatgat cgtcgacggc ccggtggatc
    11581 cgcacgacat tctcgcgctc aagggggtcg aggcactggc gcgctacatc acagacgaag
    11641 tgcaggacgt gtatcggctg cagggtgtga aaatcaacga caagcacatt gaagtcatcg
    11701 tgcgccagat gctgcgtcgc gtgagcatca ccgagccggg ggatacccgt ttcatccagg
    11761 gcgaacaggt ggagcgtgcc gaaatgctga tcgaaaacga acgcgttcag gccgaaggca
    11821 agacgccagc cgcgttcgat tacatcctgc tcggtatcac caaggcctcg ctgtcgaccg
    11881 attcgttcat ctcggccgcg tcgttccagg aaaccacgcg cgtgctgacc gaagcggcaa
    11941 tcatgggtaa acgtgacgag ttgcgcggcc tgaaggaaaa cgtcatcgtc ggtcgcctca
    12001 ttccggccgg taccggtctg gcttaccact cccagcgccg tcgtcagaag ctggaaggcg
    12061 atacgttaag cgaacttgat gtgttcgaac ccgtcgagac tgtgggtgaa gagcctgctg
    12121 cttgacaaaa tgggggccgc ggatgtaata tccgtggtct ttttgcccgg taacgaattt
    12181 ccgggcggat tcggtttatt gatttgattt attgaagctg ggacaagtat gccaaccatc
    12241 aatcagctgg tgcgcaagcc gcgcgccgcg aaacgtgtaa agagcaaggt gccggcgctg
    12301 gaagcctgcc cgcagaagcg tggcgtgtgc acccgtgtgt ataccactac gccgaagaag
    12361 ccgaactcgg cattgcgtaa ggtggcccgt gtgcgcctga ccaacggttt cgaggtcagc
    12421 agttacattg gcggcgaagg gcacaacctg caggaacact cggttgtgct gattcgtggt
    12481 ggtcgtgtaa aggacttgcc tggcgtgcgc taccataccg tgcgtggcag cctggatacc
    12541 gccggcgtga aagatcgcaa gcaaagtcgt tccaaatacg gcgcaaagcg ccccaagaaa
    12601 gcttgatcgg gcgcctgtgt gcttcgccgc gtcgtacttg cggccgcatg cccggaaaag
    12661 gttgagagga taatatgcca agacgcagag aagtacccaa acgcgatgta ttgccggacc
    12721 cgaagtttgg tagccaggat gtcgcaaagt tcgtgaatgt gctgatgacg agcggcaaga
    12781 agtccgttgc agagcgcatc atctatggcg cctttgatca gatcagccag aagaccggca
    12841 aggatccggt cgaggtgttc acgcaggcgc tgagcaacgt gcgcccgctg gtcgaggtga
    12901 agagccgccg tgtcggcggc gccaactacc aggtgccggt ggaagtgcgt cctgcgcgcc
    12961 ggtctgcgct ggccatgcgc tggctcaagg agtcggcccg caagcgcggc gagaaatcca
    13021 tgggcattcg tctcgcaggt gaactgctgg atgcggctga agggcgcggc ggtgcgatga
    13081 agaagcgtga tgaggtgcac cggatggccg aagccaacaa ggccttctcg cacttccgct
    13141 tctgatccgg gcgcaacggt cggaagacaa tgtacaggca ggctgttggt tacaacagcc
    13201 tgtttgattt gatcgaatcg taacacatgc aggctgccgt gcagacgtcg gtctaaggaa
    13261 tggaaaaagt ggcacgcaaa actcccattg aacgctaccg caacataggc atcagcgcgc
    13321 acatcgacgc tggcaagacc accaccaccg aacgcatcct gtattacacg ggcgtgtcgc
    13381 acaagattgg tgaagtgcac gatggtgccg ccaccatgga ctggatggaa caggagcagg
    13441 agcgcggaat caccattacg tcagcggcga cgacctgcta ctggcgtggc atggaaaaca
    13501 actatcccga gcaccacatc aatatcattg atacgccggg acacgttgac tttaccatcg
    13561 aagtcgaacg ctccatgcgt gtgctcgacg gtgcctgcat ggtgtactgc gctgtgggtg
    13621 gcgtgcagcc acaatcggaa accgtgtggc gtcaggccaa caagtacggc gtgccgcggt
    13681 tggcgttcgt caataagatg gaccgtactg gtgcgaattt cttcaaggtc tacgaccaga
    13741 tgaagtcgcg cctcaaggcc aacgtcgtgc ccattcaggt gcccatcggt gcagaggaaa
    13801 aattcgaagg cgtcgtcgat ctggtaaaga tgaaggcgat ctactgggac gaatcttcgc
    13861 agggcatgcg tttcgacgag cgccccattc ccgccgactt gcaggccgaa tgcgacacgt
    13921 ggcgcgaaaa gatggttgaa gccgcggccg aagccagcga agacctgatg aacaagtacc
    13981 tcgaagaagg cgagctcagc accgaagaga tcaaggctgg cttgcgcacc cgtaccattg
    14041 cgggcgagat catgcccatg ctgtgcggat ccgcgttcaa gaacaagggt gtgcaggcga
    14101 tgctcgatgc ggtgatcgat tacctgccgt caccgaagga tatccctgcg gtcaagggcg
    14161 agctcgagag tggtgaggtg gccgaacggc atgcggttga cgacgagccc ttttctgcgc
    14221 tggcaatcaa gatcatgacc gacccgttcg tcgggcagat gatttttttc cgcgtctact
    14281 ccggcgtgct taattcgggc gacaccatct acaaccccat caagggcaag aaagagcgta
    14341 tcggccgcat tctgcagatg catgcgaacc agcgttccga aatcaaggaa gtgcgtgccg
    14401 gtgatatcgc cgctgccgtt ggtctcaagg aggccacgac cggagatacg ctttctgatc
    14461 tgcagaaagt gatcgtgctc gagcgcatgg tgttccccga gcccgtgatc cacgtcgctg
    14521 tcgaacccaa aaccaaggcc gaccaggaaa aaatgggcat ggcgctcaat cgtctggcgc
    14581 aggaggatcc ttcgttccgc gtgcgtacgg acgaagaatc cggtcagacc atcatttcgg
    14641 gcatgggcga gttgcacctt gaaatcatcg tcgaccgcat gaagcgtgag ttcggtgtcg
    14701 aggccaatgt cggtgcgccg caggtggcct atcgcgaagc gattcgcaag gcggtcgagg
    14761 tcgaaggcaa attcgtcaag cagtccggcg gcaagggcca gtatggccac gtctggctca
    14821 agcttgaacc gaacgaggcc ggtaagggct atgaattcgt cgatgccatc aagggtggta
    14881 ccgtgccgcg cgaattcatt ccggcagtgg acaagggtct gacggaaacg ctgccaaatg
    14941 gcgtgctggc cggcttcccg gtggtcgatg tcaaggttac gctgttcgac ggctcctacc
    15001 atgacgtgga ttcgaacgaa aacgctttca agatggctgc gtccattgcg ttcaaggatg
    15061 gcatgcgcaa ggccagtccc gtgctgctcg aacccatgat ggcggttgaa gtcgagacgc
    15121 cggaggatta catgggtgac gtgatgggtg acctttcctc ccgtcgcggc atgattcaag
    15181 gcatggacga caatgccagc ggcaaggtca tcaaggctga agtcccgctg gcggaaatgt
    15241 tcggctattc cacggtgctg cgttcgatga gtcaggggcg cgccacctac agcatggaat
    15301 tcaagcatta tgccgaggcg ccgaaaaacg tcgctgaagc gatcatcaac aagaagtaac
    15361 tgattggcaa ggacaggaca tcatggcaaa aggcaagttt gaacggacca agccccatgt
    15421 gaacgtaggg acgattggac acgttgacca cggcaagacg acgctg
//
LOCUS       DAIANL010000062        27435 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_71561, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000062 DAIANL010000000
VERSION     DAIANL010000062.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 27435)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27435)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27435
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71561"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5511 a         9211 c         7782 g         4931 t
ORIGIN      
        1 gaccgcttcc accgcacgca gcaccccctt gccctggtag cggttggcat cgccatcgcg
       61 caactcgatc gcctcgcggc tgccggtgga cgcaccggac ggcaccgccg cccgccccag
      121 ttcgcccgac tcgagcagca cgtcggcttc cacagtcgga tttccgcgcg aatcgagaat
      181 ttcccgcgca ataacatcaa cgatcacact catttcattc tccagatccg tcacacccga
      241 ctcagagggt gtgctctata aaaccatgct gtttgaccag cgcgtcaatt tctttcaggg
      301 tgtacagcaa cgctttcatg cgatcgagcg gccaggcatt cggtccgtcg gacagggcgc
      361 aggcagggtc gggatgcgtt tccgcaaaca gccccgacac acccgcggcc acggccgccc
      421 gcgccagcac cggcacaaac tcgcgctgac cgccggatgc gtgcccttgc ccacccggca
      481 actgcaccga atgcgtggca tcgaacacca ccggacaacc ggtgtcacgc attgccatca
      541 gtgatcgcat gtcagacact agattgttgt aaccgaacga caccccgcgc tcgcacacga
      601 ggatgttgtc ctgcccgcca ttggctgcct tggccttgtc gaccacgttc ttcatgtccc
      661 agggcgccag gaactggcct tttttgatgt tgaccggttt gccggcgcag gccaccgcct
      721 ggatgaaatc cgtctgacgg cacagaaagg ccggagtctg cagcacgtcg accacttcgg
      781 ccacctctgc gatctcgccg atgtcatgca cgtcagtgag taccggtacg ccgatttcag
      841 cacgcacatc cgccaggatc tgcagcccgg catcgcggcc gggaccgcga aatgacgcgc
      901 ccgaactgcg attggcctta tcgtacgatg atttgtaaat aaaggggact tcgagttcgg
      961 cgcacagctc gcgtagcgca ccagcggtat ccatggccag ctgacgcgat tcgatcacgc
     1021 acggcccggc aatcagaaac agcggacgat gcaatccggc ttcaaatccg cacaatttca
     1081 ttgcttttcc tcccgcacac gcagggcggc ctgaacatag gcgcgaaaca acggatgccc
     1141 gtcgcgtggc gtcgacgtga attccggatg gaactggcat ccgacgaacc atggatgccc
     1201 gggcagttcc accatctcgc acagcccatc ggtcgccgaa cgaccactca cgcgcagtcc
     1261 ggcctgctcc aggcgcggca acagcgcgcc attgacctcg taacgatggc gatgacgctc
     1321 gacgatgcgg tcagcgccgt agacgctgcg cgccagcgtg cctggcagca ggttgcagct
     1381 ctgcccgccc agtcgcatgg taccgcccat gtcggactga tggctgcgca tctcgatctg
     1441 gccggcagcg tctttccatt cagagatcag cgccaccacc ggccagtcgg tggcagtgtc
     1501 gaactcggtg ctgtgcgcac ctgtcatgtc agcacagtgg cgcgcgaatt cgatgacggc
     1561 aagctgcatg cccagacaga tgcccaggta aggcacgccg tgctcgcgcg catggcggat
     1621 ggcgagcatc ttgccctcga cgccgcgttt gccgaaacct cccggcacaa ggatggcatc
     1681 catgccggca agcgcaccca cgccgtcctg ctccagctgt tcggaatcga tgtagtgcac
     1741 attgacctgg gtgcgggtgt ggatcccggc gtggatcaac gcctcggaca acgatttgta
     1801 cgattcggtc agcccgacgt acttgcccac catggcgatg tccacagtgt gctgcggatg
     1861 ttcgagcgct tcgaccagac cattccacat cgacaggtcg gctggcggcg catcgatacc
     1921 gagccggcgg cagacaatgt cgtccagccc ctgttcacgc aacatgccag gaatcatgta
     1981 gatgctgtcg acatcgtgca cggaaatcac ggcgttggct tcgagattgg tgaacagcgc
     2041 gatcttgcgc cgctcgtctt cgggcagcgg gcggtcgccg cggcagagca ccacatccgg
     2101 ctggataccg atttcacgca actccttcac cgaatgctgc gtaggcttgg tcttgatctc
     2161 gccggccttg ccgaggtagg gcaccagtgt cagatggatg taacaggtat tattgcgacc
     2221 ttcgtgcaag cccatctgac ggatggcttc aagaaatggc aacgattcga tgtcgcccac
     2281 ggtaccgccg atttccacga tggccacttc agcgcccgcc gcaccttcct ggatatgcag
     2341 gcgaatctcg tcggtgatgt gcgggatcac ctgcaccgta ccgcccagat agtcaccacg
     2401 ccgctccttg cggatgaccg actcataaat ctggccggta gtaaaattgt tgcgccgaga
     2461 catgcgagta tggatgaacc gctcgtagtg acccagatcg aggtcggatt cggcgccgtc
     2521 ctcggtgacg aaaacttctc catgctggaa tgggctcatg gtgcccggat caacattgat
     2581 gtacggatca agcttgagca gggtgacccg catgccgcgg gattccagaa tggcagccag
     2641 cgacgcggcg gcaatcccct tccccagaga ggacaccaca ccgccggtta cgaagatata
     2701 acgtgtcata tcgattcaca gtgcaggtaa tgctgaattc tagcccaatc cgctgttcgc
     2761 cacaattgcg cacgcctgtc ccggcccgcc tgccgctgaa atgcggcgat tttccgctgt
     2821 catggcccac attgaccatt ttgccatgat atttattaca acagcgcttt gcctgcgcac
     2881 ccttctggcg tagaatccct cactggcggg tctccccgca ttgcaaggcg gtcaatctgg
     2941 tcaggtccgg aaggaagcag ccacagccgt cggctgcaag tgccggggtt caggctcgcc
     3001 acccgaacat accgccacgg gcgggcggtg caaaccgcgg acaggccggc ccgggcccct
     3061 gacaggcata gtatggacaa cacctctacc cactttggct accagaccgt ggccgaatcc
     3121 gaaaaggccg cccgtgtcgc cggcgtgttc cattccgtcg cgtcgcgcta cgacctgatg
     3181 aacgacctga tgtccgccgg cctgcaccgc ctgtggaaac aattcaccgt tgccacttcg
     3241 ggtgtccgcc ctggcgaacg cgtactcgac gttgccggcg gcaccgccga cctggcacgc
     3301 cagtggatca aaaaaaccgg ccgcaacggc gaagtctggc tgaccgacat caacagctcc
     3361 atgctcacgg ttggccgcga ccgcctgctc gacgaaggca aagccattcc cgtcgcccag
     3421 tgcgatgccg aacacctgcc attccccgat aattattttg actgcgtgag cgtggcgttc
     3481 ggtctgcgca acatgacgca caaggaccag gcgctggccg aaatgtttcg cgtgctgcga
     3541 cccggcggcc gcctgctggt gctcgagttc tcgcgcatct ggaaaccgct cgaacccgtc
     3601 tatgacgcct actcgttccg gctgctgccg ctgatggggc aactggtggc caaggatgcc
     3661 gagagctacc gctatctggc cgaatccatc cgcatgcacc cggaccagcc gaccctcaag
     3721 cgcatgatgg aagatgccgg cttcctccgc gtcacctgga acaacctcac cggcggcgtg
     3781 gtcgcgctgc acaagggtta caaattctga tccagtcatg ctcgccaaca tttccgaccg
     3841 cctgctgtgc tccgccctca atcatctgct tgcacaggca ccctgggcac gacgcaaggc
     3901 catgctgctg gccgggcaat ccatccgcct gaacctgttc ccgctgcaat ggacgctggc
     3961 gttcaacgcc gaaggctgga tcgaaccatc gaccaccgaa ccgctggcca cgctgcgcat
     4021 cacaccgttt ggcgcaatcc gcatcgcatt tgacgaagcg caggctcaac atgcggtgga
     4081 gattctgggg gacgcccagc tcgccgcccg tttcggcatg atcctgcgca cgctgacctg
     4141 ggacgcgcag gctgaccttg cacggttgct gggtgactcg gccgcccatc tgatcacgca
     4201 taccatcaac gagtggatgc gacgtaccaa cgacaccctg ctcgaacagg cgcgcgcctg
     4261 gatggaatac gccaccgaag aaactccgct gctcgcggga cgggcgcgca tcgccgcctt
     4321 tgtcgccgac attgatgcac tgcgtgaccg caccgcacgc ctcgaacagc gcgtcagtct
     4381 gctcgcgcaa cgcctggcca cggagcgcaa ctgctgatgc gtgtgttccg cctggtcacc
     4441 atcctgtggg ttgccgcacg ttatggtctg gacgaatttt ttcttggcaa cgagcgcaca
     4501 cgcggcttgc gcatattggt gcgcttcgcc ctgttctggc gcgacctgtc cgcaccgcgt
     4561 gcggtacggc tgcgtctggc actggaacgg ctgggaccga ttttcgtcaa gttcggccag
     4621 atgctgtcga cccgtcgcga tctcatgccg cccgacatcg ccgacgagct ggcccgattg
     4681 caagaccggg tgccaccgtt cccggccgcg caggcccgct ccgcgctcgc tgccatttac
     4741 ggcaagccgg tcgagcaggt gttcgcccgt ttcgatgccg aaccggtcgc cagcgcatcg
     4801 atggcgcagg tacacttcgc cgagctgttc gatggccggc cggccgcagt caagatcctg
     4861 cgccccggca ttacccgcat catcaaccac gatctcgatc tgctggaatc cgccgcgctg
     4921 ctgatggaaa agctgttcat cgacggccgc cgtctcaagc cgcgcgaagt ggtcagcgag
     4981 ttttccaaaa cactgcacga cgaactcgac ctgatgcgtg aggccgccaa ctgctcgcag
     5041 ctgcggcgca atttcgaaca ctccgaattg ctcatcgttc ccgaagtgta ctgggaatac
     5101 tgcgaatcgg gcgtgatggt catggaacgc atgcagggca tccccatcag ccgcaaagac
     5161 cagctggtgg ccatgggcat cgacacccag cggctggcgc gcgatggcgt ggaaatattt
     5221 ttcacgcagg ttttccgtga tgcctttttc catgccgaca tgcacccggg gaatattctg
     5281 gtcaacgcgc aggggcatta catcgcgctc gatttcggca tcatgggcac gctcaccgaa
     5341 caggacaaaa actatctggc gcaaaatttt ctcgcctttt tccgccgcga ctaccgcgcc
     5401 gttgcccagg cgcacgtcga tgcaggctgg acgcccgccg atacacgcgt agacgaattc
     5461 gaggccgcca tccgcacggt gtgcgaaccg attttcgacc ggccgctcag ggaaatctcc
     5521 tttggcaagg tgctgctgcg cctgttccag acctcacggc gtttcaacat ggaaatccag
     5581 ccgcaactgg tgctgctgca aaaaaccctc ctcaacatcg aaggcctcgg acgcgaactc
     5641 gaccccgaac tggacctgtg gaaaaccgga aaaccctttc tcgaacgctg gatgagcgaa
     5701 caatccggct ggcgtggctg gatgcgtaat ctgcaacagg aagcgcccta ctgggcagca
     5761 gccctgccgc agttgccgcg tcttgcgcac gccttgctca aacacgcgca taacgaacag
     5821 aacaacacgc aactccagtt gctggtgaaa cggcagaaac gcagcctgaa acggttgcgg
     5881 gtggccaccg gagtacttgc tgccgtcctg ctgctcaccc tcggctggct gatccgctcc
     5941 cacatctgac cgagagaccc gcacaccatg ctcatctggt ttgtcgtcat ctacctgttc
     6001 atcgccatcg gcattggcct gtatgccgcc accaaggtgc acaacgcccg cgattacgtg
     6061 gtggccggac gcgcattgcc gctgtacatg gtcacggcca cggtattcgc cacctggttc
     6121 ggttccgaaa ccgtgctcgg cacctcggcc acctttctgc aggatgggtt gcacggcatc
     6181 gttgccgacc cgttcggcgc cagcctgtgc ctgattttcg tcggcctgtt ttttgcgcgc
     6241 aagctctacc gcatgaatct gctgaccatc ggcgactatt accggctgcg ctacagccgc
     6301 acggtagaga cgctgaccgg catggccatc gtcatttcct acctgggttg ggtatcggcg
     6361 cagatcaccg cgatcggtct ggtgttttca ctggtaagcc aggggcagat cacgccacac
     6421 aacggcatgc tgatgggctc ggccatcgtg ctgatctata cgctgttcgg cggaatgtgg
     6481 tcaatcgcgc tgcacgattt cctgcaaatg aacgtcatcc tgatcggcat gctatatatt
     6541 gcctgggtca tcagcggcga tgcgggcggc gtgatgcacg tcatccacca ggccgaccag
     6601 caggggaaat tcaatttctg gcccaaaccg gacctgcctg acatcctggc attcttcggc
     6661 gcctgggtca ccatgatgct cggatcgatc ccgcaacagg atgtgttcca gcgcatcatg
     6721 tcggccaaaa acgagcatgt tgcagtgcat ggctcggtgc tcggcggttc gctgtacttc
     6781 ctgttcgcct tcatcccgat ttttctcggc tattccgcga cgctgatcga tccgggcatg
     6841 gtcaagcagt tgctcggcga acatccgcaa catatcctgc ccaacctgat cctgcaccac
     6901 acgccgatct ttgcccagat catgttcttc ggtgcgctgc tgtcgtcgat cctcagcact
     6961 gccagcgcca cgttgctcgc tccgtccatc accttcaccg aaaacgtcat caagccgctg
     7021 atcggccaat ggggcgagcg tcatctgctg ctggtgatgc gtggcgtggt ggtcggtttt
     7081 gccggcctgg tcgcgttcta tgcgctcagc tccaacagca gcattttcaa tatggtggaa
     7141 aacgcctaca aaatcacact ggtgagcgca ttcgtgccac tggcagccgg actgtactgg
     7201 aagcgcgcca ccaatcaggg ggcgctgctg gccatcatcg ccggtgccag cacatggatg
     7261 tggctggaaa ccaccaatcc tgacggcctg tggccacccc aactgttcgg cctgctgatg
     7321 agcatcaccg gcatggtggt cggctcgctg ctgcccagcg tggtgcccgg cgagcgcgag
     7381 cgcgaagacc ggcacgggtg aggcgccacg gctgcacatg gacgattgca ctggtgctgg
     7441 ccgggttctg gctgactggc gcgccggtgt tcgccaattt cgtgctcgcc cggctggaaa
     7501 ccgctccgat cgtgactacc accgccctga atcaggggca ggccattgtc atcctcggcg
     7561 gcggcacgcg gcgcggtgct cctgaatacg gcgacgacac tgtcagcgac tacacgctcg
     7621 aacgattacg ctatggcgcg cggctggcgc gcgccaccgg cctgccggtg gccgtgagcg
     7681 gcggcagccc gcacggcgga caggccgaag cccggacgat gcgcagcgcc ctcagggacg
     7741 atttcggcat caccgtgcgc tggagcgaag accgctcgcg caacacgtgg cagaatgcag
     7801 tactgctgcg cgcgatgctt cccccggata ttcgccgcgt ggtgttggtc actcacgcct
     7861 ggcacatgcc gcgggcatgc tacgcattcg aacacgccgg cttcagcccg atcgccgcgc
     7921 ccaccggcta cgtcgcccgc cgtccgctgc gatgggaaga tttcatgccc gggccgcgcg
     7981 gctggagcgc cacctggctc accgtgcatg aaatgctcgg catgatctgg taccgcctgt
     8041 caggcccggc acacctggca agcgttgacc ttcttctgca tcagtcccgg tgaaagatcg
     8101 gcgctttcct gacggcaaca cagaatgttc aacagctgct gcggctttgc tacaatccgg
     8161 ggtattgtcc ctcctggagc agtcacatgc aggttcgcgt caaatgggta ggcaacatga
     8221 cttttctcgg caagtccggc agcggacacg ccgtggtgat ggacggcccc gtagaaggca
     8281 gcgatgcgcc tctgctcggg ccacggccga tggaaatgct gctgttgggc gcggggggct
     8341 gcacctccta cgacgtggtc agcatccttc gcaaggcacg tgcacaggtg cgcgactgtg
     8401 aagtggaagt ccgtgccgag cgcgccgaca gcgaccccaa ggtattcaca cacatccacc
     8461 tgcatttcgt ggtcaccgga cacgcgctca aagccgaact ggttcagcgc gccatccatc
     8521 tttcagccag caagtactgt tcagccacca tcatgctggg caagacggcc gaaatcacgc
     8581 atgacttcga aatcgtcgag gatcagttcg attcgcagcg taccgagtaa cttgcttcgc
     8641 cacgctcgac cgcaagcagg cggtcgatgt tggggtgctt gccagggtgg gcacgggcat
     8701 cctcgacatg ttcggcaaac agttccagcc cttcctgtgc agccttcacg ccgatcccgc
     8761 cccagcgcag cgccagatgg taatacacgc ggaatgaacc gctgctgccc ggctggttga
     8821 gaatttcccc acacggcttg ccctcggcat cgaacagccg catacgccgc cactgatcgg
     8881 tggcttccag tttcgtcaga ttatcggcaa acattacgaa ctcgcttcct cacagatgta
     8941 ccatcaaatc cagtaagcca ccgaagtcat ggtgcgcgcc atccacttca tcgccccgcg
     9001 caccggcaat ggcagttctg cggcaccaaa cgacactgcg gtatcggcat gcttcgcctc
     9061 gtcgattttc atctgttcga cgacggcacg gctgcgcacg tcttcttcag gcagcttgcg
     9121 caagtgcgaa tcgaggtggg caccgacctg gcgctcggtt tcggccagaa accccagatt
     9181 ccagcggtca ccaagcaccc cggcagtcac cccgatggca agcgagccgg cataccagag
     9241 cggattgagc aggctttttc gaccgcccaa cgcctgcatg cgcgcctcgc accaggccag
     9301 atgctcgatt tcttcctgcg cggccttggc cagcgcatgt ttcgctacat cattacgcgc
     9361 ggtcagcgcc tgtccctgat acagcgcctg ggcgcacacc tcacccacat gattgacgcg
     9421 catcaaggct gcggcagcac gctgctgcgc ctcgctcatt tccgtctcgg gcaacgcagc
     9481 acccggcagg gcacgctggc tggtggccgg agcaaacacc gtgcgcaagc cgttttccat
     9541 cgccatgatc cagtcatcga gattcatcct gctccatcct tttgcaatcg tggcacaatg
     9601 cacgcatgaa gcccggtatt ataagcctga catgcctgct gctgttttcc gtttcggccg
     9661 cctgcgccac cgggctgccg ccctccgtac aggccgacct ggaccaggcg ggcatccctg
     9721 acagccatgt cggcatcgtg gtatggccgc tcgaccgtga tcagccgcaa cttgcgttga
     9781 atgcagagca acccttccca cccgcgtcca cgatgaagct gctgaccagc cttgcggcac
     9841 tcgatctgct cggacccgcc tggcactggc aaactgaagt ctggcatgac ggcacgctgc
     9901 acgatggcac gctggacggc aacctgtacc tgcgcggcaa tggcgatccg ggatttaccg
     9961 acgaacgact gtggctgctg ctgcatgaac tgcggctgga tggcctgcaa acgatccacg
    10021 gcgatttaat catagaccag aaccgttttc aatcggcgct tgccgccccg ttcgacgacc
    10081 acccgctgcg cgcctacaac ggtatgccgg cagcaaccct gcttgatttc ggcagcagca
    10141 cagtgcgcat cgacagccgg gacggacaca gcctgctgga tgtcgacccg ttgccggctg
    10201 gcgctcgtct tgacaaccgc atccatgtcg accacgcagc atgtggcgac tggcgcgaac
    10261 gcatccatac cggctggcag ccagaatcag gcgtactccg ctttgacggc gactggtcgg
    10321 accaatgccc gcaaagtcag tttgccatca cgctgcacaa ccccagcgcc ctgctcggcc
    10381 cggccttcat caccggctgg caacagcagg gcggcacgat cgacggcacc tggcgcgagg
    10441 gcaccgtcgc tcccgacgcg cagctgctgg cgtcattccc gtcgctgccc ctggcaaccc
    10501 tgctgggcga catgaacaaa ttcagcaata acgtcatggc acgcaatctg ttcctgtcgc
    10561 ttgccaccga tactccggcc actcccgctt ccgccgcaac ggcgttgcaa cattggctgc
    10621 actcgcatgg gctatccatg cctgaactgg tgatcgacaa tggttcggga ctgtcacgca
    10681 gcgctcgcat ctccccactg gacatggcac gactgctacg ccaggcgacc cgctcggcgc
    10741 tgtggccgga atttgccgcc agcctgccca ttgctgccgt cgatggcacc atgcggcacc
    10801 ggctgcgcga cacgccggtc gccggacacg ctcatgtcaa aaccggcacc ctgaccggcg
    10861 tgaaaaccat tgccggatat gttaccctcg cggacgggcg gcgtgtcatc gtggtgtttt
    10921 tcatcgatga tgcgcacgca gccaacggtg ccgccgcgca agaggcgttg ctgagcgcca
    10981 tctattccgg cctgcccgaa taaaccgccc cggcgcacgt cctcttgccc atggcaacgg
    11041 atcgatccgg aaaaccaggg acatcccaca ggatagcgcc atgtccacca cccagcagga
    11101 acccagttcc atcctgattc cgttcacgga aacccgctgc cgcccgggtg atgtgctgca
    11161 gctacagcta ccgggtgccg aacaacgtta tccgacgaaa ctgatcggtt acgtcaacga
    11221 aaaaaccatt ctggttactg caccgggcaa cggcagccag ttgctgatgc tgcgcgaaaa
    11281 ccagagcctc accgtgcgct cgttctccgg taccagcgcc tacgcctacc acagccaggt
    11341 catcaggaca tgcaattcac ccttacccta tctgcacctg tcctggccaa agtccgtgca
    11401 caaggtaccc atcaggggat cgacacgggt ggactacaac ctgtcgggta cggtgctcaa
    11461 cctgaatcag gacgccagcc gcaaccaata cccggtgcag atcgtcgatc tcagcaccag
    11521 cggcgccgcc ttcgttgccg ttcgaccggt cggcaagaag aacgacgtgc tgcgactgag
    11581 attcacagca aagatccatg ccatcgaagt cagtccggat ctgaacagcg tgatccgttc
    11641 ggtgcaaccc ggcgaagacg gtacatcgat ccgttatggc atccagtttc tcgatctcag
    11701 cctgctggac acgctgaccc tgcagggtat ggtgtgtcag caggtgtttt cggatcgctg
    11761 aaacgcgcgc tcaggtcttg gcaccggtgc catacaggta gtgtgagaac tgggcgtaat
    11821 cgctgctcac cgaacggaac agcttgtcga ggctcaggat cgcctgctcg gccaggttga
    11881 tggcgatata cgggtgttcg accttcatgc gcttgtacat ggcgcgcgac aggcgcagca
    11941 cctgagtgtc gtcagcgcgc gtcaccatgc gcaccgagcg tgcgcgcttg tcgaagaacg
    12001 acatcatgcc gagcatttcg cccacgccgg aacgcacgat ttcatattcg gtctggctgt
    12061 cagggatcac cagcgcgatt tcgcccttga cgacgaaata cagttcgtcg cccacatcgc
    12121 caatgtcgga aatcacatca ccgttgtcga aatgcagtac ggccagataa tgcaacaggg
    12181 tgttgacctc tgccaccgtc aatgattcgc agaggtattg ctggctcagg aactgagcca
    12241 gttcggcgtg ttgggccaat tgagtcatca ccatcgtttt ttgctcctta tgagtacttc
    12301 atgcctgatt cattcgcttg aggcggctcg ctgcatgagg cgcaggccac ccagcatgcc
    12361 ctcgatccgg ccatgcagcg ctgacagcag ctgcccggag cttgctgcgc cgggctcgaa
    12421 cggatagtgc cgatgctgca tatgcgtcgg ccggcaaggc atatccgcca gagccgccgc
    12481 atccagtgtc accagcagat caccccattc gagatcggcc ttgctgcaac aagcaagatt
    12541 accgccagcc tgcgccagac ggcgcgcgcg cgcttccatc cagcccgcgc catgccggtt
    12601 ggcccagtcc gccgcttcgg cggcaagtcc gtccgtgcgg ccggtgagaa acaccacccg
    12661 tgccggccgc agatagccgg aacccttctt ttccccgccc tgtgtgtcca tcgcgctatt
    12721 gtaccgcggg ttcgcgcaac gcggcgtcgt gacggcattt tcatccgggc ggctggctgc
    12781 cgtgttcagg ttgaggtaac atttcggcac tcaccattcc gtccgcccat acactgtgca
    12841 tcatcagctc attccacttc tgcgcattct gtccgacgga aaattccatg ccggctcaga
    12901 actggcgcaa caactggaca tctctcgcgc cacggtatgc aacgccctca aatcggccag
    12961 cgacgttggc atccgcctct acaaggtgca aggacgcggc taccagatgc ccgtgatccc
    13021 ggactggctg gatgcaacac aaatccgcgc cgggctgcgc ggcgaggcca gcgggttcac
    13081 catcgaaatc gtcgattcga tcgaatccac caacacggcg ctgctgcgcg cccccgaacc
    13141 tgccacccac cgccactgcc tgctggccga ataccagcat gccggacgtg gacggcgcgg
    13201 ccgcagctgg ctggcctcac tcggcggcag cgtgacctgc tcggtacgct ggcgcttcca
    13261 gcagggcatc ggtgcgcttg cgggactgag cctcgccgtc ggtgtggcgc tactgcgctg
    13321 cctcgacgca ctgggcgccc gcgacctcaa actcaaatgg cccaacgatc tgttatggca
    13381 ggatcgcaaa ctggccggca tcctgatcga agtccagggc gaagccaacg gtccctcggt
    13441 agccatcatc ggcattggca tcaatctgcg cctgccagac agcctgcgca acaccatcga
    13501 gcagccggta accgacctca ccgaggccat cggccacccg gtcgcacgca acgcattgct
    13561 gtccgacctg ctcaacgccc tcgacacggt catgcgcgaa ttcgaagcca gcggtctgag
    13621 ccagctgcgg cacgaatggt ctgccgcaca cgcccacgcc ggccagacgc tgcaggtcac
    13681 gcttgccaac ggccatctca tcaccggcgc ggcgtgcgga ctcaccccct ggggagccct
    13741 gttgctgcaa gacgacaacg gcgccgaaat cgccgtacac agcggtgacg tgcaactggc
    13801 gcgcaaggca tcctgaacca tgttgctggc catcgatatc ggcaacaccc gcatcaaatg
    13861 gggtttgcac gacggcactg gctggctcga acgcgacgcg gccacgacac tggacgcctt
    13921 gccggcccac ctcgacgcac tggccgatca ggtcatcctg agcaatgtcg gcgcaccgtc
    13981 cctcagcggc atgctgacgc gccgcttcgg tcggctgcac gaaatccacg cccagccaca
    14041 acaatgcggc gtgcgcaacg gctaccgcaa tccggccagc ctgggcagcg accgctgggc
    14101 agcgttgatc ggcgcccatg cgctcggcgc acgcacggca ctggtcatca atgccggtac
    14161 tgcgctgacc gtcgacgccc tgcaccttgg tgattttctc ggtggtacga tcagccctgg
    14221 ctaccggctg ctgacccgtt cgctgaccga ggcaaccggc ctgcatggca cgcatgacgc
    14281 catctggtcc ggtttccccg acaacaccgc atcggcgctg ctgaccggtt gcattgcaag
    14341 cctgaccggt ccgatcacgc atatgcacga tacacttgcg cagcaatgcg ccacacaacc
    14401 cgagctatgg gtcgccggcg gcgatgccgc actgatcgca acgcacctga cctgccgtac
    14461 cgaaccgcac ctggtgctcg aaggactgct gcacatcgcc acggaggtat tcgcatgaaa
    14521 ttcctcgcca tcctgctcgt gctgctcaat ctcgcactat tctcctggtt tgaatgggat
    14581 caaccggcga agcagcgaga acccgagccg gccgagttcc atcccgaacg cgtgcaggtg
    14641 atccatctgc catcaacggc gcaccagcct ggcgctgtcg caatgacaac cgggcaatcg
    14701 tccgcgccgt caccgtctgg caacgcggct gccggctcac ccgccgccgc agcttcgccg
    14761 gcaccggcca ctcccgctgc gacacctgcg ccggcaccag ctgcgccagc cgcaccggca
    14821 acgcccacgc cccatgcatc gacggatacg cctgccacag ttccggcaac ggttcccgct
    14881 ccggcgcctg cagccagcag cacaactccc cccggccccc agaccacaaa cgctcctgca
    14941 tcagccgccg gcaccgcaat ctgcctgcaa tggggcccga taccagccga ccgtaccgcc
    15001 gaggcgcaaa cccgactcaa ccagctgctg cttggcgaac ggctcatcag ccacgacagc
    15061 gacaacgcca gcggccccta ctggatctac tacccgccac tgcgtgacaa gcaggctgcc
    15121 gatgcccgcg tcgcagccct cgaagcccgg ggcattcgcg acgtcagcgt aatccgcaac
    15181 ggtccgtggc agaatgccct gtcgcttggc gtctatggca agctcgacat ggcgacccaa
    15241 cgtctgaccg ccctgcgcaa gctgggcgta catgcacaac ttgaagcgca cggcaacgct
    15301 gcgcgcagct tcaccctgct gcatctcgac agcaccgaac aaactgcagc ccgggccatc
    15361 gaccgtgaca tgaagcttgc gcccatgcgc cctgtcgact gcccgcagcc ctgagcaagc
    15421 catgccggaa ctgcctgaag tcgaaaccac tcgccgcggc ctgcaaccgc acgcgctgca
    15481 gcggcgcatc ctcgccgtag tcgcacgcga acgccggttg cgctggccag ttgaagacgc
    15541 ggtcctgcgc gaacccgtcg cgcagcgcat cgtggcgctg gaacgacgcg gcaaatatct
    15601 gttgttgcga ctggaacgcg gctgcctgat ctggcacctc ggcatgtcag gcagcctgcg
    15661 catcgcggcg catgacgaac ctgcggctgc gcacgatcac atcgatctgg aactcgatga
    15721 cggccgccgc atccgcttcc gtgacccacg ccgttttggc gcgctgctgt ggacaaccgg
    15781 cgatccgttg catcacccct tgctggccaa cctcggcatg gaaccgctcg ccgcaaactt
    15841 cgacggcaat gcgctatacg cgctcacgcg cagccgccgc cccatcaaga ccgtgctcat
    15901 ggacagccac cgcgtatgcg gcatcggcaa catctatgcc aacgaagctt tgttccatgc
    15961 ccgtattgcc cccgacttgc cagccgaatc gctttcctta caactggcga accggctggc
    16021 cggagccata agtgaaacgc tgcagcgcgc catcgcggca gggggaagca gcttgcgcga
    16081 tttcgtcggc gcagacagcc agcccggcta cttccaacag gagtatgccg tttatggcag
    16141 aaccggattg ccgtgcaggc gatgcggtac gccggtggtt cagcacaggc aggctcaacg
    16201 cagcacgttt ttctgtcctg cgtgccaaaa aagctgacga caagtgtacg aaaatgcagt
    16261 atattcaact gctattgcca gttcgccgca ggcggggtgc ccgatgaccc gactgcgcgc
    16321 gacacgtgga tgggacatac agtacagcac cggtcggaaa cctcccggtc aggatcggca
    16381 tgcagcgcac agaacacagc gatcgagaca catcaacacc tggccaggcg agtgagcgtg
    16441 caccgcgcaa cgccgtaaat gacttgcagc agcaattgtc ggcggcctgt taacggagac
    16501 tggacatgtc ggacacccat ctgaccgaac actttgccca atatagctac tggcgcgaga
    16561 atctgacgct gcagatcgaa cgttaccgca cctggctgca cagcgaagaa atcagcgact
    16621 ggcaaaccga cctgcgcctg caacatctgg tggacaaact ggccgacgac aaactcaatg
    16681 tcgcattcgt tgccgagttc tcccgcggca aatccgaact catcaacgcc attttctttg
    16741 ccgatttcaa aaaacgcctg ctgccctcca cggccggccg caccaccatg tgtccgaccg
    16801 aactgctcta cgacccggca cgtccgcccg cgatccagct gctgcctatc ggcacccgcg
    16861 caggcaacgt cagcatcacc gagttcaagc gttaccctga cgaatggacc acactgccac
    16921 tcgataccag ttccgcagac accatgctgg aagcgctcgg caaagtcagc gaaaccatgg
    16981 tcgtcagcca ggaagaagcc gaacgttatg ggctgtttca cagcgacagc cccgatgccc
    17041 aactcaatct gcgtccagac ggctcggtag aaatcccgtg ctggcgccat gcaatcatca
    17101 acttccccca tccgctgctc aaacaggggc tggtgattct ggatactccc ggactgaacg
    17161 ccatcggtac cgaacccgag ctgacgttca acctgctgcc gaatgcgcac gccatcgtat
    17221 tcatgctcgc cgccgacacc ggtgtgacca aatccgacat cgacatctgg cgcaaccaca
    17281 tcgccaacct gcaaaacggc caccgcggcc acctggtggt actcaacaag atcgacggcc
    17341 tgtgggatga cctgaaatcc ggctccgcga ttgcggccga gatccacaat caggtgatca
    17401 gctcggcagc attgctcggc gttgacagca cacagattta tccgatctcg gcacagaaag
    17461 gactgctggc caagatcacc gatgatgacg cactcctgga gaagagtggc ttgccgcgcc
    17521 tcgaggaagc gctgagcgaa caactcattc cctccaagca ggagatcgtg cgtgacagca
    17581 ccagcggtga aatcagcgac ctcgtcacca acacccagga tctgctcacc gcgcggctgc
    17641 acagcgtgga cgaacaactg gaagaactca tcggcctgcg cggcaagaac gaagaagtcg
    17701 tgcagcacat gatgaagaag attgaagagg aaaaagccca gttcgaacgc ggactgcagc
    17761 aatttcacgc gctgcgcagc gtcttctccc agcactcgaa ccagttgttc agctacctgg
    17821 gcatggagcg catcaaaagc gaggtgcatg acacgcgtgt agccatggaa aaaagccgct
    17881 tcagtgccgg cctgcagtcg gccatgaacg agttcttcgc caaaatcagg cagaacatca
    17941 gccagtcatc gctcgccatc gacgaaatca aggcgatgat gagcgcgatg taccgcaagt
    18001 ttcgcctcga acacgggctg gcggagatta cgccgcccaa ctattcgacc ttcaagtaca
    18061 agaaggaaat ggatcggctg gaagacagct acaacaagca tttcaacacg ctgtacaaca
    18121 tgctgaccac cgaacagtac acgctgaccg ccaagttctt cgaaacactg gccagccggg
    18181 tgatcaacat ctatgaagtc gccaaccgtg aggccgacag ctggctcaaa gccatcatgt
    18241 ccccgatgga aagccaggta cgctaacacc agatgcagtg gcgacgccgg ctggaaagca
    18301 tcaaacgcat tcaccgcgcc actgatacgc tcgaagaccg cattgccgaa ctgcaggaga
    18361 tccgctcggg catcctgaga caactgggca acctgacgca gatccgcgac gaaatccacc
    18421 acgcgctgac gccgccacag atggctgtgg cgcaagccgc ctgaaccgtg tgcctgacgc
    18481 tgccgacgac cgtgacacgg ccacctcacc tggctgggca cgcccgaacg catcggatgg
    18541 tgatgcgcac acctgaagcg cccgatcagg ttgcctcgta caccacctgt ccgtccacca
    18601 gcgtatagcg cacctgtccc tgcacctcgt aaccgagaaa cggtgtgttc ttgccctggc
    18661 tgcgcaaggc ggcgcgctcg acccgccacc aggccgaggg atcaaacaca caaatgtcag
    18721 cgacctggcc catggccagt tgcccggctg acagtcccag aatgcgcgcc ggccgtacgg
    18781 tgatggtttc cagcgcgcga gcgagcgcca caccgtgatc ggcggcccac ttgagcgcaa
    18841 gcggcagcag caattcgacg ccggtggcgc cggcctcggt ttcgccgaaa ggcatctgtt
    18901 tggcatcgtc atcaaccggg gaatggtcgg aacacaacgc atcgacgacg ccttcggcca
    18961 ggccacggcg aatcgcgtcg cggtcgcgca gcgtgcgcaa cggcggcacc aggtgatggt
    19021 tcggatcaaa atagccgatg tccatttcag acagatgcag gtgatgcgca ctcacgtcgc
    19081 aagtcaccgg caacccctgc gccttggctt cgcgcaccat gtcgatcgac tgcgcgcatg
    19141 acaggcggca caggtgcacg cgcgccccgg tctcgcgcac cagcagcagt atggtcgaca
    19201 ctgcaatggt ttcggccgtg ccgggaatgc cgggcaggcc gagccgtgag gccaccatgc
    19261 cgtcatgcgc cacccctccc ctggccagcc agccatcctg cgggcgcagc cagacggtaa
    19321 agccaaaggt tgcggcgtat tcgagcgcac gcaacagcac ctgggtgtcc tcgatgccgg
    19381 catcagcctg cgaaaaaccg atgcagccag cgtcacgcaa ctcggccatt tcggtcaacc
    19441 gctcacctgc cagtttctgt gtcagcgcac ccaccggata cacccgggca cggttgaggt
    19501 tgcgtgcgcg gaacttgagc atctcgacca gcccgggttc gtcgagcggc ggatcggtat
    19561 ccggcggaca cgccagcgag gtcacgccac cggcgcaggc cgcatccatt tccgattcca
    19621 gcgtagcccg gtattcgtac cccggctcgc gcaggcgcac cgacaggtcg accagtccag
    19681 gcgctacgat gcagccagcg gcatcgatca cccggttggg aacaaatccc gccaccggct
    19741 cgcccagcga caccaccttg cccgcagcca cactcacatc acccacccgg tcgagaccgg
    19801 acgccggatc gatgatcctt ccctgcctga tcagtatctt caccctagtt tcccgccaac
    19861 atgctcatga ctgccatacg caccgcgatg ccataggtca cctgcggcag aatcaccgat
    19921 tgaccgccat cggccaccgc cgaatcgatt tcgacgccgc ggttcatcgg ccccggatgc
    19981 atgacgatgg catcgggccg tgcatgcgcc agtttttccg ccgtcaggcc gtaatacttg
    20041 aagtactcct gcggactggg caggcgcgca ccacgcatgc gttcattctg cagccgcagg
    20101 ataatcacca catccacgtc gcgcaagcct tgggccaggt cgtggtacac ctgcactccc
    20161 atctggtcga cgccggaagg caacagtgtg cgcggcgcga tgacccgtac ctcgggaacg
    20221 cccagcgtgg tcagcgcatg gatctgcgaa cgtgccacgc gcgaatgcag cacgtcacca
    20281 acgatcgcca cacgcaggtt gtgaaattcg cccttgtagc ggcggatggt aaacatgtcg
    20341 agcaacccct gggtcgggtg ggcatgccgt ccgtcgccgg cattcacgac atggatatgc
    20401 ggcgccacat gacgcgcgat catgtgcgca gcacccgact gcgcatggcg caccacgaac
    20461 atgtcggcct gcatggcggt caggttatcg acggtatcca gcagggtttc gcccttgctc
    20521 tggctggatg cgctgatatt gagattgatc acgtcggcgg acagccgctt ggcggcgatc
    20581 tcgaacgtgg tgcgcgtgcg cgtggatggt tcgaaaaaca ggttgaaaat cgccttgccg
    20641 cgcaacaagg gcaccttgcg cacttcctgt tcgttgatgg agacgaagcc ctcggccgcg
    20701 tcgagaatgt gcatcagcag actgcgcggc agaccttcga tgctcagcag gtgccgcagg
    20761 ctgccatctg cgttcagctg tggattggcg ctcatcaaac ctctaccagt ttcagtgtca
    20821 gctcgccgga ttccaggcga accagatgga tgtgctggtg ggcgggcacc ttgatcgcag
    20881 cgccggcata atcggcggca atcggcagtt gccggtcacc gatgcgatcg atcagcaccg
    20941 caagccggat gcgctcgggg cgaccataat cgaacagcgc gttcattgcc gcacgcaccg
    21001 aacgccctgt atacagcaca tcgtccacca gcaggatgtc ccgcccttcg acttcgaacg
    21061 gcaatcgggt cggccgcacg gtcgcatgca gccctttctg ctcgaaatcg tcgcgataga
    21121 acgaaatatc caggctgccg tgcggcatcg cagggtcaag caacggttgc agacgctctg
    21181 ccgcccacac gccaccggta tgcatgccaa ccagcacggt gttgccggtg agcgaggggc
    21241 gcatgtgctg cgccagctgg tcgatcagtt gctgcgcatc aggcaggttc atgagcgcac
    21301 tccggttcgt caaaatagtg ttgcagaata tgctgtgcag ccacctgatc aagcatgact
    21361 ttctgccgcc ggccgcgcac gcccccttca ttgagcgagg cgctggccgc atgcgagctg
    21421 tagcgttcgt ccatccacac ggtacgcagg ccaaagcgct cgcgcaccct gcggccaaaa
    21481 cgcaggcagg ctggcgccag ttcgtgtgcc ctgccatcat catgcgacgg caggccgatc
    21541 accgccagca ccggtttcca ttgttgcaac agttcggcga tgcgagcgaa gcgcacggcg
    21601 ttgacttcgg aatgaatggt ttccagcggg tgcgcaaggc cgatctcgat gtcgcctacc
    21661 gcaatgccga tccgcttcat gccgaaatcg aacccgagca cgctgccgtg ctgcagctca
    21721 ggcatgtccg gcgctttcgg acagactggc ataatcgacc cccagcagat gcaccgcagc
    21781 gtccagtctc gactcggctg gaaggtcaaa aatcacccga ctgtctgccg ccaccgacaa
    21841 ccatgcattc tgtgccattt cgccttcaag ctgcccggcg ccccagcccg catagccgag
    21901 cgccacgatc attttttccg gcccgccgcc ctgcgccgtc gcatcgagta tgtcacgcga
    21961 tgtcgtcagc gcgatctgct cgcccacgga cagcgtagac tgccagttac cgactggctg
    22021 atgcagcacg aaaccacgcc cggtttcaac cgggccgcca aaatacagcg ggctgtggcc
    22081 gaagccgtca tcctcggcac actgcacacc gatctgactg aacagttcgg acaggttcat
    22141 gtcgatcggg cggttgacga tcagccccag cgcgccttct tcgtcatgct tgcagacata
    22201 ggtaagcgtg cgcgcaaagc gggaatccgc catgcctggc atggcgatga ggaaatgtcc
    22261 ggtcaggtcg atggtatgca tgcgtcaatt gtaacggcgc tgaccgagaa gtacaatttc
    22321 ggtcgcagtc cggattcagc aaaccctggt cggtttgatg ctatcataag cagccctttg
    22381 caccgcatct tccgttggca acgatgaacc agacgcgcac cgatccccag atcaccgcca
    22441 tcaagctgcc gccgcactcc gtagaagccg aacagtcggt actgggcggc ctgctgctcg
    22501 acaacaatgc atgggaccgc attgccgatg tggtcagttt cgaagatttc taccgccacg
    22561 accaccgcct gatctatcag accatcagca aactgatcgg catgggccgg ccggcagacg
    22621 tggtcacgac cggcgaggca ctggaaaatc tcggcgaatt gggcactgtc ggcggactac
    22681 cctacctggt ggcgctggca cagaacacgc catcgtcggc caacatccgt cgttatgcgg
    22741 aaatcgtgcg cgaacgcgcc atcctgcgca agctggccga gaccggcagc gaaatcgccg
    22801 aatcagccta tcagcccgcg gggcgctctg ccaatgagct gctcgaccag gcggaaaaga
    22861 aagtttttga catcgccgaa gccggagccc gcagtcagcg tggcttcatc gacatcaagt
    22921 ccttgcttgg ctcggtggtg gagcgcatcg acgagctgtt cagccgcgag aaccagagcg
    22981 atgtgaccgg aattcccacc ggctacaccg atctggacca aaaaaccgcc ggcctgcagc
    23041 cgggtgacct gatcatcgtc gccggccgcc catcgatggg taaaacggct ttttcgatca
    23101 acattggcga aaatgtcgcc gttgaccacg gcttgccggt tgccgtgttt tccatggaaa
    23161 tgggcgccga acagctggtc acgcgcatgc tcggctccat cgggcggctc gaccagcatc
    23221 gcctgcgcac cggccagctg cgcgacgacg actggacgcg cctgacctac gcgctcggca
    23281 agctcaacga cgcgccgatc tacatcgacg aaactcccgc actcaacgcg ctcgaactgc
    23341 gcgcccgcgc ccgccggctg caccgccagt gtggcaagct gggcctggtc atcatcgact
    23401 acctgcaact gatgtcgagc gtgacctccg gcgaaaaccg cgctacggaa atctccgaaa
    23461 tctcgcgctc gctcaaggca ctggccaagg aattgcacgt accgatcatc gcactgtcgc
    23521 agctcaaccg ctcactggaa cagcgcccca acaagcgccc ggtcatgtcc gacctgcgcg
    23581 aatccggcgc catcgaacag gatgccgacg tgatcctgtt catctaccgc gacgaagtct
    23641 acaaccccga cagcccggac aagggcagcg ccgaaatcat tatcggcaaa cagcgtaacg
    23701 gtccgatcgg catggtgcga ctgaccttcc ttggcgagca tacgcgcttt gaaaatcatg
    23761 ccggcggcga gtacgatgcc gcgcacggca gccatcgttc atgacccgcc cgctgcaggc
    23821 catcatcgac accggcgcct tgcgccacaa cctggatgtg ctgcgccagc aagcggcgca
    23881 cagccgtatc ctcgccgtca tcaaggccaa cgcctacggt catggtctgg ccatcgccgc
    23941 cgacgcgctg gcccaggccg acggcttcgc ggtcgccagc ctcgaagaag cgctgcaact
    24001 gcgacaattg ggcgtggaat gcaccatcct gctgctggaa ggcgtgttcg aagccagcga
    24061 aatccttgcc tgtagcgaac accgactggc gatggtgatc caccggcacg agcaaatcga
    24121 atggctgcgc gccgcccggc tgaaacagcc ggtcgaaatc tttctcaaac tcaacagcgg
    24181 catgaaccgg ctcggattca cgcccgaaac ctatcaccag gcaatggccg aagtccgcgc
    24241 cattgcggca gtctcgcaca tcacccagac cagccatttc gcttcggctg acgaagccga
    24301 tggcatcgaa gcccagatgg cagtcatcca cgccgcgttt tctgcccatg cacatccggc
    24361 aagcctggcc aactcggccg cggtattccg ctatcccgaa agccaccgcg actgggttcg
    24421 ccccggcatc agcctctatg gcggctcgcc gtttgccgac accccggccg aaacgctggg
    24481 gctgcggccg gtgatgacgt tgcacagccg catcatcgcc gtgcagaccc tgcaacccgg
    24541 acaggcactc ggctacggtg gcgactgggt ggcacaacac cccacccgta tcggtgtggt
    24601 agcatgcggt tatgcggacg gctacccgcg ccacgcagga accggtacgc cgattctggt
    24661 caacggacag cgtacgcgca cactgggccg cgtttcaatg gacatgctgt ttgccgacct
    24721 cagctgcgta caaggtgcac aatgcggcag cccggtcacg ctgtgggggg atggacttcc
    24781 ggtcgaacag gtggctcagg cggccggaac catcaactac gaactgctgt gcgcacgtgc
    24841 ccagcgcgtg ccggaacgca tcatctgaga ccggatagcg cagcgcctga acatctctgc
    24901 aagcgaggcc gacatcgcca tggcgaaaga aaccgaactc aagctcgcac tgccacaaaa
    24961 ggctgccgcc cgcctgcggc gacacccgct gctgcaatca gtcactccgc accggctgcg
    25021 actgggcaac acctactacg acactgcgga tcgcctgctc gaacaagaca agatcgccct
    25081 gcgcatccgc cgcatcggca accagcgcct gcagacggtc aagcgggcct caccggctgc
    25141 cggcggattt tccgtacgca gcgaatggga aacccccatg accggcagca ccttcgaatt
    25201 ttccaccatc gaagacccgg cattgcgcaa tcgactcgat gccctgcgtg accagctcga
    25261 accggtgttc agcaccaatt tcacccgtac ggcgtggatc gttcaccccg accccgacag
    25321 cgaaatcgaa ctcgcgcttg atcgcgggga gattcttgcc ggtacccgcc atgaacgcat
    25381 ctgcgaaatc gagctcgaac tggtacgagg ccaacccgcc gtactgttcg aactggctcg
    25441 ccaactgcaa cacgatcttc cgctgcggcc gctctcgctc agcaaggccg aacgcggttt
    25501 cgcgctgcta cacgaccgga cgacggctgc gcgcaaggca gacgccatcc atttcactcc
    25561 ggatctggtc cccgtcgagg cttttcgcgt tattgccctg tcttgcctgt cacaccttca
    25621 ggacaatgaa gcctgctggc tcgccaatcc ggacgacatg gaagcgttgc accagatgca
    25681 tgtcgcgctg cgccggctac gatcggcatg gcgtctgttc gagccgatgc tggagccgac
    25741 gttcaacaca cgctggaaac cttttctgcg tacactcaac ctcgcactcg gccgggcccg
    25801 caactgggac atgcttatcg gcgaactgct accgcacctg atcgcagaag ctgcagaccg
    25861 tgaacacggc cagagattac gcaatgactg cgagcaacgg cgcaagcgtg cccgccggca
    25921 aattcgcacg ctgctccaga ccacggcata tgcgcatttc tcgctcggtc tgaccgcaga
    25981 attgtatgat gggtcattcc acggccgggc tgacacggca gaagctggca ccaccgcaca
    26041 cacgttgacc gcattcgcca gcaccagact caccctggcg gccagcgccg caggcgaacg
    26101 cgcgcgcatg attgacactc tcgatgccga agaacgccac cggctgcgca tcgcctgcaa
    26161 ggaactgcgc tatggcgttg aattcttcgc aacgctatac ccgccggaaa ccgtcctggc
    26221 ctatctgcac acgctcgaac ggttgcagga actgctggga ttgctcaacg atttcgaagt
    26281 cggccgtaaa ctcgttgcca gcctgcgacg aaacggcgaa ttcgaccgca accgctggct
    26341 ggcacaccat cgtcagcact ggcgcaaccg tttggcaccc gctgcgcagg aatttctatc
    26401 cacggccgtc ccgtggcaag tcacctccac gtgacatgac attcatttgc aagtcaggat
    26461 tggcctcttg ggacgcggtg attcattcca cgcgatccgt ggagcattaa caggccgttg
    26521 aaaaaggtag cgaggacgcc cggatgcaag gcgcgagaaa cgcaacgaca agacatatca
    26581 aatagatagg cgtgggagtg agtatcgcgg caacgccgca gacgggtgcc gcagtacttt
    26641 ttcaacggtc tgttaatcac cgcgtccctt gatttccatg tggtgcatga tgcaatccgg
    26701 cccacagcct ggtttcaacc ggatgctttc ccgagtcggt cggcctcgct caatcacaca
    26761 acttgacccg tcccgccgca gatcgcccaa atgaaaagcg ccccgtacgg ggcgcccgaa
    26821 tccggcaaga acctgtggtt tatttggcag cagcagccgg ggcagcggcc ggggcagcag
    26881 cagccggggc agcagcagcc ggagcggcag gcggcgtgaa tttggcgccg ccagcatcag
    26941 ccatataggc cacggcgcgt tcgatttcga gatcggagag gtccgggttg ccaccacggg
    27001 caggcatctg gcgaataccg ttttcagcat gggagatcag cgtcgcgtaa ccttgcttga
    27061 tgcggggacc ccagtcaccc ttgttgccaa acttcggcgc acccagcgcc ccaggcgtat
    27121 ggcacgcggt acatacggcg ttgaacacct gctcgccggt caactcgacg tgcacggcat
    27181 tggcatcggc cacgacaagc gtgccaacag gtttgatacg tgcatctacc gcagcttcga
    27241 ccgattcggc ctgcttcgcg gtatcaggaa tatggctggc ctgcaccttc atcaccagcc
    27301 ccacaatcag aataatcgca accagcgggg caaacaaccc gccaagaact gccatgatga
    27361 actgcatcgg cgtggttttg cccatcttga tgtgattatc actcatcgtt gcacccttac
    27421 taagaaattt tggtt
//
LOCUS       DAIANL010000063        22398 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3135426, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000063 DAIANL010000000
VERSION     DAIANL010000063.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 22398)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22398)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22398
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3135426"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4678 a         7488 c         6230 g         4002 t
ORIGIN      
        1 acgccggcaa ggcgcgcatc caggacgttc tcatcaacgg ccaggaactc atcgtccagg
       61 tagaaaagga cgagcgcggc aacaaggggg cggcgctgac cacttacatc agcctcgccg
      121 gccgctacct ggtactcatg cccaacaacc cgcgtggcgg tggcgtatcg cgccgcattg
      181 aaggcgaaga ccgcaacgag ttgcgcgacg tgatgaacca gctcgaactg ccggccggaa
      241 tgagcatcat cgctcgcacc gccggcattg gccgcggcgt ggaagaactg cagtgggatc
      301 tcaactacct gctgcaactg tggaccgcca tcgacaccgc tgccagcggt cagaagggcg
      361 cattcctgat ctatcaggaa agcagcctgg tgatccgcgc gatccgcgac tatttccagc
      421 ccgacattgg cgaaatcctc atcgatacgc ccgaagtgtt cgaacaggca cgccagttca
      481 tgagccacgt catgcctggc aatgtcaaca aggtcaagct gtaccacgac gacatcccgc
      541 tgttctcgcg ttttcagatc gaacatcaga tcgagaccgc ctacgcccgt gaagtcaacc
      601 tgccgtccgg tggcgcgatc gtcatcgacc acaccgaagc actggtcgct gtcgacgtca
      661 attcggcacg ctccacccgc ggcgcagata tcgaacagac cgccttcaac accaacctgg
      721 aagcagcaga agaagtggct cgccaactgc gtctgcgcga cctgggcggc ctgatcgtga
      781 tcgacttcat cgacatggaa aaccagcgca accagcgcga ggtcgaaaac cggctgcgcg
      841 acgcactgca ttacgaccgc gcacgggtac agttcggcaa aatttcgcgt ttcggtctga
      901 tggaactgtc acgccagcgc ctgcaaccgt cgcttggcga atcaagccac cagccctgcc
      961 cgcgctgtag cggcaccggc tttatccgtg gcatcgaatc caccgcactg cacatcctgc
     1021 gcatcatcca ggagcagtcc atgaaagacc acaccggggc gattcatgca cagatcccgg
     1081 tagatgtcgc cactttcctg ctcaacgaga agcgtgccga catcgtggcg atcgaatccc
     1141 gactcaaggt cagcgtggta ttgattccca acatccacct ggtcacgcca cactacaaga
     1201 tcacgcgttt gcgccaggac gaaatcagcg cccaggacca gctggtgccg agctaccagc
     1261 tctctgaaac gattgaggaa acccagcccg ccgacgcccg caaggaaaac cgcacaccgg
     1321 tccaggtacc cgcggtgcat ggcatcacgc ctgcacaacc ggcaccgatc gtacccgaac
     1381 ggcgccccga ggcagaagcg gctccggtta caacaccggc ccaggccgcc acaccggggc
     1441 gcaagagtct gatcacgcgc atcttcgatt ggctacaaga tcgcagcgaa gaccgccgcg
     1501 caccggaaaa agcagccaca ccggctcccg caccaacgcc acagcccgcc ccgacaccgc
     1561 gcaaccgttc tgcgcgtggt gaaccaggcg agcgacgcga accgcgcgag ccgcgcgaac
     1621 ctcgtgaacc acgtcgcgac ggccgccaac agtccgagcc agggcagcgt cgcgaacgtc
     1681 cgcagcgtcc cgaacgcgaa gccggtgaac cgccagcagc cgctacgaac gaaaccgcaa
     1741 ctcgtggcga aggtacacga cgctcacgtc cgccgcgcca gccccgcccg caggtagaag
     1801 ccggtgtcga agcaacacag gcaccgactg cgaacaactc ggcgaacacg ccacgtgccg
     1861 acggtgccgc ggctggcgaa caggaacagt cctctccatc ccgcagccgc cgcagccggc
     1921 gtaaccggcg cgaacgcagg cctgagacga caccggctgc cagcgaaacg ggcggcgaaa
     1981 tgatcaatga ggagctgaac gaggctgcac cggctgcggc aagcgcaacg acaccgaccg
     2041 ttgccggcga aacggccgca caaccagccg ctgtctcgct ggtgcgcgac accgtaccgc
     2101 gcatcgactt gccgggtgct gaaaccatgc agcctgcagc cgaaacagtc atccccgtgc
     2161 aacccggggt tgcggcagca tccgcaccgg ccacagttgc gaccgaagtg gctgcaccat
     2221 ccgcaccggc cacggttgcc accgaagtgg ctgcagcatc cgcaccggcc acggttgcca
     2281 ccgaagtggc tgtaccatcc gtaccggcca cagctgcggc cgaaacggct gctcagcccg
     2341 taccggcgcc ggtcagcacg cctgccggca ccctggcggc agctgcaaca gtcaaagctc
     2401 cgatcgagca actgctggcc gagctgttgg ccccttcgtc ggaaacggcg gaactgcaga
     2461 tggtggaaac acgcagcgca ccgaccgagt ccgtcgaacc cgagccggta gtggcacgac
     2521 agccgcgtcg ccgcaggtcg gctccggcag tgacgcaggc cgtggaagag ccgttgcagc
     2581 aggtcgagac ccgatcgacg ccagacgcat ccggatcgca acagcagtaa aaagtccacg
     2641 cataccgccc tgcaactgca gggcggcatt gcatggtcag ccctccagcg cctcccagcg
     2701 ctcgagtgca gcttccaggg cttcggccac gcgtgacagt tcgagttcga gcgccacgcg
     2761 acgtgccgga tcgccccgat acaattccgt atccgccagt tgggcagcca gtccggactg
     2821 atcctgctcc agccgcgcga tgtcgttcat gacctgttgc agatcgttcc ccgcgttgcg
     2881 tttcgactct cgcctttgca ccggtaccgg cgcaaccgcc ggcctggcag ccagcttctt
     2941 cgccagcgtt gtgtcttcac cttgcctgtt gcctggcgcc tgctctgcca gcctcgcccg
     3001 ttgcgccagc cagtcatcat agccaccgac atactcgcgc aactgcccat cgccttcgaa
     3061 agcgattacc tgggtgacga tgcgatcaag gaactcgcgg tcatgcgaca ccagcaatac
     3121 cgttccggca taatcctgaa gcagttcttc cagcagttcc agtgtatcga tatcgaggtc
     3181 gttggtcggt tcatcgagca ccagcacatt ggccggccgg gtaaacagac gggccagcag
     3241 cagacgatta cgctcaccgc cagacagcga tttgaccggg gaacgtgcgc gttcgggcgc
     3301 aaacaggaaa tcaccgagat agctgatcac atgcttgcgt acgccgccga tctcgacatg
     3361 atcgctgcca ggactgatga tatcgatcag cgtcgcctcg tcgtccagct ggctgcggaa
     3421 ctggtcgaaa taagccactt ccagccgcgt gccacgctgt acctgcccgg catcgggaac
     3481 gatctctccc agcagcagct tgatgagcgt gcttttgccg gcgccgtttg gcccgatcag
     3541 cccgaccttg tcgccgctgc ggatgagcac actgaaatcg cgaatcaccg gtttgccgtc
     3601 gtacgacttg cttacgccgg taagttccgc cacccgtttg ccggaacgtt cgcccgcatc
     3661 gagcgacagt tgcacctgtc ccagatgatt gcgccgttca ctgcgttgca cacgcaactg
     3721 ttcaagccgg cgtaccctgc cttcgttgcg cgtgcgccgc gcttcgacgc ccttgcggat
     3781 ccagacttct tcctgcgcca ggaacttgtc gaaccgtgcc tgctccaccg cctctaccgc
     3841 cagttgttcg gccttgatct ggcggtaggc ggaatagccg ccttcgtaga agcgcaggcg
     3901 accacggtcg agctcgacga tacgcctggc tacacggtcg agaaaacggc gatcgtgggt
     3961 aatgacgacc acgctgccag gaaattcacc gatcagcgtt tccagccatt cgatggatgc
     4021 cagatcgagg tggttggtcg gttcgtcgag caacagcaca tccggctcgc ccaccagcgc
     4081 ccgcgccagc gccacccgtt tgcgcgtgcc tcccgacagc gcagcgacac gcgcatcggc
     4141 gtcaagcccc agccggctga gcacgttttc gacccgggcc tgcaaccgcc aaccatcggc
     4201 cgcttccagc cgcgtctgta actgctgcat ctgttcgagc agggcgatct gatccgcatg
     4261 cgcgtctgcc agccgcaatg tcaggccgtg atagtcaacc agcaaccggc agacttcacc
     4321 caatccctcg gccacggcct cgaacacggt actgtcggca gcgagcaccg gttcctgggc
     4381 aacaaacgcc agccgcaggc ctggctggcg ccacagcgtg ccatcatcgg gttcaacgat
     4441 gcctgccagc accttgagca ggctcgactt gccggcacca ttacgcccga tcagccccag
     4501 acgggctccg ggctcgataa cccatgccac ccgatcaagc agcgcgacgt gaccaaaggc
     4561 cagcgataca ttgtccaatg tcagcagcgg catggcgtca gcgcccctcc cgcttgccgg
     4621 tgagcgcgac aatcccttcg ttggccagcg catcggctcg ttcgtttccc ggatgcccgg
     4681 cgtgaccgcg cacccagttc cagccgatgc gatgcccgga tgccgcggtg tcgagctgct
     4741 gccacaagtc gacattcttg accggggagc cgtcactcgt acgccaaccg cgccgcttcc
     4801 agcccgacag ccactcggtc atgcccttga ccacgtactg ggagtcggca tgcacctcga
     4861 cacgactcgg ctgcttcaga ctttgcaacg ccacgatcac cgccatcagt tccatgcgat
     4921 tattggtggt catcggctcc gcgccacata attccttgcg atgcgcaccg ctgatcagca
     4981 gtgcgcccca tccgcccact ccgggatttc ccttgcatgc tccatcggta taaatctcca
     5041 cctgcggacc ttctttctca ctcatccctg ctcccgctct gcagcgcccg ggacgccggg
     5101 cgcaacaatc ttccaagatc gcgacgatcc tgccagtccg gcaaaatcag tcgcatgccg
     5161 tgcacccgct tcaccgcatg cacgaaatat acgccgcctc ccttcgccca ccagtgctcg
     5221 cctgccgtct cgagccattc gaaccagcgc acccaccccg tttgtgccaa aggcggcaca
     5281 aaacaacagc tgcggcagtc ctgctgctcg cagccgatca gcgacagcca gtcgcgcagc
     5341 ctgtgcagac tgacgaaacg gtcctcccag acgaaattgc cccgggcacg cttgtaccgg
     5401 taatacaccc cccacaggct ccacgggttg aagccactga tcagcagatt gccctccggg
     5461 atcagcacac gttctgcttc gcgcagaatc tggtgcggat aggggaaaaa ttcaagcgca
     5521 tgtggcaaca ccagcaggtc gatcgactga ccggcaaacg gcaaacgtgc cggatcggcg
     5581 agcaggtccg cgcctaccgc aacgtcaaga cgatagcggt gcttgatccg gctcgcgcgc
     5641 agcagatcga cattgcgcag cccgacctgc accgcattga agccgaacag gtcggccgtc
     5701 atggcatcaa ggatgatctg ctcacgactg cgcaggtatt ccccgtcagt gctggcaaac
     5761 cattctgcaa aagaactgta acggtcggtc atgctatcat agcgccaatg gatactgaaa
     5821 ttcatgtcag cccgatcccg gcatttcggg acaattatat ctgggccatt cacaaccggc
     5881 atcaggcagc gctggtcgat ccgggcgatg ccgcgccggt acgcgcattt ctcgagcgca
     5941 acggcctcac cccctgcgcc atcctcaaca cccaccacca ccacgaccat atcggcggca
     6001 atgccgagct ggtagcgcaa tacggcatcg acgtctacgg cccggccgac gaggacatcc
     6061 ccagcatgaa ccgtccagtg cgtgaaggcg acaccgtcac cctcgatgca ctgggcatcc
     6121 ggctacaggt catcgacgtg cccggacaca ccgccggcca catcgcctgg cacgctcccg
     6181 aacagcacct gctgttcagt ggcgacacgc tgttcgggtg tggctgcggc cgcctgtttg
     6241 aaggcagtgc cgaacagatg tatcactcgc tggaaaaact ggcccggctt ccgggcgcga
     6301 cccgggtgtt ctgcgcccac gaatataccc tgagcaatat tgccttcgcg ctcacggtcg
     6361 aacccgacaa tgccgcactg caggcccgcg cgcgcgctga ccgcgaacat gtggccaggc
     6421 accagcccac gctgccatcc acgctggacg ccgaactcgc caccaacccc ttcctgcgct
     6481 gtcatctgcc acaactgcga cggcatgccg aattcagcag cggacgcatg gttgacagcg
     6541 cgaccgaagt attcagtata ctacgcagtc tgaagaacga gtttcgttga cctatgcatg
     6601 cgccgatccg ttccaacatg acagcagcat gggccttgcc gaatgaaatg caaggcgtat
     6661 ttcgcgaccg ggaagcaatg cgcccgacac cgaagtcatc gtggcaattc gccagcctgg
     6721 ccacacctgt cgattacgtc cagtccggcc ggctgccgtg ctgcacgccg gcacatcgtg
     6781 ttgccggtaa cggcctgtgg cgtacgcagc acactcttcc cgggaccgac cgtcatcctg
     6841 cctgtggcgc aataaatcgg caattccctg ccaccgcgac cgtaagcagt ctggtcatcc
     6901 gctcctgccg acagctgctg acgtcggcaa tgctgtttct gggtgtcgga ctgtttgcag
     6961 cgggtgcctt tgctgccgga ttgcccaccg accttggcac cgacgttacc gccgaatcct
     7021 cgacttcgat gctgcagcag tggatcggca ccgagccgct ggccgcctgc cgaccggacg
     7081 gcagcgacac gcccatggcc agtgatgcgt ctgccggagc cagcctcgcc ggcaccagca
     7141 cgcccagcac cagcctcgcc agcaccggca cgcccggcac cggtctcgac gccaacaccg
     7201 ctgtcgccag acccgaagac ctgtggcaac acttgcgcga aggttttgcc ctgacccctg
     7261 agtcaagccg gcgtgtagac aaccagctga gctggtacac ccaccggcca gactatatca
     7321 accgcatgat cgatcgcagc aagcgttacc tgcactacat cgccagcgaa gtgaagaaac
     7381 gcggcatgcc cagtgagatt gccttgctgc cgatggtaga gagtgcctac aacccgatgg
     7441 cctactctgc cagccacgcc tcgggcatct ggcagttcgt gccccagacc ggcaagaatt
     7501 tcggcttgcg cgagaccagc tggtacgacg gacggcgcga catcgtcgca gcaaccaatg
     7561 ccgcacttac ctacctgacc cgactgcacc agcagttcgg cacctgggaa ctggcgcttg
     7621 cagcctataa ctgcggcgaa ggttgtgtac agcatgccat cgcccgtaac gaatcgctcg
     7681 gcttgccgac cgactatcag gacctcaacc tgccggacga aacccgcgat tacgtgccaa
     7741 aactgctggc catccgccag atcgtcagtg atccgcaagc caacggcatc agcctggaaa
     7801 gcattcccga caaggcttat ttcaccaccg tcacactcga caaacccatg gacgttgccc
     7861 tggccgcacg ccttgccaac atgccggtac acgacttcct ttcgctcaac ccgggctata
     7921 gccgaccggt catccgttcc gacagcggca tgcaggtatt gctgcccgtc gaccgtgccg
     7981 atacattcac aaccaacctg aaccactaca cccggccact ggtgagctgg cagggctgtc
     8041 cggccaaccg cggcgaatcg ctgccactcg tcgcacgccg ctgccacacc agcgtggccg
     8101 aactcaaggc gcacaacccg ctgaagctca atcgcaaggg ccattttcgc gagtaccagt
     8161 cgctgatcgt accgatccgt ggtcgtgaca acgacggtgc cttcatccag gtgtccatgc
     8221 agcagacgga gaacgctgcc accgacgccg gcccggcgca gcaccatgcc gccgcacacg
     8281 gcaaggcaaa aaaacgcaac gtacatgccg tgcgcaaggc cactgcccac cgcagtgctc
     8341 atacaccagc gaagaaaaag cctcccgtgc gcaaagccca ccaccgcgca tcacgcgtac
     8401 gccatccggt tcactgagtc aggccagcga ccgaccctcc ggcctgtgca tctgcagtca
     8461 gcggaaaatg acaatgcacg atgtgcccac ccgcaaacga tagcgcgctc ggctcggtgt
     8521 gtgtgcacag cgtttgcgca cgtggacagc gcggatgaaa cgcacaccct gacggcggat
     8581 gcagcgccga ggctgcctcg ccccgaatga cgccagccgc tgcctggcct ggacgcggaa
     8641 tcgccgccag caatacctgc gtatagggat gcaacgggcg ctccagcaga tcggcagtcg
     8701 caccggtttc cacgatacgt ccgagataca tgacggccat gcggtgtgcg agatagcctg
     8761 cgagcaccag attatgcgtg atgaacagca aggcgactgc cgattgctgc tgaatgtcac
     8821 gcaacaggtt gaccacctgg gcctgcacag acacatcgag cgcactggtc ggttcatcac
     8881 acacgatcaa ccgcggccgc accgccaacg cccgcgcaat agcgatgcgc tggcgctggc
     8941 cgccagaaaa ctcatgcgga taacgctcga gcgcctgttc aggcagacca acctgggcca
     9001 tcagttcgcg caggcgcccg cgcctgcgcc gcgcatcctc ctcgacgtgc atggccacca
     9061 tcccttcttc cagcaggtgg tcgatgcgca tgcgcggatt gagcgatgcg aatggatcct
     9121 ggaacaccat ctgcactcca gcacgaccga ttctgcgctg gccggcatca gtcgggtatt
     9181 ctgcgctgtc gcgcagcacc aggctgccgg tatctgcggc ctgcaatccg gcaatcaccc
     9241 gtccgagtgt ggtcttgccg catcccgatt cgcccaccag cgccaatgtt tcgccggcat
     9301 gcagatagag gttgacccga tcgaccgcca gcaatgtccg ccgcgcttca aaccagcgca
     9361 aggcactcag ggaaaattcc acactcagct ggtccacctc aagcaacggc gcgccgccat
     9421 tgctgaccac aggcgccgtc acagcagatg tttcagtgga tgcaatgaac gccttttcca
     9481 gtaaatggca acgcgcaaaa tgtccggcgt ccacttcgac ccacggcgca acggtgacga
     9541 aacagcgcgc ctccgccaga tcacagcgct gtgcaaaacg gcatccggaa aaacgggtgc
     9601 cggcctgagg cacactgcca ggaatctgca tcagtcgggc agcactccgt ccgggcaacg
     9661 cagcgaacaa tttgcggctg tacggatgcc gcgcggcgga aaaaaactgt tcacgcgtgg
     9721 caatttcaac cagttcgccg gcatacagca cgccgatgcg gtcggccatg atcccggcca
     9781 cccccaggtc gtgggtgatc agtagcatac ccatgccccg ccgccgctgc gcctcgcgca
     9841 gcagttcgag tacctgcgcc tgcacggtga catcgagcgc ggtcgtcggc tcgtcggcga
     9901 tcagcaggcg cggattcccg gccagcgcca tggcaatcat ggcgcgctgt ttcatgccgc
     9961 ctgacaggcg aaacggatat tcggccagcc gggcggacgc ttcagcgatg ccgactgcct
    10021 gcaacagccc gacggcctcg cgacgcagcg ccttgccgcg caactggcgg tgcaacgcca
    10081 atgattcacc gatctgctct cccagagtca gcaccggatt gaggctggtc atcggttcct
    10141 ggaaaatcat ggcgatttca ccaccacgta acgcacgtac cgcagattca ggtgcatcga
    10201 gcaaatccct cccgcgccag ccgacatgcc cagcctcaaa ccgcgcggcg gatggcaaca
    10261 gacgcatgat cgccagtgca atcatggatt ttccagaccc cgattcaccg atcagggcaa
    10321 aagtctcctg cgacgcgata ccgaagccga cgccatcgag cacacgcgca tcaccgatac
    10381 tcacacacag atcgctgatt tcaagcaatg tttcgctcat gaacggcttc caagcgggtc
    10441 aaacgcctcg cgcacgctgt cggcaaacag gttggccgac aacaccagca cgaacatcag
    10501 gctgaatgcc gcggtcaacg gccaccacac caccggttcg cgtgcgagtt cgagctgggc
    10561 tgcattgatc atgttgcccc agctcggcac tgatggatcg acacccagcc ccaggtagga
    10621 cagcaccgct tccaccagca ccagcgaaga aaagtccatg accacgctga tcagcaccag
    10681 gtgggtgaga ttgggcagga tgtggcgcca catgatgcgc cagtcggaca cgccgaaaga
    10741 gcgcgctgcc tgcacgtatt cgagttcgcg cagcttgagg gtttcggcgc gcagcaaccg
    10801 gcacagcccg gtccagctgg tcacgccaag aatggcgcac agaaacagca ggcgaaaatc
    10861 ggcgcgcagt gccagactgt caaaatgggc ggcatgcact tcgatataac cttgcatcag
    10921 cagtactgcc gcagcaatca gcaaaatgcc gggaatggca ttcagcgtgg tgtagacata
    10981 ctggatgata tcgtcgatcc agccaccaaa atagcctgcg gccaccccca gtccgacggc
    11041 tagcggcagt gtcgccagcg tggtcagcaa gccgaacagg aaatcggtac gaatgctctt
    11101 gagcgactga tacagcacat cctgacccac tttgtcggtg ccaaacacat gccatgaagc
    11161 ggcaagctgc actgtcaccg caaacaacac acaccctacc gtccacatca ccagcgccgt
    11221 gcgccaggcc agcggtggca atgcagctcg cgccgctcgc ctgcggcgaa accagatcag
    11281 gaccgatgcg gcacttgcgc cagccagccc gagtccctgc aatacaccgt accatactgc
    11341 tgcggccggt tgcgcatgcc catgcgcctg ctgcgcaacg atggccaaca gcggatagac
    11401 gcgctgctgg tggccatctg gccgcagcac ctgttcctgc acgaacagcc ggtccgccaa
    11461 cggcgccgaa taggtttttt cgctcacccg ttgcaacggc cgcaatagca gatcaaatac
    11521 actgaccaca ccggaatcgc cggctgccgg atgaaaatgc aacgaatcga gcagcgtcac
    11581 gccaagatag gcgaccagca ccatgccact ggccacggcc accggcgcgc gcgtcaaccg
    11641 tgccaccccc ttgcgcagcc cactgtcgcg ccgcgccagt gcaaatgcaa atcctgcgac
    11701 cagcagccag accagcgcgt cggtccacaa tatcaccgga tgaatgccca tcatgacaac
    11761 ctcacccgcg gatcaaccca gccgtaggca agatcggtca gccacagccc gatcaggtac
    11821 aacaccgtgc cgataaacac catggcgcgt accacggaaa aatcctgcgc agcgatggcg
    11881 tccaccacat acgagcccag accggggata ccaaagaacg attctgtcag caggctgccc
    11941 atgaacagca gcggcaacac cgccacggta ctggtcacca ccggaatcag tccgttacgc
    12001 aacacatgac ggaacaggat acgcgattcc gacagtccct tggcacgcgc ggtgcgcaca
    12061 taatcctttc cagcttcctc gagaaagatg ctgcggtacc agcggctgcc attgccgatg
    12121 ccagacagca cggcaatcag cacaggcagc agcacaaagc gccatccatc gatacctggc
    12181 acaaatccgg ataccggcgc cagtcgccac agacgcgcca ccaggaactg accggccatg
    12241 atatagaaca ggctcgaaac cgacatcagc atggctgcca gcgccacgcc cagacgatct
    12301 ggccaaccag cgcgaaacca gaccatcatc agggccagtg caatattggt ccacaggcca
    12361 atcagaaacg acggcagcgc aatcatcaga ctgggaccgg cgcgagcacg aatttcccgg
    12421 ccaatgtcgc gaccgtcgtc ggactgacca aaatccagtg caaacatgcg tatcgaccgg
    12481 gtatagaaca gggtatcggt ccaggccgtc agccccttcc tgccggcatt gtagaacagg
    12541 ggtttgtcat agccatgcgt gacctcccac tgcgcaatcg ccatcgcagt cacccgtttg
    12601 ttgcccagat gcatgcgcgc catgtcttcc ggtgaattga ccatgaaaaa caaccagaat
    12661 gtcagcagat tcacgccgat cagaatcggt atggcatata gcagcctgcg cagccagtag
    12721 gcattcatcg cgcggtcatc ctgtcacgta tgccgctggc aaaccgggcc gaaaccagta
    12781 ccatcaatac caaccctccc aggacgaata acggccagcg caccggatgg ttccacgcct
    12841 gccgctggcg ggcgcgcagc gccggatcga catgcacata cttgagcgtg ttgttggcga
    12901 tcacgtttgc cttgcccggg ccaacccacc cctgacgcaa catgtactgg cgcgggaagt
    12961 agccaaacat ccagggggca tcgtagcgca ggatactgat catctgctgg atcacccgca
    13021 gccgctcagg cccgtccggc atgtatttca tctggtcgaa caatgcgtca taacgtgcgt
    13081 tatgataatt ggcatcgttt tcaccgttgc tgcccacctt ggcatttggc ccgtaaaaca
    13141 gaaacagaaa attttccgga tcaggatagt cggcgttcca gccgccgaca aacatctgcg
    13201 tgttgccgcg acgcagcttg tcctggaatc ggttgaaatc actgctgcgc gataccatct
    13261 ggatgcccaa ccgggccagc tgcgatgcca tccagtcaac gtatgccttg gcgtcgggtc
    13321 cggtaaaggt attgtcataa tacagcacca gcggtgcccc cgtgtgcgca tcgcgccccc
    13381 cgggataccc tgcttcggca agcaaccggc gcgcatcatc cagggatcgc cgctcgatgc
    13441 gaccatttac cgagtgatac accaccgggt tgatacccgc ctccccgtcc tcgtagccaa
    13501 agatgcccgg cggtatcggt ccttgcgctg caataccgcg tccattgaga aaaatggaaa
    13561 tgaaatcttc ataattgacg gcgatcgaca acgcctgacg cagcttgcgc gcacggtcgc
    13621 tgtagccacc cacgacattg tccagcatgt tgaacgccac gtagtaaata ctggtcatca
    13681 ctgcggtgtc gagttgcatg ccccgccgtg ccagttcggg actgacccgc ggttcccctt
    13741 gcgcatcgag acggatggcc tggtcgaagg tgtccgaact gatcaacgac aggtcataat
    13801 agccctgcat gaatttgtcc cagtacggga tcacttcttt ttccagggta aagacgacat
    13861 ggtcggcaaa cggcagtgac tggccggcat agcgcagcag ccccgcctgc gcgtcgccag
    13921 gatcgccgct gtccggatag cgctcgccat ggaaattcgg attgcgcgac agttccatgc
    13981 gccagttcgg ctgcatgaac gacagcaggt acggtcccgt acccagcgga tgccattcaa
    14041 gcgtgaaatt gtgcgccgcc atgcctggct gatgggtaaa cgcgtccact tcgggcggca
    14101 ccggggcaaa aaacggcatt gccagccaat acaggaactg cggatatttc ccctgcaatt
    14161 cgatccgcag cgtgtagcga tcgaccacct gcaccccggg cagcggatac cgctccaggt
    14221 caaggtagcg atcctgcgac cagcgaccgg ccacgcgatc gagcatctgc gcataggccc
    14281 tgaatccgac gatatagtcc tccatcagac cgaggatggg cgaattgacc tgcggcgatg
    14341 ccaggcggcg aatttcgtgg gcatagtcgg ccgcgaccag ctcgcgcgtg ccgagttggg
    14401 taaaatcccg ccagccacgc atatgcgcca actggtgggc agacagcgga atgtaacatg
    14461 accgtccggc ggcatctcgc gcgagcgccg ggtgcggctg gtagtaaatc cctggccgca
    14521 cgtgcagttc atagatgctg cggtacactg atgcggccgg ggcatccgct ggcaatatgc
    14581 gccctttcgt atcgatcagc gtcaccttcg gcatggcgct caacgtcagc ggaatgagtg
    14641 tgtaaggtcg cttgagatag tgatactgca ggggcggttc gagaatctgg ccgattaccg
    14701 cataggcatc cgaactgtac gcctgcgcag gatcgaggtg cttgggccgc tcgacgaagc
    14761 cgagatagag cgtattgctc tgccattcac ctgcaggata aggatcattg acgaccggca
    14821 cggaacaacc ggtcagtatc agaacaagaa agaggaatac cacacgcacc atggacgaag
    14881 tgtaccgcag tcgggccggg ttgcgaaccg ctgaccccga tcgcgcccga cacccgtggg
    14941 cacccaccag tcaacaggat gcgatctgcc gatgccggcc cgccgtagta ggctttttcc
    15001 ggatgcggag ggtataatgg cggggctttg acaacaagga cagtctcatg ggatttttac
    15061 gtgacaaacg aattctcatc accggaatga tcagcgagcg ttcgattgcc tacggcattg
    15121 cccaggcatg ccaccgcgag ggcgcctctc ttgccttcac ctaccagggt gaaaaaattg
    15181 ctgaacgtgt cgccgaaatg gcagctgagt tcggcagcac tatcgtcctg ccttgcgatg
    15241 tctccagcga cgaggaaatc gaacagctgt tcgtctcgct cggcaaggaa tgggatggct
    15301 tcgacggcct cgtacatgcc atcgccttcg tgcccaaaca ggcactggcc ggcgattttc
    15361 tcgaaacgat gaaccgcgag cactttcgca tcgcccatga catcagttcc tacagcttcg
    15421 ccgccatggc caaggctgca ctaccgatga tggaaggccg tcagtccgcg ctggtgtcgc
    15481 tgtcctacct cggcgcggtg cgctcggtgc ccaactacaa cgtgatgggc ctggccaagg
    15541 ccagtctgga aagcagcgta tactacatgg cgcaaagcct cgggccaaag ggcatccgcg
    15601 tcaacgcgat ctcggccggt ccgattcgca cactggccgc cagcggcatc aacggcttcc
    15661 acaagattct caaccatgtc gaaaaaaacg cgccgttgcg gcgcaacgtc accatcatgg
    15721 aagtcggcaa taccgctgca tttctgctgt cggaccttgc cagcggcatc accggcgaaa
    15781 tcacttatgt agacggcggc ctgaacacga ccatgggtgt tgtggaacag taagctcccg
    15841 caccaacttc tcttgcctgc gaacgcgcgc cgcacactcc tgcggcgtgc aatccgcccc
    15901 cccccatcac cgttgcgcca tgcttccagc cctgttctgc cgctcgtctg cgctgttcag
    15961 ggctgattgc cgccgttgac cgcctgccgg tagatatcca ccttggcctt gtcgagcagc
    16021 gaatgctggt aatcggcaaa cgcctgctgg gccagcatgc gcgccatctg gtcctgcatg
    16081 acgcgcagct tgtccgcctc ggcgggaggc gcactgacgc gtgaaatgcg caccagatgc
    16141 cagttaccct gcgcatcctg tgctccgaca tacgccggca gcgtgttttc atcggcgatg
    16201 aataccggac cgaccagttc ggccggcagc gaacctgcct gcgaacgggt cacccattcg
    16261 aacgcactcc acttgagtcc gtccggttcc ttgccggcgc gcaattcacc cagccatgca
    16321 ttgcctttgc tcgccaccag ctggcgcgcc atctggtcac gcaacaacgt ctggatctgc
    16381 ggtgtcacct cagccagcgt ctgaacgcgt gcaggctgat aatccagtaa atgcgctgca
    16441 accagcgtat tgggagtcac ctcgatggct tcagtgttat ggtgtttgac cagcacgtcc
    16501 ggagaaaaca gagcctgcag catttttccg cctgccagca ccgcggtatc gccgccatgc
    16561 tgtgtcatcc acgccgatgt ctgcaacgtc aatccgagac tgtcggccac cggcttgagc
    16621 tggtcagcct gctcgaacac ccggttgctg aagcgctcgg cggcatccgc aaagcgggtt
    16681 ccggcctgct gtttctgcag actggcaaca atggcatcac tggcttcggc cagggtctgt
    16741 gtcttcgccg ggcgtatccc ttcgagctga atgatgtggt agccatattc ggtatgtacc
    16801 ggagcggaaa tctgccctgg ctgcatgccg aacaccgtgt ccgcaaacgg cttgaccatg
    16861 gcatctcgcg caaaccagcc cagatcgcct ccctgcgatg cagaccccgg atctgcagaa
    16921 tactgcttgg ccaacgcagc gaactggtca gggtgggcca ccagcttatg atagatgtcc
    16981 atgaccagtg cccgtgctgc cgcctgcttc tgcgggctgt cgccggcaac cgatgcaatc
    17041 aggatatgcc gtgcctggcg ctgttccggc tggatgaact gcgtcgggtt ctgtgtgtag
    17101 tattgcttca ctgccgccgc atcgatcggg aggtgccttg cgaaatcatc gagggaaagc
    17161 accacatact ggaagcgtgc gcgcgcaggc aaggtaaaat cgcgcacgtg cgcggcatag
    17221 taagcctgaa tcgcctgcgg gctggcggcc ggcaccgaac ctgcctcggc aggcgtcagc
    17281 tcgacctgac tgatttcacg ccgctgccca tatgcctgca tgaacaactg cgcggagctg
    17341 gccggaatct ggcgcgcatt ggcaaatgcc gcacgcatgt cctgcatcat caggtcgtcg
    17401 cgcaccatct gctcgaaagc ggacaccgtc agcccctgac tgcgcaacat ctgttgatag
    17461 cgttgcaggt cgaaatgccc attgctctgg aacgccggga tggtggcgat gaacgcggcc
    17521 aactggcgat cgctgatcac catgcccagc ctgtgtgccg tctgcaccag cagatgctca
    17581 cgaatcaagg aatcgagcac ttcgctgcgc aggcgcggat cgtccgtgac actggcagcc
    17641 tcggcgccca gacttgcagt cagtgcgttt tgctgttgtc gcagcgcgcg ctggaattcg
    17701 ttcactgaaa tgttctcgcc atcgaccgtc gctgcgacgc tgtcgttgct gcgatggttg
    17761 atataggaat tcacgccaaa caaggcaaat ggcaccatga tcagcgccag tatgatccgc
    17821 acgatccagc cttgagtatg ttcgcgtatc cagtccagca tgccattcca cccgttcgat
    17881 gtcagcccgg cccgtgcttt ctgttcagca cgcaggcaaa gcagtgatta tactccaaac
    17941 cattacagcc gatggcgctg gcacggtctg ctcattcacg accggcgacg acgtagcggt
    18001 tcctgccggc cgccttgcca cggtacaggg cctggtccgc acgctggatg gcctgcgcca
    18061 gggtttcttc ctcacctgcc cgagcaacgc caatggtagc ggtcggtcgc accaccgcgc
    18121 cgtccgcaag cgtgacggcg agctgtcgca gcgattccag cacccgttgc gcagtcaaat
    18181 cttccgcgtg actgctcgac gacacatcca taagcaacaa gaactcctcg ccaccatacc
    18241 ggtacaccag atcgttttcc cgcactgcct gcctgagcgt cacggcaatc gcctgcagca
    18301 cggcgtcgcc gcccgcatgc ccatagcggt cattgaccgc cttgaaatgg tccacatcga
    18361 ccatgagcag caactgggca tcaccacgcc gacgcacatg tctggccaac aggtcaaaat
    18421 cctgttccag gcgatggcgc aggggcaacc tggtcaaagg atcgatttgc gagctttggc
    18481 ctacggccag tgtcttgtaa tgggcgagcg cttcgatcag ctgatgctgg gtcgtttcaa
    18541 aacggtcgag atggctggat tcgccgggca gcccggccag catgcgctcg cagataatgc
    18601 cgatcgcggc atgcatggca tggtgcgcgg tttccagcga ctcgatccgg ttggcgtcca
    18661 gcgcggcaaa atcatcgcgg tgtgcagcaa accaacgtcc gaaatgacag cgctggcttg
    18721 agccggcatc caggatttca ttgcccggtg aggtcttgag cacggcacag cgcaacaccc
    18781 tgcgcgtcca ttccagatgc gcctgaacag ctgcgtccag ttcggcgacg aactgctcga
    18841 agtcaatttc acgcatggaa ccgcagcacc tgccggggaa tgactgaaaa tgaaaacggc
    18901 ctgcataaca caggccgttt gacatgctgg cggagtggac gggactcgaa cccgcgaccc
    18961 ccggcgtgac aggccggtat tctaaccaac tgaactacca ctccagatta tcccttggtg
    19021 ggtgctgagg ggttcgaacc cccgacattc gccttgtaag ggcgacgctc taccaactga
    19081 gctaagcacc cgggaagccg gtcagtttac cgcatccttg agggattttc cagcgcggaa
    19141 cttgggaacc ttggctgcct tgatctggat cgccttaccc gtctgcggat tacggccaga
    19201 acgtgcggca cgatcaccaa cataaaacga accaaaaccg accagtgtca caacatcgcc
    19261 ttttttcagc gccgctgtca cggcatcaag cgcggcttcg atcatgcgac ccgctgttgc
    19321 cttggatacg tccgccgatt ccgcgatgac atcaatcaat tcagatttgt tcacttgttt
    19381 ccccttgtgt ttagtttaca gacgtgtcca gcaaagcgct ttataacaag cgaactaacc
    19441 cgcgtcaagc caggcgctgc acaaatgcgc catccggcgc gggttgcacg cggcccaaca
    19501 gaccaaaggc ccaccccgtt acgggcaggc cttcagccaa gctgatgatt tcaatgcttg
    19561 gtcggtgcga tatccgcggc agccgtgacc ggattgagta cttccggcgt cagtaccggt
    19621 tcttccacca acggttccgg ctggcgctcg agcacgatct gcaatacctg atcgatccat
    19681 ttcaccggcc ggatatcgag ccggtttttg atgttgtcag gaatttcgac cagatcacgt
    19741 acgttctctt ccggtatcag caccgtcttg atcccgccgc gatgcgccgc cagcagtttt
    19801 tccttcaggc caccgatcgg caacacttcg ccacggaggg tgatctctcc ggtcatggcc
    19861 acgtcggcac gtaccggaat ccctgtcagc accgaaacca gcgccgtgca gataccaatg
    19921 ccggcactcg gcccatcctt gggcgtagcg ccctcgggca ggtggatatg cacgtcgtgt
    19981 ttctggtaga aatcttccgg aatccccagg gcacgcgagc ggctgcgcac caccgacatg
    20041 gcggccttga tcgactcctg catcacctcg cccagctggc cggtggtggt catgttgccc
    20101 ttgcccggca tcagcacgcc ctcgatggtc agcagctcgc cacccacttc agtccatgcc
    20161 agtccggtga cctgccccac ctggttgtgt ttctcggcca tgccatagct gaagcggcga
    20221 acgccgagga acttgtccag gttgcgcgag gtcacgctga tgcgtttccc acgtccgggc
    20281 ttggtcagca gcgctgtcac cgccttgcgg cagatcttgg ccacatcccg ttccagattg
    20341 cgcacgccgg cctcacgcgt gtagtaacgc acgacgtcgc gcaacgcgct ctcggcaatg
    20401 acgcattcct caggtttgag accgttggct gccagttgct tgggcaccag atagcgttcg
    20461 gcgatattta ctttttcgtc ctcggtatag ccggacagac gaatcacctc catccggtcc
    20521 atcagcggcg ccggtatgtt gagcgtattg gcggtggcca cgaacatcac gtcggacagg
    20581 tcgaaatcga cctcgatgta atgatccgag aacgtatgat tctgctccgg gtcgagcact
    20641 tcgagcagcg ccgacgacgg atcgccacgg taatcctgcc ccattttgtc aacctcgtcg
    20701 agcaggaaca gcggattacg cacgcctacc ttgctcatgc tttgcagtat cttgcccggc
    20761 atcgagccga tgtaggtgcg gcggtgtcca cggatttcgg attcgtcacg cacgccaccc
    20821 aacgccatgc gcacaaactt gcggttggtg gccttggcaa tcgactgccc gagcgaagtc
    20881 ttgcccacac cgggcggccc caccaggcac aggatcggtc ccttgagctt ctctacgcgc
    20941 tgctgcacgg ccaggtattc gacgatgcgt tccttgacct tgtccaggcc ataatggtct
    21001 tcgtcgagaa tggcctgcgc cttggcaagg tcgcggctga tgcgcgtacg cttgcgccac
    21061 ggcagcccca gcagggcttc gatatagctg cgcaccaccg tcgcttcggc agacatcggc
    21121 gacatgagct tgagcttctt cagctcggat tcggccttgg cctcggcatc cttgctcatg
    21181 cgcgcatcct tgatgcgctt ttccagttcc tcgagctcga ccccttcttc accatcgccc
    21241 agctctttct ggatggcctt gacctgctcg ttgaggtaat agtcgcgctg gcttttctcc
    21301 atctgccgct tcacgcggcc acggatgcgc ttttcgacct gaagaatgtc aatttcgcct
    21361 tcgagcagcc ccatcagatg ttccagccgt tcctggacac cgaacatttc cagcacttcc
    21421 tgtttctgtt caagcttgag gggcaaatgg gcagcgacgg tatcggccag ccggccagct
    21481 tcgtcgatgc cgaccagcga ggtgagaatc tcgggcggaa tcttcttgtt gagcttgacg
    21541 tactggtcga actggctgac cagtgcccgg cgcgcggcct cggtttcctt gtcggcgcct
    21601 tcttcatcag acggaaccag ttccacggtg ccgacgaagt gactatcggc atcggcatat
    21661 tcgacgacgc gtgcgcgctg attgccttcc accagcacct tgacggtgcc atccggcaac
    21721 ttgagcatct gcaaaatggt ggccacgcta ccgatacgat acagatcttc gggagtcggg
    21781 tcgtccttcg aagccgattt ctgtgccgca agcaggatat tcttgccgga ttccatggcg
    21841 atctccagcg cgcgaatcga cttggcgcgg cccacgaaca gggggatcac catgtgggga
    21901 aacaccacca cgtcacgcag cggcagaagc ggcagcgtca ttgtttcgac attcgcttgt
    21961 gaggacatca tgtgcaaccc ttctaaaatg gaatcacagg aacacatata caggcgaacc
    22021 ggcgaatttc aagtatcgcc gcgacaaaac cacgcacgga acgccagtcg cgcccgatcc
    22081 gggccgccac acgccccgat ccctgcgtcg ccgacccgac ggcgcaggga ctgcctcacg
    22141 acgccagctt ggcctggtca gtatagatca tcaacggctg tgtctcgcca cgaatcacct
    22201 gtccgtcgat caccaccttg ctgacgttct gcagcgaagg cagttcgaac atggtgtcga
    22261 gcagcgcatg ctcgatgatc gatcgcaagc cgcgcgcgcc cgtcttgcgc gccagcgcac
    22321 gctcggcaat ggcttcaagc gcatcgtcgc ggaactcgag ttccacgcct tccatctcga
    22381 acagacgggc gtactgct
//
LOCUS       DAIANL010000064        13705 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_884731, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000064 DAIANL010000000
VERSION     DAIANL010000064.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 13705)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13705)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13705
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884731"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2477 a         3881 c         4762 g         2585 t
ORIGIN      
        1 accgagagcg accgcaagcc ggacgtagtc ctgcgcctgt tgcatccggg cctgggttcg
       61 ttgcagttgt gcctgcgcga tggcgtgctg cgcgcgcaac agcgccgggc gggcgacggt
      121 gccggcgtgc tggcgtgttt gcgtcagttc aaggcccgct gccgcattgc gtacctcatc
      181 gtcggccagc gcaagtgtct ggcgatcgtg atcgagcgct gtcagcgcat cggcgatctg
      241 gccgaacgcc tgcagcaccg tttgtcggta ctgtgagcgc gctgcctgcc aggcgtgttc
      301 ggcggcgcgg tgatgttcgc gcagcgcgcc gccatcgaac agtggtgccg agatgccggc
      361 ggcaatcgcc caggcgtcgt tggacaggtt gaacagcccg gccggactca atgcttcttg
      421 catccagttg gcgctcagcg tgacttgtgg gtacatggcc gcctttgcca tgccggcctc
      481 ggccgctgcg gcgtgcaggt tggctgcggc cacgagaatg tctggtcgtt gctgcgcaag
      541 ttgcgacggc aggctggccg gcggcgattc cggcagtgaa aaatcgttca gcgacagccg
      601 tgggggttgc catacggcag gcggtttgcc caacaagatc gccagcagat ggcggctgct
      661 ggcgacctgt tgctgcagtc cgggaatgcg ttcgcggtcg tgggtccatt gcgcctcggc
      721 gtcgagcgtt tcctgacggg tggcttcgcc tgcggcgcgc gtctggtgca ccagtgcgac
      781 ggcgtcacgg tcggtgtcag cctgttgctg tgctgcagcc agtgcatcca gtccggcggc
      841 gtagtcgagt gcggtcaatg caatgctggc ctgtatctcc agtcgcagcg catcgagatt
      901 tcgcgcctgg tattcggcca gtgcctggcg tcgtgcaatg gcctggtgga tggcgccgga
      961 gaaatccggt gtccaggcaa tggcgggccc ggcttcatac cagctgaagg gggggatggt
     1021 gaactgggcg ggtccgaaca gcgcagcacc atacttctgt cgtccggcca tggcatcgag
     1081 cgacacctgc ggtagcaggc cgccacgttg tgctgccagc gcatggcggg cttctgccag
     1141 cctttgccgt gccgtttcga ggtcggcgtt gtggttgtcg gcttcgctca tccagtggtc
     1201 gagcgtagcg cagcgaaagg tcgtccacca gtcgccagtc tgcatgacag cagccgtttg
     1261 cgcctgtgac gcgggctgtt cgtcaggggc cagccaggct cgggccggcg gggaggttcg
     1321 cgcatcgggc aggccgggtg aattgctgca tgcggcgagc agcaggctgg ccgtgcatgc
     1381 cagcgccgtg gcctggatgt gcggtttcat tccatcaccg ccagttctgc cttggcctgg
     1441 ctgcgggcag gaggccgcag cagcaccagc gttggcaaca gcagcagtgt gaggatgaac
     1501 atcagcttga agtcgtcgag gtaggcgatc atggtggcct cgcgggtgat catgtgatcg
     1561 agcgccagcc agccacccgg gctgacggtg gcgatgtggc tggccgccag cgcaagcgcc
     1621 ggatggtgcc agggcgcgaa atggcttgcc agtcccgcat gtacggtttg cgtgttgcgt
     1681 gtgagcaggg tttgcacggc ggaaatgccg atggcactgc cgatgttgcg ttgcaggttg
     1741 aaaaaggcgg tgccttcatt gcgcagtgag gtggggagcg tggcgaatgc caccgtggaa
     1801 atcggcacat aggcgaggcc gatgccaaag ccttgcagga ttccggagac gacgatcggt
     1861 tcctcgctca tgagcagatt gaaatccatc atctgccaca gcgacaccag catcagcatc
     1921 agccccaggc caacgatcca gcgtgcatcg atacgtccga tcatccgccc gaccaccatc
     1981 atgccgagca tggtgccggc accgcgcggt gccgtgacca tcccgctggc aattaccgga
     2041 taggccagtt cgttctgcag catgggtgga atcaatgcca gggtggcgaa caggatgacg
     2101 ccgatcatga aaatgaatac gttggcggtg acgaaattgc gatcggcgaa cagcgcgagg
     2161 ctgaggaacg gccgcgggtg ggtgagtgaa tgcacgagga aactgtacat ggccagtccg
     2221 gcgagcaggg cttcggcgat gatctccgga gaactgaacc agtccttgag ttcgccgcgg
     2281 tcgagaaaca actgcagggc tccgataccg agcgcaagcg aggtaaagcc gaaaaagtcg
     2341 aatcgtcccg gctggcgcac ggtttccggc atggatgccg acaagccgac aaaggcaatg
     2401 atgccgaccg gaacgttgat gaagaacacc cagttccagc tgtagttttc cgtgagccag
     2461 ccgccgagca tgggaccgag taccggaccg agggtgacgc ccacacccca gatcgacatg
     2521 gcgcgtccgt ggtgttcggg tgggttgatg tcaaacagca ccgcctgcga cagcggtacc
     2581 agcgcggcac ctgacatgcc ctgcagtacg cggcagagga cgatcatggc caggttgccg
     2641 gatagcccgc ataacgcgga actgacggtg aagccgacaa tggagatcag gaacacgcgc
     2701 ttgcggccaa agcgtccggc cagccagccg gtgagcggaa tcatgattgc tgcggcaatg
     2761 atgtatgagg tcagcaccca catcatctgg tcctgcgtgg ctgacagcga tccctgcatt
     2821 tgcggcagtg caacattggc aatggtcgag tcgagcgcct gcatgatggt ggctgccatg
     2881 atggaaaacg tgatcagcgg ccgattgagc ggtttatcgg ccagtcgtgc gtgcggtgga
     2941 tggggtaccg tcgcgccttc gccggcttcc tcgatgctct ggtcgagcag gtcgtccgga
     3001 gtctgtgcca tggtcagaaa tcttccagcc ggctgtggcc agtgtcgacg ctgacctcgg
     3061 cgctcaggcc ggtatgcacc gggaggtcgc cgtgaaagtc atcgatgcgg atgcgaaccg
     3121 gcagacgctg caccaccttg atccagtttc cggaaacatt ttccggcggc agcagcgcaa
     3181 aactggaacc ggtgccaggg ctaaggctgt cgacccgacc atggaaccgg cggccgggaa
     3241 tggcatcgat ttcgatgatc gcggtctggc ccggccgcat gtgggccaga tcggtttcct
     3301 tgaagttggc ttcgacccag gtctggtggt tcagcaccag gccgaacaca gccgcgccgg
     3361 ctttgatgta atcaccacgc tgcagattgt ccacctgggt gacgatgccg tcgtccgggg
     3421 cacggatgac cgtgcgtgcc agatcgaggc gggcgcggtc gagttcggcc cgggccagtt
     3481 gcaccgcagg ctgctggtcg accggcagtt ccggatggcg gtcgagcagc gccagggtgt
     3541 tgtcacgtgc gtgaccgagg acagcgacct gttggcgtgc ggcgtcgagc gcatgcgcag
     3601 cgctgtccag ttgtgccggc gaggcgatgt catgctgggc caactgcttc tgccgggcgt
     3661 actcgcgttg ttgccaggcc agtgtttgcc gggcggcgcg tagttcggca tcgcgttgat
     3721 ccagcgtggc gcggagtgag cggatatgca gttgcgcgtc ggccagtcgg gcctgggcaa
     3781 cgcgttcagc gagaacgaaa tcgcggccgt ccagggcaaa cagcgggtcg ccgcgtcgta
     3841 ccggctggtt gtcgtgtacg tagacccgca ccacgcggcc gtcgatatgc gcgctgacat
     3901 cgacatgcgc cgcctgagta taggcatcgt cggtcgacac cgagcggccg ccggccagat
     3961 agaatgccgc gacgataatc agcaccagca gcgggcccgc gatcagcaag cgtcggcgca
     4021 gccgttcgga ggtgttgttg gacttgctca cgggagtact ccgtcaatgg gtgccggcgc
     4081 gtaaagctgc cttgaggcga tcattgtagc gaagcttgat tgcaaggata atactcgata
     4141 atccgaatgg attatgtgtt tttatcgaat aatcgctgcc agcgtgtcgg agggcaatgt
     4201 gcggggtgct gaattcgccg aattgcgggc attcatggaa gtggccgagc atggcaactt
     4261 cggtcgtgcg gcgacccggc tcgggctggc gccatcgacg ctgagtcaga cgatccgggc
     4321 gcttgagacg cggctggaga cgcggctgct gcatcgcagc acgcgtagcg tttcgctgac
     4381 cgaagccggc cgccatctgc tggaacggat tcgcccggcg tttggcgagc tggaagccgc
     4441 cgtggaatca atcaatgctt ttcgtgccgt accgagcggt aatttgcgcc tcagcatatc
     4501 aaccgtgccg gcacaaatga tcctggcgcc cttgctcagg gattttctgc atgcctatcc
     4561 gggcatccgg ctggaaatcg aggttgatcg cgccaatgtc gatatcgtca agggtgggtt
     4621 cgatgcaggt atccgttacg ggtcgtatat cgcacgcgac atggtagtga tgcaggtgag
     4681 cccgccgacg cgtatgttgg ctgtggcgtc ccctggctat ttgcaatgcc attccgtgcc
     4741 gacgcaaccc catgatttgc gcaaccatga ttgcatccgc ttccgttcgg tcaaccggca
     4801 gacgctggtg tgggagttcg aacaggcagg gcggcgtatc gaagtcgatg tcgaggggcc
     4861 gctgctggtt aatgacgcgg acctgctggt gcgtgcggtg cgtgacggca ttggcatcgg
     4921 ttacatggcc gaggcgtata tgcgagaaca cctgctcagc ggcagcctgg tgccactgct
     4981 ggccgactgg gcggtgacca tggatggctg gttcctgtat tatgcaagcc ggcatcattt
     5041 gccagcaccg ctgaaagcgt tcatccagtt tttgcgcgat cgacaggtgg ggaacggtga
     5101 cgccgggcgt gtgctgccag actgacggag ggagaagcgc atgggtgaca ggatacggac
     5161 gtgtctgcgc gaaacgcgct ggactgtaac cgccaacgtg gcggggcagg cctgtgatcg
     5221 ggtgcggctg gcggcgcaac aggcgctgga ggctcgggga gtgttccacc tggtgctggc
     5281 cggtggttcc acaccgcgcg cactgtatgc gcaactggct ggcctgcaca ccgactggcg
     5341 tggctggcat ctgtggttcg gtgacgagcg ttgcctgcct gaacacgatg cccagcgcaa
     5401 cagtcgcatg gccgacgcag cctggctggc agccagcccc atcccgccca gtcagatcca
     5461 tcgcatgcac gccgagcgcg gtgcggtggc aggcgtgcgc gactatgtcg gccaactggc
     5521 gggaaccgaa acgttcgatc tcgtgctgct cggcctgggc gaggatgggc ataccgccag
     5581 cctgtttccc cgacggttgc ccgagccaga agatgccgtg gcaattgccg tcatggatgc
     5641 acccaaacca cctgccgaac gcatcagtct cagcgcgcgc aggctgtcgg atgcgcgtgc
     5701 cgtactgttt ctggtgaccg gggcgggcaa gtgcgatgca gtgtcacgct ggcggcgcgg
     5761 cgacgcgatt ccggcggccg agatctgtcc accgcatggt acggttgagg tgctgctcga
     5821 gccggtgtgc ctcgccgaag cgcagtcgca gatctgagtc ccggcctcag gccgcggcgg
     5881 ccgtgggtgt cgcgatcgtg gcgctcggat tggcgcgtgc ctgatggcgc tggtcgatcc
     5941 agttgcgtgc cgcaatgatg aggccaagcc cgaagaatgc gcccggcggc aggatcgaca
     6001 gcaggaaccc cggatagtca ggaatgacgt ggattaccca gtgtctggcg atcgggccga
     6061 gagcgaggtc gatgcccgat agcagggtgc ccttgcccac cagttcgcgc aatgcgccca
     6121 gcatgaccag caccaccgtc accccgagtc ccatcatgaa gccgtccacc agcgagggca
     6181 gcaccggatt gcgcgaggcg aatacttcgg tgcgtgccag cacgatgcag ttggtgacga
     6241 tgagcgggat gaagatgccc agtaccaggt agagcggatg cacgaaggca ttcatgctca
     6301 tgtcgatgat ggtcaccagc gcggcgatga tgaggatgaa taccgggatg cgcgtgtccg
     6361 gagtcacgtg gttgcgtacc agcgatacgc cggcgttgct gaatgccatg accagggcgg
     6421 tggcgatgcc cagtccgagc gcattggtga tgttggtgct gatggcaagc aacgggcaca
     6481 tgccaagaat ctgcaccatg ctcgggtttt gtttccataa gccattgacg atgatttcac
     6541 ggtaggcgct catgaccgga ctcccggatg ggtggaaaat aacagttggt ggtgcaatgc
     6601 tgcgtattgc agggcatgat gcaccgcctt gatgactgcg cgcggcgtga tggtcgcgcc
     6661 ggtcatgtag tcgaactggc cgccgtcttt cttgacctgc cagccagtcg tatcgggttg
     6721 ttgcagggac ttcccatcaa atatacgtat ccagctactt tttgcaagtt caatgtagtc
     6781 gcccagtccc ggtgtttcat ggtgttcgac cacacgcacg ccggtaatgc ggccatcggc
     6841 gccgataccg accagcaggt tgatcttgcc accataaccg tccggtgcgg ttgcctccag
     6901 caccacggcc actggctggt tgttttcgcg ggcctgccag cccaggctgg acggaccatc
     6961 attgtcgagg agcggatcgg cgggcaggat gaactggttg gagagcagat cgttgttgta
     7021 actgccccga accagggttt ccgcgaccag tttacgtcgc gcatcgtcct cgtttttctg
     7081 gattgccagg tgcgtggcgt cgtagacaaa agcaagcacg ccactgccaa tgatcgcgta
     7141 ggtcagcagc aggccggccg agcgcagcgc ggaacggatc gattcgttca tgtacgctgc
     7201 tcctgcttgt ggccataggg cggtggctgg gtccaggcgt cgacgagagg cacggtgata
     7261 ttcatcaaca gcacaccgaa tgccacgcca tcggggtagg cgccaaattc gcgaatcagg
     7321 tagaccagca ggccgatgcc ggcagcgtaa atcagtttgc cgcgtggcgt cgtggcggca
     7381 gttaccggat cggtggcgat gaaaaatgcg ccgagcaggc tgccgcctgt ggcgaggtgg
     7441 aaccatggac tggcatagtg ttgcggctcg atcagccaga atattgcgga aacaagcgtc
     7501 agtgcaccga gcatgcctgc cggaatgtgc caggtgatga tgcgctgttg cagcaggaac
     7561 aggccaccgg ccagataggc cagcgcaaca tattcgctgc ccttgccgcc gatggcgccg
     7621 aagatgggca tttggcggat ggcgtccacg ctgtggtgca gcttgagttg ggtgcgcagc
     7681 gtgtcgagcg gtgtggcgct ggtgatggcg tcaagtttcc agccatcagg cagaccgccg
     7741 ccaaaaatgt aatgtgccgc atcggccagc gacaggtggt ttccggtcag cagagccgga
     7801 gcgggccagt gcgtcatggg caccgggaac gaaatgatga gtaccgcgta ggccaccatg
     7861 tccggattga aaatattgtt gccaagtccg ccgtacaggt gcttggcgac gatgatggca
     7921 aaaaagatgc cggtcagtgt cagccaccag ggcgcaatgg caggaatcga cagtgccagc
     7981 agccaggcgg tcaccagtgc gctgccatcc gccaggccga ccgtgaccgg acggccgcgc
     8041 aaggtgagcg ccaacgcttc gaggccgagc gccgcagcgc tggcaagtgt cagcgacacc
     8101 aggatggcgg gcccgaacag ccagacctgg actgcaatgg caggcagcag cgccagcgcc
     8161 accctgaaca tgatgctggt gacactgttt ccggtcagga tgtagggaga ggactgcatg
     8221 gtttactcgg tcggggaatg gttcggatca tcctgcttct ggctctgggt gcgttcgacg
     8281 gcagcctgga tggcactttt ggccgcgctg gcattcatgt cggtggtcgg tttggcagcc
     8341 gatttctgtg ccagtcgggc caggcgttcc tgctcatcgc gttccaggcg gctgaggcgg
     8401 aattcgtggc gttcgcgcgc atggttggcg gcgcgttcgg cgctgtgctg cgtggaaatc
     8461 tcgcccttgg catagcggta gtagtgcacc agcggaatat ggctcgggca gacgtagctg
     8521 cagcagccgc attcgatgca atcgaacagg cggtattgct ccgccttgtc gaaccggtcg
     8581 ttgcgggaaa accagtacag ttcctgcggt tgcagttcgg cagggcaggc gtcggcgcat
     8641 cgggtacacc ggatgcattc ctgtgctgac ggcgctggtg gaaacagccg ccttgactcg
     8701 accagtatgc agttgacggc cttgaccacg ggtgccgagt cgtcagccag tccgaagccc
     8761 atcatcggac cgccgatcac atgaccgctc accgcttccg gatggccgcc ggccagggcg
     8821 accaggtcgc gtattggtgt gccgatgcgg gtttcgaaat tctgcggctg cccaacattg
     8881 ccggtgacgg tgacgatgcg cgagatcatc ggttcgccga attcgacggc acggtgaacc
     8941 gccagtgcag tgccgacgtt gaaggtggct acgccgatat cggtcgagcg cccgcccgag
     9001 ggcacttcgc gtccggtgat gactttgatc atctgtttgg cgccgccact cggatacagc
     9061 gtgggaatga cggcgatctc cacagcgatg ccgcaacggg ctgcggcatc gcgcatggct
     9121 gccgcagcct gtggcttgtt gtcctcgatg cctaccacca cttcgtccga ttgcagcaga
     9181 tggcgcatga tggcggcgcc gcgcagtatg ccatcggcac gctcgcgcat ggtgagatcg
     9241 tcgcacgtga tccatggttc gcattcgccg ccattgagca gcagcagggg caccttggac
     9301 accttgcccg ggttgagctt gatgaaggtc ggaaatactg cgccacccag gccagcgatt
     9361 cccatgtcgc gcagcaggtt gcgcagcgtg ctgggggggg tattgagata gtcggtcggc
     9421 gcatgctcga tccagcggtc ctcgccgtcc ggctcgatgt ggatgcacag gtcgcgcaac
     9481 ccggaagggt gggccacagg atgaagttcg atggcgatca ccttgcccga tgtcgaggca
     9541 tgcactgcga cagaaacgta gctgtcgggg cgggcaatga cctggccctt gagtacggtg
     9601 tcgccgacgg cgacgcaggg gcgtgctgct tcgccggtgt gctgtagtac cgggatcacc
     9661 aggcgctgcg gtaacggcgc gatggcgatg ggccgcgtgc aggatacggt cttgtgttct
     9721 tcaggatgga cgccgccgtg aaagcggtgc agggtgcgat tcataccggg atcaatgtgc
     9781 gggggtcagg atggtgacgg ggtatttcca tttccagttg gaaacatttt cgccgatcgg
     9841 caccatgtgg atgcaatcga ccgggcaggg ggcaacgcac agttcgcagc cggtgcattc
     9901 gctggcgatg acggtatgca tctgttttgc ggcgccgacg atggcatcga cagggcaggc
     9961 ttgcaggcat aacgtgcagc caatgcagac attttcgtca atgatggcga cggattttgg
    10021 tttggttgga gcagcatcgt cgccgaacgg cttgaattcc acgcccagca gttcggccag
    10081 tttgcgaatg ccggcttcgc cgccaggcgg gcagcggttg atgtcggcct gatggctggc
    10141 gatggcttcc gcgtaggggc gacagccggg aaatccgcac tggccacact gggtttgcgg
    10201 caggatggca tcaatgcgtt cgaccagtgg gtcttcctcg acacgaaaac gcacggcggc
    10261 atagcccagc gtcagaccga gcagtaccgc cagcagcgac atgaccagga tggccccgat
    10321 catggcttga ccattccggc aaatcccatg aaggcaagac tcatgatgcc ggcggtaatc
    10381 atggcgatac tggtgccgcg gaagatggcc ggcatgtcgg cgccctcaag ccgttcgcgc
    10441 aggccggcga acaggatgag cgacagcgaa aagccaaccg aaccgccgaa accatagagc
    10501 agcgactgga cgaaatcgtg ctgcgcctgc acgttgagca gcggaatgcc gagcacggcg
    10561 caattggagg tgatgagtgg caggtagatg ccgagcacgc gaaacagtac cgggcttgcc
    10621 cagcgcacga acatttcggt gaaaccgacg atgccggcga tgacgacgat gaatgataac
    10681 gtgcgcagat aggcgagatc ggtgccaagc aggtactggt tgatgagata actcgtgccg
    10741 gagcccagcg tcaggacgaa ggtggtggca gcccccatgc cgatggctgt ttcaagtttt
    10801 ttcgacacgc caaagaacgg acacatgccc agaatcttgg ccatgacgat gttgttgacg
    10861 aagactgtgc cgatcagaat cagcaggtag tcctggatgt tcatgagtgc ccgttcgtgt
    10921 gctcagtgca ataagactga ttatccgtct tgcccgccgg tttttcaaca cgcatatcag
    10981 tatttcagag gatgtttatc cgttttgtcg cttagcggag gcggtgccgg aagagactgt
    11041 tcaccgtcga aacggggcag gaaggagtcc gctggagcag gcagcatgtt gcccggttgc
    11101 gggtcaaggc tgaagtcata aggctggtcg gtcgactgtg tggcatcgag catattggcg
    11161 aggtgggccc aggcggcgaa gccgtccggc cggtgcgggc cactgcgaca cgcctgcaca
    11221 tcgagccaca gcagaatgac ccggtttgca gtgtagccca ggctccactg gccgcagccg
    11281 gattgcccgc tcagccaggc tgcgcgggcg ctgaccgccg cagactgcaa cgcaggatgg
    11341 ttacgcggat cggcgagtaa ttgcgaaagt tgcgccacgg cgtcgctctg cagcgtcggc
    11401 atgcggtctg gcaggccacc aggacggaag ctgagcgggg tctccacgcc atggttggcc
    11461 agcgtctgcc aggcgctgcc cagggcaagc aggttgccca cgatcggctc cggttggccg
    11521 ggttgtgcca gcccgagccc gaatgaaggt agcagtgcgc tgaaagctgg cgctggcacg
    11581 gcgtcgagta cgcgccatgc ggccggatag ttgccatcgg tcagcatgcg tgcgaccgtt
    11641 attgttgtct ggggtggctg tggtgttacg ccggggcttg ttgcagggtc gggcaacagt
    11701 ggcaatggcg aagcatcgat catctggtca ggctgcagcg taccgcgttc aaaggcgagt
    11761 tcgaccagta gcggccatgc ggcctgatcg agcagccgtg gctgcatgag cggatcgctg
    11821 ctggctggct gggcatgtcg tgtcgcccag gcgaggattt cgccactgtc cgccgagatg
    11881 atcagtaccg caccatcgct gtaaggcccg ttttgtggca tggcttcgtt cagcgcctca
    11941 agggatgacc gttgcaatga acggtcgaga ctggtgacca gccgctgacc cggttgcgcc
    12001 agcaggtaac gggctgcatc cgatgcgagg cccggtgtct gttgcaggtg tggtgggtgt
    12061 gtcaggtaac gcgcagccag ggcgcgaata tgctggcagt cgggctgtgg tgcgctcatg
    12121 cgatgcgcga gtgtgcaggc gcgactggcg accacgcttg cgggaacgtc cggtccacgc
    12181 aacagcgcgg caagcagggt ggcctgggcc gggtcgatcg cagagggcgt ggtgccgaac
    12241 agcccttgtg tcgcggcatg gatgccggtc aggccggctc ggaagcgcgc caggttgaga
    12301 taggcttcga ggatctgcgc cttgtcccag tgtcgttcga gggtttgtgc ggcggcgatc
    12361 tgacgcagct tctgccgcac gctcctgccg ccggtttcgc gcgacaggcg tgggtcgagc
    12421 aggcccgcca gttgcatggt cagggtggaa gcgccgcggt gggtgttata gaacaggttt
    12481 tcccaggcgg caccaaacac ggcttgccag tcgacaccat ggtggcgaaa gaagcgctgg
    12541 tcttccgaaa tcagcaaggc ctgctgcatt gccggcgaca ggctggcgag tggtacccag
    12601 tcaagccggc gggcgccggg cgtgagccgt attctttgca gcagttgacc gttgtggtcg
    12661 agcagatcgg cgtagcagga gggatgagct tcacgcacct cgctgaaggt gggcagggcc
    12721 gcgactgccg gcagcgtgtg cgccagtccg gcaatcaggc cggcaaggac gagagcgcgc
    12781 gtacgcattc gccgaccagt gccgggccgc ggtagatcag tccggtgtac agttgtatca
    12841 ggcttgcgcc ggccaggatc ttttcgcgcg cggcctcgcc gtcgagaatg ccgccagcgg
    12901 cgatgatggg cagttcgccg tgcagtgcgg cggcaagccg gcgcacgatg gtggtggcat
    12961 ggctgcgcag cggtgcgccc gagagtcctc cgctttcctc cgcatggggc aggtcgcgca
    13021 cggcatcccg cgccagcgtg gtgttggtgg cgatcactgc atcgatacgg tgacgacgca
    13081 gcaggtcggc gatggccgac agtccgggtt cgtcgaggtc gggtgcaatc ttgactgcaa
    13141 ccggcacgta gcggccatga cggtcggcaa ggcgggactg agcggatttg atggcggaca
    13201 gcaggccatc gagggcgggg gcagcctgca ggtcgcgcag gttgcgggta ttgggagaag
    13261 aaatgttgat ggtgacgtaa ctggccagtg cgtagacggc ttcgaatccg gtcaggtaat
    13321 cgtcggttgc gcgttcgatc ggggtgtcgg cgttcttgcc caggttgatg ccaaggatgc
    13381 cgcgatagcg ggcggcgcgg acgtgctcgg ccagatgttc cacgccgtgg ttgttgaatc
    13441 ccatgcggtt aatgagcgct tcggcctgtg gcaggcgaaa caggcgcggt cgtgggttgc
    13501 ctggttgcgg gcggggcgtg acggtaccga tttcgagaaa accgaagccg agcgcggcca
    13561 ggccatcgat gcactcgcca tctttgtcga gaccggcagc caggccgacc ggattggcaa
    13621 aatgcagtcc catgacttcg acgggtgcgc cctgccagta cggtcggggc cacaaggcgg
    13681 tgagaccggc gcgttcggcc agacg
//
LOCUS       DAIANL010000065         6764 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1909585, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000065 DAIANL010000000
VERSION     DAIANL010000065.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909585"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1512 a         2159 c         1884 g         1209 t
ORIGIN      
        1 cctccacgag cagggtatgg gagatcttga aactcatgat tcgctcctct catgatgtgg
       61 gcaaactgaa cacaggcgca cttctggatc aagatcggtt ctgcacgcgc gacgagtgca
      121 atcatacgta atttttctgt atgcaaacag gaattgctgg aaaatttcaa atacaggaga
      181 aaattgaatt taagctgaat tcatttttat tcaagtcagc atgttatggc atattcctgc
      241 cgacagtgac atcaggtgca aacgcaatgt caacaagtgt ttataaagat gaaatgttcg
      301 gttacgggca taggcgtccg tgcgtttgat ccgcgctcgc cgggaaaatc atccgcgcaa
      361 cgtggtatgc gcatgaacag gtcgttcggt gctggctgat gctgccgcgc ctgcgcagca
      421 gcatgtaagc caggggtgtg aggggaatgg ttggggaccg gctgttactt ctgtgaaccg
      481 ctttgctgtg aggatccacc accgcttaca gcgtgcttga attccttgcc gccttccttg
      541 gcggcttcgc caatggcctt gccgactttc acggcatcgt gggcgatggc tagcccggtc
      601 tgtttcgcac cctgggcaat attgtgtacg gcctcaccgg ttgcgtgtcc agcctgtttc
      661 gcatcctggg taagcgtctc atcggcatgc gcaactgcgg ctgcagccat gaacgcaatc
      721 gcacccgcac agataacact tctgacgagc agtcgggaac atacgacatg acgactcatt
      781 gcaccactcc ttcaaacaat cagttcgtca gactccgcca cagccggaca gttccttgca
      841 tcgcggatag cgccgctgca gcacaatcag gctgatccaa tttcgttttc gcctgtattt
      901 tgcacaacgg aaatgcaagc cagaatcaaa cggcctgcca agactgccgc ccgccggggc
      961 agatcaggcc tacaactggt gccggcagca agaatcgaac tcgcgacctg atgattacaa
     1021 atcaactgct ctaccaactg agctatgccg gcgtgaggcg caattatgcc attgcaagcc
     1081 ggtccgggtc aaaagccacc ccgccagcgc aaccagacac gcaactgacg caccgcttcg
     1141 gcatcggcat tgtcggcaag cagcaccagg tccacccgtc gtccggcaca cagcagattg
     1201 accacggtga gcaactcggt aaccaacgct tcctgcaccc gtgcatcgag ccaacggccg
     1261 cgccacaaca ctcggcaacc gtctgcatcg gcttcgaaaa cactcaccgg cggatggcac
     1321 tgacgacgat catgccacac tgcactaccg ccaagagcgg tcagaacggc ggccagcagg
     1381 taagggttgc cgatcgccag ccagcacgag tacgcaccca tgccatgcgc cagcgccaat
     1441 agtgcgcgca ggcgcaacga cgatccgatc tcgacccgaa aacgattcac cagccgaact
     1501 ggcggcagaa ttgccccagc cgttcggccg cctccgcgtg ttcaaaaaag cgatcggcgc
     1561 ggttgagccg tgcggcatcg cgctcgtcag cactgccacc aaaacagaaa atcggcacgt
     1621 tgagcatcca ctccatgcgc accttgcgaa ttgcatcctg cggatcgaaa ccgttgtcca
     1681 gccaatcgag atcgagaaag atgaaacggt acaatagcag ctggtcaaaa ctgccaagtt
     1741 cgtccagatc aagcatcacc tgcatttccc agtccggcgg caatgccgca cgcacggtgg
     1801 cctgcagttc atgattgccc gtcatgcaca gcatcttgtg ctgcagacgt gcggctatgg
     1861 tcggttcagt catgatcatc acttccatcg ttgaaacagg cggtttccag cacctgttgc
     1921 gcccgacgcg ctgcctcgtc aggcgacatc cagcacgcca gcgttgccgc aagcgcctct
     1981 ggcgatacgc ccgcctgggc cagcgcggat tctggcaact gcggacaggc cgacaccgca
     2041 attcgttcca gttgcccatc acgcgtcaag acacgcaccg tatccgcacc gaactggcgt
     2101 tcggcaagaa aacagccggc tcgcagcttg atgccgtcac gcacgcgccg acgtacgggc
     2161 agcatctcgt cggacaccac atcttctcgc cacgcttcgc gcgcggcccg ctcgtcatca
     2221 cgcaacagtg cctgttccca gcgccagcca aaacgcgtgg ctgccgcccg acgaaaatgc
     2281 agtgacagcc acgcaggatc gggcacgcta ccctgctcgg cccaactcgt catctggctg
     2341 cgcaatgctt cgcgcaacca gtcgcggaaa ccgggtgagg gtgccgccac ggcggcggcg
     2401 aaggcatcgt aggggaaatc aagcagaaag ctgctgccca ccaatccggc atcgccgatg
     2461 gtggcggcgc cactgccagc cagcttgcgc tggtgcagcc acacgtcctg acctacgcgc
     2521 cgcaccggcc agcccatgtc ggcatacaca tcgagcaagg gagcaagcgc gtgaccatac
     2581 cagtccgccg tgcgcaccgg gaaaaatcca cgcggtgcaa ccagcaccag acaggcttgc
     2641 gaacggtcga tccacacccc gccgccgccc agcggtcggc gcacgacagt cagaccatga
     2701 cccgattcca gttcggccat ggccgactgg tgctgcccca gacaaatatg cgcctcaggc
     2761 cagccccaca ccagcaccgg atcatcctgc ggccgcatgc aatcggccac ggcagcgtag
     2821 gtggcatgaa aatcgaccgc gcaacactcg ccaagatcga tccagcgcgc tttcagttgc
     2881 cggatgatga ctctcccgca gcggcattgc ccgccctgaa gaacttgccg aaatagaaat
     2941 cgcgccggtt cagcgcatcg agttcttcag cgcgcgcggt caggcctgcg cgcatgatgt
     3001 cggccagata ctggccttgc tcggcatcca cgatgtggat cagctcataa agcgaatcat
     3061 cgatatcgac gaaactggcc tccatgccct gcaacggctg ggcatacacc agccaggcac
     3121 ggcactcgct gtcttctgga tcgagcgggc tcatcacgat cttgccgagc caggccagtc
     3181 gggctgcctg gttgaacgtc tggatatcca gctttttgcc tttcttgtac ttgttgagct
     3241 cgttcttgat cgcgccgaca tcggttacct tgatcgattt catggtctgt ctcctgtatt
     3301 aaccgccacc ggggcgatgc aaaatgattt ccagcacgaa gtgtgtcccc agatatgcca
     3361 gcagcagcat ggtaaagccg aaccaggtcc atcgcaccgc cacccgaccg cgccagcccc
     3421 acagtttgcg accgccgagc agaaccgcat acacccccca cgacaacgcg gcaaatactg
     3481 ttttatggtt gaactgtacc ggttcgtgga acagttcttc cgagaagagc atgccgctga
     3541 tcaacgtcaa cgtaagcaat gtgaagccga caccgatcag acggaacagc acactctcca
     3601 gagtcagcag gggtggcagg cgtggcatat cgggactggt cccgatacga tgcaggcgtt
     3661 tttccaccac cgacatcagc gaggcatgca gcgccgcgat ggtgaacagg ctgtaagcca
     3721 gcagcgcaat catcaggtgc aggcgaaacg ccggctcatc ggaattgtgc atcaggtgca
     3781 ccgacggcag caaccacggc gcaaacaccg ccatggcagc aaacatcgcc agcggcgcat
     3841 gcagcgcttc catgcgcatg tgaaagctgg ccagccagta gaccagcacc gtcagcgcgc
     3901 cgatcatcga caccacgtta ccgaccccca gctgcagccc ttccggcgta gccacgaccg
     3961 cgtgcaacag cagaaagtgc agcgtgagcg gcagcagcag ggcaaacggc tcccagcggc
     4021 ggcgcacatc caccgtcacg ccttgcggaa gggctttcca gcgtgtacgc cagaaatgcc
     4081 agcctaccag cgcatatagc gcacctacca cgggatagaa ccagtcaaga ctcatctggg
     4141 gtaaaatatc ggagcaacaa tcccggagtg tacaacaact ccaccctgtt ttggcgagcc
     4201 gcggaacatc cattcatgct ggaaaacctg actacccgac tgacctccgt catcaaaaac
     4261 ctgcgcggac aggcgcgact gaccgaaagc aacatccagg atgccttgcg cgacgtgcgc
     4321 atggcgctgc tggaagccga cgtcgccctg cccgtcgtac gcgacttcat ccagaacgtg
     4381 cgccagagcg ccctgggcac cgaagtgctg acaagcatca cacccggcca ggcattcatc
     4441 ggcgttgtcc accaggagct tacccgactg atgggggaac aggccagcga actgaacctg
     4501 gccaccaccc cacccgccgt gattctgatg gccggactgc aaggctccgg caagactacc
     4561 agcagcggca agcttgcacg gtggctgcgc gaacagcagc ggaagaaggt gctgctcgtc
     4621 agctgcgacg tctaccgtcc cgccgccatc gaccagctgc gcaccgttgc cgagcaggct
     4681 ggcgcgcaat ggtttccctc cacgcccgag cagcgtccgg tagacattgc gcttgcggcg
     4741 cgcgactatg cgcgcacgca ttatcacgac atcctgatcg tcgacactgc cggccgcctc
     4801 ggcatcgatg aagccatgat gcgcgagatc agcgatctgc acgaggccgt caaaccgatc
     4861 gaaaccctgt tcgtggtcga tgccatgcag ggccaggacg ccgtcaacac ggcacgtgcg
     4921 ttcaatgatg cgctaccgct caccggtgtc attctcacca aactcgacgg cgacgcacgt
     4981 ggcggcgcgg cactgtcggt acgccacgtc acgggaaaac ccgtaaaatt cgtcggcgtg
     5041 ggcgaaaaac ttaccgggct ggaacgcttc tacccggaac gcatggcttc gcgcattctc
     5101 ggcatgggcg atgtgctctc gctcatcgag gatgcacagc gcaacgtcga ccagaccgaa
     5161 gccgcacgcc tggccgacaa ggtgcgcaaa ggcaaggggt tcgacctcaa tgatttcaag
     5221 gcgcagatcc agcagatgcg caagatgggc gggctgtccg cactgatgga caagctgcct
     5281 ggagtatccg gtcaggcgct gcccgctggc gcggacgaaa aggctgtcaa ccgcatcgaa
     5341 ggcatcatca attccatgac cccgctcgaa cgcagcaaac ccgaactgat caaggcatcg
     5401 cgcaagcggc gcatcgccgc cggtgccggc gtgcaggtgc aggaagtcaa ccgcctgctc
     5461 aaccagttcg agcaggcgca gaaaatgatg aagatgatct ccaagggcgg tggcatcgca
     5521 aaaatgatgc gcggcatgaa aggcatgctg ccgggcatgt gacggggctg tagccatgca
     5581 cgtttacatc tattaccgcg tcagaaccga cgatccggaa accgaaaccc tgatccgcgg
     5641 cctgcaggcg cggctggcct gccgcgcctg catcaacggg cgtttgctca agcgctgcga
     5701 cgacccgctc acctggatgg aaatttatga agacgtggcc gatgcgcagg ccctgttgcg
     5761 gcaactcgat caactggcca gcgaatacga cctgatgccc tttctggacg gcgaacggca
     5821 tgtggaatgt tttgtatcac tccgcacgcc tgaacacagc ctttgccgcg actgacccat
     5881 gaaccctgcg ccggcactga gcccgcgttc caggcgcacg atttcacctg aatacccaca
     5941 tgacttctgc ctgggacgcg gtgattcatt ttccacgggt cgcgcggaat caatcaccgt
     6001 gtcccgtggc atctgtttgc gcgtgagctt gcagattgtg gtgcggtcat cgactcaatg
     6061 tctggctggg gtccgggtat gcggccatcg gcaaggtgcc ggcaaggcgg cgaggcggcg
     6121 aggcggcgag gcggcgaggt tgatcgtggg cagcagcatt ggccctcatg cgaacacgga
     6181 tatttaataa aagtgaaata tatttacatt tatagtttac gatgattaaa taaccgcatg
     6241 cctatccgtc atgccaccct gcaccagctg aagattttcg ataccgtagc ccggcgcatg
     6301 agctttgccc gcgcggcaga ggaattgaac ctcaccccac cggccctgtc catccaggtc
     6361 aagcagcttg ccgagattat cggccagccc ctgttcgaac aggtcggcaa gaaaatctac
     6421 ctgaccccgg ccggggaagc catgacgacg gcctgccgcg acatccttga ccggctggaa
     6481 tgtgtcgccc aggagttggc cgcactgcag gggctggaaa aaggcagcct caaactcgcc
     6541 attctctcca ccgccaaata cttcattccc catctgttgg cgggtttttg cgaaaaacac
     6601 gcaggtgtcg acaccgcctt gttcgtcggc aaccgggaaa cgctgctaga gcggctcgcg
     6661 cacaaccagg acgacctgta tattctgggt caactgccag agcacatgaa tgtcgtcgta
     6721 gaaccttttg ccgacaatcc cctggtcgtg gtcgttcgcg ccga
//
LOCUS       DAIANL010000066        21908 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_686757, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000066 DAIANL010000000
VERSION     DAIANL010000066.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 21908)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21908)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21908
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686757"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3764 a         6151 c         7543 g         4450 t
ORIGIN      
        1 cgcatctggc gcgccagcgg tcccgcctgt gggtccatgc caccgaaaaa caccagttgc
       61 gggtcttgcg ccttgatgct cgtcaggatg gcaagaaaat cggtggcgtg atcggtggtg
      121 tattcgcgcg ctacaatttg cgcacccgct gcgccggccg cacgggcgaa ttcgtcggcc
      181 agtccttgcc cgtaggcggt acggtcgtcg atgatggcga tgcgtcgtgc gccgagcgtt
      241 tgcacggcgt actggccgag tacgccgcct tgctgtgcgt cgttgctcat gacacggaac
      301 gccgtgcgaa aacccaggcg cgtataggcg gtggcggtgg aagatgggga tatttgcgga
      361 atgccggcgt cagaatagac cttggcagca ggaatggtgg cgccggagtt gaggtgaccc
      421 accacgccgg ccacgccgtc gtccgccagt tgctgcgcga cggtggtggc cgtttttgga
      481 tcggcctggt cgtcctcggc cagcagttgc agctggcagg gtttgccgcc gatggtcagt
      541 ccggcggcat tgatttcgtc caccgcaagc tgagcgccat tttcgttgtc tttgcccaga
      601 tgcgcctgcg ggccggtgag cggagaggcc gagccgatct tgatgaccgt tttgccgttg
      661 ctggcaggct ggccgcaacc ggacagtgcc agcgcgcaca acagcagggg aacgggccaa
      721 cgcattgccg cagtcatcag gattctccac gagtcagact gtcaggttat agcataaagt
      781 gcgccaaacc ggaaccgccg gagcagcgcg caggtggcgg cagggcaaag agggtccggt
      841 catccgcgcg atgtggcagc ggtgacaggc aggcgtatgg tcatttcccc gttgagcgcg
      901 gatttgccgt gccgcctggc tagtcctttc cggccaatgg atggccgaaa agcttctttt
      961 gggtcggaca gtcttgtaga atggtgtatt catgtgattt ttcagctttt tgtggtggca
     1021 ggccggcaga caaccgtctt ttgccctgca tacgggttta tcgttggcct tgtttcggga
     1081 gccggtcttg aatcactcca cttcgtcgtc ctatcgtccg acccggacgg ctgcgctgat
     1141 cgtcatcgcc gtactggtca tgggcatcgc ttaccgtggt gggctggccg atctcgcaca
     1201 tcgctggaat acgcaggaag agtatggcca tgcctggttc attccgctga tttcggcatg
     1261 gatgctatgg cgcaagcggc aagccatcca gccgctgctc ggccggggca gctggtttgg
     1321 accgctgctg gtgctgtgtg gtgcggccgg gttgctgctg ggcgaatata cggcgatgtt
     1381 cctgttcatc caggtcgggt tcctggtgag tctggccggg ttgacgctga gcctgggggg
     1441 gagactgctg ttgcgccagg cggccgtgcc gctgctgttc ctgctgttcg ccattccgtt
     1501 gccgtatttc cttgaggccc agctgtcgtg gcagctgcag atcctgtctt cgcggttggg
     1561 agccggggcg ttgcagctgc tgggcattcc ggtattcctc agtggcaacc tgatcgacct
     1621 gggtgattat cagctgcagg tggtcgatgc ctgtagcggt ctgcgctatc tgtacccact
     1681 catgagtatc ggtttcctga tgggttacct gtaccaggct cgctggtgga agcgcatgac
     1741 ggtgtttctt accgccattc cggtgaccgt gctgatgaac agcttccgca ttgccgtggt
     1801 cggcgtactg gtggatggct ggggcaatgg catggcggag gggttcatgc actatttcga
     1861 gggctggctg atcttcatgg cctgcctgct gattctgctg accgaaatcg ccgtgttcga
     1921 acgttttggt gcgcggcgca gtttgcatga ggcgatgcgc atgccgcaga tgccgcctcc
     1981 tgctgcgttg gttgagcacc gcctgccggc gacgttctgg gtggtgctgg cgctgctggt
     2041 agcgacgctg gtgctcgacc tgcgagtcac ctcgcgcgcg gaattcattc caccgcatcg
     2101 caatctggcg ctgtttccgc tcacgctcgg tccctggcag gcgcgtccca ccattctggg
     2161 tcagggggtg ctggatacgc tcggtacaga agattacctg atggctgatt acttcagtcc
     2221 gcaaggtatg gtgaatttct atgtctcgta ttatgcatcg cagcgcaagg gagcgtcgcc
     2281 gcattcgcca gaggtgtgca tgcccagcaa cggctgggtg atcacgtcgc tcgaacccat
     2341 ccgggtgatg ctggacggcg gcaggcgtgt cgatctcaac cgcgccgtga tcgcgcagaa
     2401 tcgtgaccgc cagctggtgt actactggtt cgacgagcgc gggcgcagca tcgccaacca
     2461 gtactggatg aaatggtacc tggtgcacga tgcgatcacc atgaaccgta ccgatggtgc
     2521 gctggtgcgg ctggtgaccc cggtgcagcc aggggagccg cttgaacggg ccgatacccg
     2581 tctgcggggt ttcatgcagc tggccttgcc caaactggtg acatacgtgc caggcaggtc
     2641 gtaggcaacg gagaagaact gcgcaggttc aggtgtcgcg gcgcaggatg aatgcggctt
     2701 ccgcgcgaga cagaccggcc gcgtcataaa aaccggcggc atccggtgcc gaaaccagga
     2761 tgagcgtggt cccggtaccg gcagccgccc gggtttccgc gatcagacgc ttgccgatgc
     2821 catgtcgctg atagtgccgg tctacggcga gatcggacag gtaacagcag tacgaaaaat
     2881 ccgtcaatgc acgggaaacg ccgacaagcc ggccatcgtc gcgtgcggtg attaccaggc
     2941 tggcgagggc gagcatgcgt tcgatgcgcg ggcggtcatt gaccgggcgt cgttcggaca
     3001 gtcccgatcg ctggagtaca tcgatgaatt ccgcaaccgc cagaccggtc tcgcgcgcat
     3061 agcagatgtt gtccatggcg ggcttctccc ggctggcggc cgtgatctgt cgtcgggcgg
     3121 tgtgcgcggt tactgcgcca gcacccactg caccagttgt tgcaggtcat gcgcgctgga
     3181 ttgcgcgttg gggggcatgg tcgtgttgcc ccagtcgccc gtgctgccat gctgcaggcg
     3241 tgcgagcagg taggcagcgg cttgcggctg gttgcgaaag cgctgcgcga tggcatgaaa
     3301 tggcgggccg atgaccttgt ggtcgatggc gtgacaggtc atgcaggcat tctcgttggc
     3361 cagtttgagg ccgggatctt ccgcagcgtg aacagcgacg ctgctggcaa gtgcgacgag
     3421 gccgaaggcg agggtcaggg tggttttcaa gcggtctccg gtacggttcg ggttgcggcg
     3481 gggatcagcc agtcgatgat gccttgccac tgttcgcgtt cgcggcgggt agcgtgcagc
     3541 ggcaggtcaa aaatgctttt gccttcgaaa gcgctgttga cgtaggcctg ggtgtcctgc
     3601 agcagggtca gcaacggcag ttcgaggcta tcaagccagt gttcgagctg gtgcgcggca
     3661 cgggtgcgcg gggcgacgcg catgccgacc atgccgatga aactacgttg tttgcggata
     3721 gccttttctt cctgcagcgc cagcagaaac tcctggctgg cggcaaggtc gaatgccgac
     3781 ggcaccacgg gaacgacgac gtgccgggcc agtttcagcg tgcgcccgag catcttgccg
     3841 tgcaggccgg ccgggctgtc gatgacgagc cagccgctgt gcccggcacc ggattgggcg
     3901 aggtcgaagc gccagatggc ggggaggtct gcgggtcgct gttcgagcca gcgctgtgcc
     3961 gaatgctgcc ggtcgaggtc gagcaggcgc acgttttcgc cgcgtgccgc aagccaggcg
     4021 gccagattga ccgccagcgt ggtcttgcca ctgccccctt tcgggttggc gaccagcagg
     4081 gtgttcatca tgtgcgtggt tcgatctggc tcacgtcacg tacggcaccg gttgcggctg
     4141 aggtggtgag cgcggcgtaa gcacgcagcg cagcggacac ctggcgctgg cgctgttccg
     4201 ggcgccaggc gcgagcaccg cgagcgagca tggccgtacg gcgggcttcc agtgtggcct
     4261 cgtcgacagc caggtgaatg cggcgcgccg ggatgtcgat ctcgatgcgg tcgccggctt
     4321 ccaccagcgc aatcaacccg ccttcagcgg cttcgggaga gacgtggccg atgctgaggc
     4381 ccgaggtgcc accggaaaag cggccgtcgg tgatcagggc gcacgatttg ccgagacctt
     4441 ttgacttgag gtaggcggtc gggtagagca tttcctgcat gcccggaccg cctttggggc
     4501 cttcgtagcg gatcagcacg atgtcgcccg gctggatgac atcgccgaga atggcttcga
     4561 cggcactgtc ctgcgattcg aaaatgcgtg ccgggccggc aaagcggaac agggcttcgt
     4621 cgaccccggc ggtcttgacg atgcacccgt cgcgcgcgac gttgccgtac agcaccgcca
     4681 gtccaccgtc ccgggtgtag gcgtgagcca gatcgcggat gcagccgtgc tcgcggtcgg
     4741 tgtcgttgtc cggccagcgc gaatcctggc tgaaggccgt ctgggtggga atgccgcccg
     4801 ggccggcacg gtagaaggtg cgaacggcct cgtcgtgcgt gcggcggagg tcccactggt
     4861 cgatcgccgc accgagcgtg gtactgtgca cggtgggcag gtcgcggtgg acgagacctg
     4921 cgcggtcgag ttcgccgagg atcgccatga caccgccggc gcgatgcacg tcttccatgt
     4981 ggtagagcgg ggtggccggg gcgaccttgc acaggttggg cacccgccgg ctcatgcgat
     5041 cgatgtcctg catggtgaat ggcacaccgg cctcgcttgc ggcggccagc aggtgcaata
     5101 ccgtgttggt cgatccgccc atggcgatgt cgagcgcaat ggcgttttcg aaagcggcaa
     5161 atccggcgat gctgcgcggc agcgcgctgg tgtcgtcgtg ttcgtaatag cggcgggcca
     5221 ggtcgacgat gcgtcggccg gcctcaagga aaagctgctt gcggtcggca tgggtggcga
     5281 gcatcgagcc gttgcccggc aggctcaggc cgagggcctc ggtcaggcag ttcatcgagt
     5341 tggcggtgaa catgccggag caggagccgc aggtggggca agccgaacgc tcgacttcgg
     5401 ccacggtggc gtctggttca tgtgcgttgg cagcgagaac catggcatcg accagatcga
     5461 gcttgaccgc cttgccgtgc agtgtggcct tgccggattc catgggtccg cccgagacga
     5521 agatgaccgg aatgttcagg cgcagtgctg ccatcagcat gcccggggtg atcttgtcgc
     5581 agttggacag acagaccagg gcgtcggcgc agtgggcgtt gaccatgtat tccacgctgt
     5641 cggcaatgag gtcgcgcgag ggcaacgaat acagcatgcc gtcgtggccc atggcgatgc
     5701 cgtcgtcgat ggcgatggtg ttgaattcct tggctacgcc gccggcggcc tcgatctcgc
     5761 gcgcgatgag ctggccgaga tccttgagat gtacgtggcc aggcacgaac tgggtgaacg
     5821 agttggcaat ggcgatgatc ggtttgtcga aatcggcatc ggtcattccg gtggcgcgcc
     5881 acagtgcgcg ggcgccggcc atgttgcggc cctgtgtggt ggtgcgggat cggtaggcag
     5941 gcatgtgttc ttcaccttct ttgatgcgat aatgctggct attttaaccc gaatcgcctc
     6001 cgacatgctt gttcatcccc aattcgatcc ggtcgccatt tcgcttggac cgctgtccgt
     6061 acgctggtac ggcctcatgt atctgctggc attcgtgctg gtgctgctgt tgggtcgctg
     6121 gcgcattcgt caccaccccg aatccggctg gcagatgcgc cagctcgacg acgtgctgac
     6181 atggggtgtg ctgggcgtag tgcttggcgg tcggctcggt tatgtgctgt tctacaagcc
     6241 gggtttttat tttgcgcatc cgctgcaggt cttttctgtc tggcagggcg gcatgtcgtt
     6301 tcatggcggc tttctcggtg tgctgttcgc attgtggtgg tttgcccggc gcgaaggcaa
     6361 gtcctggctt gcggtgacgg atttcatcgc accgctggtg ccgcttggac tcggcgcggg
     6421 ccgtataggc aatttcatca atggcgagtt gtgggggcgg ccgaccagcg tgccctgggc
     6481 catggtgttt ccccaggtcg acctgcagcc gcgccatccg tcgcagttgt atgagttcgg
     6541 gctggaaggg ctgatgctgt ttaccatcct gtggtggttt tctgcgcgcc ctcgtccggt
     6601 gggggccgtg tcggcgctgt tcctgatcgg gtacggctgt ttccgcttcc tggtcgaatt
     6661 cacgcgggaa ccggacgatt tccttggatt gctcacattg ggtctcagca tggggcaatg
     6721 gctgtcgttg ccgatgatcg tcgccggggt gctgatgatg gtgtgggcct accggaggca
     6781 gggcacggca tgaccctgac cgagctgcga tacatcgtgg cggtggcgcg tgagcggcat
     6841 tttggccggg cggccgaatc gtgccatgtg tcgcagccga cgctgagcgt ggcgattcgc
     6901 aagctggaag acgaactggg agtgacactg ttcgaacgcg cgatcaatga ggtcagcctg
     6961 accccggccg gaacgcgcat cgtcgagcag gccgctcgcg tgctcgaaga agcgcagacg
     7021 ctgaaacaga tggcgcggca gtcggagaat gcgctgtcgg gcccgctgcg gctcggggcg
     7081 atctataccg ttgcgcccta cctgttgccg gcgctgattc cggcgctgca tgagctggca
     7141 ccgcacatgc cgctgctgct ggaggagaat tttacctatc ggttggcgga acgcctgcgt
     7201 cagggagagc tcgacgtcat cctggtggcg ttaccattcg acgagccggg cgtggtggtg
     7261 cgtgagctgt accgtgagcc gttccgggtg ctgttgcccg aagcgcatcc ctggcgggag
     7321 cgcagccaca tcgctgccga tgagctgcgt ttgcaggaca tgctgctgct gggtgttggc
     7381 cactgttttc gcgaccaggt acttgaggcg tgtccggcac tgcagcgcgg cgacgcaatc
     7441 gccacccggc agacgctgca gtccggtagc ctggaaacca tccgttacat ggttgcttcg
     7501 ggtgccggca tctcggtcat gccctgcacc gccatgcaac agcacagtct tcccgggctg
     7561 atcgatcggc cgttcgcagc accggagccg caccgcagcg tggcgatggc gtggcgcagg
     7621 agctttcctc gcgctgcggc gctgcaggtg ttgcagcgcg ccatcgttgg cctgaatctg
     7681 ccctgcgttc agccggtgtc ctgaagtggt ttcagtgttt cgggcctgtg ggtacgggcg
     7741 gcagaaagaa ctcgcgtgga tgggtgacca gtcgccgcag cacgctgacc gcaagcagcc
     7801 tccattgggc aaatgcgatc tgtcgtgcgc gtagcgcaac cgtcagtgcc agcgcaaagc
     7861 tgacagcaag attggtcaaa ccgatggcag tgatgccggc ggccgtccat gcgacgatgt
     7921 tccagtcagc gtggaagttg aaggcggcca gcgaatagcc ggcataggcg gaggagaagg
     7981 cgatgtggcg gatgtcgagc ggcaggccgg acagggtgcc cagtgccgtg acgccgccga
     8041 gcatgcagcc gaaccagaag ttgcctgcca gcgcgcccag gttgttttcc acatagcgta
     8101 ccagccgctg ctggcgccgt tggcccagca gccgtcgtag ccagccgagt tgcatgagcc
     8161 gctgcggaat gttgttgtaa gcgcacaaat tgtcatagta gcctgcgatc agaccggaga
     8221 ggaacagaca ggcgccggcg atggccgcgt aggcgatggc cgcgctgtgc agtgggtgga
     8281 tgccgtctac cagatgccgc gcggtgtcca tgctgatgac cgggctgttg ccaaagtgct
     8341 gtacggcaaa gccgatggcg gccgagatcg gcaccgccat cagcacgttg cccaggatag
     8401 cggcgaactg gctgcgcgtg gtgcgtgcga tcatcgtcgt caggccgtcc aggtctggtg
     8461 cctgtccgtt gttttcgccc aggcttgcgg ccagggtggc ggccgtcatc gccggttgct
     8521 tggtggctac ggtgaaacgc aggacatgga ttatcatgaa gccgaaaccg tagttcaggc
     8581 agatcaacag tgcctggctc aatggcggca ggacctgttc gccaatgttc agcttgagca
     8641 gggccatgag cgcgatgatg acaccggccc ccagcgccga gcgccacatc tgggcatact
     8701 ctgcccggtt ttccgcgatg tagtgctcgc cagcgcggct ggcattttcg gtgacgcgca
     8761 gtgccagcag gcccagactc tgattccagt ggtggcgaag atcgtttttg cggcattcgc
     8821 cctgcaccag cactttgaac aggctgactt cggggtgatg gttgctgccg tggccggtgt
     8881 gctgttgccg cagcgagacc aggcaggcgt acagggctgc gttgcgttcc agatgctgtt
     8941 gcagtcgttg cagcaggtag gtgaggcgaa tgctggtgcc ctcatgcgtg gcgcgatggt
     9001 ggatgcggcg gatcaacagc gtgccctggt tcagcagccc gacaacctgc gcgtcttcgt
     9061 taccatcggc atcttgtcct gcccacgcgg attcgtagcg ttcgagccag tccagggtcg
     9121 cctgatgctg ggcaagaaat ggagagatgc cctgttcgat ttcgggctcc agccgcagca
     9181 gttccggttc caggccgatg gcggcgatat ggaacgacag tatccgtacc gagcgggtaa
     9241 tctgcgcgat ggcgggtggc aactccgtgc tgcgaggcac ttcgtcgaaa cgcagcgcct
     9301 gcagcagttc gatccagcag ctgtccggca ccgcattgac ccagcattcg tcacccgggc
     9361 ggtggaaaaa ctgcgacagc aggtctttca ggcggaaggc atcgtgtgcg gcgggcagca
     9421 gcttgtgtga gatgcgccgt gcggcttcgg aaaagaaccc ggtgttcggc aggattccgg
     9481 tcgaagtgta cagcgaaatc tgctcgcgct cattgagcag cgccagcagg ctggtacgca
     9541 attcgacacg caggacgggc tggcgagaca ggcgataaca cagcgcgcag atggcctgta
     9601 cggcagctgc ggcatcgtgt ggccgggccg gacgaagcgc atcgatgacg ccagccaggc
     9661 gggcgacgtc gtcctttcca gtcgggttgg cggagtctga cagcaatttc ataaggcaga
     9721 tcgacctgtg gcagccaagt gtacagtaac ccggagtcat atggagtgcc cggaaggcga
     9781 aatggttcac cgccagcgtt gcgatgggtc ggcatggacc ggcaatagct ttattcaatc
     9841 gacagtattg caacaatcaa ttggatgaat gcgaacgggt tgtatacgat gtaaaccatg
     9901 agcagacggc tcagaaaccc aacaggagaa cgatcatgag cgtgaccatt cagaatctgg
     9961 aagcggcttt tgccggcgag tccatggccc atatcaagta tatgtatttt gcgcggctgt
    10021 gccgggcgct cggcgacgag acgacggcca gggtgtttga agaaaccgca gcacaggagg
    10081 tgatgcacgc ctttggccat ctcgatttgc tgtatccgca gcaggacatg tcggtggcgc
    10141 gctgcctgca gatggcgatc gaaggggaga cctacgagta caccgaaatg tacccttcgt
    10201 tccgccatgc ggcaatggaa gaacgccagc acgaggcggt ggcggaaatc gacagccaga
    10261 tcacggaatc gaaggaacat gccaaccgct tccgcgcaac actggacaag gcggcgaaac
    10321 gcttcgccgc actggccagg gtggaggaga aacatgcgcg gcattaccag cagacactcg
    10381 atgccgtgca ggcagcctga gcgaaacatt gaaccctttg aaaggaccga agtcatgaag
    10441 aaataccagt gtgtcgtgtg tggtttcatt tacgatgagg cgttgggttt gcctgaagaa
    10501 ggcatcgctc cgggaacctc ctgggcggac attcctgaag actggagttg ccccgactgc
    10561 ggcgtggcca aggccgactt cgagatggtc gttctgtgaa tgcgaaaggg ctgcccgtca
    10621 gggcggcagc ccgattgagc gagctggagg aaattgcatg catccgatgg tgatcgttgg
    10681 cagcggaatg gcgggttata cccttgcccg cgagctgcgc aagcgcgatg ccgccgtgcc
    10741 gttgctcgtg cttactgcgg ataacggcga tgtttacagc aagccgatgc tgtcgacggc
    10801 gctggcacag gggcagaccc ccgaacgact ggtgcagatg acggcggcgc agttgcaggc
    10861 gcaactcggg atcagcgtac gcaccatgac acgggtggag tcgatcgatg cgacggcgca
    10921 taccctgtat gccgggggtg agctcattgc ctggagccgg ctggtgctgg cgctcggtgc
    10981 caatccggtc gacccgcatc ttgcaggtga cgcagccgat acggtgttca gcgtgaacaa
    11041 cctggcggat tacgcgcgtt ttcgcgcggc gcttccgtcc ggcggcaggg tggtcatact
    11101 cggcggtggg ctgattggct gcgaattcgc caatgatctc gccgcagcgg gacatgccgt
    11161 cgaactggtg cacccgcgcg agtggttgct ggaacgcctg ctgcccgaac aggccgggcg
    11221 ggctgtgcag gccgcgctgg gtgggttggg cgtgcagagc catctggggc gcaaggccac
    11281 ggcggtgcaa cgggcggacc atggggtcac ggtcgtgctg gatgacggac gccgtctgca
    11341 ggccgatatc gtgctgtcgg ccattggtct gcgtcctgcg actgcattgg cacagcaggc
    11401 cgggttggct gtgctgcgcg gtattgtggt ggatcgctgg ttgcgcagca gtgcagcgga
    11461 tatttatgcc atcggtgatt gtgccgaagt ggatggatat gtgctgccct acgtgcagcc
    11521 attactggca caggcacggg cgttggcggc catcctgtgc ggcggcgatg cgccgttggc
    11581 atacccggtg atgccggtgg tggtcaagac acccgcgtgt ccggtggtga gtctggcggc
    11641 cgccgacggg cgggcgcttg actggcaggt cgagcaggcg gaggacggat tgcgtgcgcg
    11701 tcagatcgat gcgacggggc agatggcggg attcgtcctg accggtgcgg cgacgcggga
    11761 acgtcagcag ttcgccgcgc agctcaggcc gttgttggcg ccggccagct gatggccggg
    11821 gcgcagccgg gtcaggcctt gttggcctgt tcttcggcaa tttccttgtg cacttgcgcc
    11881 atgtccagtt cgcgcgcctt gccgatcagt tcatcgagcg ccgaggcggg caacgaaccc
    11941 ggttgcgaga agatgatgac tttttcacgg aaaatcatca gcgtgggaat ggagcggatc
    12001 tggaaactcg cggccagcgc ctgttcttct tccgtattga ccttggcgaa taccagatca
    12061 gggtactttt ccgacgcggc ggcaaatatc ggcgcgaagg aacggcacgg gccgcaccat
    12121 ggggcccaga agtcgatcag gatgaagtcg ttgttgagaa cggtggattc gaaattgtct
    12181 tgcgtgagtt ccagtacggc cattgtccgg ctccttgctt gggtttcaga caacacatta
    12241 acatgccggc ccgaggttgt cagcacgggc aaggaccgca atccgacccg gtggttgcac
    12301 cgctgtgaca ttttgtcgcg cgtttgcgct gcgtgcgagg ctgtgtagcg ccgcccgcgg
    12361 cgaccgacgc ctgctagaat gggcgcatga tccgcctgtt ttccgtttct gccctggcac
    12421 ctgccgaggg cgtgctgcgc gaactgctgc tgctgcgcgg ctatggcctg ctggcccaac
    12481 tggccgtgct ggcggtagcg acgtgggtac tgcgcatcga actgccgttg cacgcattgc
    12541 tggccgtggt ggcaatgacg gcgatattgg cagtgctgac cgggctgcgc ctgcgccagg
    12601 actggccggt cacggcgctc gaatgcctgc tgcagctgct ggcggacgtg gcgctgctga
    12661 cggcgttgct gtatttttcc ggaggtgcgg gcaatccgtt cgtgtcgctg tacctgctgc
    12721 cgatcatgat tgcggcgacc accttgccgg tacggcttgc ctggctgctg actggcgcct
    12781 gcctgctggc ctacagtctg ttgatgcggg tgtatgagcc gctgaccctg ccaccgggcg
    12841 catcggcttt cagcttgcac ctgatgggca tgtggctcaa tttcattctc agcgcgctgt
    12901 tgatcaccta tttcattggc cgcatggcgc aatcgttgcg caaacgcgat caggccctgg
    12961 cacagatccg tgagcggcgc atgcgcgacg aacagatcgt tgccctgggc aaccttgcag
    13021 caggcacggc gcatgaactg tcgacgccgc tggcgaccat gagcgtgctg gttggcgagc
    13081 tggccgaaac ctgtgctgac cggccagatc aggtggccgc agtggagatc ctgcggcagc
    13141 agatccgtgc cagcaaagat atcctgaccc ggctggcgcg ccgggatagc gcggggcgcg
    13201 ccgaatcggg cggcgtgatc cggcttgatg tctggctgca tgggctgctc gaaacctggc
    13261 gtgtggtgcg cccccaggca caggtcagtg tgcactggct gcgtgatgcg cctgtgccgg
    13321 agctgattcg gggcgatacc ctcgatcagg ccgtgatcaa cctgtgcaac aatgctgccg
    13381 atgctggtgc gggtcaggtg cagatcgagc tggactggga caggcaggcc attgaactgg
    13441 cgattctcga ccggggtagc ggttttcgca gcgaattgcc cgcagggacc gcgattttca
    13501 gcaccaaacg tgagcatggc ggcattggca tcggtttgat gttggccaat gccagcgtgg
    13561 aacagtacgg cggcagtgtg agcctgctag accggccggg cggcggtgcc cgggtggtgg
    13621 tgcgtctgcc gcggcacgga ggagcgctgc atgaatgaaa cgtcattgtt gctggtggat
    13681 gatgacccgg tgtttgccgg ggttctggcg cgggcgctgg gacgtcgtgg ttttgcggta
    13741 cacgaggcgc atggtgtgtc acaggcgctg gcattggcac gcgcggcgcc gccggcctat
    13801 gctgtggtcg acctcaagat gtccggcgaa aacggtctgg tgctgatccc ggcgttgcgg
    13861 gcgttgcgtg ccgacatgcg tattctcatg ctgacgggct atgccagcat cgccaacgca
    13921 gtggaggcga tcaagctggg cgcgacgcat tacctggcca aaccggcgga agtggacgac
    13981 atcgttgctg cgttgcaccg cgatgccggc gatccggcgt tgaagctgcc ggtacggccg
    14041 tcatcgctgg aacggcttga atgggagcac attcaacgtg tgctcaatgc gcacgacggt
    14101 aatatttctg ccacggcacg ggcgctcaac atgcatcggc gcacgctgca gcgcaagctg
    14161 ttcaagcgac ccgtgcgcga gacctcctga gcactgcggc tgtcacgatc agccgcttgc
    14221 ggaaagcaga ctgtgctgtt tcagccagtc gaggaaatcg tgttcggcga gggggcggct
    14281 gtacagataa ccctggaaat gcgtgcagcc aaaacggagc agtgcatcgc gacaggttgt
    14341 gttttccacc ccctccgcca cggtcgacag gtgcaggcgt tgaccgatgt cgatgatcga
    14401 ggccaccaac gggttgtccg gctgagtctc aagtttgtca acgaatgact tgtcgatctt
    14461 gatcgcatcc agcggcaatt gttgcaggta ggccagcgat gaatatccgg tgccgaaatc
    14521 gtcgagcgca atgcggaatc cggatttgcg taacagatcc agtttgctta tggttgcggg
    14581 caggtcgtgc aacaaggcgg attcggtgag ttccagcatg atgcgctgtc gcgggacccg
    14641 gtgttcgctg gcggcgcgca tcaaccggtg cacgaaatcc gcgctggcaa actgccaggg
    14701 gctgacattg atcgacaggt agccgtgaaa gctggtccag tgcgggaggc gggaaaacgc
    14761 ctcggtgaac agccagtcgc cgatgctgtg gattatgccg gtttcttcag cgatcggaat
    14821 gaactgcccg ggcggaatca cgccatgcag tggatgaatc cagcgaagca gcacttctgc
    14881 gccgaccacc tcgccgaggt catcgacctg gggttgaaaa tgcagggcca gttcgttgcg
    14941 ctccagcgcg tttcgcagac cggcctccag ttgcagccgc ccggttgccc tgacttgcag
    15001 tgtggtctca tacaggctga tctggttgcg cccctgtttt ttggcagcat agagcgccat
    15061 ttccgcatgg cgcagaacat cggcggcttc gtcctgcgct tccggaaaca aggccatacc
    15121 cacactggcg cctacccgca gcgagtgctc atgcagactg acggatgccg tcagggtgtc
    15181 atgcaccgtt ttcgccattt cgtcgacata ttgcgtgaac cggcttgcag aaagtgtgga
    15241 tgccggaagg atgatggcga attcgtctcc gccggggcgg gcgatgaagg cctgattgaa
    15301 ttgttcagtc agccgttgcg ccaggcgttg caggatcatg tcgccggtcc ggtgtcccag
    15361 tgcatcgttg atggttttga aatgatccag atcgatcagt aacagtgcgc cccgctccag
    15421 gcgtgctctg gcggcgcaga tgtccgcttc cagccgctcg tgaagacgga tacggttggg
    15481 caaaccggtc aacaggtctt cgtaggccag tcgttgcaag gtgtgctgca cgagcatctt
    15541 gtcggtcacg tcctcgtgga ccaggactga cgcctgaatg gctccggatt cattgcggat
    15601 cgggtaagca tagccgcgta tccagcggat cgcggacgcg tctgtgtcct gttcattccg
    15661 ccgggtgatg gcgatggcgg gaatttcgct cacggtgccg tgcatgttca ggcgcaaggc
    15721 ttcctgcagt ccatcgatgc tgcagtcggg atcgtggaat atgttgcagc gcgccccggg
    15781 cggaatggtc cgttcccaca gtcgggtcca ggcgggattg gtctgcagca cagtgccgtc
    15841 cggcgcgagc accatggtgc tgaatggcga ttgctcgatc aggctgcgga agcggctttc
    15901 cgactcacgc aggcggtcat gcgtctgttg cgccagcacg aggctcagca tgaggatcat
    15961 gatcatgaaa ctgaacatgc cgagcggaac gaacggaatg atctgcagcc ggccgagtat
    16021 cccgatagtg gagccggaca gaaacagggc gtaactgacc agcatgccca gtgtgtaccg
    16081 gtccggatgg gcatgatgat gtcccgccag ccggcgcaga ccgtcgagca ggatagtgct
    16141 gccggcgaag atggagaaat aggaccagtc gctgctgagt cccgtacctg tgatgacgat
    16201 gtcgccccag ggcagggtct gcgcatggat accggtgaat accctgtatt gcagggtatg
    16261 gggcagaacc aggttggcga gcagcaggcc gacggcaacg gtgttgagta gcaggttgag
    16321 gcgaagggtg ttctggttgg tataagccga aagaaaatgc ggcaaggcga tcagcatgag
    16381 gatgatgaag ctcagattgc cgcgcaaggc ggcaagatac gaggcggcat cgggggcgcc
    16441 gatggcgcgc aggtaaaaaa atccgcgggc acggcagcaa gcagcatcag accaaacaac
    16501 acattgaccg gggcatgggg aggtttcaga ccgaaagaca gatgggtggc ggcggcatac
    16561 agcgtaaagc cgatcaacac gtagttggca gaaacgatat ggctgtgcat ggcgcctcct
    16621 ctcggtgtgg tcacaggtgc gtgcgtctgc cttgcgttct ggtgacccgt cgccggagag
    16681 cgccggcacg gcgcgacggt tgctgcgatc tccgggttgt atcgaatgcc atcacattgc
    16741 agtctacccg ttctgctcag cgtcgtgtca ggtacaggca gagaggggcg ggcggccgag
    16801 ctgttcggcg ggtagcgggc gaccgaacag gaatccctga taatgaagca gtccgctggc
    16861 gtcgagcagg cgtttttgtt cgtcggtttc cacgccttcg gcaacaacat caagttcgag
    16921 ctgacctgcc atggccatga tggtgcgcac tacggacagg ttgttgtcgc agcggccgag
    16981 gttgcgcaca aatgacaggt cgatcttgag ttgatggagc ggcagccggc acaggtgttg
    17041 cagtgacgaa cagccggtgc cgaaattgtc gagcgagaag cgtatgccga tatcgccgag
    17101 cgctttcatg gtggcgatgg tctgttcgat gtcgtcgagc agcaggtgtt cggtgagttc
    17161 gatgcgcagg cggtcaggac ggatgccgct ggttgcggca acctcgctga ccatggcgac
    17221 aaaatccggt tgccggaact ggcgtgggct gacgttgacc gcgatgaaca ggtgtcgcag
    17281 cgccggattg gctccccatt gcgccagctg cctgcaggcg tgttcgagca cccagcggcc
    17341 gatgggcagg atcagcccgg attcttccgc tagcgggatg aattgccgcg gcagcagttc
    17401 accgagctgt ggatgctgcc agcggatcag tgcttccgcg cccacggcct gccggttgtg
    17461 gtcgtactgg aactggtagt gcaggcggaa ctgatcctgc ccgacagcga ggttcaggtc
    17521 ggcctcgagg ccggcgcgtg cgtgcagctg gtgctgcatg tgcggttcga agaagcgcag
    17581 ggtgttgcgg ccgtcccgtt tgtcctggta catggcgatg tcagtctggt gcagcagttc
    17641 gccgctggag tgcgtgttgc tgtcaaacag ggtgatgccg atgcttggcg tcaccaggta
    17701 ctcgtgcgtg gcgagctggt agggacgatt gagttcggca atgatcttgt ggccgatgat
    17761 ttcgctctgg ctgcgcgcgt cgtcgcccag gttgcccagg atgatgacga attcgtcacc
    17821 gccgatgcgt gcgacggtgt ccgtttcgcg tacacagtcc cgcaagcgtt cagccacctg
    17881 cctgagcagc atgtcgccat tcccgtgacc gagcgtgtcg ttgagtgtct tgaaattgtc
    17941 caggtcgatg aacagcagtg cgcgtgtgtg ctcgtcctgt gaagactggt gaatctcatc
    18001 gtgcaggcgg ttgagcagca ggcgccggtt gggcagtcgt gtgagcgggt cgctggtcgc
    18061 cagcgcgttg agccgggcat tcatgtgttc gagctgcagg ttctgttcaa ccagcttgcg
    18121 gttggcgcgg tccaggtcat cctcgcggca cttgcgttcg tggatgttga aggtgaaccc
    18181 gatgaagcgt cggcctggct ggtcggcagt ccggtcgacg atcctgccgt aattggcata
    18241 ccagatccag tgccccgagc gggcgcgcag gcggaattcg cagcggtagc attcgctcag
    18301 gccgtcgaga tgggactgta ctgcctggcg cagccggtct gtatcgtcgg ggtggacgag
    18361 cgagtagatg tcgtcgatgt tatgcggcgc gtcttcggca gagtaaccca gttcggccag
    18421 cgttctgccc ggcttgcagg gaatttcacc gctgatcagg tcgttttccc acaggtcgag
    18481 cccggtggct tccagagcca gttcgagccg ctctgcgtcg aggttgcgtg cggtcatggc
    18541 gtgacgggct ggtgcggcag gtcgcaagcg cggtcgtcgg tgcgacaggc agggtcgggc
    18601 gttcgtgacc gtcccgaccg gctctggtca tgggtacgga agcggccata gggaaaagcg
    18661 cttgcgccgc caatcgccag gtccattggg gggtctccgt gtaggcgtgg ttcgtcatgc
    18721 accacgcatt ctgttgattt gttctgatta taacatcaag cagaacgtca gcttgaattc
    18781 cttcaggtcc gtgttgttgc atttattaac aacagatatt atcattaaca aatgaagcgc
    18841 aatgtcaaac tccggacaat gaccttgccg gtgcggcgat ggctggcagc cgtgagtctt
    18901 gccgtcgcgg gcgcatcggc ccatgccgat tgctggagtg acgccgaatc acgctacgcc
    18961 gtgtcgcggt tcttgctgct ggcgattgcg cagaacgagt ccgggctcaa tccgtcgagc
    19021 gtgcaccgca atgccgatgg cacggtcgac atcgggctga tgcagatcaa ttccgcctgg
    19081 ctgccggtgt tgggtcgcta tggcattggt ccgggacagt tgcgcgatcc ctgcaccaac
    19141 ctgcaggtcg gggcgtggat ccttgccaat gattttgccg tgtacggccg gaactggcgg
    19201 gcggtgggag catacaacgc aaagaatgaa cggctgcgca tccgttatgc gagacgggtg
    19261 gcgcaacggt tcaggagcat agcgcatgaa tatggaatca gtcgctgaag acgcgcgcgg
    19321 cagcggcaag ggccagaagg cgttcgaggt gttgcgcggc gctctgcgcc ccattctggg
    19381 ttatctcgat gctccatcgg tgcaggaaat catgatcaat gcgccggaag agatctggat
    19441 cgagtccgaa gggatcatgc gcaggatcag cgagcgcctg cagccgatcg ccgtggaact
    19501 cgcagtacgc gcgctggcaa caagcaacga caagcggcac acaccgatca tggatgcccg
    19561 actgccgggt atccgcgtgg ccgcagtgat gtatccggtt gcaatcaacg ggcatgcggt
    19621 cagtatccgc aagcatgccg cccgcacgtt gtcgctcgac gattacgtga catccggtgc
    19681 gttttcgccc cgggcccgca gcgcgacgtt cgctgctgcg ggcgatgcgc ctgcgtcgaa
    19741 gcaggcgcgg cccgacgcga ccgcagtgcg tcagggcggg agcgcgttgc gtgatttttt
    19801 tgtctgggcg gtggcgcgac gcaagaacct gctggtggcg ggcggcacca gttctggcaa
    19861 gaccacgctg atcaatgccc tgcttgatgc cgtgccgccc ggtcagcgcg tgatcacgat
    19921 tgaggacacg gccgagctgc gactggcatc gcccaaccgg gtcgcattcg aagccaatcg
    19981 cgaactcgat gcggatattc gcgcgctggt gcgggtgtgc ctgcgctttc gacccgaccg
    20041 catcgtggtc ggcgagatcc ggggtggcga agcctatgat ctggtcgatt cgctcaatac
    20101 cggccactcg ggcggcatca ccagcattca cgccgatacc gccgaatcgg cgttcgacag
    20161 attggagacg ctgatgcgca tggcacccga tgcggcaggt ttgccgcacg acgcgctgcg
    20221 acggcgtatc gcgcagacgt tttcctttgt ggtgcagacg gcacatctgg gcggagatcg
    20281 cggcccggta cgggtggtgg aagtgcttgg atatgacaac ggtgcatacc gtcttgaaga
    20341 agttttttca cgcttgaacg gagaatagcg atgttttcaa ctgccccggg tggtgtccca
    20401 ggtttgctgt accagccgtt ctgcttcatt ctcaacaatt tcttcgacag tccggccatg
    20461 atcggcctca ttgtcgccat caccctggcc gtgggtatca tcggctggct caccgacctc
    20521 ggcggacgcg ggttcccggt gatgacgttt ctggtgatcg ccatcggggc aggcatgctg
    20581 ctcggcatcc cggcaatcat gagcggcatg ggcatgcagc tcccctgcgg gaattgacat
    20641 gcggcccaac gtgcgtgaaa cggctgtgcc ggtgcggctg tccgaggtca ggctgcttgc
    20701 cggaggcgaa ttcaaactgg tgatggccaa ctttgccttt ggtgtggtgg ttgtggtcat
    20761 gatgcacgtc tgggaatacc tgctggtagc cgcagtgctg catcttgcgc tgatgatcgt
    20821 ggcccgcagc gacgcgcgag cacgggagat ttatctcgaa tacgcgcggc agggcgatcg
    20881 ctacgttccc tggcccgatc cgcaacggca acgacgcaac aggcgcccgg cagggtttgc
    20941 gcgcagggac ctgcaatgag cgaaacgcgc gaagatgtgc gtccgtttgc cgagttgctg
    21001 ccgtggatgt tcaccagcgg cgcggatgac gtggtggtca acaaggatgg cagcctgacg
    21061 gtgatgctgc atctggccgg tatcgacatc gatgcagcca cttccgccga cgtggcctgg
    21121 tacctgcagg cactcgacgg agcctacgaa agttttcgcg aagcgccctg cgtgctgaac
    21181 tggctggtgc atcgtcgtcg gctgcgcttc taccccgaag cggaatttcc cgatccgtgg
    21241 tcggaacgta tcgaccggct gcgcggcagc cgcattgcat cggggcgcat gtacctgaac
    21301 gaagcctacc tgggcatcac gctgctggcg gataaggggg tgagcggtat cggtgcgcgc
    21361 atcgccatgc agctcgcaga aggtcgcggc gttgccggag cagtgtatta cgggctgaaa
    21421 tcggtgttgc gggccgagga tgcctttgcc tggtcagcca ccgagctgat ccagcgcatc
    21481 agtgaattcg agacgctgat tcagagattc tgcgaccggc ttgtcgggct gggcgtccgc
    21541 cgtctgcgtg gcgacacgtt acgcggtctg ttgcgccgat gcgtcaatcc aggctcaagc
    21601 cagcgcgcgg tggtggccat gccgtttctc gacagccaga tcgccgatgc ggagctggca
    21661 ttcttgcgtg acggactggt gctcgacgac cggttgtggc tgcgtggcgt ttcgctgcgc
    21721 gaaccgccac gcgcgacccg tcagggggtg tttgatgcgc tgctcgccgt cgatggcgaa
    21781 atggtgttca gtcaggtgtt ccgcattgcc ggcaagaagg agatcgctga ctacctgacc
    21841 ggcgtgcgcc aatacaacga tctcatgaaa tactcgccca cgacctggct gtatctgtcg
    21901 atgaaccg
//
LOCUS       DAIANL010000067        11745 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1102099, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000067 DAIANL010000000
VERSION     DAIANL010000067.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102099"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2236 a         3851 c         3297 g         2361 t
ORIGIN      
        1 gggagcagat tttctatggc gaattcgatg gccggcgacg caaacgggtg ctggtgaaga
       61 tcatcggcga atgaccagaa acaggaagcg cacgccgcat cgcggccgaa ttaccgcctc
      121 ttgtaggaaa aactccgtat aacaaaatat cggttatgga tactgagttt gtcgttattc
      181 tgtcgtatca tgccacttgc agacgatcca tctcacccgg aacgatcagg atgcagaaac
      241 agccagccgt taaaacgacg cggacataaa aaaagatacg caacgaggag gagcacgcat
      301 tgaaagctgg tctgctctgg ccatttctgg cctgtctgct gttaccgctt gctgcggcat
      361 ggtttgcctg gccggatacc catctgcctc ccgggttcgg tatcttccca cccgcgcagg
      421 tgcccggcgt cccggggttc aacctgctcg tattctgcgt gctggcgaca ctctgcgcgg
      481 gcattgcggc actgatcctg tttccgcgct ggttcggttt ccgccctgcc acgcccgagc
      541 cgcccgcacc gcgtgtggcg cttccgttct ggttctgggt cggaaccgtg ctcacgctgt
      601 tcttctggtg gctcatgtgg agccgtgtca ccctgttcgg caccctggtg tactacgcct
      661 tcactccgct gtggtgggga ttcatcctga tgctggacgg cctggtctac caccgcgaca
      721 acggccgctc gctactggcc tcccggccac gcaccttcgt catcagcgca ctggtctcgg
      781 tggccggctg gtgcttcttc gaatatttca actatttcgt gctgtccaac tggtattacc
      841 cgtatggcca catggcggcg ctgccacatg ccaccatcgt cgtgctgttc atgctggcct
      901 acactacggt gtggcctgcc gtgctggaat gggtcgccct gctgtacacc tttccccgtt
      961 tcgtggcacg ctaccggctc ggcccggcca ttgtcccacc cgccagcctg atgctgtggg
     1021 gcggtctcgc gctgatcgtg ctgacggtat ttttccccta cccgctattc tgggccatgt
     1081 gggtcggccc gctcagcgtg ctcaccggcc agttgctgcg caagaacatc tggaccccac
     1141 tgacgccgat gcggcagggc gactggagcg ccgtcatgat tgcagcgctg gcatcgctgg
     1201 gcaacgggct gttctgggaa ttctggaacc acggcagcga atcccccaac ccgttctcct
     1261 ggatcgtgcc gccgttcccg accaatccga atttctggat ctacgacgtt ccctatgtca
     1321 atgtcataca tctgtattcc gaaatgccac tgctcggcta cttcggttac ctgccgtttg
     1381 gcattctggt ctggatcttt ttcatctggt cgggcaagct gtttggcttc aacccggacc
     1441 tgggactcga cggatcgaca caacgccatc cctgaatagt gcatcatgcc cagcaaggtc
     1501 catttcccga cacctgtccc ccgcctgtac ctgcgggtga ggatgtggat cgcgctgata
     1561 ttcgccctct ccctggccgc tgacgtcgct gcggcaagca cactgaaaat ggctggccag
     1621 tggtacatca cctccacctg cgggcccgag gtttccctgc ccggtcacgg tacgccggta
     1681 cctgcgctcc gtcgcaccgg tggccagtgg tgctggctgg gaaacttcag gctcgaccag
     1741 ccgaccagcc tggtagtggc tttcgaaaac tccacggtta tcggccattt ccatcacacc
     1801 atctatgacg ccaaaggacg cgtgctggcc caggcagatg gcggcatcga aaacaccacg
     1861 cccaacccgt ttttcctgcg ccatggtcga ccattcacgc tccccgccgg cagctatcgc
     1921 cttgtaaccg aaatggactc gccattcctg ctggcccagc cgatcccgta cctggacacc
     1981 ctcgatcact accagcaggc aatcaagcgc ggtgacgcgc tgacactgct gtgcatgggt
     2041 gtcctgctgg gactgctgtt ctactatgcg gtactggcct ggattcgcaa caacccgacc
     2101 gatatctggt atgccctgtt cctgctcggc aacctgctgt acaacggcat ggcgttgctg
     2161 gtctatcccg aactgctcaa catccactgg ttctatctga tcagcttccc cattctgctg
     2221 tcgaatctgg cctatatact gttcgtcacc cgcctgctgg acatttcctg ggaaaatcac
     2281 aagcggctgt accagctggc catactggtg ctcggtatca tggtgctgtt cgcgatactc
     2341 gcgctgttca aacccaactg gtcactggaa ctcgaccgca ccggcgtggc gctgtttctc
     2401 agttacggac tgctggccgg cgtggtgcgc acctggcagg ggcattactc tgctcccatg
     2461 tacctcgcgg ctgtgctgtt gttcttcatc atgggcatcc tgtcgatatc gcttggccgg
     2521 atggcgggta tcagcgaaat cgatatcgaa cacctgggcc tgctcgccgt caccgtcgag
     2581 gctctgctgc tcgcgctggt agtcgcccgc cagttttccc agttacgtct gcaatatgag
     2641 catgcctacg cccatgccat gcacgacgcg ctgaccggac tgaagaaccg gcgtggtttc
     2701 ctcgaagccg gcattgccga aaccgagcgc tccaagcgtt acgaccaccc gctgtcgatc
     2761 atttatctcg acctcgacaa cttcaagcaa ctcaacgata cgcgcggtca cgacatcggc
     2821 gatgcagcgc tgcgcgccac ggccaaggca ttgcgcagcg tgctgcgttc caacgacctg
     2881 ctggcgcgcc tgggcggcga cgaattcgcc atcctgctgc cggaaatcgg caatgatgcc
     2941 gccatcgatg ccgggcgcaa ggtattcctt gccgtcaatg ccgcactgca ggatttcccg
     3001 ccagtcaccg ccagcatggg catcatccgc ttctctcgtg tcacgcgcag tttctcggcc
     3061 atgatgaaag tggccgacga actgatgtac gatgtgaaaa agaacggcaa gaacaacatg
     3121 aacttccgcc attacgactg aacgccatcg caggcgccgt acggtttgcc tgcaggtccg
     3181 tgcatgagcc acctttttcc tttccgtcag ttatactgta ttccatgatc cgtcacacct
     3241 cctgcgtccg ccggactgac cgggacgaca cgcgcatcgt gagcggatcc tgaccaggtt
     3301 tgatcaaagg gggagcccag aacttctgag cgctgcattc atggcccgaa cctccgtcat
     3361 ccacatgacc cgcgtgcagg ctcccgacag actgaccagc accatgctgc tcatgctgct
     3421 gacaggttgt tcgctggctc cgcactatga ccggcccgcc gcaccggtcg cagcaacctt
     3481 cgccagcgcc gggactgcca ccgcatcctc cagcatggca cgggtcgcgt ggcagcagtt
     3541 tttcaccgac ccgcggctgc ggcgactgct gcagctggcc ctggacaaca accgtgatgc
     3601 gcatattgcc atgcaacgcg tggaggaagc gcgagcactg tatggcatcc ggcgtgcgga
     3661 ccagctaccc ggcatcagcg ctggtctgtc agctacccgc atgcacctgc cgacaggcat
     3721 gtccatcggt ggttccgacc tcgattcaac ccagtatgca gtcacccttg ccctgagcag
     3781 ctgggaactg gatttctggg gccgggttcg caacctcaag gcggccgcac tcgaatccta
     3841 cctcggcgag gccgacgccc gacgcgccgt cgaactcagt ctgatcgcac aagtcgcaaa
     3901 cacctacctg ctcgaacgcg acctcgacga acgcatcgac accgcacgcc ggaccctggc
     3961 tacgcgcgac acgacctggc acatcatgca gcggcgctac gaagtgggtt cgatttcccg
     4021 gctcgatgcc agccaggcgc gcatcctgcg tgaccaggcc cgcgccgaac tggcggcgct
     4081 tgaacaacac agggaacgcg cccacaacgc cctcgtccag ttggtcgggg tgccgctgcc
     4141 ggatgaatcc gtgccgctgt cggccatcga aaccagtttt tccgccgata tcggcccggg
     4201 gatctcttcg gatgtgctgc tcgaccgtcc ggacgtgcag gccgcagaac accgcctcaa
     4261 agcagccaac gccaatatcg gcgttgcccg tgcggccttc tttccgcgca tcacgctatt
     4321 cggtggtctg ggcagttcca gcagcgcgct cgacggtctg ttcggcccga acacccagac
     4381 ctggctgttc gtgccttcgc tgtcgcagcc gctgttcgac aatggccgca accaggccag
     4441 cctcgatgtg gccgaagctc gccgcaacat ggctgtcgca gactacgaac gcaccatcca
     4501 gacggcgttt cgcgaagtgg ccgatgccaa cgccgatcgc cactggctgg cccaacaggt
     4561 ggcgctgcag cagaccatgc ttgcggatca gaacgaacgc gtgcggcttg cacagttgcg
     4621 ttaccagaac ggcatgtcgt ctttcctcga agtgctcgaa gccgaacgcg cccagtttgc
     4681 cgtcgaacag atgctggtgc agacacgacg cgccctgctg tcgggcacgg tcagcctgta
     4741 tgccgcactg ggaggcggca gcctgtccgg ccagccagcc gcctctttca acccacctgc
     4801 cggaacggac agacaatgat gcacacccta cccggaacct ggctgacccg ttcgctactg
     4861 ctggttgtcg tcatcgcact cgggagcatc gcctgggttc aataccgcaa tcacgaacat
     4921 gacatcggac tggtcagcgg caacggccgg atcgaagccg tcgaaatcga catcgccgcc
     4981 aaaacgcccg ggcgaatcag gacgatgacg gtacacgaag gcgaattcgt caagaccggc
     5041 cagatcatgg ccagcatgga taccgaagcg ctcgatgcgc agcaacgtca ggccgaagca
     5101 cagttgcgcc aggcgcaaag cggcgtgacc atcgcccgca gccagctgag ccaacggcat
     5161 gccgaacagg cagccgctct ggccgtactg gcccagcgca gagccgaact ggaagtggcg
     5221 cgcaagcgtt cctcccgctc gtcgatactg gcgcaggagg gtgccggctc acaacaggag
     5281 gccgatgacg accatgcccg gacgcaaagt gcatctgctg cactcagtgc ggcagatgca
     5341 caggctgcgg ctgcgtccgc cgccattgcc acggcccgcg cacaactgag cggtgccgaa
     5401 tcagcggtca ccgcggccac ggccgccatt gcccggatca acgttgaaat cggcgacgcc
     5461 acgctcaggg caccacggaa cggccgggtg caatacatcg tggcccagcc cggcgaagtg
     5521 gtcggcgctg gcggtcgggt gatcgacatg atcgacctga cggatgtcta catgaccttt
     5581 ttcctgccca ctgctgctgc cggcaagctg gcgctgggag acgaggtgcg gctggtactc
     5641 gatgccgcgc cacagtatgt catccccgcc cgcatctcct ttgttgccga tgtagcccag
     5701 ttcacgccaa aaaccgtaga aaccgaagca gaacgcgaaa aactcatgtt ccgcgtccgg
     5761 gccagaatcg accctgacct gctggcaaaa catctggtac gcatcaaaac cgggctgccg
     5821 ggcatggcct atgtccgcct cgacccatcc gtaccatggc cgacgcggct ggcgctgaaa
     5881 ctgccacaat gatcacgaca cagcgcgagt ccccggatgc tcccatggcc cgcctgcatg
     5941 gggtcagcct gcaatacggg cgcacccggg cactggacgg catccatctc gaactgccgc
     6001 gcggatgcat gatcggactg atcggtccgg acggcgtcgg caaatccagc ctgcttgcgc
     6061 tcattgccgg tgcgcgtgcc ctccagaccg gacgcatcga ggtgatggat ggcgacatgg
     6121 ccgatgcggc ccaccgccgc gcactttgcc cacgcgtggc gtatatgccg caagggctgg
     6181 ggaaacatct gtatcccacg ctgagcgtct tcgaaaatat cgattttttt gcccgactgt
     6241 tcgatcagag tcgcgccgaa cgcgagcagc gcatcaccta tctgctccag gcaaccgggc
     6301 tcgcaccatt ttctgatcgt gcggtgggca aactgtccgg tggcatgaaa caaaaacttg
     6361 gcctgtgctg cgcactgatt cacgaccccg acctgctgat actcgatgaa ccaaccaccg
     6421 gagtcgatcc gctctccagg cgccagttct ggaagctggt cgaacgcatt cgtgcccgcc
     6481 gtcccggcat gagcgtgctg gtagccacag cctacatgga agaggccgcg cgtttcgact
     6541 ggctggtcgc catgcatggc ggtcgggtgc tgtctgccgg caaacccgac gaactgctgg
     6601 ccagaacgca cgccacgacg ctggaaaccg cattcatctc cttgctgccc ggtgcacgac
     6661 acaccggata ccacccggtc gtcccgcccg tccgcgaatc aggcgacggc accccaccgg
     6721 ccatcgaagc acacaatctg accatgcggt tcggcgattt cgttgccgtg aaccatgtca
     6781 gctttcgcat tggccagggt gagattttcg gctttctcgg ttccaacggg tgcggcaaga
     6841 ccaccaccat gaagctgctc accggcctgt tgcctgccag cgaaggggaa gcaaaactgt
     6901 ttggcaagcc agtcgacgcc aatgacatcg acacccgacg ccgcgtaggc tacatgagcc
     6961 agtccttttc gctctatggt gaactcacgg tgcgccagaa cctggaactg catgcccggc
     7021 tgtttcgcat gccagcagcg cacattccgc cacgggtggc ggaaatggca cagaaattcg
     7081 gtcttgatga tgtgctcgac atgctgcccg agtccttgcc actgggcgta cggcagcgcc
     7141 tctcactggc tgtggcgctg attcacgggc cggaaatgct gattctggac gaaccgacct
     7201 ccggtgtcga cccgatcgcg cgcgacgatt tctggcgcat cctgatcgat ctgtcacgca
     7261 atgacaaggt gaccattttc atttcgacac acttcatgaa cgaagccgag cgttgtgacc
     7321 gcatttcgct gatgcacgcg ggcgaagtac tgatcagcga cacgccggcc gccatcatcg
     7381 ccgccagcaa tgccgactct ctggaaagcg ctttcattca ccacctggaa agcgtggcag
     7441 gcggcgaaga cctgtccatc gccgaccttg ccggcgcggt acagtccacg acacttccgg
     7501 cggacaacgg acagggcgaa aagcgtttca gcgtgcggcg catgttcagc tacacgcgtc
     7561 gcgaatcgct ggaactggcg cgtgacccga tccggctggc gctgtcgctg ctcggcagcg
     7621 tgatcctgat gatcgtgatc ggctacggca tcaacctcga tgtcgacaac cttgcctttg
     7681 ccgtgctcga ccgcgaccag acgccaccca gccgggatta cgtgctcaat ctggaaggct
     7741 cgcgctattt catcgaacgt cctccgctgc gcaattacgc cgatctcgac cgccgcatgc
     7801 gcacgggcga aatcagcctg gccattgaaa ttccgcccgg ttttgctcgc gacgtggcgc
     7861 gcggcagaca agtgtcgatc ggcgcctggg tcgacggtgc catgcccatg cgtgccgaaa
     7921 cgctggacgg ctatgtacag ggcatccatt accaatggct acaggcccgc gccagcacgg
     7981 cacttgggcg tgccggcacc gatggcctgg tgcggattga aacccgtttc cgttacaacc
     8041 cggatgtcag gagcctgccg gccatggtcc ccgccgtgat acccttgctg ctgatgctga
     8101 tcccggccat tctgagcgca ctgtcggtgg tgcgcgaaaa agaacttggc gccatcgtca
     8161 acctgtatgt cacgcctgtc acgcgcagcg aattcctgct cggcaaacaa ctgccctaca
     8221 tcggcatggc catgatcaat tttctgctga tgacgacatt ggcggtcttc ccgttccgca
     8281 taccgttcac tggaagtttc ctcaccctga ccctgggtgc cctgctgtat gtcacctgct
     8341 ctaccgccat ggggctcgtg gtatctgcat tcacccgcag tcaggtgacc gcgctgttcg
     8401 ccaccgccgt actcaccatc ctgccggcga gccagttttc cggcatgatc aatccggtat
     8461 cctcgatgga agggctggga agatggatcg gcgagatcta cccgaccacc tatttcatca
     8521 tcatttcacg tggcaccttt tccaaggcac tgggtttttc cgacctgcag gacgcaatcc
     8581 tgccgctcgt cgccgccatc ccggtactgc tcggtcttgg cgtgctgctg ctcgcaaaac
     8641 aggagcgctg accatgcggc tgcacacgat caccaccctc ggcgtcaaag aactgcgcag
     8701 cctgacccgg gaaccgatga tgctgctgct catcgcctac tcattctcgc tcgccatcta
     8761 tgccgcagcg acagtgatgc cggaaacgct gcagaatgcc cccatcgcca ttgtcgacga
     8821 agaccattca cctctgtccg aaagcatcgt cggccagttc acccctccat ttttccaccc
     8881 gcccgagcag atcacccagg ccgagatgga cacccgcctg gacgccggca ccgacacgtt
     8941 tgcgctcgac attccacccg gtttccagcg cgatctgctt gcacgccgac agcccgccat
     9001 acaactcaat gtcgatgcca cccgcatgac gcaggcattc tccggggcaa gctacatcca
     9061 gagtatcatc cagggcgaag tggaccattt tgccctggat caccagatgc cgatccacct
     9121 gccaccggtc ggactcgacc tgcgatcgcg cttcaacccg gagcttaacc aggcatggtt
     9181 cggctcagtc atggaactca tcgaacaggt aacgctactg tcaatcatcc tgtctggcgc
     9241 cgcactgatc cgcgaacgcg aacacggcac cgtggaacac ctgctggtga tgccggtgac
     9301 cccgtttgaa atcatggcaa gcaaggtttg gtcgatggga ctggtggtat tgctggctgc
     9361 atcgttttcg ctcaccgtgg tggtcgaagg catcctcaag gtaccgatac agggatccat
     9421 tccgctcttc cttgccggaa cggcactcca actgttcgcc accaccgtgc tgggcatcta
     9481 cctcggcacg gtggcacgca ccatgccaca gttcggtctg ttgctgatgc tggtactgct
     9541 gccgctcatg gtgctgtccg gcagtttcac ccctcgcgaa agcatgccgg tcggcatacg
     9601 cgacctcatg ctgctgacgc cgaacaccca ttacgttctg ctcacccagg ccattctgta
     9661 ccggggtgcc gggctggatg tcgtctggcc gcaattcgcc agcctggccg tgatcggcgc
     9721 ggtactgttc gccctgtcac tggcacggtt ccgcagtgcg ctcgcgatca tgaaccagta
     9781 accaccgggc agacgccagc cattgcccca tgactgccca catcgggcag ccggcgccac
     9841 gctatttgtg ctggatcacg acaaagttgt ccaatgcgcc gagcaccttg taatggtttt
     9901 gagccatgtt cgtttcatac aggctgaaca acaggaaatt ggtccgtgtt gtgaaattga
     9961 cgatcaggcc actcgccaga ccgctgaaaa gcgtgctcac ggtttgtgtc acaacatcgc
    10021 tgttttgcga attctgcacg atttccttgc ggagatactg ttcgtagtcc ggcatgtcgg
    10081 gattggtaaa ggcaaacaac gctgcgatac cgacagtcgc catgatgatt ctctgtttca
    10141 ttgatggcct ccagatccgt tcccgacccg cgcatgacca gtgtcggggg cacaaccgga
    10201 cacacgggca tccaccattc agctctctca cccggccgtg cgctgattga caataaattt
    10261 caggcattct agcattcgac catgaaccgc ttccatctga tttcccgacg agaccctgct
    10321 gcccgcaccc aacggggcgg tcgtgtcatg ttcagggaaa atcaacgaag cgtgcattct
    10381 ggaattcgat gcacctagcc ccccaggcaa cccgaatccc tgcaaggcca cggctgaccc
    10441 ccgtggcctt tttgtttgca ctgcaccctt gaggagacca tgcatgacgc tgcccgccag
    10501 ttttccaccc gcaagtctga tgcccattgc cccccggcca gagcttgttt tcgtacgtgg
    10561 ggaggccagc catctgtggg accagcaagg acgtcgctat cttgactggg tgcaaggctg
    10621 ggcggtgaac tgcctcggcc atgcccccgc cgtgattgca gacgcgctct gtacccaggc
    10681 gcataccctg atcaatccga gcccggcatt ttacaatgca cctgcgatcg aactggccgg
    10741 gctgctcacg caatccagtt gcttcgacca ggtgttcttc ggcagttccg gcgccgaagc
    10801 caacgaaggc gccatcaagc ttgcgcgcag atggggaaag cgctacaaag acggggtcag
    10861 tgaaatcatt acgttcgtca acggatttca tggccggaca ctggccacca tgtccgcaag
    10921 tggaaaacca ggttgggata cgctgtttgc accgcaagta ccagggtttc ccaaggccat
    10981 cctgaatgac ctggattccg tcagcgccct catcggcccg cagactgcgg ctgtgatgct
    11041 ggaaccgatc cagggtgagg caggggtcat tccggccacg ctggaattca tgcgcggatt
    11101 gcgggcgctg tgcgaccggc acggactgct gctcatcgtt gacgaggtgc aaaccggatg
    11161 tggccggacc ggaacgttgt tcgcctacga acagtacggc atcgaaccgg acatcatgac
    11221 cctgggcaaa gggctgggtg gaggcgtacc catttcggca ttgctggcga agcaatcctg
    11281 ctcctgcttc gaaccgggcg atcaaggagg aacgtatgcc ggcaatcccc ttgtctgtgc
    11341 cgcagccagg gcagtcatcg acacgttgac cacagatggc tttctgagca gaagccgtgc
    11401 cgtgggggat cagttgcggg cggagctgaa ctccgtttcc cgtgaattcg gactgggcga
    11461 agtgcgcggc aacggactct tgcttgccct ggaactgaat tcagatctcg ctgcgcaaat
    11521 cgttgccaac gcgcgcgcga acggcctcct gctcaacgcc ccacgccccc actgcctgcg
    11581 tttcatgcct gcactcacga caacacccgc agaagtggaa gacggcattg ccatattacg
    11641 ccagaccatt tcggccatcc tcgccggagg gtaatccccc catttttagt tggggtcaaa
    11701 agtagattca tgcggccatg gccagccgct gtttcggtgt gatac
//
LOCUS       DAIANL010000068         3560 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1514352, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000068 DAIANL010000000
VERSION     DAIANL010000068.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514352"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          718 a         1183 c          977 g          682 t
ORIGIN      
        1 ctcgcaggac ggctctgccg acacgttgcg cgaatgggtc cgcgcgcatc aggctggcaa
       61 ccgtgtcagc gtgctcgact cgggattcaa cggcggtttt tctttcggta acaatttcgg
      121 catccgtgcc atgccggcac agtactacct gctgctcaac agcgatacgc tgatccgccc
      181 gggtgcaatc cacaccctgc tggacgccgc gtaccagcac cctgaggcag gcatcctcag
      241 cccgcgcctc gaatggccgg acggtaccgg acaggaaagc tgtttccgct acccatcacc
      301 gtggagcgaa ttcagcgatg ccgccaagac cggggtactt gaccgcctgc tcgcgcgcca
      361 taccgtggcc atgccggtgc aggacagcat cgcccgcccc gaatggacca gctttgcatg
      421 cgtactcatc cgcagcacgg tgttcaacga gatcggcctg ctcgacgaag gttatttcat
      481 gtatttcgaa gacgtcgaat tctgcctgcg cacccgtcat gccggctggg acatcgtgca
      541 cgaccctgcg gcgcatgtgg tgcacctgcg cggcggcagt tcaccgctca aggaacaaac
      601 ccgcctcagg cgccgcttgc cacgctattt ctaccagtcg cgcacccgct tctaccgtca
      661 aagcgccggc atactgggcc cgacagtcgc caacctgatg tggtggctgg gcaggctggt
      721 gtccggcccg cgacaactgc tgggccgcga tgacaagcgt ccgccggaac ggcagggaca
      781 cgacatctgg accaactggc tccatcccct caaaccctgg gccccaccgc gaacatgaat
      841 gccatgccac ccacatcccg cctgccgcat ttcgtcatca tcggcgccat gaaatcggcc
      901 accaccacct tgcaccgtca actgtccgaa cagcccggca ttttcgtgtg cacgcccaag
      961 gaacccaatt tcttcagcga cgaccgcgaa tacgcccgtg gtctggcatg gtatgccgga
     1021 ctgtttgctg acgcgccgca caccagtctg ctcggtgagg ccagcacgca ttacaccaaa
     1081 ctgccgaccc acccggacag cgtgccaagg atacggcagc atctgcccga agcgcgctgg
     1141 atctacgtca tgcggcaacc ggtcgaccgg ctggtgtcac atttcatcca tgaatggagc
     1201 atgggggttt accgctgcgg tatccgcgaa gccatcgacc gctatccaga actggtagac
     1261 tacggccgct atgccatgca actcgaaccc tggttcgaag cctttggccg cgaccgggta
     1321 ttgccggttt tcttcgaccg gctgcttgca gctccgcagg cagaactgga acgcatctgt
     1381 actttcatcg gttaccccgg tcaaccgcaa tggatacagg agttaaaagc ggaaaatgtc
     1441 tccagcgagc gtatccgacg ctttccgggc taccgcgtgc tgatcgaatc ggcagccgct
     1501 acctggctgc gccgcaatct ggtaccgcaa ggcctgcgca acagggtcaa atcccgtctg
     1561 cgcatgcagg aacggcccca gctcgacgac gcaacacgcg ccgaactcga aacccgcttc
     1621 gaccccgatc tcgcccgcct cggacaatgg ctgggcaccg aactcgactg ccgcaacttc
     1681 agacaggtca ccgcggccgg gccattgaac tgggtcgctt cccatgactg aggcacccgc
     1741 cacccacagc atcggcgtcg ttgccatcgg ccgcaacgag ggcgagcgtc tgcgcgcctg
     1801 cctggaatcg gtcattcggc gtgccgaacg ggtcatctat gtcgattccg gttcgaccga
     1861 cgactcggta tccatggcgc gctcgctcgg tgtggaagtc gtcgcactcg atcttgcccg
     1921 tccatttacg gcggcacggg cacgcaacgc cggattcgcc cgtttgcgcg aatgcctgcc
     1981 agacgtacac tacgtccagt tcgtcgatgg cgactgcgaa gtgcgtccat catggctgga
     2041 acaggcagca gcatttctcg acagccgccc gcagtacgca gtggtctgtg gtcgacggcg
     2101 cgaacgctac ccggagcatt cgccctacaa ccgcctgtgc gacatggaat gggatacccc
     2161 ggtcggcgaa gcgcgctact gtggcggcga tgccatgatg cgcgccgatg cattgcaggc
     2221 tgtcggcggt taccgtgaat cgctgatcgc gggcgaagaa ccggagctgt gcgtgcgcct
     2281 gcgtgctgtc ggctggaaaa tctggcgact ggatgccgaa atgacgctgc acgatgccgc
     2341 actgtcccgt ttcggccaat ggtggcagcg caccaaacgt ggcggctatg cggccgcaga
     2401 agccgaacac ctgcacggcg cgccgcctga acggcacggc gtgcgccagg catggagcgc
     2461 ccgcctgtgg ggtatcggcc tgccactgct ggccatcgca ccgctgccct ggaccggccc
     2521 ggccagcctc gccatcctgc tcgcctatcc gctgcaaacc gttcgactgg cactgcgcgg
     2581 ccaccgttct gcacgcgaca actggctgca ggcaggcttt ctggtattgg ggaaatttcc
     2641 cgaagcggcc ggccagctca agttcctcta caaccgcttg tccggcaaga ctgcccacct
     2701 gatcgaatat aaatgacccc ctcgcccgac acggaccaga caccacccag cgtgccgacg
     2761 ctgcgttcgc ccgaacaggc gcgaaagaca cgccccatgc tcaaaaaccc tgccacgcgc
     2821 ctgtaattac gacatgcgca ttgattgtat atggttaatt gaatatgaag attacgttta
     2881 tcctgcctac cgtaaatatg agcggtggta taaaagtcat cgccatctat gcaaaaaaac
     2941 tggccgatcg tggtcacgaa atcgttctga tttccccgcc accccccgat ctgcccctga
     3001 aacgcaaaat aaaatccctg ctcacaggca aaggctggcc cattgctcat ccctttccat
     3061 cgcacctgga taacctcggc ctggatcacc ggttgctgga tacacatcgc gcggccaccg
     3121 atgcagacgt acctgatgca gacgtcgtca tcgccacatg gtgggaaacg gctgagtggg
     3181 tcaacagcct ctcaagcgcc aaaggcgcaa aagtctattt catccagcac catgaattgc
     3241 atgatttcat tcctgccgcg agcagtcggg ccacttacat gttgcctctg cataaaatcg
     3301 tgatcgccag ttggttacga aacctcatgc gcgacgaata tttcgaccac gacgtcgacc
     3361 ttgtaccaaa cagtgtcgac aaaacgcaat tttattctgc cgttcgtgac aagcagcctg
     3421 tcccgaccat cggatttttg tatcatacga gccagtataa aggcgtggac atttccttga
     3481 aggtcattcg tcagttacgc gacatctttc cggatctgcg ggtcatcgct ttcggttcaa
     3541 tcctgcctcc cgcactggct
//
LOCUS       DAIANL010000069        13185 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3147963, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000069 DAIANL010000000
VERSION     DAIANL010000069.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13185)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3147963"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2840 a         4321 c         3592 g         2432 t
ORIGIN      
        1 cccggctttc ggaaacctgg cgcacgcgac aattcgaata ccaatggttg cgcgccctgt
       61 cgggacacta tgcggatttc tggcaggcca cggacgatgc gctggtgttc tcgtgcgaac
      121 tgctcaaact ggatctgacg cccgagaaac gccagcagct gatggcggct tatcttgatc
      181 tcaaagtctg gcccgatgtg ccggcagcct tgcaaacgct caaatccgcc ggtctccggc
      241 tgggaattct gtccaacgcc acgccacgga ttcttgacgc cggcatcagg aactccgcgc
      301 tcggggatct gtttgaacgg gtgatcagta ccgatgccat taaaacctat aaacccgacc
      361 cgcgcgctta ccggatggcg atcgatgcgt ttggcctcga acgcgaggaa atcctgttcg
      421 ttccgttcgc gggctgggat gctgccggag caaaaagctt cggctatacc accttctggg
      481 tgaatcgcct gaacatgccc gccgaacagc ttggcgagaa ggcagatgcc gcaggccgca
      541 cgctgacgga tctggtcagg ttcatgcagg cagacaaccc ggtcaaagca ggatgaccgt
      601 caggcatgaa ccggccggat gcgcgtctga cacaaaaatg acatcgcacc aggcgaaaat
      661 ctgcaggaac caggccatac ccgagggacg ccgcccatga aatacgccat gcatgtgcgt
      721 aaaatccgtg ccgatgaagg cggcttgctg cttcagatgc gcatcgccgc cctgaccgag
      781 gcgccgtacg cttttggctc caggcttgaa gacacccagg cgcaaccact ggcatcgttc
      841 gagtccgccg ctgcaaaaca tgcggattcc gaagtgtcca cgacgttctt tgcgtttgat
      901 ggcgtcaacg cggtcggtct gatcggcgca tttcgggaaa cctcgccgcc gcaacgctgt
      961 ttcatatgct cgctctggct ggcacccgga atcggggcac tgtcttcgct tcagaactgg
     1021 tgcacacggc ctgcgcctgg ctgcatcagc gtttcagtga ggatatcttt gcatgggtgg
     1081 ccgatgcgaa tccgcgcgcc atggcgtttt acaggaagtc aggattcata ccggcaggcg
     1141 agtgccagcc cttgccttcc actccgtccg catcggaaac cctgttgcgg ctcggtaccc
     1201 ggcgccaggc gatgacgggc tgacggatgc tcaggcaaat ccgcagaaca catcgcgcat
     1261 caggctgata agccgcagcg tacgcgtgtc gcccacacga tagaacacac ggttggcatc
     1321 cttgcgtgta cgcagcacac ctttgtcgcg taaaatggcg agatgctgcg agatattgct
     1381 ttgcgatgta ccgacctggt ccacgatgtc ctgaacgctg acctcccggt caccaagcac
     1441 gcagagtatc ttgagccgca gggggtgcga catcgctttc atcgcacgcg acgcctgctc
     1501 gatgtgttca tccttgtcta tcagatcgat atgatcgaaa tcaagcacga gtcgggacat
     1561 agttcctgca atccaattcc tgtatgacag tatgattata atgcaatcgc cagggaaata
     1621 taataaaatc cgggcgaacc gccccgaacc accggccggg ccagaatccc ggcatgcata
     1681 gcgagtccag attgaccccc gtactgctca tcattctcga cggtttcggc tgtcgcaaat
     1741 gccataccga caatgcaatc gccctggctg acaagcccca ttggcttgat ttctggcaac
     1801 accacccgca tacgctgatc caggcctcgg agtctgccgt tggcctgcct tcaggccaga
     1861 tgggcaactc cgaagtcggc catctcaaca tcggtgccgg ccgcgtggtc taccaggaat
     1921 ttacccgcat cgaactcgcg atcaagaccg gcggcttttt ctccaacccg accttctccc
     1981 gttgcatcga cgatgccaaa cgcaacggcc gtgcgctgca catcctcggt ctgctttccg
     2041 atggtggcgt gcacagccat gagacgcaca tccacgccat gctcgaaacc gccgcacgtg
     2101 ccggcatgcg cgatgtctac ctgcacatct tcctcgacgg gcgcgatacc ccgccacgca
     2161 gcgccgaaac ctacattgcc cggctcgaag acaagatccg tgaactcggc gttggccgca
     2221 ttgccaccat gatcggccgc tatttcgcca tggaccgcga ccgccgctgg tcacgcgtac
     2281 agaccgcatg ggatctcatt acccgcggcg acgccccgtt cgaggcagca gacgcacaaa
     2341 ccgggctgca catggcctat gcgcgcggtg aatcggacga attcgtccag gcgacgcgca
     2401 tcggcgaagc ggtgcgcatg caggatggcg acagcgtcat tttcatgaac ttccgctctg
     2461 accgggcccg ccagctcacc cgtgccttca ttgagccgga tttcagtgaa ttcgaccgca
     2521 agggccaccc tcgcctgacc gacttctgta ccttgaccgg ctacagcgac gacttccagg
     2581 tttcggtcgc ctttcccccc gagcgcatcc gcaacggctt cggcgaatac atttcacgcc
     2641 ttggcctcaa acagttgcgc atcgccgaga ccgaaaaata cccgcacgtg acgtttttct
     2701 tcaacggcgg cgaagaaacc gtctatcagg gcgaagaacg cattctggta gcctcgccca
     2761 acgtggccac gtatgaccag aaaccggaga tgagtgccta cgaagtcacc gacaaactgg
     2821 tcgaagccat cggccagcat cgcttcgacg ccatcatctg caattacgcc aacgccgaca
     2881 tggtgggtca caccggcaat ctcgatgccg cgatccacgc catcgaggcc ctcgacacct
     2941 gcctcgggcg cgtcattcct gcgatgctcg aagcgggtgg agacatcctc atcacggccg
     3001 atcacggcaa cgccgaactg atgatggacg aacacaccca ccaggcatac accgcgcaca
     3061 ccaccaacct ggtgccattg ctgtacatcg gcaaacgcac ggcgacgctg gcggccaccg
     3121 gtgcgcttga agacgtcagc cccaccatgc tggccatcat gggcattccc cagcctgcgg
     3181 agatgaccgg caaatcactg atccagttcg agtgaaacgg cacgggccca cgatcttcct
     3241 gctcatcgcc accctgtcag gtggtctgtc ggcgcatgcc gccacggacc gcgccgaact
     3301 gaaccgcatc cacaagcaga tcagccatct gcgcgagcaa caccagacca cggagaacaa
     3361 ccgcaaccag gccgcggatg cgttgcgtca atcggaactg gccatcagca ccaccaaccg
     3421 ctcgctctac gaattgcagc agcgttccga ccagacagaa gccagcctgc gtgcgctcaa
     3481 cgcacgcatc ggcacagtaa gacaatcaat cagtcagcag aaaacgcgtc tggccgcgct
     3541 gctgcgtcag agctatttcg acagccaggc caacgacgcc cacctcctgc tcgacggcgc
     3601 acctgccggc cagaccgcgc gcgacctggt ctactccggc tatctcggac gcgaacgcaa
     3661 ccggatgatc gccgacctgc gccacaacgc cgacacgctg aaccagctca aggcccgtca
     3721 gcagcaactt caggccgacc tcgacagcca gcgcagcgag cgtgagcgtg aacgtcaggc
     3781 gctgcaggac gagcgccagc gccgccagca ggtactggcc agtctcggaa cccagatccg
     3841 ccagcaggaa cagcaactcg ccaccctgga ggaaaacgag aaacgcatca cccgattgat
     3901 ggaacaactg gcgcgacagc ggcgccatcc ccggccagct cgccatgcgc gcgaacctgc
     3961 cgccggcgcg ccagtcaccc cgctgcctca gcctgacctg ccagccacag gactcggtct
     4021 gctcaaggga cggctgccca tgcccacaca cggggaactt attcaccgct tcgggactcc
     4081 acgtgaagcg gggggaactc tctggaaagg tttgttcatc aaggcgccgg ctggccagtc
     4141 cgtggtcagt gtcgctgccg gtcaggtggt gtttgccgac tggctgcgag gctttggcaa
     4201 cctgatcatc atcgaccatg gtggcggcta catgagcctg tacagcgaca atgaaaccct
     4261 gtataaacga gtcggcgatt tcgtcaagtc cggtgaagtg attgccgcag tcggcaacac
     4321 cggaggcagc aacgaaacag gactgtattt cgaattgcgg taccggagcc aggcattcga
     4381 cccggatccg tggcttgtcc gccaataaac aggaaccaca gcacaatgcg caccaccctg
     4441 caaaaaatcg gcctgattct ttcgggcatc cttcttggtg tcaccctgac cgcatatctg
     4501 cctgccatcg ccgaccgcga ggccgccagt gccacgttgc cgctcgacga actgcgcgag
     4561 ttcagcgaag tgttcggcaa gatcaaaagc gactatgtcg aaccggtttc cgacaagaaa
     4621 ctcatcgacg acgccatcac cggcatgctt gcaggtcttg acccgcattc ggaatacatg
     4681 gatgccgatg ccttcaagga actgcaggac ggtacccagg gccagtttgg tggtctgggc
     4741 atcgaagtcg gcatggaaga cggactgatc aaggtcattg cccccattga ggacaccccg
     4801 gcacaaaagg ccgggatcaa gaccggtgac ctgatcgtca agctggacga caccccggtc
     4861 aagggcatga ccatcaacga ggccgtcaag cgcatgcgtg gcgaacccaa cacgccgatc
     4921 gtgctcacca ttgtacgcaa gggcgtgaac aaaccgtttt ccgtcaaggt gatgcgcgct
     4981 gtcatcaagg tcaagagcgt caaataccaa ttggtcgatc ccaactacgg ctacgtgcat
     5041 atcacccagt tccaggaaca cagcggcgaa gacctggtca aggccatcaa cgcgctaacc
     5101 gcacagaaca aggctccgct caagggcgtg gtactcgatc tgcgcaacga cccgggtgga
     5161 ctgctcaaca gcgccgtagc cgtagccgcg accttcctca agccggacag cctggtggtc
     5221 tacaccgatg ggcgcaccga ggactccagg atgcgtctga ccgccaaccc ggaaaattac
     5281 ctgcgcggcg cgcaagattc cgactacctg cgtgaccttc ccggctggat caagaccgtg
     5341 ccgatggtcg tactcatcaa cggtggctcc gcatcggctt cggaaatcgt ctctggcgcg
     5401 ctgcaggatc acaagcgagc ggtcatcatg ggcacacagt cgttcggcaa gggatcggtg
     5461 cagaccatcc tgccactggg taatggcacc ggcatcaagc tgaccacggc acgctacttc
     5521 acccccagcg gccggtcgat ccaggccaaa ggcatcacgc ccgatatcgt cgtcgaggaa
     5581 ggcaaggtcg taagcgacgg tgacagcgac ggcggcctgc tcaacatcca cgaagccgac
     5641 ctggaaaacc acctggccaa cccgaacccc ggcaccaaag gcagtggcaa gaccccgccg
     5701 cgcatcgttc cgcccgagtc tgctgccaaa acgccggcag cgcctgccaa tcctgacgag
     5761 gtcgtgcaca agaacgactt ccagttgacg caggcactgg acctgctaaa gggcatcaac
     5821 attctggccc acgacacctc ccaggccaag aattgacctg aacggcaacg gcgccgagtt
     5881 cctggcgccg ttgccgcctc tacgccaggc tcccgcatac cgtggatgac gcacaactgc
     5941 tgcattacag ccgccacatc ctgctggaag ggttcgacct ctccggacag gaacggctgc
     6001 tgtccagccg catgctgctg gtcggcgtcg gtggcctggg ctctgctgca gccctgtatc
     6061 ttgcagccgc aggtgtcggt cacctgacca tcgccgacgc agatgtggta gaagccagca
     6121 atttgcagcg gcagatcatt caccgctaca ccatgctggg cagcaacaaa gcggtttccg
     6181 cagtcagcgc cgcacgcgcg atcaatccgt ggatcgaact ggtcgccctg cccgaacgtc
     6241 tggctgccga acggctcacc gcgctggcca gcgaacatga cgtgatcatc gatgccagcg
     6301 acaatttcac cacgcgccac gacatcaacc gagcctgcgt gcagctgcgc aaacctctgg
     6361 tttccggcgc cgcgatccgc tacaccggcc agctcgcgac tttcgacctg cgcgacccgg
     6421 acagcccgtg ctaccactgc ctgttcgccg atactggcgc gggcaacgat acccgctgca
     6481 gtgaaaccgg cgtattcggg ccgctggtgg gcatcatcgg cgcagcccag gccgcagagg
     6541 ccatcaagct ggtcaccggc atcggccatc cactctgcgg ccgattgctg caactcgaga
     6601 tccgtgacat gcaatggcgc gaatcgcagt tcagccgcga tccgcattgt ccggtgtgca
     6661 gacagcatgc tgctggctag aaacgaaaca gcgtactgtc ccagcggtgg atcggcaccg
     6721 gttcgccgaa gagaaagccc tgatagacgg cacagccgcc catcgacagg aattcctgct
     6781 gcaattcgtg ctccacacct tccgccacca cctccagctt gagaaagcgc ccgacggcaa
     6841 gcatggctcg caccacttcg tcaccatgcg catcctgacc aatgtcttgc acgaacgagc
     6901 ggtcgatctt gatctgctcg aacggcagac gcttgagcag cgacagcgat gaatagcccg
     6961 taccaaaatc gtcgagactg aaatgaacgc cctggtcatg caacatccac atccgtcgaa
     7021 tcgccgcttt cgtatcatgc agaatcacct gctcagtcag ttccagcttc agcaacgccc
     7081 tgtccacctc cagttcctgc agcaacgcaa gcaggcggtc ggcgaactcc ggatgatgaa
     7141 actggcgcac gctgatattc accgcaagcc gcaactgtcg cgtcaggcca ttggctgacc
     7201 attgccgcaa ctgttcacac gcctgccgca acacccattc gccaatcacc acaatgagtc
     7261 cgctgtcttc ggccaggccg atgaactggc ccggacacag ggtgccgcgc tcaggtgact
     7321 gccagcgcaa tagtgcttcg gcacccacca gacgaccgaa ttgatccacc tggggctgat
     7381 aatgcacctc gaactgacgc aattcgagtg cagcccgcaa ctcccgttcc atgccagtac
     7441 gcgaatcgat tgccgcctgc atctgcggat caaaaaagcg tgcggtgtta cgtccggcgt
     7501 ctttggcctg ataaagcgcc atatcggcct gcttgaacag cgtttccaca ctgaactggc
     7561 gaccgcggaa caacgacagt ccaatgctgg ctgaagtggt gtattcggtg taaccgctgc
     7621 cgatggaaaa cggattgccc agcgccagcc ggatcttttc tgccacggct tcggcccgtg
     7681 tagccgccac atccgcctgc tggccaagat gctgcagcaa cacgacgaat tcgtcgcctc
     7741 ccaaccggct caccgtgtca tcctggcgca cacaggtctg caagcgttgc gccacgccga
     7801 ccagcagacg atcgcctgca tcgtgacctg cgctgtcatt gagggtcttg aaatgatcca
     7861 gatcgagcat catcagcacg gcaaattcat cgttgcgatg gctggcgctc tgggcctgcg
     7921 ccagacgatc gatgagcaat ctccggttcg gcaagccggt gagcacatca aaataggcca
     7981 actgacggat ctcgtcctca gccagcttgc gcgccgtgat gtcacgtacc gtcacgcgcc
     8041 ggccacggta gatccctgca tcatccacca ccggccggca acggtggctg acccacaggg
     8101 tatgccctgc cttatgcacg atgcggattt caagttcctc gattcgcaga tcgccgtcac
     8161 tctgccggac atcctgtgca tgcttttccc actgcggaag gtcttgcgcg taaatgatgc
     8221 ggcggacaag gccaggatcg gtatagaatt cttccgcacg gtagcctgtc acgcgttcga
     8281 cggatggggt catgtagaca aacccggaat ccggctgaac ccagtactcc cagtcggaag
     8341 tccagtccac catggtatgg aacttgattt cattctcgcg caccgcgagt tcggcgcgcc
     8401 ttcgctcggt tacgtcctgt cccaccgcca ccagcagact cggcgtgccc acggcgattt
     8461 cacgaaacag cgaaatccgc caactcaccg tacgccgtcc acccaacctt ccggcccagt
     8521 ccatcacgaa tggcggagtc tcttcgcctc gcaaaaaagc attgaaacgc ttgcgcaacc
     8581 agtcgtgttg ctcggctggc agaaagcgca gccagaaaaa cggctggctg cggacttcgt
     8641 caaagccata gcctgacaac agttcggctg catgattgaa acgcacgacc accccaccgc
     8701 ggtcgatcac catgacgata tcatcggtgg tggcgaaaat ggcatcttca agccggcgtt
     8761 cttccgccag tacacgctca cagtgcacat ggacctgtct gcgctttcgc cgacagtgca
     8821 agcgatgaat gccgtgtgcc gtcacggtca gggcagcaat catccaccct gcgacagaaa
     8881 tggacgaatg ccaccaggcc atgccgtcag gatgaatgtg cagaaccatg actgatcaca
     8941 ccaaatgaat acgcgctaac ctggcctcgc aggcaacgcc ggaatccgtg ccgcatcagt
     9001 tcgctgcgtc aaacgaagac aacccaccct attgttccgt ccaacaggct gccacatgtt
     9061 catagtaacc gcattctgaa gatggtttca accgcaatcg catgtcggga atgcactcgc
     9121 gtttaccgtg acatacggca caaggcatgc agagcagcct gcggttacag cggatcctgc
     9181 gcagactgaa gcagggcagt aaacggttgt gcaggcaacg gcatggcgta ccagaatccc
     9241 tgaccgaaat cgcaaccgca ggcctgcagg atcgcatgct gccccggatg ttccacacct
     9301 tcgacaatgg tgcggatacc aagcgtgtgc gccatgacga tcatggcctc caccagcagg
     9361 cgatcctttt tgttatgttc gatgccaatg atgaatgagc ggtcgatctt gaggtactga
     9421 atgtcgaaat ccttgaggta tgacaaggct gaaaatccgg ttccaaaatc atcgatcgcc
     9481 aattcgacac cggaatccct gagctggcga atctgctcga tgaccggatc tgcactgcgc
     9541 aaaagcatgc tttcggtgat ttccagcacc acatgctggc cgatcgagcg cgccagccag
     9601 cacttgtgtt caagttcacg ctcgaactgc agcggcgagc agttgatgct gacctgcagt
     9661 tccgtgccaa aacgctcacg ccagtcctgt tgcgcccgca gcacctgttc caccacccag
     9721 tcaccgagtt cgtgaatgac gcccgactct tccgccagcg gaatgaattg cgtcggcgac
     9781 accatccggc gagacggatg atgccagcgc aacaacgcct cggccttgac gatgcggccg
     9841 ctggccagat caatgatcgg ctggtaatac acttccattt cgccacgttc gagcgcatgg
     9901 cgcagatcat tgcccagatc cattctttca cgcgcatcgc gctgcatcga aggcatgaaa
     9961 caattgtagc ggtttctgcc agccgccttg gcggcataca tcgcctggtc ggcatgcttg
    10021 agcagcgaca tgccatcatc gccgtcacgc gggaacgtgg caatgccaat gctgcccgac
    10081 acatagacct cttcgccgac atcgagcaga tagggctggt tcagtgtggt aagcaactgc
    10141 cccgcaagac cgcgcaccgc atcgtcgtca cccatccccg gcagcaggat gacgaattca
    10201 tcacccccgg gtcgagccac gccatcaccc ccgtctaccg tcatcacaat gcgtcgcccg
    10261 gcctcgcgca gcagcaggtc gcccttggca tgcccgagcg tatcgttgac ttccttgaac
    10321 cggtcaagat cgataagcag caatgtcagc ggaatattgc cacgccggca tttcgccagc
    10381 tcacgttcga ggtggtcgat cagcaggcga cgattgggca aaccggtcaa cgtatcataa
    10441 ttagcctgtc tccagatggt ttcttcacgt gctttttcca cggaaatgtc tgaaaacaaa
    10501 gcgacataac gtattacccg actgtccggc ccatagacgg cgttgattga cacgcgctcg
    10561 gcatagggcg caccatcctt gcgctggccg ggaatctcgc cactccagtg tccagcctca
    10621 agcagttcgc gccatacacg acggtaaaaa acacggtcgc tgaccctggc gcgcagcgcc
    10681 agcgcctgct tgccaagcac ctcgtgctga cggtagccgc tgatacgttc aaacgccgga
    10741 ttgatggaaa gaatatggcc ccgttcgtcg gtgaccagca tgccttcgct gctgttgcga
    10801 tacaccgagg ccgccagcag gatctctcgc tcctgtttgc gtcgcagcga gatatcactg
    10861 aaaaacacca acgatgcaag ttcgccgtcg aaatgaatcg gcaaggcaaa cacctcggcc
    10921 tcgaacacct cgccgttgac ccgtaccagg tgttcttcga gcatctgcac ctgcactccc
    10981 tcgacctggg tgcggtgtgc acgttcggtg acgatggggt ggtaatcctt ctcgacgaaa
    11041 tccagtaaac gcatgtcgac cagccgctgc gggtgttcta cccccagaat gcgtgccgct
    11101 gcgggattac cccacaaaat ccgattgttg cggtgaatca ccagcccatg tggcagcagc
    11161 gcgaccatct ggctgaactc gcgttcgccg tgccgcaacc gctgctccag cgatccggcg
    11221 tcatccaact gcgcatccag ttgcacaggt gcgtactgac ctgattgcca gacgatattg
    11281 attccccggt ccgattcgcc ggtcatgcac cgttcctgcc gcaacaggcg cggctgagca
    11341 tgggccattg caaggcgaaa tcctggtctg gcgaacgacg gaacccgctg cgcacgaaca
    11401 gttgccagcg accgatcacg tggcacagca gcagattggc tgccgctgca ggatctgccg
    11461 tggcatcgcc ctgcacggcg gcgatgcgca acacctgacg caacgtggct tcgatgcgat
    11521 ccagcatctg attgatgcgc gcctgcaacc gcacgttttc atgaaccagc gcatcacctg
    11581 tcagcacccg tgtcataccg gggttcttgc cggcgaaacc aagcagcact gccagcaggg
    11641 cttccagctt gcgctggtcg tcatcctgtc cggcaacgat gcggttgatc agcccgaaca
    11701 acgactgctc gataaattcg atcaggccgt catacatttg cgccttgctg gcgaaatgcc
    11761 ggtaaagcgc tgcctccgac acctgcaggc gcgcggccag cgcagccgtg gtgatttttt
    11821 ccgcttccgg cgcttcgagc atgccagcca gcgtctgcag gatctgcagc cttctttccc
    11881 cttgtaccgc cacaatcact ccccggcaac gcggtgctcc gcgcccattg ccacgacgac
    11941 aacctcagat ttgcggatgc gcgcgttcca gcgtgttata cagtgcgttg agcgcatcca
    12001 gataggcttc ggcagaagct accacgatat cggtatcggc tccctgacca ttcacgatac
    12061 ggccagcctt ggacaggcgc accgttactt ctccctgagc atcggtcccg gtagtgatgc
    12121 tgttgaccga atacaactgc agctcggcct ggctcgatac cagttgttca atggcacgga
    12181 acgtggcatc caccggcccg cttcccgacg cttgcgccga ctgctccgca ccatgcacac
    12241 tgagcacgag actggcacgc ggcacctctc cggtctcgga acagacacgc aacgacacca
    12301 gcttgaacac gtccatgcca tcagcagacg gttcatcgct gaccagcgcg tgcaggtctt
    12361 cgtcaaagat ctcgtgtttc ttgtccgcca gttccttgaa acgggcaaac gccgcattca
    12421 gcgcctcttc cgaatcgaga tcgatgccca gttcggccag tcgggtacgg aacgcgttgc
    12481 gccccgaatg cttgcccagc accatcttgt tcgcgctcca gcccacgtct tcggcacgca
    12541 tgatctcgta agtctcacgg tgcttgagca cgccatcctg gtgaatgcct gactcatgtg
    12601 caaaggcatt cgcgccgacg atcgccttgt tcggttgcac ggcgaaaccg gtgatgccgg
    12661 ccaccagacg cgacgccggc acgatctgcg tggcatcgat gcgcgtggcg caaccgaaca
    12721 cgtcgccacg tgtgcgcacg gccatgacga cttcttcgag cgcggcgttg ccggcacgct
    12781 cacccaggcc attgatcgta cactcgacct ggcgtgcgcc gttgagcaca gcggacagtg
    12841 aattggcgac cgcaagtcca aggtcattgt gacaatgcac ggaaaatacc gcctgatcgg
    12901 aattcgggat ccgctcgcgc agcgtacgaa tcaggtcgcc aaactgcccc ggcaggctgt
    12961 agcccacggt atctggaata ttcagcgtgc gcgcgccggc ggcaatcacc gcttccagca
    13021 cgcgacagag aaaatccgga tcggaacgcc ctgcgtcctc gggagagaat tccacgtcat
    13081 cagtgtgcct gcgtgcccag cccactgcct tgactgcctg ctcgatcacc tgttccggtg
    13141 tcatgcgcag cttcttttcc atatggatcg gactggtggc aatga
//
LOCUS       DAIANL010000070         5758 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1920936, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000070 DAIANL010000000
VERSION     DAIANL010000070.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920936"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1269 a         1851 c         1490 g         1148 t
ORIGIN      
        1 gaaattttgg ttgtataaaa tggtcgtata aagtggaaat acagatcaat acagcaatta
       61 taaccgcaaa atttcaattg caaaagccct gagcccctcg caaaactgga ctaactcatt
      121 gaaaagcgct atgcttccac gtctggcagc gcgcccgtag ctcagatgga tagagtattg
      181 gtttccgaag ccaagggtca caggttcgac tcctgtcggg cgcgccaatg tgatatgaat
      241 ccgggcattc ctgcaggagc gcccgttttc atttctgaca ccgcatttcc agcgcagcgg
      301 tgctgacacg caggccgccg gctctaccgt acatctcgaa aatctactca accagtcaat
      361 ggaaggaacg aaatcggctt cattgcacat cgctcctgcc caatatcccc acaccagcca
      421 acagcttcag tcgtgcggcc tgccattccg ccagggcccg gatacgttcc tgatgtgcat
      481 cagccaaagc cgattcagcg ttcaacatct caagcaaatc agcttcaccg tggtcatagc
      541 gctgccgaga agcagccatc gctgcctgcg ctgcatcaac tagggcggcc gacgccgaaa
      601 gattgccgag cgaagccgac aacaggtccg ccggctggca cgtgccgcgg ttggcaatga
      661 tgacctcggc ctgcccgttc accaccttgt agaacgcaat gccctggtaa ttagtgcgcg
      721 ggtcatttgc tatgttcttg ccaacagcct gtggtggaat ctgtatccac cccgcctgcg
      781 tcatcgccgt caggtcgttc gtgtaattgg ggtcgtacgc ccattgcaag gttgtcgcgc
      841 tggccgcgct gccatagctc aggtcgctcg ccacttgcgc cgcccagatg gtggccgtat
      901 ccagactgct gttgccgcgg ttcgtcgtat cagtcatgac acctctccat caaagggttg
      961 tcctgcacat acctcacggt tggtccacaa aatcccagat catcagacga tgatccgaag
     1021 tcgctgcaaa tctcgaccca ctgggatcta tcgacaagga atccacacca tttgcattca
     1081 atacttccac tgttttaaat gtccgggtat cgataatgtc aatgtcgcca cctgttcctc
     1141 tgccttcatg ccccaccagt aggtacttgc cgtcatcgat gtaacccagc gcatccgtgt
     1201 gtccaacctt gtcggtattg tccagcggcg ccgccagcag ggcatggctc gccacgtcat
     1261 agatgtgcac cgagtccgtg accggagatg aataatagcc aagcactctt ccggtctttg
     1321 tgccaatcgc aatttgccgc ccatccagac tgaacactag cgcagccaag ctcactcctg
     1381 cgcctttata tgccacaatg gtctgatcca gatcgggact cagcagatcc ggcgcatgtc
     1441 cctccacgtc ccagaacaga atccggctgg tatccggaac atcattatgg aagacatggt
     1501 tggcatccat gtagggcttt ggcgctccac gtggaatccc aaaaccacca ctcatgccga
     1561 tcgccagcct gtgcgtaccc ggctcaaacg ccagtcggtc gattccgatt gcccagtaat
     1621 ccgggtggcc caaggcccac acatccgcaa aactcagctc ccggtacaac tgccaggtgt
     1681 ccgtgctgaa cacacagatc atgagactat gtatcccgtt cagatcaacg ccacccgtaa
     1741 cgaaagccag cagtttgctg tccgggctga aagccagaat cccccccccc tgacaacccg
     1801 agcccggctg caaggtcacc ggttgccagt tgcgggtgtc cagtatcgcc aatggcgccg
     1861 cccctccagc ctgacctgtt accaggtagc gtccgtccgg gctccacgcc agaccggaat
     1921 acccgggaga tgtcccatac ttcagcggga tgctcctcac catcctgcgc tgcgccacgt
     1981 cccagatatg gatcttggcg ttcagcacgc cggaatccgc aaggtatttc ccatccgggc
     2041 tccacaccac cgaggtcggg taaaagtcct catccagcac ggcatagggt ttggtgtgcg
     2101 ggctgctgcc ctgcatgccc agccagcctt ccagcaaatt gcccgccagc gccggtccgg
     2161 acagcatggc gcacaaaacc actgcaacca gcaaacccag gcgcagcttc tgctttccaa
     2221 atgacatcag cgcatgcatc catttctccc tgttgttcgc aatgcatatc ccgcgggcat
     2281 gatacaccat ggccataaaa ccaactgcgt ggtcaaggtg taaatgccgt ctgtttttgt
     2341 agcctttgtc ctacagcaca aacgtgaggc cccgaggctg gctgaaccgg aactgcagcc
     2401 tcgcattcat tcaaccgcaa atttgtgaca ggacagtcgt cagccgacag gtattcgcgg
     2461 ccttcccatc acaagactga cgcgccaatc cgatacgaat tcgggcactc caaacaatgg
     2521 tgcccgcttt cattccgtcg tacccatccc gcatctgcac cagaaaagca ctaacaggcc
     2581 gttgaaaaag gtggcgagga cgcccggatg caaggcgcga gaaacgcaac gacgagacat
     2641 atcaaataga taggcgtggt agtgagtatc acagcaacgc cgcagacggg tgccgcagta
     2701 ctttttcaac ggcctgccaa tccttgacag ctgaagcaac cgccgtcaac cgaatcccgg
     2761 ggatccggaa accaacgatg tcagggtttc gccctgtctc cgaagcgccg ccacctgcaa
     2821 cccgccccct gtgcttgaca cgcattttgc ggtaccgcta gactgcccat gcaaccttgt
     2881 gcccgatgca cggttgtacc tctatcctgc agcgcgagac tgcgtgtgtt ttctgctgac
     2941 tgccccatga aactgtgccg ctatggtgcg cctggcaatg aacgtccggc gctgattgac
     3001 ccgcaaggcc ggctgcgcga cctgagcgga cacattgacg acatcgggcc ggagtcgatt
     3061 tcgcccgagg ccctgcaatc cctggccgcc ctgtccaccg cggacctccc gctcgtcaca
     3121 ggaacacccc gtctgggccc gccgctgcgc ggggtgtcca agttcatcgg catcggactc
     3181 aactaccgcg atcacgcccg tgaagcgggg cttcccctcc ctgccgaacc gatcatcttt
     3241 ctcaaggcgc caagctgcat ttccgggcca tacgacccca tccgcaaacc ggcacactca
     3301 acccggctgg actgggaact ggaactgggc gtggtgatcg gcagcacggc tcgccaggtt
     3361 accgaacaac acgcgttgca acatgtggcc ggctactgca tcgtcaacga tgtgtcggac
     3421 cgcggtttcc agtttcagag ctcgcagtgg gacaagggca aaggttgcga cagctttggt
     3481 cccattggcc cgtggctggt cacgcgcgac gaaattgccg atccgcagcg actgaccatg
     3541 tggctggaaa tcaacggcgt gcggcgacag gcaggaaaca cggcagacat gattttttcc
     3601 gtggcacagc tcgtcgccta ttgcagccgc tacatgaccc tgctgcccgg cgacatcatc
     3661 agcaccggca cgccagccgg cgtcggcatg ggccagcagc ccgagcccgt ctggctcgcc
     3721 aacggggatc agacccgcct ctggatcgag ggactcggtg aacagagcca gcgcgtagta
     3781 tccgactgaa cgggcgcaat gcggcgttgt cgcaaggcgc gccaaaacag caggtcaacc
     3841 gatcgcagat ccctttctga cccgcatcat gctggtgcga gtggcgtgca gcatgagcac
     3901 catcctggtg cgcgcaccat aacagggcaa acaggcatat caagcgtatt tttcaccccc
     3961 gcgatcaata caacactatg atttgcaata aaatttaaaa aattccattt gcccgttcac
     4021 tgcacggcat tggctggcat tcttattgct cacgttgccc cgcatacgaa aaacaactgt
     4081 gggtacatca tgcgcaaacg caaaaccagc cagaccctga ccgatcttgc ctatctgtcc
     4141 cgctacatga gcggcctgct gccggatcag aaacgcctga ctgaaataca agccgactac
     4201 gacaatctga ccctgctggg ccaattgctc agtgccggta ccgacatcac cagaatgcgg
     4261 gaaaacttca gtcagctggc ctcgctgctg ctcgatcagc ttgcccggga gcactacaag
     4321 aaagccacgc tgaatctggg ttctggcgcc cgggttgcaa tcgacgtact tacgcgcaac
     4381 ctgttcgagc gtaccgcaga catcggtttt cttgccaccg atgaagacat ccgcaccttc
     4441 gccgaagcgg tggcaaacgg gccatccgca cgcgacgaca gcgaatggcg agcattgctg
     4501 ggtgcgcgct ttgccgagta catcgccaaa tactcggtgt accacaacat catcgtgctg
     4561 tctccggatg gcgaggtgct gttccagcat gacgccgaca atctggtcga ggtcagcacc
     4621 gatccattca tccgcgaagc gctgactaca gatgctggct atgtggaaat attccgtccc
     4681 accgatcttc tgcccggcat tgacagcccg ctggtctacg cctgtcgcat catgtccgcc
     4741 gacaaccacc gaccggtcgg catattgtgt ctgtgtttcc gtttccagga tgaatgccag
     4801 cgcattttcg aaagcctggt ggccgaagac gactggacca tcatcaccct gctggatacg
     4861 tcagggcgca tcatcgccag cagcgacccc tatcagttcc ccatcggggt gcgcatcgaa
     4921 ccggtcagca gcgacgattg ccgtatcatc cgcttcaacg ggcgggaata cctggccacc
     4981 tcacgccacg ctcacggtta tcaggggtat agcgggccgg gctggttcgg ccatgccatg
     5041 gcgccgatca accatgcctt tgaaatgtcc gaagcccgcg aactggatga cgtggcccag
     5101 gatctgctcg actgcgtgct ggaaaccacc acgctgttca gcgaagcgct gcgcaacatt
     5161 ccactgcagg ccgcacgcat ccagcaggaa ctcaatcgcg ccgtctggaa cggtcatatc
     5221 tggctcgctt gcgacgcagc cgcgcacaat gccgagttcg ccaaggtact gctgcgtgaa
     5281 atcggcagca ctggggtacg cacacgcaat gtctacaacg aatccaccac caacctgtac
     5341 aaaaccgtgg tctcgtcggt catgttcgat tgcgctaccc aggctgcgct cgccatcgac
     5401 atcatggacc gcaacctgta tgaacgagcc aacgattgcc gctggtgggc gcttgcacgc
     5461 acattccgtg aagaactttc cagcctgcgg ccgcacgacg acgcgcaaca gcagcagctc
     5521 accacggtat tgcgcacaat caatggcctg tatacggtat atgacaacct gatcctgttc
     5581 gatcgcaccg gcgtggtcgt ggccgtgtcc aattccgcct acagcagcct gatcggccag
     5641 caactcactg aagactgggt gcgcccaacc ctcggactga aaaacacgca gagctattgc
     5701 gtctcgaatt ttgccgcgac gcaactgtac gccggccgtc acacctatgt ttattctg
//
LOCUS       DAIANL010000071        23406 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1520824, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000071 DAIANL010000000
VERSION     DAIANL010000071.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 23406)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23406)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23406
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520824"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4797 a         7476 c         6542 g         4591 t
ORIGIN      
        1 acccgtcgat caggtagcct ggggcgccgg cgcaaccacc cctcagctgg cgcaattcat
       61 gccattgcgc aacctgaaac tggaatggat cgaacaggca ccttatatcg cggcacgtaa
      121 tggatcgatg ctggcgcagc gcgtactcga cgaactggaa cgcggcagtc accggccggc
      181 gactgacggc acatcactgc tggtcgcaat gatcggacat gacaccaaca tcgccaacct
      241 gtcaggcatg ctgcgcttgc actggcatga tgcgcgccag cctgatgata tagcacccgg
      301 caccacgctg gcgttcgaat tgctacgcga tacacacagc ggggagtatt tcgtgcgcac
      361 acgactgttc atgcagactg ccgaccagtt gcgcaccgct gctccgctcg atacggagca
      421 cccacccatc gtgcaggagc tggcggttcc gggatgcgtc ggggaacacg acgacctgtg
      481 cccgctcgct gccttcagcg cgctgctgcg ctcgcgcatc gacccgcagt gcgtgaccga
      541 caaccccgaa ctcacgcgga ctagcacata ctgacgctgt gcgcaaccgg cccggttcat
      601 gacacgaagg catgccaaac ggcgacacgc catccaccgt gcgacggaca gcgagaagaa
      661 gatgcctgcg gattatttct cgtcgatcca ggccatctgg atggcttcga gaatcttttc
      721 gctggaacgc tgcggatcat cggaaaattc cggcagcgcc agcacccagg cgtgcagatc
      781 ggtgaaacgg atgtaacgcg gatcgacgtc gggatgagtc tccaccagct cgatcgcgat
      841 atccagggta tcgtgccagg tcagcatgtc agtgtccttc cttgaccatg ttgatactgt
      901 acctggggat ctcgatcacc aggtcggtat cgccgacgac tgcctgacac gacaggcgcg
      961 aatgtggttc cagcccccat gccttgtcga gcatgtcgtc ctcaagctcg tcggacggtt
     1021 cgagcgaagc gaagccttca cgcacgatca catgacaggt ggtacaggca caggattttt
     1081 cgcaggcgtg ttcgatctcg atatcgtgtt cgagcaacac atcgcagatg ctctcgcccg
     1141 acggagcatc gatgacggcc ccttccgggc acagggttgc gtgaggcaat acgatgagtt
     1201 gaggcatgat caatcctggt aaggttcgaa gtcgtcgccg gttcagccga tgctgtcgag
     1261 acgctggccc gccaacgcct gcctgacacg tgcgtccatg cgccgtccgg caaattcatg
     1321 agtggtctgg ttgagctgat ctgcagcagc cttgattgct gcgctgtcac tcgatgcaag
     1381 cagctcttcc agctggtgca cgcccgcgag gattccggca tattcttcgc cgcgcagcag
     1441 atcggcaccg tcttcgtcca gcgccgcctg ggttgcctcg cacagccgtt gtgcttccac
     1501 gcgcgcttcc tgcaatgtgc gcgcttccat gtcctcacgc gcgtggctgt atgccgattg
     1561 caacatgcgg ctgatctcgt cttcggccag cccgtaggtc ggacgcaccg tcaccgatgc
     1621 ctgcacgccg cttttgagtt cctgtgccga gaccgaaagc agtccgtcgg catcaacctg
     1681 cagcgtcacg cgaataggcg cggcgccggc caccatgggc ggaatgccgc gcagttcgaa
     1741 acgggccagc gaacggcagt cactgaccag ttcgcgttcg ccttgcacca cgtggatgct
     1801 catggcggtc tgcccgtcgc ggtaagtggt gaattcctgc gcgcgcgctg caggcagcgt
     1861 ggtgttgcgc ggtatgatct tctctgccag tccgcccatg gtctcgatgc cgagcgacag
     1921 cggggtgacg tcgagcaaca gccagtcttc gtgttcagca cggtttccgg ccagcacatt
     1981 ggcctggatg gcggctccaa gcgccaccac cttgtccgga tccaggttgg tcagcggcgt
     2041 ctggccaaac agcgccgcca ctgccgactg gatgcagggc atgcgcgtcg acccacccac
     2101 catgaccacg cctcgcacgt cctcgacgcc cagtccggca tcacgcagcg ccttgcgcac
     2161 cggcaccaat gtcttgttga ccagcgcagc cgtcatctcg ttgaactgcg cagccgtcaa
     2221 tgtcagatcg atggtcagtc cggacgacac cacagcggtg atatcgacct cggtatgctc
     2281 ggacagcgct tccttggccg cgcgcgcctt tgacagcagc aggcgtgtat cttcagcgct
     2341 cagatcggga aatcgcgcct gttcgagcat ccagcagaac aggcgctggt cgaaatcgtc
     2401 accccccagt gcggaatcgc cattggtggc cagcacctcg aacacaccgc gcgacaggcg
     2461 cagaatggaa atgtcgaagg tgccgccccc cagatcataa acggcgtaca caccttcgct
     2521 ggcgttatcc agcccgtagg caatggccgc ggcagtcggt tcgttgagca ggcgcagcac
     2581 ttcgagcccg gccagttgcg ccgcatcctt ggtcgcctgg cgctgcgcat cgtcgaaata
     2641 ggccggcacc gtaatcacag cgccgaccaa ttcaccaccg agcgcatgtt cggcacgttc
     2701 gcgcagcaca cgcagaatgt cggccgacac ctccaccggg ctcttcacac cggcggcagt
     2761 gcgcaactgc accatgcctg gcgcatcgac gaagcgatag gcaaaactcg acgcatcgtg
     2821 aatgtcactc agcgcacgcc ccatgaagcg tttgaccgag ctgatggtat tatgcggatc
     2881 acgggtttgc gccagatggg cggcatgacc gaccaccggc acctctcccg cctgatagcg
     2941 tacgaccgac ggcagcaatg cgcggccatc ttcgtcattg agcaccagcg ccacgccatt
     3001 gcgtacactg gccaccagcg aattggtcgt gcccaggtca atgccgaccg ccagcctgtg
     3061 ctggtgaggg gcggtactca tgccgggttc ggcgatttgc agcaatgcca tgattcaact
     3121 ttccagttcc gaccagagcg tatcgatttc ttcctgaatc ttttccacga acttgagctg
     3181 acgcacggtc tgtgctgccg cctgttcgtc gtgcaacctg tcaagctcat ccgccagcct
     3241 ggcttgccaa cgcttgccgg ccgagcggat ctcatcttcg atgcgttgca gcgcatcact
     3301 gttcctgttc gccctggcct cgccaatcgc ctcgcgccac tcgatctgct gcatgaggaa
     3361 agctgccggc aaggccgcat gccccggcgc aaacacattc acgccctgta atgacaacag
     3421 gtaagccgcc cggctgatcg gggagcgcaa tacctgccag gcctcgttga cgcgcgtcga
     3481 ccattgcatg gacaagcgct gctcggcctc tggaaggtgc gcgaacttgt ccggatgcac
     3541 ctgcgcctgc agcgaacggt atgcctgctc gagtgccgga agatccagct ggaacgtcgc
     3601 aggcaggcca aacagttcga aatggctggc tttgaaatcc ggcaacatca gacgttgaaa
     3661 ctttctccgc aaccgcagga attcttcacg ttcgggttgt tgaagcgaaa cccttcgttc
     3721 agtccttcgc gagtaaaatc gagctccatg ccgtccagat agggcagact cttcggatca
     3781 accagcacgg tcacaccgtg gtcaacgaaa gtcacgtcgt cgtcctcgat gcggtcggca
     3841 aattccagct tgtaggccat gcccgagcag ccgctggtac gcacgcccag ccgcaatccc
     3901 acgcccttgc cgcgcttggc aaggaattcg ctcacatggc gcgctgccgt ttcggtcaat
     3961 gtgattccca tgattatctc acgcctgttc cgattcgacc tggacaccgt gcttgacgcg
     4021 gtagtcctcg actgccgcct tgatggcgtc ttcagccagg atcgaacagt ggatcttcac
     4081 cggcggcaac gcgagctctt cggcaatggc ggtgttcctg atctccagcg cctgttccag
     4141 cgttttgccc ttgacccatt cggtcaccag tgagctggat gcaatggccg aaccgcagcc
     4201 ataagtcttg aaccgcgcat cttcgatcac gccgccatcg ttgacgcgga tctgcagtcg
     4261 catcacgtcg ccgcaagccg gcgcaccaac catgccggta ccgacagctt cgtcatcggc
     4321 tgcaaacgaa ccgacgttgc gcgggttttc ataatggtcc agcaccttgt cactgtatgc
     4381 catgatagtt ctccttaaac cagtttatgg cgtcacaagc atcctgtgcc tgttcctgaa
     4441 tccttgcctg ccaccttgcc tgcggcgcac cagtcaatgc gccgcccact tcacgctgtt
     4501 cagatcgatg ccttctttgt acatatccca caaaggagac aattcacgca attttccgat
     4561 tttttggtgc aacagcgcaa cggtataatc aatatcctgt tcggtggtga accgcccgat
     4621 ggaaaaacgg atcgagctgt gcgccagttc gtcggaacgt cccaacgcac gcagcacgta
     4681 ggatggctcc aggctggccg aagtgcatgc cgaaccgctg gaaacagcca ggtccttcac
     4741 cgccatcagc aacgattcgc cctcgacgta gttgaagctc aggttgaggt tgtgcggcac
     4801 gcggtgcacc atgtcgccat tgacgtgcac ttcctcgatg tccgacagcc ctgccagcag
     4861 gcggtcgcgc aggcggcgga tgcgcgcatt ctcgatggcc atctcttcac gcgcgatgcg
     4921 gaacgcctcg cccatgccca cgatctggtg cgttgccagc gtgcccgaac gcaagccgcg
     4981 ctcgtgtccg ccgccgtgca tctgcgcttc cagccgtacc cgcggcttgc gccgcacgta
     5041 cagcgcaccg atgcccttgg gcccataggt cttgtgtgcc gagaacgaca tcaggtccac
     5101 cttgagcttg ctcaggtcga tgtccacctt gcccgtggcc tgcgcagcgt ccacatggaa
     5161 aatcaccccg tgttcacggc agatctcgcc cagttcggca atcggctgga tcacgccgat
     5221 ctcgttgttg acggacatca ccgatgccac gatcgtgtcc gggcgcagcg cggcacggaa
     5281 cacttcgagg tcgagcagcc cgttcgactg cggcgccaga taagtcacct cgaacccttc
     5341 gcgttccagc tcacgcagcg tgtcaagcgt ggccttgtgt tcggtctgca ccgacaccag
     5401 atgcttgccg cgccccttgt agaagtgcgc cgcgcccttg accgccaggt tgatcgattc
     5461 ggtcgcaccc gaagtccaca cgatttcctt ggcatcggca cccaccagcg ccgccacctg
     5521 ttcgcgcgca gtttccaccg catcgtccgc ctgccagcca aaggcatgcg accggctggc
     5581 cgggttgccg aactgttcgg tcaggtacgg aatcatcttc tgcgccacgc gcgggtcaac
     5641 cggtgtggtg gcggcatagt ccagataaat cggcgatttg atcatcagtc actccatcat
     5701 tcttacaagg gggtcagagc acggccggaa ccacgccatg cgacgggcgc tcgaacacca
     5761 ccgcactcgc acagtcatca tgatccttgg cccgctgcag ctgaaccagt gactgcaggg
     5821 tcacgccctg caggtattcc tggatgcact gattgaggct cgtccacaac tcatgcgtca
     5881 tgcagggctt gtcatccatg caattctgca taccggcaca ctgggtggca tccatgggct
     5941 cgtccaccgc ctgaatgatc tgcgccacgg tgatcgcttc cagtggacgt gcgacgagat
     6001 aacctccacc cggcccgcgc acgctctcca ccagctcgcg gcggcgcagt ttgccaaaca
     6061 gctgttccag gtaggacagc gaaatcttct gtcgttcgct gatgcccgcc agcgcaaccg
     6121 ggccttcttc atgccgcagg gctagatcga tcatggcagt cacggcgaac cgccccttgg
     6181 ttgtcagtcg catgactttt ctccggcaaa ttctgatgcg atgagcatat aatacctgaa
     6241 tattttagtc aactattttg ttcaggtatg cggcgtcgaa atcgtcacgc cgcggttcgc
     6301 tggcagtaac gcctatctgc tgcagttgcg ccatgatcca ttccagccgg ccatccaccg
     6361 tggccgcatg atcgatgagg ccgtgcatcg ccttgaccat cgggtcattc atgtcagcgc
     6421 tgatggcata ggcagaaaag cccagcttgc gcaccgtggt ggcctgttct gcctttgatg
     6481 tcacgatgcg cgccggaatc ccgaccgccg tggcacccgc gggcacgtcc ttgaccacca
     6541 cggcattgga acccaccttg gcgccatcgc ctatcatgat cggtccaagt atcttggctc
     6601 cggcacccac caccaccccg ctgccgagtg tcgggtgacg tttgccttca ttccaggagg
     6661 tgccgcccag cgtgacctgg tgatagagtg tgcaatcatc ccccaccacc gccgtttcgc
     6721 cgatcaccac ccccatgcca tgatcgatga acacacgacg cccgatctgt gccccggggt
     6781 gaatttcgat cccggttccc cagcgcgccc accaggcaat ccacctggcc agccacttga
     6841 gacgcagacg ccacatcgac gctgcaagac gatgcagcag aatggcatgg atgccggggt
     6901 aggtggtcag cacctcgaac acggaacgcg cggccggatc ccggtcgaat actaccgcca
     6961 catcttcgcg caattgcctg aacacgatac cctcgatctg gttccagcag ccagtatgca
     7021 atacccgaca agattattca acgatatcgt tgtcctgaat tccccctccc cccggccggg
     7081 gattacggat gcgtttgagt atccccatga gaatattgaa ttcttcatgt tcgatcctgg
     7141 tgcgcgaaaa cagccgccgc aagcgcgtca tcaaccgttt cggcatgagc ggatcgagaa
     7201 agccgatgcc gatcagcgtg ctctccagct cccggtagaa acgttcgagg tcatcgtgcc
     7261 gcgccagcgg aaatggcacc tcgtccggcg cccgcgccga taccgatgcg acacgcaatt
     7321 catatgccag aatctgcacc gccgctgcga gattgagcga actgtaatcc gggttggctg
     7381 gaatgcgcac cagtagctgg cattggtcga gctcttcgtt gctcaacccg gacatctcgg
     7441 tgccaaacag caaggcgacg ggcgcgcgcc gggcatgcgc ggccagcagc cctgatgcct
     7501 cgcgtgcatc gatcatgcga tgcgacaggt cacgcggtcg tgccgtgcaa cctgccacca
     7561 gttccgcccc gatgagcgca tcttcgagac gcgtcaccac ctgcgctgca gccagcacgt
     7621 cgtcggcgcc agaagccatg gcctgcgcag ccgcatcggg aaaggttttc ggccgcacca
     7681 gacgcagatc gcccagcccc atggtcttca tggcccgcgc cgcagcgccg atatttccgg
     7741 gatgactggt ctggcacaac acgatgcgca cccgttgcaa tgacatcgcg tctcctgcct
     7801 gtggggcgtc catcatgatt ctggggtaaa atatccgtgt ttactgctca ttactcgcca
     7861 tttcgccatg catcccatgc ttaccacggc catcaaggcc gctcgccgcg ccgcctcgat
     7921 catcaaccgc gcatcactcg acctcgatgc actcagcgtg cagagcaagc agcacaacga
     7981 cttcgtcagt gaggtagacc gggcggcaga agccacgatc atcgaaaccc tgctcgatgc
     8041 ctacccgcaa catgccattc tagcagaaga gagcggccac actggccaat cggaatatca
     8101 gtggatcatc gatccgctcg acggcaccac caatttcctg cacgggtttc cccagtacgc
     8161 agtatcaatc gcgctggccc acaacggcca gctcgaccag gcggtcgtct acgacccgac
     8221 gcgcaatgaa ctgttcgtcg cttcgcgcgg caagggcgcc tacctcaatg accggcgtat
     8281 ccgcgtgtcg aaacgcgccc gactggccga agcgctggtc ggcaccggct tcccgttccg
     8341 cgatttcagc cacctcgacc cctatatggc catgctgcgc gacatgctgc agaagactgc
     8401 aggcgtgcgc cggccgggct cggccgcgct ggatcttgcc gctgtcgccg ccgggcggct
     8461 ggatggcttc tgggaaatcg gcctgtcgaa atgggatatt gccgccggct gccttctgat
     8521 tcaggaatct ggcggtctgg ttggtgactt cgagggcaat gacagttaca tcgacaccgg
     8581 caacatcgtc gccggcaacc ccaaggtatt cgcgcagatc ctgcacgcac tgacgccgca
     8641 cctgacgcct gcgctgcgca gttgaaaatc cgggcaccca gtgcaccagc ggggagaatt
     8701 tagggtaaaa ttcgcgtttt tccgcacaaa acctattttt ctcaccttcc actcatggct
     8761 ctcatagtcc agaagtacgg cggaacttcc gtcgccaacg tcgagcgcat caagcatgtc
     8821 gcagaacggg ttgcaaaatt caagatgctc ggccaccagg tggtggtagt cctctcggca
     8881 atgagcgggg aaacaaaccg cctgattgcc ttggccaggg aagtccagtc cgaacccgac
     8941 ccgcgcgaac tcgacgtact ggtctcgacc ggcgagcaag tcaccatcgc gctgctggcc
     9001 atggcactga aagatctcgg gctcaaggcc cgaagctaca ccggtagcca ggtgcgcatc
     9061 ctgaccgaca atgccttcac caaagcgcgc atcctgcaga tcgatgaagc ggcgctgcgt
     9121 gccgatctcg atgccggacg ggtggtggtg gtcgcaggct tccagggtat ggatgccgcc
     9181 ggcaacatca cgacgctcgg gcgaggcggt tcggatacca caggagtggc acttgccgcc
     9241 gccctgcatg cagacgaatg ccagatctac accgacgtcg atggcgtcta taccaccgac
     9301 ccgcgcatcg taccgcaggc acgacgactg gaaaccatca ccttcgaaga aatgctggaa
     9361 atggccagcc tcggttccaa ggtgctgcaa atccgctcgg ttgaatttgc cggaaaatac
     9421 aaagtcaaac tgcgcgtgct ctccagcttt caggatgaag gcgaaggcac gctgatcact
     9481 ttcgaggaag aaaagcatat ggaagaggcg atcatctcgg gtatcgcatt caaccgcgac
     9541 gaagcaaaga tcaccgtgca gggcgtcccg gacaccccgg gcgtggcatt ccagattctt
     9601 ggccccgtgg cggatgccaa tatcgacgtc gacatgatca tccagaacac cggcgcgaac
     9661 aataccaccg acttcacctt caccgtgcac cgcaacgact acgcccgaac catcgcattg
     9721 ctcggcaagc tgcagccaca aatcggcgcg cgcgaaatca tgggtgacga acacatcgcc
     9781 aaggtatcca tcatcggtgt gggcatgcgc tcgcacgtcg gcatcgccag caccatgttc
     9841 cgcaccctgg cccaggagaa catcaacatc cgcatgattt ccacctcgga aatcaagatc
     9901 tcggtagtca tcgatgaaaa atacctggaa cttgccgtgc gcgtgctgca tgacgccttt
     9961 gaactggaaa aggcgcccgc ggtttgactt aaccccgtca ttgggctagt attacgcgct
    10021 gcccacgggc atgcaagttc cgctgcaata cggagagatg gccgagtggt cgaaggtact
    10081 cccctgctaa gggagcgtcg gatcaaaaat ctgaccgagg gttcgaatcc ctctctctcc
    10141 gccagatgca ataaatcaag ccactgatta cagtggcttt tttatttcat aacaaaccaa
    10201 acgccaataa gaccgtaaac cgaacgtctc atcgagtgcc atatagtccc accacgtttc
    10261 acccagacac acacagttac cgagtaaaaa tcggaggaaa acgaaaccgg gaaatcatgg
    10321 caatcgacaa acctgccgat gaggcaatcc gccgcttgcc gtcaagcgca acggcaaagc
    10381 ggtatggtga tgatggtaac ttattcttgc aggttgagcc gtcgggtgcg atgcgctggc
    10441 caacaaactg ccaccacaga acagtccgaa gctactttcg ccgctccgct ggcacaggct
    10501 tcaaaccagc accctgtttg acattcccac tggacatttc aatgttaatt catataatga
    10561 acgataattt gtttattttt taaattaaaa gcgccgccca tgctccataa ttatgtattt
    10621 tccaacttcc agtcgtttcg ggagcggact gaggtcgatc tgaccctgaa ccgcaaggta
    10681 cctctgacga actggatgga cgaggcggct acgagcgagc gtgtgtcgaa gttgatggcc
    10741 attgtcggcc cgaatggcag cggcaagaca gccttgctca aaccaatggc ctttttgagc
    10801 tggtttgtca ctcattcatt tcagagccaa ccggatgccg gcatcccgct gacaccgcac
    10861 gccgcagccc agcacgagcc cagcgaattc gaatgcatgc tggattttga cggcaagctg
    10921 tggcgctatg tgctgcgttg cacgccggag cgagtgctcc atgaggcgct ctaccagaga
    10981 cgcgagcgct ttggctatgt cttcgtgcgt gattgggacg catcaaccaa cgcttatgtc
    11041 gtgaagcagc aggacttcgg cctggcacca caggaggcgc gcaaggtgcg cccgaatgtc
    11101 tcgctgattt catgggcggc gcagttcggc gtgccgctgg cgacgcgcat ggtcttgcct
    11161 tatgtcatta ccaacgtcaa cgtgctgggc aggatgccga taggggcgct agacgtatcg
    11221 tccgccgcgc agcacttctc ggttcatccc aatcagcgtg agcggatgaa tcaactgctg
    11281 gcggcatggg atttggggct gtctgacgtc gagttgcacg agattcccac caataacccg
    11341 caaaagcccg atgagaaaat atggatacct ttcggcaaac acaaagatcg cgagactgag
    11401 ttcagcttac ctttttccct ggaatccagc ggcacgcagg gcgcattcgt gctgctgtcg
    11461 cgcctgctga acgccttgga gatcgggggg cttgcgatca tagacgagtt cgagaatgat
    11521 ctacacccgc acatgctgga acccatactc gatttgttcg ccaacccgtc caccaacccg
    11581 catcaggcgc aactcctgtt tacttgccat gctgttgaag tgttgaacct catccacaaa
    11641 tctcaggtga tgctggtgca gaaggattct aactgcgaaa gttcggcaat acgcctggat
    11701 gcggtggcag gcatccgcaa cgacgataat ttttacgcaa aatatatggc aggtgcctat
    11761 ggcgctgtgc cgcaaatctg aacggcacgg atggttatct ggacagcaca gcgcactgta
    11821 ctcatcgtcg gagaaggcgc cgatgaagtg gcgtttctca accatgtaaa acacttgtat
    11881 gtgccgcgtg gctgcggctt atcggtaaag atcaaaaacg cacaaggcaa gggagccagg
    11941 catgtggtca actggactgc acggcaaatg gacattggtg tttatgacaa ggttgccgcc
    12001 ctgttggaca ctgatgccga ctggtcgcct gccgttaaca aaaccgccag ggaaagaaag
    12061 attcaggtgc tcacatcgga accctgcttt gaggcactga tgctgcgtgt attgggggaa
    12121 aaccctgaca gcaatgcgtc cgcactgaaa aggcagttcg cccccttcgt taataacgat
    12181 gcaacgcaaa gccggaacta cgccaaccat ttcagcactg aacgcctgca agcgggttgc
    12241 ttgcatgagc cgacgatcaa ggcactgctt aatctcatta aaagttgaaa cagatcgtta
    12301 ccacgaaccg ctcaaccaga tgcgcttcgt gcaggacggg attaccttgt cgcatcgatg
    12361 taccgcgctt gatagcacct cgcttctcaa cattttcacc aggcagatca gttaagccga
    12421 tcaacggcca cgacattcac tgcccctgaa tcaccctgtc cagcatctgt tccatctgca
    12481 gccgcccttc cggcgtggtc atcagcatca gcgaagcgcc gggattgatc atgtgatcgt
    12541 cgtcggggtt gaatacccat gcaccccgtt catgcgtggc catcaacaga taatcgcggg
    12601 tgttgccgcg cagtaccccc agcgcggtcg gcggaaactg atccggcacg agcacctctt
    12661 ccatgcgcaa cccttcgtca gtctgcatca tgcgatccat gaagttgacc acatgcggac
    12721 gcaacatggc tgacgcaata cgcatgccgc cagtaaaatc aggcgacacg atctggtctg
    12781 cacctacccg gcgcgccttg ttctcattac ggatgtcgtg caggcgcacc accacacgcg
    12841 ccttggggtt gagcagcttg accgacagcg caatcatcag gttgtggctg tcgtcggacg
    12901 tcagcgcgaa cacacccgcc gcctgcatga ccccggcacg acgcagggca tcgtcgtcag
    12961 ctgcgtcatc gagcagatag taggtctgcg gattgcgttc gagccaggtg tcgatgacgc
    13021 tacggttgct ttcgatcacc acgaacggcc gccgggtctt gagcagttcg ctggccacgt
    13081 tggaaccgac gcgccccact ccacacacga tgtaatgccc ggcaagggcc gcaatttcct
    13141 tttccatgcg tttctttctg agtccggcat tcagatcggc gtcgaccagc agcgccacca
    13201 atcgggagaa caggtagctg agcgtgccga tgccgaacac ggcgatcaaa acagtaaaca
    13261 gcctgcccag cggatgatgg ttcaggtcca ccacctcggt aaaaccgatc gtcgagatgg
    13321 tgatgaaggt catgtagaaa ctgtcgaccc aggtcgccgt ggggcggcca atgtaatggt
    13381 agcccagggt accggtcgca gtcaccagca acagtagcac caccccggct gccagcccgt
    13441 aaaagtgccc actttgcaat atgcgtttca atccggttgc ccccgcagca gtacgtacgt
    13501 gacggccgtc atggccatca cgcgacacgc ctcattttat caggctgttg caggctgatg
    13561 cggatccgtt ggcagcggcg cagaacctcc gtcacaccct ggtcagcggg agtgacgagg
    13621 cccgttcagg gcagcatgcg gtcacgtgcc ctgaaatagc tgcgtgcgtg cgccaccagc
    13681 tgggcatcgg tcggatgatc gacggtttcc acgcctatca cgtgttccat tttctgcgga
    13741 tcgtgctgcg caaggtattt gatgaagacc agtttggcgg tcgagggccc gacaacgagt
    13801 atctcgtgcg cgtcgccgat ggcctgggcg atgtcgcggt aatagtgctg atcttcggcc
    13861 aggcggccat cgcctatgat gccgcgcttg atgtgcagtc gcggtcggcc ttctgcggga
    13921 tggacgatgg atgtttccac atcctccggg ttgacatgca tgacatgcgc ctccgaatga
    13981 tccagccaga ccacaacatg atagtgtgcc atgacattcc tcccgcgaaa actgcaactg
    14041 acactggtat tatgcggctc agcgaccaaa tttcaagtcc ctcaaccggt catcaggcgc
    14101 accgcgcgat tcagacggtc gccagccgca catcaaagtg cggaaaacgg cttgccagcg
    14161 cgcccgcccc gacatcatcc agataaccgg cttccatatc gggaaacgtt tctgcgctgc
    14221 cttctgcacg gtactcctgg agcacataat tgtttgcgcc gcgcgcagcc agccattcgc
    14281 cgattttctc cagcgaagcg cgattcagga gagacgaatg caccgtggtg cggaattcgc
    14341 actgaatacc gctgtcgagc agcagtcgtg tgctggcgag agccttgaga ccgctgccgg
    14401 ccaccccggt cacccggtcg tactcatccc acggcgcctt gatgtccatg ccgacccaat
    14461 cgagcagcgg cagcaggcgc cgcaatctcc cgggattggt gccggccgta tgcaggccca
    14521 ccttgaatcc cagcgcacgc acggcgcgaa tggccgccac cagatctttc tgcagtgtcg
    14581 gctcgccgcc gctgaacacc acggcatcga gcagcccacg acgacgttcg agcatgttgt
    14641 gtacatcggt ccaggtcact gcagccgcac tgtgtgccgg tcgcaggtgc gaattgtgac
    14701 agtagccaca gctccaggaa cagccctgac agaacacgac tgccgacaac aacccgggat
    14761 agtcgacact ggaatatggc accatgcctc cgatacgcaa catgctattt ctccgatcag
    14821 tttccgccct gcatcagggc cggttcacga aacagccgcc gttcggcgtg ctcacccttc
    14881 ttgcccacat tgaatgaggc gaccggacga tggtagccca tcacacgcgt ccagacctcg
    14941 cacggctggc gctcgaaatc ctcaagcgtg atgacgtgtt ccggcaaatt cgttctaact
    15001 gtactcatct tgaccactcc ttgtcaaaca attatcaaca atccacccgc acgttgctgc
    15061 cgggcggtca cgccatcatc agtgcacgtt tctgcgcgat cttttccgca tcgcatttcg
    15121 gacagaactc gtattcgccc gccagatagc catgtttcgg gcagatggaa aacaccggcg
    15181 tcacggtgag atagggcaat gaaaaccgtt ccagcgaacg tttcacaagc cgcttgcacg
    15241 cctcggggct cgacacccgt tcgcgcatgt acaggtgcag caccgtgccg ccggtgtatt
    15301 tcttctgcag gggttcctgg cgttccagcg cctcgaacgg atcatcggtg aaaccgaccg
    15361 gcaactgcga cgagttggtg taataaggtt tatccgtcgt acctgcctgc aggatgtcgg
    15421 gataacgctt gagatcctcg cgtgcaaacc ggtaggtggt gccttcggcc ggcgtggctt
    15481 cgaggttata catatgcccc gtctcgtcct ggaaatgcag caaccgcgcc cggacatggt
    15541 cgagcaaccg catggcaaag gcatgccccc attcggtggt gatgtcttcg gtatcgccgg
    15601 taaaattgcg gatcatctca ttgatgccat tgacccccag cgtagagaaa tgattgcgga
    15661 aggtgcccag atagcgttgg gtatagggga acaggcccgc atccatgtgc cgctgcaata
    15721 ccttgcgctt gatttccagg ctattctttc ccagttcgag caatgcgtcg aggcgctgca
    15781 gcatgcccgc ctcatcgccg cgatagacgt agcccaaccg ggcgcagtta accgtgacca
    15841 ctccgaccga ccctgtttgc tcggcgctgc cgaacaggcc attgccgcgc ttgagcagtt
    15901 cacgcaagtc cagctgcaaa cggcagcaca tggaccgcac catattgggc tgcaactcgg
    15961 aattgatgaa attctggaaa tacggcaacc cgtacttggc tgtcatggcg aacagccgcg
    16021 ccgcattctc gctttcccaa gggaattccg gcgtgatgtt gtaggttgga atgggaaagg
    16081 taaacacacg ccccttggca tcaccggccg tcatcacctc gatgtaggcc tggttgatca
    16141 tgtccatttc cacctgcagg tcgccatagg tgaacggcat ttcctcgccg gcgatcaccg
    16201 ggatgcggtc gcggaggtcc tgcgggcagg tccagtcaaa cgtcagattg gtaaacggtg
    16261 tctgcgtacc ccagcgcgat ggcacgttga ggttgtaaat cagctcctgg atggcctgcc
    16321 gcacttcggc ataggacaga ttatccttgc gtatataggg cgccagatag gtatcaaacg
    16381 aactgaacgc ctgggctccg gcccattcgt tctgcagcgt gccaagaaag ttgacgatct
    16441 gtcctaccac actggacaaa tgtctgggcg ggctggcctc gaccttgccc ggcacgccgt
    16501 taaggccttc gttgagcagt gtgcgcagcg accagccggc acagtaacct gccagcatgt
    16561 ccaggtcgtg cacatgaata tcgccttcgc gatgcgcctg accgacttca ggcggataga
    16621 catggttgag ccagtaattg gcgaccacct tgccggacac attgagaatc aatccgccta
    16681 ggctataccc ttgattggca ttggcgttca cgcgccagtc ctgctgttca agatattcgt
    16741 tgatcgatgc agccacatcc accaccgtgc gccggtcctg acgcagcttg tgatgctgct
    16801 cgcgatacac gatataggca cgcgcagtgc gcagatgatt ggcggatatc agcacctgct
    16861 cgaccacatc ctgtatctgc tcgattccgg gagcctcccc gtgaaatctg tggagcagca
    16921 ccttggacac ctgcgccgtc agcagatcgg cttcaccgct gtcaaattcc ccgctcgact
    16981 gaccggcgcg agcgatggca gaacggatct tgcctgcatc gaacggcgcg cgggtgccgt
    17041 cacgcttgag caccgctgtc ggcagcaaca gtggctgtgt ctggcttgaa gtctgtatct
    17101 gttgctgttc ctggctaaat gccggcatgt tctctcctcg tagcaatatc gatgtaccac
    17161 ccgtgaatgc gcgccttaac aaacactaca tatcggtttt atgaactgca aatatacatc
    17221 atattgttat ctggtcaagg atagttacag atatttttat ctgtattgtt taaacgaaac
    17281 accgcgcagg gcagttgcag tccctgcctc agcttctgtc gccctgccgc gtgccgcgct
    17341 ggcgcagttc ggcttcgatg ccgttgagta cccgccactt gctggcgcac caatcgggcg
    17401 caagcaggat atccatcgga gccgtagccg tgacacgatg gtagatcacg tcaggcggcg
    17461 tacgctcgat catgtcggca gcaatccgca ggtattccgg cagttccagc ggctgatacg
    17521 ctcccctgcg ccactgctgc gccagcacgg tatgacgcac aacatgcaag ggatgcagtt
    17581 tcaacccatc aacgcccagg tccagcacgg tatccagcgt ggcgtggcag tgatactcgc
    17641 tttcgcctgg cagaccgacg atcagatggg tacacagcgg cagcccgatg cgccgtgccg
    17701 cacgcgccgc aaggcggtat tcctccagcg aatgaccgcg acgcacacgc gccagcgtgg
    17761 tatcgaacgc tgactgcagc cccagttcca gccacaccag ccaaccttcg tcgcgcaatc
    17821 gcgcgagcag ttcgagtacc tgttgcggta cgcaatccgg gcgtgtgccc accgacagtc
    17881 caaccacgcc aggttgcgcc agcgccgtac ggtacatggt ttcgagctgc gtcacatcgg
    17941 cgtaggtgtt ggtatacgcc tgaaaatacg ccacatagtg tcgcgcgcca gtacggcgca
    18001 cgatcactgc cgaggctttg gcgatctgtg tctgcagcga cagacggctg tcggcatccg
    18061 gactgaacga agcattattg cagaacgtac atccacccgt tcccttgctg ccgtcacggt
    18121 tcgggcacgt cagcccggca tccagcgcaa tcttgtgaat gcgccgaccg tgatggcgga
    18181 gccagaattg ccccagggtg ttgactctgt cagaaagtat catggccata ccctgccccg
    18241 ctgccgccat gcaacactca ggcaacagcc gctgtggcgg ggctggatgt ttaaataaaa
    18301 agatcgtatt atcttacaac tattccgctt catccagaac aggtttaaac atgtcagaat
    18361 cccacactcc cgcttcactg cgactcaaga ccggattggc cgaaatgctc aagggaggcg
    18421 tcatcatgga tgtcaccgat gcccgccagg ctgaaatcgc cgaacgttcc ggcgccgtcg
    18481 ccgtgatggc gctcgagcgc gttccggcgc agatccgcgc agaaggcggc gtagcgcgca
    18541 tggcctcgcc ggtcaagatc cgcgaaatca tgtcggcggt gtccatcccc gtcatggcaa
    18601 agtgccgcat cgggcatttt gccgaagcgc agatcctgca acagctcggt gttgattaca
    18661 tagacgaatc tgaagtactt acacccgccg atgaacacca tcatgtcaac aagcacgcct
    18721 ttgacatccc attcgtctgc ggtgcgcgca acctcggcga ggcactgcgg cgcatcgccg
    18781 aaggtgcggc gttgatccgc accaaaggcg aggctggtac cggcgacatc gtgcatgcgg
    18841 tcacccacct gcgcaccatt gtcaacgaca tgcgcagcct gacggtactc gatgaagccg
    18901 agctgtactc gcgcgccaaa gaactgcagg caccctacga actggtacgt ctggtggccg
    18961 aaaccggacg gctgccagtg ccgaattttt ctgccggcgg cgtagccaca ccagccgatg
    19021 cggcactgct gcgtcagctc ggagccgaag ccgtttttgt aggctcaggc atcttcatgc
    19081 agaatgcggc cgagttctgc ccgcctgccg aagcagagaa acgtgccagg gccatcgtac
    19141 gggccaccac acatttcgac gacgcggcca tcctgctcga aaccgctgcc gaactgcaag
    19201 gtgcgatgaa aggcctggcc gcagccagcc tgtcgccgga acagcaactc cagggccgcg
    19261 gctggtaatt acgatgcgca tcggcgtgct ggccctgcag ggcgattacg aagcgcatgg
    19321 cagacgcctg cacgacctgg gtgccagcgt ggtatgggtg cgcctgccgc agcatctgca
    19381 acgcattgcc ggcctggtca ttcccggcgg cgaatccacc acactgctgc atttcctgca
    19441 acgcgaacac ctgttcgatg cgctacgcgc gttctcacag gaaaagccct gcttcggcac
    19501 ctgcgccggt gccatcctgc tggcgagcga agtggagaat ccgtcccagc cctgtctggg
    19561 tgcaatcgat atcagcgtgc gtcgcaacgc ctggggccgt cagatcgaca gccacattca
    19621 cgccattccc accgtgcttg acgccgagcc catggaagcc gtctatatcc gcgctccacg
    19681 catcatccgc accggagcat cagtagagat tctcgcccgt gaccaggacg atccggtctg
    19741 ggtcaggcag ggacgtcaca tggccaccac cttccaccct gaactgtcgg acgacgcccg
    19801 ggtacacgcc gagttcctgc gcggcatgca gacggacaac ggctgacgca acccggtctg
    19861 acgccgtgac aaggcccagg ccgccagatt cagggttcag gggttgcggc ccgcgatacg
    19921 cgctcgcagg aaatccggca gatggttcag caacggcaac gccgccggat ccaccgcatc
    19981 aagcacattc ttgatcagca gtccggcctc ggtattgccc tccatcacca gccgtcggct
    20041 gaagaacagt gtgtccggat cttcctcgcg cagcgctagc cgggcaaaat cacgcgccgt
    20101 ggcggcaatg gtcacttccg gttgtgcctc gtgacggcgc ggagaaaaac cacgctggcc
    20161 aatcgccaga taaaacgaca accccagatc ggcaatacgg atgcacacgg tcttgccacg
    20221 gacgctgtcg agccggtcca gctcgatatg gcgtaccagc acgcgattga gcaacgctgc
    20281 cagcatcacc gtatgcggtg acggccattg ccagggcgac agcgaagccg caaacgggac
    20341 tcgggacagc aaacgcgaag aaacagacat cattcgactc ctgacgactc acatgtaact
    20401 gggctggcga ccggtaccag tcggatcacc ttgcgcaggg cgtccacgtc gagcgtagcc
    20461 tgcgcctcga atttgccggc atacgccagc tcttcctgct tgagctcgta acagttcgag
    20521 ccgctgatgt ccatcagcat ggccggcaac cctgcctgct gaaaggcccg gtgacgcttg
    20581 atgaaaaagc tgctgcagca catgccgatg taggccggca caccgcgtgc tttcatgtca
    20641 cccagcgtcc gttccagatg ttcgtaattc atgatggtgg tgacctgcat accacgctcg
    20701 cgcgccagcc ggtaagcctc gccgacttcg cacaggccgc attccgggca cccgtcgcgc
    20761 atacgccact tgcaccacgc cggttttgcg caatagggca ccagcatcac cgtcgcgtgg
    20821 ccgagaagct cgcccggcgc ctgtccccgg ggggtataca gcatcagcgc gttcgtttgc
    20881 gcgttgtcca gcccgatttc ccgttgcaac cggcgtttat ctgccagaat tgccagcagt
    20941 cgcgcgatgt cttcagcgcc gaacccgggc atgtcggcaa catggcgccg ggcataggca
    21001 agcgccaccg ccggcgctgc gtcaggcgtc gtcgcctgca gcgcatcctg cagccccggc
    21061 agccagccgg caggctcgat atggatatcg ccagccagtt cgactccgtc cagctggccg
    21121 gccgcattct cgcgagcacg aacgcgcaat gtaccaccag cggttttcca cagcgattcc
    21181 atgccacctg tctggatggt gcgccagtcg agccgccaca attcttccgt ttcgaccgac
    21241 tgccactgcg gcggcagacg ccgctcgccg gcatcggcag tcatgccgcc aaaattccgt
    21301 ccgagcaacg tcgccagccc ttgcaccaca accgaacgca cccgctggac atcgatgtcc
    21361 tcaccgagac attggcgcag ggtgatcagc cgctcgcgcg cagccgacag tccgtcgcgg
    21421 gacagttttt ccgtcggtgc gcgtaatacc tcaagcatgg tacggatatt ggcatcgagg
    21481 aggatcaccc cctgcaccag cagcgaactt ccattacgcc caatgaacaa tgaagcgagc
    21541 ttgcgcccgc caatttccac atcattcgga tatttgtaat gcgcatccgc acccagatgc
    21601 tgcaacccgg cggcgagtgc actggcaaac agcgccagca gctgggcttg acccaccgac
    21661 tcccagcccg gtggctggtg cagcagcaac gaccagcaca gctggtcagg atcgaggtac
    21721 agcgccccgc cgccgctaca gcgccgcacc atggcgatgc cggcctgctc gcaatgctca
    21781 atacgcagtt cacggtcaat cacctggtat cggccgatgg ctgcagtcgg cagactgcga
    21841 tggaagcgca actgatcgcc gagttcaccg cacgcatgac gcgcccaatc atgacgatcg
    21901 cgcgcaaaat tgatggctcc ggactgcagc ccgctatcac acagcgccac acatccggtc
    21961 actacgggtc tttcggttac gttcacggga gacctgcccg ctgtgtctgg tccatgcccg
    22021 ggcggtcgtg ccagtagccg ttgcagcttt ccatctcgcc cagacgatcc agccgggctg
    22081 ccgcagcaac cggggtgatg cccccctcga ccgcctcacg gatcacggca acgatctcgc
    22141 ccgtgttccg gctctgcggg ctgatgcgca gcacatccac ccccaggctg cgcatggcat
    22201 cgagctcgga gatcagattg catacacgcg aagactgtgt ctggatgcca ttgagcacga
    22261 gaaaggcctg gtcgtcgcgc gtgctcatgt ccagtccgtc cggataatcc atacagcgga
    22321 actggcagtc atccttgggc agattgtgtg cacgcgcggt aaagcagcgt gccgaatacg
    22381 ccagcggcat gcgtccccag acaaacacct ccgtctgcat cccttgcggc tgcgcggcac
    22441 agatcgtctg cagcatgtcc atcgaggcct cgaccggcgc tacccagcgc cgcgcaccca
    22501 gtgccgccag ctgtttcagc gtctggctgt tgtagacatt gaggtgggtt ccggcgacga
    22561 acccttccgt gccgctcatc aggcgcactg cacccatctc gttggcttcg accatgaatt
    22621 cgccattgcc ggcaatgttg cgtagcgtct tgaggtcgga ttcggattcg atcagcacct
    22681 gcgtcgacag tacgacttcc ttgcccgcat cgcgcaaccg ggcagcggtt tccagccagt
    22741 cgggcaggcg gaaactgtgg cggcgcgaac atacggtttc gcccagatag acaatatcca
    22801 gcggcgcttg cgccattgcc gcgtaaaact gctgcaactg ctcgcgcggc caataataca
    22861 gtacgggtcc cagtgccagt ttcattgtgt catttccagg gtctgtgata tgcgcccagg
    22921 gtgtgctgct gaccctcggc caccttgttc agttccgcca tccaggcgcc ctgaacgacg
    22981 aactgctgcg gtgcgcggta gcaggtgtcg atcgcattgc gccacacccg ggtaacctgc
    23041 gccacatacg ccgggctgcg ctgccggcct tcgatcttga tggcggatac gccaatgcgt
    23101 gccagttcgg gcaacagctc cagcgtattg aggctggtcg gctcttcgat ggcgtagtac
    23161 gtttcgtcgt ccaccttgaa acgccccttg cacaacgtcg ggtaaccggc gttctcgtca
    23221 tcggcgtagc gatcgataag cacgccgttg aggcgcgatt ccatgcccag cggggttttc
    23281 tcccagcgca ccgccctggc cggcgaacat accccggcgg tgttgggtga ttcgccagtc
    23341 acataggacg acagcgcgca gcggccttcc accatgacgc acaggctgcc aaagccgaac
    23401 acttcg
//
LOCUS       DAIANL010000072        14131 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2333752, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000072 DAIANL010000000
VERSION     DAIANL010000072.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 14131)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14131)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14131
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2333752"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2716 a         3886 c         4532 g         2997 t
ORIGIN      
        1 actgaacgga aagcaaataa ctgccattct cggcgttttt gacatttttg tcatgtcaat
       61 tttgtcaatt ggcgcggcca tgggttttga atggttgcca tgcgagtgca gccgtgcaac
      121 acaatccgaa gggggtgagc ggtatattgt aaaaaatcat ttatctttca tggtgatgag
      181 ggtgtggcgt taacggtctt gcaggttgtg ctggtatttt ttggcaggtg aagcgggaat
      241 tcgaaatctg gcacgctgtt tgcagtaagc cgtgatgcgt cgttgtgaca catggtgctg
      301 tatccgtccg ggctgatatt ctggacgaag caacacctca tcttgaggag gctgcgatgc
      361 gtgagatgtg gaatcatcct ttaggcaggc acatcatcgt gatcctgctg gtgaagctgg
      421 ttgccattta cgcgatatgg tggtttttct ttcggccgtt attggcatcc ggcacgccgg
      481 atgcggccag ggtgagcgcc gtgatggtcg gaacgcctgg cgtcccggcc gaggggcaca
      541 acgctcaatc ttctgtcgct accaaggagt caatgaaatg attagcaccg agctggtaga
      601 catatcgcga ttgcagttcg cgatggtcgc actgttccat tttctgttcg ttccgctcac
      661 gcttggcatg acttttttgc tggtgatcat ggaatccact tacgtggtga ccggcaagca
      721 ggtctacaag gacatgaccc agttctgggg caaactgttc ggcatcaatt tcgcgcttgg
      781 cgtgaccacc ggcattacta tggagttcca gttcggcacc aactgggcgt actactcgca
      841 ttatgtaggc gatatcttcg gtgctccgct ggcgatcgaa cggttgatgg cctttttcct
      901 cgaatcgaca ttcatcgggc tgttcttttt tggctgggag aaactgtcca aggtcaagca
      961 tctgatggtg acgatgctgg ttgcgctcgg ttccaatctg tcggcattgt ggattctggt
     1021 ggccaacggc tggatgaaca acccggttgg gtcggacttt tcgtacgtca ccatgcggat
     1081 ggaaatgacc ggtttctggg acgtgatctt taacccgcag gcacaggcca agttcgtgca
     1141 taccgtcagc gcgggttatg tgacggcttc ggtattcgtg ctggggattt cttcctggta
     1201 tctgctcaag gggcgtgaca tcgagtttgc caagcgttcg tttcgcattg ctgccgcgtt
     1261 cggtttcgcc tcggcgttgt cggtgatcgt gcttggagat gaatcgggtt ataccgtcgg
     1321 tgccaatcag gaaaccaaaa tggcggcaat cgaggccatg tggcataccg agcctgcgcc
     1381 agcatcattc aatctgatcg caggtgtcga tgaagcgcat cagaagaacg actgggccat
     1441 cgagatcccg tatgtcatgg ggctgatcgg cacgcgatcc accaccaaga ccatacccgg
     1501 cattgccgaa atcaaggcgc gtaatctggt gcgtatccag aacggcattc atgccgtgac
     1561 tgcactggcg gcgctgcgtg ccaatccggc ggatgccgtg gcgcgtgccg agttcgatcg
     1621 atacaaggcc gatctgggtt tcggcctgct gctgaagaaa tacaccagcg atgtcagcaa
     1681 ggctacgccg gacatgatac gcatggcgga tgatgacacg ataccgcgcg tattgccgct
     1741 gttctggaca ttccgtttca tggtggcgat tggcttcggt ctgctgctgc tgttcgtgct
     1801 cgcgctttac gcttcggtaa agaacgattt tgtgcagaaa cggtggctgt tgcgctgggc
     1861 cgtgtggttt atccccgtgc cctggatggc ggcggagatg ggctggttcg ttgccgaata
     1921 tggtcgtcag ccgtggacca tctatggcgt gctgccgacc tggctgtcaa cttccacgct
     1981 gacatcggcc agtgtgtatg catcgctgac cggtttcctg ctgctgtatg cggggctgtt
     2041 cgtggtggag atgtacctga tggtcaagtt cgcgcgccag gggccaagca gtctgtacac
     2101 cgggcgctat gccggcgaaa ccgcacaagg tgcagtggcg tgagcggtaa gacaattttc
     2161 gccgccggat ctgcggttgc aggtgtccgg tggcatgcat cgggagagaa tcatgttgga
     2221 ttatgaagtg ctcaagttga tctggtggct gttcgtcggc gtgttgctgc tcggtttcgc
     2281 cataatggac ggccatgaca tgggggtggg tacgttgctg cccttcgttg gcaagaacga
     2341 caatgagcgt cgggtggtca tcaataccgt ggggccgcac tgggacggca accaggtctg
     2401 gttcatcacc ggtggcggtg ccattttcgc ggcctggccg ctggtgtacg ccacggcgtt
     2461 ttccgggttt tactgggcga tgctggcggt gttgtggtcg ctgttctttc gaccggtcgg
     2521 cttcgactac cgctccaaga ttgccagccc gctctggcgc agcacctggg actggggcct
     2581 gttcatcggc ggagcagtgc ctccgttgat ctttggcgtg gcgtttggca atctgctgaa
     2641 gggcgtgccg ttccattttg acaatgacct gcgttcatat tacaccggaa gtttctgggc
     2701 gctgctcaat ccgttcgcgc tgctggccgg cctggtgagc acgacgatga tcactgcgca
     2761 tggcgccaat tttctgctga tccgtaccga gggcgatgtc taccgccggg cgcgcacggc
     2821 atcactgctg tttggtgcat tgtcgttgtt gacgtttgct gcagcgggcg tctgggtgat
     2881 gcacggcatc aatggctttg tgctgaccgg cggcgatttg cccggcggat tgcccaatcc
     2941 gatggacaag acggtagtga ccgcgccggg tgcgtggttg cataactact cggcctatcc
     3001 gctgacactg ctggtgccgg ctgcggcttt tgccggtgga ctggtatcgg tagtggcagc
     3061 gctggccaac cggccactca cggcgtttat cggttcgtcg gtcaacatgc tgggtatcat
     3121 cggcactgcg ggggtgtcga tgttcccatt catcatgccg tcttcgaccg acccgcgttc
     3181 gagcctgacg gtgtgggaca gcgtgtcttc gcagaccacg ctggggctga tgttctgggc
     3241 gacgctgatt ttccttccgc tggtgatttt ttatacgagc tgggcataca aggtgatgtc
     3301 tggcaaagtg acgcttgatt atgtcaaggg tcatgaccac tcggcctact aacagggagc
     3361 gcgatcatgt ggtactttac ctggtttctt ggtgtggcaa tggctgtgtt gctggccatt
     3421 gtcaacgcgg tttatggcga aaatcacgag atgaacgatg attcgggccc ggcaggtcac
     3481 tgacgtgcat ttgcggctgg cacgcgcgct gtcgctgctg ctggccggca tgctggccgc
     3541 catggtcacg ttatatccac cggcactggc acgcatgggc cacggtgaga tctcgctcgt
     3601 ggtctggggg atatgtgccg gattcgtgca tggcataggc tttgtgcctg attcccgggt
     3661 gtggcgcacg ctgttctcgc cgtggctggc ctggttgttg atgggctttg gcgtgatgcg
     3721 catggccggg atgtgaacac ggtgtttacc actacggagg gcagaatgag tttgtttcgt
     3781 cagctattcg atgagacaag ttcgacctat acttatgtgc tgggtgatgc cgtgagcggt
     3841 gaggcggtat tgatcgatcc ggtgctgggt caggaaaacc tgtacctttc gctgctcgac
     3901 gaacagcacc tgaggctggt gctggttctg gaaacgcatg tgcatgcgga tcacgtgacc
     3961 ggagccgcgc ggctgcgcga ggcaaccggc gcacgcattg gcatcagcgc gtcagccggt
     4021 gcgccgtgcg cagactggca gttgcttggt catgagacac tggacttcgg cggcgaacat
     4081 atccaggtgc tcgctacgcc gggacataca ccggattgcc tgtcctacct gtggcgcgac
     4141 cgcctgtttt gcggcgatgc gttgctgatt ggcgggtgcg ggcgcaccga tctgcaaggg
     4201 ggtgatgcgg gcgcgctgta tgacagcatc ctgcgagaga tctggccgct gccggacgaa
     4261 acgctggtgt atccggcgca cgattaccag caccggcatg tttcgtgcgt gagccaggag
     4321 cgcgagacca atgcccggct ggcgggtcgc tcgcgcgacg aattcattgc tgcgatgaac
     4381 cagctggagc tggcgcagcc aaaaatggcg gggcaggcga ttcctgccaa tagacagtgt
     4441 ggagaaccaa ttgttcgtac agcctgagaa agtcattgcg acgcattatg aacgcatcgg
     4501 tggcgaagaa acgttgcgcc ggctggtgca ccgcttctat gccatcatgg atgaactgcc
     4561 ggaagcctgg ggcatccgca agctgcatgc tgaagacctg cagggatcgg aggacaagct
     4621 gttcaagttc ctctcgggct ggatgggggg ccctcagctg tatattgagg cctatggtca
     4681 tcccatgctg cgttttcgtc atctgccgtt ttccattggc agggatgagc gtgaccagtg
     4741 gctgatgtgc atggatatgg cgctcaatga aacagtggcg gacgaagctt tgcggcgtga
     4801 actgctggtc gctttcgcca aggtgggcga ccatatgcgc aaccgtgccg agcctgccga
     4861 tgcaccagtc cactctacag aaggaatgca ttcatgatga ctccccaaca gctggttgcc
     4921 caggccaaga gttgcatccg cgaagttgat gtgcccgcag cagcgcagat gatcgccagc
     4981 ggtgcccagg tgatcgacgt gcgcgaagaa cacgaatttc ttgccggcaa tttgccaggc
     5041 gcggtgcata tctcgcgtgg tgtgcttgaa ttcaagatca gtgaacagcc cgcgtttgcc
     5101 gacaagtcgg caccattgct ggtgtattgc cgcaccggcg gtcgttcggc tctggccgtg
     5161 caatcgttgc aattgctcgg ttataccggt gcggtgtccc tggccggtgg ttatgtggcc
     5221 tgggtggcgc atcatgagcc ggttgttgaa gacaaaacca gtttcggcgg ttagttcact
     5281 cgaaaaattg attcaacggg ttgtgcgtca ttcatattat aatatgctta tatagcgaaa
     5341 ttactttgcc ggacggctga gcattccggc ggtctgtttt attaatcagg aagaaagtat
     5401 ctcacatggc acacatcgtt gtattgggcg ccgggaccgg cggaacgcct gcagcctatg
     5461 aattgcgcga ggcgctggac aagtcgcacc aggtgacctt gatcaacgcc tccgaaactt
     5521 tccagttcgt gccttccaat ccatgggtgg cgctgggctg gcgcaagcgt gaggaaacca
     5581 cgatccagat ccggccctat ctggagcggc gtggcatcaa tttcatcgcc cagcgtgctg
     5641 agaagatcga cgctgccgcc aaccgcatcc agctcgcaga cggacagacg gttgactacg
     5701 attatctggt gatcaccact ggtccgcgtc tggcatttga tctgatcgaa ggcgcgggtc
     5761 ccgatgcggg caatacccat tcggtgtgca ccgtcgatca tgcggaaaaa gcgttcaccg
     5821 attacgaaca gtttctcgaa caccccggcc aggtggtcgt gggtgcattc cagggggcat
     5881 cgtgtttcgg cccggcctac gagtatgcgc tggcgctcga gaccgatctg cgtcgtcgca
     5941 agattcgtcc gcaggtaccc attaccttcg tgacttccga accctacatc ggccatcttg
     6001 gtcttggcgg cgtgggcgat tccaaggggt tgctggagag cgaactgcgc agccgccaca
     6061 tcaagtggat caccaacgcc aagacagtca aggtcgaagc gggcaagatg tacgtcgagg
     6121 aatacaatga ccagggcgaa aaaatccgcg atcacgtgct cgagttcaag tattcgatga
     6181 tgttgccgcc gttccgtggc gtgccggcag tggctgcggt ggagaacctg tgcaatcccg
     6241 gcggattcgt catcgtggac aaataccagc gcagccctgc gtacaagaat atcttttccg
     6301 ctggcgtgtg tatcgccatt ccaccggtgg aacaaacccc ggtaccgacc ggaacgccca
     6361 agaccggtta catgatcgag tccatggtga cagccatcgt gcacaacatc agggacgaac
     6421 tggacggcaa gccggcggac actaccggca cctggaacac catctgcctg gccgacatgg
     6481 gcgataccgg tgctgcgttc gtggctctgc cgcaaattcc gccgcgcaac gtgacctggt
     6541 cgaaaaaggg caagtgggtg cacctggcca aggtcgcgtt cgaaaagtat ttcatgtaca
     6601 agatgaaaac cggtcattcc gaacccattt acgagaagta tacgctcaag gcattgggca
     6661 tcgtgcgtct gagcgagcac atgaccacga agaaatagcc tgcaggccgg atggccatgg
     6721 ccggaataaa cagggccgca tttgcggcct tgttgccgtc cgggtttatt atcggcgtca
     6781 gctctgttat cctgcgcggt tccggcgcat cgcttgttgc ggacatgatc aagactgacg
     6841 tactcatcat cggcagcggt ctcgctgcac tgaccacggc actccacctc gctgaccgca
     6901 tgcaggtgac ggtggtaacc aagaaatcat tgcaggattc cgccagtgcc tgggcacagg
     6961 gtggcatcgc cgccgtgctg gccgatgacg atacgatgga agcccacgtt gccgatacgc
     7021 tggtggctgg tgccggattg tgccatgagg aaaccgtgcg tttcgtggtg gaaaacgcgc
     7081 ggcccgcgat cgagtggctg gtaggtctcg gtgtgccgtt cactgccgat gatgccagca
     7141 gcaccggatt ccatctgacg cgagagggtg ggcacagcgc acggcgcatc atccatgcag
     7201 ccgatgcgac cgggcgcgcc gtgcaacgca cgctgtgcga gcagatgcgc gaacatccca
     7261 acatccgcct gctcgaacgc catatcgcgg tcgatctgat caccggcagc cgcatcgggc
     7321 tggagccgga ccgctgctac ggctgttatg tgctcgacct ggagcaggat cgggtggaga
     7381 cctgggttgc cagtcacacg gtgctggcta cgggcggcgc gggcaaggtg tatctgtata
     7441 ccaccaatcc cgatacctcg accggcgacg gcgtggccat ggcatggcgg gccggatgca
     7501 gcgtcgccaa cctggaattc gtgcagtttc atccgacgtg tctctaccac ccgcatgcca
     7561 agtcgtttct gatttcggag gcggtgcgtg gcgagggggg gctgctcaag ctgcctgacg
     7621 gcacacgttt catgccgcgt tacgatgcgc gtgcggaact ggcgccgcgc gacatcgtgg
     7681 cgcgagcgat cgacagcgaa atgaaaaaac gcggactgga ctgcgtgtat ctcgacattt
     7741 cgcaccagcc ggccgagttt atccgcgagc attttcccaa tatccacgag cgctgtctca
     7801 gtctgggcat cgacatgaca cgcgagccga tcccggtggt gccggcagcg cactacacgt
     7861 gtggcggcgt ggtgaccgat ctggacggtc gcaccgaaat cgaccgtctg tacgccattg
     7921 gtgaagtatc gtgcagtggg ctgcatggtg ccaaccggct ggcgagcaat tcgttgctcg
     7981 aatgcctggt gttcgggcgg gctgcggcac aggccattcg cgccagcggc acgatgccgc
     8041 tacccgaggt gccggaatgg gacgacagcc gggtgtcgga tgccgaggaa gaaatcctgc
     8101 tgtcgcacaa ttgggacgaa ctgcgtcgcg ccatgtggga ttatgtcggc attgtgcgta
     8161 ccaacaagcg cctggagcat gccgcgcgcc gtatcgccct gctgactgaa gaaatcggcg
     8221 aatattatcg tgatttccgg gtcagcaacg acctgatcga attacgcaac ctggtgatca
     8281 ctgccagcct gatcgtgcgc tcggcgcagt tgcgcaccga aagtcgcggg ttgcatacca
     8341 gtctggatta tccggcgctg atggcctcgc cgatggatac ggtgctgcgc cagcgcatgc
     8401 gcaagccacg ttacagtgag acggattcga gatcgctgga cgccgactgg gagtagtcga
     8461 acagatcctc ccaggccgtg aaatagctga tgaacccgac cgggatgacc accaggaagc
     8521 caagcagcat gggcaggctg cccaccacca gcaacaccac catcaggatg ccatagagca
     8581 ggaatggcgc gaaattggcc gtgcaggcac tcaggctgag tttcatcgct tccagggcct
     8641 ggaaatcggc cagcaccacc agtgctggtg cgaaccagta ggccatgagc atcagcgtga
     8701 acagggtcag gctgaacagc aataccagcg ctgcggcgag gctcgggtgc agggcatcga
     8761 gcggaggggg agtggttgtt tgtgtgctgt agagcagcgc gtgaatcaga ggatcattgc
     8821 tgaacaacgc cggcaacgta aacgcaatga gcatgccggt catgtagata ccaccgatag
     8881 tgaccagtcg cgagccatgg ctgttgaacg ccgagaacag gtgtttgaag gcaagtgtct
     8941 ccccgcgctg ttgtgccttg caggccagca tcagcccgcc attgaatacc ggggcgatca
     9001 actggaacac cagcgtgccg gcgaatggca ccagactcat tgcagcgccg atcagcacga
     9061 tggttacgct gtagccaatc cacatcagcg gatggtggcg gtacatgtcg aatgccgtgc
     9121 gtatccagcg atagccgtgg gacaggggta cggtacgaaa cggaatgcgg ttcatgtcag
     9181 tggatccaca tattgcgcag cagcggttgc gagcggatgc ggtcgagcag gatggtgcgg
     9241 aaatggttcg gatccttggc gtgcgtcatt tcaccttcgc gtggaaagtg cagatcgagc
     9301 agacgcgata cccagaagcg cagtgcagcc gcgcgcagca ctaccggcca ggcaccgcgt
     9361 tcgatggcgg tcagcggacg caccgcgtga taggcattga gcagggcaag gcaccgttcg
     9421 ctgtgcaacg atccatcgtc ttccacgcac cagtcgttgg tggtgatggc gaggtcatac
     9481 agccagttgt cgctgcaggc gaagtagaaa tcgatgaccc cggtcaggcg gccgctgtcg
     9541 aacagggcgt tgtcacggaa caggtcggcg tggatgatgc cgcgcggcag gtcttgcagc
     9601 cggtagagcg actggtggcg gatttcctgt tgcagcagtt cggcgccggc tgcgtccagc
     9661 aggggcagca gtcgcggcgc ggctgccttc caccaggccg gtccgcgagg gttgtcgcgc
     9721 tgcaggggga agtttgcacc agcgagatgg atgcgcgcaa gcgtggcgcc gacggactcg
     9781 cagtggtgca gtgcgggttc ctggatcgag gtaccctgga ggcggctcac gatgctggct
     9841 ggcttgccgt gcagatgctc gagcatatcg ccgttgcggt tggcgaccgg atgcgggcag
     9901 ggcacaccgt gatcggccag gtgcgacatc agttgaagga aaaatggcaa ttcggtggct
     9961 gacagccgct cgaacagcgt cagtacgtaa cggctgcggc tggtatcgag aaagtagttg
    10021 gtgttctcga taccggaagc gatgccttgc aggccgttca ggttgcccag atcgaaacgc
    10081 cggagcagtt cgctggcgtc agcttcggtg acacgggtga atacggacat ggcttataca
    10141 caggttacca ctgatgcagg acccacatcg gcggtgacac ggcgccatgc accaggtcct
    10201 tgcgcacgaa ctggccatcg ccgcgtgggt cgacgaggta gtacggccgg cctctggccg
    10261 gcgtgacgcg gatcatgtag agcatgccgt ggatacggta ttcctcgtac ctgtcgctgc
    10321 catgctgctt gatcacgatc tgcggttcgt cggagctgtc gtccggcaga ggcgctggcg
    10381 gaacggctga gccggccgcg gtggcgggcg ggctggcggc cggctggtcg gctgcggcca
    10441 gcagcgggca gagcatcagc aatgcaagca aaagggaacg cacggaaaac tcctgatatt
    10501 tcaccatgtc tgcattctag cagagccggg gcggttataa atagagctgg atttcccgat
    10561 cgtgcgcgga aggttcgaag ccctgctgtt catagtggtc gaaaatggcg cacactaccg
    10621 cttccggatc gtcgatcacc ttgatcagat ccatgtcttc cgggctgatg agctgttcgg
    10681 ccaccagtga atcgcggaac cagtccacca gtccgcgcca gaaagagctt tgtaccagga
    10741 tgatgggaat cttccgcgtc ttgccggtct gcaccagcgt cagcgcttcc atgagttcgt
    10801 ccatggtgcc gaatccacct ggcatgacca cgtacgccga ggcgaacttg acgaacatca
    10861 ccttgcgcac gaagaaatgc gcgaaggttt gcgatatgtc ctggaacgga ttggtatgct
    10921 gttcgtgtgg caactggatg ttgaggccga cgctcggtga cgggccaaag aatgccccct
    10981 tgttggcagc ttccatgaca ccgggcccgc cgccggagat caccgagaat ccggcgtcgg
    11041 acagcttgcg cgagatgcgt tcggtcagtt cgtaataggc atgaccgggg gggatgcggg
    11101 cgctgccgaa gatgctgacc gctggtcgga ttccttccag gcgctcggtt gccgatacga
    11161 attctgacat aatctcgaag atgcgccatg actcgcgggc gttgtagctc ggatgattgg
    11221 cctgctcggg attgatttgc cggggaagtt tttcgttttg catgattgat ggggacgtgt
    11281 tagtgaccaa aacattgctg ctggtggatg gatcgtcgta cctgtaccgc gcatttcatg
    11341 cgctgcccga catgcgcagc ccggatggcg aaccggtcag cgcgattttc ggtgtgctga
    11401 atatgctgcg tcggctgcag tcggattttg cggccgatta taacgcgtgc gtgtttgatg
    11461 caaaaggaaa aacatttcgc gacgactggt atccgcaata caaggcgcat cgcccaccca
    11521 tgcccgacga tctggccagt cagatcgaac cgttgcagca ggccattcgc gcactgggct
    11581 ggccactgct catggtcgat ggcgtcgagg ccgacgatgt gatcggcacg ctggcgcacc
    11641 atgcctcgca gcacgggatt gataccatca tttccaccgg cgacaaggac atggcgcaac
    11701 tggtgagccc gcaggtgcgg ctcatcaata ccatgaccga ggagttgctc gacgaggagg
    11761 gggtgcgggc caagttcggc gtgccgccgt cgggcatcat cgattacctc acgctggttg
    11821 gcgacgctgt ggacaacgtc ccgggcgtgc acaaggtcgg gccgaagact gccgtcaaat
    11881 ggctgcagca ctacgggtcg ctcgatgccc tgatggcgca ggccgccagt gtcggcggag
    11941 cggtgggcca gaacctgcgc gatgcgctcg actggctgcc cacggcacgc agactactca
    12001 ccatacgtac cgatcttgaa ctcgaactac cggtcaccgc actggtcatg cgtcagcgcg
    12061 atgtggccct gttacggcag attttccagc gctaccgttt ccgtacctgg ttgcgcgagc
    12121 ttgatgagac ggccgacccg gtcgatccgg ccgatgccgg caattcgctg ccggctgcaa
    12181 cgcaggcgca gcgggctgcg caccattacg aaaccatcac cagcctggcg cagctcgatg
    12241 tctggctgac gaagttgcgc gccgcagcgc tggtcagcgt cgacaccgag accaccgggc
    12301 tcgatccgtt gcgagcagcg ctggtgggca tgtcgttttg cgtcacgccg catcaggcag
    12361 cctatctgcc gcttgcgcac cgcgggccgg atgcggccga tcagccggac atgcagcagg
    12421 cgcttgaacg gctgcgtccc tggctggaag acccgacgag tgccaaggtg gggcagaaca
    12481 tcaaatacga catgcacgta ctcgccaacc atggcatccg cctggatggc gtggcgcatg
    12541 acacactggt cgaggcctac ctgctggaat cgcatctgcc gcgcaatatg gacagcctgg
    12601 cgttgcgcca cctgggcgtg tcggtcatca gctatgatgc ggtgaccggc aagggggctg
    12661 cgcgcatcgg tttcgaccag gtcgaaatcg cacgcgctac cgaatacgcg gccgaagacg
    12721 ccgacatcac cctgcagttg caccagtgtc tgttgccgcg gctgacggac gacgcggggc
    12781 ttgaccgggt ctaccgcggc atagagatgc ccctgttgcc cattctctgg cagatggagc
    12841 gcacgggcgt gctgctcgat tgcgcgttgc tggaggagca aagccgcgag ctggggcagt
    12901 cgatgctgca gctcgaacgc cgggcagtcg aactggccgg gcagccgttc aatctcaatt
    12961 ccacgcgcca gatccaggcc atcctgttcg agcagcaggg gctgcctgta ctgaaaaaga
    13021 caccgggtgg ggcaccatcg accgatgaag acgtgctggc gcggctcgca gccgattatc
    13081 cgctgccgcg ctgtctgctg gattaccgcg cgatggcaaa actcaaatcc acctataccg
    13141 acaagctgcc gcgcatggtt aatccggata cgggccgtgt acacaccagt tattcgcaga
    13201 ccgtggccgt gaccggtcgc ctgtccagcg tcgaccccaa tttgcagaat atcccggtca
    13261 agaccgcgga ggggcgccgc atccgtgaag ccttcatcgc ggcgccgggg catgtgctgg
    13321 tgtcggttga ctattcgcag atcgagttgc gcatcatggc gcacctgtcg caggacgata
    13381 acctggtgcg ggcttttgcc gatgacgctg acattcaccg cgccacggca gcggaaattt
    13441 ttgccattgc tccggaagac gtgggcaacg agcaacgccg ggttgcaaag accatcaatt
    13501 tcgggttaat ttacggtatg tcggccttcg ggctggccag tcagctgggt atcgagcgcg
    13561 cggccgctca agcctggatt gatcgctact tcgcccgtta ccccggggtg gcagcgtaca
    13621 tgcaacgtac ccgcgatgcc gcgcgagcgc aagggtatgt gcagaccgtg ttcggacgcc
    13681 ggctctggct gccggaaatc aggagtccga acaaacagcg tcgtgatgcg gcggaacgcg
    13741 ccgcgatcaa tgcgcccatg cagggtactg cagccgatct catcaaactg gcgatggtgg
    13801 cggtatccga ttggctcagg cgggaagaca tgcatagccg gctcatcatg caggtgcacg
    13861 atgaactggt gctggaagtt ccgaacctgg aacagcctgc agtcgaacgc tatctgcccg
    13921 aactgatgtg ctcggtgacg gaactgcgcg ttccgttgcg cgtgtccatg ggctctggtc
    13981 cgaattggga agcggcgcat tgacactgga atgcctgttt catccccaaa ttttgacctc
    14041 ggccgcattc ggtattaaca ttcacggttc tattggggaa atgaagtggc aacggcgatg
    14101 gaattgacag gcgcggaaat cacggtgcgt t
//
LOCUS       DAIANL010000073         6670 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_697394, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000073 DAIANL010000000
VERSION     DAIANL010000073.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697394"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1080 a         1813 c         2381 g         1396 t
ORIGIN      
        1 accatgggcg gcgccctgat tctggtgtcg attgcgctgt cgacattgtt gtggggcgat
       61 ctggataacc actacgtgtg gattgcgctg cttgtcacac tcggttatgg cgcgatcggc
      121 tgggtggacg attatcgcaa ggtggtgcat cgcaatccta aaggactgtc agcaagagaa
      181 aaatatttat ggcaatcgct gatcggcgtg ggcgcggcac tgtacctcgc ctattcagcg
      241 cagttgccgg cgcagaccga gctgatcgtg ccgtttttca agcacgtggc catcccgctg
      301 ggcacggcag gattcgtgct gctgacctat ttcgtcatcg tcggcaccag caacgcggta
      361 aacctgacag acgggctgga tggtctggcc atcctgccga cagtcatggt tgcgtcggca
      421 ttgtccatat ttgcctatgt ggccgggcat gcggtgtttt cgcattatct gggcgagccg
      481 catattccgg gagccggcga gctggctgtg ttctgcgctg caatatccgg tgccgggctg
      541 gcgttcctct ggttcaacgc ctatcccgca gaagtgttca tgggggatgt gggcgcactg
      601 gcgctcgggg ctgcacttgg tacggtggcg gtgatcgtgc ggcaggaaat cgtgctgctg
      661 gtgatggggg gcgtgttcgt tatggagacc ctgtcggtga tgattcaggt cgcttcgttc
      721 aagctgaccg ggaaacgggt atttcgcatg gcaccgctgc accatcactt cgaactcaaa
      781 ggctggaaag aaacacaggt ggtggtgcgc ttctggatca tcaccatgat gctggtgctg
      841 atcggattgt cgacattgaa aattcgctga aatggaaaac acagcatgaa ctgggtgggt
      901 gaacgcgtac tgatacttgg gctgggcgcc agcgggcagg ctgcggccgg ctggctggcg
      961 cgccagggtg cccaggtgcg cgtggccgat cagggtgagg cgccgtctgg tctggagggt
     1021 ttgcgcgcca actggccgga ggtgccggtg cggctcgggc catgggccga ggaggatttt
     1081 tcctgggcgc aacggattgt catcagcccg ggcgtgccgc aatccgatcc tgtggtgcag
     1141 gccgccgcag agcgcggcgt tccggtgtgg agcgatgtcg aagtgtttgc acgtgcgctg
     1201 ccggcgcagg cgcgggttat cgccattacc ggctcgaacg gcaaaagcac cgtgaccagt
     1261 atggtcgcag caatgtgcaa ggcggccggc tggaaaacgc gattggccgg aaacatcggt
     1321 atcccggtgc tgcaggcgct ggaagaggcg ccggataccc agatcttcgt gctcgagttg
     1381 tcgagtttcc agctggagaa catttatagc ctgcacagcg atgccgccgt ggtgctgaac
     1441 ctgagtgaag accatcttga tcggtatgca gacatggccg catatgtcgc agccaaggca
     1501 cgtatctatg cagatactgg catgcaggtg gtcaaccgtg acgatgcagc cgtggtcagg
     1561 ctcgcgcgag ctgatcggcc gctggtgctg ttcggtctgg acagaccaca gcggcatgcc
     1621 gatttcggcc tgatcgacgg acagctgtgc cgcggcgaga cgtcgttgct ggcggtgcgt
     1681 gaactccgcg tgaccgggac gcacaatgtt gccaatgcgc tcgcggcatg ggcactgtgc
     1741 catgcgctgg gtatggcgga cgaaccgctg gcgcgagcgc tgcgcgagtt tcgaggcctg
     1801 ccgcatcgcg tggagtgggt gagcagcatc gccggagcag attattacga cgattcgaaa
     1861 gggaccaatg tcggtgcgac cgaagccgcg gttgtcggca tggaccgccc ggtggttctg
     1921 attgcgggtg gtgagggcaa ggggcaggat tttgcgccgc ttgcgcgcgt ggccgtcggg
     1981 cgggtgcgtg cgctggtgca gattggtcgg gacgggcaac gcatcgccga tgtttttgcc
     2041 gcggtgggca tcgaaacgca tcgtgcagaa agcatggacg cggcggtcgg tctggcacac
     2101 gcgctggcgc agccggggga tgcggtgttg ctgtcacccg cgtgcgccag tttcgacatg
     2161 ttcgacaatt acgcgcaccg cgcacgcgtg ttcgtgaccg cggtgcagcg tctcgcggga
     2221 ggacagccat gagtgcggtg caaaccagcc gcaccagcag tgcgcgcatt gatcccgtgc
     2281 tgctgtggct ggtgatcggc atgctgtcga tcgggctggt gatggtgtat tccgcgtcga
     2341 ttgacatggc ggaagcatca cgcagtaccg ggcaccgtgg ctattatttc ctgctgcgtc
     2401 aggcagggtt catcgccgtg ggcggcgtga caggcttcct cgcattccgg gtgccaacac
     2461 tgcactggca gcgggttgct ccgtggctgt tcgtcggtgg gctggtgctg ctggcagtgg
     2521 tgctggtgcc cggcatcggc cgcaatgtca atggcagcca gcgttggctt ccactcggtt
     2581 tcatcaacct gcagccttcc gaactgatga agctggcggc tgtgctctac gctgccgact
     2641 ataccgtgcg caaggcggct ttcatgcacc atttcaagca ggggttcctg cccatgctcg
     2701 tcgccatcct gttcaccggc gcactgctgc tgcgcgaacc ggattttggt gctttcgtgg
     2761 tcatcatcgc cattgccatg accacgttgt tcctcggtgg cctcaacctg agaatttttg
     2821 tcggtctgct ggtgctgtcg gtgattggtt tcatcgggct gatctggagc tcaccctacc
     2881 ggctgcaacg catcgtcggt ttcatggatc catgggccga tccgttcggt aaaggctacc
     2941 agctgtcgca cgcactgatt gctttcggtc gagggcaatg gctcggtctg ggtctggggc
     3001 gcagtgtgga aaagctgttt tatctgcctg aggcgcatac cgacttcctg ctggcggtca
     3061 tcgccgagga attggggttt gtcggtgtgg tggcggtcat cggcctattc atctggctgg
     3121 tgatacgggc gtttcgtatc ggacgcgaag cggcgcaact ggagcggcat ttctgtgcgt
     3181 tgacggcaat gggcatcggc gtatggatcg gtgtgcaggc cggcatcaac atcggcgtca
     3241 acgtcggcct gctgccgacc aagggactga ccttgccgct gctttcgttc ggcggcagcg
     3301 gtatcgttgc caattgcatt gccatggcga tcttgctgcg gatcgattac gaaacgcgcg
     3361 aactgcggcg cggcaggaag gggacgtgac atggcgcgca ccctgatgat catggcgggc
     3421 ggcacgggcg ggcacatcta ccccggactg gctgtggcgg aagaattgag ccggcgcggc
     3481 tggagcatcg tgtggcttgg cacacgcaaa gggatggagg gacgtatcgt accgcagcac
     3541 ggctatcgca tggcctggct gaatatcagt ggattgcgcg gcaacggctg gctgcgcaag
     3601 ctggttatgc ccggcatgct ggcctggtcg tgcgttcagg cgctgtgggc actgctgcac
     3661 tatcaacccg acgcggtgct gggcatgggg gggtatccga gcgtgccagg cgggctgatg
     3721 gccgcgctgt tgcgcaaacc gctgctggtc catgagcaga atgccattgc cgggctgagc
     3781 aatcgactgc tggcatgtct ggcgacccgg gtcatgtccg ggttcccgga tgcgttccgg
     3841 gctgcgaaaa ggatttggcc gtgccgcaag gttgacagcg aatggaccgg gaacccggta
     3901 cgtgcggaca tcgttgccgc aggcgcaaag cgcaggcacc acgagggccg gttgcggttg
     3961 ctggtggtcg gcggcagcct cggtgcagcg gcgctcaata ccgtggtgcc ggcggcgctg
     4021 gcgctgctga cggaagagga acggccattg gtcgtgcacc agtcgggcgc acgtcatctg
     4081 gaagtcctgc gggcgaatta tgtcgcagtc ggcgtcgatg ccgaggtgcg cgaattcatt
     4141 gtcgacatgg cagaaatgta cgcctggtgc gatgtggcta ttacccgtgc cggcgcactg
     4201 acgctggctg aactggcagc ggcgggcgtg ccggcgattc tggtgccgta tccgcatgcg
     4261 gtggatgatc accagacggt caatgccgga tttctcgtgg cgcgacaggc cgcgcggctg
     4321 atcagtcagg acggattgac cgccgagcgg ctggccgcgg aactgcggtt gctgaaccgc
     4381 gacctgcttg cagccatgtc gcgccaggcg ctggcagcgg cgcgaccgga ggcggtgatg
     4441 cgtgttgcgc aatgttgtga ggatgcggtg tcatgaaaca caaggtcagg caactgcatt
     4501 tcgtcggaat tggcggctcg ggcatgagcg gcatcgccga ggtgttgctc aacgaaggct
     4561 atgtcatcac cgggtcggac gtgtccgaca gcgccaatac gcgtcgcctg tgcgatctgg
     4621 gtgcgcacgt gtttttcggg catgccgccg agcatgtggg caaagccgat gcgatcgtgg
     4681 tctcgactgc ggtcagggaa gacaatccgg aggtggtggc ggcgcgcgcg cgttccatcc
     4741 cggtggtgcc gcgcgccatc atgcttgccg aactgatgcg tctgcgacag ggcatcgcca
     4801 tcgccggtac gcatggcaag accacgacca cttccctgat cgcaagtatc ctggctcagg
     4861 cagggttgga tccaacgttc gtgataggtg ggcggttgct ggcggcgggt gccaatgcgc
     4921 ggctgggtca gggcgactac ctggtggcgg aggcggacga atccgatgcc tcgtttttgc
     4981 acctgtcacc gatcctggcg gtagtgacca atatcgatgc cgaccacatg gaaacctacg
     5041 gtcacgattt ttcccgtttg caacaggctt tcgtcgactt cgtggaacgc attccgtttt
     5101 atggtcgcgc ggtattgtgt gccgatgacg ccaatgtgcg cgccatcatg tcacgcatca
     5161 gcaagccggt gacgacctat gccattgaat ccgaggcaga cctgcgtgca gtggacgtac
     5221 gtgccgaaga cgggcgcatg tgctttggtg tcgtcaatgg cggcggcaag cggttgatgg
     5281 agattacgct caacgtgccc ggcgtgcata acgtgctcaa cgcgctggca gcgattgcta
     5341 ttgcgttgga gatcggcgtg ccttatgcgg caatcgagcg cgccctggcg gaattctccg
     5401 gtgtgggccg acgcttccag cgttacggtg atgttccttt ggctgatggc acccgcttta
     5461 cgctggtcga tgactatggc caccatccgg cggaaatggc cgcaaccatt gccgccgccc
     5521 gcgcagcgtt tccagggcgc cgcttgttgc tggcctttca gccgcaccgc tatagccgca
     5581 cgcgcgacct gtttgaagat ttcgttgcgg tgctgggtag cgtcgatgcg ctgttgctta
     5641 ctgaagtgta tgcggccggc gaggcgccca ttgtcgcggc cgatgcgcgt gcgctggcac
     5701 gggcgctgcg cgtggccgga cggcttgaac cggtgtttgt cgcccgggtg gaagatctgg
     5761 cgcaggcggt gctggacaac gcccgtggtg gcgatgtggt actgaccatg ggggcggggt
     5821 cgatcggagc cgtgccggga cagatcgtgg aaaaaatgga gcagcgcggc catggcaacg
     5881 cagcgtcctg agatcgacat gactggcttg cggggccgca tgctgctgca tgcgccgatg
     5941 catcagcatg tctcctggaa ggcgggtggg gcagccgatg tgttgtatgt tccggccgat
     6001 ctggacgatt gggcgatgtt tcttcgacgc ctgccccggc atgagccggt gcattgtgtt
     6061 gggctgggca gcaacctgct ggtgcgcgat ggcggcgtgc ggggtgtggt catcgtgttg
     6121 catggcgccc tgcgcgaatt gcacgaggag gcggcctcgg tcggccaggg catgatgtac
     6181 gccgaagccg gcgtggcatt gccgcggctg gcccggtttt ccgccagttg cgggttggtg
     6241 ggtggcgaat tcatggtcgg gattcccggc accgtgggcg gcgcgctggc aatgaatgcg
     6301 ggctgttatg gcggcgagac ctggaatgtg gtggcgcgcg tgctcacaat gcggcgtgat
     6361 ggtagccgcc aggtgcgcga gcgtgaagaa ttttcggttg gatatcgcga agtgcgcgga
     6421 atggagcagg acgaatggtt cgccgcgggc tggttcgtgc tggcgcgcgg caatgggcag
     6481 gcggcgcgcg agttgatggc cgacctgctg cggcggcgcg ttgccagcca gccgctgggt
     6541 cagccgaatg ccgggtcggt atttcgcaat cctcccggag atcatgcggc acggttgatt
     6601 gagcagtgcg gactgaaggg ctggcagatc ggtgcggcgc aggtgtcgcg caagcatgcc
     6661 aatttcatcg
//
LOCUS       DAIANL010000074        10199 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_499489, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000074 DAIANL010000000
VERSION     DAIANL010000074.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 10199)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10199)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10199
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499489"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2047 a         3474 c         2902 g         1776 t
ORIGIN      
        1 gcttgccctt tttttcacca tagatgatcg ccggcaccat caggatgaaa gccaccacca
       61 ccacaggcag gtagacttcc cagtggtggt cgatgtccag atggccggtg cgctccagca
      121 ggaacgggat caccacgaac atcgacatct gcgccgaatg cagcgaaaaa atgccgaaat
      181 tgagccgcag cagctgggta ttgcgcagaa cgccgcccag cttggccgga ctggcttcgg
      241 catcgctgtg aaaatgactt gcacgtggat cgggaaccac ccacttgacc accgccatcg
      301 ccgcgatgga cagcacgccg gtcagcgcga agatgcctgg cataccgatg aattggtaca
      361 tggccggccc catcaccagg gacgcggcga aagtgagtcc gatggtgccg ccgatcatgc
      421 ccatggcacg cgtgcggtgt tcctcgcgcg tcgaatcggc cagcagcgcc gtcacggcag
      481 cggaaatcgc accggcaccc tggactaccc ggccgatgat gatggtttcg atattggtcg
      541 cgccagcggc aatgaaactg cccaacgcga acagtgcgag gccgagatag atcatgcgct
      601 tgcggccgaa gtgatcggat gccatgccga acggcagttg caacagcgct tgcgtcaggc
      661 cgtaagcacc gagcgcaagg cccaccaggg tacgatcctg cccaccggga agatgggccg
      721 catacagtgc aaacaccgga agaatcagga acatgcccag catgcgcaaa ccaaaaatgc
      781 tggccaggcc catgctggcg cgggattctt ccggcgtcat tttttcagaa atatccgaac
      841 tctgtttcat aggcttggga accactgtaa aagtcggtat attagcaggt tagccggcca
      901 cggagtcaac gctttggaca gcatacgcat acgcggtgcc cgcacgcaca acctgaagga
      961 catccatctg gacctgccgc gccaccagct ggtggtcatt accggcctgt ccggttccgg
     1021 caaatcgtcg ctcgctttcg acacgctgta cgccgaaggc caacgccgct atgtcgagtc
     1081 gctctcggct tacgcgcgcc agtttctgca actgatggaa aaacccgacg tcgattcgat
     1141 cgaaggttta tcgcccgcca tttccatcga gcaaaaggcc gccagtcaca acccgcgctc
     1201 gaccgtcggc actgtcaccg aaatccatga ttacctgcgc ctgctgttcg cgcgcgtcgg
     1261 tacgccgcgc tgtccgcaac accagctcga tctggccgca cagacggttt cgcaaatggt
     1321 cgaccatgtg ctcgcattgc cggccgaatc gcgtctgatg attctcgcgc cggtcatcag
     1381 cggccgcaag ggtgcgcagc aggaactgtt cgacgaactg cgcgcacaag gattcgtgcg
     1441 ggtgcgcatc gatggcaccg tgtacgagat cgacgccgtg ccggcactcg acaagcaacg
     1501 caagcacacg gtcgaggtgg tggtcgaccg cctgcgcatc cggcccgaca tgcaacagcg
     1561 gctggcggaa tcgttcgaaa ccgccttgcg tcacgccaac ggccgcgcgc tggccgttga
     1621 tctcgacagc ggcgaagaac acctgttttc cgcccatttt tcctgcccgg tctgcgatta
     1681 ttcgctctcc gaactggaac cgcgcctgtt ttctttcaac aacccggccg gtgcctgccc
     1741 gaaatgcgac ggcctgggca gcatttcgtt tttcgacccc gcgcgggtcg tcgcctttcc
     1801 gcacctgtcg cttgcagccg gcgcaatcaa gggctgggac cggcgcaacc cgttctattt
     1861 ccatttgctg caaagcctgg cgcgccatta cggtttcgac ctcgacgccc catgggaaca
     1921 attgccggaa ggggtgcacc gtgtcctgct gcacggcagc ggcaaggaaa ccctcgcctt
     1981 tcagtacccc gatgaacgtc gcgcgcgcat gcacagtttc gaaggcattc tggccacact
     2041 ggcgcgccgt taccgcgaaa ccgaatcgag cgcactgcgc gaagaactgg cacgctacct
     2101 caacagccag ccctgtccgg agtgcaacgg cgcgcgcctg cgcatcgaag cgcgcaacgt
     2161 gttcgtcgcc ggccactcgc tgcaccagct gtccgccttg ccgctgcgac acagcctggc
     2221 cttcttccag tcgcttgctc ttgccggcaa caaacagacc atcgccgaac gcatcgtgcg
     2281 cgaaatcacc gatcgcctga acttcctgac caatgtcggg ctcgactacc tgtcgctcga
     2341 ccgttcggca gaaacgctgt ccggtggcga ggcccagcgc atccgcctcg cgtcgcagat
     2401 cggctcggga ctgaccggag tcatgtacgt actcgacgaa ccctccatcg ggctgcacca
     2461 gcacgacaac aaccggttgc tcgaaaccct gcgccgcctg cgcgacctgg gtaactcggt
     2521 gatcgtggtc gaacatgatg aagacgcgat ccgcagcgcg gatttcattg tcgacatggg
     2581 gcctggcgcc ggggtacacg gcggccgtgt ggtcgccagc ggcgataccc aggccatcat
     2641 ggcctctgcc gaatcgctga ccggtgccta cctgtccggc cgtcgccgta tcgcgttgcc
     2701 cgccacacgc cgcccgcccg acccggcgcg gcaactggta ctgactggcg cgcacggcaa
     2761 caacctcaag cacgtcacgc tgacgctacc ggcaggattg atgacctgcg tcaccggcgt
     2821 atccggttcg ggcaaatcaa cgctggtcaa cgataccctg tacgcggcaa tggcccgcca
     2881 cctcaatggt tcatcgatcg aaccacaacc cttcgccagc ctgtcgggtg aggacctgtt
     2941 cgacaaggtc atcaacatcg accaaagccc gatcggccgt acgccgcgat caaatccggc
     3001 cacctacacc ggtctgttca caccgatccg tgaactgttc gccggcgtac cggcagcgcg
     3061 cgagcgcggc tacgaaccgg gccggttctc attcaacgtc aagggtgggc gctgcgaagc
     3121 ctgtcagggc gacggtgtgg tcaaggtgga aatgcacttc ctgccggatg tctatgtcac
     3181 ctgcgacgtc tgccagggca agcgctacaa ccgcgaaacg ctcgaagtcc gctaccgcga
     3241 ccactcgatc gctgacatcc tcgacatgac cgtggaaaat gcccagacat ttttcagtgc
     3301 cgtaccgacc atcgcgcgca agctcaacac cctgatgcag gtcggactgg gctacatccg
     3361 tctcggccag cccgccacca cgctgtctgg cggcgaagcg cagcgtgtca aactgtcact
     3421 ggaactgtcg cgacgcgata ccggacgcac actgtacatt ctcgacgaac cgaccaccgg
     3481 cctgcacttc catgacattg ccctgctgct cgaggtgctg caacggttat cgcagcaggg
     3541 caacaccgtg gtcatcatcg aacacaacct cgatgtcatc aagactgccg actggatcgt
     3601 ggacatcggt ccggagggtg gcgaaaacgg cggacgcatc ctgatcgccg gcacgccgga
     3661 acaggtcgcc agccacccgc acagcctgac cggccgttat ctcaaacccc tgctcggatg
     3721 accgccgcca tgtccgtcca ttacaccctg accgacctga aaagcacgtt cccgccagca
     3781 tttttcgaac gggcacgttc catccacgaa cagcagcgta tccgccacat ccgttatgag
     3841 gatgacccgc acgtctggct gtcggaagtc gtcggcagcc ggcccttgcc ttaccggcag
     3901 cgtatccagc tcaacctgcg caacggcaag ctgatcattg acggttcgtg tgactgtccg
     3961 atcggccgca actgcaaaca tgtcattgcc gttctcatgc atgcgctgca atacccaccg
     4021 caagcctgca acaggcagca acagcagacc gaaacatctg ccgtccccgg ctccccggca
     4081 ggcagcaaca gcggcagcga tctggcggcg cgcaactggc tgcatcaggt gcagcagatc
     4141 gcgcaacgcc tgcgcgaacc tgtcgtggaa agcgaagagg tcaaccggtt gctctatgtg
     4201 ctgagtccga atccgcatga cggactcgac gtacgcctga tgcgtgctcg ggtcaaaaaa
     4261 gacctcacgc tgggcaaggc catgcgctac acccagtttt tcgaaatcgc actcggacgt
     4321 ggcggcagcc tcgccagcag catcgaccgc acgctgtgcg cccgcatcct gcagtgccac
     4381 ccgcatgctc ctgaatggcc cggtatcagc ggcagcggca gcggcgaact gctacgcgat
     4441 ctgatcgcca gcggacgcgc ctactggaca gaccccgaca ccaccccgct gtcgcttgcc
     4501 gatcccgtcg actgcgagct cgactggatt gccgacgacc gcgacaatca gcaacttggc
     4561 atcgcactgc agccgcacga gcgcctgctg ctggtcgaac cgccatggcg actcgacacg
     4621 cacagcggcc agtgcagcga actgcacacc ggccttccgg atgccctggc gagcgcattg
     4681 ctgcgttcgc caagcattcc caacgacctg ctgcccggca tcgccggcaa cctgcagcac
     4741 caactgcccg aattctctct gcccacgcct cagtccttgc aggtcaatca cctcgaagac
     4801 acacgaccgc tggcgcgcct ggtgctgcgc gaactaccgg tgcatttcgg tgcacactgg
     4861 aagcgccgcg acgaaaacga aacccaccgc atcccggttg cgcaggtcga attctcctat
     4921 cacgggctga cgacattgca tggacagcca ggcgaacagg tcagccagcg caacggactg
     4981 acgcgctcgg tcgcgcgccg cgacctgtcc gaagagcagc gtctgctccg tcaattgctg
     5041 gacggcaccg acctgctgcc ggttcaccag ctgtatccgg cgctcgccgc caccggcgac
     5101 gtgcatgccg cattcggtct ggcgcacact tccgattggc tgacgctgat gatcgacgat
     5161 atcccggcat tgcgtcagct cggctggcag gtgcgcatcg aggatggctt tccttaccag
     5221 attgccgatg cgcaaggcga tttcagcatt gaaatcgacc agccttccgg cagcgactgg
     5281 ttcgatctcg atctcggcat cgaagtggac ggcgaacgca tcagcctgct gcccatcctg
     5341 agccagacat ttcagcaata ttccccggaa cagatccgcg aactgacccg accagacacc
     5401 gagcccaatc gcaaactggt gtgcatgctc cccgacggcc gcatgctcgg actgccgctt
     5461 gcccgcttgc agggcatcct gtcggtactg gtcgaactgt tcgacagcag cacgcgcttc
     5521 aacgccgaag gcaagctgga actgcacgcg ctgcacgcct cgcggctcgc tgaactggag
     5581 gccattgacc aaccgcgctg gctcggcggc gagcgtttga tcgaactggg gcgcaagctg
     5641 cattccttca gcggtatcgc cagtgtgcca ccgcccccgg gtctggcggc cacgttgcgc
     5701 ggctaccagc aacaggggct cgactggctg cagttcctgc gcgaatacgg tctggccggc
     5761 attctggccg acgacatggg cctgggcaag acgctgcaga cactcgcgca cctgctggtg
     5821 gaaaagcact ccggccgcgc cgacctgccc agtctggtcg tcgccccgac cagcctgatg
     5881 ttcaactggc gcgccgaagc cgcccgcttc gcccccgatc tgcgcgtgct ggtgctgcac
     5941 ggcaacgacc gcaagcagca cttcgaccag atccgcgaat atgacctgat cctcaccacc
     6001 tacccgctgc tgccgcgcga ccgtgaattt ctcacggcac agcgttttca cctgctgatt
     6061 ctggacgagg cgcacgccat caagaatccc aaggccctgt cgacccagat cgtgcaccag
     6121 atccacgctc gccatcggct cgcgttgacg ggcaccccca tggaaaacca tctgggcgaa
     6181 ctgtggtcac tgttccattt cctgctgcca ggactcctcg gcagcgcaga gacattcaaa
     6241 cgccagttcc gcaccccgat cgaaaaacac gatgatccgg cacggcatcg ggcactcatc
     6301 cagcgcatcc gcccattcct gttgcgacgc accaaggcgc aggtggccac cgagctgccg
     6361 cccaagaccg aaatcgtgcg cagctgcgaa ctcggcggcg accagcgcga tctgtacgaa
     6421 tccgtgcgca gtgtcatgca tgacaagatc cgccgcgaaa tcgacgccaa aggttttcgc
     6481 aagagccaga tcgtcattct cgacgccctg ctcaaactgc ggcaggtctg ctgcgacccg
     6541 cgcctgctca agttacctgc cgcacgacgc gtgcaacatt ccgccaagct ggacatgctg
     6601 accgaactgt tgccggaaat gatcgaagaa ggtcgacgca tactgctgtt ctcccagttc
     6661 accagcatgc tcgacctgat cgaacccgaa ctgcacctgt tgaaaatccc ctatgtccgc
     6721 ctgacgggcg agacccagga ccgcgcccaa ccggtcacac gctttcagaa cggcgaggta
     6781 ccgctgtttc tcgtcagtct caaggctggc ggggtgggac tcaacctcac ggctgcggac
     6841 acggtgatcc attacgaccc gtggtggaac ccggcggtgg aagaccaggc caccggtcgc
     6901 gcgcaccgca tcggtcaggc aaacccggta tttgcctgga aactgaccac ggtgggcacg
     6961 gtggaagaaa aaatcgtcgc catgcaggaa agaaaacgca gcctcgccca gggtgtgctc
     7021 ggcgatgccg ataccggcgt gagcgccttt acccccgacg aactcatcaa cctgttcgaa
     7081 cccggttgat gcagcaacct cacgggatgc ggcatcaacc gattggtatg aacaggctat
     7141 tgcctgcaaa acagcaacat atgacaatcc cccacacaca tacaattatt ccagggggaa
     7201 catcatgcag aaacaccttg ctgccagcca cggcctgcgt acatggggag tggcgttcgc
     7261 gctgcttgcg gccaccacgg cccacgccag taccgtcaat cttgatttct ccacttatgc
     7321 tcccgatacg gccatccaga gcctgtccgg catcaccttt tcactcactg gaaacggtgg
     7381 cccgttcaac gcgccggtca ccggccttac gggagaagga ctggccaatg ccgacgcatt
     7441 ttctccggac tacggttttt ccacaccagc tgacaatctc gtcgccacgt tcggttcgct
     7501 ggccagcaac gtcagcttca tctttgacaa tattggcaca agcgcatcga gttataccgc
     7561 ctatgatcag aacggccacg ccatagtctc gggatctctc ggcaacgacc agggcaatac
     7621 cctgaccacg gtggccgcct cgggcatcca tgccatcgta ttcaacaacg gcagcggcaa
     7681 tggcccgtcc aacgactggc tgttcacgct gtacagcctc aacgccgacc tgacacagaa
     7741 cggtttgccg gccggcatcc cgactgaccc gccggccgat ccaccggctg cgcccctgcc
     7801 ggcgtcgctg ccgctcatgc tgtccggact cggcatgctt ggctacgccg gacggcgcac
     7861 acgcaacgcc acgcactgat cctcatgtcg gcaggcggcc cggctcaggc cgtctgcgca
     7921 tcgatcgtga atcccgcccc ggcatcctgg ccaagcacac gtgccaggca gggcatcacc
     7981 gcgcgcagcc cggcttcgag cgtccatggc ggatgcagca cgaacatgcc gctgccatac
     8041 atgccgaatc cgtcggcagc cggtcgacac acctgcagcc ggacgtcgag ccaatcctcg
     8101 ccaagccgtt tcagcgactc gaccatgcgc agcggatcgg tgcgtgacag caacggatac
     8161 cagaccacat agattccggt ggcgaagcgc tgcaaggcat cgcgtacggt ctgcggcacg
     8221 cgcacgtaat cctgcttgtc ctcataggac gggtcgatca gcaccagccc gcgccgcggc
     8281 ggtggcggca acagcgcacg caggccgctc aagccatcga tctgctcggc acggattttc
     8341 ctgtcgccgg caatatgctg ctgcagcacg gcatggtcgg cagggtgcaa ctcgaacagg
     8401 cgcagccggt catcggtgcg caagcgcgag cgggcaatca atggcgaacc cggatacgcc
     8461 tgcagcaccc cggacgggtt gaagctgcgc accagcgcaa catagtcacg caacgccccg
     8521 ggcagatcgt cacggtcata cagacgggca atcccgtcca tgaattccgc gtttttctgc
     8581 gcatacccct gatcgagcgc atacatgccc gcaccggcat gcgtatcgat cacccagaat
     8641 ggcttgtcct tgcgccccag gtattcgagc aggtgcaaca acaccacatg cttaagcaca
     8701 tcggcatgat tgccggcgtg aaaagcgtgt cggtaactta gcatgaggtc ctgattccat
     8761 gaaatgtgtg ccgggaagtg tgcaacacaa cgcgccgcac tgcaaatcag tctctgggcg
     8821 caacctgcga ggacacatcc tgttacaacc gggttacaag ctgatacgcc tgtatcgtgg
     8881 ctgacgaact atcctgcaaa cgacaggtta ggaaacgttc catcaatacc cggaactggt
     8941 cgaaacaccc tatatggagc acatcatgca caccctcaag acacccgttc gtattgccat
     9001 gacaaccagc ctgctggcac tcgccctgct cggtggcgct ggcagcgctc gcgcagatgg
     9061 ctactggggc ggctatgtcg ccccctaccc gcccgccgtc gtctggtcac gccctggcat
     9121 caatgtcatt gtgccttatg gcggcccata ttaccgcccg tacccgtacg gctatggcta
     9181 tggctacggt cacgaatggc gcgagcatgc ctggcgcgaa ggcggctggg gcggaggcga
     9241 tggaggctgg cgcggtcgcg aaggcggctg gcgcggtcgc gaaggcgggg gctggcgcgg
     9301 tcgtgactga cccggccgcc acctcggttc cgtgctatac tcgcacagtc caaatcatca
     9361 accgcgggtg ggatgcgcac cacattcggc ctgcgtccag acatgtccgt tgaacccatt
     9421 ccacttgaag ccgccgtaca ggccctgcgt ttcgtcctgc cactggatgc cccggcggac
     9481 gtgcagctga gccgtttttt ccgcgaacac cccgccctgg gccagcgtga ccgcgcattc
     9541 atcgccgaac tggtatttgg cgtgctgcga cacctgcgtt cgctggaagc cgcctgcgga
     9601 aaatccactc cgcgcctgct ggccatcgcc tggctggcgc gcatccaggg atggacgcta
     9661 cgccagtttg aaccactgct gcgcgcaccc gaactggaac aggtgcccgt catcaagtca
     9721 ctgcaactgg cagaacagcc gcttgccgtg cagtgggatc tgcccgactg gctggcagac
     9781 aagttgctcg cacgtcatgg tgaagccggt gctgcgcggc tggcacaagc tctgcgccag
     9841 ccggcgccgc tcgatctgcg cgtcaacacg caactcacca gccgcgacgc tgcgctggaa
     9901 gccctgcgca ccgagggtct gcgtgtgacc cccaccccat attcgcccac cggcatccgt
     9961 gtcgaaggca agcccgccat ccagcacagc ccggtgttca tgtcgggaca ggtcgaggtg
    10021 caggacgaag gcagccaact gctcggctgg ctgctggcac cacggcgcgg cgaaatggtg
    10081 gtcgacttct gtgccggcag cggcggcaag acactggcac tcggcgccat gatgcattcc
    10141 accggccggc tgtatgcctt tgacgtttcg gagaaacgcc tgtcacggct caagccacg
//
LOCUS       DAIANL010000075        24519 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1317296, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000075 DAIANL010000000
VERSION     DAIANL010000075.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 24519)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24519)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24519
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317296"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5147 a         8128 c         6984 g         4260 t
ORIGIN      
        1 gcgaccgccg aaagcatgaa atccggcgtg gagctcacac tcaggcaact caagtcggtt
       61 tttgacaagt tcaatttgaa agaaatcgac ccggtcggcc agaagttcga cccgcaccgg
      121 caccaggcca tcagcatgat cgacagcacg gccgaaccca ataccgtggt ttcagtgctg
      181 caaaaagggt acctgctgca cgaacgtaca ttgcgcccgg cgctggtaac cgtcgcccgg
      241 gcaaaaaact gaagcgtgtg acttgaattt gactgaccac gacacatata gcgctcatct
      301 cggattactg aactgcaagg actgaaaatg ggaaaaatca tcggcatcga cctcggcacc
      361 accaactcct gcgtctccat catggaaaat ggtgcagcca aggtcattga aaacgcagaa
      421 ggcgcccgta ccacgccttc gatcgtcgct tacaccgaag acggcgagat cctggtcggc
      481 gccgctgcca agcgccaggc ggtcaccaac ccgaaaaaca ccctgttcgc agtcaagcgc
      541 ctgatcggtc gccgctttga agaaaaagaa gtgcagaagg acatcaacct gatgccctac
      601 acgatcgtgc gcgccgacaa cggcgatgcc tgggtggaag tgcgcgaccg caagatgtcg
      661 ccgccggaag tgtccgcgca ggtgttgatc aagatgaaga agaccgccga agactatctg
      721 ggcgagccgg tgaccgaggc cgtgatcacc gttccggcct atttcaacga cagccagcgt
      781 caggcaacca aggacgccgg ccgcatcgcc ggcctcgaag tcaagcgcat catcaacgag
      841 ccaaccgccg ccgcactcgc cttcggcatg gacaagaaag aaggcgaccg caagatcgct
      901 gtgtatgacc tgggtggcgg aacattcgac atctcgatca tcgacatcgc cgaaatcgaa
      961 ggcgaacacc agttcgaagt catgtccacc aacggcgaca ccttcctcgg cggcgaagac
     1021 ttcgaccagc gcctgatcga ctatctggcc gacgaattcc gcaaggaaca aggcatcgat
     1081 ctgcgcaacg acatgctggc gctgcagcgt ctgaaggaag ctgcagaaaa agccaagatc
     1141 gaactgtcgt cctcgcagca aacggaagtc aatctgccct acatcactgc tgacgcaagc
     1201 ggccccaagc accttgctgt caagatcacc cgcgccaagt tcgaaagcct ggtggaggaa
     1261 ctcatcgaac gcaccattgc accgtgcaag ctcgccatca aggatgccgg catcagcgtc
     1321 agcgatatcg gcgatgtgat cctggtcggc ggcatgaccc gcatgcccaa agtgcaggaa
     1381 aaggttcgtg aattcttcgg caaggagccg cgtcgcgacg tgaacccgga tgaagccgtg
     1441 gccgtaggcg cctcgatcca gggcggcgtg ctgcaaggcg aagtgaaaga tgtgctgctg
     1501 ctcgatgtca cgcccctgtc gctcggcatc gagaccatgg gcggcgtgat gaccaaactg
     1561 atccagaaga acaccaccat cccgaccaag gcgagccagg tattttccac ggctgatgac
     1621 aaccagaatg cggtgaccat ccatgtgctg cagggcgagc gcgaaatgtc cgccggcaac
     1681 aagagcctcg gccagttcaa cttgaccgat attccgccag ccccgcgtgg catgccgcag
     1741 atcgaagtca ccttcgacat cgacgccaac ggcatcctgc acgtctcggc acgcgacaag
     1801 gcaaccggca aggaaaacaa gatccgcatc caggccagct cggggctgtc cgaagccgaa
     1861 atccagaaca tggtgaagga cgccgaagcg catgccgaag aagaccgtca ggcccgcgaa
     1921 ctggtagaag cacgcaacca gtgcgacaac ctggtgcatg cggcgaagaa gtcgcttgcc
     1981 gaatatggcg acaaggcgcc agctgacgac aaggcccggg tcgagaccgc cattcgtgag
     2041 gccgaagaag ccatcaaggg caatgaccgc ggtgcaatcg aagccagatc gcaggcactg
     2101 accgaggcca gcatgaagct cggcgagcac atgcatgccg cgacacagca ggaagctgcg
     2161 cagacgggtg cccagggtgc cggggccccg gatgatgtgg tggatgccga gttcgaggaa
     2221 gtcaaggaaa agaaataatt ccggcaggct gactcccagc cggtttgagg ggcggcttgc
     2281 gcaggcaggc tgcccctgtc cgttcattca gcaggcaaat tatcaagacc atggccaaac
     2341 gcgattatta cgaagtgctc ggaatcaacc gggacgccag cgaagacgag gtgaaaaaag
     2401 cctatcgtcg cctggcaatg aagcaccatc cggatcgcaa cccggacaat cccaaggcgg
     2461 aagaactgtt caaggaagcc aaggaagcct acgagacgct ctccgacagc cagaaacggg
     2521 ccgcctatga tcagtatggt cacgcaggtg tcgatcccgg cgcgggcatg ggtggcgccg
     2581 gtggcttcag cgatgctttc agcgacatct tcggcgatat cttcggcggc ggtacccgat
     2641 cgcgcagcaa cgtctatcgc ggggcggact tgcagtacaa cctcgagatc agtctcgagg
     2701 aagccgcccg cggcacggaa acccgcattc gcgtgcctgc ggtggaggaa tgcggcacct
     2761 gccacggcag cggcgcccgc cccggcaccg atgccgtcac ctgccccact tgtggcggcc
     2821 acggccaggt gcgcatgcag cagggctttt tttcgctgca gcagacctgt cccaagtgcc
     2881 acggcaccgg caagatcatt cccagtccat gcaccgagtg ccatggctcg ggacgcgtgc
     2941 gccgacacaa gacgctgtcg gttcacatcc ccgctggcgt tgacaacggc gaccgcatcc
     3001 gcctgtcggg agaaggcgaa ccggggcaga atcatggccc gaacggcgat ctgtacgtag
     3061 tgatccacct caagccgcat gcggtattcc agcgcgacca caacgacctg cactgcgaaa
     3121 tgccggtcag cttcaccacc gccgcactgg gcggagaaat cgagattccc acgctggatg
     3181 gtcacgccaa gatcaagatt cccgccgaaa cccagaccgg aaaagtgttc cgtctgcgcg
     3241 gcaaaggcat tcagggcgta cgctcacatg cgcccggtga cctgctgtgt cacgtgatga
     3301 tcgaaacccc ggtcaacctg actgaccggc agaaggaact gctgcgcgaa ctggaagcca
     3361 tcaatctgac tgatgacgcc cgtcacagcc cgaaggccaa atcgttcatg gaaaaagtcc
     3421 gggcattctt caactgaccg aatcgcgggc ccggccggtg gccgggcctc gaccggttca
     3481 gcgagagaaa tggatcacca cgccgccagc atcgatttcg atgcgctccg gctgaacgct
     3541 gctgcccagc aatttcagat cgcccggggt gaaccggtag accggcatgc cgtttagccg
     3601 ttcgttcatg gcgacggaca gcgcttgcga caacaggacg ttcatcgcat ccgacatgcc
     3661 atcgaaatgc tggctgtcca gttgcggatg gtcaagcacg atcgtcttgt ccgccgcatg
     3721 ccagaccagc gcgctggaca cctgtgtatg cccggaaaac gggcggtcag agccaacgag
     3781 gcggatcagc gtatcgaact gcagagtgac gcgctgctgt tgcggcaaca cgcccaccag
     3841 cggatgcgac aacgtcacgt cgaccagtcc ggaataatgc ttttccagcg ggaacctgcg
     3901 ttcgagcgcc tgctgcatct gtccggcact gacctctgcg ctcgatggca gcagccagtc
     3961 agcgcagccg cccaacagca gcaataccgc ggcagccgca accagacgcc gcacgagggc
     4021 gacgctcagc atgccaccac ctcgtcggca tggcccgatt gcttcgccgc agcaaacaga
     4081 cggaagaaat tctcgctggt gatccgtccg gtctcagcca catcctcgcc gcgcaggcgg
     4141 gacagttcct gcgccacatg tactacccac gcaggctggt tggtcttgcc gcggtgcggt
     4201 actggcgcca ggtaaggtga atccgtttcg atcagcaagc gatcggtggg caccatgcgc
     4261 accacgtcgc gcagcgcttc cgcattgcgg aaggtgacga tgccggaaaa agaaatgtaa
     4321 aaccccaggt cgagcgcagc ctgcgccacc tcggcgctct cggtaaaaca gtgcatcaca
     4381 ccgcccacac gatgcgctcc ttcttcgcgc aaaatacgca gcgtgtccgc ggcggccgcc
     4441 cgcgtgtgca caataagcgg tttgccactc gctaacgccg cgcggatgtg cacgcggaaa
     4501 cgctcctgct gccactccgg cacgccaggc tggcggaaat aatccagtcc ggtctcgccg
     4561 atcgccacca cacggggatg cgctgccagc tgcgtcagtc tctcgacatc gggctcatcg
     4621 acgccctcgt aatcgggatg cacacccacc gaagcagaca ggttgtcatg acgctccacc
     4681 agcgccagca ggcgcggcag atcttccagg cgaacacaca cgcacagcgc atggctgaca
     4741 ccggcgtcgc gcatcgctgc cagcgcttcc gcctcgtgcg ctgcgagatc gggaaaatca
     4801 aggtgacagt gggaatcgac aaacatgcgc ggtcagatgg tgtgggtggg acgggcagcc
     4861 ttcagcgcgc caccgagcaa cccctcgatg cggttgcgcg ccgccacgcc attttcgttg
     4921 tcgtcgaact gtacgccgat acccggtgtc ttgccgccat gacagccaac cggggtgatc
     4981 cacaccacct tgccggcaac cggaatcttg tttgggtctt cgagcaatgt cagcagcatg
     5041 aacacctcgt cgcccaactg gtaggaacgg gtggaaggaa tgaacaggcc acctcccttg
     5101 atgaacggca tgtaggcagc aaacagcgcg gatttttcct tgatcgccag cgacagtacg
     5161 ccgggacgcg ccatatttgg aatcgaactc atattctccc ctcacttgac gggccattga
     5221 tcaacgttgc tgacaatgcc cggacaagac gcaaccgggg gaaaacgcag ttgatctgct
     5281 gtaaaccgcc aggttgacgc tgcccgcacc acattgcctg atgggccgta ttcacccctg
     5341 cgcctcaaac agggcacaat aatcgagcaa aatatcctcg accgccagtt gcacgttgag
     5401 cggatgggcg ctcgtgcgac gcacttccgt cagccgctgg gcaaatcgca ccaggccgtg
     5461 cacatcggca cgcggagcaa gcgactgcag atcggcggcg caatcgccat gataaccgat
     5521 tctgccgacc atgcgcaggg atagcaaatc gtacatccag cgctgcaaca catcgatcca
     5581 gtcgagataa gcctcgtgct ccatgcgcgc cagctgctcg gcctcattcc agacgcgcgc
     5641 gctgtcggcg cgcgccagca tctccagcca gcccgcacgg cgcgccatgc gttcgacatc
     5701 attccacgcc agcgcagcca gcggtgcccc accggcaaga tcgagacaca gtcctggctg
     5761 agccactccc tgacctgcaa gccattccag cgcaccggca cggtccggca acgggtgacg
     5821 cacggcatgg caacggctca gcaccgtcgg cagcagtcgc gccggctgat cggtgaccag
     5881 cagcaacaag gtgcggtccg gaggctcttc cagcgtcttc agcaaggcat tggacgcagc
     5941 cacattcatg acatgcgcct gatggatcca caccacacgc atcggaccat gggcaccgat
     6001 gcgcaggaag tcgacggcct ggcggatcga atccacaccg atctgcgcca gcttgcgtcc
     6061 cggggtcgcc tcaccgccct cctccgcggc agccacatcc acctcaagca catccgggtg
     6121 ctgggcagcc agcaaccagc ggcaggaatc gcactgaccg caggcctgct ctgccgtggg
     6181 atgcgcgcac agcagccatt gcaggaaggc gccagcaagg gcgcgcttgc cgccgccagc
     6241 agggccctgc agcaacaccg catgcggcag acgcgtgcgc atggcgcgca gctgacgcac
     6301 gggctcactt tgccacggat acggctgatg catgattaca cctgccacca ggatgcaaac
     6361 atgtgcagca actcggtacg aatggcctcc aacgtacggt cggtgcggat cacccggatg
     6421 cgccgtggtt cggcctgtgc ccgttgcagg tacacggcac gcacgcgttc gaaaaacgcc
     6481 tgctgttcgc gctcgaatcg gtcaggctca ccttgtgcag cacggcgttc gcgcgcaatc
     6541 tccaggtcaa cgtcgaacag cagcgtgatg tccggctgca atccctgctg cacccaggtt
     6601 tccagaatac gcaaacggtt ttcggcgatg ccacgtccgc cgcactggta ggcatagctg
     6661 gcatcggtaa aacggtcgga caggacccac tcgcctcgcg ccagcgccgg acggatcacc
     6721 tgtgcaagat gctcggcgcg ggcagcaaac atcagcagca attcggtgtc agcactcatg
     6781 gtctggttga gcagcacgga gcgtaatgcc tcgcccagcg gggttccacc cggttcacgc
     6841 gtcaccgtaa ccgtcttgcc atgcgcgcgc aactgctcgg ccagccagtt cacatgcgtg
     6901 gattttccgg cgccgtcgac gccttcgaaa ctgacaaatg gcatgccagt gcgttccttc
     6961 ccctgttatc gtaaacttcc ttcatcgccg ccatacccgc acacggcagg ccgtgtggcg
     7021 tcagcgcaat ttctggtagc gcgccacggc ccggttgtga tcggcaagcc gtgccgaaaa
     7081 aacatgcgta ccatcgccac gcgcaacgaa atacagatca ttgctcgcga gtggatgcaa
     7141 cgcagcctcg atggaggcca tgcccggcat cgcaatcggc ttcggcggca aaccggcacg
     7201 cgtgtaggta ttgtacggcg tatcggtctg caagtcgttc ttgcgcagat tgccatcaaa
     7261 ggccgaaccc atgccgtaga tcacggtcgg gtcggtctgc aggcgcatac ccaggtgcaa
     7321 ccggttgatg aacaccgaag ccaccagcgg tcgctcgctg gcctgcccgg tttccttctc
     7381 gatgatcgat gccatggtca gcgcctgcac cggcgatgca tagggcagcc cggacgcacg
     7441 tcctgcccag gcctgctgca ggtgctgctg catcagcgca taggcgcgtc gcagcactga
     7501 caggtcactg ccgccaacgg tcacgtcgta ggttgagggg aaaaaccagc cctccggtga
     7561 tgcctgatgc atgtcgagcg cgcgcatcac ctctgcatcg ctcatgcccg cgctgtcgtg
     7621 gcgtatccac gggttggcat cgagtgcggc gcggatctgg gcaaacgtcc atccttcgat
     7681 gaaacgcacc tgcagcggcg tgacctcgcc attggccagc ttgtgcagca gttgcaacgg
     7741 cgtgacaggg gtctgcagca cgtacaatcc agccttgagc cgattggcat cgccactggc
     7801 aaacgccagc aaccgaaatt cgagcgcatc gagatgaccc tccggcaagg cgccggcatg
     7861 ctgcagttga cgggtgatca ccccaagccc ggagccaggc atgaccacga cgctggcctg
     7921 gccatgctgc aatggcacgg cactggaagc aaagcggtac acctgtgctg tcgcaagcgc
     7981 ggccatgccg aaaatcaatc ctgccaacag agtcctaatc atcttccctt tccagcaagc
     8041 cacgcaaacg cggagtggcc gtacctgccg gccagcgccg gtgcgccagt tcgcgcacct
     8101 gccatatgcc gatcagactg ttgcacaaca tgacttcatc cgcatcgagc acatcctgca
     8161 atgtcagtct ggcactgcgc gccgtgtaac ctgcccgatg tgccagcgtc aacaggcgcg
     8221 ctcgcgtcac tcccgctacg ccactgccct caatttcagg cgtcaacaat tcctgttgcc
     8281 gcaacaggaa cacattgctc atcgtaccgc ccgtcaggtg gtcgcgctgg tcgaacaaca
     8341 ggccttccgc aatggccgga tcacgccact ccgaccgcgc cagcacgttt tccagccggt
     8401 tgagatgctt gattcccgcc agttgcggct gccaggccag tcgcaggtcg cagacccgca
     8461 cccgcacacc ctcgatacga tgaccgggtg gatactccgg caacgggctg cgcagcacca
     8521 cccgcgtcgg ttcggccccg gcaaccggcg catagccgcg tgcgctttcg ccgcgggtga
     8581 cgatcagctt gatgaccgca tcctgctcgc cgcgcgccag ttccgcaatc tcgcgttgca
     8641 acagcatccc ggccggaggt tcgatgccaa tgcgtgcgca gtcaccagcc agcttggcat
     8701 actgtcgtga ccactgcaat accgttcccg cacgcagtct cagggtacgg aacacgccat
     8761 cgccatacgc aaggccgcga tccagtgcgg aaacacagcc ccattcctga ccattgacca
     8821 gaatcacgat gctgcaccaa acgcacgcag catgccgcga accttggcac gcgtctccgc
     8881 cagttcgcgc tgcggttgcg aatcagccac gataccggcc ccagccgaaa actctgcccg
     8941 acggtcatcg atcatggcac tgcgtatcag aatattgaga tcaagatcac catcgatgtt
     9001 cacataaccc atacttccag tgtaggcatt gcgcgcctgc ggctcgagtt cccggatgat
     9061 ctgcatggtc ctgaccttgg gacaccctgt gatggtaccg ccggggaaca gcgctcgcag
     9121 caacgccaca ggagttacgc caggctgcaa ccggccggat accgtggatt cgatatggtg
     9181 cacccaggcg tagctcgcca cgcccatcaa ttcctccacc cgtacgctcc cgggcaggca
     9241 gacacggccg aggtcattgc gttccagatc gaccagcatg acatgttcag cgcgttcctt
     9301 gggatgcgcc agcaactgtt cgcgctgtag cgcatcgcgt aacggatcgg gatctctcgg
     9361 ccaggtgccg gcaatcggtc gcgtgagtgc ccggccagcc ttgacgcgca acaaccgttc
     9421 cggcgatgcg ctgacgatgg actggcgctc accaagtgaa gcgataccgc caaacggcac
     9481 cgggtttccg gctcgcaacg tgcggtacaa ctgcgctgca cttgcctgtg acgtcaaccg
     9541 cgcatgccag cgacgtgaca gattgacctg gaatacatca ccggcacgga tatagtcctg
     9601 caccgtggcc acgttatcca ggaagatctg ttcgtcttct tcttgcagcg attcgagcaa
     9661 cgccgcagcg ctaccgctgg ccgcaggcgt ggccgcgatg gccgcccgca gttcgtgcag
     9721 actgtgcctg ctttcggcaa acagccacgc ctggccgcgc ctgcgatcaa tctggatcgc
     9781 tgccggaatg cgcatgcgta ccgcaagcgg gaaggcaggc tgctcatccc gtgccaccac
     9841 ggacggttcc agcacatgca gcatctcgta gccaaggtag acgaaccagc caccgcgaaa
     9901 cggcacgtgc gccgcatcgg caacagcatc cggccgttcg cgccattccg tctcgaaccg
     9961 ggcaaggaag gtggcacctt caccggcatc agcgcgatgc acctgttgcg gcagcgccag
    10021 cagaatatcc cagccatcgc cgctgcccgt ttcaagcagc accggaaact gctgcggatc
    10081 cgcctcatgc agcgcaagca gatccggcaa ggcagggagc gccagggtag gcactactac
    10141 acgcgacgga acaccagtgt gccgttggtt ccgccaaaac cgaacgaatt ggacaatgcc
    10201 aggtcgattt tcatctcgcg ggcagtgttc ggaacataat ccaggtcaca ggcttcgtcc
    10261 tggtcatgca ggttgatggt cggcggagcc acctggtgat acacggccag cgtggtataa
    10321 atggcctcga caccaccggc ggcacccaac aggtggccgg tcatcgattt ggtcgaactg
    10381 atggccgcct tgcgcgcgtg ctcgcccagg gcaagtttga gtgccgcagt ttcggccaga
    10441 tcgcccagcg gcgtcgacgt gccatgggca ttgatgtatt cgatctggtc ggcgttgacc
    10501 ccggcattgc gcagcgcatt gcgcatgcag cgcgcggcgc cttcgccgtt ctcgatcggc
    10561 gcggtcatgt gataggcatc gccgctcatg ccaaaaccgg ccagctcggc atagatgcgc
    10621 gcgccacgcg cgcgcgcatg ttcgtattcc tcgagcacca tgacaccggc gccttcgccc
    10681 atgacgaatc cgtcgcgctc gcgatcccac ggacggctgg cgccctcggg atcatcattg
    10741 cgcgtcgaca gcgcccgtgc agctgaaaat ccgcccatgc ccaacgggct gatggccgat
    10801 tccgcaccac cggcgatcat cacgtccacg tcgccgtact cgatcaggcg ggcggaatcg
    10861 ccgatggcgt gtgtggccgt ggcacacgcc gtcaccatcg ccagattcgg acccttgagc
    10921 ccgtacagaa tcgacaggtt gccggaaatc atgttgatga tcgacgccgg tatgaaaaac
    10981 ggcgagatct tgcgcggacc gctttgcaga taaacactgt gcgtttcctc gatcatcggc
    11041 aacccgccga tccccgagcc cacactcacg ccgatgcgct cggcattggc ctcagtgact
    11101 tccagccccg aatcacggat ggcctcgatc ccggccgcca tgccgtactg gatgaacaga
    11161 tccatacggc gcgcttcctt gggattgaga aacttcgaca cgtcaaaatc acgcacttcc
    11221 ccggcgattt gcgaggcaaa tggcgacgga tcgaagcggg taatccgact gatgccggag
    11281 accccggcga gaatgttggc ccaactggtg gcgatcccgg taccgaccgg ggatacaatc
    11341 cccaacccgg taatgactac tctgcgtctg gacaagagtg ctccacgaat gactgaagat
    11401 gattatttgg caagatgcgc attgacgtag tcgatggcct gttgcacggt ggtgatcttt
    11461 tctgcttctt cgtccggaat ttcgcactcg aattcttctt ccagcgccat caccagctcg
    11521 accgtgtcca gcgaatccgc tcccagatcg ccggcgaaag aggactcggg cttgatttca
    11581 gcctcgttga cgcccagttg ctcagcgacg attttcttga cacgctgctc gatgttttcc
    11641 atgtatgaca ctcccgattg ataaaaaagc cccgctattc taacaaagtc ggccaagatc
    11701 accaaaggaa acactgattt attcgccgga caacaggcaa ggcgaagcac ccggctcaca
    11761 gacatcgcga catatcaaat agatagtcgg aatggcaagc gccacgccgc gcatcatcac
    11821 gctcgcgcag tcaggcagtc agcatttcca caacggctcc tggccgtgcc acagttactc
    11881 catgtacatg ccgccgttca catgcatcgt cgcaccggtc acataacctg ctccggccga
    11941 tgccaggtag gctactgcat gcgcaatgtc atccaccgcg ccgagccggc ccagcggtat
    12001 ctgcgcgagc aggcccgtac gcactgcgtc aggcagcgcc cgcgtcatgt cagtatcgat
    12061 gaatccgggg gcaacgcagt tcacggtaat gttgcggcta ccgacctcgc gcgccaacga
    12121 tttggaaaaa ccgatcatgc cagccttggc ggctgcatag ttggtctgac cggcattgcc
    12181 ggacgccgcc accaccgagg caatattgat gatgcggcca taacgcgcct tcatcatgcc
    12241 gcgcaacact gcgcgagaca ggcgaaacac cgagttcaga ttggtatcga tcacctggtt
    12301 ccagtcatcg tccttcatgc gcatgagcag gtcatcgcga gtaatgccgg cattgttgac
    12361 cagaaccgcc acttcacccg cctgctggcg gacggtttcc agcacggcct cgatggcctc
    12421 cggctgcgtc acatcgagca tcagcccctg gccgtccagt ccggcgtcgc ggaaataatc
    12481 gccaatcgcc gcagcacccg aagcgctggt cgcggtaccg aacacccgcg cgccctggcg
    12541 cgccagtgtc agtgcaatgg cctggccgat accccggctg gcaccggtca ccagtacggt
    12601 cttcccttgt ggcatgtctt tggtctcccc gaatcagtcg taatcgttca cgcggccaga
    12661 agctcattca cgttgtccgc gaaagcgcgc aacgcatcac tgctgatcaa cgcctgggta
    12721 ggcacgcgtt cgtcgatgcg tctggccagc ccggccagca ctttccctgg cccgcactcg
    12781 cccgccaggc gaataccggc atctcccatc gcccgcacag tctccaccca gcgaaccggg
    12841 ctgtacagtt gacgcgacag cgcatcacgg atggctgcgg catccccgta gaccgccacg
    12901 tcggcattgt gcagcaccgg gacaatgggc accgcgatcc cgaccttttc cagttcatcg
    12961 gcgagcaccc gggccgccgg ctgcatcagc gcgcagtgcg acggcacgga caccggcaac
    13021 agcacggcac gcttggcacc acgttccttc gccagcagca ttgcccgttc cacggcaagc
    13081 cggtttccgg caatcacgac ctgaccatcg gcattgtagt taactgcctc gagcaccagg
    13141 tcttctgctg catcgcggca cacaccgcgc accgcatcgt cgtccatgcc cagaatcgct
    13201 gccatggcgc cggtaccttc gggcacggcg tcctgcatca gttgcgcacg cagacgcacc
    13261 agtcgcagcg cgtcggcgaa ttcaagcgcg cctgcggcga ccagggcggt atattcaccc
    13321 agactgtgtc cagccatcat ggccggcaat ggcccgccca tcgcccgcca cgcacgccag
    13381 gtggccacac cggcggccag catgaccggt tgcgtattca ccgtcagatt cagcgtgtcc
    13441 tggctgccct gttcgaccat ctgccacagg tcttgcccaa gcacttcggt ggcctcatca
    13501 aacgtttcgc gtacaccggc cagttcggcc acgccctgca tcatgccggg cgtctgcgaa
    13561 ccctgaccgg ggaacaccaa agcagacatc gggttcgaca tcgtcattcc tccgtcatcc
    13621 atcgcaccag cgcagagccc caggtgaagc caccgccaaa tgcttccatc aaaatcagtt
    13681 cacccggacg gatgcgtcca tcgcgcaccg cggtatcgag ggcaagcggc accgatgccg
    13741 ccgaggtatt ggcatggcga tcgacggtaa gcaccacccg gtccatgctc atgccgagtt
    13801 ttctggctgt cgcctgaatg atccggatgt tggcctgatg cggcaccagc cacgcaatat
    13861 cggatttctg caggtggttg gcctccagcg tctcgtcgac gatgccatcg agcgtgttga
    13921 cggcaaaacg gaacaccgcg ctgccctgca tgcgcaccgc gctcggtgat tgcatcccgt
    13981 gcgacacgcc gccctcgaca tgcagcaggt cgcgataacg tccgtcggca tgcagatgcg
    14041 tggcaatcac cccgggttga ctgcttgccc gcagcaccac agcgccggca ccgtcgcccc
    14101 acaggatgca attgccgcga tcgttccagt cggtaatacg cgacatggtc tcggcaccga
    14161 tgaccagcgc gcagcgcgcc tggccggcgc gaatgaaatt atccgctgtt gccagcgcat
    14221 aaatgaaacc gctacacacc gcctgcacat caaatgcagg gcaatgattc tccagtccca
    14281 gtttcgcctg caccagacag gccgtactgg gaaataccag gtcaggggtg gtcgtggcca
    14341 ccacgatcag gtcgatctgc gacgcctcga ttccggcggc ctcgattgcc cgacgcccgg
    14401 cagccgcagc caggtcgctg gtcatttcac cttcggccgc gatatgccgc tggtggatgc
    14461 ccgtgcgttc cacgatccac tcatggctgg tatcgacgcg cgcggccagt tcatggttgg
    14521 tgaccacgtg ttccggcagg tagccaccgg tgccagcgat tctggaatac ttcatcgttt
    14581 caggctgcat gcttgtgtac cgtttccatt ttttcaccaa tgcgctgcaa tacctggctg
    14641 cgtacttcac gcacagcgcg accgatggcc tgctcgaaag aaaatcgatc ggcaccgcca
    14701 tggctcttga ttaccacgcc gcgcaggcca agcagcgtcg cgccattgta gcggcgtggg
    14761 tcgacccgat gcttgaacgc attgagtacc ggcagcgcca ccacgccagc cagccgggtg
    14821 agcgcattgc ggctgaattc ctcgcgcagc atggtcgaca ccatcttggc caggccttcc
    14881 gaggtcttga gcgccacatt gccgacaaaa ccgtcgcaca ccacgacatc ggtggtacca
    14941 cggtagatgt catcaccttc gatgttgccg tagaaattga gaccactggc gcgcaacagt
    15001 tccgccgcga gcttgacggt ctcgttgccc ttgatttcct cttcaccgat gttgagcaga
    15061 ccgacgctcg gcacttcgca gcgttcgagc gccgatacca gcaccgatcc catgatgcca
    15121 aactgcagca gatgctccgg cgtgcagtca acattggcgc ccagatcgag catgacagtg
    15181 ctgccacgca tggtcggcag ttcactggcg atcgccggac gctcgatgcc gggcagcatc
    15241 ttgagcacga agcgcgcagt agccatcagc gcacccgtat tgccggccga aacacatgcc
    15301 cccgcctcgc cctgcttgac gaggtcgatg gcgatgcgca tggaggaatc cttcttgttg
    15361 cgcatggcca gtgcaggaga ctcgtccatg ccgaccactt cgctggcatg gcagatgcgc
    15421 agacgctcgc cgacctgttg ccggtgaatg cgcaattcat gttctatggg ttcgcgcagc
    15481 cctaccagca ccacattgag ctgcggatcg tgcgccaggc tgtgcagcgc ggccggaaca
    15541 gtcacctggg gaccatggtc cccgcccatg gcgtcgacgg caaccgttac ggattcagtc
    15601 atgtgatgtc aggacgggat tactcgcaaa ccgccgcggc attggccagc ggcggaggaa
    15661 tgaaacagca ctgccggttt cagtcgcctt tggttgcgac cactttgcga ccacgataaa
    15721 acccgttcgg gctgatgtgg tggcgcaggt gcacctcgcc cgtggtcggc tcgaccgcca
    15781 gcggagggtt ggtcaggaag tcgtgtgcgc ggtgcatgcc acgcttggac ggggatttct
    15841 tgttttgttg aacggccatg tttaacgctc cattgagtaa tttgcccggc acgcatgcaa
    15901 caggtaaagc cctgtcgctc aaacgtctgc tatcattgcc gcggcacaga attccgtgtc
    15961 ttgcggacgg tgttccatca cctgttccag caggtgtcgt gccggttgca tcggcagcgg
    16021 aaaccgccac gcctgttccg gcgcaagtcg agcatgatat aatcctcaat cgtttttgtc
    16081 aaaaaccaag gtaagtcaag cgcgtgatca agaaaatact tatcgccaac cggggcgaaa
    16141 tcgccgtgcg catcgttcgc gcctgttccg aactcggcat cgtctccgtg gccatttata
    16201 ctgacgccga ccggcacgcg ctgcacgtga agaaggcgga cgaagcctac aacatcggct
    16261 ccgacccggt gcagggctac ctgaatgcgc acaatatcgt caaccttgcc gtggcaagcg
    16321 gttgcgatgc gctgcacccg ggctacggct tcctgtcgga gaaccccgtg ctggcggaaa
    16381 tctgcgcccg ccgtggcatc cgtttcatcg gcccggatgc cgacgcgatc cgcatgatgg
    16441 gagacaagat cgaagcacgc aaagccatga tccgtgccgg catcccggtc gtgcccggca
    16501 gcgaccacaa cctggagaac gtcggcgagg cgcgcgacat ggccgcgctc atcggttatc
    16561 cggtcatgct caaggccacc aacggcggcg gcggccgcgg tatccgccgc tgcgattccg
    16621 aggccgaact tctcaagaac tacgaccgcg tcgtttccga agcgggcaag gccttcggca
    16681 agccggaagt gtttctcgaa aaatgcgtgg tcaacccgcg ccacatcgaa gttcagatcc
    16741 tggccgacaa gcacggcaac acgctgcacc tgttcgaacg cgactgttcg attcagcgcc
    16801 gcaaccagaa actcatcgag attgcacctt cgccgcagct caccgaagcg cagcgtcagt
    16861 acatcggcaa actggccgtc acggccgcac gcgcggtgag ctacgagaac gccggcacgg
    16921 tcgaattcct gctcgatgcc gacaaccagt tctacttcat ggaaatgaac acccgtctgc
    16981 aggtcgagca caccatcacc gaaaccatca ccggtgtcga catcgtgcag gaacagatcc
    17041 gcattgccag cgacctgccg ctgcaatacc ggcaggaaga catcgagcac cgcgggtatg
    17101 cgatggaatt ccgcatcaac gccgaagacc cgcaaaacgg ttttctgcca agcttcggcc
    17161 gcatcacccg ttactatgct cctggcggcc ccggcgtacg caccgacgca gccatgtaca
    17221 ccggctatgt cattccacct tactatgact cgatgtgcgc caagctcacg gtatggaacc
    17281 tgacctggga aggtgcagtg gaacgcggcc ggcgcgcact gaacgacatg gtggtgttcg
    17341 gcgtcaagac caccatcccc tattacctgg aaattctcgg caatgccgat ttccgcagcg
    17401 gtcatttcga cacctcattc gtcgaaaccc acccggagct ggtacactac gacaccggaa
    17461 agccgccgga gatcctcgcg gctgccatcg cagcggctat cgcagcccac aacggactat
    17521 aaccacacga ctatcgaatt tcagccatgg ccaaagtatt catctccgac ctggtgctgc
    17581 gcgacggcca ccagtccctg atcgccacgc gcatgcgcac cgaacacatg ttgcccatct
    17641 gttccaagct ggacgacatc ggcttctggt cactggaagc ctggggcggc gccacgtttg
    17701 acgcctgcgt acgctttctc aaggaagacc cctgggaacg tctgacgcaa ctgcgccgcg
    17761 ccctgcccaa caccccgatc cagatgctgc tgcgcgggca gaacctgctc ggctaccgac
    17821 actatgccga tgacgtggtg cgcgccttcg tgcacaaggc cgccgacaac ggtgtggatg
    17881 tgttccgcgt cttcgacgcc atgaacgatc tgcgcaacat gcgagtatcg atcgaagccg
    17941 tgctcaaggc cggaaagcac gcccagggcg ccatttccta taccaccagc ccggtacacg
    18001 atatcccgca tttcgtcgaa ctggcgcaag gctttgccga actgggctgc cagaccatcg
    18061 ccatcaagga catggccggt ctgctcacgc cggcgcgggc cggcgaactg gtcagtgcca
    18121 tccgcgcggc aaccggcttg ccggtgcatt gccacagtca cgccacctca ggacttgcca
    18181 gcatggtgca cctcaaggcg gtggaagccg gcgccggcat catcgacacc tgcatttcgg
    18241 cattttccga gggcgcaagc catccgacca ccgaaagcat ggtggcggca ctggccggta
    18301 ccgaatggga taccggtatc caccttggcc agctcgagga aatcgccgcc tattttcgcg
    18361 aagtgcgcaa gctgtactgg caatacgaaa gcagtttcac cggcgtcgat ccgcgtgtac
    18421 tcatcagcca ggtgccgggt ggcatgatct ccaacctgtc gaaccagctc aaggaacagg
    18481 ggtcgctcga ccgcatggac gaagtgctgg cggagattcc gcgcgtacgc gccgatctcg
    18541 gctaccctcc gctggtcacg ccgacttcgc agatcgtcgg cagtcaggcc gtactcaacg
    18601 tactaagcgg tgggcgctac aaaaccatca gcaacgaagt caagctgtac ctgcaaggcc
    18661 gttacggcaa ggcgcctggc acgatcaacc ccgaggtgcg cacgctggcg atcggcgaat
    18721 cgaccgtcat cacctgccgt ccggccgaca tgattccgga cgaaatggac aagctgcgtt
    18781 ccgagatcga aggcgtatcg caatcggaag aagacgtgct cacctacgcc atgttccccg
    18841 acctgggtcg caccttcctg caggaacgca acgccgacac gctcagtccc gaactgctgc
    18901 tgccggtgag cgcgaaaaat gccggcggcg aaagcacccg cttcgctgcg gatgaattcc
    18961 gcgtcacgct gcacggcgaa acctatcaca tccgtctgac cgggactgga cgcggctcgc
    19021 aatccgaacg cccgtactac gtttcggtgg atggtgtttc agaagaagtt ttcgtcgagg
    19081 cgctcaatga aatcgagatc agcggcgaca gcggcagcgg caagccacgc acggcgggca
    19141 aacccgcggc cggaagccgt ccgcggccga cgcacagcgg ccacgttacc acggccatgc
    19201 cgggcaacat cgtcgacgta ctggtcgcca ccggacagcg cgtgctcgct ggcgatccgg
    19261 tgctggtgat cgaagcgatg aagatggaaa acgagatcca ggcttccatt tcgggcacca
    19321 tcatcaacct gttcgtcacc aagggtgatg cggtcacacc cgatcaggcc ctgctcgaaa
    19381 tccagcctga atgagcacca ccgtgcccgc gctggtactc gcgtccacgt cgcgctaccg
    19441 ccgcgaactg ctcgaacggc tggggctacc tttcggcgtc gacgccccca gcgtcgacga
    19501 gagcgtgtct gccggcgaat cgccgcaggc ggctgcgctg cgactttccg cattgaaggc
    19561 tcaagccgtg gcgccccgcc acccgcatgc gctcatcatc ggctcggacc agatcgccga
    19621 actcaacggt cggcacatcg gcaaacccgg tcatcatgct gcggccgtag cgcaattgca
    19681 ggccatgcgc ggccagaccc tgcggtttca caccgggctc agcctgctca ataccgccag
    19741 cggcacaata cacagcgaaa ccgttaccac cgaagtacgc atccgcacgc tcagcgacaa
    19801 tcagatcgaa cactacctgc agcgtgaacg gccctacgac tgtgccggaa gcgccaaatc
    19861 cgaggcgctg ggcatcgccc tcatggaaag cctgcgcggc gatgatccca ctgcactgat
    19921 cggcctgccg ctcatcgcgc tgacccgcat gctcgccagc gaaggggtgg acgtgctgct
    19981 gggctgacgc acgccggatg ccgccctgcg gcccctttca tgaaatctgc tgcgccacgc
    20041 cacgggtcct gcgtggactg tgccgcctcg ctcactcctg ggctgccacc agcgacccgt
    20101 cttccagacg cagaatacgt tgcatgcatc ccgccagttg cacgtcatgc gtcaccacga
    20161 gaaaactggt gccgtgcgtt tcattgaggc gccgcatcag ttcgtgaatc gactgcgcgg
    20221 tatgacggtc gagattgcca gtcggctcgt cagcaagcac gcaggccggt tgggtgacga
    20281 gcgcccgtgc aatggctgcc cgtttgctct cgccacccta cagttcaccc ggcgtgtgcg
    20341 acaaacggtg tgacaggccg acttcctcaa gcatggccgc cgcgatctcg cgtgcctgcg
    20401 catgcggcat gcggcgaata aacaacggca tggccacatt gtcgagcgca gaaaattccg
    20461 ccagcaggtg atgaaactga tagacgaagc ccagcgagcg attgcgcaac acgctgcgcg
    20521 cgcgttcgct caatgcgtgg atatcctgtc cgagcacacg cacttcgccc cgcgtcagcc
    20581 catccagtcc gcccagcaca tgcagcagcg tggacttccc cgatcccgaa gcccccatga
    20641 tcgccaccat ttctccggcc gccaccgaaa aatcgacacc attgagcacc ttgacatcca
    20701 gttggccctg gcggtaatcc ttgtacagac cccggcattc gatcaccgca tcattcatag
    20761 cgcaaagcct ccgcaggttg ggtacgcgcc gcacgccagc tcggatacag cgtcgccaac
    20821 caggacagca gcagtgatac actgccgatc accacgacat cgtgcacatc gagccgggat
    20881 gacagttcgc tgatctggta cacatcggac gaaagaaact gtacaccgaa tgtgtgctcg
    20941 atggccggca ccagccagcc gatattgagc gccagcgcca ccccgccggc caggccggcc
    21001 agcgtgcctg tcacgccgat gacgacaccc tgcagcatga agatacgctg gatactgccc
    21061 ggcgtagcgc ccagcgtgcg caggatggca atatcggcct gcttgtcagt caccaccatc
    21121 accagtgtgg acacaatgtt gaatgcggcc acggcgacga tcagcagcag gatcacgaac
    21181 atgacgtgct tttccatttc caccgcacga aagaaattgc ggtgcgactg gctccagtcg
    21241 cggatgaaaa acgcatcgtc gagactgtgc gacaactggc gcgcgatcag gggcgcctga
    21301 tacagatcgt cgacgcgcaa gcgcagtccg gaaacgcgat cgccggtacg gaacagtttt
    21361 tgcgcatcgg ccatgtcgat cattgccatg cccgcatcgt actcatacat gccgatatgg
    21421 aaaatgccca ccacgcgaaa ttgcttgagc cgggggatca tacccgcagg ggtgatctgg
    21481 ccttgcggcg tgatcagcgt caccttgtca cccagctgca cacccagcag atgcgcaaga
    21541 tcgctgccga tgacgatgcc ccacccgccg ggcacgagat tttccaggcg gccagcctgc
    21601 atgtgccggc cgacatcggc cacaccgttc tccagccgcg gctcaatgcc ccgcaccgcc
    21661 accccctgca ccgcctggtc gtacgccagc aaaccctgcc cggcaacata aggcgcgctg
    21721 gccagtacat gcggattggt gcgacagcgc acggccagcg actgccagtc cgacagcgcg
    21781 ccgtcgaact gactgatctc gatgtgtgcg gccacgccga gaatccgtgt gcgcaattcg
    21841 ttctggaacc cattcatcac tgacaacacc acgatcaatg ccgccacgcc gagcgcaata
    21901 cccagcatcg aaatcaggga aatgaacgaa atgaaatgat tacgccgttt ggcacgggta
    21961 tagcgcaatc caacaaaaaa ctcgaaccag ctcatgatgg taccaaccca aagtccttgc
    22021 ctgccctctc cgcaacgcaa gggctcattt gcccgaacgc cagatggaaa gaatcaccca
    22081 caggctgttg agaaacgcca gcagaaaccc gatgaatccc atgaaccgcc agccgtccac
    22141 ggtcatgacg atagaagaac cgatcaccag cgaagcagtg agaatgccca tggtgatgcg
    22201 gttgctggcg cgatcgatct gctggccgaa cgagtccagc cgcttgaggt cgagatcgat
    22261 ccgtaaccgg ccgcgccgca cttcgcgcat cagccgtgcc atgtcgcgcg gcagccctga
    22321 caccacctcg aatgcctccc tcaggctgcg acgaccgcgt ttcatgatgg cttccggcgt
    22381 gtagcgctgc aggatgacct ggtgcaccag cggcgtcagc tgcgtcgcca gctggaactc
    22441 cgggtcaagc tgctggccaa gcccctccag cgtgaccagc gccttgaaca gcaacgtcag
    22501 gtcagccggc aataccaggg aattgtcgcg gatcaggctg gcgatgtcgt tgagcagcgc
    22561 agcgatgcgc acatccttga gctgcaggtt gtcataattg gtcaacaggt ctgccacgtc
    22621 atgcgcaagc cgcacttcgt tgatctcggc atcgccagtc cagtccatca acacctgcag
    22681 caggccatct tcgttgttct gcgccagcgc atcgagcagg ctcaccagct gctggcggcg
    22741 atattcggtc aggtggccga ccatgccgaa atcaatcagg ccgatgcggt tttccggcag
    22801 ataaatcacg ttgcccggat gcggatccgc atggaaatag ccgtgcacca gaatcatctt
    22861 cagcatcagg tcagcgccac gctgcgccag cagtcgggta tcgagccccg aggcgcgcac
    22921 cgcttccagt tccgaagccg gaatgcccag caattcttcc tgcacattca tcgccgcacc
    22981 ggaatatgcc cagaacaccc gcgggatgtg tacatagtct tcctgggcat actgcttgcg
    23041 gaatcgatcg atatggtgtg cctcgaccat caggtcgagt tcgcgtcgca acgcgcggcg
    23101 gaaatagccg gccatgacca ggggctgata gcgccgggca tcggggaact cgagttcgag
    23161 caaccgggcc agatgctgca ggatgcgcaa gtccgcctcg atcttgcgct ccagtccggg
    23221 acgccgcagc ttgaccacca cgcgcgtacc gtcaagcagt tttgcccggt gtacctgggc
    23281 gatcgacgcg gcagcaaacg gttcgcgctc gaattcggca aacacctcat cgatcggccg
    23341 gccaagttgc gccagaacat ccgcttccac cagttcaaac ggcaccggcg gcacgtgtga
    23401 ctgcaatttt tccagctcgg caatccacgc gggcgacagc atgtccatac gtgtggccag
    23461 caactgaccc agcttgatga acgtcggccc cagttcttcc agcgccagac gaaaacgtac
    23521 cggagcgtcg agctgatcga tttcgttcga gtgctcccaa tgcaggatgc gcccggcgcg
    23581 ttccagcatc gttcccatgc ccagcacacg cacgaaatcg ccccagccgt aacgaatcat
    23641 cacactggcg atctcgtgca ggcgcggcag atcgcgcacc acgctgaaag tttcccacaa
    23701 catccggcta ttatcccgtt tcgacaataa cgatattcta cccgagcagc gcgcaaaaca
    23761 tccggttttt gcacaggatt gaacaacaaa cctttgacgg gatatgaaat tttgtttaca
    23821 gtgcccccat tgcaatccgg gatacaccac caccatgacc cacgtcatga tgcggcgcct
    23881 gatggctggc ctgtctgccc tggctttttt gctgaccggc atccccggcc gggccgacga
    23941 ctcggtaccc gcagctgtcg atactgcagc tgcggcaccc gccacaaccg gcgttgccaa
    24001 tggcgagccc cctcgccctt cccgtgccga actggccatg tttgccatgg gcctgatcgg
    24061 tgccgtctat cgcccgggtg gcgacacccc gcaaaagggc atggactgca gcggtttcgt
    24121 gcgttacgtc taccatgaag tcgccggtct gaaattgccg cacaacgccg ccgccatgag
    24181 ccggatcagt accgtcatca gccgtgccga cctcaagccg ggcgacctgg tatttttcaa
    24241 gaccatgaaa aaagccttca cccacgtcgg catctacctc ggcgacaacc agttcatcca
    24301 cgcttccagc agccggaccg gcgttgtaat gatttccagc ctcgacgagt cttactgggc
    24361 gcaccgctac gatggcgcac gccgggtgct cagctcgatc ggtagcagcg ccgtgccccc
    24421 ggcggccgcg gcaaccatgc ccgcgcccga cccggccgtt ccggccgacc ccatcgcggc
    24481 tttcgcccgg ccctgaacgg ccacgccatc atcttctga
//
LOCUS       DAIANL010000076         5119 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1923146, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000076 DAIANL010000000
VERSION     DAIANL010000076.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923146"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1135 a         1746 c         1375 g          863 t
ORIGIN      
        1 gcaccggcaa accggcacgg cgcgcccgct cccagtaacg gatcgtccag tcgagcacgc
       61 gttcctcttc ccaaccaatg taggcatcct tgaacagcga caccagatcg taggtgatcg
      121 gcccatacac cgcgtcctga aaatcaagga tgccaggtcc gtcttccacc accatcaggt
      181 tgcgactgtg gtaatcgcgg tgcacataga ccggagactg cgtcagggcg ttgtcgagca
      241 gccgcgcgaa caccagatgc agacggcgca actgcccctc gtccggctcg acgccaagat
      301 ggcgcgccag ataccagtca ggaaacagct gcagttcgcg ttcgagcaac gcacggtcat
      361 acaccggcag catatcggca cggctggccg cctggatgcg caccagcgca tcgatggctg
      421 caagatacaa cgcatcggcg gtctcggcat tgagcgccga cagataggtg gtatcgccga
      481 aatcggtcag cagcaggaac ccctgggcga catcgcgttc cagcacgcgc ggtacacgca
      541 cgcccgcgcc ggcgaacagt tcggcaacac ggatgaatgg cagacaatct tcatgctgcg
      601 gcggcgcatc catcacgata taatgctcac cattggacag gtgaacgcga aaataacgac
      661 gaaagcttgc gtcagcaccg acaccggtca gctcagacac gagaccggga aagaccgtca
      721 tcaaccacga caggatattc ttttgacgct gcatgaatgt tccaggcgaa ataggccact
      781 tgcgtgcatt ctagcaccaa tcgaaccacc atccgtacaa tgccgactcg cctgaccctc
      841 cttgcggccg ccgtcgcgct gagcttcgct ccggtcgccg atgccgaccc gctgccgatg
      901 ctgcaaccgt ccggcacgct gacctttcct gacaagtcca cgcccaatcc gccgctgttc
      961 atcagtgccg atcagatgga cggcgtacag gacaaattcc tcaacgccag cggttccgtc
     1021 gagttgcacc gtggcgttgc ggtactgaca gcacacaaga tccattatac cctgagcgac
     1081 gatacggtca cggccaacgg cgacgtgtgc ctggtgcagc aagggctcac gctgctcggt
     1141 ccgagcctgc tgatgcacat gcaacagcag accggcacca tggaccgccc ggtgtatacc
     1201 tatgtcaacc cgatgccgat ggtgtccaag aacggctcgc ccggcgtgcg tcaggcacgc
     1261 ggcgatgcct ccgccatcca gttcgaaggc aacgaccact accggctcga ccatgccacc
     1321 tacaccacct gcccggcagg aaacaatgac tggcaactgc atgtcaagga cctgtcgctc
     1381 gacatggtgc agcaggtcgg cacggcacac aatgtggtga tcgatttcca cgacacaccg
     1441 atcctgttca cgccgtggat caacttcccg ctcaacgatg cgcgcaagag cgggttcctt
     1501 gcgccgacca tcggcaccac cggcaacagc ggcggtatca tcagcctgcc ctggtactgg
     1561 aatattgcgc ccaattacga cgccacgatc gacccgagca tcatcaccaa gcgcggcatg
     1621 caactcggcg gagagttccg ctacctcacg cccagcatgc acggcagcat cgcgggcgat
     1681 ttcctgcagg accacctgac aggcaccgac cgctgggacc tgttcgtcat gcacgacgaa
     1741 acattcaccc ccaacatcag cggtcatctg gtctaccagg cggtatccga caacaactac
     1801 ttccgcgacc tgtccaacca gctcaacctg acttcgctgg ccacgctgga ccaggaaggc
     1861 tcgatgacgt accacacgtc atggtggtcg gctaccgcgc tggtgcaaca atttcagacg
     1921 ctgcaggacc cgcaggcgcc catcgtgccg ccgtatgcac gcttgccgga cctgtcgtgg
     1981 caggccgaca agatcctgcc cagcggggtc gaagccaaca tgtacaccga tttgaccgaa
     2041 ttcgtgcatc cgaccctggt caatggcacc cgtttcgtcg cctatccgag catcagcatg
     2101 ccgctgacca caccatgggg ctaccttacg ccgaaattcg gcgtgaacta tacgcaatat
     2161 gcgctgggcc tcaacaacac ggcgccgcag tcgcaataca cgcgcacgct gccgatcacc
     2221 agcctggaca ccggcatgta ttttgaccgc aacgtgcaat atttcggcca cgcctatcaa
     2281 cagtcactgg aaccgcggtt gtactacctc tacattccgt accagaacca gaaccagttg
     2341 ccgaatttcg ataccgccat tctcgacccg gtcaactatg ccaccctgtt taccgaaaac
     2401 cgctatgtcg gctacgaccg gatcaataat gccaaccaga tcaccatggc catgacctcg
     2461 cgtatcaccg atgcgcagac cgggctggag cgtctgcaat tctcgctggg gcagcgcttc
     2521 tacttcactc cgcaaatggt cacgctgcct ggcgaaacgc ccatcaccag cacatcgagc
     2581 gatattctcg gcgacgtggg cgggcagatc aatgaaagct ggcgtgccga agcggccgtc
     2641 agttacaata cgagttctgg ccagaccgac gaccagagtc tggtcctcaa ttaccagccc
     2701 gagccgggaa aagtcatcaa cttcagctac ctgaccgtca gcggacaggt cgatcaggtc
     2761 gacatttcca cgcaatggcc catcagccgc cactggtatg gcctgatgcg ctatgattat
     2821 tcgctgctgg accagcaggt tgtcgaagga ctggccggca tcgaatacaa tggcgggtgc
     2881 tgggcgattc gcacggtgat gcaaaccatc gccacggcgg ccaataccac cagcacttcg
     2941 ttattcgtcc agctcgaact gaacgggctg gccaatttgg gctccaatcc agatgacgca
     3001 ctcaaactca gcataccggg atacatcaac agcaatgaaa tcataccgcg cacgcattaa
     3061 gcactccttg cacgcacata cgtgcgccga tatggatctg actggccgac ccagttcctg
     3121 gcttgccatc gcgctgctga tcctgctgcc gggccttgcc cttgcagatg ccccggcaac
     3181 cgcacaggtt gcgcctgccg cccagaccac acccagcgcc caggtggcgc cgaccggaga
     3241 ggccgccgca ccaccacccc taccgccagc tcctgcaacg gcagccgctc cgctgacccc
     3301 ggtcgatgac atcgttgccg tcgtcaacaa cgacgtcatc acccgccatg aactcgacga
     3361 acgctacgac cgcgccgtcc atcagctcga agcccgcaaa atcgccctgc cgccacgcgc
     3421 catcctggaa aagcaactgc tcgaccgcat ggtcagcgac cgtatccagc tgcaatttgc
     3481 cgcgcagacc ggcatccgcg tcgacgctcc gacgcttgac cgtgccatcg acaggatcgc
     3541 cgagcaaaac aagatgaacc tgggccagtt ccgtgacgcg ctggcacgtg acggcgttga
     3601 tttcaacaag ttccgcgacg acatccgcaa cgagatcatc ctcaccaaac tgcaccagcg
     3661 cgaagtcgac agcaaggtca ccgtgagcga agcggaagtc gacgcctacc tggcttcgca
     3721 ggctgcccag ggcgccaata acgagtacca tctggccgtc atcagcgttg agattcccga
     3781 caacgctgac agtgcccaaa tctccgcggc ccagcacaag gccgatgaag cactggccaa
     3841 gctgcgtgcc ggcgcagatt tcagccagat ttcgaccatg tattcgaacg cgccgaacgc
     3901 acttgaaggt ggtgatctcg gctggcggcc cgccgcgcag attccgcccg atttcctgtc
     3961 catcctgcaa gccctgaaac caggagaagt cagcaacgtg ctgcgcagca acaccgggtt
     4021 ccacatcttc aagctggtgg acaagcgcag caccgaccag ccgaccatca tcgacgagac
     4081 ccatgcccgc cacatcctca tcaagaccaa cgaactggtc agcgatgcgg atgcgaagaa
     4141 acggctggaa cagattcgca cgaagattct ggacggcagc gccagcttcg aggacatggc
     4201 gaagaaatat tccgacgact ccagtgcctc gaagggcggt gacctcggct ggttgtcgcc
     4261 gggtgaaacc gtgcccgaat tcgaacaggc catgaacgca ctcaagcccg gggaaatcag
     4321 cgaacccatc aaatcgccct tcggctatca cctgatccag gtactcgaac gccggcagaa
     4381 agacgtcacc gaccagaagc ggcgcctcgt tgcccgccag gccatccagc aacgcaagtc
     4441 tgaagaggcg caaaccgagt ggttgcgcga actgcgcgac cgcgcctacg tcgaataccg
     4501 cccactcaac ccatgaaatt tctggcactg acggcgggcg agccggcagg catcggcccc
     4561 gatctgtgca tccagtgggc cagccggccg cgccggcatg acgtggtcgt actggccgac
     4621 cgcgaagcgc tgcttgaacg cgctcgcctt atgcaagtcc cgttgcggat tgtcaactac
     4681 gagcctggca cgccggcagt tcggcagccg ggcgaactca ccgtgctgca tctggcacag
     4741 gcccggcctg cacaggccgg cattcccgat cctgccaacg cacaatacgt attgtcattg
     4801 ctcgatcgcg ccattgctgg ttgccgctcg ggagaatttt ccgcactggt gaccgctccg
     4861 ctgcacaaag gcatcatcaa cgatgccggc attgccttta ccggccacac cgagtacctt
     4921 gctgcggcaa cggccacgcc acatgtggtg atgatgctgg tcggcggcgg cctgcgcgtc
     4981 gcgcttgcca ccacccacct gccactgcgt gcggtggccg acgccatcac tgccgacagc
     5041 ctgacccgca cgctggacat cctgcaccag tcgctgcgca cccagttcga tattgcgcaa
     5101 ccgcacatcc tggtggccg
//
LOCUS       DAIANL010000077        36118 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2742441, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000077 DAIANL010000000
VERSION     DAIANL010000077.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 36118)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36118)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..36118
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2742441"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         7223 a        11767 c        10463 g         6665 t
ORIGIN      
        1 gtccagcgca ccggcacgca ccagcgattc gagcacgcgg cggttgatac agcggcgatc
       61 gacgcgcatc accagatcga acagatccgc gaatggccca cctgcctcgc gggccgccag
      121 catggcctcg atggccgact cgccagtccc cttgaccgcg cccagcccgt aacggatttc
      181 atgttcgttc tccggcacga aacggtaacc cgacacattg atgtccggag gcagcatgtc
      241 gatggcaaag gcacgcgcgt cttcatagaa cagccgcact ttatcggtgt cgtccatgtc
      301 agccgacagt gtggcggcca tgaatgctgc cggatgatgc gccttgagat agaccgtctg
      361 atatgccacc agcgcatagg ccgccgagtg cgacttgttg aaaccgtaac cggcgaattt
      421 ttccatcaga tcgaaaatgc ctgccgccag gtcacgatcg acgccttgac gctctgcgcc
      481 cgcaacgaag atatcgcgat gctccgccat ttcttcggct tttttcttgc ccatggcgcg
      541 ccgcagcaga tcggcgccgc cgagcgtgta cccgcccatg atctgcgcaa tctgcatgac
      601 ctgttcctgg tacacgatca ccccataggt tggcttgagc gtggcttcga gcatcgggtg
      661 catgtaatcc acgcgcgcgc gcccatgctt gcggttgatg aagtcatcga ccatgccaga
      721 ctcaagcggg ccggggcgga acagtgccac cagcgccacg atgtcctcga aacagtctgg
      781 ttgcagccgg cgaatcaggt ccttcatgcc gcgtgattca agctggaaca cggccgtagt
      841 gcgtgccgat ttgagcagcg tgtagctgac gcggtcatcg agcggcaacg attcgagtgc
      901 aaacggcgcc cgcccgtccg gcgacagacg cgccacgtaa cgcatcgccc agtcgaggat
      961 ggtcagcgtg cgcaggccaa gaaagtcgaa cttgaccagc ccggcctttt ccacgtcgtc
     1021 cttgtcgaac tgcgacacca ccgaatcgga accatccgcc acatacaccg ggcaaaaatc
     1081 gctgatcttg ccgggcgcga tcagcacacc acccgcgtgc atgcctacgt tgcgcgtcag
     1141 gtcttccagc cgctgcgcca gatcgaacag ttcgtgtacg tcctcttcat tatcgtaacg
     1201 ctcgcgcaac tgcggttcca tttccagcgc ccgagccagc gctaccggct tgacgccttc
     1261 gatgggaatc agtttcgaca gctggtcgca caggttgtag ggcagatcga gcacacgccc
     1321 cacatcgcga atgaccgagc gcgaacccag cgtgccgaag gtgacgatct gcgataccgc
     1381 ctcggcgccg tacttgtcac gtacgtaatg aatcacacgc tcgcgcccgt cctggcagaa
     1441 atcgacgtca aagtctggca tcgatacccg ctcgggattg agaaagcgct cgaacagcaa
     1501 atcgtagggc aacgggtcga gatcggtaat gcccagcaca taggccacca gcgaacccgc
     1561 acccgatccc cgtcccggac cgaccgggac atcatggtgc ttggcccagt tgatgaagtc
     1621 cgccacgatg agaaagtaac cggggaaacc catctgcacg atggtgccca gttcgaattc
     1681 gagccgttcg cgatattccg gctcacgtcg ggcacgctcc ccggcatcgg cgtagcgctg
     1741 cgccagcctt gccgccagcc cttcgctgga gcgggcgcgc aagtatgctt ccagacccat
     1801 gccatccggc gtggggaatt gtggcagctg gctttttccc aagggcaggt cgagcgagca
     1861 gcgacgggca atctcgacgc tgttggccag cgcagccggc aagtcggcaa aacgctgcat
     1921 catctccgca cggctggcga aatactgcga ctcgccatac acgcgtggcc gacggcggtc
     1981 acccagcagc gcgccttccg cgatgcacac gcgcgcttca tgcgccttga aatcgccagg
     2041 ctcgagaaac tgcaccggat gggttgccac caccggcaat tccagttcgg ccgcaagcgc
     2101 caggctgcgg cgcaagcagt cttccgatcc gttcagtccc gtgcgctgga tttcgagata
     2161 atagcgttgc ggaaaggccg ccgcccaacg ccgggcacgg tcgcgcgcgc cctcgcggtt
     2221 gccgcccagc agcaactgtc caacatcacc ccactgcgca ccggagagca cgataatgcc
     2281 atcggtaggc tccgcttcca gccattccag acgcaattcc ggacgcccac gcacccggtt
     2341 ttcgcgatag gcgcgcgaca gccattccga caggcggcgg taacccgcca gattctgcac
     2401 cagcagcaac acccgcgtcg gccggtcttc ggcaccgggc atgccaaccc acatgtcgca
     2461 acccacgatc ggcttgatgc ccaggccacg cgccgcctgg tagaacttga ccagcccgaa
     2521 cagattggcc aggtcggtaa gcgccagcgc gggcatgcca tcggcaaacg ctcgcgccac
     2581 cgcgtcatcg atgcgcacga taccatcgct gatggaatat tccgaatgca ggcgcaggtg
     2641 gatgaaaccc ggttcatcca tggcgtgctc cgggcaatcg gcggcaggca ctgctcgaat
     2701 tcattcctcc gcaaccgtat cctggccagg aaacagcact tcggtaaaac cgaaattgcg
     2761 caagtcgcga atgcgccgcg gatagagaat gccgtacaga tgatccactt cgtgctgaac
     2821 cacgcgcgca tggaacccgc tcaccgtacg gtcaagcggc tgccctgcgg cgtcgaatcc
     2881 ctgatagcgc aggcgacgat agcgcggcac cagcccgcgc atgcctggca gcgacaggca
     2941 gccctcccat gcctcctcct cctcttcacc cagcgcggtc agcaccggat tgatcagcac
     3001 ggtctccggc acagtctccg cctccggata gcgcggattg gcattgacgg caaaaatcac
     3061 cacctgcaac ccgacgccaa tctgcggtgc ggccagcccg gcgccatgct gcgccgccat
     3121 ggtatcgcgc atgtcagcca gcaacgctcg cagctccgga gtgtcgaagg cgctcaccgg
     3181 ctcggctcgc gccaacagcc gggcatcccc cattttcagc acttcgcgga cggccattac
     3241 agttctacct gggtacccag ttcaatcaca cgattggtcg gtatgtggaa aaatgccgtg
     3301 gcgctgctgc cattacgcgc catgctgacg aacaggcgtt cgcgccacag cgccatgccg
     3361 ggcatttcgg tggcaatcaa cgtctcacgg ctgaggaaga acgaagtttc catgaaatcg
     3421 attcctgcgc cattgataag cacctgctgc aatgcctgcg gcacgtcggg tgcttccttg
     3481 aaaccgaacg acagggtgat gcgatagaag ccatcgccca tgttctgcat ggacacgcgt
     3541 tcggacagat ctacccaggg catgttgcgt gtcttgaccg tgagaaagat caccgtttca
     3601 tgcaacacct tgttatgcaa caggttgtgc agcatggcgt gtggcacctt gtccaggttg
     3661 gtagtcagga acaccgccgt cccggccacg cgaacgggcg gatgcgcctt caggccggca
     3721 agaaacggat ccagttcgat ggactgggtc tgcaaccgcc gggccagcaa ctcgcgaccg
     3781 cgtttccatg tcgtcatcag ggtgaacatc aagccgccga tggcaagcgg aaaccagccg
     3841 ccctgtacga ttttcaccag gttggcgcca aaaaacgcca ggtcgatgct gaggaagaac
     3901 gccagccccg caagaatcag cccccggttc cagtgccaca tgcgtcgcgc aaccacgaac
     3961 accagcagcg tgtcgatcat gatggtcccg gtcacggcca gtccgtaggc ccagccgagg
     4021 ttgctcgatg atttgaagcc gagaatcagc gccaccacac cgatcatgat gctccagttc
     4081 atcgatggca cataaatctg gccgatttcg cgcgccgagg tatgcacgat gtgcgtacgc
     4141 ggcaggaacc ccagctgaat cgcctgctgc gtcatggaat atacgccgga aatcacggcc
     4201 tgcgacgcaa tgacggtggc gactgtggac agcagcacca tcgggtacaa cgcccatttg
     4261 ggcgacagcg catagaatgg attggcggcg gtggacgggt cgcccatcat cagcgccccc
     4321 tggccgaagt aattgagcag cagggccggc aataccagac tgaaccaggc gagccggatc
     4381 ggctgccggc cgaagtggcc catgtcagcg tacagcgcct cggcacctgt cacggtcagc
     4441 accaccgcac ccagcgacag aaaaccttcc agccggtgct gcatcatgaa ttccgcggcc
     4501 cagtacggat tgaccgcctg cagtaccgcc ggctcgtgca ggatctggtt gacccccaac
     4561 gccgcaatga ccgcaaacca gaccatcatg atcgggccga acaggatgcc taccgtggcg
     4621 gtgcccttgc gctgaatcat gaacaacgcc accagtacga tcagcgacag cggaatgaca
     4681 aagggcttga ggtccggcgt cgccacttcc aggccttcca tcgccgaaag cacggaaatc
     4741 gctggcgtga tgatgccgtc accgaaaaac agggccgcac cgaacagacc gagcagaatc
     4801 agggcattgc gcaccctgct gttgctgcgc agcaaacgtt gcaccagtgc catcagcgcc
     4861 atgatgccac cttcaccgtg gttgtccgcc cgcatgatga acaccacgaa cttcaccgac
     4921 accacgatca gcagcgacca gaacactagc gacagcacgc caagtacgtt gtcatggttg
     4981 actacaatgc catgctcgct gctgaaaacc gtctgcaagg cgtacagcgg gctggttccg
     5041 atatcgccgt acaccacccc cagcgcaccc gctgccaggg ccaatacccc gtgtttcttg
     5101 atcatttgcg gcgattcggc cgccttcatc attctagtat ccgggtccac gccgtcccac
     5161 ctccccgatt gcgcagcgca gataatacac catcgaccac aaagtcagaa ctgcagccag
     5221 ccagatcagc caggtaccga cgcgctgcag attgatgccg aaatgtacgc cactccacaa
     5281 cagcagcacg atggccgcca tctgcaccgt ggttttgatc ttgccctgca tcgacaccgc
     5341 gacgcggcca cgcgcgccaa tgctcgccat ccattcgcgc aatgccgaaa tggtgatctc
     5401 gcgaccgata atgatcagcg caaccagcgc atcgacccgg ccgagctgca ccagaacgat
     5461 cagcgcggcg gcgaccatca gcttgtcagc caccggatcg aggaaggcgc cgaacgccga
     5521 agtctggtcc agcacgcgcg ccagatagcc gtccagccag tccgtcaccg ccgccagcga
     5581 gaacatcagc gccgcaaaaa aatccgcctg ctgcgggccg atccagctgc gaggcagata
     5641 gaacacggca acaaccagcg ggatcaggag gatgcgcagc caggtcagca cattgggcag
     5701 attgagcggc atgtctatac gggaagaggt aacctgcgtg attctaaccc aagccatggc
     5761 cagacaacag cagcaacccg gcaatcagtg caggttacgg taaatctgtt ccgccagcgc
     5821 ctggccgatg ccatcgacgc gcgcaatctc ttcctgaccg gcattgcgca gttcgcgcag
     5881 gccgccgaag cgttcgagca atttctgccg ccgtttcggc cccaccccgg gaatgcgctc
     5941 cagcgccgat tcactgcgcg cccgcccgcg tttcgctcgg tgcccggtaa tggcaaagcg
     6001 gtgcgcttca tcgcggatct gctggatcag gtgcagcgcc gcatcttcgg gcggcagccg
     6061 ccgcacgctg ccatcggcgg tcaccagctg ttccatgccc gccttgcgtt ccgggccttt
     6121 ggccacgccg agcagcatca cgccgtgcgt gcccagttca ttcaatgcct cggccgccgc
     6181 tcgtacctga ccgataccgc cgtcgatcag caccagatcg gcggcctgct gcccgccgtc
     6241 gcgcacacgc gcgtaacgac gctccagtgc ctcgcgcatg gcggcataat cgtcaccggc
     6301 ggccgccttg cggatattga aacgacggta ttgcgacggc tgcatgtcac cctgatcgta
     6361 caccacacat gacgccacgg ttgcctcgcc cagggtatgg ctgatatcga aacattcgat
     6421 ccgctgcaat tcaggcatct gcaacgcctg ccgcaaggca tccagccgca actggcgggt
     6481 ggactgccgg gccagctgac gggaaatcgc cagccgggca ttgttgcgcc ccatgtcgag
     6541 ccatcggcgg cgctggccgg tgacccgtgc cgccaggctg acccgcctgc cggcgcgttc
     6601 ggcaagaaac tcgaccaggt ccggagcctg caccggctcg gaaaccacaa tctgcgccgg
     6661 tggagcagca tcgagatagt gctggctcag gaacgcctgc aatgcctctt gcgcagttac
     6721 atcctgcgca tggtgcggaa acaggctgcg gtcgcccaga tgccggccgc cgcgaatcat
     6781 ggccagattg acgcacacca gcccctgttc ggccactacc gataccacat cggcgtccag
     6841 gtcggcaccc tgctcgaccg actgacgctc gcgcactgcc gtcaggctgc ggatctggtc
     6901 gcggaatatg gccgccagct cgtactgctg cgcctgcgcc gcctgctgca tgcggtgtgt
     6961 cacatcgtcg aggatgcgat cggtcttgcc tgtgagaaac aggcgcgcat gaccgacatc
     7021 ggcagcataa tcggcggcgg cgatcgcgcc cacacacggc gcgctgcagc gcttgatctg
     7081 gtagagcaga cacgggcgcg agcggtgcgc gaacacactg tcttcacagg tgcgcaggcg
     7141 aaacactttc tgcaggatgt caatgctctc gcgcaccgca tgtgcattgg gaaacgggcc
     7201 gaaactgtgg tccgtgcgtc gcggcgcgcc acggaaatag gccagccgtg gccaggcatg
     7261 cccggtcagc agcaaccacg gatacgattt atcgtcgcga aacagtatgt tgtagcgtgg
     7321 cgacagcgcc ttgatcagat tgttttccag caacagcgct tcggattccg aagcggtgac
     7381 ggtgatttcc accgcagcca cccggcttag catcagacgg atgcgcggcg aatggtcgtt
     7441 cttgtgaaag tacgacgaca cgcgtttctt caggtctccc gccttgccta cgtatagcac
     7501 gtcgcgctgc gcgtcaagca tgcggtacac acccggcagg ttgggcaggc tggcgatcag
     7561 ctcggcggat tcgacctcat tcatgctgca tccgatcggc acggataccg gcaatgactg
     7621 cgtcgaacat gtcatcggtc agccggcgcg tctgggtgtt gtaacgactg caatgataac
     7681 tgtcgtacag acgcctgcca tcggccagtt catggcgcgc gccatgggca aacggtgcgc
     7741 tgcgcggcgg caaccccagg gccagcagca ccgcatcatg tgcgaccttg cccagcgcca
     7801 gcacactggc ctgtggcggc aggccggcca gctcgtcgcg caggtacgtg ttgcagcgac
     7861 gcatctccgc cgtttcaggc ttgttctggg gcggcaggca cttcaccgcg ttggtgatac
     7921 gacagccata cagttccagc ccatcgtccc gggcgctcga actgtcggcc gaggcaaaac
     7981 cgtgccggta cagcgtacgg tacagcagga ttccggcatg atcgccggta aacggtcgcc
     8041 cggtgcggtt ggcgccatgc atgcccggcg caaggccgac gatcagcagc caggcgcccg
     8101 gatcgccaaa cggtgccacc ggcgctgcgt ggtaatgcgg atgctgcgcg cgcacctgcg
     8161 ccagaaaccc ggccagacgc ggacaggcgg tacagtgagg atcgaacggt ggcgtcatga
     8221 atctccgctc agaccacagc tgttggcagg atcggtacgc cagaaatcac cgctgtcgtg
     8281 acccagcgcg gcaacggcat gctgtgcggc gacatactgc ggctgctcgc gcaacgtcac
     8341 ttcatctgcc ggatgcgccc ggccatgcag ccgcgcgcga cgcgcgatgc cgagcgccga
     8401 gggttgccat tcgagcgcgt gcagcacgcg gtcggctgcg gcgccgtcat tgcataccag
     8461 cagcatgtca caaccggcgt gcagcgcggc ccgggcacgc tgcacgatgt caccgccggc
     8521 ggctgccccg gccatggaca ggtcgtcgct gaaaattgcg ccgtcaaaac ccagctggcc
     8581 gcgcagcacc tgttgcagcc agaccggaga aaatccggcg ctgcgttcgt ccacggccgg
     8641 gtagcgcaca tgcgccggca tgatgccggg taatccgtcg gcaatgagcc gggcaaacgg
     8701 ccgcagatcc gcctgctgca actgttccag cggccggtcg tccagtggca ggtcggtatg
     8761 cgaatccgcc tcgacccagc catgaccggg aaaatgcttg gccaccgcgg ccatgcccgc
     8821 cacccgcatg ccgaccatca tggcatgcgc cagatcggcc accacgtcgg gatcgccgtg
     8881 cagggcacgg ctgccaatga ctgcagaccg gccataatcc agatccagca ccggcgcaaa
     8941 actcagatca accccgtgcg cacgcagttc ggcggcaagc acatagccga aatcctgcgc
     9001 cagccgcaag gcatgagccg gctgtgcgtc ccacagccgg cccagttcac gcgccgccgg
     9061 cagccgggtg aagccatcgc ggaaacgttg cacgcggccg ccctcgtgat cggtggcaat
     9121 cagcaaatgc ggcgtgcgta acgcacggat ctccgcagtc aaggtagcca gttgctgcgg
     9181 atcggcataa ttgcgggcaa acagtatcac tccgcccacc agcgggtgcg acaaccggcg
     9241 acggtcttcc gcgtccagtg caagccctgc cacatccagc atcaagggtc cgaggctcaa
     9301 tgtccaccct ccagcaccac gaatgccacc accatgtccc gttcatcgct gatggagaca
     9361 tgaccgacgc gcacgccccg catctgcaac cagtgttcga gcgccggcgc atatgccagc
     9421 gacgggcgac caagcgaatc atgcatcaca ctgagattga ccagactcac cggagcccgc
     9481 agcccggtgc ctgctgcctt ggcgaaggct tccttggctg caaaacgctt ggcgagatag
     9541 cgccccatgt ctggcagcgg caattcaggc cgctcacagg cggacagcac gtgtccggcc
     9601 agccggtccc catatcgctg ccacagacgt tccatgcgcg acacatgcac aatatcggta
     9661 ccaatgccaa aaatcacgca tgcacccgca cggccagacc acacatacgg acacgttccg
     9721 ccagccgttc gagctggctc tcgggtacgg cagacagatg cgcggccatc ggctgcattg
     9781 atggtcgcgc cagcccgtag agcagcacac cgttcacacc gctgtctgcc agcccgcata
     9841 cggcatccac ccaggcctgt tcctcgacgg catccggcgc cgcaccgtcc caggcgaacc
     9901 agcaggtatg aatccagacc gggcacagtc gcgcggccag ccgcaggtgt tccatctggc
     9961 gcgccggtgg cgtacgtgtg ccgttgacgc gacgcatccc ctcggcggtc gcgcgatcaa
    10021 acttgaacca caactgcccg cccagatccg ccaaccggcg caagccctgc tgcacgcgca
    10081 ccttgtcgag ctgactgcca ttgctgatga ccaccaggtc gaccttgccc agcagattga
    10141 aatggctcat cacccgcccg acgcactcga tcacctgtgg aaactcccgc gcactggtcg
    10201 gctcaccatc gccggacagg gcgatgtcac gcagttgtcg cgctccttcc ggaacatgct
    10261 tctgcatgaa atcgccgtgc agcaggtcat gcaggaattg ccccagttca ttctcgagca
    10321 gatcgagatc aaccggtggc gcacttccgc gctgcaggtc gggcacctga caatagctgc
    10381 agcgccagtt gcacgcatgg ttggtattca ggttgatgcc gaccgacacg ccgccagcac
    10441 ggcgcgacac gaccggatag acgtaggtca tgccgatgat gtcgctcgga tggcgctcga
    10501 cggaaagcgt aaatgtggtg ttcgtgctca agataagaat gcccaaaatc gaccagcggc
    10561 cagacgagcc aacccgatga tcagcatact gtgccgaccc ggcgaagtca aaatccgcct
    10621 tgggcggcag gcaggatgcg gtggcagtcc atggcaatca cgccatgcgc actgccggaa
    10681 aacagttgct cggcgcgcca tgcatcgcca gccatgccga gcagcacgaa cagcggtgcc
    10741 aggtgctctt ccgacgggtg gttgcgtcgc gcccacggcg cacgttcgcg ataggccaac
    10801 acgtcctgcg tgcgcccgtg cagcaacgcc tgggccagcc agtcggcaaa ttcgcgcgac
    10861 cagggctgct cgcgcgcctg tgctccctgc cccatcgctt catacaggtt gtgcgtcacc
    10921 gagcccgagc cgataaccag tacatcgcgg tcgagcaatg gctgcagcgc ctggccgatg
    10981 cggtaatggg catgtgcatc ggcaccacgc accaacgaga cctggcacac ggggatgtcg
    11041 gcagcgggaa acatcagtga caacggaatc cacgccccgt ggtccagacc acgctgtaca
    11101 tcggctaccg ccggaattcc tgctgcggcg agccatccca gacactgttg cgccagctcc
    11161 ggaactccgg gtgcgtcata gtgcatggca tacagcgcgc gcggaaagcc ggaaaaatca
    11221 tggatggtgt ccggacgcaa cgcggtgccg accgccacgc cgcccacatg ttcccagtgc
    11281 gccgaaatca cgaccacggc accgggtcgc tcagactgcc ggcccaaccc ctgcaaaaag
    11341 cgccgtgctg gcgaatcctc cagcgcaagc atcggcgagc cgtgcgagag caacaacatt
    11401 tgcgacgaca tgacgcctcc tctacattca gtgcatattg tagagcaaag tggacggtca
    11461 ggcggactcc gcggaagctg gtgcacgtgt gcccgtaccg ttcgccgcct gcgcaaaact
    11521 cacgcgcacg cccagaccgc cggcagcagc acgtgtcagg tcaagctgcg cctgatgacg
    11581 ttgcgcaatc gcacgcacaa tggcaagccc cagtccggtg cccggcttat tcgacccggg
    11641 aatgcgatag aagcggtcaa agacacgggc gtactcctct tcaggaatgc ccggaccgct
    11701 gtcagccacc tcgatccagc agatgtgacc atgcacaccg gtggacacat cgatccggcc
    11761 tccggccggg gtatagcgga tggcgttttc caccagattg tccagcaaca gcccgagcgc
    11821 ttcctcatcg ccatccacca cggccaccgg ctgctcggcc agcatgccca gatcgatgcc
    11881 acccgcctcg gccagcatca ggtgacgtgc cagcaccttg cgcgccagcc tgctcagatc
    11941 gacccgggtg atcacatcac gttcgccaac agactcgctg cgcgccagca tcagcagctg
    12001 ctcgaccagg tgggtggcac gcgcaacgcc ggccttgagt tctcctagtg attgcagtcg
    12061 ctcggcttcg tcgcgcgcac gttcgctgag ctggatctgc agcgtgagcg ccgccagcgg
    12121 cgtacgcagt tcgtgcgccg catcggcaac gaaagcgtgc tggctctcga aagcgcggcg
    12181 caaccgctca agcaggctgt tgatggcctg caccagcggc ccgagttctt ccggcatgtc
    12241 ctgtcgcgga catggttcga ggctgtcggg cttgcggtag ttgacccagc ccgccaactg
    12301 ctgcaacggc accagcccgc gggtgacgat ccaccaggtc atcaggccga tgaccggcaa
    12361 cgtgacaaga aacggcaggc tgacccgcag ggcggaattc ttggcgatcc ggtcgcgaat
    12421 ggccatcggc tgcgccacct catagatacg ctgcctgcga ctcaccgaat attcccgcca
    12481 cagttgcccc ccactgcgca cctgactata tccctcggta tgtggcgctg gcaggagggc
    12541 gtccggactg gaacggtata tcggctggcg ggtggctgcg tcaaagatct ctaccagaat
    12601 tgcagcattc tcttccggat cgatgtcctt ccgcatcgaa gcgaaatcgc catggaacag
    12661 cgagatggcc atttcacgca tctggtaatc gagcgattcg tcagcctcct cccgcgccac
    12721 acgccaggtg gtgtaatcgc ccaccagcaa cgccagggtc agcatgagca gcagcgaggt
    12781 cagcaggcgt cggcggatgg aattcatgaa ttgcccggca tcaaccagcc gacgccgcgc
    12841 aaattacgca cccaacgggg accaagcttg cgacgcaagg cgctgatgta gtactcgatc
    12901 acattgctgg tcacatcttc gtcccagccg tacatgcgcc gctcgagttc ggcacgcgag
    12961 aatggccggt cgggatgctc catcaggatg cgcaacaggg aaaattcgcg catcgtcagg
    13021 tggatgcgcc ggttgtcgag catgacgtca tgtgtggcgg gattcatgca caactgaccg
    13081 atctcgattg ccggttcgct gcgcccgcca cgccgcctgg cgagcgcgcg cagtcgggct
    13141 gccagttcgt cgagatcgaa cggcttgacg agatagtcat ccgcgccggc atccagcgcg
    13201 ccaacccgat cggccagcgc atcgcgcgca gtaatgatga gcactggcgc cggatgcccg
    13261 tgcttgcgca tctgccccag caattccatg ccatccttgc gcggcaaccc gagatcgagc
    13321 acgatgagat cgtaggcttc gtcaatcaac gccgaagccg cgcgcatacc gtcgcggatc
    13381 cagtcaacgg cataactctc gcgctgcagc cactgccgca ccgccgctcc gatcatcacg
    13441 tcgtcttcga ccaccaacac gcgcatcgcc gctccttcgc ccaggatatc cggcgccccg
    13501 gcccaccatt cgccgcagca gcaacacccc agaaaagctg aaggccgatt gccgcaatcc
    13561 acccggaaaa taccggatta tgcagttgca aacttatggt tacctgatca cagcgtgaat
    13621 tttttgtgac atgcaggcct gtctggcgca ggcatttcca cgctacaata gcgggtattg
    13681 ccctgcacca tcatttcacg gcgcaccagc cattttcagc gaaaccgacg ccatgcctgc
    13741 ccgatttgac tttgcccgca tccaggatct gcgcgaccgc ctcgacacac atccggtgta
    13801 ccatgcgctg catgacctcg atgacctgcg cgtgttcatg gcgcaccatg tgttttccgt
    13861 ctgggatttc atgtccatgg tgaagtacct gcaacagtgc atcgccccgg tccgcattcc
    13921 ctgggcgccg cacagccacc cgtctgtgcg acacttcatc aaccagctgg tgctcgaaga
    13981 agaatccgat accggcttgc cggacgccag tggccagccc acccatgcca gccatttcga
    14041 actctactgt ggcgccatga aagaaatcgg cgccgatccg gcggctgtcc gtcgcttcgt
    14101 cgcccgcgcc gcgcacgaag gcatcgaagc cgcgtttgca atggacgccg caccgccagc
    14161 cgcgcagcgg ttcatgcgca gcacgttcgg cttcatcgcc agccagcaac cgcacgtcgt
    14221 ggccgccgcc ttcgcgcttg gccgcgaaca catcattccc ggcatgttcc gcgccttttt
    14281 acagcgcatg gatattgacg aagccgacgc ccccaccttc catttctacc tcaaacgtca
    14341 cattcacctc gacgaagact ttcatggccc catctcgttg ctgttgctcg aagaactgtg
    14401 cgatggcgat gttgcccggc tgcgcgaagc agaacaagcc gcacgcaccg ccattgaggc
    14461 ccgcatcgat ttctgggatg gcatcctgca cgcgctgcgc aacggcacgc cgctcacccc
    14521 tgcgggccag gagcgccatg cgcacgccca cgtggacgac tccggcaagc acccggagac
    14581 agcgcagttc gggatcaggg ccacatcgcc tcaatcggac tgaaatcctt cggggcaaga
    14641 cacaagggaa gcggtgcctg attccacacg gtcacgacgt tggtctggca ggatgggttg
    14701 caaggcgcga ttcgaagtca atggctgaca cccttgacga gaagcacaac gcagcaagca
    14761 agctgtccgc cacaaccacg tcccctgcgg gttagggtga aatcgcgccc tcgctttacc
    14821 ggattccccg gcatcgcagt cccggctacg acctgcgtca tccggtgcgc gatcatcccg
    14881 atttcaccca caacgcgatc cgtgaagaat taatcaccgc gtcccaaggt acaattgcgt
    14941 ctgttgtcat ggtgcgggca gaacgctcgt ttaaacagat gagcctatca ggatgtaagc
    15001 atgcgctgac catcggccgg ctgatggtca gacgccttgt gatgttccat ggaaaatccg
    15061 gcttgacagg cgtgctgacg ctggttttta gtgccgtggc ccatgcacag gggtttgctc
    15121 cggttcctgt cgacgtgatg cagttcccac cgcagatgcg gggtacggtt gaccaggagt
    15181 tgatgcgtgg acaacttcgg catgaactgt tcaccatgac agcgcccgcg cgcaagcgct
    15241 ggcgcatcgc ctatcttttc cctcacctga acgatccgta ctggcttggt tgtgcatatg
    15301 gcgtcgtcag cgaagcgcgc cggctgcgcg tggctgtcga catcatccct gcggaaggtt
    15361 acgacgacct gatcggacag ctgcgcgaaa tgaacgaact tgtcgtcaca aaaaaatatg
    15421 atgcgattgt tttgtcgccc attagcctga ccggcgataa tctggtcatc aggcaggccc
    15481 ggaaatcggg cattccggta tttgaactgg ctaacgacag caccagcaaa tcattggtca
    15541 gcaaggtgac gacctcgatg gacggaatgg gcgagaatgc gacacactgg atgatggcgg
    15601 atgcccggcg gcgcggactg aaatcggtga acgtggcctt gttgcccgga ccgatggatg
    15661 caggctgggt gcagggggaa gtgaaaggca cattgaaggc gctccggaat gccccgattc
    15721 cggtgcatgt ggttacgatt cgttacggtg acagtgaacg catcattcag acacaactga
    15781 cggagcagat actggcgcaa tatggtcggc atctggatta cattctgggc tgtacagggt
    15841 gtgcgccagc tgccgtattg ccggttcgtg ccgttcgcct gaacagacgc atccggatag
    15901 tggcctatga tttgacggat gaaattgcgg ccatgttgcg tcggggcgaa atcagggctg
    15961 caggcgatac caaaggtgtc agtcaggctc gggtggttac ggatctggcg gtggcctatc
    16021 tggaaaatcc gcacaaaccg gtgccggata ccgttctggt aggcttggga atggttgaca
    16081 caggcaatct gcagcgttat ccgatagaaa catccatcgc gccgcaaggt tacacgccgg
    16141 tactttcctg ggcaccgggc agtggagtgc caggcccgaa atcgagagcc ggactccatc
    16201 catgaaatgg tcgctgaaaa acaagatcct gttcggcgtc ttgctgtttg ccgtgctgat
    16261 ggcttccgca tacatgggtg cggcatctgt cgtgatgcgt cagcaatatc tggatgaatc
    16321 gcatcgtatg ctgaaaaatg ctgcgggcat cgtagaagcg gcattgaacc tccgacgcga
    16381 acgggtacag tccgcggctc gcgatctggc gagccagaaa aatctgggcg cggatctctg
    16441 gtacctgacg cgctatgggc aggagaatct ggatcaggaa acgttgtcga gcacctatcg
    16501 gcaattggcg caagatgttc tgcagattgc ccgcagaaaa aacattcccc gactggccat
    16561 ttatgacatg gcaggtcatc tgctggtatt tgcccggaaa gatgatgccc actggcagac
    16621 cggattcgta cagacagttt caaccggcgg gatacacctg gcaaatatca gcccgacggg
    16681 cgagttacgc tggcaaccca tgtcggcaac ccgttcgctg gcgtcattgc ggttcgatga
    16741 agcgattccc cggacagaac gggtgtttta tgcccgggtg gatggtcatc tggtgatccg
    16801 cgccgaaata ccggtcatgg gtgaaatgtt tgagccgggc aacaacggac cggtcaggga
    16861 gccgatgggg tttgtggatg cgattctgcc gtttgattcg acatatttgc agcagattgc
    16921 acatctgacg ggcacgcatg tcaatctgtt cctgggcaat gtactggaca tgggcgacat
    16981 agcgagctat cgcagcgtaa accggatgag cgactacacg acgttaccgt cagggaatct
    17041 gctgttcaat acagtgaata ttcaaggcaa cggctattat caatgcctgt ttcccctggt
    17101 gggcgatcat ggtgtaaccg gttccgtggc gttattgcaa tcgatggaag tgatcaacaa
    17161 aaacatctgg cagatgatcg gcatcttgtg gtggatcgcc ttgggcagtg ttttactgat
    17221 catacctctg gcctggtatt ttgcagtgtc attgaccagc ccgatcacat tgctgatcgg
    17281 aatgtttcgc aagatggcac gtggagacag gcggcgctgg tccgcacgcg aacggctgtt
    17341 gatgcatggc gcacagcggc ggcgggatga gctgggtgag ttggccctgg gcttcatggc
    17401 gatgaatgat gccattgcac aaaagatcga tgaaatccgt gacatgaatg catcgctgga
    17461 acagaaggtc gccgacagaa cggcagcgct gtcggcgaag gaagcggaga ctcgtaccct
    17521 gattgaaaat tcaccggata ccatagtgcg ttacgatcgt gaatgtcggc ggatttatgt
    17581 gaaccctgcc ttatccgatg cgatcgaagg cggcagcgcg gccgtgttgc acaaaacacc
    17641 gatcgagacg ctgggaacag aggagggcca ggcctatcag gataaaatcc gccaggtgtt
    17701 tgcgacggga ttgcccgtag agcacgaagt cacatggcgc aataaacagg accggaccgt
    17761 gcacagccat gtccgcctga caccggaatt tgatgccagc ggcaacgtca gctcggcatt
    17821 gggtatcggc cgcgacatga cggaccggat ggctttcgaa caaaccatct ggcggcaagc
    17881 caattacgat accctgacgc acctgcccaa ccggcagatg tttcaggata cgctgtatca
    17941 ggaaacgcaa tccatgctcc ggcatggaca ttcgctggcg ctgttgctga tcgatctgga
    18001 tcggttcaaa gaagtgaatg atgccctggg acacgataaa ggggattcgt tgctgattca
    18061 ggctgcgcat cgcatccgtt cctgtgtgcg ggaaaacgac actgtggcac ggctgggcgg
    18121 cgatgaattt acggtcatca tcggtggttt cgacgatgtg acaaccgttg agcgtattgc
    18181 acgtgacatc atagaccgat tgagcctgcc cttcgatctg gcaggcgaac aggcttatat
    18241 ttcggccagc atcggaatca gccggtatcc acaggatgcc actgaactgg atacgttgtt
    18301 caagcatgcc gatcaggcaa tgtacgccgc caaaaacaat ggacgcaacc agttttatca
    18361 cttcactccg gatttgcagc aggcagctga catgcgcctg cgcatgacca atgatttgcg
    18421 tcacgccctg gctgatggac aactggtcat tcattatcag cccatcatcg atctgagcag
    18481 cggaaaactc tacaagatcg aggcattatt gcgctggcag cacccggaac ggggcaccat
    18541 agacccgacc agtttcattg cgctggccga agaaaccggg ttgatcgtct ccattggtga
    18601 ctgggtgttt ggcgaagtga tccagcagac gaaatcctgg cagaaaaaat gcgtcaaccc
    18661 ggtgcgcatc agcgtcaaca agtcgcccgt gcagattcat cagcgggatc agcctgtttc
    18721 atggccagag gttgtgcgca cggctggtct ggatgccaga caggtgacca tcgagattac
    18781 cgagggcgta ttgctcgatg ccaatgatga tgtcagagca cgcttgaccg aattgcggca
    18841 ggcaggcatg caactggccc tggacgattt tggcacagga tattcttctt tgtcttacct
    18901 gaaaaaattc gatatcgatt tcctgaaaat cgaccggtca tatatccagc atctggagaa
    18961 tgatcccgac cagcgagtcc tgtgcgaagc aatcatcgtc atggcgcata ccctggggtt
    19021 gcaggtgatc gctgaaggcg tggaaactgc ggcgcaacaa caactgctgc tcgccgcagg
    19081 ctgtgattat gcacaggggt ttctgtatga ccggccattg cccgcagaca tgttcgagca
    19141 acgatggctg attgccggaa aacctgcctg agatcgttac tcacgcgttt acccaacgcc
    19201 gcagcgcacg ccacttttcc tgaggaaacc gggactggat gccgtctggc gatgcacgct
    19261 ctattggtct tacgcacatc gagatgtcaa gaacaaagtc cgccagtaga agtgaaaacc
    19321 cccgcgagac gtggcatctg gcgaggccat cggaatgcaa acagggcatc tttgcaaggt
    19381 taaatggtgg cggatgggca ccggttcaat tccgtccccg ttgcttggat agcaatgcag
    19441 cggcaatggg cgatagtgag tcgtcacgtc ccaaactcag gctggtgttt ccccgacaga
    19501 cagaaagtcc ccgtggtcgt catcgctcaa actgatgtat tccaaccgcc actgatccac
    19561 gcgccggagg tcggccagcg tgatggaggt gtcggcgacc gcctcgcagg catcgagcgc
    19621 gatttctgtc gcagcgtccg gcttgccata agcttgcccg aaaagctcac gcaactggct
    19681 ggcgacttct tccgcagact ctcgcgcctg cgtaccgccc tcggtggcgt cgtacaccac
    19741 ggatatcgac agcgcgtagg gttcccccgc cgggacttcc gccgacctct gctcgccaag
    19801 atcgaagaac agacccacca gatgtttggc tttgggctca agaattttcc cgatctgccg
    19861 ctcgaccgaa ttcttcctgc tcacaatctg ccgcaaccga ttctcgaagg cgtttgggaa
    19921 cgccggtcgg ccataacgcg ctgcgagcca ctgcttcagc gcacgtttcc catcaatggg
    19981 cagcgatgcg tggtcatctc tggctgcgtg tttctcgaac tcacccttgg gcacgatgcg
    20041 ccgctctgcg tgccgaagct cgacgacgac ggactgcccg cccgcgacct cataacctag
    20101 atggagcttg cgcgggttct tcgcgtagga gaactgcggg tttggcgcgc tgacgacgtc
    20161 ggctacgatg acctccaccg aaatttcgcc ttcgtgagga atatcacagt cgtgcgtgat
    20221 gacgacgacg cggtgcgctt cgcagaccgc gccgttcagc gctgtcaact tgaccgcagt
    20281 ttcctgagta agcaggtcac cttgtcgcca atcggtatcg cgcgtcagca tccatcgtcc
    20341 tctgctctat cactcggagg aaccggggat ggaaagctcc gcgcgccact cgtccgacgg
    20401 cgcggccttc gacttcgcca agccggaacg gctatatgcc gcatccatgg cctgcgcttc
    20461 ggcaatcagc gtctgggtgt gttcgggcac aagctgccca gcggcaacca agtccagcag
    20521 cgagtgacca tcgaatgcct tcatcttcaa cagggatgat gcgcgtgtca caccggcctt
    20581 ctggaaggcg tcggcggcaa ggctgaggga gcggatgcgt tccaacttgt catcttccgg
    20641 cgtggattcg ccgccgatcc acttataaat ggcctgccgc gaaacaccga acgccgtcgc
    20701 caagtctgcg atggcggggt tcagcacctg ccggatattc gcaagatgct cggcggcgga
    20761 acggatgtcc agacgcgtag catccacatc aaccgtttgg acgtcgaaat aaggaacgcg
    20821 cgtgtccagc atcttgcccc actcattggc atgggagagc gcgaacacac tacctgtgcc
    20881 accaagggcg caggaaatca cgacgacggc aacgccgatg ggctgctgaa tcggcatgga
    20941 gcgacgagca ggcaaggttg tggggtacat aattgcctcg attcgattct ggttgcgtca
    21001 ggcccacgcg ctgcgcgcgt gatccgttgt cgtcgcaccg aacaccgatt tgatcgtgcc
    21061 gtgcagcgaa agcagttgct cgttcagcct gtccatctcc accggtatcc ggccttggac
    21121 gtaatgatcc gtgtcgatga tcgcgtgttc gcgtgcttcc ttgatatcga aactgggcgc
    21181 aatcaccaat ccagtaggaa gcatatccgg cgggaagccc aaaggggcga ccgtgcggta
    21241 caccctgacg accaacgttc ccttggccac gagcggaaaa gtatccgtgg cgaatacagt
    21301 ttcggtcatt gtgtgctgac gggttgcatt gaaatcaatg ccatgcaatc cgcccaccag
    21361 atactgctcc gtggtttctc cggcttgtgg caagacggca tccagataac gaagccccag
    21421 ccgactgaca tggtcgagtg aagcggcttc gtgcaccgcc gtcaggccgc gcaacaattc
    21481 aggaatgaat tcgttgtgtg tctggtagtg ggtcgtgtgg taggtaatcg acgacggccc
    21541 aaggatgaag ccggccgtcc ggtcactgcg cgtcatcaac cacgacaccg cctgctgaat
    21601 ctgcggctcc tgcagctgtg cttgccccgg ccctggccct ggcacgatca attgcatgaa
    21661 ctgttgcgac tcgaacagcg gatacccctc gcgccgcagc cgatcctgaa tctgatcgac
    21721 gtacttgacc attgcggcaa cgggattgaa atgcgcctgc gccaacgcgt agtagacagg
    21781 cgcattcgac atcttctcgc tcacaggcag ctccggttct cgcgtggttg acagttttgt
    21841 tgtcagttta caccacagtt gacagtggca ccacttgtca gcagcgatcg cctcacgatg
    21901 gacgcctgat ctgccactga gcacggcaat catatcgatt gacaataccc attgagcggt
    21961 agtgatgcgc tcctctgtgc cgggtgcgct acctgcttta ttggccatca tccgccttgt
    22021 ttttcttctc ggtgcgacgt gcgggcgcag gtgccgaatt tccggaagcg agggtgtgaa
    22081 gatgttgtac tgactgctta tgctgtgctt acggacctgt ttttcatgca cagcaaaaag
    22141 ccgccggctg tcggagcctt ttcgctacat ccacaaacat ttttcaaatg ccaagcaatt
    22201 atgcaagcat ttgtttttat tggcggaagc ggtgagattc gaactcacga tgggtttccc
    22261 cacgccggtt ttcaagaccg gtgcattcaa ccactctgcc acgcttcctg tgtatgacct
    22321 gcaacagatt tcaaggccgg tgcaaccagc caccccgacc actcccagcg cacggcctgc
    22381 agtgcgctcc ggtacccgcc atgttccggc atccggcttc ctgtggcacg cgacgcggac
    22441 acccgtacgg aaccaaccgt gggattttag cctaaatccc cggtctgctc cagttccacc
    22501 cgagttcggc tccggctgac cttctgcgtc aacttatggc cgggcacggc aatctgcctg
    22561 gacgtttttt gcgcgtcaga ataattgttt tttataaaca tggcattata tctagtacgt
    22621 tatttgctgc atgacgagca attatcccgt tcgagtacac ccatgatcaa aaacctgttc
    22681 gagaagctgg gcaagaaggg agtttcgcgg gcggattcac cgctgaacaa cctcgaagcc
    22741 gcgcaccggt gggtcgcgca attgcatggc ttgccgcaac tggaggcgca tcgccgcgcc
    22801 attgcgctgc tggaagaaca caaccagtcg aacaacctgg ccttcgatgc gcagcgcatc
    22861 gacatcctcg gactgatcga acacgcagga tcagaactgc agtatgggct gatcaagcat
    22921 ttcttcgagc atctggccga cccgcagcac gaacaggcgg tctactggca cgaaatcgtc
    22981 gccttttatc agctgctggc ggaaacctac ctcaatcttg ccctgcgcga cgccaggcag
    23041 tcacgcccgc cggcgtcctt gccgaccatg gtgctgcgcg cgctgcatta ccaggggaaa
    23101 tcgatgcaat ggcgctatct gcgctttgag ttgccggagc cgaaacactg ggctacgttg
    23161 caccgcctgt accagatcgc atgtcagcat gagtttgctc acaaaaacat ggtgctcaag
    23221 ggcagcgcat atgcgacctg cgagggggtc tacgcccggg tgctgatgct gcatctgatg
    23281 cggccggccg gattgcctcc caacgaaatc gaactgacgg cctactggtc gtggaaatgg
    23341 cgcgatacga tctcgttgtc gagcgaatac aacccggcgc tgcatacgca tttcgtctcg
    23401 ctacacagtg acgcaccccc gcaatcgtgt tcagcgcacc accggggggc agggccggcc
    23461 cacgtgtact ggtcagtcaa catggcgctg gaacaggcgc accagcaact cagtggcaac
    23521 ggcgatacac aacccatgca ggtcaagctg tacggcgtgc cttatactgc ggacaagtcc
    23581 atcgttatgc agcacatcct gtcgcggctg gcaacgcgag ccgcgctgcg accggatttt
    23641 tcccgcctga tcggcgaaat cgaaatgcaa tgctgcgact cgaccattgt caagcaactg
    23701 gtcgatccgc ccagtgccgt gcggatcaat gcccgatcaa gcagcgcggg gcatcccgaa
    23761 gaaccgtact tccggttgaa catcgtcgaa gacgtgagcg gctgccgcct gaaatccaat
    23821 cagctgatac tgctggaaac gacgggtgga caccccgccg aattggccac catccgctgg
    23881 atcgagcaaa aaggtgcccg actggtgact ctgggtgtcg aacggctggg gtcgcacccc
    23941 aaactggtga ccttcatcga aatcgccgac ccggcggagc cgtgcgcctt caatccggac
    24001 agcaccaccg gggccatcgg cattgcgttg tccgatcccg gagcgctcat cgtgttcggc
    24061 ctgccgtcgc aacgccacct ggatctgcgc gaagatgaat tcgtctaccg cgtgcgcatg
    24121 cgcggcatca tggaacaaaa tccgcagtgg atgctgttgc caatagcgcg gctcacccgc
    24181 acccggctca tggaaaagct tgccgcctga gcgtatcccg actggcccgg ctcagggaaa
    24241 ggtgaggcca gacgtcagcc attgctgttc ctgagccagg tcacggtgtg gcacgtcagc
    24301 gatatgaaat gagaacgcac ccttgcgacg gtcctcgaaa tattgacgca aggaatactc
    24361 caccgtgagg aacgcaattt cgtcccaggg aatctcgtct tcggcaaaca gacgcacttc
    24421 gaggctttct tcaccggcgc tgtattcggg gcgaagcaga cgcgcgcgga acatcagata
    24481 cacctgactg atgtggggca gattgcacag gctgaacaac tggacgatct ccacttcagc
    24541 tccggcctct tcctgggtct cgcggatggc accctgcagc gtggtttctc cattttccat
    24601 gaatccggcg ggcaacgtcc acttgccata ttgaggttcg atcgcgcgac ggcacaatag
    24661 caccttgtct tcccattcgg gcagacagcc caccaccatt ttcggatttt cgtaaaacac
    24721 cgtctgacag gtcggacaca cgtcacgcag acggttgtcg ccttccggga tgctccgcgc
    24781 cacaaccgtg ccgcactcgc tacaaaattt catcatgact ccaatgccat gccgacactc
    24841 atcatgcgtc ggggaaacat gatgccacgc tcatcccgtt cagccaagcc tgcgaactgc
    24901 gtcccatcat aaatgcggta catgcctgcg gcgaccgtgt gcagctgggg atgtcggccg
    24961 tgcagcagat cctgagcgat atcggctgcg acatccagcc tgggcagatg cgccaccagc
    25021 gcatcgacag gcaacaggat gtcatcgcgc accgtttcgt ccacctgttc gagccagttg
    25081 atcccatgtg cctgttgcag ttcgaatccg ccgctgctca ggcgcaccag ccgcgtcaga
    25141 tgcgccccgc aacccaattg ctcaccgata tcttctgcca gcgtgcgaat gtaggtgccg
    25201 gcactgcagc gcacctcgat gctcatgcga ggcgcatcaa acgcgatcag gtcgagcgca
    25261 tgaatttgca ccgttcgcgc ttcgcgtacc agttcaatgc cggcgcgcgc gtattcgtac
    25321 atcgggcgcc cgtcgacctt gagcgcggag tacatgggcg gtacctggga gatgtcgccc
    25381 cgaaacgctg ccagggcgcg ttccacggca gcgcggtcga cgtcaaccgg tcgggtctga
    25441 acggtttcgc cctctaggtc gccggtcgtg gtacgcacac cgagcagcac atcggcacga
    25501 taggtcttgt cggcatgcaa ctgccaggac gcaaacttgc tggcttcacc cagcgccacc
    25561 ggcagcagcc cttcggcaaa aggatcgaga gttccggtat ggccggcctt ctcggcacga
    25621 aacagtcgcc gcacctgctg caacgcggca tttgaagtca gaccggaggg cttgtccagc
    25681 aggaatacgc catgcacggc acgtcgcttc ggccgagtat tgcagtttcc acccatgcct
    25741 tgcgccggct cattccggcg tgtcgccgga cggctctggg gacgctacgg cttcatcgat
    25801 caggtgcgac agataggcac cgcgtgcaac ggatgtgtcg tagatgaaat tgagttgcgg
    25861 caccagacgc agacgcatgc gtggccccag ctgggagcga atgaagccgg cagcatgatt
    25921 gagcgcctgc atggcctgca gtgcccggtc ctgcggcccc atcacggtga aatatacctt
    25981 ggcgctttcc agatcacgcg tgaccaccac ctcggtgatg gtcaccatgc cgacgcgtgg
    26041 atcatgcacg ccggagcgga tgatgtcggc cagttcgcgc tgcagctgct ctgcgacccg
    26101 actggaacgc tgaaagtccc tggccatgat cagagcgacc gagccacctg gacgatttcg
    26161 tagacttcca gtttgtcgcc cacctccaga tcgtggaagc ctttgagcga aatgccacaa
    26221 tcgtagcctt cgcgcacttc cttgacatcg tccttgaaac gcttgaggga atccagctcg
    26281 ccatcgtgca ccaccccccc gttgcgcaga acgcgcacac gctcgttgcg tttgacgacg
    26341 ccatcgagca catggcagcc ggctaccgtg ccgatcttgg agatgacgta cacttcgcgc
    26401 acctcgacca tgccggtcac cacttccttg agttcgggtg ccagcatgcc ggacaatgcc
    26461 gcttttacca ggtccaccac ctcatagatg atgttgtagt agcgaatgtc cacgccgagc
    26521 gattcggcca gcttgcgtgc ctgcacatcg gcacggatat tgaatccgat aatcacggcc
    26581 ttggaagcca ttgccaggtt gacgtccgac tcggacaccg cgcctacggc gctgtgcagc
    26641 acatccacct tgacttcgtc ggtagaaatc ttctgcagcg ctgtcgtcag cgcttcgacc
    26701 gaaccctgta cgtcggcctt gatgatgagt ggcagccgtt tggcctcgcc ctcgcccatc
    26761 tgatccatga tgctttcaag cttggccgcc tgacgcttgg ccagctgcac atcgcggaac
    26821 ttgccctgac ggaacagggc gatttcacgc gccttgcgct cgtcatgcag caccatggca
    26881 tcctcgccag cacgcggcac atccgacagc ccctgaatct cgaccggaat cgacggaccg
    26941 gcttcatgca ctgccttgcc atcctcgtcg agcatggctc gcacgcggcc gtacaccgcg
    27001 ccgaccagca gcacgtctcc acgatgcagc gtgccggatt gcaccagcag ggtagccacc
    27061 gggccgcggc ccttgtccag ccgggcttcg atgatcaccc cgcgcgccgg cgtcgcgtgc
    27121 ggagccttaa gttcgagcac ttcagcctgc agcaggatgg cttcgatcaa cccgtcgata
    27181 ttgatgttct tcttggccga cacctcgacg aactgggtat cgccacccca gtcttccggc
    27241 acgacgccct gcgccacgag ttcctggcga atgcgctcgg ggttggcctc gggcttgtca
    27301 atcttgttca cggccaccac cagcggcacc tccgccgcac gggcatggtg aattgcttcg
    27361 atggtctgcg gcatgacacc gtcgtcggca gccacgacca gcaccaccag gtcggtgacc
    27421 ttggcgccac gggcacgcat ggcggtaaac gcctcgtggc caggggtatc gaggaacgtc
    27481 accatgccgc ggtcggtttc cacatggtag gcgccgatat gctgggtgat gccgcccgcc
    27541 tcgccgctgg ccacccgcgc ccggcggatg gcatccagca gcgacgtctt gccgtggtcg
    27601 acgtgaccca tgacggtcac caccggcgca cgcggcgaca gttcgacctc atgctgttca
    27661 gtatcttcga ggaaggcttc cggcgtgtcc agcggcgccg gcttggcgac gtggcccatt
    27721 tcttccacga cgatcatcgc ggtttcctgg tcgagcacct gattgatggt caccatggtg
    27781 cccagcttca tcagggtctt gatgacctcg gtagccttga cggacatttt gtgcgccagt
    27841 tcggcaacag tgatggtttc cggcagcagc acttcctgca cgatgggttc ggtcggcatg
    27901 ctgaacgagc tgccgctatc ctgatcaccc ttgtggtgac gaccgccgcc cttgcggttg
    27961 cgccaggcat ccgcaccgcc ggcatcgccg cgcgatttga tgccaccgcg cttgcggccc
    28021 tgggcatcgc tccacgcgcc ttctttcgat tttcccggct cacgcccctt gcgctcggcg
    28081 ggtttgtctg tacgcgctgc ctcaccgggc ttggcggcgg gcttgtgcag cgtgccttcg
    28141 gtcgtggccg gcttctgcgg tgctgccggc gccaccggtg cggccgcttt ggcggcagcc
    28201 tgttcctggg ccagcttgcg ccgctgttcg cgttcctgct tgaggcgcag ttcttcggcc
    28261 tgacgctcgt gcagcgccga atgacgacgc gcctcttcgg cgcgccgtgc aatttcctgt
    28321 tcgtccagca ccggccggat cacgcgccgg cgcagcgttg gtgtttcggc agccgccggt
    28381 gcttgtgctg ctgccacttc ttcggcgggc gtttcagccg gttctggtgt gacggcgaca
    28441 acctcctcga ccggttcggg ttcgaccaca ggttcgggag cgacgggctc aaccaccggc
    28501 tcgatcactt cttccgcgac gatctgctgc ggctgttctt cttcgaccgg caccagatct
    28561 tcagcctcaa ccaccggttc ctgtccggcc accggaagtt cacgcttgac cagcacacgc
    28621 ttcttgcgaa cttcaacctg gatggtacgt gccttacctg tcgtgctgtc ggcgcgacgg
    28681 atctcggagg tttccctgcg agtcagcgtg atcttgcctt tctgctcgcg cgctccgtgc
    28741 ttacggctca ggtaatcgag caagcgcgcc ttgtcctgct ccgtaatgtc gtcggcagcg
    28801 gcggatttgt ccaccccggc cgcgcgcaac tgctccagca gcaatcctgt ggagagtttc
    28861 aattcctggg caaactgttc tacattcatg acaaggttac caacaccttt tcagccgttt
    28921 tcaggcaaac cacggcgcac gcgccgccat aatcaactgg gatgcccgat cggcgtccac
    28981 gcccgtcatc tccaccagct catccacacc cagctccgcc agggcttccg agtccttgat
    29041 gccatgctcg gcaagtttgt gcatgagcgc gtcatccatt ccttccagcg ctgccagatc
    29101 ctccgcgccg tgttcgacct tttcttcgtt gacgatggca tcggtcagca gcgcatcacg
    29161 tgcccgggca cgcagttcat tgaccagatc ttcgtcaaac ccttcaatac cgagcatctc
    29221 gccaatgggc acgtaggcca cttcctcgat gctgccaaat ccttcctgcg ccaggatatc
    29281 agccacttcc tcgtcgacgt cgagcttgct cacgaacagc tcgcgcaggg caagcgcctc
    29341 gccctcgctc ctgcgccggg cttcctcgac ggtcatgatg ttgagttccc atcccgtcag
    29401 ttcggacgca agccgcacgt tctgcccgct gcggccgatg gcctgggcaa gctggtcttc
    29461 ttccagcacc acgtccatgc tgtgcttgtc ctcatccacg acgatactgc tcacctcggc
    29521 cggcgccagc gcattgatga cgaattgcgc cgcttcgggc gaccagagaa tgatgtcgac
    29581 ccgctcgcca gccagctcgg ccgtgaccgc ctgcacacgc gaaccgcgca tgccgacaca
    29641 ggttccgata ggatccagac gctggtcgtt cgattttact gcaattttgg cacgaacgcc
    29701 ggggtcgcgc gctgcgccct tgatctccag cagcccttcc tcgatttcag gcacttccag
    29761 ctcaaacagc ttggcgatga attcgggcgc gatgcgcgac aggatcagct gcgggccacg
    29821 accggaacgg tcgacacgca gcagataggc gcgcacgcgg tcgccgacac gcaggttttc
    29881 cttggggatc atctggtcac gcggcagcag cgcttcgatg cggccagact cgacgatcgc
    29941 gttgccgcgc tccatgcgct tgatggtccc ggtgaccagg aactccttgc ggtcgaggaa
    30001 atcattgagg atctgctcgc gctcggcgtc acggatacgc tgcagaatga cctgcttggc
    30061 ggcctgagcg ccgatacggc cgaactctac cgcttccagc ggttcacgca cctcgtcgcc
    30121 gatctgcatg ccggggtggg acacctgtgc gtccgtccag gcgatctggc tgtcaggaaa
    30181 ttcgtgctcg tcgtcgggca cgatctgcca gcaacgccag gctttgtatt cgccggtctc
    30241 gcgatcgatt tccacgcgaa tgtcggcatt gtcgtgaaaa cgtttcttgg tcgcggacgc
    30301 caacgcctgt tccagcgcac cgaacacgat atcacggttg acgttctttt ctctcgccag
    30361 cgcatcgacc agcaataaaa tttcgcggct catccccgaa aacctccaga tgcatcatcg
    30421 atcacagttc gggcaccagc cgcgcctgat cgatattgtc catttcaaat gccatttccc
    30481 ggcccgccac gtccagcacg acttcggccg ggtccgcatg cagcagcctg cccgcaaagc
    30541 ggcgctgacc cgccagcgcc acgcgcagtc gcaactgcac ttcgcgaccg gcataacgct
    30601 gataatctgc cagtttcctg aggggccggt ccattcctgg ggaagagact tccagccgct
    30661 cgtaatccat attctcggct gcaagccagt gactgaggtg gttgctcacc agcacgcaat
    30721 cgtcgagcgt cacgccctcc ggcttgtcga tgaacacccg catcaaaccg cttttggccg
    30781 agaacgaata atccgtcaat tcgtaaccga gaccggcaag cgtggcttcc agcttcgctt
    30841 ccagcgtcat gcatgctcca caaacaaaaa atgggcgcaa tgcccatccg ccaaataccg
    30901 ccgactgtca cccgcccgat cctggtgccc ggagccggga tcgaaccggc acgccgattt
    30961 cgcggcgagg gattttaagt cccttgtgtc tacctgtttc accatccggg ctctggaggc
    31021 gggggtcgga atcgaaccgg cgtccacggc tttgcaggcc gctgcataac cactctgcca
    31081 ccccgccatt acactcagcg cacgcgtcaa ggaacacatc aactaaaaag ggaaagcctg
    31141 cagctttccc tttttcgggc tttcccaaaa ctgatggatc gggaaacgag gttcgaactc
    31201 gcgacctata ccttggcaag gtatcgctct accagctgag ctattcccgc atcaatgagt
    31261 tgcgcattct agcacattca ccagccctgt caacggtaaa tgtcagcttt ggctgtcatt
    31321 cgctgctgca cgaacgcttc cacacagcca tccggtaaaa atcaggaccg tttactgacg
    31381 gcgatgcgca ccctcggtta gaatggcggt ttcgatccaa agtggatttc ctgtcgatgg
    31441 acaagctgtt cctgcacgat ttcaacctcc agatgatcat cggtgtctac gagtgggaac
    31501 gcacctcacc gcagaccgtc cacctcgacc tcgacatcgg cctgcctgat cgccgtgccg
    31561 gacagacgga cctcatcgaa gatgccatca attatgctga cgtggccgag cacatccgca
    31621 cctgggtcac gcaacacgag ttccacctga tcgaaaccct ggccgaacag attgcgcaac
    31681 tgatcctgga ccatttcggc gcaccctggg tgcgcgtgag cgccaccaag ccagccgtcg
    31741 tgcgcggcgt gggcagggtc ggcgtgagca tcgaacgcga atcgtcggcc ggctgatacc
    31801 ccgggaaccg ccggttcatt cctccatgac ccgcgttgcg agtcaggtcg aatcagacag
    31861 atcgcggtgc atacagcccg gctcgtagcc ggggctacgc tggcaggccg tacagggaag
    31921 caggcaccca tgcggccaca accgcttcgt gggtcacggg cacgacaatc cggtggggca
    31981 agggcggatg aactgtcgcg ccgtacctct tgtgtcagag aatacccctg actgcgaacc
    32041 acgccttgcg gttcatccag cgaagcgacg tcgcagccgt gcgggggggg ccgaatcagc
    32101 gggttcgcta cgctcgcggg tggtggcggg cgtgcagctg cctgaggcgc tcacgcgcca
    32161 catgcgtata gatctgcgta gtggaaatgt cggcatggcc gagcaacagc tgcaccacac
    32221 gcaagtcggc gccatggttg agcagatggg tagcaaacgc atgccgcagc acatgcggcg
    32281 agggcagttt tgccagcccg gcctgggctg cacgacgctt gatcatgtac cagcaggcct
    32341 gccgggtgat tgccgcgcca cgcgcactga tgaacacatg ctcactggca tgcgcgccaa
    32401 gccaggcgct gcgcccctcg cgcagataac gttccaccgc cagcaccgcc acttcgccca
    32461 gcggcaccaa tcgttccttg ccccctttgc ccatcacctg cactacaccg gcctgcatgt
    32521 ccatctgtcg caaacgcatc ccgaccagct ccgaaacacg cagaccactg gcatacaaca
    32581 tctccagcat ggcccgatcg cgcagcgcca gcggatcatc cccgtgcgcg gccgccagca
    32641 gcgcctccac ctcctgctcg ctaatgctct tgggcaggcc gcgtggcaac ctcggcgcat
    32701 ccagccctga cagcggatgc gtcgtcagca ggcgttcacg gactgcccag ccataaaaac
    32761 gacgcagcgc cgacatcgcg cgcgcggtac tgcttgggct gacacgccgg gtgtggcggt
    32821 gcagcaggta acgttcgatg tcgggacgcg acaccccgct cagcggcgca gcgacccaga
    32881 cagcaaactg cgtcaggtcg cggcgataac tttccagcgt gttgcgtgac agcccgtgtt
    32941 cgagccagag cgcatcgcag aaccggtcga gcagttgctc ctgctcaggc gaaagggtgt
    33001 tcgcagcttc gctcatgcgc cagcaaccag ttcttgtaac cgagcgcctc gtcgccacgg
    33061 ctgtgcatga agccccccat cccgctgcgc gacaccacgc ggtgacatgg caccaccagc
    33121 ggcagcggat tggacccgca tgcctggccc agcgcccgcg gactgctccc cagccgggcg
    33181 gccagctcgc cataggtacg tgtctgaccc ggcccgatgg ttgcgatggc ctgccacacg
    33241 cgctgctgat aatccgtgcc ctgcgcacga accggcaccc cggcgaaccc ggcccggctg
    33301 tccagccaat aagcagccag cgcctgttcc agccgcccca ctgccgcagc ggccgccgct
    33361 tcggcagcct gcaacggccc atccggcgca agaaacccga tgcgcgtcac cgcgccatcc
    33421 gcgacgcgca cgccaagccg tccgaacggc gcagcgagga tcgcctgcca gccgtcagcg
    33481 ttccgcatcg gcgttggcct ggtgtacggc atcctgcgcc atggcacgaa atgcctcatc
    33541 ggaacaatgc tcgacgacac cgcggtccac ccgcaacgca tcatccgtca gaccggcggc
    33601 acgcaggctg tccgccgcat cgagcctgcc ctgccacagc atcgcgtcgg caggcgtgcc
    33661 ctgcgcggcc acgaacagtt gccagcgcgc agcatccagc ggatgcccgg cacctgccag
    33721 cacggcaagc cgggatgcaa tgacacgctg ctgcgccgca tctccggcag caaacgcgcg
    33781 ttcgagccac gcctgcgcgg ccgctgcctg tccgttctga cgtgaccact gcagcacgcg
    33841 cagcaacacc ggccagctgt cgctgctgcc catcgcgccc gcctggtcca gccaggccga
    33901 aaccgattgc gccacggcag gccaatcgcc cacccgggca aacaccgcgg tgcgacgcgc
    33961 ctgccacagc gcggcatgtt cgcctggcgg caggatggcg gtatcctgcc acagcgcggc
    34021 cgcctgcggg tattcacccg ccagttgcgc cagacggccc agccggtaac gtaccggtgc
    34081 cgagaacgat gatgcggcca accccggcca tgccgcctcg aacatgcgca gggcactgcg
    34141 gccggcatgc gctgccagca tctgctccag ccactgctgt tgcgcctggt cccgcagcgc
    34201 cgggtcttgc gcctcatgcg ccagatgcca ccagatcgcc cgcgccatca ccggatcggt
    34261 ggcctgcaca tcacgcgcct gatcggccca gccggtatcg gagccgaaca gcagcagacg
    34321 cacattggca aaactctgcg tcaggccatg gtatgccttg cccagcgccg ccgcatccac
    34381 gccacctgcc ggcgtagcca gattgagcgc cagttcgtcg tagcgcacct gggtgacgtc
    34441 atcatgcaat gcaaccaccg gcctggccag caacgacagc acgcgcgcgt cgggccgcgc
    34501 atcgagcagc tggccgatcg cctgacgcac cgatttatcg tcctgctgcg tatcgcaggc
    34561 atcaatgagc caggccagcg gatcgtcctg cgccagggca gctcgcaccc cggccagcga
    34621 ctgcccctga cccgcctcaa gcgccgcgag caccacgtcg cgacgcaacg ccggatcggg
    34681 gccaaattcc tgctggtcgc gcaacagcag actgactgca ttcgcatccg gctgtggcag
    34741 caaatggctg cgtaccacct gcgcccgcaa gccgggaaag gcgtcggatt gtggcggcag
    34801 ccgccagatc aggttttgca ggtatgtgcg tgccaaaggc ccgtcgcctg catgcagtgc
    34861 gtgcgtcgcg gcaagcgtcc acacctgacg cttcaggtcg tcgcccacat ccgccggcaa
    34921 ggcagcggca tggtcgagca cttgccggtc gctgccgcca agcgccagcg cgcgccagac
    34981 cagttgcagc tgttcgctac gcgccggatc ggccggggga acaggcaaga catcgagctg
    35041 tcgcagagcc agctgggcag caccggcgtc gagcgaacgc cgggcatcgg aaagatcagc
    35101 cgccagtgtg ccggcagcct gcgccagcat aacgaatccg agcagaaaac gggtaatgtg
    35161 catggttttg gcgcaacagc cttttgagtt agaatggctt tttggaatca taaactgttc
    35221 cccgatggat attaaaacct atatgcagcg gcttgggcaa gccgcgcgtg ccgctgcgcg
    35281 cgaaattgcc agcgccgaca cccgcgccaa aaaccgggcg ctggaagcaa ttgctgccgc
    35341 gatcgaacgc aatcgcgagg cgctgctcgc cgccaaccat caggaccttg atgccgcccg
    35401 cgcaaacaac ctcgattccg ccctgctcga ccggctgacg ctgaacgaaa aaggcattgc
    35461 cggcatggcc gaaggcttgc gccagattgc tgcgctgccc gacccggtcg gcgagatcaa
    35521 cgacctcaaa taccgtccgt ccggtatcca ggtcggcaag atgcgcgtcc cgctcggcgt
    35581 gatcggcatc atttacgaag cgcgccccaa cgtcaccgcc gatgcggccg ggctgtgcct
    35641 caagtcaggc aatgccgcca tcctgcgcgg cggttcggaa gcactgcaca gcaaccggct
    35701 catcgccgcc tgcgtacgcg aggggttgat ccagtccggg ttatcgcaag attgcgtcca
    35761 ggtcatcgac accaccgacc gcgccgccgt cggcgaactg atcaccatgc gtgaccatgt
    35821 cgacgtgatc gtcccgcgcg gcggcaaggg gctgatcgaa cgtctgatcg ccgaggcgcg
    35881 tgtcccggtc atcaagcacc tgcacggcgt ctgccacgtg tacgtcgatg acagcgccga
    35941 cctcgacaag gccgtgcgca tcgccgacaa cgccaagacc agccgctatg gcacctgcaa
    36001 caccgcggag acactgctgg tggccgcatc cgttgcgcag cacgtcctgc cgcgcatcgc
    36061 cgccatctat cgcaccaagg gcgtggagat gcgcggctgc cctctcaccc gcgaacag
//
LOCUS       DAIANL010000078         8119 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2342965, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000078 DAIANL010000000
VERSION     DAIANL010000078.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8119)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2342965"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1576 a         2288 c         2608 g         1647 t
ORIGIN      
        1 ttcttctctc cggaaatggg cggctggttc ctcgagccgg acaacttcat tccggccaac
       61 ccgctcaaga cgccgcctga aatcgtgccg ctgtggtact tcacgccgtt ctatgccatc
      121 ctgcgtgcga ttccgagcaa gctgggtggt gtgatcggca tgggttccgc cgtgctgatc
      181 atggccttcc tgccgtggct ggatcgcgcc aaggtgaaat cagtccgcta ccgtgggccg
      241 atcttcaaga ttgcgctggt attgttcgtg atcagcttca ttgcgctcgg ttacctcggt
      301 accaagccgc ctacaccggt gcataccctg attgcccgtg tgtgtgctgt ggtgtatttc
      361 ggtttcttcc tgctcatgcc gtggtatacc tcgatcgaca agaccaaacc tgtgccagaa
      421 aggattccgg gataatgaag aaatttcttg ttgcgctgct gttcgcgcca ttgacggctc
      481 ttgcatccga aggggtgtat ctggaccatg cgccggtcaa cctgggcgat aatgcatccc
      541 tccagcgcgg ggcgaagatg tttaccaact actgcctgag ctgtcatagc gccaactaca
      601 tgcgctataa ccgcctgcag gacattggcc tgacggacaa gcagatcaag gacaacctga
      661 tgtttgctgc cgagaaggtc ggtagtcgca tgacggtggc aatgcgtcct gacgacgcca
      721 agaagtggct gggtgctccg cccccggacc tgacggtgat cgcccggtca cgtacgccgg
      781 actggctgta cacctacctg cgcagtttct atctggacga cacccgtccg accggatgga
      841 acaacgcggt gttcgacaag gtgggcatgc cccacgtgct gtggacgctg caaggcgaac
      901 aggtgctgaa gaaggccggc gaggatggca gcaagcccga gttcgagctg gtcaaacccg
      961 gcaagctcac acccgcccaa tacgacgctg cagttggcga cctggtcaac tacctgacct
     1021 ggatgtccga gccggcgaag atgaagcgga tggaagtcgg cgtgtatgtg ctgctgttcc
     1081 ttggcctgtt ctttgtgctg acattcttcc tgaagaagga attctggaag gacgtgcact
     1141 gatccgcttt ctgtgatcag gcgtgacacg ccccggcaga ccgcacggat acggcaagag
     1201 ccgcaaccgt gccggactgg ccggggcgtg tcctttctgg taacataagc ggtcgggttt
     1261 cgccggcgac gtgttcgtcg gccttatttt ttgatgtctg gggtaatcga catgatgacg
     1321 ttgtactcgg gcaatacctg tccctacagc cagcgatgca ggatcgtact gtatgaaaag
     1381 gggatggatt ttgaagtgat cgatgtggac ttgcacaaca agcccgaaga ccttgccctg
     1441 atgaatccct acaatcgcgt tcctgtactg gtggagcgcg atctcgtttt gtatgaagcg
     1501 aatatcatca acgaatatat tgacgaacgt tttccgcatc cgcaactgat gccggctgat
     1561 ccggtcatgc gggcccgggc gcggctgttc ctgtttacct ttgagcagga tctgttcagc
     1621 catgtcaatg tgctcgagca tggcaacaag acggcagccg acaaggcgcg tacgactgtg
     1681 cgcgataatc tcacgcagat agccccgatc tttgccaagc agaaattcat gctcaacgat
     1741 gaattttcca tgctggacgt ggcaattgca ccgttgctgt ggcggctcga atactatgac
     1801 atccagctac cgcgtcaggc ggccagcctg ctcaagtatg ccgagcgatt gttcagtcgc
     1861 ccggcattca tcgatgcgtt gacgccgaac gaaaaggcca tgcgtaaatg aggccgcagg
     1921 cgcactacgg acagcatggg gcgtaggcga tggagcccgt gaccaccaag ccatatcttg
     1981 tgcgcgccat tcatgagtgg tgcagcgatc aggggtacac tccgtacctg gccgtacgcg
     2041 tgagcccggc tgcgcgagtg ccggtggaat acgtcaagga cggtcagatc gtactcaacg
     2101 tatcgcattc ggccacgcac ggactcttga tggacaacgt gtggattcgc ttctcggcac
     2161 gcttcaacgg tgcgtcacgt gccatcgagg tgccggtgga cgacgtagtc gcgatttatg
     2221 cgcgcgaaac gggcgagggc atgtcgtttg tgcccggcga agtgaatggc agtgccggct
     2281 ccgatgcgaa cgagccaccg cccgaaccgc cgtcggggcc gacagcgccc acacgaccaa
     2341 aactgcaagt ggtcaaatga tagcgcgaaa cggccactgg gcagcgtctg gctccaggcc
     2401 ggccggaacc tgcagcgtca gcaacaccgg ccggttctga agtgcgtcgg ttcggttgca
     2461 tgttcagtcg cgcagcgcgt tgctgatccg gggttgccgc tgtgcgctca gcggtcatgc
     2521 agacgcggtt gcagcccggt caggcctcgg caatgaggtt tttcggtagc gcgaacacga
     2581 cattttcctc gatgcccggc aattcgcgaa cctcgcctgc accgtagctg cgcaactggt
     2641 cgaggacagc cctgaccagc acctcgggtg cagatgcgcc tgccgtgacg ccaatacgcg
     2701 ttttcccttc cagccagctc gcctcgattt gcgttgcatc atcgaccatc cgtgcgacaa
     2761 tgccctgatg ctgggcgacc tcccgcaggc gattggaatt cgagctgttg ggcgagccga
     2821 ccacgatcac caggtcgcat tggccggcta gtagtttgac ggcgtcctgt ctgttctgtg
     2881 tcgcgtaaca gatgtcgtcc ttgcgtggtc cgacgatgtg cgggaagcgc gcgcgcagcg
     2941 ctgcaatgac ctgggcggcg tcatcgactg aaagtgtggt ctgcgtaacg aaagccagcc
     3001 gctgcggatg ggcaatgtcc agcctggcga catcctgcgg tgtttccacc agatacatgc
     3061 cgtcgctgga ctggcccatg gtgccttcca cttcaggatg accgcggtgg ccgatcatta
     3121 caatctccat gccctgagcg cgcatgcgtg ccacttccga gtggaccttg gtgaccagcg
     3181 gacacgtggc gtcgaatacc ttcagaccca gtgattctgc ttcgcggcgt acgtcaagcg
     3241 atacgccgtg ggcgctgaaa atcagggtgc tgcctgccgg gacttcctgc agcgattcga
     3301 tgaaaaccgc tcctttggat ttgaggtcgt tgacgacgaa actgttatgc acgacttcat
     3361 gacgcacgta gatgggagcg ccaaactgtt cgagtgcgcg ctcgacgatg gcaatggcgc
     3421 gatcaacccc ggcacagaaa ccacgcggat tggcaagaaa aatgggtgtc tggctcatgg
     3481 ttggctgtcc tgcaacggtg agacgtcaga agggcgcttg cgcaggctgt cccagatcag
     3541 cagggccgcc ccggcgtcga tggccgaatc ggccacgttg aaggcaggcc aggcatgttc
     3601 gtggatgtga aaccagagaa agtcgacgac gtagcccaag gttacgcgat cgatcaggtt
     3661 gccgagtgcg ccacccagca ccaacgcaag tcccagtttg gccatcagtc ttccgggtcg
     3721 ttgcagcaaa taaaggatga ccaggctgac accgatggca atggcgctga acagtccgcg
     3781 ttgccatccg gcctgatcgg cgaacaggct gaacgcggca ccggtgttgc gtgcgtagag
     3841 cagggaaaag aaggacgtga ccggccattc ctcaccggga atgaaatgtt gggtgatcca
     3901 gtatttgctg agctgatcga gcaggatcac cagcagggca agcatgagtg ccaggcgacg
     3961 ttcagacatg gtggcgtact tccgcagagc cgaacaggtt gccatggcag cggctacaca
     4021 gctgcggatg atccgcatgg ctgccgacgt cggcacggta gtgccagcag cgctcgcatt
     4081 tggcgtgcgc gcttggatgt gcctcgactt cgattacacc atccgcagtc gccgccgggg
     4141 aaagtcccgc cgccgacgtc agcatcgcaa agcgcagttc gtcgcccagc gttgcaagcg
     4201 ccgcgtggtt cgtggcatcg acgtgcagca tgatctctgc ttgcaacgac gagccgatcc
     4261 tgccttccat gcgcagcgtt tcgagctgct tgcgcacttc ggccagcact gtgcggatgt
     4321 tgtcccagcg cgcacccaac gcatcgctat cgacaggttg cggaatgtcg tgccacaggt
     4381 gacagaacag gctgtcgctg tcgtcatccg acagcagctg ccagacctct tctgcggtaa
     4441 agctgagaaa cggcgccatc aaccgggtga ctgcatgggt gatatggtac agcgcgtttt
     4501 gcgccgagcg gcgtgcggcc gcatcgggtg cgctggtgta caggcgatcc ttgaggatgt
     4561 cgaggtagaa gctgccgagg tcttccgaac agaaacggtg cagcagttgc acgatcgggt
     4621 ggaattcata ctgcgtgtag tgttccaggc aggcgcgctg gaagcggtcg agcatgacca
     4681 tggcatagcg gtcgatatcg agccaggtgt cgaccggcat ggcgtcgcgc tgcggatcga
     4741 aatcggcgat gttggccagc aggaaacgca gcgtgttgcg cacgcggcgg tagctgtcgg
     4801 tgacgcgttt gaggatttct tcggataggg acatctcgcc agagtaatcg gtggatgcaa
     4861 tccacagccg cagcatgtcg gcgccaagcc ggttgcagat gtcctgcggt tcgatgccgt
     4921 tgccgcgcga tttggacatt ttgtgtcctt tttcgtcaac ggtgaagcca tgtgtcagca
     4981 gtttccggta gggcgcgcga ccgtccatgg cgcaacccgt gagcaggctg gattggaacc
     5041 agccgcggtg ctggtcagag ccttcaaggt acaggtcggc aggccaggcg agttcggcgc
     5101 gctggcgcag cacgctgaaa tgggtcacgc cggagtcgaa ccacacgtcg agcgtatcgg
     5161 tgatcttgcg atagtcggcg gcttctgcgc cgagcagcga ttcggcagcc agggagaacc
     5221 aggcttcgat gccggtctgc tcgatgcgtc gggccacggc ttcgatcagc tcttcggtgc
     5281 gcggatgcag ttcacccgtt tcgcgatgga tgaacagggg catgggtacg ccccagttgc
     5341 gctggcgcga aacacaccag tcgggtcggc tggcgatcat gccggtcagg cgcgggcggc
     5401 cccaggcagg atagaattcg gtatcctgcg ccgcagcgag tgcgctttcg cgcaaactgg
     5461 tgccagacag accggtgcgg tccatgccga tgaaccattg aggcgtggcg cgaaagatga
     5521 tcggcgtctt gtgacgccag cagtgcgggt agctgtgcac gatgcgctgc cgcgcgagca
     5581 gcaggtcacg ctgttcgagt atctccatta ccgtgtcgtt ggcggtccag acatcctgtc
     5641 cgccgacgaa tggtattgaa gcaatgaaat gaccatcgtc acctaccggg ttgtcgatcg
     5701 gcaggtgata gcgttgtccg acctggaagt catccactcc gtgtgccgga gctgtgtgta
     5761 cgaggccggt gccggcatcg gtggtgacat gctcgccaca gatgagcggt acctgacggt
     5821 cgaggaacgg gtgttgcagc atcagtcctt ccagccgccg accctcgatc tggccgatga
     5881 cttcggctgc atcgaggtcg tagcgcgcga tcgaactttc ggccagatca cgtgccagca
     5941 gcaggatgcc gcgcggggtc agcaccaggt cgtagatcag gtcgggatgc acgcacaccg
     6001 cctggttggc gggcagcgtc cagggtgtgg tggtccagat cacggcacaa gcgttctgtc
     6061 cgcctggcgc gagtccggcc cgatcgagca gcgcattgct gtcggtcaac atgaatgcga
     6121 catcgatggc cggagacttc cggtcttcat attcgacttc ggcttcggcc agtgccgaac
     6181 cgcactccat gcaccagtgc accggttttg cgccctggta caggtagccg ttgcgatgaa
     6241 ttatccccag cgcgcgtacg atttcggctt cagtggagaa atccatggtt cgataggggt
     6301 tatcccagtc tcccagtatg ccgaggcgga tgaaatcggc gcgctgacgg tcgacctggc
     6361 tggccgcgta ttcgcgacac aattcgcgga agcggctggc aggaatgtgt tttccatgct
     6421 gcttttcgac ttgcagttcg atcggcaggc cgtggcaatc ccagccgggt acatagggcg
     6481 catcgaaccc ggacagtgtc ttgctcttga caatgatgtc cttgagcacc ttgttcacgg
     6541 cgtggccgag atggatttcg ccgttggcat aaggtgggcc atcatgcaga atgaattttg
     6601 gcctgccggc gcaggcggcg cgaatcttgc ggtagcgctg cgtggcgctc caatgggcga
     6661 gttgcagggg ttcgcggcgc ggcaggtcac cgcgcattgg aaaccgggta tcgggcaggt
     6721 tgagcgtgga tttgtaatcg gacatgactc aggcaggcag ggaaggggca atcagattga
     6781 agtgttcgcg cgcgcgggcg acgtcgcggg cgatttgctg gcgcagcaac gccaggtcgg
     6841 aaaatttctg ctcgtcgcgc agtttggcga cgaattcgac ttcgatgatc cggccataca
     6901 ggtcggcaga gaaatcgagc agatgcgtct ccagccgtgg ttgttgcgtg gattgcacgg
     6961 tcgggcgcat gccgacgttg actactgccg gcacggcatt cagaccggag ccgcttaccc
     7021 gggcgcaaaa tacgccggac agtggcagcc ggcggcggtt gagtgccagg tttgccgtcg
     7081 ggaaaccgat tgtgcgtccc cgtttgtcgc cgtgcatgac gtgtccgctg atgcagaatg
     7141 gacgtcccag caattgggcg gcagtcgcga aatcgccaga ggcaagcgct gtgcggatgg
     7201 ctgtgctgga gatgcgttgc gttgcgacga cgacgctgtc cattgcttcg acgctgtagc
     7261 cgtgctgttg acccagcagg ctcagcaggg cgaaatcacc ttcgcgtcgt gcgccgaaac
     7321 ggaaatcgtc accgatgagc aggtggcgca tgcacaaacc ggccagcagc atgtcctcga
     7381 cgaactggcg cgccgacatg gcggcgaggc gggcgttgaa cgggcagata tgcacatgat
     7441 cgatgccgca acggtgcatg atggccagtt tgtcgcgcag gctggacagc cgtgccggta
     7501 cgtcgtcgcc gccgaaccat tcgcgtggat gcggttcgaa agtgaccgcc accgcttcga
     7561 gacccatggc atgcgcttcg cgtgacaggc gttcaagcat ggcctgatgc ccgcgatgca
     7621 caccgtcaaa gttccccgct gtgacggcgg ttggcgtggc aaggccggga tggaaaccat
     7681 gagtgatgcg catgagaggg atcgcgatac tggaagcctg gaattctacc cgatttgcaa
     7741 tcggctgtgg caggctatgg ggtggatgtg ctgactgact ggcgttgacc ggccgggaag
     7801 ccgtggcgag aaatatggcc gatggcgggc tatggcgtag taaaatatcc ggacatccgc
     7861 taatgtcgga tggacgggat tgccgcacgc atggcagtgg aattttcctg tgcaacgtgg
     7921 ccgacggtgg gccgcggcag tttttagcag agacaacggg ttcagggctc aatcaatgac
     7981 gcatcaagcg cacaaaccag gagatcacga cctgtcatac gtcgtggaag aaatcgattt
     8041 tgaaccgaag gtggtcgaac gttcgcacca gattcctgtg ctgctcgaca ttggcgcgga
     8101 ctggtgcgca ccgtgccgc
//
LOCUS       DAIANL010000079        16061 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1531228, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000079 DAIANL010000000
VERSION     DAIANL010000079.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 16061)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16061)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16061
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531228"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3400 a         4352 c         5082 g         3227 t
ORIGIN      
        1 tagagtgaca cggtcttgcc gctgccggta gggccggtta ccaggatcat gccgtagggg
       61 cggtggatgg cgttgagcag gagctctttc tgatgcggtt cgtagccaag cgcctcgatg
      121 ccgagtgtgg cgctggtcgg atcgagaata cgcatcacga tcttttcgcc atgcagcgtg
      181 ggcagtgtgc tgacgcggaa atcgatggca cggttgcgcg agagcacgag cttcatgcga
      241 ccatcttgcg gcacacgttt ttctgcgatg tcgagtttgg aaatgacctt gatgcgcgaa
      301 gccagctttt cgcggatggc tagcggcggc tgggcgacct cccgcagtac gccgtcctgg
      361 cggtagcgga tgcggtagaa cttctcgtag ggttcgaaat ggatgtcgga tgcgccgtcg
      421 ttgatcgcgt ccatgagaac cttctgcaaa tagcggacga tcggcgcatc atcgatatcg
      481 acggtatcct cggctggctg ctgcgacgcc tcatccgagg aaaaatcgag gttgaggtct
      541 tcggtattga tggccgacag gacgttggcc tcgctctcgg tgatctgcgt gatcagtttg
      601 ccgagcttgt cgtcttccac cacgacgatg tcgacggcca ggttggtctg gaacttgatc
      661 tcgtcgaatg cctgcaggtt ggtcgggtcg gaaaccgcga ccgccagccg gttgccgcgc
      721 tggtagagcg gcagcacgcg ccggccttgc atcagtttgg cgtcgagggc ctttttgggc
      781 agcacctcgg ggtcgaccgc agccagattg aggcagggga agccgaactg taccgcggcg
      841 aattccgcca gacgtgaagc tttgatcttg tgtgctgaaa tcagcagcga cacgaaatgt
      901 tcctcgccgt ttttcgcttg ccgcatcagg gtgtcggctt cttcttcggt cagccagcgt
      961 tgcgtgacca gtgcgcgggc aagaccggta agcggggcgg tggtgggtgg cgtggctgac
     1021 atcggcgtat cgtattcgtt aggttccgtg aatcaacagg gtggtgttga aaaatcggtg
     1081 gcgttgcggg cgctggcggg atgcggcacc aagcggcttc ttctctcccc accgtacgcc
     1141 atgccggcag gcgcgtggaa ccggatgctg acaacagcgg tcattcgcgc aacgacaggc
     1201 caggtgcaac gtcaggctcc aatgatgcgc tgagcgggcc gttttgtaaa caaggcgatg
     1261 aatgtggatc aggtcgaccc gttgggtgcg gtgatcgcca cgggtcagga cgtcagtcgg
     1321 gggaagatgc gcgcggtctt gatcatgcgt tcctgcacct ggatgatctc gatgggatag
     1381 ttggcgattt tcaccgtggt gcccacttcc gggatgtctt caaggtgttc gaggatgatg
     1441 ccgttgaccg tcttcgggcc ggtttcgggc aggtggagcg ccagccggcg gttgacgtgg
     1501 cgtacgctgc ttgcgccatc gaccagccag ctgccgtcgg cctgctcctg gaaaccggac
     1561 aggtccggcc cgccgccgta ttccagttcg ccgaccagtt cgcccaggat gtcatccagg
     1621 gtgatcagcc cgagcagttc gccgtattca tcgaccacca ggccgaaatc gagcttgcgc
     1681 gcctgaaact gtcgcaactg ggtgaacagc gctgcgcccg acgggatgta atagggggtc
     1741 tggaagtggg attccagcgt tgcggcatcg atctcgccgc tgtgcagttc tcgggtgagt
     1801 gcgcgcacat ggacgatacc gatgatttcg tcgagttgct cgcggtagac tggcagacgt
     1861 gtgtggtggc tgctgatgat ctcgcgcgtg attgattccg tgtcctgggc gatgtcgatg
     1921 gcttcgatct ggctgcgcgg cgtcatcaca tcgttgaccg tgctgtcttc cagatcgagc
     1981 aggttgagta cggcgcgttt gtgtgtggcg ggcagcaatt ttccggattc catgaccagc
     2041 gtgcgcagtt cctcgatgcc gagcacgtgt tcgccctgtg cgccggggtt gagacgcatc
     2101 agtcgcagca gtccctgaac gaacaggttg acgaaccaga cgatggggta ggccaggctg
     2161 agcatcggtc gcaggatgaa actggccggg aaggcgattt cgtccggata ggcggcgccg
     2221 atcactttgg gagtgatttc gctgaatacc aggattgcaa aggtcacagc cagcgtcgcg
     2281 acgccaagtg cccatttgct gtcgccgaac aggcggatgg aaatcagtgt cgcaagcgtg
     2341 gccgaagccg cgttgatgag gttgttgccg agcaggatga cccccagcag cttgtcagtg
     2401 tgtgcgagca ggtcagcggt gcgttgcgcg gcgatgatgc cgcgcccggc caggtggcgc
     2461 aggcgatagc ggttgatggc catcatgctg gtttccgacg ctgaaaaaaa ggcggaaagc
     2521 agcagcaaca cgaaaagcag cagcagcagc gcgcttaagg ggatgtcgtc caaaatcgga
     2581 ttctgggtga atggattcgg ataggttcag tatcagtgtt cgtcccaggc atgtcaagcg
     2641 ccgggacggc ggttccggcc gctccgtcgg gaacggccgg caacgggtta cttgccgacg
     2701 tggggggcgc tggagtagcg ttccatcacg gtgcggtctt tccacagctt ctcgtcaaga
     2761 atcgcggaag ggatggtgta ctgggattcc ttgtcgaccg ggttggtctt cacgatgcgc
     2821 acgatatctt cgtagccgat cttgtgcagc aggccgctct tgtccgtcat gcgggcaatc
     2881 atggcctggg caaatgtctt gtagacgtaa atgtttgcgg gacgcagctt gccgtccggt
     2941 gcgcgccagg taatggtata gcgggtgttc tcctgaaaac tttccttgtc cattgtgtgt
     3001 gttctccgtt aacgtggtgc agagggtaaa acgatgtaaa gcgcccgaaa ccggccagcc
     3061 tggacatggc cggggcgact tgccatgcga atttagcatg gaacgacaag cgcgtaaact
     3121 tgtcatacga ttgcaaaatt atttgcacgc ggggcgtaat ttggtatatt agcatccttt
     3181 caaagttgct ttgagatggc tatgtttggg ttcaaggaag tggacgtgca ggggttggag
     3241 cggttgcagg aaaactccgg atgcctgctg gtcgacgtcc ggaccgatgc ggaggtttcg
     3301 cggggcggta tcggcggagc catacatatt ccgctgcatt tgctgccaat gaaagtgcag
     3361 gatctgccgc acgacaagcc gatcgtgttc tactgccagt cgggggggcg ctccgctcag
     3421 gcgtgcgcgt tcgtcgcttc caggggattg aatgatgtgt ataatcttca gggtggcatc
     3481 atcggctgga tccgtaccgg aaaaccgctc gcggcaattt gaaaggctga ctttctgaac
     3541 ggcaaagccg atttttttct atacctgaga atgatggaga cgagtaatga atttcgacaa
     3601 ggaacttgat gcacgcggtc tgaattgccc cctgcccatc ctgcgctcca agaagagcct
     3661 gggcgaactg accaccggtc aggtgctgaa ggttgtggcg accgatccgg gcgccatcaa
     3721 ggacttcgag gcgtttgcca agcagaccgg caatgagctg ctgtcgtcgg cccaggctgg
     3781 cggcgagttc accttcttca tcaagaagaa ataacgacct cttccggccg ggcgcgcaca
     3841 gcgccccgtt catgccggca gccgcagggt tgccggcgca tgacaacaaa aaaatgagga
     3901 gatgtcatga gccaaaagaa actcgcgatc atcgctacca agggcacgct tgactgggcg
     3961 tatcccccct atattcttgc ttccacggcg gcggcgctcg actacagcgt tgaagtgttc
     4021 ttcacgttct acgggctgca gctgatccgc aaggatctgt ccgccctgaa ggtcagcccg
     4081 ctgggcaacc ccggcatgcc gatgaagatg ccgttcggac cgaaatggtt tcgcgacatc
     4141 acctgggatg tgccgaacat catccaggcc aatatcccgg gttatgagtc gtttgccacg
     4201 gcgatgatga aaaagaccat cgccaacaat ggtgtggaaa cggttgaaaa catgcgtatg
     4261 ctgtgccagg aagccgaggt caagttcatc ggctgccaga tgacggttga gctgtttggc
     4321 ttttcgcatg acgacttcat cgatggcgtc gaatatgcgg gcgctgcgaa atttttcgaa
     4381 tttgctggtg aatcggacat ttgcctgtat atttgataga attggtaggg tattctcgcc
     4441 acgctcggct ggccagggtt cagtcggggt ggtgaaaggc ttctgaagca tgtgaggtgt
     4501 gccggcgcgg gcgcatcatg cgagatggcc ggaaacaaaa tttccggagg attgcagcaa
     4561 cggtctggcg catcggccag gctgtcttga gtcgccgttg ttgagcaacg gttccagatc
     4621 acgttcgtcc cgcaaggctg cgggtcggct tcctgccgcg caacgggaag gcaaggctgc
     4681 cgttcgggcg ccggtgccgg tgttaacgat tactgctaga taaccactga ggtgcataat
     4741 ggctacgtac gctgaaatgc gcgagattta tgatgatatt cgcggcgatt tccgcttcga
     4801 tcacgagctg aacggctgtc tgaattgcgg gatttgcaca gccacgtgcc cgtccgccca
     4861 attttacgat tacagcccgc gcgaaatcgt gcaactgctc tggacggaaa atattgagca
     4921 gatctacgac gccatgcagg aaaagatctg ggcctgtgcc cagtgcatga cctgcgcggc
     4981 gcgctgcccg ttcaagaatt cccctggtgg gctggtcatg atcatgcgcg aggtgtccat
     5041 caagcatggc atgcagtcgg ccaaggatgt gttgcgtccg ttcggacgcg tgatgctcaa
     5101 gctgatcacg acgggcaacc agctttcgcc cgacatgatt cagcccgacc atttccctga
     5161 ttggggtccc aatatccaga aggtggaagg cgacctcaag acgctgcgca aggcgattcc
     5221 cgtgcgcacc ctgcagacaa ccgatactgc ttgggaagtt tcactgaaaa cctcggtgga
     5281 aatgtatacc atctgggaga tgaccggcgt gttgtcctcg ctcgaagcca tggacgaaaa
     5341 cctgtttgac gtcattgaag acttcattga cgaaaagcgg gaagagtacg aggaatggct
     5401 ggaagagcag gaagaagact aaacggatgc gcaagcgcgc atcgactgga catttgacat
     5461 tcaggagata gaccatgagc gagatcaata gcagtgacag ccagggtatc gcaggacacg
     5521 gttcgttctt tcagcagacc agcctgtccg gcgcagatgc cacccgagcc accgaatggg
     5581 tgcgcaagca cgtggacaag cgcaccatcg atctgggtga gcggatggac gacattcgcg
     5641 agcacatgtg ggaacacgaa aaggacggtc agatcatcgt gcaccgcatc accgatgcgc
     5701 acgacccggt agaagtgcag acgctgtttg gctggaccaa gcgcgtaccc acccgcaagc
     5761 tctggcatca caagtcgtgc ggtcagtgcg gcaatatccc gggctacccg accagcatca
     5821 tgtggttcat gaacaatttc ggtttcgtcc ccggcgagga ctacctcgat gaaaccgatc
     5881 agacctcctg caccgcatgg aactaccatg gttccggcat cggcaacgtc gaatcgctgg
     5941 cagcggtgtt catgcgcaat ttccaccagg cctacatttc cggaacccag cacggttacg
     6001 aggcaggtca tttcttcccg ctggttcact gcggtacctc cttcggcaat tacaaggaaa
     6061 tccgcaagta tctggtcgaa tcggctgaac tgcgcgagcg cgtgaccaag attctcggca
     6121 agctcggccg ccttgtcgac ggcaagctgg tgatcccgga agagatcatt cattactccg
     6181 agtggctgca cgtggtgcgc aagcgcattg cgtccgatct gcagaagatc gatgtgtcga
     6241 acatccgggt aacggcccac gctgcctgcc actattacaa gatggttcat gaagatgcca
     6301 tctacgatcc cgaagtgctt ggcggcaacc gcaccgcggt gggtacctcc attgcacagg
     6361 cgctcggagc gcaggttatc gattattcga cctggtacga ttgctgcggc ttcggtttcc
     6421 gtcatatcat ttccgaacgc gagttcacgc gctcgttcac catggatcgc aagatccgtg
     6481 tcgcgcgcga agaagccaat gcggacgtga tgctgggcat cgataccggc tgcatcacca
     6541 ccatggacaa gaaccagtgg atcggcaagg cgcacgaaaa caatttctcc gttccgatca
     6601 tggctgatat ccagttcgcc gcgctggcct gcggtgccga tccgttcaag atcgtgcaac
     6661 tgcaatggca tgcttcgcct tgcgaagagc tggtggaaaa gatgggtatc tcctggactg
     6721 acgcgaagaa gaacttcgaa gcctatctga aagaagttga agcaggcaac atcgaatatt
     6781 tatataaccc tgagctggca ttaggaggcc attgataaga tgtctgatac tgtattggta
     6841 gtgggcgcag gcccaaccgg gttgtcggcg gctgccggtg tggcttccat cggcaagaaa
     6901 gtcattctgg tcgaaaagga ggctgttctt agcggtgcgc cgatcctgtc gggttatgcc
     6961 aagctggtgc cgtccggcga gtgggcgaag gatgccattg gacgcatgat tcatcgcgtc
     7021 gaaggcaacc ccaacatcac catcctgaag tccacccgcg tgaccaaggt ggatggcgag
     7081 atgggtaact tcaccgtgac gttctcgaac ggcaagaccg agcaggtggg cagcatcatc
     7141 ctcggcacgg gctttaccca cttcgactcc atcaacaagc cggagtgggg cttcggcaca
     7201 ttcgaggatg tgctgaccac tacccagatg gaacagatgg tcgccagtgg caagattgca
     7261 tgcccgtcga acggtcgtgt accggaacgg gtggccattc tgctgtgcgt gggctcgcgt
     7321 gaccgtcaga ttggtcgcga atggtgttcc aagatctgct gtaccgtgtc gaccaacctc
     7381 gccatggaga tcaaggaact gtcgcccacg accgaagtgt tcatctacta catggacatc
     7441 cgtacattcg gcctgtatga agacaagttc tactggaagt cgcaggaaga attcaagacc
     7501 aagtttgtca aggcccgtat tgccgaagtc actcgctccc ctgatggccg tctgctggtc
     7561 aagggtgaag acacgctggt caagcgcccg atcgtcatcc ccatggatat ggtcgtgcat
     7621 gcagtaggca tggatccgaa cgaagaaaat ccggacatcg ccaaggtgtt tggcatcggg
     7681 ctggaaaagc atggtttcat cgagcgtggc gagcagtaca ccagcacgtt ctccagtacc
     7741 cgccgtggtg tttacgtcgg cggatcggcg ctgggcccgg aagatatcga caccagcatc
     7801 tcgcacggcc tggccatggc gatgcgcgcg gtgggcgaac tcaatgctct ggttcaaaaa
     7861 gcagcctgac aaggcggctg cggcgcaagc ccaggagcag atcgttcctg ccgatctggc
     7921 ggtgccgctt gacccggtgt actttgcgca gcggttctcg tcattgcagc ggcatgctgc
     7981 agacgatggc ggtatcgagc caatggtagc ggccctgggc gccaagcagc agctgttcgg
     8041 caggctgctt gatgcacagg tgctggccag tctggatgcg gaagggctgg aaattttgct
     8101 cgacaccgtg ttttcggcgc gacgacgcgt gtttccgggg ttcatggaac atggaatgga
     8161 gcgggtgcgc gaagccataa gtgaactgct gcacggtcgc gagcacgtga cccggcgtat
     8221 ggaagcgttt tccatgctgg tgccggtgga tgagagcggc gatcgggagt cgcgcaagca
     8281 ggcgctgaag acacgccggg ccgcattcga ttttgctgcg gagatcctgc atttcagcca
     8341 gccagagcgc tatccgctga tgagccggtg ggtatgggat cagcagacgc aaagcggagc
     8401 gttgcgcgaa ttcattcgcg gcaacgacag catgtcagac ctgcggctgg gcagcgctcc
     8461 ggaaatgtac gaaggtgcgc gtgtctggct gattgcgcaa ctgggtgagc aagggctgta
     8521 ccgtgatctg ccgttctggg tggacctggt gctggcacag gcgtacaccg actatttccg
     8581 ctcgctggcg gaagggatgc tgagtgcgga attcggtcgt ggccatatgc ccgccgaaca
     8641 cgtccgcaaa tttcttggca tcgatgcgcc ggccaaagcg ggaatgtcca gggtgaggcg
     8701 tgcgccagac tgaaaccaga aataggtggc gggacagaaa cctgacgcga gacaaggaaa
     8761 tttaggagag gcttcaatgg cgattcatga aaaaacactg attgatccgg atcgactggt
     8821 ctccagcgaa acgctggtgg tggatggggt cgacgtgtcc ggtcactgga acacgatgat
     8881 cctgccacgc accctgcgcg agtatgatga cgggttcgag gaacacctca agacgtatca
     8941 tggtgcggag aacgtgcacc gttgctggca atgcgggtcg tgcaccaatt cctgcacaat
     9001 gtatgcacag aataccgatt tcaacccgcg ctactggatt tacctgaccc gtctggggct
     9061 gaccagcgaa atcctgaagg acaaggaaat catctggcag tgcgtgtcgt gcaaccgctg
     9121 caccaatatc tgccccaagg acgtgcgtcc ggaaggcgtg atggaggcgc tggcgcactg
     9181 gattgaaacc gagggccatg gtgaaaagac caactcttcc gtcttcgacg aagagttcac
     9241 gcgtcagtgt ctggcacgtg gccggatcga agattcggaa gtcatgacca atttcttcaa
     9301 gaacacccat caggacatca agcagatggc gctgcatggc tggctcggcg ttttcgttac
     9361 gcgcatgagc aaactgacgg aactgaagca gggaaatgtt ttccgcttcc ttgcccgcac
     9421 gcctatcatg cactcgctgc acatggggtt ggcgatgttc ttcaagccca agaccaaatc
     9481 ctggggccgc accggtgaag tgctgaccca gtacgtgaaa gaacagaagg agctggccca
     9541 tggctaaggt tgcctattac ccgggttgcg cgctggaagg atccggcggc ccctacgacc
     9601 gttccacccg agcgctggtc aaaagtctcg gcctggaaat ggaaacagtc aatgactgga
     9661 actgctgtgg tgcgatggaa gtgaaaaaca tccatccgat gctgcagacc tatatgtcgg
     9721 cccgcgtgat ggcaattgcc agcgaacaga tgggcttcga cacggtcatg gcgccatgca
     9781 acggctgcta ccacaacctg aagaaggccg agtacgagct cgccacgtcg aaggaatcgc
     9841 tcaacacgtt gcaggatctg gccaagaaat ccgaagatcc ggtatacaag ggtgacgttc
     9901 gcaccattca cctgctcgaa tggctgatgg aagacctggg cgttgacggc atcaagcaga
     9961 agatcagcaa gagcctcaac ggcatcaagg tggcaaacta ctacggttgc atgtacaccc
    10021 gtccgcgtca gatcttcccg gaaaaggatc aggggccagg aagcgaatcg agctatcgtc
    10081 cgcacttcat ggatgacctg ctgggtgccg ctggtgcggt caatgttgat ttcccgctga
    10141 agacggcgtg ctgcggtggt gcgcacacgt tgtcagactc ggacacctcc acccagctgg
    10201 tactgaatat cctgcaggcc gcggaagatt ccggagcgga agtgatcgcc accgaatgcc
    10261 cgacctgtca ctccgggctt gaaatgcacc aggtgcgtgc ggagaccgag ttcggtatca
    10321 agaccaatgt caagatcctg tactttacac agctgctggg cctggccatg ggattgtcgg
    10381 cacgcaagtt gggcgtgcac gagaacgtca gcgattcgct ggatttcctg cacgaaaaag
    10441 gcgtggtctg acaaacagtc tcaaatgaag cagggaaggt gcgcgagcgc cttcccggtc
    10501 aggtaggcgg atgatggatt gcaatggatg tgagttccgc ccggaactgt attacgatga
    10561 tcagtaccag atctgggtgc gtcgcgagga agacggcagc ctgacactgg gtatgaccga
    10621 catttcccaa acgattgcag gcaaaatact gcacgtgcgg gtgcgacgtc ccggaacgga
    10681 acggctggtg ggcaaacccg tggcgaccat cgaaagtggc aaatgggccg gacctgtgcc
    10741 caatgtgttc gattgcgtca ttgaagaagc caacaccagc gtgctggaag acccgaacct
    10801 gctcaatatc gaaccgtacg aagcgtggat tgcccgcgtt cggccactgg aaggtgtcga
    10861 tgctgcgctc ggggtgctgg tcacaggtga tgcggcgcgg gtcggttatt gcgaacgtac
    10921 gcaacgggaa gacattcatt gcgaacgcgg agcacgttga tggatgcatc gcattatctc
    10981 gacaatgagg aaaaatcagt cgtcatcatc atgacgagtg gcccttctac acctcagcgc
    11041 tgcgcaacgc cgttctatat tgcggccatt ctggcgtcga tggatgccga ggtcagcatt
    11101 ttcctcacca tggagggagt aaaactggcc aaacggggcg tggcggccga gttgactgcg
    11161 ctggagggtg gaaaaaccat ccttgagttc atgcgcgatg ccaaggccgc cggagtaagg
    11221 ctgtattcgt gcaggccagc gatgcccgga catggcatga gcgacagcga tatcatcgac
    11281 gaagtcgatg aaatcgccaa tgcgagtaaa atggctgatc tgctgcttgc gtgcgacaag
    11341 tcgatgtact tctgacctgg cgcgattcga acaaggatca aaaccggcat gaaacacaca
    11401 tggatgtgaa tttacatagt aaacttcgca ttcgcgcgca gactgcgtgt gatgcggatg
    11461 cacacactgc ggtgcgccag atacgggcct ggcggggacc ggccggcaca atcccggtcg
    11521 cacgagcacc aggaacagat ttcaattgca gcatcattaa acagtaagga gaaacgagca
    11581 tggcaacagt acgtggttgc aaccttccag acgacctgta ttacaacatc gataacaatg
    11641 tttgggcacg ccgcgaagcc gatggcagct tgaccgttgg catgactgcc tacgcctgct
    11701 cgctggcagg tgaaatcgtt tcgtatacgc cgaaaaaagc cggcaaggaa atcgagcaga
    11761 acaagtcggt gtgcactgtc gaatccggca agtgggtcgg tccggtcaag gctccggtca
    11821 cgggtgaaat cctcgctacc aatccggacg tggctgccaa gccaggcacc atcaacgcag
    11881 atccatacgg cagcggctgg ctggtcaaga tgaagcccag caactgggac ggtgattctg
    11941 ctggtcttgc aaccggggcc gcagtagcca caggcttcga agcaaagatg aacggcgacg
    12001 gatttgccgg ctgctgagac agtacatata caggaacagt ccgcccggcg gaccgttcca
    12061 gccaagcaat aggggtgatc gttcgcccct attgttttct ggggcacaat aaccattcat
    12121 gcaacgaggt aagcaacgaa tgagcgactg gctgaacatc gtggactgcg tggaaccact
    12181 ggaaggcgcc ggactggatg ccgcactggt ggatgaaatg gaacgcagat tcggcggcct
    12241 gcaaggcgag cgcgccggtc gtgtcaactt ttacacgccg acgttcaaag cctactcgag
    12301 ctcggaaatc gcaggctgcg gcaaaagcgc ctggccggcg gtatcgatca ccggcggcga
    12361 ctgcaaactg caatgcgatc attgcaaggc caagattctt gaacccatgc tgccagcgcg
    12421 cactccggag caattgtggg ctctggtcaa cgagcaaatc gccggtggtg ctcagggcat
    12481 gctgatcacc ggtggttcga actaccgtaa cgaagtcgaa tacgatgcgt attacccgac
    12541 gattcgccgt atcaaggaca cgttccccca attcaggata gccatgcata ccgcgttggt
    12601 ggacatggat atcgcccggc gcatggaaga cagcggcatt gatgcggcga tgatggacgt
    12661 gatcggatcg caggacacca tcacccaggt ctatcacctc aaacgcactg ttgacgattt
    12721 cgaaaagacg ctcgaatacc tcgtatccac gcagctgaaa gttgtgccac acatcgtcat
    12781 tggtctgcac tacggtgaat tgctgggcga gtggcaagcg ctggagatgc tcaggcgcca
    12841 cgttcccgac gcactggtgc tggtggtggt catgccgttc tacgcaaccg gcatgcgtcc
    12901 atttgtcagc ccggacacga cacaggtggg tcgcttcttc atggatgccc gctccgcgtt
    12961 gcccgaaatc ccgttgctgc taggttgtgc ccgtcccgcg ggtcgcgaca agtcgcgcat
    13021 cgacagctat gccgtgatgg caggtctcaa cgggctggcg cacccggccg atggcatggt
    13081 cgaactggct gtgcgtctgc agcgcgatgt ccgcgtcacc ccctcgtgct gctcgattgc
    13141 cgtgggcgac gaagtcatgg ccattgacgg cgcggactcc ggtattcagg tcgatatcga
    13201 tcaggtcatc gaacaggaac gtcagcgccg cgccaaacct gcgggccagc tggcaggcat
    13261 ccgggtggtg accgaacaga aagaagcgct tgccggcggc gggtgctgca gctgatgaca
    13321 gccgttacag acaagcctgt ctggcgcgtc atcgacaccg gtgtgcgccg tgccgccgag
    13381 aatatcgcgc tcaaccgcgc tctgctcgag gcacgccagc agggccttgc gccctgcacc
    13441 ttgcgtttcc ttgcattcga gccgtcagcg ctgctcggat tccaccagag cgcggcgcag
    13501 gaactgaatc tcgactggtg cgcaacgcaa ggtgtagcga tacagcgcag gataaccggc
    13561 ggcggtgcga tctatatgga tcccgatcag atgggctggg aaatctatgc cgatcgcgat
    13621 gccgtcggca gcgcgcagat ggagcacatt gcacgcagga tctgcgaagc tgcggccggc
    13681 gccctgcaga ccttgggtgt ggctgcagcc taccggccgc gcaatgacat cgaagtggat
    13741 gggcgcaaga tttcaggaac tggcggggcg ttcgatggcg atgcgctcat gtaccagggc
    13801 acgctgttgt tgcgtttcga cgtcgagcgc atgttgcgtg tacttaatgt gcctgcggac
    13861 aaactggcgg acaaggcact ggcttcggcc agagaacggg tgaccagcct ggaacagttg
    13921 ctggggcggc tgccgcagcg cgagcacatc cagcaagcac tgacgcgggc gctggccgag
    13981 gctttcgggg tggaattcca ccctggcgga ctgacggacg acgaacagcg gctatatgcg
    14041 caggcgttgc aggagatcga ccacccggac tgggtggact tgatccacca tccggcggac
    14101 gagcggccta cgcttgaggc atcgcgccgc ttccagggcg gtacgctgcg cgtgcgtatt
    14161 gcatgggatg cccagcagcg gcggatccgt cagatctggt tcagcggcga tattttcatc
    14221 agcccgcgtc gtgcgctgat tgatctggaa agcgtactgc gcgacacact gggcgatcgc
    14281 gtcgagcagc aggtactcgg attttttgcg gaccgcaacg ttgacatgct gtcgctgcgc
    14341 cccgaagaca tcgtgcagtt gctgcacgaa gccatggacg tacaggcatg aacccggtcg
    14401 atgtgctggt ggtgggcctc ggcccggctg gcggagcggc tgcggcagcc gcagcggcgg
    14461 gcgggttgag cgtgatgggc atcgaccggc gtgaacagat cggcctgccg gtacagtgcg
    14521 cggagttcat tccgctgccg cttggcagct atgcccagga cgacggcgtg attctgcagg
    14581 ccgtgagcgg catgaagagc tatctgccgt caggcgcagt cgaacagtcg gcgtttcccg
    14641 gctggatgat tgaccgggcg aaattcgatc aggcgctggc aacacaggcg cagcgtaacg
    14701 gcgcgcgact gcacacctca gcgcgtctgg tggcgatcga cagcgcgcag cggaccgcgc
    14761 gaatacacct ggacggtgca gaacacagca ttggctaccg catgctggtg gcggccgacg
    14821 ggccgcattc gcaggtggcc acaagcctcg gctggccggc gctgcaggtg gtgtacacac
    14881 gtcagtacac ggtcgaactg aagcagcgct atgcagatac cgatatctgg ctgtccggcg
    14941 actaccccgg agggtatgcc tggctgtttc ccaagggtgg gctggccaac atcggtctgg
    15001 gcatcgacaa aagtcatgcc cccgagctca agcagccgct cgatgcattg catcgccagt
    15061 tggtgcggca ggggctggtc ggtgagacga ttctgtcacg caccgggggc gcgataccgg
    15121 tgggtggcat gcgcgaacgc ttgatggctg gggaaggcat cgtgttcgtc ggtgatgcag
    15181 ccgggctgac gcaccccatc acaggcggcg gtatccccgc cgcggtattg agcggcgtgc
    15241 gcgcaggcga agccgccgtc gactggctgg tgggcgggcg taccgatgcg ctggaagatt
    15301 acgaacagga catgcgcgac cagtacgagg caacgttgca gcgtgccgtg gcgcgacgcc
    15361 agtggctggc gcaattctgg aataccgatg ccgcgcggga tgacgcggtg catcgtcggg
    15421 gatggattgc cttccctgaa tattttgaac agtaaccgca gcaaaggaga aaccgatgag
    15481 cgcagtgatc gatcaaccgc aagtggtgca attctaccgg ccagataccc atgtcaggac
    15541 tccggagatg cgttcgccgg attacgtgca gatgagcacg gcggcagcca ttacgcttgg
    15601 cctggtgcct ggcagcatgc accgtacctc gtgtacccat tgtcttaatt tgctggtcac
    15661 ctatccggaa ggctgccgcg ccaactgcac ctattgcgga ctggcgcgcc atcgcgaaga
    15721 aaaccgcgac tatgccgacc gcaatttcat ccgcgtggag tggccgacag cacgttacga
    15781 tgacgtgatc gagcgcgtga agcaggggca agaccacggg cagatcgaac gcatgtgcat
    15841 ttcgatgatc actcacccca attccgatgc cgatgcttac gtgctgctgg aaaaatgggt
    15901 gcgtgaagtg ccgcatattc cggtctcgat cctgtccaat ccgaccacca tggaaaagca
    15961 ggatctggta cggatgaagg acatgggggc ggatatcttt accattgcgc tcgatgccgt
    16021 aacgccggag attttcgaac gcactcgtgg caaaacggtc g
//
LOCUS       DAIANL010000080        17262 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2748823, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000080 DAIANL010000000
VERSION     DAIANL010000080.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 17262)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17262)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17262
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2748823"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3497 a         5604 c         4889 g         3272 t
ORIGIN      
        1 actcctgaag tattggtcat gagaatcagt ttctcggcat gcagggtctc ggcaagcttg
       61 ccagccacga gatcggcgtt gatattgtag gattcgccgt cggcgccgac accaaccggc
      121 gcaatcaccg gaatgaagtc gcgactgtcg agcagttcga ccagttcggg atcgacacac
      181 accacctcgc cgaccgatcc gatgtcgagc ttgttgcctg catgctcgcg gtcatcgatg
      241 aacaacttgc gtgcacggat catggcgccg tccttgccgg tcaggccgac cgccttgcca
      301 ccgtgctggt tgatgaggtt gacgatttcc ttgttgacca ggccgccgag caccatttcc
      361 accacatcca tggtttcccg gtcggtcacg cgcatgccct gcacgaactc gctctgcttg
      421 ccgacgcgct gcagcagttc gccgatttgc ggcccgccgc catgcacgat gaccgggttc
      481 atgcccacca gcttgagcaa caccacatcg ctggcgaacc cggccttgag tgtggcatcg
      541 gtcatggcat tgccgccgta cttgatgacg atggtcttgt cgaagaagcg ctgaatgaaa
      601 ggcaaggctt ccgacaggat ctgggccttg tctgcaggcg aataatgact gaccgggctg
      661 gagggttcgt ccatgtgagc gtcctttggc gagaatgtgc aattttaacc gaaatggcat
      721 tgctgcgtca tgacgcaacc cacgcagccg ggttgcaaat cagttacaat cggacatttg
      781 gttttcccgt cctgtctcgt atgcacacgc ctgccatcgg tatcgtcatg ggcagccaga
      841 gcgactggcc cgtcatgcaa cacgctgctg cgcaactgaa ggcgctacag attccgtttg
      901 aggcgcgcgt ggtctcggcc caccgcaccc ccgatctgct gttcgaatat gccgaaaccg
      961 ccgctgcgcg cggcttgcag gccatcatcg ccggtgcggg cggtgccgcc cacctgccgg
     1021 gcatgctggc cgccaagacg ccggttccgg tgctgggcgt tcccgtgcca tcgaaatacc
     1081 tcaagggcat cgactcgctg ctgtccatcg tgcagatgcc caaaggcatt ccggtggcca
     1141 cctttgccat cggcgaggcg ggggcggcca atgccgcact gtttgccgca gcgatgctcg
     1201 cctgcaacga cgaaacgctg gcggcacgac tggcgcaatt gcgcgcaacc cagacacaca
     1261 gcgtgctcga gatgagcctg cccgatgcag aaccctgacc cccacaccat gatcctgccc
     1321 ggcgcgacgc tgggcatgct gggcggcggc cagctcggcc gcatgttcac cctgtccgcg
     1381 cgcaccatgg gatatcgggt catcgtgctc gaccccgatc cggacagccc ggccggccag
     1441 gttgccgacc gccatctgct ggcgccctac ctcgaccggc agtcctggca aacgctcgaa
     1501 agcgagtgtg cggcgattac caccgaattc gaaaacattc cggtcgaact gctgcagcac
     1561 ctggccaaac ggctaccggt gcgtccggga gcccacacgg tcgcaatcgc acagaaccgc
     1621 gaaacggaaa aatcctggct gcgcgccaac ggattcgaga cagctccgtt cgccattgtc
     1681 agcgagacgg acgggctgga ggcggcgtgc cgggcaaccg gctttcctgc cgtactcaag
     1741 gtggcgcgct ttggctatga tggcaagggt caggttcgcg tggccaacct gaacgaagct
     1801 cgtgcagcct tcgccgaact gggcagccag acctgcgtac tggaacagct gctcgatctg
     1861 cgcctcgaac tgtcagtagt actcggtcgc aacgccgcag gccagaacgc ctgttttccg
     1921 gttgcggaaa atatccaccg cgatggcata ctcgatacca gcatcgctcc ggcgcgcgtc
     1981 gatcccgccc ttgccgcccg ggcacgcgca gcagcgttgc atatcgcgca gcagctcgat
     2041 tattgcggcg tactcggtgt ggaattcttc gtcactaccg atgaccgcct gctggtcaac
     2101 gaactcgcgc cgcgtccgca caacagcggt cacttcaccc tcgacgcctg tctgaccagc
     2161 cagttcgaac tccaggtgcg cgcactatgc aacctgccgc tcggagccac ggtgcagcac
     2221 agcgccgcgg tcatgatcaa cctgcttggc gacgcctggg gcggcagcac gcccgactgg
     2281 ttgccgctgg tacggcatcc cgaagccaag ctgcatctgt acggcaaacg cgaagcccgg
     2341 ccgggtcgca agatggggca ctacacctgt ctggccgaaa cggtcgacgg cgcgctggca
     2401 ttggccgaag ccattcgtgc cggcttgcgt ccacgcggct aaccccggag tgctgacatg
     2461 ctgtattcca acgatttcgt ccattggttc cgctcggcgg caccctatat ccatgcgttc
     2521 cgcggcaaga cgttcgtgct ggcgttcggt ggcgaaatgc tcgccgacgg ccagtttacc
     2581 cggctggccc acgacctcaa cctgctcaac agcctgggag tgcggctggt gctggcccac
     2641 ggcgtgcggc cccaggtcga agcacacctg atcgaacgca aggcacagat ccgctacgcg
     2701 gagggactac gcgtcaccga cgatgacgca cttgcctgcg tcaaggaagc cgtaggcgaa
     2761 gttcgcgtcg aaatcgaggc cctgctctcg gtcagcctgc tcaacacccc catggccaat
     2821 gccgacatcc gcgtcgcctc gggcaacttc gtcacggcac gcccgcttgg cgtgcgcaac
     2881 ggcgtcgatc tgctgcacac cggcgcggtg cgcaagatca atgcagcggc cctgcgccag
     2941 cggctcgaag acaatgacat cgtactgctt tcgccgctcg gctattcgcc caccggtgaa
     3001 atcttcaacc tgactctgga agacgtcgcc acagccgcag ccgtagcgct gcgcgccgac
     3061 aagctgatct tcctcatgga aacggctggc gtgaccggta ccgacggtca cctgctcaca
     3121 caactcaccg taccggaagc gcaacactac ctgaccgaac gtggcaagca cgacgatgac
     3181 atcggttatt acctgcccaa tgccattgca gcctgccagc agggcgtggc gcgtgcccac
     3241 ctcatcagcc gccatctcga cggcgggctg ctcggcgaac tgttcaccca cgaaggcgtc
     3301 ggcaccatgc tcacgcgcgg ccggctggaa acgctgcggc cagcgcaaat ggacgatgtg
     3361 ggccgcatcc tgcaactgat ccggccgctc gaagaaggtg gtgcgctggt gcagcgttcg
     3421 cgcgaacggc tcgaaatgga tatcgaacat ttccaggtgc tcgaatacga tgggcgtatc
     3481 atcggctgcg cagcactgta tccattttcc ggtgatcagg ccggcgaact ggcctgcctg
     3541 gtcgtgcatc cggaataccg cggtcaccac cacggcgaca cactgctcaa gtccattgaa
     3601 cgtgccgcgc gcgcgcagca cctgcaacgg ctgtatgcgc tcaccacgca taccgagcac
     3661 tggttcatcg agcacggctt cacccccgcc cgggtcgaag acctgccggc cgagaaacgt
     3721 gcccgctata acagtgagcg ccgctccaag gtgtttgtca aaacgctgta gccctgtatc
     3781 gagccagcgc ctggatgcaa cgcccaggtg acttgccagg cggcgcgatt cagcgcacaa
     3841 tccgtattca gcattttgtc agaataatcg gcccccagac attcaccctg acgttcgggt
     3901 gacgcgcacg tcatgatttt caactttccc tctcgcctgt tgccgcgcct tgtacaactg
     3961 ctgcgtctgg ccgactggcc cgggctgccg gcaacacgat tccgccggcc aggggggtca
     4021 ggctggcgtc agcgcggcaa ggccactgcg ccgcccaacc gctacgaaac cctgatccgg
     4081 aaaatcagcg acggcatcca ccttgtggat gccgccggcc atatcgtcga actcagcgac
     4141 tcattctgca ccatgcttgg atacacgcgc gacgaaatga tcggcatgca catcagcgcg
     4201 tgggaagctc gctacagcga aacggaaatc atggccatgc tgccacgcct gcttgcagat
     4261 gataccgagc tgcgggtgtt tgaaaccgtg caccggcgca aggatggcag caccattccg
     4321 gtcgagctca acaatcgcgc agtcacgctc gatggtcagc gcttcatcct caattcggcg
     4381 cgcgacatca gcaaccggct gcgggtgcgc caggcgctgc aggacagcga aaaccgctac
     4441 cggcaattct tcgagaacaa tgtgtcgatc aaactggtgg tcgacatggc cgacggcaac
     4501 atcgtcgatg ccaacagctc tgcggcccgg ttctacggct atccgaaacg gcgcctgctt
     4561 gccatgcgca tcgaagacat cgaatgcctg acagcggaac aactcgaacg cgagcaggcc
     4621 ctggcccacg ctgaacaacg cgattacatc cagtcgcgtc accgcctggc gtcgggagat
     4681 atccgcgatg tggagatcta ttcgggccgt accgaactgc acggcagaac gctgcgcttc
     4741 tgcatcgtac acgatgtcac cgagcgccgc cgcatcgaga cccaactgca attggctgcc
     4801 agcgtgttcg agaacagtta cgaagggatc ctggttacca cgcctgacaa ccgcgtcatc
     4861 aacatcaacc cagccttttc ccgtatcaca ggttacagcc tggacgaaat catcggccag
     4921 aatccgcgca ttctgtcctc cgaactccag gacagcgagt tttacgcgct catgtggaaa
     4981 gccgttgccg agcatggatt ctggcgcggt gaaatctgga accgacgcaa gaatggcgag
     5041 gtgtacgcgg aaatgctgtc catttccacc attcgcaacg cggatggcgc cgtgcagtat
     5101 tacgtcggcg tgttttccga tatcagcgcc tacaagaaac tggaggcaga actcgaacac
     5161 atcgcgcact atgatccgct gaccggagta cccaaccgcc gcctgctggc cgaccgcatg
     5221 caccaggcca tatcacgcag ccagcgttac cagcaaccgc tggcggtgtg ctatctggat
     5281 ctggatggat tcaagccggt caacgaccgt tacggacaca atgccggcga ccgcctgctg
     5341 atcgaaatca cccaccggct cgacaccctg ctgcgcgcca acgacaccat cgcccgactc
     5401 ggcggagatg aattcgtgtt gctgctgagc gatctcgcgc acgcggaaga aatccacatc
     5461 atcatggagc gcgtgctcga tgcggtcaac cagccgatga tcatcgacgg caatacggtc
     5521 agtgtgtccg caagcatcgg cgtaagtctt tcgccaccgg acgaagccga cgccgacatc
     5581 ctgctgcgcc aagccgacca ggccatgtac cgcgccaagg aatgctgcaa gaacagctac
     5641 cacctccatg acccggaaca ggaccggcag atcaggcgcc atcacgacaa gctgcgccgc
     5701 atcacccagg cactggaaca gaatgagctg gcgctgttct accagcccaa gaccgacatc
     5761 atcagccggg aaatcatcgg catggaagcg ctgatccgct ggcatcaccc ggaactcggt
     5821 ctgctgctgc ctgcggcttt cctcaatgac attcatggca cccgactgga aatcaccatc
     5881 agccagtggg tcatcgaaca ggccctgcgc cagatgtcgg cctggcagca tcagggccac
     5941 cagatttccg tcagcatcaa catcggagcc actcacctgc tgcatgccga tttcatgcag
     6001 cacattgttc aggcactcgc gcgctacccc gaggttgcgc cggcacaact cgaactggag
     6061 atcctggaaa cgactgccct cagcgacctg caacaggcca gccacgtcct cgacaactgc
     6121 agcgcactcg gggtgcgttt cgccctggac gattttggca ccggctattc ctcgctggcg
     6181 tatttccgca ggctgaaagt cgacaccatc aaaatcgatc gcagtttcgt gctcaacatg
     6241 ctggacagca ccaatgccct ggatatcgtg gaaaacgtgg tcaggctggc gcacggcttc
     6301 aaccgcatgg ttgtggccga aggcatcgag accatcgaac acggcgcgtt gctggtactg
     6361 acgggctgcc gcatctgcca gggattcggt atcgcccatc ccatgccagc cgatgaagtc
     6421 gttggctgga gccgtcagtg gaatgcgccc gcaactggcg atgccatgcc ccccggtttg
     6481 cgtccgcagg acatcccgct gattctggcc gcgcaaagcc accgcaaatg ggtcaaccgg
     6541 ctcatcgaac agctcatcaa cccggatctg gacgatgcca ccgaactgaa cgataccgat
     6601 tgcgcgttcg gtcgctggta ccgtagcgaa ggcctgtcgc gatacgccaa cctggacgag
     6661 ttccgtgaca tcgggccatt gcaccagcac atccaccggc tggcgcgcga actggtgccg
     6721 ctgtcccggg cagggcacgc acgactggta caggagcgca ttcccgagat tcaacaggta
     6781 cacacccaac tgctgatctg ttttgccgcg ctggtcaagc gcctgtcgcc ggtcaggccg
     6841 gcattattgc cgccgcgccc ccaatgaaac aggtacaatc tgtggtcacc gcatccgacg
     6901 aaagggaatg accatgacgc aacccggaca ggaaccccgt ctgcgcgcca gtcgcaccgt
     6961 cgaacggctg gtggccggta cggaaacatc cgacggcgcc ggcgtacggc tgacccgcgt
     7021 actgactcaa ccgctacagc ggcggcttga cccgttcctc atgctcgatg cctttggcag
     7081 cgacgacccg gacgaataca tcgccggctt ccccgaccat ccacatcgcg ggttcgaaac
     7141 catcacctac atgatcaacg gacgcatgca gcaccgtgac agcgcaggcc atgagggcgt
     7201 gctcgaaaac ggcgatgtac aatggatgac cgccggccgc ggcatcatcc actccgaaat
     7261 tccccagcag gaacatggcc tcatggaagg atttcaactt tggctcaatc tcgccgccaa
     7321 agacaagtcg tgcccgccat ggtacggtga cctgcgcaga acggaactgc cgcggctaac
     7381 gacagaacac ggcgtggaag ttaccgtaat cgccggcagc tgccgcggta tcgatgccct
     7441 ggtacaacgc gaacgcaccg acccgctgta cctcgacatc cacctgcccg ccggcagcag
     7501 tttcagccag ccgctgccgc cggagaacaa tgcattcgtc tacacctatc gcggcagcgt
     7561 cagcatcggc gacaccgcgg taccgataca gcgcatgggc atcctggcca acgaccccgc
     7621 agctgacggc gtcacgatca cggcgcatga ggacgcgcat gtgctgctga tcgccggccg
     7681 gccactgggc gagccgatcg tgcaatacgg tccgttcgtc atgaacagcg aagacgaaat
     7741 tcgccagacg ctgcaggaat accgtgacgg gcgcctcggc cagcagtaaa ccgccagagc
     7801 gccgcagaac aggatgcgaa cgatgggggg ccgtcgcgca gatgaccggt catcgccacg
     7861 ccgggtaacg cgttgcctga ctgaccagac cattccggcc cccgcccgcg ttgcctgatg
     7921 gagaacatgc cgtgcctgcc acccgcctgg atcccgatac tgcactgatc ctgatcgacc
     7981 tgcagcgagg catcgttgcg ctgcccgtcg tgcacccggt tgccgggatc atcgcgcagg
     8041 caaacaagct ggccgcggct ttccgccgtc accagttgcc ggtgatactg gtcaacatca
     8101 ccggtactgc gcccggccgc accgaaacac cgaaaatgaa ggacatcacg cccgaatggt
     8161 ccgaactggt gaccgacctg gtgcgcggag cgcacgacta cgcaatcagc aaacagacct
     8221 ggggggcgtt caccggcacc ggcctcgaga ccttgctgcg tcgacttggc atcagccaac
     8281 tggtcatcgc cggcatcgcc accagcatcg gtgtcgaatc gaccgcccgc tgcgcccacg
     8341 aactcggatt caacgtgacg ctggcagtcg atgccatgac cgaccgttcc ctggaagctc
     8401 acgaaaacag catcggacgc atttttccgc gtctgggcga gactggaact tcatccgaaa
     8461 tcatcggtct gctcgacagc cgctaacgtg tcagaccgct gcggaaaccg cctgcactgc
     8521 tccctgactg caatgcagtc ctgtgcggcg ggaccgggca gcccaccagc cggcgcataa
     8581 ccggccatca gacatgaaaa acactttccg ctcactgaaa cagtacaatt accgtacctg
     8641 ggccatcggc gcactgattt cgaacatcgg cacctggatg cagcgcaccg cacaggactg
     8701 gctggtgctg accgagctga cgcaccacaa tgccaccgcc gtcggcatca tcatgtcctt
     8761 gcaattcgga ccgcagattt tgctgttgcc ggtaacagga ctggcagccg accactttga
     8821 ccgccgcaaa ctgctgtttg ttacccagac ggcttccggc ctgctggcgc tggcgctcgg
     8881 ggtactgacc ctcaccggct cggtccggct cggtcatgtc tacctgttgg ccttgctgct
     8941 cggttgcgtc tcctcattcg acactccggc acggcaggcg ttcgtgtccg acctggtagg
     9001 cgaggcggat ctgccgaatg ccgtcgcgct caactcgact tcattcaatg cagccagcat
     9061 gcttgggcca gccgtggccg gattcatcat tcacgtcttc ggcgttggct gggcctttct
     9121 gatcaacgcc gcatccttcc tcgcggtgct ggcctcgctg ctgatgctgc gccaggagac
     9181 gctgcacgct tccgcccgtc cgccacggac cggcgggcaa ctgctcgaag gtttccgcta
     9241 tgtcgccaga cgccctgatc tcaaactggc cctgctgatg atgttcctca tcagcacctt
     9301 tggcatcaat ttcccgattt tcatcgccac catggatgtc gccgcatttc atgccggtgc
     9361 ccgcgaatac ggcgtgctga cttcggtcat ggccatcggt tcggtcatcg gcgccctgct
     9421 gtcggccagc cgcacccgac tgagtctcaa tatcctgctc attgccgcgc tcggtttcgg
     9481 cgcgaccatg gctctggccg ccatcatgcc caacctgacc ggtttcggct tcgcactgat
     9541 cggcgtcggc atcgcgacac aaaccttcac cacatcgacc aacagtctgg tgcaactgtc
     9601 cagcgagcct gccatgcgcg gtcgcgtcgt cgccatcctg ttcgccatca ccatgggctg
     9661 cactccgctc ggagcgcctc tggtcggctg ggttgccgac cagtacggcc cacgcatcgc
     9721 cctggctgtc ggcgccacct cggggttgct cgcagcattt gctgcctgga tctacctgtc
     9781 cggtcgcaac agcacgatgt cacgctgacc acattccacc gctgcccccc ccgacgcttc
     9841 agcctgcaag ctgcaacgcg gacagaatgc cctcgtcaat ccggttctgc gcctgctcta
     9901 cccggctgca atcgaggggg ctgctttggc tcatgcaggc agcaaaggct gcatggtatt
     9961 cgttgtgcca atggtgcaga tcctcatcct cgccgatacc aatcttgagc aaggccgcgt
    10021 gaatggggca agcccgcgga tcagccagcg tctgcaggtc gatcaactgc ggatgctggc
    10081 gaatcacccg gatgacgctg gtatgatgga caaactgcat cgcatacagg cccagcagac
    10141 tgtctggcca gtcatgtcgc ggcagccgca gcgggtgcct gaaatgctcg accagttcgg
    10201 caaacggcat cggctgggcg accacatatc cctgcacgta gttcacgccg atcatgcgca
    10261 ccgcatcgag aatctcaacg gtctccaccc cttcgacgat caatgcgatg cccagccctg
    10321 tcgccagttc ctggatcgaa cggatgaaat gcatgtcctg cggccgttcg cgaagtgtgc
    10381 gtacaaaaga ctgatcaagc ttgattttgt cgatcggcaa ttcctggatg cgctgcaacg
    10441 aggaatacgc aatgccgaca tcgtcgaggg ccaggtgtac cccctgatcc cgcagcgcaa
    10501 gcaggtggcg caacgccatc tcctgttcct ggaaattgct gccttccatg atttcgagcg
    10561 tgatgcgcgc aggatcgatc ggcccctgac tgatcttgtc gagcaggcag gcgacgcatt
    10621 cttcggacac cgagtgcgga tgcaggttga ccgacagcca aagcgactgt ccgtaccggt
    10681 cgagctcggc caggtcggcc agcgcatggt cgaagaccat gcaggtcagc ctgaggaaat
    10741 ccgccgactg cagttgtggc aagaaatctc ccgggtgcca cagccggcct gcctcgtccc
    10801 gcaatcgcgc cagcgcctcg atgccgacca cctcgccacg tatgctgtcc atgaccggct
    10861 gataccagac ttccacgcca tgctgttcca gcatgcgttg cgccgggctc tggcgcaacc
    10921 tgaccggatc gccatgcagc acccagtaac gcgaacgcgt tcgtttatgt tccttgctgg
    10981 catacaacga ctggtcggca taacgcagca gcacatcggc attgcgggct tcgtgcagcg
    11041 gatacaatcc cacgccggca ctcagcccga catgcaccgt tacgccatca tttagcgcga
    11101 tcggcaggca gactgtctgt tcgagcttgg cgaaaatggg ctcgaactcc tcgacgcggg
    11161 tcgacccccc gagcaacagg acgaattcat cgccccccag ccgtgccacg aaatcggaac
    11221 gccgcaatgt ctgtttgagg cgctgggcga tgatgcgcaa cacctcgtcg cccgcatcat
    11281 ggccgtaagt gtcgttgatc ggcttgaaat tgtcgaggtc gagcatcatc accgcaagca
    11341 gccacccctg acgcgtggcg atagccatgg actgctcgat ggctacatcg agcgaacgcc
    11401 ggttgggcag gccggtcagc atgtcgtgca tggcttgatg gcccagcttt tcctgcgcgt
    11461 cgtgcaaatc cgaggtatcg acgatgctcc acagcacccc ggggttgcca tcagacaggt
    11521 tgatcttggc gccaagaatc ttcgcccaca gcgtggtgcc gtcctgacgg cgaaactggt
    11581 attcggtctg caccgttccg gtcgaatgca gcgcttcgtt gaacttgccc tgaaaattga
    11641 ggtaggtggc atgactgaag tggatgcaca ccacagattt ccccagcaag tcggcaggcg
    11701 aaaacttgag catggcgcac agggccgggt tgacatccat gatcatgcgc tcgcgatcga
    11761 cgatcaggat tcccgcggca ttgtgctgaa acagtgcgtg ctgataattc cgcgcccgga
    11821 tcagttcctg ctgcaacaac agctggtgcc gcttggcatc catgcgttca aggccgttgg
    11881 acaggtcttc tgccaggttg accagtatat tgcacgcgga aggaagcagc ggcacggcat
    11941 cggaacacag cagccacagc acggcccagg gttcacccgc gcgcagtacc ggcaccaccg
    12001 tacgggtgcg gatatccagc ctgttcatca atgactgcag accggggggg aattcggcaa
    12061 gctcgaccgg gcaatcgaag gatggctgca ctggcccggg cagcggcggg aacacgccca
    12121 gcccggaatg ggccagcgag cggacgaact ccaccacccg ctcatcctgg ccactgacgg
    12181 tcagcaggtt ccctggctgg ttgttcgcat tgatgcgcac aaagcagccc agttgcacgc
    12241 cggtataggc cgaggcaatc aggtcgctga taccctgcag cagtgacgct tcatcataag
    12301 tgcgattcac caggcgattc acctgggcca gcaacacgct cagcgtgagc aattgccctg
    12361 cctcgttgcg catgtctccc gaatcgattg cgatatccgt atgttggaaa gtggttgctg
    12421 tcatcttgcc tggggcatgt ggccacccgc cgccgtagcg tgtcctgccc tgcggccgac
    12481 caccaacgga acgcggcagg tccggaattg gtaacaagac tatactcaaa ttcgcggcgt
    12541 aacgccacac cgaccggagt cgatgccggc cggcccgcgc cgcacccgcg cggtatatgg
    12601 gctaccccgg tttttgcttg tcttccagac cgacgatatg cgtcaggtag tgattcagat
    12661 tgcgttcggg atgatacgga taatgttcag ccaacgtatc cagggtctgc acccgatcaa
    12721 tgcgaaaatt gcggtaatcg tcgcgatgtt cacaccaggc gagcagcgtc cagtgatcgc
    12781 cccagcccac catgcccagc ggccacagca ctcgcaccga ttcacgctgc tgcatgtcca
    12841 gatagtgcac gctcactttc tgccggtttt cgcaggctcg gcgcaactgt cggtgcacga
    12901 cgctatcggg acgaacaaag gccggaatac ggtacggcag ccgtgatgcg ccattcagca
    12961 tcggtggttc cagcatcgcc agaatcttgg cctcggccgt cttggcagcc tgcgccaggt
    13021 ccgggtccgt ccacgcctgc accatgcggc tgcctaccag caaggccgtc acttcctccg
    13081 aatcgaacat gcgcggcggc agatcgaagc cacgccgcag actgtagccc acgccggcct
    13141 cgccatcgat cggcacgccc gatatcgtca gtgcctgaat atcgcgatac accgtgcgca
    13201 ccgccacgcc aagctcctcc gacaacgatt gtgcagtcat tgccgtacgc cggccctgca
    13261 acagggtcac cagtttgaac aaccgttccg atttgcgcat gaatgtttac gatgcgccgg
    13321 acggatcccg atgatacagg gcgacttcgc tgaactggat gatgggcacg ctgtccgtgt
    13381 aattgcggat atcgccctgc aattcctcgg catgcggcat gaatgccgcc atgaaatcgt
    13441 ccaccgaatc aaacaggtaa tggcacatcg ccacaaacgg cggtgccgag cccgcttcaa
    13501 ccccgttcag tccgcgctcg accgacacgc cacgatatcc tttgcctgcg ctcagcagac
    13561 ggatggatac cggcatatgg gaaccgaggt aataccccat gtcgaactgc gtgccaccgg
    13621 gatacaaaat gctgattttg accatgtctc atccttgcgc ctgcagcatg gagtgcgccc
    13681 gactgcgatg gctgcaataa ctgtccgggt cgataaatgg tatcaccgca tcctccatgt
    13741 cctcgcggaa ccgctcctcg gcggcatgcg ctgcttcggc attgccccag cagatgatga
    13801 gtatccattt tccgtccgcg gtttcggaca ggctgcgaaa gcggaatccc ggctgacggc
    13861 tactccagtc atcgagctgt tggttcagtt ccagccagac ggttcgtggt ttgtgttcgt
    13921 acaagcggaa ttgcatcgtg accaatattg ctgacatgcg gtttccttcc cgagaaccgg
    13981 acatccggtt gatacaggtt acgtgacggc tactgacaac ggtgtgtcag caatgttcgc
    14041 accttccgtt accgcttgcg acccgtcgcg tacgctgctt tccgtaaccg catggcaatg
    14101 gcacgccact gccgcgacat gccgatgaat ggattccccg agaatacatg tggaacatgt
    14161 caatcttccg ctgtccgccg ccggcggtgc gcagcatggg cgtccctcct ggaatccacc
    14221 ccgcccggtc ttgtatctgg catgccctcc gccggcatct gcctgacgga cgccaccgac
    14281 aaaagccgcc atgcatgaca acacctgcca tcaatatctt tgttcaatat cgaaactgga
    14341 ttatgatgcc gccagtccat catggcagat ccggatgtgc gcggtcagaa cagatccgca
    14401 tcgccacacc ggatggacgg tacagaaccc gcaaggtgag gccaaccgct caaaatcctg
    14461 tatccgccat ccgcttgtca gcgcgatggc ttctgacgcg cggcatgagg agcaataatg
    14521 caacccgaac agattgtcag acaggccgtt accgccggca ccctgttgct catgacaccc
    14581 ctggccctgg ccggcccgcc attcatcacg gatgacccgg aacccgtcga tccgggtcac
    14641 tgggaagtct atgcgttttc agcaggcgcc gtcgaccacc aggcagcaac gggtttcggt
    14701 cctgcactcg aagtcaatta tggcgcggcg cctgacatcc agttacatgt catcgtgccg
    14761 attgcgtatg gcacggctac cgggactccc acccgggcgg gccttggcga caccgaactg
    14821 ggtgtcaaat accgcttcgt caactccgga gaaaacgatt ggtggcctga aatgggcacc
    14881 ttccctctgc tcgaaattcc gaccggcaat gccgggctcg ggttgggtgc gggctatgcg
    14941 cagactttcc tgcccatatg gctgcagaag aatatcggta aatggacaac ttatggcgga
    15001 ggcggctact ggaccaaccc aggccccggc aatcggaatt tctggttcac cggatggctg
    15061 cttcagtatc aggtgacgga tgcactcgca ctgggcgcag aattgtttca ccagacatcc
    15121 agcatgattg ggcgcgctgc aagccgcggt ttcaacctgg gcggcatcta cgacctgaat
    15181 gaacaccatc acctgctgtt ttctgccggg cgtggtggca tgctctatgc cgtcaatgcg
    15241 gccagcgtga tcaatccatt cacctattat ctcggcttcc aatggacatt ctgacgcatg
    15301 tccctgacgg cagaaacagt gctcattcca gcgaagcagc gatgaccatc atgggcaggg
    15361 tttcattcgc acgatgccag gtgccatacg aaatggttga cgcatgcctg cttcagctta
    15421 gccacgcccg gagaacgcaa gctcaacgtc gggcaggttg gcaagcacct ggtcataggt
    15481 atcgtaacgt tccagcacct gaacaaccgc tgaaagtggc tcagcccaga tttcgggcag
    15541 atcacgttct tgtggttccc tgattttgaa ggcgagactc tctatgggcg gatgaaaccg
    15601 tgcgtttaca ccaggcctgt ttcctctggc atcgacccgg taccaaccga aggtttcgag
    15661 ataaacagca ttcaggccgt gcaaacagta tggtgctcca ccatcgccga cagatagtcg
    15721 ctgatagcac aggcccgccg gaatgccgtt ggcccggagt agtgcggcca gtagatggct
    15781 cttcgcatag caatagccgg tcttgtgacg caagacatcc gacgccttgc aggtcacggg
    15841 attgagcttg tgatccaaac tgtgatgaat ttcgtctctg acgaactcaa agcacatgcg
    15901 cacgagttcc agctcggaac tggcgttcgc tgccatgctc atggcggcag acccgacttc
    15961 aggcacatcg aagtcaatat acgtgctgga agaaaggtat ctctgcattt caggctccgc
    16021 tatggcgtga gtggatatct gaggaaccgc aggtctattc ccgcaaagct gcgacagcgg
    16081 catgacgtga tgcaaatcag ggggctatta cagaaaagaa aaagcccagc ataagccagg
    16141 ctttatttga aatattttac acttttcctc tcaggtaaac ctctagaacg gaatatcgtc
    16201 atccatgtca tcaaaaccac ccgaaggttt gcccgcaggc gcgccctgac tggaagcggc
    16261 tgccggacga ctgttgcgcg cgggtgcttc gcccggcatg tcatcatcca tgcgcgccgc
    16321 gccgccacca gaacgtccgc caagcatttg catcttgtcg gcaacgattt cggtggtgta
    16381 ctgatcctgg ccttccttgt tggtccactt gcgcgtgcgc agactgcctt caaagtagac
    16441 gggcgagcct ttcttcaggt attcgcccgc aacttcggcc agtttgccga agatcaccac
    16501 gcggtgccat tcggtacgtt cctgcttttc gccgctcttg tccttccagt tttccgtggt
    16561 ggccagcgtc aggctgacca tcgcgtcacc actcggcatg tagcgggttt ccggatcgcg
    16621 gccaaggttg ccgatcagga tgactttatt gaccgatgcc atgcgtatat ccttccagta
    16681 atttctgtgc ggaggcctca tcccagccgc gttgcgccac cttgaggatg gcaacctgct
    16741 cgccggcaat cacaccggcc tcgaacacgc cgtcgatcgc ctgcagcccg gcggccagtg
    16801 cccgggcctg cgcatcgtcg atggcgccaa gatggaacat gcgcgtcttg acggccggcg
    16861 gggctttcat gccaatcgca gccaatagcc acaacacgat gatgccggca gcaaattcat
    16921 acactgcgga atacccatag tgctgtgaca gccagccgcc cattgcgcca ccgaaaaaca
    16981 gaccgatgga ttgtgaggta ttgtacaccc ccatcgcggt ccctttggcg cctgtcggcg
    17041 cgatttttga gatcagcgag ggcagcgtcg cttcgaggat gttgaaggcg atgaaataag
    17101 ccgtcagcgc ggccacaatg ccccagaacg actgcatgct ggcagccatg ccgatctgcg
    17161 cggccagcag cagggcaatg gccgccacga acacctgttt gagtttcgcg cgtttctcgc
    17221 catagatgat cgccggcacc atgaggatga aggccgccac ga
//
LOCUS       DAIANL010000081         7280 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3167496, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000081 DAIANL010000000
VERSION     DAIANL010000081.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7280)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3167496"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1543 a         1952 c         2326 g         1459 t
ORIGIN      
        1 tccggcccgg aatcagttcc tgcgcataga ccaccatggc cgggaatgcc gaggccagca
       61 tgaagccgct gatcatcgac agcgcgagtg agccagacag gccggcatag ggcagtgcca
      121 gcgtgaatgg ggcgatgccg agaatcgaga tccagatcac gcggcgtcgg ccaatgcggt
      181 caccgatcgg gccgccgagc agggtgccga gcgcgaccga ggccagaaag ccgaacaggt
      241 ggtactgtgc ggtctgactg gtgatgccga actggtgcat cagaaagaac atcaggtagt
      301 tgttgatgct ggccaggtaa aagaacttgg aaaacatcaa catgaccagt acgaccagcg
      361 tgatgaccgt ctcgcgccgg ctgagtggcg tttgtgtcgc agtggcggat ctgcggctct
      421 ggcggtgctg ctggcgatac caacgaccga cgacggtaag aatcagcatg ccggccagcg
      481 cggcaagtga tacccaggcg atgctgccct ggccatgcgg caggaccagc caggcggcga
      541 ccagtggccc ggctgcggct cccgcattgc cgccgacctg gaagatcgat tgagcgagcc
      601 cgtgctgtcc ccctgatgcc atgcgcgcta cacgggagga ttctgggtgg aaaatggccg
      661 agcccatgcc gaccagagcg gccgccgcca gcacggcagg aaaatcgtgt gccatcgaca
      721 gcgtcagcag accggcaaag gtactggcca tgcctgcagg cagcgaccag ggcatcggac
      781 gacggtcggt gacgtagccg accagcggtt gcagcatcga ggcggtgagc tggaatgtca
      841 tggtgatgag gccgacctgg acgaaactca gactgaattc atgtttgaag agcggataga
      901 gtgccagcag cagcgactgc atgacatcat tgaggaaatg cgacaggctg atggccgaca
      961 gaataccgaa cgccgtgcgc gcctgggccg tgtgcggcgg ctggagcgcg gcatccccgg
     1021 cgagcccggt ggaatgtgcc attgactggt tgcttgcaga actgttcatg gaaaaattat
     1081 aacgcaaggt accaatgggt gccaacggaa catttgtgtc ggtgaaggtt ataatcaggc
     1141 atcgaattct gtggcggacg cgacatgacg acagcgacga acgggcggcg cgccaggctg
     1201 gtgcgggcgc tgctgtgtgg cctgttgctc tggacctgcg gcgttgctcc ggcgcgggca
     1261 ctgccgtcca cgttcaacag caccatcggc gatgccttgt tgtgcctcga tgcactcgat
     1321 ccgggttatt tctataccta tctgagccag gaattcggcc agccatacca gcaccagggc
     1381 ggggcgtggt ggttcaggac gccgaaaacg aaattgtgga acgtgccaat cacctcgatc
     1441 ctggtcagcg atggcaacag cagcctgttc ttcattgccg ccattaccga tctgtccact
     1501 gacaagctcg cacgggccat ttcggacaac gctggcattc tgttcgccac gggcgatggc
     1561 agcgcctggc cggtgctggt atccaatact ggcagcatga tcgcctgggc gggcaatggt
     1621 tccaagatct actgcgccaa ggaccgccga ctgttcaggc agtagccccg ggcagaggcg
     1681 cgtgctgccg atacaacata tggcttgctt tggtgctatg tccgacctat catctgtccg
     1741 gacggcgaca tacatacatc agcaatgcgc agcgtcctgc tggtagcggt acccagcggg
     1801 gcgcatcctg gttgtgtcat gttcaggttt tgatgcgagc gcccatggac caagccacac
     1861 ccaccgttcc tgattcgtca gccagtctct ggtacgcgca agccatggag caattggtcg
     1921 gcgtgatcca gcaactgtcg cttgcacgcg atctggatac ggtgatgaga atcgtgcgcc
     1981 atgccgcgcg ccagctggcg caggccgacg gggcgacatt cgtgctgcgc gatggcgaca
     2041 gatgtttcta tgcggatgaa gatgccattt cgccgctgtg gaaggggcag cgcttcccca
     2101 tgaacatttg catcagcggt tgggccatgc tcaaccgcca gccagcggtg atcgaggata
     2161 tttacgccga cgcccgcatt cctgctgatg cctaccggcc caccttcgtc aaaagcctgg
     2221 ccatggtgcc gattcgcact gtggatccgg tcggggccat cggggtctac tgggccacgc
     2281 ggcatcagcc ggccacggaa gaggttcggg tcatccaggc gctggccgac atcaccgcag
     2341 tgaccatgga gaacgtgcgc gtctatgccg agctggaaga gcgcgtccgt cagcgcactt
     2401 ccgaattgca ggccatccta gacaatgtcc aggcgggcat tgttttttca gtgcaagggc
     2461 tgatcgtgcg cgccaacccg aaatcggccg aaatcttcgc actgcccgac gctgcgagcc
     2521 tgctggagcg gcagacagtc gaggtgctgc acacgcccgt gcttgatcag acaaggcaac
     2581 atctggccgc aggcaaagtg ttcgacaccg agacgaaact gctgcgccac gatggtagcg
     2641 aattctgggc gcgtctggtt gccaagccca tggtgacaca ggggcaaccc ggtggggaaa
     2701 tctgggtgat ccatgacatt ggcgaggcca aggccaagga gcgcgcgctt gaggcaatgc
     2761 gtgtggccgc cgaggaagcg acgcaattca agagcgagtt tcttgccacg atgagccacg
     2821 aaatccgtac gccgctgact ggcatgctgg gcatgctgga gctgctttct ctgagtggtc
     2881 tggctcggga acagcgcgtc acgctggatg cggcatggga ttctgggcgt ggtctgctgc
     2941 gcatcgtcaa tgacattctc gactggtcaa agatcgagga aggcaagctg gcaatcttgc
     3001 ccagccccac gtcgataccc cgtttgctgc aggaatccgt gcgcatttat tcgcgcattg
     3061 cgagcgccaa ggggctgttg ctgcacgagc acagcgaccc gcgactggcg gcagcctatc
     3121 tggtcgaccc attgcgtctg tcgcagatat tgaacaattt tgtcagcaat gccatcaagt
     3181 ttacccgtca aggcgagatc gagttgcagg cggaatggct ggaacggctg caaagcggtg
     3241 acaggttgcg catatcggtc aaggacagcg gaatcggcat cccgcccgaa gtccaggcgc
     3301 agctgtttga gcgctatcat cagggcagcg cagacacggc tcgcatgtac ggtggcaccg
     3361 gccttggcct gtcgatctgc cggcggctgg ccgacatgat ggacggacag atcgcgctgg
     3421 aaagcgagcc cggcaagggg tcggtgttta gcgtgacact gatcctgcca gtgtcgtccg
     3481 acctgggaga gggcctgtcc agttcccgcc cggatgtgga gcagtacgcc gtgacgccgc
     3541 tgttttcccc ggacgaatgg gcgccgcgga ttctggccgt ggacgaccat ccaatcaacc
     3601 gcgacctgct ggcgcgacag atccgtctgc tcggcctgcg tgccgagacg gcagaaaatg
     3661 gtgttgctgc actgtcgatg tggcgcaagg gcggattttc catggtgatt acggattgcc
     3721 acatgcccga gatggatggt tacgatctgg cgcgtcgcat ccgcagcgaa gaagccgcgc
     3781 aatcgcgccc gcgcacgccg gtcattgcct ggacagccaa cgcgctggtt gaagaggcgg
     3841 agcgttgcca tgccgtcggc atggacgact tgctggtcaa gccggcagat atggcccagt
     3901 tgaaacgcca gatggaaaaa tggcttgaat tgttcaagct ggagccggcg ccggtcgatt
     3961 tcgatgtgtt gggcagtatt gtgggcgatc gcccggcgca gatgcatctg ctgcgcgatt
     4021 atcgcgaaca catgcgagcg gaccaggccg cgctggcaga gatgatgcaa cgcgatgatc
     4081 gcagcgcagt cgggcactgc gcgcaccgga tgaaagggtc gagtcgcatg gttggagccg
     4141 gatcgctggc gcaggcgtgc gatgccgtcg agcaggcggc acgcgacggc aacatgaatg
     4201 ctgcgcgtga tgccatggcg ctgctggaag aagcgctggc gcgtctggag cggtttcttg
     4261 ctgaaagcgg aggcaacatt gatactgact gagctgcacg tgctggtgct ggaagatgat
     4321 gccttccagc gcaggctgat tgtcggcatc ctgcaatcga tgggggtgga gacagttctg
     4381 caggcagaga atggcaggca ggcgctcgat atcatccacc agacgccgag ccgcatcgat
     4441 gtggcgttat gtgacctcaa catgccggaa atggacggca tggaattttt gcgccatctg
     4501 ggcaaggagc atcataacat cttggtcatc ctcaccagtg cgctcgacga gaagctgctg
     4561 gcctcggtgg gtcggatgac acggatgtat ggcgtcgccc tgctgggcgt gatggcgaaa
     4621 ccggtgcgca tcgcggatct gggtgagttg ctgtgcaagc atgtgccgtc ttcgggcgtc
     4681 gtgcgccggt tgtcgccaca caatttttct cttgaggaca ttctgcaggc ggttgcagac
     4741 gatcagttcg aaccgtggtt ccagcccaag ctggacatgc gtagcgggcg gctggtaggt
     4801 gcagaagcgc tggcgcgctg gaagcatccg cggcatggcg tggtcggccc ggccgatttc
     4861 attccccggc ttgaaaccac cggtaacatt gatacgctga cgttcagcat cctggaaaaa
     4921 tctgtccggg catgccgtgc gttgctcgat gctggccatg cgatgaccat ttccgtcaac
     4981 ctgtcgccga cctcgctgga cgatacttcg ctggcggacc ggattacccg catcgtacgc
     5041 caggaaaaca tcgaacccca tcacatcatg ctggaaatca ctgaatccgc ggccatgacc
     5101 cacgtggccc cggcgttgga gaatctggcg cgtctgtgca tgaatggctt cgccctgtcg
     5161 atcgacgatt acggcacggg ctcgtccaat ctggaacaac tgacacgcat cgcctttagc
     5221 gaactcaaga tcgatcagtc attcgtgcgt gattgtgcgc agaacgaagc gcagcgtatc
     5281 gtggtcgagt cgagcatcga tattgcgcgc aaactgaatg tcaccagtgt ggctgaaggg
     5341 atcgagaccc ggcaggactg ggagacactc aggaaagccg gttgtggtgt ggcacagggg
     5401 tatcttgtgg cccgccccat ggacgggccg cgatttttca gttttgccga cagtcatgat
     5461 ccggcgggct ggctggcggc gatggtgtaa ccgcaccggg gcgggtgccg tcgttgcagt
     5521 catctccggc attaatgtaa atgtgatgta tggcggcgat ttgaaatccg ggattgaaat
     5581 cacgctttgt cactgattgc ccggaacccc tgcattttct tgccctcctg aaaccggatg
     5641 gattgatcgc tcccggcaat ggctgcatcg tccgcggtgc atttcacctc aggtcagggc
     5701 ttgccaaagt gattgtccca gatgcgcatg cttgggcatg acggtaaccc ggatgaacgg
     5761 gtggtcacga tgaatgaagc atgtctgcgg actggattag acatcatggt cgccagccgg
     5821 tagcaggtgg ccagcctcga taacgcaggt tgcaccacga aggtgccgag ctgcatactg
     5881 tacttgttaa gttcacagta aaagagaggc tgaccatgaa agaggatacc aaattttttg
     5941 tagggcttga cgttcataaa gattcaattt caattgccgt ggccgaatcc gggcgtacgg
     6001 cagcaagatt gatcggacag attccacatg atgtggagaa attgcgtacc aaattgcgtc
     6061 agatcggtga gcagaccagc tccatacaca ttgtctatga agcaggtcca acaggatatg
     6121 gcttgcagag ggcattggtg gcatacggat acggctgcga ggtgattgca ccttccctga
     6181 ttccaaaacg cgggggagac cggatcaaga atgacaagcg tgacagttta cgactggcag
     6241 agctttcccg tgccggagag ctgaccagca tctggatacc aagcgccgtt gatgaggcgg
     6301 ttcgagattt ggcaagagcc agggaagatg cgatcaatgc aaggcggcag gtgcgacagc
     6361 aaatgagggg ttttctcttg cgtcatggca tacgctattc tggtaaaacc gcttgggcaa
     6421 agacatttta tcgctggttg gggacattga ctttcccaga gaaaaacagt catgccgcat
     6481 ttaccgaata ccagttggca gttgaatcgg ctgacaagcg ggtggagcgc ataacggatg
     6541 cgctgaaaga atccattcat gactggcatt ttgaatcggt tgtcagggct ttgtgcgcat
     6601 tacgggggat tgatctgatc agtgcgatca gtttggtggc tgaaatcgga gacatcaacc
     6661 ggtttgagca tccacgcaaa ttaatggggt atttgggatt ggttccatcg gaacattcca
     6721 gtggcgatcg ggtaagtcgc ggggccatta caaaatgcgg taacagccat gcgcggcgat
     6781 tgctaacgga ggctgcctgg aattaccgct ttccggccaa aataggctac caggcgcaac
     6841 ggcgacagga ggatctgaac cagaccgtgc gtgatattgc atggaaggcg caactgaggt
     6901 taagcggccg cttcaggaag ctgacgggca ggggtgttca tgcaaacaaa gtttgtgttg
     6961 ccattgcgcg tgaattggct ggttttatct gggcagttgc tcagagcgcc aatttgacag
     7021 tctgaaatga acagtctgat cgatcctggg atgtgaagtc agcaaccgaa ggagtaaccc
     7081 ttgtgcctgt tatggcggct tcccagtgaa tgacccgtgt tattagagcg aggcaggctc
     7141 cgcgacggat cgatgaaatg tggtaaccaa cccacggata tgagcatgat tcatcgtcgt
     7201 agccagactt cgcatcccag ggtcgatcag gatttattga cttttgcgac catatgagca
     7261 ggccgttgaa aaagtactgc
//
LOCUS       DAIANL010000082        28467 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1733817, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000082 DAIANL010000000
VERSION     DAIANL010000082.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 28467)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28467)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28467
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733817"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5293 a         8110 c         9488 g         5576 t
ORIGIN      
        1 cttgcggtgg agaaactgag gaacgttcgg cgattcatgg gcatgtcagg ctctgttagc
       61 gggtgggccg ggtatggctt ctgtcggtgc gggcaggtcg ggacggtatt tttccagcat
      121 cccgacaatg aacgccgacc atatggcatt gggttgccag atacttttca tcaggctgaa
      181 cgcctgttca gccggtgtgt tgcacttgat cgccagatac aggccgatgc acgtggaaac
      241 ccgcatgttg gctgcacaat ggacgaaaac cttttgatgt cgatgcgctt ccagggctgc
      301 aaaaaaggcg agcatgtccg cttcgcgcgg ggcgtcgaac accatcggaa tatggatgta
      361 gtccatgccc agggcgcgta cgcaggctgt ttcatccggc agggcgtaac gcggatccgt
      421 gtgcagggcc aggttgatca cgacagcgaa gccattgcgc gcaatttcgg ccagttctgc
      481 ttcggaaggc tggccggatg cggacaaccg gtcgtccact ttccggtagt tgtaaatgtc
      541 gccgagagtt gcatgcatcg tgacgtccgt cccaatcgct gtcgccgcac cgacagtaaa
      601 gcggaaattg tgccggatgt ctatgccact gcgtaacatt cgcacagcag ccgggcgcta
      661 tggccatgaa ttggcgccaa ctgcaaagtg ctcgaagaac gcactagcgc ggacgggacc
      721 tgcttccgcg tgagcggcga tgcggaggtg ttgaacagtc cgggcgagcg cttggggcag
      781 atggcacagt aatacgctat catccgctgg tttcaggtgt ggccatcaat ggaaaccggc
      841 ttggctcgtg tatggattcc acggtcagat cgatgtccgg catcggctaa cacaggtgat
      901 cgggcgttgg ccattcgatg cgggtgatgt gttccatggt cgcttgtctg aaccaggtaa
      961 attccgcaca gggaacaaac ggttattcgt cttgagcagc agccggaagc ctttgcccga
     1021 tgccagggaa acttcagctt cagcagtgcg tgtgccaggc atggatgatt tcatcctgat
     1081 gctcactcac ccacagggca acttcgttga tttgccgggt tgagcgccct tgattcatcg
     1141 ccagcccaat ccggggttcc agccagaatt ggcttcgccg tccgtatggg cgacatgaac
     1201 atgcatgcgc ggttcttcac gcgagaaaaa gaaaaaccgg aatgcgccat gacggaagac
     1261 ggtggaagcc atgcactgca tcatggcagt tccggattga aaatgcgcgc cagatggttt
     1321 ggacacgcgg ttgcttacca actgcccgtg ttcattggca tgatgaaggg gcttgaagca
     1381 cggttcgttc cacctgcacg ctgccattgc tgtcgccaag ccataattga ccatcctgaa
     1441 tcgtgcattg cagttgcatg tttctttgca ccagtgcggc gatggcgcgt gtgctttcct
     1501 gcgccagttg tatgacggtg aggttctttt gtcgggcaag caggctgctg ttttgtgcga
     1561 accacagctc ggccgtgcgc ccaccgtagc agaatacgac gacctgtcgg gcgcgtccac
     1621 aggctttgcg gacgagtttt tcgtcaggcg ttccgacatc gatccacagt tcgattgcac
     1681 cggtcagatc catctgccag atatcggctt cgtcctccat cgtcatgccc tgaccaaacg
     1741 tcagattgtc gctggcatgc acggcaaagg ccagcaggcg taccatcatg cgctcatcgg
     1801 tttccgaggg atggcgggcc agcgtcagcg catgatcctg atagtaatgc cgctccatgt
     1861 ctgcgatttg cagcctggct ttgaaaacgg ttgctttaat ggccatgttg tacgggattg
     1921 caggtgcccg aatgcgcata tccggcattc ggggtggcac agcgtttgtt gatgctcagg
     1981 ctttcggcgc ttcggcctgg cgcaggcgga tgtgcagttc gcgcagctgc ttttcgtcca
     2041 cgttgttcgg cgcttgcacc atcaggtcgt tggcgcgctg ggttttgggg aaggcgatga
     2101 cgtcgcggat ggattcggcg ccggccatga gcgcgaccag gcgatcgagt ccgaaggcaa
     2161 ggccgccgtg cggtggcgcg ccgaactgca gtgcgtcgag caggaagccg aacttttcgc
     2221 gcgcctgctc gctgctgatg ccgagtgcgc ggaatacgat gtcctgcact gcctgctggt
     2281 gaatgcgcac cgaaccgcca ccgatttccc atccgttgag caccatgtcg taagcgcgcg
     2341 agagtgcggc accaggattt tcgctcaaca ggtgttcatg ccccggtttt ggtgcggtga
     2401 aagggtgatg cagcgcatcc cagcggtgct cgtccgcgtt gtattcgaac atcgggaaat
     2461 cgaccaccca cagcgggcgc cagtcgcctt ccggcgtgcc caccctgagt cgtagcgcgc
     2521 cgagcgcgtc gttgacgatt ttttccttgt cggcaccaaa aaatatcagg tcgccgttgc
     2581 cggcgccggt gcggcgcagg atgtcggcca gcacgggttc gggcaggaac ttgacgatgg
     2641 gcgactgcag gccggtatcg ttgagctggc tgacatcgtt gaccttgatc caggccaggc
     2701 ctttggcgcc ataaatcttg acgaattcgg tcaggtcatc gatatccttg cgcgacagcg
     2761 atgcgccgcc gggcagtcgc agcgcggcca cacgaccgtc ggcctggtcg gctgcggcgc
     2821 ggaatacctt gaagtcgacc gatttcatca ggtcggtgag gtcggtcagt tcgagcgcca
     2881 cgcgcaggtc gggcttgtcc gatccatagc ggcgcatggc ctcgctccag ggcatgcgcg
     2941 ggaacggttt gggcagttcc acatcgagca cctctttgaa caggtcgcgg atcatgtgtt
     3001 ccaccaaatc catgatctgc tcctcgtcca tgaacgaagt ctcgatatcc acctgggtga
     3061 attcgggctg acggtcggca cgcaggtctt cgtcacggaa acatttggtg atctggaaat
     3121 agcggtcgaa gccggacacc atgagcagtt gcttgaacag ttgcggcgac tgcggcagtg
     3181 catagaactg tccggcatgc acacgtgagg gcaccaggta atcgcgcgcg ccttcgggtg
     3241 tcgagcgggt cagcatcggc gtttcgattt ccatgaaacc gttgcggtcg aggtgatcgc
     3301 gcagcgtttt cgccacgcgg tagcgcaggc gcaggttgga ttgcatggcc ggccggcgca
     3361 gatcgaggta gcgccaggtc aggcgtacgt tttccgacaa cgattcgtca tccatctgga
     3421 acggtggcgt ggcggagggg ttgagcacct cgatgtgatg cgcgagcagt tcgatctcgc
     3481 ctgtgggcag gttggcgttg tgcgtgcctt cggggcgcag ccgcacccga ccggtgattt
     3541 tcagcacaaa ttcgctgcgc acctgctcgg caacgtggaa actgtccgcg cggtcgggat
     3601 cactgacgac ctgcagcatg ccttcgcggt cgcgcagatc gatgaaaatg acgccgccat
     3661 ggtcacggcg gcggtgggcc cagccgacga cggttacgat ttggtccaga tgggcgcgtt
     3721 gcagggcgcc gcagtagtga gtgcgcatga tgaaagaatc aggatggttg ttgaatcggg
     3781 tgtgcggcgg ctgccggcgg cggggtatga actgcaccca tggtgatgat gtacttgaat
     3841 gcctcgtcta cccgtatgtc cagctcgatt gtctgtgaac gcggcacgaa gaaataaaat
     3901 ccgttgaccg ggctgggcgt ggtgggaatg aatactgcga cgtgctcctc gtcgagatgg
     3961 ccggcaatgc tgacggggag gttggtctgg aaggcaatcg accagctgtc tgcgtgcggg
     4021 tagcgcacca gcagtacctt gcggaacgcc tggcccttgc ccgacagcaa cgagtcggag
     4081 acctgcttga cgctgtaata gatcgacttc accaccggga tgcgccgcag cagcatctcc
     4141 caccattcca cgaggcgctg acccagaacg ttggtggtga tcatgccgct cagcaggatg
     4201 acggtcagca tcaggatcac gcccagaccg ggaatcctga caccgagcca tgcctccgga
     4261 cgccattgcg ccggtagcag cagcaggctt tcgtcgaggg tgccgatgat catgtccagc
     4321 gcgtacagcg tgatgcccag tggcacccac agcagcaggc cggtgatgaa gtaacgtttc
     4381 attaccgcag gttcaggaca tgcaggccgg gcaggcgcct gcgccgcaag ccggagcggg
     4441 cgattcgctg gcgggcgggt tcttgaaatc ggtggcgtag tagccgttac ccttgagctg
     4501 gaagccggct gcagtgatct gcttgctgaa ggaggacttg ccgcattgtg ggcagaccgt
     4561 cagttcggcg tcgctcattt tttgcatcac atcctgtgca aaaccacatt cgttacactt
     4621 gtatgcatag atcggcatga ttcggactcc tggtcgttcg acgattggac gctattgtac
     4681 ctgaaaccgc tcgcatgaag cagagtgcct gccacagcag ccaggaacga agcagacccg
     4741 cccgtgccgt gcaacggcat ccgcgatgca gcatcgagat gcgcacggtt tccgtggcac
     4801 ctacatgggg ccgggggcgc tgatttcaag aggtgtggca cattcaggtc gatgtcagtg
     4861 ctttacgcgc atgatgcgtt tgaccgcctg tatttcgcgg acgctcgtca cccgggcgac
     4921 acgcagattg agtgcaggcg ccgcagcagt ggcagcgcgg caaacaacgg gtggtgtggc
     4981 ggtagccaac ctgctatcct ttgccggttt tttgggttca caacaggacg ggaagacgat
     5041 gttgaaaaaa tggttgatct gggcgctgct ggccgtgctg gggttgtcgg cgtgtggcca
     5101 ggccgaggat acccaccctg gccagccggt gaccaagcgc aaggtgatat tcaagaccat
     5161 actgcgcaca ttggaaccga tggggctggt ggtgcgcggc cataacgaat ataacgcggc
     5221 gcaatttcgt gccgcggccg ccaagctgca ggagctgagc actcagccat ggcagtattt
     5281 cacgccggga agcaattatc cgcccacgcg cgccaagccg gatgtgtggg ccaagccggc
     5341 cgagttcaag caggcgcagc agaagttcat cactgcggca gcagggttga atcaggcggc
     5401 gcagagcaat aatcttgaca ccatcaggcc ggcgtttgcc gcagtggaat ccagctgcaa
     5461 gtcgtgccac cagcagttcc gtggcattcc gcactgaggc tgcgtgtacg gatcatgtgg
     5521 ctcgatctga tcctgtcgac gctggcgttc atgggggcag cctggtggct caggcgttat
     5581 tttgacgacc aggggtttcc gcccgggctg acgcgtaacg tgatggtgat ggtgattgcc
     5641 accgcagcgt cgtttgccgt gtcagcgctg gtgtcgtggg ttgatggtga gccggctgcc
     5701 gaacagcagc tcatgcagca ggcaggtcag ccattatcgc aactgccgct ggggcactga
     5761 accggtactc gtttcttttc tcacccgacg cctgtcgggt cgtgctcccg gcccggccgg
     5821 gacagtgccg cgtgtctgcc tgccgttcag cctgccagca acggcagcag ggcgaccgcc
     5881 gcagcggcga cggtgaacag cagcagcgcg cgtccattgc tggccagcgc cggacggggc
     5941 acagcggcgg agaccggctt gcgtccggtg atcatcggca gaatcaggtt gtcacgcttg
     6001 tacaggcggt agaacagcac ggccagcacg tgcagcgcga tcacgcccgc gaggacgttg
     6061 aaggcgatgc cgtgcatatc gctcaaccgg tcactcaggt cgggggtgat acggtgcgcc
     6121 agtggccccc aggttgagac ttcgtcattg gaaaacagtc cgctgacggt aaccgtcagc
     6181 gagagtgcaa gcagaaccag cactgaccaa ccacccagcg gattgtgtcc ggcatgcgtt
     6241 cctggtttac gctgcagcag tgtacgcaca taggcggcta cctgaggcgg actggtgacg
     6301 aactggctga agcgggcagt ttcactgcca aaaaaacccc acatcacgcg gaacagcagc
     6361 agggcgaggg cgcagtagcc ggcccagatg tgcagcgtca tggcgtcgtc gagcaattca
     6421 ccactgaccc agcacacagc aatcgtcccg accaggcccc agtgaaacag gcgggtgggc
     6481 agatcccaca gggtgatttc ggtggtatcg gtcatgggtg gcctttatca gtgcatgttt
     6541 gcggaattat atcgcgatcc cggcgtcgag cgcatgcagg ctcagccggt atccggtgtg
     6601 cttccacagg cgcgattcct cgccaagcgc ggccgcggta agcgggttgg cttcgagcca
     6661 ggtacgcgcc agatgcagcg cacagccctc gctgtcgaac agcagacgca gttcaggcat
     6721 gctgcggttg ctgcggctgc ggcagaacag caccgcaagt cgcaggatga tgatcatcgc
     6781 gtgggaaatg ccacctcggg ggacatcact catcttgtcg aggctgcccc gctgcatgcg
     6841 caccagcatg gcgacgcgtg cctgatcctt ttgcgaaaag cccggcatgt cagcgtgggc
     6901 gaggatgtaa gcaccgtgtt tgtggtagcc ggaatgtgcg atcgacaggc cgacttcgtg
     6961 caattgtgcc gcccattgca gatagttcag gcattcctgc gatacgctgg catctgtgcc
     7021 caactggtgc atgacttgtc ggaacagcgc cagtgccagg tcggccacgc ggcgtgcctg
     7081 gcggctgtcg acgtggtagc ggcgcgcaaa ctggctgact gtcgcgctgc gcatatcgtg
     7141 gtgatggaaa cgcccgcgca ggtcgtagag cacgccttca cgcaatgcac cgtcagccac
     7201 ggtcatgcgc tcgatgccga attcgtcgaa cagcccggtc atcaccgcca ccccgccggt
     7261 gaacactggc ttgcggtcgg acttgatgcc ggcgaggtcg agccggttga tgtggccggc
     7321 ctgcaacatg gtttcacgca ggcgggccag gccgtcgcgc gtgatgccct gttcggccca
     7381 gccgttttgt accagcacat cactgatgac gcgcgcggta ccgctcgacc cgacggcttc
     7441 ggtccatttg tcgctatagc tgatggcgat tgactgggcc tgactgcggg cgacgatttc
     7501 ggcctggccg aggttctgtc gcgtgatctg gccatcggcg aagaaccgct ggctgaaatg
     7561 cacgcagccc agggggaggc tttccatgac ctgtggtgtc atggcgtgcc cgatgacgca
     7621 ttcggttgaa ccgccgccga tatcgatgat caggcggggg tgttctgcgg ccggcagcga
     7681 atgcgccacg ccgatgtaaa tgagtctggc ctcttcgcgt ccggcgatga tttcgatcgg
     7741 gtagccgagt gtggcttcgg caagctggag gaattcttcc gaattttccg ccacgcgcaa
     7801 tgcgctggtg ccgacacagc gaaccgcagc ctggggcagg ccgcgcaggc gttcgctgaa
     7861 gcggcccagg caggccagcg cccgggtttg cgatgcgcca tccagccgtt tgtcggcgcc
     7921 gattccggcg gtcagacgta ccggttcttt cagcgcgtcg aggggataga gctggtcttc
     7981 gacgatgcgg gcaacttgca gcttgaagct gttggaaccc agatcaacgg cagcgagggt
     8041 agtgtattcg ttcatgtcat acggctggcg gtgagcatcg gcaacctcgc cgagcgaggg
     8101 ttggcaaaag ccatgctaca ttctcacatt ctgcaccgta cgtccagcgc tggcagccag
     8161 cggttgcctt tcaggcgcca tggcgcacga actgttcggg gcgaatgcca gtcaggcgga
     8221 gcagggcgaa ataaaccagt cctccgccac tcacccacag gctcaggtgg gcgagtttcc
     8281 agcgaaaacc ggcgtgcagc caggatggat catggccggt gccgaaccag agcagggcgg
     8341 ccatgagcgc ggtggcgact gctacgcgca cgatgaatcc cggccagccc ggttcggggc
     8401 gatagatgcc atgtgccagc agccgttgca gcagaatggc agcgttgatg caggctccca
     8461 ggccgatcgc cagcgccagt ccggcgtgct gcagtggacc gatgaacagc aggttcatca
     8521 gctgcgtggc ggtcagtgtg aggatcgcga tcttgacggg cgtgcggatg ttctgccgcg
     8581 catagaagcc gggggcaaga attttcacca tgatcaggcc caggagtccc agactgtagg
     8641 cgatgagcgc gcgtcgcgtc atccaggtgt cgtgtatgct gaaatggcca taggcgaaca
     8701 acgtactcac cagcggcagc gccagcgctg ccagtgcagc tgcagccggc agcgccagca
     8761 gcagggtgag gcgcaggccc cagtcgagca ggcgcgaata ttgggttgcg tcgttgtcgg
     8821 cgtaatgctg cgacaggctg ggcagtaaaa tggtgcccag cgccacgccc agcatgcccg
     8881 tggggaattc catcagccgg tcggcgtagt acagccagga aacgctgccg gtggcaagga
     8941 atgaagcgaa aatggtgttg atcagcagcg ataactgggc aatggacacg ccgaatacgg
     9001 ccggccccat ctggcgcagg atgcgcgaca cgccttcgtc gtggcgggaa aaccgcagcc
     9061 gcggcagcag tccgagccgg tgcaggaacg gcagctgcag cgccagttgc agcatgccgc
     9121 caagcaccac tccccaggcg agcgccatgg ccgggggatg gcaccaggga gccagccaca
     9181 gtgctgcgcc gatcaacgcc aggttgaaca gtaccggcgt aaaggcaggc acgccgaaac
     9241 ggctccaggt attgaggatg ccaccggcca gcgagaccag cgagatcatc aggatgtatg
     9301 ggaacgtgat gcgcagcatg tgcactgtca tgtcgaagcg ttgggggtcg tgaacaaagc
     9361 ccggcgcgct cagccagacc accagtggcg cggcaagcat gcccagcagt gtcaccgcag
     9421 tcagcgcaag cagcagcagg gtggtggtgt ggtcgatcag cagccgcgtg gcgtcgtgac
     9481 cgcgccgggc cttgtactcg gcgaggatgg gtacgaaggc ctgcgagaag gcgccttcgg
     9541 caaacaggcg ccgcagcagg ttgggaatct tcagcgcgac gaaaaaggca tcggtgtaca
     9601 ggctggcgcc gaagctgcgg gcgacgatgg tgtcacgcac gaagccgaag atgcgtgaca
     9661 gcagcgtcag gctgctgatc cgggccagtg cgtgcagcag gttcattggg gtgcgctcat
     9721 tggccggccg tgatcggctc aaggcgtgca taggccagtg cgagccattt ttcgccatgt
     9781 tcccggccaa aacgcacctg cacccgggca tcgtcggcgc caccttcggc gcgcaggatg
     9841 acgccgatgc cgaatttcgg atggcgcacc gacatgccgg gtggccatgc tggcgtggcg
     9901 ctgcgcgcgg ttgccgcgtc tgtcgggcgt gccaccgcgt gagccgtggc cacaggttcc
     9961 gcagcagtca cggccgggcg catcagtcca ggcgggattt cgtcgagaaa acgtgacggg
    10021 atgccatatc gggtctggcc atgcagcatg cgtacacgcg ccaggctcag gtagagcagt
    10081 ttgcgtgcac gggtaatggc aacgtacatc aaccgccgtt cttcctcgat gccgtgccgt
    10141 tcgttgagcg actgctcgcc ggggaacagt ccctcttcga gtccggtgag gaataccgta
    10201 ccgaattcca gccctttggc ggcatgcacg gtcatcagtt gcagggcgtc ctggcccgca
    10261 tcggcggcgt ggttgccggc ttcgagcgaa gccagcgcga ggaagtccga cagcacgtcg
    10321 tccgaaggca cataatgctc gtcctgaagg aacacacggg ccgactgaac cagttcatcg
    10381 aggttgtcga gccggtcctg gccttctttc tcactgcggt agtgttcgat gagtccgctg
    10441 gcatgcagtg tatgttcgat gatttccggt aatgccagtc cgcgcgtgtg ctcgcgcaag
    10501 gtgtcgatca gggtgacgaa accgcctaga ccgcggccac ccggctttgc atcagtgtat
    10561 gtacatgcct gccacaggct gcatccatgc gcgcgtgcgg tggcatcgag ttgttccagg
    10621 gtgcgggcgc cgatgccgcg ggtgggaaag ttgaccacac gcaggaaggc gctgtcatcg
    10681 tcggcatggg cgagcaggcg caggtaggcc aatgcatggc gaatttcctg gcgttcgaag
    10741 aatcgcaggc cgccatgcac ccggtagggg atgccggcgg aaaacaatgc atgttcgaga
    10801 acgcgcgatt gtgcgttgga acggtagagc acggcaatat ccgacagcgc ttggccttcg
    10861 ccttgcagcg ccagcgcgcg ttcgcagacg aatcgtgctt cctgctggtc atcaagggcc
    10921 tcgagcacgc ggatcggttc gccttgcccg gcggcagtcc acaaggtctt gcccagtcgc
    10981 cccgggttgt tgccgatcag cgcgttggcg gcgtcgagga tgtttccctg gctgcggtag
    11041 ttctgttcga gccggatcag gtgttcgcgc gcgaattcgc gctgaaaatc cagcatgttg
    11101 ccgctgtggg cgccacggaa ggcgtaaatc gactggtcgt cgtcgccgac ggccatcagc
    11161 gcgctgtgtt cgccggcaaa ctgcttgagc cagcggtatt gcaaccggtt ggtgtcctga
    11221 aattcatcaa tcaggacatg gtggaaccgt gtgcggtagt gttcacggat ttccgggtgg
    11281 cgttcgagca gttcgttgct gcgcagcagc agttcggcga aatcgacgac gccctcgcgc
    11341 tggcattgtt cgtcccatgc cgcatggagt tctgccatgc gccggttcca tggatcaccg
    11401 gtttccgccc ggtcggcgcg caggccgttt tccttgttct ggttgatata ctgctgcacc
    11461 ttgcgcgggt cgaagcggtc ggtgtcgacc tgcattgcct tgagcatgcg cttgatgccg
    11521 gcaagctggt cggcctggtc gagaatctgg aacaggcgcg gcagtccggc ggcggcatgg
    11581 tgagtgcgca gcaagcggtt ggacaggccg tggaaggtgc cgatccacag gtctcgtggc
    11641 gacatcggca gcatggcggt caatcgggtc tgcatttccc gcgcggcctt gttggtgaac
    11701 gtgaccgcga ggatgttgtg cgcgctggcc tgtccggtct gtaacagcca ggcgatgcgc
    11761 gtggtcagca cgcgggtctt gccgctgccg gcaccggcaa gaatcagcgc tgataccggg
    11821 ggcagcgtga cggcagcctg ttgttgcggg ttcaatccat cgagcaggtc gggcatggta
    11881 gacttcgggc ttggccgttg gggcaacgca tggcaaaggg gacattataa tggctaaaca
    11941 gtttggttgc ttcatcaagg aagcacgaac gcatatcgac gaatgggatg ccgagaccgc
    12001 gcacgagcgc gtgcagactg gcggggtgct ggtggtggac gtgcgcgagg ccgacgaatg
    12061 cgccacgggt cgtatcgccg gcgcgattca tgtgccgcgg ggcatcattg aggcggccgc
    12121 cgatccgggt acgcattatc gccatccgct gttgtgcgag gcacgccgcc agccggtgct
    12181 gctctactgc cagtcgggcg gacgctcagc ccttgcggcc tgggtgctgc agcagatggg
    12241 gttcgaggat gtgcattcgc tggcgggcgg actcgagtgc tggaaagccg aaggttacgg
    12301 gctggaatga gcgtgctggc ggtgacgctg ccgcgggcga caaccctttc ggtggtacaa
    12361 tagccggttt gatcgataac gcgtgaggat gccatggccg gccacagcaa atgggctaac
    12421 atcaaacaca aaaaagccgc tgcggatgca aagcgcggca agattttcac tcgcctgatc
    12481 aaggaaataa ccgttgcggc caagctgggt ggcggtgatc cggcgagcaa tgcgcgtctg
    12541 cgcctggcga tggacaaggc gtatgacaac aacatgccca aggacaccat cgagcgtgcg
    12601 atcaagcgcg gcagtggcga actggaggcg gtcaattacg aagaaatccg ctacgaaggc
    12661 tacggcccgg gcggagcagc ggtgatggtg gattgcctga ccgacaaccg cgtgcgcacc
    12721 gtggctgacg tgcgccacgc gtttaccaag ttcggtggca acatgggtac cgacggttgc
    12781 gtcgcattcc agttcaccca ctgcgggcag atgctgttcg cacccggcac gaacgaagat
    12841 gcgctgatgg aagctgcgct tgaggcgggc gccgatgacg tgcttgccaa cgatgatggc
    12901 agtatcgagg tcatcacgcc gccgtactcg ctgcatgcga ttcgtcaggc gctggaacag
    12961 gccggattca aatccgaatt tgccgaggtg agcatgaagc cgcagaatga agtcgagctc
    13021 agcggcgatg acgcagtgcg catgcagaaa ctgctcgatg cgctcgaggc gctggacgat
    13081 gtgcaggaag tgtatacttc ggcggtgatc gatttgccgg aatgacggcg ggaggcgcga
    13141 tgtttaccgg aatcatccag gcaacaggac gggtggcggt cgtggaaccg cgcggcggtg
    13201 atgcgcgcct cgaactggcg gccggtgggc ttgatatgac cgatgtggct gcaggtgatt
    13261 cgatcgcctg caatggcgtg tgcctgacgg tggtcggatt gcggccggag ggtttcctgg
    13321 tcgatgtctc cgccgagaca ttgcgctgca cctgcgggtt tcgtctgggg gatatggtca
    13381 atcttgaaaa agccatgcgg ctggaagacc gcctgggcgg ccatctggtc tccgggcatg
    13441 tcgacggtac cgcgcaggtg gtggctttcg acccggcagg cgagtgcatg gaactggtga
    13501 tccgtgcgcc gcaggagttg gcgcgttaca ttgtgacaaa agggtcggtg gcgatcgatg
    13561 gcgtgagcct gactgtcaac cgggtcgaca gcgctgattt cagcatcaac ctgattccgc
    13621 ataccctgca gaacaccggc ctgcaggtgt tgcggcccgg cagttgcgtc aatctcgaag
    13681 tcgacatgct ggcacgctac gccgagcgca ttctgaatta ttctccgcgg gcctgagacc
    13741 cgcaggtggt cagcccgtct gtccgcttca ttcagtcagg aacagaatca tgagtttgag
    13801 tccgatcgaa gacatcatcg ccgacatcaa ggccggaaaa atggtcatcc tggtggacga
    13861 ggaagatcgc gagaatgaag gcgatctgct tatggctgcc gaatttgcca cgccggaagc
    13921 cgtcaatttc atggtgaccc atgcgcgtgg tctggtgtgt ctgacactga cggacgagcg
    13981 ctgccgccag ctgggtctgc ggcagatggt cagcgataac cagacgccgc acaatacggc
    14041 gttcacggta tccatcgaag cggccaccgg cgttaccacc ggcatttcag cagccgatcg
    14101 cgcacgcacc atccaggcgg cggtagcgcg gaacgcacgg ccgtcggaca tcatccagcc
    14161 ggggcacatc ttcccgctgc gcgcccagcc gggtggcgta ttgatccgtg ccgggcatac
    14221 cgaggccggc tgtgaccttg ccgcgctggc cggactggaa ccggcggctg tcatttgcga
    14281 gatcatgaag gaagatggca gcatggcgcg tttgcccgac ctggtcggct atgctcgtga
    14341 gcatgggctc aagatcggta ccattgctga cctgatccat taccgcagcc gcaacgaatg
    14401 tctggtcgaa cgcgctggct cgcgcctgat tcgcacgcca tttggcgagt tcggtctggt
    14461 ggtctaccgc gaagtggtca ccggggcagc gcatctggcg ctggtcaagg gcgagatcag
    14521 tcccgaccag ccgacgctgg tgcgggtgca tgaaccggtg tcggtgatgg atgtgctgga
    14581 actggacagt tccatgcaca gctggagcat cggggaggcg ctgcgcagga tcgatgcggc
    14641 cggcagcggg gtgttggtgc tgctctaccg gcaggaaacc gccgatgatc tgctggagca
    14701 tgccttgcct gacggccagc cgcgcgctgc gcagaaatgg gacgcgcgca cctatggcac
    14761 cggcgcacag atcctgcgtg atctgggtgt gggaaagatg cgtctgctgg cagcgcggcg
    14821 caggatgcct tcgctggccg gtttcggcct ggaagtgctt gaatacgatg agacccacgg
    14881 ctgacatgcc gcaacgcaag gaaaatgaca tggcccgtta tgacggaatc cacgaaatcg
    14941 aacccgtgct ggatggcagc gggctgcgca tcgcgctggt gatgagccgt ttcaaccacg
    15001 atatcggcga tgcgttgctg gccgcctgcg tgcacgcact ggacacgcat ggcgtccgca
    15061 gcgaagatac gctgctgctg accgtgcccg gggcgctgga aattccgctc gcggcgcgca
    15121 agctggcgct cagtggccgt tttgacgccg tggtcgcgct gggcgcagtg gtgcgcggtg
    15181 atacgtatca tttcgaggtg gtgtccaatg aaatggccag cggcattacg cgggtgcagc
    15241 tcgataccgg cgttcccgtg gccaatgccg tgttgacgac cgataccgac gagcaggcgc
    15301 atgcgcgtac tgcggtcaag ggagcggaag ccgctgcctg cgccatcgag atggccaacc
    15361 tgatgaggat ggtatgagca acccccgcca ccatgcgcgc gagctggccg tgcaaggcat
    15421 ttatcagtgg ctgctcaacc gtcagccggt gacgcagctg attgcgcagt tgccggctgt
    15481 tgcggatgat gatgccgaag cattcgacga gccgctgtac catgccctgc tgcatggcat
    15541 cgtggcgcat gccgacgagc ttgatgccca gctcgcacgt ttcgtcgacc ggccagtgac
    15601 cgccctgagt ccggtggaac acgccgtcct gctgctgggc gcggaagaac tggcacacca
    15661 cccgcaagtg ccgtaccgtg tggcgatcaa cgaggccgtc gaacttgcca agacgttcgg
    15721 cggtacggac ggccacaagt acgtcaacgg cgtgatggac cggctggcgg tcgcgctgcg
    15781 cggtgcggaa gcaggtagcc gcgcccggcg cggttgacac ggcggcagga cggatgatgg
    15841 tggccggaac ggaattttcg ctgatccggc attatttttt gcggccggcg cacagcgccg
    15901 tgctcggcat gggtgacgat gctgcgctgt tgcagccgtc tgccggccag ttgctcgccg
    15961 tatccaccga tacgctggtc agcggtgtac atttttttgc tgatgccgat gcgcgtttgc
    16021 tgggctggaa agcgctggcg gtcaatctgt ccgatctggc cgccatggga gcggtaccgc
    16081 gttgggccac gcttgcgctg accctgccgg ccatcgacga aggctggtta tctgcctttg
    16141 ccgctggctg gcacgagtgt gcgcaacagg ccggtatcga tctgattggc ggcgatacca
    16201 cgcagggacc cctgacactg acagtcaccg tgctgggtga agtgccggct ggacaggcct
    16261 tgcggcgtga tgcggcgtgt gccggtgacg acatctgggt atcgggcacg ctgggtgatg
    16321 ccgcgctggc gctggcggtg ttgcaggacc ggctgcaggc cgatctggca gaccgggccg
    16381 tgctgaccgg ccggttgcat cagccgcagg cgcggctgac gcttggcgtg gcgttgcgcg
    16441 gtctggcgca tgctgcgatc gacatatccg acggattgct cgccgatctc ggccatatcc
    16501 tcgagcgttc cggagtgggg gcaaccatcg aatttgcgca tgtaccggtc agcccggctg
    16561 cggcgcgttt tgcgttacac ccgctgtatg ccggctgcgt tctggccggg ggggacgatt
    16621 atgaactgtg ttttaccgcg ccagccgctg cggccacgac gattgaagcc ctgagcggtc
    16681 ggctgggagt ggcgttgaag cgcatcggtc gcatcgaagt gcagccgggg ctgcgtgtgc
    16741 tcgacgcggc aggcaggacc atgcagaccg gacggatggg atatgagcat tttgcagcct
    16801 gactggcggt ttctgcggca acatccggcg cacatgattg cgctcgggct gggcagcggg
    16861 ctgtcgccgg tggcgcctgg tacggcgggc accttgatgg cggtcccgtt gtacgcggca
    16921 ctggctgcac tgctgccgcc tgccggactg tggctggtca ttgcgctgtc cctgccgttc
    16981 ggggtgtggg cctgtggcgt ggccggtcgc catctgggcg agatcgacca tgggtcgatc
    17041 gtgtgggacg agattgtcgc tttctggctg gttctggcct gcatcccctg gcgcatcgct
    17101 ccggtggtgg cggcatttgt gctgtttcgc ctgttcgatg tgtggaaacc gtttcccatc
    17161 ggctggctgg accggcatat tcataccccg ctgggtgtga tgctcgatga cttgctggcc
    17221 gcaggatatg cgctggcgat actggaaggg gggatgcgat ggctgcagtg atccccgggc
    17281 ggcgcccgga cgatgccgaa ctgtttgccc tggcgcagca gttgggcgag gtgctgaagt
    17341 cgaaatcgtt gatattgacg accgcagaat cgtgtaccgg tggctggatc ggcaaggtca
    17401 tcacggcggt tccaggcagt tcggcgtggt acgagggcgg tttcatcacc tataccaaca
    17461 ccgccaagat atcccagctg gatgtgcgcc ccgaaacgct tgcgtcatac ggcgcggttt
    17521 cggagccggt ggtgcgtgaa atggcgcagg gtgcgctgca atgtgcccag gcgcaaatcg
    17581 cggtcgcggt ttccggcatt gccgggccgg gtggtggcac gccggagaag ccggtcggaa
    17641 ccgtttgcct gggttggatg caggtggcag gaacgcagcg tgaacgcacg tttcgttttg
    17701 acggcgaccg cgaagcggtg cgtgctcagg cggtggccgc agcattgcgc ggtctgcttg
    17761 agttgctggc agactgacag tagtatctca atacttgata atcggagtga cacatggatg
    17821 aaaaccgcag caaggcgctg gcggcagctc tggcgcagat cgaaaaggct tttggcaagg
    17881 gttcgatcat gcgactgggc gatggcgaag tggaaaacga catccaggtc gtgtccaccg
    17941 gatcgctggg actggacatt gcgctgggtg tcggtggttt gccgcgtggg cgcatcgtgg
    18001 agatctacgg gccggaatcg tctggcaaga ccacgctgac gctccaggtc attgccgaaa
    18061 tgcagaagat gggcggcaca gcggcattca tcgatgccga gcacgcgctc gatccgcaat
    18121 atgcgtcgaa gctgggcgtc gataccgcca atctgctgat ttcccagccg gacacaggcg
    18181 agcaagcact ggaaatcgcc gacatgctgg tgcgctcggg ctcagtggat gtggtggtga
    18241 tcgactcggt cgccgcactc acgcccaagg cagaaatcga gggcgagatg ggtgattcgc
    18301 acatgggcct gcaggcccgg ctgatgagcc aggcgttgcg caaactcacc ggtcacatca
    18361 agcgcaccaa cacactggtc attttcatca accagatccg catgaagatc ggcgtgatgt
    18421 tcggtaaccc ggagaccacg accggcggca atgcgctcaa gttctatgcg tcagtccgta
    18481 tcgatatccg tcgcaccggc gcgatcaaga agggtgaaga ggtcatcggt tcggaaaccc
    18541 gggtcaaggt ggtaaaaaac aaggtcgcgc cgccgttcag acaggccgaa ttcgacattc
    18601 tgtatggtga gggcatttcg cgcgaaggcg agatcatcga gttgggcgtg cagggcaaac
    18661 tgatcgacaa gtctggcgcc tggtatgcct ataacgggga aaagatcggc cagggcaagg
    18721 acaatgcgcg cgaatacctg cgtgaacatc cggaaatcgc gcgcgagatc gaaaatcgtg
    18781 tgcgtgccgc tatcggtgtg catgcggcac cggcgatgcg ggtggacgag gacggtgtca
    18841 tcctcgaatg acctcgccgc cgcacaagcc ggcggcgatg acgctgcgcc agcgtgcgct
    18901 ggccctgctg gcgcgccggg aatacagccg gaccgaactg gcgcgcaggc tggcgcagca
    18961 ggacggggtg tccgccgagg cgctggaggc attgctcgat gcgctggaag tgcggggctg
    19021 gttgtcggac gcacgttacg cggaacagcg cgtgcatgcg cgtcaggcgc gctatggcag
    19081 tcgcaagctg gcgctggagt tgcgcgagcg tggcgtggac ggggcggtaa tcgatgccgc
    19141 gttgctcggt ttgcgcgaca ccgaactggc gcgcgcgcgt gcggtctggc aacgtaaatt
    19201 tcccctgccg gcccaggacg ccagagaacg tgcccgtcag gtgcgatttc tgcaagcgcg
    19261 cggtttcggt tttgatgtca tcaggcgtgt gatcgacggg ctggacgcaa ccgacgacgt
    19321 gggctgatgc cgatgcgttg cgctcaccag ccgagccagc gtgctgtctg gtagaaaatg
    19381 aaactcacca gccaggcgag actcagcggc cacagcaccg cgagcacggt aaatcccgta
    19441 cttttcgatt cctgacgcaa gacggcgatg gtcgacaggc acggggtata gatgagcgtg
    19501 aacagcatga agctcatggc ctgaacccag tcgatctggt gtgcaagcag gctggccagg
    19561 ttgttttctc cagcaccata gatcacggcc agagcaccga tgaccacctc cttggcaacg
    19621 aagccgaaca gcagtgtgat cgacagcagg cggtcaatcc cgagcggact gaataccgga
    19681 cgcatccagt cggccagtcg tccggcgagc gtgccggcgc tggccggagg caggtcgaag
    19741 ggaatatgcg tcagcgccca gaccaggatc acgccggcga caatgaagcc ggaagcgcca
    19801 cgcaggaaat gccccgactg ttgccatgcc tgggccagca tttgccggcc ggtagggaag
    19861 cgataaggcg gcagttcgag cagcagcggt tcgtggttac ggaacttgcc gcgccacacc
    19921 agtgcagtca ggaaggcgac gaggaaactg gtgatataca ggccaaacat gaccgaggct
    19981 gcggccaccg gcttgaagat ggcgctgctg aagaagagga acacctgcag ccgggccgaa
    20041 cacagcgaaa acgggatcac cagcatggtc agcaaccgca gcgcgcgtga acgcatgacc
    20101 cgtgtaccca ttaacgccgg cacgttgcag ccgaaaccca tcagttgcat gacgaacgaa
    20161 cgaccatcca gaccgagccg ttccatcagc gcatccatca gccaggctgc gcgtgcaagg
    20221 taaccgctgt cttccacgat ggacatgaac acgaaaaaca ccacgatgat cggcaggaaa
    20281 ctcaatacgg tgccgatgcc gttgaacagt ccgtcggtga tgaagctgcg cagggccagc
    20341 ggcgcatcgt gcagccaggg gttgagcgcg tggttctgga accggtcgag cagccagctg
    20401 atgccgtcct gcagcggggt gccgatgccg tagacgagct ggaacatggc gaacatgctg
    20461 aggaaaaaca gcggcaatcc cagccatgga tgcaggaaaa tcttgtccag ccggcgcgtg
    20521 aggttggggg ggagtgtaac cggggtggtc acggtttcac caatgatgcg atcgagttcg
    20581 gcctcgatgc tgtcatcctg ctgtagcgcc tcggcgctgg ccagtcgggc cggcgtggcg
    20641 gaattgagca gggattccag cgcggtacgg gcctgaccga agccgttgcc atatttggcg
    20701 ctgaccagcg tgacctccat gccaagatcc tgttgcagcc gttcgcggct gatgtgaatg
    20761 ccgagctggc gggcctcatc gctcatgttg agtatcagcg tcagcggcag tccgagcgtg
    20821 cgcagctgca gcagcagcgc gagctggcgg tcgagttggg tggcgtcgag aatgaccacc
    20881 aggccgttga gtggctgcga ctcgaggaaa tggcgcacca cgtgttcgtc atcggaaaag
    20941 ccatgcaggt tgtagatgcc gggcaggtcg atgacttcca ccatgccacc ggccagcagg
    21001 atcttggccg acagcaggtc gacggtgatg ccaggccagt tgccgacccg cgctcccgct
    21061 ccggtcagac gattgaagaa agtggacttg cccgtgttgg gcatgcccag gagcgccagg
    21121 cgtttcatcg tttcctcagt gatcggtttc cacgtggatg cgcgcggcgt cgctgcgccg
    21181 gatcatgatg tcggtgtggc cgacacggat ttgcaggggg ccattccatg gcgcggcgcg
    21241 aatgacttcg acgctgcggc ccggcctgag tccaagcgca gacatgcgcc gggtcaattc
    21301 cgggctcgcc tggatggcgg tgatgacacc gctctgttcg ggaaacaggt ggtcaagcga
    21361 gttttgcatt accgatcctg aatgagaagc atttttattt atagcacgtt ccagccttgc
    21421 gcgcaacgct tcaccgggcg acgtggtcac tggccgccgc attgcgggcc acaggctgtg
    21481 aagcggttgc cctgcgtacg tcgcaggacc gccgttgcga acaatgaatg cactccggtc
    21541 atgatgtgta atcggcgcag cgaaactgtt gctcatgcag cacgacatcg tcgcaaccgc
    21601 atgtagcctg tcagcatacc cgatttgtag gctattgtgg gcagtcccgt gtcggtgtcg
    21661 tgcaaaatgg aataaatcgt caaaaaaccc gtcatggcgg tgcgctacgg tggtaacacg
    21721 gcgatgtggg tatgttatgc tgaacaaaac tgcgcatgca gcggaataca gtcagcgatc
    21781 ctggcgacag aagatgacat ccaaatccta tatttcgcgc tggcacgatc cccataacac
    21841 actgggatca caatccgtca atctggtggc gggggatatc ggcggcacct ggagccgact
    21901 ggcctggttc agccaggcgt ggcaacaacc acgccgcatc gcttttgagc acgtctatcg
    21961 cagcgatgca ttcaccgatg ccatccattt gctgcgaacc ttctttgtcg atgctgcaca
    22021 atccggcctg ccggaacgaa tctgtctggc gttgcctggc ccggtcgatg aacagcgcgc
    22081 ggcgctgacc aacctggact ggatcgtgga tgcgcagcag attgccactg agctgggtct
    22141 ggccgaagtc tgtttcgtca atgatttcca ggccgctgcc gccggtgtcg aaagcctggg
    22201 gcgggccgac ctgcgcgtgc tcattcccgg ggtgccccgc ctgggtgcca cacgggtcat
    22261 caccggagcc ggcaccgggt tgggggtggc gtggatgcaa tcggacgagc ggggcaactg
    22321 gcaggtgttt gccagcgagg gcgggcacgc tgattttgcc ccgaccgatg cgcgccaatg
    22381 cgagttgctg cattggctgg ctcggcgtca tggcgggcat gtgtcctggg aacgcgtcct
    22441 gtcgggcggc ggattgtctg cgctgcatgc gtttgtctgc ggccgcgacc cgatcacgga
    22501 tgaacaggat atcgatcctg cgcgcattca cgagctcgcc ctgcagggtg ggcgggaggc
    22561 ggtgcaggcc atccagctgt tcgccgacgt ctatgcggcc tgggtgggaa atcttgcggt
    22621 gctctaccaa ccccggggcg ggttgtacct tgccggaggc atgtccattc atttgcagga
    22681 ctggctgatc gcacagcgtt tcgtggaact cgcgatcgcc aaaggacgca tgtcggccct
    22741 ggtcacgcaa acgccggttt acctggtgac ccatgggcgc gtcgggttac tgggcgccat
    22801 gcacctgtgt acgagcaggg ctgcggcgct gggttgccgc ccggcacgat tctcgcgtca
    22861 ataaacacca ataagcatga ggaggcgttc atgaccatca gcaaggaaca acaggaaaaa
    22921 ctgtatcgct actacacaga accaaaaatg gtcacggacc tcacgcggcg caccatggtg
    22981 ctggtgctgg ccggtggcga gggctctcgg ctgaagaacc tgactgcctg gcgtgccaag
    23041 ccggccgtgc cgttcggcgg caaataccgc atcatcgatt tcgcgttgtc caactgcgtc
    23101 aattccgggt tgcgccgcat cggcgtgctg acgcagtaca agtcgcactc gctgatccgc
    23161 cacctgcaac gcgcctggag tttcatgcgc gccgagatcg gcgagtttgt cgagattttg
    23221 ccggcgcagc aacgcacgca caagaaggaa tggtatcagg gcacggccga cgcgctgttc
    23281 cagaatctgg atatcctgcg gcggcacaat ccacagtatg tcatcgtgct cggtggggat
    23341 cacatctaca ccatggatta ttccgccatg ctgctgcacc atgtcgagac gcgcgccgat
    23401 tttaccgtag gttgcatcga ggtaccgcgc gaggaggcca cggagtttgg ggtgatgtcg
    23461 gtggatgacg agatgcgcat cacccggttt accgagaagc cggcccaccc cgaggccatg
    23521 cccgacaagc cgggtacggc gctggcctcg atgggcattt atgtgttttc gacggaattt
    23581 ctcttcgata tcctgatttc ggatgcggag catgaaaatt cgtcgcacga tttcggcaag
    23641 gacatcattc ctgccaatat ccataaggcg cgcgcgattg cctacccgtt ccgcaagccg
    23701 gatggcgagc cgggctactg gcgggatgtg ggcacggtgt actcctactg gaaggccaat
    23761 atggaattgt gcgccatcga tccggaactt aacctgtatg atcgggactg gccgatctgg
    23821 acctatcagc cgcaatcggc gccggccaag ttcatttttg acgacgaggg ccggcgtggc
    23881 gaagccatcg actcgctggt atcggccggc tgtatcatct cgggtgcgcg gctcaagcgc
    23941 tcgatggtgt tctttggctg ccacatcgag aattacagcc tggtcaagga cagcgtgatc
    24001 ttgcccaagg tcagcatcgg acgcaattgc cgcatatctc gggcagtgat cgacaaggcg
    24061 acggtgattc cggacaatac ggtcatcggc gaaaatctgg tggaagacgc caggcgcttt
    24121 catgtaacca aagaagggat cgtactggtg acgccggaaa tgatggggca gaacctgtac
    24181 cgatgacggg aaccgaatga acagtcgact gaatgtggtg ttgtgctggc acatgcacca
    24241 gccctattac cgtgacggtc tggagggcga gtatcgtctg ccgtgggtat acctgcacgg
    24301 aatcaaggac tataccgaca tggttgcgca tctggagcgg catccgctca tgcgcgccgt
    24361 ggtgaatttc acaccggtat tgcttgagca gatcgacgat tattcgcaac agatcgaccg
    24421 ttttctcagc gacggcaggc cgactcagga ccgcatgctc aacctgctgg ccggcgtgga
    24481 gccgattccg tccgatgcgc ccgggcgcga gcagttgttg cgtgattgcc agcgctgtca
    24541 tgccccgcgc atgattcatc cctatccacc attcagccgg ctgttgcggc tggcaggggt
    24601 gtccgaagac ccggccatca gcccggacag tggccagcac ctgctcaggt atctcggtga
    24661 ccagtatttt cttgacgtac tcacgtggta tcaccttgcc tggctgggcc acagcctgcg
    24721 cgatcatgcc gatgtccgcg aactgatgga gcgcgcagag gactttaccc tgcagtatcg
    24781 ccgcaagctg gtggttctga tacgcgatgc gctgtcgggg ttgatggccc gctaccgtgc
    24841 gcttgccgac cgtggccaga tcgaactgtc gatgacaccc tacggccacc cgatcattcc
    24901 gctcatggat agtttcgaca acatgaactg ttccatgccc gaagcaccgc atcctgaatc
    24961 ggagacgtat cccggtggcg aggcgcgcag cgaatggcat gtggcgcacg gcatcgaggt
    25021 gttcgagcag tatctgggac gcaagccggc cggagtgtgg ctggccgagg gcgggctcag
    25081 cgaggatgcg cttcgtctgc tcgaccggca cggcatccgc tggacggcgt ccggcgaggg
    25141 cgtgtggcgc aacagctgcc ggctgtcagg catcgagccc ggcatcatcc agagcaagaa
    25201 gccgctgttt tcacccaatc tgaacccgga tgcctcggta cggatgtttt tccgcgatga
    25261 cgggctgtcc gacctgatcg gctttcgcta taacaactgg catgccgaag acgctgtcgc
    25321 cgatttcatc cagcacctca acaacattgc cgcgtttctc ggcgccgagg cagagcagca
    25381 cgtggtgtcg gtgatcctcg atggcgagaa cgcctgggaa tattatcccg ataacggcca
    25441 ccatttcctt gacgggctgt attcggcact ggagtcttcg cagcgactgc gcgtcagcac
    25501 gtttgccgac tgcgccgagc gcctgccgcc cacggcgttg ccgcagctgt gtgccggcag
    25561 ctgggtgtac ggttcatttt cgacctggat cggatcgccg gacaaaaacc gcggatggga
    25621 ttacctcgtc agcgccaagc gtgcctgtga tgaggtactg gcccgtgctg gcgatgatga
    25681 ggcgcgcgcg ctgcagatcc gtcgccagct ggcgatttgt gaaggttcgg actggttctg
    25741 gtggtttggc gactacaatc cggcagatag tgtcagtgat tttgaacggc tgtatcgcga
    25801 gcagttgcgc gcattgtatg tcatgcttgg cgaggcaccg ccggcgcagc tcgatgttcc
    25861 gttgtcgcgc ggcggcgggc aagcggagaa cgccggcacc atgagaagga atgcgtaatg
    25921 aacccatggt tgtccgggcg ccgtgccggc gtgctgctgc atacgagttc actgccgtcg
    25981 ggacggctgg atgccgacgc ctggcgctgg atcgactggc tggccgaagc cggcatgtcg
    26041 gtgtggcaga tgttgccgct cggtgtgccg ctgtctggag tttctcccta tcagtgcgca
    26101 tcggcgtttg cgctcaatcc cgccctgttc ccgctggaat tgcctgcggt acgcatcagc
    26161 tcgcgacaat gtgtcgagtg gcgcgaacgc gaacgcgcgt ggatcgagga ttatgccctg
    26221 ttcatgacca ttcgggagaa ataccacaat gcgccctggg tgacctggcc cgaaccgctg
    26281 cgcctgcgga aagcggatgc cctgcgcgag ttcgccgcaa ggcatgaacg tgcgattgcc
    26341 cgccatgtgc gccagcagta ccagttgttc gtgcactggc aggcgctgcg atctcatgcg
    26401 gctgaacgtg gtgtggcgct gtttggcgac atgccacttt ttgtcgcgca tgacagcgct
    26461 gatgtctggg cgcatccgga gtgtttcctg ctcgatgctc gcggtcagcc cacgttcgtt
    26521 gccggcgtgc cgcccgacta tttctctccg accgggcaac gctggggcaa tcctcagtac
    26581 gcatggaacc atctggaatc cgatcatttt tcctggtggt gcgaacgatt gcgttatcat
    26641 ttcgaatgtt tcgacctcgt gcgcatcgat catttccgtg gcctggtggc ctcgtggatg
    26701 atcccggcat cccacgacac ggccactgaa ggctactggc aggcggtgcc gggcagggaa
    26761 ctgctgcagc agatacacga tttgatgggc gatctgccgc tggtggccga agatcttgga
    26821 gtgatcaccc ccgatgtcaa cgcgttgcgc gaacgattcg gcttgccggg aatgagtgtg
    26881 ctgcagtttg cgttcgacag ttttcccgac aatccgcaca aggtggaaaa tgttgatctg
    26941 gaccgggtgt attacaccgg cacgcacgac aatgacacca ccgcaggttg gttcgacaag
    27001 cttgaagcgc cggtgcgcgc cgacgtgatg gctgcgcttg gtgttgaaca gcccggcgag
    27061 gtcgtggcgg cgatgcgcat gcgcgtattg tccagtcgcg cagcactggc catgctgccg
    27121 atgcaggacg tgcttgggct ggatggtcat gcgcgcatga acacgcccgg caccgtggat
    27181 ggaaactggc tgtggcgttt tggctgggag cagctcccgg ccggactcgc cgcttcgttg
    27241 catgctcaat tggaacagac aggacgtttg tatcatgcct gacagcatta cccgcttggc
    27301 gatgggacgc catcacgatc cgttttccct gctcgggtgg catgcccaga ccgggggcgg
    27361 ctgggtattt cgtgcctgga tgccgcaggc ggaacgcctg cgctgggctg acgggcagga
    27421 attgacccgt cgcgatggca ccgatctgtt cgagctggtg ctggcggaaa aaaccgttca
    27481 gcatccggta ttgcagtggc tggacaagga cggcggtggc tggcgcgaca cggtgtcgcc
    27541 ctataccttt gcaccccagg tcggcgagct cgacctgtat ctgttcggtc aggggcgtca
    27601 tcatcatctg tggcgcgtgt tcggcagcca tgtgcgcagc attgacgagg tgtcaggcgt
    27661 attgttcgtc gtgtgggcgc cgggcgcgca gcgcgtaagc gtggtagggg atttcaaccg
    27721 ctggaatggt ctgagtcacc cgttgcgggt acgcggcgaa agcggggtgt gggagctgtt
    27781 cgtgccgggc ctgacctccg gtctggccta caagtacgag atcatcggtc cgcatgggca
    27841 gttggccatc aaggcagacc cggtagcacg gcagacgttc ctgcgcccgg agacggcctc
    27901 atgcattgcg gcggattcgc tgtacacctg gatggatgcc gactggatca ccgcgcgcga
    27961 gcgtttcgac tggcaacatc agccagtcag catctatgag ttgcatacgg gctcgtggtg
    28021 gcgacatgtg gatggccgct ttcttgactg gcatgagctg gccgaacgac tgattccgta
    28081 tgtgcagtcg cttggctata cccatatcga gctgttgccg gtggccgaac atccgctcga
    28141 ccagtcgtgg ggctatcagg tcagcggcta ttttgccccg acggcgcgtc atggcaagcc
    28201 tgatggcctg cgcatgttca tcgacgcctg tcatggcgcc ggcatcggcg tgctgctcga
    28261 ctgggttccg gcgcatttcc ctcgtgatga ctttgcgctg gcgcgtttca ccggcgagcc
    28321 ggtctatgag catgccgatc cgcgccgggg cgaacaccgg gactggggga cgctgatttt
    28381 cgattatggc cgtcatgaag tgcgcaactt ccttattgcc aacgcattgt actggctgga
    28441 agaattccac tttgacggtt tgcgtgt
//
LOCUS       DAIANL010000083         6206 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_917740, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000083 DAIANL010000000
VERSION     DAIANL010000083.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917740"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1381 a         1944 c         1813 g         1068 t
ORIGIN      
        1 gcgcgatttc caccaggccg ccgacgctga tcgccagcat ggtcaacacc gccatcaggc
       61 cgatgttggt ttcaatccat tcgtgtttga agttgaagtt catggtctat ctttcccgaa
      121 tcatgcgtga gccagcttgc ttgccagctt ggcttccagc gcagcgcttt cgcgtttgcc
      181 ctgacggatg gtcatgaaca tgttgaaggc catcgtcaac atgcccgtca ggaagaacat
      241 gccgccgatc gcgcgcatcg catagaaggg atgcatggcc gcaaccgact cgacgaacga
      301 gtactgcagg ttgccgaact ggtcgaatgc acgccacatc aggccctggg tgataccgga
      361 aatccacatc gcgacgatat agagaacgat gccgatggta gccagccaga aatgccattc
      421 gatcagtttg cggctgtaca tcttggtatc ccacagcttg ggcatcatgt gatacagcga
      481 accgaaggag atcatcgcca cccagcccag tgcgcccgag tgaacgtggc ccacggtcca
      541 gtcggtgtag tgcgacagcg cgttgacttc cttcagcgac atcatcggac cttcgaaggt
      601 cgacatgccg tagaacgaca gcgccacgat catgaagcgc atgatcgggt cggtgcgcag
      661 cttgtcccag gcacccgaca gggtcatgat gccgttgatc atgccgcccc acgaaggcat
      721 cagcagcagg atcgagaatg cagcggccag cgacgaggtc cagtcgggca gcgcggtcca
      781 gtgcaggtgg tgagcgccca cccacatgta catgaagcca agcgcccaga agtggacgat
      841 ggacagacga taggaataga tcggacggcc ggcctgcttg ggcacgaagt aatacatcat
      901 gccgaggaag gccgcggtca ggaagaagcc caccgcgtta tggccatacc accactgcgt
      961 catcgcatcc tgcacgcccg agaacaggct gtaggacttc atcgagaaca ggttgatggg
     1021 caccgccagg ttgttgaagg tgtgcagcag cgcggtcgcc agaatgaacg ccaggtagaa
     1081 ccagttggcg acatagatgt gcggctgctt gcggcgcatg atggtgccga cgaacaggat
     1141 caggtaggtc acccacacga tctcgatcag gatgtcgatc ggccactcca tttccgcata
     1201 ttcgcgcgac tgcgtgtagc ccagcacata gctgaaatct gccagcgcga tggccgccat
     1261 ccagccccaa aaggtgaagc tggccatgcc gtccgaaatc aggcgggtct ggcaggtacg
     1321 ctggaccacg tagtaggagg tggcaaacag cgccgagccc ccaaagccga aaatcaccga
     1381 ggtggtgtgg accggacgga atcgcccaaa cgaaaagtac gggctatcga agttcaggaa
     1441 cggccaggcc agttcggagg cgatatacac gccgacgaac atgccgacca ccgcccaaac
     1501 gagcgacatg acggcaaatt tcttgatgac ttcaaagttg tactgctccg caccggaata
     1561 tgtggttacc gccatggacc gctcctgttg tacccaaaat agcgcctgtt tctgcgcctt
     1621 aacagcatat ggttatatta aaaaactgcg ggattatatt ctgcccggcc ggcgtcccgc
     1681 aagcccaaac tcggttcagg aaccagccag cagtatcttc agatcgtgtg cgatcgcttc
     1741 ggggccgctg ccgtagggca tgagcagtct caaatgccca tttggatcat agatataggt
     1801 gccggcgctg tggtcgatca ggtaatcgtc tgctgctttt accgatgtct tttgatagac
     1861 cactttgtac tctttcgcca actgcttcag cgtgtctgga tcggtgtaca tgccgaggaa
     1921 gctcggattg aaggcgggga caaactgctt cagaatatcc ggggtgtcac gctgcggatc
     1981 cagcgtcacg aaaacgacct gcacgcgccc ggcctgtggc cccagtaatc tgagcgcagc
     2041 ggcgaagtcg gacagcgtgg tgggacacac atccggacaa tgcgtgtagc cgaaaaaaac
     2101 ggccacgacc ttgccccgga aatcggccag cgtgcgcacc cggccattgt gatcggtcag
     2161 tcggaaatcc cgcgcaaagt cagcgccggt gatgtccgtc gcctggaaca cgggcggctg
     2221 atgggcgggc tgacaaccgg cgagcgcgag tacggctgcg gtccagacag cagcaatcca
     2281 gccggttgtc atggcttgag ctgctcgaac aaggtcccgg gaacatccgc cagccggtat
     2341 tttccggatt caaccgcctt cgccagcttg tccagcgtgg tgtcctgcag aagatcgatg
     2401 attttcttct tcacgggaac ccagcggaaa tgcagcgggc acgccgtttc atcgctgcat
     2461 tttttcagcc ccagcaggca actctgggtg aatgccgggc cttcggtcaa cagcaggatc
     2521 tgcatcagcg tgaccttgtc gcccccctca cgcaaacaga agcccccgag acggccgcga
     2581 aaggaaaaca gcaggttgcc cttggccaga ctttgcagga ttttggccag atacggcgcg
     2641 ggcacaccca gtttggcggc gatatccttg ttcagcaccg gcgtggcgtc aggctgggta
     2701 gccatgtaga tcagtgcctg gacggcatac tgactggtgc gtgagaggat cattctgtgc
     2761 cccgacaaaa catactgggc acaatataaa acaatcctgt ccgaatatcc agttagaaga
     2821 tcaaatcccc ataataatca aacgggtaac gggaaataat gatcaaccag catgaccgca
     2881 aacagcacgc tcaaatacca gatggaatag cggaacgtct gctttgccag cgcatcgctg
     2941 tattcgcggt acagacgcca gccgtactgg ataaaccggc cgcccagcag aacggcgccg
     3001 accagataga tccatccgcc catgccggtg ccgaccggca acatggtcac ggcgaacagc
     3061 aacagggtgt agagcagaac atgcagtcgg gtgtaggcct cgccgtgcgt caccggcagc
     3121 atcggcaagc cggcccgggc gtactcctcg cggcggtaca gggcaagcgc ccagaagtgc
     3181 ggcggcgtcc aggcaaaaat gatgaggaac agcaacaggg catcgtggtg aatttcgccg
     3241 gtggccgccg cccagccgag caccggcggc atcgcgccgg aagcgccgcc gatgacgata
     3301 ttctgcggcg tggccggttt cagtatcgcc gtatagatca ccgcatagcc gacaaacgtg
     3361 gccagcgtca gccacatcgt gagcgggttg acgaacgcgt tcagcagata caagccggca
     3421 ccgcccacca cgccggaaaa caccagcgtc tgggtactgg tcagttcgcc gcggggcaac
     3481 gggcgcgcgc gcgtgcgcgc catcacggcg tcgattttct gttcgaccag gcaattgaac
     3541 gccgcggccg cgccggccac cagcgcgatg ccgagcgtcc ccgcccatag cgcgttccag
     3601 tccggcaagc cgggcacggc gagaaacatg ccgatcacgg ccgtgaacac gatcaggctg
     3661 accacacgca acttgcacag cgccagaaac tgctggcaac gacaggctgc gccctgaatc
     3721 atcaaggatt ccatactcat ctcctgcgta cccgataatt cagtatcacc agactggata
     3781 gcagcagggc cgcgccggcg ttatgcgcga ccgccactgc caacggcaga ctgaacacga
     3841 cattgctgat gcccaacgaa acctgcagaa ccaacaggcc gcctatggcc aggcccatgc
     3901 cggcgtaacc cgggctgcgc accaggcgca gcaccagcca gctcagatac agagccacca
     3961 ccagggccat gacgcgatgc gtccagtgaa tagccgtcag tgccgtcaac ggcaacaatt
     4021 cacccgtagc cgtctcgccc aggttccggt gcagggtgaa tccctgctcg aaatccatgg
     4081 gcggcagcca cgcgccgtga cacatcggaa aatcgggaca ggccagggcc gcatagttgg
     4141 tgctcaccca gccgcccagc ccgatctgca ccgccaccag caccagtccc agcaaggcag
     4201 cgccgcgcag atgatcgctg ttcgcataat aggtgtgggc gtattgtcca cgttcgcgca
     4261 accgcagcca taacagcagg gacaatgtcg tcattccccc cagcaaatgc gagctgacaa
     4321 tcagcggctt cagcagcttg gtcaccgtcc acattcccag catcgcctga aacacgatca
     4381 gcccgagcaa cagcagcggc agccccaggc ccccacgcgt ttcgcgccgg ccgcgccacg
     4441 ccagcacggc aatggtcagc accagcagcc cgagcgtgcc ggcaaagtaa cgatgcacca
     4501 tttccttcca ggctttcgca tgggatacgg ggccgtcggg gcgcacggcc agttcggcgt
     4561 tgatggcagc ggccgcattg tgcggcgtca gcttgccgta gcagccgggc cagtcggggc
     4621 agcccagccc ggcatcggac aaacggacat aagcacccag ggtcaccacg ccgaacgcga
     4681 ggatcagggc tgccaggatg aattttcggt aagtcgtcat catctgcctg ctcaacctat
     4741 ctgggacgcc ttgagcagca gcctgagatc tttcatcatc cgcttgggat cgacctggga
     4801 tggaaaacgc agcataagat tgccgagcgg gtcgaccagg taaatgtgcg cggcccgatc
     4861 ctgcgcggcg ggaaactgcg tcacaaaaga cgcgtccgcc ggccgccaga catgcaatcc
     4921 ggggtgcgcc tgcagaagct gcgcgtcagg cgggccgccg tcagtcatca cccacagccg
     4981 ctcgatccta tcttgctcct ttccctgcgc gatgcgcgtc tggcgcatca ggtagagctg
     5041 ccatgcacaa gccgcatcgc agcgggacgg gcccacatgc accatcaccc atttgccgtg
     5101 cagcgcatcc agattgaacg gacggccgtc ggaactattt cctgctgtct gctgcagggg
     5161 ggtcaccttg agcaggtcgc cgtaattgga gtggccggcc gggcgccacc ccgagtaggc
     5221 cagataggcg gccaccacgg gcatcaggaa cacgccaatc aacagcagga atttactgcg
     5281 cgaagtcaat tgcatacttg aaaccattcc agacgttggg tacatcacgc gtcatcaccc
     5341 cgatgacgtt tcacattcat gaccagccac agcaccgcca gcgtcgtcgc cagactgtac
     5401 cactggatct cgtaacttac atgcatgctg acgccggtat cgggacgcgg ccagtcacgc
     5461 tgcaggccat ccgcctgcgg gctcgtctgc agcagcagca ccggttgcaa cgtgagccgg
     5521 ctggttgcgg cgtagcgccc gaaatccagg ttctgccaca cacgcccctg cggtggggca
     5581 tgcatcaact cgagataacg gctatgcgga tcgactgcca tgccttcgag ctgcaccaga
     5641 ccgcgcggcg tcggcggcag cggaatctgc tcgcgtgagc gcccgaccgc cacccagcca
     5701 cgattgacca gaatggcgag cgacccgcca tctatgatga gcggggtcaa tacgtgatac
     5761 cccgccacgc ccttgtatat ccggttgtcg aggaggatcg tgtgcgcagc gtcaaacacg
     5821 ccgcgcactt ccagcttgcg gtagagcacg ctgtcgcggt ccagcagcgt gctgccgaca
     5881 tggatgggcg cctcccgcat ggccacatca taacgggcct gcagcgcgcg tttggtttcg
     5941 gcacgcccgc tctgccagtt gccgagccac agggtcagca caaagaaaaa cagcgccgcc
     6001 agggtcggcc acacacccgg ggagaactgc catgcgccgg cacgcaactt caatatgtgc
     6061 atggcaaagc caatccggtt agactggaat ttttgcgagc gtaccgttcc atgcgcatca
     6121 ttgccctgct cttcatcgtc ttcattctgg ccagcctcgc cagcgcactc tactatctca
     6181 tcaaggacaa ggggcagtcg gaccgc
//
LOCUS       DAIANL010000084        12208 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_917855, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000084 DAIANL010000000
VERSION     DAIANL010000084.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 12208)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12208)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12208
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917855"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2151 a         3464 c         4092 g         2501 t
ORIGIN      
        1 gttcaggatt ggccgcggcc aatcctgaac agtcacacag attccggccg gagcgcaaga
       61 cgcccggccg gactggctga tcagccggcg atgacgcgaa ccatttccag caccttgcag
      121 gaatagcccc attcgttgtc ataccaggac actaccttga cgaaggtgcc gtccagggcg
      181 atgccggctt cggcgtcgaa ggtcgaggtg cagctttcgc cacggaagtc ggtcgagacg
      241 accttttcgg tggtgtagcc gagcacgccc ttcatttcac catcagcggc agctttcatg
      301 gcattgcaga tctgctcgta ggtggcttcg gtgttgagtt cgacggtgag gtcgaccacg
      361 gacacatccg aagtcggcac gcggaacgac atgccggtga gtttcttgtt cagttccggg
      421 atcaccttgc ccacggcctt ggctgcaccg gtgctgcttg gaatgatgtt ttccagaatg
      481 ccgcggccgc cgcgccagtc cttgttggac gggccgtcca cggtcttctg cgttgctgtg
      541 gccgcatgta cggtggtcat cagcccacgc ttgatgccga aggcgtcgtt cagtaccttg
      601 gccaccggag ccagacagtt ggtggtgcag gaggcgttgg acacgatatc ctggccggca
      661 taggtcttgt ggttgacgcc atagacgaac atcggagtgt cgtccttgga cggtgcggac
      721 atgatgacct ttttcgcacc cgcggtgatg tgcgtctggc aggtttcctt ggtcaggaac
      781 agaccggtgg attcgacgac gatgtcggca ccgacgtcgc cccaggccag ttcggccggg
      841 ttcttgatgg cggtcaggcg gatgcgtttg ccattgacga tcagggtgtt gccgtcgacg
      901 gcgatgtcgc ccctgaacag gccgtgtacc gaatcgtgcc gcagcatgta ggcaaggtaa
      961 gcaggttcga gcaggtcatt gatggcgacg atttcgatgt cggagaaatc cttgatcgcg
     1021 gcgcggaaga ccatgcggcc gatgcggccg aatccattga tgccaacttt gatagtcata
     1081 gtgctagtac tccatgcaag gtagaaaact gttttggtgg caagccggac agacacgaac
     1141 gccgtggcgc aagccgcttc cgttcgggaa gctggccgcg ccttccaggc attgcggcgc
     1201 cggcacccgg ctggcggcgc tgtgccgcca gcctttgccc cggttattta cagtacgccc
     1261 ttgacagcgt ttaccacgtt gtcaacggtg aagccgaagt gcttgaacag gtcgccagcc
     1321 ggcgcggatt cgccaaacgt atccatgcca accactgcgc cttcgagacc cacgtacttg
     1381 tgccagaaac cggtgacgcc ggcctcgatg gctacacgca cgataccctt cggcagcacg
     1441 ctttcgcgcc atgcagcatc ctggcggtcg aacaccgtgg tcgacggcat ggagaccacg
     1501 cgggtggcga tgccctgttc ggccagggct gcctgtgcct tgacggcaag ttcgatttcc
     1561 gagccggtgg caatcagtac agcgcggaag ccatccatgt ccttgagtac gtaaccaccg
     1621 cgttcgatct ggccgagggt ggcttcactg cgcgtcatga acggcaggtt ttgccggctg
     1681 agaatcaggc aggtcgggcc attgaggcgg ctgacgccgt ggccccaggc caccatggtt
     1741 tccacggtgt cgcacggacg ccagacatcc atgttcggga tcatgcgcaa ggttgcggtc
     1801 tgctcgacag gttggtgcgt cggaccgtct tcgcccagac cgatcgaatc atgggtaaac
     1861 acatagatca cccgctggtg catcagtgcc gacatgcgca gcgcattgcg agcatattcg
     1921 gagaacatga ggaaagtgcc gccgaacggc aggatgccgc cgtgcagtgc catgccgttc
     1981 atgatgtgcg acatgccgaa ttcgcgcacg ccataggaaa tgtagttgcc ggtggtcttg
     2041 ccgtgtaccg gatggcaacc ggtccagttg gtcaggttgg aaccggtcag gtcggccgaa
     2101 ccgccgacga attccggcag cgccggtacg agtgcgttga tggcaagctg cgatgccttg
     2161 cgcgtggcca cggtttcgcc tttggcgttg gtggcggcaa tcgcgttgcg caggaacgat
     2221 tcccagttgg ccggcagttc gccttccatg cggcgagtga actctgcggc ctcttccgga
     2281 taggcttcgg cataggcgga gaatttgttg ttccacagtt cttccagacc ctcgcccttg
     2341 acgcgcgcgt cccagccttc gtagacatcg gcagggatct cgaacggcgg atgattccag
     2401 ccgatattgg cgcgggtgtc tgcgacttcg gctgcgccga gcgcggcacc gtgcacatcg
     2461 tgcgtgcctt ccttgttggg cgaacccttg ccgatgatgg tcttgcagca gagcaggctc
     2521 ggtctgtcgg ttacggcctt ggcctgttgc agtgcggctt ccacggcgac ggcgtcatgg
     2581 ccatccacgc cggaaatcac gtgccagccg taggcttcga accgcttggg cgtgttgtcg
     2641 gtgaaccagc cttcgacgtg gccgtcgatc gagatgccgt tgtcgtccca gaaggcgatc
     2701 agtttgccca ggccccaggt gccggcgagc gcacaggctt catgcgaaat gccttccatc
     2761 aggcaaccgt cgccaaggaa cacgtaggta tggtgattga cgatttcgtg accgggccga
     2821 ttgaattgcg cggccagcag tttttcggcc atggccatgc cgacggcgtt ggtgatgccc
     2881 tgtcccagcg gaccggtcgt ggtttccacg ccgggcgtat agccgtattc cgggtggccg
     2941 ggggtcttgg aatgcagttg acggaaacgc ttgatttcct cgatcggcag gtcgtagccg
     3001 gtcaggtgca gcagtgcgta aatcagcatc gaaccgtggc cgttggacag aacgaagcga
     3061 tcacggtcgg cccatttcgg gttggcggga ttgtggcgca agtgatggtt ccacaggact
     3121 tcggcgattt cagccatgcc catgggtgcg ccagggtggc cggatttggc ctgttccacg
     3181 gcatccattg ccaacgcgcg gatcgcgttt gccaggtcgt tacgagttgc catcgtgtat
     3241 ttccttggag gtatgtaaat cgtttacgcc cgggttcccg tccgggccag accgcatttc
     3301 atttgcgcgg tcgaattgtc ggattatcgc tgacccgtcc ggtttcggca agtccgtcgc
     3361 ggctgcatgc gtcggcccgg gaacgcccag tgccctgttt tcgcatgcga ttatgccgga
     3421 ttgattttcc ttgcgacttc gatccaggtg caccagtccg tatagagctc gctgtccagt
     3481 gcggctacga ccgaacgttg ccatggatcg tcggcccaga aatcttcctg ttcgagcgtg
     3541 cggaaggcgc caccggcttc ttgcaggatc aggctgccgg ccgcgtaatc ccacagcttc
     3601 tggccaccgt ggaggtagag gtcgagccgg cctgcggcaa cgtagcacca gtccagcgtg
     3661 ctggcgccga aattgcgctg cgaacagtag ggcggataca gcgagacttg cctgagcaac
     3721 ggcatcggca ggcgtttcat ttcgatgccg gccatggtct gttgcaattc cttgtgtttc
     3781 cgggcgatgg gtagcggcgt accgttgagc catgcgccac ggccgagttc ggcgctgaac
     3841 atttcgtcag cgatcggatc gtagaccacg ccgatttcgc tgcggccgtt gcgcatcagc
     3901 gccaccgaaa ccgcaaaata cgggatgccg ttgacgaaat tggacgtgcc gtcgatgggg
     3961 tcgatgcacc acatcccggc ctgtccggcc agccattgtt cgcggtgctc gtcatcgctc
     4021 atttcttcgc cgaccacggg caacggcgcc aaagcacgca atgcctcgac cagtgcattc
     4081 tggcaggcca ggtcggcttc ggtgaacagg ctgccgtccg gcttgcggct gtgggcgaca
     4141 ttgaggtaac gcggcgtgac ctcggtctgc gcgatatcct tgacgatggt acggatgcgg
     4201 tcgagcatgt cgggtggatt caattctcgt gtttccattt gatggagcag cccatgccgg
     4261 gaatctggtc gagcggtccc tgctgggtga cggcgacgtg taccatggct tcaaacaggt
     4321 cgcggcgggc attgtcgggt gccgtttcct tgcgcgaggc gtcgaagcgg ccacggtact
     4381 gcaactggcc ggcgcggttg agaccaaaga aatccggcgt gcagatggcg tcccacgcgc
     4441 gtgcgaccgc ctgggtctcg tcgaaaaggt acggaaacgg aaaggccagc cgttcggcga
     4501 tgctgatcat gttgtcgaac gaatcctctg cgtattcggc gggatcgttg gacatgatgg
     4561 cgacgctggg aatgccgtgc aggtcacgca attcggtaca gtcgcggatc agccggggca
     4621 gaatggcctg cacgtaaggg cagtgattgc agatgaacat cacaagcagt ccgttctcgc
     4681 ccatgcatga ctgcagtgta tggcggcgtc cgtccacgcc ggcaaggtcg aaatcgggag
     4741 cctgccagcc gaactggcat accggtgttt gcaggcttac catgtaaaac gctccgaaaa
     4801 tgagaatgat ttgggtaaga tgtccatcat accacggtac tccggatttt ttttccaagc
     4861 ccatgcagcg tttttttgtc gaacatctcc ctgatgcaac agcggaattt gaattgccgg
     4921 acgctgtggc gcaccatgcc gcgcgcgtgt tgcgcctgac gaacggcgaa gaaatcaccc
     4981 tgttcgacgg caacggtcgc gaagcggtgg cgcgtatcgt ccgcatcgac cgccgcacgg
     5041 tcgccgtgca ttgcgaagcc gcccgcgcac tgagccgcga gtcgccgctg cgcatcgtac
     5101 tgttgcaagg catttctgcg gccgaccgca tggactacac cctgcagaag agtgtcgaac
     5161 tcggtgtgag cgaggtctgg ccgctggctt gtgcgcgcag cgtggttcgg ctcgatcccg
     5221 cccgcgaacg caagcgcctc gagcactggc gcaaactcgc tgtttcggcg tgcgagcagt
     5281 gtggtcgcaa tcaggttcct gcggttaccg aagtggctgg catggccgag gcgctggccc
     5341 gcgccgatgg ggccgggaaa tggctgctcg atcccgaggg cacgtcatcc ctgcgcgacc
     5401 agaggccacc gtctggcccc ctgttcctgc ttgccggccc tgaaggcggg ttcactggag
     5461 cagaagaggc gcaggcggtc gaggcaggat ttgtccgact gcgtttcggt ccgcgcgtga
     5521 tgcgtaccga aaccgcggca gtggcggtca tggcggccat gcaggcgttg tggggtgatt
     5581 gctagggacg cggtgatcaa ttccacacga tccgtggtga attgatcact gcatccccaa
     5641 ggacgggaat gggggctgct gcattgtgtt gtctggaccg gagcgcagcc atgggtctgt
     5701 gcgctgacag gttgatacaa acggtacgca tcatattcag cctgcagcgg gttccatcga
     5761 tgctgatgac gtcatgggtc tgaatcaatt gcgcaccgca gtcagggcgt ggccgggcct
     5821 tcacccatac cggcgatgat ctgcacgatg gcaccgaagc ggctgtctgt catgcggatc
     5881 ttgaccgggg cgttatcgta gtcctcggcg agccagatct ccatggcttc gtccccggtt
     5941 tctgccggat ggatcaggtg cagcgtgcgc agttttccca acggagtgtc gagcatttct
     6001 tctccggcca ggtgtacgcg gtaggggttg aatgccttgc cgctggtcac gtgcagttgc
     6061 tcgtcgacgc cagacggtgg gcgcagcgcc agctggaaga tcacgctgag aatgtcctgt
     6121 acgtggtcgg gtacgggttc gctgaactgg cggcctttgt gggtgatggt tgcagtgagt
     6181 cgtccggcat cgatgtggac gtgagtcacc tggtcgggac ggtcgctgcc gcgctggatt
     6241 tcaaagtcgt ccggtatcag gccatccgga ccgatgtggc cgctgctgat ctgttccagg
     6301 cggccaggct ggaacagggc gagcaggccg cgcgcctcgg tgatgctgac cagggtgtag
     6361 cggttggtat cggcttgcca gacgtaactg gcgcggccga gtcgagggcc gttttctccc
     6421 agcagcaggt tgaactggat ttcgtaatgg ctgggtagca gcgtgagccg tgtcgatgct
     6481 gttgttgcgg ttgcggtggg cgaggtggcc gcggctggag acggcgtcgc agcgggtgcg
     6541 ggcgagacag cgggtgcggc aggcatcgct ggggtggccg gcgagctgtt tggccatgga
     6601 atgaccggtg gtggcgccga aggctggtgg gcatgtgcat ggtgtgccgg ttgcgggtgt
     6661 ggtggtgctg cagcagcagg agggcgtggc agtggctgca ggcgggcttc gagcggggga
     6721 ggttgtagca ggtttcctcg caggtttggc agatggaagc cgcccagcag cagggcatgc
     6781 agcagcaggg acaacaggat ggcccagagc agtgcatggc gcgacagtcg caggctgcgg
     6841 cgcgtgccag gccgcagttt tgcggcgttg gctggctgcg cacgcgagcg ggccatgttc
     6901 agtcgtccgg aatcagcaca ctttgttcgg agcgggttgc ggcagacagt tcgacccgac
     6961 cgtgcggatc gatatgcaac cggccctgca ggaaccagcg gatggcctgc ggatagatgc
     7021 ggtgctcggc gcgcagcacg cgcgcggcga ggccggtttc gctgtcatgc gccagtaccg
     7081 gaacggcccc ctggacgatg atggggccat gatcgagggc aggcgtgacg aaatgtaccg
     7141 tgcagccatg aactttcacg ccatcttcca gcgcgcgcgc gtgggtgcgc agcccgggat
     7201 agctcggcag cagcgacgga tggatgttga tcagtcggcc gtggtagtgc tgtacgaagt
     7261 ggccggtgag gatgcgcatg tagcctgcca gcaccaccag atcgggttgc cagacgtcga
     7321 tgcgttgcat catggcggcg tcgaagcttt cgcggtcggg gtgatcggtg tgcggcacag
     7381 cttcggcggg aatgccatgc ttgcgtgcga tggcgagccc gctggcgtcg gcgcggttgc
     7441 tcagcacggc cgctaccggc aggccggctg cgaggatggc ttccagattg gagccacgtc
     7501 ccgaaatcag caccaccagc cgtttgtcgt tcatcccgcg attccacggc tggtgaggaa
     7561 ctcctcgtag ttgccgttga acaggtcggc attgcggtcg tggatttcga tgatccgggt
     7621 tgccagcgaa gagacgaagg aacggtcgtg cgatacgaag atgactgtac ccttgaacaa
     7681 ttcgaccgcc gtgttgatcg attcgatcga ttccatgtca aggtgattgg tgggttcatc
     7741 gagcaccagc acgttggccc ggcccagcat cagtttgccg aacagcatgc gtccctgctc
     7801 gccgcccgag agtacgcgca ccgatttctt gacgtcgtcg ccggaaaaca gcagcttgcc
     7861 gagcacgctg cgtattgcct ggtcatcgtc accggcgccg gcccattgtt tcatccagtc
     7921 gaacaggttg agttcgctgt cgaattcgtc ggaatgatcc tgcgcgtaat agccgatgcg
     7981 ggcgttttcc gaccatttga tcttgccggc cagggatgtg tgttcgccga tcagtgtctt
     8041 gatcagggtt gattttccca cgccgttttc gccgatgatg gcgatgcgtt cgccggcttc
     8101 gacgatgatg tcgagccggt cgaacagcgg actgtcatag ccgatggaca gaccttccac
     8161 ctcgaccacg ttgcggtgca actgtttttc ctgctcgaag cggatgtagg ggtactggcg
     8221 cgacgacggg gtgatgaccg tcatatcctg tttcagcttg tcgatctgct tgaggcgcga
     8281 agtggcctgg cgtgccttgg aagcgttggc cgaaaagcgc gacacgaagg tttgaagttc
     8341 ggcaatcttt tccttggcgc gcgcgttggc ggcctgcatg ccatcgcgtt gctgctgcgc
     8401 ggcagtcatg tattcgtcgt aattgccggg gaagatctgg atcttgccgt agtccaggtc
     8461 cgccatgtgg gtgcagaccg aattgaggaa gtgccggtcg tgcgagatga tgatcatggt
     8521 gcagttacgg ctgtccagca cgtcttcgag ccagcggatg gtattgaggt caaggttgtt
     8581 ggtcggttcg tcgagcagca tgatgtccgg attgccgaac aatacctgcg ggagcagcac
     8641 gcgcagtttc cagcccggtg cgactgcctg catggtgccg ttgtgctgtt cgacgggaat
     8701 gcccaggccg agcagcagtt cgccggcgcg tgcttcggcg gaatagccgt caagttcggc
     8761 gaactgccct tccagctctg cggcacgcat gtaatcgtcc tcgctggcgt cggggttggc
     8821 atagatcgaa tcgcgttcgt gcttgaccgc ccacagttgc gcgtcgccct gcatgaccac
     8881 gtccagtacc acctggtctt cgtagccgaa ctggtcctgc cgcagccagc cgagtttttc
     8941 gcccggatcg atgctgacgt ttccgttggt cggcgccagt tgtccggcaa ggatcttcat
     9001 gaatgtggac ttgccgcagc cgttcgcgcc gatcaggccg tagcggttgc cgtcgccgaa
     9061 cttgacggtg acgttctcga acagcggctt ggcgccgaac tgcatggtaa tgccttgcgt
     9121 tgcaatcaca ataggaaccc ggagcgaatc tgaagaaccc gcattttaca acgaagcggc
     9181 ttgcgctgca gcgatcctcg cagtgcttgc gtcggaacag cggtgtggca tcatgtacgc
     9241 ttcgtgattt atgtcgggac tgttcatgga tacgcaagac ctcaagaccc gttttcgcca
     9301 gatggcgcag ggcgcgctga gcctgaatgt ggcattcatc ggcgtggtca acggattgtt
     9361 tgatgcgctg cacgcgtccg cgccgatgac gtccgatgtg ctggcgcaac ggacggggat
     9421 ggatgcaggg tatgtgttgc gctggtgcga cgcagcatat gcgttcggtt acctcgacgg
     9481 tgacaacgga caattccacc tcactgcaac cggccaggcg atgcggccgg gcgcgccgga
     9541 cacgttgatg ccggtagcgg tgcagtcggt gctgtccgcg cacatggccg agcgtgcggc
     9601 cgggttgatg cgtaccggcg agcggccggg ggaatccgcg cttgccgaac gcgcgacggt
     9661 gctaccgtgg ttcggaccga tgctggaggc cagttttgcc gggttgttcg agcagaccat
     9721 ctgcccggcg gtgccagtgt tcgccgccgt ggacgaacgt ggcgggctgg cggtcgatct
     9781 cggctgcggc aatggctggt atctgcgtgc gctggtgcgg cgctgtggcc gcctgcgcgg
     9841 gctgggtatc gatggtttcg cggagaatgt cgatcatgcc cgccggttgg cgcaggagca
     9901 ggggttgtcc gaccggctgg atttcgtgca tggcgacatt caccagatgg tgcttgagga
     9961 gccggccgat ctgatcgcga tgaaccgcgc cctgcatcat gtgtgggaga aggatgcgcc
    10021 gacactgttc cgctggctgc acgataacct gaaaccgggg ggctatgccg tgatctggga
    10081 accggcctgg cccgatgcgc gggccgtgct gcgcgaaccc ggacggccag gcatggcgtt
    10141 ccagaacctg agcgagcacg tgcaaggcaa ccattttttg cggccggatg aaatcgcgca
    10201 cgcatgctcc gaagccggca tgtctgccag cgtatatctg ttcgccaatg gcgcggaggc
    10261 ggtggtggtg gcgcaacggg cgctgtagcg gcgtggcgcg ctcagccgtc gagtctgggc
    10321 cagatggccg ccatcgacag gccgagaatg tacagccagc cgaacaggcg gttgtgtgtc
    10381 aggcaggcgc ggtgatacca cagcggccag ccgatgtagc cgcgcggcgc ggccgcaggg
    10441 cgtgtgctgc tcatcaggtg aatggcgtga atggcccggg gcagggcgat gaaggtcagg
    10501 acggtgaacg ggctcagacg atgcgcgaga atcaggctgg cgatcagcag atacatcaat
    10561 gcgattgctg cgatattgag cgtccgcgcg gcgtggtcgc ctaacagtac cggcagcgta
    10621 cgaatgccca tcgatgcgtc gaaaccggcc tgatcgatgt gcttgccgat caggatggac
    10681 atgacgccga ggccataggg cagcgatgca agcaatgcgg tggatgacag tgtgccggta
    10741 atcatggcgt agccgccacc aatcatcact ggcccccaca ccagcagcac ggcgaattcg
    10801 cccaggccga tacgcttgag cgagcgcggt acggcatcgt accagaacat cagcccggtt
    10861 cctgccacaa aaaacgccac tgccagccag ccgcgctcgg cgatgaaggc acccgccagt
    10921 gcaagaccca gcacggccag taccagcagg ccggtaagca gggtgcgtat ttccagtacg
    10981 ccgctggcca gtggatgcac cgtatagtgc atgcgcggcg aatccggcac atcgtgtccg
    11041 tatttgtagc cgaaatagtc gttgctcaga ttgctgatca tgtgcgccac gatgaacccg
    11101 gcgaatatcc cggtaaattc gatccagtgg aaacggtggt cggcgatggc gagcaggccg
    11161 gcaatgatgg cggcctgagc cgagatgatc aggatgacgc tgcgggcagc ataaagaaac
    11221 ctggataccg ggtccagttc gcggatttct tccacctgag gccggtaaaa gccggtaaag
    11281 gccccgagcc agagcgtgag tctggacggg tggtttcctg aaatcattga tctgctttcg
    11341 ttatcatggt ttgaacggcc gcctgccctg cagcggccac ccgttccatc ggcccgggcg
    11401 gatggtttgg ggtacgctat caaagcagga atcggtctgt caatgcagca ggaatggtca
    11461 tttgaggcca gtctacccca gcggtttacc agtcgccggt gttgggcatg gatagccagg
    11521 gctcggcagg cggcagcggt tcgccgcgct gcagcagttc gatggaaatc tggtctggcg
    11581 aacgcacgaa tgccatgcgg ccatcgcgcg gagggcgggc aatgactacg ccgtgccgca
    11641 tcagccgttc gcatgctgca tagatgtcgt cgacttcata ggccagatgg ccgaagttcc
    11701 ggccgccgcc ataggtttcg gcttcccagt tccatgtcag ttcgacttgc gcctcatggt
    11761 cgcccggggc ggccagaaag atcagcgtaa acttgccctg cggatagtca tggcgacgca
    11821 cttcttccag accaagtgca ttgcagtaaa agtcgagcga tggttccaga tggctgatgc
    11881 gcaccatggt atgcagatat ttcatcaaat gatcctcggg attggggtca gggtgaatgc
    11941 gcgccgacca gcatgggctg cagcttctgc cacacgttgt ccagcacgcg gtactccgct
    12001 tgcgccacgg ggtggaggcc atcggcctgg aactggtcgg cgcgggtttc gatgccgtcg
    12061 agcagcgagg gcagcagcgg cacgcgccgt tgctgcgcga gatcggcgta ggtgagctgc
    12121 aactttttgt tccagctcgt gccataattg ggcggcaggc ggatgccgac caggagcgcc
    12181 cgcgcatgcg ccgcgcggac gagatcga
//
LOCUS       DAIANL010000085         7495 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1330383, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000085 DAIANL010000000
VERSION     DAIANL010000085.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330383"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1599 a         2450 c         2100 g         1346 t
ORIGIN      
        1 tgatactcat ccatcaacac atagcggaac gtcttgcgcc aacgttcgag catctcgggc
       61 caggcggaaa accattcgac cggcaaacgg atcaggtcat cgaaatcaaa tgcctgatag
      121 gcgcgcaggc tgtcctggta tgcctggtac agccgggcat cgtgggcatc ggtttcatct
      181 taggcggcgg caagcgctgc cgaaggcccg agcatggcgt ttttccactg gctgatgcgc
      241 ccctgcaccc ggcgcagcga ctgccggtcg gtatgctgca tcaacccggc cagcacctgc
      301 agcgcatcgg ccgaatcgag caccgagaac ccgggcttgt aaccggcaac tgcggcttca
      361 cgccgcaaga tctgcaaccc aagcgagtgg aaggtgctca gggtcaaccc gcccagcggc
      421 cggcccggca acagtttcgc catgcgactg cgcatttcct gcgccgcctt gttggtgaag
      481 gtaatggcgg cgatgtgctg cggagcatag ccgcaggcat cgatcagcca ggcaatcttg
      541 cgcgtaatga cgcgtgtctt gcccgaaccg gcgccggcga gtaccagcag cggcccgtcc
      601 aggtaacgca ccgcttcgtt ctgtgcggca ttgagcagat cagacacagg aattcgccat
      661 acgacaaaaa gacagggaac aggcaccgct gcggccacaa ccgtcttgcg cttgcccgag
      721 ccggggttcg cacgaatgca acacgcccga aatacaattg cgacgattgc atcaatcgcc
      781 cggcattcta ccgtaaaatg acactgtccc ggcagacgga ccggattcgc ggccgctgca
      841 ccctcacact ggaacccggc aacatgtatc cgcttcccgc ttgccttcca ccggaaggcc
      901 ccagcccatg agccattatg caatcggcga tctgcaaggc tgttatgccg cgctgcaacg
      961 catgctcgaa cgcattgatt accgccccga tgaagaccgc atcttcctcc ttggggatct
     1021 ggtcaatcgc ggcgaagact cgctgtcggt gctgcgatgg gcacgcagca acggcatcgg
     1081 cgccattctc ggcaatcacg acctgtacct gttgtcggtg gccgaagggt tcgccaccag
     1141 ccgcgaggat gatacgctgc acgagatcct tacggcgccg gatcgcgacg aactgttaca
     1201 ctggctgcgc catttgccgc ttgcgcagca cgtgcaagga tacctgctga ttcatgccgg
     1261 cctgctgcca cagtggagcg tcgccgaggc gcttgcactg gcctcggaag tggaaaccgt
     1321 gttgcgcagc gcccactacc acacattctt gcgcgacatg tatggcaacc agccggccgg
     1381 ttggcacgac gatctgcgag gcgcagaccg gttgcgcgtc atcaccaatg cgctgacgcg
     1441 gctgcgcttc tgttcgacca ccggcgacat ggacttcagc tccaaaggtg atccggcaca
     1501 cccgccacca ggctgcctgc cctggttcca gatacccaac cgccgcagcg ctggacaccc
     1561 gatcgtgttc ggtcactggt cggcgctggg attacagttg catacagaca gcatcgcact
     1621 cgacaccggc tgtctctggg gcgggcaact cacggcattg cgtctggaag accggcggat
     1681 atatcaggtg gattgcgcag aacttcctgg gacaaggccg ctgcgatagc ctgttcactg
     1741 ctctgcgcca gttcacgccg gctgcgtccg gacgctgcaa ccgccggggt gaacatcagg
     1801 tcaacgtcca gccggcacag accgagcatg cgccacaacg attcgcccag ccccatgtca
     1861 tcgatccact ccgcgcctcg ctgccgataa cggatgccca ccggcatgca caacgcatcg
     1921 cggctgaccg ccacctggaa cagggccgtc ttgaacggac gcagaaacct gccattgccg
     1981 gtggtcgatt cgggaaacag cgccaccact tcgccggcat cgatccgcgc ttcgaacagc
     2041 cgggtcacgc gcgcgacgtc ccgtctcagg tcgcgacgga tgaataccgt accggcacga
     2101 accgcaatcc agccgaatac cggccagctg cggatctcgt ccttggccac gaaacgcacg
     2161 ggaaaacacg ccagcaacag aaacgggtcg agccacgaaa catggttggc caccaccaga
     2221 ccgctgcctc gtggcatctc gccggacaga tgcagacgta caccaaacac cgccagcagc
     2281 ccgcgcgacc agacactggc aatgcgaccg cgcaaacgca acgatatcag cggaaatacc
     2341 agcgcgaggg tcgcgaggcc gccaccaagc cagaccagca gccagacggc gcggaacggg
     2401 cgcaatagcg ggcaacgatt caacggttca gcagacatgc accgattgta ccccggataa
     2461 gcgggtcttc gtccggccac atcactcctt ggtcatgaaa tgccgggcat attgccggtt
     2521 catttccttg accgacagga gcatcatgaa atcggcacag ttgaattcgg catcccaggt
     2581 gggttcgcca caaatgaacg caccaacacg caaatagcct ttgataagcg gcggaaccgg
     2641 tacggacgcg tcattaccca gtcggtcgag tgtcagcgga acccgaggaa agacccgcca
     2701 ttcgaccgga ctcaacacct cggccgacag cttgcggtac agattggccg ccgtatgacc
     2761 accatcctgc ataccgacac tggcgcagcc gaccaggtaa tcgtaatcgt gtttgcgaag
     2821 atagtcggca agaccggacc acaacagcgt gatcaccgcg ccgccgcggt agtcggcatg
     2881 cacgcacgaa cgaccaattt caaccatgcg cggacgcagg tgcgtcaacc gggtcatgtc
     2941 gaattcctgt tccgaatagt aaccgccgat cctggctgcg ctttccgggg acagtacacg
     3001 ataagtgccg acaacattgc cgctgcgctg gtcgcgcaca atcagatgat cgcataccgg
     3061 atcaaactca tcctggtcaa gcccgcttga agctgaaaca agctgcgctc ccatttcttc
     3121 tgcaaaaacc ttgtaacgca aacgttgcgc ctcgcgcact tccgccgggt cgcgagcaag
     3181 ggataccgtc aggctgcttg cccgggaacg cggttgagaa acggtctgct gttcgtacat
     3241 catgaactcc tggacacggt gtcgatgcgc taccttactc aatccaggtg acgttcatgt
     3301 gaaaaataca tgacatatca aagacaaatt tgatacatgc cgtacgggca gtcaggttgc
     3361 tgcaatactg cctgcactgc cgaccgatcc ctgcggcgca accgacgacc cgttcagaca
     3421 tcctcttcgt tacccggatt atccgtctga cttgtcgaac gcgttttgcg cgcagccttg
     3481 tcgcgtatcg aacgcaaggc ctgaatcgta ccggtgatcg gcgccagttc cgggttggca
     3541 gctgccatgg cacgcaaggc gcgattgctc gtcgcaccgg tggccatgcc acctcccgcc
     3601 ccggccagtt ttccctcgag gtgtttgcgc ccgtgcgctt ccatcgattg cacgacgtcc
     3661 ggtttcatgg cagacaggct ctgaatgtgc agcgacatgc cgctcgccag ggcatgagag
     3721 aacgtcggga tctgcttgct cagcgaatag gccatgaaac tccagaacag catgatgatg
     3781 ccatagacga tgaaaccgag aatgttcttg ccaatttccc acagccagcc gctgtccagc
     3841 ccaaccacat tgccggcata ctgatagatg atgttgaata ccggcaacat cagcgccgag
     3901 agcaccaccg caatcagctt gtagatgccg ccagaaatgg cgaattcgag ccatttggaa
     3961 aaatagtggt tggcgggtgg aaaggcgagc gttgcgacga acactggtcc caccgccaga
     4021 gcgatgtaga tgatgaaatc gcccatattg atgaacagca gcgaatacag catggccacc
     4081 acgagcgtgc cagcgcagat cagcaccacc cacaccacga gcacgacata aagcaacacc
     4141 ataccccagg caaacagctc cggcaccacg atcccctgca ccgacagctg tgccacgaac
     4201 ttggcgcgtg acagccagct accgtggtcc cacatctgtg gaatcacttc gagcagatcg
     4261 acgatggcgt tccagtaatt atcgaccatc tggtcggtgg gaccggaacc cgcaccgaac
     4321 ggctgactga gctgctccat gcccacggtg agaaaaccgg ccacacccac cccggcggta
     4381 tcagtccagc ccagcaggat ctccaacgca atcagggtga ccaggatctg gttgatcagc
     4441 gtgcggatca cctgatccat ctgccccgaa aggataccct gaaaaatatt ccaccccgca
     4501 gtgagggcaa aaaataccgc cagcagtttc tttccggtcg gcaccagcaa tggtgccagt
     4561 ttcgcccctg ccacatgcat ggcgccgatt accgcgacca cggattgctt gaaggattgc
     4621 tggccattga gcaccatatc attgccggtt gtggccgccg gcgcggacgt tgcggcctgc
     4681 gcagacacac tgcaaccggc agccgccagc atcagcacca gccacaacaa ggtacgcacg
     4741 cagaatttca tctcatcctc tccagcgagc cagtatgcgt gccagggtca ggcacagaaa
     4801 cagcaggttg ctgaacgcga accagatgaa cagcgtcgga cgatccacca cctgcagcaa
     4861 caacccgagc gcgcacattt gtcgcacatt gcgcgccacc cgccgcgaca tgcggtaatc
     4921 gcgtcgtccc gctggcggca gcaggcgaaa ccgtaccgct tcgcgcagca cacgatatcg
     4981 ttcagccggc gcaagccaca ggcgcgcgat tcgccacatc aaacgcatca tggaccgccg
     5041 gccgtgccga aatttcgcca cgcattctgt tcgatctgct gctggctgta gcccgaaggc
     5101 atggcgttct gcgcgtcaag acgctgcctg gccgcctgaa tctgcgcctc ctgccgggca
     5161 atgtcctgct gcgcttgggc aaggcctgcc tgctgttgct ggctgtcgtt catctgccgg
     5221 gcgtgcgtct cggcgctggc cgccgcaacc tgcctgttga gggtcagggt ctcgcccgcc
     5281 accacgctga ccagcgcgtt gagcttgtcc agcccctcga ccgccccggt actcggcagg
     5341 ttgtcgctga tgttttgcag cgccgacgaa cgcgttacca catcctgcag ggtttgcgtg
     5401 tcccgctggt aggctgccgc atagacacca ccacgggtgt gcgccagttg tgcctcgaac
     5461 tgcaggtatt gctgcggagt ggtgccgagc gcctgggctt cggccaggcg cgcctgcacc
     5521 atctggcccg cctgctgaaa cgtggtctgg gccgcctgca cggcatcgag cagctgctgc
     5581 gcctgcagcg cctgattctg ataaggcgca acggtctgcg tcagcacggc ctgaggagca
     5641 ttggccagat tgcgcgcagc cgcctgcagc tcctgcaccc gcaccagttg cgcctgaatc
     5701 tgattggcca cgctttctgc ctgtttggcc gagttgtaca gcagctcgcc ataattgagc
     5761 agctgcgtgg gttcggtggc gcccccgacc atcagggtca ccatgccacc cgcccggcag
     5821 ggcacagcgc acagtaccgc gctacacagg cagacggcca gccaggcact ccgcgaccgg
     5881 cgcaagcaga ttttcatggc aactcccttt tccccgcatt tgatgctgcg tcgcacatgt
     5941 catttcgaac gctgcagcat ttcctcaaca tagcgctgac gccaatccgg cttgcccgac
     6001 gccgcatggc gttcgaatac cgcctgtgcc agcgcatccg aacgcacgat cgccagcgtt
     6061 tccgcgtcga gcggcagctc cgcacgccga aatacgccgg gctgctgtat aaaataatca
     6121 cgcttgggca ccccggctgc gatctggcgg atctggtcgt cctgcaggcc aaactgatcg
     6181 cggtacaacg gcagcaattg cggcgcatct gcgttcatgt tcggtaaata gatgcgcgtc
     6241 tggatgttgt cgcgcaggct ggaaaacaca ttcgaaccgg caatgtcctc caacgattgc
     6301 gtggccatca ccaattgcgc cacaagcttg gcgaaagttt tggtccagtc gcggattttc
     6361 tgttcgaacc aggggtgctc aagcatgaac cagcattcct cgacatagat cagcgtcggt
     6421 acgccctgcc gcccaccctc gcgcaattgc aggtagatgc ggtagaacgc gtaatccatg
     6481 aaggcgcgcg ccacctgctg ccgctgcagc atctcgccca tttcgatgca gcagaaatcc
     6541 gtcagcgaaa acgtgtcttc acggttgtcg aaataatgcg ccagcggccc ctgtccgacc
     6601 caggcatcga gttcattctg caacaccggc gacaggatcg aatgcaggtg gccaagcgtc
     6661 cagtgggcgc gatcctgtgc tgccaggccc tgcaagccgc gccatacgtc cttgacatcg
     6721 tccgcgctgt aacggtaacc gcgatggcac accaggccct ccacccatcc ggccagccat
     6781 tcgagcgccg cagcatcacc cagcagcgca agcggattcc agcggctgcc atctcgcaca
     6841 ccgatatcga tatggtcgcc gcccatcatg cgcgttgcaa tacggcagga gcgatccttg
     6901 tcaaatatga tgacccgaca gggtgcgtat tttctgaact gcgcaatcaa tacattcatc
     6961 gcgctggatt tgcccgaccg tgacggacca acaacgagcg catggccaag atcattcgaa
     7021 tgcagattga agaaatacgg cgtattgtgt ccggtctcca gcatcatcag tgccggagaa
     7081 tacttcccgc gctgctgggt cagatactcg ttttcgatcc ggccgggatc cacagtacgc
     7141 agtggcgtca gatcggacag cgaacgaatg tcgagcagga accagcgcct gatcatggta
     7201 tgcatgccag ggataccgcc ggcaaaggcc gacagcaggt ggatttgttc gcggatcact
     7261 gtaagaccgg cggcttccat gagcgccgtg atgtcatcca gcacccgacc cgcttcttcc
     7321 gcacccgcgc ccagagcgaa cacctgcgcc gaataccagc cataccagac ctcgtcagtc
     7381 tcgacccggg tacttgcaag gtgggccgcc tcggccgcct ccagccgcga ctcattggcg
     7441 cgcgcatcgg cctgacggtt catcgccaga tacagccagg tcatgggcga gtatt
//
LOCUS       DAIANL010000086        12286 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1736570, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000086 DAIANL010000000
VERSION     DAIANL010000086.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 12286)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12286)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12286
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736570"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2413 a         4188 c         3597 g         2088 t
ORIGIN      
        1 catccggcgc atcgtcgcgg tagagaaaca ccatgtccag atcggaggcg taaccgagtt
       61 cgcgtccgcc cagcttgcca taaccgatga tggcaaaatc cggggtctgg cggtggcgct
      121 ggcgcacgcc ggcccaggca agcggcagca ccgcctgcag gaccagttct gccagccgcg
      181 tcagataccc ggccagcgtc ggcagttcca gccggccgga cacgtcctgg gccagccagt
      241 ggaaggtgtg ctgctgacga aaacggcgca ggatgtccat ctgccgctcg gtgtcctgcg
      301 ctgcatcggt cagccgcatc gccagcatgt cgcgcatggc cttccagtcc ggcacttcgt
      361 cgagcaggcg gtcatcgatg agttcgtcga gcaggatcgg attgcgtgcc agatactgcg
      421 ccgcccattc actggctgca cacaggtggg ccacgcgttg caacgtggcc gggaactcct
      481 ccatcagcgc caggtaggcc gcgcgaccgg cagtggcctc gagcagatcc atcatgcgcg
      541 ccagcgtggt gtccggcggc ggaaaacgca ccgcttgctg caacagcact ggcgccaacc
      601 ggtcgaaacg ttcgcggctc gattccggca aacgcagata acccacgctt ctgcgcacct
      661 ggatcaggcg cgcgtacacc gtggccggat cgatataacc gagcgccgcc aactgttcca
      721 cggcatcctg gtcctcgacc agaccgagcc acagagcgcc gagtggatgg atctcgtcct
      781 gttcgccgcc acgggaaaat atctcgccga accagtggct gacccggtcg cggtggctgt
      841 cgagcgccgc catcagactg gcttcgccgg cataacccat ggcggtggca atgcgctgcc
      901 ggtcttccat cgtggccggc aagatttggg tctgcgcgtc atcgaggtat tgcaaccggt
      961 gttcgagatt gcgcaggaac acataggcca gttccagttc ctgcaccgcg gcgggttcga
     1021 gcagaccccg cgcgcccagc accggtagca tgccgagcgt ggaacgcccc tgcaacgccg
     1081 gttcctggcc gccacggatg agctggaaca cctgcacgat gaattcgatt tcgcggatgc
     1141 cgcccggtcc gagcttgata ttgtcactgc gatcgcggcg caccacatcg cgccggattt
     1201 gcgcatgcag cgcacgcagc gcagagatgg cgccgaaatc gaggtatttg cggtagacga
     1261 acggccgcac gatggcaaac agctcatcca gccggctgcc ggtgatcgcc cgcgccttga
     1321 tccacgcata acgttcccat tcgcgcccgt gcgcggtcag gtaatcttcc agcgcagcat
     1381 aacccatcgc cagtggtccc gagtcgcccc acggccgcag gcgggcatcg acgcgaaaca
     1441 cgaaaccatc gccagtcggc gccgccagcg tacgcgccac acgctgcgcc aatgacacga
     1501 aaaactggtg gtgcgacacc ggtcgcgggc catcggtctg cccttcttcg gcatacaggt
     1561 aaatcaggtc gatgtcggaa gagacattga gctcgctgcc gccgagcttg cccatgccga
     1621 cgatggtcat ttcgatgggc atgccggcag tgtcccgggg caggccatga acttcgccca
     1681 gccagcgcgc atggtgtgcc agcgcacagt ccagggcgat ttcggccagc tccgtacagc
     1741 cctgcatgac ttcctgcaat ccgccactgc cagccagatc gcgcgccatc cactgcgcca
     1801 gcatgcgccg acgcagcagc cgcagcgcgc gatccagcgt cgccgcatcc gtgatgggct
     1861 cctgtgccag ccactcgcgc atctgctgcg cttcccaggg ccgcgcgacc tgcccggcaa
     1921 gcgccgcttc gctgatcact cccgactgca acagctgatt gaaccagcgg gagaatttgc
     1981 cggtctcatg caatatcgat tcgccattca tgccatgtcc gcccgccagt ccatccattg
     2041 ccagtttcac cgcgtcccgt ccgtatcgtc cctgctgcgc agcgcgccgc gcggtgcggc
     2101 gcaaattcga ttaaaatagc gcaaaatcac caaagccacc gcctggagtc attcatgtcg
     2161 cacatcgagt ccgtcctgca cgaaaaccgc tcgtttccgc cgcacccgtc attcgaggca
     2221 cgcgccaacg tatccggcgc cgcgcaccac caggcgctgc acgaacaggc ggccaccgat
     2281 ctggacgggt tctggggcga cctggcgcgc aggcatatcg gctggcacac gccgttcacc
     2341 cgtgtgctcg acgaatccga tgcgccattc taccgctggt tcgacgatgg cacgctcaat
     2401 gtttcctgga actgcatcga ccgccatctt gcagaacacg ccgaccgcac cgctctgatc
     2461 tttgaggccg acgacggcgc ggtcacccgc gtgaattacc agcaactgta cgagcgtgtc
     2521 tgcgtgtttg ccaatgccct caagaggcat ggcatccaca agggcgaccg ggtagtgatc
     2581 tacatgccga tgagcatcga ggccgtagtc gccatgcagg cctgcgcacg catcggcgcc
     2641 atccattcgg tggtcttcgg cgggttttcc gccaaatctc tggccgagcg gatcatcgac
     2701 gccggtgccc gtctggtggt gaccgccgac gccagcctgc gcggcggcaa gtcgattccg
     2761 ctcaaagcgg tggtggacga ggcgcttgcc ctgccgggaa ccggatgcat cgaacaggtg
     2821 gtggtctacc ggcgcgccgg cctgccgatc gcatggcatg accagcgcga cacttactgg
     2881 gaagcgttta ccgccgacct gccagcgcag tgcgaaccgg aatgggtcaa cgccgaagac
     2941 ccgctgttca tcctctacac ctccggctcg accggaacgc ccaagggcgt acagcacagc
     3001 agcgccgggt acctgctcgg cgcgatcctg tcgatgcact gggtattcga cgccaaaccg
     3061 gaatcggatg tgttctggtg cacggcagac gtgggctgga tcaccggaca cagttatgtc
     3121 gcatatggcc cgcttgcgct gggcatgacc gaagtgatct tcgaaggcat tcccacctgg
     3181 cccgacgccg ggcgtttctg gcgcatgatc cagaaccatc gtgtgaccac attttacacc
     3241 gcaccgacgg ccatcagctc gctcatcaag ctgggcagca acctgccacg ccagtacgac
     3301 ctgtcgagcc tgcgtctgct gggcaccgtc ggcgagccga tcaatccgga agcatggatc
     3361 tggtttcacg aagaagttgg cggctcgcgc tgccccatcg tcgatacctg gtggcaaacc
     3421 gaaacaggtt cgcacatgat tgcaccactg ccgggcgcgc acgcggccaa gccgggttcc
     3481 tgcaccttgc cgttgccggg catcgtggct gacgtggtcg acgagcaggg ccagtcgctg
     3541 ccagccggcg aaggggggtt cctggtcatc cgtcgcccgt ttccgtcgca actgcgcacg
     3601 ctgtggaaca acccggaacg cttcaaggcg acctatttcc ctgacgaact cggtggcacg
     3661 acctacctcg ccggcgactc ggcgcatcgc gacgaagaag gctatttctg gatcatgggt
     3721 cgcatcgacg atgtgctcaa cgtctcgggc caccgcctgg gcacgatgga aatcgaatcg
     3781 gcgctggcgg cgcaccacag cgtggccgag gccgcagtgg tcggccggcc gcatccgatc
     3841 aagggcgaat ccatcgttgc cttcgtcgtg ctcaaaaaca aacgccccga aggcgaagaa
     3901 gccgcgcgca tttccggcga actgcgcagc tgggtcggcc aggagatcgg cgccatcgcc
     3961 aagccggacg aaatccgttt cggcgagaat ctgcccaaaa cccgttccgg caagatcatg
     4021 cgtcgcctgc tgcgggcgct ggcgcgtggc gccgacatcg accaggacac ctcaacgctc
     4081 gaaaatcctg cgattctcga tcaactgcgt actgccagct gaccgatggc ctccgcgcgc
     4141 cttcagccga tccgccgcat tctgctgtgg ctgaccggcg ttgtcgcgct cgcggcatcg
     4201 agcctggtgc tcgcgttgca attctggatc ctgcccaata tcaaccactg gcgcgccgac
     4261 atcgccgctt cgatcagcgc ttcatcaggc gaacgggtca cgctcggacg catcgacgcc
     4321 ggctggcagg gctggcaccc gtcgctgcgc atccagtcgt tgcagatcgc cgacgcgcag
     4381 cagcaccccc tgctgtacct caccgacatc gaaggcgaaa tgtcgtggtc cacactgctg
     4441 ttcggcgaac tgcggctggc acggctcaca ctgagcaacc aggcgttttc cctgcggcgc
     4501 gaccgcaacg gcacaatctg gctggcaggt ctgccgctca accggccgaa cggaaaccac
     4561 accttcgtgc agtggctgct gcaccagcgt ctgatccagc tcaaccatgc ttcgctatca
     4621 tggcaggacg accagcggcc ggcccccacc ctgttcatcc acgaagtcaa tttcagcctg
     4681 accaacttcg agcgctggca taattttcac ctgtcagcca cgcccccgac actgatcgcc
     4741 catccgatcg acatccaggg cagcctgacc ggacgctccc ccgccgatcc tgcgtcatgg
     4801 agcggcaaga tcagcctgcg cctgcgccgc gccgacctgg cacaatggca accctgggtc
     4861 acactgccac tgggaatcag gcgcggtttc ggcgatgttg acgcctcgct gcaattcgcc
     4921 cgcaaccaga tcatcgaagc cagtgcccgc ggcatcctgc aacaactgtc gctgcaagct
     4981 gcatctgacc tgccccgcct cgacctgatc gacctgtccg gggggattga ctggaaacgg
     5041 ctcggcagcg cgcaatcgct gcagctcgat cacatcagcc tgcgcactgc cgatctggtc
     5101 tatcttgcgc cactggattt ttacctgcgc attgcgccgg cctctgcgca tcaggcaccg
     5161 cagggtgaat tgcgtgcgga caatgctgac ctggcaacgc tcaaccgcct ggggcactac
     5221 ctgccgctgg acacggggca gcgcaactgg cttgcgcagc atgcgccatc tggctgggta
     5281 cagagcctgc gcgccgactg gagcggcgca ctgcctcggc cacagaaatt ccatctgcac
     5341 gcccgattcc gcaccctggg cattgcctcc gccggtggcc agccgggagt ctcggggctg
     5401 agcggacaga tcgacggcga tgaaaccagt ggctcgctca cgctggccag ccagcagaca
     5461 agcgtcgacc tgccggatat cctgttcgaa ccgcacgttg cgctcgatac cctgaccgga
     5521 cagttgcgct ggaagaaatt gtcatccggc taccagctac agctgtcgca gatcaatctg
     5581 gccaatgccg atttcgatgg caatctgttc ggcgaatata cctggctacc tggcaagcga
     5641 ggcctgatca acctgagtgg cggcctgctg cgtggtcgcg gcatcgctgt gtgccactat
     5701 ctgccgcttg ccgtcaagca gccggcctac gactgggtgt gcaccaacct gctcggtggc
     5761 gatgccaagg atgtccagtt ccatgtcgaa ggcgacctgg atcactatcc attccacaat
     5821 gacggtcaag gattgctcga ggtacgcatt ccggtcaaca acgccagcat ccggcccaat
     5881 ccggatttcc cggccatcga ccacatccgg ggcattgtcg aatttgccgg tacgaaaatg
     5941 ctcatccatt cggatagcgc ccggctgtac aacgcacaga tccgcaatgc cagcgccgaa
     6001 atccccgacc tgttcggcgg actgtcggaa agactgctgg tcagcggcca ggccaacggc
     6061 accgccagcg atttcatccg ctttaccaac gaaagcccca tcggcagcac ggtactggat
     6121 cacctcaccg atggcgccag tggcagcggc aagctgcagt tggcgctgca cctgcaactg
     6181 ccattccatg acatgaacca tccgacggtc agtgggcgac tcgaattcaa ccgcaacgat
     6241 ttcattctcg ccaacggttt gccacccatg cgcgcagtac aaggcaaact cgatttcaca
     6301 caaaccggca tcagcgccaa caatatcagc ctgcgcctgt tcggccgcca ggccaccatg
     6361 gacagccaga cgcaaaacgg catcacccga ctcaacgtga aaggtcaact ggtcgccggc
     6421 gatctcgtcc agtggctacc ggccggaatt gcggctcatt tcactggcac gacaccgtgg
     6481 caagccagga tcgacatggt gcgaggccgc atcgcaacca caacgttcga cagcacgctg
     6541 accggactgg acatcgccct gcccgccccg ctggacaaag cggccagcgc acacgtgccg
     6601 ctgcatgtca cgagccggcg caccggcgac gaccgccagt tgatcgacgt cggttacggc
     6661 gccgtgttct cggcgcaact gttgaccgcc gcagcatccg gccccggact gccacccatc
     6721 gaacggggca atatccactt tggcggtcct gcgctgcttc ccaccggcaa caggatcacc
     6781 atcacgggca aggtcgcccg ggccgatctc gatggctggc tggcggccgg aacttccggc
     6841 caggcgggac tgccgctgga cgatatccgg ctcgatatcg gcacagcctc gctgttcgaa
     6901 cgcaatttcg atgccattac gctgcacgcg cacagcagcg acaaccgctg gctgaccagc
     6961 ctgcatgggc cgaccaccca gggcgaactg acctggcagc cgggatcagg caaggacagc
     7021 cgcggtacgc tgcaagcccg cttcaccagc ctcaccatcc catccggcac ggtgaacacg
     7081 gcaaggccat ccggggacgg tgcggcgcac gatgcggcca acgacggtgc caattggccc
     7141 gcgcttgaca tcgatgtgga tgccctgcaa ctgggcagtc gccggatggg gcggctcgaa
     7201 atccgcgcca ctccgattgc cggtggtctg catttcgacc gcatcgcgtt gatccatccg
     7261 gacagcaatc tgaccatgtc agccgactgg accccgcatg ccacaccaca gaccaatgcc
     7321 agcatgcgac tggtggtgaa caatctgggc gatttcctca cccgtttcga tgaagcggac
     7381 accatccacc gcggcaaggc cgtactcgat ggccatgcca gctggaatgg tgctccgctg
     7441 gacattccaa tcgccagcct caacggcgca ttcagactgc aggcagaaaa cgggcagttc
     7501 ctcaagatcg agcccggagc cgcgcgcctg ctgggtgtgc tcagcctgca ggatctgcca
     7561 cgacacatga cgctcgattt tcgtgacgtg ttcagcgatg gctttgcctt cgacgacatt
     7621 tccgcgacgc ttgacctgaa tcacggcata atacacagta atgattttgt catgcagggg
     7681 ccggcggcca ccgtcgaaat gcacggagaa gtcgatctca acgctcagac ccagcgtctg
     7741 tacgccgtag tcagcccgaa actgagtgaa agcgttgcgt tggcaagttc gctcgtcggc
     7801 ggcccgatcg tcgggctcgg tgtgctggcc gtgcagaaac tgctggaaaa tccggtcggg
     7861 catgccatcc gcttcgatta cgccatcagc ggaacgtggt cccacccgaa aatcgacagg
     7921 actggacagg aaaatgagaa aaaatagcct gctgacactg atggtattgt tgctcgcgtc
     7981 accagcgaca tgggccgatc cgctcagctg cacgccgggg gacgatttct ggctcggccc
     8041 gcgctctggc gcatccgttc tggcgaacgc ggcacttgcc ccctgtgtgc acgccttgct
     8101 tgatgacgcg cacacccgtc tggtcatcac ttatcccgac ggcgacgatg ctgcagtccg
     8161 cgcggatgaa ctgcgccagt ggctgattgc gctggccctg ccagcgtcgc gcatcgccct
     8221 gcagcaacag actgccaaca ccacgatcga gctggaaaca cgaaatgact gaacgtaaca
     8281 cccgcaaaaa aacccgtgcc actccgcaac gcagcatctt ccgcgttgcg gccatccaga
     8341 tggcatccgg ccccaatgtc gcagccaatc tcgccgaagc cgagcgactg attgcacgtg
     8401 ccgcaaccca gggcgcacaa ctggttgcct taccggaata tttcgccatc atgggcctgg
     8461 ccgacactga caagctggcg gtgatggaag acgaacatgg cggcccgatt cagaaatttc
     8521 tcgccaacat ggcgcgcaag cacaaaatct ggatcatcgg cgggtccgtg ccgcttgcag
     8581 cctcggcacc caacaaagtg cgcaacagtt gcctggtcta cgacgcacac ggacaccagg
     8641 tagcgcgcta cgacaagatc cacctgtttg gactcaacct cggtaccgaa cactatcagg
     8701 aagaaaacac catcgagccg ggcaacagca tcgtggtatt cgacagcccg ttcggtcgca
     8761 tcggcctgtc gatctgctat gacctgcgct tccccgagct ctatcgtgcc atgaaagatg
     8821 tcgatatcat cgtcatccct tctgcattca ccgccaccac cggcaaggcg cactttgaaa
     8881 cgctggtgcg cgcgcgcgcc atcgaaaacc tggcctatgt catcgctccg gcgcagggcg
     8941 gctaccacct ctctggccgc gaaacccacg gcgacacgat gatcgtcgat ccctggggtg
     9001 tggtgctcga ccacctgccc cgcggctcgg gaattgtcat ggccagcatc aatccggact
     9061 atcaggctca cctgcgcagc agcctgcccg cgctgcggca ccgcacgctc gactgcagcc
     9121 acctggcacc tgcaaaagac agcgcaagca acgccgatcc ggccgcaagc caacccgatg
     9181 ggaccaccac atgagcactc tgagcggaat ctataccccg aacacgggca gcctgctgga
     9241 aatcgcccac agcacgctgc tcatgccgaa ccagatcgag tctccccaac tcgatgaagt
     9301 gttcggccag ctgctgactc accggctgga ctatgccgac ctgtatttcc agtacacgcg
     9361 ttcggagagc tggagtctgg aagaaggcca ggtcaagtcg gccagcttca acatcgatca
     9421 gggcgtcggc gtgcgcgcca ttgccggcga gaaaaccgcg tttgcctatt cggacgacat
     9481 cagccgcggc gccctgcttg ccgctgccca tgccacgcgc gccatcgccc agagcggtgc
     9541 cgaacaccgg caccgcgtcg tcacacacgg tcagcggcgc gaactctatc tgcccactga
     9601 cccgcttacc accctggaag acgcgcagaa agttgcgctg ctcgaacaac tcgaacgcta
     9661 cgcccgtgcc atcgacccgc gcgtgactca ggtcatggcc agcctcaacg ccgaatatga
     9721 cgtggtgttc atcgcccgtt ccgacggtca ccaggcggca gacgtgcgcc cgctggtacg
     9781 cctgtcgcta caggtcatcg tcgaacagga cggacggcgc gaacagggca gcgcgggcgg
     9841 tggtggccgt tccggttacg aatatttcga tgacgcacgc ctgcggctgt acgccgagca
     9901 agcggtgcac caggccatcg tcaatctctc cgccggtccc gcaccggccg gcaccatgac
     9961 cgtggtgctc ggtgccggct ggcccggaat tctgctgcac gaagccatcg gccatggact
    10021 cgaaggcgat ttcaaccgca aaggcagttc ggccttcagt ggacgcatcg gcgaacgcgt
    10081 ggccgccacc ggcgtcaccg tggtcgacga cggcaccatc gcccaccggc gcggttcgct
    10141 caacgtcgac gacgaaggta acccgacgca atgcaccacc ttgatcgaaa acggcattct
    10201 caaaggttac atgcaggaca gcctcaatgc gcgtctgatg ggcgtaccgg tcaccggcaa
    10261 tgcacgtcgc gaatcgtttg cgcacatccc catgccgcgc atgaccaaca cctacatgct
    10321 caacggtgac aaggatccgc gcgaaatcat cgcctcggtc aaacatggcc tgtatgccgt
    10381 caatttcggc ggcggtcagg tcgacatcac cagcggcaag tttgtctttt ctgcggcaga
    10441 agcctatctg atcgaaaatg gcaggatcgg cgcaccggtc aagggcgcca cgctgatcgg
    10501 caacggtccg gacgtgctga cgcgcgtgtc gatgatcggc aacgacatgg cgctcgatcc
    10561 cggcgtcggc acctgcggca aggaaggtca aagcgtgccc gtcggcgttg gccagccgac
    10621 actgcgtatc gacgggctga cggtgggcgg cacgggctga ggcgtcaacg aggcgacatg
    10681 ccctgcatgc aagcgtgggg cgacttgcgg taaaatagcc gtttttcgcc caacactccg
    10741 tcatgatccg cacccgcttc gcccccagcc cgaccggcta cctgcacatc ggcggcgcgc
    10801 gcaccgcgct gtattcctgg gcgtatgccc gccaccacgg tggcacattc atcctgcgcg
    10861 tcgaagacac cgacctcgaa cgctccactc cggagtcggt ccaggccatc ctggacggca
    10921 tgcgctggct cggcctcgac tacgacgaag gcccgttcta ccagatgcag cgcatggacc
    10981 gctaccgcga ggtgatcgcc caactgctgg ccagcggtca tgcctaccat tgctatgcca
    11041 gtcgcgagga actcgatctg gtgcgcgaac agcagcgcat cgccgggctc aagccccgct
    11101 atgacgggcg ctggcgcccc gaacccggca aggtactgcc gccgccaccc gcaaacatgc
    11161 agcccgtggt gcgtttcaag accccgctac agggcgccgt gacctggaat gacctgatca
    11221 aggggccgat caccatccgc aacgaagaac tcgacgatct ggtgatcgcg cgcggcgacg
    11281 gcacaccgac ctataacttc tgcgtggtgg tggacgactg ggacatgcgc atcacccacg
    11341 tgatccgcgg cgacgaccat gtcaacaaca ccccgcgcca gattcacatc ctcgaagcgc
    11401 tgggtgcgca actgccgcag tatggccatg tgccgatgat cctcggcacc gacggcgaac
    11461 gcctgtccaa acgccatggc gcggtgagcg tgctgcagta tgaagaagac ggctacctgc
    11521 ccgaagcgct gctcaattat ctggcccggc tcggctgggc gcacggcgac gatgaactgt
    11581 ttgacatgaa gcagttcgtc gaatggttcg acctcggcgc catcagtcgc tcgccggccc
    11641 gtttcaacgg cgagaaactg gcctggatca accagcagta cctcaagtct gccgacccgg
    11701 tccggctggc acggctcatc cggccaaacc tcgaacacgc acttggacac gccgcctgca
    11761 cccccgcgct cgatgaagtc gctgcgctgc tgcgcgatcg ctgcacctca ctcatcgaac
    11821 tggccgatgc cgcacacctg ttctaccgtt tcgtgccacc aacaccggcc cagctcgctg
    11881 aaacactcga ccgggcagcc ctgccggcac tagccagcct gctgcaacgc ctgacggcgc
    11941 aggacggctg gcaacgcgaa gcgctgcacg acgccattcg ccaggcagtc gcagacagcg
    12001 gactcaagat gccgaaaatc gccatgccgc tgcgcctcat ggtcacaggc agtacgcaga
    12061 cgcctgccat tgacgccacg ctggcactgc tcggacgcga caccgtgctg caacggctgg
    12121 aacaggggtt gcgtcaagcc ggatgaatgt ccggctgggg tgtggcaccg caaggaccct
    12181 ttggggcgcg atgattaaat ccgcgtgatc gcgacgccgg actggcggga tggattgcag
    12241 ggcgcaattc gcagtcaatg gctgccctcc tgacaggaaa tgcaac
//
LOCUS       DAIANL010000087         7442 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1937664, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000087 DAIANL010000000
VERSION     DAIANL010000087.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937664"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1500 a         2457 c         2028 g         1457 t
ORIGIN      
        1 ctctggcaga attcacccat tttgcgcgag tttcatacct gatgcgcacc gatgaattcg
       61 attttgaact gccttcccgc ctgatcgccc gctttcccgg cgagcagcgt gcttccagcc
      121 gtctgctgca tgtagacgcg cctgcggcgc gcctgaccga cagcgtgttt tcggccctgc
      181 ccgagcagat gcgtgccggt gatgtcgtgg tattcaacga cacccgcgtc atcaaggcgc
      241 gactgttcgg ccgcaaggac agcggcggcc aggtcgaact gctggtcgag cgcatcacgg
      301 acgaatgtga agcgctgtgt ttcatacgcg caagccatgc cccgcgtgcc ggcgcgcaca
      361 tcatactggc cgacaactcg gtacttgagg tgctggcacg cgaacacgat ctgtatcgtc
      421 tgcgctttca cggcacaccc ggcgtactcg acacgctcga acgcatcggc gaactgccgc
      481 tgcccccgta tctcgaacgt ccggcgcaag cagaggatga tgcgcgctat caaaccgtat
      541 tcgctcgtca tccgggcgcg gtggccgcgc cgaccgccgg cctgcatttc gacgaggcga
      601 tgctgcagcg actcgatgac atgggcgtgc tgcgggtaca tgtgacgctg catgtcggcg
      661 caggcacctt ccagccggta cgcgccgtac acattgccga ccatgtcatg cacagcgagc
      721 gttaccatat ccccgacatc acggcccgga ccatcgccga tgcacaacgc gacagacggc
      781 gcatccttgc cgttggcacc accagcctgc gtgcgctcga agctgccgcg ccagatggca
      841 aggtgcacgc cggatatgga gaaacccgga ttttcatcac gcccggctac cgctttcgcg
      901 tcgtcgaccg tctgctgacc aatttccacc ttccgcgctc aacgctgctg atgctggtga
      961 gcgccttcgg cggcgttgaa cccatgcgtg cagcctatgc ccacgccatc gccaacgaat
     1021 accgtttttt cagctacggc gacgccatgc tgatcgagcg cgccctcacg agctgaccac
     1081 catgcatttt accttgcatc gcaccgatgg ccgcgcccga cgcggcaccc tgaccctgac
     1141 ccacggcacg gtggaaaccc cggccttcat gcctgtgggc acttatggca cggtcaaggc
     1201 gatgtcaccg cacgaactga ccgaactggg cgcccacatc gtgctcggca acacgtttca
     1261 tctctggctg cgccccgggc tggaagtcat cgccgcacac gacggtctgc accgcttcat
     1321 gggatgggac cgccccatcc tcaccgattc aggcggtttc caggtgttca gccttggggc
     1381 gctgcgcaag atcagcgagg aaggtgtgcg cttcgcctcg ccgctcaacg gcgatcgcct
     1441 gatgctcacc ccggaaacct cgatggcgat ccagcgtgtg ctcaattcgg acattgtcat
     1501 gatcttcgac gaatgcacac cctatccggc cgacttcacc actgccgccg agtcgatgcg
     1561 gctgtcgttg cgctgggccg aacgttcgaa acaggcccat gccggcaatc ccaatgcgct
     1621 gttcggcatc gtgcaaggcg gcatgcacga aacgctgcgc gacgaatcgg cgcgccagct
     1681 gattgacacc ggtttcgacg gctacgccat cggcggcctg agcgtaggcg aacccaagca
     1741 ggacatgcgg cgcatcctgc agcataccgc accccaattg cccgcagatc agccccgcta
     1801 cctgatgggc gtaggcacgc cggaagatat cgtgttctcg gtgcagcacg gcatcgacat
     1861 gttcgactgc gtgctgccca cacgcaacgc gcgccatggc atcctgttca cgcgactcgg
     1921 cgaactgcgc atcaaaaatg cccgcttccg taccgatacc gcccctgtgg atccgcaatg
     1981 cgaatgctac acctgccgtc atttcagccg tgcctacctg catcacctgc accgctgcgg
     2041 cgaaatgctc ggcgcacggt tggccacgct gcacaacctg cactattacc agcagctgat
     2101 ggcaggcttg cgcgcagcca tcgccaatgg acaactggac agctttgttg ctgcatttca
     2161 tgagcagcgc ggcggatcgt gctagaatcg cctcgttccg caccctgaca tttacggagt
     2221 ccgcacatgt tcattaccag cgcatgggcc aatacggccg cgtcgcaacc caccccgatc
     2281 gacactctgg tcagctttct gccgcttgta gtcattttca tcctgttctg gttcatgatc
     2341 atccgtccgc aaatgcagcg caccaaggag caggcaaaaa tggtagaggg gctgcaaaaa
     2401 ggcgacgaag tggtcaccag cggggggcag accggtcgcg tggtcaaggt gggcgaacag
     2461 tttgtctccc tcgaaatcgc acccggcgtg atcaccccca tccagaaaac cgccatccag
     2521 accatgctgc ccaagggcac aatcaaagac ctctgaggac tgacccccgc ttccgccatg
     2581 aaccgattcc cgctctggaa aaacctgctg atcgtcgcga tcctgctggt cgcattcatt
     2641 tatgcgctgc ccaacgtctt cggagaggca cccgcggtac aggtgacgcc cacccgtgct
     2701 gcgttccacg ccgatgcccg gctggtgcag gaaatcaagg atgaactcag cgctgcacac
     2761 attccctggg acgacgcgga agtcagcgac gacagcgtca aagtccgctt ccagagcact
     2821 gactggcagc tcaaggcaga agatgtgctg caaaaccgct tccgcgatgg ttacagcgtc
     2881 gcgctcaacc tgatatcggc ttcccccgca tggctcaccg ccattggcgc caaaccaatg
     2941 tatctcggcc tcgacctgcg cggcggtgtg catttcctgc tgcaggtcga catgcaggcc
     3001 gcgcttgaca aggcagtaca gcgcaatgcc aacgacctgc gcagccagtt gcgcgaccag
     3061 cagatccact acagcggcct cgacatcagc catcagaaca tcgtgctgca tttcgccgat
     3121 gcggcgacac gcaatcaggc cgccagcttc atcgcacaca actatcccga cctgatgttc
     3181 aatccacgcg acaacggcac cgatttcctg cttgaaggcg ggctacgcgc cgatgccatc
     3241 aagcgtctgc aggacagcgc cgtacagcaa aacatcacca ccctgcgtaa ccgcgtcaac
     3301 gaactgggcg tggccgaacc cgtgatccag cagcagggta ccgaccgcgt ggtggtcgaa
     3361 ctgcctggag tgcaggacac ggccaaggcc aaggaaatcc tcggccgcac cgcttcgctc
     3421 gaaatccgca tggtggatga aagccatgac attgccgccg ccatgcaagg ccaggtgccc
     3481 ttcgacgacg acctgtacac cgagcgcaac ggcatgccga tcctggtgaa gaaaaccgtg
     3541 ctgctgaccg gtgactacat caccgacgcc tcgcccggtt ttgaccaaca gtccggccag
     3601 gccgcggtgc acgtcaacct cgactcgcgc ggcgcgcgca ttttccgcga cgtcacgcgc
     3661 gagaatgtcg gcaagcgcat ggccatcctg ctggttgaaa aaggcaagac cgaagtcatc
     3721 accgccccgg tgatccgcga ggaaatcggt ggcggccgcg tacagatcac cggcatggaa
     3781 tccgcccaag aagcctccga cactgccctg ctgctgcgcg ccggtgccct ggctgcaccc
     3841 atggacatcg tcgaagagcg caccgttggc ccaagcctcg gcgctgacaa catccgcaag
     3901 ggcttccatt ccaacctgtg gggtttcgtc gccgtcgtcg gtttcatgat tctctattac
     3961 cggctgttcg gcatgttctc cgcgctggct ctcaccgcca acggcctgct cctggtatcg
     4021 ctgctgtcgg tcatgcaggc cacgctgacc ttgccgggta tggctggtat cgcgcttacg
     4081 ctgggcatgg ccatcgacgc caacgtactt atcaatgaac gcgtgcgtga ggagctgcgt
     4141 aacggcaact cgccgcaggc cgcaattcac gccggctacg accgcgcctg gggtaccatt
     4201 ctcgactcca acatcaccaa cctcatcgct ggtgtcgccc tgttctggct gggctcgggt
     4261 ccggtgcgag gcttcgcagt cgtgctgtgt ctgggcatcc tcacctctat gttcagcgcg
     4321 gtcacgctct ctcgcgctct ggtcaacctg acatacggtc gccgtcaccg gctgtcgtcg
     4381 atctccatct gagggcctgc gcatggaatt cttccgcatc cgccgtgaca tcccgttcat
     4441 gcgttacggg aagatcacta cagccatttc actgctgaca ttcctgttcg ccgtatgggc
     4501 attgaccttc aagggactca atctcggcat cgactttacc ggcggaacgc tgatcgaagt
     4561 caccaccagc cagcctgcca acatcgatca catccgcacc agccttgatc aggccggttt
     4621 tcgcgacgtc acggtgcaga acttcggcgc cagcaacgac gtgctgatcc gcttgccagt
     4681 gcacggcaat gccggcaacg gcaagaccag cgaagccgtc atgaatgtgc tgcatgtgca
     4741 ggaacccggc ataacgctca agcgtgtgga tttcgtcggc ccgcaagtgg gtcacgaact
     4801 gtatgacaac ggcgcgctgg cgctgatggt ggtggctgga ggcatcctcg tctacctcgc
     4861 actgcggttt gaatggcgct tcgccctggc ggcaatcatc gccaacgtgc atgatgtggt
     4921 catcattctc gggtttttcg cctttttcca gtgggaattc agcctgacgg tgcttgctgg
     4981 cgtattggca gtgctgggct actcggtcaa cgaatcggtc gtggtattcg accgaatccg
     5041 cgaaaacctg cgcaagatgc gtaagaccga tattccggaa atcatcgaca acgccatcac
     5101 cgccaccatg agccgcacca tcatcaccca cggcatgacc gaaaccatgg tgctggccat
     5161 gctgttcttc ggcggcgatg cactgcataa ttttgcgctt gcgctgacca tcggcatcct
     5221 gttcggcatc tactcctcgg tgctggtggc ctcaccgatg ctgctcatgc tgggcgttcg
     5281 acgtgagcac tttatcaagg ctgccacgtc gaaaacaggc gtgtcggcag acggcgcaca
     5341 agtctgagag cgcaacagcg ggagacatca tgagcaacag tgacccgatc gaactcgaca
     5401 aattcagcgc gctggccaac aaatggtggg acccgaacag cgaattcaag ccgctgcacg
     5461 acatcaaccc actgcgactg gattacattg accgtcacgc cggccttgcc ggcaagaccg
     5521 tgcttgatgt cggctgcggt ggcggtatcc tcgcagaatc gatgtcgctg cgtggcgcac
     5581 aggtcaccgg tatcgacctg gcagaaaaat cactcaagat cgcccgtctg cacctgctgg
     5641 aaagcggtgc gcaggtcgat taccgatgtg tggctgtcga acagcttgcc accgagcaac
     5701 ccggccatta cgacatcgtc acctgcatgg aaatgctcga acacgtgccc gcaccggcct
     5761 caaccattcg tgcctgtgcc gaactggtga aacctggcgg atgggtgttt ttttccacgc
     5821 tcaaccgcaa tccgaaatca tttctctggg ccatcgtcgg cgccgaatac ctgctcaaaa
     5881 tgttgccgcg cggcacgcac agctacgccc gcttcatcaa accctcggaa ctggcacgcg
     5941 atgcccgtct gagcggcctc gatgtggtcg acctgagcgg catgacctac aacctgcgca
     6001 acaagaccta cagtctggaa cccgacacca gcgtcaacta cctgatggcc tgccgtcgca
     6061 atgattgaag ccattctgtt cgacctggac ggtacgctcg tcgataccgc acccgacctt
     6121 gcctgggtgc ttaatcagca gcggcaaatg cacggcctca tcgaaatgcc ctttgacctg
     6181 atccgcccat gggcctcgca cggcaccgcc gggctgctcc aggcaggctt cgccatcgaa
     6241 ccagaccacc cggcctacga cgaattgcgc gaagaatatc tgcgcatgtt cagcgagcat
     6301 gataaccaat acagcacgct gttccccggc atgatggaat tgctggacga actcgaacga
     6361 cgcggcatcc cctggggcat cgtcaccaac aaacccggct ggctcactac cccattactc
     6421 gaccggctcg ggctcgacac tcgcgccgca tgcatcatca gcggcgatag ctgcgaacgc
     6481 agcaaacccc atccagaccc attgatgctc gcctgcgcac aactcgacct ccccgccgat
     6541 gcctgcgtct acatcggcga cgcggagcgc gacatccagg ccgcacacgc ctgcggcatg
     6601 cccgccattc tcgccgagta cggctatatc ggcccccacg acgaccctga aaactgggaa
     6661 gccgaaggcg tcattgccat ccccaccgac ctgttgctct acctcgaata aacccgctcg
     6721 gcgcactatt gtacgagccc gcatacaaat ttgctgtaga atgacatttg gctgaacttt
     6781 tgtcatatcg tccagtcata gcagcatcaa catgggggcg acctggcttc gacgtgggtt
     6841 gcgaaacaga tcagtgcata ccgaggacgg attacctcgt aaatccaacc gaatcgttta
     6901 actgcaaacg acgaaactta cgctctggcc gcttaatccg gctagaccct gcaccagtct
     6961 gcatgcggac tgggccgagc aatcggcagc agggtaatcg cagcatgctc gcgacgttca
     7021 gggttacttt gaacgttgtt aaagacaagg taactcgctg gtgtccagcc tgttcggggg
     7081 gcgtgtcacc ggctaaatcc aaatcaccgg actaagtatg tagaactggc tgcagagggc
     7141 ttgcggacgc gggttcaatt cccgccgcct ccaccattta accttgcaaa gatactctct
     7201 ttgcattccg acgaacctcg ctagacgcca cgtctcgcgg ggtttttcac ttctgcttgc
     7261 ggactttgtt ctcgactgca cgaccagttt ccctgctttt tcgctccgtt ctcactctct
     7321 attctctgtt ttctgcggac gcgcaaagcg ccaagtccac aagacagcca cttttcaatt
     7381 catggggttg cgcaagtttt gttacggtca tttttatcga gcattgcttc gccgctggca
     7441 aa
//
LOCUS       DAIANL010000088         5803 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1332583, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000088 DAIANL010000000
VERSION     DAIANL010000088.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332583"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1005 a         1725 c         1942 g         1131 t
ORIGIN      
        1 cccctgcgaa tcttgcaggg tataacccag ccggcaggct tgcgaacagt cgccgcgatt
       61 ggcgctgcgg ccggtctgtg catggctgat gtagcattgg ccggagtagg cgacgcacag
      121 cgcgccatgg atgaaatatt ccagtatcac gtcctgtggc actgcggcgc ggattgcggc
      181 aatctgatca agatccagtt cccgcgccag cacgagctgg gaaaagcccg catcggcaag
      241 aaaccgggct ttttctgcgc tgcggatgtc gcattgtgtc gaggcatgca gttcgatcgg
      301 aggcaggtcg agttccagca tgcccatgtc ctgaacgatc agcgcatcca cgccgacgtc
      361 ggccagttgg tacaccagag tgcgtgcagc ttcgagttcg gcgtcgtgca gaatggtgtt
      421 gagtgtggca tagatgcgca cgtgaaagcg gtgggcgaat tcgaccagtg cggcaatgtc
      481 tgcaacgctg ttggccgcgc cattgcgcgc accgaacgtg ggaccgccga tgtagacggc
      541 atccgcgcca tgcaggatcg cctgcttgcc gatttctgca gtacgggcgg gagcgagcag
      601 ttcgagcggt tgcggtggca tggtgcggtt ctggtcgtgt tgatcgggtg attctagcaa
      661 attcttcccg gtttctgccg cgtgccgagc cttacacggc agatgtcggg ttgcgatagc
      721 cgagtcggga cgacaattcc tgtccggcac gcatcagcaa cggaatccac tcgtcgcggc
      781 ggcgttcacg tggcgcggag atcgacagcc cggccaccac cgcgccggtg ctgtcacgta
      841 cgggtactgc gatacagccg atgccggttt cggcttcctc gttgtcgaag gcgtatccct
      901 ggtgcagggc ggtggcagcg ttgtcctgca actggcggac atcggtaatg gtgttcggcg
      961 tgaacgaggg taaaccggtg cgctgggcgt aatcggcgca ggactccctg ccgccggcac
     1021 ccagcatcag ctttcctacg gcggttgcgt ggagcggtgc gcggctgccg accacctgtt
     1081 ccacccgcat cagccgatgc gatactgcac gttcgatata gatcacctcg tcgccttcgc
     1141 gcacggtgag gttgacggtt tcgccggtgg catcacgcag tccggtcatc agcggcagtg
     1201 ccagcgggcg caggtctgcg tgctggctgg cctgcagtgc atagcgcatc agcccggcac
     1261 cgaggtggta accgccggac gggtcgcgcg ccacccagcc atgctgcgtc agtgatgcaa
     1321 gaatgcggtg cgctgtggaa ctgtgcaggc cggtgtcggc ggccagcagc ttgagtccgg
     1381 cttcgccgtg gcgtgccagg ctgtcgagca acgccaccat gcggtcgatg acctggatgg
     1441 cggaagggtc gggtcgggag gattcgctca tggcgatagt atatcttatt ccgcattatg
     1501 aaatcaattc tgtattgcgg aatccagact gttttggtaa ggtatcactt cctaccccaa
     1561 tttcaggagc agcacatgaa cgcccccgcg ttttgtcacg gcattcgcca ccacggcagc
     1621 gactggcccg agtttgaaga catggccgct tgcgcggtca tcgaaccgca ggccgtggcc
     1681 atttccgaca gcggcgaccc acgtgcggtg taccagacgc tgctgggcgc ggatgcgctg
     1741 cgctatctga cactgcaggt gacgggatcg aaggggtccg gtcacccggg cggatttgct
     1801 tccagcgctg aggtgcatgc cgcgctgatc atgctcgggc acaccaatat cgtgaccgaa
     1861 gtggggcacc atgcgccggg attctactcg tccatgttcc ttgatggatc gctggaggcc
     1921 atgggtatcc atactgtcga ggacatgatg gcgcgcttcc gtgagcgtca tggcctgctg
     1981 gggcacctgt cgggcgccat tcccggtctg ctcgccccgg ccggaccgct cggccagggg
     2041 cagcatttcg ccatggccgg cgcgttgctt caccctggca agctttttcc cgtaaccata
     2101 ggcgacggcg gcatgggaga gccctatgtg ctcaactcga tgatgcattt ccacaccgcc
     2161 tacccggatg cgacgaattt ccttcccgtg ctcgtctgga acggctacag tcaggaacac
     2221 cattcgatgg tctcgcgcat gagtaacgca cagatgaccg attattggcg tggtcacggg
     2281 ttcgaggata tcgtgctcgt cgatgcccgc gactacgatg acgccgggca ggaatcgccg
     2341 tatgtggata gcacgctgtt ttcatttgat cagcgccttg ccttcactca agccgtgctg
     2401 caaggtgtcg accgtgcctc ccgaagcgcg ctggccggtc ggctgacggt gttcatcatc
     2461 aagcagctca agggtgcggg tgtgcatgcc agcggcgcaa aatcgcacaa cctgtatccc
     2521 agggacacgc tcgaccagcc gcacattgcc gagagtctga agcgtcgggc attgccggtg
     2581 ggtgcatggc agatcgtgcg tgacaatttt tcccgcgccg gaggtggccc ggcagcccag
     2641 tgtgcggtga ccgagcgcga actggccaac gcgccaatgc cggaactccg gctgcacgct
     2701 tatgctcccg gggagtctgc agtaccatcc acggcgatgg gcgaactggt ggcccagctc
     2761 ggtcaggccg atccgcgatt catcgtcacc aatgccgacg gcaacgaggc ttcggcgatg
     2821 aagaacatca atgatgcgct gaaaatccgc catccgtccg ccgacccgct ctataaccag
     2881 gtaccggaag ggcaggtgta cgaaccgctc aatgaagacg cctgcgccgg cctcgctgcc
     2941 gggcttgcgc tgttcggggc gcgttcattg tggttgtcct acgaatcgtt tgccatcaac
     3001 ggctggccca tcatccagac ggtgacccag gccatggcag aactgcggcg ccggaccccg
     3061 tccgtggtgt gcatgtttac cgccggtgcg ctcgagcagg gccgcaatgg ctggacccat
     3121 cagcggcctg aaatcgaaaa cctgtttgcc ggcatgatgc gtaacggtaa cgtctatctg
     3181 ctgttcccgt gcgatgccaa ctcgatccag acggcctacc gccacgcagc caccagcgtc
     3241 aacaagggca tggtgatcgt cgcttcgaag agtgccctgc cagtgcacat gacgctggaa
     3301 cagtcgcaac aggccgttga ggaaggcgca gggctgatct accgcagcgc tgcagggagc
     3361 cgcggtcatg tcgtgtttgc tgcgaccggg gacatgatcc tgcagccggt gtttgcggcc
     3421 cgcgacagac tggaagcaga ggggtgtcgc gtgagcatcg tttcggtggt caatccacgc
     3481 ctgctgtacc gtccgaccga tgtggcctgg gatacggtat ccgagagcga ggcaggattc
     3541 atggcggatg aacgtttcaa cgaactgttt gatgctgacg tgctgatcgg agctaccggt
     3601 ggtccgagcg cgccgctcga accggtgctg ttgcgcaccc gcgcgggccg tcgagacgta
     3661 ttctgctgga agcgtggcga aaccacggcc tcagccgacg aactgatgca gttcaacggg
     3721 ctgacggcgc aggccatggc cggacgggca ctgcagttgc tggacggagc gcagcgttga
     3781 cagcgcccgt gcgcaacaag atcgtcgtgc tcgacgacga tccgaccgga tgccagaccg
     3841 tgcacagctg tctgctgctg acccggtggg atgtcgacac actggtcgaa gcattgatgg
     3901 acgcatcgcc actgatgttc gtgctgacca atacgcgtgc gctgaccgcg gaacgagcgg
     3961 cggcagtcac gcgtgaggtc agtacacatc tggtggccgc gctggagcgt ctggcgcaac
     4021 agggatgcgc ggtgcggccg ctgttcgtca gccgctccga ctccaccctg cgtggacact
     4081 atccggtgga aaccgacatc atgaacgaag tgctcgggcc gttcgatgcg catttcctcg
     4141 tgcctgcctt cttcgaaggc ggtcgtatca cccgcaatgg tacgcattac ctgatggtcg
     4201 atggcaagcc ggtaccggtg catgaaaccg aattcgcccg ggattccgtg ttcggctatc
     4261 accacagcca tctgccggat tatgtggcag agaagaccgc cggacgcatt ccagccgcct
     4321 cggtgcgcgt gctgacaccg ctggcgttga ccggcgagcc cggccggccg ctgcgcgtgt
     4381 tgcacgacaa tgcctgcgtg gtggtggacg cggagactcc ggctgatctg gagcgctttg
     4441 cccggcatgt ccatgcaagc gcaaccagtg gaaaacattg gctgttccgc agcgcggcga
     4501 gcttgctgac tgcgctggcc cggctggagc cgcagcccgt ggccgcagaa cacatggcga
     4561 cgtatacccg taatgggcgg cccggcgtgg tagtggtggg gtcgcacgtg ccgaccacga
     4621 cgcgccagct ggaacggctg ctgctcgaac cgggcgtggt gggcatcgaa atcgatgtgt
     4681 cgcgcttgcc gcacgatcgg caggcgctgt tcgatactgc ggtcgcggcg gtgtttcagg
     4741 cgtatgcccg cgacatgacg gctgtggtgt acaccagtcg caccgaacgg tcgtttgcca
     4801 gccagagcca gcggttggcg tttggcgaac aggtgtcggg gctgctgatg gatattgtac
     4861 gtgtcttgcc gcatgacatc ggattcctca tcagcaaagg gggcatcacc tcgaatgacg
     4921 tgctgagcga cggactggcc ctggccagtt cgcgcgtgct ggggcagatt ctgcccgggt
     4981 gtccggtggt gcgctgtccg ggcacccatg cccgctatcc tgatctgccg gtggtgattt
     5041 ttcctggcaa cgtgggcaat gaaacggcgc tggcgaccgt ctggcggcga ctgtccgggg
     5101 caatggccgc ataatggacc gtcagcgcga tgagctgatc gcccagctgc gtcaggtgct
     5161 gccgccggat gggctgtgcg ttgaagcatc catgctgcat gcatgggaat gtgacgggat
     5221 gtcggcgctg cgtcaggtgc cgtggctggt cgtgctgccg cagaccacgg cgcacgtgca
     5281 ggcagtgttg cgcctgtgtc gggagcacgg gattccggtg gtggcccgcg gtgccggcac
     5341 cgggctgtcg ggcggtgcga tgccgcatga gcgcggcgtg ctgctgagtc tggcgaagat
     5401 gaacgccatt cttgacatcg acccgttggc ctgcacggca cgggtgcagc cgggcgtgcg
     5461 caatctggcg gtttcggaag cggcacggcc acatggcctg tactatgcgc cggacccatc
     5521 atcgcagatc gcctgcacca ttggcggcaa cgtggcagag aactccggcg gcgtacattg
     5581 cctgaaatac gggctgacgg tgcacaacgt gatgcgtgtg caactggtta cgatggatgg
     5641 ctactgcctg gagctcggga cggaaacgct cgacagtccc gggtatgatc tgttggcact
     5701 ggccgtgggt tccgaagggc tgctgggtgt ggtcaccgaa gtcaccttgc ggttgctgcc
     5761 gcggccggag tgcagccagt tgctgctcgc cgcgttcgat gat
//
LOCUS       DAIANL010000089         6866 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2755447, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000089 DAIANL010000000
VERSION     DAIANL010000089.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6866)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2755447"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1270 a         1827 c         2280 g         1489 t
ORIGIN      
        1 cacggcggcg gccaagctct tccatgacgc tgccctggtt ggtctcttcc acgtccacgg
       61 taagggcctc gtagggttca tgccgttcgc cgttgacctc cttgaacacc acgcgtggtt
      121 tggagacggc aatttcaaat ccttcgcggc gcatgttttc aagcagaatg gtcaggtgca
      181 gttcgccgcg gcctgacacc aggaaagtat cggcatcgct ggtgtcttcg acccgcagcg
      241 cgacgttggt gagcagttcc ttgttgagtc ggtcgcgcac ctggcggctg gtgacgaact
      301 tgccttcggt accggccagt ggcgaatcat tgacctggaa gttcatggta agcgtgggtt
      361 cgtccacctt gagcagcggc agtgcaatgg ggttgtccct gtcgcagatc gtgacgccga
      421 tgccgatgtc ctcgatgccg ttgatcagca cgatgtcgcc agcctgtgct tcgtcgatca
      481 atacccggtt cagtccgctg aagccaagca cctggttgat gcgtccggat ttcggcgtgc
      541 tgtcagggcc ggccatgacg ctgacggtct ggcctggctt gatgcggccg ttgagaacgc
      601 ggccgacacc gatgcggccg acgaagctgg agtaatccag tgccgagatc tgcagttgca
      661 gtggcgcatc gggatcgcct tgcggtgccg gcacatgctt gagaatggtt tcgaacagcg
      721 ggttcatgtt ttcgcccggt tcatcgagtt cgagcttggc gtagccgttg agcgcggaag
      781 cgtataccac cggaaaatcg agctggtcgt cgttggctcc cagcttgtcg aacaggtcga
      841 aggtggcgtt gatgacgaaa tccggccgtg cacccgggcg gtcgatcttg ttgaccacca
      901 cgatcggctt gagtccgagc gccagggcct tgcgcgtgac gaagcgcgtt tgcggcatcg
      961 ggccttccac agcgtcgacc agcagcagta cgccgtccac catcgacagc acgcgctcta
     1021 cttcgccgcc aaaatccgcg tgccccgggg tgtccacgac attgatatgg tggtcgcggt
     1081 attgcaaggc agtgttcttg gcagtgatgg tgatgccgcg ttccttttcc aggtcgttgc
     1141 tgtccatgac acgttcgccc acgctctggt gcgaggcaaa agtgccggac tgctgcagca
     1201 gtttgtcgac gagggtggtc ttgccgtggt cgacgtgggc gatgatggcg atgttgcgga
     1261 gcaaacgggg catggtggac ggttccgtga atgggaaatt acgctggagc gcgtcccggc
     1321 ggttcgggcc gggacgggcg ctggcgtcgc tacagggtac cggtgccttg cggcattccg
     1381 gaaaacccgc aattgtagca tgttgtgcat gcaggaaatg tatcggcgca atccaaattg
     1441 cgttgccggg caaaaaataa tgggtcgcag cgataagcgg cttgtaataa aactgtggtc
     1501 aaggcttgcg aacgcgagga taacgtgtat aatgacccac tttccgcaag tgattctgtt
     1561 tggcaagaga cgcgggtacc cgttcgggtg cgacgcaatt cgccgggccg gcctgttttc
     1621 aggttcatca ttatatcatg gtgaactgtt gtggcccggg agaacggatc acctctgcct
     1681 ggcttgtggc gtgcgatgtg atgcgtgccg gccagtgtac catcgatccc tgacctttcg
     1741 cgttttggcg gtttcttcaa gcgcagtcat tccggttggc gacgatgttt ttcgcgccga
     1801 tatcgggtga tgatgcagcc tgtcatagtg tcggctttgc tttgcgtatc tttttagaat
     1861 gaggaaactg ctgtgtcttt tgaatcccta ggtttgaacc cttccatcgt caaggccgtg
     1921 accgacgccg gctacagcga agccacgccc atccaggcgc aggcgatccc gctgatcctg
     1981 caagggcacg atctgcttgc gtctgcgcag accggaaccg gcaagacggc tgctttcatg
     2041 ctgccggcgc tggaaaaatt gtctgccccg tcggcgacga atgtgcgtgg gccgcgcgtg
     2101 ctggtattga cgccgacccg cgaactggcg ctgcaggtga ctgctgcggc agaaaaatac
     2161 agccggcatc tgcggcgggt caaggtggtc agtgtgctgg gcggcatgcc gtacccggta
     2221 cagaaccgca tgctgtccca gccgtatgaa gtgctggtgg ctactcctgg ccgtctgatc
     2281 gaccacatcg agcgtggacg catcgatttt tcgcgccttg agttgctggt gctcgatgaa
     2341 gccgaccgga tgctcgacat gggtttcttc gaggatgtcg agcgcatcgc ttccgccacg
     2401 ccggccagcc gtcagacggt catgttctct gccacgttcg aaggcaatat cgcgcgtctg
     2461 gcgactgagc tgctcaagga cccccagcgt atcgaaatcg cgcatcagca ggcaaagcat
     2521 gaaaatatcg cccagcacat gcactacgtg gatgatctgg cgcacaagaa ccgcgtgctg
     2581 acgcatctga tcaatgatgc cgaaatcaac caggccatca ttttcaccgc aaccaagcgg
     2641 gatgcggata cgctggctga tgatctgcag gctgcgggcc acaaggttgc cgcgctgcat
     2701 ggcgacatgc atcagggggc gcgcaaccgt accatcgtac agatgaggca tggcaatatc
     2761 cgtctgctgg tggccaccga tgttgcggcg cgtggcctcg atgtgccggg tatctcgcat
     2821 gtcatcaact acgatttgcc gaagaatcct gaagattacg tccaccgtat cggtcgtacc
     2881 ggacgtgctg gcgccaacgg cattgccgtg tcgctcgcca gcccgcgcga tgcattgcag
     2941 ctcaagcgca tcgagcgttt taccgggcag gtgattccgg ttcaggtgat cgaggggctg
     3001 gagccacgct tcaagccgcg tgcacagacg ccgggtggcg gcaaacctgc tgcgggacgc
     3061 ggtggaaacc gttcgggtgg tcaggggggt ggcgaaggtc gtcgcttcca gaatcaaggc
     3121 gaatttcgcc gcggtcatgg cgaaggtcgt gcggcgcaga gcggattttc ccaggagtct
     3181 ggccggttcg gtcggcagca ggaagccggt cggggcggat tcggtcagga acgccgtcat
     3241 gggcacaacg atttttcctc gccaggcggg gatgcccgtc gcagccatgg tcatgcgggt
     3301 gaaaccggtg gccagcgcgg ccgtgatggc ggttatgccc gcgaccaacg cagcggcggc
     3361 acgggtaacc gggccgtaga tggtcagcat gatcaggcat gggcgcagac ttcgaccact
     3421 gcagcgattg cgcagcaacg tcgcaatacc ggtgatcgtc cggctcgtgg cggagcggct
     3481 ttccgtcgcg atgggcaggg ggcggcgcca tcgcgcccgg caggggtgcg aggcaatggt
     3541 aaccgttcct ggagctgatg ttcgggtggg tatcgattgg caataaaaac ggggcgcaag
     3601 ccccgttttt attgcggcat gttcgatttg atatcgaata atccgatggt aaccattcag
     3661 taaaatcgct tgcacacgtg ccgttgcatt ggctagccta gtgacgcggt gattaattct
     3721 ccacggatcg cgttgtgggt gaaatcgtga tgatcgcgaa ccggatgacg caggtcgtgg
     3781 tcggaaccac gatgccaggg aatccggcaa ggcgagggcg cgatttcacc ccaacccaca
     3841 ggggatgtgg ttctggcggg cagcctgctg cggtgcattt cttgtcaagg gcatcagcca
     3901 ttgatttcga atcgcgcctt gcagtccatc ctgccagacc agcgtcgcga ccgtgtggaa
     3961 ttcatcaccg catccctgga gctgtcgtgt gatggaggat gcaacatgag cgaagtgatt
     4021 gcggtgcggc gcgatgtcga aggatatctc gtcgatccgg aggactggaa tgagacgctg
     4081 gccgagcaac ttgccggtga agaggggttg tcgcttggtg cggcgtactg gccgattatt
     4141 catttcatgc gtgagtactg gcaggagcac caggtggcgc cggacgtgcg ccatgtggtg
     4201 gcgttcatgg ctgacagtcg ggacatggac aagaaaatgg ccaaggacga actgttcggt
     4261 ctgtttccgt atggttacgt gaagcaggcc tgcaaagtcg cgggcatgat gcgtccgcgt
     4321 ggctggagca cgggctgact ttccgttgcg acatgccgcc ggtggcgaga aggcggctca
     4381 ggccggcgga ttgtcttgcg tcaggcgttc gtagacaaca aaatcgtagc ggcagccgag
     4441 gctggcatcg tggtggatgt cgcgtgcggt ttcccgccat ttggctggat cgaatgacgg
     4501 aaaccaggca tcggcatccg cgatgtcgag gtcgatttcc gtcaggtaca tccgttgcgc
     4561 cacggacagt gcctgtgcat agatctgtgc cccaccgatg atgaatactt ctgcgtcccc
     4621 agcgcacagc gcgaggccct gctccagact cgaaaccgtg tgacatccgg gaaatgcctg
     4681 cgttgcgttg cgcgtgatca cgatgttgtt gcgctgcggc agtgcgcggc cgagcgattc
     4741 gtgtgttttg cgtcccatga ttacgctatg attcagggtg agacgcttga agtggcgcaa
     4801 atcttcagga agttgccagg gcagggtgtt gtcccgcccg atggcgcggt tgcgcgcggt
     4861 cgcggccacg agggaaacgg ttgggggcat gcggactcct ttttgcgtca cattataagc
     4921 gtaaaagcaa accggaatca gcgttgcgcg cagttttgcg ccggaatcga cctttatgac
     4981 ttcgcgacac ctcgacttgc ggcttggcgc catgcccatg cgggccttgc tggttgcgca
     5041 gttcctgtct gccctggccg acaacgccct gttggtggcg gccatcgcgc tgatgaaggc
     5101 attggggcag gcagaccatg tcagctgggt acagacgggt tttctggtgc cgttcgtgct
     5161 gcttgcgcca tacgtcggtg ccgtggccga tgccttgccc aaaggtcgtg tcctgctgat
     5221 tggcaacacc atcaagctgc tgggtaccgc gtcgatgctg ctgggtttgc acccggtatg
     5281 gtcctatctg ctggttggcg tgggtgcgac gctgtattca ccggcaaaat acggcattct
     5341 ggtgcagttt ttccgtcccg accgtctggt gcaggccaat gcactgctcg aaggttcgac
     5401 catcctggcc attctgctcg gcgtggtgct gggcgggtgg ctgactgacc gttcggtcaa
     5461 tcttgccatg tgggtcattg ttgccatcta tgccgttgcg gcattgacca acctgttcat
     5521 tccccgattg catccggtca gagtggagcg cacacactgg cacggctggc acctgctgcg
     5581 ctcatttggc cggtcgttgg tggtgctgtg gcgtgatgat gccgcgcgtg cgagcctgat
     5641 gggtaccagc ctgttctggg gcagtggcgc caccttgcgc ctgatgctgt ttgcgtgggt
     5701 gccgttggcg ctgcatatcc gcgacaatca gacgccggcg aacatgatgg gaatattgtc
     5761 ggtcggaacc atcgtcggcg cgatgcttgc ctggcgcttc atccatatgg acaggattcg
     5821 tcgcgcattc gtgagtggtc tgatgatcgg gccgtgcatt gcgctgctgg cgtggcagac
     5881 ccggctgccc gaagcctatg tctggtcgtt cgcactgggc gtgagcggtg gcgtgtatgt
     5941 tgtgccgctg aacgcgctgt tgcaggagcg ggggcgggtg tcggtcggcg ccgggcaggc
     6001 gctggcggtg cagaacctgt tcgagaacag catgatgatc gtcatggtca cggcttatgg
     6061 cttcatgacc aggtggccgg tgattgatgt gatcatggga tatggcgtgg cgatcctggc
     6121 gggcactggt gtgctggctg tactggcagg ccgtgttctg cgtcgtaatg ctgacacggt
     6181 cggggtcgaa tcgggcgata caggaaataa ttgaagcgag agattgacac aggatcgtaa
     6241 cgccgtataa tgcacggctc cacctgacga tgagtcgggt agtgagaagc tctttaacaa
     6301 aacgacgacc aataagagtg ggcatttgct gcggggaagt tggctgggac tggagttctg
     6361 gctgacggag cttagataaa aagcagcaag acccgcactt tgagagtgcg agcgagcctg
     6421 accgagaggt tgggtgagca gaaacaggaa agaactgaag agtttgatcc tggctcagat
     6481 tgaacgctgg cggaatgcct tacacatgca agtcgaacgg cagcacggac ttcggtctgg
     6541 tggcgagtgg cgaacgggtg agtaaagcgc cggaacgtat ccaatattgg gggataacgc
     6601 agcgaaagct gtgctaatac cgcatacgcc ctgaggggga aagctgggga tcttcggacc
     6661 tggcgatagt ggagcggccg acgtctgatt agctagtagg tgaggtaaag gctcacctag
     6721 gcgacgatca gtagctggtc tgagaggatg atcagccaca ctgggactga gacacggccc
     6781 agactcctac gggaggcagc agtggggaat tttggacaat gggcgcaagc ctgatccagc
     6841 cattccgcgt gagtgaagaa ggcctt
//
LOCUS       DAIANL010000090        10888 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_112316, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000090 DAIANL010000000
VERSION     DAIANL010000090.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10888)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10888
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112316"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1948 a         3082 c         3614 g         2244 t
ORIGIN      
        1 gcagggtccg gcccggtgcg cggggcgccg tagccggatc cgcggtatac cgttgcggtt
       61 ccggcccgga tcgggcagcg aggtgaatgg ccgtggaacg tttcgctccc gtgcgggctg
      121 atgacgccgg aattcggggt gccctgtgcg gtaggatgga cagaccaata tcatgcgggt
      181 cagatgtcgg cgcacagggt cggggcatga cagcgcgtga caacgtcggc caaagcgcag
      241 aaaatatggc gtggaacatg cgcggagaag atggatgcga acaaggaact ggctggcggc
      301 gttggcggtc ggcgtggcaa ttgcggcagt gctggtcggg ctgatgcaga aaccggcggc
      361 gccggaggtg caggcaaccg accttgccgg gcggccgttt tcactggcgg cactgcgtgg
      421 tcacccggtg ctgatcgatt tctgggcgac cacctgtccg gggtgtgtgc aggaaatgcc
      481 gcatctggtc gagttttatc gccagtacca cccggatggt ctcgaactgg ttgcggtggc
      541 gatgaattat gacgatgcag cgcaggtgcg caaattcgct gccgagcaag ggttgccgtt
      601 taccgtggtg atcgacagca cgggcgatat cgcgcatgcg tttggcgata ttcatctgac
      661 gccgacggcg tttctgatcg atgcccaggg cagaattgcc cagcagatca ttggcgaccc
      721 cgattttgcc gacctgggcc gccgcgtcaa ggctggctga gcgttcagcc ccgtcacagc
      781 agcggagtca ccaactgctt cagtcggccg tagtacacct gtccgtcgat gcgttcgcgt
      841 agtacgccct gacggtcgat gatgaacgag gtcgggatct ggcgaatgcc gccaaacacc
      901 tgttcgatgc cggcatcgga ccggccggcg atgaacggct tgccatgctc ggcgaggtag
      961 cgcgccgctt tgtccgggtc gtcgtcgagc gtcagtgtaa gcacggtgaa gccgcgaccg
     1021 tgccaatcac ggtagaatga ccggatttcc ggcatttcct tgaggcagta cggacaccag
     1081 ctggcccaga aattgaccag taccacctgg ccgtgcaggc tggatagcgg cagggtgtgg
     1141 ccatcgagca gttgcaccga cagtggcggc agtgagcggt gcatcggact gacgtgcgcc
     1201 ggcgggcgaa accagagcca caggacgagc gcgagcagga tgagcgtgac ggggctggga
     1261 atccagcgtc tgagccgggg ggaactgaaa tcgaattttc cggaatctgt tctggacatg
     1321 gtggagagag taaaggtgtt gtggatcaag gcgttgcata tatttttcgt ggtcagctgg
     1381 tttgccgggc tgttttacct gccccggctg ttcgtcaacc acgccatggt gactgatgcc
     1441 gccacgcgtg aacggttgtc gctcatggaa ggcaaattgt atcgcttcat gaccccgatc
     1501 ggggggctgg ctttgggatt tggcatctgg ttatggcgcg gttatggatt ctccgggttg
     1561 tggctgcaca tcaagctggc gctggtgctc gggctcgtgg tctaccatct gtattgcggc
     1621 aggctggtgc gtgtgtttgc cgagaaccgc aatatgcgca gccacgtgtt ctaccgctgg
     1681 ttcaatgaac tgccggtgct gctgttgctg gccatcgtca ttctggtggt ggtgaagccg
     1741 ctatgacacg tgctgccgct gtgatcaccg aacgtttctt cgctacctgc ccgcgcgggc
     1801 tggaagcgct gctggccgat gaattgcgcg cacttggcgc gcacgatgtg gacattaccc
     1861 aggggggagt cggctttgcc ggcgagtttt ccctgtgtta ccgcgtgaac ctgcattccc
     1921 ggctcgccag ccgcgtcctg tggcgggtgg gtggtggccc atatgctgac gaagacgaca
     1981 tctatcaagg tacgctgtca ttcgactggc cggcattgtt tgacgtgcag cgcaccatca
     2041 tggtgcgcat cactgcgcac aaatcaccgc tcagaagcct ggaatttgcc acgttgcgca
     2101 tcaaggatgc ggtgtgcgat cgtttccgcg aagcttgcgg cgaaagacct tcggtggaca
     2161 cgcgcgagcc gcaggtacgt atctatgcct ggctgaccgc agatacctgt acgctgtacc
     2221 tggacacatc cggcgcttcg ctgttcaagc gcggcctgcg caagcaggcc ggccttgctc
     2281 cgctcaacga aaatctggca gcgggcatgc tgctgctggc cggctggcat ccgggcatcc
     2341 cgttgtttga tcccatgtgc ggcagcggca cgatcctgat cgaagcggcg ctcatggcgt
     2401 tgcgcatggc gccgggcttg cggcgcagtt tcggcttcga gcatctggcc cgttttgatg
     2461 ccgcgctctg gcaggaaatc cgacaggccg cgctcgacca gtgcttgccg gtcacccgcc
     2521 aaccgattta cggccgggat ctgttcggcc ggtcgctcga cgatgcgcgc gccaaccttg
     2581 acgcggccgg gttggcgcaa gtggttgagc tcaaacaggg caacgtgctt gaagtgcctg
     2641 ccccggaagc cgaaggaatt ctcgtcacca atccgcctta tggcgtgcgt atcggtgaac
     2701 aggccgagct ggaggcgttc tatcccaggc tgggtgactg gctgaagcgt cagtgcgccg
     2761 gctggacggc ttatattctg tcggccgaca tgaatctgcc caaactcatc cgcttgcagg
     2821 ccacccggcg cattccgctc ttcaatggcg gactcgagtg ccggctgttc gagtaccgca
     2881 tggtggcagg gtcggcgcgc aagaagcagg ccgatgacgc agggcaggct gcggagccgg
     2941 gcgcgcccgg ctagatgatg ttgaggtggc tggtgccggc catcacgtcg cggtcgcgcg
     3001 attccgcgcc ttcaagtttg atgcgcagac gcagatcgtt gaccgagtcg gcgttgcgca
     3061 gcgcatcttc gtaggtgatc gcacccgcct cgtacaggtc gaacaacgac tggtcgaagg
     3121 tctgcatgcc cagttcgcgc gaacggctca tgatttcctt gatttcgctg atctggccct
     3181 tgaagatcag gtcggcgatc agcggcgagt tgagcaggat ttccaccgcg gcgatccggc
     3241 cggtgctgcc cttgcgcggg atcaggcgct gggagatgaa ggcgcgcagg ttgagcgaca
     3301 ggtccatcag cagttgctga cgcgcttctt cggggaagaa gttgatgatg cggtcgatgg
     3361 cctggttggt cgagttggcg tgcaacgtgg tcaggcacag gtgaccggtt tcggcgaagt
     3421 tcatcgcgta ttccatggtt tcgcggtcgc gcacttcgcc gataagaatg acgtccggtg
     3481 cctggcgcag cgtgtttttc agcgcgctga accagctttc cgtgtccacg cccacttccc
     3541 gttgcgaaac gatggatttc ctgtgctcgt ggacgaattc caccgggtct tcgatggtga
     3601 tgatgtggtc ctgggcgttc tcgttgcggt agccgaccat ggcagccagc gaggtggatt
     3661 tgccagaacc ggtgccgccg acgaaaatca ccagtccgcg cttggccatg gccacgtcct
     3721 tgagcacacc gggcaggttg agctgttcga gcttggggat caccgtggtg atggtgcgca
     3781 tgacgatgcc aatgcgaccc tgctgtacga aggtgttgac gcggaaacgg ccgatttcca
     3841 ccggagcgat ggcaaagttg gcttcgtggt gttcctcgaa gtcgcgccac tgccgttcgt
     3901 tcatgatgct gcgtgccagt tcgcgcgtgt gattgggtgt gagtgtctgg ttggacaccg
     3961 gcgagatctt gccgtccacc ttgatggcgg gcgggaaccc ggcggtgatg aacaggtcgg
     4021 aagcgcgccg gctcagcatc agccgcagca ggtcatatac cgtgttgaga gcgtccatgg
     4081 cggttatccc agaatcgagt ccttgtttgc cgcgcgcagg cgcgcttctg ccgggctgac
     4141 caggttgcgt ttgaccagat cctgcaggca ctggtcgagc gtctgcatgc cgacattctg
     4201 tccggtctgg atggaagaat acatctgcgc caccttgttc tcgcgaatca ggttgcggat
     4261 ggctggcgtg ccgatcatga tttcatgcgc tgcgacccgg ccggtaccgt ccttggtctt
     4321 gagcagcgtc tgcgagatca cggcgcggat cgattccgaa agcatggacc gcaccatttc
     4381 cttttcggcg gcaggaaaca catcgacgat acggtcgatg gttttggctg ccgaactggt
     4441 gtgcagggtg ccgaacacca ggtggccggt ttccgccgct gtgagcgcca ggcggatggt
     4501 ctccaggtcg cgcatttcac cgatcaggat cacgtccggg tcttcgcgca gcgcggaacg
     4561 cagcgcgttg gaaaacgaat gggtatgcgg accgacttcg cgctggttga tgacgcattt
     4621 cttgctctga tgcacgaatt cgatcgggtc ttcaaccgtc aggatatgcc cgtattccga
     4681 ttcgttgaca aaatcgacca tcgccgccag cgtggtcgac ttgcccgaac cggtggggcc
     4741 ggtgaccagc acgatgccgc gcggctggtt ggcgatttcc ttgaagattt tcggggcatt
     4801 gagatcttcg agcgtgagca ccttgctcgg aatggtacgg aacacggcgc ctgcgccccg
     4861 gttctggttg aaggcattga cgcggaaacg cgccagtgtg ggcacttcga acgagaaatc
     4921 gacctcgagt tgctcctcgt agactttgcg ttgaccgtca ttcatgatgt cgtataccat
     4981 ctcatgcacg acctgatggc tcatgtctgg caggttgatg cggcggatat cgccattgac
     5041 gcggatcatc gggggaagtc cggccgacag gtgcaggtcg gatgccttgt tcttgacggc
     5101 aaaggcaagc aggtcggata tttccatctt cggttcccgt tggttgtgcc gcgtattatg
     5161 aacacagtga aaagcacatt gcaagcgatc catcagcgca tcgccgatgc tgcggcgcgt
     5221 tccggccgcc cggccggggc ggtttccctg ctggccgtca gcaagacggt gacggcagat
     5281 gcggtgcgag ccctggctgc agccgggcag cgggacttcg gcgagagcta tgtgcaggag
     5341 gcgctgcaga aaatggccgg cctggccgac ctgccactgg tatggcattt cattggcccg
     5401 gtgcagcgca acaagaccac agcgattgcg atgcatttcg actgggtgca cagcgtcgac
     5461 cgcctgctca tcgccgagcg tctgtctgcc gcccggccgg aacagcgggg cgtcctgaac
     5521 gtctgcctgc aggtgaatgt cagtggcgag gcgggcaagt cgggtgtggc gccagacgac
     5581 gcggctgcac tcgcggctca agtcgcgcgg ttgccgcggt tgcgtctgcg cgggttgatg
     5641 gccattccgc gtgcgacctc tgaccgggcg acgcagcatg cccagtttgc cgcgctacgc
     5701 cattgcatgg aagccatgaa tcgtaacggc gcaggactgg ataccctgtc gatgggcatg
     5761 tcaggcgatc tcgaggccgc ggtggaagag ggcgcgacca tcgtaagggt gggcagcgca
     5821 ttgtttggcg cgcgtccggg caaccggata ccgcaggaag aggaacctgt ttcatgagca
     5881 tgcgcattct gtttgtgggg ggcggcaaca tggccacggc gttgatggcc gggttgatcc
     5941 gcgagggagt cgacgccaac cggctgtcgg tcatcgagcg caacgaggcc tcgcgtgagc
     6001 gcctgctggc gacatggccg gtgcgtgtat tcgacgaaat ggtggccgcg gcgttcgaga
     6061 cggacgtggt cgtgctcgca gtcaagccgc agcagctcag ggaggtggcc gggcagatcg
     6121 caccgctgct ggacggccag ctggtgctgt cggtggcggc tggcgtgcgc tgcagtgcat
     6181 tgtccggctg gctgggaggt tacgacgcac tggtgcgcgc catgcccaat accccggcca
     6241 ccataggggc cggcattgcc gggctgtacg ccatgcctgc ggtgagcgcc gcccagcgcc
     6301 tgaaggccac ccagatcatg caggccgccg gtgcggcagt ctggctggaa gaagaatctg
     6361 cgctggatgc cgtgactgcc gtatcgggca gtggtcctgc ctacgtgttt tatttcatgg
     6421 aagcgatgga gcgtgccgcg gtcgaactcg ggctgagtgc ggacatcgcc cggcagctga
     6481 cgctggcgac tttttccggt gcatcgcggc tggcgatgca cagtcacgat gcgccggcgg
     6541 tgttgcgcga acgcgtgacc tctccgggcg ggacaacgga aagtgcgttg ctgagcatgc
     6601 agactgcgga tgtggctggc gccgtgatcc gggccattgc cgcagcgcat ctccgttcca
     6661 gggaactggg tgatgcgttg ggataggccg cgtctgtcga ggcctcacgg tgcggggggt
     6721 gctgtggcta gccggaaatg gctatggtga tgttgcccgg cagcggataa catgttgcgt
     6781 tttctgaaaa tggcggcgat ggacgggcgg ttgaccggac gctatcgata tttgcatccg
     6841 cttctcagga gctgcgcatg gcttggcatt gacaagcggg gtcgtttgcc agcatgctgt
     6901 aatgacggcg cgtatcctgg cgcgggctgg cgcacgtttt gctgccgtgg agtcggtggt
     6961 gttgcgcgaa atcggcggtg cgcgacggta acaggattcg acaggatgtt cgcaaagggg
     7021 aacgattgaa catgccgcat aaacttggcc agggcgtggc gctgtaccag cagcgcaggg
     7081 accagatcct gctagccctg gaagcctatc gcctgtggct ggagcgcaat ggcgacgtgg
     7141 actccgaacg caatctgcgt ttgtacgaca tggcggaaag cctgcgacgc gacaagctga
     7201 tgctggcgtt tgtcgccgaa tattcgcgcg gcaagtccgt actgatcaac gcactgttct
     7261 tttccgatta caagcggcgg ttgatgccgt cagacatcgg ccggaccacc atgtgtccga
     7321 ccgaactgtt ctacgatgcc ggcagtgagc cttacctgcg cctgctgccg atcgagagtc
     7381 gtctgcgcga cgattcgatc tccgcgctca agcgtatgcc ggtcgagtgg agcagtgtgc
     7441 gcctcgaccc ggaagacgtg gagcagatgg cgcgggcgct gcgggcgctg gccgacgtca
     7501 agacggtgtc ggtaaccgag gcccaggcca tggggctgtg cagcgccgag gaggcgttgc
     7561 gggccggtgg cgcaggcatc gagattcccg cctggcgcta tgccatgatc aattttcccc
     7621 attccctgct caagagcgga ctggcgattc tcgacacccc ggggctcaat gcactcggtg
     7681 tcgaacccga gctcacgctc aatatcattc ccagcgcgca tggcgtgttg ttcctgctgg
     7741 ccaccgatac cggtgtgacg cgttccgatc tggaaatctg ggaacgcagc gtgcgccggc
     7801 acgccaacca ttatgtcgcc attctcaaca agatcgacat gttgtgggac gacatcaaga
     7861 ccgacgagga aatcgaggtc agcatccgca ggcagaagga acagacggcg cagcagctgc
     7921 acctgatgcc cagtcaggtc atcgcgttgt cggcgcagaa agcgctgctc ggcaagattc
     7981 gcggcgatca ggcgctcatc gagcgttccg gcatcggcga gctggaaaac ctgctggtgg
     8041 aaaagatact gccctcacgg caggagattc tctatcgctc ggtgtgcgat gaggtcatgt
     8101 cgatggtcga gatctcccgc cagggcatcg ccgaccagct catgcatgcg cagcgagatc
     8161 tggaacagat cgaaaccttt tccggcaaga atcgggaaat cgtcaccgag ttgcgtgaca
     8221 agctgatcca ggaaaaaaag gcctacgacc tgaccgcaac caattttcgt gttacgcgca
     8281 acgtcatcca ggaacagggg cagatgctgc tcgatgcgct ggccgaagaa gtgctcgacg
     8341 ccatgctggt caagggccgt gccacgctcg acgagtgctg gacgactccg gggctggtgc
     8401 gcggcatgcg cgggttgttc ggtcagatca ccgatcagtt ccacgaggtt gaatcgcgcg
     8461 cagagaacat tacccgtctg ctcaaggcgg cttacctgcg ttttcatcag gaacacagtt
     8521 ttccgctgct cgaaccaccc gtgctgagcc tggaaatcgg ccggcagaaa ctggccatgc
     8581 tggcacagca ggttgaccag ttcgcgcgtg acccgatgaa cctgatgatg gaaaagcgct
     8641 tcatggtgaa gaagttctac ctgagcctgg tgtcggaggc gcgcggtctg ttcgtgctgg
     8701 ccaaggagga agtcaaaggc tggttgcgtc gcagcatgga tccggtggtg atgcgcatcc
     8761 aggaccacaa gcgccagctc gaggtgcgtg tggagaatat ccgccaggtg cacgacaatc
     8821 tcgacaacct gcaggtgcgt gccgctgcct tgcaaagcca gcgcgcctcg ctggaaacac
     8881 ggctgcgtga gatcaatgcc atcatttcag gcatgggctg cagcgagccg gaaggcttca
     8941 gccgtagctg acgttgcgac gccggcccca ttcatgtggc cggtctgaca gcggcagagc
     9001 agccgtggtg cggcaatcca gcccgccaga ccgacgtctc gaccgtgtgg aatggatcac
     9061 cgcgtcctag agcaggacga tgtcatactg ttcctgcgtg tacagcgatt ccacctgcag
     9121 cgacaccggc ttgccgatga aatcggacaa ttgcgcgagg ctctgcgatt cctcgtcgag
     9181 aaacaggtcg atcacgggtt gcgaggcaag gatgcggtac tcgcgggcgt tgtactgacg
     9241 tgcttcgcgc acgatctcgc gcaggatttc atagcatacg gtctgtgcgg tcttgacctc
     9301 gccgcgtccc tggcacacct tgcacggctc gcacaggacg tgggccaggc tctcgcgcgt
     9361 gcgcttgcgc gtcatttcaa tcaggccgag cggagagaag tcgtgaatcg acacccgggt
     9421 cgggtcgcgg tcgagcgcct tgcggaattc gagcagtact tcgtgctgat ggtcgcggtt
     9481 gtccatgtcg atgaaatcga cgatgatgat gccgcccaga ttgcgcaggc gtaactggcg
     9541 tgcgatggac tgcgccgctt ccagattggt cttgaacacc gtgtcatcga aattgcgcgc
     9601 accgacgaaa ccgcccgtgt tgacatctac cgtggtgagc gcttcggtct ggtcgatgat
     9661 caggtaaccg cctgacttga ggtcgacgcg gcgcgccagc gcccgctcga tctcggcttc
     9721 gacggcgtac aggtcaaaca gcggtcgttc ggccgcatag tgctgcagtt tgccgacgac
     9781 cccatgggta tattgttctg aaaattcacg cattctttgc caggtttcac gtgagtccac
     9841 cagaatgcga ctgatttcgt tgccggccag gtcgcgcagt acgcgcaacg acaggttgag
     9901 atcctggtag agcatggttt tcatcggggc gaccttggcg cggccctgaa tgtccgacca
     9961 cagccggcgc aggtattcca catcggcgcg cagttcgctt tcttcggcgg tttcggccat
    10021 ggtgcggatg atgaagccac cggattcgcc ttcggcaatg acatgctgca gcttctgccg
    10081 caactgttcg cgaccggctt cgtcgccgat tttttgcgag atgccgatgt gagtgtcctg
    10141 cggcaggaat accagaaaac gaccggcgaa ggtgatctgg gtcgacaggc gcgccccttt
    10201 ggtgccgatg gggtccttga tcacctgtac catcagcccc tggccttcgg acaggatctg
    10261 ttcgatcggc cgggccgcct cgccgttctg gcggcgttct tcccagatgt cggctacatg
    10321 caggaatgcg gcgcgttcga ggccgatgtc gacgaatgcc gactgcatgc ccggcaatac
    10381 gcgacatacc ttgccgaaat agatgttgcc caccaggccg cgctgcgagc tgcgcttgac
    10441 atgcagttcc tgtaccacgc caaggtgcat caccgccacg cgggtttcct gtggggtgac
    10501 gttgatgagg atttccgcgc tcatgccagg gttcgcattc acaggtcagg tcaaaccgtt
    10561 aacgatatac cgtatatgcc attgcgtcca gtcgggcgtc gcgcggtgcg catgtcagtc
    10621 atgtgtcaat aaagacacag agccgtgtga aatttttgtg acaaccccgg gattttgacc
    10681 ctcgaatcct atactgccat cggccattcg ggtacggctg gcgcaggcat gcttgccacg
    10741 aagctgtacc gtgaatgttt ttcttctgtt aacaagggga tgtaagtgag caagcctttc
    10801 cttcgcacca aaatcagtgt gctggtggcc ggtgttctgg ccgttgggct ggcgcctgcc
    10861 gccatggcgg attcgagtga ccagatcc
//
LOCUS       DAIANL010000091        29283 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_929161, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000091 DAIANL010000000
VERSION     DAIANL010000091.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 29283)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29283)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29283
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929161"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5690 a        10049 c         8297 g         5247 t
ORIGIN      
        1 ggatcgccgc caccggcacc agcaggacgt gctggtgtac gccggcctgc agatggatat
       61 tgacgaattc cgaaggaaac agccggttgt tgcgattggc aaacgtcgcc ttgaacttgg
      121 ccgtaccggt ggtcggatca atcacgttat ccacggcaag cagcctgccg ctcgccagcc
      181 gcacccggtc atcacggtca taggcatcca ccacgtaggt ctggccatgc tgccagccag
      241 gcaacaccag accaatgaga tcggacggca caggaaacac cgcgtcaatc ggctgtgttt
      301 gggtgatcac caccagcccg gtcgtggccg cggcatgcac catattcccc tcgtcgacca
      361 gccgcaggcc gacgcggccg gtcaccggcg cggttacacg gctgaaatcc agttgcagcc
      421 tggcattggc gagctgcgcc tgatcggact tgaccgtgcc ttcgtcctgg cgcaccaggg
      481 caagctgcgt gtcgacctgc tggctgccga tcgaattctg cgccagcagt ttctggtaac
      541 gcgccagatc gaggcgcgca tcgtcgagta gcgcctggtc gtgcagcagc tgtccgaccc
      601 actggtcgac caccacctgg aacggtcgcg gatcgagcac ttccagcacg tcattcgcct
      661 tgaccaactg gccttcgcgg aacagcacat gctgcagcag accatccacg cgcggcgtaa
      721 tcgtcgctgt ggtgcgtgcc accacggtgc ccagcgcctc gatctgcacc tgcatgtcac
      781 catagtgcgc tgccgccacc tgaaccggca tcagtgcccc gccccgctgt gcgacatgct
      841 ccaccgacgc tgcatgcgtc tgccaccaca caattgccag cacgagcgct gcgcccaatg
      901 ccgtccctgc taaccatctt ctgtgtcgca tatccgtcat cccgctcgcc agtacgcacc
      961 gtaccgccac tttacttcgc tgcttccata cgcagccttc tgctgcacct aacccgtcaa
     1021 gtgtaccctg cgacaagcag tccagtatta cagatccctg aaacacgccc aattgtcacg
     1081 cctggctaca cctcatccgg cttcggtcag gggctcgatg ccaggcaggc tcatgaacgc
     1141 ctgctcgcgc agtgtcacga tgaaatcgtt taaccaggac tgcgcagcat cgttggcgcg
     1201 cacggcagcg tacaggtcgc tccacagccc gcctgaccgg atcggccgcg ccaccacgta
     1261 accgcgttcg agatatggcg ccacggccca ggccggcaac gctgccaacc ccttgcggct
     1321 ggcaaccagc tgcacgatgg cgatcgtcag ttccgtctgg cgccgctcgg gatgcacgcc
     1381 cgccggcttg agcacacgcc gcaccaggtc aagcagccga tcgggcgccg ggtaagtgat
     1441 cagggtttcg gcagcaaaat cctcggcctg gagaaagcgt ttggccgcca gcggatgcgc
     1501 actggccatc agtgccagca tttcgtagcg gaacagcggt tcgaatgcca cgtcacgctg
     1561 acgtccggct tcggacacga tgaccaggtc ggcgcggtcg gccgccagca agccggtcgg
     1621 atcggcgtga aacccgccga ccagatccat ctcgacctcg ggccagcggg tgcgatagac
     1681 atccatggcc ggcatcagcc agtcgtagca ggtgtgacac tccacggcaa tgcgcagccg
     1741 ccgggccacc ggattgcccg ccagacgggc cagatcacgt tccgcggcct cgatgcgcgg
     1801 cgtgatgtcc cgcgccagcg ccaacaggcg ttctccgccc gccgtcaacc gcatcggtcg
     1861 cacccgatcg aacagcggca tgccgtaatg ggcttccagt gccaccagct gatgcgacag
     1921 tgccgactgg gtcagataga gccgtgctgc ggcacgcgta atgctgcccg tcgcatgaat
     1981 ggcctcgatg acccgcaggt gcttgagcgt cagcatacgg actcgctcat gacctggccc
     2041 gcagcggatt ttgatgaaat aaaatcatgg ttcgcatcaa aacaattcgt ttgcatcata
     2101 tatcaaattc ccgcatcatg cacgttcccg ccactgaccg ttattggagt gatcgcatga
     2161 ccaccatcca caccctgggg tttccccgca tcggcaaaca ccgcgagctg aaattcgcgc
     2221 tggagaaatt ctggcacggc gaaatcagcg cggatgcgct tgaacaaacc gcgcgcgatc
     2281 tgcgggcgca acactggcag atcgaacgcg ccgcaggact cgattatgtc acggtcggtg
     2341 atttcgcctt ttacgaccag atgctcaacc acatccagtt gctcggttgc gaaccagaac
     2401 gcttcggctt tgccgccgac acgcccgaac tcgaccgcca cttcaccctg gcgcgcggcg
     2461 tggcgagcga aaccgcagcc tgctgcgccg gtcatgcaca ccatggcagc gccgcgctgg
     2521 aaatgaccaa gtggttcgat accaactacc attatctggt tcccgaattc accgacgcca
     2581 cccgtttcag cctcgcatcg acccggctgt tcgaccagat cgccgaggca caggcgctcg
     2641 gccacccggt caaggtcagc ctgatcgggc cgctgacctt cctctacctg ggcaaggaaa
     2701 aaaccgccgg cctcgaccgg ctgaacctgc tcgacgcgct gctgcccgtc taccagacca
     2761 tcctgcagaa actgcagtcg ctcaacgtcg cctgggtgca gatcgatgaa ccgatcctgt
     2821 gcctcgacct cgacgcccgc tggaaggcgg cctttgcgcc tgcgtatggc aagctggccg
     2881 gaaccggcgc acgcatcctg ctcgccacct atttcggcgc cctgcttgat aaccaggcca
     2941 ccacactcgg cctgcccgtc gccggctggc acatcgacgg cgtacgcgct ccggaacaga
     3001 tcgagccggt catcgccgca ctgccgtccg accgcgtgct ctcgctcggc ctgatcgacg
     3061 gtcgcaatat ctggcgcacc gacctcgacc gcgtgctggg cgtggcgcaa caggcacgcg
     3121 cccgtctcgg cgaccggctg tggctcgccc cgtcgtgctc gctgcagcac gtgccggtcg
     3181 atctgtccac cgaaaccgcc atggacgccg aactgcgctc ctggctggct tacgctgtac
     3241 aaaagctcga cgaactgggt acgctgaaaa ccgcgctcga ccagggcaac gatgctgccg
     3301 ccgaagcgct gacccgttcg cgcaccgcaa tcgcctcgcg ccgcagcagc ccgcgcgtgc
     3361 acaatccggc cgtggcccag cgccttgcca gcctgcccgc caatgccgac cagcgccatt
     3421 cgccgttcaa ggtgcgtcag gaagtacagc gcaaacgctt ccatctgcca gcttacccga
     3481 ccaccaccat cggttcgttc ccgcagacca aagccatccg tgccgcccgt gccgccttca
     3541 agcgccacga aatgaacgaa gccgactatg tgcaggcgat gcagtcggaa attcgcctgg
     3601 ccgtggaaaa acagatcgaa ctgggactcg acgtgctggt gcacggcgaa gccgaacgta
     3661 acgacatggt ggaatacttc ggcgagcaac tggccggatt cgccttcagc cagaatggct
     3721 gggtgcaaag ctatggttcg cgctgcgtca aaccgccgat catttacggt gatgtctcgc
     3781 gtccgcaccc catgaccgtg caatggagca gttatgccca gagcctgacc agcctgccga
     3841 tgaagggcat gctgaccggt ccggtcacca tcctgcaatg gtcattcgtg cgtgacgacc
     3901 agccacgcag cgccaccacc ctgcagatcg ccctggccat ccgcgatgaa gtcgtcgacc
     3961 tggaacaggc cggcattggc atcatccaga tcgacgaacc ggcgatccgc gaaggcctgc
     4021 cgctgcgccg tggccagtgg gacgcctacc tcgactgggc cggccgcgct ttccgcatct
     4081 cggcatcggg cgtcaatgac gaaacccaga tccacaccca catgtgctat tcggagttca
     4141 acgacatcct gccggccatt gctgccatgg atgcggacgt catcaccatc gaaacctcgc
     4201 gctccgacat ggaactgctc accggcttcg gcaagttcaa ctacccgaac gagatcggcc
     4261 ccggcgtgta cgacatccac agcccgaacg tgccggaaac cgaagaaatg gtccgcctgc
     4321 tgaagaaggc cgccgaagtc atcccggccg acaacctgtg ggtcaatcct gactgcggtc
     4381 tcaagacgcg cggctggccg gaaaccgaag ccgcactggc caacatggtc gctgcagcga
     4441 aagcgatgcg cgcatcggcc tgacgctcgg gcgttcgccg caccagacaa acggggcgca
     4501 ccagcgcccc gttttctttg cagcaaccga ccaccgtcac ctgcggcggg tcgggtacgg
     4561 ccagggttac tgcccgacgc cgccgggcga tccacccatc atccgcgtcc ctgcaccacc
     4621 gacaccattg gtggacatac ctccctgctc ctgcgactgc atgcgcgaac gcatgccctg
     4681 catgcgctgc tgcatggccg atcgcatctg ttgacggcgc gcctgttgct gttccggagt
     4741 catgctctgg tagttttgca tgttggattg catgttctgc atcgacggcc cacccgccgg
     4801 caccgcggtg gcatcatcgg cgcgggcgat accggcagca gccagcgccg acacaaacag
     4861 gaggggaagt gtgttgacaa tacgcatgat tgggctcctt ggaattggaa tgccggacaa
     4921 ctggtccgac ccgcccattg tgcgcacgcg ccatgaacag aatatgtcag aacggacgca
     4981 atgcgttaca agttgtaacc cattgccagg tcacaggtcg cgcagcccca gcacgtcctg
     5041 catatcgcac aggccggctc cacgagcagc caggaaacgt gcggcgcgca gcgcgccctg
     5101 ggcgaaggtc atgcggctcg atgccttgtg actgatctcc acccgttcgc cgataccggc
     5161 gaacaacacg gtatggtcgc cgacgatatc gccgccacgc accgtggcaa agccgatcgt
     5221 cgccgcagca cgttcaccgg tgatgccttc gcggccgtag accgcgcagt cggcgagatt
     5281 gcgcccggtg gccccggcca gcacttcgcc catgcgcaac gccgtgccgg aaggtgcatc
     5341 aaccttgtga cggtgatgcg cttcgatgat ttcgatatcg taaccttcgt tgagcactct
     5401 ggccgccagt tcgagcagtt tgaaactcag attgacgccg acgctcatgt tgggcgcaaa
     5461 cacgatgccg acgcgccgcg cggcggcggc aatgcgcgct ttctgctctg catcgaagcc
     5521 ggtggtaccg atcaccaggt tcactccgag acgttcgcac tcggccagat gcagcatggt
     5581 cgcctcggga cgggtaaaat cgatgagcac atcggcgcca gccagcgcgg cggggatatc
     5641 ggcgccgatc atcacgccgc cggccgccga ggcaaactgg ccgacatcct tgccgcccgc
     5701 gcgcacgagt gccgcgtgca gcacgcagtc ttccgccatt tcaaccgcct cgatgagcgt
     5761 cttgcccatg cgcccggaac tgcccgccac caccacgcgc agcatgggtc aacccttcgc
     5821 cgcggctggc gcagccggaa cgacgcttgc agcggctggc gcctgctccg gcattccgct
     5881 gcggtcaatg cgggccagct tgccttccgg gtcaaaatac agcgtcaccc gacgcgactc
     5941 cgccagtttg ccgtcatgct gcaagctgta gatataatcc caacggtcgg cgtgaaatac
     6001 atcgctgatg agtggcgatc ccatgatgaa ctgcacctgc tgacgggtca tgcccagctt
     6061 gagcttgtcc agcgcaacgg gatcgatgta gttcccctga cggatatcca tcttgtaagg
     6121 cgacatgaaa ctcagctgag acagcagtcc catgccgggc agcgtggagc atcccgagag
     6181 agccacaacc agcaaaccgg aacagagaaa aaacgacagg cgcatgacga gtatttgcaa
     6241 agtcgggcaa agccactatg atagcctaaa tcgtccgggg ataacacgca tcgccatgag
     6301 tctgccctcc aacctgaaaa gcatcggcct caaggccacc ctgccgcgtc tgaaggtgct
     6361 ggaactgttc gagcacagcg agaagcagca cctgacggcc gaggacgtct atcgcatgct
     6421 gatgaccgag gaaatcgaca tcggactggc caccgtctac cgcgtcctga cccagttcga
     6481 gcaggcagga ctgctcatcc gccaccactt tgacagcgac aaggcggtct atgagctcaa
     6541 cgcaggacag caccacgacc atctggtgtg cgtgcaatgc ggtcatgtgg aagaattctt
     6601 cgacgaggaa atcgagcgtc ggcagaaagc cattgctgcc gaacgcggat tttccattca
     6661 cgatcattcg ctgcatctgt acgtggactg caatcggagc ggctgcccca accggggccg
     6721 cggcaaatga catccgcacc gatcgtgctg tacggcatcg ccaactgcga caccgtacgc
     6781 aaggcacgcg catggctcga acgccacggc cttgacttcg aattccgcga tttccgcaaa
     6841 accccggtca gcgcgacgca attgcagtcc tgggcgacac agctcggctg ggacaccctg
     6901 ctcaaccgcc gtggcaccac ctggaggcta ttgcctgcgg ccgagcggga cacaatcacc
     6961 gatgcatcgg ctgccatccg gctgatgcag gcaaaaccgg ccgtcattcg ccggccggta
     7021 ctggaacgcg gtgcactgct gctgaccgga ttcgacgaac ccgcatggaa ccggctgctg
     7081 gccggccagg aacccactca tgtctgacac tctggatctg gcgcgcgaac tgatcgcgcg
     7141 cccttcgcgc acgccggatg atgccggttg ccaggatctg ctgcgcaccc gactggcgcc
     7201 actcggcttc cgtttcgaca cgctggtgtc gaacggggtc accaacctgt gggcgcggcg
     7261 cggcgacacg gccccgctga catgcttcgc cggtcacacc gatgtggtgc cacccggccc
     7321 cctcgacgcc tggcattcgg atccattcgt gcctaccgaa cgcgacggca acctctacgg
     7381 ccgcggtgcg gccgacatga aaacctcgat cgcggccttc gtcaccgcca tcgaaggctt
     7441 cgtgcacgag catccggacc accgtggcag catcgccctg ctcatcacct ccgatgaaga
     7501 aggcgttgcc agcgacggta cggtcaaggt ggtcgactgg ctcgaatcac tcggcacacg
     7561 catcgaccac tgcatcgttg gcgaaccgac ctgtgtcagc cacctgggcg acaccatcaa
     7621 gaacggccgc cgtggctcgc tgtccggcca cctggtaata cgcggtgtac agggacacat
     7681 cgcctatccg cacctggtgc gcaatccgat ccacctggct gcaccggcca tcgcagacct
     7741 ggcgaccacc gagtgggacc gcggcaacga atatttccca cccaccagct ggcagatttc
     7801 caacatccat gcgggtaccg gagccaccaa tgtggttccg ggcacgctga acatcgattt
     7861 caacttccgt cattccaccg caagcagccg cgaatcgctg caacagcgtg tgcacgccat
     7921 actcgaccgg cacggactcg actacgcgct gacctgggaa aactccggcg atccgtatct
     7981 gaccgctgcc ggaagcctgg tgcaggcaat ttcgggcgcg attaccaccg tcaccggtct
     8041 gcagcccgaa ctgtcgacca gtggcggcac ctcggacggc cgcttcatcg cacgcatcgc
     8101 gcaacaggtg atcgaattcg gcccggtcaa cgccagcatc cacaagattg acgaacacgt
     8161 cgccctgagc gacatcgcac ccctgagccg gatctaccag ctcactctgg aacagctgct
     8221 tgcgccatga ccaatcttcc ctaccccgac accgacgcgc tcatcacact gcgcgactgg
     8281 ctccgtttca gcgtgagcga aatgaaccgc aacagcgtgc atttcggcca cggctgcgcc
     8341 aatgcctggg atgaagcggt gtggctcacc ctgggcgcgc tgcggctgcc ccgcgaccgg
     8401 ctggaaccgt tcctcgatgc ctgcctgaca gagaccgaac gcgacatgtt gcgcggactg
     8461 ctgcaccggc gtgtccatga acgcgttccg gtcgcctacc tgctgcacga agcctggctc
     8521 ggtgaattca gtttttacgt cgacgaacgc gtcatcattc cgcgctcgtt catagccggt
     8581 ctgctgcgcg accgactgac gccgtggatc gacgaccctg aaaacgtcac ttcggcgctc
     8641 gacctgtgca ccggttccgg ctgccttgcc gtcctgcttg ccgatgcttt ccccaatgcc
     8701 gacatcgacg ccgccgatct gtccgaagac gcccttgcag tcgccgggcg caacgtacgc
     8761 gaatacggcc tgcaggaaca gatcagcctg atccgctccg acctgttcga aacgctcgac
     8821 cggcaatacg acgtcatcgt caccaacccg ccctatgtcg atgccgccag catggacgac
     8881 ctgccccccg aataccgcgc cgaaccgcgc ctggcgctgg cgggcggtag cgacggcctc
     8941 gacctggtgc gcaggatcat ccacgacgcc ccacggcatc tctatccgga cggcctgctg
     9001 atcgccgaaa tcggccacaa ccgtgatgcg ctcgaggccg aattccccca gctggaattc
     9061 agctggatcg aaaccgacgg cagcgacgaa tacgtgttcg tgctgacaca ggcccaattg
     9121 cagcaactct gaccgacgac cgcaacgcgc ggccgcacga acgtccgttg ccgcaaccgc
     9181 ccctcagtac gtcagtggcc agcggccagc cagatggtag gcgggtccgc tggcgccagc
     9241 aacggagcgg gcaagcacga attcgctcac gcgccagcgc agcgcgccat ccgcctgcag
     9301 tgccgccggg caacgcgcgt ttctcagcac ggtcacatgc ggaaaccatg gccgtgcatc
     9361 aaacgtcagc cccgccgcaa ccagctgttc gcgcagcgcc cgcaccagtt cggtcagcgc
     9421 ggcaggcggg ctggccggcg tcagccaggc gatgcggttg tgcggccacc agccgccctg
     9481 tgtcaactcg aactcgaacg gcgcagcgcg tactccgtgc gccgcctcgc gcacctgtgt
     9541 caatgtcggc tgcgcaacct cacccatgaa cgccagtgtc atgtgcaatg tcgattccgc
     9601 catgcgccgc ccaccgcact gcacgctcca gcgcgccccc acgcggtcga tcgcctcccg
     9661 gcaggccgca tccggccaca gtgcaaaaaa cagccgttcg ttcatctgcc gcgttccact
     9721 gtcagccacc gcacaacaca cattttctgc ttgaattcat cgccattgcc ctgatgtatg
     9781 cagcaacgca tattctgtca ggaaacatcc cacatgaata ccatccggcc ggctgccgta
     9841 gccggcctgt tttaccccgg aaaccgtggt acgctggccc aggaagtcgg caccctgctg
     9901 gcggcagcac gcccggacac agcaggccca tgcccgaagg tggtcatggt gccacatgcc
     9961 ggctatgtct attccggcgc catcgccgcc gcagcctatg cccggctgga agccagctgt
    10021 cacgacatcc gccgcgtggt gctgatcggt ccggcgcacc gcgtcgccgt acgcggtctc
    10081 gcattaccgc aagcggacgc attcgccacg ccgctgggca acgttccgct cgataccata
    10141 ctcatggcgc atctggccag actgccccag gtcagcgtca gcgctgcagc ccatgcccgg
    10201 gaacattcgc tggaggtcca gttgccattt ctgcagacgg tactcggcga attcagcctg
    10261 gtgccgctgg tcgccggcaa cgcgaccccg gacgaggtgg cgcaggtgct tgaactgtgc
    10321 tggggcggcg cagaaacact gatcgtcgtc agttccgatc tgtcacatta tctgccttac
    10381 gccgccgcac aacgcactga tcgggacacg ctgcagcaca tcctgcgcgg cgatgcctgg
    10441 ctcgacggcg aacaggcttg cggcagcatg gcggtcaacg gactgatgct ctcggcccgc
    10501 agtcaccggc tgcacgcgga actgctcgat gcacgcaatt ccggcgatac ggccggcgac
    10561 cacgagcatg tcgtcggcta tgcggccatc gcctatctgg agcctgacca tcatgactga
    10621 acgcgaaggc gaacaactac tgccgattgc gcgcagcacc atcgccgcca gcttcggcat
    10681 cgaactggca acgaatgagc gcgccgcctg gctgcacgaa ccgggcgcga gcttcgtcac
    10741 gctgacccgc gacggccagc tgcgcggctg tatcggcagc atcgaagcgc gccagccttt
    10801 gctcgcggac gtcaagcaca acgcgctcgc ggcggcattc cacgacccgc ggttctcccc
    10861 gctgacgcat cgcgagttcg accgcatcag gatcgaggtg tcactgttgt cgccgctcga
    10921 acaactctca ttccacaacg aagctcacct gctgtcacag ttgcgccctg gcaaggacgg
    10981 gctggtgctc gaattcggcg cgcaccggtc cacctttctg ccacaagtgt gggaacagtt
    11041 gcccgaacca cccgatttca tcgcgcagct gaaacgcaag gcgggcctgc ccggacagtt
    11101 ctggcacccc ggtctgctgg tgttccgcta tcacgttgat aaatggcaag aaaccattgc
    11161 ggagaacgca tgaatccatc ccaccatcct gcccgctact ggcacgccct cgacgacggc
    11221 cgcatccagt gcgacctgtg tccgcgcgac tgcatgctgc atgaaggtca gcgtggcgca
    11281 tgcttcgtac gtatgcgtga acatgaccgc atggtgttga ccacatacgg acgctcctcg
    11341 ggattttgcg tcgatccgat cgaaaaaaaa ccgctgttcc atttccatcc cggcagcagc
    11401 gtgctctcct tcggcaccgc aggctgcaac ctgtcgtgca aattctgcca gaactgggac
    11461 atcagcaagt cacgcgacat ggacacgctg atggatcagg ctgcgcccga agccatcgcg
    11521 ctggccgcgc aacagcatga atgcgcttcg gtggcgttca cctacaatga tccggtcatc
    11581 tttgccgaat atgccatcga cacggcactg gcctgccacg accacggcat acgcacagtc
    11641 gccgtcaccg caggctacat gcacgacgct gcgcgacggg atttctttgc ggtgatggat
    11701 gccgccaacg tcgatctgaa agcattttcc gatgagttct accgcaaggt gaccggctcc
    11761 cgtctgcaac cagtactcga cacgctgcgc tacatccgcc acgaaactga tacctggctg
    11821 gagatcacca ccctgctcat tcccggcatg aatgacagcg acgccgaact cgccgcgctg
    11881 tgcgcctggg tcgcacagca cctcggcccg gacacgccgc tgcacttttc ggcattccat
    11941 cccgactaca agctggccga cacacccgcc acaccgctcg cgacactggt gcgcgcgcgc
    12001 gacatcgccc tgcgcgccgg gctgcaccac gtctacaccg gcaacgtgca ccacgctgaa
    12061 ggcggtacca cgttctgccc acactgccat gcggcactga tcgcacgtga cggctaccgc
    12121 atccgtgact atcgactgga tgccaccggc cgctgccccg actgccaaca gatcatcgcc
    12181 ggccggttcg atccgaaacc ggggcggttc ggcaaccgcc gcatcccgat ttcgattcgc
    12241 ccgacccact gacgttcacg cttcgcccag caaacggatc acctctgcgt cttcgacctg
    12301 gggaaacgca gcataatatt gcccgaccgc ctgaaaatcg gacggcgttt ccagacacag
    12361 cacttcgtcg gcatgacgcc ggacttcgat cagggtatcg ggcggcgcca ccggcacggc
    12421 acagaccagc cgcgccggat tgccggcacg caccgcatgc agggcggcaa tcatggtaaa
    12481 cccggtcgcc agcccgtcat cgaccacgat caccacgcgt cccgccgggt tgatggcggg
    12541 acgcagcggc gtgtaagctt cgcgccgccg cttcagcgtt tcaagctggc gcagcttctc
    12601 gcgttcgagg tattccgcat tggcacccat gcgcgccgcg taatcggcga tatagctgcc
    12661 gccgttctcg tcaatggcgc caacggcaag ctcctcgttc aacggtgcac gtagcttgcg
    12721 taccagcacc acgtccatct ctccgtccag atgacttgcg atgagcctgg ccatcggtac
    12781 cgccccgcga ggaatggcca agatcagcgg atgctggccg cgataacgtt ccagacgctt
    12841 cgccagccgc ctcgctgcgt gttcacgatc ctgaaacatg atgccctccg ttccgtcaga
    12901 cctgcccacc ctggcaggtt caaagccggt ttcctgctgt ggcgaaaccg gtcacgcccc
    12961 atcctgcacc aggcaccggg gccttctcca caggtcggca cagtggaatt catcgccatg
    13021 ctgccgcgcc agccggccac ggcaacagca cgacacgatc aggattcgcg cttcttgcca
    13081 cggcatggca tggcatgccg acgacagaca tgacaacgcc acgcgctcca gatcataata
    13141 cccctgccgg cggatcgtgg cgcatgcttt gtccggctgt acaaaaaaga caacggacac
    13201 acgacagcag cggaggccgc aaaccaacat gtcgcacacg agcatagcag ggcgagtcct
    13261 gccctggttg atcacgaccg cgctgcggtt gttgtaccgc gtcgaagtgc gcggcgcgga
    13321 acactggcag cacgccgggc agcgcgtgct catcgtcgcc aaccacatgt cgctgctcga
    13381 tgcgccgcta ctcgccgcct tcctccccga agccgcaggc ttcgccatca acacgcaaat
    13441 tgcgcaacgc tggtgggtac gaccgctgct tcatctggtc agagcctacc gtatcgaccc
    13501 gaccaacccg gtcgccgcca gaacactgat cgacgaactg cgccgcggcc gcaggatagt
    13561 gatgtttcct gaaggtcgcc tgagcgtcac cggcggtctg atgaaaatct atgccggccc
    13621 cggcatgatt gccgaccacg caaatgccac gatcctgcca gtccgcatcg acggcacaca
    13681 gttctctccg gcctcaccga tgcaaggcaa gacacgcctg agcctgtttc ccaaagtcac
    13741 actgaccttt ctgccgccaa gcaggctcga cctgccacag cgcctgcgcg gccacccgcg
    13801 acgccaggca gcagccgcgc aactccatgc actgatgtcg cgcatgctgt ttgacagcag
    13861 cccggtgcac gacacactga tgcacaacct gatcgaatcg atgggggtgt atggtggccg
    13921 ccacctcatt gccgaggaca tcgagcgcaa accgctcagc taccgtcgct tcattgcggc
    13981 aagtttcgtg ctggcccgcg ctctgcgtcg tctgacccgg cgcaacgaaa cacgcatcgg
    14041 actgatgctg cccaacctga ccgccaccgc catcagcttt ttcgccatcc agacactcgg
    14101 ccgcacctgc gtgctgctca atttcacggc aggccaggca cagattctcg ccgcctgcgc
    14161 caccacctgt ctcgataccg tactctcgtc gcgccgtttc atcgatgccg ccggtctcga
    14221 tgccgtatcc ggtgctctgc atgcggcagg tgtacgtctg atattccttg aagacctgca
    14281 gcacgatgcg ggctggatgg ccaggctggc cggtctgctg tgcgcatgga tgccgcaaca
    14341 ggcgctgcgc cgccattcac aggctggccc ggatgatccg gcggtcatcc tgttcacctc
    14401 cggttccgaa ggtgcgccaa aaggcgtggt gctaagcagc cgcaacattc tcgccaaccg
    14461 ttaccagatc gccagccgga tcgacttcgg cgcacaggac accgtgttca actgcctgcc
    14521 gatgttccat gccttcggac tcactggcgc gacattgcta ccgttgctgt ccggcgccag
    14581 agtgttcttc tacccatcgc cgttacatta ccgcatcgtt cctgaactgg tatacgactg
    14641 caacgccacc atcctcttcg gcaccgacac ctttctcgcc ggctatgcgc gcaacgccga
    14701 tccatacgat ttccactcgc tgcgctacgt gttcgccggt gcggaaaagt tgcgcgaaga
    14761 cacccgccgc acctggtcgg aaattttcgg gttgcgtatt ttcgagtgct acggcatcac
    14821 cgagaccgcc ccggtactga gcgtcaacac cgccatgcat taccgcgccg gtaccgttgg
    14881 ccagctcttg ccaggcatcg atttccgtct cgaacacatg cccggcatcg acatcggtct
    14941 gcaactgttc gtgcgcggac ccaacgtcat gctcggctac atgaaatccg accatcccgg
    15001 catcctgcag ccgctggaag atggctggta tgacaccggc gatatcgtgc gcatcgatga
    15061 cgatggctat ctgacgctgg tcggccgcac gcggcgtttc gccaaggtcg ccggcgaaat
    15121 ggtctcgctg gccgccgtcg aggcgctgat cgccgaattg tggcccgacg cggcgcacgc
    15181 cgtagtcagc gtgcccgacg cgcgcaaggg cgaacagctg gtatggttca ccgatagcgc
    15241 agatgccggc cttcgcgccg tgcagacggc attccgcacc cgcggcatgt cgccactgac
    15301 gctgccgtca cgcatgcaac ggctggagca gttgccacgg ctggggtcag gcaagacaga
    15361 ttacgtgcaa ctgggagcgc tggcacgcat cactccggct gcgcccgaaa cgcaggagga
    15421 acacccgcca cgcagcacac cgcatcatgc cgaaggcacg gatttacggt aaaatccgtc
    15481 gttgaccttt cacatccttc acttgcacga taccgctcat gagcccgcac aatctgtttc
    15541 attccctgac cgaattcgac cgcggcgacg gatcgcgcgg ccagctgtat tccctgcccg
    15601 cacttgaagc cgccggtctg ggcgccatct cgcgcctgcc cgtttcgctg cgcatcgtgc
    15661 tcgaatcggt attacgcaac gtcgatggca cccggatcac cgaacagcac gtgcgcgaac
    15721 tggccggctg gcaacccacg gccgcacgca gcgaagaaat tccattcacg gtggcacgcg
    15781 tgctgctgca ggactttacc ggcgtgccac tgctcgccga cctggctgcc atgcgtgcag
    15841 cggcccaggc agccggtcgt gacccggggc tcatcgaacc gctggtgccg gtggatatgg
    15901 tcgtcgacca ttctgtacag gtcgacgtat tcaaccgcgt cgatgcagtc gaacagaaca
    15961 tgagcctcga gttcacccgc aaccgggaac gctaccagtt cctcaagtgg ggcatgcaag
    16021 ctttcgatac cttcaaggtg gtgccccccg gaatcggcat cgtgcaccag gtcaacatgg
    16081 aatacctggc acagggcgtc atgtcggcaa acggcatatt ctaccccgac acgctggtcg
    16141 gcaccgattc gcacaccacc atgatcaatg gcctcggcat cgtcgcctgg ggcgtaggcg
    16201 gtatcgaagc tgaagccgcc atgctgggcc agccggtata tttcctcacg cccgacgtcg
    16261 tcggcgtaca cctgaaaggg cgcccggccg cgggcgtcac cgccaccgac gtcgtgctga
    16321 ccctgaccga aatgctgcgg cgcgcgcgcg tggtgggcaa gttcgtcgaa ttcttcggcg
    16381 aaggcgcggc cgcactgtcg cttcccgacc gggccaccat agccaacatg gcgccggaat
    16441 acggcgcaac catgggattt ttccctccgg atgaaaccac ctgcgcctat tacctggcca
    16501 ccggccgcga cccggagcgg atcgctacgc tgcgcaacta tttcactgcc cagggcctgt
    16561 tcggcattcc acaagccggc cagatcgatt actcgcagac gctggagctc gatctcgcca
    16621 gcgtcacgcc atccgtggcc ggaccgcgtc gcccgcaaga ccgtatcgag ctgggccagg
    16681 tcgcgcattc gttcgaccgg ttgctgacaa cagcgaccga taccggcggc tggggaaaat
    16741 ccaccggaac cacacctagc gccaagctca atcacggcga catcgtcatc gccgccatca
    16801 cctcctgcac caacacatcc aatccggggg tgatgctggc cgccggactg ctggccagaa
    16861 aggcggtcgc actcggactg accgtgccgg accacgtcaa gacctcgctc gcgcccgggt
    16921 cgcgcgtggt cgccgactac ctggcagcgg ctggcctgct cgaaccactt gaacagcttg
    16981 gcttcaagct ggttggctat ggctgcacca cctgcattgg caactctggc ccactgccgg
    17041 aggacatcga gcacgagatt ctctcccgcg acctggtggc ggcggccgtg ctctccggca
    17101 accgcaactt cgaagcgcgt gtgcaccaga acgtcaaggc caattatctg atgagccccc
    17161 cgctcgtcgt cgcctgtgcc ctggccggga gcgtacgggt tgacctgaca cgcgaaccgc
    17221 tcgggcacgg acgcgacggc gcaccggtct ggctgcgtga tgtgtggccc agccatgaag
    17281 aaatcgctgc ggtcatgcac catgccaccg acccggccag ctaccgcgca cgctatgccg
    17341 acctgaacgc caacaatccg ttatgggccg ccgttccggc gccgaccggc aaggtatatg
    17401 cctgggatac ggcatcgacc tatatccagc agccgccgtt ctttacactc gacgccacaa
    17461 ccgtgggcga catccggcat gcgcgcgcgc tggcgatttt cggtgattcg gtgaccaccg
    17521 accatatcag cccggccggg tccatccgcg ccgactcgcc ggcaggactc tacctgcagg
    17581 cgcatggcgt gaacaaggcc gatttcaaca gttatggcgc ccggcgcggc aaccacgaag
    17641 tcatggtacg cggcacattt gccaatgtgc gcatccgtaa ccgcatggtg cccggcagcg
    17701 agggcggtgt aacccgttac cagccggacg gcaagaccat gagcatctac gatgccgcca
    17761 tgcaatacgc aacacaccac gtgccgctaa tggtattcgc cggcgaagag tatggcaccg
    17821 gatcctcgcg tgactgggca gccaaaggta cgcgactgct cggcgtgcgc gtggtgatcg
    17881 cccgcagctt cgagcgcatc catcgcgcca acctggtagg catgggcatt ctgccctgcc
    17941 agttccatgc tcgggacagc gcagatagcc tgcagctcga tggcagcgaa cagttcgacc
    18001 tgttcggcct ggaaaacggc atcacgccgc aacaggaagt gctgctgtcg atccggcgca
    18061 gcgacggccg tgtcgacacc gtgtcgctga cgctgcgcat cgacacgccg atcgaggtgg
    18121 actattaccg tcaaggtggc attctgccgt tcgtgttgcg ccagctgagc agaaactgat
    18181 cgcatgtccg agctcaacga cccgcgcgta ttctttgccg ccgaacgcac cctgcttgcg
    18241 tggaaccgca ccagcctgac gctgatgggt ttcggtttcg tggtggaaaa attcgcgctg
    18301 ttcgtccgca tgctgatgcc aggcagccag actgccgcca caacgcacgg cccctctttc
    18361 tggatcggca tcagcttcat cgtgctcggc gcagctaccg cattgctggc aaccctgcaa
    18421 taccggggtg tgctggccac cttgaggcca gtcgaaattc caccaggcta ctggacgcat
    18481 ctggcggtgt atgccaccct gttgctcgcg gtactcggca ttgtgctggc cgcattcctt
    18541 gccaccgacc tgaaataacc ttccattcca gttactgcga gccatgaccc gaaccattct
    18601 cgtcacatcc gccttgccct atgccaacgg cgccatccac ctcggccacc tgatcgaaca
    18661 catccagacc gatatctggg tacgctatca caagatgcgc ggccacgact gccactatgt
    18721 ttgcgctgac gatacgcacg gcacgccgat catgctgcgc gcccaggccg agggtatcag
    18781 cgccgaaacg ctcatcgagc gcgtgcatgc cgaacacctg cgcgattttc gcggttttca
    18841 tatcgaacac gacagctacc attcgaccca ttcagaagaa aaccgcacgc tggtcagcga
    18901 agtctacctg cggctgcgcg atgccggact gatcgagcgc aagaccatcg agcagtatta
    18961 tgatgccagc cgcgaaatgt tcctgcccga ccgcttcatc aaggggcagt gtccgcgctg
    19021 cggcgcagcc gaccagtatg gcgacaactg tgaagtctgt ggcgccacct attcgcccac
    19081 cgacctgatc aacccggtct cggcactgtc tggcacacct ccggtgcgcc gcgagtcgga
    19141 acacctgttc ttccgcctcg gcgcatgcac ggatttcctg cagcagtgga cccggtcagg
    19201 cgcgctgcaa cgcgaagccg ccaacaaact cgatgaatgg ttctctgccg gcctgcagaa
    19261 ctgggacatc tcgcgcgatg cgccctattt cggttttgag attcccgacg ccccaggcaa
    19321 atatttttat gtctggctcg atgcgccggt cggctacatg gccagttcgc tgcatcttgc
    19381 ccgcgaacgc ggactggatt tcgacacctg gtggcgcgcc gacagccctg ccgaactgta
    19441 ccatttcatt ggcaaggaca tcctgtattt tcatgcgttg ttctggccgg ccatgctgca
    19501 ttattcgaag catcgcaccc ccaacgggct gttcgtgcac ggctttctca ccgtcaacgg
    19561 cgccaagatg tccaaatcgc gtggcacgtt cattaccgca tcgagttacc tcgattgcgg
    19621 actcaatccc gagtggctgc gctactactt tgccggcaaa ctcaacggca gcatggagga
    19681 catcgacctc aacctgcagg acttcatcgc ccgcgtcaac agcgacctgg tcggaaaatt
    19741 cgtcaacatc gccagccgca cggcaggctt catcgtcaaa cgcttcaacg gccgtctcgc
    19801 cgaccgtctg ccggagttgc cgctgctaaa tgagctgcaa ctggccagcg acaccatcgc
    19861 ccgccactat gaatccctgg attattcgca tgccatgcgc gaaatcatgc ggctggccga
    19921 cctcgccaac cagtatgtca acgaacgcaa accctgggaa atcgccaaac aggcagagaa
    19981 cgagcacgac ctgcatgaag cctgcagcgt cagcctcaac ctgttccggc tgctggcgtt
    20041 gtacctcaag ccggtattgc ctgcgcttgc aggacaggta gaggcctttc tcgacatccc
    20101 gccgctgcaa tggacagacg cacagcgctg gctgaccggc catgtcatcc gcccctatca
    20161 gcatctcatg gcgcgcgtgg aacagggcca gatcgatgcg atgctcgccg ccaacactgc
    20221 ggccgcggtc gcaacgcccg gtgcggccag cgtcattcct gctgccgctg tcagcacgac
    20281 caccagcaca gccattccgg ctgccccggt cgaagaatcg acgctgatca atgtggatga
    20341 tttcgccagg gtcgatctgc gcattgcgcg catcgttcgg gccgagcacg tcgatggcgc
    20401 agaaaagctg ttacgcctca cgctcgatgt tggcgaaccc gaaccgcgca acgtgttcgc
    20461 cggaatcaag tcggcctacg atccagccag cctcgaaggc cgtctgaccg tcatggtggc
    20521 caacctcgcg ccacgcaaga tgaaattcgg catctcgcag ggcatggtgc tcgccgcttc
    20581 gggcgatgcg ccagggctgt tcctgctgtc gccggattcg ggtgcccaac cgggcatgcg
    20641 ggtcaaataa cccttgcccg aaccgactgc catgttcgaa gaccaactga tccggatact
    20701 gacgcgtttc aatcgcctgc tgcacgttgc tctggcggta gcgctggtca ttgccagcgg
    20761 gctggtgatc tgggagttca gcgtcgaggt cagcagtgcc gtgctgcaca accacctggt
    20821 caaaggattc ctgcaatcgc tgggcacgct gtttctggtc tggacgctct ccagcctgat
    20881 ttcagcggag atcagctatc tgcatgacgg cattttccag gtgcgtatct ttatcgaagt
    20941 cgccatcatc acactgctgc ggcagatcat caccaaaccg gtgctgatcg ccaccggccc
    21001 cggagttccg gacgatgctt tcgagccgat gcagtacggg ctgatcctgg cagcactgct
    21061 ggtggccggc atcctgcacc ggctggtcgg gcgcacaccg cctcccgggg tacgcagccg
    21121 cgacaacacg ccgccacccg gttcctgagc cggaacggct gcgcctcagc gcgaggcagg
    21181 cccgccgcgg aacagggaac ggatgcgtcc gaccagcccg gcatctgccg gccgcggcgg
    21241 cacatcgacg cgatgccgcg cagccacttc cggatgaaga tggatctcgc gcgccagttc
    21301 catgcctgac tggatgccgc gcagcatcat cgccttgcgc atcggcttgg gcagaaaacg
    21361 gtgcacctgc ccctgattga tcaattccac cagcagaccg gtatcgcgaa acgtggtttg
    21421 cacgatggtc acgatatgcg ggtgatgcag cttgagcagc ttcaaaaacg gcatcatgtc
    21481 atccccgccc acactcagtt cggaaatcac catcgaaggc tggttacgtt cgaggatgcc
    21541 catggcatca ggcagacttg tgacccagtc gagcgcgaat gcgccggaga acccttcttc
    21601 gatcatggtg cgcagcccgc tggccacatc ggccgcatcg tcgatgagca ggatgcggcg
    21661 ttgcgcggtc tcgccgcgaa gcggtactgc cggtgccagg gtgcgcaggc caacggcaat
    21721 ggccgccgct tcgcccacga tgctgcgcaa ctcgtccgca ttccatggct tgttgatgta
    21781 gcgaaatact tcgccttcgt tgatcgaatc gatgatggct tcgagatcgg aatacccggt
    21841 cagcagcaga cgcatggtat cgggcgacag gtcgcgtgcc ttgcgcagca ggtccacgcc
    21901 ctgcatgatc ggcatgcgct ggtcgctcac cagcacgtgc acggtttcgc gtttgagcat
    21961 caccagcgct tcgcgcggat ccgtcgtcat cagcaccttg aactgcgtgg cgaacagcat
    22021 gcgcaacgag cgcaggatac gctgttcatc atccacgaac agcacggtcg caggcatggg
    22081 gccggccggg aaagacgcgg gtgttgcact ctgcatgcga tgctcctcat tcatgcgccg
    22141 gatgtcaccg gcaggctcac gcaaaaggcc gttccttttc cgggtttcga tttgacgcga
    22201 atatggccgc cgtgatcgcg cacgatcttg tagctgatcg acagccccag tccggtccct
    22261 tcccccaccg gtttggtggt gaagaacgga tcgaaaatcc tttccagatt ctccgcgcgg
    22321 atgcccttgc catcgtcctt gaccagcacg tgtacaaatc caccatggca gaccgtgctg
    22381 attcggatgc ggccatgctc ttcgatggcc tgagccgcat tgctcaccag attcagcaac
    22441 acctggttga tctgtgacgg cgcacaggtc actggcggca actcgcccag gtcgcggatc
    22501 acttccactt tgtgcttgag caggttgtgc gcgatgagca acgtactttc gacgcattgg
    22561 ttgatatcga cattgactac agccgaacga tcgagacggc tgaaattgcg cagattgatc
    22621 accagatcgg aaatctgttc cagcccatac atcgtgtcag taaacagcgc ttccatgtcc
    22681 tcgggtgcaa atccggccgc cgcctgtccg cgcacggcca tcgctcgctc gaatgcctgc
    22741 tgcagcgcag cgtcgtcggc ctgcggcgac tggagcagca tcaccaggct gtcgaacacc
    22801 tcctgcattt cgcgcaactg accaaacgcc gaatgcgcca gttcgacatt gttgcgcacg
    22861 tagcccagcg gtgtattgat ctcgtgcgcc aatccggcca ccatctggcc gagcgacgcc
    22921 attttttccg actgcacgag ttgcgtctgc gattcctgca accggttcag tgattgcgcc
    22981 aacgaatcat tgcgcgccat caggcgatct tccagaccgc gcagcagatc gatgaccgag
    23041 cccacaccat ggtattcacc atcacgcgtg acaatgaaat cttccacgat cgggaaacgg
    23101 atattgcggg tgatatggcg gctggcctgt tccaccgaat gctcgagcgg caccaccagc
    23161 ggcgcatcgt tcatgaacgc aacgacaggc ttggctccct gcagttcgcg gccgtaacgt
    23221 cccatcagca ccttctgcac gccatagcgg ctgatcacac cgatcggctg gccgtcgcgc
    23281 actaccggca gcgacagaaa accgtcataa cgcggttcca gaaacagatc gccgacctcc
    23341 atgatggata cctcgggatc gagcggttcc acgttcacac tcagttcggc aacatgctcc
    23401 actcaccacc tgccgtagca ttcacaacgt tgagcggcga tgctagcaaa agaatatgac
    23461 agattctgtg tccggaaact gacaatccgg tatggcacta tccggctcag gcggccatga
    23521 tctgtgcaac gtcgaaacgc cagacatccg ggtcttccac gccgttcacg gcatcacctg
    23581 ccagcgaaag atcgatctgt tccatgcgga tgcgggcatt ggacagcgtg gaaaaaaaca
    23641 ccttgaccac cggcaacagc aggctttttg ccgactgccc caccaccacc cccaacctgc
    23701 cggatttcag tctgaccagc gtacccaccg ggtagatgcc caccgtcttg acgaatgcct
    23761 tgaatatccg gtcatcgaaa tggccatcct gccagcccgc catcttgcgc aacgcatcgg
    23821 ccggggccca gccggccttg tagcagcggt tcgacgtcac cgcgtcgtac acatcgcaca
    23881 ctgcgcccat gcgagcgtac agactcagcg cctcgccgga cagtccgtcc ggataacccg
    23941 tcccgtcaac acgctcgtgg tggtggcggc acacgtcaag cacgatttcg tcctcgatct
    24001 gcgtcttccg caacaggtcc caaccccgct cgggatgtga tttgatgatt gcgaattctt
    24061 cgtcagtcag acgccccggt ttgtccagca cctgcgatgg aatggccatc ttgccgacat
    24121 catgcagcag gccggccatg ccgagcgttc tggaaagctg ttgtgacagc ccaagctgac
    24181 gcccgagcgc cagcatgagc gcgcataccg ccaccgaatg caggtaggtg taatcatcct
    24241 tgtccttgag ccgtaccaga ctcagcagcg ccccagaatt gcgcgctacc gactggtcga
    24301 tctcatccac cagtccggcg atggccccgg tcggcaaggc attgcccatg cgcgcttcgg
    24361 caaacatgtc catgactgcc tgcttcgcac gcgcctgaac gacgcgcgcc cgcgccagtt
    24421 cgccttccag gctggtctgc tgcggccggg ccggttccgg ttcgaccgcc tgcaacagca
    24481 ccgcttcggt gtactgttca gtggccgcct cgtcgtcata ctccggcggg atcagcagat
    24541 ccagaccacg tgtggtatcg atccacacat cgcgcaccgc gctcttgcgc agcgtgtcca
    24601 gatcgccggc atcttccagc aggaacgacg ttctccagaa cgggtgatcc atccagctgc
    24661 cgcacaattc atcgatatac attcccagac gtacatcgct taccttgact tttctgcgca
    24721 tgactcatcc tccaccggcc accctctacg acagtccgcg gcatttccgg catgccgctt
    24781 attattttat tgtgtcctgc accagtcgcg gatcagcaat tgcacattcg cctggtcctg
    24841 atactggttc cagtccagct ggtacagggc ctcgatccgt tccggcaagg tatcgctgca
    24901 ttgaaacaga atggcatcga actgccggtc accacacccc agcaccagct tgagatgccg
    24961 ctcgcggatc acccgctggc gcaacacgcg gaaactgttg acaaactgcg gcatggggaa
    25021 cccctgtccc cacacctggg catggattgc ctgcgccagt tcgggcgcca tctcctcggg
    25081 ttgcaggctg ccatcggtga gaatctccgg gtgcagatcc tcgggcacca gcatgctgcg
    25141 caccgccgac tcgaacgccg ccacaaaatc cgtcagccgc gcttcgcgca acgtcagtcc
    25201 ggcagccgcg gcatgtccgc caaatttttc aatcagcccc ggatgtgcgt tcgccacccg
    25261 atccagcgca tcgcgcaggt gcagcgcagc aatggaacgg cccgaaccgc gaatttcacc
    25321 ttcccccgcg cgcgcaaaac agatcaccgg gcgatggaaa cgttccttga gacgggaagc
    25381 cagaataccg accacgccag catgccagtc cggctcgaac agcaccagac tgtaaccggg
    25441 cgcatcctcc agttccggca gtgcagccat ggcctgctgc tgcatcgaat cctcgatctc
    25501 gcggcgttcc tgattgagtt gctgcagctg ggaggccagc gacatggcct gcgcctcgtc
    25561 atcgctcagc agacaggcga taccaagcga catgtcatcg agccggccgg cggcgttaag
    25621 gcgtggtccg atcgaaaatc cgaggtcgcc ggcgctcgcg gtggccggat tgcgcccggc
    25681 tgcagcgaac agcgcacgca cacccggaca gccacgccct tcgcggatgc gtcgcaaccc
    25741 ttgtgcgacc agtatgcggt tattgtcatc gagtcgcacc acatcggcca cagtgcccag
    25801 cgccaccaga tcgagcaatg cggcgagccg gggcggggca gccgtgccga acgcacctgc
    25861 cagttgcagc tgtctgcgtg tggcgagcag cacgtagaac atcacgccca ctccggcaag
    25921 gtgtttactg ggaaacgcac accccggctg gctgggatcg acaatcaggc aatccggcag
    25981 gctggtgccg ggcaggtggt gatcggtcac cagcacttcg acgccggccg catgcgcggc
    26041 ctgcacgccc tcgatgctgg cgataccgtt gtcgactgtc acgatcagtt cagcctgacg
    26101 ttcgcaggca atgcgcacga tttccggggt cagtccgtag ccatgaatga aacggttggg
    26161 aacccagtag tcggccttca gaccaaacag ccccaggccg cgcacggcaa ctgcgcacgc
    26221 cgtggcgcca tcggcatcgt agtcggcaac caccagcacg cgctgctggc gcatcaccgc
    26281 ttcggcaagc cgcatcgcag cagcatcgat ccccttgagc gtgccgtaat gcagcaaacc
    26341 ggccagatcc acttccatct gttccgggcg atcaacaccg cgcgctgcgc aaatgcgcgc
    26401 cagcacgggg tgcatgcccc ctgcggccag ttgactcacc gcctgcgcgc cgtatggacg
    26461 ctcacggatt tgttctcgtt tgagtgacac agcattctcc gcactaccgg gatgattgta
    26521 gcgtgagtat gcggtgttgg cacagggatt ccactgctgg acatcccact gtcgccctgc
    26581 ggactccgat cgccgcatat catggttttc taccctgctg ttggcgtata attccacggt
    26641 tcccggcatg tccggatgga gtcggcatga gaccgcagca gacatccgca aaaaaatccg
    26701 cgcaacaagg cggaaaagcc cggcacatca ataccggaaa acagcaggac accatcgcca
    26761 cgcacgtact cgatgtcggc gcacctcgcg atgaaaacgc gcagggtcag gtgctggacc
    26821 ggttgcttgc ccgacagacg gtgcatgacg cgcaaggccg catcctggct acggctttcg
    26881 aaccacgcgt tcgcctttcc ggtaccaccc cggaattgcg gctcgcagac gacaccacgc
    26941 tgatcgccgc gctgtacaac ctgacggaaa atgaacgcac gccgcgctac cccctgctcg
    27001 cgtcgatcga ccagcattcg ctcgccctgc gcgacatcga ctacctgccg gcaccgcacg
    27061 tcattttttc tctccatgca gcatcggatc ccgacaaccc gttggtagcg ctgatcaatc
    27121 gccggctcaa gagcggtttc cgcctgttgc tcgaacagtc cgacgatgcg ccggtcccca
    27181 tgcgcctgat cggcgtcgtg cgcgaagccc gtatcgacac ccgcgaattc agcgcgccgc
    27241 gcctggaaca gaaaatcatc cagttgcgtc atttcggtat ccagcgcatc cacgccgccc
    27301 acattgactg ccaggaaatg ctgggacact gtatcaagct caagctcgac tcctatcagg
    27361 gcgatgcctg caaccagctg acgccacgcg gcctgccgag tatcggcatc aaccggctca
    27421 aggtgctcga actgattgat ctggctgtgg ccggcaaacc accacgggaa atcgccgccc
    27481 atctgcgcag cgatgcgcgg ctgacctatc agatgcttgc ctgtgcaagc cgcaccgtcg
    27541 gcgatggcgc tccggccgcc tcagtcgaac aggtcgcgga aggttatggc atgcagaaac
    27601 tgtatgactg gctcatcctg ctgttgcgca actgcaccgc accggatgct gccgcgcagc
    27661 agacattgcg ccgcagcctg agccgcgctt gcttcctcga cacgctggcg aaaaagtcat
    27721 tgctcggatt ccccgccgat tcggcctggc tgattggcct gctctcgctg actggcgaac
    27781 tgctgcaaca accccctctg caatgcctgg caccgctgcg tctcgacgaa aatatccgcc
    27841 tcgcgctgct ggagcacaag ggtgtgggcg gcatgttgct ggaactggcc gtcgccaccg
    27901 aaaacgccga tcatcatgcg atcgaacacc tggccgcacg ttgtctggtc aatatcgagg
    27961 acgtcaacct ggccatggtc aatgcgctgg tactggcaga atcgacgcca ttctgactgc
    28021 atgcccaccc aatacacccg agccgcaccg ggtcggtcaa tgacgccgaa ttgccaaaac
    28081 cgccagcata tcgcgcacgc gccatcatcg gggtgcggat gttccacgga cgctggacgg
    28141 ttgccacggt tcacgccgtt cctgcccgtc gcgtttcgcg caaactactc gcgcaatggc
    28201 ccggctgcaa cgggccgctg ggccacgaac cccgccacag ccgcgatcac ctcgtccagc
    28261 gcatgtgccg acaggcaatc aatctgttgc agcccgtgca tgccgcgcaa ccaggtcatc
    28321 tgccgcttgg ccagctgacg tgtggcatag atgccgcggt ctcgcagttc ttcgcgcccg
    28381 tattcaccct cgagacaggc ccacgcctgt cggtaaccga cgcagcgcat cgacggcagt
    28441 tcggcatgca acgcatatcg actgcgcagt gcttccagtt ctgccaccaa cccatcggca
    28501 agcatgccga cgaagcgccg tccgatacgc tcgtgcaact gcgcccgatc ggccggcatc
    28561 agcgccagtg gcaaccaatg catcggctgc cgtggcgcac gcggttcatc ccagaacgaa
    28621 gacatcgggc gacctgtcag gcgcaggatt tccagcgcac gctggatgcg ctgagcatcg
    28681 ctgttgctga tgcgggctgc cgcgagcgga tcagcagcgg ccagttcggc atgcaatgcc
    28741 gcccaaccac gctgactggc ctgtgcatcg atctcggcac gcaaagcggc atctgccgga
    28801 ggaaaatcgc tcaacccttc gcgcaacgcc cggaaataca gcatggtacc gcccaccagc
    28861 agcggaatgc ggccgcgcgc cgcgattgcc tgcatcagag gccatgcctg ctcgcagaac
    28921 tgcgccgccg agtagctctg gtcggggtcg atgcaatcga tgaggtgatg cggcgcgcgc
    28981 gcgagcgtcg ccgcatcggg tttggcggta ccgatgtcca tgtcgcggta gaccatggcc
    29041 gaatcgacgc tgatgatttc cagcgggaaa cgttgcgcga gcgccacggc aacgtcggtt
    29101 ttgccgcacg ccgtcggccc catcagcatg atggcgcgcc ctgcagcgga tcgcgccgta
    29161 tccatcacat tcattgcgct gctgccggtt gctccttgcg aaaaaaccgg accctgtttt
    29221 acacttggcc attatactgc ttacctgcat tttccgagac caccatgatt tcgtcgaccg
    29281 gca
//
LOCUS       DAIANL010000092        20015 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_931134, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000092 DAIANL010000000
VERSION     DAIANL010000092.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 20015)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20015)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20015
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931134"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3393 a         5811 c         6855 g         3956 t
ORIGIN      
        1 gtttcctgga cgacggtggc gttcgaccgg ccagtggcaa gcgtatatac ctcgggttcg
       61 accggccggc cgcaatcaca catcaaaagt tttggccggc tgtgtcaggg cgccgtggcg
      121 gaggccgcgc gcatccgcga acagaccggt gtggactgca acgtgctggg cacggtgccg
      181 atccagcaca tgtacggact ggaatccact gtactcatgc cgttgctggg cggtggccgg
      241 ctgacctcgc gccagccgtt ttttccggcc gatattgcgg ccgcactgta cgagctgccc
      301 gagccgcgcc tgctggtgag cacgccgtac catctgcgca agctggtgga atcgggcctg
      361 accctgccgc cggtggcggg tgttctgtcg gccaccgcgc cgctcgagcg agacctggct
      421 gtgcgcatcg aacaggtgct ggatgccccg ctgctcgaaa tctacggttc aacggaaacc
      481 gggcagatgg cgtcgcgacg cagtgcgttg caggatacct ggcggttgtt tcctggtgtc
      541 gtgctgcgtc agtatgctgg cgccaccgtg gcgagcggtg ggcatctgga agaggaacgg
      601 atgctcggcg atcacgtcga gttcgttgcc ccggaagagt tccgactgct gggtcggcac
      661 tcggacatga tcaacattgc cggcaaacgc aattcgcttc agtttctcaa tcatgtgctg
      721 atgcagatcc ccggggttga agacggcgtg ttttgtctgc cgcagatggg tacggcggac
      781 ggcgtctccc ggctggccgc gtttgtggtg gcgccgggac tggatgcaat tacgctgcag
      841 gcggcattgc tgacgcagat tgacccggtg ttcctgcccc gtccgctggt attcgtagag
      901 gcgctgccgc gcgatcgcaa cggcaagctc actgcggcaa gcctggccgg gttgctggcg
      961 caacatgcag cgtgcggagc gtgacatgcc ggtatcgcca ctcagcattg acagcaatca
     1021 tccggcgttt gccgggcatt tccccagtca tccgatcgtg ccaggtgtcc tgttgctcga
     1081 tctggccgga caggcgatcg aggccgcatc cggacggagg gtgtgcgggc tggcggtagc
     1141 caagttcgtc gccgtggcgg agcctggcga tgcgctccaa ctggaatacg aattgcagga
     1201 caacgctgtg ccgttccgca tcctcagcgg tgaacggctg attgcgaccg ggcggttcca
     1261 cctgggcgaa gaggccgcgt catgactgct gcaggcaagc gcgtcacggt cggccggatg
     1321 ccgggatgga tgcgcagccg ggaacgcggc agcttgttct ggctgcgcgt gatgagcctg
     1381 atttcgctgg cgttcgggcg acgcgtttcg cggttggcgc tggggttcgg tgccgcctat
     1441 ttcgtgcttg ctgccgggga tgcgcggcgt gcctcgcgcg cctggctgca gcgggcgctg
     1501 cgccgtccgg tcggactgcg cgatgtgtat cgtcatatgc gtgctttcgc ctgcaccatt
     1561 cacgaccggg tctatttgct caatgaccgc catgcgctgt ttgatgtgcg cgtcatgggc
     1621 ggcgaggcgc tgcgcggtcg taatctgggt gggcaaggct gtttcctgtt tggcgcgcac
     1681 ctgggcagct tcgaggtgtt gcgtgcgctg gcgcggaacc agccgcagct caaggtgtcg
     1741 atcgccatgt atccaggcaa cgcacaacgg atacagtcgg tgctggcggc gatcaatccg
     1801 caactggtac aggatatcat tgccattggt cagctcgatt cgatgctgac cgtgcgcgat
     1861 cgtatctgtg atggtgcgct ggtcggcatt ctggcagatc gggcgagtgg gccggaccag
     1921 taccttaccc tgccatttct cggtgaaccg gcggaatttc ccacaggacc gttccgcatg
     1981 gcggtcatgc tgggccatcc ggtgtatttc atggcaggcc tgtaccgcgg gggcaatcgg
     2041 tatgacgtgc atttcgaact gctggacggg gcggttggtg ccgtaacgcg cgaagccgca
     2101 gtcgaggcgc tgatgcgacg ctatgtcgaa gtgctcgaac ggcacagcct ggacgcaccg
     2161 tacaactggt tcaatttttt cgatttctgg gagcggcact gagatgcgcg gaatcaccca
     2221 ctggcgggcg gtgctggcgc tggcgctggt gtttacagca ctgccggccg gtgccgatcc
     2281 gctgcaactg cccgaactga tgcaccggct ggccggggtc aaggcgcgac gggcacattt
     2341 tgtcgaagaa aggcacatcg gtgtgcttga tgtgccgctg cattcgttgg gcgaactggt
     2401 ctatgaagcg ccggatcggc tggaaaagcg cacgctgcaa ccgcggcccg aagacttgag
     2461 gctcgatggc gatatattga ccatcgtacg gccggacaag caaccggtga cgatcgacat
     2521 ccgcaattac ccacaggcgg cggcactgat tgccagtgtg cgcggtacgc tggccggcga
     2581 tgtcgctgcg ctggagcagt tctaccgcat cgaactgcac ggcacgctga ctgcctggca
     2641 gctgcaactg gtgccacggg acgggcagat gcgcgacctg ttcagcgaca tcgagatcga
     2701 tggcgaacgt gacgacgtac gtctgatccg ctatgaccag cccaatggtg accatgcggt
     2761 gatgaccatc gcccgggatg cggaacgatg aaacaccctg gccgctctgg cgtgcttgcc
     2821 atctgggccg gactgttggt gttggggctt gtcctggtcg ctcaggcgcg ctatgtcgcc
     2881 gatctttcgg cgttcatgcc gcgcgcgccg gatgcgaggc agcagatgct gctcgatcag
     2941 ctgcgcgatg gcgctgtggc gcgcatcgtc atgatcggta tcgagggtgg tgatgccgca
     3001 gcgcgggcaa agctgtcgcg ccggatggct gccggtctgc gcgccagcgg ccagttcgtc
     3061 ggcgtgctga acggtgaatc ggcgcagcag gcgcgagatg cggcctggct gttcagtcat
     3121 cgttatgtgc tcagtccggc catcagacca gaacgtttta gcctggccgg gctacatgcc
     3181 gcagtggcca gcagcatcga cagcctgtcc ggtgatatgg gtacattgac cgcacaatgg
     3241 ttcgcgcgtg atccgaccgg tgagatgcag gtgctcgcgg agcgtttttc tgctcacgcc
     3301 cagccttcga gcgaggatgg ggtctgggta tcgcgtgacg atgcgcgcgc cgtgctgttg
     3361 gcctacacca cggcggcggg ttccgatacc gaagcccagg cgcgggcact ggctgaaatc
     3421 cgccgcgagt tttccgcgct ggggcctgca gcgcagggta cccggctggt gatgagcggt
     3481 acgggagtgc tgtcggtagc ctcgcgtaat accattcagg gtaccgtaac ccggctggcg
     3541 tcggtcagtc tgttgctggt ggtggccatt ctgttcagcg tgtaccgttc gccacgactg
     3601 ctgctggccg ggttgttgcc ggtgttgtcc ggtgccgtgg ccggtattgc catggtcagc
     3661 ctgttttttg ctcaggtgca tgcgttgacg ctcgggttcg gcacgacgct gatcggcgaa
     3721 gccgtcgact attcgatcta ttttttcctg cagcgcggcg tgatgcgcga ggaagcgcat
     3781 ttctggcgga cgctctggct gggtgtgctg acctcgctgg ccgggttcgc tgccttgctg
     3841 ttttcagcgt ttcccggact ggcgcaactg ggactgtatt cggtcagcgg gctgactgtc
     3901 gcggcgctgg tgacgcgttt cgtgctgcca cagatcgttg cgccggattc ggtgcgtctg
     3961 cgcgatctga cccaggctgg tgtcaggctc gatcggttca tcgaccgtgc ggcaggactg
     4021 cgctggttgc tgatattgct ggtggtgggc gcctgcgttg tcgtcgtgac acacggtggc
     4081 gggatatgga accgccagtt gtctgcgctc agcccggtgt ctcgcgccga tcagcaactg
     4141 gatgcacaat tgcgcagcga catgggtgct ccggatgcgc gctatttcgt ggcgatcggc
     4201 gcagccgatg ccgaacatgc gctgcagcag gccgagcgtg tcgacccgct attgcagcga
     4261 ctggtggctg ccagggtgat cgccagttat gattcgccgg cctgggtgct gccgagccag
     4321 aagctgcagc gctggcgtca ggcggcgttg cccgatgcgg tgcaggctcc gggcctgctt
     4381 acggcggcgc tgcatggtct gcccgtgcag gccaaccggc tcgatggttt cctggccgac
     4441 ctggaagcgg cgcggaccgc tcccgtgctg acacgcgccg acctgaccgg aacatcgttc
     4501 gccatgctgt atgacaccat gttcatcacc cggccagggg gcgtgctgtt actgattccg
     4561 ctgcaaccgc cggcaggtgc gcacggtgag attgccatac cccgcatcga tgcggccctg
     4621 aatgcagcca gtctgcgtgg catcgttgtc atggacctgt tggccgaaac ttctggcata
     4681 ttcaacagtt atctgcatga ggcattgttg ctgtgcgcct ttggtgcgct ggccatctgt
     4741 gtgctgctgt ttgctgcctt gcgccggata cggcgggtag cgcgtatcct gctgccgctg
     4801 gtggctacgg tggtctgcgt gacaggactg ttgctcgcaa gcggggtgca gttgacgatc
     4861 ctgcacctga ttggtttgct gctcacggtg gccattggct cgaactacgc gctattcttc
     4921 gaacgtgacc cggcgctgcc ggacagcgac gccgaacggc gccagcgcca actgtcgctg
     4981 gtggtcgcca atctgacaac cgtgtgcagc ttcagtctgc tggggttttc cggggttccg
     5041 gtgctgtcct acatcggtgg cacggtcggg atgggaacct ggctggcgct ggtgtttgct
     5101 gcggtgttta cgcgcaggaa gtccgatgca attgctgttt gatcaggccg atgcgtgcca
     5161 ccgcgccgag acgctgctcg tctggttgcc gcctgcgggc gcccggccgg aagcgttcgt
     5221 cgaacagggg ttcgtggctg ccctgcgggc ggcggccttg ccggtcgact gcgcgttggc
     5281 tggtgtcgat gatcgttggg cgatggattg cagcgcgcct gccgtggtgc acgagcaggt
     5341 gattcggccc gcgcgggcgg cgggctatcg ccagatctgg ttggcgggca tctcgcttgg
     5401 tgcgttcaat gcgttgcatt acgcggccag acacgcggcc tgcctggccg ggatgttcct
     5461 gttggcaccc tatccgggga cacgcgacat cctgctcgaa atccgtgcgg cgggcggccc
     5521 gcaggcctgg gccacgagcg caccggcaga catgcgcgat gagcgcatct ggtggcattg
     5581 gctggtgcag cagtcgcatc atgatgaatg gcctgtgccg gtgtggtatg ccagtggtga
     5641 cgcggaccgg tttgcagcag ggcaggcgtt gctggcggag ttgctgccgc agcagcgggt
     5701 gcggcacggc ccgggcggcc acgaatgggc ggtctggctg cggctgtggc gggaaactct
     5761 tgccgagggc aggctgtttc aggcggcggg aaggacgcaa ccatgaggtc ggtggggctg
     5821 tcgcggccgg ccgtgctggc tgtggccagc atcggggtac atggtgccgc tgcggcgctg
     5881 ttgctggtcg acgtcacgtg gtggccgtgg gcggccgcga cgctggtgct gaaccacgcg
     5941 ctcatcgcgg cggctggcct gtgcccgcgg tgtaactggc tcgggcccaa tctggtgcgc
     6001 ttgcctgaag cctgcgtgcg ccggagtgag gtggcgctca ctttcgacga tggccccgac
     6061 ccggaagtga cgccactggt gctggatata ctggcggcgc atggcgtgcg cgcgactttt
     6121 ttctgtattg gcgagcgcgc gcagcgctgg ccgcagttgg tgcagcgtat ggtggccgaa
     6181 ggccatcggg tggaaaacca tggccagcgt catccggtgc tggcggccat gttcatgccg
     6241 gcaggttggc gtgccgaggt ggcggacggc gcacgcacgc tgacccggct ctgcgggcgg
     6301 ccgccgcgct attaccgcgc agtggccggg ctgcgtaacc cgttcctgca accggtgttg
     6361 cgcaggcagc gcctgcaact ggccggctgg tcgcgacggg gctacgatac acgtagccgc
     6421 gatcccgggc gggtgctgca acggctcacc cgcggtctgg ctgcgggtga cattctgctg
     6481 ctgcatgatg ggcacgcggc tcgcacgcgt acgggacggc ccgtggtact ggaggtgctg
     6541 ccgcagttgc ttgcgtggct ggccgggcgc gggctgcgtg ccgtgccttt gcccgagccg
     6601 gaatgttgaa cccgcaacgg gatgtgcctg ttgcagactg gtgggtgttg catcgatcag
     6661 tctggtaaaa tgccctgctg actacagact gactgtcttt tcttcatgac gggttcgatg
     6721 tgacaccatt gcaactcact gcctatacca tgaccagtgc gctgggcgcg gggcttgagc
     6781 cgacgctcga tgcactgcgt gcgcggcgca gtggcatcat gtgcaaacac tgggaaacgg
     6841 tcgaagccgg gttgtgcatc ggcgaagtgg ccggagtgga tgacgtggtg atgcgcgctg
     6901 atctggcgca ctatgcctgt cgcaataacc ggctggcaca acttggcctg gagcaggatc
     6961 agttcgccgc ggcggtcggg gccgccacca gtcgctatgg ccgccgccgt atcggtgtgt
     7021 tccttggcac cagcacttcc ggcattctca gctccgaaat cgcttaccgc caacgtgatc
     7081 cgcacagcgg tgcactgccg gccagccatg attaccggca tacccacaac agttattcgg
     7141 tcgcggcatt cgtgcgcgaa tattttggct tgcagggacc ggcgttcgtc gtgtcgacgg
     7201 cctgttcgtc gagcgccaag gtatttggca acgcctggcg catgattgca ggcgggtgga
     7261 tcgatgccgc gctggtaggt ggtgtggatt cgctgtgcct gaccacgctg tacggctttc
     7321 gttcgttgca actggtgtca gaccaaccgt gccggccgtt cgatgctgaa cgcaacggcc
     7381 tgtcgatcgg cgaggcggcc gcgtttgtgc tgctggaacg cggcgatgcc gcggcaccag
     7441 gcaacgtgct gctgaccgga gtgggcgagt cgagcgacgc atatcacatg tcctcgccac
     7501 atccggaagg gctgggcgcg gtagaggcga tgcgtgccgc gctgcgcatg gccggactgc
     7561 aacccgaaga cattgactac atcaacctgc acggtacggc cacacccagc aacgatgcag
     7621 ccgaaaatcg cgcggtcatg agcgtttttg gcgcagccac gccgtgcagt tcgaccaagg
     7681 gccatactgg gcatacgttg ggtgcggccg gtgcagtgga ggcggtcatc agcgcgctgg
     7741 cgctgggcaa tgaactggcg ccggccgggg tgggcagtac gcggctcgat ccgcaattcg
     7801 gtgtacatta cctgtttgac aaccgtagcc agccgttgcg gcacgtgctg agcaattcct
     7861 tcggcttcgg tggcagcaat tgcagcctgg tgttttccgg agcggcgcca tgaacggagt
     7921 gcggctgtat gtggacggca tcggcctgca tgcgccaggc atggcaggct ggtcgcaggg
     7981 gtgcgaggtg ctcggcgggc gcgctgcgtt tgtcgccgaa gtgccgccgc tggccgcacc
     8041 acaggcgttg ccgccgaccg aacggcggcg ggtcgggctg ccggtgcgcc tgagcatggc
     8101 gatcggcctg gaagcggcgc ggcaggcgca ggtcgatgtg gctacgctag caacggtgtt
     8161 ttcttccaca ggtggggact gcgacaactg ccaccatttg ctggacacgc ttgccagccc
     8221 ggaccgtacc gtatctccga cccgattcca caattcggtg cacaacgcgc cttcaggcta
     8281 ctggggcatc gctaccgggt gccgcctgcc ctcgaccacg ctgtgtgcct acgatgcgac
     8341 cttctgcgcc ggactgaccg aagcggtgag ccaggtcagc cagaccggcg aagcgtgttt
     8401 gctgctggcc tatgataccg cttaccctga gccgctgtac gcactgcgcc cgattccgca
     8461 tgcggccggg attgcgctgg tattggcacc gaagcaaagt gagcgctcgc tggcgaggct
     8521 ggagatcgta cccgatgcag ggctgcccgg gcgtatggaa gatgctgccc ttgagcagtt
     8581 gcgtacccgc gttccggcgg cacgcgggtt accactgttg caggcgctgg cgcggcagca
     8641 gccggctgaa gtcgtactcg aatatctgga tgcttttcac ctgcgtatcg gggtgatgcc
     8701 atgcatatga cgcgcgactg gctgtgtgcc catattccgc acgcgggagc catgtgtctg
     8761 ctggctgggg tcgaatcgtg ggacgcgcag cgcatcgtgt gccgggcgga cagtcaccgt
     8821 ctggcggatc atccgttacg ttcgggtggc cgtctgagca tggtgcacgg agtggaatat
     8881 gccgcgcagg ccatggctgt gcatagcgcc ttgctggcgc acagcgccgg aaagccggcc
     8941 atcggctatc tggccagtgt gcgcgacaca cactggtttc gggcgcggct cgatgacgtg
     9001 caggcggacg tgtgggttca ggccgagcgc atttcgggcg atgagcggct ggtgttgtac
     9061 cggttcgagc tgacggccga gcgcgagcgc ttgcttacag gacgtgcgag tgtgctgatc
     9121 gatgccgggc gtgtcggcac gccaatgggt gccggagggg caagctgatg cgtcgggcgc
     9181 tgatcaccgg gggcagtggc acgattggtg cggcgatcgc acgacgtctg gcggcggacg
     9241 gattgcatgt gattgtgcat gcgaatcgtg ccatcgacga ggcgcgccag ttgtgcgcat
     9301 cgctggttgc gtcaggcgct tcggccgaag cgctgcagtt cgatgtgacc gatgccgcgg
     9361 acacgcatgc ggcgctggca gcggtgctgg agcaaggccc ggtgcaggtg ttggtcaaca
     9421 atgccggcat tcatgacgat gcggtgtttc cgggcatgca ggccgagcag tggcatcgtg
     9481 tgattgacgt gtcgctgcac ggattctttc acgtcaccca gccgctgacc ctgcccatga
     9541 tccgcacgcg gtgggggcgc atcctcacga tttcgtccgt ggcaggcatc actggcaacc
     9601 gtggccagac caattacgcg gcagcgaaag gtgcgctgca tgctgcaagc aaatcgctgg
     9661 cgctcgaact ggcgagccgt ggcattaccg tcaatgttgt ggcgccgggg attatcgatt
     9721 ctgccatgag ccatgctgcg ttcgatgccg aagcaatcgg gcggctggtg ccgatgaaac
     9781 gcagcggtac gccggatgaa gtggcggcgc tggtggcttt tctggcctcg gatcaggcgg
     9841 gttatatcac cggtcaggtg atttcggtca atgggggcat ggtttagaaa gactttgcgc
     9901 catttcgcca gacccccact gtgcaacgtg tcgaaccgct catggcgaca gccgggtgcc
     9961 gcatgcgggc gcagattgtc agacgccgct tcgggtgcct ccgggcaatt caaggcggaa
    10021 gccccccagc gcaaaccagc gggcaaaaag atgaaaatcc cgttgctgtc ctggtgtcag
    10081 ttcgttaaac agctgctgcg cccgttgccg gtcgtgtgca tctgcgacat gccgctgtgc
    10141 gcttctgaac acgatcatga acggggccag aggcgtacag tggcggatga gcggcgtgcc
    10201 ggtcagccgc acggctgcgc tcagttctgc catgctgaaa gctgcgcgta gcgaattgaa
    10261 gtaatcgagg gtgaattcgg cggtttgttc cttgcgtcgg tcttcggcaa agaatcgctg
    10321 ggtgcgcggg tatttgaaac ggccgaaatc catcaggtac aacgcgccat cgtttttcag
    10381 tacacggcgc acggacagca tcgtgtgctg cagcgcagcc agatcgggca ggtggtggag
    10441 tgacagggtg cacaggacgg cgtccatcga ggcatcggca actcctgaca ggcaggtcat
    10501 gtcgtctgtc gccagcgtga cgttgtcgca tccggcgcgt cgcagggtcg cctgtgcccg
    10561 gtccagcatg gccggcgagg cgtccagacc ggtaaaacgg gtgtccggtt tcagccgcgc
    10621 cagtcgttcg agcaggttgc ccggtccgca agccaggtcg agcacgtggt cgcctggccg
    10681 caacagcggc gcgatctgca gcgcgtggta gacgtactgc gtcgccagaa tgcccggtcc
    10741 gcgtccggcg gtgtcgaatg cggcattctg gctggcatcg cgcatcacct gtgccgtttc
    10801 cggaatacgc acgcaggcac ggccgggcag gtgttcgagc agcaggtgtg cgagcatggt
    10861 cagccccgac attactgctc ctgtgctcgg tccattccgg gtgcaacctg tgggtgtttc
    10921 atgggcgttc acggcgaaaa ttgccacggc gggcgcgcag caggtgcggc aggcgcagca
    10981 caaaaccggc catcaggcgc agatgcatcc agctcagcaa ggcgttgtcg cgcaggtagt
    11041 tgaaatgcga tacccccccc tctccggcgc tgaagtaacg caccggtgcc gcaatgttga
    11101 tcggcgccac gccctgccag cacaggcgca ccacggcttc ggggtcgaaa tcgaaacggc
    11161 gcatccagcg ctggccacgc atgacgcgac gcagcggttc gatcgggtag acgcggaaac
    11221 cgtacagtga gtcgccgatt ccggcatgca gggtttccag gttagcccac cagttggaca
    11281 gtttgcgtcc ctggacgcgc agtgccggtg cgctggagtc gaagaccggg cagccgagga
    11341 tcatggcatc gggagactgt tgcgaacggt gcatgaaggt cgggatcagc gccgccggat
    11401 gctggccgtc ggaatccagg gtcagtgcat gggtgtaacc ctgacgcgcg gcttcgtcga
    11461 ggccatgcag cacggctgcg cccttgccct ggttatgtgg cagaacgagc acacgcaggc
    11521 cgctgttttc gtgggccatg gcacgcaagc cgtcagcgct gccgtcggtg ctgccgtcca
    11581 cgattaccca gaccggattc cattgtgcga gggcttcgcg tacggtcgca tagacttttt
    11641 cgccgggatt gtagctgggg atcagcacca ggtgcgtgcg cgaaggagaa gtgatgttgg
    11701 gattcatggc gaaacggctt ccgcggtgac gcgcaattcc tgttcaaaat aggcatgcag
    11761 gttgcgggtg aattcacgcg gatcggtcgg ggcatcaaaa cgccgcccca ggcgaatgcg
    11821 gaaatgcatc ggcatgtccg ggcatgcaaa cagcggttga cccttgccga ggaagcgggt
    11881 gtcctgttcg atgatcaggg tctgcaccgg catgccggaa cggtgggcca tgatcgcggc
    11941 tccggcaaga aaacggtcaa gcggcgggtg gctggtgcga gaaccctccg gaaacagcag
    12001 caactgactg ccctggccta attcgtcaat cgcctgatgc accgagccga ggaaacggtc
    12061 attacggatg tagcatgcca gacgcgcgcc cgcaccccag aatacgttgt cgagtaacga
    12121 tgctttcagg atgcagacga cattcggcag gcgcgacagc accatgggtg catcgagcag
    12181 accggggtgg ttggcagcga gaatcagcgg gccggcgtcg cgcagttcat ccagcgccgt
    12241 caggtcgaag cgcgcggccc ccatgaggtg cagtgcgaac agatatgtgc ggaatccaag
    12301 catggccacg cggcggccga agcgggcagc gcgacagcag cggaacagtg gcaataatgg
    12361 caaagcaatg gccgcccagc tgagacaacc gatgccaagc agggtgaagc cgccgaacag
    12421 gatgagttgt tcgcgcagat gtcgccatcg tcgcagtggt cttgcagcgt gtggggttcg
    12481 cgccccgtca gtggtgtgcg ggcggtgacg tttcgcgcta tccagcggca ggcgcaggtt
    12541 catgggggcg taccctgaac cgggcgggtg gcgaaggctt cgatttctac cagcagttcg
    12601 ctgcgacaca catctgcttc gagccaggtg acgtcggcat cctggccgag cagaccatgc
    12661 acggtggcgg cgatgccatc ccggtcgctg ttgtgacgga cgtaagcctt gaattgcaat
    12721 gcctggctgg aaaaaccgcc accgcgcgcc agccggttgg cctgttcgac cagtgcgtcg
    12781 atgttgtgca gcgtttctgc ggtctgcagg gatgcattgc ctggatgccg gctttcgtgg
    12841 ccaacgatac tggcggtgcc agaaatgaac aaagcgtcga ggtcgtcgaa tgggagcagc
    12901 agggcgcgcg agaaggtcgg gctgcagggg ccgtagcgcc tgggatagcg gaacgcgctg
    12961 atctggcgcg gattctcgat tggcagcggc tggatgcgtg tggcgataaa atacagaccc
    13021 agcgttccgc tgcgcgtgcc gagggcgctg gcggccggcg ccccgtccag gtaggcctgt
    13081 ccggcctggc ggaaggcgcg ttgacggcca atgttgaact gctggtatcg ttccagaccc
    13141 tcttccatgg cgtgaatatg aggtatgtag ttccagcagc gtgccagcca ggggaagccg
    13201 gtggcggtca tgagcccgaa gatggcgcaa taggcatcga agctcagctg ctccaggccg
    13261 acgctgccag cgtcgtttgt cgtggactcg ggcctcaggt agccgaacag caggtcgtcg
    13321 ttgtgccggt agtgcagcgg tccgtgtgta ccggagctga ccgggccccg acccagccac
    13381 gcggtccgcg ccaccgggaa gttcgccagc actggtgtgg cgacgaggca gtctgccgtt
    13441 gaatcaccgt acagtccata gtctaccgca cccagcaggc gctgcgtctc gggcgtatcg
    13501 ttcgcagcga gtttcagcca gtgcagcgaa tgctggtcag aaagcatggt gtctgcggaa
    13561 tacgagcacg gcattgctgc cgccgaaggc gaacgaactg ctcatggcag catcgaagcg
    13621 ctggtcgccg atgccttccc cggtgacgtg gaggacgccg gtgcaggctg gggcgatatc
    13681 gtcgaggtga gcggttggcg ggatttcctg acgagaaagc gccagtacag tcactaacgc
    13741 ttcgatggcg ccggcagctc cgagcaggtg gccgtgcatc gatttgctgg cgctcacggg
    13801 cagtcgccgg gcatgttcgc cgaagacggt cttcagcgcg ctgatctcca cggggtcgcc
    13861 ttcctgggtg gcggtgccat gcgcattgac gtagccaatt tcttccgggt gcagactggc
    13921 gtcgtgcaag gcgttgcgga tggcgcgtgc ctgtccgttc gcgtcgggcc ggacgatgtg
    13981 ggtgtggtcg cagctctggc catagccggc cagctcgcag tagatgcggg cgccgcggcg
    14041 cacggcatgt tcgcgttctt ccagcaccag cgcagcacct ccttcaccga gcacgaatcc
    14101 ggaacgggcg ctgtgaaatg gccggcaggc gcagccggct tcgtcctcgt tcgctgccgg
    14161 ggcgatcaca cgcatggctt cccaggcgcg catcatggcg ggtgacagcg gagcttccga
    14221 tccgccggcc accatgatgg gcgcctcgcc cgagcggatg cggcggaagg cttcgccgat
    14281 ggaaattgcg gacgaggcgc aggcaacgga atgcgtcagg caggcaccac cgaggccgag
    14341 tgcaatggcg atgtgcgctg ccgcggcatt gttcatggtg aacaccaccg aaagcggtga
    14401 cggccgcggt ttgccgcgct cgtagaaatc gctgtaaccc ttgtcgacgg tcagtacgcc
    14461 gccaccgccg gtgccccatg acacgccggc attgtcaggc atggattgtc cgcgctcgaa
    14521 accggcgtcc tgccaggctg aaaaggcagc ggcaataccg agttggctgt agcggtcagt
    14581 catgctggaa atggccttgc cgagcatggt ttcgggtatg aacccgcggc attgtaccgt
    14641 tggctgggta aatggctcgt gcggcggttg cagggagaag cgacttagcc tggttgccga
    14701 atcgccgcgc atgaggtggc tgaagaacgg tgtgtcgtcg ccgaaaggat caatcaggcc
    14761 cacgccggtc acgaccaccc tgtgctgtcc gctgaaactc atacggttcc gtttttggcg
    14821 gcgggttggg tgctttcgat gaaacggatc agttcgccga tggtttccgg tcgggcagta
    14881 tcgtctgtga agcggactcc gtaagagtct tccagttcaa agagcagttc gtacatggcc
    14941 agagagtcga tgcccagcgt atcgagacgt gtttcctcgg tcaggctggg gggcggattg
    15001 tcagtgtgac gttcaaggta atcttggatg agttccagcg gttccatggc ggatggcgga
    15061 tggtaaatac ctacggatta tagcagatct ggcatgcctt gtagcccctg tggctgttga
    15121 ttgaagagga aaaagggatt tgtcacaaaa atttaatcgc attcctgagt gaatcctaag
    15181 gctgtcttca cgggacagaa aagttgctgc cagagaatgc caactttggt tgcggatgcc
    15241 gcaaattgcg tacgaggtgc cggcgtcggc tgattttatc caggcagcaa acaggggaaa
    15301 cgcatgaaaa aggaagtggg ttttgtgttg aagccattgc cggcgctgct ggcagggctg
    15361 ttgttgggtg caccgctggc gcatgcagac ggggcggtcg atcttggcag cgtatcggca
    15421 cagggaacga gcagcaacgg cacctcgaac aatgccgaca cgaccaattc gagcacggtc
    15481 tggaacacgc agcagaagca gaattcatcg caggatgtgc agtcgatcac gcccgatcag
    15541 atccagctgt tcggaccgga tgcgggcggc atgcaatcgc tgtcgatcat gcccaacaca
    15601 ctgatcagtg gttacaacgc gggcagtgta tcgtcgcgct ccaccatttc catgcgcggc
    15661 atcaaggtgg gctggaacag cgtgccgggt gacctgcaga ccaacggcat caccgccgag
    15721 ctggatggcg tgccgctcaa cagcctgagt cagggaacgg gctggcattc gccggaaatc
    15781 ccgatcggtg cgctgatggc gggcgtgaat accatcaatg gtccgggaaa tgccgatacg
    15841 cgctggtaca acacactggg cgggtcgatc aatttcattc cggtgcaacc cagtgcccag
    15901 gcgagcgcca atgccgagct gtcggtcggc agtttcgata ccctggtggc atccttcgtg
    15961 ctcaacaccg gcgagcatga cggctggtcc accgtgcttg gtgccgccaa cgcgcgcagc
    16021 cagacgtttc gcaccaccag cgactacctg ccatcgcgca ccaccgatgc ctacatcaag
    16081 acgcagaagc gtctcgacaa tggcctgatc agcttcggtc tgtatgggct gtacaacgat
    16141 gagtaccggc cgaacatgat ccccacgacg gcgcagccgg ggatcaccat gaacggcatc
    16201 ggtatccctg gcccggcgta tagccagcag accgcaggct attatgcgac gttgccgacc
    16261 gacatgtggt tcaagcacaa ttcgatcgaa gattaccttg gctggtcgca cctggaactg
    16321 aacctggcac ccgatttcac cctgagcaac gtgatgtggt tccgcgacgg ttatgtcaac
    16381 cacttccgca tgaataacta cgattatgcc ggggtgggta ccaacgaaga gaatttcaat
    16441 gagcgctcga acacctatgg cgacaagctg cagttcgatg agcacttcag caagagcaat
    16501 gtgttcagct tcggcggtta tgtcatcagc tcgcgtgctg aaagtgccta taccggttat
    16561 ctgtccgcgc tgggtttcaa caacagtcag ccgggttcga tccagtacaa caccacgcag
    16621 accactgact gggcactgtt tgcccaggac agcgccacgc tgtttgaccg gctgaacatc
    16681 gtaccggcgt tccgtctggt ggatttccag accgatttcg ccaacaacag cccgaatgcc
    16741 gcgagcacct attatccagg tggtatcccc ggcagtgtct cgtacgacac caatccgaac
    16801 cagtcgacca cgttcgtcaa gggtgagcca tcgctgggcc tgaactacaa gatcgacgat
    16861 gcgctgaatt tctatggcaa ctatgccatt gcctacagca acccgactgc cggcaactat
    16921 gaccgttatc cggaaaacat cgccacgctt cagccgattc gcagcaccaa ctgggatatc
    16981 ggtctgcgct atgcgcagaa aaacgtgttc gggcttgacc agttgagtgg caccatcggc
    17041 tatttcaata ccctgctcga taaccagacc atcccgcgca ctgtaaccgg ttccatcgtt
    17101 accacctttg gtagcggttc ggccacgctg gaaggcgaag aactggagat caatgccgac
    17161 atcaaccgca actggtcggc tttcgccaat cttggctggc tcactgctac ctggaattcg
    17221 tactattcgg gtaccaccgg tcagtactac aacggcatgc cggtgtccaa ctcgcccgag
    17281 cagaccacca acgtcggcgt gacctacgag gcctatgtgc ctggcggtac aatcgacacc
    17341 acgttgtggg accagtactt cggcaaaagc tacatgtatg acaacctgac tggtgcacca
    17401 tccagccagg tcaacccggc gtacaatctg gtcaatctgt cgatcaacgc tcacctgacc
    17461 tcgatcggct ggctgcagtc gctgctgcat ccgcaggtcg ccacgctgtc gctggatgtg
    17521 cagaacctgc tcgatacgca atacaattcg acggcctaca tctctgctgg cggttatttt
    17581 ggcaccagtt acggcggata tgtcattgcc aacccgggtg cgccacgcag cttttttgtg
    17641 actttgcggc tggattactg agtcacggtt catgtatcgc cgacagggcg gggggaaacc
    17701 cccgccctgt ttgtttctgg tcagctggaa ttcattctgt tctaagtgtt ttttttatat
    17761 aatcgtccca tgttgacgaa ttccatggca atcaggctgg gacgcggcgg cgcgggactg
    17821 gcgctggtgt tcatgctggc cgcatgtgcg agttatgctc cccggccttt gccggaccgg
    17881 ttttctgccc cgaccgctgc cgggcaggtg caggtggatg ccatgcgcct gccgccctgg
    17941 gcggtacatg tctggaatcc ggccgatggt ctcgacagtg atgaactggc catgctggcg
    18001 gtggccaata atccgcagct gcgtgcgaca cgcgcggccc tgggcatcgc acacgcccag
    18061 gtatttgctg cggggctgct ggctgatccg cagatcggtt acagcgaaga cctggccatg
    18121 acgcccggta cgcaaaatgc gtgggcattg ggacttgggt tcgacatcag cgactggctg
    18181 atgcgatcat cgcgcaagcg catcgcgcag gccggcgcaa atcaggcgga acaggaactg
    18241 ttatggcagg aatggcaggt ggtcaatgcg gccagactgt cctatgtgcg catgcgtgcc
    18301 gacgaacggc tgctgccgct gctgcaacag ttgcgggaca gcctgcgccg gcgttatgaa
    18361 gccagccgcc gggcgggaga tgccggtaac ctgactgccg atgccgtggc agcggactgg
    18421 ctgacctggc agggcgcgca gggccagctt gatgatgcgt atcggttcga acggcaggat
    18481 cgggcggtca tgaatgacct gaccggactc gatgcagcag cccgagtgcg tctgagcggg
    18541 gatttcgttg caccgaggct ggatgtggca gggctggtgc ggcagttcga tgcgaggttg
    18601 cgcgagcgcc cggacatggc ggcattgcgt gccggttatg atgcgcagga ggccggctac
    18661 cgtgcggccg tactggcgca gtttcctgtg ctgggtgtga gcatcaatcg gtcgcaggac
    18721 aactatggcg ataacagtgc cggtccgggc gtggtgctga gcctgccggt gttcaaccgc
    18781 aatcgcggca atattgccgt ggcgcgggcc agtcgtcgtc agctgtatga agaataccgc
    18841 gctcgctggc tggctgcgca aagcgagttt cacgtggttc tggatatctt gccccggctt
    18901 gaggctcagg ttgccgaagt tcgccggggc atgccggcgc tcgaggctca ggcgcacatg
    18961 gccgagcgtg ccgaacacga aggcaatctc gcggcgcccg atgcggcgcg catgcggctc
    19021 gcctggattg ccaagcacat cgaactcatt catctcgaac aatcgttggc cgaacagcgc
    19081 attgcgctgc agaccctgtt ggggccagcc cttccggagc acccgatacc atgatgtttc
    19141 acgttccttt tgcgctgcgc tggctgctgc cgtgcctggt actggtgttg ggcatacagt
    19201 ggtcggcact ggccgatgac gatgattccg cagtcgttcg gccgacattg ccgccggtgg
    19261 ccagcgtgca tgtggtgccg ctgcgcatgc agtcggtttc gcagaccgtg gtggcttatg
    19321 gtcaggtggc gcaggatccg gcacggctgc agacggtcag tctggcgcgt gcgggtcagg
    19381 tgatccggct ggcggtgcgc gcaggtcagc atgtgcgtcg gggggatctg ctgctgacct
    19441 ttgcccgcag cgcagccgtg acgcagacat ggcgtcaggc gcagacagcc gtgcagtatg
    19501 ccgaggccga gcgcgcccgc gtggcgcagt tgcgctcgca gcaactggca acagtatcgc
    19561 agctggatgc cgccgatcgg gcgctggccg atgcgcgatc ggcgctggag gctgcgcgca
    19621 gcagcggttc cgacagtgca cagaccgggg tgcgcgcgcc gttcgacggc tgggtacaat
    19681 cggtgtcggt ggcagtcggc gatcgggtgg cggccgatgc gcccttgctg cagctggggc
    19741 cggagtcggg gcagggcgtg ttgctcgatc tggatgcggt tgatcgccag cgcgtgcatg
    19801 ccgggctggc ggtgcgcatc gtgccgctca tgaccggttc gccagcacag gatggcacgc
    19861 ttgcatacct ggccgatgtc attaacccca cgacgcagca ggtgagcgcg gtggtcaggg
    19921 tggccggaca cgggctgctg ccgggcgagc gtgtgcgtgg cgagatcgcg ctcggtgcgc
    19981 acagggcgct ggtggtcccg cgtacggccg tgctg
//
LOCUS       DAIANL010000093        10016 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_933962, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000093 DAIANL010000000
VERSION     DAIANL010000093.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10016)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10016
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933962"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2136 a         2694 c         3245 g         1941 t
ORIGIN      
        1 aaaataaata ttaaataata acttaacgca ataattaaaa taattaactt ttgtttatta
       61 aatgtctctt gcacaatagg ttcaaccgtc gaaacccaaa tgtggcggtc ccgacaaaca
      121 ttctggcaat agcactagga gagcaagcat ggcagcaaaa aagtatgagg ctggtgtcaa
      181 ggagtatcga cacacgtatt gggcgcccga atatgttccg ctggattcgg acatcctcgc
      241 ctgtttcaag atcaccccgc aagcgggtgt ggatcgtgag gaagccgctg ccgccgtagc
      301 cgctgagtct tccaccggaa cctggaccac ggtgtggaca gatctgctga ccgatatgga
      361 ttattacaaa ggccgtgcct atcgcattga ggacgtgcct ggtgatgaca cctgctttta
      421 tgcctttgtg gcctacccga ttgatctgtt cgaggaaggt tcggtggtca acgtctttac
      481 ctcgctggtc ggcaacgtgt ttggtttcaa ggccattcgt gcattgcgcc tcgaagacgt
      541 gcgcttcccg ctcgcctaca tcaaaacctg caatggtcct ccccatggta tccaggtcga
      601 gcgcgacaag atgaacaagt atggccgtcc gctgctgggc tgtaccatca agccgaagct
      661 tgggctgtct gccaagaact acggtcgtgc cgtgtacgaa tgtctgcgtg gcgggctgga
      721 tttcaccaag gatgacgaga acgtcaactc gcaacccttc atgcgctggc gcgaccgttt
      781 cctgttcgtt caggatgcca tcgaaaatgc ccaggctgaa acgggcgagc gcaaaggcca
      841 ttacctgaat gtgacggccc cgtctccaga agaaatgtac gagcgtgccg aatttgccaa
      901 ggagatcggc tcgcccatca tcatgcatga cttccttacc ggcggcttta ctgccaatac
      961 gggtctggcc cgctggtgcc gcaaaaacgg tgtgctgctg cacatccacc gcgccatgca
     1021 cgccgtactc gatcgcaatc cgcaccatgg catccacttc cgtgttctgg ccaaggcgct
     1081 gcgcctgtct ggcggcgacc acctgcactc aggcacggtg gtgggcaagc ttgaaggaga
     1141 tcgcggcgca accctgggat gggtcgatct gatgcgtgaa tccttcacgc cggaagaccg
     1201 ctcgcgcggc atcatgttcg atcaggactg gggctccatg cccggtgtga tgccggtagc
     1261 ttccggtggt atccacgtct ggcacatgcc cgcactggtc aacattttcg gagacgattc
     1321 ggtgctgcaa tttggtggcg gtactttggg acacccatgg ggcaacgccg ccggcgctgc
     1381 cgccaaccgc gttgcactcg aagcttgcgt cgaagcgcgc aatgaggggc gtgagctgga
     1441 acgcgaaggc aaagacattc tcaccactgc ggccaaggac agtccggaac tcaagattgc
     1501 catggaaacc tggaaagaaa tcaagttcga attcgacacc gtcgacaaac tggatgtggt
     1561 gaaccgttaa gctgcggccc atattcggga cgtaatcgaa aacctgcgca gcgcgtggcc
     1621 gggtggcccg tcaagcgcta caagcaaagg aaagaaaatg gctgatattc aggattacaa
     1681 ctcgacaccc aaatacgaaa ccttttcgta tttgccggcc atgacgccgg aaaaaatacg
     1741 tcgccagatc aactacatcg tgagccaggg ctggaatccc gggatcgagc atgtggagcc
     1801 gtctcgcgcc ttcaattact actggtacat gtggaagctg cccatgttcg gcgagcaatc
     1861 cgtggaaacg atcattgcgg agctggaggg atgtcatcgt gcgcaccccg gccaccatgt
     1921 gcgtttgatc ggctatgaca actattccca aagtcagggc agttccttcg tggtattccg
     1981 cggccggtaa tccggtatcg ggcgagccgg tcggggttcg cccagggaaa cgatagcaat
     2041 tggaaggatt aaagcatgag cgacgctatg gcagaaatca gaggagcagc gccaaaaggc
     2101 gcgaatggac gtatggtctc gcgtgcacgg cgtcaggcac tgtcgcacag cggcaagtcg
     2161 ggtctggcgc aggttggcaa ggccgcccct gcgcccgcgc cagccaccgc gccggcgtct
     2221 gatgtggcgc atgctgcggc tccggagcca tccagagacc tcggacgatc catgggaaac
     2281 ggcaatgaaa cacgtgcctg tggttgcgag catgctgccg cgcaacccag cctggaagcc
     2341 gtgctggacg aggtctgcga actggcggaa gcggtgcccc atgctgttgg aatgggtgct
     2401 tccggtgtgc gtcaattgtg ccaggaacga cgccgggcgc tttcgagcca aggcaaaacg
     2461 gctgtgaaat ccgccgccat gagcgcatcg aagcgtcctg gcatcttcaa gggggtggcc
     2521 gctgccggtt tgagtgggcg tgccgccgcg cgggcgcgcc gtgaagagat gtgtgtcaat
     2581 ggtcgcggta gtgacccggc atgccgacct tccggccgtg tgcgcactca ggcgaatccg
     2641 gccaaggttg aggtcggtac gaccttgagc ggcaataccg taagtggcac ccaggttgaa
     2701 cgtacccgca aggtcaccgg agtggaaacc ggtagctgcc gcgccattac cggtactgaa
     2761 tacatcggta gcgaacagta cacgggcttt tgttcatcga cacctgatgc cgcggcgccc
     2821 aaggtcagct ttggcaccac cagtcgtggc cagctcgtca gcggaacgga agtcgggcgt
     2881 agccagaagg tgaccgggga tgaacacggg gagtgtaggt cggtgactgg cacggaatat
     2941 ctcggtgttg agcaattcga atcgctttgt gctgcaaagc cggagccggc gccagcgaaa
     3001 gtgggtgtgg cggaaacgcg cgccgggcaa agcgtaagtg gaagtcagat cggacgcagc
     3061 aaccgggtga cgggtgatga gcctggtgcc tgcctcaagc tgaccggcag tcagtatgtg
     3121 caagagcagc ccggcagtgc gtgtcgtacc ggcagcggca agccgcacaa ggtgagcgtc
     3181 atgagcaccc tgaaggatcg tccggtgacc ggcaccgacg ttgcacccgg aagcatggtt
     3241 accggcggcg agcgcggcgc gtgtgccgat gtaaccggta cggaatccag cggccttgag
     3301 cagtaccagg gatgcaaccg caagccggta ccggcaccgg aaaaggttgg cgtgatgcgt
     3361 acctggcatg accagcaggt atccggtact gcgatggaac gcagttccag ggtgaccgga
     3421 gatgaatatg gcggttgcca gccgatctca ggaacggaat acatcggccc ggaacagtac
     3481 acgacatttt gcgacacgga tcgtcaggca gcctcgcgag cgctgatggc aagccgtggt
     3541 gcacgggctg gcgtggcgcc aagcggaaac cgtgtcgaat ccggtcacaa ggttaccggc
     3601 gcagagcgcg gtgaaaacca gatgctgagt ggaacacctt attcgggtcc ttttcagcgg
     3661 atgtcgcagc gcgacgccaa cagcaacatg catccgatca accgtggtcc agccagtgcc
     3721 gtgcgcgagc aagaatacgc accgcgggag ccggaaatgg caggcagttt cagcattgtc
     3781 accccggcac gtggtgcgcg cgacaagatc atgaatcgta tcaccggcac cgcctatggc
     3841 gcagtcggcc gcattaccgg gccgatcaac atggcagccg gcctggtgtc gggtacgcct
     3901 gagtttcgct atcaggagga catgccggcc ggggttgccg tgcaggtttc ggcagctgcc
     3961 gccgtgcgca gccgtttgac cggtgatggc cgtgaaggcg ggattgcgat taccggcgcg
     4021 gcctggcgac gcaatgagta tgtcaccgga acagaaagca cctcgatgct gcgcaatccg
     4081 acgttgcgca gtgaacggcg cggcgcaccg gctaccgcaa ttggcgcatc gcagttgaaa
     4141 gaccgcgagc gggcagaggt ggcagtcagc aaaattaccg gcagcagtgg taatgatgcc
     4201 aagggttcgt acatcaccta ttccggcggt gcgcgcggtt gatatgacgc aggcgatgct
     4261 tgaacaaact caaacggatc gaaaagcgaa atgaatacga gaaaccgccc gggacgcaac
     4321 attcgcccgc aacctgcagt gaaccagccg ccttacggat tgggtacggc tggcggtcag
     4381 ctgcaggggc ggcaacctga accagctgcg gccgcggcgg tggtgccaaa tccggcgtgc
     4441 acgatccagg gtgggcgtct gtgtcatcac cccttgacga acgagatgga gaaccagcgt
     4501 ctgttcaggc acgagaaatc cgtcagggaa cgctttgcca gcatcgttcc ggtgttgaag
     4561 ttcattgccg gcttgcagca cgaacccgat ttctgcgagc gcgcgcaatc gatcgcaatc
     4621 aaccagttgg ggtttgagtt gccggagcag gttctggcgg atgcctgggt ctccggcctg
     4681 gatatgcggg cgttgtatgg tgagtgtgtc atgcgcatgc agtacttgct ggcacagcag
     4741 actcgcgagg caggtcagcc gtcggggaat gtggatcagt gcatggacga ggcaatgacc
     4801 ttcttcctgg actgcaacta ccatgcggtc gatatttcgg catgttcgga cggccgtttg
     4861 aaagggctgg tgcgctatat cctgcgtttg cctgacggtg cggtgcgcag caagaagtcc
     4921 tatgccgggg ccatgttcga tgtggatgcc aacgtcaagc gctggatcga aaccgaactg
     4981 atgcgctttc gtgaaggccg tccggtgacg gccgatgcag gaacccgtta cctcaaggtt
     5041 tcggtgtatc actggagcag ttctgacccg ggccacgaag gatgtgctgc ccatgcgagc
     5101 agccagagcc aggcggcaga acaagccttg aaacgtttgc gggaattccg tcaggccatt
     5161 gaaaacagtt tttgctgcgg agcctcggta gatacgctgt tgatcggggt ggataccgac
     5221 accgatgcca tccgggttca cataccggat gcggatgggg aaatgagcga gtttcgcgtg
     5281 gtggacaaca tggaagtgta tcgccagacc gtcgaggacg acaagaacgg cgcacacctc
     5341 aaggtgtatc aggcgatccg cgctgccagt caggcaaccg gttggggtgc gggcaatggc
     5401 gagccgcatg aaggcatgcg ccgtctcatc gccacgctgc tgatcaacaa cttgtcgcag
     5461 atcgagtatg tgtgcagtaa ctggggcggg cggtatgagg acatcggtca cgccgaacgt
     5521 ttcatcaacg tcggcgacgg actggaagaa ttccagttgc gtaacctggt ttattttgcg
     5581 cacttgcata cggtggaaga aggcgcgccg gatctcgatg tcgggatcaa tatcttccgc
     5641 aagctcaatg tggcgcatgg cttgccggta ccggtggtaa tccacttccg ttacgacagc
     5701 cgcgtgcctg gctgtcgcga gcgcaagatg gaaagttgcc ggcgcgtcag gcgggccatc
     5761 gagtcgcgct acgctgatct cgcgcaaaaa ggattgctgg tgtgcggcat gatggtacag
     5821 gacaaacagc ctggaagccc gcttgaaagg gttgaaacgg gtgcggcata aattggttcg
     5881 gggatgagcg atgaaaatct gtcaggtgga aaagactttg gtttcgacca accgcatcaa
     5941 ggaattcgga caccgtccct tgcttgtggt gcgggaaaaa ctgggcggcc ctcgtcaggt
     6001 ggcggtggat gccgtgggct gcatgccggg cgactgggtc atctgcgtgg gttcctccgc
     6061 cgcgcgtgat gcggctggcg ccaaggactt cccgagtgac ctcaccattg tcggcatcat
     6121 tgaccactgg tcggaagcct gacatggaga tcatgcgcgt agtgtctgac ctggtggcca
     6181 cgcggcgtgc gcctggcctg aaaaacgcat cgctgagggt ggttgaagac gcccagggga
     6241 agatgggggt ggcgtgcgat ccggttggca gccctccagg caagtgggtg attaccacca
     6301 gtggcagtgc agcgcgtatt gccaccgggg acaaagccac gctgaccgat ctgaccatct
     6361 gcggaatcat tgattcatgg gacgccggta acggcaaata agcaactgtt ttggttttta
     6421 aatacaagga gtagtgaaaa tggcaagcgt aacaagcgta accggggtgg cattgggcat
     6481 gatcgaaaca cgcggcctgg tacccgcgat tgaagctgcg gacgcgatga ccaaagcagc
     6541 tgaggtgcgt ctggtgggtc gccagtttgt gggtggcggc tatgtgaccg tgctggtgcg
     6601 tggcgaaacg ggtgcggtca acgcggccgt gcgtgccggt gcggatgcgt gcgagcgtgt
     6661 cggtgacggc ctggtcgcgg cacacatcat cgcccgcgtg cactccgagg ttgagcatat
     6721 tcttcccgga tccgtggcgt aatcaggcag caagcaattt tcaattaacc gaatcagcaa
     6781 acaaggagta atcaagatgg caaacgtaag cggaattgca ctgggcatga tcgaaacccg
     6841 tgggttggtc ccggccattg aagcggcaga tgcgatgacc aaggcggcgg aagtgcgcct
     6901 ggtgggtcgc cagttcgtcg gtggcggcta tgtgaccgtg ctggtgcgcg gcgaaaccgg
     6961 tgcggtcaat gcggccgtgc gtgccggtgc ggatgcgtgc gagcgcgtcg gcgacggcct
     7021 ggtggcagca cacatcatcg cgcgcgtgca ctctgaagtc gaaaatattt tgcccagcgc
     7081 ctcggagtga agcggccctg tcgttactga tacctgaaat ggagaaccaa aaatggcaaa
     7141 tgtaagtgga attgcactgg gcatgatcga aacccgtgga ctggtgccgg ccattgaagc
     7201 ggcagatgcg atgaccaaag ccgcggaagt gcgtctggtc ggtcgccagt tcgtcggcgg
     7261 cggctatgtg actgtgctgg tgcgcggtga aaccggcgcg gtcaatgcgg cagtgcgtgc
     7321 cggtgccgac gcatgcgagc gcgtcggtga cggcctggtc gcggcacaca tcattgcccg
     7381 cgtgcactcc gaggtggaga gcgtgttgcc gaacaaggcc gatgcgggcg acagcggact
     7441 ggatggtgtg gtcagtcaat ccatgagcta gggcaggtgg ggttatggcg atcgatccga
     7501 gattgacagg gtatctggcg cgtgcgctca gccacgagat gttggcggtg caacagtacc
     7561 tgacccaggc cagcctggtc cggatgtggc agtttgaaga gcacagcaag cgtttccgtg
     7621 acgatgcgca ggatgaactc ggtcatgtgc agcaattgat cgaacgcatg ttggcaatgg
     7681 gcatcgcttc gaaagccacc cagctgcaac ctgtccggac tgggcgaaca ctggaagaaa
     7741 tactgttgat cgaccgggag ctggaaatcg acgtgatccg cctgtatgaa gaagcaaccc
     7801 attattgcgc caggatgcgg gatggcgaga cacacgcgtt gtttgccggc ctgctgcagg
     7861 atgaactcgg ccacctgcgc gatctggatc aaatgttgaa tggcatttac aggggaacga
     7921 catcatgagc ggtactggtg acttgaatgc agcgggtgtg tccgtgtcgc aggaacgcct
     7981 gcctgcgctg aacagacgtt tcgattttgg tgcttacgcc gagatgcggc attttctgga
     8041 tcagttggcg gacttgtccc gacgtgagga tttttacccc aatgtgagtt tcggtaaaac
     8101 ctacgccaac atcagtatcg aagccgcgga tcaggccagg ctggttgaat gcaactcccg
     8161 attcatcaac gagatggaag cgttggctgc atcggtaaag tctcaataat cgatctgcca
     8221 gagcgggtgg cgggagtcaa catggcggcc agggtcattg cggtaatcaa tcacaagggc
     8281 ggaaccggca agaccactct ggccctgaat ctggcggcag gtctcgcgcg acgcggaacc
     8341 acccacctgg tggatgcgga tccccaggtc tcgatcacgc aatggtgtgg tatgggcaag
     8401 gacagatccg ctttgccgga ggtggtgcca ctgcacggag atcccgcggc cgtgctgggc
     8461 agactgttgg aaagccaccg ctatgtggtg gtggattgcc caccgacggt acatggtgaa
     8521 gtggtcgcag tcatcatgcg catggcgcag gtggtgctga ttccggtgct gccatcgccg
     8581 atcgatttgt gggccagtgt ggacatggct gcagcgctga aagcggcgaa aagcggtaat
     8641 gccggcttgt cagcccatgt ggtgctcaat cagctggaat cgcgcaatgc gttgtcgcgt
     8701 gccatgcgcg aggccgtcgc ggagtttgat gtgcccgtgc tggctgccgc tatgcagcgg
     8761 cgcgcggctt atcgcagtgc tgcggtggaa ggtttgagtg tgtacggcct gggtaagcgc
     8821 ggcctgttag ccgtggcgga tatcgaagcc ataatcgagg aggtcttatg cctgtaaaaa
     8881 atacgcgatc aaagctggta caaggcgtac gccagatcaa ggcacagcag gacagtgcgc
     8941 agtccgccgt gacagccgaa gctgccaggg atgtgggatc agccgttacg acgccagccg
     9001 caggagacat tacgacgccc gccgcagcca ggccggaagc tggcagagtg cgcggcgcca
     9061 ctgtactgca tccggacagg gtgtggccag actgagtcag gtcatgcatg tgcgccagcc
     9121 tcgctggcgg atgcaggttg cctgcgtcgc aggacttccc ggcaagacag ccgattgatt
     9181 gctggtacgc acccaatgga ttgggctgat ggcggcgcga cccgtgtcgt gatgacctgc
     9241 cgcctgtgaa tactcccgaa aggaatcacc atgaacgatg tgatcaacca gtaccgtatt
     9301 gaaaaagaac cctattattt gcccatctcg gacgaagttg cgctgtatca ggcagcctac
     9361 agcgtgcgta tgccgatgat gctcaaaggc ccgacaggct gcggcaagac acgttttgtc
     9421 gaatacatgg cctggaagct cggcaagcca ctcatcaccg tggcctgtaa tgaggacatg
     9481 acggcttccg acctcgttgg ccgttttctg cttgacgcga atggcacgcg ctggcaggac
     9541 ggtccgctca ccattgccgc ccgttacggg gccatctgtt acctggatga agtggtggaa
     9601 gcccgccagg acaccaccgt ggtgattcac ccgctcaccg attcccgccg cgtgttgccg
     9661 ctggagaaga agggcgagct ggtgcatgcc catcccgact ttcagttggt gatttcctac
     9721 aaccccggct accagagcct gatgaaagat ctcaaacagt cgaccaagca gcgattcggc
     9781 ggtctggatt tcaattatcc cgagcatgcc attgaaaccg agatcatcgc tcatgaaacc
     9841 ggtatcagcg cgcaggcggc tggcaatctg gtgtccatcg ccgagcgtgc gcgcaatctc
     9901 aagggccacg ggctggatga aggcatttcg acgcgcatgc tgatatatgc gggcaatctg
     9961 attgccaccg gtgtcgatcc ggtcaaggcc tgccgggtga cgctggtgcg tccgat
//
LOCUS       DAIANL010000094         5166 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2366402, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000094 DAIANL010000000
VERSION     DAIANL010000094.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2366402"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          975 a         1478 c         1676 g         1037 t
ORIGIN      
        1 gctggcgctc gatccgcagg gggtgctgtt gctgccgttc aatccgacgg ccgagaacat
       61 ggcgcgctac ctgctcgaag agatcgggcc gcgtcagctg accggcctgc cggtacggct
      121 gacgcaggtg atcgtcgaag aaacggcgaa gtgccaggcc gacgcgctgc tgctgcctga
      181 cgactgaggc acggcgggcg cgcggagggg ggtgcgtcag gccacgtcca cgatcatctt
      241 gccgaaggga aagaacgata gctgcgccag cttgaggtgt tgccaggcga acgggagccc
      301 cacaatggtg atggcaagca gcgcagccca gaacaggtgc gccagcgcca gccaccagcc
      361 ggcgcaaagg gcccagatga ggttgccaag cgcggcgccc gcttcgccaa tcgcgccaga
      421 tggccggtcg acgatggcct tgccgaacgg ccagaaggtg aacgctccga tgcggaaggc
      481 cgcgatggcc cagggcagcc cgacgatggt cagcgccgcc accacagcgg cgaaccacca
      541 ggccagtccc gacaggcagc cgccgcagaa aaaccatacc aggttggcca acagacgcac
      601 ggtgaaactc ccttgattgc caaagggaca gtgtacaccg catggatcgc tcgccgcgag
      661 cgggtcaggc gccggtaatg ccttcgttgc ccgccatgcc gagcagcgtg ggctggttga
      721 gcctgtcgat gcggatcacc gtcttgtcgc gcaggtaacg gcccaccacg tcccacaccg
      781 cttcgccggt cgccccctcg ttgaccggcg cccagcccgc aaccttgtag gtcttggccg
      841 gatcgagcgg cttgcccctg agggtcaggt tgctgatgcg tttgccgacg gtctggttgg
      901 ggtcgcaggt gtattgcagc ccaccgacgc ggaccatgtc gcccccctgt tgcttgtacg
      961 ggtcggcatt gaacaggttg tcgcacacgt cttccatgat gctcttgatc gtggcgccgg
     1021 tcatgttgtt cagcgtggtt tgcggatagg tgatggcggt ctggtccatc aggtgttcca
     1081 tggtcacggc ctcgcctggc agcaatgatg tgccccagcg gaaaccgggc gagaaggcga
     1141 tttcggcatc cttgtcggcc atcagcgcat cgagaatcag ctggtcgaaa gtgccgttga
     1201 agttgccgcg ccggtagagc aggtcttcgg tgaccgcgag tttttcgccc aggcgtgcct
     1261 catagggagc ccgcatgcgg gtgatcagcg ccgccatgtc ggcatcggcc ggcagcaggt
     1321 tggcgaagat cggcagcagc ttgtaacgga aatcggtcac cttgccatga cgcacatcga
     1381 agtcaagcac gccaaggaat ttgctgttcg aacctgcgtt ggtcaccagc gtggtaccgc
     1441 cgctgttgcg taccagcgtg ggggacggca cgccatcgtg cgtgtgaccg cccagaatgg
     1501 catcgagtcc ggtgacgcgg ctggccagtt tgaggtcgac gtccatgccg ttgtgcgaca
     1561 gcaggaccac cacctgcgct cccttgccgc gcgcctcgtt gatgacgctc tgcagattgt
     1621 cctcgtggat gccgaagctc cagtcgggaa tcatgtagcg cgggttggcg atcggtgtgt
     1681 aagggaatgc ctggccgatg atggccaccg gaaccgaatt gatggtgcgc atcacgtagg
     1741 gctgaaacac cggatcgccg aaatccttgg tgtggatgtt ctgcgcgacg aaatcgatgt
     1801 ggcccttgag gtcattgttg accagttgca tgacacggtc agcgccgtag gtgaattccc
     1861 agtgcccggt catgatgtcc acaccaagca gcttcgccgc atcgaccatg tcctggccgt
     1921 gcgtccacag tgacgtgccc gagccctgcc aggtgtcgcc gccgtcgagc agcagcgctt
     1981 cgggacggct ggcgcgtata cgccggatca gcgttgccag gtgggcaaag ccgccgactt
     2041 tgccgtaggc atgtgcagct gcggtgaaac cgaggctggt gaacgcatag gcgtcgcggc
     2101 tgtgtgccgg aatgccgaaa tgtttcagca gatattcacc gaccaggtgc ggaggctggt
     2161 tccggttgtc gccgatgccg atgttgatgt tcggttcgcg gaaatatacc ggcagcaact
     2221 gcgcatgaca atcggtgatg tgcagcagcg atacattgcc gaatgcaggc aggtcgtaga
     2281 atgtatcgct gtgttcgccg gccagcgcct gacggttgtc aagcgccagg ccggcagctg
     2341 cggccgcagc caggagttgc atgaattcac ggcggttcat ggacatggca gatcctcagt
     2401 tcagggtggt ctggccagag tagctcatgc cgagattgtc cacggcgctg atgacgagct
     2461 gatcgccact tttggcgccc ttgaccttga attcgaaaaa cgggtttttc gacacgccga
     2521 tgccccattg ggcctgcatt accggggtac cattgagtgt ggcgctgacc aggttgatga
     2581 aatgggccgg aatcaccttg ccggcggcat ccttgcgcag accggtttcc atgtcgtggt
     2641 gaatcagtgc cttgatgtcg gcttcgccgt cctgcagatg ggcgcggatg agaatgggtt
     2701 gatccatggg tatgtccttg attgtattcg ttgcgagtcg gaaagtgtgc ggatatgggt
     2761 atcagccgcc gcagccaccg gcggtgacct tgatcgactt gctggtcatg tagaccttgc
     2821 catcggcctt ggccatgatc tgcaccgacg atgtctttgc catcttgatg cgtgtggcaa
     2881 tgaacggttc agcaccgttg agaaaagtga agtcggcgat cagcggattg gggttgtcgg
     2941 gtacgaaaat cgccatcgag gtggtgccgg gaatattgct gcttgcctgg accggtacga
     3001 ccgagccgtc ttcggcgatt tccggcaatt tgaggacgat gtcgctgctg ttgactgcat
     3061 tggcgtagcc tgagcgggaa tacgcatcgc tggccgtttt ggcggtgaac gcctggacgt
     3121 tccaggtggc ggcagcccag gcgccggcag acttcagcag gccgctgccg aaggcagccg
     3181 cggcggcggt gaacagggtg gcgcgttgca ggaagtttct gcgaggcaaa ttcattgttg
     3241 acctttcatc atgaacatgg gtacggggta aaaagacgat gcctgtcagg aagtccaccg
     3301 gctgcgcgtg gacgcaagcg caaacccggc ggtgacggcg taacggttac cggcttcagg
     3361 tgcgtacgta atcccagccc tggcgcacgc gccggttgat ctcttccagc gcagagccag
     3421 cgagccggat gtcgggcagc aaccggacct tgatgccctg ggcctgcagg gaggcaatgc
     3481 tctggttgca ggcataaaac tcgaggttcg gatagcgcgc ctgcatcagg ccgacccgtt
     3541 gcctgaacgg ggacacgtcg ctgcgcagca gatccagtcc gttgccgttg gcaagaatct
     3601 cgacttcgag cggctggcgg ttgcggcgat aggtatcgag cagattttcg gtctcgtcca
     3661 gcgctgcctt gagacggatc gggttggcgt tactgacctg gacgatgatc ttgtccgggt
     3721 tttgtgccat cgatccgctc tggatggtct ttagcagacg cgaggtgcgg ctgacgctgt
     3781 tcatcgtgtt tgcgtctgac agcatccagc ccgacgaccc accaaccagc agcagcacca
     3841 gcatgccgag cacgtattgt gtcatggtgc ctggccacgg acgcattctg cggcccgggc
     3901 ggttcatgct gactggtggc agcggatagg cgtgctgcat catcttcttc agcccgtgca
     3961 ggtcgcacac ccgttacttg agcgcggcat cctgttcgat ggaagtaaac aggcgcgcct
     4021 gttcgccggt ttcgagttca ttgtcgacga atgaattgag cagttcgtcc gaaacgggac
     4081 gctgtggtgt catttgaccc tcctgagagc gatcggctgg accgcatcct tggtctggct
     4141 cggggcgtgt tcgcgtagca gggtctgcag cgcctgacgc gcccggcaca gacggctcat
     4201 cacggtgccg atgggaatgg acagaatatt ggcgacttcc acataggaga attcttccag
     4261 gtcgatcagc gtgaccacct ggcgctgtcc ggtcgacagg cgcgagacgg catcgcgtac
     4321 ccgggtcacg atttcgttct ggtcgtgttc gacatcgggt gagcgcgaat gtgaaaacag
     4381 gctctcgtcc atgtcatcga tatcttccat ctcccggttg tgacggaaat ggtcacgcca
     4441 gcagttggtg aggatgctga acatccagcc atcgaaacgt tgcgcatcat taagctggcc
     4501 cagacgtttc agtcctttga ccagtgtttc ctgggcgagg tcttcggcca gggcggcatt
     4561 gcgcgtccat gagtaggcga cgcggtacaa ccgggggcgg atctgttcga atcgctgttc
     4621 gtattcatcg gcccggcaga acaaaggaaa cagtttcatg ctgtgcttca cccgaaaaaa
     4681 atgcggcgtt acggcataag acgcgagtcg ctccggaatt attccctgcc gcggtaaaaa
     4741 aacagactgc ggaataaagc cccggatccc ggcgtcctcc ccatgcagca attccaaacc
     4801 aaggagtgag ttacatgcac agtaccacaa gaaaactgac catacaggcc ctgatgctgg
     4861 tcgcgctgct cgcgccgggg gccggtcgtg cggatgcgcc cgcgctatcg ccgcagtcgc
     4921 aggcgaaaga acacgtggtg ttccaggtgt ccgatgcgtc tgcagccacc tggaacctca
     4981 cgctggccaa tgcccgcaat gctttgaaca gcctgggcaa ggatcatctg gaggttgaaa
     5041 tcgttgccta cgggccaggc atcgacctgt tgcgcttcga atcggtgctc ggcaacagta
     5101 tcggtcaggc agtcgccgac ggtgtgcata tcgtcgcctg ccagaatacg atgaagagca
     5161 aacatc
//
LOCUS       DAIANL010000095         5879 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1556005, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000095 DAIANL010000000
VERSION     DAIANL010000095.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556005"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1269 a         1807 c         1708 g         1095 t
ORIGIN      
        1 atgatgtcgt ccatagccgc ctggggtgcc tcgatgaaca cactgagcct taacggttca
       61 tgcacccagc gcttgccatc atgcagcgat tgcaacggca ggccaacgcg cagatcgccg
      121 ccgttaccct ccagcacgcc aatggctccg cccaccacat tgtgcagcac cttgttgccg
      181 ctgccgaagc gcttgttatc gaccacagaa cccaggtact gcatgttgat ccagttggct
      241 acgatcatcg gcgcggtcat gatcagttcc agtatctgaa agcccttgtc agcttgccac
      301 tggtattcat gcaaaaaagc gcggccaccc agatccatgc cggccgtacg gctgcgtggc
      361 gcggcaatga aggcggcgtt attggccagc gcccactccg gtcgcacctg ggaccaatcg
      421 cgactacgct ggcacaaatc ggtttctatc gctttgccgg gcaggctggc aatcccgagc
      481 atcgcagcgc gctgcatgcg ggtcaggtcg ctggcttgct caagccactg gtgcaggcgc
      541 gcgaggtcga cagcgagcga ttgaggcata tcatccgtat cgaacacgcg gacgacatcc
      601 gtggtggtgt cgtgaagccc ggccagaaac caggtgtcct cgggaatcat gatgccccga
      661 tcgcgcaagt cgcgccgcac cgagggctga ttcagcaagg aggccaccac gcgagcgctg
      721 acctcgccgg tttgtcccgc gcaggccccg caatccaggc cggtggcgtg cgggttgttc
      781 accgaactgc tgccgtgccc catcaacagg accagacgcg cgaagttgtc agtcagggac
      841 atggcgcgca ggatgcgctc ggcatgcccg acccagtcgg acggcggaat gccagaatcc
      901 gcatgattcg ggcagcattg cgccccggga acttcagcca ggctcgggcc gatgcggtgc
      961 atgagttgcc ggtccagccc ctgggtaaag gggtcgggca ccggccggct ccagccaaag
     1021 ctgtccgtca acaattttgg cgcatacatc aatccggccg cttcgacaaa agaaaagcag
     1081 gaagacgccg acagtttgaa acccttccac actttggaca aacccaaacg gcgccgccga
     1141 ccgattatcg ctctgaccgt ctgctgatcg ccatccttga ccttgtcata aatgcgatag
     1201 gccggattga agatcaccgg cacatggctg tgcccggaat ccgcacccag tggcacgtgc
     1261 tcgatgaaaa taccgaaaaa ccctgcaaag ccaatggtct ggaccgatcg cgccacagtt
     1321 tccagcgaac ggcgaaacac ttcggaacgc acgtcgatgc agaatgccgc ctgaaccgtt
     1381 gggcgcatgc gcgggctagc attcgcctcc ttgttcaggc cggcgattac cctgcgctga
     1441 aaaccgattt ccatggcatt caacatgatg cgatcaattt ccgcggccac ttgacgcttg
     1501 gtggacggag gacgcatgct ggctgccagc atgtcgcgcc agcgcgccac cagtgcttcc
     1561 gatttttttt ccttgaacag cagcacatcc cacatcagac gaatggccag caagtcgata
     1621 atcgaatcat cgcgtttgcc gccgagttct gcctgccagc gcaggtaacg cgcccaggat
     1681 gcccagccgc cgatgctcag cagcgatgcg tgcagataac gctccattcc tgcctcgggg
     1741 atatccagct gggcaacggc ccaggcgatg gcggcacgcg ggtcggaggg cagatggctg
     1801 atttccgaac gaaagtggtg caagcccatc atcgccggac tgcggtcaat ggcggcagcc
     1861 ttgagccagg atgcatagag cgactgatca caccagggca aggccacgat cgcttgaccc
     1921 agatcaaagt aagccgcgca atgcaagctg atacgctcgg tgacaaaact tgaccacaga
     1981 ccgccttcca cctgttccag cacctcgctg accggagcca tgcccaatct cgggcgaggt
     2041 gccgcagcgg acagcgtcca ctcgacgatc gcgatatcca gctggctgtc gcagcgggca
     2101 atggcctgct gcaagtcttc acgcgtgatg cggccatttt gcagctgctc gcggtaataa
     2161 tcgcgcggca tgtacagatt ggagcccgtg atccgggcca gcgtgtcgga agcctcctga
     2221 aaactgtggt cactcagacc aaagaaagga ttgacggcca caaagtgctt caacggccac
     2281 agcggcgcga tgcgtccgca ggccgcatcg atgcaccggt tcagttcggc agcatccggc
     2341 accgcccgcg cgggcgcgcc ggtttgcatt gccggcactg cagaaaccac acctccggta
     2401 gcgctcagtg ccatgctgtc gtttgcattc atcatccacc tccctggggc ttttctgcgg
     2461 caaagggcgt aggcaggctg cccgataccg ggcttggcca tatccgctgc agcagccggg
     2521 taatgtatac gtcaatgtac agaccgttgt agagatgcat gtagaggccg cccaccagac
     2581 ttgccggagc gtatttgagc accttctgca acatcagcag catcaggaaa ataccgacga
     2641 tcaacgcagc cagtgcgttc tggaaagggc cggccgcact ctgcaagggc agcacactgc
     2701 cttgcagagc catctcgaac agggcaggca agctgaaata ggccgtgcag accacggcac
     2761 tcaaacccag cccgcgcaat acgaatatgc cactgttcat cacgctgacc gtctgcagca
     2821 acagttggct catggcgatg gcgacgacgg cacctgtggc catcagcgcc ggttgtgact
     2881 gcagggtgat gccaaacacc gaacctacgc ctacggtcat gcctcccccc gtaatgagcg
     2941 caatggccat gcgccagaat ttgaagttgc cgtgattgcc cggcatggtg ggcgcacgaa
     3001 aagcgtccac accactgccg gaagcgagga aggcgtgcgc cttgtacagg gagtgggcca
     3061 ccaggtgcat catggcgagg ctgtagaggc caaggccgca ctccatcaac atgaagccca
     3121 tctgcgcagt ggtagaccag gccagcgaca ccttgatgct ggtctgcgtc aacatcacca
     3181 gcgaggccag tgccagggtt acgaggccga tcacagcgag ggttcccagt gcgatggccg
     3241 agtgcgacat gatggggctc atgcggatga tcaggaaagc accggcgttg acgattccgg
     3301 catgcagcag cgcggacacc ggagtaggcg cctccaccac ctggatcagc cagccatgga
     3361 gcggaaactg ggcagacttg agcacagcgc tcagcacgat cagcagcgac gcccattgca
     3421 gggccagcgg caaggggcct tgcagcgccg ccatgctgcg gaacatgtcc gaatactcaa
     3481 ggctgtgcaa gttcgatccg atcaggacaa tggccgtgag atggctcagg tcactgatgc
     3541 ggttggcaat gaatttcttg tgcgcggcca gctgggcggc ccggcgctcg cggtaaaacg
     3601 tcagcaactg gtgcaggcac aggctgttgg caatccaggc cagccagaac atgagcatgt
     3661 tgcctgagat gatcaacgtc aggatggtgc ccagcgtaag gctgagccat ttatggaaac
     3721 gcccctgatt ggaatcacca tcgaggtaat tgcgcgaata gcgtgtcacc acggcaccca
     3781 caaacgacac gagcaccagc atgatcacgg tcacattgtt gacgtaggcg gtaatcgaaa
     3841 aggcgccgat gtttcccggc aagtcaatgg cataaagcgt ccaggcacgg gtgccgtcga
     3901 agctgtgggt cgcggccgat aacagggcgc tgatgcagga aaaccaggca atggtgccgt
     3961 taaggcgcgc catgaaccgc gcacgctgat tggcccagcc cgtcggcaac aaccccacca
     4021 gcaacaatgc cagcggagcc gacaggacaa gaatgggggt caacaggcca gacagggttt
     4081 gcatgacaat ctccgttcgg gggttgcaga cgggcgaagg acatcctgtt ctttcaccca
     4141 cggatagcgc acagaatcct ggcgagacat tgccgcacag cgcacggcgc ccggcttgtc
     4201 ccggaaatcg ggcgatggca ggcgggaatg aactccggcc atggacacat cactgcgcga
     4261 caacaccatg ttcgacgcca cggcatgcaa gtgcatcacc cctgctcgtt cggcattcat
     4321 cgtgtcaatc ccatataaat cacgggcagc ttttccggca agcgctgtat gtgatcaacc
     4381 accatgtaat ttctcgcacc gaagatgcgc gacacatact gatccgcgcg tggatccagg
     4441 ctcaggcagt aggtcgatat gccgtcacgc cccaaggctt ccaccgcttt tttggcgtcg
     4501 gcgcgcagat actgcgggtc gcgcacatcg ttatctgcgg gctcgccatc ggtaatgacg
     4561 agcagcagtt tcttgttgct gggctggcgc ttcagtatcg agcctgcatg acgcatggcc
     4621 gcccccatgc gcgtggacat ctgaccgctc attcccgcca gtctggcttt ggcctgatcg
     4681 ttgtatggcg caccgaaatc cttgtaccgg aaatattcaa tgtcatgccg gccatttgaa
     4741 tcgaaaccgt gtattgcgaa cggatcaccg atcttgtcca gagcatcagc gagcagcacg
     4801 gccgcctcgc gcgcaagttc cagcacggtg ctttccgaac cgaataccgc atcattggtg
     4861 gattcggaca agtcgatcag caacaacacc gacaagtcgc gtagtttgcg cacgttgcgc
     4921 atcatgatac gaagatccgg gttttcgccc atgcggattg ccgtcatcgc ctgcacagcc
     4981 gcattcaaat cgagttcatc gccgtcttcc tgtttacgca agcgctgcac gccttgcggc
     5041 tgcatggctt cgatcaggta cttcaggcgg ccgacgatgg gtttgtgctt gacgacgatg
     5101 tcgtcaagca gcgcgatatc gccaggcttt gcacgcttct ccagcaccgt gcaccagtcg
     5161 ggccggtcca gttgcatctg ataatcccac tccgggtaat ggtaggggtc cggcggcggc
     5221 tcgcggcctt cctgctggtt caggctggtg gtttcttcat cgcggaaaaa ttcgctggcg
     5281 agtacccaga tttcattggc atcatcgccc gctccgggca cgtcgatggc attgatcatc
     5341 tccatcacgc tgacgtattt gcggatttgc cgcgaagtaa cacctgcaat cacctgtccg
     5401 gtttcgcgca gatcttcaaa ctcccacatg tagcggttat catcgcggta aaaaatatcc
     5461 gccacatcgg cagactggct gtaactgacc cccaactcga gtaacttgtc agccagcagc
     5521 acgccgagtt cctttaccca gccaacccca tcggttcgtg cacggtattc atgaaacaga
     5581 tgacagccga gcatcaccca gggatgatcg tcacggtagt cttcgtcgag caggatgcgc
     5641 gcaagtcggg ccatcaacgc cgcagccgtg tcacctgttt gcgggttggc cgtgtgctgg
     5701 tacagccaga tctgcctgag accggggaat tgtgccacgg ccagaccttc cacacgggca
     5761 tcctcgatca ggccgaccaa cgccacctgc agcgcactca gtccggttag gtcgaactgg
     5821 gcggtggtgt acatttgatg gcaggctgca tgggctgcgc tggcacgata caaatcgat
//
LOCUS       DAIANL010000096        10681 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1956312, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000096 DAIANL010000000
VERSION     DAIANL010000096.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 10681)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10681)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10681
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956312"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1895 a         3022 c         3518 g         2246 t
ORIGIN      
        1 ccgaccagca ccaccggagc agcgccacgc ttgcccccct ggtactgcag cacgatgagc
       61 ttgggcggct ggcggctgcc ctgggctacg gcgagcagag cgcccatgcc cagtttttcc
      121 atatccttgc gttcgagcac ctcgcatttg accggcaggt cgcgggcgat cttctgtgcc
      181 tgctcggcaa ggtaggttgg tgtgcagatg ttgccgggca ggttgccaag gtccttggcc
      241 agttcggcgc catcggcgat ggcctgtgcc aggtcgcacg cagccgcggc cgattttgcc
      301 gacgccgcgg ggacgatgag tgccagtttc ttgagggtgt ggccgggtgt gtccggtccg
      361 gttttgagtt tccccggaac aaaacgctgc gcacgtaccg ccagcaccgc ctgttcgatc
      421 tgccattcca cgctgcgcag cgctaccgtc tcttcgaaca ggtagagcgc ggcgctggag
      481 gcgccactgg agcccagcgt gctgcaggcg gcctgcaacg cactgagata gcttttgcca
      541 tcgagttcgt ttgccttgcc cagccccacc agcagcacgc gttcgctgtg tatgcctggc
      601 acgtggtgca gcagcagtga gctgcccgct tttccgtcga ggtcgccact ggcgagaatt
      661 tttcccagat aaccattgct caggctatcc aactgctggg cagtggcgga taattgtcgc
      721 gattcgtaga cgccggcaac gatgcatgct gcgcgctgtt tttcgggttg ggtgcttttt
      781 atggtgaatt ccatcggacg cctcggctat actgttgcaa tctggtctgg tgtacgcccc
      841 cattatttgc agtctttgtc caaaagtcaa aatacaagtg gttcgtcatg ctttttcgtc
      901 gcgccttgct gcgtgaactg attttcaatt atttcggcgt gttcatggtg ttgttgacca
      961 tcaccctgac cacgttgttc atccgtctgc tcggtgatgc agcacatggt gagctggccg
     1021 tggacgccgt gctggctttt ctcggctttg cgctgttcaa ttttctgccg ctgatcctgt
     1081 cgctgacgct gttcatcgcc attctgatga cgctgtcgcg tgcctaccgc gagagcgaaa
     1141 tggtggtgtg gtcgagctca gggctcggtc tgggggcatg ggtgaagccg gtgatgacat
     1201 tttccatgcc ggtggtgctg accattgcca tgctcaattt cgtcatcatc ccctgggcgc
     1261 tcgacaagaa ggacgaattt cgccagttgc ttgatcgtca ggacgaactg tccgtgattt
     1321 cgccgggcat gttcaccgag tcaaaacagg aagacaaggt ctattttgtc gagtcgctgg
     1381 ccgcgaacgg cgcgcaggtg aaaaatgtgt tcgtgcagtc ggtcgacaat cagcagctcg
     1441 ggatcacggt ggcgcagaca ggcgttcacc gccagctgtc caatggcgaa cgctatctgg
     1501 tgttgcagaa tgggcggcgc tacgaaggtg ttccgggtca ggcggattac aagatttccg
     1561 aattcggcga atactggatg cgggtgcagc agcagaccct gacattgcat caggatttcg
     1621 gcgccaatgc caaatccacc gccgaactgg tcgctgatcc gacggcggcc aacctggccg
     1681 aatttgcctg gcgttgcggt ttccccatca gtgcgacggt gctggcgctg ctggcgattc
     1741 ccctcagctt tgtcaatccg cgcgccgggc gctcattcgg cctgatcctc gcgctgctgg
     1801 tattcgtgat ctacaacaac ctgcttggca tcgtcgaagc gtggatcgca cagcagaaac
     1861 tcggtttcgt ggccggcctg gcgagcgtgc atggcgtgat gatcgtactg ctgctgtgga
     1921 tgttctggca gcggatcagc atgcgcagcc tgaggtggtg gtgatgcgca tcatcgtccg
     1981 ttatctgacg gtccagatcc tgtcctcgtc gctgttcgtg tttgcggcgc tgaccacgct
     2041 gttcgccctg ttcgacctga tcaacgagct ggattcgctc ggcaagggcg cctacgggtt
     2101 ctggcacatc ctgttgtatg tgggcctgaa cgtgcctggc catctgtacg aactgctgcc
     2161 tgtggccgcg ctgatcggca gcctgctggc gctcgcccgg ctggtgagca actccgaatt
     2221 caccatcatg ctggcttcag gtctgtcgat gcggcgcatc gccgtgtaca tgatgctgat
     2281 cgggctgctg ttcagtgcgc tgacgtttgt gttcggtgaa atcattgcgc cggcgacgga
     2341 tcgctatgcc gagcagatga agttgcgcgc cacgcacaag ctcgtggccc aggcgttccg
     2401 ttcggggctg tgggtgcgcg accacaacca gttcgtcaat gtacgtgaag tgtcgcccga
     2461 tggcgggctg cgcgatgtca gcgtgtacgt gttcgatccc cagtttcatc tgcagcgcat
     2521 ttttcgtgct gccagtggcc agtttctgca cgacaacctg tggcagctga gcgggctgga
     2581 cgagaccgat ttcctgcaga acggcgtcca ggtcaggcac ggtccggcaa cggtgtggca
     2641 gtcggtattg acgccggcca tcatgaatgt gttgctggtc gctccggaaa agatgtcggc
     2701 gcaggacttg tgggcgtatg tggcgcacct gcacgccaat caccagccga gcgagcgtta
     2761 tgaaatcgcg ctgtggagca agctggtcta tccgctggcg gccccggtca tgctgctgct
     2821 tgccattccg tttgcatggc accggccgcg cgccgggtcg gtcagcacgc gggtgtttgc
     2881 cggcatcatg gtcgggctga gctttcatct gttcaaccgt ctgtttgcct acatcgggtt
     2941 gctcaatggc tggcagccat ggatcagcgt gtcgttgccc acgttgctgt ttctggcagt
     3001 gggcgtgggt ctgctgttgc gcgtcgaacg ccagtaatgc ctcagcgtgc ctgcaccaga
     3061 cgggtgccgg caatgcggtc gtgcaggaat tggtggtcgg catcgaacag cgcccacagc
     3121 tgactgatgc caggcaatgc aagtgccacg atgaagcgca gcgcggcctg taacggcagc
     3181 aggcggccgc catcgatgcg ttgcagacgg atgcgccagg ttttcatcgc aagtgactgt
     3241 ccgtgtaacc acatccagct gaaatagacg cccatcacca tgaacagcca tgcctgcagc
     3301 gcgcgttgga gccattcggc atgcacgttg cgggtcaggg tgatgaacag gtagtcggcg
     3361 acgaacagta cggcggcgag cagcagcagt tcataaaaga tggcagcaag gcgtttgggc
     3421 agcgaagcgg ttggggcatc agactggggc atgggcaggc tcggcagaat tttatggcgt
     3481 cattatccac cagttttgcg cgctggcggg cgaacggttc aaattgtcga ccaatttttt
     3541 ctggatcatc atgcgtattg gttcctgggt actgaaaaac aatctgctgg tcgcgccgat
     3601 ggcgggcgtg accgacaggc cgtttcgcca gctgtgcaag cggctcggtg cgggcatggc
     3661 tgtctcggaa atggtcacat cgaattcgct gttgtacggc tcggccaaga cgcgccggcg
     3721 tgccgatcac gaaggtgaag tcgatccgat ttcggtgcag attgccgggg ccgatccgca
     3781 gatgatggcc gaggccgcgc gctacaacgt cgagcgtggc gcgcagatca tcgacatcaa
     3841 catgggttgc ccggccaaga agatctgcaa cgtgatggcc ggttcggcgc tgctgcagga
     3901 cgaggcgttg gtcgcacgca tcttgtccgc ggtggtggcg gcggtgccgg ataccccggt
     3961 gacactgaag atccgtaccg gttgggacaa acagcaccgt aacgcgctga acattctcaa
     4021 cattgccgag aattgtggcg tacaggcgct ggccatgcat gggcggacgc gcgcctgcgg
     4081 ttattccggg cacgcggagt atgatacgat agcggctgtc aaagcggtgg cccggattcc
     4141 ggtaattgcc aacggcgaca tcaccacgcc ggaaaaagcg gcttacgtgc tcggacatac
     4201 gggcgccgat gccgtcatga ttggcagggc tgcgcaggga cgtccgtgga ttttccgcga
     4261 aatcgaatat ttcctgcgta ccggccaacg catggcttcg ccgcagctgg gggagattca
     4321 cgcgatattg cgtgatcatc tgcatgatct gtatggattt tatggcgatc tgaccggggt
     4381 gcgtgttgca cgcaagcaca tttcctggta cacgcacggc ctgcgcgatt cggcgcgatt
     4441 ccggcacgcc atgaatcagt tgcagaccgt ggaggaacaa ttgctggcag tggatacgtt
     4501 ttttgaacac tgccggcgct acggcgagca tttcgatgat gccgcgatcg ggtctggaca
     4561 tccggaacaa ccggtgcagg gtgaaatgga atgatcaaac agggtgaagt ggcggcctgt
     4621 atcgaactgg cgctggatac ctttctgaaa gacctcgatg gggaggtgcc gagcggggtg
     4681 tatgacatgg tgctcagctg tgtggaacgg ccgctgattg aaaaaatcct gcattatgcc
     4741 gagggcaacc agacccgcgc cgcggagtac ctgggtatca accgcaacac gctgcgcaag
     4801 aaaatggcgc aatataacct gcagtgacgt cgacgacgtc gctggacgca acttcaggaa
     4861 cgaatcaaac atggctcata tcaaacgggc gctgatcagc gtatcggaca agaccagcat
     4921 cgtggcgttt gcgcgcggat tgcgcgaaca gggggtggaa ttgctgtcga cgggtggcac
     4981 cgccagcctg cttgccgcag agggcatagc ggtgacggaa gtggccgact ataccggttt
     5041 tccggagatg ctcgacgggc gcgtgaaaac gttgcatccg aaaatccatg gtggtctgct
     5101 ggcgcgtcgt gaccgggccg aacacatgga agccatccgc gcagccggga tcgaaccgat
     5161 cgacctggtg gtggtcaacc tgtacccgtt tgttgaaacc gtatcccggc cggattgccg
     5221 gcttgcggat gccatcgaaa acatcgatat cggcggtccg gccatgctgc gttcggccgc
     5281 caagaatttc cagcacgtgg cggtggtgac cgatcccgcc gattatcagc gcgtgctgga
     5341 ggagatgagg cgcaaccagg gttcactggc cacgtccacg tgctacgatc tggcgcgcaa
     5401 ggcgttttct cacacggctg cgtatgacgg cgccattgcc aaccatctta cctcgcatga
     5461 gctcaatggc gagcacagcg cgtttccgtc acacctgagt ctgcagttcg agcgcgcgga
     5521 agtgctgcgc tatggtgaaa acccgcacca gcgtggggca ttttatcgcg acgcggttcc
     5581 agctgccggc agtctggccc gttacagcca gttgcagggc aaaccgctgt cttacaataa
     5641 cattgccgat ggcgatgccg cctgggaatg cgtcaagacg ttcgagcggc cggcctgcgt
     5701 gatcgtcaaa catgccaacc cgtgcggcgt ggccgtcgcc accgagccat tgcttgcgta
     5761 tcgccatgcg ctggcgacgg acccggtgtc ggcttttggc ggcatcatcg ccttcaattg
     5821 cgaggtggat gccacgacgg ccgaagcggt gtgcggccag tttcttgaag tgctgattgc
     5881 gcccggctat agcgaacagg cgcgtgccgt gctggcgcgc aaggtgaatg tgcgcgtgct
     5941 gctcgtgccg ctggcgcatg aatcgaatgt actcgaattc aaacggatcg gcggcggact
     6001 gctggtgcaa ggcccggaca atcacaacgt gagccaggca gaactgcgcg tggtgacgcg
     6061 ggtgcgcccc gaaccgcacc agtttgccga cctgctgttt gcctggcgcg tggccaagtt
     6121 cgtcaagtcg aatgcggtgg tgttcgccac cgatggccgt acgctcggca tcggcgcagg
     6181 gcagatgagc cgcgtcgatt cgacacgcat tgccgtcgag aaggctggtc aggcagggct
     6241 gtcgctgcag gggtgtgtgg tggcgtcgga tgccttcttc ccgtttcgcg acaatgtcga
     6301 cgtcctggcg cagtcgggtg cgcgggcggt gattcagccg ggcggcagca tgcgcgacga
     6361 cgaagtcatc gccgcggcgg acgagcatgg cattgccatg gtgtttaccg gataccggca
     6421 tttccggcat tgagtgtaac gatatgaacg tacttgtggt tggatcgggg gggcgcgagc
     6481 acgcactggc ctggcggctg gcgcagtcac cgcgtgtggg caaggtgttc gtggcgccgg
     6541 gcaatgccgg cacgcaacag gagaaggaac tgaccaacgt tccggtcagc gagccgcgac
     6601 tgctgctcga atttgcacgc cgtgaacaga tcgccctgac cgttgtcggc cccgaagccc
     6661 cgcttgctgc tggtattgtc gacctgttcc gtgaagcggg gctgaaaatt ttcggaccga
     6721 cgcaggcggc cgcgcagctg gaggcgtcga aggattttgc caaggcattc atgctgcgtc
     6781 acggcattcc taccgcaggt tatcgtacct tttccgatgt acaggccgcg catgactgga
     6841 tcgatgccca gggcgtgccc atcgtcatca aggaggacgg tctggcggcg ggcaagggcg
     6901 tggtcgtggc gcacacgctt gaagaagcgc acgatgcggt gaccaacatg ttgcaggaca
     6961 accggctcgg cgacgccggc gcgcgggtgg tgatcgagga atttctgcaa ggcgaggaag
     7021 ccagtttcat cgtcatgtgc gatggtgaac atgtgctgcc actggcgacc agtcaggacc
     7081 acaaacgcat cggcgatggt gacagcggcg acaataccgg cggcatgggt gcctattcgc
     7141 cggcaccggt ggtgacgccc gaaatacatg cccgcgtact gcgcgaggtg atcgctccgg
     7201 taatgcaggg catgaagcag gaaggttcgc cttacaccgg attcctgtat gccggattga
     7261 tgatcgatgc ggcaggtggt atcaaggtgc tcgaattcaa ctgccgcatg ggcgatccgg
     7321 aaacccagcc gatcatgatg cggctcaagt ccgacctggc cttgctcatg gagcatgcgg
     7381 tgcagggtac gctggatcgc gtggaagccg aatgggaccg gcgcgttgcg cttggcgtgg
     7441 tgctggctgc ggccggatat ccgcagtccc cgcgcatcgg cgacgtgatc gaggcattgc
     7501 cgtccgatga ggaggatgtt cacctgtttc acgccggcac acgactggaa gacggacggc
     7561 tgctgaccag tggtggccgg gtgctgtgtg tgaccgccct gggcgacagc gtcaagatcg
     7621 cccagcgtcg ggcatacgaa gtggccggac agattcattt caacggctgc cagatgcgcc
     7681 gcgatatcgg ttatcgcgcg ctggaacgca aaaaaagtct ttgagtatgc gcctgcggcc
     7741 gggagcggaa accgtgctcg cctatccgac cgaatcctgt tacggattgg gttgcgatcc
     7801 gctggccgca cgtgcggttc gacgcatttt acgtctcaaa cagcggcccg agcgcaaggg
     7861 agtgattctg attgcggcag atgtgcgtca gttggtgccg tttgtgcagc cgcaggcatt
     7921 gcgggaagcg ggtatgtccg gcatctggcc ggcaggcgtt acgctgctgt tgccggcttc
     7981 gcggcgctgc ccgtcatgga ttcgcggtcg gcacggcagt gtggcggtgc gggtgaccgc
     8041 tcacggtgcg gcacggcgcg cctgcctgct gtgtgggcat gcgctggtgt ctaccagcgc
     8101 caatcgggca ggacagcggc cagcgcgcac ggcgcgtgaa atcgagcgca ggttcggcgc
     8161 tcaggttcgt cgcctgccgg gccggatcgg caaagcgcgc cggccgagcc gcatcgtgga
     8221 ctggcgcagc gggcgcgtgt tgcgcgaatg aggggatgaa catggtgaat accgcaaccg
     8281 tgcggtcggc gttcctggca ttgcagacgc tgatcgtcga acggatcgaa cagattgatg
     8341 gcacgtcgtt tcgacgcgac agctggcagc gcgatgcggg tggtggcggc gtcacctgcg
     8401 tgctggaaaa tggcggtctg atcgaacgtg gtggcgtgaa tttctcccat gtatacggtg
     8461 atcatctgcc accatcggct tcacaggcgc accccgaggc ggcaggccgg gcgttcgaag
     8521 ccatgggcgt gtcgctggtg ttccatccgc gcaacccgca tgtgccgaca gttcacatga
     8581 acgtgcgctg tttcgttgcc ggcgcgggcg aggaaaatcc ggtgtggtgg ttcggtggcg
     8641 ggatggatct gacgccgtat tatggctatg aggaagatgc cgtgcacttc caccgtattt
     8701 gccgcaatgc gctgcaaccg ttcgatgcgg gttattatcc gcgtttcaag cgctggtgcg
     8761 acgagtactt ctttttggcg catcgtaacg aaccgcgggg catcggcggt attttcatcg
     8821 acgatttcag cgaacgcgat ttcgatcacg catttcagac catcctcagt gtaggggatc
     8881 acttcctgcc cgcctgggtg cccatcgccg aacagcgccg ggccatgggg tatggcgaac
     8941 gcgaacgcga gtttcaggcg ttccggcgcg ggcgttatgt cgaattcaac ctggtatatg
     9001 atcggggcac gctgttcggc ctgcaatcgc gtggccgcag tgagtcgatc ctgatgtcca
     9061 tgccgccgct ggctcgctgg aagtatgact ggcatgccga accgggcagc gccgaagcgc
     9121 gactgctgag tgattttctc actggaaggg actggcttgc cggagtcgat tgaaggaacc
     9181 accgcgctac gcgtcgtgcg tgatgttggg ccaggcacag cgagtgtagc cgccaggtgc
     9241 cgggctgaca cgtttccttt tcgttgccga tcggcaggta cagccgggtc gggcgctcct
     9301 gtagtcgttg aaaccgtgcg tgcaggggtt tcgtgtcgcg gggacggttc tgtgcggggc
     9361 attgcatgac cggcggcagg ctggcctggt tcgttgccgg tgcgggggtg gtcgttctgg
     9421 cggtgctggc aggtttggtc gtgcgcgagg cgctgacgtt caatctcgaa gcccgttacc
     9481 tgagccggga ggcggcctcc atgcgttatg gtgctgaacc tggccccagc ccccgcatcc
     9541 gatttccgca gttcgggcca gccgatgagc gtctgggtta caccttgctg ccggaatttt
     9601 cccgccggtt gggtatgcag gggtatgcat gcgttcgcca ggcgcgtatc agtccgcaga
     9661 tggctcattg ggccgacctc ggctacaacc tgccctacca cgaaaaaacc cggggcggat
     9721 tgcgcctgct ggatgatacc ggggccacgc tgtacgaaca tctgcagcca cggcgtcagt
     9781 tttccggttt cggcgacatt cctgccgagc tacgtgacag cctgctgttc atcgagaatc
     9841 atgaattgct ggcgccgcac ccgcgggatc gtaatcctgc cgtggaatgg ttccgtctcg
     9901 tgcaggcgct gatcgacaag ctcgagcagg aaatcttccc tggccatgac gtgccaggcg
     9961 gcagtacgct tgcgacgcag atcgagaagt ttcgtcattc ccccaacgga ctgacgctga
    10021 cggtgcggga caagtggcag cagatggtgt ccgcgtcgat gcgggcgtat ctcgatggtc
    10081 cggacacgct gaacgcgcgc cggcgcctgg tgctggatta cctcaacagc gtgccattgt
    10141 cggccacggc cggatttggc gaggtcaatg gcctgccgga cggcatgtgg gcctggtatg
    10201 gactggacta tcgtgaactg accgaacggt tgcgtgcaca gcgcatcgac gcgcgtacag
    10261 gcatgctgtt caaacacgcg ctgtcgctgg tgatcgccca gcgcaaacca tcgtatttcc
    10321 tgatctccaa ccgcaatgca ctcgatgcgc tcaccgattt ctatttgcgc ctgctggcca
    10381 ggcgtcaggt gatctcaccg cagttgcgcg acgttgcatt gcagtccgcg ctgcggttca
    10441 gaccgacggc ggcagtgcgg ccgcgtgatt tcgtcgcgca gaaggctgcc aacgcattgc
    10501 gcaaccagtt ggcgacactg cttggcgtac cgagcctgta ccggctcgac cagatcgatc
    10561 tgacggtgaa taccgaattc aatgctgccg tgcagcagcg cgtcacccga tttctctcca
    10621 gcttaaatga tgtgcagacc tcgcagcaac tgggcctgta cggatggcat ctgttgcagc
    10681 c
//
LOCUS       DAIANL010000097        31267 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2983901, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000097 DAIANL010000000
VERSION     DAIANL010000097.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 31267)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31267)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..31267
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2983901"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5867 a         8526 c        10249 g         6625 t
ORIGIN      
        1 cctatcggca agggattttc ccctggttca atccagggca gccggtgctg tggtggtcgc
       61 ccgatccgcg catggtgctg ctgccggagc agttccacct gccgcattcg ctcgaacgga
      121 cactgcgcaa acgtcctttt gaaattcgca gcgacagtgc gtttgaagcg gtgatgcgcg
      181 gttgtgcaca accacgggat gggcaggtgg gcacctggat cgatgaaacg atgattgctg
      241 cttaccgcgg cctgcatgcg atggggctgg cccacagcat cgaggcctgg gcggacggcg
      301 aactggtggg tggcctgtat ggcgtggcgc tcggcagggc gttttttggc gaatcgatgt
      361 ttgcgcggcg cagcgacgcc tcgaagatcg cgctgacgca tctcgtgcgg cgcatgcagc
      421 agatcggttt tgaactgctt gactgccaga tgtacacttc tcatttggcc cgtttcgggg
      481 cgcaggaaat tccgcgtgcc gaattcggtg aacgcctggc gcgagcggtt gctgctgcgg
      541 cgccggcttg gcagacaggg gtgatgcatg acggatatgc atgatgacgc attggtccgg
      601 ctgcagtttt atctgacggg cgaacatgtg tgcagctatc ttccggggca gcaggcgcga
      661 tcccaggtgg cgacccccgc gcacaaggtg aatgctgcag tgtacggcga gttattacgg
      721 catggttttc gccgcagcgg gttgcatacg tatcgccccc actgcgacaa ctgctcggcg
      781 tgcgttccgg tacgcgtgcg cgtggctgat ttcaggccca atcgtaccca acggcgggtg
      841 cagcagcgca atgccgattt gcgtgcaagt ctggtgccac cggtgtttga cgaacagcat
      901 tttgccttgt atcagcgtta ccagaacgcg cgtcatgccg gaggcggcat ggaccacgac
      961 gaccgtgagc agtacgccga gtttctgatc gccagccatg tcgacagttg gctgctgctg
     1021 tttcacgaag gtgacgccct gcgcatggtc agcgtgatcg acagcgtcgt cgacgggctg
     1081 tcggcggtgt acacctttta tgacccggat tttccgcagc gcagtctcgg ggtgtacaac
     1141 gttctgtggc agatagccct gacacacagt ctcaacctgc cttacctgta tctgggttac
     1201 tggatcgaag gcagccgtaa aatggcctac aagagccgtt accagccgct ggaagggcgt
     1261 ctcgacggcg aatggcgcga cttggggcag accggcaaca tgacaaaaac aggatgacgt
     1321 gccgcaacgg cacgggaatt cattaccagg gaaccagact accatgcgtc aatatctcga
     1381 cctgatgcgt catgtgcgtg agcacggaac gcaaaaaacc gatcgtaccg gcaccggaac
     1441 attgtcggtt ttcggctatc agatgcgttt cgacctgact cagggtttcc cgctgctgac
     1501 tacaaaaaaa tgtcacatgc gttccatcat tcacgagttg ttgtggtttc tcaagggtga
     1561 caccaatatt cgctacctgc gagaaaatgg cgtcaccatc tgggatgagt gggcggatgc
     1621 gcaaggagac ctcggcccgg tgtacgggcg ccagtggcgc agctggccga ctgcggatgg
     1681 tcgccatatc gaccagatcg cgggtgtgct ggaacagttg cgtaaccagc cggattcgcg
     1741 tcgcatggtg gtatcggcat ggaatgtggg cgagttggat cgcatggcgc ttgcaccctg
     1801 tcatgcgctg ttccagttct acgtggccga cggcagactg agctgccagc tgtatcagcg
     1861 cagcgccgat atttttcttg gcgtgccgtt caatattgcc agttacgcgc tcctgaccat
     1921 gatgattgcg caagtgtgcg gattgcagcc gggtgatttc gtgcacacgc tgggggatgc
     1981 ccatgtgtat ctgaatcatt tgcaacaggc cgatgagcag ctggcacgta cgccccgtgc
     2041 gctgccgaca atgatattga atccggacgt ttcggatttg tttagttttc attacaatga
     2101 ctttacattg caggggtacg acccgtggcc gccgatcaag gctccggtcg cagtctagaa
     2161 cgataacaag cggggataga tccgcgcgga ggcaggggtg atcgattaca gcaaaagtcg
     2221 cgtgttgatt gtcgatgaca gtcaggccat gtgctcgtcc ctgcgcatca ccctgagcaa
     2281 ttttggcatt acgcgcagcg aatcatcgaa ctcggccggc gaggccatct ttcgcattcg
     2341 tcaccgcgag ttcgacatga tcgtctgcga ttacaatctt ggtgattcga ccgacggtca
     2401 gcagttgctg gaatacgtgc gtcgcgaaca cctgatccag ccgtcagtgg tgtttctcat
     2461 ggtgaccgca gagcgcagct atgaaaaagt cgtgttgtgc gccgagatgg cgccggacga
     2521 ctacctgctc aagccgttta ctgccgaaac catgcgggtg cggctggaaa aagtgttcga
     2581 caagaaagcc gtgttttcca ccatctacgc gcatctggcc gaatcccgtc tggccgaggc
     2641 cgtcgaggaa tgcgaccggc tgttgcaggc gcaaagccgg tactccgtgg atttgtttcg
     2701 catgaagggc gaattgctgc ttgacctggg gcgggaggaa gaagcccagc agttatacga
     2761 atccctgctg agcagtcggg caattccgtg ggcacggctt ggcctggcca aggccatgcg
     2821 caattcgggc aatctcgacc ttgcggcaga acagctgcag gtcgtcatcg aggaaatgcc
     2881 ggaatacctc gcggcctacg atgtgctggc caaggtgcac gaagaaaacg ggcatcccga
     2941 actggcgcag gaatcgctgg ccaatgccct gcgcatgtcg cccggcaacc tggtgcggca
     3001 gagcgcactg ggccaggtgg cgtttcgtaa cggcgatctc gaaaccgcca cgcgagcatt
     3061 ttcgtcggtg gtggacaaag gcaggtattc gttttcgcgt tcgcacaacg atttcgtcaa
     3121 tctgagccgg gtgcagatgg agaatggcga gttcgccaag gcgctgcaga gcctgaatga
     3181 cgccagaagt gcgtttggca cagcgccagt ggttgaactg gccacttcga cgatggaaac
     3241 cctgattcac catgccgcca ataatgctgc cgcatccacg gctgcgcttg acaaggcaat
     3301 tgccgccagg gcagaagccg gcatcgacct ggccggacag actgccattg atctggccaa
     3361 aggttgcttc gtcaatggac gtgtggacga agggcggcaa ctggtgcgtg acatggtaag
     3421 caactattat gacgacacca tggtgaaacg atcggtgcgc cagatgctcg atgccgttgg
     3481 gcgtggcgat gaaagcaagg cactgatcga agacagtgtt gcagatgtca tcaagctcaa
     3541 caacgatggg gtgttgcttg cgcgtcaggg caatctggcc ggggcaatcg atttgttgct
     3601 tgaggccgcg cgccggatgc cgcagaatgt ccagcttggg ctcaacgcag cccaggcatt
     3661 gatcgtggaa agcgatcgta ctggctggaa cgaggaacac atgcgcgagg cacaacgtct
     3721 gcttggcagg cacgcggcag cgcatggcga cttgccgaag ttcaaaaaga tcaagggcta
     3781 cctgaaagat gtcgggttga aatttggggt aaaggtgaca tgaatccagc acgcgaaaac
     3841 gaaatcggcc tcaaggacgt gctggcggcc aacctgcacg aaaccaagaa cctgatgggg
     3901 cagctcatgc tgcggctcga tgccatgggc acctccgagc cgccggtgcg cgaggcgcgt
     3961 tttctgtgcc agcagatcag tgaccgcatg gtgcagatgc tgctgttgtt cgaattgcag
     4021 gacgagcagc tcaagtctca gcctgaagct cacaatccgg ctgatttttt gctcgaattg
     4081 cgagaaaatg cacgtgcctg ggccggcgca caactggtga tcgaaagtcg tgacgaaggc
     4141 gcgccggact attggtattt cgaccgtgca ttgacggaga tggctctgat gaatgcgctg
     4201 cacaacgcgt tccgccatgc ccggcagcgt atcgaactgg tcaccggcat gcgcggcgac
     4261 tgtctgtatt tttccgtgcg tgacgatggc gccggttttc cggacagcgt gttgcatgtt
     4321 gccggtggac acagtctgcc gatatcccgt gacggtaccg gtctcgggct gtattttgcc
     4381 cgcatgatcg cagccgtgca cacaaatcag gggcgcagcg gctcgcttga gctgcgcaac
     4441 aatgaatccg ggtcgggtgc ggaattcacc ctgttgcttc caaaataaat aaattctgac
     4501 attgacatta tgtatctgca cgaatatcag gcaaaacgca tcctgcgcga acacggaatg
     4561 aacacgccgc gtggggtggt ggtggaagat gccgctcacg ccgaacttgc agccaccggt
     4621 tatctgggtg gcgagtcatg ggtggtcaag gcacaaattc attccggagg gcggggcaag
     4681 gctggcggtg tgtgcaaggt cgattcactc gacgcgctgc gcgaaaccgc cgccggcatg
     4741 tttggatcgc gactggttac gctgcagaat gcgccggacg ggcagccggt cgaacgcttg
     4801 ctgatcgagg aaaccctggt catcgagcgc gaactgtacc tgagcctgct gcttgaccgt
     4861 gaggccgaat gcctgacgct ggtggcctcg gctgccggtg gcatggacat cgaagccatc
     4921 ggagcgcaga cgccggaacg tatcctgcgc gaacgctgcg atccggtcat ggggctgcag
     4981 ccgttccagt gtcgcgcggt cgcgttcggt ctggaattgc caaccgccct gcatgccgat
     5041 tttgttcgca tgctgcaggg actgtaccgc gtcgcgactg aaatggacct ggatttgctg
     5101 gaaatcaatc cgctggtgat cactcgggac ggccggttgg tcgcactcga ctgcaagatg
     5161 gtggtcgatg acaatgcctt gccgcgccag cccgcactgg atgaattgcg tgatgacaac
     5221 caacgcgatg cgcgcgaggt ggaggccgtg catgccgggc tcaattacat cgcgctggat
     5281 ggcgatatcg gctgtctggt caacggtgcc ggcctggcca tggcgaccat ggatttgatc
     5341 cgcctgcatg gtggacgccc ggccaatttt ctcgatgttg gcggcggagc caccagccag
     5401 accgtggcgc gagcgttttc catcttgctg gccgatccgc gtgtcaaggc ggtgctggtg
     5461 aatatttttg gcggtatcat gcgctgcgac atcatcgcag atggaatcat tgccgcgatg
     5521 cgtgaaatcg gcgtcaaggt gccggtggtc gtgcggctgg aaggaaccaa tgtggaactg
     5581 ggcagggaaa aacttgcgca aagtgggttg acggtgctgg ctgcgcagga tctgaccgat
     5641 gccgcacagc gtgcagtggc agcggcggag gccgcaaaat gagcatattg gtacaggcag
     5701 atacccgcgt gctctgtcag ggctttaccg gcaagcaggg gacgtttcat tcggaacagg
     5761 cgcttgccta tggcacgcgc atggtgggcg gcgtgacgcc tggaaaaggc ggtcagcagc
     5821 accttggcct gccggtgttc aataccatgc gcgaagccgt gcgcgaaacg ggcgccacgg
     5881 ccagcgtgat ctatgtgccg gcgggttttg ccgccgattc gatcatggaa gcagccgatg
     5941 ccggcatcga gctgatcgtg tgtatcaccg aggggatacc ggtgcgtgac atggtcaacg
     6001 tcaaggccgc gctgcgctcc agtaccgcca aattgatcgg acccaactgt ccgggtgtga
     6061 tcactccggg cgcatgcaag attggcatca tgccggggtc gattcatcag ccgggcaagg
     6121 ttggcattgt atcgcgctcg ggtacgctga cctacgaggc ggtgcatcag accacacgca
     6181 tcggtctggg tcagagcacc tgcgtcggca tcggcggcga tccggtcaag ggcatggatt
     6241 tcgtcgactg cctgcgtctg tttgaagctg acccgcaaac cgaggccatc atcctggtgg
     6301 gggaaatcgg cggcgcgcgt gaagaagaag ctgctgaatt catccgcact tcggtcagca
     6361 agccggtggc cgcctatatt gccgggcaga gcgcgcctcc gggaaaacgc atggggcatg
     6421 ccggggcgat cattgcttcg ggtgcaggta ccgccgaagc caaaatgcgc gcgctgcgcg
     6481 atgcaggcgt ggtggtggcg caatcgcctg ccgacatggg gcgtgccgtg atgcaggcca
     6541 tgcagcaatg aaaatcgccc tggcgcagat caatgccact gtcggtgaca ttgcaggcaa
     6601 caccgcccag gtgatcgatg ctgcccgtca ggcacgcggt cgcggcgcga acatcctgct
     6661 gacgccggaa ctggtggttt cgggctatcc ggccgaagat ttgtggttgc gcgaggacct
     6721 gcacaggcgt gtggaacagg cgctggttga attgtgcaca cattcgcatg gcatcacgct
     6781 ggtgattggc cacccgcacc gcgtgggcaa agaggtgttc aatgctgcgt ccgtgctgcg
     6841 cgatggcgcg gtgctggcga cctatcacaa gttcagtctg cccaaccaca cggtattcga
     6901 tgagcagcgc tatttcgtgg ctggggaacg accgtgcgta ttcgaagcgg cgggcatccg
     6961 tttcggcgtg accatttgcg ccgatgtctg gcatgaaccg gcggcgcgca gcgcagccga
     7021 tgccggagca caggtgttgc tggtgctcaa tgcctcgcca taccatttcg agaagcaggg
     7081 tgagcgctac gacgtcgtgc gcgatcgtat taaggagacc ggcctgccag tggtgtattg
     7141 caatctggta ggggggcagg acgaactggt gttcgatggg gcatcgttcg ccatgaatgc
     7201 gcaaggtgag gtgaccagcc agttcccgcc attcgaaacc ggagtgtatg acgtggaatt
     7261 cgacggcacc cggctgcaag cggggccggt ggccgaaccg ttgcccgccg aagccatgtt
     7321 gtggaaggcg cttggacttg gcctgcgcga ctacgtgacc aagaacagtt ttcctggcgt
     7381 gctgctcggg ctgtccggcg gtatcgattc ggcgctggtg ctggcgctgg cggtggatgt
     7441 gcttggggca gggcatgtgc acgcggtcat gatgccatca ccctataccg ctgacatcag
     7501 cctggatgat gcgcgtacaa tggcgaacgg gttcggcgtg aaatacaccg aattgccgat
     7561 cggcggattg ttttccgctt ttctgcatga gctggcaccg gtattttccg ggctagagcc
     7621 ggatgtcacc gaagagaaca tccaggcgcg catccgtggc accttgctga tggccctgtc
     7681 caacaagtcg ggcgacctgt tgctggccac gggaaacaag tctgaaatgg ccgtgggtta
     7741 tgctacgctt tacggtgata tggccggagg ttttgcgccg ttgcgagacg tgagcaagac
     7801 ctgggtctat cgcctgagcc gctggcgcaa tagcgtcgct ccggcgattc ctcagcgcgt
     7861 catcgagcgg ccgccttcgg cagaattgcg cccgggacag acggatcagg atacgctgcc
     7921 ggattatggc atgctggatg ccatcatcga gcgttatgtg gaatgggatc agagcgtgga
     7981 agccatggtt gctgccgggc tacgcgaggc ggaggtgcgc cgtgtggtgc gcatgatcga
     8041 ccgcagtgaa tacaagcggc gtcaggccgc tcccggcgtt cgcatcactg aacgggcatt
     8101 cggcaaagac cggcgatacc cgataaccca gcgctttgtt ccctgagggg aagtgtggag
     8161 gaagtggcat gaaaaaaatt gacgcgatca tcaagacatt caaactggac gatgtgcgtt
     8221 cggcattgtc ggaaatcggc attaccggca tgaccgtgac cgaggtcaaa gggttcggtc
     8281 ggcagaaagg tcataccgaa atgtatcgcg gtgctgagta tatggtggat tttctgccca
     8341 agctcaagat tgaggtcgtg gtgccggacc atctgcttga cgatgcggaa gaagcgatca
     8401 ttcgcgctgc ccgtaccggc aagatcggcg atggcaagat ctttgtcacc aatgtcgagc
     8461 aggtggtgcg catccgcact ggagagcgcg gggaaaccgc aatctgaact tcgatcccag
     8521 gcgcttcctg gcctgatctt cctggcctga tcttcctggc ccccgaatgg agacaggtta
     8581 cagatcgggg tcccgcacag ggaatccgag ctcgcgcagc atgtgcagcg tcgggttcag
     8641 gctcctgagg acgaactgct tgcgtaccgg attgcgccgt gccatctcgc gccactgctt
     8701 gcccggcgtc agcccggcca tctcgtacac atggcttggc ggcagataga acgtccgcgt
     8761 gaactgggca atcagcaggt tgagcaccgg tgtgagcaga aggcgcttga aacgtgaggt
     8821 gttgcgcaac ccggcttcga gcgccgcgcg cgcatgcgcg atgtggcgcg cctcatcgat
     8881 gatgtgcagg gtgcacatct ggtggatgac gccgtccact accttgccac cgtaagtgcg
     8941 gatatggcgg ttgagccggt cggggatttc ttcgccgatg gttaccgcaa gccagaacag
     9001 cgaactgccc agcggcgtgt ggcgaccgag aaaatcggtc agcaacggac ggttgcgcca
     9061 ggtgcctggc aggtggatgc cgctggtttc catcagcttc aggaacatca ggctgtgacc
     9121 ggattcctcg cggatttcat gcagatagta acccagttcc tgcgcggaca gcgtatggcg
     9181 caggttcctg gccaaccgtt cgatgaaaat accctccagc cagatgccgg catcgacgaa
     9241 ggcgacgaat tcgagctgcg acaaccggat gcgcgatgct tccggcaggc tgacgaattc
     9301 agcgaggccg gacagcgaga cagcatcctc cggcagccag ggccggttca tgtccagcgc
     9361 gctccagtcc agcttgagca gcggatcgat gtacggcagg ctgttggtgg aaagctgttc
     9421 cagcagggag atttctgcgt gcgccatggg ttgcgggtga caaaatctgc acaagcgccc
     9481 tatgatacca aatttgtccg ttaattacag cagccgcgtg ccttgcctgg aaacacgtta
     9541 tcgggtatgc tagcgcgctt tcctgctgtt gccctgcacg gggcagcgcc tgcgggtcaa
     9601 agtccggtca cgccgcgcga tccggagaga tggatgagtg gtttaagtcg cacgcctgga
     9661 aagcgtgttc aggataatat cctgacgggg gttcgaatcc ccctctctcc gccaataacc
     9721 tgctgattta attatattct gttttggatt tctgcccaaa aacaggcgct gatcaacctg
     9781 tcttcggcag cttttcgtcc gctttttttc tgaacatttc taccgctttg ccaccgacct
     9841 ctggtgccgc atctgcaatc caggcgatct ggctgactat gcacacggca tgtgctcggc
     9901 tgccttttac aaaaggcgga gtaagtttgg cggcatggat gcttctctga tgccccggct
     9961 caaggagctt gaagatgaga accaacggca gcgcagcgta tttgcctggt gttgggcagc
    10021 gcggactcgg acccaaacca gccaaattcg ccgacgaaca tcccgtcttt aaaatcaatc
    10081 gtcagccagt ttggcacaaa cgcttcgctt ctggctacgg taaagtccgt taccggaggc
    10141 ggccccatat agtccgagac caggcagttt ttggtcatga caatattgaa tcgcacgtgg
    10201 atcttgccgg ttaagggttg ggcgtcagcg agaatttccc ggaatgggtc ggcttccagt
    10261 cggtatgtgc caggaatgtc ggcagcgcta tagtaggcca gcctttttcc tatcatgcag
    10321 ctaaagtaga tctgcatttc catgaccact ggcgcatcga gcctgtttgc catcttttct
    10381 gccttttcac tgcattcgat tttcagcgct ttgccatgaa atgacaggtt tcttgtcatg
    10441 ctgatctcct gatcgctgtg tgtacacccg cgttgtgttg cgctgcatgc ctggttcgga
    10501 caccagtgct gcatcagaca ggcttgtcgc accagtttac cacccggatc gttcggtggg
    10561 gatggcgcta tcgttcggtc agaaccgctc ccgggcatgt tcgatccagt catgcagggt
    10621 ctggagggcc ggttgcgctg gctggtcgat ttcgggccac tgccgcaagg tctcgcccag
    10681 gatgttccgc acctgccagc gtgcttcgcg tatcagcaga ttgagttcga gcaggcagtc
    10741 gcgatcggtt tcgatgtcgt gggtcatccg atgcgggctg gtgggatcga aaccggcgtc
    10801 gaacaggttc tttttcagga tgtcgagacg gtcggcaacg taccgcatcg ggcgaggagc
    10861 atgctgccgc agatgggggc cgaccattgc gtcccatgcg gcaagcaaac cggcaatgtc
    10921 gtccaggctg gcgcgcaatg cgtcaagatc cttgatgtcc tcagcgaatt gctgagtggc
    10981 gttcggggtg cgtggggcga gaagatactg tgcaagatcg ttgcggaatt ggccgggatt
    11041 gaatttgaat gccatgttca gccgttcgga actttgatcg tggctgccgg tgcacaggct
    11101 gcctgtcact tcggcgacgg cgagaatctg tgccagcgga tgaatgtcgt gggcagtcag
    11161 gtggcgcggg tagccgctgc catccagccg ttcgtggtga tcgagcacga tttcgctgat
    11221 gtcggggtgg tactccgggt attcgcgcag cagcagatag gaaatgaccg ggtgggcata
    11281 gaactgtttg tcgagcactt cggggttgat gtgcatttcc cccaggtcat gaaaaatggc
    11341 cgcgctggcc agtttgtgct ggttgtcggc actgaggcgg atctggctgg caaggtagag
    11401 actggcggcc gcgacgcgca ggctgtgctg gtagatcacg ggccattttt cacgggcgag
    11461 cgtcagtttg aacgccaggg cggaattgag cacgatgcct gacaggcgtt gttgtatgac
    11521 tgtcaggtcg cccaggccgc tacggatgcg ttgcatcaat ctgtcatcac tgtcgaacag
    11581 ttgttcggtg tccagccaga ggtcgcgggc agtgagcgcg ccttcgacgc tgaggttttt
    11641 gtcgatgggc gcggcggcca gtttgtgccg catcagtttg tcatagaaat tgcggttgat
    11701 gggcgtgcct tgcgccacca gtttgatgcc cgtgctggag tagatgtcct cgctgcagac
    11761 gactttccgg acgtcgctga attcagtgac attcctgatg taatacggat caggattggg
    11821 aaggtcgtcg ttaacctgga tcatggcacc cgggtgcagg atgagtcccg gttacggtac
    11881 gccagacgtg gtgtagcgtc tcttgtgccg gggagttgtt ccggctcgcg tacccgaccc
    11941 gggatatgtc aatcagccac caaagcggtt tgtgatgacc acctcgtcga tgggcaactg
    12001 cccgccgcgt ggccaggctt cgcgcgtggc gcggccaatg gcaagcatca tggcaatgac
    12061 atggtcgtcg ggcaggttga tcagcttgcc tacggcatcg aaatcgaaac cgtccatggg
    12121 gcatgagtcg taacccattt cgcgcgcctg cagcatgagc gacatggcgg cgatgccgca
    12181 ggaccgcatg gcctcgtcac gctggacttg ctcgcgaccg tcgtaatatt ggtggatggc
    12241 cggcaccatg aaatcgctca cggccgcgtc ggcattgcgc cagtatcgtt cgggctgttt
    12301 gttccatgcc ttgaggtcgg cacacagcac gagcagcagc gaagcgtcgg tgacctgggc
    12361 ctggtcccag gccacggcac ggatggcctg gcgcagggcc ggctcgcgca cctgcacgaa
    12421 gcgccagttc tggatgttga acgcagtcgg tgacagcatg gcctgcgaca gcagatggcg
    12481 gatttccgtt tcgctcatgg catgctgcgg gtcatattgc ttgacggatc ggcgggtgac
    12541 aatggcttcg gataccttca tgaaatgctc cggtcaggta gaatggcgct cacgtgcgca
    12601 tgaatgattt tggtagtaat gttacggtaa agctggtgga tcgtccatac gattgcccga
    12661 ttgtggcagt gcggatattt acgactcaat gttccggggg gaagaagcca tgcaggtggt
    12721 gacaacagaa aacatcgccg gataccgggt ggtgcgcgtc attggtcagg tgtacggcgt
    12781 gaccgtgcgc tcgcggggat tgggaggcaa cctgatggct tcgctacgct ccatccttgg
    12841 cggggagatc gtggaataca cgctcatgct ggaacaggcg cggcgccatg ccatcgaccg
    12901 cttggtgaag aatgcgacga tgatgggcgc caacgcggta gtcatgatgc gcttcgattc
    12961 gagtgagatc ggccagatga tgagcgagat cgttgcctac ggcacggccg tggtggcaga
    13021 gcagatcgaa ccatgagaac tgcagtgctg gtcgtgctgg gcgtattcac ggcaatcggt
    13081 gccgtgctgg tggcgatggg cgtgttgtcc gctttgcata tgctgttgat tccgggattg
    13141 ctgttgctgg ccgtgctgta tgagcgtcat gggtatgtgc ctcgcgctgg cggcacgcgt
    13201 gcgctggaag acacgggaga ggtgttcgaa gatccggtca gtggcaagcg cgtgcatgtc
    13261 cgcatcgatc cgcagactgg cgaacgtcag tatgacgatg aagcatgacc catcaggcag
    13321 ggctgctgcc tgagaccgtg ctctgccact gactattggc aaggatgcaa ggtttcaaca
    13381 caatacaact gacgtcagca ggcatgccaa taatgaggcg gatggaaggg gcggagtgga
    13441 caacggtgcg aatgccagtg cgcggattca gctggggaag aacgggcggg cggtactgca
    13501 tgcagacgag acgctggcgc tcctgagcgg atttgcggtg cgccagtttg tggacgaagc
    13561 gcccgctttc ctgtcacgaa ttcatgctgc agaccgcaat gtgctgcaga catggatgca
    13621 ggcgggggcg gaagggccgt tgccgctgcg gcacctgcgc atgtttcatg cggatggcca
    13681 tgtcgtgtgt ctgcaggtgc gtgcagtgcc aactgcatct gctgcaggcc tcgtggttga
    13741 tctgttcctt caggatgtgc gtaccctgca gaccgatgct gcggcctggg gctggctcgc
    13801 atgcctcgcc ggctggctgc gccagacatc cgacgccgtg tgcattgcca atgcggatta
    13861 cgttcgcctt gcggccagcg actcgatgga ggcgctggtc cgtcaggccg gtttcgatgg
    13921 taccttgcct ggcaagacgc tctacgatgt cttgccgcgg gcgtatgcag accggtgtca
    13981 tgcactggag cagcgcattc acgccgggga gctctcggtg cgcgccatcc tgccgctgca
    14041 tcggcccgag ggcggtcgta tctggctgga actccggatg tatccgctgc acaacccggc
    14101 cggtgtgctg aacggtgtgc tgggcatcgc acacgatgta agcgattgcg cgcgcaatcg
    14161 cgcggaattg cagcgcaatg cccgccgttt gcgccatctg gccgatttca ataccttgct
    14221 gtcgcaggtc aacccagcca tcaacagcgg taaggatgag gcacaggttt ttcaggatct
    14281 gtgtgcgctt gcgctgcgtg atgtgcagtt gtatctggga ttctatgccg tgcggcccga
    14341 tcagcccgaa ccggtttgtc ttggcggatt gccgccggaa tgtggcggga acatggcgca
    14401 atccgaaccc gtgccgcgac ttgccctgga gctgatcgag cgtgcatggc aggaagagac
    14461 tccgcagttc gacgccccgt tgtcgtctgg cgaggatggg cagcgcacgt tggcacaacg
    14521 cgctgcgcag gctgcgatcc cattgtttcg ccaggggcag atacacgcgg tgatgctgat
    14581 ctgccatcga caggccgatt tttttgaccg ggacatccgc ctgttgctgc atgaactggc
    14641 gctggatatt tcacgcggtc tcgaccggct tgattcgcag cggctggaac atgccctgct
    14701 caatcatgcg cagattggtg tgttactggt gcgtaaccgg gtgatcgagt acgccaatcc
    14761 ctggatcgaa cgcatgtttg gttatgcgcc ggcggaactg gtcggccacg atacggaagc
    14821 gttctatctc gacccccggc aatatctcga tgtgggccat gcgtaccatg agctgctgct
    14881 gcacggggcg gtgcatgtgg ataacctgtt gtttcgcagg cacgacggca ctgccatgca
    14941 ttgcgacggc accgccgtgc tgttgtccga tgcgcaggac ggccgggtgc tgtggacgct
    15001 gcaggatgtc acgcagcgtg aaacccaggc cgtcagcctc agacgcattg cccggctcaa
    15061 tggctggctg gccgacatca atcgcatgca tttcaccatg gacgattcgg cgatgctgtt
    15121 cgatgcggta tgtaaatccg cgctgcagca tcaggtggcc gctggaatat gggtgggccg
    15181 gccggcgctg gaagatgggg tgtttcacgt gcttgccagc gtagacctga cacatggcgc
    15241 cggctggctg gaccatcacg accggccaat cagtccgctg gctgcgcagg cgctgcacga
    15301 gcggcggctg gtactggatg cgtccgaaca gctggcgcca gtcatgcggc atgaactggc
    15361 tgcattgccg atcgtcgagc atgaacgtgt catcgccgtg ctggtgatct gcggtgacgg
    15421 tgatgaaatc agcgcagcgg attctctcga actgcttact gcgatccgca ccagcatcga
    15481 acgcacgctg gtggacttct ggcagcgtgc gcgtattgcc agtttgcagc ggctgcatca
    15541 ggcgttgatg gccatgggtg aagtcgtgct gacttcaccg gatggcgggc acatgttgca
    15601 ggggacttgc gatcagcttg tcaaccagac actgtttcac acggcctgga ttggccgccc
    15661 cggtgctgat ggcatcattg aggtactggc gcacgcgggg gattcgatgg ggatgctgca
    15721 cgagattcgc gtcaccatca tgaatagcga atgcattttt cccgtggtgc gtgcctggaa
    15781 cgaacagcac gtcgaattgc gcaatgacct gcttggggat ctgcagcatg cgccgtggca
    15841 tggtttttat gccaagcacc gttggcatgc cctgcttgcc gtgcccatcg tgcgttcggg
    15901 ggcgatatgg gcggtgctgg tgttcgtgtc gtcgcggcgc aacgtgttcg atgcgcagac
    15961 gatcgaactg tgtggccggg tgaccggact gctcggccat gggctcgacg aactcgacct
    16021 gaagcagcgc ctgagcgata tgcagcgcga cgaactgtat cgcgccgagc atgatccgct
    16081 gacggacctt cccaatcgtc gccagttgaa ccagtatctg cctgccgcga tgtcgcgcgc
    16141 acgcaggaac ggttattcac tggctgtggg cctgatcgat ctcgatgact tcaagccagt
    16201 caatgacagc tgggggcatg aagccggaga ccggttgctg aagattctca gtacacgcat
    16261 gcgcgatcag ttgcgcgaga cggatttcct ggcacggctg ggtggggacg agttcatcat
    16321 cgtcattgag gggctggacg aattcgaggc ggccagccag ctggaagtgg cgttgcgtcg
    16381 tctgcaccag gcggtcgaac aggcgttcga aatcgcaccc gggcagtcag cgtgggtcgg
    16441 catgaccatg gggctggcgc tgtttccgcg cgatgccgat gatgccgatg cgctgattcg
    16501 tctggctgac gcggccatgt atcaggcgaa gcagatcaag gaaaaccgta cccagtggtg
    16561 gcggctcggc gtcggcccgc tggagaccat gcgcagcgaa ccggaaatcg acagttacgg
    16621 tgcggaggcc accgtgctgc tcgaacgcat gcaaggacat attgcggccg tggtggagca
    16681 ttttgtccat actttttatg gtgaactggc gcagagcacc aatccgcagg aaattcttgc
    16741 cacgctgacc gacgaagaac tgcacatgct gatgcaacgt caggccgagc acctgcgctt
    16801 cctgttggcg ccacagacga cccgcgaggc catcatctcg cgtgcgcgcc gggtcggtga
    16861 ggtgcatgcg ctttccggtg tgagtggcgc gctgctcatc cgttcgcaga cactgtatca
    16921 ccgcacgctc aaccgtcacc tgaaccgcat gccgatgcgt tcgtccgacc ggtaccggct
    16981 gatggtgatt gccgaatcgc gcctgcagga cgatgtgcag gttgaactgg aagctggcgg
    17041 caaggtcatg cgcacctggt tcgatattct gtcccagccg attccgcccc atggcacact
    17101 gtggatggat gcggtgagca gcgagatcgg gcggctgggc acgctgcccg gcttgcgggg
    17161 cgcactgttg ctgcgactca ataccgaggg tgtgtttact gttgaaagac atgccggacc
    17221 ggttgccgat ggcatcgtgg cgatcatcca gcagcctggt cacgaggtgg tggtcgaccc
    17281 gacatccgag cgcggccaga gcctgacggc ccaggcatgg cgtagcctga aaattctcag
    17341 tacgccgtcg cgcaccaagg atccgaatta ccgtttctgg cgggattctt cgccagacct
    17401 tggggtgcgt tcggccatga gtctgccgat tcttaacggt gaagggcagg tgcaggctgt
    17461 ggttgcgctc tatggcgcca tgtcgaacca gttcgagtca gccgagatgc atcagtgggc
    17521 gcggggcttg cagcaacgct gggaacagct gtgggtccgt tgcagcactc cggcgccggt
    17581 catttcgcag gaacgggcga ttctgttgcg gcatacgttg tttgcaggcg gattgcgcat
    17641 gtacatgcag cccatcatcg acctgcacag cggtcaggtg gtcaaggtcg aggcgctggc
    17701 ccggctggtg cagccggacg gcgaggtggt cacgccggca atgttcctgc cgctgctggg
    17761 agattccgat ctggaccggc tgttccgcgc cgggctggat ctgacccttg gccagttggt
    17821 gcaatgggag gcgcaaggac tgtcggtcga tgtctccgtc aatctgtccc ccaattcgct
    17881 gcttgatccc gattgcagta cttgggtgcg tgaggcgctg cagcgttatg gggtggcacc
    17941 gcagcgtctg acgctggaac tgctggagac gcaggaactg gattacaccc ggcaggatgc
    18001 ggccatcaac gagctggtcg gactcggggt caagctggcg atggacgacc tggggtcggg
    18061 ctacagcagt ctcaagcgtc tggctgcgtt gccattcgac accatcaaga tcgatcagga
    18121 cctgctggtg cgcatacgcg aaaatccgct gcaaacgctg ggcttgattg gcgccattat
    18181 ccagatggga cgtgattttg gccgcaaggt ggttgtggaa ggcatcgatg atgcgggcgt
    18241 gatcgaggcc atgctgacgc ttggcgcgga ttacggacag gggtacgggc tgggcaagcc
    18301 gatgccgggc gatcagctgg tgtcctggaa cgggttgttc catcgcacct ggcagcctgc
    18361 cgctgaattg cgtacgtttc tcggcgcact gtcgtggcgc tggctgtccg ggcacagcgg
    18421 ggtgtgtgcg catccggtgc aatatggtga ttgccagttg acggcgtttc tgcaacagca
    18481 tgccggggcc ggctcggacc cggcactctg gcacgagcgg gtgcatgcgg atccgggtgc
    18541 cgggcaggca tcgcagcaat tgttcgactg gctggtgcaa caggtcaaag tgcgcatgta
    18601 ggcgcacatc gcgctggtca tcctgccctt attcccgcgc tcccagttca atcttgctgt
    18661 cagccatttc gctgtcgcgg tcgttggcct ggggcgcggc gatatggatg cgtgccgctt
    18721 cgactttgtc gacctgttcc agttgtgtgc ggatggcgtc gccacgttgc tgcgccagtt
    18781 gctgcaactg cgtctggttg accggaatgg cgtcgcgcag gcgctggatc acggtttcat
    18841 acagttgcca ttcgtgcgtt acatgggttt cgctgcgcaa tgccttgatg ccgcccagcg
    18901 cagggaattg cttctcgtag cgtgcggcga gcaccttctg ggtacgccgg tcggttgggt
    18961 cgagcgggcc agggtctgtg ccgggggtga aatgcagccc gattgcgctg gcgacctgct
    19021 gacgcagttg caaccggcgt agcgcctgtg catcgccgct cgattgccag ccgggcgtga
    19081 tcgtcagtgt gagcgcgggc cgcttggcca gcgcttgtgc cagatgctgc agcttttcct
    19141 gttccggtgg cggcagtgcc gcctgaccaa acgggaattt gagcgcttcc atctggctgg
    19201 tctcgatgcc cagcaggttg gccagcgcat ggaacggcgc ggtcacgatt ttggtcagca
    19261 cgttgaccac ggctttccag atgatcctgc catagctgaa ttgcgggtcg tccaggttgc
    19321 cggaaaccgg caggtcgagc tggatgacac cgttgctgtc ttcaagcagg gcaatggcca
    19381 ggttgagcgg caggttgaat gcatccttgc tgtccacgcg ttcgccaagt ttgagctggt
    19441 gaatgatgaa ggtgttgttg ccggtgagct ggcggttgtg tacggtgtat tccaggtcgg
    19501 ccgacatgcg gcccgagtcg atcttgcgcc cggcgaactt gccggaatac ggcgttagcc
    19561 gcgccatttc caggttgcgg aaatgcaggt ggaggtcggt gtggtcgctg gcatgaaacg
    19621 gctggatcga tccgcgcagg ctgacggaac cgtactggtc gacctggccg tcgatctgca
    19681 ccagcgcaat ggcgttctgg cgcgaggaca gacccatgac cacgccgttg aggtcgtgca
    19741 tgcgcgtgcc aaagggcgtc ggcaacgaca ggtcggcata ttcggcactg ccgcgtgtga
    19801 tgcgcaggcg cttgacgccg atgtcgaagg cggagttcgc cttgtggccg gcagcggtcg
    19861 gagctgtgga tgtcgcatgt gcgggcgacg gatcggttgt cgggtcagct gccgccagcg
    19921 gcgcccgagc ggcggcagac gcggccgcag gtgccggcgc atgccagtcg cggcggatgc
    19981 ggtcgaggtt gatgctgtga tccgcaaaaa tgatgatctt gacgaatggc tggttcgcat
    20041 ccagttcggg gatgcgcaac cggtcaggag caaggctgaa atccagcgtg tgcgcagaca
    20101 gccggttcca gccgaggaag tcgttgccgg tatcttcttc cttgattgac aggtggtcga
    20161 tggcgacgct gccatgatag cgtagcggaa actggttgtg ggtacgggcg aaatgcagcg
    20221 taccctgggt ggtcatgttg ccgtcatgca gcctgagccg ggcgacttcg ttgagatagg
    20281 ggccgaatgg cagtaacgac agcgcgcgca actggacatc gaactgcccg gaagttgcgc
    20341 cgggagccaa ctggccatta atgtgaaaat ggcccccctg gcgcacgtcg aaggcgatat
    20401 cgagaccgat cggctggtcg tctgccagtt tcaggccgtc agcgcggatc tgtccgtggt
    20461 cgatgtcgaa ttgtacggca tgatgcggca cgtggtcgct ggcggcaagt tgcagctggt
    20521 cgagctgcag gtgcgccagg cgcacgtccc aggggggggg cacggtcgcg tggctgttcg
    20581 gcaccggcat cgtggcggcg gccgttgcgg cggcggcagg tttcggcgct gggtgcgcca
    20641 gcagttccga ccagctgagc tgtttgtcgt gccggcgcac cagctgggag cgcagcccgg
    20701 aaatgtgcag ggtcgtcagc cacaaccggt gctgcgccag gtcgaagctg ccgtcgtcga
    20761 gccggatcga ttgcagttgg gcgagcggtt gcgactgcga gccagcgaac agcgcaaccg
    20821 cagacgattc cgcgtgcagc ggtgcgattt gcagcgcaaa gtgcggtgtc agtgtcatgt
    20881 gcaggccaaa acgtgtcgtc agcccgtgca gttcgagccg caacggctgc gcgagccgat
    20941 catcgtgcaa tgtgacatcg gtctgttgca gctggacgag accgtccgtc agctcgaggc
    21001 tggatgggtt gccgccggca aggcgcaggc ggtagggcag tctgctgtct accgtggcgt
    21061 gcgcgagctg caacggcaac cggattccgg tcatggtgat gagctgcggc acattcagtc
    21121 catcgagcgc aacggtgcca tcactttgca gcggatcgat gagcagcgtg ccgttccagc
    21181 cgatgcgcgt gccaagcgcg tcgatgcgcg tggtcaggtt gaagaccgcg gggtgggtgg
    21241 acagtgtgct cagtgcgcgc agttcaaagt agaggggttg caggtgcaga tcgagcggtg
    21301 tcggcgtcag ggtggcatcg tacatggcca gattgccgct gcggatggcg agccggtcga
    21361 tccgcaggcg tggcatcgga ctgggggcag gattgcggtg tttgtggacg gccaccagca
    21421 gttcgtccca gtcggtccgg ccatcgcggt tgcgtctgaa ctcgaccgtg ggagcatcga
    21481 gactggcgtc gcgtatatgc cagatgaagt gcagcaacag gtcgcggcgg tcgatcacga
    21541 catggatctt gcggacgctg gcgagtggcg caccgtcgtt acgcgccagc gtcagtccgt
    21601 cgacggtagc ctcaagggta atggggttga agcgcacatg ctgcacttgt agccggctgt
    21661 cgaactggtg ttcgccgatc cagggcaaca gtgatctggt cagcggattg atcagatagt
    21721 aaccggtaat caggtagagg gtgcacagtg cgatcagcag aaacagcacg atacgcagcg
    21781 cgcgcaacag ggatggtggg cgaaaatggg tttgcatggg cgcggctccc tgtcttgacg
    21841 cctgtgaggc gttcttttac ctgaacctga tgaaggtccg gattcattct accgcgttga
    21901 aacgggggat gcgagtgttg ttcagcggtt gcatccgcgc cgtggggcag cggatgcctt
    21961 tcctgcggca agtgacttac tgcgctgccg cgggtgcaga cgcattcccg gcgcgagcgc
    22021 gcatgtggat gcggcgccaa ccggccatgt catcaaaggt cttgcgttgt tctgcggtca
    22081 gcagatcgta gaggcgctgc gcagcagcgg ctacgtccga catgctgtgc gcatgctgtt
    22141 gcatcatttc ggcggcgtgg gccatgcgct gcggtgcggg agcttcgtcc ggggcgtttt
    22201 ccatggcttt gcgctcatcc tgcatcagat gcgcctgggc catcaccttg tcggcaaagg
    22261 tttgccaggc cggttgctgt gcgtcggtga ggctgagcgc tgccttgagc cgtgtgagac
    22321 ggtgctgggc aatggcgacc ggatcgggca tgcgacgcat catggggcgt gctgcggttg
    22381 atggcgctgc tgaatttgcc gggggcggcg tgggggaacc gtctgccagg gcgacgccgg
    22441 ccgacagcgc ggccagtgtc agggcaatct gcatggactg gagtttcatg aaatttcctt
    22501 tcagcgcaag tgtggcgatt gaaatgaacc gggtgcaacc gccggttgga atcaggtgtg
    22561 gtgctttggt tcccgcagtg ttggccgact cgccggtatc gggcgatgcg tgcgtactat
    22621 acggcctcga tcagcccatg ccatggcagg gtcagcagtt ctgtcgggtg gctggtttgc
    22681 aggatggcct gccagtcgtg cagttgctcc ggtgaggtgc gcagcagcag gacatagtgc
    22741 ccggcctcga tcgcggcgtg gaggtgatcc agatgttcgg gcgggatgcc catgcggtgc
    22801 agtacgatcg acaggtggct gatttcggcg gcgccagcca tggcagcacc gcccagcgct
    22861 ccgccaatca gcgtgccggc tgcttccgaa gcaagtacgg ctgcaagcgc ctcggcgatt
    22921 gaaccgccgg cgatgatcgg gccgatgcct ggcagtaaca cgaacatggt ggcgccggca
    22981 agcatgcccc agacggcacc ccagaatgcg ccctgttttg cccatacccg catgcgttcg
    23041 ccggggccat ggtaggagat gccgagcacg tcgtcaccgg ccggatgcac cttgcccagt
    23101 acggaaagca tgtccatggg agcatctttt tcctgcaata cgtgcaccgc ctgctgtgcg
    23161 tggatttcgt cgtcgaatac ggcaaccagc agtacggtgt cgccagacag ggcgtgtgaa
    23221 gattcggaca tgggcctctc ctcgaagggg gtggtttaca gccaaatgaa agggaaattg
    23281 ttggattttg taagcaatag tcaaaatcct gctacaggac tattatagag ccttatgcta
    23341 ctgtcttgtg tagtgcggta acgctcgccg cttgccgtga accgtcgggg gtggcatgaa
    23401 acaaacggga ggcgcgcagg gcgtgctgca ccgtgttgaa cgatgatcgg tgtgtgacat
    23461 gatccggatg cgacgcaaag attaccggct tcggcgggac cgcagggtat cgctgtccat
    23521 tcatgcgttt ttcgtgctga ttgtgatcgt gctgctgacc gtgtttggcc gcagtctgga
    23581 gcagttgcat gacgccatgc tgcagcgcag ccagcaacgt gcacacaacg cggttgccag
    23641 cgttgcgcag gaaatcaggc atgccatgga tcgccgctac gatgaaatgc gcttcctgcg
    23701 cgaagccgtg ctgcttgcgg ccggggggcg ctgggtgagc gatccggctg ccgcaaggct
    23761 ggtcgagcgc ttccgtctgt cacacgctgg tgcgctgaca gtctggatgc tcgacccgtc
    23821 cggaaagacg ctgctgtgtt cgtcccgtca accctgtccg gccacgccgc ccagtgtcag
    23881 tgcatatgca tccggcccgc caggcaacag caccgccatg cccgtgtcga ccccgggtgc
    23941 gcaggactgg gtgttcccca tggggctgga cgtcatgggc gggcagggtg tcacgggtcg
    24001 actggtggtg gtgtggaaca tcatcgtaca gcatgaactc gacaagcctg aagcggcccg
    24061 ggtcgagttg ctggataccg ccagcggtca ggtcatctgg cgcgatcatg ccgggggagc
    24121 caccgcggct gcgctgaccg gcgtgccggg gcgtgcagcc aggccggcgc aggccagcca
    24181 tacggtgccg ggttttccgt ggacggtgcg cgtgcgctgg caggacaccg tggcacagaa
    24241 ggcttttgct gccgaaatgc gcttccgtat tgtctggctg gtatgggtgc tggtgctggt
    24301 ggcgctggtc gacatgctgg tgcaatggtt cgtgcgtcgt gtcaaccgtt tgcgtgaata
    24361 tcaggccgct gcactgcgta ttcagcagga actgcttgcg ctggaagatg aacagcagat
    24421 tttccagcgc gtcaccgatc tggttgtgag tgagaccgag gcgattgccg cctatgtcgt
    24481 ggtgcgtcag cccgacaacg accggttgct gccggtcgcc gccagcgctg accaggaagc
    24541 gttgcgcgaa gcgctgatgc aactgatgcc atcgctcgat ccggcacttc agccgcaggg
    24601 ggggctgctg ccaagcatcg ccatccgtga acggcgcgtc gttggcccac aggacccgcg
    24661 cgcggtggca gccatgcgcg ccattcagca agagcacccc gcgttgctgc gggtgcgttc
    24721 ggtcatggtc tatccggtct tcctgccaga gcagactgaa gcgtcgctgg cactggtgat
    24781 cgagagtgcc tctgcccgtc attttaccca ggcggtgcgc cagctgctgc agcaactggc
    24841 actgtcgatc ggcatcacgc tcagccatat ccggcaccat cgcgaaatcg cggaggccaa
    24901 tgccttcaat acctcgttgt tcgataccgt tggcgcgatc atcatggtga tgaacactga
    24961 tggggaaatc gtgcgcatga atggcgcatg cgagcgtttc atgcatatca cgtcggcaga
    25021 ggtggccggc cagccgtatt actggcaacg ttttattccc gaggacgagc gtggtggcgt
    25081 gcgcgcgatg tttgaccaga tcgtcaacca tgccatgccg cgccacatcg agaatcactg
    25141 ggtgacgccg gacgggcaga aacgtctgtt tcactggatc aacaccattg tcgacgatgc
    25201 gcacgggcag gcagaatatc tggtcacagt cggcatggac gtcacggagc agaagaatct
    25261 ggaacaggcg ttgcaggaca atctgcagcg tacgcgtcgc ctgagcgatt tcaatctgct
    25321 gatggcccag gtcaaccatt cgatcgccat ggccagcgac gaagaacaac tgttgcgcga
    25381 catttgcgat ctggcggtgc gtcatgcaca tttctcgctg gcgctgatcg cccggcccgg
    25441 tgaagacaaa cgtttccgtt tcccggcggc cagcggtgaa ttggcctatc tgcaggacct
    25501 gttcatttcc accgaccccg agattgccga aggccgtggt ggttttgcta cggtgtggcg
    25561 ctcgcagcga ccgttgttca atgaccgcgc gctggaagtg gcctatacgt tgccatggcg
    25621 cgagcgtttc cgggccttgg ggtttcattc caacgctgtg ctgccgatca tgcgcaacgg
    25681 cagcatatgg ggtatttttg cgctctatca tgccgaacgg gatgtgttca accaggacct
    25741 gcgcgccatc ctcgaagaac tggcgctgga tatctcgcgc gggcttgatc acctggccac
    25801 gcagaatttg cagcgtgtgc tgctcaacaa ctcggtaaca gccatttttc tggtacgtaa
    25861 ccgtatcatc cagctggcca atgcccgggc cagcgagatg ttcggttttg ctttcggcga
    25921 gatggaaggc atcgaaaccc gacgtctgta tatggacgag gcgcaatggc aggaaaccgg
    25981 cagccagtac gacggcatac tgcgcggcca ggaagtgaaa atcgcttcgg tgtacatgcg
    26041 ccacaagcag ggcaagcagt tgatcacgga catttccgga attctcgtcg atcccgagga
    26101 gaaattgtcg gtgtggacgg tcgaggatgt gacgcagcgc gagttgcagc ggttgcgctt
    26161 gcagcgtctg tcggatttca aggcgctgct ctccgatggc aatgaggcga ttgcgcgtgc
    26221 ggaaagcgaa gcagcgctat tgcagaccat gtgcgatctg gcgattgcgc gtgcgcgcat
    26281 gagactggta tggattggtg cacccgatgg atcgggtgtc tttatctatc tggcggctgc
    26341 cggggctacc gcttacctgg aagggttgca actgtctgcc gatgtcgagg ctgtgacggg
    26401 gagcggtgca gccggccgct gctggcgcag cggtcagccg gtgttcaatc tgttgccagc
    26461 caacgaaccg cagttgcagt cgctggtcgc gcgcatgcgc agttttgata tcggtgccat
    26521 ggccgccgtt cccatcatgc gcagcgatcg tctgtggggg gtcatggcgg tctatcacaa
    26581 cgaggccgac gtgtttgatg ccgaattaca gtccatcctc gtcgatctgg cgcaggacat
    26641 cggactgggt ctggatcgac tcgacagcac cagccgcgaa cgggaagcca atgccttcaa
    26701 catggcgttg ctcgacagca tgacatccgg tattaccgtg gtgcgttatc ctgaacgcat
    26761 cgtcgagcgg gtcaatgcgc gcatgctgga gatgttcggc gcgacaacgg tggagcaggt
    26821 ggtcggtcat tcatccgaac ccctgtttgc cagcaaagcc atgtatcgcc atgtcggcag
    26881 gctggctgcg acggtattgc gggagggaca tggtctgttg cgcgatgtgg cctatcgtcg
    26941 catggacgac agtatcctgt atgtcgacct gtccgggcag atgctgatcg ctgcggatgg
    27001 cgtgcagcgt atcatctgga cattggttga cgtgaccgag cggcgccgtt cggaacagac
    27061 cattcgcgca ctgagcaatt cgcgcgccat cctgctggcg aataccactg ccggcatcga
    27121 cctggtgcgc tatccggacc gtgtgtttgt ggaagtcaat caggggttcc tcaatctgct
    27181 cggttatgag cggccggaag aagtggtcgg ccatggcacc tacgaaattt acgagcagat
    27241 gttcgatgct gagcgtatga aagcgctgtc agaacaggta tttgctgatg gtcaggcgag
    27301 cctgcgcgac ctgcgtgtaa agcgcaagga tggggaggtt cgctatctcg atgtgtccgg
    27361 gcagttgttg cctgacggcg accatggtta tccggtcatc gtgtggacgt cgatcgatgt
    27421 caccgaacgg cgccatttga ccgaagaact ggcacgtcag gcgatgtttg acaaattgac
    27481 cggcttgccc aaccggcgtg cactcgatga agagttcgtc aaagcattgc tgcgcacccg
    27541 gcgggcactg aatctgatgg ccgtggtcat gatggatctg gatggtttca agccggtcaa
    27601 tgatatttac ggacatgaag cgggtgatct cgtgttgcgc acgattggcc agcgtttgca
    27661 ggacattctg cgccgtaccg attttctggc ccggctgggt ggcgacgagt tcgtgctgct
    27721 gatcgaagac tgcgacaatc tcgacgaagt gacgacggtg atggacaagg tatgggatgc
    27781 aattacggca cccatcgatc tgggtaacaa ccaggcagtt tcgattgagg ccagcgccgg
    27841 cgtctgcatc tatccattca gtgatgtcga caatcccgac atgctgatgc gctatgccga
    27901 ccaggcgctg tacgaaaaca agaaacacaa gctggaccgg cagcgtttct ggacagtcta
    27961 tggtgaagcc acaccacagc gtctcaacat gtatcagcgg ctgttacggg aaggcaggct
    28021 ggaagtccat tatcagccgg tgctcgacaa tcacagccgg aggttcatcg gcatcgaagc
    28081 gctggcgcgc ttgcgcaacg acgacaatca tatgctgcat ccgcaggaat tcctgcccgc
    28141 actgtgcagc gatgagctgt ttgacatgag tcgacgcgta ctggatacgg cgttgcgcga
    28201 cttgcggtcg ctggatgccg atgggccggg cggtctgtgg gtgtcggtca atatcgatcc
    28261 gggcaacatt acggcccatt gtgtgcattg tctgcaggaa atcgtggaag cgagtgaaat
    28321 cgatacgcag cgcattcatt tcgaaatctt cgaagggagc catttcgaca cacagcacgc
    28381 cgggatggaa tatctgtacg tgctcagaca gcagggaatt cgttttgtgc tcgacgatgt
    28441 tggcagcgcg tattcctcgc tactgcgact caaagatctg ccgatcgaca agatcaaact
    28501 tgatcaggat ttcgtgcgta cacttgacca gcacccacga gagctgcagt ttgtcgaatc
    28561 catactcgat ctggctaacg gtctcggcct ggagttgatg gtggaggggg tagaaaacga
    28621 tgacatcctc gacgccatga ccgtgctcgg ggtaccgagc ctgcagggct atggcattgc
    28681 ctatccgatg gccttcgccc agttgcgcga atttctgcaa ttgcccttca cccgccatcg
    28741 tcaccacccg gtgagtctgc ttggcgtgta tgccagacag ttggccagcc acagcacgat
    28801 gaggaaagcc attgtgcaga atccgcatct ggtggacgcg aagaaaatta tcgatgccgg
    28861 ccaatgtccg attcatgatg acctggtgcg tctcggcgtt gacgagagcc acgaaatcca
    28921 tcgcttgcat caggcatatc accgcagcat cggagacatg catgcgcagt acattgcgtc
    28981 cggcaacgag cccgactggg ctgcgctcag aaaatcccag cgggcgttgc tcgatgcaat
    29041 catggaggtt tatcagactc gtgccgcaca ggcgcctgga ctcggcgacc ggctgcctct
    29101 gatctgccag taggcgccgg attgcggtgc aggtcctgtc ggtgtcggcc gggatcggca
    29161 gactgggaga aacgtatgga gcatcggcgg cgagccaaag agatcgatct gaccatcggg
    29221 catcacagcg tcaccaagat catcgatcgt cgtgccgatc tgcgtgacct gtatccgatc
    29281 ctgctgcggc tgccctggag ccgattcctg ccgctggtgg tactggccta tctggtggtc
    29341 gacattctgt ttgccctgct gtattactcg gcccctgatg ccatcggcgg catgcatggc
    29401 accgacaggt tcaccagtga ttttttcttc agcgccgaaa ccctgtctac ggtgggttat
    29461 gggcaactgt ttcccgcaac gctgtatggc catatcatcg cgaccattga aagcctgagt
    29521 ggcatgatgt tgatcgcggt cgtcaccggt ctgattttcg tgcgcttttc caagccctgg
    29581 ccgcgggtga tttttgcccg caagatcgtt atcagaccgt tcgatggcaa gccggcgctc
    29641 atggtgcgta tcggcaacga acgcgacagc ctgctgttga atgccgaaat acgcatgacg
    29701 ctgatgctgg aagccgtgac gagcgagggc acccgccact tttccagcta cccgctgcgc
    29761 atgcaggacg atcatgttcc catgtttgcc ctggcctggg tcggcaggca tgaaatttcc
    29821 gcagacagtc cgctgtacgg gctggacaag gcacaaatga aggcgcggcg gatgcgcttg
    29881 gtggtgtccg tgaccgggct ggacgaggtg ctgactgaaa ccgtgtatgt cgcgcaacgt
    29941 tacacgacgg atgatgtgca ttttgatcat cagtatgctg ccatggactc cgatgatcag
    30001 gggcgccatc gcgtcgattt tgcctatttc cacgaaatcg aaccctggca ggggccgatg
    30061 gacgtggggt cagaaccacg ttgaaacagt gtttacgcac accatgttga aacggtgttt
    30121 ccgcacgccg cggtgcctgt tcgtcaggtt ccgggttacg tccaggtggg gctgaaaggt
    30181 ggtttgttga tcaggaggtc ggtaatgtcc ttgctggcca gcggcttgct gaacaggaat
    30241 ccttgcgctt cgttgcaatt gagctggtgc agccattgat actggtcagc tttttccacg
    30301 ccctcggcga tcaggcgcag gtccagactt ttcgccaggg tgcagattgc agtcacgatg
    30361 gattcgttgt ccgagtcgcc ggagaagcgg ggcatgaacg actggtcgat cttgatggtg
    30421 tcgatgggga atttcttgag atagcccagc gaagaataac cggttccgaa atcgtccagt
    30481 gcgattctga tgcccatctc cgacagttgt ttcagctttt caagtatgtg ttcgccatca
    30541 ttgagcaagg tgttttccgt gatttcgatt tcgaatctgg atccgggcaa ttcgtattcc
    30601 tgcaacagat cggcaatgaa tccgaccagt tgggcctgct ccaggtggcg ggatgaaagg
    30661 ttgatcgcga tgcgtggcgg catcagcccc gcttctttcc acagcttcgc ctgcctgaac
    30721 acggatctga tgacgaactc gccgatcgga acgatcaaac cggtttcctc ggccaaggga
    30781 atgaattccg ccggcagcat gtagccccgc agtggcgaca gccagcgtac cagtgcttcc
    30841 atgccggaaa tccgcccggt ggcgagatcg acctgcggct ggtaaaacac ctggaattct
    30901 tccttttcca gcgccttgcg catttgcatt tccagcgaaa accgttcgtc aaagctgacg
    30961 ctcatttccg catcgtagat ctggaactgg ttcctgcctt tggccttgct gtcatacatg
    31021 gcgatatcgg cattcttgat caggacatcc atggagtcgc catgttccgg gtacagggcg
    31081 atgccaatgc tcatcggaac gtgcagctcg tgcccatcga tctggaaggg tgcggcaaat
    31141 gcctggatga tcttgtcggc tgcgcgtatt gcgttttccc gatgggaaat gtgtggcagc
    31201 agcagggtga attcgtcgcc acccagccgt gccagcgtat cgtcttcgcg cagacagcct
    31261 ttgagac
//
LOCUS       DAIANL010000098        17185 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_738049, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000098 DAIANL010000000
VERSION     DAIANL010000098.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 17185)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17185)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17185
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738049"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3513 a         5844 c         4921 g         2907 t
ORIGIN      
        1 ggatctggtc ggcatgcaga tccatggtga gcagtcggtc gatacccgcg ccggtcagca
       61 tgttggcaac cactttggcg gtaatggcaa cacgcgccga acgtggccgg cgatcctggc
      121 gcgcatagcc aaaataggga attgctgccg tgatgcgtcc agcggaagcg cgacgcaggg
      181 catcgacgat caccatgatt tccatcaggc tgtcattggt tggcgcacat gtcgactgca
      241 gcacgaatac atccttgccg cgcacgtgct caagcagctc gaccatgacc tcaccgtcgg
      301 aaaacctggc aaccgtcgcc cgcccgagat gaatattcag gtggcgcacc acatcttccg
      361 ccagccgggg attcgcattc ccggaaaata ccatcaaact atcgaagccc accgctcacc
      421 ccggcaaaaa acaaaagcgt gtaatcaccc attacacgct tcgattcagg aaccacgcac
      481 ttacattgcg ccatcctggc gcacagtatc catggcgacg ccagctcaca ccgggcccac
      541 cgctgcagga ccacaaaccg ttcacggtgt gaccccacac attctggcag gggaggcagg
      601 gatcgaacct gcgaatgtcg gaatcaaaat ccgatgcctt accacttggc gactcccctg
      661 ttgtctttcc aacagctgcc ggtcaaccgg cccaatcata ctgcgggtgc gaacttagtc
      721 cgcgcgccac aaagccgcgc atccctgtcg gcaggccggc aagcacctgt cttgcccgcg
      781 cctcattgtc aaagcaggcg aaaacgcagg ctcccgatcc ggtgatgcgc gccggagcat
      841 actgcgacag ccactgcagc gcttccgcca ccgcaggaaa ttcagcacag gccacctgtt
      901 gcagatcgtt gcgcgtacgc gccatgatcg ctgccaggga caggcactgc ggctgtgccg
      961 acacccctcc ggaaaagtcc ggctgcaccc ctccggaaaa gtccgtaatt ctgacgcttt
     1021 cgctgtttct tgtcaaccgc tggctggcga aaatgcgcgc ggtggaaatt tccactggcg
     1081 gctccagcac cacataccag cttaacggca ggcaaaccgg ctccagttgt tcgccgatgc
     1141 cgcgcgcaaa tgccgaccga ccaaacacga acaccggcac atcggcgccg aggcgcaacg
     1201 ccagcgactg caaacgctgg cgtgaccaat cgagctgcca cagccggttg agtccgagca
     1261 gcaccgccgc ggcatccgag ctgccgccgc ccagtccgcc ccccatcggc aacctcttga
     1321 ccaggccaat atcaacaccg ctggtgctcc cgctggcctg ttgcaacaag cgcgcggcgc
     1381 gcacgcacaa gtcctgttcc gccggcacgc ccggcaggtc gtcattgcgg cagatctgtc
     1441 catcgccgcg cagggcgagg gttaccgtat cgccatggtc aaggaggcaa aacagcgttt
     1501 cgagcaggtg atagccatct tcacgccgtc cggtcacatg caggaacagg ttgagtttgg
     1561 ccggggcgga cacaaccagg cgagcactca cggatcaacc tgccactggc tcaccagcaa
     1621 ccgtgcccgc acgccatcgc ccacaagatc gatgcggtca ggcagacgcc tgtcgcccac
     1681 catgcgccag cgcagatagg tcacggtcca accggattgc tgcatctgca agggggcgtc
     1741 ggcagcatgt cgctgcactt catagggctg ctgcggcact gccgcgccag acacccaatc
     1801 ggccagaccg tcgaacggca acgaccatcc gagtctttgg cgcgacaggc tgtcgagggt
     1861 atgcgcgtaa taggtatgct gtgaactgtc cgtgagcgag gcacccgatg catcacgggt
     1921 caacctgccc agtgtctggc caagtggtga atataattcc accgtatcgc cttgcggcgt
     1981 gtgacgccag tgcagaccca tctgcccgga atccttgccg gcatgcatgg caaggcgccc
     2041 atcaatggaa aacgccggca acggcgcgtc attgagcgcg acactggtca ccggcggcgc
     2101 gggcggctgc atggcgcagc cgcccagcaa ccctgccagc acggtcaaca gtgtcagcaa
     2161 gaacccgcgc agcatcattc gatcacttga acttgtctgc cgccgcacgc aacgcaggat
     2221 tatccggatg cgccggcaac gcagattgcc acaactgacg cgcttcgtcg cgcttaccct
     2281 gcttccacag cacttcgcca agatgcgccg cgatttcggg gtcctgaagt gcgtgataag
     2341 cccgttgcag gtattccgca gcaaggggca gattgccgag ccgatactgc gcccagccca
     2401 tgctgtcaag aatgtaggca tcgtccggat cgagtttgag cgcctgttgc agcaattgca
     2461 cagcctcgtt gaggcggatg ttgtgatcgg cataggtata accgagcgca ttgagcgcct
     2521 gcgcataatc cggcttgatc ttgagcaggt ggcgcaggtc gtgctcaacc acgtcgagct
     2581 tgtcgagcgg ctcggccatc aaggcatggt cgtagagcag atccacggag tcgggtcggg
     2641 tagccagcgc cttgcccagc acgtcatacg cgcctgcatc gtccccagcc tcatgcatca
     2701 gttgcgcctc ggcacgaatc agttgcaccc ggtcatcatc attggtggtg gtcacgcctt
     2761 gcagccagtc acgcgcagcg ggcaacttac cctggtgcgc aagcagcgct gcataacgga
     2821 tctgcgccag gatatactgc ggtcccggtg gcacggcgcg ataccagtcc gccgcctcgt
     2881 gctcatgctg tgtcgcctcg gcaatctgcc cgagatagat gtccagcatg cccggatcat
     2941 ggtaattgag ttccagcgcg cggcggaaag ccgcttccgc acgctggggg tcgttgagct
     3001 gaagcgatac caggcccaca gccacctgca tctcggcgtt ctgcgggagt tgctgcgcca
     3061 gcagggcaaa ctggaccagc gcctcggccg gacggttctg caccagcagc gtgcgccccc
     3121 acgccagccg caaatcggcc gccttcggat gtgcgcccag ataacgcgcg gcgaaggtat
     3181 ccgcctcggc cgggctgtgg tgctccagca cctcgaaccg cagctctgct gccacgtccc
     3241 aatccggctt cagcgcatcg acaccatcaa gttcgtgcaa agcggtgtcg aaatcgccgg
     3301 ctcgcgctgc ggcctgcgcc acagccaggt gagcctccgc aagcttcgga tgttcggcag
     3361 ccaacgtctg caccagcccg aggaccatct tgcggtcggc ctgtccggcc agcaacccac
     3421 ccagacgcat gaattcatta ccggcatgcg ccggatcttt ctgcatggcc tgacgcaggt
     3481 agggcagcgc atctgccggg cggggaccgc caagcagcag tgcaatcacc gcatgcagcg
     3541 cattcatcga atccggatct tcactcagcc agagtcgggc tgcactctgc gctgccgctc
     3601 cctggcgagc atacaacgag gcttcgacag cattgcgcgc caggtcgaca ttgccggttt
     3661 cacgcgccag cttgaggaac atctggctcg ccacgcccca ttggccgcgc tgggcggcca
     3721 cttcagccac gagaaattcg tacaggcgct gcgctgtcag cgcattatcg ccgctgctgg
     3781 ctggctgcgc tgcagccgcc ggaactggaa gcggttgcgc agccggcata gccggttgcg
     3841 cacgcacgga agtcacgcag ccgaccaggc aggcagcgat ggtcagacgg ggaagcaata
     3901 cacgcatcat tctgttcatt ccggagtaat aatggcaact ctaccagagt tggcagctgg
     3961 ttagactgac aacccgatgc aagttccgct gcatcaacgg gaaatctgtt gataacggcg
     4021 ccccgcttcg cgcaagcgcc gccggccgag ctcaaattcc ggctccagcc gtgccagttc
     4081 gtcccagaag cgctcagagt gatcgagatg cgccagatgc gccagctcat gcgccagcac
     4141 ataatcgatc tcggcttcgc tggcctgcac caggcgccag ttgatgcgga tcacgccgtc
     4201 ccgggtacag ctcccccagc gggtacgtgc cgaagtcagc tgcagcgcct gcggctgacg
     4261 tcgtagcgcg ggcaggaaac gctgcatgcg tccggcgaaa catttcgctg cctgcgtgcg
     4321 gtaccaggcc agcacccggt tgcgcacccg cgcaggttcg gtcgcggcga cgcgcagcac
     4381 ctgaccgtca aaaccgacgc cacgcacgcg cgcatccagc gccagtaccg tttcacgccc
     4441 catccagtac agcagcatgc catcgcgcca gtccggtgcc ggcagcaccg gactggcagc
     4501 cagacgggcg cgaatccagt cagctcgcga ctccaccgct gcctcgatgc ggttcagtgg
     4561 caactgccat ggcgcctgca ccaccaactg cgcctcgcgc accaccactg ccagcgtacg
     4621 gcgcgcgctg ctgcgctgca gacgatactc cagttcgtcg ccggcaagct caatacgcct
     4681 cgattccaca ctgcgccgga cggtcatttc actgcggcag acgcagcatc tcggcttcga
     4741 tccactgctg cacctgttcg ttgagcgcat cgggcttcat gccggtcgca tcgatcggcg
     4801 ggccgatact gatcgtgatc atccccgggc gtttgagcag cgcattacgc ggccacaggc
     4861 gaccggcatt gtgcgccacc ggaaccactg gcactttggc atgggtggca agccaggcgc
     4921 caccgatatt gtattttccc gtttcccccg ccgccatgcg cgtcccttca gggaaaatca
     4981 gcacccacat cccctcttcc gtcaaccgct gacgaccctg atcgacgatc tgcttgagcg
     5041 cggcacgtcc ggccgaccgg tcgatggcaa tcggtgccag ttgtgccata ccccaaccga
     5101 aaaacgggat ccgcagcaac tcgcgcttga tgaccatcgc cattggcggc aacagtaccg
     5161 gcaaggcgat ggtttcccag gccgactggt gtttggccat gacaatcgat ggcgtatgcg
     5221 gcaggtgttc ctgtccgata atgcgatagc gtatgccgca gatccagcgc gcaagccaga
     5281 ccacactgcg attccagccg gtgatgacgc gataacgcac cttcagtggg aacggcgctg
     5341 ccagcacgcc aaacagggca aacaccaccg taagcaacac ctgcaacagc aggaacacca
     5401 gcgaacgcaa ccacaccatc aggacaacac cccggccacc gcctgtgcca gatcatcaaa
     5461 cacccgcacc ccctccggca ggttgccttc ggtttcggtg cgcaccccct tgcccgtccg
     5521 caccagcacc ggcaaggctt ctacggccgc agccgcctgc aggtcgcgca agctgtcgcc
     5581 aatggccggc acccccacca gcgaggtatc aaagcgttcg gcgatttcaa gcagcatacc
     5641 cgcctgcggc ttacggcagg cgcattttgc atctgccgca tgcgggcagt agaacaccgc
     5701 gtcgatgcga ccgccggcac cggctaccgc tttgtgcatg cgggcatgaa tcgcattgag
     5761 catggccatg tcaaacagtc cgcgcccgac gccagactgg ttggtggcca caaccacccg
     5821 gtaaccggcc cggttgagcg tggcgattgc ctccagactt cccggaatcg gtttccattc
     5881 gtcggggctt ttgatgtaat gatcggagtc ctggttgatg acaccgtcac ggtccagaat
     5941 gatgagcttc atcgcgacag tataacccgg attgttcagc caattgtaac gacagggaca
     6001 aaatcacgac ggccgttcga tggcgaccag ccgtgccgca tggatgcggg cccccgcttc
     6061 acgcgggtca cccgccgcgg ccagttcggc aagcggtacc gcccgcgccc gcgccagcgt
     6121 cgtgcgcagc agttcggcct gcgtaaaggc atcatgctca tgcccgggac gtccacaggc
     6181 atccgccttg caggccagca gcacatgttc gaaccgttct ggccggcgaa aggcgtcaag
     6241 ccgttccagc aggcgcagcc gggtctgtgc gtcagcacgg cgcgcaccat gcacctcgcc
     6301 atgacagcgc gtcaccagca gcgccatgtc gcggcattcg ccaggaacac gcagacggtc
     6361 gcacagcgcc cttaccggca cttcaccgcg catctcgtgc tgcgggtgct ccggcagcat
     6421 ttccgctggt gtcagcgcct tgccggcatc atgcaccagc gcggcaaagc gcaccgccag
     6481 cggcgcctgc atgcgcgccg cgctgtcgag ggccagcaag ctgtgcacac cggcatcgat
     6541 ttccgggtgg taagccgggt tctgtgccac gccgaacagc gcatcgattt ccggcatcag
     6601 ccgcgcaagc gcaccacatt cgcgcagcac ggcaatcatg cgcgacgggc gctcggtcat
     6661 cagcccgcgg gcaaactcct gccacacccg ctccgcgacc agatgatcga cttcaccgcc
     6721 atccaccatg cgccgcatca accccagagt ctgcggcgca atcgaaaatt gtgtaaaacg
     6781 cgccatgaaa cgtgccacgc gcaggatacg caccggatct tcgacgaacg ccggccccac
     6841 atgacggaat atgcgtgcag ccagatcggc ttccccgccc cacggatcga tgagacgacc
     6901 atccgcagcc cgcgccatgg cgttcacggt caggtcgcgc cgcgccagat cctcttcgag
     6961 cgttaccgcc ggatcggcat gcaccacgaa gccccgatat ccgctgccgg atttgcgctc
     7021 ggtgcgcgcc agcgcgtatt cctcatgggt ccgcggatga aggaacaccg gaaaatcccg
     7081 tccgaccggc acgaatccct gatccagcat ctgctgcacc gtggcgccga ccacgacgaa
     7141 atcacggtca gttaccggca agccgagcaa ctcatcgcga actgcaccgc cgaccacata
     7201 agtttccata tcaatccgcc tacccgtcgt accgcggatt gtaacagccc aacgcaagcg
     7261 gaagaaaccg gaccggcgcg ccacagcccc cgcatgcact gcgccatacc acgcagtgca
     7321 tgtcgtgaag cgtccagcag gaatgatcag gcgggatggg acgagtagaa acaatctgaa
     7381 ggcaatggct gcccgagtcc gatctgctga tcggaaaact gcgccagccg tgcctgcgcc
     7441 gcacgcgcgc aaccgccatt ggcatcaagg ctgggcatgt cgccggcgaa gacgcgattg
     7501 atgtccgcaa agcgcgtccc ggtttcggcg atgaaataat caaagcgacg cggcacgcga
     7561 cgatctgcgc tcatgaccgc gcggtacagc cactgcggca ggcgcaagcc acgcagcaac
     7621 cggtcggcat gcgccagcat gcgcaccgag cgcctggcct gatcggattc gagcaaaaag
     7681 tgacgactta ccaaccgcac gatgaccgcc tcgccctcgt gcagcagtcc ggccggggta
     7741 tcggcgacct gcgggcgcac atgtgcgtcc atcgaccatt ctgaaatggt atgcgccacc
     7801 atcggccagt ccacccacag acgctcgtgt gcagggacga aatgatggtg cgccaccaca
     7861 tcggcgaaca aatgcgaata gaaaccgacc accagcgcac gctcctcatc gtcctgcgca
     7921 cctgcaagca tctgtcgcgc attttcccag cagtgggtgc gatcgaatgc ccgggtaccg
     7981 gccaaccggc ccacgagcgc cagatccgga aggcaggccc ctgccagcac cagtctggga
     8041 tggcgggtca cggcgcggcg caattccccg tcaagcaggg gaacgcccca gatcagccac
     8101 tgggcaaaga acacatgggt atacaggccc caggcatgcg cgctgaccga atgtaacagc
     8161 cacggcaggc accacaacag cacgcgaacg ggttttttca tcctgtcgat ctcccacgtg
     8221 aacccgggac gcgtaaatct gctttgtctg cggccagcca tgctgctggc acccaacggt
     8281 cagacgacgt cctgtgttgc gggcgagaat acccctgaca caagacagat tgatgatctt
     8341 caggtgaaat tttgatgacg accggcgcgg cagacgccac gccggttcga gcaaccggtt
     8401 cgggaaaccg gctcagtgat cgccgccgcc ccagtcacga cgaccgccat cgcccggatg
     8461 gtcattacca cgaccatcac cgcgattctg ccactgtcca tcgggccgcc cccgcccgta
     8521 cggcggccga ccgtcatgac ggtggtattc acggtaacgc ggcgcgtatt cattgcggta
     8581 ccagtcatcg cgcacgaaat acaccggtac cccgcaggca tggtactggc tgcaatagcg
     8641 gttccagtgc cgttcatggc cgggcgggac gcgcagatag accggcggcg gaagtggtcc
     8701 gtacccccgc tgaatcagga tcggtgccga atagatgagc ggaggcgggg cgaaaccgcc
     8761 cagatcgagc cgcccgtaaa aatgcggatc accaatggca atcgatacgc cgacatccgc
     8821 tgccgccggc acggccggca acatcgccag cgcgagactc atgcataaca aggtacgctt
     8881 catgtctgaa cactcctcag gaccattgca caatcaagcc tccattacga cagcataatg
     8941 tcagcatagt ttcatccggc acgcgcggtc gcgtgtgtgg caaacgatcg gcccgcgctg
     9001 ctctggtata atgccgcatt gaccagctca atggattcta caatgtccgt actgatttgc
     9061 ggttcgctcg ctttcgattc gatcatggta ttccaggatc atttccgtca ccatatcctg
     9121 ccggaaaaaa tccacatgct gaacgtgtcc tttctggtgc ctgaactgcg ccgcgaattt
     9181 ggcggctgcg cgggcaacat cgcctggaac atgcgactgc tcggtgacga accgctcatc
     9241 atggccacct gcggcaacga tttcccgcct tatgccgaac acctcgcccg acacggcatt
     9301 acgcagcagc acatcctgcc ggtcgacggc agttataccg cccaggcctt catcaccacc
     9361 gacctggccg acaaccagat caccgccttt catccgggcg caatgaactt ctcgcacagg
     9421 aaccatgttc cgacaggggc aggcatccgc ctgggcattg tcgccccgga cggtcgggag
     9481 ggcatgatgc agcacgcgca cgaattccat gccgcaggca ttccgttcat tttcgatccc
     9541 ggacagggaa tgccgatgtt cagcggggaa gaattgctct ccttcattga actggccgat
     9601 tacgtcaccg tcaatgacta cgaagcggaa atgctgcagg cgcgtaccgg gctgacgctg
     9661 tcgcaactgg cagcgcatgt gcgcgcgcta atcgtcacca agggcgaggc aggttcgcac
     9721 attcatgccg acagtaccgt gtacgacatt ccctgtgcgc ctgttgcaca ggcgctcgac
     9781 ccgaccggct gcggcgatgc ctatcgcgcc ggcctgatcc atggcatcct gcacgacctt
     9841 ggctgggaac gtaccggtcg gcttgcgtcg ctgatggggg caatcaagat cgccagccgc
     9901 ggtggacaga accatgcccc gacgcgcacc gccatcgacg cgcactatct cgaggccttc
     9961 ggcaccccac tgtacggctg acacgttccg cgccgggcaa catcatcgcc gcccggcgca
    10021 gctccgcagc accgacttgt caaaccggcc ggcaacaccg acaatctcca tgtcctgttc
    10081 cggagcgaag acctatcatg cgagccattc caatgatcgc gtttctttcg tgcacactgc
    10141 tgttgccgct ggccctgacc ggctgcgcca gcgggcctgc caacggcgaa gcccgctatg
    10201 ttgaggaagt ggtgccaggt gtcgtcactg aagtgctcac tgtcacgcca ggcacaaacg
    10261 ccggtgcgac gccaccgaca ttgcagatca ccatccggct gaattcagac cgtatgatcg
    10321 ccgtgaccga gaccaccgcc gagccgttca ggaccggcga ccgggtacag gtgctcagcg
    10381 gcaacggaat aacccgcgtc acccactgac gcgggccgca cctgcggcgc acacggtgcg
    10441 tcaggcggca ttacgtccgc tggacgacag attcaggtca agctgcaagg tatccagggt
    10501 actcgatgcc ctgccgccat tacgtgcggc ctcgatctga tccagatacg cctctgtgac
    10561 atcgccagtc acatagaccc cgttgaaaca ggacgtatcg aaatgggtca gcaacgggcg
    10621 taattcgcgt actgcaccga tcagcgcgtc gagatcctga tagatcaggg catcggcacc
    10681 gatttcgtgc gcgatctcga catcagtacg gccggtagcc agcagttcct gacgggtggg
    10741 catgtcgata ccatacacgt tggggaaacg caccggtggt gccgccgagg cgaagaatac
    10801 ccggtgcgcg cccgcatcgc gcgccatctg gacgatctcg cggctggtgg tgccgcgtac
    10861 gatggagtcg tccaccagca gcacgttctt gttgcggaac tccatgccga tggcattgag
    10921 tttctgtcgc accgatttct tgcgcatggc ctggcccggc atgatgaacg tccggccaat
    10981 gtaacggttc ttgatgaatc cctcgcgata gggcagaccc agcgcgttcg ccagttgcat
    11041 cgcactgggc cggctggtat cgggaatcgg gatcaccgca tcgatcggca cgtccgccca
    11101 ttcgcgccgg attttttccg ccagccgctc ccccatgcgc aagcgtgtct catataccga
    11161 gacaccgtcg atcactgaat ccggccgtgc cagataaacg tattcgaaaa tacagggtgc
    11221 gtacttcggc tgttcggaac actgacggct gtgcatacgg ccatcccggt cgatgaagac
    11281 agcttcgccc ggcatcacat cgcgcacaac gcggaaatcg agcacatcga gcgcaacgct
    11341 ctccgacgcc accatgtatt cagtacccag ctccgtctgg cgctccccga tgacaagcgg
    11401 gcggatgcca tacggatcac ggaatgccag cagaccgtga ccggcgatca tcgccaccac
    11461 ggcataggcg ccacggcaac ggcgatgcac gccagccacc gcggcaaaga tccgctccgc
    11521 ctcaagttgc tgacggccat ccactgccgc cagttcgtgc gccaggacgt tgagcagcac
    11581 ctcggaatcc gatgcggtgt tcacgtggcg cagatccagc cggtacaggt cttccttgag
    11641 ctgatcggta ttgatcaggt tgccgttgtg cgccagcacg atgccaaatg gcgaattgac
    11701 gtagaacggc tgtgcttcgg cactcgacga tgcattgccg gcagtaggat atcgcacgtg
    11761 gccgatgccg gaattgccca acaggttacg catatcgcgc gtgcgaaaca cgtcgcgcac
    11821 caggccgttg gccttgtgca tgtgaaacac attgccttcg gcggtcacga tgccagccgc
    11881 atcctggccg cgatgctgga gcaccagcaa cccgtcgtag agcagctgat tgaccgggga
    11941 atgcgagacg attccgatga ttccgcacat atcaagattc ccgataatgt aaaggttttc
    12001 ccagtccgcc tggcgccagc gcatgcaccg ccaacgccat gcggacaaaa tacggcgcaa
    12061 gcagcgactg cttccaccac gcggtttgcg gcaacgccgt ccagcccgcc agccacacca
    12121 gcgccagcac aatcagcaca ccacgcaaca ggcctgtcac tgcacccagc gcgcgattca
    12181 tgatacccag cccaagtgcc tgaaccgccg cagagagcac caggcccacc aacgacatga
    12241 cgagcagcgt tgcaaggaac accagcgcga acgccacatc atgtcttagc gcaccaccgg
    12301 aaatctctgc tggcagccat acctgcaccg gcgcagcaaa tgccttggcg acaaaccagg
    12361 ccacgaacca ggacaacacg gccacaattt cgcgcacggc accccggacg aacccaagca
    12421 gcgccgacag cgccatcacc gcaacaatta cccagtccag cgcgccaatc gtcatcctgc
    12481 ctccgccggt caatgtccgg gcagcacaag ctgcgccggc agatccgcca gtctaagcgt
    12541 cgccagcgca tgttcagccc cggcccggtt cgcataaggt cctacccgca cccgcgtggc
    12601 cccggacggc atggtttcgg tatagcacgg catgccgatc gcctgtaaac gtttgcgtag
    12661 ctgctgtaca tgctcctgct tgccaaacac gccgagttgc acggcatact gcgtcttgcc
    12721 ggcatgggca gccgacgtgt tcgctgacgc agcatgcgag acagcgtcgt gtgcaacctt
    12781 cttgttcctg tcaggcgccg gggatgtcgt tttggctgca tgcgacgcaa caacagcctc
    12841 ttcggctttg gcccgagcgg cggccatgtt ttccgccgcg tgcccatccg cgatcgcgac
    12901 ctgcgcttta ccgctcgccg gcgtggcttg cgcaacggtt ccggccggtg cctgcggcaa
    12961 tgcggacaca ggcacagact catgcgcggc gaggtcgcgc gaagacgaaa caacaggggg
    13021 tgcagtgtcg tgcccggacg gaagcattgt cgacggcacc accgacactg ctaccgcgtc
    13081 ggagcccgcg ctcgttaccg gcaacgctcg cttcatgctt ctgggcgcgc tgtccagcac
    13141 ccagggcacg agccacgcca ccagcgcaag cagcaccacc gctccggaca actgccggcg
    13201 tgcccggtgc ctgcgctgca actgctcaac cgatgacatc tgcgtggcca tattcacccc
    13261 ggctgaccgg cgatcgcccg caacagcggc gcgaccaact gaaacgaacc aaaggcgacg
    13321 attctatcat tttccccggc ctgttcacag gccatctgcc aggccccgac caccgaactg
    13381 caaacgcgcg cgtgggtcac acccgcctgc cgcagctgct gcaacagcac agcgggctcg
    13441 agggcacgtg caccttcgag cggaacgacc agccattcat ccacttcgtc gcgcacggct
    13501 tcgatcacac cggcgatatc cttgtcgccc aacacggcaa atacggcata cgtgcggccg
    13561 ctgacacagg catgatggcg cagattgtcc gccagtgccg cagcgccatg cggattatgc
    13621 gccacatcga gaatccgcaa gggaagcccg ggcaaacgct ggaaacgtcc cggcagcacg
    13681 gcctgcccca atccgcgaac gatcgccgcg ctgtccacgg gcagcagcgg atgcatcgcc
    13741 tcgagcgccg ccagcgcgca ggcggcatta tcgatctgca tgttgccgct catcgccggt
    13801 aacggcaagt ccagctgcca gtggcgaccc gcataatgcc agccatgctc atcacgtgtc
    13861 acgacatagg cttcgcccag tgcacgccag tccgcgccga tggccgttgc aaattcccgc
    13921 aaggtgaccg gtggccgagc atcaccgcag actgccggcc gtccgcgacg atagatcccc
    13981 gccttctcaa aaccgatcgc ctcacgcgta tcacccagat agtcggtgtg gtcgagcgca
    14041 atgctggtca cagcagcgca atcggcatca aacacgttga cagcatcgag tcgtccgccc
    14101 agccccactt cgagaaccac cacatcgaga cgttcgcgta cgaacagcca catcgcagcc
    14161 agcgtaccga attcaaaata agtcagggaa atcccgccgc gcgccgcctc gaccgcagca
    14221 aacgcctcga ccagggcggc atcactggca tccacaccct gcatgcgcac ccgctcgttg
    14281 taacgcacca gatgcggaga ggtatacaac ccgacgcggt agcccgcagc acgcaacaca
    14341 ctgtccagac aggtacatac cgaccctttg ccattggtgc cgcctacggt gaagatgcga
    14401 aatggcaagg tgtgcggcaa caatgcacgc gcaacctggc ctacccgttc cagcccaaga
    14461 tcgatggcgg tcggatgtcg cgtttcgagc cggtgtagcc agtcgtccag ggtcatccgc
    14521 tgaccagcgg gcgctgttgt agcatggaca gcatcccgga caaccgctcg cgcatctcgc
    14581 gccggtcaat gatgacatcc accgcgccgt gttcctggag aaattccgaa cgctggaacc
    14641 cttccggcaa cgtctcgcgc acggtctgct cgatgacgcg cggaccggca aaaccgatca
    14701 gcgcccgcgg ctcggcaacg atcacatcgc ccagcatggc aaaactggcc gacaccccac
    14761 ccatggtcgg gtcggtcagc acggaaataa acggcaaccg ctcgccggac aaccgcgtca
    14821 gcgcggcact ggtcttcgcc atctgcatca gcgagaacag cccttcctgc atgcgtgcgc
    14881 cgccactggc ggcaaagcag acgaatggca gatgttcacg cacgcagacg tcaaccgccc
    14941 gcacaaagcg ttcgccgacc acagcgccca tcgaaccgcc catgaaacgg aattcaaacg
    15001 ccgctgccac cagccccacg tcgcacatcg ttccgcgcat gacaatcagt gcgtcgcttt
    15061 caccggtatc gcgctgcgct tcgatcagac ggtcggtata gcgtttggtg tccttgaact
    15121 tcagcgtatc gactggcgtg acgtcgttgc agatttccac ccgccccgac tcatcgagca
    15181 acattgacag gcgcttgcgc gcgcctatgc gatggtggtg gccgcacttc ggacagacat
    15241 gcaggttgcg ttcgagatcg gcgcagtaga gcacggtctc gcacgactgg catttgctcc
    15301 acagcccttc cggcacggct tttttcgggt catccgccac cctgcggttg atcttcgggc
    15361 gcaggatttt ctggaaccag ctcatttcat agtccttttc ggcatggctc aggcatcgag
    15421 cgcagcgcgc acgctggcca gccatgcagt cagattgtgc agcaatcggt cgggggggga
    15481 ttgctcgatt tcttccacga gacgactgcc gatgaccacc gcatcggcaa acttgccgat
    15541 cgccgcggcc gtttgcgcat cacggatgcc gaaacccacg cccaccggca agtcgcactg
    15601 gcggcgaatc agcggcacgc gacgcgccac ttcgtccaga tccagtgcat tcgatccggt
    15661 cacccccttg agtgaaacgt aatacacaaa accgctggcc tgccccgcga ccagcgccat
    15721 gcgggcctcg gtcgtcgtgg gcgccagcag gaaaatgtta tcgaggccct gatcggccag
    15781 cacctgacgc acactttctt ccggcggcac atcaaccagc agcaccccat cgacgcctgc
    15841 ggccacagca gcggcggcaa acctggcata gcccatgcgt tcgaccggat tggcataccc
    15901 catgagcaca accggtgtct gcgcgtcatc gcgtcggaac tgtgcaacca gttcgagtac
    15961 ccggtgtacg ctcatgccct gtgccagcgc acgctccgag gcgcgctgga tggccggccc
    16021 gtcggccatg ggatcggaaa acggcacgcc cagctcaaga atatcggctc ccgcagccac
    16081 cagtgcatgc atgatggcta ccgtcacctg tgggctcggg tcgcctgctg tcacatatgg
    16141 aatcagcgcc acacgcttgc cggcccgcaa acgggaaaaa gtctgttcga tccgcttcat
    16201 ggatggttcc ccgtctccag gccaatgcca gccaaccgcg ctacggtatt gatgtccttg
    16261 tcgccccggc cggacaggtt gaccagcagt atgcggtccg tcgacagctg aggggcaagt
    16321 tgtgcggcgt aggcaagcgc atggctcgat tcaagcgcgg ggatgatgcc ctcgtagcgg
    16381 cacagatcgt gaaatgcctg caaggcttca tcgtcggtga cagacacgta ttcggcacgg
    16441 cccgtgtcct tgagccaggc gtgttcagga ccgacacccg gataatcaag gccggcagac
    16501 accgaatgcg tctcgatgac ctgaccattt tcgtcctgca tcaggtaact gcgaaagccg
    16561 tgcagtacac cgggagtgcc tgcagaaagc ggtgcagcgt gacggccgct tgccagccct
    16621 tcacccccgg cctcaacacc gatcagccgc acggcttcgt gttcaagata cggatggaaa
    16681 atgccgatgg cattcgatcc gccaccgacg caggcgatga tggcatccgg ctggcggcct
    16741 gccagctccg gcatctgctg cagacattcc tcaccgatca ctgactgaaa atcgcgcacc
    16801 agcatcgggt acggatgcgg gccggcggca gtacccagaa tatagaaggt cgaggcgacg
    16861 ttggtcaccc agtcacgcat ggcctcgttg agcgcgtcct tgagcgtacg cgaaccggaa
    16921 tgcacgggca ccacggtcgc gccaagcagt ttcatgcggt agacgttggg agactggcgc
    16981 gccacatcct cagcgcccat gtagaccacg cattccaggc caaaacgtgc cgccaccgtt
    17041 gcggtggcca cgccatgctg accggctccg gtttcggcga tgacgcggcg cttgcccatg
    17101 cgccgtgcca gcagcgcctg cccgatggta ttgttgatct tgtgcgcgcc ggtatgattg
    17161 aggtcttcac gcttgagata gatct
//
LOCUS       DAIANL010000099         5771 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1358180, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000099 DAIANL010000000
VERSION     DAIANL010000099.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358180"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1054 a         1612 c         1911 g         1194 t
ORIGIN      
        1 gggacctctc atgacgcttt gtgatcaatc gctgccgaac gttcgcgcca tttcgccgta
       61 tcttcccggc aagcccatca cgcaattgtc gcgggagttg ggcattcctg cggaacacat
      121 catcaagctg gcgtccaacg agaatccgct gggcgcaagt ccgatggcgc tcgatgccgt
      181 cgccgaggcg atggctgatc ttgcgcgcta tccggatggc aatggacatg agctgaaaac
      241 tgcgctgacg gtccgtctgg gggtggatgc gacgcagatc gtgctcggta atggttcgaa
      301 tgacgtgctg gaaatggcgg cacgggcatt tttgtacgca ggcacttctg cggtctattc
      361 tcgttatgcc tttgcggtct atccgcttgt ggttcaggcg tgcggtgcga ccggcatcga
      421 agtcgctgcg cgggatttcg gtcatgacct ggaagcgatg ctggcggcca tacggccaga
      481 cacacgcatg gtgttcatcg ccaatccgaa caatccgacc ggcacctttc tgtctgcaga
      541 cgtgttgcgc ggttttcttg aacgcgtacc gcgccatgtg ctggtggtgc ttgacgaggc
      601 ctatgacgaa tatctgcccg atgaccagcg ttctccctcg gtatcgtggc tgggcgtgtt
      661 ccccaacctg atcgtgacgc gcacgttttc caaggcgtat ggtctggcgg gtctgcgcgc
      721 aggtttcgcc cttgcggacg cacaggtggc cggattgctc gaccgggtgc gccaaccgtt
      781 caatgtcaac agcctggcgc tggttggcgc aacggcagcg ctgagcgata ccgggttcgt
      841 cgaacgcact cgggcactga accggctggg catgggccag ctggttcagg ggctgaagtt
      901 gctgggggtc gatttcatcc cttcccacgg caattttctt gcctgccgca tcggcaatgc
      961 caccgcgatg taccggcgtc tgcttgagca aggggtcatc gtgcgtccgc ttggcgctta
     1021 cggcatggac cagtatttgc gcgtcagtat cggactggcc gcggaaaatc accaattttt
     1081 gcaggcgctc gaaagcgcca tggaggaacg gaaatgatca ttgtcatgag cccgggggca
     1141 agcgaacagc agatcgaggc agtcgtgaag aaaatcaagg agtacggttg cgaggccaat
     1201 gtgtcgcgtg gtgttgaacg caccatcatc ggagcgatcg gcgacgagcg tgccatcaac
     1261 caggaactga tagatgccat ggccggagtg gaccagtcga tgcacatcgt caagccgtac
     1321 aagatcgttg cccgtgaatg gcacaaggaa aacagtgtgg tcgatgtctc cggcgtgccg
     1381 gttggcggca agaccgtgca ggtcattgcc ggcccgtgct cggtggaaac ccagccacag
     1441 atggaccagg cagcgcaatt cgtgcgcgag gccggatgtc gcctgatgcg tggcggggca
     1501 ttcaagccgc gcaccagccc atattcgttt cagggcaagg gcgtggaagg actggaaatg
     1561 ttccgccgtg ctgcggatgc gcagggcttg cccattgtga ccgaactcat ggacatccgc
     1621 atgctcgata ccttccttga atggaatgtg gatgtcatcc agatcggaac gcgcaacatg
     1681 cagaatttcg acctgctcaa ggaagtgggg cgggtcaaca agccggtgat cctcaaacgc
     1741 ggcatgtcag cgaccatctc cgagtggctg atggcggccg aatacattgc tgcgggcggc
     1801 aatcacaaca tcattttctg cgagcgcggc atccgcactt tcgagacggc ctaccgcaat
     1861 gtgctcgatg tcacggccat tccggtactg aagcgtgaaa cccacctgcc ggtgatcgtc
     1921 gatccgtcgc atgccggtgg caaggcatgg atggtgggcg ccctggcgcg tgcggccgtg
     1981 gccgctggcg cggacggact gctggtcgag atgcatccga caccatgtga ggcctggtgc
     2041 gatgccgacc aggcactggc tcccgacgaa ctcaaggtac tgatgggcga gttgggcggg
     2101 attgcacgcg tgatcggtcg cgacatctga cgccgatggc cgaacagggc ggttttgtac
     2161 cgatccgccg cctgaccgtt ttcggagttg gtctcattgg cggttcgctc acgctggcgc
     2221 tcaaagccgc cggagcggtt gtcgaggtga ctggtgtcgg acgcagcctg tcgagcctga
     2281 aactcgcgcg cgagcacggg ttgatcgacc ggatgacgga caatgccgcc gaggccctgg
     2341 aaggggccga agtggtcatg ctggcgatgc ccgtggggca gatggacagc gtaatgcgag
     2401 ccattgcgcc acatttgcgt ccaggcatgc ttgtcaccga tgcaggcagc accaagcagg
     2461 atgtggtgtc actgatgcgg cagcatctcg gagcgcacct gcgtgattgt gtgccgggtc
     2521 atccggtggc cggagccgag ttgagcgggc cgcttgccgc gcgcgctgac ctgtatcagc
     2581 gccgcaacgt ggtgctgaca ccgctggcag agagcgatcc ggccggcgtg cagcgtattg
     2641 ccgccatgtg gcgacagtgc ggtgcacagg tgcgcagcat gacggcggca gagcatgacg
     2701 ccatttttgc cgcagtgagc catttgccgc atctgctggc gtatgcattg gtagacatga
     2761 tcgccgcgcg cgacaatgcc ggacaactgt tcgattttgc agcgagcggt tttcgtgatt
     2821 tcacccgcat tgccggcagc agcccggaga tgtggcgtga tattgcgttg gcaaaccgag
     2881 aggcgctgct ggcggaactg gatgcttatc tggcgcaggt gggccagttg cgtgccgcgc
     2941 tgcaacgtgg tgatggcgct gcgctggagg cgacgtttgc tcaggcccgc tcggcgcgtc
     3001 aggactggct cgaccggctg cgctgagtca gcaccggctg cagctcatga tgccatcctt
     3061 acgccaacga tgaaataggg ctcgaccggc tgcgctgagt cagcactctg gccgcagctg
     3121 tgcgtgctca atggccgccg ttgtcgtaga aaaactcctt gatcaggaag tcttccagcc
     3181 ccgcattgtc ttccgggcgg gcggacaacg ttatggtctg gcccagacgc tgtgattccc
     3241 attcgaaagt gtcggggtgg cgcttgcgaa tgatggcgat cattttgcgc acgatggcat
     3301 gttcgatgtc gctgtgggcg tcggcttcga cggtgaaggt ctgcaggctc ggactgctcc
     3361 aggtggcgtt gtcccagccg cggaaaatga aggtcgcact atcgtcgctc atgcgggtga
     3421 tctggaagat accgccaccg ccctggccct gcaggttgcg ctggatgttg gccatgcggg
     3481 catccttcgg cgtttcaccc ccaccgccgc cggcctcgcc catcatggcg cgcttgcgtg
     3541 cgcgcatcat gttgatatag ctgctcatgt cgcccgggat ggcggtcgga gaggtttcag
     3601 ccggggccgg cggcgtcggt accggatgcg gcggtggcaa cgacaggcca tgatttcccg
     3661 gcgccgtcag gatgtgttgc tggcgcacgt gggcggactt gcgctgcggg cggacggtct
     3721 ggtgctcaat ccggatcgga accgatgggg tttgcggcgt cggtgcggcc gctggccggg
     3781 agggcatcag ttccacgttg agcggacctt cgctgccctg gcttgccggc ggtgccatga
     3841 ctggcgtgcg ccagtgcagc cagaacatgg ccagcaagtg cagcaggaac gaaaccagca
     3901 gggcaatgaa tagcgtgcgc ggaatgcgga tttcgacgta atgctcgggt gacggcagtg
     3961 cagcgcgcaa ttcgggaaac tcatcccgga atggcgaaat cttatggccg atgaacggaa
     4021 attgccgtga catgatgtgc tggagggcaa tggccctgac aagattgccc gcttggacac
     4081 cacggccggg gatttggttg cggcacggtg tccatcagcc cacttcccgt gtttcaagaa
     4141 actccagctc gggaaagcgc tcgcgcgtca tttccaggtt gacccggttg ggtgcgaggt
     4201 agacgtacag gccactgccg tcaagcgcca cgttgaaacc gtagcggtcg gctatctgcc
     4261 tgagtgcggt gtccggtccg cgcagccagc gcgcggtggc gaattcgcag ccttcgaaca
     4321 acacctcaac cccgtattcg tgctcgagcc ggtgtgccac gacgtcgaac tgcagggtgc
     4381 cgactgcgcc aagaatcagg tcgttggact gaatcggtcg gaacagctgc gtggcgcctt
     4441 cttcagcaag ttgttgcaaa cctttctgca actgttttat tttcaaagga ttttttattc
     4501 ttgccttgcg gaagaattcg ggggcgaacg aaggaatgcc ggtgaatttc agcgtttcac
     4561 cttcggtgaa ggtgtcgcca aggcggatag tgccgtgatt cggtatgccg atgatatcgc
     4621 cggcccaggc ctcgtcggtg gtgttgcggt cctgcgccat gaaggtgatg gcgttgttga
     4681 cggcaaatac cttgccgcca gcggattgcc ggattttcat accgcggtcg aagcggccgg
     4741 aacacacccg cagaaaggca atgcggtcgc ggtgttttgg atccatgttg gcctggatct
     4801 tgaatacgaa gccggtgaaa cgcgcttcct cgggcatcac caggcgagtc tcggtctggc
     4861 gtggcaatgg agcgggcgac agatccacca gcgcatcgag catcatctgc acgccgaaat
     4921 tgttgactgc ggaaccgaac agcacaggtg tctgtttgcc actcaggtaa tcggccgcgg
     4981 agaatgcatg cgaggcgccg cgcaccagct cgatttccgc gcgcaattcg ctggcctgcg
     5041 gtccaagacg ctggtcgagc agcggatttt cgagtccgtc gatgatttcg ctgatcgagc
     5101 ggtcggcctg cggatcgaag accagcacct ggtcgttctg cagatgatag atgccgcgga
     5161 agcgcttgcc catgccgatg ggccacgtca ttggcgcaca ttgcatgccg agcacggatt
     5221 cgatctcgtc gagcaggtcg atcggtgagc gcccttcgcg gtcgagtttg ttgatgaacg
     5281 tgatgattgg cgtgtcacgc atgcggcaga catgcaacag cttgatggtc tgcgcttcga
     5341 cgccattgac cgaatcgatg accatgaccg ctgaatcgac ggcggtcagc gtgcggtagg
     5401 tgtcttcgga aaaatcctcg tgtcccggcg tatcaagcag gttgatgatg tgctcgcggt
     5461 agggaaattg catcactgag gatgtgaccg aaatgccgcg ctgtttttcc agctccatcc
     5521 agtcggaagt cgcatgccgg ctggcttttc gcgcacgaac ctcgcctgcg acctggatgg
     5581 cgccgccgta ccagagcagt ttttcggtca gcgtggtctt gccggcatcc gggtgagaga
     5641 tgatggcaaa agtacggcga cggtggattt cctcgttcgg cgtcatggaa agcggggtgc
     5701 gggacaaacg gccattatag cagcatgctg cgtttgacgc agccgcggag caggttccga
     5761 ttgccgtcag t
//
LOCUS       DAIANL010000100         3825 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2576333, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000100 DAIANL010000000
VERSION     DAIANL010000100.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2576333"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          797 a         1345 c         1061 g          622 t
ORIGIN      
        1 gacgtgcggc cgacgctggc atcacagccc ccacgccagg ttgcgcaacg gaaattcggc
       61 aatgaacagc tgatgccggt aacgcgtgca atcgccatgc aggcagccag caccattact
      121 ggacggtacg acatatacac tgccatcagg aaaggtcaca tccgttgcca caatcttgtt
      181 cgacggatcc ggcatcagct cgcgcaccag catgcatacc cggaccgagc gcatatcggc
      241 aatcccgccc ggacaggcct gcgcgttgct gtaatatgta gacggatcgt tgtggcaact
      301 gacctggaag cgcagagcga cgacgccatc ggcgtattca tcgacactgc tgcccacccc
      361 gctctcactg gccagcagcg ccggatttcc gctctgcgcg ctcatcgaca acgcccactg
      421 ccacgtatcc ggctgcagct ggcccggcac accactgacg ttggtcacca gcgtcaggga
      481 ctggttctgg aaccctccgg gtgccagata attcccggcc acctgattcc gtaccacgac
      541 cggattgggc gtcgccccgc tggtcagatc cagcatgtag cccgcattct ggatgttcgt
      601 acccagcgcc agcagcgaaa tgcgtgcatc gtgccacatc gtgttgtcat cggtaacacg
      661 ccgcgccgcc tgggtcgtgg ccttgaccgc ggcccaggcg gccatgacca ccagcagtgc
      721 tatgaacatg ccgaccagca gttccaccag ggtaaatccg tgcgtacgca tcttcagtaa
      781 cccatgacct ggctcgtcac ataatctgcc tgcaaccggt tgctgccggt ccaggacatc
      841 gtcaccgtca gtacgcacgg tagagcagcg caggcaccac cgcccggcgc cgtcacacgc
      901 agggcaccgg caccggccgg caatgtctga cgcgtgcagg ctgaccattc gtccagcgcc
      961 tgctgcatcg cgccttgccc gccaccgggc ttcgccgttg ttcggttgtc cagcgcctgc
     1021 agcgcggcca ggttgccgga ggcctgcgca aacgccgcgg acagcatcat ctgcgccgcg
     1081 tacgccgccc gggtttcata ttccgcccgc ccggcctgcc gcagcacgtg caccatgagg
     1141 cctgccacgc cgagcattcc cagcagcaac accagcatcg ccaccagatt ttcgatcagc
     1201 cccactccgc tctgtgtctg ccgcatggct agctccggat aatttcgcta cgcccgcccg
     1261 cagtcaggct caggacccag gccgatggcg cctgaccgga tggcacgaca aactgcacac
     1321 tgaactgggg actgacctgc ccgagcacgc tgatggtgac cgggccggtc accgacggca
     1381 acaatcgcac cccacggaac tgctgaccaa agtccgccgc tgacatgcaa tgcgtcacct
     1441 gtccgccggg tcgcaacacc gaccactcgc tcgatccgca ggctgcaccc ccgatttcaa
     1501 gcaccagctt ctggttgtgc gccgcagcct gtgcctgtgc ccattgcaca tcctgcaaca
     1561 tgcgttctgc tgcgctgcgc gcacgccagt cagccagcgt gtcggccagc catggcacgc
     1621 cggccccgag cagcacgccc agcacaagca gcgtgactac cagttcgatc agagtaaaac
     1681 cgcccgtcca tctgtacacc gcattctccc ggtcgcatcg tgcctggcgc agcacacccg
     1741 atgtatcgca catgcagtat cacgcttgcc ccatcgccac tgacgcatga cgcatgacgc
     1801 atgacgcatt ttttcaattg caaccaacag ttcgatacaa aatatcacat attaacaaca
     1861 ataataatag cgctttccga ccagcgagcc tttccgaccg acaaacggat ctgcggcagc
     1921 gtcatcaagg cttcatgtgc aacgtccaga attcgcctga cgactggccg gatcaccgat
     1981 atgcggaata caccggcgcc tgccgtccca tacgatatgc atccggaatc ccatgaccga
     2041 aacggagcag gccgtcagca cggcatcaca gcgccctcgc cgaaaaaacg gcgcggagtc
     2101 cgggcttgcg cacccggcag atcgcgcacg cctggcaaaa gcaatgcagc tcgcgcaagc
     2161 acaggaaatc gccacgctga ccgaacagat cgaccgcctg cgcgagcaga tggccgatgc
     2221 agcccgcccc ctgctcgaac gctggcagcc ctggctgcac gatacagacc ggcgcatcag
     2281 cgcggaaaat ctggcctgtc atatcgcgct gcagcaatac gacctgcgtg aattgcagca
     2341 acgactggcg ctagccgggc tcgcttcacc gcagcatcac gaaactcacg tacacgccgc
     2401 actcgaagcc atcgcctgtg cgctgcagcg catggccgga aacactccgg gtgaatgccg
     2461 cagcagcgac ctgcgcctgg ccatgcaacg cgggccagcc acgcttgcgc acaatgcggc
     2521 acaattgttc aaggcggaca agggcgccgg ccatacaacc ctcatggtta ctctgccagc
     2581 ggcctgcgcc gacgatcagg cactggtccg ggaaatgctc gacgccggca tggattgcgc
     2641 ccgcatcgat tgtgcgcatg gcgcaccggc ccaatggcaa agaatggtcg acaacctgtt
     2701 tcaggccagg aaggagaccg gacgcgactg ccgtctgctg ttcgaactgg gcggcccggc
     2761 actgcatacc ggcgacatgc gcgcagccgc accgatcctg cacatcaaga tccgccgcga
     2821 cgaatatggc aacacgttgc atccgacgac catcattctc gacagcagcg gccagccagg
     2881 ccatccggcc acgccgctgc aaccggctcg catcgctgta gcagccgact ggctgcaccg
     2941 gttgcgccct ggcgatgcga tcagctttcg cgacctgcgc aggcgcaaac gcgaattaca
     3001 cgtacaggcg cgattgtcgc ccactgaagt cgccaccagt tgcaccgaag gtgcgtggct
     3061 gatcccgggt accctgctgg aacatgtgcc cgcaaaaaaa agtcatgcgg gcgccacgac
     3121 ctccagcggc gcgtttaccg caacccctga atacatcctg ctgcgcgaac acgaaaccct
     3181 gctgctgacg cggacgcagc agcccggtcg tgcggaacag cgcaacgcac gcaacgaatt
     3241 cctctcaccg gggcagatcc cgtgtgccga acccggcgca ctggacaatc tgcggccagg
     3301 ccacgaggtc tggttcgatg gcggccgcat cggcggtctg accgaacgca tcgatgatca
     3361 gggggtctgg ctacgtatca cccgtatccg tcccgaaggc gaacgactcg gctcacgcac
     3421 aagcctgcat tttcccgaca gcaatctgca gctgccggca ctgaccacac atgacctcgc
     3481 tgcgctcgat atcgccgtac cgcacgccga catcatcggc ctgccattcg tgcaacaggc
     3541 aggtgacatc gatcaggtgg cgaatgaatt cgtacgtcgt ggcgcagccc atttgcctct
     3601 gatcatccgg atcgcgaccc gcaccgcgat tgccgcgctg ccggacatcc tggtacgcgg
     3661 ttcggcacac gggccctttg gcgtacggat cgaccgggac agtctccgca cggaaaccgg
     3721 ccatcagcac ctgacagaaa tcatggaaga catgctctgg ctatgcacag ccggccatgt
     3781 accggtcatc tggacgacgc aggttgagga aagcccagac ggaca
//
LOCUS       DAIANL010000101        16279 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2577193, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000101 DAIANL010000000
VERSION     DAIANL010000101.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 16279)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16279)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16279
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577193"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2947 a         4529 c         5466 g         3337 t
ORIGIN      
        1 tgctcgacat tggcgcggac tggtgcgcac cgtgccgcgt gctggggcca cggctggaaa
       61 ggctggtgca cgaatacgcc ggaaaatttg tgctggccaa agtcgatgcc gatgaaaaca
      121 tgcgcattgc cggcagacac aaggtgcgtg gtttcccgac ggtgatcgcc tacagccgcg
      181 gtcaggagat tgaccggttc cacggcgcac agaccgaccg atttctgcgt gaactgatcg
      241 accggctgct cgcagagcat cagacatccg tctgagtgcg gggaaatcag cgcgtcgggg
      301 gcgatttttg ctcggggcgc aggcgcgagg caagcagcac gtcgacggcg tcggtatcga
      361 tggtctcgcc ggttgcgtca acgatcggtt gttcgaagtc attccagccg cggatgccgg
      421 tacgtagcga aaccacatcg gtgaatccca gtcgctgcag tgtgtcggcg gccaggacgg
      481 agcgtttgcc cgagcggcag atcacggcga tcggctggtg gcggtcggca gccagttcgg
      541 ggaccgtttc atcgtaatcc cattcgcagc cctgttcgag cacgccgcgc ggcaggttga
      601 ccgatcccgg gatgcgcagc tgactgtact cggcgggttc gcgcacgtcg agcagcagcg
      661 gcttgcgttc ggcggaatcc agcatccaga ccagatccca gggcatgatt tccttgaccc
      721 tcgacaaggc ttcagcgacg aggtcgtcgt aattgggcat gatggcgcgt ctccagatga
      781 cgtatgtcgg atgcggtatt gtaacccgtc gggctgtggt cgggtcagcg cagcaggatc
      841 agccgcggca cggcatcgaa acctgtccag agcagggcgt tctggtccca gcgccgcgcc
      901 agtgccttgc cggcttcgag ttccagtccg aacaccagca atccgggttc gccgtcggca
      961 gggcaacttg ggtggctgcc cgcgccctgc aggaagatga gcccgcggtg cgcaagatcg
     1021 ctctgcaatt ctgcctggcg cgcatggttg gtggcagggt cgagcaactg gctgcgtggg
     1081 ttccacgcgg tgatgaacgc gctgcagttg acgtgcctgg cccggtgcgc ctgttgcaac
     1141 gcggcgctga aggtctccac gcgcaggatg aaaggcgggg tgtcgtgtat ctggtaatcg
     1201 gattcgaggt aggcctgtac ggtctcgggc gcaatggcgg tgcctgatcc ggatgcggaa
     1261 gcagtggtca tgtgtgtact cctggccaac ggagagcgat acggtgcgca tggccgctga
     1321 ccgtgcgcga aattgcgtcg cgtgacagta ttttttgagt tatcgtacca tattcccttt
     1381 gggtttcctg cgctgcgggg tggtgtatca tggtcgggga cacggggcgg gcggggtcgg
     1441 atatggaagc atggctggcg gaacttggca ttgatccgcg ctgggtagcg ctgcgcggtc
     1501 tgccgttctg cgaagaggcg acggaactgg aatgcgtcga gacggcatcc gacggcaggg
     1561 agttcctgct cacgccacag gcagccgccg catggcggcg actgcgtgca gcagcgcagt
     1621 ctgatggcgt gcagttgttc gtgttttcgg cgtttcgcag cgtgacgcgt caggcggaac
     1681 tgatccgccg caaactcgac agtggcaagc ccctggatga gattcttgcc gtgcttgccc
     1741 cgccggggta cagcgaacat cacaccggcc gggcggtcga cgttggcacc cccgacagtc
     1801 cggcactcga aaccgagttc gagcgcaccg aggccttcgc atggctgcag cgacgtgccg
     1861 gcgagtttgg ttttcgcctg tcttatccat cgggcaacgc acagggctat caatacgaac
     1921 cctggcactg gtgtttcgtg ccgtgtgacg ccgccgcggc ttagccccgc cggtgtgcgg
     1981 tgtccggccg cagctgcggc agccgtgagt ctggcagatg gggcagatgg tgtatccggc
     2041 tgtcgacagt tggggcagac aggtctaaca tgcagtggag cagggtgggg gccgctttga
     2101 cggtgctgtg ccaaagctgg ccgcgtgggc gtggttgatc ggacggaggg tgcgatgaaa
     2161 cgagcagcgt atgcatgggg cgtggtgatg gtgttgatgc cggcgatggc ctgggcacat
     2221 cccgggcatc ccggtggtgg gttcttcgaa ggctggatgc acccgttttc cgggctggat
     2281 catgtgctgg gcatggctgc ggtgggtctg tgggcggcgc gcgaccgtcc gggtatgcgc
     2341 tggcgtgttc cactggcttt tcttgtcggc atgctggcga ggggaatcgc cggtctggcg
     2401 ggtcatgcgc cgtcctggat ggaaagcgcg ctggcagcga gtgtactggc tgcagcgatc
     2461 attgctatgc tggccctgcg catgccggta tgggtccgga tgttgctggc ggcaggcttt
     2521 gccgcgctgc acggcatggc gcatggcgtg gaattgcctg cagcggccga tccggccggc
     2581 tatactgccg ggttcctgct ggcgagcgcc gtgctgctca tgctgggtgc ctgggcggga
     2641 acccgcctgc aaaccggagc gcgggaccgc tggctggccg cattcatggg tggcctcgca
     2701 ctgaccctgt tctgaattcg agcgccggca tgcaaggctg ttcatgccgg ctatgttgcc
     2761 cgttcggctt tccaatgtga acgaactgtg aaagaatgca cagtaatatg gaaggttcga
     2821 ttgggtggcg gatatgatcg acaaggaagg gtatcgcgcc aacgtaggca tcatattgtg
     2881 caattcgcag aaccaggtgt tctggggaaa gcgtatccgg cagcattcct ggcaatttcc
     2941 gcaaggcggc atccagcccg gagagagccc ggaggatgcg atgtaccggg aactgatgga
     3001 agaaaccggc ctggaaccgc atcatgtgcg catcctggga cgcacgcgca actggttgcg
     3061 ctatgatgtg ccgcgccagt ggttgcgcag ggattgccgc ggtcattacc gcggtcagaa
     3121 gcagatctgg tttctgctgc gtttgctggg ccgggacagc gatgtttcgc tgcgttcgtg
     3181 cgagcatccc gaatttgatg cctggcgctg gaacgagtat tgggtaacca tggaaacagt
     3241 agtggatttc aagcgggacg tgtaccgtgc ggcgttgtcc gaactgtcac gctttctgga
     3301 ccgtgatgtg gtgccacgcc gccaggatgt ggtgtcggcg ctgctgcagc agagcgaata
     3361 gcgcatgaat ctgttgctgg aactgttcga ccgatcggtc taccacctgc cgggaagcag
     3421 cccggcagta ttctatcttg ccgatcgtga gggtggcggc atcctgatca actctccgcg
     3481 tttcgatccg gccttgctgg agcagttgcg ggcgatcatg cccatgagct atatctttta
     3541 tcccagccat ttcggctcgc ttgatgtgat ggcctggcgc ggggcgagcg gcgcgaagac
     3601 aattgcctac ggacatgagg cgaagcgggt cgatggcgtg atcgacatcg tgctcagccg
     3661 ggagaaccgg ttttcgcgca cgatcgattt cctgcccatg tctggccgta ccgaaagcac
     3721 ctgtgcgctg cgctgccgca acaagccggg catcgtgttt tttggcccga tcctgtccac
     3781 tgggccgtcg ggctggcccg agttgttgcc gcagtctgac gaccattcgt tcgagaaccg
     3841 ggtcattggt tcgctgggac tgcaggatgc ccgtttcgag tatgcgttta ccgatgattt
     3901 cgatccgcag ctgagccggt acggaccggg cgccgatgcg gccatacaac agcagttggc
     3961 cagcgtgctg gtctgatttg attgattgag aaagagacga gaagatggcg atttccgaac
     4021 tgcaggtaca gactgcgctg aagacggtgg tggacccgaa tacccacaag gatctggtaa
     4081 ccggcaaggc tgtgcgcaat ttgaaaattg atggcgacga cgtgtcgctg gatgtcgtgc
     4141 tgggttatcc cggggcaagc caggtcgaac cgatccggca gatggtggaa gaaaagctgc
     4201 gttcgatgac cggtattggt cgcgtcagcg tcaatgtctc gtcgaagatc gtgtcgcaca
     4261 gcgtgcagcg cggcgtcaag ctgatccccg gcgtgaagaa catcatcgct gtcgcctcgg
     4321 gcaagggcgg ggtgggcaag tccaccacgg ccgtcaatct ggcgctggcg ctggcagccg
     4381 aaggcgcgca ggtcggcatt ctggatgccg acatctacgg gccgtcgcag ccgaccatgc
     4441 ttggcgtgac cggtcgtccg gactcgcgtg acggcaagtc gcttgagccg atggaggcgc
     4501 atggcctgca ggtgatgtcg attggttttc tcatcgatgc cgaaacgccg atggtctggc
     4561 gcggaccgat ggtgacccag gcgctcgaac agttgctcaa cgatacgcgc tggcgcgatc
     4621 tggattacct ggtggtggac ttgccgcccg gtaccggcga tatccagctt acgctggcgc
     4681 agcgcgtgcc ggtgacggga gccgtgatcg tgaccacgcc gcaggacatt gcgctcatcg
     4741 acgcacgcaa gggattcaag atgttcgaga aggttggcat cccgattctg ggcgtggtcg
     4801 aaaacatgag tatccacatc tgtacccgct gtggtcacga agaacatatt tttggcagcg
     4861 gaggcggcga acgcatgtcc ggtgactacg aggtggaatt cctcggcgcc ttgccgctcg
     4921 acatccgcat ccgcgagcag gccgatggtg gcaagccgac ggtggtggcc gcaccggaca
     4981 gccggattgc ggagatctac cgtcagatcg cgcgccgcgt ggcggtgaag attgccgaac
     5041 aggcgcagga ccacagcaac gccttcccga aaatcgtgat ccagaacacc tgagcgatga
     5101 gcataaaatc cgaccgctgg atccgtcaca tggcggaaca gcatggcatg atcgagccgt
     5161 tcgaaccaca gcaggtgcgt gagcaggacg gccggcgcat cgtgtcctat ggcacgtcga
     5221 gttacggtta cgacatccgc tgcgcggatg aattcaagct gtttaccaac atcaactcga
     5281 ccattgtcga tccgaagcat ttcgatccca actcgttcat cgatgtgcgc ggagattcgt
     5341 gcatcattcc gccgaattcg tttgcactgg cgcgtactgt ggaatatttc cgcatcccgc
     5401 gcaatgtgct gacgatctgt ctgggtaaat ccacttatgc ccgctgcggc atcattgtca
     5461 atgtgacgcc gttcgaaccc gagtgggaag ggtatgtgac gctggagttc tccaatacca
     5521 cgccattgcc ggcgcggatc tacgcccatg aaggcgtggc ccaggtcctg tttttcgaga
     5581 gcgacgagga gtgcgatatc tcctaccgcg atcggggcgg gaagtatcag gggcaaagcg
     5641 gggtaaccct accgacaatc tgatgtgcct gctacaatgc gccaaccgga ccgtcggact
     5701 ttgcttctgg cggttttttc ttttgtgccg tgcaggcgtc ttcctggttc cgaggtgaga
     5761 acacatgaaa ttccgtttcc cggtggtggt gatcgacgag gattttcgtt ccgagaatgc
     5821 cagcggcctg ggtatccggg cgctggccaa ggcgatcgag gacgaaggca ccgaagtgct
     5881 tggcgtgacc agctacggtg atctgtcgag ttttgcccag cagcagagcc gggcctcggc
     5941 attcatcctg tcgatcgacg atgaggaatt ttcttcgccg actgctgccg cgcacgcggt
     6001 ggaacagttg cgtctgtttg tcgaggaaat ccgtttccgt aacgccgaca tcccgatttt
     6061 cctttatggc gaaacgcgga cctcgcgcca cattcccaat gatgtgctca aggaactgca
     6121 cggcttcatc cacatgttcg aggacacgcc cgaatttgtc gcgcggcaca tcctgcgcga
     6181 gatcaagacc tacctggatt cgctggcgcc gccattcttc cgcgcgctta cccattatgc
     6241 ttctgacggt tcgtattcgt ggcactgccc gggacactcg ggtggcgtgg catttctcaa
     6301 gagcccggtg ggacagatgt ttcaccagtt ctttggcgag aacatgctgc gcgccgacgt
     6361 gtgcaacgcg gtggaagagt tggggcagtt actcgaccat accgggccgg tggctgcatc
     6421 cgagcgcaat gcggcgcgca tcttcaatgc cgatcacctg tttttcgtta cgaatggcac
     6481 gtcgacatcc aacaagatcg tctggcattc cacagtggcg ccgggtgaca tcgtggtggt
     6541 ggatcgcaac tgtcataaat cgatcctgca tgccattatc atgaccggag cgattccggt
     6601 gttcctcatg ccgacacgca accactatgg catcattgga cccatcccgc tggaggaatt
     6661 ccgaccggag aatatccgca agaaaatcga ggccaatccg ttcgcacgcg gtattggcac
     6721 tcagccgcgc gtgctgacca tcacccagag cacctatgat ggcgtgctgt acaacgtcga
     6781 aaccatcaag tcgatgctcg atggtgaaat cgatacgctg catttcgatg aggcgtggct
     6841 accgcatgcg ccgtttcatg atttttatcg tgacatgcat gccattggtc gcgatcggcc
     6901 gcgctgcagc gaatccatgc tgttttccac tcagtcgacg cacaagatgc tggcagggct
     6961 gtctcaggct tcgcaaatcc tggtacagga gtgccagcaa cgcaagctcg accgcgatgt
     7021 gttcaacgag gcgtttctca tgcacacttc caccagcccg cagtatgcca tcatcgcatc
     7081 atgcgacgtg gcagcggcga tgatggaacc gccgggcggc acggcgctgg tggaagagtc
     7141 gattctggaa tccctcgatt tccgtcgcgc catgcgcaag gtggacgagg aattcggcga
     7201 ttcgtggtgg ttcaaggtgt ggggtccgga cactctcgcc gaggagggca tcggcgaacg
     7261 cgacgattgg gtgctgcgcg ccaaggaccg ctggcacggt ttcggtgatc ttgcgcccgg
     7321 gttcaacatg cttgacccga tcaagtctac catcatcacg cccgggctgg atgtcgatgg
     7381 cgatttttct gattcgggca tgccggctgc catcgtgacc aagtatctgg cggaacatgg
     7441 cgtgatcgtg gaaaagacgg gtttgtattc ctttttcatc atgttcacca tcggtatcac
     7501 caaggggcgc tggaacacca tggtgaccac gctgcaacag ttcaaggatg aatatgatcg
     7561 caaccagccg ctgtggcgtg tgctgcccga gtttgttgcc aagcacccac gctatgaacg
     7621 catgggtctg aaggatctgt gtgatgccat tcacggcatt tacaaggcca atgacattgc
     7681 ccgcctgaca accgagatgt acctgtcaaa catggagccc gccatgaagc cggccgatgc
     7741 ctatgcgcgc atggcgcatc gggccatcga ccgggtcgag atcgacaaac tcgaggggcg
     7801 tatcaccagc gtgttgctga cgccgtatcc gccgggaatc ccgctcctga ttcccggaga
     7861 gcgtttcaat gccactatcg tcagttatct caagtttgcg cgtgatttca acgagcgctt
     7921 tcccggtttc gagtccgaca ttcacgggct ggtcaaggaa gatgtcgacg gcaagcccgt
     7981 ctactacgtc gactgcgtca ggtaatcggc gcgccgcacg gtgttgctgc ggtatcagcg
     8041 ggttgtcgtg gtgcgcttgc gtcgcacggg ctttgccggt gcatctgcat cgaccggtgc
     8101 tggcgggaca accggtttgg ccagcggttc gatgggtgag ccaagtgcgc cgatgcgggc
     8161 ctcaaggatg ccgtcgcgtt gaggatctgg gtgcggcgac cgttgcagtg cggtgcatag
     8221 cagtggcggc agccaactgt ccgtagcctg aggtggcgtg acgtcgggcg catgccaacc
     8281 ggtcgacatg cccagcaacc agtcatgaaa agcaggatcg ggcggtggtg gaaaccggtc
     8341 ggctgtgtcg agcaggcgca attgccaggc cgtgtctgca agcaggaaag ctgcggcagc
     8401 gtggccaccg gcgcggtggc tgatggcctg gatggcacgt tgcaattcca gaatgcgctt
     8461 tcgggcatgg ccgtcgttaa agcggttcaa tgcgcgtcgg ttactgcggc gctgcaaggc
     8521 aaaccccagt ttcagcgaca gttgccgcag ttcggtttct gtcaggctgc cggaatgctg
     8581 cagtgcagca atttgagctg tgctgaaatc aaacagacgc agcagcagtg gggcgaaaag
     8641 atcaccagcg acgtcggtgc ggtagagtcc gaaacgataa ccgacactgg tgtctgtgcg
     8701 ggtaaacatg gcaattcgtc tggagtcgcc gtgatgcacg gtgtgcagga tcaacgcatg
     8761 cagatgagga atcagcagtg tatcgatctg tgcggtgctg aatgtgagtg ttggcggttg
     8821 gtaatccaca atggtccgca ggctgaggga atgcgttcag cctagcgaat gtgtgtgaca
     8881 cgctgatggc gcccatgacc acgcgtgtgg cgggctgccg cgcttcgaag tgtgtgccgg
     8941 gcacacgtgt catgtgcccg gggctggtca gatcctgccg ctgctgttct gttgtgtgcc
     9001 cattgcggag ggcgtttgag tgacagaagg catgggttgc tggctggccg gtgtcagcag
     9061 ggccgccaga ccggagtcgg tcgacggact gccagacggg gaggggctgc tgaacagtgt
     9121 ggacagcgtg ttgtcaatgc tggcagtcac tgtctgttcg agttgcgcca gttgcgtggc
     9181 attgacctgg ccgcgggaat tgtaggtcag cggcgcggca acgccattac cgaggctcac
     9241 tatggtgctg ctggtgttcg ccgcgctgct cgtgcccgaa gcatcctgtg cggtcgtgct
     9301 cgacaggatc tggttgatgc cttgttgcaa tgcgctgttg ctgagcgtgg agatggagct
     9361 catggtggca ctcctggttc gcagacgtaa ctacagatat caccataacg gcgcaaaaaa
     9421 tgaaaagttg agggccggac gaaatgtatc ggctgatggc agccccccat catcggcccg
     9481 gtagttcggc cggggagccg gctggcggca ggccaccagc cgcatggggc gttattgctg
     9541 ttcggcaggg ggtacgtagc tgccgggagg ggggccgtag gttgcgccgg gaggcggcgt
     9601 gtagcccgat gagggtggcg gcggcggcag tgaacttccc ggctgctgcg agtagccgcg
     9661 catgaccgat ccatgaaccg gaacctggtt gcctttggca tacatgcact ggacgtaggc
     9721 attgtcatac tgacgttgcg ttccataggc cgactgttgc gaggtgcctg tgcccaccag
     9781 ggtgccgccc agcaggccaa tgccggcacc ggtgccggcg ccttcgtggc cgcccatcgc
     9841 agcgccggcc agcgcaccga cagctgcgcc gacggccgcg ctaccgacct gggagcggat
     9901 tgcggcatcg ttggcggtag agccgccgac ctgggcggag gcgtattgcc ggcaaatcat
     9961 gtcatccatg cggaactggt caaagctctt gccattgccg ggcaatgcca tgacagacgg
    10021 cccggtcggt accgtggtgc aggcggcaag caacgtcagg caagacagcg ctcccagttt
    10081 gagccagaca ggtttcattg tgattctcct tttgttacgc ggtcgtatga tgcttaatgg
    10141 cgactcagtt gcccggaacc cccggaggta ctgctggaac cgctcgccac ggctgcgggc
    10201 agttcgacac atacgggtaa aacgccttcg ccgagtcgca gtagtaccag gtgttggctg
    10261 gcgcggtggc gggcgcgctc gtcaccggtg cgggctgctg caccacgacg gtttgcggat
    10321 aggccgcctg cggatagccg tacgggtaat agtagggata ataataggga tagtaagggt
    10381 aggccagtgt cgaaccgatc acgactccgg tcagcagacc agcgcctatg ccccagccat
    10441 cgtaccaccc gccgccgcgc cagccatcat gccaggcacc accgcgccag gcgccaccac
    10501 cgtgccaggc cccgcctgga tgtccccagg catgagctga aagcggtacg gtgctgcagg
    10561 ccagagcgat cagcagcgcc gccgcgcgtg taagaaagat tttcatggcg atgccctttc
    10621 attgcggtcc gctggcagaa ggcggccggg gagtgtcctg cccggggtgc cggtccggga
    10681 caaccatcgg gtgaccgtaa tgacgcatca tcatgccatg gtggaattgc gttgcatgtt
    10741 ccagacgcca gaacagatcg aaaatcgttt tttgcttgcg gtcgagcgtg gcatagaaat
    10801 tcgcagtgtt cctttcggca gcctccacac tggccagctg tttctgcagt ccggcgattt
    10861 cgtcgcgcag gtcgttttcc tggtgttgca tcagcgcggg cgtatccagc gcgtcactgt
    10921 tgtccatgcg ctcggtccat tgctgcaggt tgtggcactc gtctgttgac tgtttgcgca
    10981 catcggagag tacggtcgca gaccaggcat tccaggcagc gagctggtcc gcccggatgt
    11041 cgagtttctg tttcaggtcg gccaggtggt cggtcatcag gctggcgcgg tcttgcgtgg
    11101 cgtaggggca ggcttcggat gcgctggtgt cggcgtcggc cagggccgaa gtggcgggca
    11161 gcagcacaag cagtgtcatc aggtggaaaa gtatgcgtgg tctggatgga atcatgacgc
    11221 ctcccgtcgg gtcattcgca ccgtttcttc gttgttgaat ccggagcagc agcgtgtcgg
    11281 tcataccgca ggcagggggt gcgaaaccgc gccggcagtt gtcgctgcgc agtcgcctgg
    11341 ccatacgggg gagactgcca aacgcagcaa aagttccttg tcatgcacca tcatacgccg
    11401 ctcagtgcgg gtttttctgc ccatcggcgg taaaataata ggtgacgtgg gtgcgtgggt
    11461 tgaggtactc gcgtaccgcc gccggcaacc tggcgaggcg caaacctttt tcgattttca
    11521 gttccgattc cgttccaaga ttgatgacca gggcttcgtc acgtggcacc gcagggtcat
    11581 agaccaggat ttccggccgc aatgggtcgg cggggttgat ggacagcacg agtccgggca
    11641 tggcgttgtt gagctgaacg atgctgccgg gcgggtagac acccattagc cggatgaagg
    11701 tgctgagcat gtcagcgtcg aactgcgtgc gccgctgggc gaacatgcgc gccagggctt
    11761 cggctggtgt gctggcctcg gtgatctgca gcggattgca caggttgtca tacgtgttgg
    11821 cgatgaccag cgcccgtgcc agtggggaga tctggccgtt ggacagccgg ttcggataac
    11881 cgctgccgtc cagatgttca tgatgctggg caatggcggt cagcgcatct tcggagacgc
    11941 cgattttgcg cgcgaatgcc gccccgatga cgcagtgctg ctcgagcaga tgctgttcgg
    12001 gtcgggtcag tggttcggtt ttggccagaa ttttgtgcgg cacctcgctc ttgccgatgt
    12061 catgcagcat ggctgcgatg ccgagttgct gcatgtcgtc ggcgccaagt ccgacagcgc
    12121 gacccagcat cagtgacagt acggtgacgt tgagtgagtg gaaatagacg tcgtcaccaa
    12181 gtttgttgcc catggcgtgg atcagcaggt cgccatcgct gaggatgctg ccgaccatgc
    12241 gttcgaccag atcgacggtt gcctgcaagg tttcttgcgg gcgggtgtgc aggttgcggg
    12301 aaatgttgcg cacggtgtcg gcggtacgca tgaattcgta ttctgcgcgg gcgatgcggt
    12361 tgcggatttc gcgcagtcgt tccacacggg ccagtcgcgc gacatgctgt gcctgcgctt
    12421 ccgggtcggg tgctggttcg ggggcgggcg ccactctggc cggtgcgctg gcgtgcagcg
    12481 gttcagcgtc gctgcgcgcc ggatcgtagc tgatgcgggt cagccccatg cgacgcaatg
    12541 tatcgatctg gtcctggttt ctgatcttga agtgactgaa caggaacggg tgtttcatcc
    12601 agtccaggtc aagatggacg tacagcccga tactgagctg gtcgggcgaa atgtgcggat
    12661 agtcgggcat ggctgacagg ataaggcgga accgggcaac agaatgactg catgatcact
    12721 tattaccatg tttgccagat ccttacaagc ggtttgtgtc agcatgagtt caggttttgc
    12781 gccacatgct gagcatgacc gagccgatca ccagcgctcc gccaccgagc tggatcagat
    12841 tgggcagctg acccaagagg ctggccgtga gcatggcaaa taccggaacc aggttgagga
    12901 acagcgcagt gcgagccgca cccagttcgg cgatgctgcg gttccacaac atgtaggcga
    12961 caacggtgcc tgcggtgacc atgagcgcca gcgccaggcc gccttgccag ccaggcagcg
    13021 tgagcggctg gccgctgtag aacgcggcgc acagcagcat gagggtgccg gtgcccatca
    13081 gcagcgtggt gttgatgagt gtcgattcgc gtggcaggcg gcgggtgagc aggttatagc
    13141 ctgcccaggc caggttggcg cccagcatca gccagtcgcc gcgcacgatg tccagttgca
    13201 gcaaccgggc cgggtggccg tcagtgatga ccaggatgac gccggagagc gccaggggaa
    13261 ttgagaacag ccagatggtt gatgggcgct cgccgagcag gagcgctgcc agcagggtgg
    13321 tcaggagtgg attggtggcc atgaccagtg ctgcattggt cgaagacgtc gtttgcagcg
    13381 cggtgaagaa cagcaggttg aagccgccga tgccgagggc gccggcgagc aggtagacag
    13441 gcagatgcct gcttgccatg gcgaacatgg cgcgcatgtg ccggaaagac cacaacagca
    13501 tgatcagtgt accaaaaccg aaccgcagcg aggcggccca cagcggaggg aggtcatgca
    13561 ggatcgggcc ggccagtaca aaattggcgc cccagaacac ggtggccagc agggtgctga
    13621 gatatactct cggtatcatc ggtcgtcagt ccggtataca aggagtcagc agtctagtgg
    13681 gtatgcggtc atggggcaaa ccagtctttc tgaatgccgg agcaagaaaa tctgatggta
    13741 cgtagcctgc cttcgctcaa tgcgttgcgt gcgttcgaag cggttgcccg cctggggtcg
    13801 attgcgcgcg cggcagacga actgcacgtg acgcctgctg ccgtgagcca gttggtccgg
    13861 cagctcgagg actacctggg ggtgagtctg atgcagcgcg gccgcaggct ggtgttgagc
    13921 gagcaggcgc gagccgcgct cccctggctg agccgggggt ttgatcacat gcatctggcg
    13981 gtcgaacaca tgcgtccgcg acgggaggat agtacgctgg tggtgaccgt gccgccggag
    14041 tttgccgcgc gctggctggt gcagcgactg gaagatttca gccgccagtt ccccgaaata
    14101 gaactgcgtg tgcagccgac ccgccggctg gtcgatttca ccagcgagtc ggtcgatgtt
    14161 gcgatccgtt tcggcagcgg cgaatttccc ggcctgttta gcgaattgct gatgccggaa
    14221 tcgatcgtgc tggtggccac cccggagctg gctgccacga ttggcgcacc ggcggatctg
    14281 cccggctgca cgctgctgta cgatgatgcc gagacctggg atccgttgtt tcccgactgg
    14341 cagcaatggc tggccgcgca gggtgtttct gccggggaga tgctgcgcat tcgtcatctc
    14401 ggtacggtcg ggctggtgat tcaggccgcc accagcgggc tgggtgtggc actgatgtgg
    14461 cgcacgctgg tgctggacga attgcgtcgc ggcagtctgg tgcagctgtt tggtctggag
    14521 atgccgagcg gtcgggccta ccatctggtc gtgcccgagc cgcgtcttgc actggccaaa
    14581 gtcggcgcgt tcaggcgttg gttgcggacg cagtcggatg gcggttgacg tgacagggta
    14641 cggctgatta caatacccga tggtttttgt cgaatatttt tgtttttgga gcggtcatga
    14701 gcatacagga caccattcgc gagcaggtta ccagtcacaa ggtcgtactg tacatgaagg
    14761 gtacaccaca gtttccgcaa tgcggttttt ccgccaatgc catccagctg ctcaaggcgg
    14821 cgggcgtgaa ggatctgttt actgtcaacg tgctgatgga acccgaaatt cgccagggta
    14881 tcaaggaata tgccaactgg ccgaccattc cacagctgta cgtcaacggc gagttcgtcg
    14941 gtggctcgga tatcatgaag gaaatgttcg aaagcggcga gctgcaatcc atggtcgccg
    15001 ctgcctgagc ctggacgtgc gacgtccgtc cgtatctgtc tgacggacgc ggggtcgtgc
    15061 gcggcctgtc aggcggatcg gtgcggagac cgtccacgcc tggtcaggac gtctacgccg
    15121 ccgggctggc cgtttcggcc tgttgcgcga cccgggcggc gatgtgcgcc agcgcctcgt
    15181 ccacctgatc gatcaggatc aggcacagat cgccgggttc aagtccggca agtgccagat
    15241 cgatggcgtt gaattccccc tgtatttcat cgatctgccg ggtccgctgc gcgccggtca
    15301 ggccctcgcg cagcagtcca agcacctcgc cgtcggcgcg accgcgttga cactggtctt
    15361 catacagaat cacccggtcg aaggcggcgc cgagaatcgc ggtctgctgg cgcaggtctt
    15421 cgtcgcgccg gtcacccgcg ccgctgatga ccacgctgcg gcgtttggct ggcaacgcgg
    15481 agatcgcatc gaccagtgcc aggatggcgt ccggattgtg cccatagtcg gcgatcagcg
    15541 ttgcatcgcg atattcgaac aggttgaagc ggcccggcgc cgtcgcggca tcgttgatga
    15601 agctcgccag tccgcgctcg atggtgccgt ggtcgagccc gagcgcccag gcggcaccga
    15661 tcgcggccat gacattttcg acctggaacg gaatgctgcc attgcgcgtg agcggaatgt
    15721 tatcgagtgc aaagcgcagc acccgtcgtc ccttgcgggc gaccagatcg ccgccgtcgc
    15781 ggtaaatcac tccatggccc tgtgccgagt gggtgacgac aagcggatgc aacctgtcct
    15841 gggcgaagaa aatgaccttg ccggggcagg ccgtggccat cttggccacc atcggatcag
    15901 cggcattgag taccgctgtg ccgctggcag cgacattttc cacgatgacg cgcttgacga
    15961 ccgccagttc gtcgatggtg tggatgtagt tgagcccgag gtggtcgccg atgccaatgt
    16021 tggtgacgat ggcgacatcg cactggtcga acgccagccc ttcgcgcagg atgccaccgc
    16081 gtgcggtttc cagcacggcg gcttcgacct gcgggtgggc aagcacattg cgggcgctct
    16141 tcggaccact gcagtcaccg gtgtcgatgc ggcgatcgtc gatgtagaca ccatcggaat
    16201 tggtcatgcc ggtgcgccag ccattatgac gcagcagatg ggcggtgagt ctgacggtgg
    16261 tggtcttgcc gttggtgcc
//
LOCUS       DAIANL010000102         9756 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1361354, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000102 DAIANL010000000
VERSION     DAIANL010000102.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9756)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361354"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2039 a         3268 c         2790 g         1659 t
ORIGIN      
        1 ggccatcgtc aacccctacg acctgttttc gctcgaagcc gctctgcaac tgaaagaccg
       61 gcatggcggg cgcgtcaccg ttatcagcat ggggccgccg caggccgagg cagcactgaa
      121 aaaatgcatc gcctacgggg cggatgaagc catcctggtc accgaccgcg cctttgccgg
      181 cgccgacacg ctcgccacct cgtttgcact ggcgtcagca atccggcaga tcgaaaaaga
      241 cctccgtgtc gatctggtgt tcaccggcaa gcaaaccatc gatggcgata cggcacaggt
      301 cggcccgggc attgccaggc ggctgggcat gcaactgctc acctatgtct cgcagatcga
      361 cacgctcgac ctcgatgccg gcaacatccg cgtgcgccgg cgtgccgaag gcggggtgca
      421 gttgctggaa acccggctgc cgtgcctgat caccatgctg gaaggcacca gcgacatgcg
      481 tttcgccacc atggaaaaca tgtttcgcgc cgcacgctac cagacccgca tctgggacaa
      541 ggatgccgcc ggcatcgaag ataccggcaa gatcggcctc aaaggctcgc ccacgctggt
      601 cagcaaggtg ttcgcaccca agccgctttc acgccgtgcc gacatcatcg acaccgagag
      661 ccacgagccg cgcgacctcg cactgaccct ggttggcaag ctgttcacgc agcatcccga
      721 actgcgcgac agcctggcgc aacaggacgc atgaatccga caccgaagca aggaacagac
      781 atgactcaag ccaatccggc accggcagcc agaaaaccag ccgggcgcgc aagctacgaa
      841 ctcgacgacc ggctgaaagc ctaccagggc gtctgggtat tcatcgaaca cgagcagggc
      901 catgtgcacc cggtatcctg ggaactgctc ggcgaagggc gcacactggc cgacacactg
      961 ggcgtgacgc tctcgggcgt ggtcatgggc gctccgggcc tgcccacccg gcgtttttgc
     1021 gaggaagccc tgttccacgg cgccgataac tgctatctga tagcggacag cgtactggcc
     1081 agttaccgca acgcaccatt caccctcggc ctgacccatc tggtcaacca ctaccagccg
     1141 gagatcctgc tgctcggcgc caccacccag gggcgcgatc ttgccggcag cgtagccacc
     1201 acgctggaaa cagggctgac ggccgactgc accgaactca gcatcgatat ggacaaccgc
     1261 ggactggccg ccacacggcc gacgttcggc ggcagcctgc tgtgcaccat agtcacgcgc
     1321 aactatcgtc cgcaaatggc gactgttcgc ccccgcgtca tggcgatgcc cgcgcgagac
     1381 ccatcgcgtt cgggccacat catcgaattt ccgctggaca tggtggaaga cgaaatcgtc
     1441 accaaggtgc tcgaatacct gcccgaccag acacagggca aaccacaact gccctttgcc
     1501 gacatcatcg tggcgggggg gcgcgggttg aagaaaccgg aaaatttcca gctggtgtgg
     1561 aatctggcac gggtgctcgg tgccgaagtc ggggcaaccc gcccggtggt acaggccaac
     1621 tgggtggaca gcgagcgcca ggtcggtcag tccggaaaga ccgtgcgtcc caagctctac
     1681 atcgcagccg gcatttccgg cgctatccag catcgcgtag gcatggaagg cgcagatgtc
     1741 atcattgcca tcaacaacga tccggatgcg gccatcttcg agtttgctca ttacggcatc
     1801 gtcggcaacg ccatgaccat tctgccagcg cttaccgaaa cattcaggca gcagatcgcg
     1861 ctggcgcgca aggccggctg acacaggagg aaccgacatg gccgaacaat ttgacgcaat
     1921 cgtggtgggc gcaggccccg ccggcaacgc cgccgcctac acgctcgcca aatccgggct
     1981 cagagtgctg caactcgaac gcggcgagta ccccggctcg aagaacgtgc agggggccat
     2041 cctctacagc gatgcgctcg aacgcatcat tccggatttc cgcgaagatg cgccgctcga
     2101 acgccacatc atcgagcagc gcatgtgggt actcgacgac gaatcattca tcggcgcaca
     2161 gtaccgttcc gacggtttca acacgccgcc gtacaaccgt tacaccatcc tgcgtgccca
     2221 gttcgacaaa tggttttcgg agaaggtgca agccgccggc gccctggtca tctgcgaaac
     2281 cacggtcacc gggctgctga tggacggcag gcgggtcatc ggtgtgcaga cagaccggcc
     2341 cggcgggtcg atctatgccg acgtggtgat cctggccgac ggcgtcaatt cgctgctggc
     2401 gaaaaaagcg ggcttccatg ccgagatcga tccggccaat gtggcactgg cggtcaagga
     2461 aatccatttc atgccgcagg aaaccattga atcgcgcttc aatgtcagcg gcgacgcggg
     2521 cgtggtgatc gagatggccg gcaagatcac ccgcggcatg atgggaaccg gttttctcta
     2581 caccaaccgc gaatcgctga ccatcggcgt gggctgcatg ctgtccgatt tcaaggcaca
     2641 gcatgtctcg ccctaccagt tgctggaaga catgaaggcg catccgtcca ttgcgccgct
     2701 catcgccgac ggcgaaatga aggaatatac cgcgcacctg atccccgaag gcggctacaa
     2761 ggcgatcccg aagctgtatg gcgaaggctg gatgatcgcc ggagacgctg gcatgctggt
     2821 aaacgccatg catcgcgagg gctccaacct cgccatgacc tcgggccgct tcgccgcaga
     2881 gaccgtcatc gaactcattg ggcacggcaa gtcgtgcagc gcaaccaacc ttgccgccta
     2941 ccagcgcaag ctgaatgact cgttcgtcat caaagacctg aaaaagtacc gcaacatgcc
     3001 gggtgtgttc cacaccaatc cgcagttttt caccacctat ccggaaatgt tcaaccaggc
     3061 cgcgcaccgc atgctgcgcg tcgatggcat cgacaaaaaa tccaaggaaa gagccattct
     3121 ggctgatttt cgccagcgcc gctcgctgtt cggcctgatg agcgatgcat tcaaactgtg
     3181 gagggctttc gaatgaacgc acgggtcaaa gtcgaagaga aactgtttca gaaccgttac
     3241 cgcgtcgatg gcggccaccc gcacatcagc attcgtaacg aggacatctg tgccagccag
     3301 tgtcaggacc gcccgtgcac cgtctgctgt ccggccggct gctacaccat ggaaggcaac
     3361 ggcagggtcg tactgatcac ggacggctgc ctcgaatgtg gcacctgccg catcatctgc
     3421 accgaccacc gcaacgtcga ctgggaatat ccccggggtg gttatggcat cctgttcaag
     3481 ttaggctgac acacccgttt tccgtagccc gattccacac cgaggtcatc atgagcaaac
     3541 ccgtcaaaca tgtattcatc tgtgcgcaaa accggcctgc cggccatccg cgcagctgct
     3601 gcgcccacaa gggcgccgat gccgtactgc aggctttctg gcgagaactg cagcaacgca
     3661 atgcctacga tcgcatcgcc gcgacctggt ccggctgtct gggtccatgc gacagcggtg
     3721 tcaacgtggt ggtctatcct gaaggcgtgc tgtatcaggg cgtgacggtc accgatgtca
     3781 gcgaaatctt cgaccagcat ctggtcagcg accagatcgt cgaacggttg cgcgctccgg
     3841 cctcagtctg gtaagcgacc ggcgcgatac gacgccgcag tgcctcctga agccggccat
     3901 ccgccggctg tattttcccc caggcgttgc aattgatacc aatcatgata tcattggtat
     3961 caattaatga tgcacggaaa tggagacgca cgttgacacc tcccctgcca cgccacccac
     4021 ggacccggtc acggcgcatt cccacaccgc ccatgcacct ccctgccgga ggcgcgcgac
     4081 catgatcctg tccaaactgc tcaacatcat ccacgaacgc cccaccgtga gcgacatcca
     4141 tatctgcgcc ggtgagccgc tgtacatgcg cagcccgtcc ggttacgacg cctatgacac
     4201 ggaaccggtc agccacgagg aactcgagga gttcatcgcc agcaacgcca tcggcgggca
     4261 aaactggcgc accgaactca tggcgcaagg cggcaagctc gatggcgcgg tcgacctgac
     4321 caattcgcgt gtgcgctatt cgatctacga aaccaatggc atatcgcacc acatcaacgt
     4381 ggtcatgcgc atcatacgca acaccatcat tccgctcgaa cacctgggcg caggtcagca
     4441 catgctcaag gaactggtca tgcgcggcaa ggggctggtc atcatcaccg gtccaacggg
     4501 ttccggcaag accaccacgc tgtcgtccat catcgaccgc atcaattccg agcgctcgtg
     4561 ccacatcatg accatcgaag acccgatcga acacgtgcac cagaaaaaaa ccgccctgat
     4621 ctcgcagcgc gaaatcggca ccaacgtcga cagcttcgcc agcgggcttg aaggtgccat
     4681 gcgccagcgt cccgatgtga tcgcggtcgg cgaactgctc gatcgcgaca ccgtcgaaac
     4741 cctgttccgc gccgccgatt ccggccacat ggtactggcc accacccacg gccgcagcgc
     4801 cagggatgtc atcaaccggc tgctcggctt cttcagcacc gacgaacgga ttcagcgccg
     4861 ccaggtactc gcctcctgtc tgagctgcat catcagccag gtgctcgtgc cgtcgatcac
     4921 gcgcgaggaa tgggtactgg cttcggaaat tctcgtcaat tccgccggcg tgcgctcgat
     4981 catcgccagc ggcaacctgg cgcaattgcc ggcagcaatg caacaagggc gggctgaggg
     5041 catgaatctg ctcagcgccg acctggcacg actggtgcgc gaaaaccgca tcagccagca
     5101 acaggcacgc atggcagcct acgatgaaag cctgtaacgc tttcgcaagc aaccaggcag
     5161 tgctgcgtca agcgcctgag atttgcgcgt ctccatcggc ggcctgtaac gcctcggcaa
     5221 gcaaccgggc agtgctgctg gcgctgaccg tgctgctctg ctccggcccg gctgctgacg
     5281 gcctggcggc caccagtttc tgggaccggc actacaatcc cgccaacggc caggaacgtc
     5341 ccggcgcacc tcccctaccc gagttgcatg tgctctacac acgctatcat ccatccagcg
     5401 gcaaaaccct gactggccgt acccacggct acgggcaccc agcctggctg ctgcgacaac
     5461 tggatgcacg gcaaccgcaa ctgcgcgccc agggatatga cccgccgcgg ctggtgcgtg
     5521 cctcgatcaa ccgccaggtc atccgtaacg cccagcacac gctggcagac cagtatcgca
     5581 gccaggacag ggaacgccgg acatccggcc tgcctgcagg ccatgcgccg cgcagcaaat
     5641 gaacgcattg agcgcagcga atgggaacgc accaaccgcc acagagcgac ggcatcaggt
     5701 acactatgct gcccgacgat cacttccgac ccgaccgcac tgtcatgacc gatccagcca
     5761 gtttcgcgct tgccatccgg cacaccgttc ttgaagccct gcaggaagat ctcggcacag
     5821 gcgaccagac ggcagcgctg atcgctgccg acaccacggc gcatgcgcgc gtcatcaccc
     5881 gggaacccgc cgtgctgtgc gggcaaccct ggttcgacgc ctgtttcacc ctgctcgacc
     5941 ccgaagtgaa cattacctgg catgcgcggg acggcgaccc tgtgcaggca ggggacacgg
     6001 tatgcgaact gcacggccat gcacgtgcgc tgctgagcgg cgaacgaccg gcgctcaatt
     6061 ttctgcagac gctatcggcc acggccacac tgacacggcg ttatgtgcag gccatcggcg
     6121 actacccatg ccaggtcatg gatacccgca agaccctgcc gggcctgcgt ctcgcgcaga
     6181 aatacgccgt acgcgccggt ggcggcagca accagcgtca cgggctgtac gacggcatcc
     6241 tgatcaagga aaaccatatc cttgccgcag gcggcattgc agcggcgctc gcggccacac
     6301 gccaggccag cgtaccggta cagatcgaag tggaatcgct ggatgagttg cgtcaggcgc
     6361 tcgatgccgg cgcccccctg atcctgctgg acaatttcga tctcgccacg ctgcgcgaag
     6421 cggtacggct ggccggcggg cgtgccgtgc tcgaggcttc cggtggcgtc acgctcgaca
     6481 cgatccgcga catcgctgcc accggcgttc aacgcatctc ggccggcgag ctcaccaagc
     6541 acatccgcgc catcgatctg tccatgcgca tcgtctgacc catgccggat cgcgctgcca
     6601 ccctgtctgt cgtcatcccc ggtctgatcc cccctgccgg catgactgcc gcggtactgg
     6661 aaggactgac gctgcccgcg ctcggccgcc tgatggcgcg tgccgaacgc atggcatgtt
     6721 ctcagccggc cgatacggaa agtctgctgt gccagtcgct gggcatcgac cgccagaccg
     6781 attggccaac cgcccccatc gacctgcaag ccgaaggtgt gcctcccgga tccgccttct
     6841 ggctgcatgc cgatccggtc cacctgcgtg ccgcacgtca cgaactgata ctggtcgaca
     6901 gcagcgcttt cgctgtcact gccgccgaag cggcgcaata tgtcgccgcc ttcaatgacc
     6961 acttcggtgc ggaaggctat cagcttgttg ccccggaccc gcaacgctgg tatctgcgcg
     7021 tacccggcca gccagtactg gcgacccgca gcctgcacct tgccgctggc gcggccatca
     7081 atcccgcatt gccacaaggc gcggatgcgc tgcgctggca tcggctgttc aacgagatcc
     7141 agatgttgtt tttcgatctt ccggtcaacc ggcagcgcga ggcgcgcggc gaagctccca
     7201 tcaatagtgt atggctgtgg ggcggcggta cgctgccgcc tcctgtccct gagacgcgga
     7261 ccggcgtgct gtggggcaat ccgcgcggct ggcgcaacct ggccacgttc gccggactgg
     7321 aaactcacgt cctgcccgac accgcacccg gccgcctgcc aacccagctg gactggctta
     7381 ttctcgatgc gctgcacgac agtgcccgct acgccgacta tacccgatgg aaaaccgcgc
     7441 tgctgcaact ggaacagcac tggttcgcac ctctgataca tcatctggcg accggacaga
     7501 cacgcatcct cgacctgtgg agttttcacg aaggccatgg cgtacgctgg cggatacatc
     7561 gcctcgacct cgcacgactc tggcgtcgta cgccgctgat gcacgcactg cggccacccg
     7621 ccagcgtctg agccaggaca ccccggacta cttggccata ctggccagca acaccgcctg
     7681 tgccgtcacc ggacggccgc ggccggagac ccgtcgcaca tactggccat cggatcccat
     7741 gcgccaggct tgctggttat cacgcagata cgggcggata ccttcattca acacacgttt
     7801 tttcagccgc tgatcaagta tcggaaacgc gatttcgata cggcggaaga aattgcggtc
     7861 catccagtcg gcgctcgaaa gatacacgtc gtccttgcca tcgttacgga agtagaatat
     7921 ccggctgtgt tcgaggaagc ggccaatgac cgagcgcacg cggatattgg tcgacagccc
     7981 ctcgacacca ggccgcagcg aacaggcgcc acgcacgatc agctcgatgc gcacccccgc
     8041 ctgggctgcc tcatacaatg cgtggatgat ctccggttcg agcagagcat tcatcttggc
     8101 cacgatcaga ccgcgccggc cttcacgcgc atgcgcagcc tcgagacgga tcgcccgcag
     8161 catgtggctg tgcaggctga acggcgactg ccacaaccgg cgcaggtgcg acgcgctacc
     8221 cagcccggtc agctgggtga acacctcgtt gacgtcggca cagatgtcct cattgcaggt
     8281 aagcagacca aaatcggtgt acaaccgtgc cgtgcgcgca tggtaattgc cagtacccag
     8341 gtgcgcgtag cgacgcaacc cgcccgcctc gcgccgcacg accagcgcaa tcttggcatg
     8401 cgtcttgtaa ccaaccacgc catacacaac atgcgcgccg gcctgctcca gccgggccga
     8461 ccagttgatg ttggcctctt cgtcgaaacg ggccatcaac tcgaccacca cggtcacttc
     8521 cttgcccgcc tgagcggcgg caatgagcgc ttccatcagc atcgaatcgg tacccgtgcg
     8581 atagacggtc tggcgaatgg caagcacctg cgggtcctgt gcagcattgc ggatgaaatc
     8641 gatcaccggc tgaaaactct ggtaaggatg atgcagcaac acatcaccct tgcgaatgac
     8701 cgcaaacaga tcgttgcttt tggccagcac ggctggcagg ccgggcacga atgcagggaa
     8761 tttcaggtca ggccggtcga cctggtcggg gatctgcatc agccgcacca gattgaccag
     8821 gccattgacc tgatagaaat cctcttcgcc caggccgaac tgctgcaaca ggaagcctgc
     8881 catgcgttcg gaacagttgt cagccacctc cagccgcacg gcatcgccga aattgcgctg
     8941 cgacaactcg ccctgcagcg cttcacgcaa attcttgact tcttcctcat caacgaacaa
     9001 ttccgaattg cgcgtcacac gaaactggta gcagccttcc accgtcatgc cgatgaacag
     9061 ctcgttgacg tgggcatgca gaatcgaaga caggaacacg aaaccgtatt cgcatccggc
     9121 cagttcggct ggcatgcgga tgacgcgcgg cagcgagcgt ggcgcctgta ccacggccgc
     9181 cccggagctt cgaccaaacg catcgcaccc cgacagttca acggcaaaat tcaggctctt
     9241 gttgagcaca cgcggaaatg gatgggcggg atccagcccg atcggcgtga gcaccggcat
     9301 cagttcgcga aaaaaataat cacgcaccca tttcgactgc acttcgttcc agtgattgcg
     9361 ccgcaaaaag cggacgccct gcgcactcag ctgcggcaag atttcccggt tcagcacttc
     9421 atactggcga tcgaccagcc gccgcacgcg cggctcgatt tcatgcagga cctgacgcgc
     9481 gctgcggcca tcgggcgaca gcagggaggg cgtaagcgca gcgcgttcct tgagtccggc
     9541 cacgcgcacc tcgaagaatt catccagatt gctgctgacg atacagatga atttcagccg
     9601 ttcgagcaac ggcgcagtcg cgtcctgggc ctgcgccagc acgcgttcat tgaatgcaag
     9661 cagactgagt tcgcgattga tgaaatgttc gccagtcaag gtattcatgg tcatgaaaat
     9721 tcaactacaa taagacaccg tcaaaatccg cgaccg
//
LOCUS       DAIANL010000103        14573 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2378665, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000103 DAIANL010000000
VERSION     DAIANL010000103.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 14573)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14573)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14573
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2378665"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2839 a         4935 c         4042 g         2757 t
ORIGIN      
        1 gtctatgcgc aggaactgat tccgggccgg atcggcaccg tctccggcat gttttttggt
       61 ctggcattcg ggctggccgg aatcggcgct gcactgttgg gcatcattgc cgaccactgg
      121 ggcatcgtca cactgtaccg gatctgctca ttcctgcccc tgctcgggct gctggcagtg
      181 ttcctgcccg acctgcaccg ccgcctcacc agctgactgc gccccaagct ggtaggcatg
      241 ccgcagcgca tccagcacgg atgggcgctg cgcggcatcc aggctgtgcg gaagcagggc
      301 gacaagtacg gcttcgagcc gctggctccc ccaccagcgt gcgaatccat ctgcatcgaa
      361 cctgccagca tgcaggaagg cccgtacgtc gtccccgccc gcgccgtcag gcacatcgaa
      421 atgcacctcc gactgctgca tgacctcaaa ctcataggag taacgatcga tgccattggc
      481 atcggccagc agttcgacag catcgaaacc gccaccctga tgcatccagc gctcaaggtc
      541 aatgcacgcc gtcagcgcgt gggtcctccc acgaaaatcg aactgcatgt gcgcccggat
      601 ctgatggatc atggcatgaa ctcctgtcgc aaacactccg tcaacaacgg catcacacgc
      661 tgcacggcgc cacggtgctg ctgactgtag accagcgcgg ccgcccgcat gcgttgcagg
      721 cgtggcgcat cggccagcag gccggctgca acctgcgcaa gttgggcagg atccgccagt
      781 tcggctgccg cgccacaatc aagcgcatcc tggcttgcac gggcgaagtt ccaccggtgc
      841 ggccccagca tgaccggaca accggtcgcc gcagcctcga tcaggttctg tccaccgaac
      901 ggcagcaggc tgccgccaat cagcgcaagg tcggccatgc gataataggc gaacatctcg
      961 cccatgctgt cgccaagcca cacccggatc gatgcggtgt ccggtacagc gactgagcgg
     1021 cgcgcatacg ccagtccacg ggattcaatc agtcgcgcca cggcatcaaa gcgttgtgga
     1081 tggcgcggta ccagcaccag caggatgtcg gcggtggcca ttttctgcag cgcatcgagc
     1141 agcagcgcct cttcaccctc acgcgtactc gcggcaagcc agactgtccg cccggcgcat
     1201 gccgcccgcc agcgctcccc caacgccagt tgaccggctg gcggatcaat atcgaacttg
     1261 aggttgcctg tcacgctgac ccgctgcgca cccagtgcgc gcaaccgctc ggcatccgct
     1321 ccggtttgcg cagcaaccgc acacaacggg gccagcgtgg cgcgcgccaa tccggccatc
     1381 agccgatagc gtcgcgcgga acgttcggac atccgggcat tgaccagcac gagtggcacg
     1441 ccggcgctgc ggcaggcagc aaacaggttg ggccagattt cggtttccat gaccaatccc
     1501 aacgccggac ggaaatggcg cacaaagcgt cgcgtggcga acagctcatc aaatggcacc
     1561 cacgcctgct gcacctgctc accaaacaac tcccgcccgg tcatgaagcc cgttggggta
     1621 ccatgggtca gcagcagccg gtagcccggc cattgcgtac gcaacgccgc caccagcgga
     1681 atacaggccc gggtctcgcc caccgatacc gcatgcagcc agatcgtcgg cattggtgcg
     1741 gccgcggcat ggcagccgaa gcgctcgccc agacgcatcc agtaaccgcg ctggcgcaca
     1801 ccccgccatg ccaggcgcag cacgaacagc ggcagcaaca accagccagc cacgacatac
     1861 aggatgcgtg ccatcacaag cgccgccgct ggtcacccag gacgaacccg agcagcggtt
     1921 cgtccagacc acgcaccagc gccatgacct tgaacaactc tcccatctcg gccggactca
     1981 gcagtttctg caccgctgcc gcgtgcggca gatatcgggc aatctgcagt ggatcctgcg
     2041 cttcgagcag cgcgaggatg ccacagttga gcagaaactg tgcctgtgtg acaaaaccgc
     2101 ccagcttgag tccggcctgc ctgccagcga ccgcaatggc ggtaaaatcg acgtgtgcgg
     2161 tgatatcctg cagcccgggc aaaaagaacg ggtcgtcatg cgcatgctgg cggtagtggc
     2221 acatcaacgt gccgtgctcg cgctgcggat gataatactc ccgctcgccg aaaccatagt
     2281 cgaccagcag caacgcaccg cgctgcagcg cctgactcac cgtgctcacc caggcctgcg
     2341 cctgcaaccc gatttcactg atggttcctg atgccaccgg cagcgcaccg gcctgctccc
     2401 gcaacacgcc ctgcacctga ttaccgtcct tccagtccag tcttccgccg ctacaggtca
     2461 cgccgcgttc gtacacgcca tcggcggacc agcgcagcac gtgtaccggc agcgcatcga
     2521 gcagttcatt ggccaccacg cagccttcga tgcgttccgg caaccgttcg agccagacga
     2581 cgcgttcggc gagcgcaccg gcacgttcat gcagcaacgt ttgctgccgt tctcgcaagt
     2641 cagcgctcac ctcaagaatg aaatagtgcc ggggcagaca atccagtgtc tccagcgcca
     2701 gcagcagatc gcatgccagc gcaccactgc cagcaccggc ttcgatcaca tcacctccgg
     2761 ccagccgcag cacttgcgcc acctgctgcg ccagcgcccg accaaatagc ggcgatatct
     2821 ccggcgcagt gacgaaatcc ccgccgacgc caaattttac cgatccgccg ctgtagtacc
     2881 ccagcccggg ttcatacagc accagatcca tgtaacgtgc gaaatcgatc cagccgccag
     2941 cggcttcgat ctgggcacag atgctctgtt caagtatccg gcttgctgtc aatgctgttg
     3001 catcgggttc gggtagagac atgcgcgagg tcaaaggtta aaatgaagct cagatgaagg
     3061 gattcccatg caaggcaaaa ccgcattgat caccggcggc tcgcgccgtg tcggcgcagc
     3121 gatttcgaga cggctgcatg ccgctggcgc caacctgatg atacattatc gcagcgccgt
     3181 tcaggaagca cgcgccctgc aggccgaact taccgccatc cgtccggatt cggtcaccct
     3241 gatccaggct gacctgcatg aaaccgatgg gttgcccaac ctggtgcaca ccacgctcga
     3301 acgctacggc cggctcgatg tactggtgca gaatgcttcc agtttttacg ccacgccaat
     3361 cggcagcatc agtgaacacg actggttcga tctgatcggc accaacctca aggcaccgct
     3421 gttcctggcg caagctgcgg cagccgaact gcgccaccgc cagggctgca tcgtgcatat
     3481 caccgacatc catgccgagc ggccgatgaa agagcatgtc gtctacagca ttgccaaggc
     3541 cggcctggtc ggactcaccc gctcgctggc ccgcgaactg gggccggaag tacgcgtcaa
     3601 cgccgtggcg cccggaccga tcctgtggcc ggagggagaa tccgcactgg acgaagtgtc
     3661 gcgtcagcgc atcatctcgc acaccatcgt caagcatatc ggacacccgg aggacatcgc
     3721 cgaggcggtg ctctatctga ttcgtgccgg cttcgtcaca ggccaggtca tcaacgtgga
     3781 tggcggccgc agcatcaaac tgtaaccgcc gccgtggaca cggctcaatg caactgcggc
     3841 ggggtgcccg ctacttccgg catttccggg tgcaccaggt cgccttctgc atcgggaaac
     3901 agcggtatgc cgcaatcgtc gcaatattcg aggggcaacg tgttgcccag caccacgacc
     3961 ttgccaagat cggacagttc acgctcgatt tgcggtaaca gttcgctttc ttcactttcg
     4021 tcgcccacca gtggccagac gacaccgtgc aggatgcgtg cgtccttgag cagactgaat
     4081 ccgatacggt attcctcaag cgtctggttg gcgcacggcg cgatgatggc gcgcagttcg
     4141 gcaggcagca ccgacagact ttcgcacaga taattcaccg ccgcacgcaa cgaataggga
     4201 cgcgcttcac gttccagctt gcgccaggcg ctgaaaaaag cgtccggcag cagcacccgt
     4261 acgtggctgc cgggcatcac ggccgaaaac agtgccgtgg cctcggtgcg ccagacttcc
     4321 tccacttcgt cacgcgtggt gtcggcctgt tgccagcgga acagcggcgc atgcttgcca
     4381 agccgcgcac aggcaaacac gtagcgtacg tcggcgacaa attcgcccga ctgcggcaaa
     4441 cgctcggcat cgatgggcag atcctgcccc gtttcagtca gcgcggccag ttgcacgagc
     4501 aactggcgtg tctcgacaaa ccctcgcggc aactgatccg ggctgtagat atggtcggca
     4561 atcgacagtt gcacgttttc ggcaaacagg tgtcgttgca gcaactcgcg cagcttcagc
     4621 aggcgctggc ggccgattgc tccggacaga atgctgtaac gcgaccaggc cagcagcgga
     4681 atcgccacca gcacagcgct gctgtctgcg gcttctgtca atgtttcgga gtaaccttcg
     4741 accagttcgg acaacaagtc gcacgccggt ggattgtctt cccagagaaa gtccagaacc
     4801 tgctccagcg tttcgttctg tttcttctgg aacaacgaca cgaccagagc caggatctgg
     4861 ctgcgccagc cctcgcgctc gatctggctg cctgccgcct gcatcgaggc ggccagacgc
     4921 gccaaacggc ttgcatcgcg actgaggccg gcagacggcg ttccccgttt catcgcgccc
     4981 gccatctcaa ctcagcttgc cgtgacagtg cttgtatttt tttcccgaac cgcacgggca
     5041 cggatcgttg cgccccaccg acatgcccgg tgcagcgcgt ggaaccgcca gcgcagcaat
     5101 cgctgcatca tcgctcgcca ggctcgcact gaaatccgcg tgatgatact gcagattgga
     5161 catgggcagc ggagtgtcga tttcctccac ctgttcagcc gagtgcagtt ccaccgtcat
     5221 cagcacctgg gtcacctcgc gcttgatggt ctcgagcatc atggaaaaca gttcgaatgc
     5281 ctcgcgcttg tactcctgtt tcggattttt ctgtgcgtaa ccgcgcagat gaatgccttg
     5341 acgcagatgg tcgagcgcag ccaggtgttc gcgccagtaa ttgtcgatac tttgcagcat
     5401 gatggcacgc tcgaagtgac gcatggcctc ttcaccggcc tgtgccgcac gctgccggca
     5461 ggccgcgtcg ccggcttgca cgatgcgcgt ccgcaacccg ttctcatcga gccggttgtc
     5521 ctcctgcagc cagcgcgcaa ccggcaactc cagcgcaaat tcgctgtgca gcgctcgttc
     5581 gagcccggca atgtcccatt cttcttccat gctgccaggc acgatatatt cggccatgat
     5641 gccggcaagc acgtccgcac gcatcgcctc cacggtctcg gacacgtcct ggctttcgag
     5701 caactcgttg cgctgctggt aaatgacctt gcgctggtca ttggacacgt catcgtattc
     5761 gagcaactgt ttgcgcatgt cgaaattacg tgcctcgacc ttgcgctggg cattttcgat
     5821 ggcgcgcgtc acccaggggt gttcgatggc ctcgccttct ggcattttga gccggtccat
     5881 gatcgccccg acgcgatcgg cggcgaaaat gcgcagcagc gagtcttcca gcgacaggta
     5941 gaaccggctg gatcccggat cgccctggcg gccggaacgg ccgcgcaact ggttgtccac
     6001 gcggcgggat tcatgccgtt cggtaccgat gatgtgtaaa ccgcctgcag ccagcacctg
     6061 ctcatgcacc tgctgccagc gctttttcag ctcgtcggtc tgaaactgtt tctgcgtatc
     6121 attgagtgcc gcattggtac gaatggcctc aagttcgcgt tcgatgctgc cacccagcac
     6181 gatgtcggtt ccacggcccg ccatattggt ggcaatcgta atggctccgg gcttgcccgc
     6241 ctgggcgata atctctgcct cgcgcgcatg ctgcttggcg ttgagcacct cgtggggcag
     6301 ttttgcctgg cgcagcagcc cggacaggta ttcgttgttc tcgatcgagg tcgtaccgac
     6361 cagcacgggt tgcccgcgct tttggcagtc ggcgacgtcg cgcaatatgg cctggtattt
     6421 ttcctgcgca ctgcggaaca cctgatccat gtaatcggaa cggatcatcg ggcgatgcgt
     6481 cggaatcacc acggtttcca ggccatagat ctgctggaac tcgtaggctt cggtgtcggc
     6541 agttccagtc attccgccca gcttggcata catgcgaaag tagttctgga aggtgatcga
     6601 tgccagcgtc tggttttcct tcttgatcgg cacgtcttcc ttggcctcga ccgcctggtg
     6661 caatccctcg gaccagcgcc ggccggacat gaggcgtccg gtgaattcat cgacgatgat
     6721 cacctcgtcg ttctgcacca cgtaatgctg atcgcggtgg tacagcgcat gggccttgag
     6781 cgcggcatgc acatggtgca tgaggccgat gttggctggt tcgtacaggc tcgaaccttc
     6841 cggcagcaac ccggcctcgc ccagcagctg ctcggcgcgt tcatgaccgg actcggacag
     6901 cagcacgctg tgcgcctttt cgtcaaccca gtaatcgccc tcgccgtctt ccgtctgctg
     6961 ccgcgtcaac ccggggatga tgcggttgac tccgatatac agctcggtac tgtcttctgc
     7021 ctggccagaa atgatcagtg gcgtgcgggc ttcgtcgatg agaatggaat ccacttcgtc
     7081 cacgatggcg aaattcagcc cgcgctgaac cttttccgcc atctgataca ccatgttgtc
     7141 acgcagatag tcgaaaccga attcgttgtt ggtgccatag gtgatatcgg cagcataggc
     7201 cgcctgtttt tcttcatgcg ccatctgcgc aaggttgacg cccaccgtaa ggccgagaaa
     7261 gccatgcagg cgtcccatgg cggccgcatc acggctggcc aggtaatcgt tgaccgtcac
     7321 cacatgcacg ccacgcccgg acaatgcatt gaggtaggac ggcagtgtcg ccatcagcgt
     7381 cttgccttca ccggtgcgca tttcggcaat tttgcctgca tgcagcacca tgccgccgat
     7441 cagctgcaca tcgtaatggc gctgcccaag cacgcggcgg ccggcttcgc gtacggtggc
     7501 aaacgcctcg ggcagcaatt gatccagact ttctcccgcc gcatggcgtg ccttgaactc
     7561 atcggttttg gcgcgcaact gtgcatcgct caaggcctgc atggcgggct cgagtgcatt
     7621 gatggcctgc accctggacc gatactgctt gaccagccgc tcattgcggc tgccgaaaat
     7681 ctttttcaat attgaagaaa tcatgacgcc cagccaattg gaatggtatg ctgccatcgc
     7741 gcgatgcttt cgccgcgccg ccccggcgca gcgcacacgg taacggcgca tgttaccata
     7801 tccaaccctt cccccgaagc gttgcatgag aaagtttgcc aatacgcccc ccctggctgc
     7861 cgaatgcttt gccaccgcca gcggcctgga cgcgctacga cagggcgccc gcgctgtacg
     7921 tcatctcgaa ggcatctggc acgccatcgc accagcgcca ctcgcacgcg ccagccaccc
     7981 cggcctgcca gaagatggcg tgctgccgat cttttgcgac aacggcgcgg tggctgcccg
     8041 gctgcgccag atcgcccccc ggctcgaaca gcagctgtca cagcgcacgc gcttcgacct
     8101 gcgcattgca gtacgcgtac gcagcaagcc gttaccacaa cccgccccgg cagcaccgcc
     8161 ccggcaggtg tccgcacaag cccagtcccg cctcaaacag ttgcacatcg gactcgaaca
     8221 ggggcgtttg cgcgacgcgc tcacccggtt gctgcagcat ctgcccgaac cggatcgagc
     8281 ggactgaata catatcagtg tcggaaatcg ccttccggcc ctgtttcgcc gccacttgat
     8341 ctggaacaga ctcgaactgt cgcctccaag cacgagcgcc ttgcgctcga tgcgccacat
     8401 cgacgcgacg accggcctgg gcgttctacg ctgatgcatt accggcgcat cggacggatc
     8461 gagccgattc cgcgcccgaa cagcgcgcat cggcaaccct ggcgaggaat cagggaccct
     8521 ggcggaagtt ggtggcaccg cttgcgaacc tgccatcgcc ggcctgacct gcgattgtca
     8581 cgtacatatt gatgcggtgt tgaatgtggg tttgtctcgt gccgcgaaat cgtcgaaacc
     8641 atcattccgg cgtgtgcatg cgcaggatct ccggctgcac gaatgaattg tttgccgcca
     8701 gagcggttcg caaatcacgg tgcatgaaca cacactgcca gccgcagcgg gcgtgacaga
     8761 cagacccggt tgacgcgggt ggcaaacacg gtgcgcctgg ggacgcggtg attcatcgcg
     8821 tccctagcag gccgttgaaa aaggtagcga ggacgcccgg atgcaaggcg cgagaaatgc
     8881 aacgacgaga catatcaaat cgataggcgt gggagtgagt atcgcagcaa cgccgcagac
     8941 gggtgccgca gtactttttc aacggtctgc tagaccagac gctccagccc cagcaatacg
     9001 ccgaacacaa tgaacagcac cagtcccgcc ttgagaatgt cgaggatcaa ccgcagatag
     9061 cgccggtcct gggtaacgaa atacagcgtg agcgacatgg ccagtgcaat acctgccagc
     9121 gcgagcagca ggcgcactac aaacatcagg ccggccctgc cgccatgccc aggcggcgca
     9181 cacctgccgg cgcatgcatt tcgtcatcga actgcacgaa ctcccacgcg gtcgcgtcgc
     9241 tgagcagggt acgcaataaa cggttgttga gcgcatgacc cgacttgtgc gcctgaaacg
     9301 agccgatcaa cggatgaccg ataatgtaca gatcgccgat ggcgtcaagc accttgtgct
     9361 tgacgaactc atcctcataa cgcaatccat cgctgttcaa cacgcggtat tcgtccatga
     9421 cgatggcatt gtcgaggctg ccgcccagcg ccaggccatt ggaacgcatc cattcgactt
     9481 cctgcatgaa gccgaaagta cgcgcccggc tgatttcttt cacataagaa gtggtggcga
     9541 aatcgatacg aatcgcctgt ccggttttct cgaacaccgg atgacgaaaa tcgatggtga
     9601 actcgacacg aaagccatcg tatggctcga agcgcacccg cttgtcgcct tcgaccactt
     9661 ctacggcatg gcggatgcga atgaagcgcc gcggcatttc ctgcgccacg attccggccg
     9721 actgcagcaa gaaaacgaaa gcggaagccg agccatccat gatcggtact tccgggccgt
     9781 ccagatcgac atacagatta tccaccccga gaccggccag tgcagacatc aggtgttcaa
     9841 ccgtcgccac cacggcgtcg ccgttgacca gcgtcgagca cagccgcgta tcgccgacgt
     9901 ggtcggccgc cgccacgatg tcaaccggct gcgacagatc gacgcggcgg aacacgatgc
     9961 cagtgttggg cgctgccgga cgcaacatca tgttgacctt ggtcccggtg tgcaatccca
    10021 cgcctgtcgc gcgtatgctg ttcttgagtg tatgttgccg tatcatccct gccattttcc
    10081 agaacgcaac ccgtacgcat cactggcaaa tcccatgccg aagcgtcgag cctgccttca
    10141 accccttaac catcagggaa attatagcgc gaaacaccgc caacgtcaga ctgtatcgtc
    10201 tgacgcaggt tcaacagggt gccggcaagg caccgtctgt cgcttttccg ttacacactt
    10261 gcgcagcgcg accacctgcg cgtcccgcat tacgtcacgt ggtgcacccg attccaccct
    10321 gcggatgggc gcgaccgccc gtcgcgctca gtcagcctgc ttgcgcagaa aagccggaat
    10381 atccaggtat tccatgcccg actgcttgag cgcttccacc tgggcttcgc gccggttgct
    10441 gcgaaacacc gtgggttcat tatcggccgc cggatgctcc atctgcacgg ttgcgtagtc
    10501 atcagtgccg gtcttgacga tcaccggctt ggcctgctgg cgcgccaccg ggctgcccag
    10561 ccccgtcgcc acgatagtca cgcgcagttc gtcgccgata ttctcatcaa acaccgaacc
    10621 agcgatcacg gtggcttctt ccgccatgaa atcacgaatg acgttcatga cttcgaggta
    10681 ttccttcatc ttcatgctgg atgaagccgt cacgttgacc agcacgccgc gggctcccgc
    10741 cagattcacg tcctcgagca gcgggctggc aacagcctgc tgagccgcca gccgtgcacg
    10801 atccgcgccg gtggccgccg ccgatcccat catcgccata cccatttccg acatgaccgt
    10861 acgcacgtcg gcaaagtcga cgttaaccag ccccgtgcag ttgatcactt cggcgattcc
    10921 ggccacggcg ccatgcaaca cgttgttggc cgcgcggaat gcatcgagca tcgaaacatc
    10981 atcgcccagc acgtccatca gcttctcgtt tggaatcacg atcagcgagt cgacgtgacg
    11041 ggtcagtgcc tcgatgccag cgttggcgag ccgcacacgc ttgccttcaa actggaacgg
    11101 cttggtcaca acggccaccg tcaggatgcc catttccttg gccacttcag ccaccaccgg
    11161 ggctgcgccc gtgccggtgc cgccgcccat gcccgcagtg atgaacaaca tgtcagcacc
    11221 gtcgatcagt tcggcaatgc gctcgcggtc ttccagcgca gcttcgcggc cgacttccgg
    11281 attggcgccg gcaccgagac cgcgcgtcac gttgctgccc agttgcagct ggattttcgc
    11341 cttgttgcgc tgcagtgcct gggcatcggt gttgacacaa atgaactcca ccgtatcaac
    11401 accgctctcg atcatgtgat cgacggcatt gccgccacat ccacctacgc cgatcacctt
    11461 gatgacggct tcctgtttgg tatccaccat ttcaaacatc atcgctttct cctctcgtta
    11521 ctgcttgaat ttaaaccgac cctggttcag aagttcctga accacccttt catccgttcc
    11581 atgatctgac gcatcgacgt gccgcccagc ttgccgccgg gccggcggtc ctgctcgtgc
    11641 aacccggcca gcaacaaccc cactccggtt gcataacgtg ggttatgcat catgtccgac
    11701 aacccgcctg catactgcgg cacaccaagc cgaaccggca tgtgcagcac ttcctcgccc
    11761 agttcgacca tgccctgcat cgccgccgac ccgccagtaa tgacgatgcc ggagcgcatc
    11821 agctcttcga aaccgctgcg gcgcaattcc gcctgcacca tttcatacag ctccagtacg
    11881 cgcggttcga tcacctcagc cagcaactgg cgcgacattt gccgcggccc tcgttcgccc
    11941 acgctgggaa tctcgagcat gtcatgcacc cctgccaact ggcgcagtgc acacccatgc
    12001 gcgatcttga tgtcttctgc atccttgagc ggtgtgcgca gcgcaagtgc gatgtcattg
    12061 gtgatctggt cgcccgcaac cggaatcacc gccgtatgcc ggatggcacc ctgggtgaac
    12121 accgcgatgt cggtcgtgcc gccaccaatg tccacaacag cgacgccaag atccttctcg
    12181 tcttcggtca gcaccgccat ggcggacgcc agtggctgca acaccaggtc acgcacttcg
    12241 agtccgcaac ggcgtacgca tttgatgatg ttctgcgccg ccgacaccgc accggtgaca
    12301 atatgtacct tgacctcgag acgcacgccg ctcatgccta tcggttcgcg cacgtcttcc
    12361 tgaccatcga ccagatattc ttgcggcaag atgtgcagga tctgttgatc tgtcgggata
    12421 ttgacggcct tggccgtttc cagcacccgg ttcacatcca gctcggtcac ttccttttca
    12481 cggatcggta ccatgccatg cgaattgaaa ctgcggatat ggctgccggc gatgccggca
    12541 tacacttcac cgatcttgca gtcagccatc agttccgcct cttcgagcgc tcgctggatc
    12601 gcgttgacgg tggattcgat attgactacc acccccttct tcagcccgcg cgaggggtgg
    12661 ctgcccatgc ccaccacatc cagccgcccg tccggcatat gttgcgccac gatggcgatc
    12721 accttcgaag tgccgatatc gagcccgacc accagttccc gattgtcccg ttgcctgctc
    12781 atgatctgtg tccctgtctg gttccttcat gttcgtgcgg acccggctgc attgccacct
    12841 gcacggcaaa cccctgcggg taacgcagat ccacccgcac tggctgtccg cccaactggg
    12901 ccagcatggc cggttcgagc ccgacccagc gctgcagccg ctgctgcgcc tgatcacgac
    12961 cgagctcgat gacccaaccg ttgtccagcg tcacactcca gctacgccgg tcgttcagca
    13021 caaccgcaac cggctggcga ccgatggttg ccagcaccgc accaaaggcg cgccatgcct
    13081 gcgccacctc ctggttggtt ccttccggcc cgctcaatcg tggcagacga ccgtcactcg
    13141 ccgccatgaa cacatcaccc tgcacgtcca gcaacgcatc ctcgttccag cgtgccagag
    13201 gctggtgttc ccccagcgtc acgaccaggc catcgggcca gcggcgacgc acatccaccg
    13261 tgcgtaccca aggcaatttg ccaaacccgg cgcgcacctg gtcgagatcg atatcgaaca
    13321 ggccaccgtg tacgtattgc gccgtcacca gccgcgcctg cgccagtgtc acgtggtgca
    13381 tgccgttaac ctccagttgc ttcaggtgaa acactggcaa ccgcgtgaat tccaccccgg
    13441 ccagccaaac cagcccggcc agtgcaacgc cagcggcgta acgccaccag tgcaggggaa
    13501 tgcgccattt actttgcaca ggtttgctcc aggatacgca gcaccagttc atcgaagccg
    13561 atgccgaccg cgcctgccgc catcggcacc aggctgtgtg gcgtcatgcc cggcgaggta
    13621 ttggcttcga gaaaatacgg gtggccatcg cgatccagca tcacgtccag ccgtccccag
    13681 ccacggcaac caagcacgtc aaatgcccgc agggccaact cgccgaccgc tcgttcctgt
    13741 tccggagcca gcccgcacgg caaacggtat tcggtgtcgt cgcgcacata tttggcgtcg
    13801 tagtcgtagt acgctccagc cggcaccaac cggatcatcg gcaacacctg atcgccgaga
    13861 atggcgcagg tatattcccc gccttcgata ctgcgttcgg ccagcaccag ctcgtcatac
    13921 tgcgctgcct gtcgccatgc ctgtgccagt gcgtccacct gggtgacgcg cgacatgccc
    13981 agactggatc cctcgcgtgc cggcttgaca aatatcggca ggccaagccg gtcagccact
    14041 gcctgccaat cgttggtacc gtcgagcaac acacaatcgg gtgtcggtat gccggttgcc
    14101 tgccagatca gtttggtacg ccatttgtcc atcgccaatg ccgaggccag cacaccgcta
    14161 ccggtataag ggatgcccag ccattccagc acggcctgaa tggttccatc ctcgccatga
    14221 cgtccatgca acgcgatcac tacgcggtca aatttcctgg cgatgagcgc actgatatcc
    14281 tgctcggccg gatcaaacgc ctgcgcatcg acgcctgcac gcagcagcgc cgtcagcacc
    14341 cgatcaccac tgaccagcga cacttcgcgt tcggccgaac ggccacccat caacaccccc
    14401 accttgccaa accgcttcat gcggcctcct ccaatcgttc tccgatgatg cgtacttcac
    14461 acaacagccg cacgccagtg cgctcttcca ccacctgccc cacatgctcg atcagacgct
    14521 caatatcgct tgcgctggcg ccacccgtgt tgacgatgaa attggcatgc ttg
//
LOCUS       DAIANL010000104        10270 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_336137, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000104 DAIANL010000000
VERSION     DAIANL010000104.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10270)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10270
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336137"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1965 a         2823 c         3465 g         2017 t
ORIGIN      
        1 cgccgacaag gtcagcatca acaccacggc ggtggtcaat ccgggagtgg tgtctgaagc
       61 cgccggccgt ttcggttcgc aatgcattgt cgtggcgatc gatgccaaag agaatggccg
      121 gggcggctgg gaggtgtata ctcatggcgg ccgcaatgcg acagggctgg atgcagtggc
      181 gtgggccgaa cacatggaaa gcatgggcgc aggcgagatc ctgctgacca gcatcgatcg
      241 tgacggcaca cgcaatggct tcaatctgcc gctgacgcgc gctgtggcag atgcggtcgg
      301 cattccggtc atcgccagcg gaggcgtggg taacctgcag caccttgcgg atggaattct
      361 gcaggggcac gccgacgccg tattggcggc cagcattttt cactttggcg aatacagcgt
      421 gcaggacgcc aaggcatggt tacaacaaca aggcatcgag gtcaggacag ggtgagcgtt
      481 gcggataacc agtggttgaa caaggtcaac tggtctgaga gcgggctggt ggcggtcgtc
      541 gtacaggatg acctcagttc ggtggtgctg atgatggcct ggatgaaccg ggaggcgctg
      601 ttgcgtaccg tggaaaccgg agaagcggtg tactggtcgc gctcgcgcaa gaaactgtgg
      661 cgcaaaggcg agcaatccgg gcatgtgcag aaggtgcgtg acatccgtct cgactgcgac
      721 ggtgacgcga ttctgctcag ggtggaacag ttgggcggca ttgcatgtca taccggacgt
      781 cgggactgtt tctttcagcg gctggacgac cgtcagtggg tgacggtcga gccggtgctc
      841 aaggaccctg cggagatcta tcatgaatga tgtgctcgac cgcctggcgc aaacgcttga
      901 gtcgcgcaag ggcgcgagtc ccggcagttc atatgtcgcc agtctgtacc acaagggcct
      961 ggatgcgatc ctgaagaaaa ttgccgaaga agctgccgaa accatcatgg cggccaagga
     1021 cggtgaacgt gacagaatcg tgtacgagac cgcagacctg tggtttcata cgctggtgct
     1081 gctggccgag cgcggcctgg ggccgcagga cgtactgaac gaactggcgc gacgcgaagg
     1141 tgtctccggc catgaggaaa agcgatcacg caaggatgcc gcatgagcga gtgcatattc
     1201 tgtcgcatcg tggcaggcga gcttcccagt catgcggtgt acgaggatga tgagctgctg
     1261 gtgttcaacg acatccatcc cgtggcgccg attcactggc tgatcattcc gaaggcgcat
     1321 gtcgagtcgc tggcgcattg tggcccggag catgaagcgt tgctgggccg catgttgctg
     1381 ctgggcgcga ggctggcgcg cgagcacggg ctgacggacg gattccgcac cgccatccat
     1441 accggccggg ccggcgggca ggtggtgttt catctgcatg tgcatatcgt cggcggcaac
     1501 gagtcgaaga caccgattgg ctgagggttt cccctgcccc ggggaatttc aaggagaacg
     1561 acatgggcag tttgagtatt tggcattggt tgatcgtgct ggtgattgta ctggcggttt
     1621 ttggcaccaa gaagctgcgc aatatcggca gcgatctcgg cggcgcggtg aagaatttca
     1681 aggacgccat gcaggaaggc gaacgcgaaa aactggagaa ccacagcacc ggccatacgc
     1741 tggaaggtga agtcaaggac aagaccaaga ctcccgtctg agccgccgca tgtttgatat
     1801 cggattttcc gaactgatgc tgattgccgt ggtcgccctg gtggtgatcg ggccggagcg
     1861 cctgcccaaa gtggcgcgca ccgccggatt gctgttcggg cgtttccagc gctacatcgg
     1921 caacgttcgc gccgatatca cgcgcgagtt gcaactggaa gaagtccagc gtgcaagcga
     1981 agcgctgcgt gaagaaatgc agcgcgctgc acaggatgtg catgccggaa ttgaagcgtc
     2041 ggctgcagtt gcgcgcgagg gcatacagac cccgccgccg gccacgagcg aagggctccc
     2101 ggggcaggcc accccgtccg tcgctgccgc gggcgggtcg gcgacggtcg aaccggccgt
     2161 ggccgaaccc gcaacgcttg ccgacgccgt ggccgcagcc gtgcgccagg ccccacctgc
     2221 aggcgaagcc gccatgcctt cgcacgcgac ggccggaacc ccgacagccg cgccagccaa
     2281 agccgagccg tccgcccccg tcgcacaccc ggcttcccct gcggaggcaa tgcagggcga
     2341 actgactttt gatacgcccc tgtcgccgca caggccggaa tgaacatgga agaattgcag
     2401 gaaaacttta tctcgcacct gatggagttg cgtgaacggc tgttgcgcgc cattctggcc
     2461 gtggtgctga tcttctttgg tctgttccac tggtcgaacc agatctatca cctgctggcg
     2521 cggcctctgc tgcgcacctt gcccaaaggg gggcaactga ttgccaccga agtgaccgca
     2581 ccgtttttcg tgccgctcaa ggtcaccatg ctcaccgcat tcctgctggc gctgccctac
     2641 atcctctacc agatctggtc gttcattgcc cccggtctgt atgcgcatga aaaacggctg
     2701 gcactgccgt tgctggtggc cagtgtgctg ctgttctatt gcggaatggc gtttgcctat
     2761 ttcattgtgt ttccggtggt attcggtttc atcagcggcg tgacgccggc cggggtggcc
     2821 atgatgaccg atatcagcaa gtatctggat ttcgtcatga cgctgttcct ggcctttggc
     2881 gtgaccttcg aagtgccggt ggtggtcgtg ctgctggtca gggtgggcat cgtcagcgta
     2941 gccaagctgc gagagattcg cccctacatg atcgtcgcct cgttcgtcat tggcgcgatc
     3001 ttcaccccgc cggacgtgct gtcgcaaacc atgctcgccg tgccgttgtg gctgctgtat
     3061 gaggccggca tccttgtggc cagcatgtcg ggcaagcccg cagctgccag cgacgcggaa
     3121 gagaccgaag aggaggccgc tcaggccgac tgacaggggc aggttcgccc gagtcgggca
     3181 cgcgcgcgtg ttttgtcagg acaactgcgc cctgggcacc ggtgcggcac ctgactgcat
     3241 tgcgcagggc cacccgggct cacgatgtgt gcaaaccgga catcgggtac gggcagggtg
     3301 cgctgacccg catgcggtcc gttgccggat gcggactcta tcaacgcaat agggcgtcgc
     3361 cggaagtcag gacgactttt cctcatggta ttcatcatcc acatttactg accacagggc
     3421 cgacttgtcg cggttgccgc tgatgatgtg ctcggcggca agtttcgccg tcagcatgga
     3481 atggtcctga ttattgtagc ggtgcatgcc attgcggccg atcaggaaca gattggcgat
     3541 cggatcgaga tgctggcgga tgtcatcgaa gcgggcatag ctgccgaagt aggccgggta
     3601 ggccttgggc atacggatga ccacgctgtc gagcacggca tcggcgtcgg gctcgatcag
     3661 accgatgtgc gccagctcgg tgcggccgag cgccttgagc tgctcgtcgc tgtgctgcca
     3721 cagggcatcg ccttcattgc agaagtattc cagtcccagc cagatggtgt ccggatcgtt
     3781 caccatggcc gggctccagt tgttgaacag ctgcagccgg ccaacgcgga catcgcgttc
     3841 ctggatgtag atccagttgt ccggcggcat gtgatttgcc agagcagagc gtgattgcgg
     3901 gttggctttc atggcgcgca ccagcaggcc gaccgtgatg aaatcgcggt agggcagggt
     3961 ggcggccagt tgcctcactt ccagcggcat ggcaccgccc agggccgcaa ccaggtcctt
     4021 gaccggcatg ctggagcaga cgaaatcggc tgcaaatacc acgcgctcgc ccgtggcgag
     4081 gttttcggct tccacggcgg tgatgcgctg accgtcgcac tgcaggctgc acaccttgtg
     4141 gcgcaaatgc aggctgccgc cggcagcggt gaccttgtct gcgactgttt cccacatctg
     4201 gccagggccg tatttcggga agagaaagcg ctcgatgagc gaggtgttta cttttttctg
     4261 tccggcgtcg ttgcgaaatg gcagcatgcg gcccagcgca tgcagcagtg cgctgcggat
     4321 gctcagaccc ttgatgcgtt gcgcccccca ttcggcgctg atccggtcgc agggcacgcc
     4381 ccagaccttt tcggtgtaat ccttgaaaaa tgtcaggtat aactcacgac cgaagcggtt
     4441 gatgagaaaa tcttccagcg tggtttccgg gcggcgtggc tggaaatgaa cgcggacgta
     4501 gctcatgccg atgcggatca tgcgcatcag ccccagattg gcgaccgtgg ccgcactcag
     4561 cttgagcgga taatcaaaga atttgcgcag aaaataaatg cgcgacagcc ggttgcgcac
     4621 gagaaacacc cgctcgctgt ccgccgtggc ggagctggat tcgacgtcgc gtgactggtt
     4681 atggtagtgg atggtggttt ttcccgcgcc atcattgtcc tggatgggca ggatgtcctt
     4741 ccaccagttc atcacccagt ccgacttgga aaaaaaacgg tgaccgccga tgtcgatgcg
     4801 gttgcctttg tagttgaccg tgcgcgagat gcccccgacg caatcgtcgg cttcgagcac
     4861 aatgggtacg atgtcgctgt gccgcagcaa ttccagggct gcggtcagcc cggcagggcc
     4921 tgcgccgatg atgatggcct gtctgcgtgt cgaaagtccc atggtcagcc tgtcagtatg
     4981 aggaaagatg cgcaagacgg gatcataata cgaagcggtc aaatccgtga cggatattga
     5041 tcatgcgtcg ctagtcattg cgggtggcgt tcgggacagc ccggccgctc ccggccgtgc
     5101 agacagagcg gtgcggtcat gcacggccat gcggatgacg gcgactggcg atcagtcggg
     5161 cgcctgatcg gggtgctggt ggcttgcccg cgccagccgg tattgtggcc agcgcgtgaa
     5221 cagcagcgca cgccgcaggc cgaaattggc gatcagcgtg aaccccgtgg tgatgcattt
     5281 cgcccacaac acggccaacc ccagttggcc gaccagcagc gccatcaggc ccagcgacag
     5341 tcccatcgtg atgatgccca ccagcaggaa cagaccgaac tcgatgccgt gctgctgcag
     5401 gctgcggtag cggaataccc agcgcacgga taactggtag ttgatggcga ttccgaccat
     5461 gaacgcgatc agtgtggcta gcaggtagtg catgccaaag taattgtgca gcagcgccag
     5521 tgtattgatg tcgccggcaa atgcaatgcc gccaaccagc agataacgca ggaactcggc
     5581 ctgcatggaa tccttggtgg agttacgcat gaggctctcc ttgagggggt gtgattgacg
     5641 gctgtgcatg ttcggcgttc agcaccgcac gacacgcata ggcatagtaa tggtaaccgg
     5701 tgttggggtc actgaacgga tattcgctgg ccagtcccgg aatggggtag cgcaagatca
     5761 gcgtatgcaa caccggatcg gcgcagacat aggcggcatc ggccagcgga tgcggtttga
     5821 tgtcggagga cgtactgttg ctgagggcat aggaccagtt gctgtcggga aatccggccg
     5881 ccttgagtcg ccgcatgcgg cgcatgccct cgaatgccgt atccggagta aagatcgtgc
     5941 cagccagttg ctggaacgaa acatagtctg cgcgatgcag ccagaaccac ggcgcgcgaa
     6001 agctgtgttc ccagtagacc actgcgccgg gcgggattcg ttgttgcagt accgcaacga
     6061 tctgctcgcg ctgtggcgta ttgggcagtc gctcggcggt acgcatgtcc cagtgcgtgg
     6121 cggcaaacag cagcatggcc ggtgccgcca gccagagtcc ggatgacagt gcatgacgtc
     6181 gggtgggaca aaatcgccag cacagggcga cagcgatggc gcaccatggc agcagcagcg
     6241 tgatcaggct ccagccggct ggcatctggt cgaacagcgt gtatttcagg tacgacaacc
     6301 ggccatcgag tatcgcccat agcagcgttt gtaacgggaa taaccagaac accagacgca
     6361 gcaggcgtcc ggggtgggcc agggctggca tgcgtgcggc agcgtaccac cagcccaggc
     6421 caagcattgc gtatggggcg gcgccaaccg ggttgcccag cgcaccacac gccagcagca
     6481 gaacggaccg gcggccattg tcgccgctgt tccacagtct gtcggttgcg ggaaaccaga
     6541 gcagcacggc gaacagttgg gcaatccata cgaagcgcac ggtttgcagg ctcagcaaaa
     6601 aactgttgtg cgtcaggtcg gccagggctt ccagtgcgag accggccagc gcaaccagca
     6661 gggcagggga tgcgagatct gtgcgtggat catccaggtt gcggatggca aaggcaagta
     6721 cgatcaggct gtagaggatc agcgagatat tgtcccaagg ccagaggtgg atgaacagcc
     6781 agggcgaacg cagttcttcg tagggacgca gcatgcccgg aagcggttga gtaacatggg
     6841 caaaggggcc gattcccgaa gcgatcaatc cgccagcgag gacgcagcct gcggcgatga
     6901 gcagcacacg catgcgccag gtgcgaatgt ggtgcagggt ggcgacggcc atgaccggca
     6961 accccatgag tggatgtatc agtgcgccga gtaacagact gctccaggcc agaacgtgtt
     7021 tcctgcgcaa cagcagaccc agcccgagca gcgagaacgc ttctgcatac aggcgtggcg
     7081 tgacgaaagg ttcggcataa ccgaaggttt gcgtgtactg cgagcggaac agtgcaaccg
     7141 ccagcagtgt ccatgcccac aaccggcgcg gcaataacgt ggtgaccaga aacagcagtg
     7201 cggccagcca cagtacctgt ccggtcaggg tggcatacat caggccgctg tcgaggccga
     7261 aatggcgaat ccaggccacc agatacgggg taaacagcgt gtagctcgcc tgcgagccat
     7321 agagaaagaa caggtcgttc cggtacagca gggggtgtat cgaatgcagt gcgatcagtg
     7381 cgtacaggct ttcatcgtgc cagatgccga aatacggccg tttgatcatc cagaccgcca
     7441 gcaccagcaa ggcgaaccag gcgccaaagc ggtgtgtcct ggaggaagaa gcgatcgtag
     7501 cgttcatggc ttcctgccgc agaagggcga aatgaaggcc acagacgtgg ccttcgctgt
     7561 gttctccgca gttgcggccg gcttgtggcc gaccgcggcg gaccagagcg gttcagatta
     7621 cgaaccgttc agtttctggc tcacataagc taccgcagat tggttggtca tgccaaccgg
     7681 ggcggcaacc caggacagcg acgaaccgcc accggcagcg ggcggggtgg ccgtgaaggt
     7741 gatgctgctg ttggccggca caccagtacc gatctggctg gcaaaggtgt aggtgatttg
     7801 cccggtggat ccggattggc tgatggtgat ctggtcacca ttggccgggt tggtgatgtt
     7861 gaagccttcg gtggtcagtt gcgcggcagt cgggaaggtg ccgttgctct gatagtcgtt
     7921 ggccatggcc agctcgacgg aggagacgct ggaggcggcg gccgagagct tggagttgat
     7981 cacatagttc tggtactggg gaatggccac ggcggccagg atgccgatga tcgcgaccac
     8041 gatcatcagt tcgatcagcg taaagccttg ttgtttcatt ttgcttctcc ttgaacggaa
     8101 atggggaaca cctgcggtgt cggtcagttt gctgcaaggg gtgtgccact tttttggcgt
     8161 gtgcataaaa attaattttt acgatgcata atcaatataa tacgatctta cccacggatt
     8221 ttgttgcctg cgatgcaggt caatgatcgt ggtcacgccg ttaaacggct gttgccgacc
     8281 ggcacaatgt gacgttcagc gtcagtgtat gcaattgcgc tggcaatgtg tgtgataacg
     8341 gggtctgcag gatgatcggg ccgtacggta caggagccgg tagcggcgct gctgcggcca
     8401 gcccggcacg acggcgtgcg gcggggctgt gttgccgctg attcggggct acaatgtcgc
     8461 agtgttcatg ctgtctggat gtgggtgata tgattgcggt ggtgctggct tatctgttgg
     8521 gttcgttgtc gtttgcggtg ctggtgagcc gtctgtccgg tttgccggac ccgcgcagcc
     8581 acggttcggg caatccgggc gcgaccaaca tgttgcgcac cgggcgcaag ggcgcggcgt
     8641 tgctgacctt gctgggcgat ctggccaagg ggtggctggc ggtggtgtgc gcgcggctgc
     8701 tgtacgaggc cgggtatgcg ccgctgtggg cgatcgacgg tgcggctgtg gcggtgctgc
     8761 tgggccacat gtatccgctg tatttcggat ttcgcggcgg caagggggtg gccactgcgc
     8821 tgggcgtgtt gctggcgcta tcgccgctgc ttggggtggt ctgtgcggtg ctgtggctgc
     8881 tggtgttttc gctgacgcgt atttcgtcgc tgtcggcatt gctcgccgca ctggccgcgc
     8941 cgctggcggg gtggtggctg ctgggcaacc ggcgcgcggt gctggcgttg ggcatgctat
     9001 cggtactgat catctggcgg caccgcgcca atatcgtgcg cctgctctct ggtcaggagg
     9061 gccggtttgc cgggccgaag tgacggtccg gctgccgggc cgggatatcc agtcgaacga
     9121 tgaggcatcg gtatggcctg gtttgaaaag gaaatgaatt atgcgcgcga gcagttgacc
     9181 aacgcttcgc gcgattcgat tgcgcttgcc ggcgaccgtc tggcagaagt ggtgcgcgat
     9241 ggcgcggaga aagtgggctc cgaactgcgc gaagtggtgc agtccaccag ccacgagatc
     9301 gatgccaagc tggacaagat ttctgccgaa ttgcacaacc agcgccagtt caccaaggat
     9361 gatgtacgcg agttggtgga ttatgcggcc gaccggttct cgagcgcgct ggatcagcgc
     9421 atggtggtgg cgcgacagga aatctccgat ctggtgcagg agaaagtgga gtacttcaag
     9481 gtcgaggtcg atcagttctt catccgccgg cagaaagacc tcgcgcgcga gcgacagcgg
     9541 ctgtggtgga atgtggtcat tgccgtaatg acttcgattg cggtgggtgt ggtgtcctgg
     9601 ttctaccagc atgtggcgcg cgggcaactc gatctgttcg gcgtcttccg tatcgtgttc
     9661 ggctcactgt cggggggcta tgtcgtctat ctgctggtgc ggctgtggat acgctggcgc
     9721 agcatggcag agcaccgcaa ggatgcagtg ttcatggcca tgcgctactg gggcgtgctg
     9781 cgaccggaca gcctgttcat gaatgtggcg atgctggtcg tcatgttgtt gctgttgcag
     9841 atggtgctgt ttcccgaatg gctggagcac ttgcctggcg gtcgcgaact gacacgggtg
     9901 ctcaaggaca tgtttccgca tccgcgctgg tagtgcgtgt gcttgcaacc tgcgtccgca
     9961 gccggggtgc ggcaggatgt cgcccccgat tgtgcacggt cagcagagca gtaccgccgg
    10021 gcaggcacgt agcggcaggc cgcggtacgc ctgacggcac gcgaagaccg tgccaatgaa
    10081 cagggaaacc cccggagtga atggtcaggg tttctgactg gtatcatggc tgtcagccgg
    10141 aatacccctt gcatcgaacg ggttggcaag cgcgtcatgg cggagtaggc tgaatacatc
    10201 gctgtcatga caggagaaac gatcatgtat gctcccatgc tggaacgtgc gcgcggcctg
    10261 ccggtgcgcg
//
LOCUS       DAIANL010000105         6364 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_547566, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000105 DAIANL010000000
VERSION     DAIANL010000105.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547566"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1491 a         2128 c         1674 g         1071 t
ORIGIN      
        1 cgattgccta caacaacatt gccggcctca acctgatcta cacgatgaca agcttctgac
       61 ggaaaacata ctcacatggc aacgaacacc agtctctccc tgcaagaata cctggcaatg
      121 tttcgccgcc acgcgctgct gatcgcggcc atcgttctga gcatctcgct gcttggcagc
      181 atcgtggccg ccatcctgcc cagcggctac gaatcctccg ccgtgattct gatcgtgccg
      241 caagacatat cgcaggaact ggtaccttcg gcgttcaacg actacgccga ccaggaaatc
      301 gacatggtgc gccagcgcgt catgacgcgc gacaatctgc tcgacatcat ccgcaaatac
      361 catctgtatg gcgccaacag cacttctccg ggcacacagg aacaggtgga tgcttttctg
      421 agaaacgtat ccatcgacct gatcgacacc aaaaccagaa cccagaccaa tcgcaatgtc
      481 agtccgacca tcgccttccg cgtttctttc cagggcggca atccggcgat cgtcaacaag
      541 gtggacaacg accttgtgac gctgttcctt aatgaaaacg ccaagaccgg cattgcgcgc
      601 gccaccgatg ccaccaactt cctcaaccag gccgccagcg ggctgcaaga ccagctgaac
      661 agagccagcc agcagatcac gcagttcaaa atgcagaatt cggcctcgct gccggagaac
      721 aaggacgtga ccatggccgc ctatcaggcc acggtagcgg cgctgcgcga cctgggacgc
      781 gacgctcagg ccacccagga tcagatcagc aatctggagt tcgagcaaca ggctgcacaa
      841 tccggcatcg gccccaccaa tgaccagacc gacgctgccg accctgccgt gcaactcgaa
      901 cggttgaaga atgaatacac ccggttgtcc gccatttaca acgacaacca cccgactatg
      961 gtcagcctgc gcaacaagat caagaacctg caggattcgc tggccagcaa tcccaaagca
     1021 ggcagcagcg ccactgcggc accaggttcg gatggcgacc tgttgtcact gcagatctcc
     1081 gccaagatca aggccgccaa atcgcaactg gcattgcttg cgcaacagcg gcaagctgcc
     1141 gaagcacatc tgaaccagtt gcagcaccag ctgttgctcg aaccggaagt cgcgcaacag
     1201 ctttccggct tgcagatcaa tttcgacgac ctgaagaaaa aatatgacga aatccgcctc
     1261 aagcagaaca gcgcgcagct caacgccagc ctgcaggaag aaaacaaggc cgagcacttc
     1321 gaactgatcg agccaccggt cgtgcccgac aagccggtca gacccaaccg catcaagatc
     1381 gttgccatgg gcgtgctggc atcgctgatg gcggcggcag gagccgtatt cctgctggag
     1441 aatattggca agaaagtgcg tggcgtcaat aacctgattg cggccaccaa cgtccagccg
     1501 ctggtcatca ttcccgaaat tcgcagcgag aacgaggcac ggcagcaggc acgccgtctg
     1561 cgtaacggta tggccatggt gctggcgggt tttctgctgt tactgctgct ggtgcatgtc
     1621 ttcatcatgc cgcttgatca gctggcacac cgcgtgatgc actattcaac cgttccgatt
     1681 aagggttcct atggcaaatc ttgaatcgtt gatccggaaa atgttcggac agggcgcacc
     1741 gcgggaagcg cgtaatggac cgcccacgcg catgcggctg cacaatccgg taagcatccc
     1801 ccccggcgac aagcaggaat cggatctgca gtccgtacag tacatccaga cacgtgtcac
     1861 cgaactggat ccgcagcacc tgctcgacaa ccgcatcgtc gcgctcaaca agcgcgatcc
     1921 gaacagctac ctgttcgact tcctgcgcac ccaggtgctg cgcaaaatgc aagccaacaa
     1981 ctggcgcagt ctggctatcc tgtcgcccac gccgggcgcc ggcaaaacct tcgtcgccat
     2041 aaacctggcc atcagcatcg cccagcatcc gaacaagagc gccattctgg tcgatatgga
     2101 cctgcgccgc ccgcaggtcg cctaccagct cggcctgccc agcacgcgct cgctgtacga
     2161 ctatctcgga ggcacggcca gcctggcgca ggtcctggtc aacccgggca ttccccgact
     2221 ggtcattgca ccggtctacc agccgatcga aaattcggcc gaactgctgt cgagcaagaa
     2281 ggtgcacaac atgatcaccg atctgcgcca gcgctacgac tcgcgcatcg tcatccttga
     2341 cctgccaccg ctgctgtcgg ccgatgacac catgatcgtg ctgccccagg tggactgcgc
     2401 cctgatggtc atcggcaatg gcaagcacag cgaaagcgaa ctgcaggaca gcatgcgtct
     2461 gctcaatggc gtcaatctgc tcggcacggt catcaaccgg gtcgatgccg cccccgtcat
     2521 cgaaccgtat tgaccccaag gttcgcccaa ccgacctgct ccggacccac acatgaaacc
     2581 tgcccacacc ctgcttgccg gaatttcctc gctggcgctg gccctgatgc tgtctgcctg
     2641 cacctcgcca caagaacgcg ctgcagaaca tctcaggcgt gccgatgccc tgctggcgca
     2701 aggcgacgac agcaaggctg ccatcgaata tcgcaatgcc atacaaagca acaagaacct
     2761 tgcccaggcc tattacgggc tggctgccat cgatgagaaa aagggcgcct ggcaggaaat
     2821 gtacggctat ctcaacaaga ccatcgaact ggacccaaaa aacactgagg cgcggatcaa
     2881 gctgggtacg ctgatggtcg tgtccggcag gctcgatgat gcgctgcaga tcagcaacaa
     2941 gctgatggca gaacacaaga ccaatccaaa agtgctggcc ttccgcgcca cggtcatgct
     3001 caaacgcaat gacagcaagg cggccataaa cttcgccaac caggccatcg ccatacagcc
     3061 tgccaccact gaagcctggg ccgtactggc ttcggagcgg cttgccgccg gtgatcaggc
     3121 aggcgcggtc gctttcctcg acaagggact ggggcacgat ccgaaaaacc tggcgctgct
     3181 gttcatgaaa atccgcgcgc tcgaacagct cggccagctt gcgcaggccg ggcaggtgct
     3241 ggatcaggcg atcgcctact atccgggtga cctgagcctg ctacaggcga aaatggcatt
     3301 tcaggtccgt catggcatga cggcgcaggc cgagactacc ctgcacgaca tgatgctgcg
     3361 caacccggac aatatgcagc tcaagctcga atggatttcc tggctcaatc agtaccacgg
     3421 cgcgcagagc gcaaagcagg cactcaacca gatgatccag gcgcaacccg acaacagtca
     3481 gctgcagttc gccctgtatg acctcgacta cgaactgcat gacctcacca atgcacgcgc
     3541 ggtactggac aacatcgtcg cgcacccggc taccccgcag gacggttaca aggcacaggc
     3601 catgctggct acgctcgaca ccattcaggg ccggacacag gaggcacgca agcgcatcga
     3661 agaggtactg gccgccgaca aggccaacgt tcaggcgctg atcatccgcg ccggccttga
     3721 aatcggtgct cgtcagtacg atcaggccat cagcgatctg cgcacagcaa cccactattc
     3781 gccaaacgcc agccaggcgt ggttcctgct gggttcggtc tacagccaga ccggcaaacc
     3841 cgatctggcc atgcaggact acctgcaggc cgtcaagacc agcaacaatg acccgcgcta
     3901 cagcgtcccc tacgccggct ggctcatgca gcgccatcag ctgcaaccgg ccgagcaggt
     3961 attgctcgac accctgaaac agcagcccga tcaggtcgca gcgctgacac tgctggcaca
     4021 ggtacagatc gcacaggctg actggtccgc tgcggctgca acggccgacc acctgacaaa
     4081 aatcggtgga acagcaccga cggcagaaca gatccaggcc gaaatccagg ccagacaagg
     4141 caactttacc gccgcactgg ctattctcaa ccgggcgttc gcgcagtttc ccgacaacgt
     4201 gcggcttgtc tcggcgatct tcaatacgca cctgatgacg gggaaaccga aggacgcgct
     4261 ggatttcatc acgtcgctgc tcgcaaaaca tccggacagc gccttcatcc acctgctgca
     4321 aggcgaggcg tacgccgcac tcaacgacac gcctcatgcc atcgaagcct ggcaatcatc
     4381 cattgccgaa gcaccgcgtt caccgctggc ttaccagcgg ctggccgacc tgtacctcgt
     4441 gcagaagcaa tacgacctgg cgcaacaaac cgcgcagaac ggactgaaag cggtaccgaa
     4501 cgatttcatg ctacgactga ttgaggctga gatccacgat gcccgacacg aaaccgacca
     4561 ggccatcgca atctatgagc agctgctgca ggaaaaaccc gacagcgatg tcgtcatcaa
     4621 caatcttgcc agcgcgctcg atctgcgcag cgatgccgcc agcctcaacc gcgcctacac
     4681 cctggcgcaa tcgctgcaag gcagcaccct cccctacttc aaggacacct atggctgggc
     4741 aagctacaag gtcggcaaac tggtcgatgc cgcgtcctat gtaggcagtg cggccagcgc
     4801 catgcctgac gaagcactgt tccagtatca tctgggcatg atcgcactgg ccagcggcaa
     4861 ccgcagcgat gcacgcaacg ccctcaccct ggccgccaaa ctgagcaaca ccgatcccgc
     4921 gctgcatgcg caggtattgc aggcccagca aacgctcgcc aaaccggact gaggatgccg
     4981 cgccgacctg acgtcgttcc gggggcggcg ccccggtgcg atgtcatcct gctgcgctac
     5041 cgctccgggc cagacagcgc agctcccagt tccatgcatc ccgcaggatt cccgtcaatt
     5101 cagcgtgttg cggccgccag cccagttcac gcactgcgcg ggcagcgtct gccaccaatc
     5161 gcgccggatc acccggacgg cgttcatcgt ccaccacggt gaccggcttc ccggtcacct
     5221 gctgcgcagc ttcgatgacc tgttgcaccg aaaaccccac gccgttgccc agattgaacg
     5281 catcgctgcg atcgtgtcgc tccatccagt ccagtgccag caggtgcgcc gaacacaagt
     5341 cggccacatg aatgtaatcg cggatacagg tgccatccgg cgtatcgtaa tcgcggccaa
     5401 acacgcgaat atgcggcagc cgccccgaag cgacccttaa aacgagcggg atcagatgcg
     5461 tttccggatc gtggcgctct cccagtcgcc cggcagggtc ggctcccgca gcattgaaat
     5521 agcgcaggca cactgatttc agtccgtaag cccgatcata atcggccagc gcctgttcca
     5581 ccatccactt cgatttgcca taaggattga cgggctgacg cggatgcgcc tcgtcgatgg
     5641 gcacgcattc cggttcgcca aaaatcgccg ccgtggatga aaacacgaaa cgacgcacgt
     5701 cgtggcgcac catgacatcg agcagattca gcgtattgga aaagttgttg cggtaatacc
     5761 ggtcaggcaa ccgcaccgat tcgccgacct ggatccagga agcgaaatgc atgaccgcct
     5821 gggggcgatg gcgcagaaaa accgcttcaa gggccccggt gtccgcaaga tcgccatgga
     5881 cgaattctcc cccggtcacc gcatcgcgaa acccgctcga caggttgtcg aacgtcacca
     5941 cctcgtgccc ttcgtcacac agacgacgca ccatgtgcga accgatataa cccgccccac
     6001 ctacgaccag aaccttcatt accctcaccc tcgcaagaca ttccctgatt gcatgcaatt
     6061 gtacccggaa agcagaatgc ttactgccat gcaatcaatc tgtcgcaacc tgacaccgct
     6121 gatcacggaa attgacaaat ttgctcaccg acatatgctg ttatcaacaa atgctcaacc
     6181 acccctgtga tcacaccctg cgacagcgaa acacctgccg ccccacccat cccgtgacag
     6241 gagacaccga tgaaacccgg aacgctcaaa ctttcagtca ttgcctgcgc catggcgctg
     6301 tatgccggtt ccagcttcgc gctcgattac ggcgatcccg gctggtccag cctgccgacc
     6361 aaaa
//
LOCUS       DAIANL010000106        36822 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_747756, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000106 DAIANL010000000
VERSION     DAIANL010000106.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 36822)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36822)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..36822
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747756"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         7832 a        11746 c        10041 g         7203 t
ORIGIN      
        1 cgatctggcc gaggcccagg gcgccttgcc cgtcagcgcg gtgagttatc cgcacacctg
       61 ccgcgcctgg gacatgtcca cgctgttgat ccggttgcaa catagccgcg ccacactcgg
      121 cccgaatgcc gccccccggt ccgcgctcga tccggcgcaa cggcaaaaaa tgctcgccac
      181 gttcagccac gcggaaaagc agtggtaccc ggcacatctc ggcaagctgc gcaaaaaccc
      241 gcgcatcaag gccaaccggc acctcaatgc agtacatggc ctgcccgcac tatgcaacaa
      301 cgtacgacgc agcatggaag aatctccggg cggttcggca gattcgatgg aaatcaagta
      361 caacgaaatg gtcgatctgc aaatgtacgg attcgtcacc gaatcaacac gcaaccgcca
      421 tcagagcctg cgcgccccgt tgaagatcca ggaaccggtg gaacactggg agagcgagaa
      481 cgaaagtgcc aacggctacc tggtgcgctt tcccgtcggg aacagcaact ggatgcacct
      541 gggcagcctg atcggcgtgc gtcaggaagg gtcgccggac tggaaggcga ctgtggtgcg
      601 ccgcctgatc aagacgcagc agaccacagc aatggccgga ctggaaatca tcgcgcaaca
      661 gccggtgccg atcaccctgc tgccgatcca gtcggatccg caaacgctca atgttgcacc
      721 aagcgccacc ggacagacca tcaaccaggc actcatgacg cggccgttgc acgacaccca
      781 gttcacgctc atcatcgaca gcgtcaatta tgcacgctca cggttataca aattccagat
      841 ggttgccgga caaccattca cctactttcg cctcgggcag atcatcgaaa agggtgatct
      901 gtgggttcac gccgaagcca tggtaatccg cgaggaacat acagacccgg ccggcctgac
      961 cgggtagacg gcatcacgcc gtacgcggtg tgccctgcaa ccggtggata agccggtcga
     1021 ccgcacgttc ggtgaacgat cccgacttga ggatacgtgc ctcaccgctg cggaaacggg
     1081 cagcgaggcg cggcagggta atggtaatgg cgtccagccc ctggctgttg gtgaacaggt
     1141 acaccccgcg caatgaactc acccacgaca accgggctga cttgtgctct ccctcgggcg
     1201 tatcgaattc gacccacatg cctttcttca gcccgcgcgc ctgctcggtg aaggtatcgt
     1261 cggcaagttc atcgacaggt tcggctgaga aatcgaaatg cccggtcacc tcctcgatcg
     1321 cgccaacttc gggcaacgga cgggcggaaa cctcggtcaa cacggaagaa aacgctaccg
     1381 tatccacctc ggacggcttg actgtcaccg tctgctgcag ccccgccttc accgctgcgg
     1441 agtggcatgc aaccagcgcg gagaaaatca cttcacgctg accaggttcg acttcaatgc
     1501 ggttcatgcc ctcacgcaac tgacgcagca gttcaggcag catggccacc agcgccagcc
     1561 ggtcttcggt gcccatcttc ggcgtcacac tccagagcag atcgcgcatg gtttttatcg
     1621 cagcctgcca gtcggcacca cgttcgccat ccatggtata actgtgcatc agcaccttgc
     1681 gccaggtctg gcgtacaaaa tccacgatca gcccgggcag cacctccatg ccacccggaa
     1741 tcagcgtgct gtcggcaagc gtgcgttcta tggcatcctg cgccacggtc tccgccagat
     1801 gcgagcgttc gacagtctcg atcgcctggg cagtcttggc cacggtaacc tgggcctgcg
     1861 cctcatgttc ggcaagaaag cgctgcagat cttgctgcag ggtctcgaaa atcgtcacgt
     1921 catcggcaaa atcattgagg atggtctgga cgatgctgtt catcttggca aacagcgctg
     1981 cattccactc accctcttcg gtccagccaa tcgatgcgct ggccatttcg tccagcaggc
     2041 gccgcgctgg gtgacttttg cgtgaaaaga actgccggtc aagcatggcc accttgagca
     2101 tgggaatctg caaccggcca atcagcgcct tgagcgtagc cggaatctgc tcttcgtcga
     2161 atacgtaatc gaacagcatc gccacgatat cgatcacaaa ctcgtccgtg cgattctccg
     2221 cacgcaccag ccccacatcc cggatctcgc gcaacacgtt cacggcgccg gattgcagcc
     2281 gcgcaggatc gaaattcgcc ggcatcgagc cggtccactg tccacgctgc aactgggtca
     2341 gcgacgaaat cgggctggca tacatgcccg gctgcgcctc gcccgtctcg gcccagccgg
     2401 gactaccgcc caccgatgca taccctccgc cggcagaaat ggccccgatt cctgcgccag
     2461 ccggcgcggg cgcatagcca ccaccaccgg tcatcatctg tcgcgcaagc agttgctgca
     2521 gcaggacgaa cacatccggc gcttcaccgc cagcctcttc tccaccagtc acccctgcac
     2581 cgggtgttga aattcccgca gccgatgccc cgggaacacc cggtgccggc gcccgtgccc
     2641 gcgcagcacg cgtctgacgg ttgtccatac ccaccttgat cgagggcaga atgtcccggc
     2701 cggcaaggaa gcggttcagt tcctggaaaa tcttcggcat caccggcacg acactagcgt
     2761 caaactgctg caacaggatc agcctgatct tgatatttgg atcgagctcg gcgcacgcgt
     2821 cgagaaatgc ctcgcacagc gcctgcggtg caaacggatt gctcccgtag cgcgcatgca
     2881 ggctctgcgc cagcagcgcg atacgctggt ccagagcagt cagctcctca acggcatcgg
     2941 cctgcacacg cgaagccact ttgccgacgg caagcgaaat ctcgaaagcc gtgtcgtcca
     3001 ccagtgacag gctgggaccg ctatcttcga gcgcttccgt ttcgccagca agatggcctt
     3061 cgatcagggc ggtaatgcgc gcggcgatgc ggttgcgcaa ccccgacatc agcgactcgt
     3121 tgcgctggcg caacaaacct tgcgcctgat agtaccagtt gcggttttcc aggatcggag
     3181 agttttcgcc gacagacaac aggctcaact cgatggcgtc gcgctgatcg ttccaggtgt
     3241 gctcaatggc tgcggtcagg cggtcacggc actcgttcag caacgccaac gcctcgcccg
     3301 gcgatacgcc cttgcgggat ttgtcgtact gcgacaggct gactacattt cgctgcgcgc
     3361 tttccatatc gtcctcatcc ggcatattcg catccgatcc tggttccaac ccacgaactc
     3421 tccatccgac acggttgccc aacacccggc cacgccgcgg cgtccctgtg caaggcaacg
     3481 ccgataacgg ttattttagc ttattatgac tggaaagaca gcgcacatca gcgccggatc
     3541 aacatgtgga gcgggtgatg ggaatcgaac ccacgtctag agcttgggaa gctttcgttc
     3601 taccattgaa ctacacccgc tgtggtagaa ttggcaacca atgatacgcc aatgaccggg
     3661 aaatgagaag gtgatcaatc tgacagtcaa tggcacggcc agacagcttg cgccaggctc
     3721 cacgcttgca gacctggtgc ggcaactcga cctcggcgag cgcaggatcg caatcgaacg
     3781 taacggacac atcgtgccgc gcggcgaaca cgcatcgaca ctggtcggcc agggtgacca
     3841 gatagaaatc gtcgttgctg ttggtggcgg ctgacctttc ccggttcccg agagcacatc
     3901 cgaaaggcac aacacaatat ggatcaatgg acgcttgccg gcaagagcta tgactcacgc
     3961 ctgctggttg gaacgggtaa ataccgtgat tttgaacaaa cgcgtgctgc cgtggacgcc
     4021 agcggtgcac agatcgtcac cgtcgctatc cgccgcacca atatcggcca gaacccggac
     4081 gaaccgagcc tgctcgacgc cctgccgcca gagcggtata cgttgctgcc aaacacggcc
     4141 ggatgctaca ccgccgatga agccgtgcgt acgctgcgac tggcgcgcga actgctggat
     4201 ggacacgatc tcgtcaaact cgaagtgctt ggcgatccgc gcacgcttta tcccaacgtg
     4261 acagaaacgc ttgctgcggc gcaaaccttg atccacgagg gcttcaaggt catggtctac
     4321 acttcggacg accccatcct cgcccgccgc ttcgaagaca tggggtgcgt ggcagtcatg
     4381 ccgctcgcct cgctgatcgg ctcgggcatg ggcatcctca acccgtggaa cctgcagatc
     4441 attctgcagg agctcaaggt tccggtgatc gtcgacgccg gcgtcggcac ggccagcgat
     4501 gccgccatcg ccatggaact cggctgtgcc ggcgtgctga tgaacaccgc cattgccgcg
     4561 gctcacgatc cggtactgat ggcccaggcc atgcgtcacg cagtggtagc cggccggcag
     4621 gccttcctgg ctggacgcat gccgaaaaag acgtataccg ccagcccgtc gtcacccacc
     4681 tccgggctca tcggcagtta accggcactg tgcactccgc cgtgaatctc cggagtcagc
     4741 ctgtgaccgg cacagcacgt cgcatccacg cgcctcgggc cgaccggtac agccacagcg
     4801 cgtggccgat gcgcagccgt ggcggtccga atgcgccggc aaaccgctgg gcacgataca
     4861 accgcgccgg ccaccttccg gcgcactgat tcatgggctg ttaatggaaa ctcaggcaca
     4921 accgcgtccg atacgcagct tcgtcattcg tcaaggacgc atttccccgg cacagcgcca
     4981 ggcgattgac cgtctctatc cagagtacgg catcccgttt gcacccgcgg cgctcgactt
     5041 tgctcgggta ttcggccgca gcgcaccgac gattctggaa atcggtttcg gcatggggca
     5101 ggccaccgca cgaatcgcca gcgaacaccc gcagttcaac tatctcggcg tcgaggtgca
     5161 tccgcccggc gtcggcacgc tgttacggct gatcgagcaa caacagttgc acaatctgcg
     5221 catcatccag cacgatgccg tggccgtgct gcacgcgatg atccctgacg ccagcctgca
     5281 gggtgtacat attttcttcc ctgacccctg gcacaaggcc cgacaccaca agcggcggct
     5341 gatccagcca gaattcgttg aactcgtcgc acgcaaactg gcaccggaag gttacctgca
     5401 ttgcgccacg gactgggaac actacgccga gcatatactg ggcgtgctcc agggtgccag
     5461 cagcctgatc aacaccgcgc gcgattatgc gccacgccct gactaccggc cgctgacaaa
     5521 attcgaacag cgcggccttt cgctcgggca tggcattcgt gacctggttt tccgcaaacg
     5581 cgtggacccc gccacgctga cagaggtcag ccgaccttcc tgatccagaa ccgcaactgg
     5641 cgcgcttcct gctcatgctg cagcagatca tgccgggtct gtcggcaata tgagggaatc
     5701 tcggtacgca acgactcgat gtcgccctcg accagcaaca cctcaccctg atgcatggca
     5761 cgaaccccgg atgcaagctt ccggatcagc gccccacaac acagaccacg caagtccacc
     5821 tgtcgatcat gttccatcgg caccttcctc gatggccgcg gatccggcgc cagccggggc
     5881 ggccgcagcg actcagaatt cgttctgcag ctttttgtag ccgcgaacca gttccatgtt
     5941 ggtttgagcg atgtcctcgg aaaaatcctt ttcactgaaa ccaacctgct ggatcaggct
     6001 gagatcggaa tcggcatgaa tgttggtggt ggatggaata tggaatccat ccggcacatc
     6061 acagccttca accacggaat tgtgcctgac cacgcactgg ttgccgaccg tgcagttgaa
     6121 cagcacgctg ttgaaaccga tgaaaacccg gttacccacc ttgcacggcc catgcacgat
     6181 ggaacggtgc gcaatcgaac tgttctcacc gatctcgaca cgcgccccag acttggagtg
     6241 aatgaccaca ccgtcctgaa tattggaatt ggcgccgatg atgatcggtt ccattgatcc
     6301 ggaagcattg atttcgtcgg cacgaatgac tgcatacgga ccgatgaaga cattggggcc
     6361 aacaacgacc ttgccacaca gaatggcggt ggggtcaaca aatgcagact catcgatgac
     6421 gggcagatcg ccgttggggt ttttacggat catgaagtgc caccagttaa attgggttgc
     6481 cgaactatac ccaatttcat tccgatccgg aagccggccc gctgtgccac cataaccgga
     6541 accgtgctgc cggccgtggc ggaataccga cagccatcct ggccacgcca atgacgagtg
     6601 gccccgacgg atgcactgcg gcaccgtgtt tcctgccaga acgcccgcca tcgacagcac
     6661 gtcacctcgt gcatttcagc gcgccgggcc atcgtcccgg ccatcctgaa acagccccgc
     6721 aatttctcag aacgcatact ggagcatgat gcggttatca agatagctgc tgcccagcaa
     6781 cgggatgttc tgttccacgc ccaaccgctc acgcagggtc agaccgcgca gtttgccggg
     6841 cacatgccag gcaacgtcca gcatccaggc gcgggcgttg gccacatgcg gctcggtgtc
     6901 gtattccgac acggtcggca cgacgtcgac ctgcccgcca tccagatgca ggcaggcacc
     6961 gagcatccac gcatcccccg gcgcacgcgc cgacaccaga ccatagttca tcaccgtggt
     7021 gtagagcgga tcggtattat aaatctgtgt atagggcgaa tacaggttgc cgttatacgc
     7081 ggtcgtgggt gcgcctgctg aaatcgccgt cacggtgctg ctggccgccc ggttccaggc
     7141 gacaaagaca ttgccgaggt cacggtcaag gttgatcccc agcttgaagc caagcacctg
     7201 ggcatccaca ttgccgatgg cgccactgcc gctttcatcg acgtattgcg cgttgatgaa
     7261 caacggatgc acacccggaa aatcatggta gccgccttcg agatacacca tatgggccag
     7321 atcgtaaaaa tcgtacaacc aggcgtgcgc cgtcacatgc gcatcctgcc agttcacccc
     7381 tgcatcgagc gttccacccg attcaccggt ataaatcagc ttgtcatatg gcagcgccgc
     7441 gatcgtgctc tgggaaaaat ccgtcgtggt acggtttttg tagtccgtga cgcgatcaac
     7501 attgaaactc agcccgggca tgggcgtgac cagcaggctg ccgccctgat acaggttggg
     7561 aatcataaat ccatcgctcg aattcaccca cgggttatcg atggcgatgc gcccgccgcg
     7621 cacggtgaac gccgaattgt gatactgcaa ccaggcttcg ccaagcagat cgacagtgcg
     7681 gttgaccccc atgagcaaac tgttcagata aggatagcca taggccgcgt tatagagttt
     7741 gctgtcgttg taggtgtaat tgtttgcgcc aatatcccag gcatagtacc aggctgcgcc
     7801 agcagaaaac ccgttccagt ccccggtttc gcccttgagc tttccaccca gggacagggc
     7861 attcatgtcc ttgaaattga acgtgccggc gccggtatat ggtgatccgg cggactgatc
     7921 agatgccgtt ccggcgtaat cgcgcgagaa atcatacaga cgaatgtcac cggaaatgtg
     7981 cccgccctgc agccagtcca gtatgccttc atccgcctgc gcctcctgcg ccatattcga
     8041 cattgctccc agaactgccg tcaacaacaa tacttttttc atcgcaccgc tcccatggtc
     8101 tgaaccccgc atcgaattgc aaatttgtgc ataacgacac acagcacgcc acgaatccga
     8161 caaacacgac acaattccgt cttgatacgt ggtgcataac gcaccgcagc aagagccgcg
     8221 ccagcctgtt tgcaggccac aacagaagca cgctaaaatc cgccgcacat tcacattgcg
     8281 cccgcaccca tgacatcacc cacccacgat tgcactccgg aaatcctgct gatcgaagac
     8341 gatccgatga tcggcaacag cctgctgcgc gcactcaacg acgaaggtat cggggtggaa
     8401 tggcagcgcg atggccaggg tggcgaacag gcgctgcgca cgtgtcagta cggaatgatc
     8461 ctgctcgacc tcggcctgcc cagacgttcc ggcatggaac tgctgcaaca gttgcgtggc
     8521 agcggcaata ccacaccgct gctcatcatc acggcgcgcg acgaagtgga cgaccgggtg
     8581 cgcggactcg aagccggtgc ggacgattac gtggtcaagc cattcggcat gatggaattg
     8641 atggcgcgta tccgtgcagt gctgcgacgc accgcgcgta acgatgtgca agtgatcggc
     8701 aacggggaga tctcgctcaa cctgcaaaca cgggaagtca gttatcgcgg caagacgctg
     8761 ctgctgccgg cgcgcgaatt tgccctgctg cacatcctga tcgacaatcc gggcaccatc
     8821 cactcgcgag agcagctcga aacccgtctg tatgaatgga accgcgaagt cacgcgcaat
     8881 gcggttgacg tgctgatcta ttatctgcgc aagaaattcg acaacgacat cattcgcaac
     8941 attcgcggcg taggctggat ggtgctgaaa cacccgaccc caccccattg attcgccctg
     9001 tggagcatct gcatgattcg ttgcctgttt ccccgacccg tctccgccgc acgccgccac
     9061 agcgccacac gccgcccatg agatcactca agcgccagct catcctgtgg ctggtcagcc
     9121 tgctcacgct gatttcgatt gccgcggcag ggttctccta ccatcttgag ctcgaagaaa
     9181 ccaacgcgct gatggataac cagttgatgc acatcgcccg cagcgtggac gagggctcgc
     9241 aactgccggc aatgcgggaa cgtttcatgc acgaaaacga acagcagcgc gccgaggatt
     9301 ttgtcatcca ggtgtggatg aatgggttgc cagtacgcac ctcgcgccca gattttccgc
     9361 taccgcgcgc actgcggaac ggattttcca tccagcgtct gccgggcaat ggcggcggtg
     9421 atgcctggcg ggcctacacc atgtactacc ccgaccgggt ggtccagatt tcccaggacg
     9481 aaaacgtgcg cctggacatc gctcgccagg ccgcgttgcg tgcccttgtg cccgacctgc
     9541 tgctgatccc gctctcgtgg ttgctgatcg gactgatcgt cagccgactg ctcgaaccgc
     9601 tggaagccat caccgaagcc gcggcgcaac gccgcctcga cagcctggag ccgctgccgc
     9661 gcgacaacat ccccatcgag gtggaaccgc tgatcgcctc catcaacacc ctggtctcgc
     9721 gcttgtcgca ggcactgaac gcgcagcgcc gtttcctctc cgacgccgcg catgaattgc
     9781 gcactccgct gaccgcgctg caactgcaga tcgacaatct cgcccacagc cccaaccagc
     9841 aagagctcga cctgcgtatc gacgaaatgc ggcgtggcgt ccatcgtgca acacacctgg
     9901 tggcgcagct gctgcgcatt tcccgctatg aggccgcgca ggccacgaca cgacgagaac
     9961 cggtcgatct caacgcatgc atgcgctcac tcctcgccga catcgtgccg ttggccgacc
    10021 accgccacat cgaccttggc ctgactcgcg acgacgcggt cagcctgtac agcatagccg
    10081 aagatctccg cgtcctgctg agcaacctgc tcgacaatgc cattcgctac acgccgcaat
    10141 acggccgcgt cgacatccgc atcgaacgca cgccggacac ggccatcgtt gaaattgccg
    10201 ataccggtcc cggcattccg ccgcatctgc atacgcgcgt gttcgaccgg ttctttcgtg
    10261 ctgccggaca ggacaccgaa ggcagcggca tcgggctggc gatcgtcaaa gccattgtcg
    10321 aacgtgaaca gggttacatc acccttgcca accgcattga aggcagcggc ctgattgccc
    10381 gtgtcgtgct gccgctcagc accgaatcgc gcccctcgga ggaacatgcg gccagcttta
    10441 agcaatctct cagggacaag acttcccact gaaatatttc tgtcattaaa atctaaattt
    10501 gatggcctag aatccccgaa atgcagattt ctccgtcaat tgcggataac gaatacattt
    10561 caggggagct tcaaccatga ccatcgacca tctgctaaca ctggccaacg actccggcgg
    10621 aaccttgtac gtcatggttc tgttgttgct cgtggcgctg accgtgatca tcgaacgtgc
    10681 gcgttacctg tcgagcatgc agcgcggtgg cgcccatctg atcgcactgc tggccgaaca
    10741 gcagggcaat ctgtccgacc tctcgctcac agaacgccag tcacgcctgc cgcacgccag
    10801 cctgtttgaa atcatccagc gcgattcgag ccgcaccacg cgtgaaatca tgggcgaaca
    10861 cctcgaagaa gccatcatgc acgaagtgcc gcaactcgat cgctcgctgt gggtactcga
    10921 caccgtgatc acgctcgccc cactgctcgg cctgttcggc acgatcatcg gcatgttcaa
    10981 cgccttccat gtgctgggca gcatgcacga caaccctatt gccatcacgg gcggcatcgc
    11041 cgaggcgctc atcgctacgg cctcgggtct gttcatcgcc atgctcggcc tgatcgccta
    11101 caactacttt caccagcgcg tgcgcctggt gctgcaccag cttgagacca tcaagctgat
    11161 gctgctcaac cgctgcaacc agatcggtgt tgccctcggc cacggcgcaa gcccggtcga
    11221 actggtgcgc accccgtcgg cccgaggctg atatgcgcta catggaaacc gcgaaagcgc
    11281 gcatcgagat catcccgatg atcgacatca tgctgttcct gctggtgttt tttgtcatgg
    11341 tcacgctgcg catgatccca tcgtccggtc acctgaccaa gctgccgacc agttcgacgg
    11401 cggtcaccat accgccgccc aagctgctgg tggaaatcca gcccgacggc aacctgctgg
    11461 ttcagggccg gatcatgacc ccagcacaac tcacaaccat gctgcgccag cgtgacaaca
    11521 gcaagactgc catcaccatc gctggcaacc agacagtcaa cctgcagcag atcatgacca
    11581 ccatcgacgc ggtcaaaagc ggcggggcca ctgaagtcgg tctgtcagcg cgcgaaaatg
    11641 gcagcgggca gtaaaccggt atgacttcca tcgccctcac cctaccggac gcgccccagc
    11701 ctcgcagcgc gcgtggcgac aacaagacag cgcaagcttt cggcatggcc gtggcgatcg
    11761 aactcgccat cctgctgatc gctggcgtcc tgctcacaca taccaaaccg gcgccgaaat
    11821 actccgaacc agtcaaactg actatcgtca gtgaaccgac accccccaag ccggtggtgc
    11881 cacccaaacc cacgcctcct gttcccaagc ccaaaccggt agtccgccac atcgtacagc
    11941 cacgtccagt ggtgaccccg ccgcccccaa aagtgcagcc agtaccaacg ccactgccca
    12001 aggacgaagc gcctacgcca ttcaccgcac cgccgccacc tccaccggct ccgccgcagg
    12061 ttgcaccgag caaccccagc cagctcgcgg actaccgcgg acaggtgcac gccgccgtgc
    12121 aagcggccgt gttttatccg ccggctgccg ccctgatgca tttcaccggt cgggtgcgtg
    12181 tacaatttca cctgctggac accatggcaa gcgatgtgtc ggtgctgaca tctagcggcc
    12241 tcggcctgat cgatcgggcc gcagtcgatg cagtcaaatc ggcgcaatat ccacaaccgc
    12301 ccgcaccgct gcgtggcaag gaccaggtct accaggtgtg ggtcgaactc acgcttaacc
    12361 aagggaactg acagacacac gtgcataacc gctcattgca cttcggcctg catgcgctcg
    12421 cagccctgtg gctgctgtgc gcagcaacca ccgcacgggc taccccgact gatgactcgg
    12481 tctgggtcgt gctcggtccc cagggacagg ccgttgcgcg ctacctgaca tcgcagcctt
    12541 gtcccgacat cgtcgtcgac ggcagcaccc accgcatgca cttgcgcgct gctgctgcgc
    12601 ttgaggcgca acgcccgacg gcatcgccag ccagtgcatc caaggctgcc aacttcgcac
    12661 agcgcacttg cgaattcgac ctgccggcag gaacgcattc ggccagcatc cacggcagca
    12721 acctgccggt tgcccatgac agctaccgcc gcatcgtcgt catcggcgac accggctgcc
    12781 gcatcaagag cggcgaaccg gcgcaggcat gcaacgatcc gcaacactac ccgtttgctg
    12841 ccattgctcg cgccgccgca cgctggcacc ccgatctggt ggtccatgtc ggcgactacc
    12901 tctaccggga aaacccctgc cccgacaacc agcctggctg ccagggaagc ccgtggggtt
    12961 atggcagcga cagttggcgt gccgactttt tcactccggc acgggcactg ctgcaggccg
    13021 caccgtgggt catggtgcgc ggaaatcatg aatcctgcca gcgtggcggc cagggctggt
    13081 ggcgttttct cgacccgaga ccattgcagg ccgggcgcga ctgcaactta gccagcaacg
    13141 atatgcgcgg caatttcagc gacagctacg ccgtaccgct tggcgacagc gcacagatcg
    13201 tcgtcatgga cagcgcggcg gtcccgcgcc agccactcga agccgacgac ccgcgtactc
    13261 aagccctgcg cgccagcctc gaacaggcgc tgcaactgac ccaccaggcc caattcaatt
    13321 ttctggcgaa ccaccatcca gtgctgggtt tcgccgcttt ctacggccac gatggccagg
    13381 ccgaactggc accgggcaac agcgcgttgc aggccctgct gcgcaaaatg caaaccggtc
    13441 tgtatccggc ctcggttgac gtcctgctct ccggccacgt gcacgtctgg gaacaactcg
    13501 attttgccgg aacttacccc agccagttca tcgccggatt ttccggcacg caggaagata
    13561 tcgtgccgct gccggcgcat cttccccccg atgcccaacc ggccccgggc gcgcaggtcg
    13621 cgcatttcag ctcatgggtc aacggatttg gtttcatgac catgcaacgc cgtgacaatc
    13681 agcgctggga tgtacagatc cacgaccgtg agggcaaggt ggtcaacacc tgcaccattg
    13741 aaggccgaca ctcgcagtgc aagcgtgacc agatagaaga ctgaccgacc actaccgctg
    13801 cacagtcgtt acggcagcag accagcgatg cgcgatcgcg cacgcacgag ctctcaacgg
    13861 ctcagcagga tcaccgccca ggtgatgatc acgttcagca acgaaagcag caccgcggcg
    13921 ctgcccatgt ccttggcacg tttgatcaag tgatgatgtt cgaacgatat gcggtcgacc
    13981 gctgcttcca ccgccgaatt gagcagttcg accaccagca ccagcagcac cgaaccgatc
    14041 atcagcaccc gtgccaccgc accatgctcc aggtagaacg ccaatggcac catggcaagc
    14101 gcaagcaggg tctcctgccg gaatgcatct tcgtagcgca aggccgcctt gagtccgtcc
    14161 agcgaataca tcaaggcatt aaacacccgt ttcagcccgg ttttgccttt atatggcgat
    14221 tcctgcacgc caatttctcc tgtttcgttc atttcgacac tccgcctgta atccttgcgg
    14281 caatacgcca tcaaaaatgg ggagcatagc ccaatctgga cggatatgaa cagaaaaagt
    14341 cgtgaagttt ttgtgacaaa tagcagaaaa tccgatcaca caatgcgaaa ttaaccggaa
    14401 ataccacgcg gtcgcaaccc ggtgacccat aggacaccga gtactatccc atgtcagaat
    14461 gggcagggaa tcacgccgca gctcgtaacg cgcgtctgct ccgaacagtg gctgccgttg
    14521 cgggttggcc ggcataaaac cctcaggctg acaacacacc atggcaggtt tctccgccgt
    14581 cccatgcctg atgcgaccga cagcttcagc ggatgatggc cggctgaggc tgacgcacat
    14641 cacggcgcca cgtcagccgt ctgcccggaa caagcgtggt tgtgacatac cgatactaga
    14701 ctttggccaa ccgggaaatc gtatcgggat actgttcaac catccgcatc agcaagatgg
    14761 cctgcgcgtt cggtcgtgcg cggccctgct cccaattttc cagcgtgcgc acattggtgc
    14821 gcaggcagcg cgcaaacaag gctcgcgaca gatggagccg tctgcgcaat tgcagcacat
    14881 catctgcccc catgtccggc actggattat gcgccaaggc atgatgccgc aaggtaatct
    14941 ttcccgaccc gttggcacca tgccaggact gggattcaat cagtttgtca aaaatcacat
    15001 gatccatggt ttctccagaa tgaacgacaa taaaccctgt ttcaggcgcg acctctatgg
    15061 ggtcccccca atgacctggc ctgcaggcgc agcacactgc tccgcagcag cgccactcaa
    15121 gcccaatccg ccttgctcga cgggtgcggt gcaactgcgg taacgttgcc gcatggcctt
    15181 ggtgctgttg tattcggcat aactgtccgg ccgccacatg ctgccaggat ccagccccat
    15241 gctgcgacat tgcccgacag tgaacccggc tccgcaattc cccagataca gcatattggc
    15301 gcccgtcaaa ctgttgtcgc tccccatcgg gatatccaca tggcgctgtt caatacggtt
    15361 gaagaatacc ggctcgaacg gcccgttggc tgccgaggct tccgagcgca gccagccatt
    15421 accggtggta ccgaacaggc cattctgcgc ctgctgaaga ttgccggacc cattggcaat
    15481 ggcattgagc cccagatagg cttctgcact ggcttcaccc accgacagcg ctccttccgt
    15541 ggcaagaagc tctggtgtca gcaaggtcgc aaatcccatg gtcacaatga caaacagcat
    15601 cgtgaacgcc agaatgctcc atcccgactg ggtattgacg taacggtaga tcaggtcctg
    15661 ggtttgcggc atattgccac cgctccactg gctgaacagc accccggcag tggccacgtg
    15721 agccggatcg tcacatgagg aggtactgac cccagcgcct ccggtcacgc agatctgtcc
    15781 ggtctggcca gttgggctcg cctccatggg cgtcgcgaca aaccacaccg gcttgacata
    15841 tccactgatg gtggtggtga ccgaactggt aaaccatccc gaactggatg aagtctgctg
    15901 gatcacattc ggctgatcca cagccacaaa gccgaatgcc gcgccgtagc gccgcatggc
    15961 atgccccacg gccaccatca ttgcctgcca gtccatgcca tggaataccg ggtcgccccg
    16021 tgttccggaa aaggccgcga acgggttata accgagggat cccgctgcaa tttcatctgc
    16081 attacggtat ttcccctgcg ccttccacaa ctccccgctc cacggcgtaa aatccgcctg
    16141 ccatacacgc acctgaccac ttggcgtttt ttccaccttt tgtacggtga tgcgtccact
    16201 cgcattttcc ggcgaatact gggcaaatac atatgcggta ttcttgggaa atttctgcat
    16261 ggcgctggaa atctgctggt tggtaagccc gagcgtgccc acagtttcct tgcccagcgt
    16321 gatccacttg tcgccagaaa tcatggcttc ggacacatca agcccacccg tggccccggg
    16381 gctgaaactg ccctgcccga ccagacgaaa gtcatcctgt ggcacagcaa ctgacgtgcc
    16441 tggccaggta atccatgccg ccgcctggca ggtcgtggtg aacggcacca tgaccagtat
    16501 tgccaacagc agatgaacca gtccccatac cgaaccccga ccagcattgc ttaaacggtc
    16561 gcctatcccg aatacgtatc ctcgtttcat cacgccctcc attctgtctg ctccgcttgc
    16621 gcacacaatc cactcacggt tgctccacga tcggcgcaac atacggatag gccgcagccg
    16681 gcaaaccatt gaccgtatca tcgaggtagt cataaatctg gctggtacgg aacggatcga
    16741 taatcatgtc gctgtaggcc gtgggctcgg cacccatcgg cagactgacg gatttgctat
    16801 acggctgttg cggtatctgt ggcgtagcgg tctgctcgcc gaccagaaaa taattgccat
    16861 cgggcgtcac ccaatagcgg tcgaccaccg atagcacctg ataaccgata ttgccgctat
    16921 tggtgaacgt cccaccggat gcggtcccgg cggtcaccga tgcgacgccg gagtagttga
    16981 caatatatgg cgcgccataa ttgccgaaat acccccaggt gacctgacca ttgttctggg
    17041 tgtagatctg gtactgagta ccgttcggta caaagaaata acaaatcggg atgactgttt
    17101 gccagggttg cacgccatga cgcaacggct tgagcgaata gtaaagctct gcaccagcaa
    17161 tcgtcgggca gttgccttgc gcatctccct gctcggtctg cagcacaccg cctggcaatg
    17221 tttctccatt tgtaacgctg cctccggtat tgtcccaccc ggatggaatg acgggattgg
    17281 aaaacagaaa taccgcaccg cctgccggtg agatacattg cagaccaaaa gcccagggcc
    17341 atgaatttgg ctcgaacgcg ggctgacaat tcatcacgct ctgctgcatc cacacacgac
    17401 tggtcacgtc caccgccacc tgcgccgtct gcaccgtctg gccctgggca ttggtgctgc
    17461 tggtatacac tggcctcaaa ctgcgcacat agtcaaggta tccgccactg gcgccagcag
    17521 cattttccag gctgatgaaa tcggtgaaac cattgatcgg cacaaccgga cagcccgcat
    17581 caccggcatg attgatcagg catttgaggc cataatcggg gtcgcagagc acctgtccat
    17641 tgctgctctg gcagccgggg gtcgtggcat tggctccggg gttgaccgtc ggggcatcaa
    17701 acgcgccacc cagtgagaag acctgtgcgg cagtgaccgg ctgcaattgc tgattgacct
    17761 gctgccactg cagactgccg ggcggcggca gggccccacc gacctgattg ccggtcagcg
    17821 ccgcggcaga ccaggcagaa aaaccggctg gcagggtggc atccaccgct tgcggcaggt
    17881 agacgccata gacgaaattc ggtgcgctgg ggatcagttg tacattgcca taacgcggac
    17941 gaccgctggc atcgacgatt tcctgccaga ccagatacat tggctggctg ttgccctgca
    18001 ccatgacctg ctgctggaaa ttgaacatcg ctgacgccga ccccgcccgc gtcatgtaac
    18061 cggacaactg tgtggccaac gccaaggcaa gcggaagaac tgtctggttg gccctcgcca
    18121 gcagcgcatc ctgcgcgagc ggattaccgt gactgtctgc agccgcgatg accggtatct
    18181 gtgcaacgcc gcttgcagcg gcatcgactt ccctggcatt gtccagttcg taagtcatgc
    18241 cgcgcgcagc ggtataaccc agaccagcgg tatagactga aaatcccaaa ggatagctcg
    18301 gcgcctgacc gactgattcg taaaccagca cggtcttgct cgacttgctg ccagcacagg
    18361 ccacgccgga cccggccagt aacgccgcca ggacaagctg tccgcaaatc aatccatcac
    18421 gcacgatcca cttcatgacc caactccgtg gcaacgcagt tcaacgactc gggatcacgg
    18481 ccggactcag gccggcaggt ggaggcaggc ctccactccc tccagccgga ttcagcgccc
    18541 ggccagcgag catcctgacg tcctgcctgt cgacatcatc caccgacagg ctttcgatgc
    18601 tgatgtcgcg cccgccgatc ctcgcgctgg cagtgccgat cgactcgacc accccgaggc
    18661 cagatgcgtg ttcgccaacc cgtacatagg acacttgccc cgcagcctcc accaccacct
    18721 gctttccatc gttacgctgc aggatcccga tcagatgtgg cccgactgac ccagacggac
    18781 gggtaacgac gttcgcagct ggaaatatcg gcgcggtcag cgcatcggtg cgtttttttg
    18841 cgctccgcga cacggggtcg ggccccggga aacgcaccgc agggtagccg ccgccaaaac
    18901 ccgaaccggc cctgccactg gcctggtcaa gctggcggcg caattcggca gcgaaaatcc
    18961 tgtcggcgtt cctgagcatg aattccgcac gcgccttttt ggtctgttcc tcaagcaaac
    19021 tattctgcgc tttggtcagc tcgagcacgg cgcgggtctg ctcgatggcc agtgtctgac
    19081 cagtgaacgg atcgatgtcg gctgcggttg aagtgagtgg ttgcacccac accgatccgg
    19141 cgcacatcac cgggatcacc agacgaatga atgacggtct catgaatggg cctctttcaa
    19201 ttattgaccc caacagcagc ttgcgcatac gccacattgc ctggcagggt ggtggaaaca
    19261 agcgcggtat acgggggggt ggtcatgttg acatcaacac cgttgtcagg cgatcgcacg
    19321 tcattgcctg cattttcgaa ccggatcaca ttgccatagc catcggtcac atcgtcacgc
    19381 gacagagaga gcgcgcttgc caggtcggtg gacgatccgg taaacatcgg ggcagccacc
    19441 ggcagccagc caatcaggtt ccccggattg gtggaatccc agcgggccgg atccgggcta
    19501 cctgtaccat cggaccagta atcaatggac gaagaccggg tcggatcggc ctgataacgc
    19561 gccaggtaat aggtttccag ggcctgcaca atggtgcgca tcttgccggc agtaacaatg
    19621 aaattgctgt tggcgaatgc gcgaccatcg aacagtacgc cgctgtcatc accagcgagc
    19681 agcaagctgc catttgcgtt caggccagtg cccgtgtcca gcacgttatt cggaccggtg
    19741 gatacgaaag ctaccgtgtg gtagtacagc gtcacgccgt tgctctgcat cgatgcctgc
    19801 ggattgatga aaatgcacag tggcgcgcca tagccatcat ccaccagact tgctgccgca
    19861 tgtcccgagt aatccgacca tgccacaaaa ttgccgctgg cggcgctgca gcgcgctccg
    19921 gcggccgccg tggccggcgc catgattccg gatacgccga aattgatttc cgcaccggga
    19981 tcagcctcga tgctgccgat attggccaga tatgcggcag caaaccctga ctgtaattcc
    20041 gacaggcggt tacgggtgtc ggccgcagac tgaaaaccga tgaggtattt cagttgcggc
    20101 gcaaccgcaa ccgtcatgat ggccatcagg gcgatggcca ccagaatttc caccagactg
    20161 aatccatcat cacctgcagg tagcatcatg ccggcagcct ttttcaatgg tagctcagct
    20221 tgttacgcag ttcgttgggg tcataggctg cggccagcgc ctcttcaaga ctgacctggc
    20281 tcgaacgcac catttctgcg agtttgttgt tcatcagcac gttgcctgaa tcgcctgact
    20341 gtccaatggc attcatcaat gtttgcagtt tgtcctcacg aatgatggat gcgatgttcg
    20401 ggctattgat catgagctcg taagccagca tgcgcccctt gccgtccagc cgacgcacaa
    20461 gcgtctgcga catgacgcaa ttgagcaccg aggagaagat attgcgcgcc caatcctttt
    20521 catcggcagg aaaaaatgac agcacccgat caatggtttt gatggccgag ttggtatgca
    20581 gcgtggacag caccagatgc ccggtttccg ccgctgaaaa cgctgagcgc atggtttcat
    20641 aatcacggat ttcgccaatc atgatgatgt ccggatcttc acgcatggcg ctgcgcaacg
    20701 cggcatcaaa cgaactggca tcccgttcca gcccgatctc gcgcgtggtc actttgcatc
    20761 tcctcgaatg caacggatat tcgaccggat cctcgatggt gatgatatga ccttcacgcc
    20821 tgacgttcag accttcgagc aaggctgcaa gcgtggtcga ttttcccgat ccggtggcac
    20881 cagtaatcag gatcagcccg gtcggccggt cgatctgctg gatcagcgct gcaggaagac
    20941 cgatgtcctc gaccgttggc atacggctat tgagtttgcg catgacgata gccagccggc
    21001 gtccgttggc atacaccaga tcgacgcgca accgccagga atcgaacgta accgcaaacc
    21061 catcaccatc cttaccgctg ttacgcggtg cggccagttt gtcggccggt gtattgatgc
    21121 cgaaatcccg cagcaacatg gacacatcgg aatcggaaat cagctgctcg ctgtcgcgac
    21181 gcatcacccc catggcacga aaccacaccg gttcgccaac cgtcaggtgt acgtcggaaa
    21241 cgaaggtatt ctgcagaatg tcgaacaagc gctgtttgag gtcaatcatg gccccccctg
    21301 gtcatcggct agaattcaga taccgcatgc acattcaggc tgctgcccca gggcagcgag
    21361 gcacttaaca tgatgctgta tggcggcgat gcggccaccg tgtaattgtt aacgctgatc
    21421 ccgccctgcg gatcccatgc cgtgaccaga tctgctgcgg acaacccgat ccgcgccgga
    21481 agcacggtct ggattatcgg cacggcgctg gcaggcgtga catcggttcc ataacatggc
    21541 agtgccaggc catcgcctgc acaggcgctg tcacgaaacc agttgatgtc atcactacga
    21601 tcggcgtcca tttcgttccg atcttcgaaa tacgcagtca acatatcggc aatgcgatag
    21661 agtgtcagcc gcgtctgctg cacgcgcagg gtttcgagtg cacgcccgga taccaggaca
    21721 taaggtgtga gtgctggctg gccattcagc attccgggac tgaacgtgcc ggtagcctcg
    21781 tccaggccag tgcccgttgc ccctgccagg ggaaaccaga aggcaaacac gtggtaaccg
    21841 atgccgttgc tgatcacccg gtcactcacg tcgaccgcgg caccggcggt ctgaacgcct
    21901 gcctggacga gcattccagc caggggcggt ctccccgact ggctatcgac aacccaggca
    21961 ttgttgccat accaggtcag cagttgcttc tgcatggtag ccagttcggc ctgccgtgca
    22021 ccggtgtcac ccaccaccat cgaacggtcg agcgcaccgg catgcttgag ctgagcaacc
    22081 atcgtaccca cagtcaacgc cagccccagc gcgagcagcg cagcaaacag cacggcgata
    22141 taaccggatt gcgctttcat gcgtcgttgc ccccggaaat cagtacttgt tgatcagcat
    22201 ggtgacgtcg gagccacttt gcgtgcaggg ccaggttgcg ttggcgctac cgctttgtgg
    22261 catcgcctgg ccctgactga aatcctgtcc gtcacatttg ctcatgagct cctgctggtc
    22321 ctgcagtggt aatccgctga tcatgagcga gtaatcctgg atgccacccg tattctgctg
    22381 aatccagata tatgcgttgg aaaaccccag cttggtcagg tcgagcggtc cctgcgtcga
    22441 tcctgctgca aggccggtcg ccgagaccgg catggcggaa atatagtctt cattgccata
    22501 cagggccgtt gtcgcctgat tgcagaaatt gttggccggc gtggctgcca gcgccttgtt
    22561 gaacagcaga tcgagccgtg ccgggacaca gcccgtcctg gcctcgtaca gggtgacagc
    22621 cttggcgata tcctgggcct tggcaaacag tgcggtgact ttggaatcat ccccgctgga
    22681 caacgcggtc atgagacccg ctgtgaggat gccaatgatg gcaatggtga tgatgacttc
    22741 aacgagactg aaaccgcgct gcatgttgcc cgggtgtcgc gacctgttca taaataacgc
    22801 tccttgcatt cgccaatatt caatagcggc tgatcagcat ccccacactg ttgtctgccg
    22861 ccacatatac acatgccgct ccgttcacaa aatcatgagg caggttgccg gttgccaccc
    22921 cggtgtaatc cacgccattg caggctgcga gcagcggata cagcgcatcg ccaggattga
    22981 tgacctcgat ggcataattg ttcggggtgg tgccagtcag attttgccga atcagcaagt
    23041 caccgttttc cacacccaat tggttgagca gcaccccttt gccaccatcg ccaggcatcg
    23101 gtgagagata atcctgattg ccataattcg cttgcgtgga aacggaacag aaattgttgg
    23161 ccgccgttgc cagcaacttg tcgaacagca ccgagacatt gttgggcacg catccggtat
    23221 tacgctggta cattgacacc gcattggcaa tttcctgcat cttgctcatc agtgcggccg
    23281 tcttgctttg ttcactgcca ctgaaggccg acatggcagc ggcagcaaga atgccgagca
    23341 tcgcaacagc aacgatcatc tccaccagac tgaatccacc ctggcggagc acccaggcac
    23401 aggtcatccc ccgccacaac cggatcatcc tggtcggccg acgcggcaga cgggatctgc
    23461 gatatcgcac accactcata gcttctgccc tatttgcacg tacatcggat agagcgtgac
    23521 ataaaagaaa ccgctcacaa tcaaaccgcc aaacaactgc gacaggtagg taatcttgat
    23581 tttcaggatg tcagtcatga cctcgacatc ctgttcgaga tcggtcacca tgccgagcag
    23641 tccctcggcc agacttgctc cgccttcctc tgcagacttg acgccattga cgacatatgc
    23701 cggaaatccg gcacgttcga cggcaaccga aatcggtacc ccgagccgga gtatcttgtc
    23761 catgcgatgg aacatatcct gcgaatccag tcgcgcgcag cccttgcgca caaccgccgc
    23821 agattcatac gccgaaatac cgccggcgtc gtacagcaga ctgaaggaag tccacgcgga
    23881 cgagtggtca gatttctccg agatcattcg ccagttatcc cagtaatccg ccatctctcg
    23941 cacaaaacgg ctttttatca tggcatcacc ggcgacgatc atgcctgcca gcagcacacc
    24001 tgcaatgacc ttgtgcccag tgacccacag ggcaagctcg ttgtacagga cgatcggagg
    24061 cggcagatgg ggcgcattac cacctgacca ttgcgcaaaa aaatggatca tggtcggtga
    24121 cagccagccc atggcggcat agatgccgac caggccaacc acggccagca ctttcatcgg
    24181 cagaaaagtg gaatggattg ccgaacgcaa gcgctctgca cgagctacgt catcagccag
    24241 acgcacaaat gcctgcccct gttttccggc agcatcggcc gcacgtatga cattgagatc
    24301 ccgttcggga aaacccgccg attgcatcgc atcagcaatc tttgcgccgt cgatcaggcg
    24361 ctgctgcatc aagcgtagcg cattgaccag catcggatcc gtggtgaagg atattgccgc
    24421 ctccatgcca ctgatcagtg gttttccgac ttcgagccgt ttgcccatgg ttcgatagaa
    24481 gcgcaccagt tcacgcacat cgaatttccc ccccagccca ttaatggtgc cggccacatc
    24541 gattcgcacc ctggagacac gcaacccggc ccgtttgagc ttggcatagg ccgcatcact
    24601 gtcttcagcg tagatcaggc cattgactgc cttggcagcc tgcgtatgcg caaaatagcg
    24661 ataaccgatc aacatatcag tgccgatccg cccggatcat caactcgtcc atcgatgtca
    24721 cgccagccgc caccagattc agcccgcggt accagatggt ttccgtgcgc tgcacgcctt
    24781 cctgctcgat gcgggatatc gaagcaccct cctcgataag ctgccgcacg gtataggccg
    24841 gcatgagttc gtagatcatc tgtcgaccgc ggtagccaat atcgccgcag tgtggacagc
    24901 cgacagccac atacggtgta aactggtgca catggtgttc gcgaaaggta cgcaatgttt
    24961 ccgagcgcaa atcaggcgcc ttgcagtgct cacataacct ggcaaccagc cgctgtgcga
    25021 tgaccagtcg cagcaccgaa gccagggcat cacggttcgc acccagctcg agcaggcgca
    25081 acaccgcttt cggtgccgaa ttggtgtgca gcgaggtcac gaccagatga ccggtattgg
    25141 cggcggcaag caaggtcttg accagttccg gatcgtcacg tacctcgccc cagagaatga
    25201 cgtcgggcgc ctcgcgcaac aatgccttga gatattcgcg tcccacgtcg ccttcggtct
    25261 gccgccggtt cccatccatc ttcggccgcg gcagctcatg ctgtatccac atgccattgt
    25321 gatattcaac cgggttttcc accgtaaaca cccggcgctg gatcggattg atttcacgca
    25381 gaatcgaata catggtggtg gtcttgccag agccggtcgg tccggtgacc agcaccaacc
    25441 ccgtcggcgc atgaatattc ttgagaatga tttcgcgcga cccggaatca aacggcaacg
    25501 agatgaaatc cgtttcggtt gactgcccgt cattgatccg gatcaccaca ttcttcttgc
    25561 ctgtcggact catgacggct tccatgcgga aatggaagcg accgaatatg tcatcgaatt
    25621 cggcacggat gtccggcgga gacccggaaa attctatttt tgcctcctgc ggcccagtct
    25681 tgatggcttc tgacaggcca cacgagctga tcagcagttc catggtcgaa tcgaacgctt
    25741 cattcctgag cacggtgaag atcgacccct tcccatcaac cttcaggcca atctggccca
    25801 ccttgcgcgt ggcccagatg aatatgtcgg atgcgccggc atagcaggca tgacgcaaca
    25861 atgtaccaat gacccgaaac acaagccccg gctcctcagt cagtgtgccg cgctgcaatg
    25921 ccgagtgata agcggccacg gccgccttga aggcctgttc ggtgttggcg aaatatttac
    25981 ggtaaatctt gaggatggtc tgacctgaag cgatgacgat ggcaggctga agatcaaaaa
    26041 actggttgcg tgcctcattg acgcgctcct gcgcatccac ggcaatcagc atgccaccac
    26101 gggcattgaa cccgaccgga acgtatccgc tgtagtgaaa acgcacgcgc gccaggatgt
    26161 cctgcaaacg ggccacatca gtcatgtcca gcgtgtcggc atcagccagg gcaaagtact
    26221 cgtacccggt atgcaatgct atcgctcgtg cgaccatctc ctgtgaaacc agtcccatgt
    26281 cgcgcacgat caccggcaat gactcatgcg tttcatcctt gcgcttaagg gcttcgcgca
    26341 cgtactcacg acgcgccccc agcgcaatca ggatattttc caacgcctcg cctgcaagat
    26401 ggccaacccc gtccccaata ccgggatgcg gcgcatccat gcctgaggat accgctggcc
    26461 cggacaccag tccggccagg ctgccggact cacgctgcct atcctcgaca cacgaatcag
    26521 ccacaatgca cgcgtgatct gtatcgacat gtaccgctga cagtgactcg ccattgtcag
    26581 cggatatcgc caggctgccg ggcaaaggcg atccctcgag gtggcctgtg gtcggtactg
    26641 acaggtttcc gaccgcaccg accttcttcc ctgtgagaaa aaaagccatc gtctcagcct
    26701 ccaagaaaac cgatgcgcat gactgcactg tcctgccgga tccgaatcgc ttcaagatac
    26761 ccacccgttt ccgggatatg cagaatgaag tccgtcaaga atccgagatc gtcaaaaccg
    26821 gtcttgaaca cataggaaac ctggcggata cgctcactgg ttcctggtag cggcctggct
    26881 attcgtccga gatccagtgc accttgcgcc accccggaca ccgtcaatgt ccccacgcgc
    26941 aaatgatgcg catgtgcaag attgaacaac gcgacagctc catacagtgc ggtaagccgg
    27001 atcggaccca gatgctgttt ggcaacggga atttgtgcct cgatcgcaac cttcatggaa
    27061 cgcgcctgct gcagatccgt agatgcggta gcgtagtctt ttctggccgc aaccaatgcg
    27121 tgccacaagt cgatatccac cgtggacagc ctgattcccg tcgcaaacat gacggttgcg
    27181 accaccattg acgatgcatg cctagggaat ctcattgccc tgctccatca tggccagcgg
    27241 tccggcaatg cgcacgcact cataacgcgc atacaattcg tttccggaac cggacaggga
    27301 atatccgctc atgtggcacg gcggcggcaa cgccaccggg cgcagcgccg catcaatctg
    27361 agcacgatcg gtgatgcgtt ttttatcctg caatggcagg ataagcgtgt gtgtttcgct
    27421 gacggcactg gcctcgctct ccacacgggc accctccacc cacaggcgcg tcgcatgatc
    27481 cagcagcgac gggtaatcca ccgccatggc ctgccccatt gccggtgggt tggactgcag
    27541 gcgatcaaga atcgcggact cggtgcggac gcgctgcgcc gcaatgtccc tggtctgccg
    27601 cgccagcaac tgcgtggcat gcatacagta cactccgacg ccagcggaca gcgtcagcac
    27661 cacagcagac aacaccgttg ctccctgtgc caatgccggc acggggattc cagccaaatc
    27721 cgtatcaagc ggataacctg gcgtctccag cagcgcaacg agaaattcac ccccagtgta
    27781 gatcggcatg gtgttgatat cgacgttgat gctgttgatc gccgcaaatg cggcgatgat
    27841 ttccgccaga ttgtctggcg ccaccgacgt ctgcacaaac acccgaccat tgcccggaaa
    27901 tgccatcagg atcagcagat ccgactcacc aaacaacaca cccgtcacac acggggggat
    27961 caggttgtcc agcgtcatca accgttcgag tgcccacgcg aagggcatga ccttctgcct
    28021 cgacaacact ttttcttcac tgcatgccca cacggcaatc cctgcaccgc cctgaatcac
    28081 actacgcagt ttgcgataca gacccagttc gcgctgaaga taactggagg cctgggatgc
    28141 actcatcctt ttaggcaaat ggaaacgata atcctccgcc gtgtcatcat ccgcccgacc
    28201 tggtggggca tataccgctg acacgagcac ggtatcttcc tggggctcgg aaatatgaac
    28261 cacgccacct tccggcaaaa tttcataata caaatgaact ccggattcga tccggcaatc
    28321 cagcgtatat cgcgttgcaa ccgacgatct ggatgcagcg aattccgctg agggatccgt
    28381 tctgcgccgg aacagggtag agatgttcat cacaggctct ctgccacgag cgtatccagg
    28441 tgtcggggag gaatcaccgt gctttgcagc aggattacca cttctccggc agactggtcc
    28501 ccattgccgc ccagcggcac tttgacaaac ggcaacgtcg tggtcgtgtt gttgtcggta
    28561 ccggtacgaa tcccacccag aatcaccgtt tcaccgttat gcacgattgt ctgcatcagt
    28621 acgcgttttt cggtttcctg cggcgcctcg agcgtactcc cgcttactgt gaaattctgc
    28681 atcgactcaa tggcgctgac cgtgggtgcc agggtaatct gcgcctcgtc atcgctgaga
    28741 atgtcaggca tgaccgaaag actgacgcca ctttctgcaa aactggttgc ggcaccagtc
    28801 gacgtcgcac tcagggttgc cgtcgtcatg atgctaccca gatagggcac gctcgttccg
    28861 tcaaaaattg ccaccggaga acgattcatg gcggtcacgg aaggctgggt caacacggag
    28921 acgcgtgcat aatttctcaa cgccgcgatc acgctgctaa tggttgcggt ggtgaaattc
    28981 aacgtcatcg atggattggc aatggatgac gccccgccgc tcagggacac tgccgcgtgc
    29041 gagttctgca gcggggcaag tacccgtgac cagtcaatgc catagttcat gctgtcatca
    29101 agcgacacct cgatgaccga caccttgata tccacgcggc gatcattgtc ataaacgaac
    29161 tgattcatga aacggtgcat gcgattgaga gcggcaccat tgccgcgcac ggttacatag
    29221 ccggtatcac gtgacacggc aacataagcg ttttgtcctg caatcgattg caggaactcg
    29281 tcaaaaccac catgcaacat cattgcactg gtgttcgaaa gtttggatga agcgttgaaa
    29341 gtcgcttgca cgcttcccac cgagccacta ctgcccgtcg aaacagcgcc tgttgctgca
    29401 ccgccgacgg ctccagcacc agccataccc gctgacgcgc cgagcgagga tcctgccgaa
    29461 ccactgccat cgatgccgta atctgaactc aatcgctgca tgagccgtac cggtatccgg
    29521 aatgtccatg cggcctgatc cgcaatcagg atgctgtgcg cttcacgatc ggtgatggcg
    29581 acatacccgg cattagccgc cacttgccga attgccgctt cctcgctcat gttgcgcacc
    29641 gccaccgaaa ccacgcggtc agcgtcgacc ccatccacaa acgacacggc atatttatcg
    29701 tttgcaatcg cacgcaaaat gatctgcatc cggccactgg cggtgatgga cacatctcct
    29761 cgctcgctgt ctggcggaac ccgttcgaca aatccacccg gcagatcagc cacgctatct
    29821 acacgtttca acccatacac cggttcctgc ccggcagtgg catcacggat tgccgcccga
    29881 gcgttttcat gcacctgagc atattgcggg ccggtggaac atccggtcag cccaatcacc
    29941 ataaccatga gccacccaca cctgccacac agttcagcgc acgaaaacat tgagcacctc
    30001 cccttcgtcc ttgctgagat aaccggttat ttttgctgca agcagaaaac ggttcaccac
    30061 ctcctcaaaa ttgctgccca caaaccgtgc atcctgtttt gcaagcaggt catcaccgct
    30121 ccagctcact tgataatgat tggcatgggc aaatgcgatc agtcttgatt tgaggctgtc
    30181 gccttcatga atgtcaaaat ccagtacctg ggctggcgtc acgccccgat tcgcgctgtt
    30241 cgcctccacc gcaacgaccg gcgagggttc tgacagcgat agcggttgcg ccacggttgc
    30301 ggcagaggca acgggcctgt gcggttgctg agacacacgc atcaccccgt tgcgacagac
    30361 atattgcagt cccgtccctt ctgccaggcg gcgaacggcc tcacctacgg tgcctgacac
    30421 gtcttgtgcg gaaatcgtcc ccgcatcgaa ttcgcccgat tcgaccagtg atatccccgt
    30481 tccagccagg attttgcgca gcgcggtcac gggcggttca ttttcaaagg aaaccggccc
    30541 gaaatgcaca tccagattgc agttcccggc gaccttgtcc gactcggcca gaccaggatt
    30601 gctggccagc agcataccca cgaacaggct aacgacgatc ccacatctgg tcagacacca
    30661 cggattcact gcgcacctcc gtcggtgtcc gctgcatcga ctttatcaat cttcgtacga
    30721 tcgtccgacg cgtacatgac tgacctgaca tccggttcgt ccacgtcacg gcaaaccggg
    30781 ctggcttcgt accgggttat gggatcaact cggactcgaa attcggcccg gcacccatga
    30841 ctgccggggc caacgccggg aggctccatc cccgccgggt tgctgggggc ccgataccgt
    30901 ttctgccggt ccacgacatg actttcccgc caaacagtca gcgggcgggc ggccacggct
    30961 gctgcggatg aacccggcgg aacagcaagg cgttcttccc aggtcaatcc atgtttgatg
    31021 atcgtgtgta cgggatctac ccgattcgca attgccgcat catgtgcagc atcctccggt
    31081 gccctcatgt ggaataccac acccagtttt tccaccccga ctggtccgac cagcctgatg
    31141 cgttgcccag tcctgatccg gttgacatcg gcaatgccgt tccagcgagc aagtttgtca
    31201 acggaaatac cgtaatgcac ggcaatacat gacagtgatt ctcccggctg cacgacatgc
    31261 acgtcagcca cctcggccac ccgatgaacc ggcagccgca tcaggcgcac atttcggtca
    31321 gaccgcaacc gcaccttcag tgattgcggc gcggaagacg catacactgc cgactgcacg
    31381 actgggttcg cgctcgcact cggatttggc tgaggctggt ggaaaatcag ttcgccactg
    31441 aaactgccac ccgcgaattc cggaaccacg cttgcgcctg cttcagccgt caccactcgt
    31501 gcgggcgtgg tttcgctctg aacgcgggca gcgcctgtta cgcgacctgc gcctgtcagc
    31561 gctcgtcctc ctgtataggt gatggtggcc tgcatgcctt ccaaacgcac ctgaatgacc
    31621 ggtgccaaac ggtcagctac gagatatgga aactcctggc gcacaggcaa ccgcgcataa
    31681 cccgtaggcg tgcggacaaa aaatgcggga atggcagcct ggctacgcag ttcgacatac
    31741 aactgctttc catcatcgaa cacttgcacg ggttcgattg ctgcatcccc gcttatccgg
    31801 tattcaaagt catatcctgg caactccgtt gccagtgccg gcacggccaa ttgcgccagc
    31861 aacagcatca gcacccgctt tttcaatgtg tatcctttct gtcagatgca ggaaactcga
    31921 tgtcctgcag aacaatcacc gccagtttgc tgcctgctgt cacattgagt tctggcttga
    31981 ggttctggta attgctcaag atgccgttcg ccgtatttgc ggtgatctgg cctgcggccg
    32041 tgccgatcga tccgttactg gtgccggtcg tattcagcac gatactgctg gagttgccgg
    32101 gttcgagcgc ccagccgatg gtggcgagca ggatgcctgt accgaactgc tcgaacagat
    32161 gattgtccag atctccaggc acccccgacc gcccttgcat atcgcccccc gtccatgcgg
    32221 ccatgcgaat cgacgctccc gtaggcagga tcagtctgtt gaacgccagc atcatgcgac
    32281 gctggccaaa cttcgtgttc tggtcgtagt tgccttccat gcgcgtgcct gcaggtatca
    32341 gcaatgtctt gcctcggatg gagtcataga cattctgggt cacgcgcgcg gatacatggc
    32401 ctggcaaagt attgttgagc gccgtcagta gcaccagtgg aatgacggcc ccttcctgca
    32461 acatcgtgaa gtcaggcacc gaaccgggtt ggatgggcgt ggctggggca gatccagccg
    32521 ctttccgctg cttttgcagc caggtttcat ccgcatcctg ctgcgattgt gcgccctgtt
    32581 tcatcagcag attgcgaatc atctgactac ccgctccggg cggtccgttc gccagactgc
    32641 ccagcccctg accggcggca ataccagaca accccatccc gccagacacg tctgcaccag
    32701 aaaccgcatc cactgcaccc agcggtgctt ctgccgattc tgcagctttc ccgaccgatc
    32761 ccatgtggta gtcctgcggt gtttgtgtct ggccatccgc agatgcagca gcaacggctg
    32821 agcggctgga gtcagcggcc ggaatggccg cagcggcgtt gtcagtgcca gccgatgctt
    32881 gctgttccgg ttgcggctgc ccgtgaattt cgctgcccac cccactctca tcgaaagatg
    32941 ctggcagcgc atcatcaagg ctttttcgtg tattcaggcg ttccagtccg gttttcagcg
    33001 cttccttggc ccgctggtac aaatcaattt cctttggcgt gatcttgccg gtttcgaaag
    33061 gcagaacgtc ctgcgggtaa tgcgctgact gtttttgctg caaacgctgc tgttccgcca
    33121 gtttcgtgcg gatggcgcga tcctggtcgg cgatttcctg gcgcgctgct ttttcgcttt
    33181 ctggcaggtc atttccgacc tgtgcggcct ggagtgttgc cgactgtttc tggctgactg
    33241 cttccttgcc ccgccacccg aacttcatga cgaagacacc aatcattccc gctatcatga
    33301 atattccgag catgatgacg acatgccggc tcagcttgcc tgatcgcgcg gcattcaatt
    33361 gaaccaccac gattgtcttc tgctcgtctt gtctgattta agacggacgc gcacctcatc
    33421 atcgccaagc ttgagcagcc aggcattcgc cgtacgctgc gccttgatgg tctgattctg
    33481 gtctaccgta taattgacga gctccggctt gtcatccgga tcaagcacga acagcgcggg
    33541 caactcctga atgcctggcg gcaggcgaaa ccaggtgaaa cggccatcgt cagcgaccag
    33601 cgtcggggaa aaatccgcct tccccgtaat ttcatagttt acaaacaggt tttccagcgc
    33661 cgggctatcg ggggcatctg tggcatcggt tgtcgatgtc gcagcgctgg agtcgttacc
    33721 cgcggtctcg tgaccggcgt catcgtacat atccggcaac gtagcgatgc catcctctgg
    33781 cacgctccag cgtacgcgct ggtaccacac gccagagacg gccgattcga atgtcagcag
    33841 atatgaccgt ttgtcggtaa tcagtgttgc actgttgctt ccccccgcct gcatcggcat
    33901 gatgaaaatg tgacgcttgt cacccgacac gatatagggc cagccccgtt tttcgtccgc
    33961 cgaaggaaac caggagacga tatgttcgcc ctccggtacg gcaatttcgg tgaacgaatg
    34021 cgcgcgcgcc ctgacgggat aactgcggtt gggatcgtac tcataggtga ccaggcgcgc
    34081 atcggcagac agactgtcag caaccggacc gtacgcgtca acggcagaaa cggggtgcgg
    34141 aggaagcggt atggctgttc cggccacaac aaacggcgcc ggccacaacg atgccagaca
    34201 agccaggcac tgataacgca gcgggctcat tggccaagtt cctcgtcaat ggcaaactgc
    34261 gtgacataga agcccatcgg gttgcgcaac gcttccgcct cgctgtgcac gggctcgagc
    34321 gcataatgca gcgtaaccgc tttggtttct gtttttgtca caccgctgtt gctcagtgtg
    34381 gacagacgga caatgatggt gagcacattc tgggcgctgg gcagtatggt gatgcgctgc
    34441 aactccacgt tgcgctcaag tgacggatcc cgggccatgc gttccagcgt gtcatcgctg
    34501 acaaggaatt caccgacttc ggccttggcc ttgcccaaca cctgggtttt gacatccggc
    34561 aggaattcgt tgcgggtgcg gtatggatcg atggtgagca gttcacgcac gaacttcgct
    34621 gcaaaatact tgatgttccg tgcatccggg gtgaatttct gcgcaacggt tgtgctgaca
    34681 gccacctgcc cggaatcccg gtccgtactg acgaaatagg ggacccgctc cttgagcggc
    34741 atcattgagg cgatggcgaa cccaagcgcc agattcccca ccatcgacat tgcgagcaac
    34801 actgccatgc ggttggcttc aagacgcggt tcgagattgc gtttcaactg gttgtacagc
    34861 aattcatcca tgacctgttc catccgactg tttggtgacc ctgcacaccg caagtttcac
    34921 cgggcacgtg ccgatccagc ccggcaccct gaagacaggg cttcgcctgc acggcaggta
    34981 tgccagttgt ttacaacaga ttataatcac aagtgcgatt gcttataaat gaaattttca
    35041 tgacaacagc gcgcccaggg cagcagcctg tcgcgtctgt cacggaatat cgggaaaaag
    35101 actaggaaat ctcggcgggg aaatacaccg tcatgcgtgt gccgcgtcca ccgggaggcg
    35161 tggagatcac gaggcgagcc tggtgctggc gcacgatttc ggccactatg gacagcccga
    35221 ggccacagcc attccccgaa ctgccctcga tgcggtaaaa acgttcgagc actttgtcgc
    35281 gctggttctc agggatgccg acaccattgt cttcaacttc aagtaccggc gtgtcaccat
    35341 catggcagcg caccgtgatc tcgccttcct gctcggtata acgtagcgcg ttgtcgacaa
    35401 gattggcgat catctctccc agcagcagtg gatcgccggc aatctgtaca ggctcaagct
    35461 cgaatccgag atcgatgttc ttgacgcgcg cctgcgccag ccagcccgcc atacccgcct
    35521 cgatgaggtc ggcaaggttg accggctgca tgttgagcag gtattgcgca ccgggttcgg
    35581 cgcgtgccaa cgccagcaac tggttggcaa ggtgggtggc tcgacgtgtg gcaccaagca
    35641 gcgtatcaag cacctgtctc gattcgttgg cctgcggctg acgcagcagc agttcgacct
    35701 gggtttgcag gccggccagc ggcgtgcgca actggtgagc ggcgttggca aggaagcgtt
    35761 gctgaccttc gatcaccgag gacagttcgg ccagcaggcc atttaacgaa gcgatgacgg
    35821 gacgaacttc gagcggaaca tgatcttcgg gcaccggacg cagatcacga tgcgaccggg
    35881 ccgcgatttc acgttgcagt acggccagtg gtcgcaggcc gcggctgacg ccatgcgata
    35941 ccagcaccac ggccgccaat gcgatcacca gcaccggcga gatcatgagc gccatcagtt
    36001 cgtagaggtt gcggtcgcgc ctgacgagcg tctcggcgat ctgcatgacc acatagcggg
    36061 gacggtcttc cgggcgttcc tcattgtcga gcgccacgcg catggcgccg acgcgcacgg
    36121 cataaccctg gtagaccgcg tcgtagaaca ccggacggtt acccagacgc cgtggcaggg
    36181 gcaactgcag gtcaccggca aacatgtgcc cgctgtcgtc gcggatctgg aaccaggcaa
    36241 tatcgtatac gtgcgtgaac agcgaacgtt cggagtcgac agacatgcgc agcaccgggt
    36301 ggccatcaac cacggcgatg cgctgtctca atgcctggat gggatcgagc agcgcccggt
    36361 cgaacgaccg cgtggaggcc gtacgcgcgc tgtagaaaga tgccatggtg gccacggcca
    36421 ccagcgccag cagcggaacc agcagcgatc ccagcagcag cccgcgcagg ctgggaggtt
    36481 tcccatgatc tgtgcctctt accagttgcc ggacggccgc gacacactgg ttccacacgc
    36541 cgttcatgcg tggcacagac aatcagtgtt ccgtctgcgg cacaccttcg ctggcttcga
    36601 gcaggtaccc aaagccgcgg atagtgcgaa tattgacacc agccgattcc agctttacgc
    36661 gcaggcgcga catgtagacc tcgatggcgt tcgggctgat gctctcatcc cagttgcaga
    36721 cggcctgcag aattttctcc ttgctgagca cctgcccctg acgcaacagc aggtattcga
    36781 gcactgccca ttcgcgcgcg ttcagcatca gctcgttgtg gc
//
LOCUS       DAIANL010000107        14462 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2786248, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000107 DAIANL010000000
VERSION     DAIANL010000107.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 14462)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14462)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14462
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2786248"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3034 a         4769 c         3833 g         2826 t
ORIGIN      
        1 acaaactttc cagcgatgca ttcatatctg tcctgaccct tgccgcactg tcagcgggcg
       61 atggtattgg tgcgcttgat cttgttctgc aactggatga ttccgtacag caaagcctcc
      121 gctgtcggcg gacatcccgg cacgtaaata tccactggta cgatgcggtc acaaccgcgc
      181 acgacggaat aggaataatg atagtagccg ccgccattgg cacacgaccc catcgagatc
      241 acccagcgcg gctcggccat ctggtcgtac accttgcgca gggccggagc catcttgttg
      301 cacagtgtgc cggccacgat catcacgtcc gactgccgcg ggctggggcg gaacaccacg
      361 ccgaaccggt ccaggtcgta acgcgatgca cccgcctgca tcatctccac cgcacaacag
      421 gccagcccga acgtcatcgg ccacaatgag cccgtgcgca tccagttaat gagcttgtcg
      481 gcggtggtgg tgacgaagcc ctgctccaaa acgccttcta ttcccattcc agcgctcctt
      541 tcatccattc gtacacaaat ccgaccacaa ggatgccgag aaacaccagc atggccagat
      601 aaccgaacca gccgatttca ttgagcacga ttgcccaggg gaacaaaaac gcaatttcca
      661 gatcgaagag aatgaacagg atcgccacga ggtagtagcg cacatcgaat ttcatgcgcg
      721 catcctcgaa tgcctcgaac ccgcactcgt atggcgacag ctttgcgctg tcgggatggt
      781 ttggcgccag caaccacccc atcagcatgg gaaccacacc aatgagcaag ccgacgagca
      841 cgaacagcaa caccggaaaa tatccgttca acatgattca ccccccatgt aaagcgaccg
      901 ccattccgca ccgcgccagc ggccgccctg cacgatgcgc gaccggcatg ccagccggcc
      961 attcgcgcgt agcccattca atcgtaattc tcgccaccag cacggacgcc ggcactacgt
     1021 gccgcacgat ccggagttcc ggcaactact tgacatattc ctgacaaact cacgcccgga
     1081 gccgggcgca caacgccttt tgctcctggc ttgccgacgc acgggttgtg catccggcag
     1141 cctgcccgct tcttgcgatg cggcttgcgc gcgatgtaag cgcgaacccg ctcgattact
     1201 ggtgccgacg gcgagactcg aactcgcaca gcttgcgcca ctaccccctc aagatagcgt
     1261 gtctaccaat ttcaccacgt cggcatgctg acggaacgga aggataaccc gtcccggcgg
     1321 caatatgata cccgctactt ggggatctca ccggcttttg accggtcatt gcctgaaact
     1381 gcctgacccg tcaccggcgg catgctggcc ggtactaccg gcttggccag ccgcgtctgc
     1441 accggcggga gcgtctgcat gacgcccgcc gtcgcatggc ttcgacctga aaaccaggtc
     1501 aagaccagac tggtcacaaa aaatatcgct gccagcagcg ctgtcgtcct gctcagaaag
     1561 tttgccgacc cggaagcgcc aaacaggctt ccagaagcac cactcccgaa ggcggccccc
     1621 atatcggctc ccttgccctg ctgcagcagc accagcgcga tcagcgccac cgttaccagc
     1681 agatgcagga tcaaaataag cgtttccaaa ttctagtcct ttatgccacc ctgctcaagg
     1741 ccgggctgcc ctgcaaatcg caatgaattc ttcggccacc agcgcatccc gttcgcgcaa
     1801 gcggttacga atgaacgcat gcaccgcctg tgcctgctcc ggcgtcgctg tgcggccggt
     1861 accgatcgcc cataccggct cataggccac cacggcccga ccgaacgctg cgataccggc
     1921 cttgtccagc acggcatcga tctggcgcgc gatcaccgct tcagtcactc ccgcctcacg
     1981 ttcatgcaac gtctcgccga cgcacagcac cggaaccagt ccggccgcca ctgctgcagc
     2041 aaacttggcc gccgcagcgt gatcgctttc gccatagagt gccctgcgct ccgaatgccc
     2101 gatcagcaca tgacggcagt cgaaatccag cagcatcgat gctgacactt cgccagtata
     2161 ggcgccggaa gcatgcttgc ttacattctg cgccccccag gcgatactcg atccggacag
     2221 catcgactgg gcctgcgcga gatatggtgc cggcacacac actgacacct cggcaccctg
     2281 ccaaccgtcc agctcgtcgc gcactcggcc gagcaggatg cggttcgact gcaggctgcc
     2341 atgcattttc cagttaccgg ctaccagctt ctgccgcatc acataccccg ccatccaaat
     2401 cgtgccatgt tacacaaaag catcacgttt ggtcaatcct tgcaaaaagt ggtaatcttg
     2461 cgccgctcgc atgtgactcg ctcgcgacca tcatcgcagc gcgcacacga ctgccgcggc
     2521 acgaccgctt ggtcatggcc gctgtaacgg atttgcaata tcagcaggat atcattccga
     2581 ctgcgttcga gtgtctggcg cctatccagc gcgcaccccg ctccgcaacc ggtttaccgg
     2641 accaggctgc agctgcccgg accaacatgt ccggcagctc cgttaccatc attggagaaa
     2701 ctgatgacca agcaattgaa aattcgcact cttgccgcca cgctgctgct gggcaccggc
     2761 ctggcctttt cggcagctca tgcatccgca ggggaaatca ctggcgccgg atcgacgttt
     2821 gcctacccga tctacgccaa gtgggccgaa gcctacaaga ccacgagcgg catcaacctc
     2881 aactaccagt cgattggttc cggcggtggc attgcgcagg tcaaggccaa gaccgtggat
     2941 ttcggcgcgt ccgacatgcc gctcaagccc gaagcgcttg agcaggccgg cctgacccaa
     3001 tttccaaccg tcatcggcgg tgtggtgccc gttgtcaaca ttccgggcat cgcgccggga
     3061 caactgcacc tggatggcgc cgtactggcc gacatctatc tgggcaaggt ggttacctgg
     3121 aacaaccccg aaatcgctgc gctcaacaag ggcctcaagc ttcccgacac gcatatcact
     3181 gtcgtgcacc gctccgacgg ctcgggcacc acgttcatct tcaccaatta cctgatcaag
     3241 gtcagcccca cctgggctgc caaagttggc aacgacacct cggtggcctg gccgaccggt
     3301 gtgggtggca agggtaacga aggtgtatcc tcgttcgtcg agcgcatccg cggttccatc
     3361 ggctacgtcg agtatgccta tgccaagcaa aacaagatga cctacgcgct gatgaagaac
     3421 catgatggca agtatgtgcc accgttctac gccaacattc aggccgctgc agccggcgct
     3481 gactggatcc atgcgcgcga cttctaccag atcctgacca acgagcctgg caagaacagc
     3541 tggcccatct ccggcgcgac cttcatcctg atgcacgcga ccgaggacaa gccgcagaat
     3601 gccaaagccg tgctcaagtt cttcgactgg tcattcaaca atggctccaa gctggctgaa
     3661 ggcctcgact acgtgtccct gcccaagtcg gtgactgacc tgatcgaaaa gaactggacc
     3721 agcaagatca aggaccccag tggcaaggcc atctggtaat cgtcacacgg acaccgtccc
     3781 tcggcacgga agggaatccc ttccgtgctt ttgcacgtaa atcagtctga tttcccatga
     3841 ccccaccatc cagcgaaaac cgcagtcgca aatttgccgt acaggacacc ctgtttgtca
     3901 acgtcaccag actgtttgcg gttatcgtgc tcgtgctcct gcttggcatc atggcctcgc
     3961 tcgtgatcgg cagcctgccc agcatacgga aattcggttt ccacttcata ttcagccagg
     4021 actgggaccc cgtcagcgga aaattcggcg ccgtggttcc catgatcgga acgctcgcca
     4081 cctcattcat tgctctgctg atcggcgtac ccatcagttt cggcatcgcg ctgttcctca
     4141 ccgaactgtc gcctaactgg ctcaaacgac cactcggtat cgccatcgag ttgctggccg
     4201 gaatccccag catcatttac ggcatgtggg gactgtttgt tttcgcccct tttttcggtg
     4261 accatatcga accgtggatc atcaacaccc tcggcaacct gccgctgatc ggcacgctgt
     4321 tccagggcgc tccaatgggc attggcatcc tgtccgccgg tctgattctg gcgatcatgg
     4381 tcattccgtt catagcatcc gtcatgcgtg acgtgttcga aatcgtcccc cccatgctca
     4441 aggaatcggc ctacggcctt ggcgcaacga cctgggaagt cgtgcgtttc gtggtgctgc
     4501 cctatgcccg catcggcgtg gtcggcagca tcatgcttgg tcttgggcgc gcgctgggcg
     4561 agaccatggc ggtcaccttc atcatcggca attcggacca gatcagcctg cctttttcgt
     4621 tactcaatcc gggcaacagc atctcgtcgg cactggccaa cgaatttacc gaagcgacca
     4681 cagaaatcta cacctcgtcg ctgatcgagc tcggcctgat cctgttcttc atcacatttg
     4741 tcgtgctgtc catttcgaaa ctcctgctga tgcacatgag caagcaggaa ggactgaagt
     4801 catagggccc cctcatgctg cctaaatatc agcgtcgccg cttgctcaac gctttcaatc
     4861 tgaccctgtc cctgatcacc atggtactcg ggctgacagc gctcggctgg attctgtggg
     4921 tgctgctcga cttcggcctc aagggaatcc acccatccat cttcacgcag atcactccac
     4981 cacctggcag taacggtgga ctgagcaatg ccattttcgg cagtgtcgcc atgtcgctta
     5041 tgggaacaat ggtcggcacc cccatcggta tcatggcagg cacctacctg tccgaattcg
     5101 gccagcgcgg gtggcttgcg cccgtcaccc gcttcatcaa cgacatcctg ctatcggctc
     5161 cgtcgatcat cgtcggcctg ttcgtctacg aagtgtatgt cacgaccacg caccactttt
     5221 ccggctgggc aggcacgttt gccctgtcac tactggtgat ccccgtagtc gtgcgcacca
     5281 ccgaagacat gctgcgactg atcccgaaca cgctgcgcga ggctgccgcc gcactgggcg
     5341 caccgcgctg gaaagtggtc acgctggtca tttggcgcgc ggcacgagcc ggcatgatca
     5401 ccggcgtgct gcttgccata gcacggatca gcggggaaac tgcaccactg ctgttcactg
     5461 cgctcaacaa ccagttcatg aacaccaaca tgaatacacc catggccaac ctgccggtcg
     5521 tcatcttcca gatggcaatg agtccctatg acgactggca caaccttgcc tgggctggcg
     5581 ccctgctgat cacggtcacc gtactggtac tcagcatcat cgcgcgcttc ctcttcaaaa
     5641 aagaagttac tgcaaactag gaatatcgag cgccccatgg aatcgaacat caaggtcagt
     5701 ctgcgcaaac tgaatttcta ctatggcaag gcgcatgcgc tcaaggacat caaccttgag
     5761 atcattgcca atcaggtcac ggctttcatc ggaccgtccg gatgcggcaa gtcgacacta
     5821 ctgcgcacac tgaaccgcat gtacgacctg tatccaggcc agcgggccga gggcgaaatc
     5881 ctgctcgacg gcatcaacat cctcgacagg aagaccgacc tgaacaaact gcgcgcccgc
     5941 gtcggtatgg tattccagaa gccaacgcca ttccccatgt cgattttcga caacatcgcc
     6001 ttcggcatca agctctatga gtcgccatcg cgctccgaac ttgctgaccg cgtcgaatgg
     6061 gcgttgcgca aggcggcctt gtggaatgag gtcaaggata aactggggca gagcggctac
     6121 agcctgtcag gaggtcagca gcaacgcctg tgcattgcgc gcggcattgc cgtcaagccg
     6181 gaagtactgc tgctcgacga accagcctcc gcactcgatc cgatctcgac cgcacgcatc
     6241 gaagaactga tacacgaact caaggccgat tacacgattg ccatcgtaac gcacagcatg
     6301 cagcaggcgg cgcgtgtatc cgactacacg gcgtacatgt atctgggtga aatgatggaa
     6361 tatggtcgca ccgatgtcat cttcaccaac ccaaccaaaa aagaaaccga agactacatc
     6421 accgggaaat ttggctgatg gccaccagcg tacccgttcc cggcaggcat gccataccgg
     6481 aaagctgaca acccgcacac cggcctcgcg cagtagccgt cacgccgggt ggtgggcgga
     6541 tgcaacggca cgaacagcgt cagcgatacg cgttgcttcg cgttttgcca cggcctcatc
     6601 ggcactttcg accatcacac gaatcaacgg ctcggtacct gatgcgcgca acaccacacg
     6661 cccggtcgta ccgagcacgg actcagcagc cgccacagcc gcgcaaatcg cttcatgttg
     6721 cagatcaatg cgctccttca tgcgtacatt gatcatcgtt tgcggataca tatgcagatc
     6781 ggcaacgtac tgctgcagcg acatgcccga ttcacgcacc gcccgcagca cctgcagcgc
     6841 agcgatgatt ccgtcgccgg tggtatgttt ttccagacac aacagatgcc ccgacgcttc
     6901 gccaccaagc tgccagcctt tttccgccag cagttccagc acgtagcggt cacccacctt
     6961 ggcacgccca aacggaatct cgagccgttc aagcgcatgc tgcagaccaa ggttggtcat
     7021 caatgtaccg gccacgccac ctgtcagttc gccggacaga tgacgatagc gggcgatgac
     7081 gtacagcagg tgatcgccgt tgtacaatgt accgtcagca gcagccatga tgacccgatc
     7141 gccgtcacca tcgagcgcaa tgccgtagtg cgccccgtgc tgcagcacgg cagcactcaa
     7201 ggtctccgga tgcgttgctc cgcactcatg attgatattg gtaccatcag gctgattgcc
     7261 gatggcgatc acttcggccc ccagttcgtg cagcaccggt ggtgccacat gataggttgc
     7321 accatttgcg caatcgacca ctatccgcag cccacgcaga ttgctttcaa acgggaacgt
     7381 acttttacag aattcgatgt agcgtgccgg cgcatcatcg atgcgtttga cacgccccaa
     7441 ctgtgacgat ggcagcgtaa gcagcggctt ctccagttcg cgctcgattt ccagttcggt
     7501 tgcatcggcc agcttttcac cactggctga aaaaaacttg attccattgt cttcgaatgg
     7561 attgtgcgaa gcggaaatca ctatacccgc ctgcaaccgc aaggcgcggg tgaggtacgc
     7621 taccgcgggt gtgggcatcg ggccgaccag acgcacatga acgcctgccg cacacaatcc
     7681 ggcctgcagc gccgcttcga gcatgtatcc cgatacccgg gtatccttgc cgatcagcac
     7741 tacagggtgc gctccggcag gcagtgagct cctgtcgctg gccagcacct tgcctgccgc
     7801 atagcccagt ctcagcgcga aatccggtgt gatcggcggt tcgccaacct tgccgcggat
     7861 accgtctgtc ccgaaatact gcctgctcat tcgctctccc cgattgcctg ccaaaccagt
     7921 atggcatccc gcatggccgc cacatcatgg acacgaaaca gactggcgcc ctgtcgcagc
     7981 gcatacagat gcgccgccag tccggcatac tcacgctccg cgaccggacg tccggtcacc
     8041 tgcccgagca tggatttgcg cgacatgcct gccagcagtg gcttgccgat cagcttcagc
     8101 cgccccagat gtcgcagcag ttgcagattg tgcgccagcg acttgccaaa accaaagccg
     8161 ggatcgatga cgatactctc ggatgcaatc ccggcctgct cggcgagcgc cactttgctc
     8221 ctcagaaact gttccacttc gtccacgaca tcaccataca ccggatgctg ctgcatgatg
     8281 tccggctgtc cctgcatgtg catcaggcac acggcaacgc cggtattcgc cgccagttcc
     8341 agtgcaccgg gtgctgcgag ggcttcgaca tcgttgatca tgtctgcgcc agcctcgatg
     8401 gccgcacgca tgacgcccaa atgccgggtg tcgaccgaca gtacggccga acacccacgc
     8461 agcgcctcaa tcaccggaac aacgcgcgac aactcttcat cgacactgac tggctgtgct
     8521 cccgggcggg tagattcgcc accaatgtca atcacctcgg caccttcatc cagcaaacgc
     8581 agggcgtgag cgatagccgc ccgggcatcc ggcaggttac cgccatcaga aaaggaatct
     8641 ggcgtaacat tgacgatgcc catgatgcgc ggcacacaca aatcaagtat cttgtcgcgg
     8701 caatgcaggt tcatggcgtt aacggcagtc gtaggttcgg aaaaaccggt gaattcgctg
     8761 gagcctgggc acttgtgacc agccacagtc agtctgccgg agtgcgggcg tttacgtaaa
     8821 aaaactcagg ccggtttccc ggcctgattg catcatgccg cggcattcac acgccggcag
     8881 caggggtgat tgtcgacgca gcgcctggag gcgtattgcc acccgtcggg gttgcctgcg
     8941 acccgccacc cactggcttg ggtggacgtg gctgattgcc ttccataata tccctgatct
     9001 ggtcggagtc gatggtttcc cactcgagca aggcatgagc catgacttcg acacggtcac
     9061 ggtgatcttc aatcagcttg cgtgccagcg cgtactgctc gtcgatgatg cgacgaattt
     9121 ccccgtccac cttttgcatg gtggcttccg aaacgctttt gtgtgtggtc acgctgcggc
     9181 caagaaaaat ctcgccttcg ttttcgccgt acaccatcgg accgagcgct ggcgacatac
     9241 cccattgtgt caccatgcgc cgcgccattt cggtcgcacg ttcaaaatcg ttcgacgcgc
     9301 cagtggtcat gtgattcatg aaaatttcct cggcgatgcg cccaccgaac agtacggaaa
     9361 tgttttgcag caattgcgcc cgatccatgc tgtaacggtc ttcggtcggc aactgcatgg
     9421 tcaggccaag tgcgcgacca cgcggaatga tggtcacctt gtgcaccgga tctgtcttgt
     9481 cgagcagcca ggcaaccacg gcatgaccgg actcatggta tgcggtgttg cgacgctctt
     9541 cctccggcat gaccatcgtc ttgcgttcgg cgcccatcat gatcttgtct tttgcccgtt
     9601 cgaaatcgtc catatccacc agccgcttgt tgctgcgggc cgcgaacagg gccgcctcgt
     9661 tgaccagatt ggccagatcg gcgccggaaa acccgggagt accgcgtgca agaatgtcgg
     9721 cacgcacatc gggtgcgctt ggaatcttgc gcatgtgcac cagcagaatc tgttcacgac
     9781 cacgaatatc aggcaatggc accacgacct gacgatcgaa tcgacccgga cgcagcagcg
     9841 cagggtcaag cacatcagga cggttggttg ctgcaatcac gatcaccccg gcagtgccct
     9901 cgaaaccgtc catctcgacc agcagctggt tcagtgtctg ctcgcgttcg tcgttgccac
     9961 cgcccatgcc tgcgccgcgc tggcggccaa cggcgtcaat ctcatcgatg aagatgatgc
    10021 acggtgcatg tttcttggcc tgatcgaaca tgtcccgcac acgcgcagca ccgacaccaa
    10081 cgaacatttc gacgaaatcc gaaccggaaa tgctgaagaa aggcaccttc gcctcgccag
    10141 caatcgcctt cgccagcagt gtcttgcctg tacccgggct gccggccatc agcacgccgc
    10201 gcggcacacg cccacccagc ttctggaatt tggccggctc gcgcaggaag tcgaccagtt
    10261 ctgccacttc ttccttggcc tcatcgcatc ctgccacatc ggcaaatgtc acctgattgg
    10321 ccgactcgtc cagcatgcgc gccttgctct tgccaaacga aaacgcgcca cccttgccgc
    10381 caccctgcat ctggcgcatg aagaataccc acacaccgat gagcagcagc atcgggaacc
    10441 aggaaatgaa gatgttcatg aggaacgacg gctcctcttc cggcttggcc tcgatggcca
    10501 cgccgccctt gagcaggtcg gataccatcc acggatccgt gggcgcgtag gtgctgaagc
    10561 gccggccgtc ggtgcgcacg cccttgatca cgtgattttc gatcaccacc ttggcgacct
    10621 gcccctgctt cacctgatcg agaaactggg aataatccat ctgcgcctgc gtgctctggc
    10681 gggtattgaa ctgattgaac agcgtcatca gcaccagcgc caccacgagc cagatcgcta
    10741 tattcttgaa catgttattc atccgtatgc tccattcgag ccacttgcca cctggccaac
    10801 atttacctga ttctatactt gattcaatcg ctttgtaact gtctgcgcag atttttggct
    10861 aacagataca cctcgctgct gcgatcgcgt gaggccttcg gcttgcgcgt ccacacctgg
    10921 gtaaaggctg cacgcacggc tgacaggtac tcctggaacc cttctccctg aaataccttg
    10981 accagaaacc agccatcctc tttcagatga ctgacagcaa aatccagtgc gagttcgcac
    11041 agatacagac tgcgcgcctg atcggcactc gccacaccgc agatattggg cgccatgtcg
    11101 gaaataacaa ggtctgcccg ccggtttttc aacgccgagc gcaaccggtc cagcgtagcc
    11161 ggctcggtaa agtcaccttg caggaaggtg actccgggta gcggcgacat ttcaagcacg
    11221 tcaagcgcaa tgacttccgc accttcgcga ctggcgaact gcgaccagcc accgggtgtc
    11281 gcgccgaggt cgatgaccag actgccgcgc cggatcaggt gatcgcggcg gctgatctcc
    11341 tccagcttga acgcagcacg cgagcggtac ccctgagcct tggcctgttt gacataggga
    11401 tcgttgacgt gttcccgcat ccaggcccgg ctggtcttgg ccggtttcat gactgcctgg
    11461 ctcgaaccac cagcaccaac cccagcagac tttcgatcag atagacgata ctggcgatgc
    11521 catgccagcg agcaaagcgg acgctgaacg cgctttgcat cacttcgcgt ggaaacgcct
    11581 gcgctttcag actctcgatg actggctgga taccaaaata gccacccagc gtcagcagca
    11641 gcatgcacag tgtcgtccag aaaaacagtt gcctgagcgc agaggcacca aagcgcgcca
    11701 aacggtgaat caacagatag aacccgctga cgattcccac ccaggacatg atggagaaca
    11761 tggtgccggc gagatttcct gccagccgcg tatcacctgg cagcttccag aacagcaccg
    11821 gagcggccag atagccaatc gcccacaacc cgcctaccca ggcggtcagc atgacggctg
    11881 cgagcagatc gccgaaacgc gacatggtca gaggtactgc acgtccagca cttcgtactg
    11941 gcgcacacca ccgggcgcct gtacgtcagc ggtatcaccg gcatacttgc cgatcagggc
    12001 acgtgccagc ggagagctta tggaaatctt gaagtgcttg atgtccgcct cgtcatcgcc
    12061 cacgatctgg taggtgaccg tgtcaccgga ttccatgtct tcgagttcga cggtggcgcc
    12121 gaacaccacg cggccttcgg cctcgatgct cgccggatcg ataatctgcg ccgtggacag
    12181 tttggcctcg agctcggcga tgcgcccctc gatgaagccc tgcttttcct tggccgcatc
    12241 gtattccgcg ttctccgaca ggtcgccctg ggcacgcgcc tcggcaatgg cggcaatcac
    12301 cgacggacgc tcgacggttt tgagacgatg caattcttcg cgcagcagtt gcgcccccag
    12361 caccgtcaat ggaatcttgg ctatcgaatt catgttccac tttcccgggc ggccaaccgc
    12421 ccatgcaatc cttgcaaatc gtacacttcc agcccgccca tgccacgcat accgatacac
    12481 gccgcccgcg caccggccag cgtagtgtag taagtcactc gctgcgccag cgccacacga
    12541 cggatctcga ccgaatcgcg caccgcacgc ttgtcatcga ccacgttgac gatcaggctg
    12601 atttcattgt tcttgatcat gtcgacaata tgcgggcgac cttcggctac cttgttgaca
    12661 actgttacct gcagcccgcc ggattgcaac gcggccgccg tgccgcgcgt ggcgaccagc
    12721 acaaagccca gctcgatgag ctcggcacca atttcaacga tacgttcctt gtccgcattg
    12781 cgcacgctga tgaaggcatg accgccacgt ggcagcttga ccccggcagc aagctgcgac
    12841 ttgacgaacg cttccgcaaa actcatgccc acgcccatga cctcgccggt ggatttcatt
    12901 tccggcccga gcagggtatc gactccctga aacttggcaa atggaaacac cgcttccttc
    12961 accgaataat agggcgggat gatttctctg tccacgccct gctgcgccag cgacatgccc
    13021 gacatgcagc gcgcggccac tttcgccagc ggcacccccg tggctttgga gacgaacggc
    13081 acggtgcgcg acgcgcgcgg gttgacttcg agcacgtaga ccgtatctcc ctgcagggca
    13141 aactgcacgt tcatcaagcc gaccacgttg agggcacgcg ccatggcaac ggtctgacgg
    13201 cgaatctcgt cctgcaccgc agcgctcagg ctataaggcg gcagcgaaca tgccgagtcg
    13261 cccgaatgca cgccggcctg ttcgatgtgc tccatgatgc cgccgatcac caccatctgc
    13321 ccatcggaca ctgcatcgac gtcgatctcg atcgcgtcat tgagaaaacg gtcgagcagc
    13381 acaggagaat cgttggacac cttgaccgcc tcgcgcatgt agcgctgcag gtcttcctcg
    13441 gcgcgcacga tctccatcgc ccggccgccg agcacgtagg acggccgtac caccagcgga
    13501 tagccgattt cggctgccag cgcaagggct tcgtctgccg aacgcgcggt gcggttgggt
    13561 ggctgcaaca agcccagccc gtgcagcatc tgctggaaac gttcccggtc ttccgcgcag
    13621 tcgatcatgt ccggcgtcgt gccgatgatc ggtgcgccac aggcctgcag atcgcgcgcc
    13681 agcttgagtg gcgtctggcc gccatactgc acgatcacgc cccacggacg ctcgacgcgc
    13741 acgatttcca gcacgtcctc cagcgtcagc ggttcgaaat acagccgatc ggacacatcg
    13801 taatcggtcg acaccgtctc cgggttgcag ttgaccatga tggtttcgaa accgtcttcg
    13861 cgcagtgcca gagcagcatg cacgcagcag taatcaaact cgatgccctg gccgatgcgg
    13921 ttgggcccgc caccgagcac catgatcttg cgccggtcgg tgggtcgtgc ctcgcactct
    13981 tcctcatacg ttgaatacat gtacgcggta tgagaagcaa attcggctgc acaggtgtcc
    14041 acccgcttga acaccggatg cacgcccatc gcccaacgcc gttcgcggac ggagtgcttg
    14101 tcgctaccca gcagagttgc caatctgcgg tcagaaaaac cattacgttt gtagttcagc
    14161 agcgtcgccg catcgagtga ttccagcgag cgccccgcga gcgcagcctc atccgccacc
    14221 agcgcctgga tctgcaccag aaaccacagg tcgatgcggg taaaatcgta gacttcctca
    14281 agactcatgc cgatacggaa cgcatcggca acataccaca gacgctccgg tcccggattg
    14341 ccaatctcgg cctcgatcac gctgcgctcg cgcgtgagcg gatccaggcc atcacgaccg
    14401 gtttccaggc cacgcaacgc cttctgcaac gattcgcgga acgtacgccc gatcgccatc
    14461 ac
//
LOCUS       DAIANL010000108         5888 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3203062, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000108 DAIANL010000000
VERSION     DAIANL010000108.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3203062"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1075 a         1660 c         1968 g         1185 t
ORIGIN      
        1 ccgtgcgcgg gtcgatgatg gcttcaacca gtcgcgggcg ccataccgtg ccgccgttgg
       61 ccagcgtcgc cgtcgcgact gcgagctgta gcggcgtggt cagattgtat ccctggccga
      121 tgccgcagat gacggtttct ccggggtacc acggctggcg tgagatgtgc gccttccatg
      181 cccgtgatgg cagcacgccc gccagttcgc cgttcatgtc gataccggtc ttgcggccaa
      241 aaccgaattg ctccagatag tcatgcatgc ggtcgatgcc cagatcgttg gccaggcgat
      301 agtagtaggt gtcgcatgac actacgatcg agcggtgcat gtcgaccatg ccgtgaccgt
      361 tgggtgccca gtcgcggtag cggtggctgc tgccgggcag ggagaaaaat ccggggtctg
      421 caatcgtgtc ctgcggggtg cgcactccgt agtgcagccc tgccagtgcc atgaacggct
      481 tgatggtcga accctccggg taggcgccgc gcagcgcgcg gtcgagcagc ggacggtcag
      541 gcgaattgtt gagcgcatcc cagtcggtgt tgtcgatacc atcgacgaac aggttgggat
      601 cgaattcggg gtggctgacc agtgcgagaa tttcgccatt gcgcgggtcg attgccacca
      661 gcgcgccgcg atgctcgccg aatgcctgtt cggcgatggc ctgcatgcgc tcatcgatcg
      721 acagtaccag atcgctgccg gcgacaggtt cgtgccgtgc cagggtgcgt acggcatgtc
      781 cgccggcatc ggtttccatg atctccgatc cggtacggcc gtgcagcaac gattcgtagc
      841 tttgttcgat accggttttg ccgaggtggt cgctgccgcg gtagttgtca tattgtccgc
      901 tggcttgcag ggcacggatg tcggcatcgt tcatgcggcc gatataaccg atcaggtgcg
      961 cggccagcgt gccgtgtggg tactggcgga acaggcgtgc cttgacttcg accccggtca
     1021 gacggtaacg gttggcggcc acacgagcca cctcctggtc gctgagtcgg ttgcgtaatg
     1081 gcaaggtttc gaaattgcgg ctttcggcca gcagtttgaa gaagttgcgc cggttggccg
     1141 gggtgatttc aaccagttgt gccagctggt cgatggtggt gttgaggttg gaaatttttc
     1201 ccggagtcag ttcaaggcta taggcggaat aattgtgtgc cagcacgacg ccgttccggt
     1261 ccatgatgat gccgcggtta ggtggcacgg ggatcgaggt gatgcggttg ttttcggcca
     1321 gggtgtggta gtaggcgtaa cggtagatct ggatgtagcc cagacgtatc agcagcaaca
     1381 ggaacgcgac ggccaccaca ccggccgcga caaggatgcg ctggcggaag cccgagacct
     1441 cttcctgacg ggggcgcagc tcgatgggcg ctttcattgc ggcgccggtt gtgtgggggt
     1501 acgcagccgg tgcaacagaa cccagagcgg gaaccagcac agtgcgccgc tgagcacgga
     1561 aacgaaatag cgcggcgcgt tgcgggtgtc gccgagaaaa gtggctacca gcacgtcgat
     1621 gatctgttct gcaagcagga aggtcagaac aggcagggct tgctgccagg gcgggaagtt
     1681 atacagtcgg cgctggaaat gaccaagaat gtagatgctc actgcgtagc ccagcgcatg
     1741 ctggcccagg tgggcgccgt cgctcaggtc ttcgagcagc cccagccacc atgcggcgcc
     1801 gatgccgatg gcagtaggct gatagatggc ccagtacacc agcgtcagca gcaggaaatc
     1861 cggccgcagc cacaaccagt ccacctgcca gggcaacagg ttgaaaagcc agccggacag
     1921 caggctcagg gcaagcacaa tgctgctgtg gcggatcggg atggcgggac cttttggggg
     1981 tggaaaggcc atgacctcaa cgtaccggag tgtggtggat gatgagtacc tgtcggttgc
     2041 ggtcgatggc ggcgaccggt cggcagatca cgttgtcgaa ttcctggcct gctccggcgt
     2101 tgatatgctg gatgcgcgcg acaggcaagc cgggcggata gagcccgtcg aggccggagg
     2161 tgaccagcag gtcgccgttg cgaatgtcgg tactgtgagg cagatagcgc agttccagat
     2221 aatcgtcgga accggtaccg aacaggatgg catgctggcc agtacgagcg atggtgattg
     2281 gtacggcctg ttccttgtcg gtgagcagcg taatctcgct ggcagcgggg tagacgcggg
     2341 tcacctgtcc cagcaggccc tgcgcgtcga tcaccgcaga accgccttgt atgccttgta
     2401 tgctgccggc atccacggtg aacagccgac gccatgggtc gcgtggaatg gcaatgattt
     2461 cggcgctggt gctgggcagc atgacacggg cacgcaagcc gagcagcgcg cgcaatgtgc
     2521 ggttttcctg ttccagctgg tggttgtgct ggcgttcaag atcccaggcg atggactggg
     2581 cgcgtaatgc gtggttttcc tgtaccagtt cggcctgacg gacaaaaaac gcgtccatgg
     2641 ttcgccaggc gagccagggc gcctgcacgc cctgttcgat ggggacgagc agcaggttaa
     2701 aaccgccgcg tacacggtca agcgcgcgat aatggctgtc cagagtcagc agaacgatac
     2761 tgagcaccag atagatgagc agcttggcac ccgcgttcag gccatgggga aacagggcga
     2821 gatgagggtg cgctactgcc atggcggata gtggaagtga tcaggagtcg tgggcaaaaa
     2881 ccgtctgcag atggtccatt tcctcgagtg cccggccgga accgcgcacc acacaggtca
     2941 gtgggtcttc ggcgatgatc accggcaggc cggtttcttc catcagcaag cggtcgaggt
     3001 catacagcag ggcgccaccg ccggtcagca ccatgccttt ttcggcaatg tccgcgccca
     3061 gttcgggcgg tgtctgttcg agcgcggatt tgaccgcgct gatgatgttg ttgagcgggt
     3121 cggtcagtgc ttcgaggatc tcgttgctgg agatggtgaa gctgcgcggg atgccctcgg
     3181 ccaggttgcg gcccttgact tccatttcct tgacattggg gccggggaaa gcggtaccga
     3241 tgcctttttt gatggcttca gccgtggcct cgccaatcag catgccgtaa ttgcggcgga
     3301 tgtaattgat gatggcctcg tcgaacttgt cgccaccaac gcggaccgaa ttggcgtaga
     3361 cgatgccgcc gagcgagatt acgcccactt cggtcgtgcc gccgccgatg tcaaccacca
     3421 tcgaaccggt ggcttcggcc accggcaggt cggcgccgat agccgccgcc atgggttcct
     3481 cgatcagata cacctgacgc gcgcccgcgc ctatggcaga ttcgcggatg gcgcgccgtt
     3541 ccacctgggt cgagccgcat ggcacgcaga tgatgatgcg cgggctgggg tggaacatgc
     3601 gggtgttgtg gattttcttg atgaaatatt tgagcatctg ctcggtgatg gtgaagtcgg
     3661 cgatcacgcc gtccttcatc gggcggatgg cggtgatgtt gcccggtgtg cgccccagca
     3721 tctgtttggc ttcgaggccg acagcctgga tcgtcttttt ggagctgttg gcatcctgac
     3781 ggatggcgac caccgaaggt tcgtcgagca cgataccttt gccgcgaacg taaatcagcg
     3841 tgttggcggt gccgaggtcg atggccaggt cggtggaaaa gtagccgcgt aatgatccga
     3901 acatggtggt tgcattcgat ttgagtggtg aagatgacgc tcgtctgccg gcaacaaacc
     3961 aggtggcatt cgctggcggg cagaaacccg ttatgatacc ctattacact tgttttctgt
     4021 gaagaattgt acaatcatgt ctttgtccct gcaggatgtc gaacgcgtcg cccgactggc
     4081 gcgcattgca gtgactgctg acgaggcgct ggcctggcag cagcagctca acggagtgtt
     4141 cgggctcatc gccagcatgc aggcggtgga cacgcatgac atcatgccca tggcgcatgc
     4201 actcgacgtc agtcagcgca tgcgtgagga taccgttaca gaatgcgatc agcgtgaaaa
     4261 gttccttgcg cttgcgccgc aatctgcaga agggctgtat cttgtgccgc aggtaatcga
     4321 ataaatgagc gaagccaccc tgaagcaatt gtccgggcag cttgcgcaag gcacgttgtc
     4381 cagtgtcgag ttgacccgtg attacctgac tcgcatcgag cgcctgaatc ccgcagtcaa
     4441 tgcgttcatt accgtcaatg ccgatgtcag tctcgccgag gctgccgcag ccgacgcgcg
     4501 tcgcgctgcc ggtcaggcgg gcccgctgac cggcattccg gtggcgcaca aggatatttt
     4561 ctgcatcgac ggctggcgca ccacgtgcgg ctcgaaaatg ctggcagagt tcgtcgcgcc
     4621 gtatgatgcg cacgtcatcg gccggtgcag gcaagctggc atggtgacgc tgggcaagac
     4681 caacatggat gagtttgcca tggggtcgtc caatgaaact tcgtattttg gcccggtgag
     4741 gaatccgtgg gacatgaatg cggtacccgg cggctcttcg ggcggctcgg ccgctgccgt
     4801 ggctgcccgt atggctccgg tggccaccgg tactgatacc ggcggctcga tccgtcagcc
     4861 ggcagccatg accggcatca ccggactcaa gccgacctac ggggtggtgt cgcgttatgg
     4921 catgatcgcc ttcgcctcca gcctcgacca ggcgggaccg atggcgcgca ccgcagaaga
     4981 ttgcgcctgg ctcatgaacg tgatggcagg tttcgatgtg cgcgattcca ccagcatcga
     5041 acgtgcggca gaagactaca cccgcctgct cgggcagccg ctggccgggt tgaggatcgg
     5101 cctgccgcgc gaatattttg ccgagggttc cagccccggc gtcatgcgcg cggtggaggc
     5161 ggcgctcgac gagtatcgcc gtctcggtgc gcagaccgtc gacatctccc tgcccaacac
     5221 ggcgctgtcg attccggtgt attacgtgct ggcgccagcg gaagcctcgg ccaacctgtc
     5281 acgcttcgat ggcgtgcgct acggctaccg tgccgcgcaa tatgacgact tgaccgacat
     5341 gtatgaacgc acgcgggccg aagggttcgg agcagaggtc aagcggcgca tcatgatcgg
     5401 cacgtacgtg ctgtcgcatg ggtactatga tgcctactat ctgcaggcac agaagatccg
     5461 ccggctgatc gctgccgatt ttgcgcgcgc gttcgagacg tgcgacgtca ttatggggcc
     5521 gacctcgccg gacaccgcat tcgacttcgg cgcgcgctcg caggacccgg ttgccatgta
     5581 tctgtccgac atttatacga tcgccgtcaa tctggccggt ctgccgggca tgtcggttcc
     5641 atgcggcttc gatgcacgta accgcccggt cggcctgcaa ctgatcggca attatttcag
     5701 cgaggcccgc ctgctcggcg tggcgcatca atatcagcag gcaacggatt ggcatgcgcg
     5761 catgccggct ggtgtggcct gaatacgagc ggagtgtcgc catgcagtgg gaagtcgtag
     5821 tgggtctgga agtgcacgcg cagctgtcga cgcgctccaa gatattttcc ggggctgcga
     5881 ccgttttt
//
LOCUS       DAIANL010000109        12144 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_138532, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000109 DAIANL010000000
VERSION     DAIANL010000109.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138532"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2145 a         3425 c         4064 g         2510 t
ORIGIN      
        1 ggtcatggcg ccatcgccga tgatggcgat ggcgcgccgg tcgagcccct gcagccgcgc
       61 ggcagtcgcc atgccgagcg cggccgagat cgatgtgctt gaatggccgg tgccgaaggc
      121 atcgtacgga ctctcggcgc gacgtggaaa tccggcgatg ccgccatgct ggcgcaggcc
      181 gcccatggcc tcgcggcggc cggtgaggat tttgtgcggg tagctttggt ggcccacatc
      241 ccagatgacg cggtcgtccg gggtgttgaa cacatagtgg atggcaatgg cgagttcgac
      301 cgcgccgaag ttggatgcga aatgcccgcc ggtgcgggcg atgctctcca gcatgaaatc
      361 gcgcagcgac tgcgcgaccg tcggcaggtc ttcgcgcccg agtgcgcgca ggtcggcggg
      421 tgattcaatg gtgtcgagca agggataggg cgaactcaag atgttctctg caggatgaaa
      481 tgggccaggt cggccagccg gtcactgccc ggcagcttcg caattgcgtc gatggcctgc
      541 tggtgcaggt cggtcgccag ttctcgcgct gcagcgatgc ccatcaggct gacataggtc
      601 ggtttgtcat gctcggcatc cttgccggcg gttttgccca gctggtggct gtcgccggtc
      661 gcgtcgagca catcatcgac gacctggaaa gccaggccaa cgcaatgtgc gtaacgtttg
      721 agtgcgttac tgtgctccgg gttcaattcg gtgccgcaga gcgagccaag ctgtaccgcc
      781 gcgctgatca gggcgcccgt cttgaggatg tgcatgaact cgagttccgg tagcgtgaga
      841 tcgacaccga cgctaccgag atcgatggcc tgcccgccgg ccatgccgaa cgagcccgag
      901 gcatgtgcca ggatgcggat catctcgatc tgtcgtgcag ggtgctcacc cggagtgtcg
      961 ctggcaaggc attcgaaggc gaggctctgc aggctgtcgc cggccagcag cgcggtcggc
     1021 tcgccaaagg ccacgtgaca ggtcggtttg ccgcgacgca gcacgtcatt atccatgcag
     1081 ggcagatcgt catgcaccag cgaatacacg tggatcagtt ccagcgcgca tgcggcgttg
     1141 tcgacatgac gcaggtctgc ggcgccgatt tcacccgcgg cgtaggcgag cagcgggcgg
     1201 atgcgctttc cgccgccaag cgtggaatgg cgcatggcgg tgtgcagggg tttcggtatg
     1261 tggccggcgt cgggcagcag gcgctccagc gccagttcca cccgttcctg ccgctcgcgc
     1321 atccagccgg aaaagtcatt gctcatgcgc gttgcctggc tgggcgtagg ggtgtagcaa
     1381 cccattctca aggatttgca cttgctgttg cgcatcgttg aggacgcgct ggcagaattg
     1441 cagcagttcc gcgccgcggc gataggcatc gagcgattgt tcgagcacga gctgaccgga
     1501 ttccatttcg gccacgatgc gctccagttc ggtcatcgcg gattcgaaac tggctggcgc
     1561 aggaatgggt tggtcggtca tcttgttaca tcgggattca aaagggaaat attaccgaat
     1621 aattgcgtga aacgccaata ttggggataa tcggacaacg ctcgcggcgc ccggccagcc
     1681 cgttccggtc accatgcaat cgtggtttgc cagtggagcc gggcaaaatg cagaccggtc
     1741 ggctaagacc cgtgcgcggg cagtttccgg ttgccggaca caggggttga gcggcgccac
     1801 gccgtcttga tattcgcaag gttgtttggt cactcgtcgg ggtgtactcg tcattatgtc
     1861 tgatttggct gtcgcatcgc agttgtctcg cacgtctccc catttgccgc ttcgctggta
     1921 tttcgatcct gatatttacg cgctggaaca gaaactgctg ttcgacaacg ggccggctta
     1981 tgtgggacac gaactgatgg tgcccaagcc aggagactac caggtgctgg aaacgacaat
     2041 gcgtgcgtcg atgttgttgc gcaacgagca tggcatccag cacctgtcga acatctgtcg
     2101 ccaccgtcag gcgaccatgc tcgacggtcg cggccatgtg cgccacatcg tctgcccgtt
     2161 gcaccgctgg acatatgaca tgcagggcaa gctgatgggc gcgccgcatt ttcccgacaa
     2221 cccctgcctc gacctgccgt cgagtccggt tacgcgctgg aacggcctgc tgttctccgg
     2281 tccgcgtaac gtggtggatg acctggcggg gctgggcgtg cagcaggacc tggatttcag
     2341 cgggtatgtg ctggaccggg tcatggtgga agattatccg tttaactgga agacgttcat
     2401 cgaagtctac ctcgaagatt atcacgtcga gccattccac cccgggctgg gcaattttgt
     2461 cacctgcgat gacctgcgct gggactacgg cgaatggtgc agcgtgcagc gtgtcggcgt
     2521 ggcgcgcggc ttgagccgct tcggcagcgc ggtctaccgc gactggcagg agcggctgct
     2581 gcagtacggt aacggcgagc cgccgcgtca gggtgccatc tggctgacct attatccgaa
     2641 tgtcatggtg gaatggtatc ctcatgcgct ggtggtcagc agcatcattc cgaccggggt
     2701 ggaaagttgc cgcaatgtgg tcgagttcta ctatccggag gacatcgcgc tgttcgagcg
     2761 cgaactggtc gaagcggaac aggcggccta tgccgaaacc gccgtggaag acgacgaaat
     2821 ctgccgccgc atgcatgaag gtcgcaggct gctctaccgg cagggcaggg aagaagcggg
     2881 tccctaccag tcgccgatgg aagacggcat ggtgcatttt catgaattca tgcgcaagca
     2941 gatcggcccg catgtctgac agtcggatgc agtacgccgc agatgatatg ccgcaatggt
     3001 tattcggcgg ctttctctct gcctgatgtg caacggatcg aaggatctgc tgcgtgtccg
     3061 aaattggaga aatcatgaac cagcccgtgc agcgacgccg ttataaccgc ttgcccgtca
     3121 tccagccagc cacggcaacc agccggcgca gcggaacgct ggcaggcgaa atacgcaatt
     3181 tttccgccaa cgggctgctg ttttccgtga cgcggcctgt ggatctggaa gccttgcgcg
     3241 acgaaagcat cagcattgcc tttgtcacca gcgattcagg gccgtttcgc atctcgggac
     3301 gtgtggcgcg ggtgtttgac cgctcgctcg ggattgcagt cgacgatttt cccactgatg
     3361 cctaccgtgc gctcattttg caggccaatg ccgagcctgt gctggcgcag cagggtagtg
     3421 gcagtgaatt cggcatcacc caacgcgaag agaccctgca gcgctgcgaa cagtcttttc
     3481 atcgtttcat ggtgcagacc atcgaacggt tctatgtgcg catcaatagc cgtatcaatg
     3541 ccattcccgc cgatgcgatc gccatgcagg aacattggct gctgcgcaat ggctatacgc
     3601 aggtcagcgc gttgcggggg aaaatcgaac agtgcctggt aaccgaccat ctgactgaat
     3661 tcgaaacgct tgatgcgacg gaacagcagc aaccgcagac gctggccctg gtcgaaatgg
     3721 atacgtttga cgactggctg gcggtatccc aactggtgaa ccggttgcag caggattatg
     3781 cggtagaaat cagccgcttc gaactgcgtt atgctgcctt gaccgggata ccattgcagg
     3841 cgcgacgcaa tccatattct gcgaatgcga tgatgtggct gttccatcgt gtgctggacc
     3901 gtgccggtct gcacacccag ctcaagctgg tgctctatga gctgttccat ctcgcattgg
     3961 ccgaaggtat cgagcagttc tacgtcggca tcaacgagat tctcggtttt gttgaaactg
     4021 tgcgcgcggc gagtccggtg gcgctgtcgc cagctgattc cgcggcgcgt caggtggccc
     4081 gcgccgaggc gttgcagtcg gccgtgacgc agatgaacct gtcggctgcg ccgtctgtcg
     4141 caacagcgcc gccactggca gccgcacctg cagccgaagc tccggcttcg gtcgtgttgc
     4201 cgttcctgtc cctgcaatcg gaagcgcagc cgttcgagca ctggttgtcc ggacagcgcg
     4261 cgcaaccgca tgtggtgtcc agtgcgctgg cgagtgactc cgctgccacc ggcgccacgg
     4321 cgcctgcgat ccccgatctc gggctcaatc cgctgctgtg gcagttcaac tggatggtac
     4381 gcaacagcga gatggtctcg gggcagtccg tgctcgacat gctcgccgac tggcatggac
     4441 tgcaacgcac gagcgaacgc gaacagcgtg acgcccgcat tgcggtgtat gaatttcccg
     4501 gcgagcatgc agcagctgcc atcgccacca tgatgccgca cgcggtgcag gccggcccat
     4561 tgccgccttg cctggaaacc gcgttgggcg aaatgcgcga cctctggctg cgccgcactt
     4621 cggccacgcc ggtgcaatcg ctgcagtcgc tgctggatga ggtgccggaa ctggtgctgg
     4681 atgcggcgtg ccgtgaacgg gtgcgtccgt tgctgatgcg cttcgatgcg gcgctagggg
     4741 tagcgccgac agagacactg gcagattccg acatggccag gctgatggca aaaatgcgca
     4801 gtgcgctgat gctgcgggcg ctgcgtgatg cgtcagccct gagcgcaatg aatgatgcat
     4861 tctttcgcgg ttacgagttg ctggaacgtt ttcatgctgc tgccgacgac aacggccagt
     4921 tgttcgacaa ccggctgacg cgtctgctcg acatgctggt cgatcgtgtc gaccaggcag
     4981 tcgccggatc tgacgcggcg catggacagg aggtggcaaa tgtgctggag aacctgcttg
     5041 agagcatagg gaatgctcgc aagctgcgtg tgtcgcaggt gctgacctcc aacgcaggtg
     5101 cggtcgtgcc gacgagtgtg caacattacc cgttgttgtg tgacgaggcg atcctggcga
     5161 tggataccct ggctccgggc gactggatcg atgtcgccgg tgcgggtcgg ctggttccct
     5221 ggcaggtggt gcgtgtgggt catgatgccc agtcctggct gctggtcaac cgttcggtca
     5281 cattggtgcg cgagattgcg gctgacgtgt tcgagcgcct ctggcgcgag accgcgctgc
     5341 gcggcatccc ggaatacgcc cagcccgtac tgcaacgtac agcgatgcgt gcctggctca
     5401 atggccatgc gcgacattgg cagcttgcca tgcgtgatcc gggcagtgga ttgctcaatg
     5461 cggccggtct cgaaagtcgt ctcgactggg tgctgcagcg acgccgtcat gaagacaagc
     5521 cgttgtggct gtgctggatg cgcatgagcg gatggcaaca ggacggtgaa cagggtcgtg
     5581 cggccctgga agagctgttg cgtgacagtc tgatcctgca gtcgcggcag acgggtgcgg
     5641 tgggtctgat gggacgtcca cagacggatg tggtcgtagc gttgtgtccg gcggccgatc
     5701 cggcgcgggt gcggcgtgca ttgtctgact ggctcgatgc gcatcctggt ttcgtttcca
     5761 tgggcatccg cgtgcagcac gttgggctat gtgctgcgga tgcctggatc gaaaacgccc
     5821 ggcagtggct ggcagcggca cggctcggtg cagcggccca cctgatcgat gtggtggatt
     5881 tgccgtcggt gcgtcggcgc atggcgctgc agtcgcgtct cggtcaggtg atgcagttgc
     5941 ccgagtcggt tggctggagc ctggaagcgg cggcgtggac gcccgatgct gatgatgggc
     6001 gcaatcaccc atgtcacgaa ctgcactggc tgatcgacct cgaacgcaac ggagaatggc
     6061 gtgtggtggg cgatcggcaa gcacggccgg ccgaggccga tatgcgctgg ttgcaggggg
     6121 tgatcgaatg gctgcagggg cgcagcgagc agttgcgtca tgtcgacgga ctgggaatcg
     6181 gcctgtcgat tgattcgctt aaccacattg gattgcgcaa tctgctcgaa cgcatcagca
     6241 gcgaggactg ggcaagaaaa ctgatttttc gtctgccgga agacgcagtg agcgcgcatt
     6301 tcgaaaccgt gttgcgtttc gtgcgcgcca ctcgtggcta cggttaccgc tattgtctcg
     6361 acgaggcagg atgcctgctt ccgtcgacag cctggcttgc gcactggcgt ccggatttcg
     6421 ttcgcctgaa ccgtcagctg tcgcatggtg tgttacgcag cgatgcctgt cgcgaaatgg
     6481 cgcgcaacct gttcgatacc gcgcatgcat tggagattca cagcatagcc gaagccgata
     6541 acgatggtca tgcgacacag ttgcgtcagc tgggtgctga ctatgtgttc ggacctggcc
     6601 ggccagcgct gcgctacaat gcggaaatgc gctgagcggg atgcgtccgt aaccgcatgc
     6661 gcgtgatgga tttgcgtcag ggggccggat tcatgtgcgt cagatgaatg gcctcgatgt
     6721 cgcgcatcag ctcatcaggc agccgcaatt cccacgctga cagattttct tccagttgtg
     6781 ccagactggt cgcaccgatg atcgtgctgc cgacgaacca gcggtgatag acgaaggcaa
     6841 gtgccatgcg cgtcgctgtc agtccgtaac gctgcgccaa ctcgacgtaa gccgcgattg
     6901 ccaggggtac gccgggtttt tcgtagcgtt gtccgaatcc ggcgaaatca ttgatccggc
     6961 ctcgcgcttg cggatcggca aggtatttcc cgctcaggtg gccgaatgcc agcggtgaat
     7021 aggccagcag gctgatgcgt tcgcgccagg cgatttccgc caggctgaat tcgaaggtgc
     7081 gattgatcag gttgtaggcg ttctgtaccg aggcgatgcg cggcaggccg tgctggtcgg
     7141 ccagccggat gaactgcatg agaccccagc caaattcatt cgatacgccg acatagcgga
     7201 ttttgccttc gcggaccagt tcgtcgaggg tctcgagctg actgtggatg ctgaccgcca
     7261 gggtttcctg ggtcggatcg aactgatact ggccaaacat tggcacattg cgttccggcc
     7321 agtgcaactg atacaggtcg atatagtcgg tttgcagcct gcgcagcgaa ccttcgacgg
     7381 ccgcgcgcag gttttcccgg tcgaatgccg ccggaccgcc gcggatccat tcgagcgcgc
     7441 gacgcggccc cgcagccttg ctggcgatga tccagcgctc gcgtggctgg tgtttcagcc
     7501 agctgccgag aatgctttcc gtgcgggtcg aggtttgcgc tcgcggtggt accgggtaca
     7561 gttctgcagt gtcgataaaa ttgatgccgt ggtcggcggc aagcgccagt tggtcgtggg
     7621 cctcggcttc ggtgttctgt tgcccccagg tcatgctgcc aaggcagatg gcggagacgt
     7681 tgagcgtgct gtcacccagg tgtcgataat ccatcgatac ggttccacgt gttggacatc
     7741 gcgcaagtgt atgcgcgccg ggaccggtat gcaaccgccc ggacgcgccg ggcgggatgg
     7801 gtgttgtcta tttggtcttg caggtcctga tgccgaacgg caggtaggcc gggcaccagc
     7861 cgatggcgcc ggttacgagt ggaacgatgc cgatgtaccc ccacggcgag aatactttgg
     7921 tgacggcaag ggcaatcaga acgatgcctg cgatgatgcg aatgatgcgg tccattccgc
     7981 cgacattgca atgcataatg gtctccctat ggtgagttga gacattatta catcatattt
     8041 ttccagaaac gtgagtgacc gttgtcacac agctgccggc atcatttgca ggtgtcggcg
     8101 acgcggcgca ggttgggtgc gtcaaggatt tcgatgtgtt cgcgtcccag gcggatccag
     8161 ccgctggtct cgaactgctt gagcaggcgc gagacgattt cgcgcacgct gcccagttcg
     8221 ttggcaagct gggcatgtgt gacatgcagg tgcgagccct gtccgagcag gcgtccggcc
     8281 aggcgggcgt cgaggcggtg gaaggcaatt tcttccaccc gctgcatgag atcggtcatg
     8341 cggtcggcga acaggccaag gacgtaatcg cgaaattcgc ggctgtcgag tgctcgctgg
     8401 aatgcgtctg ccggcagcag gctgagcatg gtgtctgatt cggtgacggc gcgtgcctga
     8461 taactgtgct ggctgatcag gcaactggcg gtgacgatgc agctttctcc ggcatgaatg
     8521 cggtagagct cgatttcacg gccgttttca gcctggcgaa aaacgcgtat ccgtccttgc
     8581 agcagcagtg gaaagccctg gcagacctga ccttcggcaa atacggtgct gccggcgggc
     8641 aggtgcagcg gcgggtggct ggcgaggtgg cgagcaatgt cgggcaggcc agcgagactg
     8701 ggatacagtt gggtcaggtc agccattgcg ggtgcagcgg gcgttgagca tggtgtgttt
     8761 catgtcggct acggcctggt gccagcccgt gaatagtgcc tgtgcaacga tggcatggcc
     8821 gatgttcagt tccctgatac cgttcattgc ggcgatgggc tgtacgttgt ggtagtgcag
     8881 tccatgacct gcgttgacct gcagaccaag gtggcgagcg tgcgcgaccg cgcgacggat
     8941 gcggtcgagt tcttcctgct gcctgacccc gtgactgtca gcgtagcgtc cggtgtgcag
     9001 ttcgatgacc ggggcgccac actggtgcgc ggcatcgatc tggcgtgggt cggcgtcgat
     9061 gaacaacgac acacggattc ctgcctcggc cagttgccgg caggcgctgc cgatctgcgc
     9121 ttgctggccg gtgacatcca gcccgccttc ggtggtgagt tcttcgcgcc gttcgggtac
     9181 cagacagacg tcgtgagggc ggatgcggct ggcgatggcg agcatttcgg atgtgaccgc
     9241 catttccagg ttcatgcgcc cggtcagtgc gcgcgcgagc agttccacat cctcgtcctg
     9301 gatgtggcgg cggtcttcgc gcaggtgcag ggtaatggca tccgcacccg ccatttccgc
     9361 catcagggcg gcctgcaata cgttgggata ggcggtcctg cgtgcttggc gcagggtggc
     9421 gatgtggtcg atgttgacgc cgagctggat catagccgtt gcaggtcctt gagcaattcg
     9481 cgggcgtgca gggtttgccc gtcgagatag tgttggatga tgtgccgcat caggtgcttg
     9541 ccctgggcta cgctcagcgg atcgctgtaa tcatcgttgg ccagatcgat cagcgtcttg
     9601 ccgcgcaggg cgatcgcatc gggcggcgcc gggcgctcgc ccgccagcac agcgccactt
     9661 tcgcagcggt aatgataacc gagttcggcg tggatgggtt cgccactttc cgcttcctgg
     9721 ccaaacggtt gggcatagcc gagctgggcc agcagacgca gctcgaaccg tcgcagcgca
     9781 atgccctgtc ttgccgcgtc atccgaggcg cacagttcgc gcagcgtgtc ctgatagggc
     9841 tggaacaatc ccggatgcgg gtcttcgcgt gcaagcagct tcagcagcag ttcgttgaca
     9901 tagaagccgc acagcagcgc catgccttgc ggcagcggga gcccaccctg ccactgggcc
     9961 gcgtgcagcg tgcgcaattc ctgcctgccg gaccagctca gcagcagtgg cgtgaacggt
    10021 tgcagcaggc cacgcagcgc ggagcgcggc cggcgggcgc cacgggccac cagtgcgacg
    10081 cggccatagg cttcggtcca ggtttccacg atcagactgg tttcacggaa cggacggctg
    10141 tgcaggacaa atccgctggc atcgtccact ctggcagcat cggtacgcgg cggcatggcg
    10201 gcggaggcgg ccctcagtcg tagcccagct tgcgcagcat ctgctcgctg tctgcccagc
    10261 cgctgcgtac tttgacccac agttcgagat agaccttgcc accgaacagc tgctccatgt
    10321 cgatgcgtgc tgcggtgccg atctcacgta atttttcgcc attgcgaccg atcaggatcg
    10381 atttgtgatt gtcgcggtct accaggatcg aggcaaaaat gcgtcgcagt gcaccttcct
    10441 gttcaaactt gtcgattacc acgctggtgg cgtaggggat ttcttcaccc gagaggcgga
    10501 acactttttc gcgcacgatt tctgcggcga gaaaacgttc gctcttgtca gtgatgtcgt
    10561 ctgcgtcgaa catcggcggt tgcgctggca gcagggggcg gatcgtctgc agcagcgttt
    10621 cggtgccgtc accatgacgc gcagacaggg gaaccatggc agcaaaagga aatttgtcgc
    10681 gcatgctggc catgaatggc agtaacaggt ctttctgctt gagcaggtcg agcttgttga
    10741 tgacgagaat gaccggaaca tcgcgcggca gcagtggcaa caggctttcg tcgcgccggt
    10801 cgaagtgcag cccttcgacc acaaacagca cgacatcggt cgagcgcagc gcttcggtga
    10861 ccgatttgtt catgttgcgg ttgagcgcgt tgcgatactg ggtctggaaa ccgggagtgt
    10921 cgaccaggat gtactgcgca tcatcggtgg tgtgaatccc gtggatgcgg tgccgcgtgg
    10981 tttgtgcctt gcgcgaggtg atgctgatct tggcgccgac gatgcggttg gtcagcgttg
    11041 attttccggt attcggccgg ccgacgatgg cgatgaaacc ggtgcggaac acctgggctg
    11101 cgccgtcggc gggggtaagt tcgctggtgg tgttcatggg gcgcgccgcc gagtcgtgcc
    11161 acgcgatggc tgttgctgga tgagctgcca cgcggcatct gcggctgcct gttcggcagc
    11221 gcggcggctg ctggcactgc cccgggtgac gatggacagg gccgggatgc tgcactcgac
    11281 atgaaaggtc tgctcatgcg cctggccgct ggtgctgccc agcgtgtagt cgggcagggg
    11341 caaacgctcg ccctgcaggt attcctgcag acgggtcttg gcgtccttgg ccggctgttc
    11401 cgggttgatg gcatcgatac tgtcggcata cagccgtgcg acggtctggc gggcagcgtc
    11461 gaaaccgccg tcgaggaaaa tggcgccgag cagcgattca cacgcgtcgg cgagaatcga
    11521 cgggcgggtg cgtccaccgc ttttctgttc tccgtcaccc agttgcaggt atttgcccaa
    11581 ctgcagcatt acggcaatgt cggcgagact ctgttgacac accaggttgg cacgcagacg
    11641 cgagagcgcc ccttcagtga gtgtgggaaa ggcgtcatac agcagcagcg ctacggcgca
    11701 attgagcagg ctgtcgccga gaaattcgag acgttcattg tgctgcgcgc tgaaactgcg
    11761 gtgagtcaac gcctggcgca gcagttcgcg gcgggaaaac ctgtagccga tggcctgttc
    11821 aagcgcatct gcgttcattg ggttgccggg ctggtggtgg gcgcgaaatc gatgtagatg
    11881 cctacgttgc cgaaaatggg tttttccacg tgataactcg cgctcaggtt gaggcggttg
    11941 tcaccttgat cgattttgag atcagaccca ttgatgctgg tgatgttgtc gatctgcgag
    12001 cggcgattga atgagtcgcg gattgctgac ggaggcgcgc cggtcagtgc aggatcggtc
    12061 accatggcac tgaagacacg ctcgatcgac aggtattcca tgtaggaggg caatacccgt
    12121 acgaccatga cgacgatgaa aaaa
//
LOCUS       DAIANL010000110         2690 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1770349, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000110 DAIANL010000000
VERSION     DAIANL010000110.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770349"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          610 a          835 c          743 g          502 t
ORIGIN      
        1 tctgcttata ggcgctaccg ggaaacaacc cgttgtccgg attttgacga tattccgcac
       61 cgaccagcca ttgattggca gcatccagaa atacgccaac cgatccttca aactcggttg
      121 aataattgtt tttggtcggg ctggatattc cggcataacg tgaccaccga ttctggttta
      181 tcgtgaccgg tgattccggc gcaacatgac cgctgattct ggtttatcgt gaccgatttt
      241 ggggtgtttc cggaatcggt ggtcacgata ccggaatgac tggtcacgat ggcggaatgc
      301 tgtcgtatgc catgaaactg gtgttacgca tcgtgcaacc gaacagggta cgcttccggc
      361 tttttggtcg gagacagcat gcccgtagca aggatcacca tgcgtaagat taaagagata
      421 ttgcgtttaa aactggacgc gcggctgtct caccagcaga ttgccgcagc ccttggcctg
      481 tccaagggtg tcgtcaccaa atatatcggc ctgacagccg cggcaggtct ggactgggct
      541 gccgtgcagg atatggacga agccatgctg gaacgacggt tgttggtcgg cccggaacgg
      601 ccacgcaatc atgtccaacc tgattatggt cgtctgcacc aggaattgcg ccgcaaaggc
      661 atgaccttga tgttgctgtg ggaggaatac cgcgcagacc atgccgaccg gcaaacgtat
      721 gcctattcgc agttttgcga gaactaccgg cgcttcaccc ggcaactcaa gcggtcgatg
      781 cgtcaggttc accgcgccgg cgagaagctg ttcattgatt tcgccggtcc caccattgcg
      841 ctgaccgacg gcagtcgtgc gcacatcttc gtggcggcgc tgggtgcgtc cagctatacc
      901 ttcgcctacg ccactccgcg cgaaaccacg gctgactggc tgcaatcgac agcatgcgcg
      961 ttgcgtttcc ttggcggtgt gccgcagatg atcgtgccgg acaacccgaa ggcgctgatc
     1021 gctgacgcca accgctacga accgcgcaat aatgacaccg tgctcgactt cgcccgccat
     1081 tatgggacaa ccgtattacc ggcccgcccc cggcacccgc aggacaaagc caaggcagaa
     1141 tcggcagtac aggtcgtcga acgctggatc atggcacggt tgcgtcaccg gcaatttgcc
     1201 agtgtgcatg aagtcaatca ggccattgcg ccattgttga aacaactcaa cgacaagccg
     1261 ttccagaaac tgcccggcag tcgtgtcagc gccttcgctg aaatcgatgc acccgcgctg
     1321 gctccgttgc cgttgcagcc ctatgaaatg gcacacttca aaaccgtcaa ggtacatatc
     1381 gattaccaca tagaaatcga acgccaccat tacagcgtac cgcatgcgct ggtcggtcag
     1441 atcctcgatg cacgcatcac cgccaccaca atcgagctcc tgtatcgtgg caaccgtgtg
     1501 gccagccatg cccgcagcag cctggctggc ggctatacca ccaccgcagc ccacatgccg
     1561 gcggcgcacc gcgcgcagat ggaatggtcg ccacagcgtc tgattcactg gggggaaagc
     1621 atcggacctg caacggccga agtggtaacc cgcctgttga gccagtacaa acatcccgag
     1681 catggttacc gcgcctgcct gggtctgctc tctctcgcaa aacggtatgg tcaatccaga
     1741 ctcgaagccg cctgcatact ggcgttacaa atcggagcct gccaataccg ccatgtgcgc
     1801 gacatcctga agaacaaccg cgatgcaagc attccgccgg aaaccaagga ttggatcagc
     1861 cccgaacatg cgcatgtgcg cggctcaggc tactaccaat gaggaacgat caaatgatga
     1921 tgcataccac gctgacgcaa ttgcgcagcc tgaaactgga tggcctggca acaggcttgg
     1981 aagagcaact ggcgcaaccc gcgatgtccg cactcagctt tgaagagcgc atagccctgc
     2041 tggtggatcg ggaagtgcat gcccgcaacg accgtaaact ggcgcgcctg ctaaaaaatg
     2101 caaaactgaa atacggtcag gcgaccattg aagacataga cagccgaaca gggcgcggca
     2161 tcgaccggcg cgaggtgatg agcctggctc tgggcgactg ggtcgatacc ggtcacagca
     2221 tcctgattac cggccccacg ggtgccggca aatcctggct ggcttgcgcg ctggcgcaat
     2281 acgcctgccg ccggggttgc tccgccattt accagcgcgt accccgcttg caagaagaac
     2341 tgcgcatccg ccacggcagc ggcaccttcg gcaaatggct tctgcaactg gccaagaccg
     2401 acgtactggt actcgacgac tggggcatgg gcgccatcga cagcatgacc cgctccgacc
     2461 tgctggaaat catcgacgac cgcgccgcca gcaaagccac cctcatcacc agccagttac
     2521 cggtcgaaca ctggcatgcg tggataggcg acgccaccat cgccgacgcc atcctcgacc
     2581 gcatcatgca gcgcaaccac cggtttaccc tcaccggcga ttcactgcgc gcagcgccat
     2641 cccaaacccg caaaaaggag gaaaacctga catcatcgtg accgcaagca
//
LOCUS       DAIANL010000111         7692 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3205242, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000111 DAIANL010000000
VERSION     DAIANL010000111.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 7692)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7692)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7692
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3205242"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1140 a         2086 c         2730 g         1736 t
ORIGIN      
        1 gtgtctgcat gaaaatagcg gcgagatctg ccttcaccca gcgcagcgac ctggcctttc
       61 tcgatcagct ttgcgatcaa tcgttgcgct gttctctggc aatgccgggg tgttcggcca
      121 ggagttcggc cagcgtcaat ccgttcccgg atgtccggat actggtcagt aagtctgtgg
      181 tggatgacat agcggatttg gcgctgtttg aagtgggtgt ggcgcaattt gtcaaactgc
      241 gccagtcgca ggcctgtagc tggcgcagtt aatgtcaact gcgccatatg gaagcaacag
      301 gggatgtctt ccgtgcgagg ctatcctgcg ctattgccag acttgacgat tattctgcac
      361 gcttccatca tgacggaatt gcatctgccg atgtgttcga gaaataagcc atgaccgttg
      421 agtcgtttcc ggaatttacc cggccagccc gtcaacgatg ggattccatt cccgccgaac
      481 ttcgtcagcg tatattatcc aacgtctggt gcgggcattg ccgcacttcg gtgacgatca
      541 cgcatttcag gggaagtatc cggggtgggg atctgctgct ggagggggaa tgtgctgcgt
      601 gccacaacgc cgtggcgcgg gtgatcgagc gctcgtagtc ccgcatcacc cgccgcatcc
      661 tgccatcagc ccagcagatg gtggtattgt tttttggtac tgttgacttt ttgcagcgca
      721 ttctttgcgg cgttcaggtc gtttgcgcgc acggcctggt tgacggcgtc catctcggtt
      781 tgcatttgtt gcagcccttt gtgataaacg gccggattgc ggcggtactg cagcttgtcg
      841 gccgtgatgg catcgttctg caagcgggtc gagtactggg taaactcggc aatcgaggtg
      901 ctgttcatcg cgcctctcat ggcggctttc atgtccttca tgacgttttt catttcgctc
      961 gcatggacct gagcgctgga cagcagcgtt gccagaagca gcagcccgcc aattttacgt
     1021 attggatgag tggtgttttt cattgcgctc gctttcatgt gtgacgtggc agataaggaa
     1081 ttctaccgtc cgatgcttag gccagaatga gggctttgaa aaccgttggt tcgccgatgt
     1141 tgtacatcgt gcggcggcgg gttgttccgg gaaaatgttt cgccgcaccg ggggacgtcc
     1201 tgttggggtt tgcctcgggc gcataagcgt gtaaattggt ggctgatgtc gagcggcgat
     1261 gtgtgtcgcc tcatgtggcg cagatgtgcg ctcctccgta cagcctggat cgttttccgt
     1321 ttcatgtcta ccctgattga tttctgcgcg gcggctgccg ctgtcggtct ggccttgttg
     1381 accgcgcaac tgcttgcatg gcgttttggc ccgcccgcgc cgatggggcg ctatctcgcc
     1441 atcgatggcc tgcgcgggtt gctggcgttt tctgtcatgc tgcatcactc ggtcatctgg
     1501 tatggctatg cgcacaccgg gctatgggtt gcgccggttt cgcatgtatt tgccgaactg
     1561 ggcgagggca gcgtgctgct gtttttcatg atcaccagtt tcctgttcat gggcaagatc
     1621 agcgatacgc gcgcgccggt ggactggctg cggctgtatg tgtcgcgtat cttgcgactg
     1681 acgccgctgt actggctggc gatgctgggg ctgatgctca tggtgggctg gctgtccggc
     1741 tggacggtac gtgtgccgct ggcgcagttg cgcaaccagg tgcaggtgtg gctggggtat
     1801 ggtgtgtcgg gttatccgga tgtgaatggg gtcgtggata cctggcatat cgtggcaggg
     1861 gtcacctgga cgctggtata cgaatggctg ttttatatgg cgctgccact gctggcattg
     1921 ttgatcggac gcaggccggg gtgggctgcc gtggtgatga gcgtgctggt cttgtggtgg
     1981 atgttgacgc agtggcatcc caatccgcgc atgctggtgg tctttgcgct cggcggaatt
     2041 acggcactgt atgcgctgcg ccagcaattt ctgctgcgtt ttgccacgac atcgctggcg
     2101 ggttgggttg cgctgggctg cttgctgctg gtgctgacgc tgttccccac ggcctacgat
     2161 gcctggccaa cgctgctgct gtggggcgtg ttcgtgctgg tggtctgcgg caatacgctg
     2221 ttcgggctgc tggtgcatcc ggcgacgcgc atgctcggcg aaatggccta cagcgtgtac
     2281 ctgttgcatg ggctggtgct ctacctgctg tttcatgtgt ttctgggaga ggcggcggtg
     2341 cgcagctgga caccgctgca tttctggctg gtgttgctgg ccatcgtgcc cggggttgtg
     2401 gtgctgagtt tcatgacctg gaactgggtg gagcggccgg gcatgcgtgc ggtcgcgccg
     2461 ttgatggggc ggctgcgtca ttcttccttg ccttgaacgg cggggctgtt gcgtgccggg
     2521 tcagtagccg ctggccaggg ggtcagcggc aggtttccag ccatgcgggc gggcagcggc
     2581 gggtccacgt tgccgggcct ttggcgggca attccagttt gtggcgggtg taataggtgc
     2641 ggtacgaagc cactacatcg tctttcaggc agtcggcata acgttgctgg tcgaaagcca
     2701 cgacgaaggg cgtgcgtgtc tgttgcggaa aagggatgtc gcacgccagc acatattgct
     2761 gcagccgcgt cagcatgctt tcggctccgt gtcgtttgtc aaaacggaag gcccattccc
     2821 gacacagtga cagccccagt tgtgcggccc agagaaagtt gtcgcgcgaa gctgccgccc
     2881 attgtgtggc ggggtggaac gggtgggtga cgccccagcc ggcttccagt ggttttttcc
     2941 agcgtttttt ggtcatgccc gggcgggcgg cgggaatttc gtcgtagctg tgcagcacga
     3001 ccgccagcgc ggcggtgcag atctgccccg cttcggttgc cattttgttg cagtgcacat
     3061 catggtgcgc catggccgca agttgcggat cggtatccag gatgaaaaga ttcatgcggt
     3121 ggccaggttt cgtctgacgt tatccaggtg ggtttttttc agctgcggcc atgcgtgcga
     3181 gcgtgtcatg caggccggtc gcagggtgcc agccgagttc gcggcgaatc ttgctgctgt
     3241 cgatttccag cgagccgagc agccggtcga gtgcggcacc ctgaccggtc aggcggccca
     3301 gtgcatgcag ccagcggggc ggaaacggca gcagacgggt gcgccgaccc tgcgctgcgg
     3361 cgatggcgcg cagcagttgt gcggtcgaga ccgctgcgcc atcgctcagc agataggtct
     3421 ggttggcggc agcggggtgg ctggcgcagg ccagcaacgc gctggccagg ttatccacgg
     3481 ccagcaggtc gcgccggttg cgcacggaag ccagcggcag cggccagctt gccatgtgca
     3541 gcatgcgggc aaagttgcct ttcacgcccg ggccgtacac cagcggtggc cgcacgatca
     3601 cgatttccag cccggtgtcc cgcccgatct gccacagcag ttgctctgcc tcgtgtttgg
     3661 agcgggcata agcgtcttgc gggtgcggtg cgtcggtttc gcggaacggc tgtccgggca
     3721 gcgtggcttc gccattgacc ttgatgctgc tgacatacac cagccgttgt acgtcggcag
     3781 ccgctgcggc acgagccaga tgcgccgtgg cggtgacgtt ggcccggcgg aattcggcca
     3841 gcggatcggc ggccgattcg tgcatgacat gaacccgcgc tgccagatgg atcaccgtgt
     3901 tgcaaccgcg cagcgcagct gtccagtcgg tgtcggcatc gatattgccg acgacggcat
     3961 tgcgcgtgcc agggcagggc caggcggatc gggtggcggt ggtgagcgga aaccccgatt
     4021 gcagcgcttg ccggaccagc gcctgaccga cgaatccgtt ggcgccggtg acgagcaggg
     4081 caggcgggtg gatgatggtg ggcatgggcg ggttcgcgat ggggtgatca acggaggggc
     4141 cattctagcg cacggcgggg cgaacgtgcg cgtgttgccg gccgcaggcg cgtatgggcg
     4201 gttgcgatga cgggcgacct gccgcgcatg aattgatgaa atttgtgatg taatgatgca
     4261 tcattgccgg gcggggccga tcggtatcat gcgggtcatg tttcggtgcg ccgtgtcctt
     4321 gcgggccaca gcgatggcac catcgcacaa cgcgacggca tgggtgccgg gcaggcgagc
     4381 cgatgctggg cgaaatcccg cgtcgtgggg caggcgtggc ggtgctggcg gcgcagcatg
     4441 tggcgaatgt cgaagatgtt gtgccgttgc agcagcgtct gctgcgaaag agcgaggcga
     4501 tggaccggga agtgatatta cgcaaggtgg atgctttgca gcgttgtctg cagcgcgtcg
     4561 agagtcgttg tctgcacagt ggtgacggtc tggcacagca tccggatgcc caggacatcg
     4621 taccgttgaa tctgatgcgg gcggtgcaat tgcgcgtgga catcgccctg cagtggccgg
     4681 cggacgcgcc gcaggctgaa ctgcctgaac tgccaacgac gatggggggt gccttcgagg
     4741 cattggcgca gcagggcagt atcaccccgg cgttggcgtt acacatgaag caggcggtcg
     4801 ggttgcgcaa tctggccatt cacgcttatg aaacgatcga ttgggagatt gtgcatgcaa
     4861 tttgcagcag ccggttgggg gagtttcgcg tgtttgccgg cgcggcgccc gaacagcctg
     4921 cctgacggat cgggtgtggc gaggacatgc tgatgagcgg gttgtggcat tttgctccgg
     4981 tttttgcggg gttggtcagt gttgttgcgg tgtggttgct gtccggtccg ctggccggac
     5041 tggcgatgga tcatcccaat cagcgttcat tgcaccagcg tccgatcccg cgcaccggcg
     5101 gactggggct gatgctgggg gtcgtcgtcg gctggtggct gtccggggtg cgcctgccgc
     5161 tgtctctctg ggcgggtgcg ctactgctgg tgggggtgtc gtttgccgat gaccggctcg
     5221 atctgcctgc cgcgttgcgt ttcggtctgc atctggtggt ggcgggctgg ctggccgggt
     5281 cgctcgggct gaacggggtg tggctggtgc tggcactgct gtcgacgggc tggatgacca
     5341 atctgtacaa cttcatggat ggcgcagacg ggctggccgg tggcatggcg gtcattggtt
     5401 tcagtgcgct tgcggcggca ttccagttgc agggcgaatc gggctgggcg ctggctgcgg
     5461 tcacggtggc agtggcagcg ggaggtttcc tgctgttcaa ctggccgcct gcgcgtgtat
     5521 tcatgggcga tgccggctcg gtcccgctgg gttttctggc ggcggcgttc ggctggctcg
     5581 gatttgcgca gcatctgtgg ccggtgtggc tgccgttgct gctgtttctg ccgttcatcg
     5641 ccgatgcgag cgtgaccttg ctgcggcgcg ggttgcgtgg cgagaaagtg tggcaggcgc
     5701 atcgtgaaca ttattatcag cggttgatcc gcatgggctg gagccaccgg cgcatggcgc
     5761 tcgtggcgtg gacgctgatg gcgtgcggtg cggcgctggc gctgcttgcc tggcgcgtgc
     5821 cggcctggcg ggtgctggca ttcgcctggc cgttgtgtct ggcggcgggc atggtctgga
     5881 ccgaccggcg ctggcgggca tggcaggcgc aggcggacag cgcaacccgt tgaaaccggc
     5941 tggctgaggc catgcgccac agacgtgatt tgcctcttgc cgcctggggc cgggcgcgtt
     6001 aaccttcggc agttatctgc aaccggagtc agccgtggac cgttaccacc ctcctgtatt
     6061 tccgcgcagc ccgcggctgg acgggccaga tgccgaaacc gtgcgccaga acgtgcgtgc
     6121 gtatttccac gagacgtttt cgcgctacga acagttgttc gaactgctgg cctgtgacga
     6181 agcgtactac cacaaaccga ttcgcctgcg gcatccgctg attttctatt ttggtcatac
     6241 cgcgacattc ttcgtcaaca agctgctgat cgccggtttg ctggagcagc gcatcgaccc
     6301 ggggttcgaa tcgatgtttg ccgtcggcgt ggacgagatg agctgggacg atctcgatga
     6361 ggcgcattac gactggcctg cggtggcggc ggtgcgagcc tatcgcgacc agttgcgcga
     6421 ggcggtcgat gcgatcattg ccaggacgcc gctgcagttg ccgatcgact ggaaccaccg
     6481 ctggtgggcg atcctgatgg gtatcgaaca tgagcggatc catctggaga cttcttcggt
     6541 gctgattcgc cagcagcggc tggaatgggt tcgaccgcac ccagcctggc cggcctgccg
     6601 gctcacgggc gagttgccgc gcgagtcgcg ggtggagatt ccgcccggta cggtgcgcat
     6661 cggcaaggcg cgtgacgatg tctggtacgg ctgggacaac gaatacggcg aacacgaggc
     6721 gcacctgcca gccttccagg cagcgcggca tctcaccagc aatgcgcagt tccttgcgtt
     6781 tgtcgaggcg gatggctacg ggcaggatgc gtggtgggac gaagaagggt tgtcatggcg
     6841 tcagtttgcc cacgccagac atccggaatt ctggcgccac gagcagggac gctggcggct
     6901 gcgtctgctg gccgaggaag tcgacatgcc gtgggactgg ccggtggagg tcaattgcca
     6961 tgaggcgcgt gcctggtgtc gctggcgcgc cgcacagggc ggcgaactgg ttcgcctgcc
     7021 gaccgaggac gagtggtacc gcctgctcga tgtcgccggg gtgggcgaac tggcggccga
     7081 tgcgcctgct gcggccaatg tgcatctcga tcactgggcg tcgtcgtgtc cggtaacatt
     7141 gtttgcccag ggggaactgt tcgacattat cggcaatgtc tggcaatgga ccgaaacgcc
     7201 catctatccg ttcgacggtt ttgcggtgca tccgtattac gacgatttca ctgtgccgac
     7261 gtttgatggc cggcataatc tcatcaaggg cggttcgtgg gtgtcgtgtg gcaacgaggc
     7321 gcgtcatgca gcgcgttatg cctttcgccg tcatttcttc cagcatgccg gttttcgtta
     7381 cgtggtgggc gcaatgccgg cgcgggcacc cgacccgtat tacgaaaccg atcgccagtt
     7441 gtcggaatat gccgaatttc attatggcga tgaatatttc ggcgtggcga acttcccgcg
     7501 cgaaatggcg cgcatcgccg tgcaggccat gcagggtcga cccatgcgtc gcgcgctcga
     7561 tctgggctgt gcggtgggcc gcgccagttt cgagctggcg cgagcattcg aacgggtggt
     7621 cggcgtggat ttctctgcac gtttcattca gatgggggcg gcgctggcgc acagcggcgt
     7681 gttgcgctac ag
//
LOCUS       DAIANL010000112        15225 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2176440, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000112 DAIANL010000000
VERSION     DAIANL010000112.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 15225)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15225)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15225
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2176440"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3038 a         4491 c         4921 g         2775 t
ORIGIN      
        1 ttgcgctggc cgcccggatc accgctgacg ccgacatgca gcggcttgcc agtgatcccg
       61 ccttcggcag ggaacgctat gccgcgctgc tgctcgacgt atgcggcaag aatgtcaccg
      121 ggcccgttgc caactttttg catctgctgg tcgagaacgg ccgcattggt gtgctggccg
      181 aggttgtggc ccagtttgag acgctcaagg cacaggccag cggcatcaag gacgcaaacg
      241 tggtgagcgc atggccactg gacgagactc aggtcaaggc gctggcagaa cgcctgcgca
      301 aacgcttcgg ctgcacggtc aacctgtcgg ttcaggtcga tcccgcactg atcggcggcg
      361 tgatcgtcac cgtgggtgac gaagtctacg acgcgtctgt ccgcggcaaa ctgcaggaca
      421 tggcctacgc gctgaaacgt taagaatcta atcaggagca atcaatgcaa ctcaatccta
      481 ccgagatcag cgatctcatc aaaagcagga tccagaatct gggcgcggct gccgagttgc
      541 gcacgcaagg caccgttgta tcggtgaccg atggtatcgt gcgcgtccat ggcctgtcgg
      601 atgtcatgca gggcgaaatg ctggagtttc ccggcaacac cttcggtatg gcgctgaacc
      661 tggagcgcga ctcggtgggc gccgtggtgc tgggtgaata cgagcacatc acggaaggcg
      721 acatcgtcaa atgcaccggg cgcattctcg aagtgccggt cggcgaagcg ctgattggcc
      781 gtgttgtcga ttcgctgggc cgtcccatcg acggcaaggg cgcgattgcc tccagcggca
      841 gcgctccgat cgaaaagatc gcacccggcg ttattgcgcg ccagtcggtg aaccagccgt
      901 tgcagaccgg tctcaagtcg atcgactcga tggtgccaat cggtcgcggc cagcgcgaac
      961 tcatcatcgg agaccgccag accggcaaga cggccgtggc cgtggatgcg atcatcaacc
     1021 agaaaggtac cggcgtcatc tgtatctatg tggctgtcgg acagaaggcg tcgtcgatcg
     1081 ccaacgtggt gcgcaagctc gaagagcatg gcgcactggc gcacaccatc gtcgttgccg
     1141 caactgcatc cgactcggca gccatgcagt acatcgcacc gtactcgggc tgcacgatgg
     1201 gcgaatactt ccgtgatcgc ggccaggacg cgctgatcgt gtatgacgac ctgaccaagc
     1261 aggcctgggc ctatcgccag atctcgctgc tgctgcgccg cccgcccggc cgcgaagcct
     1321 accccggcga cgtgttttac ctgcactcgc gcctgctgga gcgtgccgcg cgcatcaatg
     1381 ccgagcacgt ggaaaagatc accaatggcg aagtcaaggg caagaccggt tcgctgaccg
     1441 cgctgcccgt gattgaaacc caggctggcg acgtgtccgc attcgtgccg accaacgtga
     1501 tctcgattac tgacggccag attttcctgg aaaccgatct gttcaatgcc ggtatccgcc
     1561 cggccatcaa tgccggtctg tcggtgtccc gcgtcggtgg cgccgctcag accaaggtca
     1621 tcaagaagct cggcggcggt attcgtctcg cgctggcaca gtaccgtgaa ctggcggcat
     1681 ttgcgcagtt tgcctccgat ctcgatgaag ccacccgcaa gcagctcgag cgcggcaaga
     1741 tggtgaccga actgatgaag cagaaccagt atgcgccgat gaacatcgcg gatatggcgc
     1801 tgaccctgtt cgccgtcaac aagggctaca tggacgacgt ggaaatcaag aaagcgctgg
     1861 cgttcgaggc ggcatttgct tccttcatga agtcacgtca tccggcggtg ctcgatacca
     1921 tcctgcagac gggcgacctg tccggggata atgaagcggc gctgtccaag gctgtggaag
     1981 aattcaagtc cacctccgcc tactgataca ggactgaatc atggcttccg gcaaagagat
     2041 ccgaaccaag atcaaaagcg tggaaaatac gcgcaagatc acccgtgcca tggaaatggt
     2101 tgcggccgcg aagatgcgca aggcgcagga tcgcatgcgc gcagcccggc catacgcaga
     2161 gaaaattgct gccgtggctg cccatctgtc gtttgcaagc ccggagtacc ggcacccgtt
     2221 cgtgcagaag cgtgaacaga tccgccgtgt gggcctgatt gtggtctcga ccgacaaggg
     2281 cctgtgcggt ggtctcaaca ccaatgcctt gcggcttgcg gtgaatcaga tgaagaagtg
     2341 ggaagccgaa aacatcggta tcgacgtcac ggcctttggc aacaaagggt tggggttcat
     2401 gcagcgtatg ggcgccaatg tggtatcgca tctcgtggct ctgggcgaca ccccgcacgt
     2461 cgaccgcatg atcggcccgg tcaaggtgat gctcgatgcc tatgtggaag gcaagatcga
     2521 cgcgctgtat ctggtctaca gccgcttcat caacaccatg aagcaggaac cggcgctgac
     2581 gcagttgctg ccgcttgccg gtgagcccga tcagcagctc gaacaccact gggactacat
     2641 ctacgaaccc gaggcacgct cggttgttga cggattgctg gtgcgttacg tcgaggcact
     2701 gatctatcag gccgtggccg agaacatggc ctgcgaacaa agtgcgcgca tggtggcgat
     2761 gaaggcggcc tccgacaacg ccaagaatgt gattggcgag ctcaagctgg tgtacaacaa
     2821 aacccgtcag gcagccatta ccaaggaact gtcggaaatt gtggccggcg ctgcggccgt
     2881 ataagctatt ttgaacattc aaggaacgac gatgagtcaa ggcaagatcg tacaaatcat
     2941 tggcccggtg gtcgatgtgg aatttccgcg cgacagcatg ccgaaagtat atgacgcgct
     3001 caagatggag tcgcccgaac tgacgctgga agtccagcaa cagctgggtg atggcgtcgt
     3061 gcgtgcaatc gcaatgggta ccacagacgg attgcgccgc ggtctcggcg ttaccgcgac
     3121 cggcgcgccg atctcggtgc cggttggcgc caagacgctg ggtcgtatca tggatgtgct
     3181 gggcaacccc atcgacgaaa tgggtgagat cggcaatgag tccacctggg cgattcaccg
     3241 caaggctccg gcgtttgatg aactggccgc atccaatgaa ctgctggaaa ccggcatcaa
     3301 ggtgatcgac ctgatctgcc cgtttgccaa gggcggcaag gttggcctgt tcggtggcgc
     3361 aggcgtgggc aagacggtga acatgatgga gctgatccgc aacatcgccg tcgaacacag
     3421 cggttactcg gtgttcgctg gcgtcggcga gcgtacccgt gaaggcaacg acttctatca
     3481 tgaaatgaag gaaggcggcg tgctggacaa ggtggccctg gtgtacggcc agatgaacga
     3541 gccgcccggc aaccgtctgc gtgtggcgtt gactggcctg accatggcgg aatatttccg
     3601 tgacgaaggc cgtgacgtgc tgctgttcgt cgacaacatc taccgctata cgctggccgg
     3661 taccgaagta tccgcactgc tgggccgcat gccatctgca gtgggctacc agcccacgct
     3721 ggctgaagaa atgggccgcc tgcaagaacg catcacttcg accaagaagg gttcgatcac
     3781 ctcgatccag gccgtctatg tgcctgcgga cgacctgacc gacccgtcgc cggcgaccac
     3841 gtttgcccac ctggacgcca ccgtggtgct gtcgcgccag gtggccgaac tggggatcta
     3901 ccccgccgtg gatccgctgg actcgacatc gcgccagctc gacccgctgg ttgtgggcga
     3961 agagcactac ggcgtagccc gtggcgtgca gtcggtgctg caacgttaca aggaactgcg
     4021 cgacatcatc gcgattctgg gtatggacga actggcaccg gaagacaagc tggtcgtggc
     4081 ccgtgcacgc aagatccagc gttttctgtc gcaaccgttc ttcgttgccg aagtctttac
     4141 tggcgcaccc ggcaagtacg tatcgctcaa ggacaccatc agtggcttca aggcgatcgt
     4201 caatggtgaa tacgaccatt tgcccgaaca ggcattctac atggtcggca cgattgaaga
     4261 ggcagttgag aaggccaaga ccatccagta atggctgagg cagtaatggt ttgccgggcg
     4321 gtcaggtcat caggtcctga ctgcccggca agacgacatt gccgtccccg aagcgatgct
     4381 gcggagcggc cagaatgagc gggaacacag agttatgtcc atgacaatac acgtcgatat
     4441 cgtcagcgca gaaagttcgc tgttctccgg cactgcggaa tacgtgatcg taccggctga
     4501 aatgggcgaa atcggggttc atccccgcca tacccagctg ctgtcgcgac tcaaaccggg
     4561 ttcggtccgc atcaagaaac cggacagcaa tgaagaagaa ctgatctatg tgtccggtgg
     4621 catggtggaa gtgcaaccgt tcgtcgtgac gatactgtcc gatacggcca ttcgcggtca
     4681 ggatctcgat gaagagcagg cgctcgaagc gaagcgtcgc gctgaggaag cgatgaaact
     4741 gcgaacctct gaaatggatt acgccaaagc cgaagccgaa ctggcagatg ctgtggcgca
     4801 gatcgccgcc attcgccgcc tgcgcaagca gcactgaacg gttgccaccc gaaggcgaat
     4861 gccttcagcg tgtgcgtgtg gtgccgcaca gccactccgg gcccgacgca tgttctgcgc
     4921 tcgggcccgt gtgtttcagc cacccggctg acgtgacggg taggccagta acagcttgta
     4981 attgctcacg ttgagcgtgc cgatgtccag caccagttcc gccgccatat cgctgccggg
     5041 ggcaattccc cgagcggcag cctgagtgtc ggggagatag tcacccggag cgaaattttt
     5101 ccgcaccagc agttgatctc catccgagct gaggctcagg ctgatgtcgg gaaactgctg
     5161 agccacctcc gaagtgtttg ccatgctcca atgcacatgc agtcggtgcg gatcaaccgg
     5221 atcgacagac agttcggtgc tggtcagccg tagcgcatcg aggtcgcgtg gcagccgagc
     5281 accacagtcc ggctctccgg cgcacagcac aaacagtgcg ggtttcagtg ccggtacccg
     5341 tacggcaatc agatcgcgct cgtgccagac cacttgcccc gcaagcagga caagcaacag
     5401 cagggacagc acgagcaggc cccacggtgt gccgctgcga cgggcggttg caaatggcga
     5461 caccgtcgca ttgtcggcag gcaccgcgac gacagccgct gccgcaatat cgccgtctgc
     5521 agcgtgggct gcagcggtgg gcacagccgg caggggatcc gctgcaaccg cttctgtcac
     5581 ctgatccgca gcggcgacgg tgaccggcgg ggtcgcagtc tgcacgtcat tatccgttgc
     5641 gccgacggtc tcgcggatgg ccggggggtc atacagcgca gataacggtg ggatttccat
     5701 ccagtcgaga tcggcatctt cagcgggcga aacggtagcg tgtgctgcat gctggtcagg
     5761 gtgtggcgca atggtcgcaa aagtcgtgtc cgccgttggc gctgaatcag cagactctac
     5821 gctgggtacg gcagcaggca atggctgcgt ctgcgagtgc tcgacattgt tgctggtgtg
     5881 ctggtctggc ccggtgtcgc tgctggtggc cgctaccggt gcggatgccg gcgcatgcag
     5941 atcggaagca aacgaatcga tatccgggat gtgcgcatca agcgcgttga acggctggcc
     6001 gcagacacca catcgcgccc gcccggccgt agcgacaagc tggcgggcat cgacgcgaaa
     6061 cacactgtga cattgtgggc accgggtact aaagctcatg gtttgcgcat gccgctcagg
     6121 cagatccagc cttcatcttc agcaaaggtg tgcagatcat accacggggc atacgccaag
     6181 cggatatcct ccgcctgtga tgacagaata ccggacagca ccagccgacc cgccggaaca
     6241 ttacgtgccg ccagcagcgg cgccagcgcg cgcaatggat tggtaagtat attcgcgacg
     6301 accacgtcgt accgtgacgg gtcatcctgc tcgggcgtga cgaaatcggt ctcgacttcg
     6361 ttgcgcaagg cattgtcgcg cgcagcgacg atggctacgg gatcgatatc gacgccacgc
     6421 accgccccgg caccgagcag gcgcgcagca atggccagaa tgcccgaacc gcaaccgtag
     6481 tcgagcaatg attctccgcc acgcagctga ctgtcgatcc agcgcaggca caaccgggtg
     6541 gtcggatgcg atccggtccc gaaagccagg ccaggatcaa gatgcaggtt gatggcctcg
     6601 ggggcgggtg gcgtgtgcca tgacggcacg atccacagcc ggtcgctgac gtggatgggc
     6661 ttcaggtcag cctgggtggt acggatccag tcctgatcgg ccagcgtatc cacacgccag
     6721 tcaggcgggt gcatgccgat ggcggcagca aaggccgcca acagggcacc cgcgtcctgc
     6781 gtcgcatcga acagcgccac gatccggtta tgctcccaga gtacctgttc ggcgctgccg
     6841 ggctcaccga acaccggttg ctcatccgcc gaacccgctt cggcatcctc gatcgaagtg
     6901 gataccgcgc caaggtcaag cagaatatcc gaccactcgt ctgcaagctc tccgggaacg
     6961 gacaccgaaa cggccagcca gggcatggct cagttcccgc tcggtggggt cagtttgtgt
     7021 tcgagatagt ggatgctggt gccaccggtc agcacattct ggtcgcgcat caggtcctgg
     7081 tgcagcggaa tgttggtgtg aatgccttcg accaccatct ccatcagcgc cgtgcgcatg
     7141 cgtgcaatgg cctggtcacg cgtggcgccg tgggcgagca gcttgccaat cattgaatcg
     7201 taatggggtg gcaccacgta gttctggtac acatgtgagt cgacgcggat gccaggcccc
     7261 ccgggggcat ggtaggcggt aatgcggcca ggtgacggca cgaagctgta cggatgctcg
     7321 gcgttgatac ggcactcgat ggcgtggccg cgaaactgga tatcctcctg accaaacgac
     7381 agcggttcgc cggcagcgat gcggatctgc tcctggacga tgtcgatgcc ggtaatcagc
     7441 tcggtgaccg ggtgctcgac ctgtacacgg gtattcattt cgatgaaaaa gaactctccg
     7501 tcttcataca ggaattcgaa ggtgccagct ccacgatagc cgatgtcacg gcatgcctgc
     7561 gcacaacgct cgccgatgcg gtcgcgcagt atgcggtcga gccccggtgc gggagcttct
     7621 tcgagaattt tctgatgacg gcgctgcatg gaacagtcgc gctctccgag gtgtaccgca
     7681 tggccatgtt catcggccag cacctggatt tcgatgtgcc gcggacgttg caggtatcgc
     7741 tccatataca ccatcgggtt gccaaacgcc gtctgtgctt cagcctgggt catggccacg
     7801 gcattgagca atgcgccatc gctgtggacg acacgcatgc cgcgtccgcc accgccaccg
     7861 gctgccttga tgatgatcgg atagccgatt tcctgcgcaa tacggtggat ctcttccgga
     7921 tcttcgggca aggcgccttc ggagccgggc acgcacggca cgtgggacgc gcgcatggca
     7981 gtcttggccg acaccttgtc acccatcagg cgaatggttt ccggtcgcgg gccaatgaat
     8041 acgaaaccgg acgattcgac ccgctcggca aaatcggcat tttccgacag gaagccgtag
     8101 cccggatgaa tggcttcgga atcggtaagt tcggccgcgc tgatgatggc ggggacgttc
     8161 aggtaactca acgctgaggc tgccggtcct atacagaccg attcatcggc cagtttgacg
     8221 tatttggcat cggcgtcggc ttcggaatgg acggcaacgg tgcggatgcc catttcgcga
     8281 cacgcacgct ggatgcgcag cgcgatttcg cctcggttgg cgatcaggat tttttcgaac
     8341 atgacaggtc tgtgctcagc cgataatgaa cagcggttcg ccgtattcga ccggctggcc
     8401 gttttcaacc aggatggcct tgatggttcc agtgaattct gattcgattt cgttgagcag
     8461 cttcatcgcc tcaatgatgc agagcgtttc gccagccgac acgctctggc cgatttcaac
     8521 gaatgacttg gcaccggggt tgggtgaacg gtagaacgtg cccaccatcg gtgatttgac
     8581 ggtctgccct tcgataaccg gtgcagacgg tgccgtcgga gcagtggcaa ccggggcagc
     8641 aacctgcggt gcggggaggg tggccgcata ctgcatggct ggagctgcgg gtgcaaaacc
     8701 acgcgaaata cgcacctttt cttcgccttc ggtgatttcg agttctgcga tgccggattc
     8761 ttccaccaga tcaatcagtt ttttaagttt acgcaaatcc atgtaagtca gacctttttg
     8821 aaatgttggt tgagcgcgta gtcgagcgcc agctcatagc cggttgcgcc caggccggtg
     8881 atgacgccga cggccaggtc gctgaaataa gagtgttgcc ggaacggttc ccgtgcgtgc
     8941 acattggaca gatggatttc gatgaatgga atcgcaacag ccgccagcgc gtcacgcaaa
     9001 gcgatgctgg tgtgcgtcca tgccgcaggg ttgatgagaa tgaaatcgta gcggcccatg
     9061 gcgcgatgca cggcatcgat gagcaccggt tctgcgttgg actgaacgaa atcgagctgc
     9121 gccccggtct gcccggcctg ggaacgtagt ctggattcaa tatcgtcaag cgtgacgtgg
     9181 ccgtaaattc ccggctcacg ggtacccagc atgttgagat tggggccatt gagcacaagg
     9241 atacgtacgt tgttgtgaga agaacccgag gcggtcatca tggatgcaag tttgccgaaa
     9301 atgggccaaa atgtccagat attgaaagaa tttgtaaaat taacggcggt gggtcacgat
     9361 cggcacatgt gcctgcagcc aggagcgatc gatcgcaccg gagcgtgtgg ccaggatgcg
     9421 cccgtcagcg ccgatgaccg cggcaaaagg cagcgagtct ttgtagtcgc cgagctggcg
     9481 catcgagttg aaggtttcga cgtcgccgaa cagtaccggc cagtcaatgt ggtggctggt
     9541 gacgaagcgc tggaccgcag cccggtcgtc gagcgcgatg ctgattacct ctacctggcg
     9601 agcggacggg tcagtgtgcc acaggcggat ctgatccatc tgctgctggc atggcacgca
     9661 ccagcttgcc cagaaattga tcaccagcac ctgtccgctc cattgacgca acggctgacg
     9721 gatacccgcc agatcaacca gatggctgga gagcaacggg gcggccgtcg gtgcggccgg
     9781 cgtgcgattg gccagataca gccagccagc cgctgccaga acgacaagca acgacagtac
     9841 agtaatccag cgcattttgt cagacatgtc ctgctcccga ttgacgcaac acccgcagca
     9901 ccgccgcagg cggttgcagc ccgaccacac gctctggcag cagtcggccc gagcggtcga
     9961 ataccagcat gaccggtggc ccgaacacac cataacgcgc acgcagcgca tcatcctgtg
    10021 gcgagttggc agtcaggtcc gcgcgtaaca gtaccatgtg ctgcatctgt tgcgccactt
    10081 gcggatcacg cagcgtggta gcgtcaagtt ctttgcatga gacgcaccag tcggcataaa
    10141 aatccagtac cacacgctgc ccgcgtgcct gagccagtcg cgcgtccagc gtagcggcat
    10201 cggtcacggt gatgaacgtc gggccgtccg ccatggattc gctggcgctc gccgcgtgct
    10261 gcagcaccgc cagtggctga agcaggctgc gcgagccggc cagcacgccc agcaacaggg
    10321 cgatgccggc caccagcagc atgaccgcaa agcccttcca cagccgggcc cagccgccag
    10381 cctgcaccgg aagcgcatcg agcgcgtgcc acgagacggc cgacaggatc agcagcgccg
    10441 accacagcag catctggaca tggatgtcga gcagtggggc aatgagccag atagccatgc
    10501 ccagcatgag tacgccgaaa aactgccgga cgccatccat ccaggcgccg gcgcgtggca
    10561 gcaggctgcc tgaagtcaga ccgaccagca gcagcgggac gcccatgccg agagcgagaa
    10621 agaacagtcc ggccgcaccg agccagacgt tgccggtgcg agcgatccag gccagcgccg
    10681 ccgccagcgg cggcgctacg cacggtccga cgatcagtgc ggacagcgcg cccatcagca
    10741 gcgtgccgag caggctaccg ccgcgcatgc ggttgctcag gtcggaaaag cggctttgca
    10801 acgcattggg cagttgcagt tcgtacagac cgaacatcga cagcgccagg gcgacgaaca
    10861 gcagtgcagc cccgcccagt gcccaggcat tctgcaggac gttggacagc agcgtaccgg
    10921 acagcgccgc agctacgcct gcagcggcat aggtgaccga catgccgaac acgtaggcca
    10981 gcgatagcag gaatgcaccg gtgcgcgtat gctgcttctg gccgacaatg atgcccgaca
    11041 gcaccgggat catcggaaat acgcacgggg tgaaggccag caacaccccg gcgacaaaga
    11101 acgccgcaat caccgacaac acatggccgc tgcccagggc atggctgaac acgtcctccg
    11161 gggcggcgcc gcccgatgtt gcagggggtg tcgcgtcagg tgaagtcagt ggcgaaggcg
    11221 tgctgccgga cgcatttgcc gcaagcgaca ggctggccat gtggtgtacg ggcgcatagc
    11281 agatgccggc atcggcgcag ccctgggcga tgctttccag ttgcagggtg ggtgcggttg
    11341 catggtcgaa cggcagcacc acacgcacac tgtggcgata gatggtttcc agtccgaata
    11401 ccgggtcctg cttgatcgtg cccggcggca gttgcggtgt gcctgcggtg gccccactca
    11461 ggtggaattg cagtttgtcg cgatacaggt aacaacaggg gtcgacctgc caggtcactt
    11521 cgatgtgatg gctgtcgagc aggcgcgccg aactggtgaa cgcgcgttcc ggttggggaa
    11581 aatcttccgc gttcgcaagg gaaatcatgc ccaacagggc gaatgtcagc cacagcagac
    11641 gcatggtgca actccatttt tatcatgctg cgcatgagcg cagcgcgaga aaccgcgcct
    11701 gggcgcggtg caggcaatgc cgccgggacg gcaaggcgtt ccattataac ccgggcgtac
    11761 ggcatgtgcc atccgggagg gtctgcgtgc atgggcgggc gctcgcgtgg ccacggacaa
    11821 tctgggataa tgcggctttg catgacaatt gacaggatca tgggcgagac tttcgacatc
    11881 gttgtgctgg cggcgggcaa gggaacgcgt atgcgttcgg attaccccaa ggttctgcag
    11941 ccggtggccg gtcggccgat gctcgcccat gtactcgatg tggcgcgcag tctgggtccc
    12001 gtacggctgt gtgtggtgta cgggcatggc ggtgagctgg tgccgcaggc gcttgctgct
    12061 ccggacgtga cctgggtgcg ccaggatcgc cagctgggga ccggacatgc ggtgctgcag
    12121 gcgttgccgc actgcgcaac cggtggcctg acgctggtgc tgtatggcga cgtgccgttg
    12181 ctgcgcagcg aaacgctgcg tgcgctgctg gcacaggcgg ggccgggccg tctggcactg
    12241 ctgacggtgg aactggcgga tccgaccgga tacggtcgca tcgtgcgtga tacggccggc
    12301 cacatcgaac gtatcgtcga ggagcgcgat gccgacgcat ccatccgcag catccgcgag
    12361 atcaataccg gcgtgctggt cgcgccgacg gaaaaactgg ccgactggct gccgcgcatc
    12421 ggctgcagca atcaccaggg tgaatattat ctgaccgaca tcgtggccat ggctgcgacc
    12481 gaacggctgg acatcgttgc ctgtcaaccc gccgaggcac aggaaacgct cggcgtgaat
    12541 gatcgtgcgc aacaggccga agtcgaacgt gcgctgcagt ggcgtgcggc gcagcagcag
    12601 atggcctgcg gcgtcacgct gttcgatcct gcgcgcttcg atgtgcgcgg cacgctgcgt
    12661 tgcggtcgcg acgtggtgat cgatgccggt tgcctgtttg tcggcacggt cgagctcggt
    12721 gaccgggtac acgtcgggcc gcattgcgtg ctcgaagacg tgcgggtggc cagcgacgtg
    12781 cgcattgctg cgttttcgca cctgcatggc gcacaggtcg gcgcgggcgc gcaggtgggt
    12841 ccatatgcgc ggttgcggcc gggcacccgg cttgacgagg cagtgcacat cggcaatttc
    12901 gtcgaagtca agaatagtac ggtaggtgcg catagcaaga tcaaccacct gtcctatgtc
    12961 ggcgacagca gtgtgggcaa gcgcgtcaat atcggtgcag ggaccatcac ctgcaattac
    13021 gacggcgcca acaagcaccg tacagtcatc ggtgacgatg tattcgttgg ctcgggtacc
    13081 gaactggttg ctccggtgga actgggggag ggcgcgacca ttggcgccgg atccacgata
    13141 acgcaatatg caccggccgg cgagctgacg ctggcgcgcg agcgtcaggt gacacggccg
    13201 ggctggaaac ggccggtcaa gcaggcaaag gggagctgaa acatgtgtgg catcatcggg
    13261 gcagttgccc agcgcaatgt cgtgccgatc ctgcttgaag gattgcgccg gctcgaatac
    13321 cgtggctacg attccgccgg gctggcgatc gtgcgtgacg gagacctgca gcgactgcgc
    13381 agtctgggtc gggtggaaac gctggcggcg gccgtggcgc agcagggcct gcaaggcgaa
    13441 cgcggtattg cccacacgcg ctgggccact cacggtgtgc cgagcgagga caatgctcat
    13501 ccgcatgcga gtggcgatcg cgtggcggtg gtgcacaacg gcatcatcga gaatcacgaa
    13561 gcgttgcggg aagcgttgcg cgcagcgggc tatgtgttca gttcgcagac cgattccgaa
    13621 gtgattgccc acctggtgca ccagcattat gtgcagagcg cagacctgtg tcaggcggtg
    13681 cgccaggcga gctcccggct cgaaggcacc tatgccattg ccgtggtggc agccgatgcg
    13741 cccgatcggc tggtgtgcgc gcggcgcggc agcccgctgc tcatcgggct gggcatcgag
    13801 gaaaacttcg ttgcttcgga cgtgtcggca ctgctgccgg tgacacagcg catcatctat
    13861 cttgaggaag gcgatgtggc cgatgttggc cgctatgtca tccatgtcta tgaccgtgag
    13921 ggtgaaccgg tcgaacgtga ggtgtcggtg tcggaacagt ccgccgagat gaccgagctg
    13981 ggtaactggc gccatttcat gcaaaaggaa atccacgagc agccgcgtgc gctcaccgat
    14041 acgctgcatg atgtcgtcat gggcgaacag ctgtcgcccg gcgtgttcgg gccggacgcg
    14101 gcggcagtgt tcgcccagac cggttcggtg ctgctgctgg cgtgcggcac cagccaccat
    14161 gccgcccgcg tggcctgcta ctggatcgaa tcgctggcgg gcattcgctg ccaggccgag
    14221 atcgccagcg aataccgtta tcgcgacagc attcctgatc cgcaggcgct ggtggtgacc
    14281 ctgtcgcaat cgggcgaaac tgcagatacc ctggccgcgc tcaagcacgc ccagtcgctc
    14341 ggtcacgatc acacgctgtg catttgcaat gtgccggaat cggcgctggt gcgagcatcg
    14401 aagctgcgtt ttctgacgcg ggccgggcca gagatcggcg tggcttcgac caaggcgttc
    14461 accacccagc ttgccgcgct gttcgtgctg acgttgttgc tggccaaagc gcgtgggcgg
    14521 ctcgacgccg cgcaggaaca gtcgcacatt catgcgctgc gcgcgttgcc gggtgcggtg
    14581 cagcgggtgc ttgcgcttga agacgaggtg gcacattggg cggagcggtt tgccggaaaa
    14641 cagcatgcgc tgtttctcgg gcgtggcatg cattacccga ttgccatgga aggcgcgctc
    14701 aagctcaagg agatttccta catccatgca gaagcgtatc cggcggggga gctcaagcat
    14761 ggtccgctgg cgctggtcga ccgtgacatg ccggtggtcg caatcgcgcc caacgatgcc
    14821 ttgctggaaa agctcaaatc gaacctgcat gaagtcagcg cgcccggggg cgagctgttc
    14881 gtatttgccg atgccgatac gcatatcgct gaaggtgagg cggtgcacca gctgcggctt
    14941 gacgcgcatg ccggctggct ctcgcccata ctgcatacgg tgccgttgca attgctggcc
    15001 taccatgccg cgttgcagaa gggcaccgat gtcgataagc cacgcaatct ggccaagtcg
    15061 gtgaccgtgg aatgacgcca ttcgcccctc ctgcgcgcag cctgatcagc gccagtcgcc
    15121 accacgatat cagctgcggt catgtggtgt tcggtcacga gcacaagtgc cggcacctgc
    15181 acggtcacaa ttaccgcatt catttccgcg tcagcggcga gcgcg
//
LOCUS       DAIANL010000113        27921 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_142249, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000113 DAIANL010000000
VERSION     DAIANL010000113.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 27921)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27921)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27921
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142249"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5039 a         7869 c         9304 g         5709 t
ORIGIN      
        1 gcacgtaccg aggtggtgcg ctgtctgggt aacgacctgc aggtgccggc cagcgccgag
       61 atcgtgctgg agggagtgat tcatccggag gaaacggcgc tggaaggccc gtttggcgac
      121 cataccggct attacaacga ggtcgagcgc tttccggtat ttacggttga gcgcatcacc
      181 atgcgacgtg atgcgatcta ccacagcacc tataccggta agccgcctga cgaaccggcg
      241 gtgctgggcg ttgcgctcaa tgaggtattc gtgccgctgc tgcagaagca gttccccgaa
      301 atcgtcgatt tctatctgcc gcccgaaggt tgttcctacc gcctggcggt ggtgtccatc
      361 cgcaaggcct atcctggaca cgccaagcgt atccagttcg gtatctggag tttcctgcgc
      421 cagttcatgt acaccaaatt catcctggtg gtcgacgaag acatcgatat ccgcaactgg
      481 aaagacgtga tctgggcaat gaccacacgc atggatccgg tgcgcgacac gctgctggtg
      541 gaaaatacgc cgattgacta tctcgacttt gccagcccgg tgtccggtct ggggggaaag
      601 atggggctcg atgccaccaa caaatggccc ggtgaaacaa cgcgtgaatg gggtacgccc
      661 attgcgatga gcgatgcggt gcgcgagcgg gtggatgcgc tgtgggacca gctcggcctg
      721 tagttcccgg tcaggttgcc agccggcatc agcgcgtggc gcgaacaggt tgcgtctggg
      781 gcaacgcagt ttccgcgatc aactcgtgcg tccgggctgc gcgtgcatca atgaaccgtg
      841 ccctgccatt ttccaccgcc atggcgatgc cacatgggtc atgcgggcca ggataccgag
      901 gccgtggcac atgatgcacg ccatgcagat tgaaatgcag aatcagtcat gatccccaca
      961 gcggcagatg cagattgcgc gcgtagcgcg tgtcgtcggg cggcatgccg caatgtgggt
     1021 tgcatgatgc atcagtgaaa tcggcaggcc aagtctccgc atcgccagca gcgttcaacc
     1081 ggaacgtctc cgggcagaaa tcactccccg atacaccgcc acagcacagc ggcggcatct
     1141 gtgcgcatgt ctgattgcga tgctggttcg ggcagtggtg tatcagccac ccatccggtg
     1201 ctgttgatcg gatcggagcc gggcgctgcg gcgtgcggca ggtcattcga tggcagcgac
     1261 gaggcatgag ggaacgattg cgcgaccatg atatcgaatg gctgcataat ttggcttgca
     1321 tttagttctt gttagtacta tattgacttc aacgattggg aaaggcctgt acggagatca
     1381 tggacaaacc cgcttttttg ccattgctgc gcgagttggc gcgcacctat caggcttttg
     1441 agagttattc ggcccggcat gtccggacca tgggattgac ctcgtgccag tttgacatca
     1501 ttgctacgct gggcaatacc ccggggatga attttcgtga attgggcgag cgcacgctga
     1561 ttaccaaagg tacgctgaca ggtgtggtcg atcggctcga atccaggcag ttggtgcgtc
     1621 gtgtgccgga tcccgatgac ggacgcagcc agatcgtgca gttgactgct gctggagaaa
     1681 cgctgtttgc cgaggtgttt ccggcccata tcgaatacat gtcacaggca tttgcttcaa
     1741 tggaggaacg cgaactggct ggcgtgcgtt ctgcgctggc acggctgggc aggctgttcg
     1801 accctgtgga ggagacggca ccatgacagc tgcctatacg cgggtggcca tgttgctgca
     1861 ctggttgatg gccatgctga tactcggcac gtttccgctc ggatcgtaca tgagcgatct
     1921 gaaactgtcg ccgcaaaagc tgaagctgta cgcgtggcac aagtggatag gcataaccat
     1981 acttgcattg tggttggtgc gcgtcctatg gcgagtcagc catcgaccgc caccgctgcc
     2041 gcccggtacg ccgcgctggc aggaacgggc ggcagaaatc actcacagtc tgctgtatgc
     2101 gctgatgctg gcgattccgc tgagcggctg gctcatgagt tcggcgctcg gtttcacggt
     2161 cgtctggttt ggtgtcgtgc cgttgccgaa tctggttgcg cctgacaaag gtctgggcga
     2221 ggtgttgaaa caggtgcaca gcgcactcaa tgatggtttg ctggtgctcg tcagtctgca
     2281 tctggcggca gtggtgcaac atcagtttct gctgcgcgac ggagtgttgt ggcgcatgtt
     2341 gccgtggggg acgaaaaaat gaaatccggg tggaagttca tgcttgcagg catctgctgg
     2401 acggcggcgg cgcaaggggc gacgttacag aacgtggtgt tgccgcagag cagcatccgt
     2461 ttcgtgtcgc atcagatggg cgttgccgtg ccaggacatt ttgcccggtt tacggcgcga
     2521 gtgcatttcg atccggccgc agcgcacaac ggcatggcca gcatcgcggt tgacacggcc
     2581 agcgccgatg caggcggcaa cgaagtcgac gccacgttgc gttcgtcaca atggtttgac
     2641 agccagcact ggccgcaggc cacatttgtt tcgactgcgc tcaccgcgct gggtggcgcg
     2701 cgctatcagg ccagtggcct gctgaccatc aaaggcaaaa gccatcccgt caccgtaccc
     2761 tttgtgctgg aaaacatgcc cggcgggttg acggcgctca atggcacgat cgccatttcg
     2821 cgcgcccagt ggggcatcgg cgctggcgag tgggctgatc cgtcggtggt tgctgacgtg
     2881 gtgcaggtgc agtttcatat tgtagtgaag ccgtagcatc cggcggggtc cgatgcgtgg
     2941 gccgcagccg gcaatcccaa gcggtggggc ctgatggcct tgccgtaaac cctgaagtga
     3001 tattccgagg agcaagacga catgaaaaaa tcgttgctga tgttttccct gctggccgct
     3061 gtcagcgcca gcgcgttggc cgacagcact acctatacgt tcgatccgca ccatgtgtgg
     3121 ccgattttcg aggtcaatca tctcggtctt tcgacccagc gcggacgttt cgatgaaacc
     3181 agcggcacgg tgacgctgga tccggcagcg aagaagggca gtgtcaccgt gacgatacag
     3241 accaagtcgc ttgacatggg tttccctgaa tggaacgagc acctgagcgg acctgctttt
     3301 ttcaatgtgg ccaaatatcc ggtcatgacg tttaaatccg acaaacttga gttcgatggc
     3361 gaccgcgtgg ttggtgccga tggccagttc accatgctcg gcgtgacgca tccgttgcat
     3421 gtgacggtgc acaatttcca ttgcggagag catccgatga tgcacaagcc ggtatgcgca
     3481 ggagacatca ccgctaccat taaacgtacg gactatggca tgaacgccta cgtgccgatg
     3541 gtcggcgatg aagtgaacat tctcgtaccg gtcgaagcga tccgcaactg accgtatgtg
     3601 ctttcagcgg ttgttcccgc ccgctgcggg agcagccggt accagtatcc cgttttccag
     3661 gagaagaaca tgacccaacc cgtgtgtgca cagaaatccc cctatgccgt ggaattgcaa
     3721 cccggcgaat attattggtg cgcttgtggc cgctcgaaaa cacagccatt ctgtgacggt
     3781 tcgcatgccg gcaccgacat cacgccgacc gcatttaccg tgaccgagac caagaccgtg
     3841 tacctgtgcg gatgcaaggc gagcaagaat gcgccattct gcgatggcag ccatcaggcg
     3901 ttgtaacccg gaaacggcat catggctggc tcttgcgggc cggccattgt atgctggtcg
     3961 ggcatgagcc atttctcttt tcgtctgatc gcctggcaac agcattccgg ccgccatgac
     4021 ctgccatggc agggcagccg cgacccctat cgcatctggg tgtcggaaat catgctgcag
     4081 cagacccagg tgagcacggt cattccctat tatcagcgct tcatggagcg tttccctgat
     4141 ctgcctgcgc tggcggctgc cgacctggag gaggtgctac gctgctggag cggacttggg
     4201 tactattcgc gcgcgcgtca tctgcacgca gcggcgtgcc atgtcgtata tgaactgggc
     4261 ggggtatttc cgcgtgaggc catgcttgcc gtacaactgc cgggtgtcgg ccggtctacg
     4321 gcggcggcga tcgcggtgtt cagcgccgga gcgcgcgagg cgattctgga cggtaatgtc
     4381 aagcgcgtgc tggcgcggca tcagggcgtg cacggctggc cgggcgatgc ccaggtggag
     4441 gcgcgactgt gggagcttgc gcgcagcctg ctgcccgggc aggacatcga ggcgtatact
     4501 cagggcctga tggatctggg cgcgaccgta tgcacgcgcc gcgcgccgcg ttgcgcgttc
     4561 tgcccattgc aagcggactg tgtggcgcgt ctgggcgggc tggctgacca gctgccgacg
     4621 ccgcgaccgc ggattccggt acagcaacag cagttgcgcc tgatgattta ctcccatgcc
     4681 ggacgcgtat ggctggaacg tcgcccggcg ccgggcatct ggggcggatt gtggtgtttt
     4741 gcccgcggcg acagcgatga gcctggggcg caatgcgaac gcacctgggg tgttccggca
     4801 atccgggtca attgcggtga agtgttcagc cacacgctga cgcatcggcg actgcacatc
     4861 gagcctgtga tggtcgaatt gcgtgacgca ccgcggcaca cgtcggtggg ccgatggctg
     4921 gccccggacg aggcactgac aatggcgatc cccgtgccgg tacggcgatt gctgcaaccg
     4981 ctggcggccg ccgcggacag caagcggacg acgagtggcg cgcaatacaa tggaatgccc
     5041 gtgccacctg cggatcaggt ggggcggtaa tgtattcgta cggctgcggg aagaactgat
     5101 ggaattcatc gacagtcatt gccacctgga tgccacggaa tttgatgccg atcgcgatgc
     5161 agtggccgct cgcgcgcaag cttccggcgt gacgcaactg atcgttccgg cagtcgatgc
     5221 ggccgggttc gatacggtgc gcgcggtgtg tcagcgctat ccacagtgcc gtgcggcgta
     5281 cggtctgcat ccgatgtatg ctgatcgtca ccggatgcag gatgtgaccc ggctggatca
     5341 gtggttgcgc acggagccgg cggtggccgt gggcgagatc ggtctggacg gctccgaccc
     5401 gggtgctgat cgtgagcgtc agcgcgaatt tttcgacgca caactggcgc tggcggcgga
     5461 cctggatctg cccgtgctgc tgcacgtgcg ccaggctgtg gatgcggtaa tcgccagcct
     5521 gcgccgttat ggtttgcgtc gcggcattgc ccatgcgttc aatggcagcc tgcaacaggc
     5581 acagcaactg atgcgcatgg gattcctgct tggattcggc ggtgccatga cctggccggg
     5641 tgcgacccgg ctgcgcaagc tggccaccga attgccgctc gaatgcatcg cacttgaaac
     5701 cgatgcgccc gacatttcgc cgcaatgggc gcgtggcatg cgcaaccagc cggaatactt
     5761 gccacgcatc gctgccgagt tggccgggct gcgcggcgtg gacatcgtcg aggtggcgca
     5821 ggcgacaacg accaacgtgc gcaaggtgtt attccgtggg tgacgtacgc atccgtccgg
     5881 ccacgttgct gccgccgcca ctggtgtgtg ccgtcggtct tggcgctgcc tggtggcttg
     5941 aacgccgctt cccctggggg tggcagccac cacggggcgc gcacctgctg gcctggggcg
     6001 tgctggcggt cagtgtgctg ctcatgctgt gggctgctgc aacaatctgg catcaccgca
     6061 gcacggtcaa tccgtaccgt ggcgcatcat cactggtgac tggcgggcca ttcgccttca
     6121 gtcgcaatcc gatctatctg gctgatgccc tggcttatgc cgccgtaacg ctgctcatgg
     6181 gcagcgcctg gccgctgtgc ctggcgccgc tggtgtgggc cacgatgcgc tacggggtga
     6241 ttgcccacga agaagcgcat ttgcatgcca gattcggagc gcagtatcag gaatactgtg
     6301 cccgggtcaa ccgctggttc ggcaggcgcg gttcgcactg aaccgccgat gcaaggtgat
     6361 gctcacagtg ttgactggca ccgacaacgc tgtaagccag ttgccggaat ctgccttggc
     6421 aattttctga caagtttttc ctgttttgac aggaattaga aagcaatttg tcagaaaaaa
     6481 caaattcata cctttaacag gcttttggtg ccgattctgt tcggttccga acctacaaat
     6541 tccgactgat ttgttactct tgagctgtga tttgccagac ggcgatcaaa cataaaacag
     6601 ggagcgccgg ttttgcattc ccggggagac agccggaaca tcggccgtgc ggtgaatcgg
     6661 gatgtggtgt gcatggcatg cgatggatga agaaatagcc aagagttcag cggagcgacc
     6721 agccgccggc gccattgctg gaatggatga gctggcggcc gagctggcgc ggggcatgcc
     6781 tgccctgcgt gccctgctcg aattgctgta caccgaacca gacagacagc agcttgcatt
     6841 gctgctggcg cgaggcgacg acatcctgta ccgcatcgat ccggtcgtgc tggtcagcct
     6901 gtgtggcgac gggctcgggt cacttcgcct gccggtgcag cacaacggca aaaccctgtc
     6961 cccacaggat ggtgcgatcg gatccgtgaa cggcgtgtcg tttgcagccg cgatggcaga
     7021 tactggccgc aaccgcaccg agttcctcgc aacgcatgat tccatgaccg ggttacccaa
     7081 tcgggtgctg cttgttgagc gcatgcgcga ggccacggca cgtcatgcgc gtgatggcag
     7141 cagttttgcc ctgctgtttg tcgatctgga cgatttcaag cgcgtcaatg acaatgtagg
     7201 ccacgcagaa ggtgaccggc tgctgcggga aattgcggcg cgactgagcc agtcggtgcg
     7261 cgagatcgat ctggtggtgc ggtatggcgg cgacgaattc gtggtgctgc tcgatggcgc
     7321 cggcatcgag caggcgcgcc agaccgcgca acgcgtgtcc gaggaactgc tgcggccgtg
     7381 cgtgctgggt ggtcggctgt atcacccggg cgcaagcatc ggtgtcagcc tgtatcctga
     7441 agatggcgcc gatcccgagg cgctgctcat gagcgcggat cgggccatgt accaggtcaa
     7501 gctgagcggc aaacgcgcct ggcagtttca tacgcgcgaa ttgcgcgcac agacggaagc
     7561 gcgtattgcg ctcgaggccg ggttgtacaa atccatcatt gccggggaac tgcagttgcg
     7621 ctggcagccg cagttcgatg ccctgtccgg cgacatggtc ggtgtggagg cgattccgta
     7681 ctgggagcat ccgcacgacg gctgggcacc gtatcattcc tggcgaacac ttgccgaatc
     7741 gatcggcatg acagaagata tcgatgcctg gtcgctggcc gaagcgtgtg gtcaggtgaa
     7801 ccgctgggac agcgagcagg tggtgattcc gcgagtgtcc ctcaatttgt cgctgatcgg
     7861 tttcgaacgc ggcgaaatgg cgcaaacact ggcacaggtg ctgcagcaac agggtgtatc
     7921 cgggcgacgc atgatgatcg aggtggatga agctgcgctt gcctgcaatc cgtcccggct
     7981 cgaggcaggg ctgcgtgcca ttgaagcaac cggtgccggg ttgtcgatcg atggctttgg
     8041 caatcagggg gtttcgcttg tgaaattgcg acggttgccg gtacatgaag tgaagatttc
     8101 ctcggagctc gtggttcagg caacgatgca ggatgacgcg gttgacgtgg cgcgggcgat
     8161 cgtcagtctg gcgacgacat ttggttacgg cgtcgtggcg agcggggtgt cgagcgaggc
     8221 ccagcagcac ctgatgcagg ccatcggctg ccacgtcctg cagggcgact ggcaagctgc
     8281 ggccatgagc gcaagtcagc tggccgaaca gttgcgccat caataacgac cattcaggca
     8341 gaaagactat gtatcatcag caattcttgc gacacaaccg gattctggct gcgttgcaga
     8401 ccgctgaact cgaacgggtg cttccatatc tgcagtgggt tcggctgggt gcaggcaccc
     8461 aggtcgccat ggccggcgaa acgccgcgtc atgtgtattt tcccgtggat ggaacgattg
     8521 ccctgtttca ccagatggag aatggttcgt ttaccggtgt ggcagtgatc ggcaacgaag
     8581 gcatgttcag cgtggcgcac gtgctgggag ggaaaaccat cccgtatgag gccgtagtgg
     8641 aaaccccggg gcatttcttc aaggtcgatg ccaaaaccgt cacgctgctg gtagcgcaga
     8701 tgcaactgtc ggtcacgacc atgttgctgt acgcctaggc gacactgacg caatttgccc
     8761 aatcggtgat ctgcagcaaa cagcattcgc tgtaccagca attctgccag ttgatgctga
     8821 tcatcagtga caagagttgc tcgaacgaat tcgaaatgac gcaggcggcc attgccacca
     8881 ttctgggcgt acgccgcgtc agcgtgaccg aagttgcgca ggatctgcgc acccagggca
     8941 tcatcgacta tattcgcggg catgtgcgca tcaacgaccg tgacgggctc aaggggcgtt
     9001 gctgcgagtg ttacgaggtg gtgtcgaaag agttcgaccg gctgctgggc cggtcgaact
     9061 gtccgtgaag cgggcggcca ccgctcaggc ggtcgcgccc tggaacgaga acgcgtcggc
     9121 gaagaatgcg tcgtcgggca ggttgcgttg ctggcacatg cgccgtgcgg catcgaccat
     9181 tgccggcgcg ccgcagacgt agacatcgaa gcccgacaga tcggggtggt cctgcgccac
     9241 ggcgtcggtg accagcccgc gtcgcccttc ccactggtcg gcgtcggccg gttgcgacag
     9301 cacgggcgtc cagcgcagat gctcatgttc ggcggcccag cgcgcggggg tatcggcaag
     9361 atacaggtcc ttgcgcgcgc gcgctcccca gtacaaggtc atgggacggg tctggcggtg
     9421 gtgcagtgca tgcgagatca tggcctggat cggagcgaat ccggtgccgc cagcaatgaa
     9481 cagcagcggc cggtcactgt cctcgcgcag ccagaaatcg cccagcggac cctcgatgcg
     9541 caggatgtcc ttttccttca tgccatggaa tacatggctg gtgaacatgc cctgcggcac
     9601 ttcacgtacg tgcagctcga ccagcgcgtc gtcgtgcggt gcattggcga tcgagaagct
     9661 gcgccgctgg ccgtccttga gcaggatgtc gatgtattga ccgggcagga actgcaaccg
     9721 ttcgttgctc ggcagcttga gaaagatgcg catgacgtcg tcggccagcc gttcaaggcg
     9781 gtggacgcgg cacggcagcg tgcggatctg gatgtcgcgt gccgcgccga tttcgcggca
     9841 ttcgatgctg atgtcgctgg tggggtgggc cacgcagaac aggattttgc ccgcctcgcg
     9901 gtcggcatcg gtcagcccgg tggccgaacc gctgtgggtg acatgtcctt cgagcagcct
     9961 gcccttgcaa ctgccgcagg cgccattgcg acagccatag ggaaggttga agccttcgcg
    10021 caatgcggcg ctgagcacgc tttcatcttc ttccatactg aagtgatgac cactgggctg
    10081 gatcgtgacc tggtgcgaca tgatgggggt tcctgaaaat gtgcgggaca gcaaatacag
    10141 atgatgcaag actgttgaat tatccggatt tgcgacgggt tgttcaactg gcaccggtgc
    10201 gcagcgcgcg cgccagcacc cagcattcga tgcgttgcgc accgcactcc gacagtgcac
    10261 cggacagctg cgccaggctt gcaccgctgg tcatgatgtc gtcgacgatg gcgatattca
    10321 gtccctgcac gcacggattg cagacaaagg cgcggcgcag gttgctgcgt cgctgcgtcg
    10381 gggtgagccc ggcctggggt cgggtgtcgc gcgtcctgcg gcaggcgtgg tgcaacaggg
    10441 gtacgcctaa ccggcccgac agtgcgcggg cgatcagcag gctctgattg tagccccggc
    10501 tgcgttgccg cgcacggtgc agtggcatgg ccagcagtgc gtcgggcagc ggccttgcgg
    10561 cggcgagctg agccagtggt tcgaccagtg gctcgatcac accgaagtgg tggcggtact
    10621 tgagcgcctg gatcatgcgg tcgagcggga agccatagcg ccaggcggcc agcgtgcctt
    10681 gccagggggg tagatgtcgc agacactcgc cgcatggatg ctgcgcgccg ccaggcaggg
    10741 gttgggcgca caacgggcac agcgggccgt caagctgcgg cagggtggct tcgcagcgcg
    10801 gacacaggct gcggcgtccg catgcggcag cgcacagcag acagtcttgc gccggtacga
    10861 actgattaaa aaatgatctc atgttcaata attcggacaa gatgcttgac ttgttgcccg
    10921 ggcatgcaca caatctcgct gtttaccaca gcaggaatcg tgcatggcaa ctcatgttgt
    10981 tacggcaaaa ggatcgcttc ttgccgccag gctgttcaat attgccagca tggtcgcgac
    11041 ggtgttcgcg cccttgctga tggtctggat tgcggtatcc atcttcatgt acgcatcgat
    11101 cgcgcaccac ccgaatccac gcacagtcta ctataaccgc tgggccggct atcgttttta
    11161 cggcgcggct ggcacgatgg tcgctgcggg gcaaccgatt tatgcgttgt tcgacaactg
    11221 gcgcgggctg gtgctgatct gggtgctgat gatgctggtg gtggtgccgt ggggactgcg
    11281 cgacatctgg cgtgccggca aggaggagtg gcaggatatg accatagagg tggaaaaccg
    11341 tgaatgacat gaagcagccg gaaacgaccg gccgtacctg gcgcatcggc gaaatcgagg
    11401 cattgttcga cctgccgttc aacgatctga tgtttcgcgc ccagacggtg catcgtctga
    11461 atttcgatcc caatgcggta cagttatcga ccctgttgtc gatcaagacg ggtggctgtg
    11521 ccgaagactg tggctactgt ccgcaatctg cgcatcatga tgcagactcg ccgcgcgaag
    11581 cgatgctgac caccgatgcc gtgcttgcag cggctcgcgc cgcgaaagcc gccggcgcga
    11641 gccggttctg catgggcgct gcctggcgca gcccgaagca ggacgatctc gaggccgtga
    11701 tggagatggt gcgtgaagtc aaggcgctcg gactggaaac ctgcgccacg ctgggcatgc
    11761 tcaaggcagg ccaggccggg cagctcaagg atgccggact ggactattac aatcacaatc
    11821 ttgatacggc acccgagttc tacggcgaaa tcattcatac ccgcacctat gcagaccggc
    11881 tcgatacact ggagcaggtg cgtgatgccg gactcaatgt gtgttgtggt ggaatcgtcg
    11941 gcatggggga aacgcgtgcg caacgtgccg gtctgctggc gcaacttgcc aacctcgacc
    12001 cgcagccgga atcggttccg gtcaacatgc tggtgcgtgt cgaaggcacg ccgctgcaag
    12061 acgtcgaagc actcgatccg tttgaattcg tgcgtaccgt ggccgtggcg cgcatcctca
    12121 tgccgcacag ccacgtgcgc ctgtcggccg gtcgcaatga aatgtcggaa gcggtgcagg
    12181 cgctgtgttt cctcgccgga gccaattcga ttttttatgg cgagaagctg ctgaccacgg
    12241 ggaatccgga cgtggcccgc gatcaacggc tgtttgacaa gctggggctg gttgccgagc
    12301 ccgctgctgc gcacggatga ccatcagcgc atcgctgtcc cgattgcgcg cagggctgtc
    12361 ggaactggct gatgcacacg cgttgcgccg caggcgggtg ctcgaatctg cgcagggcgt
    12421 gcgcgtgcat gtggacggcc gtgactggtt gtcgttctgc agtaatgatt atctcggcct
    12481 ggccgctcat ccggcgctga ccgatgcgat ggttcgtgct gctggcgagt atggcaccgg
    12541 cgcaggcgcc tcgcacctgc tgtgcgggca ccatctctgt cacgagcggt tcgagagcga
    12601 gtttgcccgt tgtgtcggca tgccgcgcgc gctgctgttt tctaccggct acatggccaa
    12661 tctggccgtg gtgacatcgc tggtcgggcg tgacggatca gtgtttgccg accggctgaa
    12721 ccatgcttca ctcaacgatg ctgccgtgct gtcgcgcgcc cgcctgcacc ggtttgcgca
    12781 cggtgatctg gatgcgctcg accggctgct ggcggcacat cctggcgcag acaagctggt
    12841 agcggtggat ggcgtgttca gcatggacgg cgacctcgcc aatgttccgc ggctgctgga
    12901 attgtgcgaa cggcacgatg cctggctgct ggtcgatgat gcccatggct ttggcgtgct
    12961 gggagagcag ggccgcggca tcctgtccca tttccgcctg cattcgccac gcctgattta
    13021 tctggccacg ctgggcaagg cggccggggt ggccggtgcc gtggttgccg gagagaccga
    13081 gctgatcgaa tggctggtgc agaaggcgcg cacctatatt tataccacgg cccagcctcc
    13141 ggcattggcg gcagcattga gcgagagcct gcgtcagatc accgctgcgg acggtgcgcg
    13201 cgcgcacctc ggacggttgg tcgctcattt gcgcaacggc ttgcaggcgc agggctggac
    13261 gctgggtccg ggcacgacgc cgatccagcc ggtgatcgtc ggcggcaatg ccgaggtgct
    13321 ggcgttgagc gcgacacttg aacaatgggg tgtgctggtg ccggcgatcc ggccacccac
    13381 tgtgccggag cgcagtgccc gcctgcgcat cacgctgtcg gcagcgcatg ccgaggcgga
    13441 tgtcgacctg ctgctgtccg caatggcgca agcggggcca tgatgatgaa aacagacggc
    13501 aacaccgtga cgaatgccgc cgtgccgcca ctggtgctgc tgcacggctg gggcatgcat
    13561 ggcggcgtgt ggcaggggct ggccgcacgc ctgcagcagg agcggcaggt attgatgccg
    13621 gatatgcctg gctatgcggc gcaaccacag ctcgatacct gtacgctgga atcgctggcg
    13681 gagcacatgc tggctgactg gcccgcacag gtggacctgt gcgcctggtc gctgggcgga
    13741 ctggtcgcat tgaccatggc gcggattgca ccgcagcgta tccgccgtct ggtgctggtt
    13801 ggcagtacgc catgcttcgt ggaccgcccc gattggaact gcggcatgcc ggcagcggta
    13861 tttgcagggt tcgctgatgc gctgcagcag gagtatgagc cgacactgcg tcgtttcatt
    13921 gcgttgcagg cgcagggcga tgctgctgcg cgcgaggcgc tgacggtact gcgggcgcaa
    13981 ttgttcgcgc ggggacggcc cggagtggcc accttgcgtt gcggattgga tctgttgctg
    14041 cgcagcgatg tgcgcgccag cgtctcgcag gtgatggtcc cgacgctcgt ggtacagggc
    14101 aaccacgacc ggctggttcc accctgcgcc gcacactggc tggcggcacg cctggaacat
    14161 ggatacctgc tcgagatgcg tgctgccgga catgcgccat ttctgtcgca tgttgacgaa
    14221 tttgcacgcc atttgcagga atttttgtcg gagtgaacat ggaagaggcg cacaatgcgc
    14281 aggtcgacaa acgcctggtg cgacgctcgt tcgaccgtgc ggccgcggat tacgatgcac
    14341 acgcagtgtt acagcgcgaa gtgggcaacc gcatgctgga acgactggaa tacattcgcc
    14401 atgagccacg ggtgattctc gatgccggcg ccggcaccgg cagcggcgca agcggtctgc
    14461 aggcacgcta tgcggatgcg cgcgtcgtgg cgctcgatct ggccgagtcg atgctgcgta
    14521 tcgcctgccc ggtacggcca ttctggcagc gctggcgcaa tgcaccggta ccggtgccgg
    14581 tgtgtggcga catcgatcaa ctgccgctgc gtgcagacag cgtgaatatg gtgtggtcga
    14641 gcctggcgct gcagtggtgc aacgatctgt ccgtaagcct ggcagaattc cggcgcgtgc
    14701 tggtgcctgg tggcctgctg atgtttgcca ccttcggtcc ggatacgctg aaggagttgc
    14761 gcacggcgtt taacgcggta gatggctatc gacacgtcaa tcacttcgtc gacatgcacg
    14821 acatcggtga tctgctggtg cagggcggtt atgcagcccc ggtcatggac atggagttca
    14881 tcaccctgac ctatgcaggg ctgtcggaga tgttgcgtga aatcaaggcg atcggtgcgc
    14941 ataacgtcac tgcggggcgc aatccggggt tgaccgggcg ggcacgctgg cacgcactcg
    15001 aacaggcgat ggaaacattc cggcagaacg ggcgtttgcc gctgacctgg gaggtcattt
    15061 atggtcatgc ctggaaaccg cttgaagcgc cgcggcgcac ggatgttcag cccgtagtct
    15121 ggcagtcgcg taaatcatga cggcggggtt tttcattacc ggaaccgata ccggtgtggg
    15181 caagacgcat gccacagccg ccctgctgcg cggctggggg gcgcagggga tgcgcgccgt
    15241 cggcatgaag cctgtggcct gcggcgcagc agtacacgag ggtcgggtat tctgggaaga
    15301 cgtcgactgc ctgcaggcgg cgggcaatgt cgatgcgccg ctggcgctgc gcaatcccta
    15361 ccgttttgaa gtgccggtgg cgccgcatgt ggcagcgcgg gcggcgggct gccggctgga
    15421 gctaggcgtc atccgtacgg cgtatgatgc actggcggcg caggccgatg tggtggccgt
    15481 tgaaggggcc ggcggctggt atgtaccgct cgatgagcgg catacgctgg ccgatgtacc
    15541 gcgtgaactg cagctgcgag tcattctggt ggttggcatg cggttggggt gcatcaatca
    15601 tgccctgctg accgggcgcg ccattcgcgg cgatggtctg gaactggccg gctggatcgc
    15661 caattgcgtc gatccgggca tggctgcgtt cgcggacaat ctgcaggccg tgcgcgagcg
    15721 gctggaggct ccattgctgg gcgttttgcc ctggcgcgat gatccggcgg ctgaaaggct
    15781 gcattttgta acacatgcgc catcagggtt gtaatcaacc cgcatcggac tgcaaatttg
    15841 gggtatattt gagtcagagg ggccgatttt gcatctggca gggcatggat gcggtgcggc
    15901 ttcatgattt cctccagtcc ggtggcatgg caggctcggg tgcgcggcaa tcgtgccgct
    15961 gcatgtcgtg aatgcggacc gtcaggctgg tttggcaatc cgtcccatgc gtcttctggc
    16021 ttgaatgatg aatcgtctgt ttcgttattg gcagcatctc gggttactgc agcgtctgat
    16081 gtggctgatc gtggtcacca acctcagcgt gctgctgtat gcggttgtgc cgttgccgcc
    16141 gcttggcctg ctgcccctgc tgctgttcgt atggtgggtg agccatgttt tgttgagccg
    16201 gagtctgggc gcgctcgggc gcctggtcac tgcggtcgat gcaatgaagc agggcgacta
    16261 tgaggttcag atcgacgtgc agggcgcgcc ggaattgcgc atgctgggcg ttgcattcaa
    16321 tgacggcatt gcgcgcgcca aacgtctgat cgtcgagttg cgcagccggg aagcccagca
    16381 gagcgaattg ctcgaacgca tcactgcgca gaatttcgct tttcgcgagg agcggcgctc
    16441 gatcaatgcc gcagtgatcg ttgccgaaac cgatgtgcag ggcaatatta cctatgtcaa
    16501 tgacaagttc tgccagatca caggctatgt gcgtggcgaa ctgctcggca agaatcacag
    16561 catgctggga accggggggc tggggcagac gggctaccgt gcgttgtggg cgacgatcaa
    16621 cgcaggcaat gtctggaccg gtgagttcca taaccgcacc aagcgcggcg acgggtactg
    16681 ggtgcagacg acgatcgtgc ccattctgga tgatgccacg cgcaagccgc gccatttcaa
    16741 ggccatcagc atcgacatca cgccgcgcaa acagctcgag gaattgttgc accaggaacg
    16801 cgagcgtgcc gaaatcacgc tggcttcgat cggtgacgcg gtgatcagta cggatgtgca
    16861 gggcaacatc accttcctta acgaagtggc agaaacgttg accggctgcc ggctcgccga
    16921 ggcgaaagga ctggccgtgg cgcaggtgtt cgacctgctg cacgaagtga cccacgctcc
    16981 ggtgggaaat ccggtcatgc aggcgctggc agaaaagcgc aacgtcaaga tcgttgacca
    17041 tgccgtattg cgtgcgcgca atggtcagga gttcggcatt gaatgcactg ccgcgccgat
    17101 cgtcatgagc aatggccagc ttatcggtgc agtactggtg tttcatgacg tgagcgagaa
    17161 acgccggctg atgtcggccg tgcagtggca ggccggacac gatgcgctga cgcgcctgcc
    17221 gaaccgtaac ctgctcaacg acagcatcct gcgcgcgctg gcggaagcgc ggcgcaacga
    17281 aaccttgacg gcggtgtgtc tgctggatct ggacgatttc aagccagtga acgacaccta
    17341 cggccatgag accggcgata acatcctgat tgaactggct gaacgtctgg tcgtgacgtt
    17401 gcgtcaccag gacatggtag cccgtcttgg cggggacgag ttcgtattgc tgctcaacgg
    17461 gttcagacag gtggacgaga tcgagccgct gcttaaccgc ctgcttgagg tgattgccga
    17521 accgttcctg tttggtaata cggccgtgca cgtctgtggc agtatcggca tcacctttta
    17581 tcccctggac gatgccgatc cggacacctt gttgcgtcac gccgaccagg ccatgtacca
    17641 ggccaagcag tcggggcgca accgctttca cctgttcgat ctgctcggcg accagcaggt
    17701 gcagagcagt ttccgccaga tcgagcgcct gcgtcaggca ctgtacaatg atgaattcct
    17761 gctgtactac cagcccaagg tcaacatgcg caccggtcgg gtcattggca tggaagcgct
    17821 gctgcgctgg ctgcatcccg aacgcggcct cgtgccgccg caggatttcc tgccggcgat
    17881 cgagcagaac gggttgatcg tcgaggtcgg cgaatgggtg atcgaacagg cgctcaggca
    17941 aatcgaatac tggctggaag ccgggtatgt gtggcatgtc agcgtgaata tcgccggttt
    18001 gcattttcag caggccgatt tttgcgaacg cctggcgctg ctgttgtcac gccatccact
    18061 ggtgccgccg cagctgttgc agatcgagat tctcgaatcg gccgcactgg gcgaactcga
    18121 ccatgcgcgc ggtaccgtgg cgagctgcca ggcgttggga gtgagttttg cgctcgatga
    18181 ttttggcact ggctattcgt cgttgaatta tctcaaacac cttgatgtgg atgcgctcaa
    18241 gatcgatcag gggttcgtgc gggacatgct ggtcgatcgc gccggcctgt cgctggttga
    18301 ggcagtggtg agcatggcca gcgtgtttgc gacccaggtg gtggccgaag gtgtggaaac
    18361 gaccgaacac ggcgtgctat tgatccggct gggctgcgat ctcggtcagg gttatggcat
    18421 tgcccgaccc atgccccccg agcaggtggc gccgtggtcc gagcagtacg taccggaccc
    18481 gtcctgggcc atgtgggcca atacacactg ggagctgtcc gattttccgc tgctggtggc
    18541 gcagtatgat catctgaaat ggatcaaggc ggtggtggcg gtggtgtatg acgcgccgct
    18601 ggccatttgc cagagcgaat tgcatgacca tcaccaatgc cgtttcggca actggtatta
    18661 cgggccgggc cggaagcgtt atggccatct gccggaattt gccgtgctcg aaaccacgca
    18721 tatggaggtg catcgtatgg gcatggagct ggtgcgcctg taccatgcgg gcgagatcga
    18781 gcgcgctcgt gcgctgagcg aggaactgat gcgactcaaa gaccgggtgc tgcaactgct
    18841 gaccgatctg cagcacgccg tgatgagcga ggtggtgcat tgaaccgcgc tgcgggggcg
    18901 tggctggcgc tggctgcgtt actggctggc ctgctggcgg ggtgcgcggt cgcgccaccg
    18961 cgtccgctac cggctgcggt agcgccggtc gtgccacggc tgcatgaggt tgcctggaac
    19021 gagctgcccg gctggcagga ggatgacctg ctggcagtct ggccggcctg gctggcggat
    19081 tgctcggtca tgcgcatgcg gcccgcgtgg cgaccggcct gcgatgctgc ggccaggctg
    19141 gagccatcca gccgcgatgc ggtggccgca tatttccaga cctggttcag cccgtggcag
    19201 agcctgcagc ccgacggtgc ggcgcagggg ctgatcaccg gttattacac accgctgttg
    19261 cacggcagcc tgaaacgtga gccaccatac gtcgtgccgc tgtatgcgca gccgcacgac
    19321 ttgctgacag tggatctcgc cagtgtctat cccgagctga agggaaaacg actgcgtgga
    19381 cgattgcagg gggaccgggt cgtaccttac tggagccggg cacagattga cgccgatccg
    19441 caactgctgg cgggcaacga actggtgtgg gtggatgatc cgatggcggc gtttttcatg
    19501 cagatacagg gttccggaca ggtagagctg cctgacgggc agcagctgac gctgggctat
    19561 gccaaccaga acggctttcc ctaccgtgcc atcggaaaat tgttggtcga gcgtggagaa
    19621 ttgccggcat cacaggtgtc catgcagtcg attcgtgcat ggggcaaggc gcatgcgcaa
    19681 gagttgccga gcgtgctggc aggcaatccg gcctacgtgt tcttccgccg gctgcagggg
    19741 ctgccgcctg gcgcgatggg tatcccggtc gtggccgggc gcagcatcgc cgtcgatccg
    19801 cgtgcgctgc cgctgggtgc gccagtctgg ctgtccacgc gccagccgaa cggtgacgcg
    19861 ccactcaatc gtctggtact ggcgcaggat accggcggcg cgattcgcgg caatgtgcgc
    19921 gccgatctgt atttcggatt gggtgatgcc gcgggggagc aggccggcgt gatgcggcag
    19981 cctggcagcc tgtgggtcct gttgccgaaa ccggtgagcg catccgggct ggtgcgtcgc
    20041 tgaggacgca cgcagccacg gtcagcgacc gctgcgcatc gagtcgaaga actcggcgtt
    20101 ggtcttggtg gccttgactt tgtcgaggag gaattccatg gcgtcgagat cgtccatggg
    20161 atagagcagt ttgcgcagga tccagatttt ctgcaggatg tcgctcttga gcagcagttc
    20221 ctcgcggcgg gtgccggacc ggttgacgtt gatggccgga tagagccgtt tttctgccat
    20281 acgccggtca aggtggattt ccatgttgcc cgtgcctttg aattcttcga agatcacatc
    20341 atccatgcga ctgcccgtgt cgaccagcgc ggtggcgatg atggtgagcg agccgccttc
    20401 ctcgatgttg cgcgccgcac cgaaaaagcg tttcgggcgc tgcagggcgt tggcatccac
    20461 accgccggtg agtaccttgc cggaagacgg gatgaccgta ttgtaggccc gggcgaggcg
    20521 ggtgatcgaa tcgagcagga tcaccacatc cttcttgtgt tctaccagcc gcttcgcctt
    20581 ctcgatcacc atttcggcga cctggacgtg gcgggtggcg ggctcgtcga aggtggacgc
    20641 cacaacttcg ccgcgcaccg agcgcgtcat ttccgtgact tcttccgggc gttcgtcaat
    20701 cagcagcacg atcatcacca catccggatg gttgctggtg atggcgtggg caatatgctg
    20761 cagcatgacg gtcttgcccg atttcgggct ggcgaccagc aggccgcgtt gccccttgcc
    20821 aatgggcgcg atcatgtcaa tgatgcggcc ggtgatgttt tcctcggcgc ggatgtcgcg
    20881 ttcgagcagc aggggttcgg tggggtgaag cggagtcagg ttctcgaaca ggatcttgtt
    20941 cttggcattt tcgggcggct cgccgttgac gcggtcaacc ttgaccaggg caaagtagcg
    21001 ttctccatcc ttggggatgc ggatttcgcc ttcgatcgaa tcaccggtat gcagattgaa
    21061 gcgacggatc tgcgatggag agatgtaaat gtcatccggg ctggcgagat aggaggtgtc
    21121 gggcgatcgc aggaaaccga atccgtccgg caggacctcc agcgtgcctt cgccaaagat
    21181 gctgacgcct tttttcgcct ggtttttgag cagggcgaaa atcagctcct gcttgcgcaa
    21241 ccggttggcg ccctcgatgg tgttggcggt tgccatttcc accagttggg taacatgtag
    21301 ctgtttcaga tcggatagat gcatagggaa aactctcacc ctgcagcggg cggaataatg
    21361 aaagaatgat gatggggaat gcgcctgagg ctcgaccgta atgcggtacg atatttttga
    21421 cctgcttgtc gtcagcaggc tgtcgaaaaa cggttacgca ggtcggtatg cttcatttcg
    21481 tcctgcccgg caccatgctg gctgggtgtt gttccgggct ggctgcgtca ctcgcatttc
    21541 caaactgcct gtcggtccga ggcggctcaa gcgcagagta tctgaatcag atgttgctgt
    21601 caacaaaggc ggtcaattgg gacttggaca gtgcgccaac cttggtggct tccacgctac
    21661 cgttcttgaa gatcatcagc gtcggtatgc cgcgaatgcc atatttgggt ggtgtggcct
    21721 ggttttcatc gatattgagc ttggccacct tgatgcggcc ggcatactca cgggcaactt
    21781 cgtcaaggat gggggcgatc atcttgcatg gaccgcacca gtcggcccag tagtctacga
    21841 ttaccggtaa cggcgattgc aggacttcct gctcgaacgt ctcgtctgtg acaaagtgga
    21901 tatgctcgct catggggttc ctcacggact gtattcttgt aaaacgactt aaggtggatt
    21961 gaacacgcga atgcgcaaaa ggttgctgta tgctaaccaa aatctttcgg cttgtgaagc
    22021 ataactgctg aaatgcagcc ggaggctcag gactggacgg tttgcgtccc gctgctacaa
    22081 tggctgccgg acactggaga acccgatgta caggcaatcc ctgcaggacc gtagccagtc
    22141 ggtggtctgg cacccatgca cgcagatgaa acagcacgag gcactgccgc cgctggcgat
    22201 cgaccggggg catggcgcct ggctgtacga ccgtgacggc aaacgttatc tcgacgcgat
    22261 ctcatcctgg tgggtcaacc tgttcggaca tgccaacccg cgcatcaacg cggcgttgcg
    22321 cgagcagatg gaacgcatcg agcatgtgat gctggccggt tgtacgcatg agccggtggt
    22381 ggcgctgtcc gaacgcctga cgcagcaggc tggactcgcg catgccttct atgcatccga
    22441 tggcgcatcg gcgaccgaaa tcgcgctcaa gatgagcttc catgcgtggc gcaaccaggg
    22501 tgaagtggaa aagacccggt tcgtcagcat caggaacggt taccatggcg aaacgatcgg
    22561 tgcgctgtcg gtcaccgatg tggccatctt ccgcgatgtt tacgcgccct tgctgcgcca
    22621 gggggcgcag gcggccagcc cggactggcg gctggcgcag ccaggcgagt cggccgagca
    22681 ggtggcgctg cgtgcggcag ccgatctgga agcgtgtttg cgccggcagg acgggcgtgt
    22741 ggccgcggtc atcgtcgaac cgctggtgca gtgcgcggcg gggatggcca tgcatcatcc
    22801 ggtctatctc gcccgggtgc gccagttgtg cgacgaatat cgcgtgcacc tgattgcgga
    22861 tgagatcgcc gtggggttcg gtcgcaccgg cacgctgttc gcctgcgaac aggctggcat
    22921 cacaccggac ctgatgtgtc tgtccaaggg cattacagcg gggtatctgc cgttatcggc
    22981 cgtgctggcc agcgagacca tctaccaggc gttttatgcc gaagagatgg cggcggcctt
    23041 cctgcattcg cattcctata ccggcaatcc gctggcgtgt cgcgcggcgc tggcaagtct
    23101 ggacattttt gatcaggatg acgtgctggc ggcaaaccgt gtgctggctg ggcgtctcga
    23161 cgccgccgcc gcaccgctgc gtgagcatcc gcgcgtgatg catttccgtc gcaccggcat
    23221 gatctgggcg tttgatgtgc gcgaggcccc ggccggattt tcccgccgca tggcccaggc
    23281 tgcgctggat cgcggtgtgt tgttgcggcc gatcggcgat accgtatatt tcatgccgcc
    23341 ctatatcatc ggcgaagagg agatcatctg gcttgtgcac ggtacgctgg ctgctctgga
    23401 tgtaaccctg gcaggttgaa gtgctgtccc ctctccggga ggttgcagtg attgcgccat
    23461 aatcgtggca ttttgctgac gtaatagacg ttgttcccca aaaaaagcgt acaatttttc
    23521 cggtgtgctc ttcttcgtca tgacttggga ggcagcgtat gttctggcaa aagcgtttcg
    23581 atgatttcgc cgagcggatg cggcggtatg acctgccgtt gcgacttgag ttgtggaatg
    23641 gccgggcgtt tgatctgggc gccattccgc gggtgacggt gcggctgaca tcggcgaccg
    23701 cattgcgcta tttcctgcca cccaccctcg acaaccttgg gcatgcctat gtggagggtg
    23761 atatcgatgt cgatggcgcg ctgccggaca ttcttgatgc ggcggtcggc ctggccgagc
    23821 atggcgggaa ataccaccgt catgcaccgc aaatcgtgcg ccatacgcgc aggctcgatg
    23881 ccgaggcaat ccactatcac tatgatgttt ccaacgagtt ctattcgcac tggctcgacg
    23941 acaacatggt gtactcctgc ggttatttcc gtaccgggca agacagtctg gaaactgccc
    24001 aactccagaa actcgatcat atcctgacca aactgcgtgt gcgccccggc gaccgtctgc
    24061 tggacatcgg ctgcggctgg ggagcgctgg tcatgcgtgc ggcagagaaa ttcggcgcgc
    24121 acgcggtggg catcaccctg tcgaccaacc agtatgaact ggcgaaggaa cgcattgccc
    24181 gcgcaggatt gtccgatcgc tgcgaagtgc gtgtcgagga ttaccgcgac gtcagcggca
    24241 gcttcgatcg catcagcagc gttggcatgt tcgagcatgt gggactgaag aatctgcgcg
    24301 gctatttcac gcgcatgcgt gaactgctgg ccgatgatgg ggtggttctg aaccacggca
    24361 tcacagcggc cgacccggat tcgctcgaaa cgccatttgg tggcggcaat tttatccagc
    24421 actatgtctt tccgcatggc gaactgccgc acatcggcct ggtattgcag gaaatggcag
    24481 cggccggact ggaagcggtg gacgtcgaga gcctgcgtcg ccattatgcg atgacgctgc
    24541 ggcactgggc tgcccggttc gaggcagccg gcagtgtgct cagggaactt gctggcgaac
    24601 ggcgctggcg catctggcgg atgtatcttg cggggtgtgc gcacgggttc gaacagcact
    24661 ggatgtccat ctatcaggtt caggccgtca aggcgggcga cgcggtgctg ccattgaccc
    24721 gcgattatct ctatgccacg tgaatgaccg ggggggtgag gcagtgcgca tcgcaggctg
    24781 ttatactgcg acaggtttcc ctctggtctg gcgtggacgg tgataattcc ctggcttgcg
    24841 attaccggca ttggccgctt tatggtgttg acaccagtgg cagtggtact ggccgtgtgg
    24901 ttcggcatgg cgcgcgactg gcgtcacctg tttctctggc tcggtctgta tgtcggtggc
    24961 atggcggtcg tggtcttgac caagattgcc tttatcggct ggggcctggg cgtcgaggca
    25021 tgggattaca ccggcatgag cggacatgcc atgcgcgcgg ccagcgtgct tccggcgctg
    25081 ttctggttgc tggcggctca acgcggacag gcggtgcgtg cgctggcgat cgtcgctggc
    25141 gcattgtgcg cgggagtgat ctgttggtcg cgcattgtgg tgcatgcgca ttcgatgtcg
    25201 gaagtggtgc tgggtggcgg gtttggtttc gcattgttcg ccgtgttgca ccattcgctg
    25261 agcagtccgg gggcgcgccc gccggtgacc tatcgctgga tgctgctggt cggtgcattc
    25321 agcatgctgg gcacgccagc cattcagccg ttaccgacgc agcagtggat cacgcgtgtc
    25381 gcattgctgg tgtccgggca tgcgcatcct tatacccgtc agggttggca ccggcaccat
    25441 ccggttgtcc agcccatgcc atcggactga ttgaccacgc catcatgccg tcatcgtcca
    25501 gggcatgcgc agcgtcttga gacgcaagaa cggccgttcc accagatacc agctggccag
    25561 accgcatgcc acggcaagca cgaaggtttg cattaccagt gcggtggcgc tggtgtccgg
    25621 tcgggccgcg agcagcagtt tgccgacagg ccaggcatac agatacaccc catacgaaat
    25681 atcaggcagc cgattgtagg ccagaagccg tccgcatttc gcaccagcca tgccgaacag
    25741 caggtaaccg aagaacagtg cgtcgccgag ttcgacgaaa cggcgtgagc acatcgtcag
    25801 gatgaacagt gcgagcgcga tcagcgcgcc ctggcggctg aaagaaatgt ggtcgcggaa
    25861 caggtaatag ctgccgccga aaaagaaaaa agtgaaaaac gacaggtaat gcggccccag
    25921 cgggtggaat gccgtcagcc cggctgcgtt ggtcaggtcg acaagcgcaa tggccgccgt
    25981 cgttgcgccc caggctgttt tcatgcgtgc accggacgtc agtccgaatg ccagcaccag
    26041 cagataacag atgaattcgt aaggaatggt ccacatcgac gcattgatga aggggtggtg
    26101 gctgccctgg aacaccggtg gaatggccgg ttcgtcgagc cgcagcatgc cggcaagaaa
    26161 tgtcggccag cggaaatgca gccagtagtc tgcattgcct gcaaacggcc cgatgagcag
    26221 tgcacagacc agcgacgcgg cgataaatcc gggatagatg cgcaggatgc gttttttcag
    26281 aaacgacccg gcaagcggtg acagttgcca gctctgcacg atcaggtagc cgctcagcag
    26341 gaaaaagccg ttcacggcaa aatcaccgaa tgacagtgtg tggaacaggc gcgtgagcag
    26401 ttcgtgcgac cggtcaccat agatgatttc cgggctgtgc gacacgatga ccagtgtggc
    26461 gagcaccatg cgtatcaggt tgaaatgatt gtcgtgccgt gcaccgggtg acggcgtggg
    26521 cagccgttcc tcgccggtgt ggatcatgag tgctgttctc cgcgctgcag gcgcgccagt
    26581 tcgtgcgcag gcatggcgct ggcgatgaac gacgggttga gtacctcgtc cttgccatag
    26641 cgcagtgcgc tgccatcagg ctgaaacaca tggccgccgg cggcttcgag caccgcctgg
    26701 gctgctgcgg tatcccattc gcaggtcggc gccagccgtg gatagacgtc ggcggcgcct
    26761 tcggccacac ggcagaactt gagtgaactg cctgcttgca ccagttcgtg catgcccagc
    26821 cgcgcgatga aatcgcgagt ggcatcgttg aggtggcttt tgctggccac cacacgtacc
    26881 ggcgcgtgcg gggctggcgc agcagcgacg tgaatcggct cacgccggtt gccggattcg
    26941 cgccatgcgc cgacttcgtt cccgccccag taggcgactt tgagcgctgg ggcgaacacg
    27001 atgccgaata ccggtcttcc ggcattgatc agcgcgatgt tgacggtgaa ttcaccgttg
    27061 cgggcgataa actccttggt gccgtcaagc ggatcgatga gccagaattg tgttggtcgt
    27121 gcgcggtgcg tcagcgcctc gtcttcttcg gacaccaccg gcagaccggg ggcgagtgcg
    27181 cgcagtccgt cgacgagaat gtggtgtgcg gccagatccg catgggtcag cggactgtta
    27241 tcggccttgt gttcgaccgg tgcgggttgc gcctgcgcgt agatttccat gatggcgtgc
    27301 ccggcttccc tggccagtgc gagcacggtc ggaatgagca aaggatcgat tgcggattgt
    27361 cgcattttgc tgtagattct tgttaattgg aattgacatg attatacatg ccaatgttac
    27421 agaaatttca aacagcattg caaattacga cacgggcctg atgacatgag cgtgcctgaa
    27481 ctgcttggac ttgatgcacc gcaaccctgg gatacggagc atgcgttccg gcatttgcag
    27541 caactggggc gcgccgaatc gaaacggacc gccgagcggc tattgcatac actgggcatg
    27601 ctgccggccg agcttgctgc cgagttgctg cgtgacgatc gggatcgtcc gccggtaccg
    27661 gcgatcctgt cgtgggagat cgagcgcgcg cgtgcgcgcc gcacgctggt gttgttcgtc
    27721 cagttgcacc ggttgccagg tggcggcgaa tgcctgcacg ccaatgacag ccgcggtacg
    27781 cgctactggg tgccgctgga cgaagatgtg ctctcgtctg cagcgctgca gcatgcgttg
    27841 cagacgctgc acgcgcatat cggcaagccg ctggcgatct ttccgcacgg acgtatcaca
    27901 gcgttgctgc gcgagctgcc g
//
LOCUS       DAIANL010000114         7958 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3210775, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000114 DAIANL010000000
VERSION     DAIANL010000114.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3210775"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1674 a         2617 c         2159 g         1508 t
ORIGIN      
        1 cgcaaccgcc aggtggggct caggctggcg accggcgcca gcctgacaca cattctcgac
       61 gaactgggtc atgtggccga gggcgtacac accgcgcacg aagtcgcgcg cctggcccgc
      121 acccttgatg tggaaatgcc catcgtcacc gcagtcgacc aggtgctgca cgaacgactg
      181 cctgcgcgca aggcggtcga cgcactgctc aaccgcgaac cacgtgccga gtggcaccac
      241 tgaaccaacc gcagcatccg caaactcata ccgctgtcag atgcgcaatt tccatggcca
      301 ccgtggcctt ggcctgattc atgtccggac agcgtccggt tccgtacaga cagcaggcaa
      361 tctggttcag catcggcatc ggcagcgcat ggtgtccggc cagactttca cgaatccagt
      421 cagcaatggc ggtcacggac tggccgaccg gcaagtgcag cggaacctgc tcgctaccga
      481 cttcagcttc gaacagcatc tggcacaccc cccggctgca atgccacaat ctgggtcgcc
      541 ggtggctgtt ggcaatcgcc tcaccctcgg taccgaccag cagcagggca tgtgccgacg
      601 aatctgccag tcggcggcgc atgcgatcga gctgagcctc gtcctgcact ccaatcacgc
      661 gcagggcatc cccgcgaaac ggtgccagca acttgaccag ttcgtgcgcc gtgccacgca
      721 ccccgatccg cgcgtgctgt gccagcaggt gtgccagccc cggcgcgatc accgctgtcg
      781 gcacgaacgc cagcccgtca cgggacaacg cctcattggc ctgcgtcagg ctggcacacg
      841 gcagaatgcc cagttcgcgc aacacatgcg ccgtagtcat gcgaccctgg ctttccagac
      901 tgccatgcac cagtaccggg atgccgaact tcaccagcag cagcacaaac aggggtacca
      961 ggttaaattg atcgcgcgtg ccacaatagc tgggaatgac aagcggccgg gttgccccgg
     1021 acaccggcca gcgcagaaaa tgctggcgcg cctccagtgc acgcacgaag ccggcccact
     1081 cgctgtcagt ctccccgcgt acagcaagcg ccatgagtgt tgcgcccatt tccatttccg
     1141 gtacgccgcc gtcaagcagt gcgccaaaca attgcccggc gtccccggtc cccaggccgt
     1201 ccgcttcgtg caccagatga cgcaacaacg tcgcatagct catgatggcc tcgcgcatcc
     1261 gtgcctgtgc cgcagctgaa cgtgcgcatt gtcagaagtg tgtgttctgt gtcccatgta
     1321 cggtcatgaa gcgataaccg taccatcgcc gccattccgg cattttttgc ccgaaacggg
     1381 gcgagacgca ccagtcgtgt gcggcacgcc tgcccggcgc accgaagctg ctcatacgcc
     1441 ggaggcgtca gttggcagag tagccgggaa gacctgacgg gggaggatag cctgctccgg
     1501 caggtggata ggccggaccg ggcggggggt agccgccagc agcggcaacg ggcgcgacat
     1561 gtaccccgac ttcgatgaaa cgaccaggct gctgtggcag caccatgttg agcatatggc
     1621 cgcgataacg ataagtgaca ttatatccgg ccagcacctg ctggtagtgg ttgcgcattt
     1681 cgcaatgctg gaccggctgc ggcggctgct gcaggcccgc atccacacgg tcacccacca
     1741 gcgcgccggt tacggcaccc acggcggtgg ctgcgctgcg gccgtttccc tgaccgaccg
     1801 tatttcccag caacccgcac gcgagcgcac ccaccagtgc gccggcaatg ttgtgatccg
     1861 tcccggccga actgtcgctg gccacggggg catcggtcca gcacacctga cgcggctgat
     1921 ttacctgacg gtacatcggt gttgccgaca gcacttctgc ccggtcggta aaataacccg
     1981 cattcatcct gtcgtccgca ccggcaccct gcgcggccac cagcgacagc gccgccagcg
     2041 gaatccatcg actgcatttc atgattttct ccatacacgc atcctgtcat tgttttccgg
     2101 cttccggccg gacgatcaac ttctggccac cgcctgtcag ttctgccctg attcgtgccg
     2161 ttgccggcgc tcgaaaccga aaccaaaacc ctcctgcgcc ggcgagcgca ccccacccat
     2221 ggtcggaagt ggccgggcct ggggctgcat tggtggttgc ccggacatgc gtacctggct
     2281 gacaccatgc atcatgttct cctgctgcgc ccgccgccag tcatgccgca tgcgaggcgg
     2341 ttcatcggcc ctggcaactc ccagactggc tgccagcaac acccctgtca tccatatcac
     2401 atgccgaacc ataacaatcc actcccaccc tgccccgcca ggggcaccgc cacccatcgg
     2461 acacccacat ccgtcgcctt gttcacgccg tacgccgcat cgacacacat caccgccgac
     2521 cgcccgcact gcatactgcc gatagtatcc acctgtcgct ttttaccaga cgtcatgatg
     2581 gaggacaaac gcaacacaat catttcggcg tcacgatgtt ctgttacaaa atgtaaccgc
     2641 aagcccgtca ccggtggcat cgacgaactg catgccagct tgcgctatca tactgtctga
     2701 tcatgaacgt atttttgctt gccctgttgc tgctgctcgc ccaactggcc caggccgcca
     2761 gcctgcccga tctgggcgac gcttcgcagg ccggactgtc gcctgccgac gaagcgcgtc
     2821 tgggcagcga aatcatgcaa caaatacgcc agcagcccga ttactacgat gacccggaac
     2881 tgaccgacta tctcgaatcg atcggctacc gactggtcgc caactgcgac gaaaacaatc
     2941 agaatttcca ttttttcgcc atccgcgaca ggacactcaa cgccttttcc ttacccgggg
     3001 gctatgtggc cgtaaatacc gggctgatcg aggccacgca gaacgaatcc gaactggctg
     3061 gcgttctggg gcacgaaatc tctcatgtca cccagcacca catggcgcgc atgatcgagc
     3121 aacaacacaa aaatttactt ccgtccctcg gcatgctggc gctgtcgatt ctcgccgcac
     3181 gttccaatcc caacgttgcc ggcggcgcac tggcggcaag ccagggggtg atgctgcaga
     3241 accagctcaa tttcagccgc aacaatgagc gcgaagccga ccggcttggc attgaaacca
     3301 tgtctcgcgc cggtttcgat ccacgcggca tggtcagctt cttccagcgc ctgcaacact
     3361 atgaccggct cgacgaaaac cgctcccagg cctatctgcg cactcatccg ctcaccagcg
     3421 aacgcatcgc cgacatgcaa aaccgggtag cgcagatgcc ggcacgcgca gtcggtaaca
     3481 gcctcgaata cgacttgctg cgtgtcaagc tgcgcgtgct tgcgctgcca ccttcggtag
     3541 cgatcgcagt ccagcaccgc gaaatcaaca atcatcttta ttccagcctg ctggccgaac
     3601 actatggcct cgcgctggcc ctgttgcgca accaccagta cagcgcggct gaaaacgaat
     3661 acctcaccct ggcgcaatac caccagaaca gtcccatcct cgacatactt ggggccgaga
     3721 tccaccagtc ggcgggcgat ttcgacggag cactcgtgcg ttaccagacc gcatggcagc
     3781 actatccgaa ctaccggccg ctggcgtatc gttacagcga agcattgctc aaccatggtc
     3841 agccacaggc agccctggac atcatcgatg ccgaactgga gcagtacccc cacgatcatg
     3901 cgctgcatga actgcagtca cgctgctatg ccgcgcaaca caaggatttt ctgcgtcatt
     3961 atgcgcaggc acacgcgctg cacgaactgg atcagaccga tcaggccatc gagcaattgc
     4021 agctggcact caagacccac gatggtgatt tttaccagat gtcttcggca gaagccctgc
     4081 tcgaacgctg gcagatcgag aagaaaaaag ccgcctccgg ccacaacacc cactgatgtg
     4141 tcatgaccgt ggcggctgaa gggcgtgtcc tcagaacgcc agcacggcgt agatatccac
     4201 ctggttacct atggcatcca ccgtggtttc agccagcgtg agcggtgcgc tgcccggctt
     4261 gccgattgcg gtcggattgg gaatcgtcac gcgatggtct tcatagtcga ccaccagccg
     4321 cgtaaacggg gtgaaatggt actcgactgc cgccgctgcc gtctggaaca ggcgttcctg
     4381 cgccgcaagg ttgggcaagc ggtcataagc atcatagcgc agcacggctt ccacgtgatc
     4441 cgtaacgaag taaccgagcg atccggtata gccgtaggca cggttctcgc tgccagggta
     4501 gaacgtggca tcatactggg ctggtgccac gccgggcacc tcgttataca ctgccggcgc
     4561 ttcgatattg ccggtaccgg aataaaattc aagcttcatg ctgcgagcgc cctgcttcag
     4621 atagccgtca cgcgccgcca cgccgaaacc gtcacgtacc atggtattgg agacgccgtt
     4681 cagctcgggt cgcgcctgct gataccagac gaacccggtg atatcgttac ggaagatgcg
     4741 tgcgggtttt tccggcccga gcaaatagga aaactgcaga cgagccgctg caatcggacc
     4801 gttttgcgtg ctggactcga actgacgacc atactcgccc agcatcgcgc catatgccac
     4861 ctcggtatgc ggcgccacca tgaaccagtt ttccagttgc acgccagggt aacggaagcc
     4921 attattgccc gacagatcgg tacccggcac cagatagccg ttgtgcaccg tgctggcata
     4981 ttgtacgttg cggctataaa acaccggctg catcaactgg gtaattcccg tcggaaatga
     5041 gtcgaggaaa ttgaagtcca tgtaaccttc catcgccgtt tccgcgctcg gggcacgaat
     5101 gatgccgatt tcaaaccggt tgccgcccgg cagtttgttg ctgacggtaa tgttggcgtc
     5161 gatcagtttc gggccataat tgccgaatga gtatgtatat gcattctcgc ccaactcact
     5221 gccgacatag taggaaacgt ccggattcat gctgccacgc acgaacacac gtgcgcgctg
     5281 cacataaaga tccgtcgaat cctgcgcagt cggtcctacc agatcgggtt tagggatctg
     5341 atggttggat gcaggtgatg cctgaatatc ggtaaaaacc ggctggatga aaccaagcag
     5401 cttgccataa ccccattcag gcaaggcaat tgtttgtatg tcgaaccagt tatcggccca
     5461 ggcactggaa ctggcgcacg cgccagccca gaccatgacc gcggcatgga ccaggtgtga
     5521 tttcttcagc atgaatcgat gtctcctcgt gtagttatca acctcccact ccaggcctat
     5581 ggcctggaga tgaacggttg tgtatcggga tcagggctgt cccccgtccc gttttttccg
     5641 gccggtgacc ggaaatgacc acgcctggta cttcacgccg cgtgatcgat tccgcaccag
     5701 cctgcatagc agatattccg cttgcgcgag atatcgctgc gcatttttac gccatgtcgt
     5761 catgacatgc gcttattgtt ttattgaaat tattaaaatc taactatttt ataggttatt
     5821 aaggcgcgcg acgggccggc cttgcccccg atcaaccggg tgctctacac ttgcaccata
     5881 aggaccggca tattgccgtc cgttgcccca tcaccggagg atgcatgcgc atcaccaggc
     5941 gttccctgct caaacttctc gcaaccctcg ccaactggcc gttcgtgacc tttgccgcgc
     6001 agacaacagc tgctgcagcc ttcggcgaac acaacgccac cgatgcgtta cgcgacctga
     6061 atgccagccg tgccgccgcc agcactgata tcgtgctgcg cattccggcc aatgccgaag
     6121 accgcctggt cgtcccgctc gagatcacca gcgatcttcc ggacactcag gccatcgaca
     6181 tcctcgtcga acagaacccc tggccgctgg cggcgacatt ccgtttctcc aacggcgcac
     6241 tgcccttcat tgccacccat ctgcgcatca gtcaggacac tgatgtaatt gccatcgcac
     6301 actgcacaga cggcacctac accacgcaca aggccgttca tctcaccagc ggaggctgcg
     6361 ccagctaacc ggggctgcgc agaaaacgaa tggccatcca cagcatgcac atccgcgccg
     6421 tagcacaaaa cggcatcgcc gacatcaagg tgctcatcca gcatccaatg gacaccggtt
     6481 tactcaaaga cgcaaaaacc gggcaattta ttccagcgca tttcatccga cagcttgttg
     6541 caagcattca gggcaagacc gtactcgagg ccgagtgggg tcccggcatc tcgcgcaacc
     6601 catatctgaa cttccgggtc gccaacgccc atcccggtga cagggtggaa attcgctgga
     6661 cagacaacct tggcgacagc gacacagcgc acaccgtcat cgaatccgct gaacaatcac
     6721 ccgactgaac cccacttttt tcgcaaaccg accgctacgt gcgcacgcgg cgacagtttc
     6781 gcagaccaag cgcgtgcaag cggatgtaat tccgcacaaa tcaggctaaa atgcaggctg
     6841 attcttgtca gtattgcgta cgaaacccgt catgaacagc agcgaaatcc gtcagcgctt
     6901 tctcgatttc ttctcagccc acggccatac ggtcgtgcca tccagcgcgc tggttccggg
     6961 caacgacccc actttgctgt ttaccaatgc gggcatggtg caattcaagg acgtgtttct
     7021 cggccgcgaa tcgcggccct atgtgcgcgc cgtcagctcg cagcgctgcg ttcgcgccgg
     7081 cggcaaacac aatgacctgg aaaacgtcgg ttacaccgcg cgtcaccaca ccttttttga
     7141 aatgctgggc aacttcagct tcggcgatta tttcaaggcc aatgccatcc ggtttgcctg
     7201 ggatttcctc acccaggagc tccggttgcc gcaggacaag ctgtgggtca cggtctatca
     7261 caccgacgac gaggcctggc ggctgtggac cgaagacatc ggcgtgcctg ccgcgcgtgt
     7321 catccgcatc ggcgacaagc cgggtggcgg gtcggacaat ttctggcaga tgggggaaac
     7381 cgggccgtgc gggccatgca cagaaatctt ctacgatcac ggcgaggaag tcgcgggcgg
     7441 accgcccggt tctcccgacg aggatggcga ccgctatatc gaaatctgga acctggtatt
     7501 catgcagttc aaccgcgacg aatcggggac actgcacccg ttgcccaagc cgtcggtcga
     7561 cacaggcatg gggctcgaac gcgtctcggc cgtgctgcaa catgtgcaca gcaattacga
     7621 aatcgacctg tttgcggcgc tgattcgtgc cgcagcccga gtcacaggct gtactgacct
     7681 gggcaacaat tcactcaagg tcatcgccga ccatatccgc gcctgcgcct tcctcatcgt
     7741 cgacggcgtg ctgcccggca acgagggccg cggctacgtg ctgcgccgca tcattcgccg
     7801 cgccatccgt catggttaca aactcggcca gcagcagccg tttttccacc tcctggtgac
     7861 cgatctcgtc ggacagatgg gcgtcgctta ccccgaactg gaacaggcac gaccacgtgt
     7921 cgaacaggta ctgcgtcagg aagaagaacg cttcgccg
//
LOCUS       DAIANL010000115         2773 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_147790, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000115 DAIANL010000000
VERSION     DAIANL010000115.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147790"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          516 a          740 c          937 g          580 t
ORIGIN      
        1 aacatggagg tcatcatgac catgaggaac gcggcaaagg tgatgccgac cagcagggcg
       61 gcaaactttc ccctgtcgtt gaccagcagc ttgaagccga ttttcatgat gccgttgatg
      121 atgatgctcc cgtcaatcgc gcatcgttcc gcctgaaagt gcggtcgcgg ttcaggatgc
      181 ggtcaggttc attataggag attcagtcat gcctggctgg cacatcgggt caaccgaacg
      241 ttgtatttcc ggccagaagg ctgtacggcc ggcacgtcat tctcgtgtat catgagattc
      301 gctgcagggc gcaacaaggc tcgcggggtg tgggaggttc ggtccggtga tgacttcgtc
      361 tggagcggat gatgcataca cataattctc aaaacagact gtttgccgcg ctgctgctgg
      421 tgctgttggc gttccttgtc ggcgtggtcg tggccttcag cgaatttacg gcgcattacg
      481 aacaaaaagc ctcgaacaat ctgcaagcgg tcgccctggc acagcgcagc cggattgagg
      541 cttttctgaa agagcgacgg gctgatgcgg aagtggtcgc gttgcgtgcg gctggatcgc
      601 tggcgttcgc ttctccccgg aagcgtgacc ggggaccccg ggcgaacgaa cccctggcgg
      661 gtttcatcgg ccaggtggcc aaggcttacg gctattccaa tatcgtggtg atggaccgga
      721 acttgcaccc tctggctgct tgtcatgaga cgcccgtcga tgcggcagtg ctggatgcaa
      781 tcaggcaggt gcgggtaaca gggcggcctg ttctggtgga cgtgttccgc gcggccgatg
      841 gcaaacagta ttttggcgtg gcaagcccga tagccggtgc gggtggagtg gcaccggcgg
      901 tggcggggat cgtctatttg cagatggatg cggatgcgct gtacggcctg acgtccacat
      961 ggccggaacc catgacaagc gctgaaacgc tgttcgtgga acagcgtcgg ggacacgtgg
     1021 tgtacctgag tccgctcaga ttcgctcgtt ctgcgcctgc tgtgggtcgg tggctgacag
     1081 cccggctgga tgccggcaat ccggctgcgg gcccggtgtc gggtacggat tatcgcggtg
     1141 tcgatgtgct gggggtgctg gtacccatcg ggggtacgcg ctggtttctg ctggtgaaga
     1201 tggaccgcgc agaagtcgtt cgtccggtaa tgctgttgcg cgatctggta tttggcgggg
     1261 tagggttggt gctactgttg ctggtgctgc tggtttatct ggcatggcgc cggcagcagg
     1321 ccgaatccat ggcggccgac gcgcgcaagg atgcacgtta caatgcggcc cggaaagcat
     1381 cgatcgacgc ctacgtggtt tacgatgcgg ccgggcgcat catcgaagtc aacgatgcga
     1441 tgacgcggat gaccggttac ctgcgcgagg aattgctggg ccggatgctg acattgctgc
     1501 atgtcggctg gaacgatgaa caggtgcgcc agtcgattgc gcgcattcgt gaagcgcagg
     1561 gcgaccggca acgcagtcag tggcgcagga aaaacggcga gctgctggaa gtcgagatca
     1621 gcagcagcta tctggccgat gcggcgactg aaacctttca ttcctttgtg catgacatcg
     1681 ggccgcagtt gcaggcgcag cagcgtatcg agcgcctgaa catgttgtat cgtttcctca
     1741 accacgcgaa cacggccatt ttcagccagc atacccccga ggccattctg acgacctttt
     1801 gtgagggtgc agtgcgggat ggcgggttca ttctggcctg ggcggggctg ctggatgagg
     1861 cggccgggct ggtcaggccg gttgccgcat cgggggtggc cgtggattat atccgccagc
     1921 tggtcatcac caccaacccg gcgttggcta ccagccacgg accgacacgg atgtgcatgg
     1981 ttcagaaaca aatcatcagc tcggacgatt ttcagcatga ccccgccacc gcgccctggt
     2041 acgagatcgc ccaggcgcat ggcatccatg cttctgcggc cgtgccagtg ctggtggacg
     2101 gcaaggccgt ggccgcgctc acattctact cggacaggaa ggggtatttc agcgaagaca
     2161 tccgcctgct gctggaagag gccgtgcgta acgtctcact ggcttttcag gcggctgagt
     2221 cggaacgccg caaggaacat gccgaagccg agcggcaggt gagcgagaac ctgttccggc
     2281 gggcatttga tgcttcgccg gtgccgatgt tcatcgagtc gatttcgaac agaacgttgc
     2341 gttatgtcaa tccggcgctt acgcggtcgt tggggtacga actggcggat gtgcctgacg
     2401 agacggcctg gttccggcag gtgtatcccg acgccgcgat gcgtcagacc ctgtggcagc
     2461 aatggaatca gaacgatctg cccgaggcgg ctgcgggtgg tgcagggcat gttgtccagt
     2521 cgccggaagt tctgctgcat tgccgcgatg gcagcagccg aacctttcgc ggcatcatga
     2581 ccgcaattgg cgatgatgcc attgtgcagt ggctggatct gaccgagatc aaacatgccg
     2641 aggccgaact ggtggaaaac gaacggcgtt tccgcggcat gatcgagcag tcgctgatcg
     2701 gaatctacgt cacccaggac agccgtatcg tgtatgtcaa tccacggttg tgcgagatcc
     2761 tgggatggaa atg
//
LOCUS       DAIANL010000116         3734 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_759630, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000116 DAIANL010000000
VERSION     DAIANL010000116.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759630"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          705 a         1195 c         1093 g          741 t
ORIGIN      
        1 gccgagggta tcaagctgca aattgccagt ggtggaggca atgctgccct gggtgttgtc
       61 cagctgggcg ctgatgatgc tcaatgctcc gctcgcagca atgacgccac cggcggtatt
      121 actgatctgc ccggccgtca atgtggcacc gccggcacca cccaacaggg caccgccagt
      181 attgttcagg ctggcagatc ccgcattcag aaccagctgc cccgccccgg aaagggtgcc
      241 cgactggttg ctgatgcctc ccagggccgt caccgtggtc acaagattac tggccatctg
      301 gccatggtcg ttgaccagtt gtccggcagt atttacgccc agaccacccg tggcatcgat
      361 ggtggcacca gtattgttga tggatgcgcc gttcaggctg gcattgccag tcacctgcca
      421 gttaccattc gtgtttgaca gagcgccagt agcggttgcc accagatttc cgccgctctg
      481 gatcgacacc ccgcttgcgg acaggttgcc ctgcgtggca atcaggttcg ccgcaccacc
      541 tgactggatc gaggttccgt tcagcgcaag attctgcgcc tgcagggcca gtgtggtgcc
      601 tgcgctgatc tgccctgccg tatcattgat cgatgcggca ccgatctgca ttgcgccagt
      661 gttgtagaac aaaccggtgt tcgtcagcgc gccctgtatg gcaaagtttg caaggttgct
      721 gccatatcca tcgatggcac cggaattgtc gaggctcgcc gcctgcaggg cgagcccgtt
      781 gccggcatcg atctggccgg tattggtcat cgcccctgtg ctggcgatca gcatggcact
      841 accgctgtga gtcgcgccgt tcaatgtggt attgccagcg gtattcagag taagcgcctg
      901 ccgggcataa gtatcgccca gcgtcagatc gccattggcg ctcagcgtca gattgccctg
      961 ggatgcctgc aatcgcccag tgctgttgac gcccagcccc tgttcggtgg cgatcatgta
     1021 gatctggttc gcatacatgc cgcccaggtt tttgatgtcg acggcaaaca gcggggtact
     1081 gcccatcgcg gtctgccgca cggcctgcaa ggtgccatag tcgatctggt tggcaccggt
     1141 gactacgttc aggttttgcg cccagatatt gccgccgtcc acgacgagat tgcgcgccaa
     1201 gagatcgagc tgcgccacgc ccgaggcatc cagcccctgg ctgccgacgc tgatctggcc
     1261 gccctgcacc agaaatcctt gcaatccgcc ggaagcattg aactgcggca cgccggtcgt
     1321 cagcgtgacg cggttggtgt tcaaaaaccc gcagccgtca caggtgatgc cgtccggatt
     1381 cgccaccacg atgcccgccc gctgcccggc cacttccatg gctccgagca gttgcgtcgg
     1441 gttaccgccc acaacctgat tcaggatgag ggtggccggt gtggtcgcca gcagggggtt
     1501 gccggagatc caacccccca actgcgtact ggtgttgccg gtactgttgt tcaggatcag
     1561 cccgttactg ccgacgttga actgactgta cagattattg gaaacgccgc ttgcattcgg
     1621 tgcggcaatg tcgacgatgg gtacgccatt gcgcgccgtg tcggtcagtg gacgtgatgt
     1681 cgccggtgca ttcggtgcca cgacaatgcc gccggcatag gcaggcagca ccgccagcac
     1741 agtctgcgcg aggtatgtgg ccagggtcag gcgaaccatg tggcgttgcc agcgccgggt
     1801 gggcggcgtg ctcatgacca gctgcggcag ggtgaacgga tcttgcacct gtgttctacg
     1861 acgccctggc accccatgca acatcgaccg cctccgtatc agaataatcg tgtcaccgac
     1921 agataagcat tgagccgccc agaatcgaac ccggccggat gatacagagg cgtggccagt
     1981 gccagatcgt aacgtgtcga catccattgc ccacgcaccc cgaccgccac acccgcaagc
     2041 tcggtgccga ccagatttgc agcgctcggc ccccacaccc gcccggcatc gaccgcggca
     2101 taaaccgact gctgcccatc tcccaaccag ttgaccggat gtgccagctc gttgcgccag
     2161 gaatagccgc tgtcggccat cagcacggta ttgccgtcga aaccgcgcac gctgtagcgg
     2221 tcaccgatgt acatctggtc ttcgctgatc agcgtgtcgc gcgtggcctg tccgtgcagg
     2281 gtactgctga acagcaacgg cgtactcgcc acggaaaacg gctggttcca gctggcagtc
     2341 aatccccaga tatccgggcg caccgtcagt ccgccttgcg gcgccgtctg cagatcattc
     2401 tgcgcgccgc cactggccag tcccatgcga taatccagcc ggacatcgac ttcaccgttc
     2461 gatggcagct tgaccgtact gctgatgcca cggtcgaaat tgacggtatt caactgctgt
     2521 acaacaactt cgacattgtt cagataattg gacgcctgtt cggtgaacaa tgccgcaaaa
     2581 accccgagtt ttgccgacgc actgcgccac agtgtccagt cccattgtgc ccgtgcgatg
     2641 cgggtgtccc cgctggacag aaactgcgcg gtcgtcccct gcacgatctg cgcgtagcgc
     2701 acatagctgt cgctgagggt gaacaggttg tagtcccacg gaatgctgta actcaacccc
     2761 aggctctggc tacggtggct gcccgtgacg tcctcgacgt tgctgctggc attcaggctc
     2821 agaatatcgt taaggccact cgggtcatcc agcaccatga gcgccgacgc catgggccgc
     2881 ccgacatatt cgctgccgga attgtcgagg gtcacgccgc catgcaggcg gttgcccaaa
     2941 cgcttgcgtt ggatcaccac cacactggta tcgggttcgg caccgggttc aagcaatgtg
     3001 tgcacgctct ggctggacaa ggcatccatg ctctcgatcc cctgttccag atcgcgcaca
     3061 tcgagcacat cgccaggaga tgccggaaac gcattacgcc aggtgcccca ttcatgatcc
     3121 ggcgcaccgg tatccgcccg caccatgcgc acagccgaca ctgtgccaag atccagatga
     3181 atgtgcaaaa tcccgcccga cagcttctgc ggcgggaaag ccgcacggct cgtggcatac
     3241 cctcgggcca gcagttcgtc gttcagcgtc cgggaaagga catccagccc gcgcgcaccc
     3301 acgcactggc cgatgaccgg ctgcacgaag gcatcgagcc agtacagctg gctggcatga
     3361 gcaccttcga aatggatggc atggatgaca aaacagggcg attccaccgg cagacgcgcc
     3421 agagagggcg tctgcccagc cgacgcctgc aactggttgc cggatgctgg ctgcatggtc
     3481 agggtttgcg ccgcacgcgt ctgctgttgc agcagtccct gcgcttcggg ttcgggcaag
     3541 gttgccgccc gcgctggtgc cgccactccc aggcagacgg ccagaatcca gcctgtacac
     3601 cggctttgca cacacatccg gcgccatgct cctctcatga acggatccag tattgttgtg
     3661 attaattgtt agccgcgcat gctaacaaat ccttatcacc ctagcaatgg ctaaggcggt
     3721 ataacgatat ttat
//
LOCUS       DAIANL010000117         8704 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_149959, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000117 DAIANL010000000
VERSION     DAIANL010000117.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149959"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1831 a         2366 c         2685 g         1822 t
ORIGIN      
        1 acagggcgga cagcagttgg tccacacgcc gcaccgggca ccgcaccacc agtttggcgc
       61 ggtggccaag aatcagcgcg ccagcaccgt catccgcccc ctggatggcg tgaatgcctg
      121 ccagcggctc atcctcgagc cagggcacgt gttcgcgcaa agcctgccac aacagccatc
      181 cgtggtcgga tgcaaccatc cggccacgca gcgacaggtg cacatcgctg caaggttccg
      241 cactgttggc gtccgttgca gcgggttcgt cgtcaaacca gcttattttg tttctccctg
      301 agcgtcctgg ctatcctggt cagcatgatc ggcccagtca agcagggctt tacccaacgc
      361 acgcgccgta tgcgcgtcga tgtcgaagat ggtaaagcgc ttgatctcgc gtatgcgtac
      421 acgcagagtc acataatccg cgcggtgcaa cacatgctgc agttcgattt cctgccggta
      481 gggtgtggca agaatcttgt gcagtgagcg gatttcaatc tcgtccaaga tgcatattcc
      541 tcagaatttc agcccaaatg gctgtcatgt cagtgacatg aagtctgtca ttatcgtcac
      601 cggaagaaac ttacccgaaa gagtgggtgt ttgtcctacc cgtttgggta ggttatttca
      661 tacccattca gcttatggaa ttgatgattt aggtgcatgt aagatgagtg caacgaattt
      721 gacatcggaa tgacaaacgg tcccgtttcc cgggcataag gtcttttgcg tcagggaagc
      781 ggatttgtca ttttatgggc cgctcgctgg ggtgcccgga acgattcgga tgacgccgat
      841 ggcggatggt gctgggtgct ggcagcattc aaatgacgaa tgatctggca aatgccggtg
      901 gaatttgagg agtggatatg accaagaaaa tgtatgacac gccgttgctc gatcaactgg
      961 aaagtggccc gtggcccagt ttcgtgacgg gtctgaagcg tctggccaaa gacaacgaca
     1021 tgatggtgga cctgatgggg cagcttgaac gttcctatca ggaccgcaag ggttactgga
     1081 agggtggcac ggttggggtg ttcggctacg gcggtggcgt gatcccgcgt ttcagcgaac
     1141 tcaagggcga caatggcgag gcgctgtatc ctgcggctgc cgagttccat acgctgcgca
     1201 tccagcctgc agctggtatg cactacgata ccgcaacgct gcgcaagatg tgcgatgtgt
     1261 gggaaaaata cggttccggt ctgatcgctt ttcacggtca gagcggcgac atcatgttcc
     1321 aggggatttc taccgaaaac gtgcagccgg cgttcgacga gctcaacgaa atgggcttcg
     1381 acctgggtgg tgccggcccc gcgctgcgga cgtccatgtc ctgcgtcggc gcagcccgct
     1441 gcgagatgtc gaactatgat gaggctcgtg cattgcgtac catcatcaat gccaacctcg
     1501 acgacatgca ccgtccggcg ctgccgtaca agttcaagtt caagttttcc ggctgcggca
     1561 acgactgtgt caactcgatc cagcgttccg acatggccgt gatcggcacc tggcgcgaca
     1621 acatccgcgt caatgaagcg ctggccaagg actacatggc tgcgcatggc atggaaaaac
     1681 tggtcaacga cgtgatcaac cgctgcccga ccaaggcgct gtcgttgaag aacaaagcca
     1741 acgtcgccac gggcgagagc atttccagcg tggcggtgac ggatacccag gcgcttgaaa
     1801 tcgacaaccg caactgcgtg cgctgcatgc actgcctgaa cgtcatgccg ggtgcgctgc
     1861 tgccgggcga agacaaaggc gcatcagtac tggtgggtgg caagagcgtg ctcaagatcg
     1921 gcgcgaccat gggtactgtg gtcattccgt tcatgaagct cgaatcggat gaagactatg
     1981 aagcgctggg cgagctggcc cgcaacatca tcgacttctt cgccgaaaac gcgctcgagc
     2041 atgagcgtac gggtgaaatg atcgaccgca tcggtctggt gaacttcctg gaaggcgttg
     2101 gtctcgaggt tgatccgaac atgattctcc agccgcgcag caacccctat gtccgtaccg
     2161 acggatggga cgaggaagtg gagaagttcg tcgagcgcaa gcgctcggcg ggctgatggg
     2221 tacaaacctg cactgaaaca agttgacctg gagagaacaa gttatggctg aattgcgtgc
     2281 gccaattgaa tgcggggtac cggatccgat gccgctgatg catccggtga tggcgaaaaa
     2341 ttatggcaaa tgggtgttcc acgatcaccc caaggccggt gtgctgcgcc accgcgcagc
     2401 gagcggcgac gagctgtgga ccgtcaaggc gggtacccag cggcagatgg acctgtatac
     2461 catccgcaag ctgtgtgata ttgccgacca gtatgctgat ggtcatgtgc gtttcaccgc
     2521 acgcagcaac atcgaataca tggtgtccga tgctgccagg gtcgaaccgc tgatccaggc
     2581 gctcaacagc aatggttttc cggtgggtgg caccgccaat tcggtgtcga tgattgctca
     2641 cacccagggc tggctgcact gcgacatccc gggtaccgat gcttcgggtg tggtcaaggc
     2701 gctgatggac gaactgtacg acgagttcac ccattgcgag atgcccaacc gcgtcaagct
     2761 gtccacatcc tgctgcgaaa tcaactgcgg cggtcaggca gacatcgcga tcgtggtgca
     2821 gcacaccaag ccgccgaaga tcaaccatga cctggtggcc aatgtgtgcg agcgtccgtc
     2881 tgtggtggcc cgttgtccgg tagcggccat ccgtccggca ctggtcgaag gcaagccttc
     2941 gctggaagtg gacgagaaga aatgcgtgtg ctgcggcgcc tgctatcctc cctgcccacc
     3001 catgcagatc aacgaccccg aatactccaa gattgcgatc tgggttggag gcaaaaactc
     3061 caacgcaaga tcccggccca ccttccacaa gttggtggcc tccggtttgc ccaacaacgc
     3121 accgcgttgg cccgaggttg ccgaggtggt caagaacatc ctctccgtct acaaggcaga
     3181 tggtcaacct tgggagcgta tttcggactg gatcgaccgc atcggttggc cccggttctt
     3241 tgaaaaaacc ggattgccgt tcaccaagta tcacatcgat gactggcgtg gttcgcgttc
     3301 taccctgaac gcctccgctc acatccggtt ctgacggaaa tctgcaacgt gtaggcagcg
     3361 cgcagcgggt ctgacccgca cgcgccagcc ttgccgaaat agaggggaat agggtatgaa
     3421 attcgggata ttggtcaacg aaggcccgta cacgcaccag gcagcagaca ctgcctacca
     3481 gttcgtgcag gcggcgctgg agaaaggtca tcaaatccac cggattttct tttatcacga
     3541 tggcgtcaac aacgccacca aactgaccga gcctcctcag gatgaccgca atgtgatcga
     3601 gcgctggagc aagctgggca aagagcatgg catcgacctg gtggtgtgcg tggcagctgc
     3661 actgcgtcgc ggtatcaacg agagcaatct ggccgagggt ttccgcattt ccgggctggg
     3721 tcagttggtc gagtcgggga tccaggcgga tcggctggta gtgtttggcg actgacggga
     3781 gtgaaatgaa gatgaatgaa acgacaggcg gaatcgacga cggagatgaa tcgggcgtgg
     3841 ttcgcaagtt catgttcgtc aaccgcaagg caccgtacgg cacgatctac gcgctggaag
     3901 gcctcgaggt ggtgttgatc agtgcagcgt tcgatcagga cgtcagcatg gcgttcgtcg
     3961 atgacggtgt gtaccagctc gtcaaggggc aacacaccaa ggcgatcggc atcaagaatt
     4021 tctcgccgac gtatcgcgcg ctggaggggt atgacatcga aaagttgtac gtcgatcagg
     4081 actcgctcga aagccgcggt ctgaccgagg ccgacctgct ggtgcctgtt gaagtgctgc
     4141 cgacgtccga aatggccgag atgatgcggt cgcaagacgt gctaatcagt ttttgaggga
     4201 gagtgtggat gctgcacatc gtcaataaat ccccgggcga aaacagtacg ctggcaagct
     4261 gtctgcgtat cgcgcgtggt ggcaaggatg tcctgctgct gaccgaagac ggggtctatg
     4321 ccgtcacggc cggaagcaag gctgctgaag aagttcgcgc tgccatgggc gacgtgcggg
     4381 tctatgcgct gtggccggat gtcgaggctc gcggcatgca agaccgtgtt ctggatggcg
     4441 tgaaaatggt cgactatgcc ggctttgtcg atttggtggt tgaatgtgac aacgttcaat
     4501 cgtggttgta actggttaac ggacaaatta aggaacggat catgcctgaa ctcgaagtga
     4561 atggaaaaac ctatcagacg gatgaagaag gttatctcat caatcttggc gactggaacg
     4621 aagacgtggc caaagtgctg gccgaaggcg agaaggtcga aatgagcgac aaccactggg
     4681 aagtggtcaa ttttctgcgt gaatattaca acgacttcca gatcgctccg gcagttcgtg
     4741 tgctgaccaa ggccattggc aagaagttcg gtccggacaa gggcaacagc aagtacctgt
     4801 acgagctgtt cccgtacggt ccggccaaac aggcgtgcaa gatcgctggc ctgcccaaac
     4861 cgaccggctg tatctgacgt cgtatctgta ccggtcggtc gactgactgg ccggtagggg
     4921 aagctggttc tgcggaatcg gcttcgattg gaattcggga cttgaaccag gcgctcgcat
     4981 gtcttcaatc accatatttt tcgcaattct gttctacctg gcgacgctgg tgctggtcgg
     5041 cggggttgcg tacaaaatct accgctacgc caacacaccg gcgccgctca aaattccaac
     5101 catgcccgcg ccgctcaccc gaacgggtgt tgcctatcgc ctggggcgcg aggtggtact
     5161 gtttgaaagc ctgttcaaat ccaataaatg gacttgggta ttcggctgga tgtttcactt
     5221 cagtctgctg ctcgtgctgc tcagacacct gcgctacttt atccatccgg tatgggagtg
     5281 ggtggcgctg atccagccct tcggacgtta tgctgcattc acgatgatcg ccgggctgct
     5341 tggcctgtgg gcgcgacgtt ttctcgtcga ccgcgtgcgt tatgtgacca cgccgtcgga
     5401 tcatctgatg cttgcgctgc tggtgggtat tggcctgagc ggggcatcga tgagctttgt
     5461 cgtacatacc gacatcattt cgctcaagga attcgcgctc ggactgatgg cgttcgactg
     5521 gcagccactg ccggaagatc cgttgctgct gatccacctg tcgctggtgg cgttgctgat
     5581 gctggtgttc ccgctcagca agctgctgca tgcgccgggc gtgtttttca gtccgacgcg
     5641 caatcaggca gacgattcca ggagccggcg gcacttggcg ccgtgggctg cgcaacttga
     5701 agaatgagac gggggaattg cggtggctac ttccaatttt gaagttccag aactgacagg
     5761 ctgtaccgaa acaccgcgtg tgcaggaagg cgcggcagcc ggaatcggtt cgttcctggc
     5821 caatgccccc atacaggaag cgctcggttt tccgggcgaa ctggtggaag actggcatga
     5881 tcgtgctatc ggaaagatgg gcgaactgct cggcaaatac cgttcgctgc aagtgttcat
     5941 ggatgcctgc gtgcactgcg gcgcctgcac ggacaaatgc cattattttc ttggcacggg
     6001 cgacccgaag aatatgccgg ttgcgcgtca ggaactgatg cgcaaggtct accgccgtta
     6061 ttttaccttc gccggcaaga tgttcccgtc gttggtgggg gcgatcgacc tgacgcgcga
     6121 agtgatcgat gactggtaca agtattatta ccagtgctcc gagtgccgcc gctgttcggt
     6181 gtattgcccc tatggcatcg ataccgccga aatcaccatg gccggtcgcg agattcttga
     6241 cagcatcggc gttggtcaga aatacaccaa cgagattctc ggcaaggtgc accgcatcgg
     6301 caacaacctc ggactgcccg gtccggcact ggaagatacg ctgtccggtc tggaagaaga
     6361 cgtactcgac gaaaccggat gcgatgtcaa gttccccatt gacgtcaagg gcgcagaagt
     6421 gctgctgatc acgccgtctg cggatttctt ttccgaaccg cacgtcgaga gcctgatcgg
     6481 ttacgccaag gtgtttcatg ccgccggtat cagctggacg ctgtcttcca tggcctccga
     6541 ggccggcaat ttcggcatgt tcatcggcag ctatgaccag ttgcgcaagg tggcgctgcg
     6601 cattcgcgag gcggcgcttg aactgggcgt caaacgcatc gtggttggtg aatgcggcca
     6661 tgcctggcgc gtggcctaca gtttctggaa cacgctgatc ggagttgggg ggggtgccga
     6721 cgatgaattt ggccgcatgc tgcaaaagca gctagatggc gcttatccgc aaccggccca
     6781 catttgcgag tacacctacg atctgctgca acgcggcgtg atccaggtgg acaaggaagc
     6841 caacgatcat cgcgtcatta cctttcatga ttcatgcaac gtggcacgcg gcgccggcat
     6901 ggggggcatc cccggagggc agttcagtat cccgcgcgcg ctcatcaagt cggtggccaa
     6961 taatttccat gacatggcgg cagaaacgat ccgcgaaggc acgttctgtt gcggtggcgg
     7021 tgggggtctg ctgaccgacg acctgatgga attgcgtgtc aagggagcgc aaccacgcat
     7081 gaatgcgttg aaaaacgtgg tggatggtga aggcgtcaat ttcatggcga ccatctgcgc
     7141 gatctgcaag gcgcaattta ccaaagtgct gccttactac gggtacgaga tgggcatggt
     7201 gggaggcgtg caccagctgg tgagcacggc catcaagctc aagtgcgata aataacggat
     7261 tacggaatac ttcaggaggc aatgatgtct atcacgtccg gagatacgga aaagagtctg
     7321 aactatcgtc gttacaagga taacgaccac gactggaaac gctggcagga caagatcttt
     7381 gatgccggtc atacgcatct gtgtccggaa tatgtgctgt cgaccccgcc ttgccagggg
     7441 agttgcccgg caggtgagga tatccgtggc tacctgaaca tcgtgcgcgg tgtcgagaaa
     7501 ccgcctgcag gaacggcatg gcaggaatat gcctttcgcc gcatcaccga agccaacccg
     7561 tttcctgcgg tgatgggccg ggtgtgtccg gcaccgtgcg agtcaggctg caaccgcaat
     7621 caggttgaag atttcgtcgg catcaattcg gttgagcatt ttgtcggtga ctgggcgctg
     7681 gccaacaaac tgggtttttc cgccaaggtt gagccgaccg ggaagtccgt ggccattatc
     7741 ggcggggggc ctgctggcct ggcctgtgcc taccagttgg cgctcaaggg tcatgcagtc
     7801 accatttttg atgaccacgc ccagctcggc ggcatgatgc gttatggcat tcccggcttc
     7861 cgtacgccgc gtgacgtgct cgatggtgaa atccagcgca ttctcgatct tggcgtgcag
     7921 gcgcgtctga atacccgtat cggcaccgac gtcagcatgg ctgacctgga aaaacagtac
     7981 gacgccgtgt tcatggcgct gggtgcgcaa agtggccgcg atctgccggt gccaggcttc
     8041 gaggctccca attgcgtgac cgccatggca ttcctgcgcg cattcaacga cggacgtctg
     8101 aagcatgtcg gtcaccgtgt ggtggtcatc ggcggtggcg atacctcgat cgacgtggcg
     8161 actgttgccc gccgcctggg tcagattacc aacgtgcatg agtccgaccg tccggagcat
     8221 gtcattgccg gcaaggtggc acatgacgtg gccgaggctt cggtgcgcga gggtgctgaa
     8281 gtggtgctga tctcgcgcgc cagcctcgac aagatgcatg ccaacaagca tgaaatcgaa
     8341 caggcgctgg ccgaaggcat cgatatccgc tgtggcctga ccccggtatc tgtcacccat
     8401 gacgccagtg gccgcgccat tgcactgcgc gtggccgaat ttgacgtggt caagggtcag
     8461 acggtggtgc gtcccgggac cgagcgtgac attccgtctg atctcatcgt ctcggccatc
     8521 ggccaggctg tcgatttcac cgggctggaa gagttcgaca acaacaatgg cctgatcaag
     8581 gcggacagca gacccccacc gccaccgcaa cagaacgtgc cttcgcggat cgtttctgcc
     8641 gccatgtcat ggaaattatt ggccaccgac ttgatgagcg cgcgcgggat actgaactgc
     8701 cctc
//
LOCUS       DAIANL010000118         5880 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3008059, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000118 DAIANL010000000
VERSION     DAIANL010000118.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3008059"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1179 a         1901 c         1662 g         1138 t
ORIGIN      
        1 tccacatcga atggcttggg cagatattcg aacgcgccac cctgaaatgc ggccaccgcc
       61 gattcaaggt cagaataggc cgtcatgatg atgaccggca tgcgcgggaa ccggctggaa
      121 aactggtgca gcagttccag ccccgacccc ccgggcatgt ggatatcgct caccaccacc
      181 tggggcagcc cggtttccag cgcggcttcc acttctggca cggaagaaaa gctttcgaat
      241 gcgatgttct cgcgctgcaa ggccttttcg aacacccagc ggatggagcg atcgtcgtcg
      301 atgatccaga ccggtttaat catgatgagc cccattcatt gatggcagaa acctgcccga
      361 gcggcagcag gatggtaaac accgtgcggc cggggcggct gtctacctcg atggtgccgt
      421 ggtgctggtg cacgaatgtc tgcgccagcg tcagcccgag cccgctgccg ccctcgcggg
      481 ccgacaccaa cggaaaaaag atgcggtcgc ggatcgaatc cggaatgcct gggccgttgt
      541 cgataatctg cacctgcaac gccagcggat gacgtttcat ggccagcgtc acctggcgca
      601 tgacacgggt gcgcaacacg atctctccct tgccccgcaa tgcctgcact gcattacgga
      661 cgatattcag caccacctgg atcagctgct cccggtcacc gaccacatcc ggcaggctgg
      721 tgtcatagtc gcggcgaatg cgcagcccgg tcggcgtttc tgccagcgtc acactgcgga
      781 cgcgctccag cacttcatgc acattgagcg ggctgaactg cggcagacgg tgcggtgtca
      841 gcaaccggtc catcagcgat tgcagccggt cggactcctg gatgatgacc cgggtatact
      901 cgcgcgtctc cggggccagt tcgcgttcga gcaactgtgc cgctccgcgg atgccgccaa
      961 gcggattctt gatctcatgt gccagactgc gcaccagctc gcggttggcc tgctgttgca
     1021 gcaaccggtg ttcgtcacgc agcaccttga gctgctggtt catctcgtgg aattccagca
     1081 taaacgcgat ctgcagatga tcgaccacgg taaacgtcgc gctgagctgc agttgcactt
     1141 cacctgcctg cagcgtcagt tcgtgctcgg taaaactggc attgtgctca gccgcgtaat
     1201 cgagcgcagc atgcagcgga gccgcatgca cgaacagctg ttccagggtc atgccgcgca
     1261 cctgccccag gctcagggca aacaggtttt ccgcggccgg attgatgtat tgcacctcac
     1321 gcgccgtacc cagcgcgaat accgccgtcg ccagccattc cagaccagaa aactggttag
     1381 tcatcgagtt catgcagtga tcttgcagtc agtgtataaa attccagcca ccttcatgca
     1441 ccgttatcgt gcggacaacg gcagatgcgt gctttacctg ccactacaag caagtaatgg
     1501 tccagaatcg ccacgactgt tccaatgcgg tgcgcgtcag ggaatccggc gcaattcacg
     1561 gttcagtgca tccacgttat cctggtgcaa tttgactgca tcatcgagtt tttcaagccg
     1621 ggactggtag gctgcgccgg ttttctgctc gccaggctgc ggcgtttcgc cctgacgacg
     1681 tgccgcccgg gcctgttcga gcgactgacg ctcggtctgc agttcgcctt caagcaccga
     1741 acgccgcagg ttgtcacgcc gggactgcgt ctgcgtgtcg atgcgcggca cgctcacggg
     1801 ctcaacaacg gctggcctgc tgtggacgga tctgtgcgcg ggcttccagc tcgcgccgga
     1861 cgttccgata ctcagccgct tggcacctgc caccgggtaa tcggtatagg agactgcccc
     1921 ggaagcatcc acatgcttgt aaatctccgc ctgcgtcggc aatgccacgc accagaatat
     1981 ccagaagatt gccaatcgct tcatctgcat cactcgccgt atcaccgcgc tcaagtgtag
     2041 ggcagcgcat gccccggtgc aaccctgccc accacaggtg ctacgaccaa agaaaaaggg
     2101 gcggttttac ccgccccttg tgtttcgtgc tgctcgcggc ttacagcgag tagtacatgt
     2161 cgaactcgac cggatgcgtg gtcatgcgga agcgggtgac ttcttccatt ttcagctcga
     2221 tgtaggcatc gatcatctcg ttggtgaaca cgccgccacg ggtcaggaac tcgcgatcct
     2281 tgtcgagggc ttcgagcgcc tggtcgagcg aatggcacac cttcggaatc ttggcttctt
     2341 cttccggcgg caggtcatac aggttcttgt cggcagcctc acccggatgg atcttgttct
     2401 ggatgccatc gagaccggcc atcagcatgg cagcgaacgc caggtacggg ttggccgaag
     2461 gatcggggaa acgcacttcg atacggcgag ccttctcgct cggtacgtac ggaatacgga
     2521 tcgaggccga acggttgcgt gccgaatagg ccagcatgac cggcgcttca aaaccgggca
     2581 ccagacgctt gtaggagttg gtgccagggt tggtgatggc gttgagcgcg cgggcgtgct
     2641 tgatgatgcc gccaatgtaa tacagcgcca tctccgacag accggcatag ccgttgccgg
     2701 cgaacaggtt cttgccgtct ttccagatcg actggtgcac gtgcatgccc gaaccgttgt
     2761 cgccaacgat gggcttgggc atgaaggttg cggtcttgcc atagctgtgg gcgacgttat
     2821 gcaccacgta cttcaggatc tgcgtctggt cggcgcgctt ggtcagcgtg ttgaacttgg
     2881 tgccgatctc gcactgaccg gcattggcga cttcatgatg gtgcacttcg acttccacgc
     2941 ccatctcttc catcgccaga cacatggccg aacggatatc ctgcaggctg tcgaccggag
     3001 gcaccgggaa atagccaccc ttgacaccag gacggtggcc catgttgcca ccctcgtaag
     3061 catcgccgga ctcccagctg gcttcttccg agctgatctt gaccatggtg cccgacatgt
     3121 cggtcttcca caccaccgaa tcgaaaatga aaaattcggg ttccggaccg aagtaggcgg
     3181 tatcgccaat gccggtggac ttgagatagg cttcggcacg cttggccagc gagcgcgggt
     3241 cgcgatcgta gcccttcatg tcggatggtt cgatgacgtc gcaggtcagg atcagggtgg
     3301 attcatcgcg gaacggatcc agacgggcgc tgtcggcatc gggcatgaga atcatgtcgg
     3361 aggcctggat gcctttccag ccggcaatcg aggaaccatc aaacgcatgg ccttcgctga
     3421 acttgctttc ggaaaactgc gataccggca cactcacgtg ctgctctttg ccacgcgtgt
     3481 cggtaaaacg gaaatcgatg aaccggactt cgttgtcccg caccatctgc atcacatcgg
     3541 cgactgccat tgaaattctc cttgcacgat tacaataaat tctgttctgg gggccaagcg
     3601 gcctgtctga caagcacgat atgtgccaaa aaccctgtcc aacctgccaa catgccctga
     3661 ccggagaatt atgcttgtta aaccgtacgg atgcaaaact tatttgccat gatgcgatcg
     3721 cgggtttggc acgccctgtc agcacaaaat cggtgcgcgc agcaggccgt ctgcaccaaa
     3781 ataacgcacg acacgcctgc caacttactg gactggctac cgtgatgcac gcatagccgc
     3841 aatggcgccg ttccggtaca atttacgcac cgacccagct accggagctc cccttgaccg
     3901 accgctatgc cgtgatcggc aacccgatcg cccattcgcg ctcgccggcc atccatgccg
     3961 cgtttgcccg ccagaccggg caggacatcg actacagccg gctgctcgcg cccctggacg
     4021 gatttcctgc cactgcactg accttctttg gcgagggcgg acgcggcgca aacgtcaccg
     4081 taccattcaa ggaagccgca tttcgtcttg ccacgcatgc cacagcccgc gccacactgg
     4141 cagaggccgc caatacgctg agcttctccg gcggcgaact gctggccgac aacaccgacg
     4201 gccccgggct ggtacgcgac ctggtacaca acctcggtgc gcagctgcaa cgcaagcgca
     4261 tcctgctcat gggagccggg ggtgctgccc gcggcgtgtt gccagcgctg atggcggaag
     4321 acccgctgta cctggtcgtc gccaaccgca ccgccagccg tgctgagacc ctgcgcgacc
     4381 acttccggcc tctcggtatc atcgaggcca gcgactatgc cgatctgcgc ggcctgaaat
     4441 tcgacctggt catcaacgcc acctcggcca gtctagccgg cgaagtccca ccgttgccac
     4501 cgggactgtt cgccgccggc agtctggcgt atgacatggt gtacggcgcc gcgccaacgc
     4561 cgttcatgct tgccgcacgc gaagccggcg catcgcgctg gatcgacgga ctgggcatgc
     4621 tggtcgaaca ggccgccctg tcctttgcac tctggcgcga cgtcagaccc gacacagccc
     4681 ccgtgctcgc cgccctgcgc accgaactca cccacaccca ggcatgaaac gcctgctgca
     4741 tcacgctctg tggatcgtgc ccacggcatt gatcctgtat caggcctggc tgtttgccca
     4801 tgtctggtgg tgggtcgacc acaacccgca acgcagcgcg ctgatggacg aacgcctcga
     4861 ccagttgcag gcacagcatc ctgacgcccg cctgcagcac gaatgggtac cttacggaca
     4921 gatatccgcc aacctcaaac gcgcagtcgt cgccgccgag gatgcccgct ttctcgaaca
     4981 ccacggcttc gactggaacg gcatccagtt cgccgcagaa aaagatctcc ggcgcggcca
     5041 tatcgtcgcc ggaggttcga ccatcagcca gcaactggca aaaaatctgc tgctctccga
     5101 ccagcgcacc ccgtggcgca agatcgaaga agccatcatc accatcatga tcgaacacat
     5161 gatgagcaag cgccgcattc tcgaaattta tctcaacgac atcgaatggg gcaatggcat
     5221 atttggcgca caagccgccg ccagccacta tttccacacc agcgccgctc ggttaggtcc
     5281 atccgaagcc gcccggctcg ctgccatggt gcccaatcca cgttattacg acacccaccg
     5341 caacagccgc gaactgcaac gccgcaccgt aaccatcggg gcacgcatga atcaggctgc
     5401 cattccgcgc taaatccccc gcgtgctcac gaacaccacg agcccacacc cgtggcgcga
     5461 atcatgcttg aatccgggga cagcgcggcc aagtctccgg aatcagccgg ttcgcactgc
     5521 aacatgcaca aggggaaatc accatgagca caccgtcaca tccgcagcac cgcgagcccg
     5581 cgtcccactc catgggtgaa tttgccgcgg ctttgctcat gatcatgctc agcattctgg
     5641 tgttctgggc ctgacatcga atccaccgag gcgcaccatg ccggaccatg cggtctcacg
     5701 atgcactgcc tgcccgcacc cctgacctgc cagcccggga atgcacattg cttgacggaa
     5761 tagcaaaaat ccggtctaat ggcgggcttc tcggctgggt agctcagttg gtagagcagc
     5821 ggactgaaaa tccgcgtgtc ggcagttcga ttctgccccc agccaccaat aaaatcaatg
//
LOCUS       DAIANL010000119         3996 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2187607, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000119 DAIANL010000000
VERSION     DAIANL010000119.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187607"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          663 a         1156 c         1315 g          862 t
ORIGIN      
        1 tggggcagga cgagattcgt catgttccgg ttgagattgc gccttttgcc tcgtatcctc
       61 tcgatgtggc cacgttgttt cccgaagcac gctggccgca gcagttcgag gtgcaggcgg
      121 gaaagcattt cgtgcgcccg cgttatgaag ttcaggtgcg tggcggacgc taccgcattg
      181 cgcatgtcaa cgtcgaacgc accgacctgg ttgcggaccc acgcattgcc gaactgacgc
      241 gctggatgga gaaatcctat ctgctgccgg cgccgatttt gccggtggcg cagtggaaca
      301 gcgtgatgca gcccaacccg atgtcgaccg ctcagcgcaa tctgccggtg gcggcgattt
      361 tctatgatgc caatggcacc gaagccggac ggcacttttt tggctgcctg ccgcgcgacg
      421 cgagcgtgtc ggtcgacatc aatgcagtac tcgaagcgca gaccgccagt ctgcccagcg
      481 gcagcggcca tgtcgaactg gtgtatgatt tcagcgaggg cggcgaggcc gatggctggc
      541 tgcacgggct gtttcgctac actcagcggg cgagcggcca catggccgag accagtttcg
      601 gcgcacacat ctacaatacg gtgctgacct accgtaacga accgcagtca tacatctctg
      661 ttgcgcccgg cctgagtaca cggctgttcc tgcgtttggg cgatacgccg ctcgacacct
      721 tctgccacct gatctatccg gcttcttcgt cgtggcacga gcattcggat acccagctga
      781 cgctgttcga ccgactgggg cgtcaggtag cgcagacgcg ggtgcgtatt ccgtgcagcg
      841 gatcgctgca ctggcgttac agtgaggtgt tcgatgccgc cgtgcgcagt caggctggaa
      901 acggcgctta catcatcatc cgtgacctga cctgccgcct gttcgggtat catggcacga
      961 tcgccggtga tacctcgttc tgcatggatc acatgttcgg attctgagcc atgaacacta
     1021 taaccgtaac cgacctgcgt gctaccgaag cgagccagtt gcaaggtatc gtgcatgaaa
     1081 ccgttcgcgt tctggaacgc gacggtgtgg tcagactgcg tttcgccgaa gatccggaac
     1141 tgctgttgcg cagtgtcaac ctgcgcttgc gcaacaacct gtcatggcag gtgcggcagg
     1201 agctgggggc gggctgggtg gcagaggtgc accgggctga agacgttccg ccccatgacg
     1261 tgctcgacgc actgcagcgc gaccacaaac gcatcgatgc gttgttttcg cgtgtgatcc
     1321 atttgaccga ccgcaatgaa cttgtcgctg ctgctgacta tatggaacag ttcgttgtcg
     1381 ccatccgtcg tcacctggtg gtggagcacg acatgctggc gcgtgcgatc cgtacccgcg
     1441 cggatgcgac cggggtcgat ccttcagccg caatgatcga cgaacaccgc gaaatcctcg
     1501 cgcagtcggc aatgatcctc gccgccttcg aggaaaccga tgccagtgcc gccagcgtct
     1561 cgccgttgct ggcgattctg gcaggctatc tttccaagca tgaggttcgc gaggaaatga
     1621 cactgtttcc catctggcaa cgtgcactgt cgcacgcgcc gcaaccggac cgttcggcat
     1681 tgttcgaacg ggtgctcacc caactggaac tgtaggctcc cgatgtcgaa cttttcgcaa
     1741 caagcccttg attatcatga atttccagtg ccaggcaagc tgagtgtcga atcgtccaag
     1801 ccgtgcgaca cggcagccga tctggctctg gcctacagtc ccggtgtggc cgagccggtg
     1861 cgtgcaatcg cgcaatcgcc tgaatcggcc tggcgttaca ccaacaaggg caacctggtg
     1921 gcggtgatca ccgatggcag cgccattctc ggtctgggca acctggggcc gctggccgcc
     1981 aaaccggtga tggaaggcaa ggccgtgctg ttcaagcgtt ttgccggtat cgatgtgttt
     2041 gacattgaaa tcaacgcacc cgatgtggct tcgtttattg aaacggtggt caacattgcg
     2101 ccaacctttg gtgggataaa tcttgaagat attgctgcac ctcattgttt tgaaatagaa
     2161 aaggcactgt ccgaacggct ggacatcccg gtattccacg acgatcagca cggcacggca
     2221 gtcattatct gtgctggtct catcaatgcg ttgcacatcc agggcaagac gctcgatcag
     2281 gcgcgcatcg tttgcctcgg cgcaggggcg gcgggcaccg catcgatgaa actgctgctg
     2341 gcgctgggtg ccgaccagtc gaagctgctg gtggttgaca aggtgggtgt gctgcatcgt
     2401 ggtatgcacg acctgcccga acaccaccgc ttttttgccg ccgatacgga ggcgcgcacc
     2461 ctggctgatg ccatggcagg tgcagatgcc tttatcggcg tgtccgcggc caatctggtc
     2521 agcgccgaca tggtgaaatc gatggccgag cggccggtgg tattcgcgct ggccaatccg
     2581 gatccggaaa tccgcccgga agtggcgcac gctgcgcgcg atgacctgat catggccacc
     2641 ggacggtcgg attaccccaa ccaggtcaat aacgtgctgg gtttcccgtt cattttccgc
     2701 ggcgcgctcg acgcgcgtgc accgcgcatc acccaggcca tgcaggtcgc agccgtacat
     2761 gcgctggccg ggcttgcgcg cgagcctgtg ccggacagcg tgcttgccgc ctacggtatc
     2821 gatcggctcg aattcggtcg cgagtatatt ctgcccaagc catttgatcc gcgcttgcgc
     2881 gagcgcattc cggccgcagt cgctgcggcg gctccaaaac gctgacctcc gatgccggcc
     2941 cagcagtccg ttcgctggtt gcgaccgtgg caggtgtggc tgccgttacc cggctggctg
     3001 tccatcctgc agcgtgtcag cggcctgctg ttgatcctgt tgtttccgcc gctggtgtgg
     3061 ctgtttggtc attcgctcca gccacacggc tggacggata tcgtgcactg gctgtatgcg
     3121 cttcccgggc gttgtgcgct gacgctgctg gttggagtcg ttgcgctgca cagcttcggc
     3181 ggcctgcggt tgctgttgct cgatgtcggt atcggtaccc gcttgccgct ggcgagacgg
     3241 ttgtcatggc tggtgttggg actgacctgc gcgggcatgc tgtgggcggt cgtgggcgtg
     3301 tcatgaaacg ctggctgctg cagcgcatca gtgcgctgat cctgctgttt gcgctggctg
     3361 ggctgggatg gctcgtgctg gtagcggcac cgctggacga aatgcactgg cggcagttgt
     3421 ttgccggggc tccggctcgt gtgctggccg gtctggcggg gttcgcgctg gcggtgcatg
     3481 cctggtgcgg tttcgaggac atgatcggcg attatgtgcg cgcggcggtt gcccggcgcg
     3541 tgctgctggc ggcgggtgcg gtctggttgc tggctggact ggcgcagctg gtgtctgtgc
     3601 tgcggtgagg gcgacgatgg atgtgatccg gcatcgtttc gatgcgctgg tggtaggggg
     3661 tggcggtgcc ggactgcgtt gcgcgctggc gctggctgag gccggacggc gcgtggccgt
     3721 ggtgtccaag gtgatgccac tgcgttcgca cacggtgtcg gcgcaggggg ggatcactgc
     3781 tgctctgggc aacatggagc cagacgactg gcgctggcac atgttcgaca ccgtgaaagg
     3841 atcggattac ctgggagatc aggatgccat cgaattcatg tgtcgtcagg cggcgcaggc
     3901 cgtaattgaa ctggagcaga tggggttgcc attttcgcga cgcgaggatg gacgtatcta
     3961 ccagcgtgcg ttcggcgcac aaagcgtgcg tttcgg
//
LOCUS       DAIANL010000120         6975 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2396806, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000120 DAIANL010000000
VERSION     DAIANL010000120.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2396806"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1333 a         2409 c         2038 g         1195 t
ORIGIN      
        1 cggcattgtg cttgatgatc accagattgc cataaccgtg caacccggat ccgctgtaca
       61 ccactttgcc gtctgccgca gccacgactg gttgcccggc ggcaccagca atatcgatgc
      121 ccttgtcacc gcccgcatcg ccaaatccgg ccacaacctt gccgtctgcc ggccagatcc
      181 atttgggcat gccggccggc tcggtcgctg cggcaacggc cgccggcgcc gcagacgcag
      241 ttgtcgttgc cggcgcaact gccggcaccg gagcaggtac cggcgcattg ccggactgct
      301 ccgcctgccg caacgccgcc ggcgaataag gcagctccac ggcctgcggc tgatcaaccg
      361 taaccaccac tggcaccgcc tggaccgcga gtggctgtgc atgcagcggc accgtctgcg
      421 ccgcgtcagc cccggcagca ccgccgcccc cctgcaccgc ctgcgccccg ggtgcggcca
      481 gccgcaacgt ctgacccaca tgaatccggt tgctgtcttc cacaccgttc cactgcgcca
      541 gctcgcggta atcgagtcca tggtcgagcg caatgctgta caaggtatcg cccttgcgca
      601 cgacgtaggc cggaccgccg gctaccgcaa ccggcgctgc aggcgcagcc atggccgtca
      661 ccggctcccg tcgcgccagc gggctcgaca ggtcggtaat cggcgcacgg ttgttcgccg
      721 tactgcaccc ggccagcgcc agcagcagcc cacagccggt cagaaaagac caccgctcga
      781 tacgttgttc cattacgcca ctcctggcag caaaggcaca aaatgcacca tctcaagcac
      841 ctgttcctgg tagctgtcgc cttgccgttc gatcagcacc atgaactggt tttccccgac
      901 cccgcgcggc agcaccatgc gtccaccatc cgccagctgc gccagcaacg ccgacggcgg
      961 cacggcagcg gcggctgcga gcacgatacc gtcgaacggg gcgaattcgg ccagaccagc
     1021 attgccatca ccgtgtttga gcttgatgtt ggacaacctc agttcgcgta tcgtgcgccg
     1081 ggcctgtcca agcagggtgc tgatgcgctc gacagaataa acttcccggg cgattctgga
     1141 aagtactgcc gcctgataac cacagccact gcccacctcg agcacgcgct gcatctcccg
     1201 gccatcgcgc agcacttcga gcatgcgggc cacgctatac ggctgggaaa tggtctgtcc
     1261 caggccaatg ggcagcgcgg tgtcctcgta ggcgcgaatc gccagcgcct cgctgacgaa
     1321 cagatgtcgc ggcacctgtg ccatggccgc aagcacggcc atgtcgcgaa taccctgctc
     1381 gcgcaaccgt tcgaccatgc gcgcccgcgt acgctcggaa gtcatgccga tgccgctgtg
     1441 ccggatggaa ttcatcgtgc aagccacccg cccagctcat cgagccgctc ataccaggtc
     1501 agatcgatct gcaacggcgt gaccgagacc agcccgttgg caatggcgtg aaaatccgta
     1561 ccttccgagg cgtcctgcgc cggaccggcc gcacccaccc agtacaccgt ttgtccgcgg
     1621 gggttgagcg tgcgaatcac cggttcagcc ttgtggcgcc gtccaagacg ggtatgtcgc
     1681 atgccgcgca gctcggccat cggcaggtca ggcacgttga cattgagcag catcggcgac
     1741 tgcaacgggg cgcgcagcaa acgctgtacc agctccagcg tcacttgcgc cgcggtatcg
     1801 aaatgttcgc gcccgccacc cgccagcgac acggcaatgg acggcacacc gagcaggaac
     1861 ccttcggtgg ccgcggcaac ggtaccggaa tacacggtgt cgtcacccat gttggcgcca
     1921 tgattgattc cggagatgac catgtccggc aacgcgtcga gcatgccggt cacggccaga
     1981 tgcacgcagt cggtcggcgt gccgttgaca tagaagtatc cgttatgcgc ttcgcgcagg
     2041 gtaagcgggc ggtccagcgt cagagaattg ctcgcgccgc tgcggtcgcg ctccggcgcg
     2101 accaccacga ccgtggcaat ggccgacagt gcattcgcca acgcggccag ccccggagcg
     2161 aaatatccgt catcgttgct taacaggatg cgcatgagtt ggcggcggtg tgatgtctga
     2221 caggaaacag ccgcattcta caccaaccac ggcaaaacat gcgcaaccag aataagtacg
     2281 ccgcatggac ggcgtacagc tgtcagaccg ccgtattcac cattgccccg gacgcctggc
     2341 tcgagccgag atcctgcatc aggttctgca ggaaggattg caggctgggg gtgccggccg
     2401 cagccgcact gcccgtcatg cccggtgcag tcgtgctgcc cagcgccgcc accagattct
     2461 ggaaactgtt ctgcaggttg cccaccgcgc ccatcgacgc gctggccgaa gcggtattgt
     2521 tgcctgccgc actgctgccg gacaattgct ggagcagttg ttgcaggtcg gctgcaaaat
     2581 tgccgtggtg tccggcgtga tgggcaccgg ctgcggcaac accagatgcc tgccctcccg
     2641 atacgcccga agccgcctgc tgcatggcag caaccaggct ttgcatgaat gactgcatgg
     2701 ccgcctgcgg cgactgggtt gcggacgacg cgccgctggc agcattgccg gcagccgcaa
     2761 tgctgttcag gttaagaccg ctttgcgcca tggcctgctg cagtgcctgc aggaacccgc
     2821 ctgccgcccc actggctgcc gagacaccgg caccccgatc cccatcacga tcgctgccgc
     2881 cggtcttctg tacgcccgtg ctgttcgaaa accaggccga cagattcgac acccccgaca
     2941 tgccactgac tgtgctcatt tctttccctc cattcggcaa tgccctgcaa cagcctgcgc
     3001 ctgccgctcc gccatgcgga cggacggtgt gccggccccg gtgcgcgaac gacagtgcgt
     3061 cagccgcgcg gctgaagaca gtatcgcgcg caatgcatac aatccatcgg caaaacagag
     3121 ccggttttca ccccctgttc ttccgcttcg cgcatgtgcc gcgtcagaca catccggaac
     3181 ccgcaccgga tagccttgct caaggacgcg gtgattgatt ctccacgggt cgcgttgtgg
     3241 gtgaaatcgt gatgatcgtg agccgaatga cgcagatcgt ggccaggact acgacacccg
     3301 ggaacacggc aaaacgagcg cacgatttga tcgcaacccg cacgggacgt ggtctggcgg
     3361 gcggtttgct gcgttgtgct tcttgtcggg gggatcagcc attgactgcg aatcacgcct
     3421 tgccacccgt cccgtcagac cggtgtcgcg atcatgcgga tttaatcacc gcgtccctaa
     3481 cgcacccgcg ccagctcctc ccaacgcgtg gtataggccg gactgacatt gccgcggcgc
     3541 atgcgccagg caggatcgat gccttcactg gccaaccgga gtgtaccgct gcccatgcgc
     3601 gcgttgatcc ggtcgagcgt ctgcatcagt tgcgcacgcg atgcctcgcg cccggtatcg
     3661 gcaaacaggt cggcctgcgt ctgcccggcc ggctgcaaat cggacaagac cacgcccgcc
     3721 ttgcggtaag cgaaaccggc gcgccagagc tggcgcaccc cagccagcgc tgtccggacc
     3781 agcaacaggg tgtccgcact cgggcgcagc agtccgatac gcatggaagg cgcgtgttgc
     3841 ggtgcccgga cgcgatgcgg atcggtgcgc aggaatacct gcaccgtcga tgccaccgat
     3901 ccctggctgc gcagcttgtg cgcggcgcgc gccatgtaac tgctgatggc ctgttccagt
     3961 tcgtcaagtc cggtgacctc tcgaccgaaa gaacgcgagc tgatgatctg ctgccggggc
     4021 gccacgacat cggtcaggca aaaacaggct tcaccacgca attcgcggac gatacgctcc
     4081 agtgccacgc cggaaccctg ccgcatcccc tccgtacgca tggcgcgcag ttgccacacg
     4141 gtatgcacgc cccgttcacg cagacccgca gccagccggg gacccacgcc ccacacctcg
     4201 tccgcgtcga tgccctggag caaggcctgc cattcagcct gtgccatggc gccgaagtca
     4261 cacacgccct cgcggcccgg ctgccgcttg gcgacatgcg cagccagctt ggccaacgtc
     4321 atgctcgaag cgatccctac gctgaccggc aatccgagcc agcgggcaat acgctgacgg
     4381 atgcgccgcc cggtctcggc cggcgcctct ccgaggccgg tcagatcgag aaagcattca
     4441 tcaatgctgt agatatcctg caccggagca aactgcgcca acacctccat cacccgattg
     4501 gacaagtcgg catacagtgc gtaattcgat gaacgcgcca ccacgccgtg cctgtgcgcc
     4561 agcgcgcgca ggcggaacca gggctcgccc atgggaatac ccagcgcttt ggcttcctga
     4621 ctgcgtgcaa ccacacagcc atcgttgttg gacagcacca ccactggccg cccatccagt
     4681 ctcggatcga acagccgttc ggcgctgacg tagaaattgt tcacgtccac cagggcaatc
     4741 gttgcgctca tccacgccac ctcatgacgc ctcgagaaac catcgcaaac cagacaagat
     4801 caaggcgcat cgcgcgcagg atacgacccc caccagatgg gcaggcaagt ggtcgggcac
     4861 agagcagcgc agcaattggc tggtgaaacc gttttttcaa ggcacccctc attccacgtt
     4921 gtggatggca tggacaacca ccccccagat caccagttcc tgcccttcgc gcaaacggat
     4981 cggcgcatag cgcgggtttt ccgcatgcag ctcgatgcgg ccatgctcac gaatcagccg
     5041 cttgacggtc aactcgctgt cgaccacggc caccaccacc gaacggtgca ccggctcgac
     5101 ggaacggtcc accacaagga tgtcgccgtc gtggatcccg gcggcaatca tcgaatcgcc
     5161 ctgcacgcgc agaaagaacg tcgccggccg gttacgcacc agcaactggt tcaggtcgag
     5221 ccaggtttcc acataatcat ctgccgggga cgggaagccg gctgcaatcg catgcccgta
     5281 gagtggcgag cgcgcctccg gtgcgtgcgg gtcgggccag gccagccagt gcaccacact
     5341 ggccggtctg tcggaatcgg aaggctcgtt catggcgggt ctcttcaggg aaacagggta
     5401 tcctgcacgg ggtcttccgc gcgcgtcgca agcgcctgca acgccccgag cagttcaacg
     5461 atacggtcgc gcaaccgttg catttcctcg cccagcacgc gcgcctcgtc gtcgcgcccg
     5521 gcttcgtgca gttgcagcac ctccatcccg acacggtgca cctcgacatg caccggctcg
     5581 atggcgcgaa acacatccag atgcccgtaa cgtttgtgac caagatcgtg gtaccacttg
     5641 ccaaagcggc aatgcgcagg gtcgtagtac agcgcatcgc tcttgcgcga ctcgccgccc
     5701 tggatgatcc gcagcacgtg gccgacccat tcgacgtgat cgtgccgcgc gaccagcagc
     5761 ggaaaatcca ccatttccca gttctgaaac tgccacagcg accatgccag cgattcgcgg
     5821 ttgtgccgca accagccggg taccacctcg cccggcatcg gccgcgcgat gccgtaacct
     5881 tgcgccaggt cacacccgag cttcaccagc acataaccat gctcgatcga ttccactccc
     5941 tcggcaatga cttcgcgctg aaacacccgc gccagcgaaa tcgtcgcctc taccagcgcc
     6001 aggtcattgg ggtcatccag catgtcgcgc acaaaggcat gatcgatctt gagcgtatcc
     6061 accggaagat gcttgagaaa gctgagcgag gaataaccgg tgccaaaatc gtcgatggaa
     6121 aaccgcaggc cgcgctgttt gaattcgtgg atgagctggc cgacgtgctc gaaatcctcg
     6181 atcgaggcag tttccaggat ttcaaactcg atgcaggaat gcggcacctc cggatgccgt
     6241 tccatgatgc ggctcatgcg cgcggcaaaa tcaggctgca tcaactggcg tccgctgatg
     6301 ttgatgctga ctttctgctg cccgaccccg ttgtcttccc agcggcggat ctgcaacagc
     6361 acttcgtcga gcacccactc gccaatctcg atgatcaggt cggtatgctc gacctggggc
     6421 aggaactgac cgggcgcgag cagcccgcga tcgggatgtt gccagcggat cagcgcctcg
     6481 aacccgatca cctcaccggt ccgcatgttc agcttcggct gataatgcag acggaactcg
     6541 cgatgctgca gggcctgctg gatccgttcg aacagctgat agcggctctg gatggcggca
     6601 tcgctctcgg aatcaaaaaa atgaacgcaa ttgcgtccgg actgcttggc ctggtacatc
     6661 gcctgatcgg cctgacgcag caagatgtcg gcatcgggaa tgcgcgcgcc gccgcccacc
     6721 accacaccga tgctggcggg tacctcgaac gtctggcgct cgaccacgat cggctgcgac
     6781 aggttctcca gcagccgatc caggatctgg cgcacctcgg tctcgccatt aatcgaattg
     6841 agcagcagga tgaactcgtc gccgccgatg cgtgccaccg tgtcctccac ccgcagcgac
     6901 gattcgatcc gccgcgccac ctcgatcagg atattgtcgc cgatctcgtg tccgtaggca
     6961 tcgttgatct gcttg
//
LOCUS       DAIANL010000121        10101 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_971785, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000121 DAIANL010000000
VERSION     DAIANL010000121.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971785"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2167 a         3437 c         2788 g         1709 t
ORIGIN      
        1 ctacaacgac tcgatgcgcc ggcactatgc cgatttcgat accagcccgg aagtccgact
       61 gactggcctg gacgatgcca gcctgacgct ggctgcacgc cagatcctcg acctggaagt
      121 ccgatcggac atgccatgag caacagcgcg ttcactgcca tgcagacacg gtctgccgca
      181 gtgcatcagt ggcgcccggt gctgccgaac ccgccctcgg cgcgctcggt cgcatcgaat
      241 gcctgcacca cgttgaactg cgcctgaacc acgggcacga acaccaactg ggcaatgcgt
      301 tcgagcggat tgatggtgaa cccgctttga ccgcgattcc acaccgaaat gaaaatctgt
      361 ccctgataat ccgaatcgat cagaccgacc agattaccga gcacgatgcc atgcttgtgc
      421 cccagtccgg atcgcggcag cacgattgcc gccagatcat cgcggccaat gtgcaccgca
      481 agaccagacg ggatcagcag cgtctggtcc ggggcaagtt ccacgggttt gtcgatacag
      541 gcacgcaggt caatccctgc cgcccctgcc gtggcatagc ctggcggatg ttcctcaaga
      601 cgtgcgtcaa gaatcctgac atcaatcact ggtttcatgg ggttgggctc cctgacccgg
      661 tcggttgagc atgccagcca gatgcgccac cagccgccgt gccagcacga gcttgtcggc
      721 ccgtcctagc gcatgcgcac cgcgctcgtc cagcagcgtc agctcgttgt cctcgctgcc
      781 cagcgcgacc tgggccagat tcaccgccat cagcggcacg cgtttggcgc accgcttggc
      841 ttcgccatac tcgagtgcat gttccgtttc ggccgcaaac cccacgcaga acggcggctg
      901 ctcgcgcgcc gcaatccggc cgaggatatc ttcattgggc agcagctcaa gccgcagcgg
      961 cgcagcggat ttcttgatct tttccgtctg cgtccgtgcc ggacggtaat ccgacacggc
     1021 cgccacggca atgaacacct gcatgcccgc cacgcgcgcc tccactgcag acagcatctg
     1081 tgctgcactt tcgacgtcca cgcgctcgac gccagccggt gtggccagac acgtcggacc
     1141 actgaccagc accacctgcg cgcccgcttc ggccgcggcc tgcgccacgg catagcccat
     1201 gcgccccgag ctcaggttgg tgagtgcccg cactgggtcg agccgctcga gcgtggggcc
     1261 agcggtcacc agaacacgta cccccgccag cacctgcggt gtcgaacctg cctcgggcac
     1321 atgtgttgcc agcgccgcct ccagcgcctg caccaactgc accggctcca tcatgcgccc
     1381 cgggccgtcc tctccgcagg cctgaatacc gttctgcgga ccaaaacagc gcacgccgtc
     1441 ctgctccagc acgcgcacgt tgcgctgcgt cgcaggattg cgccacatct gccggttcat
     1501 cgctggcgca acgaacagcg gacaatcgcg cgccagacat gcagccgaca ggacatcgcc
     1561 cgccagaccc tgcgccagtc gcgccaggaa atcggcgctc gcgggcgcaa tcagcatggc
     1621 atcgcactgg cgcccgagat cgatgtgcgc catgccgtgg ccgttggcga ccggctcgtc
     1681 gagcgcgacc ggacgtcccg tcagcgcctg gaaggtcagc gggccgacga agccggttgc
     1741 tgccggcgtc atcgccacct gcaccgcggc gccagcggcg ccaagcaagc gcaccagctc
     1801 cgccgccttg taggcggcaa tgcccccggt cactcccacc acgatacgct tgccctgcag
     1861 cgacatgccg caccttccat ccatcacgat gaccgtcagt ataccagcac gcaccatggc
     1921 atcgccacgg acatcccgat gtgatatcat tgtaaacaat tgaaacagcg aagccgacca
     1981 cgaccaggca tgcccatcca cgattggccc acaggagaac gtccacgcga aaaactgctg
     2041 cagcgcggca cccaggcgct cagcgatgca gagctgctgg ccatcatcct gcgcaccggc
     2101 ctgcccggaa agaacgcgct cgatctcggc cgtgaactgc tggggcattt cggcagcctg
     2161 acgcgcctgt ttgctgccag ccagaccgaa ttctgtgcca ttgccggact cggtgaagcg
     2221 cgttataccc agttgcaggc catgcaggaa ctgtcgcgac gcgctctggc agaacccttg
     2281 cgcgagcgcg acgcgctgac ctcaccggcc caggtacgca attacctgcg cttgctgttg
     2341 ggcggacgcg agcacgaggt gttctgtgtc atttttctcg acagccagca tcgcgtgctg
     2401 gtcatggaag aactgtttcg cggcacgctc ggccaggcca gcgtctatcc gcgagaagtg
     2461 gtaaagcgcg cactggcgca caatgcagcg gcgctgatcc tggcgcacaa ccatccgtcc
     2521 ggagccgcca cgccaagcca ggccgaccgc gtactgaccg acacactgaa acaggcgctg
     2581 gcactggtcg atgtacgtgt actggatcat tttgtcgtcg ccatgcatga cgtgacatca
     2641 tttgccgaac acggctggtt atgatgcaga tcatccggcc gacacccact ggacagcacg
     2701 caagcgacca cgaccagcac cttgccactg cagcgctgtt gcgccgaccc cagacgcgcc
     2761 tctagcagac cgttgaaaaa ggtagcgagg acgcccggat gcaaggcgcg agaaacgcaa
     2821 cgaccagaca tatcaaatcg ataggcgtgg gagtgagtat cgcagcaacg ccgcagacgg
     2881 gtgccgcagt actttttcaa cggcctgcta gtgcaaccgc ggccgctctc cggtgagcat
     2941 aaactgcacc agatcgcgca ccggccggat ttcttcgccg aacggcagcg gctccgagcc
     3001 gcggaagaac agcccttttt ccacgtcccc cgtcagcgct gccgcaagct gggaatcgat
     3061 acagaactga ccggaacggg caataccgtc gcgcagaccg cattgcgtca gacaatgcag
     3121 acccgatgcg cagcggtccg gacggctttt ggcatattcc tgcaattttg cctcgcggcg
     3181 gaggtagttg cgcaaccacg gcgtcagcac cgcccgcgcc ggcaaaccgg caacactcat
     3241 gaaggtaaca atatcctctg ccttcgcctg cgccagcaca cgtttgaaat tgggatgagc
     3301 atccccctct tcggtcacgg cgaacgccgt tcccatctgc acggcagacg ctcccatccc
     3361 gatcagctcg cgcaggcgtt cgtgactgtt gataccgccg gccggaatga gcggaatacg
     3421 ttccttctcg atacccaatt cgcggaacag ctcgagcacc gattcgagca caccgggaaa
     3481 atcaaaccgc gcatgaccga tgtcgtcggc gttgggcgca cccagatgcc cacctgcgta
     3541 acgcggatgt tcaatgacga ttgcatccgg cagccgctgc ttgcgcatcc atttcttgag
     3601 cagaatggca ataccgcgcg catccgacag gatgggaatc agcgccacat cgggaaaatc
     3661 ctgggtcagt tcgggcagat cgagcggcag gccggccccc atcacgatcg cctgcgcccc
     3721 actctcgcac gccacgcgca ccagttccgc atgttcggtc acggccttca tcacattcac
     3781 cgcgaccaca ccgcgtccgt gcgccagatc aagcgccata cgcacttcgc gccgcagcgc
     3841 ctcgaaattg agctgatcaa gcacggcctt gtcgcgggca tggcgcgcct gctccaccag
     3901 atcggcatga tgatggtgca gatcgatact ggcgatcgta cccgtagcgc caagcgctgc
     3961 caccgagcct gccagccgat gtgccgaaac gcccaccccc atgccgccct gcacgatggg
     4021 cagcaactcc cgtcccttta tagaaaacag gggcaaagca gattcggaca taattatttt
     4081 atccccaggc aatcggacag ccggaacatt cttcagcaat ttccggctga ataacgagaa
     4141 gtcaaagtct taccgagtat atcgcaaaat cgtccgaagc caaaatcacg gcaaccaccc
     4201 accgagcgaa tcccggtgag ggccggatgc cgcacaacat ccggtcgaca ttgtgaccct
     4261 ccggcgtcat gcaaaatcag ttgaccacag gaccagcatc atgatataaa ttcgcctttt
     4321 ccgaattcag cgaggaagtc gaaatggctc gcgtatgtca gatcacgggc aagtccccca
     4381 tggtcgggaa caatgtgtcc cacgccaaca acaaaaccaa acggcgtttc ctgccgaacc
     4441 tgcaatcccg ccgtttctgg ctggaaagcg aaaaccgctt catccgcctg agagtgagtg
     4501 ccgccgcctt gcgcaccatc gacaagaagg gcatcgaagc cgtgctggaa caactggccg
     4561 ccgaaagcgc caagcaataa tctgacagga aaccagaatc atggcgagca agattcgtga
     4621 aaagatcaag ctggaatcca ctgcggggac cggtcatttc tataccacca gcaagaacaa
     4681 gcgcaccacg ccggaaaagc tcgagttcat gaaattcgac cccaaggcgc gcaagcacgt
     4741 gctgtacaag gaaatcaagc tcaaataacc ccatcgctgc cgtgcacacg gcagccgtac
     4801 gggctttccg ccgggccggt tcctgcacac agaacaggat gccggacatt cggattgcaa
     4861 caataactac agtatcatac agtcattcgg tagccgattt cgggaacaac caccggaacg
     4921 gcgtcgaatt gcctgattgt gcgcttgttt tcccggccat gcgatgcccg tattgccaga
     4981 aacgagtcat tccggattgc agaaagccaa cccatggttt cgatcgaaaa tcgaattctg
     5041 ctgttttccc tgtgcaccat cagcgcaacc attgcattca ccttcctgtt cgccgcctcg
     5101 agtgaagaac gcaacggaat gcgcaaatgg gcatttgccc tgatctcgga ttcggcatcc
     5161 tggctgatgt tcgccctgca cttcgaatct gccggcacca tggccatgct gctgctgacc
     5221 ggagccatct ttctgtcgat tctggccctg agcttcaaac tggccgccat ctacgaatac
     5281 tgtgaccaga cctcgccacg ctggctgcct gccttgccac tggctgcact cgccatcaat
     5341 ctcgtcttta tcgcaaccgg tcccgaatgg gcgcgcatca cattcaacag cctgatgttc
     5401 gcggcgctga ccctggccat cgcactgagg ctgaaacacg acccggttca cgagggcagg
     5461 cggccacgcc tgctggtaac cggctcgcaa tacgtcatcg ccatcctgct gctgctgcgc
     5521 gcgaccaatg ccatacacgg aggcagcctg cgtttccccg gtctgtcgag caccgtctcg
     5581 caaagcatcg gtttcctgct catgatcagc ctcagcatca ccgccaccat gggattcatc
     5641 ctgctgctca agcggcgcag cgaccaggag atccgctcac tggccatgac tgatgtgctg
     5701 acgggtatct acaaccggcg ggccttcatc cagcgcgccg aacaggaatg tgcacttgca
     5761 cagcgcaacg gcattccgct ggcactgctg atgattgatg tggaccattt caaacagatc
     5821 aatgacctgc acggccaccc gatcggcgac aaggttctgc tggagattgc caccctgctt
     5881 ggtgaacgat tgcgcaaaca ggatacgctg gcgcgctacg gcggagaaga attctgcgtg
     5941 ctgttgcccg gcaccgatga aaccggtgcc gtcagcatcg ccgaaaacct gcgccggacc
     6001 atttcggcaa catcattcaa cgcgagcgcc gcagccatct ccaccaccat cagtatcggc
     6061 atctcggtac gcgcaccgtc gcacacgccc ttccaggaag atttccactg cctgctttcg
     6121 gccgcagacg ccgcgctcta ccaggccaaa cacgatggac gcaactgcat tgtcactgta
     6181 aacagcctgc gcacgacgca gtgctgacag cgtatcccgc gccagtcgcc actcggcacc
     6241 gggcctcagc cctccgctgg catggtacgg taccaggaat gatcggccca ggccagcaat
     6301 tcccggtcgc caccactgtc gccataagca tacaccgtcc agtccgagcg ggctccaagc
     6361 cacgcttcca gccgacgcac cttttccatg ccacggcagt tggctccggc cagccgaccc
     6421 gaacagcggc catccgtcac ttccagccgc gtcgccagca cgtcatccac gcccatactg
     6481 cgcgcccagg gcaacaggta catttcgagc gaagcactga cgatgacgca gcggtgcccc
     6541 tgcgcctggt gccagcgcag gcgcgcaagc gcctcgggac ggatcatcgc tggcaaatgt
     6601 ccggccgcaa agcgacgcgc atgcgattcc atctgcgcct ggctcatccc acgcacgaac
     6661 tggcgcaata cagcggtctt cgcacgcgtg ctgccgatca gcccggcacg aaaagccagc
     6721 agcagcggca gcagcagcgc catgcggcgc cagtagccgg cccggccggc cacctggcgc
     6781 agaaatggca gaaaactgtc cgcgctgctc agcgtgccat caaaatcgaa ggccgcgacc
     6841 acctgaccac gggttgcatt gttgtcactc atcgcgcaca taccggcgcc gcaaaatacc
     6901 gctcgcaaaa tccgcaacgc aacatcggca gccagtcggg cacgcgataa tactgcgctc
     6961 gcacccgggc cagcaccccc tgacgataag ccggataatc aaagccataa ccacgcgcaa
     7021 ggtagaacac agcgccatcg acgatgatgc gtcgttgttc gacgtgcctg aaatatggcg
     7081 catattgcgt cagtggcgtc ggtgacttat cgaatacgag aatattgcgt ccagccaggg
     7141 cacggaaatc cgtcgtcatg tcatcctgcc gggcatgctt cgaaccgggc ccaaacacga
     7201 aatattcctg cccggcatgc cagctcatca gtgccgaagt ggaatagtca ttggtggcca
     7261 gtcgaaaccc atcggcaaac ggccgcacgg cggccagtac cgactgcgga tgcaccagca
     7321 gcacatagcc actgtaaaac cgggtcgact gccaccatgc cgcgggtgct gtcacaatcg
     7381 ccgacagcac cagcgcatgc actacggcga gtcccagcat gaaccggacg agcccgcgca
     7441 aacgtggccc ggatgttctc aggccgatca tgagaaacag aaagggataa aacgccagta
     7501 cccagtgcag cccgatggtc tttcccagcg acagcagcag aaaaaatgcc gtcggcaccc
     7561 agaacaaaaa ggcgtacaag cggttttctg tcaccgcacc cggcaccacg tcgcgccggt
     7621 cacgccacca ggcccacaac accggtggcg tggccagata cagcaccgtc accagataga
     7681 gcccggtatt gtgcagcgag aacgtactgc cagcgttgcg attgtacaga ttgaacagga
     7741 tgttatccca gcagtgcgtc atgttccagt acacattgac cacgacttct ggcagtgcgc
     7801 acagcgccag ccacagggca ccgcgccatc ggcgccgccc gtccgcggta aatgcaaacc
     7861 agccgaacag gccgagaccg tacaccacgg caaaatactt ggatagaaaa gcacacccca
     7921 gccagaaacc cgccagggca taggcgcgcc gggaatcgct gcgtacggca tgcaccatgg
     7981 accagcccga cagaaacacg aacaagatca gtggcgtgtc ggtggtaatc agcacattca
     8041 tccagtcgaa cggccagagc aggaacaggg tcgccgtcca cgctgcccgt tccacccccc
     8101 acggccgcag caaccggtac accagtacgc cgaccgcact cgaaaacagc agcgccggca
     8161 ggcgcaccca ccaggccgcg tgcaccagcg gcaacagcgg cgccagtatc catgccacca
     8221 tcggcggatg gtcgtaatag ccgaaatcca ggtggcgcgc ccagacgatg aaatacgctt
     8281 catcgcccga cagcggcaac accgccgcca atccgagctt gataaggaaa gtcagcgcca
     8341 gaacacgcag aaacagtgat cgtacttgca tgcgctaatt ttaccgcaac ccgggcgatt
     8401 attcgggtta gaatgcgggt tttcatcgta ccgtggagtc gtcatgagtg aacaggaagt
     8461 ccaggccgtt ttcgccatcg aaaaactgta cgtcaaggat ctttcggtcg aagtccccaa
     8521 ttcgccacag atctttctcg agcgcgaaac gccggaaatc ggtgtccagt tgaccacgca
     8581 aagcggcccc atcgctgacg gtgtctatga aaccattgtc aaggcaacgg tcacggcaaa
     8641 actcggcgac aaggtcatgt tcctggtgga agctgcccag gcaggcatct tccgcatcac
     8701 ggatattccg gaagaccaac tcgaaccggt actcggcatc ggctgcccga acatcgtctt
     8761 cccctatctg cgcgagaccg tatcagatct cgtcggccgc gccggctttc cgcccgtact
     8821 gctcaatccg gtcaacttcg aagccctgta catgcagcag aagcaaaccg cgcccgccag
     8881 cccggccagc cactgagaca tcacgcatgc gccgcatcgc gctcctgctg gcatgcctga
     8941 cgaccctgcc tgcacaggcc ctggatttcc gctcggtcgg cacggcagcg gcgatcctgt
     9001 atgacggccc atccaccggc agccagcaac tgttcgtcgt caaccgcggc ttcccgctcg
     9061 aagtcgtcac cacccatggc aaatggggcc gggtgcgcga tccggtcggc aagctcgcat
     9121 ggatcgaact cgattcgctg tctgccacac gcacagtcct cgtgcgcaag gccagcgatg
     9181 cccatctcgc tgccgacacg gcctcgccgg tgtgcgcgca cgtacgggcc ggcgtactgc
     9241 tgccctacct cggcatgagc gctaacggct gggcccgggt gcgcttgccc gatcagcgta
     9301 cggcctacat cacactcgac gcattgtggg gcgcatgagg ctgtgcattc tcggagccgg
     9361 cgcctggggc agtgcgcttg cgatccactt ttcaacgtac catgatgtca tgttatgggc
     9421 gcgggatgca gcgcacgttg ccaccatgcg cagccaacgc accaacacac gctacttgcc
     9481 gggaatcgcc tttcccgacg cactcaggct gacagaagac ctgcgcgcct gtgtcgatca
     9541 tgccgacgca gtgctggtcg ccgtaccgag taccggcttt cgtgcgttgc ttacgcagat
     9601 cgcgccctgg ctgggcatga caccgctcat ctgggcctgc aagggtttcg agccgggcag
     9661 catgctgctg ccgcaccagg ttgcagagca gaccctggat ctggctacac cacggggcgt
     9721 gctgtccggt ccgagttttg ccagcgaagt cgcgcgcgga ctacccactg cgcttaccct
     9781 cgcaagcgac agtgccgcga cacgcgacct cgccacgcaa ctgcatcacc agcatctgcg
     9841 tgtctacacc agcgacgacg tcatcggtgt cgagatcggc ggcgcactga aaaacgtcat
     9901 ggccattgcc gccggcattt ccgacggcct ggcgctcggc cacaatgccc gcgccgcact
     9961 gatcgcccgc ggactggcag aaatcagccg gctgggtatc ctgctcggcg gtcatccaga
    10021 aacattcatg gggctgaccg gtatgggcga cctgatcctc accgcgacca gcgatcagtc
    10081 gcgcaaccgc caggtggggc t
//
LOCUS       DAIANL010000122         4850 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_768719, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000122 DAIANL010000000
VERSION     DAIANL010000122.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768719"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          906 a         1376 c         1577 g          991 t
ORIGIN      
        1 catgtccttc cagcgggaga tgcccagcga gctggtgaac accgtgaagc cgtgttcggc
       61 ggcatacagc gccgcgcgct cgaagcgcat gtcgaaacag gcggtacagc gaatgccccg
      121 ttcgggttcc cactccaggc cgcgcacccg gtcgaaccag ttgtccttgt cgtaatccag
      181 atccacgaac ggaatcccga gcttgctggc gtagcggatg ttctcttcct tgcgcaaacg
      241 gtattcatct tcggggtgga tattgggatt gtagaacagg atcgtgagcg agacgccggt
      301 gcgctggata tcggccatga ccgggccgga gcacggcgcg caacaggaat gcagcagcac
      361 atgttcgctg ccgtcaggcg tgcggatcgg ttcgggagcg tttctggtca tgggcggtgc
      421 cagccagtgg gtcattgtgc gatgatagca cgaagcgtgg tggccattgg tcagcccgac
      481 aggtcagaat ggctcgatgc gcgcctcatg gcggtagatg accagacgat acagcgtttc
      541 gccgacctgg atatgactcg attcacggcg gtcgagctgg atcgtgctgc cgccttgcgc
      601 tgacaggcta cgaaaaccga acagctgatt ttcgatcagc gcgttgagaa tgtcgagaca
      661 ttccgtttct ggcaggctgc aacccgtatt gtcgatgagc gtgcaggttt tgccctgcgc
      721 gttgagggcg ctgtagcgtt tcgcgtcttc acgcaacgga ttgtcgatgt cgaaattggc
      781 cagcggatcg atgtgaggaa atgtggattc catggttagt tgcggctctg tcttaaaaga
      841 tgggtatgag tcattgacgg gattgaattt ttgcaattat agtacggata tggatgcaca
      901 gctcgatgcg ttggaaacca aactgggaaa cttgctggaa cactgcgatg ccttgcgcat
      961 ggagaaccag cgtttgcgcc agcagattgc cgtcgccaat gaccagaaca agcagctgaa
     1021 ggaacgggct gcgctggctt cgttgcggct ggaatccctg ctggtcaaat tacccggagg
     1081 cgattgatgg ctcgcgagca gaaggcgctg gacatttaca tcatggggcg cgaataccgt
     1141 atttcgtgcc cggaatccga ggctgaggcc ctgctgcagg ctgcgcaata tctcgatatc
     1201 aagatgcgcg agatccgcga gcagggcaaa gtgatcggca tcgagcgcat cgcggtgatg
     1261 gcggcactca acatggcgtt cgagatgctg aaccgacccg cgggtctgcc ggttgacatc
     1321 gatgctgcgc agcgtagaat cgaaaccatg gaagcacgga ttgatgaggc gctgcggtca
     1381 cagaccggcc tgttcgacgc cgccgcttcc tgattgtgca acatcgccaa tgttccctgc
     1441 ggtgtttgtc cgggttgcaa tttctttgaa ccaatatgta atgactggtt gcggcaaatg
     1501 ccgggagccg ttgtgcgcgt cacgctgctg atgtgcctga agccccgccg ctgcgacccc
     1561 ttgaaccccc ggttcaagac tcgcaacccg acggcatcag cggggactcc agaatgacag
     1621 ccgagaccgg cgtcactgtc cctgttttat cttccggcaa gcacacgctg cgcggacaat
     1681 tgcgtgcgcg tcgccaaggc ttgagccggc tggcgcgacg tctggccgag cagcgtatcg
     1741 tccggcacgc agtgaacgcc gggctgcact ggcgcgcgcg tcgctttggc gtgtacgcag
     1801 cgcatggtgc cgagctcggt acccgggcgc tcatcgcgcg cctgctcgca ttgggcaaag
     1861 acgtttacct gccggtgctg gcacgcgaac cgggcaaccg ccgcatgtgg tttgcccggg
     1921 tgcgttccga ttcgcgctgg cggcgcaacc gtttcggcat cgctgaaatc gagggtgggg
     1981 agcgtgtgcg tgcgagcgga ctcgacatcc tgtttctgcc gctggtcggg tatgatgccc
     2041 acggctaccg tctgggcatg ggcggagggt tttatgatgc cagcctggcg cacctgcact
     2101 tgctgcgcgt ctggcgtaaa cctctgcgaa tcggggttgc ctattcggtg caatatgtgg
     2161 cgcatgactt gccgcacgaa ccctgggatg taccgctcga tgccatcctg acagaacggg
     2221 ggctgcatta ttttccgttg acggccgctg gggacacccg gcaccgggtg agtgcggcgg
     2281 cacgcaaata gtgccttgac agatgctccg acttgctggt tgtttcaggg ttacgcacac
     2341 aggcgggctg acattccctt gtaggtacag gtaagcggac atccgggtat tgtttctata
     2401 actagttgat tttaagcaat ttagcttgtt gcggaatttt taatcgaatt agcaagatcc
     2461 ttgatggatc gtcatttttc gtgattgcac ctatattacc cacataatta tccacagctt
     2521 gtgtgggtaa ataaaaaacc cgtgccggat gaatggcctg gcccgaattg ctcatcaaaa
     2581 actcaagctt ttgcgcgcgt catgcgcagg ggcatccggg tcgggtagac gcaatttttg
     2641 gtgattttgc gcgcgtgcgt tcacgcaccg ccgtaatgcg gtggtgcctg cacaggcaat
     2701 actgaggagc gttcaggtgc tggcgcgcgg atgccacgcc gggctgagcc ggtgtacggc
     2761 ctcaaccagc gcatggacat tttctggccg tgcttctggc gtgatgccgt gccccaggtt
     2821 gaacacatgg ccgttgccgt tgccgaaact ttcgagcgta cgtgccactt cgcgttcaat
     2881 ggtgctggcg tcggtgagca tgatggccgg atcgagattg ccctgcagtg cgacatgcgc
     2941 acccacacgc cggcgcgctt cgcccaggtc ggtcgtccag tcgagaccga gcgcgtccgc
     3001 gccgctgagg gacatggatt caagccactg gccgccgcct ttggtgaaca gcaccaccgg
     3061 caccgtggcg ccttcgtgct ggcgcgtcag tccggcgatc acttcgcgca tcggtgccag
     3121 cgaaaactgc tgataggcgt catgcgacag cgtgccgccc caggtgtcga atagcatgat
     3181 tgcctgggca ccagcctcaa tctgtgcatt gaggtagtcg gtgacggcgc gcacgttgat
     3241 atcgaggatg tggcgcagca ggtcagggcg ttggtacagc attttcttga cctgactgaa
     3301 gtcggtaccg ccctggcctt cgatcatgta gcaggcaagg gtgaacgggc tgccggaaaa
     3361 cccgatgagg ggcacgctac cgtcgagcgc gcggcggatc tgccgcacgg catcgatcac
     3421 gtagttcagg tgctcggtcg gatcgggggc agccagcgca cggatctccc attcttcgcg
     3481 caacgggcgt tcgaaacgcg ggccttcacc cgcgacgaag tgcaggccga gtcccatggc
     3541 atcaggtacg gtgaggatgt cggaaaacag gattgcggca tcgaggcgga atcgcgccag
     3601 cggctgcaat gtgacttcgg tggccaggtc ggggctcttg cacagcgtga gaaaatcgcc
     3661 ggcacgcgta cgtgtctgat tgtactcagg cagatagcgg ccagcctgcc gcatgatcca
     3721 gatgggggtg cgatcggtgg gctggcgcag cagcgcgcgc aggaagaggt cgttgcgtgg
     3781 caggctcatg aagggttccg tttcctggat cgaagagaaa attgcgcgcg ccgatcctgt
     3841 ggcttaccac aggcccagcg agccgagaat gccttgcgca gcctgtcgtc cttctgcgac
     3901 tgccgttacg acgaggtcgg agccgcgcac catgtcgccg ccggcaaaaa tcttcgggtt
     3961 ggctgtctgg aatgcgtggc ggctaccgcg ggcgatgacg cggccgcttt cgtcagtggt
     4021 gatgccgaat tcggcaaacc atggcgcagg actggggcga aaaccgaaag cgacgatgac
     4081 gcggtcggcg ggtatggttt cctcggagcc ggcgataacg accggggtgc gacgtccgcg
     4141 cgcatccggg gcacccagtt cggtggtgac cacgcgcacg ccctcgacct tgctgttgcc
     4201 gacaatggcg accggctggc ggttccacag gaactctacg ccttcttcca tggcattggt
     4261 gacttcgcgg cgtgaaccgg gcatgttgtc acgatcgcgc cgataggcgc aggtgaccga
     4321 agccgcaccc tggcggatgc tggtgcgcgt gcagtccatg gcggtgtcgc caccaccgag
     4381 cacgacgacg cgctggcccg ccatcgagtc gaaggccgtg ttgtcgtcgg ggtgcatgcg
     4441 gtgacgcacg ttggcgatca ggaacggcag cgcttcgagt acgcccggca ggtcttctcc
     4501 cggaaagccg ccctgcatgt agcggtaggt gcccatgccg aggaagacgg catcgtattc
     4561 gtcgagcaac tggcgcatgc gtgcctgatc aacctcgacg cgcaaatgaa agcggatgcc
     4621 catgccttca aaaatttcgc gtcgcatgcg catgacgctt ttttccatct tgaattcggg
     4681 gatgccaaag gtgagcaggc caccgatttc ctcatagcgg tcgaatacgt cggcctgaac
     4741 accgttgcgt gccaggacgt cggcacaggc caggccggcg gggcctgcgc cgatcacggc
     4801 cacccgtttc ccgctggcca ccacgcggct catgtcggga cgccagccag
//
LOCUS       DAIANL010000123        25947 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2194232, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000123 DAIANL010000000
VERSION     DAIANL010000123.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 25947)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25947)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25947
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2194232"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5489 a         8142 c         7180 g         5136 t
ORIGIN      
        1 cctgatgccg cacaatgcca agctgcatac ctatctggcc gacatggaac cggtgatcgc
       61 catgcagccc gatgcgctga tcatggctga ccccggcctg attctcatgg tgcgtgaacg
      121 ctggccggag atcccggtcc acctgtcggt acaggccaac acggtcaatt ttgccaacgt
      181 gcgtttctgg caatcggtgg ggctgacccg cgtcatcctg tcccgggaac tgtcgctcga
      241 cgaagttgcc gaaatccgcc agcgctgtcc cgacatggaa ctggaagtct tcgtgcacgg
      301 tgcgctgtgc atcgcctact ccggtcgatg cctgctgtca ggctacttca atcaccgcga
      361 ccccaaccag ggcacctgca ccaattcctg tcgctgggat taccgcgtac acgaagcgcc
      421 tggcgagtct gccatcgcga acgaaacgcc gcacccgctg gccgagcgca cctggctact
      481 cgaagaaagc aggcgtccgg acgaacgcat gccgattctg gaagacgaac atggcaccta
      541 catcctcaac tccaaggatc tgcgcgccat cgaacacgtg caacggctgg tcgaaatcgg
      601 catcgactca ttcaagatcg agggccgcac caaatccggc tattacgtcg cacgtgccgt
      661 gcaaagctac cgccaggcga tcgacgatgc cgtgcacggc cggccgttcg acctccgtct
      721 gctggcaggg ctggaaggac tggccaaccg tggctacacc gatggcttct accagcgcca
      781 tcacacgcac gaatatcaga actacctgcg cggacattca gagagtgcgc gcagccagta
      841 tgtgggcgac attacaggat acggaggtga tgccggctgg gcggaagtgg cggtgaaaaa
      901 ccgttttgcc gtcggcgacc ggctggaaat catccacccg caaggcaatc gcatcatcga
      961 actgcatgcc atgcgcgatc tggatgacca gccggtacgg gtggcgcccg gcgatggtca
     1021 tcgtgtgcgg attgacttgc cggagggtct ggagggctcg atgctgagcc ggtttctcct
     1081 cacggacgcc gagtgattcc tgagcgtccg cgacagcgac ctgtgatgca atgagcccag
     1141 gctccgcggc aagcgcccgc cgcgggtgcg actgcggcgc agcagggctc ttagccgccc
     1201 ggaccctgtt cctcaagcac gagatcggcc ttgcctgcgg ccagcagcgt attttccatc
     1261 aggatggcca ccgtcatggg accgacgcca ccgggcaccg gcgtgatcca cgccgcacgc
     1321 tgggccgcga ccgcgaaatc gacgtcacca cacagacggc catccggcag ccggttgatg
     1381 cccacatcga tcaccgtggc accttcacgg atccattcgc catgcaccag ccccggtcga
     1441 cccaccgcag aaatcacgat ttcggcacgg gccacctgct cggccagatc gcgggtgaag
     1501 cggtggcacg tggtcaccgt gcagcccgcg agcagcaatt cgaggcccat tgggcggccg
     1561 acgtgattcg acgcgccgat gaccaccgca tgccggccct tgagatcatc gtgcgccagt
     1621 gcaagcagac gcatcacgcc ataaggcgtg cacgagcgca gatcgggcat gcgtaccgcg
     1681 agccggccaa tgttgcatgg atggaatccg tccacgtcct tttgcgggtt gatacgttcg
     1741 atcacggttt ccggatcgat gtggcgcggc agtggcaact gcaccagaat gccatgcacc
     1801 tgactgttgt ggttgagctc accaatcagt gccagcaatt cggcctgact cgtgctttcg
     1861 ggcaggtcat agaaaatcga acggataccc acctgttcgc aagcgttgcg cttgttgcgc
     1921 acgtataccg cagaagccgg atccgaaccg accaggatca cggccagggc cggcactgcc
     1981 agccccccct ccagcctgat cgccacctgc tcgcgcaagc cggcaagaat ttcagtcgag
     2041 agcttttttc cgtccaggat cgcggcaggc atgatgttcc gtggttatgc aggcaaaagc
     2101 gtatcatcgc attccattct ccgctgttca atccctgcca cgcaaaattc gcgcggattg
     2161 cgcctgtcac aacctgccaa agccaaccga cgacacgatg cgcaacatgt cccgagcgga
     2221 tcaggctgcc cgcatcttga ctaaaccctt ccagcaaggt agagtctcgc tttctcgggg
     2281 cgtagcgtag tctggtagcg cgcctggttt gggaccagga tgtcgggagt tcgaatctct
     2341 ccgccccgac caggattcag gcctggccgc catgtgcggc ctgccgcccg gagcgccgtg
     2401 acctgcccgg tcacagcgct ccgctcccag cctgttgctg cggcggttca tgagctgccc
     2461 gtagctcaac cggatagagc accagccttc taagctgggg gttacaggtt cgattcctgt
     2521 cgggcaggcc agttcaagtt caggaaacaa ccggctgtgc aagccggttg atccaatggt
     2581 ggatgtagct cagttggtag agtcccggat tgtgattccg gtcgtcgtgg gttcgagtcc
     2641 catcatccac cccatcacca tgcaaccggt tgatttgatt ggattgcaca gcataatcaa
     2701 gggtttgtaa cacatgagtg caactcaaat tgtaactcag cccgatatgc agcatacaat
     2761 cttgcgcggt tccacctact ggttttcccg gcgagttccg cttccgacag gcactgcttt
     2821 gacaattccg ggccacccaa ccccacgccg catcggcata aatggtctgg tcaggttttc
     2881 cctcgatact ggcaatcccc gcgaggccgc ccgagctgct ggagttgcca ttgatgacct
     2941 attggccact cgccgagccc ccctgccaaa gccaaaacac cacccaacaa cgccagcgcc
     3001 tcgctagtga atgttacagc tggtggcgct gaccgattgc gagctgtttt agctgaggca
     3061 tctcgttcac cggcatgacg ctgccattcc gggcaaacac atcgaccaac cagctccata
     3121 tctgatcggc aatttcaggg caaagaagcc tcggtttctt caacagcaag cggatgttat
     3181 ggcctgcgcc gtacagtacg gcatgcagcg catcatccag cgcacctttg aggggattgc
     3241 gggcaagccg tccctgatct agtgggcgcc aaaaattgaa accccggcag ttgaggcggc
     3301 cttcgctaaa tcagcatcaa gcgttgccag tggcatgcca gtccgcagcg acagttccaa
     3361 ataagctgca tcataagccg acagtttgta gcgacgggcg aggttcaatg tatcgctcaa
     3421 ggcatgtgcc gctgtgtctt gatcgactga aatgttaaga cgcccgagga cagtaacgta
     3481 gcgctgcgac tcggcttcgg tcacaactcc cctggattcg agcctggcaa ccgcattggc
     3541 aatctcgagc gccaacagtg atggcacgac agcttgtgaa gctttaaggc ccatcagtat
     3601 ctcggcagcg taatccgcgc ctgtcgggtt ggtttgaggt gccagccata gcaatgccac
     3661 cgatgcatcg agaacaaaat tcactcgcgc ccctcgtcaa tcagcgcttt cagatcaata
     3721 cagacgccag atttatgagc tttcatgaag gagagcatgt catcaacggc taccacggca
     3781 tccaactttt tgcttccttg cactggcaca aggtcggcca cggcctcacc tcgctgggtg
     3841 atggtatagc gattaccggc ctggatacct cgcaacaact ccggcaattt tgtctttgct
     3901 tcatatgacc caaccttgat attcatatcg gctcgcctca tcttttacgt atagaccagt
     3961 ttatagaccg gtctataaaa gtcaaggttg aattgagcca accacatgtt gcaagagcac
     4021 cggcttcgag gatcgataag tcttgccgcc cgctcaaatg gtatctcagt ccggaaacac
     4081 aaccttgccg ccatcctgtc cagtacgcaa cgtcattatc ctgcttgcat ctgatttagc
     4141 aatggttttt gaatggcctg ccaaccggtc agaccatacg tcggcaagcg cacaggcgat
     4201 gcagtccgga tggctacggc ccggcactgc acggtgcggt catggacaga cgcggcaggg
     4261 attcgggatc tcatcaggac attcggctcc ataaaaccag tattgcattc gatcggattt
     4321 tgaggtaccg tttaaacatg cataaattat gcatgtttaa actttcaacc cgaccaagga
     4381 gactggcgat gcaacagaca ttcaccaaag ccatggctcg caacatcttt ctcggaggcg
     4441 ccgcgttttt tctcgtcatt tatctggtcc tgaccgtcga ttcctggcgt cagatcccga
     4501 tacgcgacaa cgccgcagag atgtcgccag cagccgtacg cggcaagtac ctgatggaca
     4561 gcaacaactg tatcggatgc cacaccatca acggagaagg cgcctattac gctccagaac
     4621 tgggcaatgt gtacacccgc cgcggaccgg atttcatcaa ggcctggatc aaggcgcaac
     4681 cgatcggcgg acatcggcag atgcctcatt tcaatttcag cgatcgtgat ctcgatgaca
     4741 tcacggccta cctgcagtgg gtctctcaca tcaacaccaa ccactggccg cccaacctcc
     4801 agggctgatc ccttgctgat cccgtcaacc ctggcaccac aaggagcaat catgaatttc
     4861 acatcgcaac gggtggccaa accctacttt atcggcgccc tggccctgtt cgcaggccaa
     4921 atcatttttg gcctgatcgc aggcatgcaa tatctggacg gaagtttcct gttccccgaa
     4981 tttccgttca gcgtcatccg taccgtccac accaacctgc tgatcgtctg gctgctgatg
     5041 gcctttatgg gtgccgccta ctacattgtt ccggaggagg cgcagaccga gctgtacagc
     5101 ccaagactcg ccttgatcac cttctgggta ttcctgatcg ccggcgcatt gaccatcctc
     5161 gcttacctgt tcgtgcctta ttcgcagctg gccgccatga ccggtaatgc gatcctgccg
     5221 accatggggc gcggctatct ggaacaacca ctgcccacca agatcggcat cgtgctcgtg
     5281 gcactgtcct tgctgttcaa tgtcagcatg accctgatca agggccgcaa gacttcgctc
     5341 aacctcgtgc tcgtggccgg tctgtggggt ctggcgctgc tgttcctgcc cgccttctac
     5401 tttccggtcg atacggtcaa agccagctac agctggtggt gggtggtgca tggctgggtc
     5461 gaaggcgact gggaactgat cctgagcgca ctgcttggtt tcattctcgt caaaatgacc
     5521 ggcgtcgacc gcgagatcgt ggaaaaatgg atgtacatca tcatcgccat gacgctgatt
     5581 tccggcatca tcggcatcgg ccaccattac tactacacgg gtgcgccgga atactggatc
     5641 tggctcggtt ccatcttcag cgccatggag ccggtgccct tcctgatgct ggtcggcttt
     5701 gccgtgcgca tgctgatcaa gcgccggcag gagtacccca accatgctgc catgctgtgg
     5761 gcaatcggca ctccggtgat ggcgttcctg ggcgccgggt tctgggggtt catgatcacc
     5821 ctcgcgccgg tacagtatta cacccatggc accaatttca ccgcagcaca cggacacctc
     5881 gccttcttcg gtgcctatgc catggtgtca ctgtccatca tttcttatgc catgccgcat
     5941 ctccgcggac gggtggccaa cagtccgagc gctcaacgct gggaaatgac cggcttctgg
     6001 atcatggtga tcagcatgtt cgtgatcgca ctggctctga cgggtgccgg gatcgtcacg
     6061 gtattcatgc aacgcatggg ggatcatccg atcagcttca tgggcgcgca ggaccaggag
     6121 cgcgtgtttt tctggatacg ggaagtggcc ggattcatct tcttcggcgg attgctgacc
     6181 ttcctcacca gcttcttcgt gggtaacaac gagtctcttg cctcaccaga ggctcagata
     6241 cccaccagac gctgatccat gcccggggcg aacgcatgca ctgcgtgcgc cctccccgcc
     6301 gccggcaaga cggcccgctc ctccgaccga cttgccggca ctaccgcttc aacggatggc
     6361 cactcatgga cacagacaac acccttcatg ccaacacagc agaacaagac cctctgcccg
     6421 gggatttcgc catctgggtg ttcatttttg ccgaactgac cctgtttggc gtcctgttca
     6481 cggcctatgc gttcgcccgt gccagtcata tccagctttt cgatgccgag cagcgactgc
     6541 tggaccggca gacggccctg atcaacacgc tgctactgat caccagcagc tatgccgtgg
     6601 tgcgggccgt gctggcaatc cgtgccgacc agcgtcgcaa ctgcgtccgc tggctgctgg
     6661 cagcaatggg gttgggcggg ggatttctgg tcctcaaggt cagggaattc gcccatgatg
     6721 caacccttgg catcaccatt tcgcacaatc tgttcgacat gttctacctg agtctgacct
     6781 tctttcattt catgcacgtc attctgggca tggtaatcct ggctgcggtg gcctttcggg
     6841 cccatggcgg gcactacagc gccagttccc atgcaggggt cgaaaccggc ggtgcctact
     6901 ggcacatggt cgacctgcta tgggtcatcc tgttcgctct cgtttacgtc attcactagg
     6961 agctcatcat gagcgacgca cccactgtca ggcacgacat ccagcgcgta accctggtct
     7021 ggggactgct ggtcttcctg acgctgtgca acttttatct gagcgcacga ctgaacggag
     7081 ttttgctgat ggcgacggtt cttgccctgg ccatcgtcaa ggcggggtgg atcgcatttg
     7141 actacatggg cctgcgccac accagactgg cctggcgcat ggtcatgagt ctgtggcttg
     7201 cgctggtagc cacgggaatc gccattgcct atctttttcc tgcctgaacg gagaactccc
     7261 atgctttcac tgacagattc cccctcccgt cttccggatt acctcgcatc cggccaggag
     7321 aaagaattgt tcgaaatggc ctggcgcgac cggctgccgg tactgatcaa aggaccgacc
     7381 ggttgcggca agacacggtt cgtggaacac atggctgccc gcctgggtcg cccgctgttt
     7441 accgtttcct gtcatgacga cctgaccgcc gcagatctgg tcggccgcca cctgatcaat
     7501 gccgacggga cctactgggt cgatggcccg ctcacgcgcg ccgtgcggca aggcggcatc
     7561 tgctatctgg acgaaatcgt ggaagcccgc aaggacacga ccgtcattct gcatgccctg
     7621 tctgacgacc ggcgtctgct gccgatcgaa cgcaccggag aactgttgca ggcgccagcg
     7681 gaattcatgc tggtcgtctc atacaacccc ggctaccagc atgtgctcaa gggattgaaa
     7741 ccaagcacgc gccagcgctt catctcgctg aatttcggct ttccggaacc ggaacgcgag
     7801 cggcagatca tcgtcgggga atcttcgctg cctgccgaca aggctcagga tctggtccgg
     7861 ctcggccagg cattgcggca aaccgcgccg catgatctgg acgaagcccc gggcgcgcgc
     7921 ctgctggtca atgccgcacg gctcatgcag cagggctgcc cgcccggcca ggcctgtcag
     7981 gttgccatcg tggaatgtct gaccgatgat gcagaaaccg tccagggcct gatgcaagtg
     8041 gttcacgcca tcatgggaga ataaccatca tggaagagca gatcggcatc tggtggcacc
     8101 ggctggtcac ccggctggcc gaagaccgga ccactccggc ccgtgtcagt ctgtctgaca
     8161 tgcggcgtga accggggatt ctgttccgtg cattcggcgg cgctcatggc ctcaaactaa
     8221 gcccggctcc cctcggacga acgaaacgaa cccgattcgt ccagcggctc gccggaaccg
     8281 gcggacgcct gccactggcc acattcaacg cacagtcgct ctgtctgccg gaatacgtcg
     8341 acgtatatgc cacccctgcg ctgcatcggg atctgtatcg ctggtgggcc atgctggccg
     8401 gccagttgcc tggtctgcag ggcaacgggc tggttgcctg ccagcacggc acacgcacgc
     8461 tgatcgcccg ctacccgggg atcgacttcc tgtaccggcg tctggtccag gcagaaatcg
     8521 cgcgtcgggg tccactggat cggttgccgg atgccagaca acccgtcgaa cgggctctgc
     8581 gccaggctct gctgaacccg ggtagcgtcg ccaccctgcc gcccgggcgt ctgccccaac
     8641 ctgtactgct ctggccgatc caggccgacc aacccacacc ggatgccacg accggcgacg
     8701 aagaaccgga tgcagacccc gttcagccgg tggtcgggca ggacgacaaa cggcgccagg
     8761 cccagcgctc tgagcagact gcacgcagcg gtggtttatt tctgccccgg ccggaatcca
     8821 ttttcagctg gaccgaatat atcaatgtgg aacatccgat agaggaaaac gaggaagatg
     8881 atctggcccg cgcagccgac gatctggagg tcctgcatct caaccgttct gcccagcgcg
     8941 tcagccggaa attgcgcatg aatctggaaa ccggtgaacg cgccggaacg gcgacgcctg
     9001 ctccgcccgc gctgcatctg cctgaatgga acttcagaac caacatgctg cagccagatc
     9061 actgttcggt gcgaattctg ccccacgctc cggccgcgct cggctgtgac ttgccggaac
     9121 gtctgcggac cggccacaga aaactgctgg cgcgcctctc ggctctgctg ccggagaacc
     9181 agacgctgcg cgggcaggcc gatggcagca gcatcgacct cgacgcatgc atccgggcac
     9241 gcaccggcga gcaccgccag agcgaattca tgtacctgaa cccacgccgc aaggaacgcg
     9301 acctgtcctg cctgctgctg gccgattttt ccctctcgac ggaagccgca ctcgaccagc
     9361 agacacgggt gctggacgtt ctgcgcgaca gcctgctgct gtttgccgaa accctgtcca
     9421 cgacacgcga ccgggtggcc atcctgggtt tcaattcccg cggccggcat caagtgcgca
     9481 tcagcgaaat caagccgttt ggcgaacgtt acggcagcgc aacccgacag cggattctcg
     9541 acatgcaacc cgcctattac acacgcatgg gagctgcagt gcgttatgcc agtctgatgc
     9601 tgggaaagga acgcacacgc aaacgcctgt tgatcctgct gaccgacggc aaaccgaacg
     9661 acaacgacca ttatgaaggg cgctacggcg tcgaagacac gcgcaaggca ctgagcgaat
     9721 gccggcgcca gggcttgctg cccttttgcg tcaccatcga ccaggaaggc gaaacctatc
     9781 tgccttacat ctttggtcag cagggctttg tcatcgtgaa aaatccgcgc gagctgccgc
     9841 agaggctgac ccggttgtac gtgcagctga cccgttcgat gcactgatcc gcggccccgg
     9901 ccgcgccaac cggatggcgt agtcgggatc tgtcactcag acctgccggt ttgcaaaatc
     9961 agtccgactt ttcagccgct tccacaggct ggtgcgcgac atccccagcc tgcgcgctgc
    10021 ttcggccttg ttgcctgccg tctcggccag aacccgttcg atcagagcca tctcattctg
    10081 tccggagccc gggaagcgat aataggatcg caacgccagc ccgatacccg gcattgccgc
    10141 gccattctcc gtcgcagcct ggccgatgtc cggcggcaga tgttcgggca gaatctcccg
    10201 gccatccgag ttcaccagcg catactccag agcattgaac agctgcctca catttcccgg
    10261 ccagttgtaa ttctccagaa gagccagcgt ttcacgcgaa atcgccagat ccctgcggcc
    10321 gtatttgctg gcaagattat tgagcaggct gtaggcaatc agcgagatgt cttcagtccg
    10381 ttcgcgcagt gccggtacct gcatgggcag gacacacaac cggtaataca ggtcttcgcg
    10441 gaattcaccg cggcggacca tctccgccag attgcgatgc gtggctgcga tcacccgcac
    10501 atcaaccgga cgtggctgat tctctcccag ccgcacgatt tctctctcct gcagcacgcg
    10561 caggagcttg acctgcgtgg ccagcgggat ctcgccaatc tcgtcgagca gcatcgtacc
    10621 gccatcggcc gcctcaaaac gaccgattct ggttgatgtg gcacccgaaa aagcgccttt
    10681 ggcgtagccg aacaattcgg attcaagcag atgctccggt aatgccgcgc aatgcaccgg
    10741 cacatagggt ccctggctgc gcgtagagtg atcatgcagc gcccgggcca ccatttcctt
    10801 tccggtgccg ctctccccgc tgatcaacac gctcgcatca cttttggccg tccgcatgat
    10861 ctggttatac agtttttgca tgacctggct atggccgatg atcccaccga actgcaaccg
    10921 ttccgcagcg tattcctcca gctcgacctc gtgcgaccga tcgcgcaaaa tgattgcact
    10981 gtggcactgg ttgccctcgg tcgaaggcaa cagcatggcc cacatgcgca gattcaccgt
    11041 gctgccgtcc ggccgtacca gcagcaggtc cctcacgtgg ctgtcattcc ccggaaacgg
    11101 cgccagtaca cagtggccgc ggcgctcgca ggcgcgaccg cacgccgttt ccctgaaaag
    11161 atgcaggcac agctggccga ccatggcccc tcggtcccaa ccgaccattt ccgccgctga
    11221 ctggttcgcc atcatcacgt gacgggtccg gtccagaaaa agcacaccct cttcaagact
    11281 gtcaagaaac cgatccagca tcacatccgt gatcatcatg cagcacctcc gttcaatgct
    11341 gaccgttaac aaacgttcaa tgtgaacgca ataaacagtt gcaacagttc gttcataaaa
    11401 aattcacaat tctttcatat tgttactttc tggcacgatc ctggctaaag caaggaacag
    11461 gttgtcatcg atcaccggtc acaactttca gccctcacag aaaggaaaaa caatgaaact
    11521 tcatctcacg gcaatttcag cactcatcgg tctcggtctc cttgcgtcgc aagcaagctt
    11581 tggcaaggat ttgtcctgcg cggactggag cttcgacaat ccgcactgcc cggcttatat
    11641 cacgcctgcc catgcgacct cggctcagcc ggccaacgcc ccgcaacctg cccagtgcaa
    11701 tgactggagc ttcaatgatc cgcattgcgc cgccttcatc agcccaccta accggcttgc
    11761 cgcagccaac cccgttgctg cgggcaatcc ggctggggcc atgtgcgccg actggagttt
    11821 caacgaccca tcctgtcctg cttacatcag gcactgaggc catgccagcg gatcgtacga
    11881 tccgtgggta cattgccgat accgggtatc cgaatcattt tttttgggct ataatacgca
    11941 ttaataatgc atgttatagc cccggaaatc aatcatgctg actgaaacca accgaaatct
    12001 ggtcaaagcg accgcacccg tccttcagga gcacggcgtc gcgctgaccc gccattttta
    12061 cgcgcgcatg ttcacccaca acccggaact gcgtgaactg ttcaaccagg ggcaccagca
    12121 caggggcgat cagcagaagg ccctggccat ggctgttgcg gcctatgcca cgcatatcga
    12181 agatccatcg gtactgatgc ccgtgctgga aaggatcgcc gccaaacatg tcagcctcgg
    12241 tatccgcaag gaacattacg cgatcgtggg ccgccatctg ctcgcctcca ttcgcgaagt
    12301 gctcggtacg gccgccaccc aggaactgat cgatgcttgg gccgcagcct atacccagct
    12361 ggcagaactg ctgaccggga ttgaacagaa cctgtatgac catgccgtcg cccaggatgg
    12421 cggctggaca ggatggcgaa cattccgtgt ccaccgcaaa tcagcagaaa gcgacgaaat
    12481 catctcgttc gagatcgttc ccgctgacgg cggaccggtt cccatctacc gccccggaca
    12541 atacatttcg gtgcgcctca tcgttgcgaa cctgggttac cgccaggcac gtcagtacag
    12601 cctgtcggat gcgccaggaa aaaacggttt ccgtatctcc gtcaagcgcg aacgcgcgca
    12661 ggatcagcag ccggatggaa tggtctccac ttgcctgcat gaccgcgtca acgaaggcga
    12721 cctgctggag gttgccccgc cggctggcga tttttatctc gatgaagaca gtcgggatcc
    12781 ggtggtgctc atcagtggtg gtgtaggcat cacaccgatg atggccatgc tggaacacct
    12841 ggccgccacg aaccccgagc gcacagtgcg ctttcttcat gcaacccgcc acgcaggggc
    12901 gcaggcattc ggattgcgcg tcgaagaact ggtggaccgc atgtctgatg cccagtcatg
    12961 gatcgttcat gaatccggac ctgccccgga cgccgtccgc tgtcatgcca ccggacgtct
    13021 cgaactgtcc ggcttgaccg acagtgcctt gttgccaaag aatgccgatt attacatttg
    13081 tggcccggat ggtttcatgc gggcgcagat ccatgcgctg cgcgaactcg gcactcccga
    13141 acgccggatt cacgcagaag catttggcac aggaggtgtg gcggcctgaa gaggtcggaa
    13201 tcgtcagact cttcgaccgg gacattcctg tacatcgtca ccatgcgagg gaatgatgca
    13261 actgacacga tttaccgatc tcggcctgcg tgttctgatg tttcttggcg tttcggaacc
    13321 cggaaaaatc aggaccattg cagaaattgc agaaaagttt ggcgctgcac cgaatcacac
    13381 gaccaaggtc gttcagttca tggggcaaca gggatggctg gacaatattc gcggaaaagg
    13441 ggggggcatc catttggcca ggccggtaga gagttaccgg ctcggcgaca tcgtgcggat
    13501 cctggagcgg aaagacgaac tgatcgaatg tggccggccg ctgtgcgccc tggccggcgg
    13561 ctgtgcactg aaacagttgc tggataacgc aaccgatacg ttctacacgg aactcaaccg
    13621 gcacacgctg gcggatgcca ttgcgggaac aacccgccag accatcatca gattgcagaa
    13681 gtaagcacag gttacatacc ggctgtccgc aaccggaaaa gacaggacgc caggcgcgcc
    13741 agaatttttt acaacagctc tcataaaaaa aacagaactg ccggcccaag tcgacatgat
    13801 caatctatac cgctattcaa aaatactgac tgcgcggcaa ttcgtgctgt tcaatctggt
    13861 gctcgggatt gaacacttcc tcgtcttgct caacgctggc gcctatctgc caatgctgcc
    13921 ctatgtgacc ggcaccatgg atgagccgct tgcctacgtc gtctgggggc agagcaatta
    13981 ctttgccgcc atgggggcct catttctgat cgcacgtccg ctgatgaagc gcttcggccc
    14041 caagaacgtc acgctctttg ccggaatcct cttttccatg acctgtctcg ccgccctgct
    14101 ttccgtccat cagtttgtgc tctacacggc agtcagaatg gtacagggct atgccgcggg
    14161 actgagcctg attccttctt ttttcctgtt gctggagtac taccgggaca acaagcagca
    14221 tgtcgccacc tcgctgtggg ggctggcgct gttcgttcca ttttccgtcg ggccggtgtt
    14281 cggcggctgg ctggcctaca cactgggaga ctggcgcctg ctgttcgtca tttcgttttt
    14341 cgtttctctg gccatgacaa cgattctctg ggcccttctc gccgactggg aagatgaaac
    14401 cagccctcat gtttccctgc gttcgcccgt gcgcctgtcc atcatcctgc tggctgccgc
    14461 cacggtactg cagcaatatt tcaatgtcgg tctgctgagc gatctgtcca gccgtttcgg
    14521 gcaattctgg tggctgttct tcgcgtttgt tttcctgatc tggctgttct ggttcgaaaa
    14581 ttcgcgatcg aaaacacccc tggtacattt ctccctgttc ggccggtcca atttcggcag
    14641 cggcatgctg ctgctgtgcc tggcattcat ggccgtccag ggcggcattg tccagtacat
    14701 catccgcatg cagaccgtcg aaggctatac ggcctggcat gtcggcctgc tgttcctgcc
    14761 ggttttcctc ttctccaagc cacttagcgt gctggcgcag aaaagcatca ggaaaggaac
    14821 cgacccgcgg attttcgcct tcatttcgct ctgcatgctg gcagcaagct tccgctggat
    14881 gtcgacttac atccggccgg caacctggga gacgctgctg gctccgcaat tcctgctggg
    14941 tgccggactc ggcctgctgt tcgccccgat gacggccatt tcactggccc acgtgccgaa
    15001 aaacgaccaa ctccatggtc tcgaccttct caataccgca agaaatcttg cagccggtct
    15061 ggccatcact ttttctgata tcggttggga ccgcctgacc gaactcgaag ccaatcgggt
    15121 aatttcaccc gacacctctg atgcggcgcg catccttcag tacaccggct cgccgcacct
    15181 gatccacgaa caactcgtca ggcaggcttc cctgttgacg ttcaacgatc tgtactggtt
    15241 gctttccaca gccctgttcg gactggcgtg ggcagtctgg ttactgcaac cggtagtcgt
    15301 tcgcaatccg gatatggtga ttctggagaa ccttggggaa gaaccttgaa aactcgatac
    15361 cttctcgtga gtctgctgct tgccggatgt gctccggtgc tccgcagcgg tgctccgctg
    15421 accgaccagg tcgcgtttcc acccgaaatg aagcagacgg ccaaaggact gacgcaaagc
    15481 accgacaacg cactaccaga aaaatggtgg cagcgctttg gtgaccacaa cctcgacaat
    15541 gcggtcgaaa cggccctgcg cagcaatcct tcgctgtccg ctgcccgcga cagggtcgcc
    15601 agagcgcaag aagaagtgtc gaatgccgaa tccttgcgct atgcgcatgt caatggcgtt
    15661 gccacaatcg cccaccagga actgtcaaaa accggcaacc atgacattta caatggaaaa
    15721 acttcgacca ttgccacagt attgcccttc gacctgaact acaacctcga cctgtggggc
    15781 cgcaacgcag aaatcatcgc cgccacccag gcttcagaag cgggaatccg tgcccgttac
    15841 cggctgacgg cattgatgct cagtgcttcc gtcatccaga cctatttcgc tctggaagcc
    15901 tccaaccatc tcatcgcatt gcagaacagc accattgccc tgctgaaaga ccggctgaat
    15961 ctcgaagcag ccggttacag cgccggcatc aacacggttc gcgatgatct cgctgccgac
    16021 gcaaccctgc atgcggaaag ggaaaaactc gctctgctgc aacaacagaa tgacgccctg
    16081 aaatacgccc tgctggcctt gcttggaaaa gcgccgggag acaacgtggc tgtttcgccg
    16141 gaacttccaa tgcccgatca tttcagcatt ccgggtcacc tcggcatcaa tactctggcc
    16201 aaccgaccgg acgtccaggc cgccatgtgg aacgtcgaaa aacagaagca tctggagcgg
    16261 gtcgcacaaa aagcgttcta tcccgacatc aacctgtttg ccctggctgg gttcaacagc
    16321 ttcatcggtc tgcacgacct gttccagcca gagagcggaa cctacgccta tggaccggcc
    16381 atcaacctgc ccattttcga aggcggcgca ctggaaagcc ggttcaatgc ccaggaagac
    16441 gcctacgatg ccgctgtgca tgaatacaac cagagcgtac tgaacgcggc acggcaggtc
    16501 gcgggtgcca tttcagggct ggaacacaac cgtgtccgcc tggatgaaga aaccgacacc
    16561 ctgaaactga aaatttccag tgcctccgtc gctgaagcag gattcagttc gggagtcacc
    16621 gggaaactgc cctggattgt ttccgcagtc gaggtcaatc gcgagcagat gcagcatctt
    16681 cacaaaaatc tggactggtt aaacagcatg actattgtcg ccacgaccct tggcggagga
    16741 tttggaaagt aaccttatga agcataataa agcattccga tcgagcatac gtcaccaccg
    16801 cctcaggggg gcgctggttt tcatattgat cctcgccgcc gcggcggttt catggtggta
    16861 tctgtttcag cggacagaag tggacagcga cgatgcctat gtcgccggaa acatcgtgcc
    16921 cgtctatgcg ctggcccccg gaatcgtgtc gaagctgggc gtcgacaaca gcatgccggt
    16981 cgtgaaaggg cagtggctgc ttgacgaaga gaagaatgtc agtcaggcac gcctggacaa
    17041 ggccaccgcc gatctggccg aggccgtcag gaagaccaga agcctgtttg ccaaagtgca
    17101 ggaacagcaa gccgatctgg cagccctggc cacggaccgg aacaatctgg ttcaggatct
    17161 gggccgctac cttcaggccg aggccgccgg agcggtgccg gtgcagaaaa tctctgacac
    17221 acgggccgag atagccgttc tcgaccacaa gatctcggcc ggtcaggcgc atctgaacaa
    17281 tgccagcgca ctggtttccg gtacatcggt cgacaccaac ccgctcgtgc tgcaagcccg
    17341 cgccgaattc gtcacggaat acatcaacga gcaccgcgcc agcctgtatt cgccgatcga
    17401 tggctatatt gccaaccggc gggtccaggc aggcgaaatg ctgaaagccg gacaatacct
    17461 catgtctgtt gtgccgctcg atgaactctg gatcaacgcc aacctgaaag aaacccggct
    17521 ggcccggatc aggaccggcg aaacggtgca gattctggcc catgcctatg gcgacgacgt
    17581 cgtttaccat ggccagatca tcggcatgga tccgtccggc ggcagtacgt tcagcctgtt
    17641 tccgcctaac aacgccacgg gcaactatat ccacatcgtc gagcgcatcc cggtcaggat
    17701 cagcctgtcg cctgccgaat tgcgcgccca tcccttgcgg ccaggcatgt cggtctcggt
    17761 ccggatcagt acggagaact acgccagctt cgcaccgtta tcgacccggg tcagcgcaac
    17821 aggaaaaacc tattcgaccg atctgtacga caccgaaatg caggcggcgc aaaaggcagc
    17881 gcagagcatc atcgacggca accagaacgg ccgctgatct ggcccggagg tcgtctcctc
    17941 ccgcaacgcg atgatcctga tgcaaggatc acgttgccgg aggcgcgacc gttcaggaat
    18001 ccaccatcag tctgacctgc atttttggtg gttcctgaaa tacgcacagc acacgaatga
    18061 accgccatac cacggagcgg attgtgaatg gacggacaca tacaacgttc agcggtgatc
    18121 ggggtctggg ttgcgctgaa aacggtcctg tgtgttgccc tccggtctct gctgacgtcc
    18181 gccgcaccgg actcatcatg caaccgttac ccgcgcgcga ttgcaatcat cttgaaaacc
    18241 tgtgaaaaac gaaataatct gacgttttac atcggataat ccgatacatg gacactgaac
    18301 aggcacgcac cttcctgcac gtcgtcgcgg taggcaattt ccagggagca gcgcaaaagc
    18361 tgcatgtgac gcagtccacc gtcagcgcac gcatccagac actggaaaac cggctcggcg
    18421 ccaaactgtt tcgccgcggc aagaacggcg cagagctcac cgcagccggc caacgcttcc
    18481 tgcgccacgc ccagacgctg gtgcgcacgg tggagatcgc ccgccaggat gtcggcctgc
    18541 cggaaggcta ttccgccgga cttacgctgg caggacgcat tgcattgtgg gaaggattcc
    18601 tgccgaactg ggtggcgtgg atgcgttcgg tagcgccaac catttcgcta cggctggaaa
    18661 tcggtttcga agccgacatc atgcaaggca tcatccacaa caccatcgac atcggcgtga
    18721 tgtatacccc ggaagcccgg ccgggactgg cggtagaacg gctgttcgat gaaacgctgg
    18781 tgctggtgtc caccgatccg cactgccact ggccagaccc gggttacgta catgtcaatt
    18841 gggggccgga atttttcagc cagttctcga tgcaatttcc cgaccacccg ccgccggcgc
    18901 tttatgccaa tatcggatgg ctgggcatcc agcacctgac caccagtggc ggctcgggct
    18961 actttccgtt acgcatggcg cgcgaggcat tacgggaagg ccgactgcac ctggtcggcg
    19021 gcggcacacc atcgttccgc ctgccggtgt acatggtctt tccgctctcc cgtagcgaca
    19081 cgtttctcga acaggcgctg gacggattgc gtcgccttgg ccgcaaagag cgacgcggca
    19141 aaccgcccgc aacccgaacc cgaccgtaaa tgcgatccgc ttgttacgca gcccaccacg
    19201 ggacaaccgt ttggcgaacc ggtacctgat ggtggatacc gcctgcgcag ggatgaccct
    19261 atccggcttg accagttgac aattccacat gttatgatct aatcgccact gacatcatgc
    19321 tcgccacctg aaaccattcc ggttcaaaac gccttgatgg aagcacgacc acctgccttg
    19381 ctgcgcacgt ttctggcatt aatggtgcct ctcgtcacct ttttgctcca atggtcgttc
    19441 tggtcgttca tccagcctta tgcctggctg ctttttctgc ccgcagtgtt catcagttcc
    19501 tggataggtg gactgactcc cggactgctg gccacgatcc tctctgcaac catcgtcacc
    19561 tggttctttg ttccccccca gttcacgttg ctgggaaaat cgccgatggc actgatttcg
    19621 gtaatcattt tcatcgccat ggggacgctg ttcagtatct tccactggcg cctggcaagg
    19681 acgaaacaac aggctgcaga agccggtgcg aaactgaagg aaagcgagga aaacctgtcg
    19741 gtcacgctga actcgatcgg cgacgcggta attgccaccg atgccgaggg gcgggtcaca
    19801 cgactcaacg ccatggccga gcagctgact ggctggtcga atgccgaagc atccggcaaa
    19861 ccggtcgaca acatcttcag gatcatcaac catgcgacgc gtgaaccggc aatcatcccg
    19921 gtagcagcca cgctcgaaca cggcaccatt cacggccttg ccaacgatac cgtattgatc
    19981 gcacacagtg gcgcggagac gcccattgct gacagttgcg cccccatacg cagccgcgag
    20041 ggtcaggtga tcggagccgt gctggtcttc cgggacgtta gccaggaata cgccgcacag
    20101 caggcgctcc aggaaagcgc cacacgcatg cgtaccattc tggacaccat ggcagacgga
    20161 atcatcagca tcaacgaaaa agggttcgtc gagatggtaa atccggcagc cgagcgcatt
    20221 tttggttatg cagcggagga actgatcggg cggaacgtca gcatgctgat gcccgagcct
    20281 tatcgcagtt cacacgacag ccacctcgaa cgctacatga gcacaggtca ggcccacatc
    20341 ctcgggattg cccgcgaatt tgagggcctc cgcaaggatg gcggccgctt ccccctcgaa
    20401 ctgacggtca gcgagatgtt gctcggcagc acgcgccatt tcgtgggcct gacacgtgac
    20461 atgacggcac gcaggcaggc cgaaaacctg ttcaaccggt ttttttcgct ctcgctggac
    20521 atgctgtgca tttccagcgc cgatggctat ttcaaacgcg tgaatccggc attcacggaa
    20581 acactgggct ggagtaccga cgaattgctc tcccgcccct ttctggacct ggtgcatccc
    20641 gatgatcacg cagccacctt gcgggaagtt gccgaacaaa ccgaagccgg caagatcacg
    20701 ctgaaattcg aaaatcgcta cagacacaag aatggctcct ggcgcatgct gtcctggaca
    20761 tcaatcgccg atgacaccgg gctcatgtat gccaccgcac gcgacatcac tgatcacagg
    20821 cagatgacgc aaagcctgat cgcggcaaag gaacaggccg aactggcgaa ccatgccaag
    20881 gactcgtttc ttgccaccat gagtcatgaa atccgtacgc cgctttccgg catgctgggc
    20941 atgctggaac tgttatcgct gacttcactc gatgaagaac agcggaacac cctggatgcc
    21001 gcctgggatt ccgcaagggg gctgttgcgc attgtcagcg atattctgga ctggtcaaag
    21061 atcgaggaag gaaaactcga actcgccccg cgcccgacgt ccatcgagca gctgctcagg
    21121 gatgtcatca acgcatattc acgagtggcc agcgccaaat ccctgatgct ctggcaacac
    21181 acggatcccc ggctcggttc cgcatatatt gccgacccgc tgcgactctc ccaggtactc
    21241 aacaactttg tcagcaacgc gatccggttt accacagtcg gcgagatcga ggtgcgggcg
    21301 gaactgctcg aacacctcga cagtggcgac cgtatccgtt tctcggtcag ggacaccggt
    21361 accggcattg acaaggccgt acaagagcag ttgttccagc gatatcgtca gggaagtgcg
    21421 gacacagcac gcatgtatgg cggtaccggg ctggggctat cgatctgcaa gcggctgact
    21481 gacctgatgg acggacagat cagcctggac agcacgcccg gtctcggctc ggtcttcagc
    21541 atcgtcctga ccctgcccat ctcggggctg cccggggaag cgatcaaaaa catgtatcct
    21601 gaagccaggc agcgggacat ccagccgctc ttcacgaaca gcctcgactc ccccctcatt
    21661 ctggcagtcg atgatcaccc gatcaatcgc gacctgctga cacgacagct ccagttactt
    21721 ggcctgcgca gcgagaccgc ggaaaatggc aggctcgccc ttgccatgtg gcagaaaggc
    21781 aactacgccc tgatcatcac ggactgccac atgccggaga tggacggata tgcattcgtc
    21841 agggagctgc gcagaattga agccagcgca tcccttccac acatacctgt cgtggcctgg
    21901 acagccaacg cacttgccga ggaagcacaa cgctgccagg atgccggcat ggatgacctg
    21961 ctggtcaagc ccgccgacat gacccaactt gggcatgtac ttgaaaaatg gctcgacctt
    22021 cccgatgagg ccggaaaaga tggtctggca acgatgtttg accgtaacgt gctgaatacg
    22081 atcataacgg atgaatcagc acagtccggg ctgttgaatg acttcatcag acacattcag
    22141 gcggatcaca gcaagctggc cgatgcttgt ggcgcgggca gtctcactga catcgagcat
    22201 atcgctcaca agatgaaagg ggcgagccgc atggtcggcg cacgcgatct ggcagaggca
    22261 tgcgaagccg ttgaaggtgc cgccaaagcg ggcagcatga cggatgccat ctccgcaagc
    22321 gcagtactga gggaggcgat ttcacgggtg acagccaccg tcgctgccgc aacgaaatga
    22381 ccccattccg tatgcaccaa acaaaaacgg gacggcattt gccgtcccgt ttttgtgtct
    22441 ttctgactat ccggttgatt acgcaatcta tttacattgc atttacccgg attgccagta
    22501 taatgactgg tgcccagaag aggactcgaa cctccacacc ttgcggcaca tggacctgaa
    22561 ccatgcgcgt ctaccaattc cgccatctgg gctccgatga agtgcagcat tataaatgaa
    22621 caagagcagt tgtcaatgac ccattccgga tttccggaaa aaagaaccgg cgctgacaac
    22681 gctcacgcca accaggcaac ggccgtcgcc tctttccgct tacccggacc cggcgcacgc
    22741 ccccacacga agaacaagga acagcatcaa tgacccaatc cggcaattcc aggaaaaaac
    22801 gcaaggaaca gaacctgcgc caggaagacc cccacctcga acgcgaacgc gaacgctatg
    22861 aacatccctt gccgagccgg gagttcatca tggaaatcct gcgccgccac ggcactccac
    22921 tgtcagccgt cgatctggcc gggctgctcg acatcaccga agaggaagcc gacctgttct
    22981 cccgacgcct gcgcgccatg gagcgcgacg gcgaactgat ccagaaccgc aagggtgacc
    23041 tgtgcgtcac ggagaaactc gacctcatca agggcatcgt gcaaggccac cccgacggtt
    23101 tcggttttct cgaacccgaa gacggcagtg aatccctgtt tctctcaccg cgggaaatgc
    23161 agaaagccat gcacggcgac atcgtgatgg gccgcgaaat cggtctcgac cgacgtggcc
    23221 ggcgcgaggg caagatcgtc gaggtgctgt cgcgcaccac cacccacgtg gtcggccgtc
    23281 tgctcatcga gcacggcatc atgctggtgg cgccggaaaa caagcgtttc acccaggaca
    23341 tcctcattcc gtccggtaac caggcacatg cccgtgccgg cgaagtggtc aacgtcgaac
    23401 tgatcggcca ggtcaaccgc aacgtcaaac cggtcgggcg cgtggtggaa gtgctgggtc
    23461 agtacaccga cccgggcatg gaaatcgaga ttgcgctacg caagcacgcc ctgccctggc
    23521 tgttcccgcc ggaggcacgc gaggaagcgg aaagcctgcc cgcccgggtg cgcaaatccg
    23581 acatggcaga ccgcgaggac ctgcgccgca tgccgctgat gaccatcgat ggcgaaaccg
    23641 cgcgcgactt tgacgacgcc gtgtattgcg aaccacatgc tggcggcttc cgcctggtgg
    23701 ttgccattgc cgatgtctcg cactacgtgc ggcccagttc tgcgctggac aaagaatcat
    23761 tcaatcgcgg caattcggtt tattttccgc gccgcgtcat ccccatgctg cccgaagagc
    23821 tgtcgaacgg cctgtgctcg ctcaacccgc aagtcgaacg gctgtgcatg gtgtgcgaca
    23881 tgcagatcgg caaaatggga gccatccaga gttaccgttt ctatccggcg gtgatgttct
    23941 cgcatgcccg cctgacctac aatcaggtat gggactggct ggagcatccg gaaaatgccg
    24001 acgagcatgc ggcactgctg ccacacctgc agcagctgca tgaactgtac aaggtgctgc
    24061 tcaaggcgcg cgaacgtcgc ggcgcaatcg acttcgaaac cgtcgagacg cagatggtct
    24121 tcaacgaaga gagcaagatc gagcgcatcc agcccgtcac gcgcaatgac gcgcaccgca
    24181 tcatcgagga atgcatgctg gcggcaaacg tatgcgcttc ggccttcctg aaaaagcatc
    24241 gccatcccac gctgtaccgc gtccatgaag gcccgaccac cgaaaagctc gccgcgctgc
    24301 gtgaattcct cgccgagttc ggtttgggcc tgtctggcgg cgactcgcca caggcgaagg
    24361 attacgcacg actgttgcaa cagatcaagt tgcgtcccga cgcacacctg ctgcaaaccg
    24421 taatgctgcg ttcattgcag caagccgtat acagccccga gaatgccggt cactttggac
    24481 tggcctatga acactacacg catttcacct cccccatccg ccgttatcct gacctgctgg
    24541 tgcacagatc catcaaggca gcgctggaca acaaaaaata tatgccgggc agctggcaag
    24601 ccatcggcgc gcactgctcg atgaccgaac ggcgtgcgga tgaagccaca cgcgatgtcg
    24661 aatcctggct caaatgcttc tacatgcagg accgggtcgg cgaagtgttt tctggaacca
    24721 tcgcctcggt caccggcttc ggcctgttcg tcacccttga cgaattgttt atcgaaggac
    24781 tggtgcatat ttccgaactg ggcagcgact actttcattt cgacaagacc cggcatcaga
    24841 tgctcggcga acacaccggc gcgcgcttcc gcctcggcga tcgcgtcgaa gtgcgcgtaa
    24901 tgcgcgtcaa cctcgacacc tcacaaatcg atttcaatct gacgcagacc ggggaaaccc
    24961 cgcgcaaaca gccgcgcaac gcgaaggaag acacgtctgc gcaacccgct gcaccgagga
    25021 aacgcgcatc gaaatccgac gcgacacgtg ctgcgggtga cgggagcaaa ggccgtcacc
    25081 gcaaacgggg gcgctgacat ggcagacatt ctgatctacg gctttcatgc cgtcacggca
    25141 cgcctgcgta gccagcccac aagcgtggaa gaaatttacg tcgatgccca gcgtcaagat
    25201 gcgcgcatgc gcgacctgca ggagcaggcg cgccagttgg gtatccgcat cgtgccactg
    25261 gagagtcgcc ggctggacgg tatggccggc ggacctcgcc accagggcgt cgccgcccgt
    25321 gtaagcatgg cccaggtgcc acgccacatc gatgacgttc tgcagcatgc caccggcccg
    25381 atcctgttgc ttgtgctgga cggcatccag gatccgcaca accttggtgc ctgtctgcgc
    25441 atcgccgatg ccatgggcgc caacgccgtc attgcaccgc gtgaccgcgc tgtgggcctc
    25501 acccccacag tggccaaggt ggcgagtggc gcagccgata ccgtgcctta cctgacggtg
    25561 gtcaacctcg cgcgcgcact gcgcgagatc aaggagtaca atgtcactgt cattggtacc
    25621 gacggcgatg cagaaaccac tctgcccgca acagatgcca gcggctcggt ggcctgggtc
    25681 atgggagctg aaggcagcgg catgcgccgt ctcacacgcg aacactgcga cgtgctggtg
    25741 cgcatcccga tgtatggcac cgtggccagc atgaacgtcg cggtcagcgc cggtatctgc
    25801 ctgtatgaaa cccgcagaca acggagcttg catgaacact gaccacgcct ggcgcatttt
    25861 cgattccgcc gaacttgtct gttctgccga aaccgttgcc aaggccatcg acaaactggc
    25921 cgccgatctg cgcgccgatt atcgcaa
//
LOCUS       DAIANL010000124        21302 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1991046, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000124 DAIANL010000000
VERSION     DAIANL010000124.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 21302)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21302)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21302
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991046"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3756 a         5849 c         7281 g         4416 t
ORIGIN      
        1 gcgacatagg cgcgggcaat gtcgcgtaca tcggaaaaat cgcgccacac ttccaggttt
       61 cccagttcga tgtgcggcgc gcgcgtggca tagtggtgca cgattttcgc aatcaggaag
      121 ctgatgtcct gtccggcgcc agtgtagttg aagggccggg tgaacacgat gggcagcctg
      181 tcgagccaca ggcgcgccat gtattccatc gacagtttgc ttacggcgta gtcgttggcc
      241 ggccgcagcg gagcgttttc gtcaagtatg cccgattcga ggtggccata gatgttggcg
      301 ctgctggcga ggatgacctt gcgtggcgtg ttgccggcgg tggccagggc ttcgagcagg
      361 tgccgggtgc cgatcgggtt ggtgtgatag atctgctcgg catcgtcatg tgcaacgtgg
      421 gcgatggcag cgagatggat gaccacatcc ggtgcaatgt cggccacggc acgcagcacc
      481 tgctggcgat gttcaagcgc gatgcgttgc ccgttttcat cgcgcccgag accggtgaca
      541 cgatagccag cggcttccag cccggctgcg acatagcgtc cggtaaagcc ggccaggccg
      601 gttacgagcg caatgttgcc ggctggtggt tcagctgcgg acgccatggc tggctctggc
      661 aagatcggct tccaccatca tggcacacaa cgattcgagg gaggtctgtg cctgccagcc
      721 cagttcggcc cgtgcttttg ccgcatcgcc gatcagcagt tcgacttcgg tgggacggta
      781 gaaggctgga tcaacgcgca ggatggtctg accggtcgcg ggatggatgc cgatttcgtc
      841 ggcgccctgg ccttcaaagc ggatgtcgat gtcgacggcg cgaaacgcca tgcgcacgaa
      901 atcacgcacg gtttcggttc ggccggtggc caatacgtaa gtgtcggggt gggctgcctg
      961 cagcatgcgc cacatgcctt cgacgtattc cttggcgaaa ccccagtcgc gtttggcatc
     1021 gagattgcca aggcgcagct cgtccaactg gccgagatga atgcgcgccg cggcatcggt
     1081 gatcttgcgg gtgacaaatt ccagcccgcg cagcggtgac tcgtgattga acaggatgcc
     1141 gctggaggcg aagatgtcgt aggattcgcg gtaattgatc gtcatccagt gcgcgtacag
     1201 cttggctgcg gcatacggac tacgcgggta gaatggagtg ttttcatgct gcggaatggt
     1261 ctgcaccttg ccgaacattt cggaggtgga cgcctgatag aaacggatgc ccgggtcaac
     1321 gatgcggatt gcttccagca ggttggtcgc gcccaggccg gtgatctcgc ccgtggtgac
     1381 cggttgggcg aacgatacac caacgaagct ctgcgcggcc aggttataga cttcatcggg
     1441 ttgtgcatgc tgcagcaggc ggatgttgtt gcccaggtcg gtgagatcgt attcgaccag
     1501 atgcaggttg ggatgccggt cgacacccag ttccgccaga cgccagaaac tcaccgagct
     1561 ggtgcggcgg taggtgccat ataccgtata gcctttgccg agcagcagct cggtcaggta
     1621 ggcgccatcc tgtccggtga cgccggtaac gattgcagtg gtcatgatgt cagattccac
     1681 atgtgttgat tgaatgcggt tcagttgcgc aagtagatgt cttcatagcg cacgatgtcg
     1741 tcttcgccca gatagctgcc ggactgtact tcgatcaggt gcagcggtac cttgcccggg
     1801 ttgatcagcc ggtgtctgct gccgagcggg atatacgtgg actggttctc cgaaaccagc
     1861 ttttcttcgt cgccatggac caccagagcg gttccggata ccacgaccca gtgctcggcg
     1921 cggtgatggt gcatttgcag cgacagcttg gcgccggggt tgaccatgat gcgcttgacc
     1981 tggaagcgct cgcccttgtc gatgccctcg taccagcccc acggacggta gacgcggtca
     2041 tggtacaggt gctcggcgcg ctgctgtgcc ttgagccggt ccacgatacg cttgacatcc
     2101 tggctgtggg atttgtgcgc caccaatact gcgtcggcgg tttcgaccac caccagatca
     2161 ctgacgccaa ttgcgaccac cagccgcgat tcggcgcgaa tcaggttgcc ggaggcggat
     2221 tcggtcatca cgtctccgcg gatgacgttc aattgttcgt ccttgacgct gacgttccac
     2281 agggcatccc aggcgccaat gtcattccag tcgaagctgg atggcaccac agccgcgcga
     2341 tcggtatgtt ccatgactgc gtagtcgatg gattccgacg gacaggcggt gaatgcttcg
     2401 ctgcccagcc gggtgaaatc gagatcgtgc tgtgcggcgt ccagcgccgt gcggcaggcg
     2461 tcgagaatgt cgggacgcag ttggtgcaat tcttcgagaa tgcggtcggc gcggaacagg
     2521 aacatgccgc tgttccacga gtactcgccg ctgtcgacgt agcgctgtgc ggtagcgcga
     2581 tcgggtttct cgacaaacga cagcaccttg cgcgcatggg cacagcctgc gagggcctcg
     2641 ccctggtgaa tgtagccgaa tccggtttca gcagagcggg cctgaacgcc aaatgtggtg
     2701 aggtaaccgt gtgcggcggc ctcgatggcg tgagtgatcg aggtggcaaa tgcctgcgca
     2761 tcgtggatga cgtgatcggc gggtaacacc agcatcagcc cctgcgggtc gcggcgggca
     2821 acatcgagcg cggctacggc aatcgcagcc gcggtattgc gaccgaccgg ttcgagcagg
     2881 atcgatgctg gctgaacgtc gatggcgcgc aactgttcgg cgataaggaa acggtgttcc
     2941 tgattgcaga cgatgaccgg tggcgcggtc tggatcacgc catccagccg cagtacggta
     3001 tcctgcagca gcgtccgttc ggtcagcagc gccagcaact gcttgggcaa tgccgcgcgc
     3061 gacaacggcc acagccgggt acccgagccg cctgacagca cgacggggtg gaggattgca
     3121 ttcatcgaaa attcctgtgg tgccatagcg ggtagatcat agcatggggc tgtggcaaat
     3181 tcctgcacga accgacaggg tggccgatga catggtcatg cgtccgtgtc accggacgat
     3241 tccccggtct cgtcggccgg ctcctccgtc gcggtcggcg cggcaatctg gcgcaacagt
     3301 ctgaacagcg cccggttgct gcgcggcggc agctgctgcg cggcttcctt gcgcgcatta
     3361 cggatcaggg tgcggagtgg ctggaggtcg gtcagcgggt agcgttcgat gaattgtggc
     3421 aggctggcat cgtcggcaat gagccggtcg cgccagcgtt cgagctggtg gaagtgggca
     3481 ttgtcttcgt tgtgccgcgc ctgccagcgt tgtaactgtt ctgcaaccgg ctgggcgtca
     3541 aattcgcgca tcagccggcc gatatattgc atctgccgcc gtaacccgcc gtgggcgtgg
     3601 atgcggcgcg cttcgtcgat ggcggcgcgc agtgtgtctg gcagcgacag gcgatcaagc
     3661 ttgtcctttg gcaggctgac cagtgtttct ccgagttttt gcaattcggt catctggcgc
     3721 ttgagcgcgc tcttgctggg cggtgcggat tcgtcaaagg gttccatggc gcacggtatg
     3781 gggaaaatgt tgttatgata acggttttgg ctctgggcag ggtgacgcag tggcggcttc
     3841 ggaacagttt ggtcatgaca gggaagcgtt acagacgctg gtgcacagga tgctggatca
     3901 cgcagcgcgg caaggggcga gtgcgtgtgc tgccgaggct tcggtgggca tgggacaggc
     3961 ggtgacggtg cgcaaaaacg aagtcgaaac catcgaatac aaccgcgaca aggggatttc
     4021 ggtgacggtg tacctcggcc agcggcgtgg ccatgcctcg agttccgatt tcggggagca
     4081 ggcgctgcgc gatacggtag acaaggcgct gaccattgcg cgctataccg ctgaagatcc
     4141 gtgcgcggga ttgccagagg ctgacagcct ggcggtgacc aggccggacc tcgacctgtt
     4201 ccacccgtgg acgctggatg tcgacgaggc gatcgcgatg gcagccgagt gcgaggctgc
     4261 cgcggtaggt gtcgatccgc gcatcagcaa ctcggagggg gcaacggtca gtacgcagca
     4321 gtcgctgttc gtttatggca acagcctggg gttcctggcg ggctaccctg catcgcgcca
     4381 tggtgtgagc tgtgcggtga tcgccggcga tgaccagggc atgcagcgtg attactggta
     4441 cagttcggcg cgttgcgccg aggatctacc gccggcggca gaggtcggcc gacgtgctgg
     4501 cgagcgcacc gtgcgcaggc tgggtgcccg tcgcctgagc acgcgcgagg tgccggtgct
     4561 gttcgaagcg ccggtggcga cggggctgct gtcgcatttc gtctctgcaa tcagcggcgg
     4621 cagcctgtac cgcaaatcgt cgtttctgct cgacagcatg ggacgtcagg tgtttcccga
     4681 gtgggtgcag atcgtcgaac ggccacacct gccgcgcgga ctcgccagca gtccattcga
     4741 taacgaaggt gtgcgcacgg tcgatcgcga cgtggtgcgc gacggtgccg tgcaggggta
     4801 ttttctttcc agttacagtg cacgcaagct ggggctggct acgaccggga acgcgggtgg
     4861 cagtcacaat ctgctggtca acagcagtgg tgaagacttc gccggattgc tgcggcgcat
     4921 gggcagcggt ctgctggtga ccgaactgct cggtcatggc atcaatctgg tcactggcga
     4981 ttattcgcgt ggtgccgccg gttactgggt ggagaatggc gaactgcagt acccggtgga
     5041 agagatcacc atcgccggca atctcgcgca gatgtaccgc gacatcgtgg cgatcggtac
     5101 cgatgtggaa atgcgcagtt cgcggcgcac cggttcgatc ctgatcgggc gcatgaccgt
     5161 tgcaggggac tgagatgcgt ttctggctgc ggatcgcgcg gggtatcgac gcgctgaccg
     5221 aaggtgtcgg tcagacggtc aagtggctgg tgctggttgc ggcgttgata tcggccggaa
     5281 acgcgctgtt gcgctacggc ttcggtgtgg gcagcaatgg tgggctggaa ctgcagtggt
     5341 atctgttcgg cgccattttt ctgctgggtg cgggctacac gctccggcag ggtgggcatg
     5401 tgcgcatcga cctgttgcat ggccaattgt ctgcgagggc gcgctcggtg gtcgagattg
     5461 ttggtacgct gctgtttctc ctgccgtttt gcgcattgat gattgatctt tcctggccgg
     5521 ttttcgttga ctcgtggcac agcggcgaga tgtcgcccga tgccggtggc ctgccgcgct
     5581 ggccggtacg gctgctgatt cccgcaggat ttttcctgct cggcctgcag ggcgtggccg
     5641 aactgatcca tcatgtcgcc cggcttgccg ggcatgaggc catggaggac agcgaggcat
     5701 gatccagttg gcgccagcca tgtttgccgg cctggtggca attctgctca tcggttatcc
     5761 ggccgctttt tcgctggccg ccaacggcct gttgttcgcg ttgatcggcg tgaaactcgg
     5821 gttgttcgac tggagcctga tgcgcgcact gccagaccgg gtgctgggca tcgtttccaa
     5881 ccagacgctg ttggccattc cgttttttac ctttatgggg ctggtgctgg aacgcagtgg
     5941 catggcggag gatctgctga ccagcatcgg gcagctgttc ggcagcgtcc gtggcggact
     6001 ggcctatgca gtgatcctgg tcggtgtgct gctggcggcg actaccgggg tggtggctgc
     6061 ctcggtaatc gccatgggac tcatttcgtt gccgatcatg ttgcgctatg gctatgacgc
     6121 ccggctcgcg gccggtgtga ttgccgcatc gggtacattg gcgcagatca tcccgccggg
     6181 gctggtgctg atcgtgctgg ctgaccaact gggcgtgccg gtcggggaca tgtttcgtgc
     6241 ggcaatcccg ccgagcctga tgctgacggg tttctatctg ttattcgtgt tcattatcac
     6301 gctgatccgg ccacaggccg tgccggcatt gccatcggag gtgcgcacgt tgcggggcat
     6361 ggcgctgctg cgctcggccc tgctggcgat cctgccgccg ctgctgctga tcggcctggt
     6421 gctgggcagc atctttctcg gctgggccac gccgtccgaa ggcggtgcga tgggggcgag
     6481 tggtgcgctg ctgctggcgg gcatcaaacg caagttgtcg cgacggttgc tgggcgaggc
     6541 cacggaacgt actttgcgtc tgctgagctt tgtgatgttc atcctgatcg gttccacggt
     6601 gttcagcctg gtgtttcgtg gcctcaatgg tgacctgtgg gtcgagcatt tgctgaccgg
     6661 cttgccgggt ggcaccaccg gctttctcat cgtcgtcaac atcttcatct ttttcctggc
     6721 gttttttctc gactttttcg agattgcctt catcgccatt ccgctgctgg cgcctgccgc
     6781 gcagaaactc ggtatcgatc tggtgtggtt cggtgtgctg ttggggctca acatgcagac
     6841 ttcgttcatg catccgccgt ttggtttctc gctgttctat ctgcgcagtg tcgccccgaa
     6901 atccatgcgt tccagccaga tctattacgg cgcgatcccg ttcctgctca tccagttgct
     6961 catggttgcg ctggtgatcg cctttcccgg cattaccggc ggtgccagcc atccggggtt
     7021 gaagagcggc gtgatcgagg ttccggtcga tgaggatggc tacggcgact attacctgcc
     7081 gcgggaatgg cgctgaatga ctgggcggtt gctgtggcca gtcaggcgcc cggccacagc
     7141 gcccagacgc aggccgctgc ccaggcgatg acggtcagca gcaattgcca gtcggcaagc
     7201 agcgcatcgg tgggagattc gccgccttcg agcgattcga tgacgtacca gtaacgaaac
     7261 agtccgaaca gcaccagcgg cacagtgatg accaggtgcg catgggttga catgacgaac
     7321 aggctgtaga acacgagcgc gcccgtggcc gacatttccg cgtagcggtc gaccagtgcg
     7381 acgctgtagt ggcgcagcac gctgcgggtg tcgctggtac cacgcgccag ttcctgacga
     7441 cgcttgactg cggcaaggta cagcgccagg cacagcgtgg tgacgaacat ccaggcggac
     7501 accggcacgt caagtgcgac ggcgccggcg tagacccgca gcacgaaacc actggccaca
     7561 gtgaagatgt cgaccaccgg ctggtgcttg agcacgaagc tgtaggccag gttgagcagc
     7621 agatagccga tgaccacgcc cagtacgccc ggggcggcat accatcccac gccaaggaga
     7681 acggcataga ggaagccaag cagcagcagc gcctgtgccg gtcgcaccgt tccggcagca
     7741 agtggccggg tgagtgcttt ttgcggatgc tgccgatcct gttcgatgtc gcgcaggtcg
     7801 ttgacgatgt aggtggcgga agaggcgacg gtgaacagag ccatggcgat cagcgcccgc
     7861 cacacggctg cagcgtgcat gaactgtccg gcgaatatca gcggggcgag cacgaagcca
     7921 ttcttgatcc attgccgcgg gcgcatgagt tgcagcaggc cgcgccatac ggctggacgc
     7981 ggggaggggg ttccggtatc gtgcatgcga tttgaatcct gaggcgtgtg agggcagaac
     8041 agtcgatgag attgacgttg gtgtatgcga ttgtcgcatc aattgccatg atggccaacc
     8101 tggccgcgca acagcttgtg ctgacgctga ccggcggtgc gctgcggctg atgtggccgg
     8161 tggcggtcgg caccggggtc gggttgctgg tcaaatatgt gctcgacaag cgtttcgtgt
     8221 tctgtttccg tgcagccggt atcggtcacg atgcgcggct gttcgtgctc tacgccggcc
     8281 tggggctggt gaccacgctg gtcttctggg ggtttgagtt tggtttccag tggtggttcg
     8341 gtaccgccgc gatgcgttat gccggggcaa cgctgggact ggcgctggga tatcttgcca
     8401 agtttcatct cgaccggcgc ttcgtgttct gcgggacgca gtcatgaagg gcgtgtattc
     8461 ctggggccgg ctcgcactcg agccgcaccg ggtgatcgac ctgcacgatc cggctgagct
     8521 ggctgcgcag cttgaacaca atcgccccgg tatcgcgtat ggtcttggcc gcagttatgg
     8581 cgatgcctgt ctggcgcctg gcgatacgct gtggcgtact ccgggactgg ggcgtctgat
     8641 tgcgttcgat gcgcagactg gcgtgctgca ctgcgaggca ggcgtgaccc tgcgcgagat
     8701 tcagcgtctg ttcatgccgc gcggctggat gctgccggtg actccgggaa cccagtgggt
     8761 tacagtgggc ggggcgattg ccaatgacgt gcatggcaag aaccaccacc gtttcggttc
     8821 gttcggcgat catgtgcgtg cgctgcgcct gctgcgtacc gatggccagc agatcgagtg
     8881 cggaccgcgg caggagccgg gctggtttgc agcgacgctg ggcggcatag gcctgaccgg
     8941 gctgatcgtc tcggccagcc tgcagttgcg ccgcatcgac agtccgtggc tgacggtgca
     9001 gacgctgccg tatgccagcc tggaggagtt ttttgccctg tccgatgcat cggagatgga
     9061 ctgggagcat acagtgtcat ggatcgactg cttcagtcgt ggcggccggg gaatcttcat
     9121 gcgggccaac ccggcaccgg ctggcctcgc tgcgccggca ccggggcgcg aaaaacgcgt
     9181 gccgtggacg ccgccattgt cgctggtgaa ccgctggagc ctgccagcgt tcaatcttgc
     9241 ctattatgcc ttgcagcggc gccatgtggg ccgggaacga caggtggcgc acgaaccgtt
     9301 ttcctgtccg ctcgatcagg tgcaggaatg gaatcgtctc tatggcccgc acggtttcta
     9361 ccagtaccag agtgtggtgc cacggcgcga tgcgcaggcg gcgctggctg ccatgcttga
     9421 cgcaatcgcc cgctccggtg aaggatcgtt tctggcggtg ctgaaaacct tcggtgaacg
     9481 gcgttcgctc ggcatgctgg gtttcccgca gccgggagtg acgctggcgc tggattttcc
     9541 caatcgcggc gcgcgcacgc accggttgtt tgccagcctg gacgccattg tgcgtgaggc
     9601 aggcggcaaa ctctatctgg ccaaggatgc acgcatgccg cgcgcactgt tcgaggcggg
     9661 atatcccgaa ctgaatgggt tccagtcctg gcgcgatcat ggcatgcgct cgtcgatgtc
     9721 gcgtcgcctg atgggagact gattcgtggc agcaggttgc tgtcacgtcg agaaaggcgg
     9781 aacatgaaac atatcgtgat cgtgggggcg acatcggcca ttgccgaaca gtgtgcccgt
     9841 ctgtggctgg tgcaggagcc ggcccggctg gtattgctgg cgcgcgatgc cgggcgggtg
     9901 gcgaggctgg cgacggattt gcgtgtacgc catccgcaag ctgcgattga gacagtggcg
     9961 gcggatttcg atgatgcaca ggccattgct gcccaggtgg cggcaatcgt tgcgcacgcg
    10021 cccgtcgata tcgtgcttat cgcgcatggc tggctgcccg atcagaccgg ctgtcagcag
    10081 gatatcgcct gctgccgcca ggcactgctg attaacggtg tgtcgccgct attgtacgca
    10141 gaggcgtttg cccagcacat ggagcgggcc gggcatgggc ggctggcgct gattggctcg
    10201 gtggccggcg atcgcggacg caagtcgaac tacatttatg gtgctgccaa gggcatggtc
    10261 gcccgttacg cagaaggcct gcagcaccgg tttgccgcca gcggtgtcag ggtggtgctg
    10321 gtcaagcccg ggccgaccgc cacgccgatg acggctcaca tgcccgggca gggcagcatg
    10381 gccagtgctg ccgaggtggc cctgcgcatc gtgcgtggtg ttgaacgggg tcaggccgtg
    10441 gtgtatgcgc cgccacgctg gcgagtcatc atgtgggtga tccggcattt gccagccgtg
    10501 gtgttcaacc ggctcgatat ctgagcgtcg cccggtcagg ggctacgctt gtcgcggaag
    10561 aagtgccaca gcgtgtcggt cgcatcgagc gcatggctgg gagggtcggg cctgaacaga
    10621 cgggcggtca ggccgcccag cggcgtggcg ccgggccact ggtggcctgc accgtcaatg
    10681 gtgatcagca ccacctgcat gtcgtcggcg catgcggcgc tgtcggtgac taccgtgccg
    10741 ttgcgggtgt cggtcggcgc agcgcactgg tcaagaacgc ggaaccgttc gatgccctgg
    10801 cgtaccggtt tccagatgat gtgactcagg gctttggggc cttcgccgcc gtccatcggg
    10861 atgttctggt ccgccagtcc gtgaatttcc atgaccgata ctggtgcagg attggcgcac
    10921 agatcagtca gttctccgga gaccgatccg atggcggcaa gcggaaacgg gctgtcgcag
    10981 gcatagcgcc atgccatggc ggcgccattg gagaaaccac tgacgtagac acggtgcgga
    11041 tcgacatgct gcgtgtggat gagtgtttcg atgatggcag tgatgaaccc gctgtcatcg
    11101 atatggttgc gcagcgccgg gccacaacat gctccaccgg cattccagct gtttccgagg
    11161 ctttccgggt aagcgacgat gaatccatag gcatcggcct cacggtccca gtgtaacgtg
    11221 cgctcggcct gtcttgccga acccagacgg ccatgcagta cgactaccag tggtgcggcc
    11281 tgatcctgtg ccagaccggg cggtcggtag accaggtagc tgcggtcgcg ggaaccgatg
    11341 tggagggtat acgtttcacc ggccagtgcg gcaagcgaaa tcaggcatag catcagtgtc
    11401 agcagagggc gcgcaaggca agacatgcgg aatcctgtgg cgatgacggg tgtgcctagc
    11461 ataggggaac tggcaggatg aaacaagcgt acggttgtgg cgaaagcctc tggcgcgcgc
    11521 agagggccct gggtcaggca gcctgcagcg ggatatgacg gcgctggtcg atgatgcggt
    11581 tgtctgcatc gacgtagacc agtcgcggcg caaactcgtt gagttcgtgt tcgtcaagcc
    11641 gggcataggc ggcaatgatg agcaggtcgc cggggttggc tttgtgtgcg gctgcgccgt
    11701 tgaccgaaat gatgccggaa tcgcgtgctg cgcggatcgc ataggtggtg aagcgttcac
    11761 cgttgctcac gttgtagatg tcgatttgct ggtattcacg catgcccgcc gcgtcaagca
    11821 gggtctcgtc gatggcacac gaaccttcgt aatgcagctc ggagcgggtg acgcgcacgc
    11881 ggtgaagttt tgaaaacagc atggtgatct gcatgatggc aaattccggt gatgtttgcc
    11941 gttatcgtgt cgatgcaggg catgcaacag cgattcccgg cgcgtgcgac aggcgaatgc
    12001 tacgtgcctg cccatgaggg tgttggcgga gtggacgggg ctcgaacccg cgacccccgg
    12061 cgtgacaggc cggtattctg accaactgaa ctaccactcc tgcatgcatt ccgttcggcg
    12121 catggcgcga ggctggtgcc cgcgcagtaa atctggcgtc cccaacgaga ttcgaacgcg
    12181 tgtgatcgcc gtgaaagggc ggtgtcctag gcctctagac gatggggaca atgcaatttg
    12241 actgccgtca gaactgcaac ggttgcaata cggctgcaag gtcagtcaaa tttcataagc
    12301 tgcgcattgt agcagcgatt tatcgttttg agaatagggt gatgacaaca ccggtgagca
    12361 gaccgagaaa actggccggt accatggcca gccagatgct gttttctccg tagacgacga
    12421 gatcgcggaa acccatcacc agaccgacca cgcaggcgat gatgctgatc cagttcacgg
    12481 cgacgccgat tttgtgcatg tgcaggctca cattaaaagt ttagtcgggc agcgatttgc
    12541 atgctgcggg ggcgcttccg gtgacctcgc cagttgcggg cgggatggcc ggaaaccatt
    12601 ttcctctgtt ccggccggtt gtatccactt ttttgtggca gggcaagatc ctccgggtac
    12661 agaatgtcag gagaatgctg cgctggaccg gcttgacgcg gctggtggct ggacagcgcc
    12721 agtgaacgca agtattgtac ttgtctgtca tgtgttttgt gaagcattgg tgtgcccgac
    12781 aaaatacagt gcgaccaaga ccaacagact ggaatcggga gaatccggca tgagcttcgt
    12841 gagtgaattc aaggaatttg cagtcaaggg caatgtgatc gacctggccg tgggtgtggt
    12901 catcggcggc ggtttcggca agatcgtcga ctcgctggtc aaggatgtgg tgatgccggt
    12961 ggtgggctgg ctggttggcg gggtcgattt caaggacctt tatttcagtc ttgaccggca
    13021 atcgtacgcc acgctggccg cagcggagaa atcaggtgcg cccgttgtgc gttatgggtt
    13081 gttcgtcaac agcgtggtcg attttttcat catcgcgctg gtcatatttt cggtcatcaa
    13141 actgatcaac cgcctcaagc gtccgccggc acctgcccct gtggccgaac cgccgacgcc
    13201 ggaggatatc ctgttgctgc gagacatccg cgacagcctg cgcaggcagg gtggacatgg
    13261 aaccggaagc tgaccgccgc cctggcgttg cgccggttgt ggaccatggt tatcaacgcg
    13321 gtatccgtgt caatctgcac cagtttctgc aacatggact gcaactgttt tttgtcggtc
    13381 tgatgatcgg tatggagcgc aacgtgttgc cgctcattag ccgggaattc ggcgtggcaa
    13441 aaggctcgtt tctgtacctc atggcgttcg tcgtgtcgtt tggactggtc aaggcggtgc
    13501 tgaattttgt tgccagccat ctttccgagc gtatcggccg caaacgggta ttgctgatcg
    13561 gctggctggc tgcattgccg atcccgtttc tgatcctgta tggacacagc tggatgtggg
    13621 tggtggtggc gaatgtgttt cttggcctca atcaggggtt ggcctggacc atggcggtga
    13681 ccagcaatgt tgacctgtcg cgtgccagtc agcgcgggtt tgccactggt ttcaacgagt
    13741 ttgccggtta tgccgcagtc ggactgggcg gcatggtgat cggctatggt gctgcgctgt
    13801 ttggcccgcg cctcgccctg ttctggtttg ccttgtcggt gacgctgctg gcgggcatgc
    13861 tgggcaagat tttcctgcgc gacacattgc cgtgggcgcg ggccgagcac caggaatttg
    13921 ccagtggccg ggcggtcgag cacatgccgc gctttcctgt cgagctgggt gcgaatgcgc
    13981 gcgcacgcga tgtatttctg ctggtctcgt ttcgtcatcc gacgttcagc gcattttgtc
    14041 aggccggaac ggtcaacaag atcgccgatt cgctgctgtg ggtgctgttc ccgctgtatc
    14101 tgcatgagcg cggcctcgat gcggtgcata ttggctgggt ggtggcggta tacgggctga
    14161 tatggggcgt attccagctg tttaccgggg ccatgtctga ctggatcgga cggcgcaaca
    14221 tgatcatcgc cgggcagtgg gtgctggccc ttggcgtgat gatggtgctg caatttcagg
    14281 gtctggcctg gtggctggct gcggctgccg tcatgggact gggcatggcg ctgggttacg
    14341 ccaatctcat tgcggcggtg gcggacattt cacggccggg ctggcgcagc ctggcgctgg
    14401 gcgcctaccg gttctggcgc gacatcggtt atgtgtttgg tggtgtgctg ctcggggcgg
    14461 tggcgcagtc cggtgcccgg ctggaaccgg cattctggtt gactgcgagt ctgctcgtgg
    14521 tgtccggagg cgtagtgttg tggcgtgccg aggaaacgca tccgcgtctg aatccggcat
    14581 tgccgtgaat tgtggctgga gtgaatgatg tcggggatga tggagttgta tgatgatggg
    14641 cgcgagccgt cgcgttcggc gcggctgttg gcacaggtgc aggccaatcc ggcgcttccc
    14701 gcgctgggca gcgccgtggt gcaggtggta cagattgctt cgggggatga tgatgcggtg
    14761 cagaaactgt cgcattttat cctctcggat gtttcgctga cacaaaaggt gctcagcgct
    14821 gccaacacag tggagtaccg cacccagtcc cgaccgcagg tcactacggt atcgcgggcg
    14881 attttcctgc tggggttcga tgcggtaaag accattgcgc tggccatgtt gctggtggaa
    14941 gggttgaccg gagcggcggc gcagcttgcg cgccaggaat tgcagcaggc gctggctgcc
    15001 agcctgacag ggcgcgaact ggcgaaacgc agtggacgcc gcgatggcga ggaagccgcc
    15061 attgtcgccc tgttcaagaa ttttggtcga ttgctggtgg cggtccacga tcctcgcggt
    15121 tatcaggaga tcatggcgca atcggccgaa gccgacccgt ctgcggtcgc gatccgtgtc
    15181 ctcggttgcg gcttcgacac gctggcagaa gccgtgctgc gggactggca gatcccgggc
    15241 acgatcatcc aggcgctggc gccgctgccg tcgggcgtat tgcgccggcc cagcggtgaa
    15301 caggaatggc tgcagcacgt gggcggcttc agcgcggaac tggccagccg cctgttcagc
    15361 agcacaccgg gagacctggc tgttcggcag cgccagttgc tcggtcgcta tggcgcggcg
    15421 ctgcaactcg atgcgcgggc gctggacgaa ctggcccgct acgtggcgca ggaagcgact
    15481 gcgctggcgt acagcattga ccctgccggg ttggccgcgc cgaaggggga tggcggggca
    15541 acaaccccgg agacgacgga tcttcatgaa ttcctgttga cgccgcacac cggcgatgca
    15601 gccgagatac acccggaacc ggagtgctat ccgagcggca agccgctgca tgcgaatgac
    15661 ctgctgctgg ccggagtgca ggacatcacc gaggcgcttg ccgcaggcaa ggtgtcgcgc
    15721 gaggcattga tgatgctgtt cgccgaagtg ctgctgggtg cgatggggtt tcgtttcgtc
    15781 gccgtgtgcg tgaaacagcc gggggtgcag cagtttcatg cgcaggttgc cgtcggtgag
    15841 aacggcatgg cgcggcgcaa tggtttcgtg ctgccggtgg ccggcggaaa cgacctgttt
    15901 catctggcca tgcagaacaa cgtcgatatt accatcagcg atgccagtca gcccaatgtg
    15961 cgagcactgc tgccaggggc gtgcgctgcg ctgaccgctg gcgcgttgag cctggtgatc
    16021 ctgccgttga tactgggcgg acggccattc ggtttcattt acgccgaccg ggcacgggtg
    16081 gcacccgaag gcgtgtcggc cggggaggct gcgctgatga aaacactcaa ggcacaattg
    16141 ctggcgcggc tggcatgagt cgtgtgccag gcacactgaa ctttttcggg caggaagcgg
    16201 taatatggac agggaaatcg acagcgggca atccatgaac attctgctgg ttggtgaacc
    16261 ggcagtggaa ctgtcgcagt tggcggaagg gttgtcgcaa ttgggacatc gtgtgacgca
    16321 tcagtacggt gcggccgatc cctgcgcgat cgagccaatg ccgcaggtgg tgctgcttgc
    16381 gccagagtcc gtgagtctga agacggtgtc ctgcctggcg gggtgttcgc agatgctgga
    16441 cgtgccgctg ttgctgcttg gtcagcacgg cgacgagacg ttgttatccg gattgcgtac
    16501 ctgggatgtg gctcccctgt cgtccgatcc ggccgtgctc gattgccata tccgcatgct
    16561 ggtcaggatt ggcgcactga aacggcgtgt gcgcgaactg gagaaacagc tggacagtca
    16621 gtgtgcgccc gtcgaaagca tggcggaggt cgtggcgggc gggatgtccg gccgctggtg
    16681 cagttacgaa ctgctggtcg aactgaccgt gcggcgtgaa caggcgcgtg aggaggagcg
    16741 caggcgcatt gcccgtgatc tgcacgatga aatgggacag gtgctgacgg cgttgcgcat
    16801 ggacatgtcg ctgctgcgct tgcgtcacgg gcatcaggac gagagcctgc tgcaacagat
    16861 ccagcaggcc atgaaacggg tggattcgat catccaggtg gtgcgcaatg tagcaagaaa
    16921 attgcgtcct gccgtgcttg acatgggtat tgccgcggcc ttgcaatggc aggcggaaca
    16981 tttttcgcgc cagagcggca tcgtgagcga agtggagatt gccacgggtg atttcgcgct
    17041 ggacaatgaa cgcgccacgg cgctgtaccg catcgtgcag gaatcactga ccaacgtggc
    17101 gcgccattcg cgggcggatc gggtgtgcat cagtcttggc ctgtcggatg acggttatga
    17161 actcaatgtg agcgacaacg gcattggatt cgatcccacg gatcgcagtc gcaagacttt
    17221 cggcctgatg gggattcgtg aacgggtgct gttgctggaa ggtgaactgt cgatccgcag
    17281 ccagctcaac cagggcaccc atctgcgcct gcgtattccg gccacccgtt cggggagcac
    17341 ggcatgatcc gcatccagat cgcggacgac catgccatcg tgcgcgaagg gctcaaacag
    17401 ttgtttgcca tgtaccgcga cgtgaccgtg gttggcgagt cggtgcatgg cgggcaatgg
    17461 ctcgaggtgc tgcgtcaggg agagatcgac ctggtgctgc tcgacatgac catgccgggg
    17521 atcagcggca tcgacctgat cgaacgtatc cgtgcgcatt acccgcaact gccgctgttg
    17581 gtgctgagca tgcacaatga ggcgcagatc gcgcggcggg cgatcgaggc gggcgcatcg
    17641 gggtacctga ccaaggacaa tgatccggaa atgctcatgc tggcgattcg caaggtggcg
    17701 ggcggcggcc ggtttgtcga tccgttgctt gcgcaggaaa tggtgtttga tcgggctgcc
    17761 gggacacagc gcgcgccgca cgaagcgttg tccgaccgcg aacagcatat cctgcggctg
    17821 ctggtgcagg gattgagcat caatgaaatt gccgaccagc tgtcgatcag caacaagaca
    17881 gtcagtacgc acaaggcgcg gctgatgcaa aagatgcagt tgcgcaacaa tgccgaactg
    17941 atgcggtacg gtatcgaata ccacctcgtc tgatattgac tgcccgctca aaaccgcgtc
    18001 atgcggcaaa tccggacgcg aggcatcatg accggctggg gtgaaatcag ggtacggtgc
    18061 gccccggagc ctgacgggaa ccggcacggg agtgttgcca gtcgatcggt ctgactgcgg
    18121 tggccgcgtc ggacaggata tgccagtgcc cctgtcagga aattcctaca ctgcattccg
    18181 tttttctgag ccggttttca agattgcctg atagtgtgct gcacgacttt gtttcatgct
    18241 gcgtctgttt caatgcgtca tggacatggg atgaaaggcg gaaaatccac ggagcgtgta
    18301 cgcgcgctgg tcgataatca cggcgttgcc atccgcttgc ccgcgatcga atggcattcg
    18361 ccaggcgcaa tcctggcacg gcttgccgcc gggcgtgaac gctatctgct gcgtcagctc
    18421 gaaggccgcc aggccgggct ggcggccagc gtacccggtt tcatctatac cgtgcgtcgc
    18481 gaaagcgacg gcaggacgca ttttctgttt accagccatg gcgtggaaca cctgttcggc
    18541 ctgagcccgg aggacgtgcg cgatgatgtg aacctgttgc ggcggcagta ccacccggat
    18601 gacctgccgc ggtacctgga acgcctggaa tattcgcaga ataatctgct gcctttccgt
    18661 atcgagttgc gtatttttca ccgcgaccgg agcatgcgct gggtcgagat ccgctccatg
    18721 ccgcagcgtg cggaaggggg ggcgttcgac tggcatggcc tgatgatcga catcaccgag
    18781 cgcaagtcgg tcgagcacga actgcaacgc aaggaacagg aataccggac ccttgcagac
    18841 aatatccctg atgtgatcgc gcgctacgat acacggttgc gtctgatgta tgtgaatcga
    18901 gccttcgaaa gcctgtgcgg catgccatcg tcgattctgc tgggacagca tgccgggcgc
    18961 ggaccgctgt ggttcgagcg ggatcgcagg gtgctggtgg cgacactgga agaggtgctg
    19021 aacacaacca ccgagtgcga gattgaggtg ggctgttacg acgaacatgg tacagaacac
    19081 gtctacctgt tgcgttgcgt gcccgaattc gatgccgacg gttatgccac cagtgtgctc
    19141 accgtcgggc gggaaatcac gtccctcaaa cacaccgaat acgaaatgct gcaatcgcac
    19201 ctgcgtttgc ggcaactggc catccgtcgc gacgacaagc atatggaatc ctcgcggcgc
    19261 atggccgaat cggtgcatga aggcatcggc cagtgtctcg cggcattgcg catgcaattg
    19321 tctgccatga acgggatcga tgcaccctgg cagcagcgtg tggccacgat gcatggcctc
    19381 gttgatgatg ccattgcccg catgcgcgat gtgacccttt cgttgcgtcc caaggcgttc
    19441 gatctggggg tgaagagtgc ccttgaatgg atgtgcgggc gctttgcggc acagtacgcc
    19501 gtgggctgcg atgctgtggt ggacacatca gcaagccaga tgggcgatac gtgcgtgcgc
    19561 gtgatttacc agatcaccga aatcgcgctg gacaatattg cgcgccatgc cgacgcccgt
    19621 caggtacata tcgttctcga cagcgttgac ggacgttacc ggctcaggat cagtgacgac
    19681 ggcaaggggt tcaatattct gcagcgcagt gctgcccgtg tgagcgaact cgaatgggct
    19741 caggagcagg tggctggcct cgggggggag ttcgtggtgt gcagccagcc gggtcagggc
    19801 accatggtcg aagcgctgtt gccgggggta ggaaagtcct gacccggctg tgccgttcgt
    19861 ctgacaggaa aatcaagaat gcctgatggt tccatttgtt tgtcatgggt aatctgtgtt
    19921 ccatgcagca gtcaagcaaa tggaggcaga agtggcagac agcaggcgtt ggatcagcga
    19981 aaactggcaa gtgggcctac cgggcctgat tggagtgacg ggactgttcg gtgccgcagg
    20041 caatgtgtgg gtggcgctgc cagcagcatg tctggttgcg ggcaccaccg tctgggccat
    20101 gtatcgccag caacgcgagc ggcgggcaca tcgtgcagac attgagtcct ggatggaaag
    20161 catggccggc atggaatcgc tggccggggt gtgggcgcgt caggtcgaca cgggccgctc
    20221 gcacgtagag caggcgaccg ggcaactgac cggtcggttc gctggcatcg tcacccaact
    20281 ggatcagacc gtgcaggctt cgtcgctgtc gcaggggagt gatcagggcg tgcatgaagt
    20341 gttccagcgc agcgaacagc acttgcagaa aaccgcggac accctgcttg aagtcatcgg
    20401 tcatgccgaa cagttgctgg tcgaagtcgg caatctggtg ccgtttaccc gcgagttgca
    20461 ggaaatggca ttgatcgtgg cagatattgc gagccagacc aacctgctcg cactcaatgc
    20521 agcgatcgag gctgcgcgtg ccggtgaggc ggggcgcggt tttgccgtgg tcgccaagga
    20581 agtccgcatg ctggccggca agtcgggcga ggccggaaag cgcatttctg agaccagcaa
    20641 actgatcggc gaggcaatca gttctgcctg gcagaccgca caaagtaatg ccgagagcga
    20701 ccggcaatcc ggtacacagg ttcgcaacac catccgcagc gtgctcgacg atctgcaggg
    20761 catgaccgcc ggtctggccc aggccgccga tgcgttgcgc gattcgagcc ggcacatcca
    20821 ggccgaagtc gccgattcac tggttgcatt acagtttcag gaccgggtga gccagattct
    20881 ggttcatgtg accgacaaca tgaaatcgct ggaagcacac catggcctgg gcgcacaggc
    20941 ggaagagggc gcgaccttgc gcccggtgga ttacatgatt ctgctggaga atctggaacg
    21001 cgattacgca acgatcgaag aacgcaacaa tcatcttggg gcaaatgaag ctgcccaggg
    21061 tggcgaaatc acatttttct agaaaagggt gcaaccatgg caaaaaccat catgatcgtg
    21121 gacgattcgg cgacactgcg ccaggtggtg tcgatcgccc tcaagggcgc aggttatgac
    21181 gtgctcgaag catctgacgg caaggatgcg ctggcccggc tgaccggaca gaaggtccat
    21241 ctggtgatca gtgacgtcaa tatgcccaat atggatgggc tgacgttcgt gactgaaatg
    21301 aa
//
LOCUS       DAIANL010000125         4577 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1187336, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000125 DAIANL010000000
VERSION     DAIANL010000125.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187336"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          951 a         1483 c         1246 g          897 t
ORIGIN      
        1 gctggtcggc tcttcgatgg cgtagtacgt ttcgtcgtcc accttgaaac gccccttgca
       61 caacgtcgga taaccggcat tctcgtcatc ggcatagcgg tcgatgagca cgccgttgag
      121 gcgcgactcc atccccagcg aagttttctc ccaacgtacc gccctggccg gcgaacatac
      181 cccggcggta ttgggtgact cgccggtcac ataggacgac aacgcacagc ggccttccac
      241 catgacgcac aggctgccaa agccgaacac ttcgatttcg accggtacat tcgcccgcag
      301 gtgcgccacc tgttccagcg acagcaccct gggcagcacg gcgcgactga tgccatattg
      361 ctcgtgatag aaacggatgg cctcgtaatt ggttgccgat ccctgcaccg acaggtgtct
      421 gcgcatcgtt tcgtgacggg tggcggcgta ttccagcagc ccgacatcgg cgagaatgat
      481 ggcgctcacg cccaggtctg cagcagcatc gacggcattg caggcagtct gccagccgcc
      541 atcgcgcgga taggtattga gcgccagcag cacccttgca ccggcggaac gcgcataacg
      601 cacaccctcg cgcgcgctgg ccagatcgaa attcagaccg ccgaaattac gcgcattcgt
      661 cccatcgcga aaaccgatat agacacaatc ggccccgtgg tcgaccgccg ccttgagcgc
      721 taccaggcta cccgccgggc acaccagttc catttttttc tgcaacattg tccaggcttt
      781 ctgcgtatcg cactggtcag gtcctggtca cccatgcagc attgccggac gccggtgcgt
      841 gaaaatgagt tgaagatatt ttgatcctgt aaaactaaca taccctcccc ggtcagacaa
      901 gcgtccatac atcaagtgaa ccacaatatg ttgacaatac gctgatgatt tccacacagg
      961 cttggcccgg ccgcaacggt tccggtaagg gtttgtgtgc gtcgaagcca tgccacatgc
     1021 gtctgcaata cgagacaacc tgttgttggt ttcaggcaac aaggtaaact attaactcga
     1081 tctttttatc acctttgatg acatgcatgc gaaatttggg taaccttgct ccattccggt
     1141 tcaacgcgcg ccacctcttg accggtcaac agaagatctg cctgttttca aggagttaag
     1201 atgcagaaca ataccgcatt gcgcatggac aacggcccat tcaaactgct cgacgaaacc
     1261 agcatccgca acgccgtgct gcggcgcgac ccatacgatt attcctatgt ggaaaacgcc
     1321 atcgacgcat cgcacaaaaa cgcggttctg gccgatgcgc cgcagattcc ggatcgcggc
     1381 agctacgggt tgcccgacct gcgctacggc ccccgcttcg gcgaagccat cgatgacctg
     1441 ctcagcgtgc gttttcgcag catcgtcgaa gaaaaattcg acaccgacct gagcaaccgc
     1501 ccccccgtca tcgtcatgat gggcaatacc accggtcact acaacgaggg ctatgcgcat
     1561 accgactcca ggcacaagat cattaccgtg ctgctcggtt tcacccgcga atggccctac
     1621 gaaaaaggcc gtctgcgcat catgcgcagt tccgaccgcg aggatttcgc attcgaattt
     1681 gcgcccgaat tcggtcgcat gatgatgttc cgcgtctgcg accattcctg gcacggcttc
     1741 ctgccgcaga agggccagcg catgagtctg caactgtgct acgtcgattc cgacctgtac
     1801 gtgcagaaag aatacgtccg ccaccgcgtg agtgcgttcg ccaaatcggt accggtactg
     1861 cgcaaggtca tcgagtgggc gccgcgttga acgcttcgtg acatgccgtc gatcaacatg
     1921 ccgaggatga aatgagtttt ccgagagttc ccgaagtttc cgccgtggtc gagcattttg
     1981 gcaacgcgct gcgtgcagcg acaacagacg acttcccgta tcgccactgg ctgctttccg
     2041 acatgatccc ggagagcctg gcgaccgccg tactgatcct gcccattgcg ccgccactca
     2101 tcgatgagtg cggaggagtg cgcgacctca acgacaacaa cagcaagcgc agtttcttca
     2161 cgcccgaact gcgcgcgatg tttcccacct gcgaggtgtt cgcccaggcc atgcaagcgc
     2221 cggaaattgc ccgtctgttc ggcgaaacct gcaatatcga cgtggaaggc agctatctgc
     2281 gcatggaata catccaggac accaacggtg cctggctcga accccatcat gatgtgccgg
     2341 aaaaaatgtt ctccctggtg ctctacctgt gtaccgggcc ggaagccagc gaatggggta
     2401 cggacatcta tgacaaccag cggcgttggg tgggacgcag ttcagccgaa ttcaattcgg
     2461 cggtcatctt cgttccgggc gacaatacct ggcacggttt tgaaaaacgt ccgattcgcg
     2521 gcgtacgccg cctgatggag atcaattacg tcaagccaag ctggcgcgac cgcaaccagc
     2581 tgtcatttcc tgaccaaccg atcacgctgt cgaaatgaat gccatgctcg attccaatac
     2641 cttgttgact tcacgcaaac ccggtaccga cccgaacaac gtgcgcatcc gcagcttcgc
     2701 cggtacgcac gccaactcgg aacattacat cacggtcgag gcacctgccg gcctgccatt
     2761 tgctgctcag ctcgaattcg tcgaacagcg ctatgccgct gtggtccatg cgctcggtct
     2821 gtctgaagcc agtgcaatgt tccgccgcgt ccatctgagc gacatcctca accaggccgc
     2881 cgcagtaagt gccagccggc tgggcgacca caacgaacag tatccggtgg cggtttcgct
     2941 ggtacagcag gcaccgatgt ccggcgccaa gatcgcgctg ctcgcccacc acgtggaaga
     3001 cgccgaacaa catcccggcc agcgccagaa tgacggtcag atcgtgtttc accgccatgg
     3061 cctgcaacac ctgtggagca cgcagctgtg cgccggttcc gacagcgtgc cgccgacccc
     3121 ggccgaccag cagaccacgc tggtctttga ccagctgacc gatgcgctgg cacgtctgga
     3181 tggcaccctg ctcgaccact gtgtgcgcac ctggatctac ctcaaggatg tagacgtgta
     3241 ctacaaggac atggtggaaa gccgcatccg cctgttcaac cagcatggtc tcacccgcga
     3301 cacgcattac atcgccagca ccggtatcga aggtgcctgc agccaccgtg ccgacctgat
     3361 catgatggat gcctattcca tcgtcggcct gcaaccggag cagatgtcct ggctcaacga
     3421 cttcgaccgg ctgtgctcgg ccggagatta caacgtcacc ttcgaacgcg gtacccgcat
     3481 cgcctggcgc gaccgctcgc accacttcat ttccggtacg gccagcatcg ataccgccgg
     3541 cgaagtggtc caccccggcg atgtgctgca gcagctggat gtggcgctgg agaacgtcga
     3601 tgcgctgctg cgctccggcg gatcaacgct cgacgacatg atgtacctgc tggtctacct
     3661 gcgcgaccct tccgaccacc aggcggtacg cgcccggctg acggagcggc ttcccaatct
     3721 gcccattgtc atcgtgcaag gcccggtctg ccggcctgag tggctcatcg agatcgaggg
     3781 cattgccatc gctgccgagc gcaaccccac actgccagaa ttctgacctg acgacgcagc
     3841 agggtcgctg caggctgccc tgcggccagc cgcgccccgc agcaatacaa ccacatcatg
     3901 gtattgacag caaaatcggg taatatctgc ttcagccgtt cattccggta tacgcaagtt
     3961 acacagggct gaaccgtgcg cctgtttatc cgggaacgag cgcggctgtt tttcacaaca
     4021 tgacgttcga ggatattgca tgccgacatt attcgatccc attcaaatgg gcgacctctc
     4081 cttgcccaac cggatcatca tggccccgct cacccgttgc cgtgccggca ccggcagagt
     4141 acccaccgcc ctgatggcac gctattaccg ccagcgggct tcggccggcc tgatcatcag
     4201 cgaagccacg tctgtcagcc cgatgggcgt gggctaccct gataccccgg gcctgtggaa
     4261 cgaagcacag gtggctggct ggcaacaggt cacgcgtgaa gtgcatgcag ccggcggccg
     4321 gatttttgcg caactgtggc atgtcggcag gatttccgat ccgtactttc tcgacggcgc
     4381 cctaccggtt gctcccagtg ccatcgccgc caaaggtacc gtcagcctgc tgcgtccgca
     4441 acgcgaattt gtgacgccgc gcgcgctcgc taccgaggaa atctccggca tcatcaccgc
     4501 ctatcgccag gcagcgattc atgcccgcga agccgggttt gacggtgtgg agatccatgg
     4561 cgccaacggc tatctgc
//
LOCUS       DAIANL010000126         8502 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_164054, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000126 DAIANL010000000
VERSION     DAIANL010000126.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164054"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1755 a         2815 c         2297 g         1635 t
ORIGIN      
        1 atgccggtct gcacttcgtc aagcatcagc agccagccac gggcatcgca caattcgcgc
       61 aggccgcgca ggtaggcggc gtcgggcaca cgcacacccc cctcgccctc gaccggctcc
      121 accagcaccg ccaccacgtc atgattgtgt tcggccacct ggcgcaccgc ctccacatcg
      181 tcgtaaggca cacgcacaaa accggtcagc agcggttcga aacccgcctg caccttgcga
      241 ttgcctgtgg cggtaagcgt agccatggtg cgcccgtgga acgatttttc catcaccacg
      301 atggtcggca gcgcaatacc cttgccgtgg ccgtacaatc gcgccagctt gattgcagcc
      361 tcattggctt cgcaaccgga attgcagaaa aacgcccgat ccatgccgga aatgccgcac
      421 agtttttctg ccagctgctc ctgacgggga atctggtaga cattcgaggt atgaatcagt
      481 tgccccgcct gttcgcaaat cgcctgcgtc agacgcgggt gtgcatgtcc cagcccgttg
      541 acggcaatgc ccgcaagcgc atccagataa cgcttgccat tctcatccca cagccagacg
      601 ccttcgccac gaacaaacgc gacattctgc cgcgcatagg tattcatcag acacgacatg
      661 ttggtaaccc gcatccctca aagatcatta tcggtctgcc taaaaccaaa gcaaaaggcg
      721 gcttgcgccg ccttttgctt gccgccacgc aaccgcgctc aggcagagaa attggcagaa
      781 acaaacgtcc agtttaccag attccagaac gcctcgacat acttcggccg cgcattgcgg
      841 taatccacgt aataagcgtg ctcccacaca tcgcaggtca gcagcgcttt ctggccggcc
      901 gtcatcggcg tggctgcgtt gctggttgac acgatttcca gttcaccagc ggcatttttc
      961 accagccagg cccagccgga accgaacgtg cccacagcag ccttggaaaa ggcttccttg
     1021 aatgcatcga acgagcccca tttggcattg atcgccgctg cgagcgcgcc ctggggcgct
     1081 ccgccaccat tggggctcag gcaattccag taaaaggtgt ggttccacac ctgagcagca
     1141 ttgttgaaca cgccgccgga cgacttgagg atgatgtctt ccaggctcat cgcctcgaat
     1201 tcactgccct tgatcaggtt gttgaggttg gtcacatagg tctggtggtg cttgccatgg
     1261 tgatattcga gcgtttcagc cgaaatgtgc ggttgcaacg catccttggc gtagggcaaa
     1321 gcaggaagtt catggatcat atcgacactc cgttcatggt tccgtgaaaa gaccaagcta
     1381 ttctatcagg tatcccccgg cttacaccat gcgtccatgc catcccgcac gcacatacac
     1441 cattcgggta agtgcaacgc ggcgatagtc tgctagaatt tgcgcgttcc acttcaggca
     1501 gaccgccagc catgtcagaa ctgaccagca atgaaatcgt cattcaggga gtgaccacat
     1561 cgggacgcac attccgcccg agcgactggg ccgaacggtt atccggctcg ttgtcgacgt
     1621 tcggcgagga tcaccgcatg aattattcgc cgcatgtccg tccgatgacc attgacggcg
     1681 tcaaatgcgt catcgtggag aaggtgctgc aacagaagca gcccgccaca ttttctttcc
     1741 tgatgggatt tgcgcgcgac aatgaccttc gcgtgatcta tcccggccag acagaatcct
     1801 gaggcttgaa cgcccgcggc tccgcagttg cggagccggg acagcagatg tcattcgcag
     1861 gtgcccgcgc atgcccgggc accggattcc acctgtttac tgaggcatgg ccttgacagc
     1921 agccgacagg cggctcttgt gccgtgcggc cttgttcttg tggatgatct tcttgtcggc
     1981 aatgcgatcg atcacgctca tcgacgagga aaaggtgctc gcagcttcag ccttgtcgcc
     2041 agccaggatg gccttgcgca cttttttgat tgccgtacgc aggatggaac gcatggcggc
     2101 gttgcgagcc cgcaatttgt tgctttgtct ggcgcgcttt tctgcctgtg cgctgttggc
     2161 catattcggg tactcctgta tcagtccggt gaaaaccgct tattatgtca ctgagccatg
     2221 caacgcgcaa gcgtatcggg ccagcgcatg tgtctcaaat cgaatttccg catgcagcac
     2281 aacttcggga tgaccgtcag ccgtcctcaa tcgaacggat gacccgcgcc ggatttcctc
     2341 cgacgagcgt gttcggtgga acatccctgg tgactaccga acctgccgcg acgaccgaat
     2401 tctcacccac cgccacgcca ccgatgatgg tcgcgccggc agcgatccat acccctttct
     2461 caatcacgat cggcttgccg attgtggcgg tgcgtcgttg cgaagggttg agcggatgac
     2521 tcgccgtcat gaggctcacg ttcggcccaa tcatcacgtc gtcagcgatg ttgagtccgc
     2581 ccagatcgta aaacgtacag ttctgattta tgaaaacatt ttttccgaca gtgatttcat
     2641 ttccaccggc agcataaaag ggaggaatca ataagaaacg ctcatccacc gttttgccga
     2701 taagttcgct gaacaggagt ctgatttcat ccatatcgtc gaatgtcagc cggtttaatc
     2761 tggcggtgat cgccatcgac cgcctgacat ccgccaacat ggccgctgat tccggcgttc
     2821 tgctgtaaat tatcctcgca tgcttgtcat tcggcattcg ctacattccc ccctgacgcg
     2881 gcaaacagac gtttcagcgc acgagaacag cgtcggtatc cgccgaatgc agccagcgag
     2941 ccagagcgac cggatccatc aatcccccga atgcaatcga atcctgtcag gccaaccggt
     3001 ccaggccacc catccatggc cgcaacgcat cgggtaacac cacgctacca tcggcctgct
     3061 gaaaattctc cagaagggcg accagcgtgc gaccgatggc cagccccgag ccgttgagcg
     3121 tatgcagcaa atcgttgcga ccctgcgcat ggcgatagcg ggcctgcatg cgccgtgcct
     3181 ggaaggcgcc aaagtgactg caggaagaaa tctcacgata ggtgccctgc gccggcagcc
     3241 agacctcgag atcgtaggtc ttggcagccg aaaaacccat gtcgcgcgtg cacagcagca
     3301 tcttgcgata aggcagttcc aacgcctgca acacggcttc ggcatgacct gtcagttcct
     3361 cgagcgcatc ctcgccctgt tccggttcga ccatctgcac cagttccacc ttgtcgaact
     3421 gatgctgacg gatcatgccg cgtgtatcac gtccgtaggc cccggcttca gaacgaaaac
     3481 acggcgtgtg cgccacgaaa cgcaacggca acgtctctcg cgcgacgatc tgttcgcgca
     3541 ccatgttggt caccggcact tcggcagtcg ggatcaggaa catgcgttcc atcccggcac
     3601 gcggcacctg gaacaggtca tcctcgaact tgggtaactg gccggtaccg aacatggcgg
     3661 cggggttgac cagatagggc acatacacct cggtataccc atgccggctc acatgcagat
     3721 cgagcatgaa ctgcgccagc gcacgatgca agcgtgccat cggcccacgc agcagggaga
     3781 aacgtgcgcc ggcaatacga ctggccgttt cgaaatcaag accaagcggc tcgccgacat
     3841 cgacgtgatc gcgcggtgca aatgccatca caggcgactc accccaccgt gccacttcga
     3901 cattgtcggc ctccgaatca ccaaccggca ccgaaggatg cggcaggttg ggaatgcccg
     3961 caagcagggt ttccagatga ccttgcaccg cttcaagcgc tgtttccgcc gccttcaatt
     4021 cgtcgcccag atctgccacc cgcgccagga tgggcgccac gtcctcgccc ttcgacttgg
     4081 cgataccgat ggcgcgcgaa ctgctgttgc gctccgcctg cagattctgc gtgcggatct
     4141 ggatcgcctt gcgctcggcc tcaagccggg aaaacccttc cgcatccagg gaataaccgc
     4201 gcgtagcaag cctgcgcgtc acctcatcga gatcattgcg caaccattga atgtccagca
     4261 tgaaccgttc cgtccgtcat ccgcggcggg ccgaaacccg cgcataaaaa atcgccgcaa
     4321 gaaaatacac cgtcgtcagc acaatttcca gccatgccag tcgctgcgac aagccatgat
     4381 ccgaccagcc gcgtaccacc gcttcagcaa aatacaccag aatcatgagt gaagaccact
     4441 gataggtgta acgtttgccg tgcaacacgc caaacagcgg gaacaacagc ggtaccgcct
     4501 tgaaaatcag cgccgaaccg ccgggacgca gcggcgcaag ccagccttcc cacagcagcg
     4561 acagcacaat cagcccgatc aggcttacaa tcgccgtata ctgcaaccca ttctccagcg
     4621 tccgcttcat gacagccccc cctgcagacg caacgccgtt tcggccagac gccgccccag
     4681 tgcctgcgcc agctggcgtt catgctcgct cagcggcacc tgcgcacccg atccggccac
     4741 gtggcttgcg ccatacggcg aaccaccacc gacagtctgc gccagcgcag cctcgctgta
     4801 ggggatcccg accagcagca tgccatggtg caacagcggc agcatcatcg atagcaaagt
     4861 gctttcctga ccaccatgct ggctggccgt ggaagtgaac accgccgccg gcttgccgct
     4921 cagcgcacct gtgagccatt cgcccgtggt gccatcaaga aaatgcttga gcggcgctgc
     4981 catattgcca aatcgcgtcg ggctgcccag cgccagtgca caacactcgc gcaagtcgtc
     5041 ggcggtgaca taaggtgctc cttcgtccgg gacggccggc tgcgccacct gcgtcacgct
     5101 ggccacctct ggaaccgtgc gcaggcgcac agcgatgccc ggcagcgatt ccgcaccgcg
     5161 cgcaatcgac tgcgccagag cgcgagtcga accatgcctg ctataataca caatcagaac
     5221 ttcacgcatg cgattctgca gccttcacca aagcgccgta ttataccgtc cgattcccgt
     5281 taccgcacgg attcgccaat acgcagccac aggatgatca ggcgcaaccc ggatgatcag
     5341 gcgcaaccct aggatgatca ggtacagctc catgaaaaaa atctggcatt tcgccatgct
     5401 ggtggtacag cgtttccatc tcgacggttc cggcaaaaca gccgccagcc tgacctacac
     5461 ctccctgctc gccatcgtgc cgctcatcgc gctcggactg agcatcatct cggcgtttcc
     5521 tgcctttgcc cagatcagcg ccagcttcaa gcaattcctg ctgcagaaca tggtccccga
     5581 taccgcaggc cggatgatcc gcggctacat gctgcagttc tcgcaaaacg ccggcaagct
     5641 cacgctgatc ggaacgctgg cgctcggcgt caccgcactc atgctgatgc agaccatcga
     5701 gcacgccctc aacggaatct ggcatgtgcg ccgcaagcgg ccgtgggccc agcgtttcct
     5761 catctactgg gcggtgctca cactcggccc catgctgatc ggtgccagtc tgtacgccgc
     5821 ctcgtatctg gccgggctgg cgctcggtct cggccgggac accagcaccc tgcatgtgct
     5881 cggcctgcaa ctgactccgg cactgctcac tgtcagcgca ctggccatgc tgtacctgac
     5941 cgtgccgaat cgctacgtac cggccgctca cgcctggatc gtcggtacgg tgggcggcct
     6001 gtgtttcgag gggatgaagc tgctgttttc cggctacatc agccatttcc cctcctacac
     6061 catggtctat ggcgcatttg ccgccctgcc actgttcctg ttgtggatct acctgtcctg
     6121 gatcacggtg ctgaccggcg cgaccatcgc cgccaccctg ccctatttcc acaatccgca
     6181 gctggcacac gacaccagcg ccggcagcct gttccacacc gccctgctca cgctggaacg
     6241 tctcgcccgg gcccagttgc agggcgagac cgtcaccctg cgccagctcg ccggcgcaac
     6301 gcaagccaac tgggatgtgc tcgaacatgc tctcgatcac ctgactgacc ttcactgggt
     6361 attgcgcagc ggcaaaggct gggcactggc catgcgcccc gacgacatcc ccatacgtca
     6421 gctgttcgac acgctggtct ttcagccaca tgcgcattca tgcacgcttg acgatctggt
     6481 cagacagcca cagttgaatc tgaccgactg gatacggcaa cgctcgcact agccggccct
     6541 gccgcgattt atcccgcgcc gcccccttcg ccgatgccat gcgcggcatc cagcaccagg
     6601 gcgtcaaact cggtagccaa catcaggccg cgctccgcaa cgatctcgcg caccggtcgc
     6661 ccgctgcgtt ctgattcctt ggcaatctcc gcagcccggg catagccgat atgcgtattg
     6721 agcagggttg ccaagccaac gctggcatgc acgaactgcg cgcagcgatc gcggtgcacg
     6781 atcaggccgc gcagattctt ttccgttgca gcgtcgatgg cccgggtgag ccagtccatg
     6841 gcatcgaaca gggccgaacc gagcgcaggc atcatgacat tgagttcgag ctgtccggcc
     6901 tgggtcatcc acgccacggc agcatcctga cccagcacct ggaaacagac ctgattaagc
     6961 atttcaaaca tcaccggatt caccttgccc ggcatgatcg acgaaccagg ctgcaccgcc
     7021 ggcagggtga tttcgcccag cccggtacgc ggtccagaag ccagcagacg caggtcgttg
     7081 gcgatgcgcg tcagttcgag cgccaacccg cgcagcgcgg cagataccgc ctgcatgtcg
     7141 tacatggact gcatctgcgc cgccagatcg ccaacagtga tcggttcgcc ggtcagttcg
     7201 gcgagttccc gcgccacctg ttgcgcatag tcaggactgg tattgagccc ggtgccggca
     7261 gccgagccac ccagtcctgt ggcacacagc aacggcctcg tcgcggccag ctgcgcggct
     7321 gcgcggcgca gggtccaggc ccaggcatcc atttcgcgtc ctaccgtggt cggcacggca
     7381 tcctgcagat gggtgcgacc gcttttgacc gtgtcgcgct cgcgcgcgcc aagcagcgcg
     7441 aactgctccg ccatgcgctc gacagccgcc agcaggcgtg gcagtttggc cagcaacgcc
     7501 agacgaatgg ccgtcggaat ggtatcgttg gtggactgcc ccatgttgac atggtcattc
     7561 ggatgcaccg gcgcatagct gccaggtacc ccgcccagca tcgtgttcgc cagattggcg
     7621 atcacttcgt tgctgttcat gttgtggctg gtgccggcac cggcctggaa acggtcaacc
     7681 acgaactgat ccatgaattc gccattcaaa atccgctcgc aagcggatac aatggcatcg
     7741 cgtcgttcgg catcgagcca gccaggctgg cagttgaccc gggcggccgc cagcttgatg
     7801 cgcacatgcg catgcacgaa atcggggtct ggtccccggc cggaaatgga aaaattgtcc
     7861 agcgcgcgcg cggtttgtat accataccac gcgttcgccg gaaccctgta ctcacccaaa
     7921 gagtctttct cgattcgaaa ctcagtcatt ttcgacacgc ccgacaaaaa ccgcaattat
     7981 aggagtgatt tcatgttgaa acgagtcctg gcctttatcg tgctcgcaac agtctgcgcc
     8041 agcgcctggt cattcgacgt taccgacacc cagggccacc gtcatacgct ggccgattac
     8101 aaggggaaat gggtattggt caatttctgg gccacctggt gcccgccgtg ccgtgccgaa
     8161 atccctgatc tgattgcact gcataaccag tatcgcaaca gcaagctcga agtgctcggt
     8221 atcgccatgg aatacaacga cccgaacgaa gtcacccgtt ttgcgcagag catgcacatc
     8281 agctatccga tcgtgctcgg caacgacgcc atcgccagcc agatcggcca ggtacaaggc
     8341 ctgcccacct cttacctcta taacccccag ggcaagatcg ttgcctacca ggttggtgca
     8401 ctgaccaggg aggccgtgga acattacatc aacacgtcgc acgcgccagc cgcaccccgg
     8461 aaatagccct gatcagaccg tgccactcca ccatgcggct ag
//
LOCUS       DAIANL010000127        16341 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1992692, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000127 DAIANL010000000
VERSION     DAIANL010000127.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 16341)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16341)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16341
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992692"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3270 a         5450 c         4631 g         2990 t
ORIGIN      
        1 tgttcggcct gggcacggcg atcatccagg gaacgctcgg tgcgctgttc ggccgcatgg
       61 cccagcagca tcggctcaac gagcagcaga tgcgcacact ggcactggaa acggcttcgc
      121 gcacgctgac ctggggcgga gccgcctacg tcgccgccgg catatccagc ctgatatggc
      181 cagggctatc gcgctggcaa ctcgacaccg gcatcgccat ccgcggcctg aaccagatcg
      241 acctgccgct cgtactcgcc atctgcaccg tcgtcggcat cggcctcggc ggcttcgccc
      301 tgcggctgca tgcactgatg cgcgcgcaac catcgatacc cacctcttga atccgcgctc
      361 ttcgtcccca tatcgggcgc atcgattcac ccgcaacgat ttaattgcaa cggagccgta
      421 ccgatgaatg caactgcaca aaaagaaacc ctcggatttc agaccgaagt caagcaactg
      481 ctgcaactga tgatccattc gctgtacagc aacaaggaaa tcttcctgcg cgaactgata
      541 tccaacgcct cggacgccgc tgacaagctg cgcttcgaag ccctgtccga caacgcactg
      601 tgggaaaatg acaccgagct gctcatccac gtcgacttcg acaaggaggc cggcaccatc
      661 accatccgtg acaatggcat cggcatgacc cggcaggaag tcatcgacca catcggtacc
      721 atcgcccgtt cgggcacgcg ccagtttttc gaaaacctca ccggcgatca ggcgcgtgac
      781 agccacctga tcggtcagtt cggcgtcggc ttctattcgg cgtttctggt ggccgatcgc
      841 gtgacgctga ccacccgacg cgccggcctg agcgccgagc atggcgtacg ctgggaatcg
      901 gctggcgaag gtgaatacac cctggagaat atcgaccacc cgcaacgcgg caccgagatc
      961 gtcctgcatc tgcagccgga caacgacgcg ctgctgtccg aacatcgcct gaaagccatc
     1021 ctgcgcaaat attcggacca catcaccctg ccgatccgca tgcgcaagga agaatggaac
     1081 gcggacaaac aggaaatggt gttgctcgac gaactggaaa ccgtcaacca ggcgtccgcg
     1141 ctgtggaccc gtccgcgcaa cgagatcagc gccgaacaat atgatgaatt ctacaagcac
     1201 ctgtcgcacg atttcgaggc gccgctcgca cacgtgcatg cccgggtgga aggcaaacag
     1261 gaatacaccc agttgctgta catcccggcc cgtgcaccgt tcgatctgtg ggatcgcgaa
     1321 caacgccacg gcatcaagct ttacgtacgc cgcgtattca tcatggacga tgccaaacag
     1381 ctgatgccgg tctacctgcg tttcgtgcgc ggcgtaatcg attcgaacga cctgccgctc
     1441 aacgtctcgc gcgagattct gcaggaaagt cgcgacatcg atgcgatccg cgcaggatcg
     1501 gtgaaaaagg tactcggcct gctcgaagac atggcaacga acgacgcaga aaaatatgcc
     1561 cgtttctggc aggaattcgg ccaggtgctc aaggagggcg tgggcgagga ctacagcaac
     1621 aaggagcgca tcgcgaaact gctgcgcttc gcctcgaccc gcgacgacac cccgaatccg
     1681 acggtcagtc tggcggacta tgtcgcacgc atgaaggacg gccaggacaa gatctactat
     1741 gtcaccgcgg aaaaccatgc cgcagcgaaa aacagcccgc atctcgaatt tttccgcaac
     1801 aagggggtgg aagtcctgct gttgtccgac cgggtcgacg aatggatgct gtcctacctg
     1861 accgaattcg acggcaaggc gctgcaatcg gtcgccaaag gcagccttga cctcgatcag
     1921 cttgccgacg agcaggaaaa agaggaacag gcgcgtgaag cggacgaatt caaggacctg
     1981 accacccgca tccaggaagc actgggcgag cgggtcaagg aagtgcgggt cacccaccgc
     2041 ctgaccacct cgcccgcttg cctggtcgcc aacgagcatg atctgagcgg caacctcgaa
     2101 cgcatgctca aagccgccgg ccagaacgtg ccccgcagca agcccgtgct cgaaatcaac
     2161 cccagacacg gtctggtaca gcgactgcgt gacctgtccg ccggcgaacg tttcaacgac
     2221 tggagcctgg tgctgttcga ccaggcacaa ttggccgaag ggggccagct ggaagatccg
     2281 gccggtttcg tccagcgcat caacaccctc tggctggaca cccactgatg caccggcgcg
     2341 attgatgcta tcatcgcgcc tgcctgcggc acgctgcgct gcgacgggaa atcgtcgtgg
     2401 cgcaacgtgc ttcccgcccc cgtcacccac catcggcctg tccattcatg accctgcccg
     2461 ttgcagacac cctgatcgaa gcccgctgga tcattccggt cgaacccagc gcaaccgtac
     2521 tgaccggtca cgcgctcgcg atccgtgacg gacgcattct ggaaatcctg cctgtcgacg
     2581 aagcgcgccg gcgttatggt tcggcccggc gtcacgtgct cgaccagcac gcactgatcc
     2641 ctggactgat caacctgcac acccatgcgc cgatgaacct gctgcgcggc tatgccgacg
     2701 acctgccgct catggaatgg ctgaaagctc acatctggcc ggcagaaacc cgccatgtct
     2761 ccgccgactt cgcctgggac ggcactcggc tggcctgcgc ggaaatgctg cgtggtggca
     2821 tcacctgctt caacgacatg tactttttcc ccgatgcggt ggcagaagcg gctctggcca
     2881 gcggcatgcg tgccgtcatc ggtatggtgg tgatccagtt tcccactgcc tgggcgagcg
     2941 acaccgatga ttacctgcac aaagggctgg aactgcgcga ccgcatgggc gacgaaccgc
     3001 tgctcgggtt ctctctggcg ccgcacgcac cctacacgct cgacgacgcc ggcttcgaaa
     3061 aaattgcagc cctgtcggcc cagctcgacc tgccggtgca tgtccacctg cacgaaacgc
     3121 aggacgaaat ccgccacagc caggaacact accgccagcg cccgcttgca cggctgcatc
     3181 gcctgggtct gaccggcccg agtctgatcg cagtacacgc ggtacatctg gaagacgacg
     3241 aaatcgaact gctcgcggcc cagggctgcc atgtcggcca ctgcccttca tccaatctca
     3301 agctggccag cggcttcgcc ccggtcgccg ccatggagcg ggccggcatc aatcttggca
     3361 tcggtaccga cggcgccgcc agcaacaaca cgctggacat gtttggcgaa atgcggctcg
     3421 ccgcgttgct ggctaaagcc tgcgccggcg acacaaccgt gcttccggcg gccagggcac
     3481 tggaaatggc tacgctcggc ggtgcccgcg ccctcggcat gcaggatcgc atcggttcgc
     3541 tcgtgccggg aaaactggcc gacatgactg ctgtcgacct gtcggccctg aacacgcaac
     3601 catgctatga ccccgtgtcg caactggtct acagcgccgg ccgacaccag gtcagccatg
     3661 tgtgggtaca gggtcgcctg ctgctcgacg atggccgcct gacccagagc gacgaagcag
     3721 aaattcgccg catcgcccat agttggcgcg atcgcatcct ttcacgttca ccggcccgct
     3781 gaaaccccat gctacatacc gccatcgact ggatcgttca caccgtacag ggctggggct
     3841 atcccggcat cttcattctc gccctgctcg aatccaccat cgtacccgtg ccgtcggagc
     3901 tgatcctcat tcctgccgga tacctcgctt tccagggcaa aatgagtttg ccactgattc
     3961 tgctcaccgc cacgctgggc ggtctggccg gtgcatcgat caattacgcg ttcgcgctgc
     4021 tggtcggacg cccgttcctt gaacgctggg ggaaatattt cttcgtcgaa ccggccctgc
     4081 tgcacaagac cgagcgcttt ttccgcgact acggcgcaat ctcgaccttc accggccgac
     4141 tcgtcccggg catccgccac ctgatttcgc tgcccgcggg actcgcgcgc atgaaccgcg
     4201 cagcgttcgc actgtacacc atgctcggcg caggactctg ggctgccatt ctcaccatga
     4261 cgggttattt catcggcgac aagattgcgc tggttgaacg cgaactgcac ctgatcacgc
     4321 tgggcgtgat cgtggcttca gctgccctgt tcacgggtta tgtgttatgg cgccgacgac
     4381 tggagcggca agagtctgcc cgccgcgacg gtacgacctg atggccacac ggccggttga
     4441 cagacgcgat gggagtggcc ggatgcacga cacggccaca cagcaatcga agcatatcga
     4501 cggactggta taactgcgaa caccgccgta cgcagcacaa cgcggggttt cagccacctg
     4561 ctagtggcgg gtgctgccct cgccgccagt catcacgcca gcgtcgagcc agagctgtgc
     4621 cgcatcgatg gaatcgaaat gataactgtg gccgcagaat tcacactgca catcgacctg
     4681 accctgttct tccagcaccg aattgacttc ctgctgaccg agcagacgta acatatccgc
     4741 cacgcgctgg cgtgaacaac cgcagaaaaa ggtgagttcc tgcggttcga acacgcgcag
     4801 atcttcttcg tgaaacaggc gacgcaacac ttcggatgcg ccgagataca gcaattcttc
     4861 acgtgtcagc gtatcgagca gacggctggc gcggtcccag gcgtcagcat caccctgcgg
     4921 caagtccggc aaacgctgga tcagcacacc ggcggcctgc tgcgcatccg cagccagcac
     4981 gatacgggtg tcgatctgtt cggaacgcgt catgtagttc atcagcgcct cggccacact
     5041 gccgccttcg agcgccacga taccctgcca tgcctgctgc ccttcttcgc ccggatccag
     5101 tgtcagcacc agccggcctt cacgcagcga acgcctcatc atggcatcgt ccggttcgcc
     5161 ttcccatttg gccatggcgc gcaggcgcat cgatgactgg atttccacga ccagaagacg
     5221 tacagcgcct tcggactgca tttgcagcac caggctaccg tcaaacttga gcgtcgcaat
     5281 cagtagcgcg gcagccgcgg tcaactcacc cagcgcatcg cgcagcacgg gaggatattc
     5341 cacccggcgc agcacttcgc gccacgattc gtcgagatgc acgatctcgc cacgaatggc
     5401 ggcattttca aacaggaaac ggatcagact gtcaggggca ggcatctcgg aacacccggt
     5461 ttccggcact gaagtggcca gaagataagt atcctgcatt ccgggagcgc tgtccactcc
     5521 cggaaatccg catcgcgccc cgcacaggca atgcgccaca cggcgggggc ataattcaat
     5581 gcattccgga ttcgcgttac aaatccggat tcatgccaga tcagctcttg gccgaatagg
     5641 caaggcacca gcctttcggg ctgatcgatc cgtcaaccac cttgcagctg ccatcggctt
     5701 tggcagactt gccggggatg aactggatgc agctcgagca ggaagcggta cccttggggg
     5761 aatcctgata ttgcatggca gccttcgcaa ccttggcagc ataagcttca cggctgatga
     5821 agctcacagg cacggcagca acgccagcca gcagggtagc ggttttcaga aacgaacgac
     5881 gggaattctc catggtgaac ctcctggttg tggttgaata aaaacacccg cggccattat
     5941 aagcctgaac cctccccggt cgtccagatt tttttgagac aatgttgtct taatagcaaa
     6001 ccggcaacgg atcggggctg cgccagagca tccaggtagc caccgaacgc cacggatgcc
     6061 acattcagtg cgtttgcgca gctcgcacac cctgccctgc gagttcgggg caacggcgtt
     6121 ccagcgcctt gcgcagcctt acgtcggccg gcggcagcac atcgggacgc agcagattga
     6181 cggtgattaa ttctccacgg atcgcgttgc ggctgaaatc gtgatgatcg cgaaccggat
     6241 gacgcaggtc ggagccggga ctacgatgcc agggaatccc gcaaagcggg ggcgcgattt
     6301 caccccaacc cacaggggac gtggttctgg cgggcagcgt gctgcggtgc atttcttgtc
     6361 aagggggtca gccattgact gcggatcgcg ccttgcaatc catcccgcca gagcagcgtc
     6421 gcgaccgtgt gaaattaatc accacgtcct caagggacgc gcctttcatc agaatgaggt
     6481 gaacaggatg acggaagtcg ctacaatgga gcaaatgcac gtcaccgggc cgccacatgc
     6541 aatggatatc caccattccg ccactgtttg aagcctttct ggccacgata ctgctcggtt
     6601 tcgtgcttgg cctggaagta cacagctacc gccgcgccaa cgagcaggac ctgggcatcg
     6661 gcaccacccg caccatgacg ctgattgcaa tcaccgggtt catgctggcc gatctcgatg
     6721 cgcgtctaat gctgttcggc atcggatttg ccgtgctggg cggcctgctc ggactacaat
     6781 actggaaagc gctcacgctc ggcaaacaaa gcctgatgcc cgcgctgatc gccctgctga
     6841 cctacctgct cgggccgatc gccctcatgc acaacctgtg gctgctggtg ctgtacacgg
     6901 tggcgatcct gttgctgctc ggcgaaaaac ccggcatccg ccgcctgtct gaccagatgc
     6961 gcaccaacga gctggtcacg ctggccaaat tcatgatcat ggcaggcctg atcctgcccc
     7021 tgctgcccaa ccagcccatt gcacctgagc tcaacgtcag ctggaaccag ctgtggcttg
     7081 ccgtggtcgc ggtgtccggc atctcctaca tcagctattt cgtacagaca tatttcctgc
     7141 cacgttccgg gctgctgcta tcgggcctgc tcggcgggct gtattcgagc acggcaacca
     7201 ccgtagtgtt gagccggcgc gcgcaacagg accccatgtc cggtcagcgt tcctatgcca
     7261 ccgccgtcgt gctggcaagt tccatgatgt atggccgact gctggtgctg atcctgttgc
     7321 tcgggcacat tgtcatcgcc agccggttgt ggctgtcatt cgcgctggcc atcgccgcgg
     7381 ccgcgctcgc ggcctggatg ctacgcacaa ccacccccga cacgaagact gcggcgccac
     7441 ccgaaactcc gcccgtgcgt cacccgctcg aattctcaac cgccgtcgtc ttcgccggcc
     7501 tgtttgtcct gttcgccatc atcaccgaag tggtgatcgg gcgctttggc acgcacggac
     7561 tgcactggct ggcgtttctc gtcggattta ccgacatcga tccgttcatc ctggcgctgc
     7621 tggccggaca ctatggcctc gatccgcaaa tcatcgccca ttccatcctg atcgccaccg
     7681 ccagcaacaa cctgctcaag gccatttacg ccgtgctcat cacacgcaag gcaaaaatgc
     7741 tgcaggcagc gcttgcgctg gtgtccatca gtgcgctatc gctggcttat gcctaccttg
     7801 catgaacgat ttgcccgtgc aattactccc aaagaccctg caaccggaat agaatgatgg
     7861 ctgcagccgt accagcgggt tgctgtggct tgcctgacca ggagtgcaca tggaattcaa
     7921 ggactactat gccgtcctcg gtgtggacaa aaccgccagc gccgaggaaa tcaagaaggc
     7981 ctaccgcaag ctggcgcgca aataccatcc ggacgtcagc aaggaacccg atgccgaacg
     8041 caagatgcag gccgtcaacg aagcccatgc cgtgttgtct gacccggaaa aacgtgccgc
     8101 ctatgaccag atcggtcagg gttaccacga cggacaggat tttcagccgc ctcccgactg
     8161 gggttccgga ttcgaattca gcggccgcgg cttcggtggt gccgacatgc acgacgccag
     8221 cgactttttc gcgcagttgt tcggacgcgc cggacgtgcg cgccacagcc agcagatgcg
     8281 tggcgaagac cgccatgcca ggctggtcat tgacctggca ctggcgtgga atggtggcag
     8341 ccagaccgta acgctgcgcg tgcccgaatc gggcgaaacg ggccaggtca ctttgcgcga
     8401 acaaacgctc gaagtcaaaa ttcccaaagg cattaccgaa ggccagcatg tccgcgttgc
     8461 cggcaagggc agcccgggct acggcggtgg tcctgccggg gacctgtatc tcgagatccg
     8521 cttccgtccc gacccacgct tccgcgtcga ggcgcgcgat gtgtacgcca cattaccggt
     8581 caccccctgg gaagccatgc tcggcgccag tgtccaggcc accaccccct caggcgaagt
     8641 tcaggtcaag gtgccgccag gctcacaaac tgggcgcaag ctacgtctca aagaacgcgg
     8701 tattccaggt acgccgcccg gcgacctgta cctggtgctc gaagtcgtgc tcccgcccgc
     8761 cgtttcagca cgcgcgcagg aactgtatgc tgccatggcc agggatctgg ctttcaatcc
     8821 acgccaacgc aagggagaat gagcatgtcc agcgtgattc cgcccgacca ggacgtcaca
     8881 ttgcagctta ccgtactgca actggcacac gcctgccagg tgcagccaga ctgggtgatc
     8941 gagcgcatgc aggctggcct gctgtgcgac atgcccgtga ccgcagccgg atcgcaccgt
     9001 ttcgacagcc tgatcctgtg ccgggctcga cgcatgcgcg ccatagaacg cgacttcgag
     9061 gccaatgccg agcttgccgc cctggtagcc gacctgcacg agcagatcga tatgctccgc
     9121 ggccagctgc acctgcatac ccaccgctga gtccctgcag gtgacgcctg actgtcatgc
     9181 cgccaggcta tgcttgcgcc gttcggcccg cacacacgcc ccgttcgatg gagaatccga
     9241 tgccttccgt tccgctgacc gatagcgaac ttgacctgat caaccgttac tggcgagcag
     9301 ccaactatct ctcggtcgga cagatctatc tgcttgacaa ccccatgatc agaacaccgc
     9361 tcgaactggc gcacatcaaa ccgcgcctgc tcggtcactg gggcaccaca ccggggctca
     9421 acttcatcta cgcccacatg aaccgcgtca tctgccgcga cgacctgaac atgattttca
     9481 ttgccggccc cggccatggt ggcccggcca tggtcgcaaa cacctggctc gaaggcagct
     9541 acagcgaatt ttatccgcac gtcccgcgcg acgaaatcgg catgaaacac ctgttccgcc
     9601 agttttcctt ccccggcggc attccgagcc acgtcgcgcc ggaaacaccc gggtcgattc
     9661 acgaaggcgg cgaactcggc tatgcgctgt cgcatgccta cggtgcggtg ttcgacaacc
     9721 ccgatctcat cgcctgctgc gtcgtgggtg atggcgaggc cgaaaccggc ccgctggcca
     9781 ccgcctggca ttccaacaag ttcctcaatc cactcaccga cggcgccgtg ctgccggttc
     9841 tgcacctgaa cggctacaag atcgccaacc cgaccgtgct ggcacgcatc agccatgacg
     9901 agttggcgca actgttgtcc ggttatggct acacgccgtg gttcgtcgaa ggcgacgacc
     9961 ctgctgtcat gcatcggcaa atggccgaaa ccctggacca cgcgcttgcc gacatccgca
    10021 acatccagcg tcaggcgcgt gactccggcc agccatcccg cccgcgctgg ccgatgatcg
    10081 tgctgcgcac acccaaaggc tggaccgggc cgaaaaccgt cgacggcctg caaaccgaag
    10141 gcagctggcg ttcgcaccag gtgccaatgg gtgacatggc caaccacccc gagcacatca
    10201 ccatcctcga gcaatggatg aaaagctatg tccccgggga attgttcgac ggcaatggtc
    10261 ggctactccc ggaacttgca gcactcgcac cccagggcac gcgtcgcatg ggcgccaatc
    10321 cccacgcaaa cggcggcagc ctgttgcgcg acctggaatt gcccgactac cgcaattacg
    10381 cggtcacgct ggcccatccg gggaaaaccc aggctgaggc cacgcgcgtg ctggggcaat
    10441 atctgcgcga catcatgcgc aacaaccgcc agaatttccg cgtattcggt cccgacgaaa
    10501 ccgcgtccaa ccgcctcagc gcactgttcg aggtcaccgc ccgcacctgg cttgccgata
    10561 cgctcgcaag cgatgaccac ctggctgccg acggccgggt catggaaatt ctctccgagc
    10621 acacctgcca gggctggctc gagggctatc tgctgaccgg acggcatggc ttcttttcct
    10681 gctacgaagc cttcatccac atcatcgact cgatgttcaa ccagcatgcc aaatggctca
    10741 aagtcaccag ccagcacatt ccgtggcgtc atccgatcgc atcgctcaac tacctgctga
    10801 cttcgcatgt gtggcgccag gatcacaacg gtttctcgca ccaggatccc ggcttcatcg
    10861 atcatgtggt caacaagaag gccgacatca ttcgcgttta cctgcccccg gatgccaaca
    10921 ccctgctctc tgtcgccgac cactgcatgc gtagccgcaa ctatgtcaat gtcatcgtcg
    10981 ccggcaaaca gcccgcgccg caatggctga ccatggagca ggccatcgcc cattgcgcga
    11041 ccggcatcgg tatctgggaa tgggcaagca acgaccagaa cggcgcgccc gatgtggtga
    11101 tggcctgctg cggcgacgta ccgacgctcg aaacgctggc ggcagtggac atcctgcgcg
    11161 acctggcgcc ggaactgcgc atccgcgtgg tcaatgtggt cgacctgatg acgctgcaac
    11221 cggaagaaga acacccccac ggtctgtcgt cgcgcgattt cgacagcctg ttcacgatcg
    11281 accgacccat cattttcgcc taccatggct atccatggct gatccaccgt ctgacctatc
    11341 gccgcaccaa ccacgacaat ctgcacgtgc gcggctacaa ggaagaaggc accacgacca
    11401 ccccgtttga catggtggtg ctcaacgaac tggaccgctt tcatctggtc atcgacgtgg
    11461 tggaccgtgt cgctgcgctg gccgcccgtg ccgaacacat caagcaggcg atgcgctacc
    11521 gcctgctcga acaccagcag tacatccgcc tgtacggtga agacatgcca gccattcgcg
    11581 actggatctg gccgcacgga tgacagcatt ccctgtcatc ctcacggtca acagcggatc
    11641 gtcttcgctc aaggccagcc tgttccttac cgacggcact cggcacgact ttcgtttcga
    11701 gcatatcggc cacggttttc cgcatgacca ccgcgaagcc ttcgacgcgc tgatccgcgc
    11761 catgggcgac acacgaccca acgcaatcgg ccaccgcatg gtgcatggtg gcaatgtgac
    11821 cgatgccgcg cgacgcgtcg acgataccga acgcgaacgt ttgcagcaca tcgtcgcgct
    11881 tgccccatta cacctgcccg gtaacctgct cggcatcgac ctgtgccgcg aacgcttcga
    11941 tgtgccgcaa gtcgcctgtt tcgacacggc ttttcacgcc accctgccgg aactcgcctg
    12001 gcggttaccg ctgcccgagc gtttcggcct gcgccgctac ggatttcatg gcatcaacta
    12061 ccgccatgtg gccagccaac tgccggcgtt gctcggtgat gccgcacggg gaaaaatcgt
    12121 ggtggctcac ctgggcaatg gcgccagcct gtgtctgctc gacgacctcc agtcagtgga
    12181 caccaccatg ggctacactc cggccggggg catcccgatg ggcacgcgca gtggcgacct
    12241 cgatcccggc ttcatgctcg cgctcgcagc gcaactcgac accgaggcgc tgtcgcacct
    12301 ggtctatcac gaaatgggat tgctggccct gtcaaaccac gaaagcaacg aaatgtcgga
    12361 actgcttgcc agcgccacgc ccgctgcccg ttttgccgtc gactacttca gtcgccaggt
    12421 acgcggcgcc atcggcgcgc tggcctgcaa ggccggtggt atcgacgcac tggtgttcac
    12481 tggcggcatt ggcgaacacg cagcaccggt gcgccagtcc atctgtgacg gcctcgacct
    12541 gctcgggttg cgcctcgacg acctggccaa ccgggaacag cgtacccaca tcgggcagac
    12601 gacatcgaaa ccggtgctca tcgtgccagc cgacgaagaa ggcatgatcc accgactgac
    12661 gctggatacg atctactgat tcagcctggc gatcagcgtc tcaagcttgt cctcgtcggt
    12721 ggtacgcagg attttctgca ccagcaggac gatcttttgc atgtctgact tgaggatctg
    12781 ctgcttgacg ctgagcaggc tggccgactg catggaaaaa cgccgcaagc ccaacccgag
    12841 cagcagccgg gtcagccgcg ggtcgcccgc catctcaccg cacaccgata ccggcttgcc
    12901 ggccttttcg ccggcacgca gggtgacgga cagcaattgc agcacggccg ggtgcatggg
    12961 gtcgtagaga taggccacgc catcgtctgc gcgatcgatg gcgagggtat actggatcag
    13021 gtcattggtg ccgatggaaa gaaaatccag ccgttcggca aacgattgcg ccatcagcgc
    13081 cgcagcaggt acctcgatca tgccgccgac ctcgatgccc gggtcgaatg gcaggttttc
    13141 ctcgacaagg ctggccttgg cgcgcgcaat ggccgcatgg gtcagacgca gttcctgcag
    13201 gttgcacagc atcgggatca tgatcttgat gcggccgtaa tgcgacgcgc gcagaatcgc
    13261 acgcagctgg gtatggaaca tggcactttc ggacagacac aggcgaatcg cacgcttgcc
    13321 caacgccgga ttgagcgtga cctcggtcaa cccgcccagc ggcttgtctg cgccaacgtc
    13381 gagcgtgcgg atggtgaccg gcatgtcgcc catggcctgc gctacctcgc gatacgcctc
    13441 gaactgttcc tcctcgtccg gccagccact tcggttcatg aacagaaatt cgctgcggaa
    13501 caggccgata ccgctcgctc cggcctcctt ggccgccgcc acgtcgcgcg gcagttcgat
    13561 gttggcatgc aattcgatgt ccagaccatc gagtgtggcc gaacggctgc cgctcaggcg
    13621 cttgagcttc tgatgttcga tcacccactg cgtctggcgc aactggtatt cttcaagctc
    13681 ggcgtcgtcc gggtcgacga tgaccacgcc ctgttccccg tcgacgatga tgatctcgtt
    13741 gtcgcgaatc agcccgtgcg cattgtgcag cgccatgacc gaagaaatat tcaggctgcg
    13801 cgccaggatg gcggtatgcg aggtagcgcc acccacatcg gtgatgaacg cagcaaaacg
    13861 ctggtgcttg aaaccgatca tgtcagccgg agacaggtcg tgcgccacga ggatgcaatc
    13921 ggcaaggttg ctttcggttg acggcaggtt atccggatga ccgagcagca ccttgaggac
    13981 gcgctcaacc acctgcacca cgtcagcctt gcgttctcgc aaataggcgt catcgatggc
    14041 atcaaactgt gccaacaggt gttccatctg ttgcgtcagc gcccattcgg cattgcactg
    14101 ctggctgcgg atcagctgtt tcggcacctc tgacagcatc gaatcgttga ggatcatctg
    14161 atgcatgtcg agaaatgccg gcaactcggc aggcgcgttg gacggaatct ggccgcgcaa
    14221 ctgttccatt tcattgcgca ccgcagcgat tgcatcgtcg aaacgtgcaa tctccttgtc
    14281 gagaaactgg ctcgacagca catagtgcgc cacttcgata cgcgtgtgcg aagccagccg
    14341 ggcaacgccg atggcgattc cgggcgagac gccgacacca tgcagcgtaa agctcattca
    14401 ctttccccga agcggtcgga aatcagtgcc agcaaggctt gcattgcctg cgtttcatcg
    14461 gggccgtcca cttcaacatg aatcaccgac cccttggccg ctgccagcat catcacgccc
    14521 atgatgctct tggcattgac gcgacgctgg ttgcgcgtga tccacacatc gctgctgaac
    14581 tggctggcca gctgtgtcaa ctttgccgag gcacgggcgt gcaggccgag ccggttgatg
    14641 atttcaacat cctgctgttg catcgcataa ctccggattg atatacaaca cgccttcacg
    14701 tccccccgag accgccttgt ccaccacttc tgccagcggc agggtgcggt aggtgatggc
    14761 gcgcatgagc atcggcaggt tgatgcccgc gatgccctcc acccggcccg gcgccagcaa
    14821 ccggcacatg gtgttgcacg gcgtggcgcc atagatgtcc gacaacagca gaacgccgtt
    14881 gccctggtcg agccgggcga tccactcgcg tccgcattcc agcatggtca actgggtttg
    14941 tgctgtcacg tcgagtgcag ccattttttc ggggcgccgc cccagtacat gcgtagcgca
    15001 ctggatcagg ctttcgccca aggtgccatg cgccacgatc aatattccga tcatctgccc
    15061 atcatccctg actggtattc cattgcacac gtcgacgcat ggccggcgtc agatagacct
    15121 tgccttgcgc gtcaatcagc atggcctgct gcacgcccat tcgtgccgcc gctgcaagcc
    15181 atcctggcac cccggcaatg aacaacggtt tgctgtccac atccgacaat accccggcct
    15241 gtggccccgg tggaatcagc actgtcaccg aacgcacccc ttgcgccggc caaccgtcgc
    15301 gcggatcgat gatatgacaa taccgtttcc cacccacctc gaaaaagcgc tgataatcac
    15361 cggaggtacc gatggcctcg ccatcgtgca gcaccacact ggcgatcgcc tccggcttgc
    15421 gcgggtcctg aatgcccact cgccacggcc ggtcaccatg ccgtcccagt accatgatgt
    15481 tgccaccgat gttgaccagc gcattgcgga tgccacgccg ccgcagctcc gccgccgcca
    15541 catccagcgc ataacctttg gcataaccgc cgaggtcgac gcgtacccac ggattggtac
    15601 tgtatgcctg tccattacgg atgacaatgt cgctcatgcg cggttgccgc gcgaccagcg
    15661 cgcgaatggc agccggatcc ggcaggcgcg cctcgaaatg atcggattcg aacccccata
    15721 gccggatcag gttgccgatc gccgggttga acacctcacc cgaacgctgt gaatcagcgg
    15781 tggcgtctcg caggatggcc gccaggtcag gcgccaccgg caccgtacgc ccggcggcga
    15841 acgccgcgtt gagctggtcg agttcgcctg gctcccaggc atgcaaccga tggtgcaggc
    15901 gatcaaagat ggccagcact gcagtcgccg cagcgtgcgc ccgggcttgc ggcgcaccat
    15961 aaatcaccac ttcgacgcgc gtgccgaaca catagctttc ctggctgtac aacggctcgc
    16021 gctgcgtaca gcccgtcagc aaccacagac agcccagcag ccacagcacc ctgatcaggc
    16081 acatcgacgt tccagtgcat cgaccaccat cgcggcggca tcgcaaccgc tctgggcaaa
    16141 tatttccacc aggccggtcg ggctggtgac gttgatttcc gtcaggtaat cgccgatgac
    16201 atccagcccc acgaataaca aacctttctc ccgcaatgtg gggccgacct gggcgcaaat
    16261 ccagcggtcg cgttcgctga gtggcacacc ggtaccggtg ccgccggcgg cgaggttgcc
    16321 gcgactctcg ccctcggccg g
//
LOCUS       DAIANL010000128         3789 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2408191, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000128 DAIANL010000000
VERSION     DAIANL010000128.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2408191"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          664 a         1027 c         1323 g          775 t
ORIGIN      
        1 cgactgtatg tcagcggtgt cggtgttggt catcgactgg accacgatcg gggccccacc
       61 gccgacggcg acgtttccga tcatgacctt gcgggtaggg cgacggggaa tctggatgtc
      121 gaacatggca aaaggaatca gtgcagggtg aaccgggcaa cttttcccgg ggtgtaaggg
      181 gcgaggtcga tcggttggcc gtcgctggtc agtcggaccg aagcggaatt gccaatgatc
      241 agcgcaacgg gcggggcaac gtcgaacgat gcttgtgatc ctgccgggta cagccgcgaa
      301 atgacacggt gcccctggcc gtcatggatg ttgagccagg cgtcgccgag gaactgcagt
      361 tgcagatggc gggcgttggt cgcggcagcg ggtggcgacg ctgctgcgcc tgtgacgggt
      421 gcaatgtcct tcgccggaac gttctgcgct ggaatatcct tcaccggaat gtcatgcgcc
      481 gggacctgct gcgcaggtag cgcggatgcg tcagcccgcg tcgcgaggct ggccggtgct
      541 gccagaggcc cggtggtgcg ggtgggtgtg gccggaggtg tggcattccc ggtcgggact
      601 accgttacgg cggcgccaga ggatgccggt gcgggtatgc tggcgggcgt aggcaccacc
      661 gttcgggagg tggatgccgg gtggtgctcc gtggccggtg tcgtcggcat gtcgctggac
      721 agccagcgat aggccagcca gggcaggatc agcaccagca gcaggacgat cagcaccagg
      781 aatggccagt agccggggag attgcgcttg agcggaatat gctcatcgtg aatgttgagc
      841 gtgtcgcggt cttgagcgcc ggttcggggc aggtctgcca ccagggcatc ggcatcgagt
      901 ccgacgaggc gggcgtagct gcgcacgaaa ccacggctga atacgtggcc gatctcgctg
      961 aaacggtcgt tttcaagcgc ttcgatctgc ctaggtgtga gcttgatgcg ctcggacacg
     1021 tccccgacgg acaggccgct ggcctgacgg gcgtgggcga gtcgctgacc gacgctggca
     1081 ggaatggacg ggatgccggt gtcgtccatc agtcaaattt cccttgcagc aacaactggg
     1141 tttcgggagc atcagggaac cggttgcgca acagcaggct gtcgctggct tcggtgtcat
     1201 ggtcgccgct cttgtgggca atgcgcacgc cgagccagag caattttgcg ctgggtgcgc
     1261 tgttggcaag cagtctggca atcggccggc tggcctcctg gtagcgttgt tgctggtaat
     1321 acaggttggc gagttcgtaa ttggcttgcg ggtcgttcgg gtcgtgctcg agtgccttgc
     1381 cgaaataggt ttctgcctgg agcggtttgc ccattttcat catgcacagg ccggcattgg
     1441 tcagtgcgct ttgtggcttg gcgtacagcg gattggccag ggcagtggtg aactgttgca
     1501 tggcgtcgct gtattggcca cggttgtaga ggaaccagcc gtaattgttg tggatgtcgg
     1561 ggttgcctgg cgcaagatcc agtgcatgca ggaaattgtc ttcagcgggc ttgttttcgc
     1621 caagcgccat gtagaccagg ccgagcacgt cgtaggccgg ggcgtatctg tcgttggcct
     1681 ggagtgcggt gtgcagttca tcgagggcga tggcgtactg gccgcggctg aaatagccgg
     1741 cgccgagttc ggtgtggatg cgcgcgcgca gttcctgctg atcggccgac gagccggggg
     1801 tactggacgc agatgaaccg ataccgagat cgttgttcgc ggccccggcg gagacggcaa
     1861 ggctacagca aaagccaaac agaacaacgg caagggactt gcggcaattc atggcgatac
     1921 ctcgattctg tgcacggttc gacgggtttt gtcctgaacg cgcccggcga gttgtccgca
     1981 ggcggcatcg atatcgtcgc cacgtgtctt gcggacggtg acgacatggc ctgcctgcat
     2041 gagcaagtca cggaaacgct ggatggcctg gctgtccggt cgccggtagc cggtgcccgg
     2101 aaacgggttg aacggaatca ggttgaattt gcacggcacg tcacgcacca gcgcgatcag
     2161 ttcgcgggcg tgggctggct ggtcattgat gcctgccagc agaatgtatt cgaaggtgat
     2221 gaagtcgcgt ggggcgcgct ccagatagcg ctggcatgcc gacatgagtt ctgccagcgg
     2281 gtatttgcgg ttgatcggta ccagttcgtc ccgcagcgga tcgttgggcg catgcagcga
     2341 aaccgcaagc gctaccggac agtcttcgcg cagacggtcc atggcgggga ccagtcccga
     2401 ggtgctgaca gtgacgcgcc gccgcgacag gccgtaggca tgatcgtcga gcatcaggtg
     2461 cagcgcggcc aggacggcat cataattggc cagcggttcg cccatgccca tcagcacgac
     2521 attggtgatc ggacgttcgc atggcgcagc ggaggctgtc gtgctgcccg caacccgatc
     2581 gccgtcatgg tgctgcgcaa gcgcacggtt ggccaaccac agctggccga tgatttccgc
     2641 ggtgccgagg ttgcggttga acccttgctg tccggtagag cagaaagtgc aggccagcgc
     2701 gcagcccacc tgcgaagaga tgcacagtgt gccgcgggtc tcttccggaa tgaacacggt
     2761 ttcgatgccg ttggcggcac cgacgtcgag cagccatttg cacgtgccgt cgcttgcgac
     2821 ctgttccgta aggatttgtg gtacggttac ggtggcctcg cgagcgagtt tttcccgcag
     2881 tgtccgcgcc aggtcgctca tctggctgaa atcggccacc cctgcctgat ggatccagcg
     2941 catgacctga cgggcgcgga acggcttttc gcccagcccg gcgaaatagc cggtcaggcc
     3001 ggcaaggtcg aaatcaagca ggttctgcgc gggcatatca gcgggacgag ttcaatcctg
     3061 gcgtgggtag aggttgagtg cagggaagaa ataggcaatt tccactgcgg cattttcagc
     3121 cgaatcggaa ccatgcacgg cattggcatc gatgctctcg gcaaagtcgg cacggatggt
     3181 gccgggagcg gcctttttcg ggtcggtggc gcccatcagg tcgcggttgg cgaggacggc
     3241 gttttcgccc tgcagtacct ggaccatcac cgggccggat accatgaagt cgaccaggtc
     3301 cttgaagaac gggcgttcgc ggtgcactgc ataaaagcct tcggcttcgt tgcgcgacag
     3361 gtggatcatg cgtgcggcaa tgatccgcag cccggcgtct tcgaagcgtt ggtaaatctt
     3421 gccgatgaca ttcttggcga cagcatcggg cttgatgatg gacagtgtgc gttctacagc
     3481 catgtatgac tccgtttgtg tggttccgtt aatatttggc cggaacacgt tagcatgaat
     3541 cgataataaa ataaatcttg cgagggcggg tgccgtgccc ggtctctgtc agccctgacg
     3601 gttacgtttg cgctcatttt cggtcaggaa gcgcttgcgc agccggatcg attgcggagt
     3661 gatttccacc agttcgtcgt cgtcgatgaa ttccaccgcg gattccagcg tcagcttgat
     3721 cggcgtggtc aggcgcaccg cctcgtcggt gcccgaggcg cgcacgttgg tgagctgctt
     3781 gcccttgat
//
LOCUS       DAIANL010000129        32251 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_168647, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000129 DAIANL010000000
VERSION     DAIANL010000129.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 32251)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32251)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..32251
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168647"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5858 a         9138 c        10511 g         6744 t
ORIGIN      
        1 gtggtcttgc cgttggtgcc tgctacggcg accagcggga tgcgcccggt gcgtccgggt
       61 gggaacagca tgtcgatgat ggcgacgccg acgtcacggg gcttgccgaa cgacggcgcc
      121 aggtgcatgc gcaggccggg cgcggcattg acttcgacga taccgccgcc ttgcatttcc
      181 aggggaaggt gtacgctgtt gcacaggatg tcgacgccgc agatgtcgag tccgaccatg
      241 cgcgcggcat cgacagcccg tgcagccagc gccggatgga catcatccgt gacatcggtg
      301 gctgagccgc ccgtgctcag gttagcgttg ttgcgcagca gcaccagcgc acctttggcg
      361 ggcacgcttt ccggggtgta gccctgtttg gacaaggtgg ccagcgcaat ttcgtcgagg
      421 cggatcttgc tcagtgacgt ggcgtggcct tcgctgcgcc ggctgtcacg gttcacttct
      481 tccaccagcc cggcgatgct ggtttcgccg tcaccatata cgtgtggcgg atcgcgccgc
      541 gcggcggcaa ccagtctgtc accgaccacc agcagtcgga aatcgtggcc ggtgatataa
      601 cgttcgacca gaaccggcgt gccgacccgg gctgcggcgt gccatgcggc catgatctgc
      661 tcgcgtgatt cgaggttgac ggcgacgccg cgaccctggt tgccatattg cggcttgacc
      721 gctactgcgc tgcctatttc ctgcgcggcg cgccaggcat cttcggcatc atgcacgggt
      781 cggccgacgg ggactggaac gccgatagcg tgcagcagac gcttggtcag ttccttgtcc
      841 tgggcgatgg cttcggcaac cgcgctggtc tggtcggtct cggcggccag aatgcggcgg
      901 ctgtgactgc cccagccaaa ttgcaccatg ctgccttcgg tgaggcgccg ccaggggatg
      961 ccacgacgta tggctgcatc gacgatcgca cccgtacttg ggccaagccg gatgtcttcg
     1021 tcaatcgtgc gcagttcggc cagcgctgct acggcgtcga aggccgtgtc ggctgctgct
     1081 gcgtggcaca gttcgagcgc cagttcgagt gcacggcggc cgacagcttc ttcactgtag
     1141 gcgaacacca cctgatagac gccgctgtcg agcgtggccg tggtgcggct gaaggtcacc
     1201 ggacaaccgg caaatgcctg cagcgaaagc gcggccactt caagggcatg cgccatgggg
     1261 atatggtgac tgccgccttg cggttgcagt gaaggcatct gcggaaagcg cgcgcgcagc
     1321 gccggctcga aaccgggaag tcgcgtcagg tcggtttctg tctcgtcgca atgaatgacg
     1381 gtttcgatgg atgtgtgccg gctccacagg ttggggccgc gcaacgcacg gatgcgggaa
     1441 acatgcatgt cggattatcc ttggttgtcg ttccaggccg tcagccccgc cagaatcagt
     1501 tccggttcga cgccaagcgc ccacacggcg gcgatgccag ccagcgcctg ggtcaggctc
     1561 agggtatgcg gcagcagggc agtggccagc aaggcggttg actgtccggc attgtccagc
     1621 atgacggcag cgtcctgtac gtagacggcg cgtccggcat tgtgcaggtg ctggtgtagg
     1681 gcttcctgat cgtggcgggc ggaaaacagt atcgtctcgc cgtcgcatag cgacatcagc
     1741 tgcggggttt ccgggctggc ggcatcgagc acggccgcgc cgtgcggcaa taccacatcg
     1801 atcagtgtgc ggtagatcat gaaacgccgc tccggcgtgg tcatggcgta ggcagagaaa
     1861 tcttcctgcg ggtgcaggtc ggtcagtacc gcaacctggc agcggtcgaa tgcaagtcca
     1921 tgctgcagca ggtcgtgaaa gccggtttcg cctacggcgc agctgactcc ggggttgagc
     1981 agcagtttat gcatcccctg ccagcccgag gcgtcgccga tttcgatctg gcgctggtcg
     2041 aggaacaatc cttgccgcga tgccaggccg acctgcttgc gaccgatctg tagcagaccg
     2101 gcgatgagcc gggcagtacc gctgcggccg ctgccggcgg tggcgacgat ggggatgcga
     2161 cccgtgcggc cctgaggaaa cagatgttcg acaatggcgg cgccgaccgg tcgtggctga
     2221 ccttcagcgg gtttcaggtg catcaacagg ccgggcccgg cattgacttc cacgatggca
     2281 ccgcgctgct cgtgcagcgg tcgggaaatg tcgtctgcga cgagatcgat gccggcaatg
     2341 tccagtccga ccacctgtgc tgccagcacc accagctcgg ccgtatcggg gtgcacctgg
     2401 tcggtgacgt cgatggcgac attgccgatg cgttgcacga tgaccttgcg ccccgctggc
     2461 gggatgtcgt cggcgatgaa tccctggcgt tccagttcga tccggcaggc agaatcgacg
     2521 tgaacctggt tgagcgggtg ggcttcggta tcgcctcggc gcggatcggt attgatctgc
     2581 gatccgatca gctgacgtat cgtgctgtgg ccgtcacccg tgacagtggc gatctcgccg
     2641 cgtgccgcag cgaccatgcg atccccaacc accagcaggc ggtgttcctg accgctgatg
     2701 tgtcgctcca cgagcacgtc gctgtcttct tccgcggcga ctgcccacgc ggtttcgatt
     2761 tccttgcgcg tcatgagatt aacgaatacg gcgcgaccat gattgccact caacggcttg
     2821 atcaccactg gcaagccgat atcctgggcc agttcccaga cggtggcagt atcgtgcgcc
     2881 agttctcctt ccggcacagg aacaccgcac tgccgcagca attccttggt cagtgccttg
     2941 tcgcgcgaga tggtctcggc aatggcgctg gtgcgatcgg tttcggcggt ccagatgcgg
     3001 cgctgcgcct ggccatggcc cagttgcacc agattgccgc gcgtgaggcg gatggccggg
     3061 atatcgcggt cgtcagctgc atctacgatg ctgttcgtgc tcgggccgag caggaatgac
     3121 tcggccaggt cagtcagctc ttcaaccgca gcagccacat cgaacggggc agcacgcatt
     3181 gcggccagca ccagttcgcg gccgagatcg agtgcgcggc gggtgacatc ttcgtggaaa
     3241 gcgcgcacca cgaccttgta cacgccgcgt tcatccatct cgcgcgcgcg accgaagccg
     3301 gcgggcatgc ctgccatggc ctgcagttca agcgtgacgt gttcgagaat atgtgccggc
     3361 catgtgcctt cgtgcaggcg ttgcaggaag ccgccgcgta cgccggggct gcaatggtgc
     3421 tcgatcagtc ccggcagcca ctgggcaagg cgttcggcgt agcccggaat gcggtgcgaa
     3481 ggccagtctt ccagcacacc gatatcgacc catgcctcaa ggaccggccg ataggtccag
     3541 atgttgggac cttgcaggga aatgatacgc agaaagcgaa tatccgcgtc tgcgggaaag
     3601 tcagccggtt gattcggaat gcaggaatgt tgcatggagt atgtcagtgt cgggtcagtt
     3661 tatgctgtac tcaatcataa cgcaccagta gaattcaggt ttacctgatt gcctgatttt
     3721 attgatcgtg actgttgcaa ccgatcccat gtcctgtttg ccgatcgcct ggcgtggtcg
     3781 tctggcattc ggtttttacc ccggtgaagt tgtgcttgcc gtgctggaaa cggatctgga
     3841 cgaacacctg tatttcagat ccggtttgct ggtcttgacc gggcagcgtt tgctgggtgt
     3901 cggcgccgat ggccaactgc tgcatgacta tcccgtgact gcaacactgc gtctgacacg
     3961 ccgcgaccat gcgggcgtgg gtgtgctggg tctggatcgc gatggcgtac ggctggcgac
     4021 ctggcggtat tcgctggcgc gtgcagcggc ggcgggtcgt gttgaggcga atttccacgc
     4081 ggtgcaggcg ggcacggatg caagcaccct gattgccgaa gcgctctgcc cgcaatgcgc
     4141 gcagccgctg ccggaggccg atgcaatgtg tccgacctgt gcgccggtgt ccgaggacgc
     4201 cgcgcagtca ggctgggcat tgctgcgtct gtggcgtttc gctcgcccgt acgggctgtc
     4261 gctgctgggc gggttcctgc tcacgctcgg ttcgactgcg gccacgttgg tgccgcccta
     4321 tctgaccatg ccgctgatgg acaaggtgct gatcccttac cagaccggtc atgcgatcca
     4381 tatgtccatg gtggcggggt atctggtggc cttgttgctg gccgcgctgg tggcctgggg
     4441 gcttggctgg ggacgtacct ggtggctggc gcgcgtgagc gagcggattt gcatggatct
     4501 gcgactggcg acgtatcgtc atttgcaaag cctgccgctg gcttactttg gcggcaagcg
     4561 caccggtgac ctgatttcgc gtatcggcag cgagtcggat cgcatttcgg tgttcctgtc
     4621 gctgcatttg ctcgatttcg tcatcgatat gctggtgatg ctgatgacgg ctgccatcct
     4681 gctgtcgatc aatccctggc tcgcactggt gactttgctg ccgatcccgg tgatcgcctg
     4741 gatgatccat cgcgtgcgcg acaggctgcg cttcggtttc gagaaggctg accgggtctg
     4801 gggtgagatc agcaatgtgc tcgccgacac cataccgggg gtgcgcgtgg tcaaggcatt
     4861 tgcccaggag cagcgcgaaa tcgaccgttt ccgggaagcc aatcagcgca atctgcagat
     4921 caatgatcag gtcaatgcca tctggtcgct gtttgcaccc agcgtgacgc tcctgacgga
     4981 gatcggcttg ctggtggtgt gggcattcgg catctggcag gtcgggcatg gtcagatcac
     5041 ggtgggcata ctgaccgcat ttctcaccta catcagccgt ttttacaccc ggcttgattc
     5101 gatgagtcgt atcgtgtcgg tgacgcagaa ggcggcagcg ggagccaaac gcatcttcga
     5161 gattcttgat tatcagtcga gcgtgcccga accggccaat ccggtgcacc cgaccgcgct
     5221 gcgtggacgg attgcgttgc gccacatcgg gtttcgttat ggcaaccgca gcgtgattca
     5281 cggcatggat ctgaccatcg agccgggtga gatgatcggc ctggtcgggc acagcggttc
     5341 gggcaagagt accctggtga atctgatctg ccggttccac gacgtgagcg aaggagcgat
     5401 cctggtcgat ggcgaggaca tccgcaattt ccctgtcagc gaataccgcc gccacatcgg
     5461 gctggtgctg caggaaccat tcctgttttt tggcaccatc gccgaaaata ttgcgtatgg
     5521 caaaccccac gccacgcacg aagaaatcgt aaccgccgca cgcgcggcgc atgcgcacga
     5581 attcatcctg cgcctgccgc aaggttatga ctcgctggtg ggtgagcgcg ggcaggcgct
     5641 ttcgggtggc gagcgccagc gtatttcgat tgcgcgtgcc ttgctgatcg atccacgcat
     5701 tctgatcctc gatgaagcca cggcctcggt ggataccgaa accgaacgcg agatccaggc
     5761 tgcgctcgat aacctggtgc gcggacgcac gaccatcgcc gtggcgcacc gtctgagtac
     5821 gctacgacat gccgaccggc tggtggtgct ggagcgcggt cgcattgtcg aggtcggccg
     5881 gcatgaaacg ctgctggcca tgaatggcgc ctatgcccaa ctctacgaag cccaggcacg
     5941 ccaggtcgat gatgaacgcg aaggagagtt gcttgccgat ccagtctgat gttgccggaa
     6001 gactgacgtt catcgacgct cgcggagagc ggcacgatgc gttgctgcca gtgcgggcat
     6061 tccccctgag tgcgccgcag ggcagtgtca gtctggtgga tgatcgcgga caggagtgcc
     6121 tgtggattcc cgaattgagt gaattgccgc ccgccgagcg tcagtgcgtc gaggcagcgt
     6181 tggccgaacg tgagtttgtg ccgaaaattc tcaccatcga ggccatcagc ggctattctc
     6241 tgcccagcga gtggcgcgtg cgcaccgacc gcggcgagac gacgctgacg ctcaaggcgg
     6301 aggaggatat ccgtcgcctc agtggcgggc gcgtgctggt tgcttcagcc agcggggtgc
     6361 agtttctcat tccggacgtg agccggctcg accggatgag ccggcgcaat ctggatcact
     6421 gcatgtaaga aggcgcgcac gtttagccgg cagggatgtg tgccagcgcc atcagcagca
     6481 gaatcagtgc ccagatggcg agcgtgcgcc agatcaggct gttgacgccc gccaggtcat
     6541 gcagcgtggc gttttcgcca agaccgaatt ccggcaatgt cgctgtggcg ggcaggcgaa
     6601 tgccgagcgc cccggtaccg ctggccagca cctcgccaag gttgctgtcc gtgttgtgtg
     6661 tggcctgact gcgccagcag tacatggcgt cttcgaagtt gcctgcaacc gcgaaactca
     6721 gcgctaacgc ctgcgccggc agccagtcga tgcgccatac ggccgagtgg gcgaagtggg
     6781 cgaaggcacc gtgggtgtcc ggatcccagt gttcggcgat attgtgactg aggcggtata
     6841 agacggcgcc gaccggcccg aggggagtga gcagtgcgaa ccagaaaatg acgccgaaca
     6901 ggtgccgcag tgcatccgcc agccggtttt cgatggcgat gcgcagggtg tcgccttcgc
     6961 gctcatccat gttgccgtcg caccactgca atgcttcctg tcgtgccact tcgggctggc
     7021 cgcgcagcag tgcggtcagg atattccggg tgacccggtt ctggcagcgc agttgcaggc
     7081 tggcatagag caccgcgata ttccacagcc aggccagcca gcccgaaagc gcgagcaggc
     7141 cgaaatgcac tgcgctcgtg acgagcagcg ccggcagcat ggcggcgaac catgcgagcc
     7201 tgccgtggcg gtgctcgcct gcattcagct ggtgtgcgag aaactgcaac ggcaacatca
     7261 ggcgttcgag ccacaaacgc ggcagcggac ggtagtgttc cagcagcagt gcgaccagca
     7321 ggatactcag ggtcaagacg atctccctga aaagagcggg atgaggtggc ggaccgggtt
     7381 catggccgcc gtctgtcagt ccgccatatc gacggtcagt gtgcccatct ggctgacgga
     7441 atggcccgcg gtatagattt cgaccgtggg caggcggatg tcgcgaccct gcggcctgag
     7501 ataatcgaac agtgcctggt aggccttgct cggtgcaagt tgcatgctcg cctgtacgct
     7561 ggcgcgcaac acgttgcgtg ccgggatatc atcgagcctg agtggctcgg ggactgcggt
     7621 accgggtggg atctcgtaac cgacgcgagc atgctgctct gtcttgcggg tattgcgtgg
     7681 gtcggtgtag agcaccacaa ccggactgcc gggctggatg cgcgccgcca cgagcatgcc
     7741 gcgcaccttt tcctgagcgt cggcgatggc gccgagatct ccgaaaaagt cggtataggc
     7801 gtaatgaacc ggttcgctgc gggtttcgcg gatgtcggta cggttgaatg cgccccacca
     7861 gaacaggacc acgaggatcg gcacgagaaa cgcgaaaacg aaacctaccc agtttttctt
     7921 gatcaaaatg gctccggagc agaacgtcag gcgaggcaaa gataacacaa ccgccgcggc
     7981 ttgtcgcggc gcgcaccccg caggcggctc atgtttgccg gtggcagcct gcggccggac
     8041 atgatacatt cgcgctgaac cggggccggc tcgggcattc ggacgattgc aattcaggag
     8101 actacatgaa acacacccaa tcttatggtg ccctgctggt gtcgctgcat tggctggtag
     8161 ctgctttgat cgttctggta ttcgcactgg gatggtggat gggcgacatg cccaagggac
     8221 cggacaaggt gcagaccatg ggttggcatc agacggtcgg gatcagcatt ttcgtgctcg
     8281 ccatcgtgcg cctggcctgg cgacgcctgc acggcgtgcc tgaaatgccg ctgaccacgc
     8341 cgatagaaaa actggcggtg ctggtgcaga ttgcgctcta cctgctgatg ctggccattc
     8401 ccgtgtttgg ctatctgacc agttcgtttg gcggccatgc tgtcgtggtg ttcggttcgc
     8461 tgaccattcc cggaattacc gccgtcgatc atgacgtgca cgaaacgatg ggagacctgc
     8521 accaggcgct ggcctacggg ctgctcgggc tggtggcatt gcacgtggcc ggcgcgctca
     8581 agcatcacct gatcctgcgc gacgatgtcc ttgcgcgcat ggcaccctgg gttcgcaaac
     8641 gctgacctca tgttctccgg gggggctgca gcctccccca tcgtcttcgc tgctgctata
     8701 atggcccgtt tatggattgc gggtgaacgt catggaagcg gaacgtctga atcagatcga
     8761 tggtatgttg cgggatctcg gcgatcgtgc cgcggcttta cggaggtatc tttgacttcg
     8821 atggccaatc gcaacgtctc gaagaagtca atgcgctggt agaagacccg gcaatctgga
     8881 atgaccccaa gcgcgcccaa tcgcttggac gcgagaaaaa atcccttgaa ggcgtggtgc
     8941 tgaccttgcg gcggatcgac gcaggattgc gcgatgcgcg cgacctgttc gacatggcac
     9001 gcgaggaaaa cgacgacgac acgctggaag ccgtggccgg ggacgtggcg cagatcggca
     9061 atgatgtggc gggcatggaa ttcaaacgca tgttctccaa tcccatggat ccgaacaact
     9121 gcttcgttga catccaggca ggcgccggag gtaccgaagc ctgcgactgg gcatcgatgc
     9181 tgctgcggca gtacctgcgc tattgcgagc gcaaaggatt ccgcgccgaa ttgctggaag
     9241 aatcggccgg cgacgtggcc ggggtgaaat cggccaccat tcgtgtggat ggcgaatatg
     9301 cctacggtca tctgcgtacc gaaaccggcg tccatcgtct ggtgcgcaaa tcgccgtttg
     9361 attcgtctgg cgggcggcat acctcatttg ccagcgtgtt cgtctatccg gaagtggacg
     9421 attcgattga aattgatatc aatcccgccg agctgcgcat cgatacctac cgtgccagtg
     9481 gggccggtgg ccagcatatc aacaagacgg attcggcggt gcgtattacg cacgtgccaa
     9541 gtggcatcgt ggtgcagtgt cagaacgatc gttcgcagca caagaaccgt gccgaggcca
     9601 tggccatgct caagtcacgg ttgtatgaag ccgaactgcg caagcgtcag gccgagcagg
     9661 acaaactcga agccggcaag tccgacgtgg gctgggggca ccagatccgt tcctacgtcc
     9721 tcgaccagtc gcgcatcaag gatttgcgca ccaatgtcga aatctccaat acgcaaaagg
     9781 tactggacgg cgacctcgat gaattcatcg aagccagcct gaaacaggga gtgtgagcat
     9841 gaacgaacag gaactcgaac cgggcagtgc ggccgatgag aaccagatca tcggcgaacg
     9901 gcgtgccaag ctggctgaat tgcgattggc cggagtggcc tttcccaatg atttcgatcg
     9961 gcgcgatctg gcgggcgatc tgcacgcgcg tcatggcgaa tctgccaacg aggccttcga
    10021 aactgcgccg gtaacggtgc agattgccgg tcgcatggta ctcaaacgcg tgatgggcaa
    10081 ggccagtttc gccacgctgc aggacagttc gggccgcatg cagatttacg tcaccaatga
    10141 tcaggccggc gaatcggcgc accaggcatt caagcgctgg gacctgggcg atatcgtcgg
    10201 cgtcaccggc gtgctgtttc gtacccgtac cggggaactc acggtgcagg cacaggcagt
    10261 gcggttgctg gtcaaggcat tgcggccatt gccggaaaag tttcacggcc tcaccgacca
    10321 ggaacagaaa taccgtcaac gttacgtcga cctgatgacc agcgatgcgt cacgtcaggt
    10381 gtttcttgcc cgtttccgca tcgtgcaggc gattcgccag ttcatgaccg ggcatggatt
    10441 cctcgaggtg gaaacgccga tgatgcatcc gattccgggc ggagcggcgg ccaaaccttt
    10501 cgtcacccat cacaatgcgc tggacatgtc actgtacctg cgcatcgcgc cagaactgta
    10561 ccttaagcgg ctcgtggtgg gcggtctgga aaaggtgttc gaaatcaaca ggaatttccg
    10621 caacgaggga ttgtccaccc ggcacaaccc cgaattcacc atgatggaat tctacgaggc
    10681 ttaccgggat taccgctacc tgatggattt taccgagcac ctgctgcgcg acgtggcacg
    10741 gactgcggtt gcgacactgc aagtgccatt ccaggatggc gtgatcgacc tggaacccga
    10801 tttcgaccgg ctgaccatcg tcgaggcgat ccgcaaatat cacccgcaat atgccctgtc
    10861 ccagctggaa gatcgcgcct ggctactcga atggtttgcg gccaaccgcg agaagcaccg
    10921 tcccgatgac gggctgggcg ggttgcagct gagctttttc gaggccacca ccgaagcgtt
    10981 gctggtgcaa cctacgttca ttattgatta tccggctgaa gtttcgccgc tggcgcgtcg
    11041 gtcggatgcc catccggaaa tcaccgagcg cttcgaattg ttcgtcgcgg ggcgtgaaat
    11101 tgccaatggc ttttccgaac tcaacgatcc cgaagaccag gcagagcggt ttctgctgca
    11161 agcgcgtcag aaagaggcgg gagacgaaga ggccatgcac tatgacgccg attacattcg
    11221 tgccctggaa tatggcctgc cgccaactgc gggttgcggt atcggcatcg accgattggt
    11281 catgctgctg actggcagcc agaacatccg cgacgtcatc ctgtttccgc acatgcgcgc
    11341 tgaatgacgc gcagcgctta ttcgcttatc cggcggcgct tttgccgccg gaaatggaat
    11401 ttcagcgcca gaaccatgct cgccagcatc agcgtgacgc tattggccac gatgaccggc
    11461 caggcatgca ggcggatgcc atagcacagc cagagcccga caccggtgat gaaaatcacg
    11521 aacatgctgg tggagatgtc ttccgcatga cgggtacgcc agatgcgtat tacctgagga
    11581 ataaaggaca acgtggtcaa cgtaccggca agcatgccga tcagatcgga ttcgggcaat
    11641 cgggttcacc agaagtaggg ataaggataa ggatacatgc cccagggtgg cgcgaacgga
    11701 tattcccacg ggccgaacat ggggctgccc cagcctggcc atgggccata cgccggattg
    11761 tcgaatcgtg ggtcgtaagg cggagtggcc ggggtgacgc gggccgcagc gacccagggc
    11821 aggttgtcga aactttctgg ctgcagaatg ccgcgtgcgc gcaaccagtc gccggtttcg
    11881 taattgctgc cggaataata accgaaggcg cacacctcga atacctcggg catggtgcgg
    11941 tcggtttcct gtcggctgcg gcgcagcttg aagcaggtgt cttcgtcatg ccgggtttgc
    12001 gctacaatct gtccggtcca gtcgatggat ttcccggcag ggtcgaaacc ggcccatgcc
    12061 ggcagggcaa gacaactggc aaacagtgcg taccatactc gcatgatcgg gtcccccgaa
    12121 tgaagggtcg tgatagcagt gtactatagg gtaacaggac aatgagtgaa agcataaccc
    12181 acattacctt gcatccgcga gaagaaaaat ctgcagtggc agcgtactgg cagacttatg
    12241 ccgcccgttt tcccgggctg gaactggaag tcgcggaact cgattgtggt gattacgcgc
    12301 tgggtggcgg tgtgcgcgtc gagcgcaagt cggcgacgga tttcaatttg tcggtcatgg
    12361 accggcggct gtatggcgag gtggcccggc tgaaagcggt ggccgaccag gtcgtctacg
    12421 tggtggaggg cgacctgttt gccgaacgtt ttcacagcga cccgcaactg acacgcgagg
    12481 cgctggcgtg gatgacggcc ttgcaggggg tggcgctgct gccgagttca ggtgaggcgt
    12541 tcagtgcgga aatgctgttt tccatggcga agctggcgca gcatggtttt ggtcagccac
    12601 cggtgttgcg caatggcaaa ccttttgatc ctcgcggcgc tcaactgtat gtggccgaag
    12661 gcttgccggg aatttcaccc gtggtggccc gccgcctgct tgaacatttt ggcacgctga
    12721 ctgcgctgtg tacggcaccg gtcgaggcgc tggcccaggt cgaaggtgtc ccggttcaac
    12781 tggcgcaacg catccgcaag gtgctcgatg ccgaattcag gtccgcctga gcgattgaag
    12841 aaggagtgtc catgaatcag accgcgcagg aaattgccat gttgcgcagg gagcttgaaa
    12901 tgctcatgcg cgagcgtgaa gcgctgttgc gcgtcggtgg agcggctgcc ctgttcgttg
    12961 ctgagctcga cagtctgcag ttgccggaga agacctggaa tgttgccgag tttctggcgg
    13021 aatcgctcaa catgttgaac gaagatacgt tgcaggaagc gctcgagacg gtagggtctg
    13081 ccgtacagcg gtccgaaatg aacgacgctg cgcagtctga agcaggaact tcctgacgac
    13141 gggtgctaat ccgtgtcgag gcacaggtcc agcgccgtct gccagtgggg catgacgata
    13201 ccgaacgcgg catgcagctt gctgttatcg agaaccgagt tggctggccg actggccggg
    13261 gtggggtagt cgctggccgg gatcgggtgc aaggtgacct gtcggccttc catgagcggg
    13321 gtgcggatga gaatggcttc ggcaaaatcg tgccacgaag cgctgccgcc gcaggtcagg
    13381 tggtagacgc ccgacagttc gggctggacg aaagggcggc gcgcaaggag ttgcgctgtg
    13441 gcttccgcga tgctgcgcga ccaggtcggg gcaccgatct ggtcggcgac gatgcgcagg
    13501 ttgtcgcgtt cgcggaatag ccgctgcatg gttagcagga aattcccgcc ccgtaggcca
    13561 tacacccaac tggtacgcag gatcaggtgg gcgatgccct gttcgcggat ggcctgttcg
    13621 ccttcgagtt tggttgcgcc atagacgccg agcgggtgcg tgatgtcggt ttcaagccac
    13681 ggcgtttttt tgctgccgtc gaagacgtag tcggtggaaa agtgcaccag cggtacgcca
    13741 aggcgggcgg cctcttcggc gagaatggct ggtgcgatgg cattgatggc ccgtgccaga
    13801 gcgggttccg attctgcgcg gtcgaccgcg gtgtgtgcag ccgggttgac gatcagttcc
    13861 ggtttgagcg tgcgcacgaa atcacgcaga ttatgcatgt cgctcaaatc gagcgcatcg
    13921 cgtcgcggcg cgacgacttc tcccaacgtg gccagcgtgc gttgcaattc ccagccgacc
    13981 tggccgctgg agccggtgag caggattttc atgcaaaggt ctcgcactga gccagcggca
    14041 ggccggcggc atctttggcc gcaatcgtcg gttcgccgtc cagcggccag gagattgcga
    14101 gcgcggggtc gttccacagc agacagcgct cgtgcggcgg cgcccagtaa tcggtggtct
    14161 tgtagcggaa ctcggccacg tcggaggtga ctacgaaacc atgggcgaag ccgggtggga
    14221 tccaggccat gcgccggttt tctgccgaca gttccatacc cacccactga ccgaatgttg
    14281 gcgaggaacg acgcagatcg accgccacat cgtagacgct gccgacaatg gcgcgcacca
    14341 gtttgccctg ggtgtgctcg atctggtagt gcaggccgcg cagcacgccg cgacgtgaac
    14401 gcgaatggtt gtcctgcacg aattcctgcg gaattcccag ttcgctgaag gtgcggcggt
    14461 tgtagctttc atagaagaaa ccgcgctcgt caccgaatac ccggggttcg atgagcacga
    14521 cgtcggcaat ggcggtgggc aggatattca tgttcagaac aattggtcgt gcagcatgtt
    14581 gagcaggtag cggccatagc cgtttttctt gagtggctgg gccagccgtt cgatctgttc
    14641 cgcactgacg tagcccaggc ggtaggcgat ttcttccgga caggctattt tcagtccctg
    14701 acggttttcg atggtctgga tgaaggtgga tgcctccagc agcgactcgt gggtgccggt
    14761 gtcgagccag gcgtgtccgc gtcccatgac ttcgacctgt agctgcccgc ggcgcaggta
    14821 ttcgagattg acgtcggtga tttccagctc gccacgcggc gacggtttga ggttgcgcgc
    14881 gatttcgacc acctgttgat cgtagaaata caacccggtc accgcatagc gcgattttgg
    14941 ccgcgccggt ttttcttcaa ggcttaccgc tttgccctgg gcgttgaact cgaccacgcc
    15001 gtagcgttcc ggatcctgca caggataggc aaataccgta gcgcctatct cgcgtgccga
    15061 ggcggcacgc aaatcctggg caaattcatg cccatagaac aggttgtcgc caaggatcag
    15121 cgtgctgggc tggtcgccga cgaaatccgc gccgatgatg aatgcctggg ccagtccgtc
    15181 cggcgagggt tgcaccgcgt attgcaggtg gatgccccac tgactgccgt caccgagcaa
    15241 ctgctgaaag cgtggcgtgt cgagtggcgt ggatatgatg agtatgtcac ggatacccgc
    15301 cagcatcagg gtgctgagcg ggtagtagat catgggcttg tcatagactg gcagcagctg
    15361 cttggatacg gcctgggtaa tggggtacag gcgggtgccg gaaccgccgg ccaggatgat
    15421 gccgcggcgc ccgctcacgt gcgtgctcca tagtgccgtt cgagccagtg gcggtactcg
    15481 ccactggtca cggcttcgac ccagggctgg ttgtcgaggt accaggccac ggttttgcga
    15541 atgccggttt caaaggtttc ttctggcttc cagcccagtt cacgttcgat cttgcccgcg
    15601 tcgatggcgt agcgccggtc gtggccggcg cggtcgcgca cgtggtggat cagtgcgcgg
    15661 tgcgagcctg tctgcggtcg cgcctgatcg aggatgtcgc acagcgtgtg cacgacttcc
    15721 atgttggttt tttcgttcca gccgccgata ttgtagactt cgccggggcg accggcgttc
    15781 aggacggtgc ggatggcgct gcagtggtcg ctgacgtata gccagtcgcg gatgttgagg
    15841 ccatcgccgt agaccggcag gggcttgccg ctcaatgcgt tgtggatcat gagcggaacg
    15901 agtttttccg ggaactggta tgggccgtaa ttgttcgagc agttggtcgt gagcgtcggc
    15961 aggccgtagg tgtgatgcca ggcgcgaacg aggtggtccg aagcggcttt ggacgcagaa
    16021 taggggctgt tgggcgcgta gggggtgtgt tcggtaaacg gggcatcctg cggcccgagc
    16081 gaaccataga cctcatctgt ggacacgtgc aggaagcgga acccggcctt gtcgtcggtg
    16141 cccagttgtt cccaatactc ccgtgctgcg ccgagcaggg cgaatgtgcc attgacgttg
    16201 gtgcggatga agtcttccgg cccatgaatc gagcggtcca catggctttc cgcggcaaag
    16261 ttgatgatgg cccgcggctg gtgttcctgc agcagatggg caaccagtgc agaatcgccg
    16321 atgtcgccat gcacaaacag gtgacgagga tcgtcggcca gtgaatcgag attgtgcagg
    16381 ttgcctgcgt aggtcagttt gtccaggttg acaacgggtt cgctgcagga tttcagccag
    16441 tccagtacga agtttgcgcc gatgaacccg gcaccgcctg ttacaagaat catgtggatg
    16501 cgctctttct tgcgtgtgtt ggcacaaggg cagcggtgat gcgcggccgg gtccgccatc
    16561 cgtaccggat aggcaggatg acctgcgcgc aacggatgac ctcggttcca gtgttacgaa
    16621 tgcacggcga caggaatcga tccgctatta gcctggtcgc ggtcatttct ccgcaaccgt
    16681 cgcgattgta acagagctgg atgcattgtg tgcaatcaga cgagagcgtt ttcttcctcg
    16741 aactgcagcg cgacctgctc gttttcgtcg atgtcgagcg tgacctttcc gccattggcc
    16801 agccgtccaa acagcagttc gtcagccagt gcgcgccgca gcgtattctg gatcagccgg
    16861 gccataggac gggcacccat cttcggatcg aatccgttct tgcccagcca gttcttgagc
    16921 gcgtcggtga aatgcgcgtc gactttcttt tcgtgcaact ggtcttcgag ctgcatgagg
    16981 aatttgtcga ccaccaggcg gatggtgtcc ggcgacagcg gagcgaacga gaccgtggca
    17041 tcgagccggt tgcgaaattc gggtgagaac atgcgctgga tgtcgcgcat ttcgtcgcca
    17101 gcttccgccg tgctggtaaa gccgatgctg gccttgccca gcgtttccgc cccggcattg
    17161 gtcgtcatga tgaggatgat gttgcggaaa tcggcacgtc ggccattgtt gtcggtcagt
    17221 gtgccgtgat ccatgacctg cagcagcacg ttgaaaatgt cgggatgcgc tttctcgatc
    17281 tcatcgagca acagcaccgc gtacgggttc ttggtgatgg cctcggtcag caggccaccc
    17341 tgttcgaatc cgacataacc cggaggtgcg ccgatcaggc gtgacacggc gtggcgttcc
    17401 atgtattccg acatgtcgaa gcggatcagt tcgatgccca gcgtgtaggc caactgacgc
    17461 gcaacctcgg ttttgccaac cccggtcggg ccggagaaca ggaaggcacc gatcggtttc
    17521 tgcgggttgc ccagaccgct gcgcgccatc ttgatggcag ccgacagcgc ggtgatggcc
    17581 gcatcctggc cgaacaccac ggccttgaga tcgcgttcca ggttgcgcag ctgcagatgg
    17641 tcgtcagtgg agaccgtttt ggacggaatg cgggcgattt tggcgaccac atcctcgatt
    17701 tccttgcgcg taatgacctt cttctgtttc gaacgcggca aaatgcgttg ggctgccccg
    17761 gcttcgtcga tgacgtcgat ggccttgtcc ggcaagtgac ggtcattgat gtagcgtgct
    17821 gccagttctg ccgccgtggt cagcgccgat agcgtgtagc ggatgttgtg gtgtgcctcg
    17881 aagcgcgatt tgagcccgcg caggatggcg acggtttctt ccacgctcgg ctcgtcgaca
    17941 tcgattttct ggaaacggcg cgacagtgca tgatcttttt cgaaaatgcc gcggtactcg
    18001 ttgtaagtgg tcgcgccgat gcacttgagc tgtcctgacg acagtgcggg cttgagcagg
    18061 ttggaggcat cgagcgtgcc gccggacgcg gcgcccgcgc caatcagcgt gtggatttcg
    18121 tcgatgaaca gaatggcctt gctgttggct tgcagttgct tgagcacggc tttgaggcgt
    18181 tgctcgaaat cgccgcgata tttggttccg gccagcagtg cgcccatgtc gagcgagtag
    18241 atggtgctgt cttcgagcac gtccgggacg ttgccttcga cgatgcgtcg cgccagacct
    18301 tcggcaatgg cagtcttgcc gacgccggcc tcgccgacca gcagcgggtt gttcttgcga
    18361 cgccggcaca ggatctggat gacgcgctcg agttcgtgtt cacgcccgat gagcgggtcg
    18421 atcttgccga ccagcgcccg cgaattgaga ttttcggtga agctctccag tgcgcctggc
    18481 atcgtggatt cttcgccagg ttcggcagcc ggttcgctgc gaggcggttc gctctgcggg
    18541 gttttggcga tgccgtggga caggtagttg acgacgtcaa gtcgggtcag tccctgttgc
    18601 gacagatagt agacggcgtg cgaatccttt tcgccgaaca gcgcgaccag gacgttggcg
    18661 cccgtgactt cctttttacc cgaagattgc acgtgcagga tggcgcgctg gatgacgcgc
    18721 tggaagccca gtgtaggctg ggtgtcgacc tcttctgctc cggccagtgt aggcgtgtgc
    18781 ttggcaatga attccgtgag ttgctgacgc agcgattcga tgtccacggt gcaggcgcgc
    18841 agcacttcgg ccgccgatgg gttgtcgagc agcgcctgca gcaggtgttc caccgtaatg
    18901 aattcgtgtc gcttctgtcg cgcatccatg aatgccagat gcagactgac ttcgagttcc
    18961 tgggcaatca ttcaattttc ctccattacg cattgcagcg ggtgctgatg ctgccgcgca
    19021 taaccggtga cctgcgccac tttggttgct gcgatatccg ccgggtagag cccgcagacc
    19081 cctcgcccct cattgtggac ccgcagcatg atctgggtcg cctgttcctg ggttttgccg
    19141 aaaaaatgtt gcaagatgtg taccacgaaa tccatgggcg tgtaatcgtc attgagtaac
    19201 acgaccttga acatcggcgg gggcgccagt ttgcttctgc ctggcgcgag cagtgtgtct
    19261 tctcgatgcc gtgtcgccat ttcttccgtt gttccttccg ttggccggca tgcgttcatg
    19321 gccagcctcg ggggcgggtt gctgcgcatt ttcatgatcc gttacttgtg cattttgaac
    19381 attcgagtga atttttcaag tattccaaaa aaaataaatc ggctgattca tttaacgctt
    19441 gccaaattgc atgggtagga gtaaaaagta gtccggagtt gggtatcaag gttctagcaa
    19501 gtctggtgct gccgttgtgt gatcttgatt ctcaaacaag taatggccgg tatggtctgt
    19561 tttttatcaa ggaagtaaca atggcaactg ggactgtcaa gtggttcaac gattccaagg
    19621 gattcggttt cattaccccg gacgatggca gcgaagatct cttcgcgcat ttctccgcga
    19681 tcaacatggg tggtttcaag accctgaaag aaggccagaa ggtcagcttt gaagtttcgc
    19741 aaggcccgaa gggcaagcag gcttcgaaca ttcagccggg ctgatcgacc ggccccgtcc
    19801 agtagcggcc atcccgtgat ggccgttcgg tagggtagtg gttttccgca gtttttctgt
    19861 gggcaggggg cgcatacctt gagtttgcgc cctttttgtt tgtgcagaca ccccgacgtg
    19921 tgacgcccgg ggctggtcgc cggtacggac tcgcaccggc ggtgcattcc ccgcaacggg
    19981 gcgcgtgcac tacatgtgtg cgatcatttc ctcgccaaac gccgagcagc tgacctgcgt
    20041 ggcgccgtcc atgaggcggg caaaatcgta agtgacgcgg cgtgcggcga ttgcgccctg
    20101 catgccggcg atgatccggt cggccgcatc gtcccacccc aggtgtcgca gcatcatttc
    20161 agccgaaagg atgatggagc cggggttgac gaggtcctga ccggcgtatt tcggggcagt
    20221 gccgtgagtg gcctcgaaca tggcaacgct gtcggacagg ttggcgcccg gcgcgatgcc
    20281 gatgccaccc acttcggcgg caagcgcatc ggaaatgtag tcgccgttga ggttgagtgt
    20341 cgcgatgacg tcgtattcat ccgggcgcag caggatctgc tgcagaaagg cgtcggcgat
    20401 gacgtccttg atgatgatgc cattgggcag gcggcaccat gggccgccat cgatctccac
    20461 cgcgccgaat tcgcgtcggg caagctcgta tccccacttc ttgaagccgc cttcagtgaa
    20521 tttcatgatg ttgcccttgt gcaccagcgt caccgaacgg cgtttgtgat cgatggcata
    20581 ctggatggcg cgacggatca gccgctcgct gccttccacc gatactggct tgatgccgat
    20641 ggcggaactg tcgggaaagc ggatcttgct gactcccatt tcctgctgca gccaggcgac
    20701 cacccgtttt gcttcggcgc tgttggcttc ccattcgata ccggcgtaga tgtcttcggt
    20761 gttttcgcgg aagatcacca tgtccacttt ttctggagcc ttgaccggac tgggtacgcc
    20821 ggtgaaataa cgcaccgggc gcaggcagac gtagaggtcg agcaattgcc gcagtgccac
    20881 gttgagcgaa cggatacctc ccgaggtcgg tgtggtcaac ggtcccttga tcgacacgac
    20941 atactcgcgc accgcttcga cggtttcctc tggcagccag ttgtctgcgc catagatgcg
    21001 gttggccttc tccccggcat agacttccat ccatgcgatc cggcgcttgc ccttcgaggc
    21061 gcgttcgact gcggcgtcca ccacgcggcg catgaccggg gtgatgtcga caccggtgcc
    21121 atcaccttcg atgaacggga tgatgggctg gtcgggtacg ttaagggagc ggtcggcatt
    21181 gagcgagatg cgctggccga aatctggcag ctggatgtgc tggtagggca tgtggcttcc
    21241 tgtgtaatat atttctggaa tcgtcagccg ggccggatag ccggacaggg catcagcatc
    21301 gcccaagcat tgcaaatccg catgtaaaag tcaaaccgga gccatgcggg aaaaatttca
    21361 ttttgctgtc aacgtgtcac tcatgagccg cgttaactgc catgacgaca acgatgttgt
    21421 gtcattgatg caaaagccct tattactttc atcaacaagg atctgaacat gaacaaactg
    21481 ctctccgctc tgatcgctgc cgcgttcgct gctgtttcct tcaccgccgt ggctgccgat
    21541 gccgctgctc ctgctgccga tgctcccaag gctgaagctg ctgctcctgc caagcacaag
    21601 atgaagaagc atcacaaggc caagaaggct tccaagaaga aagaagaaaa gaaggacgaa
    21661 gctgctcctg ccgccaagta atttgcgtgc aagcaaaaag cagggattcg tccctgcttt
    21721 ttgttttttg gcgacggcat gtgaggtacg atacggcatg agcgagcata tctggaaacc
    21781 gcatgtcact gtagccgccg tcgtcgagcg tgcgggaaca tttctcctgg tggaagagca
    21841 tactgccgac ggattgcgct ggaaccagcc cgccgggcat ctggaagagg gtgaatcgtt
    21901 gctggatgcg gtcgtgcgcg aaacgctgga agaatctgcc tggcatttca cgccgcaggc
    21961 cctgctcgga gtctaccaat ggcgcgtgcc aggacaggcg cgcacttacc tgcggtttgc
    22021 cttttgcggc gcggtcggtg atcatgagtc cgcgcgggta ctcgattccg gtatcgtgcg
    22081 cgcagcctgg ctcgatgcgc aggaaatcgt tgccacaggt gaacggcacc gcagcccgct
    22141 ggtgctcgac tgcgtacgtg cctggcagag tggcgtacgc tatccgctcg atctgctgca
    22201 ccatcttgtc tgatccgtag cgcgtccaat ccattgaaca ggcgatctgc acatgtccgc
    22261 aagcggtgaa catatcgtcg tcggtatgtc cggtggcgtc gattcaagtg ttgctgcctg
    22321 gctgctcagg cagcagggct atcgcgtcac cggcgtgttc atgaaaaact gggacgacgg
    22381 tgatgacgaa cattgtccgg ccagacagga ttttctcgat gtgctggcgg ttgccgacgt
    22441 gatcggcgtc gagattgagg ccgtagactt cagcgtcgaa taccgtgagc gggtgtttgc
    22501 ctacttcctg cgtgaatacc aggcggggcg cacccccaat cccgatgtgc tgtgcaatgc
    22561 cgagatcaag ttccgcgcgt ttctcgatta tgcgctgaca ctgggcgcga cacgtattgc
    22621 caccggacat tatgccggct tgcgccagcg acacggcagg atggaattgc tgcgcgctgc
    22681 cgacgcgagc aaggaccaaa gctatttcct ctatcggctc aaccaggaac agttgtcgcg
    22741 cagcctgttc ccgctggcag cattgcccaa gacccgtgtg cgcgagatcg cacatgaaat
    22801 cggtttgccc aatgcggcca agaaagacag tacgggcatc tgtttcatcg gtgagcgacc
    22861 gttccgcgaa tttctgcaac gctacctgcc cattgccgca ggcgtgatgc gtactgcgga
    22921 aggcagggtg gtcggcgaac atcaggggtt gatgtattac accataggcc agcggcaggg
    22981 gctgggcatc ggcggtgccg gcgaaccgtg gttcgtggcc ggcaaggaca tggctcgcaa
    23041 cgaactgatc gtggtacagg ggcacgagca tccgctgctg tacagcgagc ggttgcaggc
    23101 acaggacttg agctgggtgg caggtgaagc gccagatgcc gcgttcgact acggcgccaa
    23161 gacccgttac cggcaaagcg acgcacctgc ccgcatcgct gccgtgacct cagaccgcct
    23221 cgaactgcag tttgccgcgc cgcagtgggc ggtgacttcc gggcagtctg tagtgttgta
    23281 tgcgggtgag gtatgtctgg gcggcggcat tatcgtgtga acgatgcggc tgtggcgatc
    23341 gcgcgccgca ttgcgcggtg cgtgacgtct cgtgccgccg gcccggttgc tgccgcaagt
    23401 cagtccagtc gcaggagatg ctcgcggtaa tatttgagtt ctgcgatcga gtcgtaaatg
    23461 tcggacagcg cttcatgctt gctgtccttg gagaaattct tggcgatttc gggtttccag
    23521 cgtcgcgcca gttccttgag tgtgctgacg tccagattgc ggtagtgaaa ccagtcttca
    23581 agcttcggca tgtagcgggc gagaaaacgt cggtcctggc aaatcgagtt accacacatc
    23641 ggcgaggtgc gcgtcggtac atgctgttgc atgaacgcca gcatctgccg ttcggtttcg
    23701 gcttcgtcga gcgtgctggc gcgcactttg tcgatcaggc cggacttgcc atgggtgcct
    23761 ttgttccatg aatccatgcc atcgagcacg gtgtcaggct ggtgtaccac cagcaccggg
    23821 gcctcggcca cggtttccag ctgcgcattg gtgacgacca gcgcaatttc cagaatgcgg
    23881 tcgcggtcgg gttgcaggcc cgacatttcc atgtcgatcc ataccagatt gttttgatcc
    23941 tgagccatga ttgtgtgttg cgagaggtcg aatgaaagaa accaggattg tggcataatt
    24001 cggccatgaa tacattcacc gggatatttt atgtcatgct cgcggccagc atactggtgc
    24061 gcagcgggct gatcctgcgt cagatccgtc acgtgcgccg caatcgtgag caagtgccgg
    24121 ctgcatttgc cgatcggatc gatccgatgc aacacgcgcg cgccgcagat tacagcacag
    24181 cccaagggcg gctggccctg tggcagcttg ccgtggaaac ggcggtgctg ctcggcctga
    24241 ccgtgctggg cgggattgat ctgctggcag gctggtggca gggcgtgtcg gtgccggatc
    24301 tggtgcggga cgtggggctg attgccagcg cggcgctggt tggcactatg gccgaatggc
    24361 cgctggatct gtatcgcaaa tttggcctcg aacagcgttt cggcttcaac acgatgaaat
    24421 ggagcgattt cgtgcgtgac ctgttgttgc aggccctgct ggcggcgttg ctgggcctgc
    24481 cgttgctgtt tgccgtgatg tggatcatgc gtcatgccgg agcgttgtgg tggctgtatg
    24541 tctggctggt ctgggccgga ttcaatctgc tggtgctggc gatttacccg gtgttcattg
    24601 cgccgctgtt caaccggttc gtgccgttgg aggagccggg gctgcgcagt cgcatcgaga
    24661 cgctgctggc acgttgcgga ttttcggccc agggggtgta tgtgatggac ggctcacgtc
    24721 gcagccgcca cggcaacgcc tatttcacgg ggttcggtcg cagccgccgc attgtgctgt
    24781 tcgacacgct gctgcagaac ctgttgccgg agcagatcga agcagtactg gcgcacgaac
    24841 tggggcattt ccattatcac catgtcattc gccgtctggt gtggatgttt gccttgagcc
    24901 tgctgctgtt gtggactgcg gcgttgtgtc tggacagccc gtggttctac gccgggctgc
    24961 atgtacggca tgtcggggca gccagcggcc tgttgctgtt tatcctggtg ttgccggtgt
    25021 tttcctttcc gtttcgcgcg ttggtgagca tgctgtcgcg tcgtgacgaa tttcaggccg
    25081 accggtatgc agcgcagcag gccggcgctg cggtcctgat cgaagcgctg gtgcgcctgt
    25141 accgcgacaa tgcggctacg ctgacgccgg atccatggta ttcgatgttt tacgattcac
    25201 acccgaatgc acagcagcgg gtggcgcgtt tgctggcttt ggcgcaggaa aaacagccgg
    25261 catgatgagg aggaagccga tatgaacacg tggaaattcc gtaccatcct gtttctggct
    25321 gcgtttggcg ttgtgccgct ggcagcggct gtagtcgtgg ttcctgtcaa tcctcagaac
    25381 ggcgaagcat tgtatgaaaa gaactgcatg gcgtgtcatg cggcgaaatt cggcggcgat
    25441 ggttcgcaga tctttacccg gtcttcgcgt aaagtgcaga acatgaccca gttgcaggca
    25501 cgtgttaccg catgcagcgt gaacaacaac accggttggt ttccgtctga cgaagccagc
    25561 gtggtgaact ggctgaacca gcagtattac aagttcaaat aaccgcagcc gtcgcttgcc
    25621 tggccatggc cggctggcct ctgccggttg cactggcatg cgcgtcactt gcccgttttt
    25681 gtccgggaac cctatgtcgg atatgaatca ggttcgcctc agattacaac agtttgtgca
    25741 attgcgagac tgggcacggt ttcactcgcc aaaaaatctc gtcatggcgc ttgcgggcga
    25801 aaccggtgaa ctgatcgagc attttcagtg gttgaccgag gaacagtcgt gccatttgcc
    25861 cgtggataaa cttcaggcgg tagcggacga aattgcagat gtgcagctgt atctcatcat
    25921 gcttgccgac aagctgggcc tcgacatttt cgtcgaatgc gaacgcaaga tgcgaaaaaa
    25981 tgcagagaaa tacccggtgg agcaggcaaa gggtcgggcg gacaagtata ccggactgtg
    26041 aaggcgcggg cttgcgcatc caggctgttg cgcaaacaga aggtagccgt atgttgtcaa
    26101 aaaaaaccgc ccggcagtgc cgggcggttt gtgttgtgtt acgtctggca ctgatcagtt
    26161 cacgcggcgc ttgaactcgt tggtgcgagt gtcgatttcg atcttgtcgc cgatttcaac
    26221 gaaagcggcc acagggactt catatcccgt gggcagcttg gccggtttca tgaccttgcc
    26281 ggaagtgtcg ccgcgcacgg cgggttcggt gtagatgact tcacgtacca cgatgtgggg
    26341 catttcgact gaaatggcct tgccgttgta gaacatgact tcgagcggca tgccgtcctc
    26401 gaggtagttg agggcgtcgc ccatgttctc cgcttcgatt tcatactggt tgaaatcggc
    26461 atccatgaat acatacatcg ggtcggcaaa atacgagtag gtgcaatcct tgtagtcgag
    26521 ttgcaccact tcgaactttt catcggcgcg gtatacggct tcagagcttg cgcccgtcag
    26581 caggtttttc atcttcatct tgaccaccga agcgttgcgg ccggacttgc tgaattcggc
    26641 tttctgcacc accatcggtt cgctgccgac catgaacaca ttgccggcgc gcagttcttg
    26701 tgcggttttc atatctgtga tcctgtggtt ctcaggtaaa atgtgcgata ttctacacca
    26761 tgcgggcaaa ttttgctatt tgcgtcgcga ggtcttcctg attggccaac gtggcgcacc
    26821 aggcttgcag atgcgtgagc tgggcgggca ggctggcgag ccagtccagt gcaggcagtt
    26881 cgtcatcgtc cagccggttc cacgcgtgcc agaaatggcg cacggtggtt tgcagtgtcg
    26941 gctccgtctg tgccagcatg cggtcgagca gggcatcgag cttggcggcg tgcgtgtcct
    27001 ggtcctgtgg gtaaatgttc cataccagcg gccggccggc ccagatggca cgtatcagcg
    27061 aatcttcgcc gcgtacgaaa ttggcatcgc agctccacag caaccggtcg tagtcgtgct
    27121 gggacagaaa tggcaggcgt tgcaaatgca cgttgccggt gttgccagcg gcaagccagg
    27181 cctcggctgc atcggcgagc gggctatccg gcacggcgca ccagaccggc cccggcgcgc
    27241 gcgcccagtg tgccagcgca cgtgccagcg gagcgtgggc gtaaccgaac agtgacagac
    27301 gcagcgcacc gctgggcggc ggcggacaat tgatacgttg ccagaatgcg gtttgagcag
    27361 ccggatcggc gcgaaatgcg tcgcgacgtg ccaacaggtc gtgttcgcgc agcagaccac
    27421 cgctgcgttc ggtgaagccg ggaaagaaga agtattgcac cagtggcagg cgtggatgcg
    27481 gcgagggcag ggtatggcag tcatccaccc aggcctctgc gctgaggtat tccaggttga
    27541 gccagcgtgg cggcggttgc tggcgtgcca tgcgttcgag atgtgtctcg ggcagctgac
    27601 agccgaaagc cgcgatcacc agtgcagccg gaggtgtgtc gggaaattcg gtctgtggcc
    27661 agaagcggat gtcgaccccg gcaaactgct ggcgcaaggt atcgatgcgg atcgcgggtt
    27721 ccagcgcggc cagtggccgc aggtcgttca accacagcct gaccgggaga tggtgctcgc
    27781 cagccagctg ccgtgccagt cgccagctga ctgcggcgtc gccgtaattg tcgatgacgg
    27841 tgcagaaaat ctcgcagtcg ctcaaccgcg cagccgttcg cattgctgga cgagtttgtc
    27901 gcgggtggca gagaactgct cgatgcgctg ctgctcctgc gccaccacgc tggccggtgc
    27961 gcgttcgacg aaactgcggt tggagagctt ggcctcgcat ttggcgattt cattgctgat
    28021 gcgctcgatt tccttgccca gccgtgccag ttcggcgccg cggtctattt cgacttcgag
    28081 catcaaccgg tagtcgcccg caatggccac cggagcatcc gacaccggca gcgtgggacc
    28141 caccgtcacc gccgacaacc gcccgagtgc gcgtagccag gccgacaggg gttgcagtgc
    28201 ggcggcgtca ccggcgatgg cgagcggcac tttctgtgcc ggcgacaggg tcatttcgct
    28261 gcgcagagcg cggatggtgt tcaccagttg ctgacacagg ccgatggcct ggtcggcttc
    28321 ggtatccagc agtgagagat cggcgcgcgg atagggttgc atcatgatgc tgtcgccaga
    28381 tttccctgcc agcgcagcca cgctatgcca cagttcttcg gtaatgaatg ggatgagcgg
    28441 atgcgccagg cgcagtgcgg cttcgagcac acgcaccagc gtgcggcgcg tggcacgggc
    28501 ccgttccggg cgggcgtctt gcagttccac cttggccagt tcgaggtacc agtcgcagta
    28561 ttcgtcccag acgaactcat acagtgcgcg cgcgatcatg tcgaaacggt agtcggcaaa
    28621 tgcctgggcc atgcgtgcct cggcgtgttg cagacggccg acgatccagc ggtcggcaag
    28681 gcttgggtca agcggcaggc tggcgtcgag gccggtatcc tgaccttcgc agttcatgag
    28741 cacgaagcgg gtggcgttcc acagcttgtt gcagaaattg cggtagccgt cggcgcgctg
    28801 caggtcgaat ttgatgtcgc gaccatgcgt ggccaggctg gcgaaggtga agcgcagcgc
    28861 atcggtgccg agcgcggcga agccgtccgg gtagtcggtg cgcgtctgct tttcgatggc
    28921 gcccgcctgc tgcggattca tcaggccgct ggtgcgtttg gcgatcaggc tgtcgaggtc
    28981 gatgccatcg atcaggtcga tcgggtcgat gatgttgcct ttggactttg acatcttgtt
    29041 tccgtgggcg tcacgcacca ggccggtgac gtagacttcg cggaacggga tcttgccggt
    29101 gaagtgcagt gacatcatca ccatgcgtgc gacccagaag aaaatgatgt cgaagccggt
    29161 caccagaacc gatccgggca ggaaacgctc cagttcgggt gtgtgctgcg gccagcccaa
    29221 ggtcgagaac ggccacagcg ctgaagagaa ccaggtatcg agcacgtctt cgtcctgacg
    29281 cagcttgcga ccacctgctt gcgcgcaggc ttcgtcttcg tcatgtgcga catagacgtt
    29341 gccgtcttcg tcgtaccagg ccggaatgcg gtggccccac cacagctggc gtgaaatgca
    29401 ccagtcctgg atgttttcca gccactggcg gtaagtggtg gtccagttgt ccggcacgaa
    29461 ccgcagttcg ccgcggtcga cgacgtcgag cgcgcgcgct gccagtcctt cggtggcgac
    29521 gaaccattgt tcggtcagca gcggttcgat gaccgcgtgg ctgcggtcgc cgcgcgggac
    29581 catcagtttg tgtggcttga cctgggcgag ctggttgaga tcgcgcagtt gcgcgaccag
    29641 tttctcgcgc gcgacatacc ggtcgagccc ctgatattcg gtcgggcaca ggtcgttgag
    29701 gcgagcgtcg ggcgtgaaga tggcgattgg cgccaggtgg tggcgctgac ccaccgcata
    29761 gtcgttgaaa tcgtgtgccg gcgtgatctt gacgatgccg gtgccgaaga tggggtcgac
    29821 gtagtcgtcg gcgatgaccg ggatgctgcg ttcggctacg ggaatgcgta ccgtccgacc
    29881 gatcaggtgg cggtagcgtt catcctcggg atgcacggcc agcgcgacgt cgccgaacag
    29941 cgtttccggg cgtgtggtgg ccacttcgac gaaaccgtcc gcatcatcga tggcatagcg
    30001 gatgtgccac aggaacccgt cttcttcggc ggcaatgact tcaaggtcgg agactgcggt
    30061 acccaacacc ggatcccaat tgaccaaccg cagtccgcgg tagatcagcc cttcacggta
    30121 cagacgcacg aacacttcgc gcactgctgc ggacagacca tcatccatcg tgaagcgctc
    30181 ccgctcccag tcgcacgagg tgcccatgcg acgcatttgc cgggtaatgg tggagcccga
    30241 ctgggctttc cactgccaga cccgctccac gaacgcttcg cgtcccaggt cgaggcgtga
    30301 caggccctgg gcatcgagct ggcgttccac gacgatctgt gtggcgatac ccgcatgatc
    30361 ggttccgggt tgccacagcg tatcgtcgcc gagcatgcgg tggtagcgga tcagcgcatc
    30421 catcagggtg tgctggaagg cgtgccccat gtgcagggtg ccggtgacgt tggggggtgg
    30481 cagcatgatg caccaggctg ggcggcggtc atccatgacg ggacgaaaat agccagcctg
    30541 ttcccagtac ggataccagc gcgcctcgat gtcgcgcggc tcaaaacttt tggcgagttc
    30601 catggtgcga ttgctaacgg tcaaaccgcc gattataccg gttgttggtc ggtatggacg
    30661 gaaatcccgt gttcgtgcaa ggtatcgcgt acgctggcgc gtaccagtgt ggcgatctgc
    30721 tcctgcatca ggtgcaggct ttcgctcaga gcgtcatcga ctgcctgtcg caccaacgcg
    30781 tgggcgaggg gctggaattc gtcatgcagc cgggtttcga tgcacagcat gatatggtgg
    30841 gccatctggt cgagcgctgc ggcgctgacc gattgtgccg gctgtgctgc agtgggctgg
    30901 cgggttgccg ccacgctgtc ggttgcgtcg gttgcgtcgg tatgcggttg cggtgccgca
    30961 tcgcccagtc gcaccagatc ggtcagtacc ggcagttcgt ctgcggcggt cggaggggag
    31021 ggcggcggcg caaccggcgg caacggcgtc tggtacttgg ccatcagttg ctgcattttg
    31081 tcgaacaagg gttcgctcat cgtacagatg ccatgtcgtg gttgtgcagg gcatagccac
    31141 gttgctggta aaaacgccag cgtacgcgtg cggcctgccg atccggttcg tccgtggtaa
    31201 cgatttcgat caatcgttcg aagcgcgtga aaaatcccgg cgtctgttcc gacaggttga
    31261 tcagcacatc gcaatgcggc aagtcgccga tggcgctgcc gagcacgaca ggggactgcg
    31321 gggcatgcgt cgcgtgcgcg cgcgcgtggg gcagaaaagc actgggcgga tgcgtccacc
    31381 agtgccggtc cagtgcgtcg agcagtgcct cattgtggct gtgcaccagc acgcgcgacc
    31441 cctggcgcca ggccttgacc gcaatctggc gcgcgagctc gctcttgtcg tctacgccgg
    31501 tgtagaaatc cacccgtgtc atttcgcagc ggcgtgggcc gcaaggaaat gcaccagcag
    31561 tggcaccgga cgtccggtac tgcctttttc cttgccggat ttccaggcgg tgccggcgat
    31621 atcgaggtgt gcccagcggt agtttttcgt gaagcgtgac agaaagcagg ccgcggtgac
    31681 cgagccggcc gggcgaccgc caatattgcc gatatcggca aaattgctgt cgagctgact
    31741 ctggtagtcg tcccacagcg gcatgcgcca ggtgcggtcg tgcgcggcgt tgcccgcccg
    31801 ttcgatttcg gccgccagtg ggtcgtcgtt gctgaacagc ccgctggcgt ggtggcccag
    31861 cgcaatcaca caggcgccgg tcagtgtggc gatgtcgatt accagttccg ggtggaagcg
    31921 ttcgacatag gtcagggcat cacacaggat caaccggcct tcggcatcgg tattgaggat
    31981 ttcgatggtc tttcccgaca tcgaggtgac aatgtcgccg ggtttgattg ccaggccatc
    32041 gggcagattc tcgcaactcg ggatgacgcc gatgacattg agcggcagct tgagctgggc
    32101 acatgcgcgc agcgtaccca gcacgctggc ggcaccgctc atgtcgtatt tcatttcatc
    32161 catttcggcc gccggcttga gcgagatgcc gccggcatcg aaggtgatgc ccttgccgac
    32221 cagcaccacc ggagcagcgc cacgcttgcc c
//
LOCUS       DAIANL010000130        27950 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2201663, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000130 DAIANL010000000
VERSION     DAIANL010000130.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 27950)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27950)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27950
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2201663"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         6075 a         8799 c         7586 g         5490 t
ORIGIN      
        1 ggccgccgat ctgcgcgccg attatcgcaa acgctatccg ctcgtgctca cggtcatgaa
       61 aggcggcatg gtattttccg gctggttgct gccgcgcctg gattttccgc tcagttgcga
      121 ctacatccac gccacacggt accgcaacac cacccgcggc gacacgctcg aatggcttgc
      181 aatgccggcc gaacccgtgg ctggtcgcca cgtgctgctg cttgacgaca tcctcgacga
      241 aggccatacc ctggctgcca tcaagacccg gttgctggag cttggcgccg cacacgtcgc
      301 atgtgcggtg ctgactgaca aacgtaccgg cacggacaag ccgctccagg cagaatatgt
      361 cggtatcgaa gtacccaacc gctacatttt cggctgtggc atggacatcg aaggcgcatg
      421 gcgcaatcta ccagccattt acgccctgcc agagccaccc ccactgtaac agcctgcaga
      481 aatatgtgac aattgccgcg cccggattac cccgggtgca tgtaacctct aaccagacca
      541 aggatctcat catgctggcc attatcggcg gcaccggcct cacgcagctt gccaatcttg
      601 ccatcactca ccgacaggtt gcacgcacgc cctacggcga gccgtccggc gcactgacct
      661 tcggcgaaat cagcggacac caggtcatct tcctggcccg ccacggctac ggacacacca
      721 ttccaccgca ccgcgtcaac tatcctgcca acctctgggc gctcaaacac cacggcacaa
      781 cgcacattgt ttccgtggcc agcgtgggtg gcattcaccc cgacctgatt cctggaacgc
      841 tggtcatccc gcaccagatc atcgattaca cccatggccg caaagcgagt tttttcaacg
      901 gcgaagaaca gagcgtggtt cacatcgact tcaccgaacc gtactgcgcc accatgcgtc
      961 aacgtatcct cgatgcggcc agaagcgctg acgaaccgtg tgtcgattcg ggcgtctatg
     1021 cgtgcgtgca gggaccgcgc ctggaaaccg ccgccgaaat caatcggctc gaacgcgatg
     1081 gtgcgagcat ggtcggcatg accggcatgc ccgaagccag tctggcccgt gaactcgagc
     1141 tcaactatgc cgccatcggc gtcgtatcca actacgctgc cggccgcggc accagcaaac
     1201 gcgccatcga tccggccgaa gtgcagaaca accttgccat gaccatggag cgggtgcgca
     1261 aggtactcga gcacctggtc gcactgcatc acgaaagctg agcatgaggt atgccagcgg
     1321 ccggtgtgat aacggatgtt tctgtttggg acaaagcgga cattgccatt aggagatgac
     1381 acaccatctt gaccgaaaaa gctatccatg atgtaatacg cagcttcgtt atgggcggtt
     1441 agctcagcgg tagagcactg ccttcacacg gcaggggcca ctggttcgat cccagtaccg
     1501 cccaccaata aaatcagtaa gttacgaatc aaaatccgga atagaacgat tcaggtgtaa
     1561 atcccggtgt aaatcggagt tacctgcgcc tgatccacgc caggtaatcc ggcgcctgcc
     1621 cgacattagc gaaccactga atgcggcgat ctggccgtgc gcctagcgtg ataaccgcag
     1681 tcatcgaagc cccatattgc gctggatagc aaagttctcg gcatatctgt cgatgtacct
     1741 gtgcccgcgc tgtgcaaggc tttcagcggc atgctgccga aaagctgccg aacttcttgc
     1801 tcacctgctg ggtagtagtt ttgcatgccc cgcaaacccg tcacctgcgc acacaaaaaa
     1861 tacaatcaaa attgtttaaa tattgcttgg cacattaaac aaaattgttt aacatgccac
     1921 atgttcaaca aacatcgagg ggaaggtgaa gcaaagcgaa ttcgtaaggt ggttgaccaa
     1981 acaaggtgcg actttcaaag acggtagcaa gcatttgaag gttatcttga acggtaaaac
     2041 aagccaccta ccaaggcacc ccggagccga actgaagaca ggattggttg aaggggtaaa
     2101 aaagcaactg aacctcaaat gaaggcaagc cccgcaaggg gcttgaaggc tttttacgaa
     2161 tttcgcttca ccatcccaaa aggagattga tatgaaatac ccggcgacat tcacaccagc
     2221 cattgaaggc ggttttgtgg tcacatttcg ggacattcca gaggccatca ctcaaggcga
     2281 tacggaagag gaagccacgt ccatggcaga agacgttttg ctgtcatcca tggaggtcta
     2341 ttttgacgaa aaacgtgcga ttcccgcccc atccgcactg tcaccggacg atcatcccat
     2401 cggcattcct gccagcgttg ccgcgaaagt gctactgctg aatgaaatgc tggcgcaaaa
     2461 cgttactgca tctgaactgg ccaggcgcat gggaactcgc ccgcaagaca tcaaccggat
     2521 tatcgatctg aaacacgcca cgaaaattga caccctggcc aatgcgatag cagccatggg
     2581 aaaaagcctg gaaattctgg ctgtttgagt gtggctaggc cggtggaagc cactggttcg
     2641 atcccagtac cgcccaccag agaacacgat ggcccaggtt aattcccagg ccatttttgt
     2701 cgattcggga tgttttctgg acaccaatcg cccaaccgct tgccgcagcg taacaattcg
     2761 cggcatgaat tgagcaggtg tcgcaaggtc attacagcaa gtccctgacg cagccgaaaa
     2821 cacaataaat cagtaataca agccggcatg atgctccccg gcccgtttct gccccaacgc
     2881 cattggcacc cgtggcacat caccatgtgc aatgctgcac tgtacctgac tcagggaaca
     2941 gcggggcgat tgatacgtca gatacctgat ttctgcctcc gctcactcag aacttcgctg
     3001 tcaccatggc ccacatggcg gtggtgtttt ccagaccggc aggcttgccg ggctgaagat
     3061 cggcataaga agccgcgccc gacgcattga ccgtaaaatc agccaactgt acgacaaaat
     3121 cgatgttgcg cgccaacggg tgcccgatgg accaatccca ttcatgcccc aggtcggtac
     3181 cctgataatc ggtagcgaac cggtaatacc ggaccagaac gtcgaagccc cacacattct
     3241 cgtcatctat cgtagcgtaa ttgctgcgca agccttccct gggtgtagtc agaaacattt
     3301 ccgcccagcc gttgaacgca tgtttggtag ccattggtgt ctgaaaacca tatgttccat
     3361 tcgcgttcgt acccatgcgc atgtagtcgg cagagacata aatcgttccc caatacaacg
     3421 tgccatcgaa atgatcaaaa tcgttaccga tcgcactgta gccaccatcc caggaattct
     3481 ggcgtgcata ttccgccaca tagctggctt tccaggcatc ggcgagcgga atgtccccat
     3541 gcaggcgcat gcccagcgtc gcgctattgc acgcctgggg gtcaaccaga ctgttggcac
     3601 tgcctggccc tgcaccgcac agcgctgctc cgggaacgat ggcatcgttc ccgctcacgg
     3661 tgctgccgcc agcgttttcc tgattgccat accagtaacc gaaggcgtcg atgtgcgcga
     3721 acgcataacg tgtccagtcg gcctcggcaa gaaacgtgtt catgttcact tcctgatcga
     3781 gcacattctt ctcgccgaaa agatatgcgc ccataatcct gaaatcagac tgcgggtgat
     3841 tgaccaggct caggccatca taggtctgca tgttctgccg gaaacccaca ttgccaacga
     3901 agcgctgatc atcaagttcg atcagctgcc gtcctgcgag aatgcgggta tgggcgaaac
     3961 catcgtaaga cacgtaggcc tgattgaaat tggaagcaac aggatcgaga atcgtagcat
     4021 acgcaggatt gctaacccgt ggcagcggtt cgcccggcac gccataagtt gcagtatctg
     4081 ccgcagcaac accgatcagt tccagcgtag cgccgaaacc acccgcatca ggcgtggtgt
     4141 acccggccag aacgcgcgca gtcagcgctt gtgcctcctg tttcccctgc tgctgcacaa
     4201 cctcgtagcg cggacgaaac tcaaaattgg ttactccgct gctgcccaca ttggcgggat
     4261 tgcccacgac catggtatcg gcgacggcag ttctacaata tacggttaca acgaacagca
     4321 acacaactgc cggcttgaaa ccggttgcag actttgaaat tccagacata tttccccaac
     4381 ccgcccatca tggacaatca ccgctggcgt tcagccttgc acccgccgca cgatttcaat
     4441 tcatgcccat catgccggcc aatggccgtc ggaaaaatca gcacaggacg attttcaggt
     4501 cagaaaaact gcacttgggg tagggatttc ctgaacacac ctcagggtaa ctggcgaacg
     4561 gggccgaacg ctgcatggca gcgaccggca cacccatttg atgtcgcact aggcagcaat
     4621 ggtgtctatc tgccgcgcaa ctgttgtgtc acgctgagga cgcgctcgcc aaacaggcgg
     4681 ccggtttcca ggtcaccgtc gtccatttca gctacgctgg catcggatgg cgtctggctc
     4741 atggcaccgg cagacgaacc gacataattg atgtcactgc gctgcgctgc cttgctgttg
     4801 gacggcatca tccccgtgcc gacccagatg ccgccgtgtt gcatggccag agtgaacaat
     4861 gaatgcaacg tagacagttt gtcgccattc atggttgcgg aattggtgaa gccgccaaac
     4921 accttgtcct tccacccttg ggtgaaccag cgacctgaac tggcatcggc aaatttcttg
     4981 aactgccagc tcacgctgcc catgtaggtc ggggtgccga aaacaatggc atcggccgca
     5041 tccagcaaag cccagtcagc ttcactgaga ttgccttcgg catcgatggc tgccaactgc
     5101 gcccgtgccc cctgcgccac cgattccgca agcctgcggg tgtgtccgta accgctatga
     5161 tagacgacca ctgtttgtgc catatcgctc tcctgaataa attcccggac gtcttgcgtc
     5221 cgtaaaaggc cggcgttcct gtctcccggc agcatgaacg ccacctgttg aaaaaagatt
     5281 acgcgactgc accattcaat catttattca tcagatgaat aagtataata attcgcttca
     5341 aacaatctgt tttttgaatc aatgcccccc gacatttccc tggaacaatg gcgatcactg
     5401 attgcagtcg tggaaagcgg cagctacgcc cgtgccgcca tacagctgtt caaaagccag
     5461 tccgcagtca cctacgcagt gcaaaagatc gaatcgctgc tgaatataaa gctgttcacc
     5521 atgagagggc gcaaggcgat actcactccc atcggtcaga tgctgtaccg gcgcgcagtc
     5581 gcgctggtcg acgaagccag cgatctcgaa accgctgccc acaccctgtc tgccggctgg
     5641 gaagcggtga ttcatctggc tgtggaaatc ctgtttccca ccccagtgct gctggaatgc
     5701 ctgggacgtt tcagtcagac cagcccgcgc acccgcatcg aactggtcga gtcagtgctg
     5761 ggcggcacga ccgatgcgct gctgcacggc ggggcagatc tggccatctc accccatctg
     5821 ccggcagggt atccgggcaa cctgctcatg catgcacgag tggtggcggt ggcgcacgcc
     5881 gatcatccac tgcatcatct cgatcacgca ctaacactcc gtgagctacg ggcgtatcgt
     5941 cacattgtcg tacgcgattc aggcagcaac cgcgatgaac gccagatcac cacggtagac
     6001 gtcgatcagc gctggacggt aagcaacctg tccacttcca tcgaggcggt agccatgcaa
     6061 caggggtttg cctggctgcc cgaaacgcat attcatgcgc ggctgcaatc gggcgaactg
     6121 cgtccgttac ctttgcgcga gggcggcatc cgcgagattc cgctctatct ggtcctggcg
     6181 catcccgacg tggccggtcc gggaacacga catctggccg agaccatctg cgctacagtc
     6241 agcagcatga atgaacgggt aaccacacaa ccgtctcctg acggcaacat cagataacgc
     6301 acaccgggtg gactccggcc acctcagctg gtcggcccag atggtagcct tgggcaaaat
     6361 ccacccccat gtcggtgagc agcgccagtg tggcctcgtc ttccacccat tcggcaatgg
     6421 tcttgatttt catcacatgg ccgatttcgg tgatcgcatc gaccatggca cggtcgatcg
     6481 ggtcggtcac catgtccttg acaaagctgc catcaatctt gatgaaatcc accggaagat
     6541 ttttcaggta cccaaatgaa ctcaggccac tgccaaaatc gtccagcgaa aacttgcagc
     6601 cgagcttttt cagttcgcga ataaatgtcg tcgctttgct cagattactg atggcaacgg
     6661 tttcagtaat ctcgaaacag atctggctga aatcaatgcc aaagcttttc cctgtatcct
     6721 gaatgaacgc cagcacgctt tcgtcgctca gggtcgatcc ggacaggttg atcgccaaca
     6781 gacgcctgcc atctgccccg atgccaaatg cccccgcgga aagcgcggaa aacacgttgt
     6841 agattaccca acgatcgatg cgttgcatct tgttgtagcg ttcggcggca gacacaaaaa
     6901 agttgggcag gatgatgccc ccatggccgt ccctcatacg cagcaggact tcccagtggt
     6961 gcgcctcacc tgctgcgtcg gacaatgcac agatcggttg ttgatacagc acgaaacggc
     7021 cctcttccag cgcctgcgcg atcaggccga tccagtgcat ctcaccgtga cgctgcagca
     7081 gcacatcatc agttttttca tacacatgca cccggttgcg accgctgtcc ttggcggcat
     7141 agcaggccag gtcggctgag ctcatgatcg tcgaggcgtt gaggttatcg gcattgattt
     7201 ccaccacgcc gatactggca ccgatttcga acacgctgcc tatccaggca aaacggaagt
     7261 ccttgaccgc ctggcggatc ttctcggcgg tttcgatcgc accagccaga tcgcaattaa
     7321 tgagcagtat gccgaattcg tcgcctccca gccgggcaag cacatccgaa cgccgaatgc
     7381 attcgagcag cacctgcgcc aattggcgca acagttcatc tccggccgta tgaccacacg
     7441 tgtcattgac caccttgaac tgatccaggt cgatatacag tagcgcatgt gtgtccggcg
     7501 gacggatctc gtcgagcaat gcgtcaagcc gctgttcgaa cgcactgcgg ttcaccagat
     7561 tggtcagtga atcgtggctg acctggtgcg acagctgcat ggccagccgg cgcgtatggc
     7621 tcacgtccga acacaccatg acggtgccaa caacctctcc atcggcattt cgcatcggcg
     7681 ctgcggcagc atccactgaa accaggtgat catcccgcag caacagcgcg gcgttttctg
     7741 ccagatgaac cgtggtgttc tgttgcaggc actccgtcac cggattaggt aaggccaggc
     7801 ggctgatttc gtcaatcagc gtcaccacct cgagcaccgc atgcccacgc gcctcctcca
     7861 gcggccagcc agtcatcttt tccgccaccg gattcaggta atccaccaca ccgcgggcat
     7921 cggtggaaat caccgcttcg gacagcgaat tcagcgtcac agccatgcgt tctttctgcg
     7981 ccagcaacgc agcttcagat tgggtagcgc gtttcagcgc atccatgagt tcggcatatt
     8041 gccttgcacg actgtcaagc gcacggttga tgaatttcga caggaagcca aattcgtcat
     8101 cccggtttgc atcgaagcgc acggcggtat ccccatcggc aaattgctgc gcggtgtgta
     8161 ccatacgggc aaacggctgc gataacagtc gttgcagaat gaattgcaga atcaaaccga
     8221 acaccatcat cagcaaggcg atcgacagca gtatccattt gcgataagtc gaaatcagct
     8281 gttgcaggct ggggaaatag atcgacgtct gtaccgaata cggtgcgaga tgcgcataca
     8341 cgggtcgaat ggcaagcccg gcagcaatcg cgtcttcgtt gccgctcttg cgtccgaaca
     8401 ggaactgccg gttgccgcgg cgaatttcga tggcatcgta atgaatgatg ttgcggaacg
     8461 gttgcaggtt gtgttccatg tcgcgccgcg tcgtttcatc gaaatccgga ctgccggaat
     8521 attccagtga tttcgtgatg acggtctgca ggatctttgc gttgtcacgg tactgcaggg
     8581 ccaactcgct ttccttatcc ttcaggatga tgaaagcaat cagcaacccg atcagaaccg
     8641 tgccccagaa cacaattccg gatatttttg tgggcaggtt actggccggc cccggcgaat
     8701 tcggttcctc cgtcattgcg ttacctgaaa ccgatgcgtt tgagactgaa cgcccccgga
     8761 cagatcccgc tcacgccggc gccaagaatg gcaaatgcca tgaaccacgg cacgaaccac
     8821 agtttctgat agaaaaaccc ataggtgatc agcagcaaca cccccaccag caagcgccag
     8881 gctcgttctg cctcgaactg gaagcgcacc ttatactgat cggaaacgca cggcctggaa
     8941 gagaatagtc ggggaaacag ccggctgacc aaaatcggca ttcgccagtt ggtcaaccct
     9001 tcgaatccca acatcacgat caacgaatac atcagcaggt tcaggtcaaa caccagtgct
     9061 gccaacatca acgcgcccag aaacgttctg tacatacgat cactcatgcc tcactccagc
     9121 cttgcggcga tcccgcacgg cacccggtca cccagatttt ccgagcaccc gcctcgatta
     9181 ttgttatcgt catgcagcca gtcatgttat ggattttgtc atgacagacc ccatcgagac
     9241 agagtagacc caaactgaaa tccggcgtca agccttttgt aaaaaaattg ccacaatgct
     9301 gtttgccgct gccggtgtca tcccgcagcc cagccgagtt tgagccaacg gtattgattt
     9361 ttattccttt atggcgcgac aatgcacaca tagttgcccg gtgtacaact catcggtcag
     9421 gggcaatccg tgtctgcccc gcatgctgca ttgcttcgga gaccacaatg tccgaaccgt
     9481 tgtcgcagat ttgcccgcca gactcgcccg aggcgctgtt ccgcattttc atggcggaat
     9541 gccaggacgg gatcttcgcc accgacacgc aaggtgtgat cctgatggcg aaccgcagcg
     9601 cggaacactg gctgtttcat aacgccagcc ccgtatccgg ccggcatttc ggcgaactgt
     9661 tcgacccgat gcaaaccgcc accctgaaca caatgctcga tgccatgcac cacggcatcc
     9721 gcccgccatt acgcgaactg cttgtcgacg gcgcgcaggg aaccatcctc accgtacaac
     9781 tgtccgcctt cccggtgcat gacgacagcg gcgtgcttgt cggcgccgga tttgttgccc
     9841 gagacatcac acgcagcaaa caggtcgagg ccgaactcga cttcgaacgc aaccggttcc
     9901 gcctggcggt cgaggccgca ccgctggcac tgctgatggt tgacgagaat ggccggatcg
     9961 aactggcgaa caaagcgttg gaaacaatgt tcggtttcag tgctgacgaa atgatcggac
    10021 agtccatgga aatgctgctg ccagaccacc tgcgcgccca ccatgccgga ctgagacacg
    10081 attttttcag caagcccgcg tttcgtcagc tcggccacgg ccgggaactg ctcggacaga
    10141 cgcgcagcgg cgcgctgatc cagctcgaaa tcgctctggc accgattcgc acccacagcg
    10201 gaaacgctgt cattgcggcc atcgccgaca tttcggagcg caagacacag ctgaatgcgc
    10261 tgcacgaacg cgaagtgcgc taccgttcgg tgatcgaatc cgtgccggat ggatttctca
    10321 tcatccaccg cgacggacgc atcaccgtat ccaaccccgc ctacagccgc atgtcgggct
    10381 acagccgcag ggaattgctg caactcaacg tggcgacact caacgcgacg gacaatcaac
    10441 agctgatcac caatcgcatc cagaacgtac tgctgcaagg ctatgaacgc ttcgagaccg
    10501 cgcaccggcg caaggacggc agcgtctggc cggtcgaagt caccgtctgc ctgatcccat
    10561 ctctcgatga gctttttgtt ttcgtgcgcg acctgaccga actgaagctg ctggaagaag
    10621 aacgcagaca ggccgaacag caaatccgca tcatggcctg gcacgactat ctcacccggc
    10681 tacccaaccg ctggctgctg atcgaccggc tgagacaggc actctcggcc gcacgcagaa
    10741 accatactta cggcgcactg ctgttcatcg acatcgatca cttcaagtgg ctcaacgaca
    10801 gcctggggca cgaaatgggc gacctgttgc tgatacaggt gtcgcgacgc ctgctggaga
    10861 acattcgggc agaagatacc gccgcgcgct ttggtggcga cgaattcgta gtcatgctca
    10921 ctggcctgtc ggacaacatg gccacagcac tggagcaggg tcgacagacc ggaaacaagc
    10981 tgctgcgcgc gctcaacatt ccctatcagc tcagaacaca tctgtatcgc tgcacaccca
    11041 gcatcggcat caccctgttt caggacgatg aggacactga tgccattttc cgccgtgccg
    11101 accgcgccat gtatctggtg aagagttccg gacgcaatgc cgtgcacatg cacgaacatg
    11161 ccgcatgaac gattctcctg atctggcccg caacgcggcg catggagaat tgatcaccgc
    11221 gtccctagcc gccagtaggc agcggcgcga cttcggcgag aaactggctg aactcgcgcc
    11281 aggatgcggc atctgccagc gcatcgtagg ccagccccgt ggatgggtcg ctgcctgcaa
    11341 gcgggctggt gaatgcatgc accgcgccag ggtaggcaat gaaacgcagg tcgatacccg
    11401 cctgttgcat ctcgttgcga aacgtcgtca cctcggcctg cggaacgaac ggatcggctg
    11461 cgccatgcat gaccagcacc ttgccgcgaa tgttggcgac gtcccgtggg ttcgggttgg
    11521 acaacccgcc atgaaaactc accacgccgc tcagttcggc gccactacgc gccagctcga
    11581 gcacggtcgt tccgccaaaa cagtatccca tggcgaccag atactgttca tctacctgcg
    11641 gcaaggcacg caaagcatca taaccggcac gcacccgcat gcgcagggca gcccgatctt
    11701 cgcggaacgc ccgcgtcatc ccggccgctt cctgtgccgt tgccggccgg attcccttgc
    11761 catagacatc ggccaccagt acaacatagc ccagtttggc cagcgcttca gccctggtgc
    11821 gcacaaaatc gcccacaccg aaccagtcag gtgcaatgag gatacctggg cgcttcagtc
    11881 cggattccgc cggcccggcc aggtatcctt cacacagggt gccgtcggca gcaagatagc
    11941 tcatcacttt tgtgaacacg cgcgtcatct tgtcctccag taacgcagta acggtagcac
    12001 tccgaccgca ccgggccaga gcaccatcat ccagatccat gaacgcccat gcaacgccct
    12061 cggcgcaggc cccggtacgg aaaaacatca tccaggcaaa cgatccatca ccccggcaac
    12121 cggtaacacg ctgcccatat cgtatgtgtt tcccataccc ggccgagcgg cccagcgtat
    12181 catggtcacc tttccagaca cttacatgac aatgcctgac agcagcatgc cttacaggac
    12241 acgcggcgcg acagcgcatc cccagcccag gacacaatat ccctgtccgg aacatgacat
    12301 atatgtgcac tatgtttaca atacccgggc agtccggtcg ttggtccctt tcattctata
    12361 atttgtgccg acatggatga caccccgatc tttcgaacgc ttttcgaagc cagcggcgaa
    12421 gccatcggac tggccgatgc agaaactggc caaattctcg atatcaatcc ggcactggag
    12481 cgcttgtccg ggtggcccag ggaagaactg gtcggcaagc cgcaacacgt gctgcacccg
    12541 ccagagcccg gccagccctt cagccccagt ttttcccggc atcgcgcagc cactaccggc
    12601 tcgaccattg aaaccatcct gttgcaccgt aacggtctga atatcgacat ccagatccac
    12661 gcctcacccg tcatactcga tggacgccgc gtgctactgg gtattttccg cgacatcacc
    12721 cgggaaaaac ggctggaacg cgccgcactc ctgttatcgc aatgcgaccg catcatcatg
    12781 accgccgaca gcgcttccgg catgcaacag gccatctgcg accagatcgc ctccacggcc
    12841 ggctaccgcc tggtatggat tggcgaggcc ttgctcgacg cagacaaaag catccgcatc
    12901 ctgtgccatg cgggccacaa cgaaggttac ctgcagcacc tgcatctgag ctggggcgaa
    12961 ggcgtctgga gcaacggccc atccggcctc gccatcaaaa gcggacgccc gcagatcaac
    13021 cagaatttcc tggccgatcg gcagatgtcg ccgtggcgcg aactggccga gcgcaaccag
    13081 ctgcgtgcca gtatggcgct gcccatcgcc aatgccccgg accagacgct ggtgctgacc
    13141 ttatatgccg aaaccagcaa tgccttcgac gcagacgagg tgcgcctgct ggaagacctg
    13201 gcgcacaagg tctcctacgg acttggcatc ctgcgtgcac gcgaggaaaa ccgtcaggtc
    13261 ggcatgcagc tcgaactggc caacaccgtg ttcaaatcca gccacgaagg catggtggtc
    13321 accaactacg ccggcgaggt gctgtcggtc aaccccgctt tcaccgccat caccggctac
    13381 ccggttgaag acgtgctggg ccacaagctc aaaaacctgc tcaagtccgg acgtcacaac
    13441 gccaactttt accgcacgat gtggcacgaa ctcgaaacga gcggacactg ggaaggcgaa
    13501 gtgcacgacc agcacaggaa cggccaacca ctgacgctgt ggttgagcat cagcgccgta
    13561 cccggctatg accgctcgct gacggatgca cagtctccct gccacatcgc actgtaccgc
    13621 gacatttccg aacgcaagca gtttgacgcc accatgtggc gcgtcatgaa cttcgacgca
    13681 ttgaccggcc tgcccaaccg taacctgctc cgcgaccggc tggaacagga tgtgctcaac
    13741 gctcaacaag gtggtggcat ctgcgggctg atgctgattg atctggacca cttcaagctg
    13801 gtcaatgatt cgctcggtca cggcgccggc gacagtatcc tcaaggaagc ggcccggcgc
    13861 atccgcagca tcgcccgtcc gtccgacacc gtggcctaca tgggcgcgga cgagttcgcc
    13921 atggccgtct ccacgctacg cgacatccag accctggacg ccatggccca gtctctgctc
    13981 gaggcgctgc gccatagttg gcagatcaac aatgaaaaat tctcgctcgg cgcaagtatt
    14041 ggcgttgcta catggcccgc tgacgcagac aatgtttccg agctgctgca tcgcgccgat
    14101 ctggccatgt accaggccaa agcgggcggc cgcaaccgca tccattactt cacacccgac
    14161 ctgcagcagg aaagccggga gcgtttcgaa ctgacgcgtg acctgcacga ggccatgacc
    14221 agcgcgcagt ttgtcctgca ctaccagccc gtcatcgacc tcgccactgg cgagattgtc
    14281 aaggccgagg cactgatccg ctggcagcat ccgcaacgcg gcatggtcag tccgatgcag
    14341 ttcatcccgc tggcggaatc cagcggcctg atccatgctc tgggcaactg ggtgttcgag
    14401 gaagccctgc gggtcggccg cagctggcag caacagtacg gccggatgat ccagatcggc
    14461 atcaacgttt cgccagtcca gtttaccgca gatgaattcc cgcaccactg gctacggcag
    14521 ctgcaacaag ccggccattc cggcgaacag atcaatatcg agatcaccga aggcctgttg
    14581 ctcaaggatc gcccgggcac gctgcttagc ctgaaggcgt tgcgcgaccg ccaggtagcc
    14641 atcgccatcg acgatttcgg caccggctat tcttcgctgt cctacctggg ccagtttccc
    14701 atcgatttcc tcaagatcga ccaatcgttc acgcgcaacc tgcaacccgg ttcaagtgaa
    14761 ctggcgctca ccgaggcgat catcgccatg gcgcgcaagc tcggactcag agtcatcgcc
    14821 gagggtgtcg aaaccatgca gcagctcgac ctgctcatcg ccgccggatg cgactacggt
    14881 cagggctacc tgttctcgcg cccggttccg gctgacgcct ttgaacaact gctggtcgaa
    14941 ggtgtcgcac acgctacttc agcgcctcgt tgagcactcc ggccagtcgc acactcgccc
    15001 gttcgagctg cccctgtgct accggccagg cccaggattg ataaccgttc tcccagcccg
    15061 gctccgccac tggcggcagg tgccgataca ctgcgcacgc cagcgcgtgc gaaccgttcg
    15121 cccacgcctg cacccgcaga tcaatctgtg ccacggcgcc agccggcgcc cactgttccc
    15181 gctgttgcgc cgtgatcacc gcatccagcc gcgccgcctc ggcacgcacc gcctcatgat
    15241 cgaaactgta acgcggccca agctgccaac cgaaccggtg ttcgatgatg ccgaggtccc
    15301 agatcgcatg caggttggac ggctgaccat accacggcaa ccgcacatca ttgccgccgc
    15361 ggtcatgatc atcttccgca tgcaacggct ggtggatatc agctaccaga tgcaccaccc
    15421 acttcagcgc caccagccgc ctcgcatcgc cctgcgacgg atccgccaac atgcgcacga
    15481 attcaggcag cttcgccacc acacatgtca tctcagcgac ccgctgccca tgttcgtcaa
    15541 catgacaatc ccgttgtgcc gaatacgcca cggcatacag cggaatgtcc acgtaatgcc
    15601 acggcccggt ggtcggctcg gcactgcgta tggcatccgg ccacgacgac acctgatcca
    15661 gccggcgata gccctcgacc gacaacaacc ggcgcacctc ggacgctgcc gccggactca
    15721 gatgcgcctg agccatgtcg gccaccagcg catgcccttc cggtccccat gcccacgcca
    15781 ttcccgcctg caccaggcac ccactcgcca ccatcatcaa cgccacccgg cgcgccacgt
    15841 ttccagccat gtcgcaaccc ctgatttata cgcaatgaag tagcataccg cgtccagatg
    15901 acaaaccgca cacgctctgg cccgcagcaa ccgaaacaaa accggcaaaa ggggcttgag
    15961 cgaacatcca gccggtgtta gaatgcacgt cttcgcgaaa cagcacgcgc ccgtagctca
    16021 gctggataga gtacttggct acgaaccaag gggtcaggag ttcgaatctc ttcgggcgcg
    16081 ccaatccgat acgaatctgg gcactccaac tggagcgccc attttcattt ctgacaccgc
    16141 attttccagc gcagcggtgc tgccacgcag ggtagcactc tgcgtatcca cccggatttc
    16201 atctaccagc aggtgcagca actgcttgcc gtagccctgt tcgggatcaa gcagcatctg
    16261 ccgcatgcgt tcggtggcgt aggccacttg cttggacgaa accttgcgga aactgggctt
    16321 gtctcgttgg taatcccccc atttttagtt ggggtcaaaa gtagattcat gcgaccatgg
    16381 ccagccgctg tttcggtgtg gcttcgacca agccttgcca caggctggat gatatcttct
    16441 gatagacata caagcaaaaa agggttaaat tatcaccagg tattaccctg tgatattggc
    16501 tccagtatgg caacctctct caaaatcgat gatgtactga aaagccgcgt tcaacacctg
    16561 gccagccaac gccgtcgctc gtctcactgg atcatgctcg aagccatcca gcaatacgtc
    16621 gagcgtgaag aggcgcacga gcgattcaag caggaggcta tggcatcctg ggcaagttat
    16681 caggagacag gccgtcactt gactgggcag gaagttcgtt cctggctgaa cacctggggc
    16741 accgatgacg aaggaacggt gcctgagtgc cacgagtaat tgttacacaa ggcgcgatgg
    16801 aagggttgga acgctgcagg cagttccttg ccgtcagatc cccggaagca gccagacggg
    16861 ccggtcaggc catcgaacga caatttttgc tgctggaaac agctcctgac attggacggc
    16921 cgtttcctga tctccctgaa ctgcgtgaac tgatgatctc tttcggagac tccggctatg
    16981 tggcactgta tcgccacgaa ccggttcgcg atgcggtgta tgttctcgct ttccggcctc
    17041 agaaagaggc tggctactga ttgaagatcg agatgctttt gccgaatcgg aatcatgttc
    17101 aattcatatt gccccagcct ggatcagact ggcatgacac agggcggaag cgtcacaagt
    17161 tgttgtatat taataaataa atttcaattt tggccccact acgaaccaag gggtcaggag
    17221 ttcgaatctc ttcgggcgcg ccaatccgat acgaatctgg gcactccaac tggagcgcca
    17281 attttcattt ctgacaccgc attttccagc gcggcggtgc tgccgcgcag ggtggcactc
    17341 cgcgtatcca cccggatttc atccaccagc aggtgcagca actgcttgcc gtatccctcc
    17401 gccaaccggc tggtcaaccg tgttgcgcac gggttgcgtt gctcccgata tcaggaaagt
    17461 tgtcgcttca accctctgac aacagctgca tcccaacgat acgagggtcg ttttcctgat
    17521 gagtcaagtt ttcagcattg tcagttgcgt agtatagaat ggctcgaccg ggccgatgtc
    17581 caccattgcc ccaacgggac cagcggcagc ctggaagcgt ggatcgaggt gggaattttc
    17641 atgatcccac actgactccc atatgccgtg catgatgtag gcgcgctgcg ccccatcctg
    17701 ggtagccttg cgcaagattt ctgtactcac ggcctttcgg tagaagcggt tatctgccga
    17761 gcccgcgtgg ccgatgccat ccgcagcact ggtgggctgg aaggtctcct gcgccacttt
    17821 gagtagcgtc accactgcgt gctcgaaaga cggcattacc gtattggcga tgaacacatg
    17881 gttttccacg gtgatcaggc tgttcccact gcggtatcgg ccgggtgggc gaagaacttc
    17941 agaatgtcgg cctcgccggt gtaaatgcca tgtcggtcgc ctgcagccac ggtctggaac
    18001 acactgcggt ataccgccat cggagcgggc gacttcacca tcttgccgtg ttgcagtatg
    18061 gctccatgca gatgcggcag cagaacagtg tcgaacatct catcgggcct agccacgtcc
    18121 agtaccctcg ccgagtcgaa gcgcacaaac aaggcataaa acagcggcag tgtgccggcc
    18181 tgcgcggcgt cggcgtaggc actgcgcagc acccccttgt aactctcggg atagtttttt
    18241 accatggtgg agtcgcccac catttgcttg agcgtaatgg ccagcgcacc gcgctgcaac
    18301 atggcctggg caaaacgctg catggcggca tcgaagccgg gtttgaatgg ttgggccacg
    18361 cgcatctcgg cataaaaggt cacaggacca gaaacggcca actcttctgg ttcggcggcc
    18421 cttgccgacg gtaaaaaacc ggttgaagca agcagggcac cggcactcgt agcgccagcc
    18481 ttgatcatca tggcacgacg ttcgggggaa aaaggaatgg ttggcatgtc atattctcct
    18541 ttgattgggc tgtggcctgg atgaagacgt tgacaagatc aaaaggatgc aggaataaaa
    18601 gggtccccga aaaatcctgt aaccagcgcg cttgcaaatt acaatctgtg cctgatttca
    18661 ggccgcgcat ggatcatcca actgtaatct ggcttcccgg cgtccatttc catcacccct
    18721 aacatgctac agattcaaaa ggttggcttc gaccagccct tttgcatttc tgcgaccatg
    18781 ccagaattgc gtcatgccgt gacacaataa ctcctcccct tgtgccctgg tacggttgat
    18841 tcgcatgagg cgtcagctgc tcggccgaca gatgcgcata ccgccgcagc gtaccaatca
    18901 gcggcatgag ttgtgccggt ttcgttgatt attgcaacaa cacattaccc cccacaccag
    18961 tcatgcatga aacgggtaac gttgctgctg cccctgcagc tgacgattca gcgcagcgcc
    19021 gagaaaatac tgcgagccag cgatccggcg gcctgcatcg ggtgggcgcg gatgttcttt
    19081 tcctcatcag ccatcatgag aaaaaggcca tccatggcct tgttggtgac gtaatcttcc
    19141 agcgtggcat cgttggctgg caccaacccg gtttgtgcgg ccaggccggc aaactggtcg
    19201 tattttttcg tcacaccaac atgggacatg gcctgactga cgataggctt gaagcgtgcc
    19261 gcaattgcgg aaccagtgcg gacacggaag tattgcgttg ccgcatcgtt gccgccggta
    19321 agaatcttgc gtgcgtcatc gatgctcatg gagcgcacgg cgtccaccag cagcgcttcg
    19381 gcctgcggca cggcagcctc ggcggcatgg ttcatcgagg caaccagttg gtcgccatag
    19441 ctgcccatgc ccattttcga caatacatcc tgcgcgcgct gcaaattgcc gggcaacgga
    19501 atgcgcaccc gttgattgtc cagatagccg ttgggctttt ccagcatcga cactgcggtt
    19561 tgtgcccctt gcgtcaacgc ctgacgcaaa cccgtcgact gatccgtagc actgaaacgg
    19621 tcaagcgcgc tgctaccggg atgagctgcc ggcgcactgg tctgcccggg cgccagggca
    19681 ttggcacccg cctgttccaa ctggtgcatc tggtcgggcg tcaccagatt accaagatcg
    19741 aatgcagcag cattgccgct gcacgccaac agcaaccccg ggatgatcca gcggatggtc
    19801 atgttcgctc ccccatgcat aaacagttcg gaatggttct ggtgtagtgc gattgtacac
    19861 cggtagccgc agggaagaac ccttgccgcc tgcgccagcc aatgccggac atgacacacg
    19921 cgcgtcatga tcgcatcgca tactgcgctc atccaccctt gccttcggga gcacgccatg
    19981 ttgcgcctgt tatgcctgtc cggcctgctg tatgtcttgt gtctgcctgc cctcgcagca
    20041 gacgaaccgt ctggcagcgc cccaccggct gccactgtgc tgccgacggg ggtacgcatt
    20101 acgccgcagg ccgcttccgg agcgcagctt gccgaactcg accccggtct gacacggtat
    20161 ccgcatctgc gtgcaggcaa tgcagtcagc agcgcgctga gtccggatgg cagccactgg
    20221 ctggtactga ccagcgggta caaccgctat caggataaga ccggcaagac cgatgctgcg
    20281 gcctcgaacg aatgggtatt tgtctacgat gtccgcaacg atgcgcccca gctgagccag
    20341 gtattgccgg tgcccaatgc cttctgcggc ctggcctgga acccgcgcgg caacgagttc
    20401 tacgtgtctg gcggcgtgga tgatacggtt catcgcttca tgtttgcgaa tggaaaatgg
    20461 gtggagcacc aaccggccat tgcgctgggg cacgcttccg ggctggggat ccatgttcgg
    20521 ccaatggcag cgggcattgc cgtcaacccg gacggcacac gcctgctggt cgccaatctg
    20581 gaaaacgaca gcctgtcgga aatcgatctc gtcgccggca agttgctgcg tgaacgtgat
    20641 ctacggcctg gccgcaacaa cccggggcaa tccggtgtgc caggaggcga atacccctac
    20701 tggccgattt acctttctga ccagcgcgcc gccgtgacca gcctgcggga ccgcgaagtg
    20761 gtgttgctga acacgggtga tacgctgacg gtggctggcc gcattcgggt gggtgggcag
    20821 ccggaacgac tggtcgccag ccatgatggc cgccggctgt atgttgtcaa ctcgggcagc
    20881 gacagcattt cggtcatcga tacgcagcgt acaacggtga tcgacacact gcccacgtcc
    20941 gcaccggcca ccacctttgc caacgcacgg cagctcaccg gcagcaatcc gaacgctctg
    21001 gcgctgtccc cggacgaaca cacgctgtat gtaagcaacg gcggcaccaa tgccatcgct
    21061 gtcgtctcgc tcagcccgcc agctggcgta cgcggactga ttccgaccgg ctggtacccc
    21121 gattcgctgg acatcggccc ggacgggaag cggctgtatg tggtcaatgc caaaggcgaa
    21181 ccgggcgcca atcccggtgg ctgccgcgac acattgtcaa cggcgcacga tgcgcaggac
    21241 agctgccgcg cacgcaacca gtacgtattg caacgcactc attcctcgat cctgaccctg
    21301 ccagtgcctg gcgccgcagc actggctcag ctcacccgtc aggtgtcgcg caacaaccac
    21361 tgggccgatg cacaggacgc acgtgccgtg tgggccgtca tgcgccacgt gcgtacgcgc
    21421 atccatcatg tcatctacgt ggtcaaggaa aaccgcactt acgatcaggt gctgggcgac
    21481 ttgcggccgg gcaacggcga tgcggcactg gtgttgctgc ctgaggcgct cacgcccaac
    21541 caccatgcgc tggctcacga attcgtcacg ctggacaatt ttcttgacag cggttcggtc
    21601 agcggcgacg gctggatgtg gtctaccgcc gctcgcacca gcgactacgc agaaaaggac
    21661 atcccaatac tctatgcagg ccgcgggctg agttatgaca ccgaaggcga gaaccgcaac
    21721 ctcgatctgg ggctggcatc accggcagaa cgccgccgcg agcagccgga cacaccggat
    21781 gacgacgacc tgctccctgg tcatgccgac gtggccgccc cggatgggcc aggcggcgcc
    21841 cccggtgccg gctatctgtg ggatgccgcg ctgcgcgccg gtctgacact acgcaactac
    21901 ggcttcttcg tcaccaatga caatgatggc gaaacggttg cgccttccac ccggccgtat
    21961 gccgaccacc agccacaggc gcacgccaac tcggctgcac tgcgcccata caccgaccgt
    22021 tactaccgtg gattcgatct gaaatacgca gattactggc gctaccgcga atgggcgcgt
    22081 gaattcgccg actacgaacg tcatggcaac ctgcccacgc tcgaactggt gcgcctgcca
    22141 catgaccatt ttggtgagtt ctccgccgca cgcgacggcg tgaacacggt ggaaacgcag
    22201 atgagcgaca acgattatgc actgggcctg ctggtgcagg ctgtcagtcg cagccgctac
    22261 cgcaatgaca cgctgatcgt cgtggtagag gatgatgcgc agaacggcgc ggatcatgtc
    22321 gatgcgcacc gcagcccggc ctacctgatc ggcgcgatga tcaaacggca cacggtggta
    22381 tccacccgct acaccacggt caatctgttg cgcaccatcg aggccgtgct gggattgcag
    22441 ccgatggggc tgaccgatgc gcatgcgttg ccgatgagtg atgcgttcag cgaaaacagc
    22501 cagccgttcg actatcgcgt gcgtgtgccg cagtccctgc acacgacacg gcttcccttg
    22561 ccaccaaccc gtgcggcgag atcggccgta tccgccgctg ccggattgtc acgaccgttg
    22621 catgacagcc actactggag taccgccatg caggggcagg atttcagtgc cgaagaccgg
    22681 ctcgacaccg cgcgcttcaa ccaggcgctg tggcaagggc tcgccccgat agtacggtcg
    22741 acgccacaac acgactgagt acgcggtcgg tcagggctga ccatattgcg gcgaaggcag
    22801 gccaaggtaa tagccctgag cgtaatctac gcccatgtcc tgcagttcgt ccatgatctc
    22861 cgccgattcg acgaactcgg ccacggtgcg gatacccagt tcctgacaca tccgtcccag
    22921 gttgcgtacc agaatgcggt cgacgcgcga attgagcaga ttgcgcacga actcgccatc
    22981 cagcttgacg taatcgaagt gcagttcgcg caggtaatga aatgaattgt agccactgcc
    23041 aaaatcgtcg agagcgaacg aaaacccctt ggcgcgcagg tcggtaagaa attttcgcat
    23101 gttggtcatg tcaccgatcg catcgcgctc cagaatctcg aacaccacgc gttcgggcgg
    23161 aatttcaagt ttctggcaca gcatctcggc aaacccgaga atgccacgcc cctgaatctc
    23221 ttgtgccgac agattgatga acatgcgcgg cggttcactg ccattggtat ccatgtggtg
    23281 gcgcatctgc tgcagggcgt gcgaaatgat cgcccgatcc agatcacgcc ccaatccgta
    23341 tttttccagc gtttcgatga atactccggc cgataccgtc ccgccatcgg gttcaatcag
    23401 acgggccagc acctcatagg caaacattgc cccggtatca caatcgatga tcggctgata
    23461 atagggaatg atctgatcgt tcttgagcgc ctcgcgcaga tactccgcct gatcgcgcat
    23521 gccgcggctg acctgtaacc ggtctttcgc cgagtcgacc atgcccaccc catccttgcc
    23581 accctgcttg gcccgataca ggccgatgtc cactgccgcc atcagatctg ccaccgtgct
    23641 ggcatcgtcc ggataggtca ccacgccgat ggacgtcgtc acgtgaaagc gttttccctg
    23701 tggactgatg aacaccgatt cacgcagctg cacacgcagt ttctccgcca ccaccaggcc
    23761 accttcgcgt gaggtttcgg tcagcaccag ggcaaattcg tccccgccga tacgggtagc
    23821 gacatcaccc ttgcggatca ccgagatgag cagttccgcc acctgcttga gcgcggaatc
    23881 gccgcacacg tggccatagg tatcgttgac atccttgaaa ttgtacagat cgagcatcag
    23941 aagcacgaac tggtgctggt ggcgggccga acggccctcc tcgtagatca ccatctcatt
    24001 gaaataacgg cgattatgca ggccggtcaa cggatcatgc gtggaataat actccaattc
    24061 tgccagcgta cgattgagcg tcttgcttga accgaccacc atcaccatca ccgccagcgt
    24121 ggaccggatt acgctctctt cctgcggcgt caggatatgc gcggaggcga atcccacccc
    24181 aagcaacccg gccagattct gggcttcgag gtccggcacc ggcaccgtga tcaattcggc
    24241 ctgctgcatg tcgatgattt cgctgtagtc agtcacgaca tggtattcct cgatgtccag
    24301 cgacacctcc tcgggcaacc caagcttgac gatcatctca cgcgccagtc gtgtacgcgc
    24361 cacctgcctt acctcggctg gacattcgcc gaggtaatac aggttgagcg acaggccgtg
    24421 cggttcggaa aaggcgatga aaaagaaatt gaacggaaat atggaatgaa aaccctgcag
    24481 aatctcgcgc acgaactgct tccactgggt caccttctca tgcgacagca caatggtttc
    24541 gagcacccgg ctctggcgtt cgagcagatc cttttcaatc agcgtaccgg acagggcggc
    24601 aaccgtgtcg ttgaattcga gcatgacctg tttcaggccg ctttcacttt catcccactg
    24661 gcgctggtac tcacggaaca gcagctgcag ggcttgatcc atgcgcgtcg agcgcgcatt
    24721 gccacggttg aggcactgcc ccagcgagat acgctcggca tcgccaatct gtacctgatc
    24781 gagcagtcgt gaagcctgtt gcaggttccc cgccatttcg gcattgatct cctgcatcag
    24841 ctgacgaaaa ttctgcgcaa actgacgtgt gcgtgtgcgg ttggcgaaca gtgccgcgaa
    24901 acgctgcagc acgcggtcgc gcaatgcttc gatattggcg ctttctaccg gaatcatccc
    24961 tgcgagccca tcctgaatcg cccgctggcg gtcatcagat ccacgccgca ttcgagcttg
    25021 ccgaaccact cgagaaattg gctcaccgcc gccccgcgat acaccggacc atgctgcggg
    25081 gcaatcatgg caatgtccag cgcggatacg gtgtcgaccc atattcttgc ggcgcggttg
    25141 gaacacatgt agcgacgatg aaagctttcg atataaggta aatgtgctgt gaaatcatcc
    25201 acccacaccg ggtccatgtg ccccggcatc tctgccgtgc cgatatcgcc actgaacagg
    25261 atttttgaac gcggatcata aacattgatc tgcccctcgg aatggaggaa gtgcgccggt
    25321 accagctgca gttcaaaacc cggggcgaac tcgcagtgca tgccatcgtc cgccacactg
    25381 atgaaacggt tcagatggcg aatgccataa tgcggcagga aacgggtcca cacccgcgga
    25441 atgtaaatcg gcgcgtcggt cagctcgatc caggtcgaga tcccgccaac aatgtccggg
    25501 tcctggtgcg acaacatgat tgccttcaga ttgtcgggac cgatatgccg caacatttca
    25561 gccaacaccc gcggcatgac atcaaaccca cccggatcga gcagcacacc gctattctca
    25621 tgcaatatca gatactgatt ggaacgaatt ccgtcctctt cgcccgatcc gctttcattg
    25681 agcaggataa accggtgccc cggcgtatcg aacagtggaa tgttacgcac tctgtactcc
    25741 aaacccattg atttgccggg atggtagcat gtgtacgcca gcgaaccaac ggttttaata
    25801 cctcacgatc ttaatttttt ttcactctcc cccaggctta accattctcg tttcgcgcag
    25861 atcacctgac cggatcatat ataagcaact gtgccgatcc gggcacaaat cgcatgtgcc
    25921 cgtcggcgcc ggcggcctgc gccacccgtg cagtcaccag atacagacgt ccggtatcag
    25981 gatccagcgc catggtcctg gccccgggtg ccgtcatgag tgcaggcatg gccgcaaacg
    26041 catgtgcgcc tcgttcggcc accatcgaca gactgccgtc ccggttggaa ctgaacgcaa
    26101 tgccgcggcg cgcatcgaat gcggccgcat cggatccaga cccgatcggc agactggcca
    26161 gcacccgacc attgcgcgca tcaatcgcca gcatgcggtt gttgtggcag gtggcaaaca
    26221 ggtgtgcgtg aactgcatcg atcgccaatc catgcggtgc ctggcaacca gcgatgctcc
    26281 agcgtgcgac gatgcggttg ctggcagtat caatgcgcgc gatctgccgc ttctccacca
    26341 ggttgacgaa taggctgccg tggccatcgg cgactgccga ctccgccggg ctgcccaggg
    26401 tgactgcggc cagcacccgg ttctgcttcg gatcgatggc cgtagccacg ccgctgtcgc
    26461 cgccaatcac gtacacatgc ccactgaccg gatcgtaagc catggcatcg gcatcggcgg
    26521 ccttgagcgc aatcgttttc agcacgcgca gcgtggccag atcgaacact atgacctgcg
    26581 acggatcgct gttatcggcg tagccacggc caagtgccgt cagcaccacc acgccatggc
    26641 cttctccgcc agcatggacc tgtccgatga tggcgccgct gcggccatcg acaaccgtcg
    26701 tttcaccacc atgcgcaatg tacacgcgct tgtcggcagc atcgaaactg aggtaatccc
    26761 accggtcggg cgcgcccagg ggtacggttt tcaccagcgt gtaggccggc gcatccgcct
    26821 gagcctgcgc cggggctgtc cagacagtgg ccgcgccaag tgctgcggcc agcagggttg
    26881 ccatcatacg tattgtcagc atcacgtgtg ctcctcatgc gatagggttt caacggcgcc
    26941 agtttaatct gccgatactt agggttttat gagcgggcca ggatattgcg acctctacat
    27001 gccctgcccc gattgacgaa tgcgcaacgg catcggtaca ttgcgacctg atcgctttca
    27061 tgaatttcgc cagggcattc catgcgcatc tttctgttca cgctaatgct taccgtaacg
    27121 gttgccatct ctgcgccggc atgcagggca gccactcgcc tcgaactggt caaggccgtc
    27181 ctcgtctgcc gccatggcgt acgtgcgccg ggcaagagct cgaacgcgct ggtgcggtta
    27241 tcgccacgcc cctggcctgc atggccggta gctcccggtg tgctgagtgc acacggagcg
    27301 catgacatgc gcttgctggg gacgtttctc ggcacaacct atgcgacaga aggcctgctg
    27361 cccggcacag gttgcaccgc gtcacgccag atcatggtct gggccgacaa cagcacgcag
    27421 cgcacgcggg attcgggaca agccctgctt gacggtctgt tgcctggttg ccaggcccgt
    27481 gcgcatttca gtcagccgat cgaccgacct gacccgatat tccatccagt acagactggc
    27541 acctgtacac tggacgagca cgcagccatg caatccgtgc acgagcatac cggtgatctg
    27601 gcgcagcaga ttgccaggca gcgtccgacc ttgcttgcgc tcggacgact gctgaacccc
    27661 gacatcgaag atgcggcttt gcggcgctgg gcagatgggc cgctgtctct gtcaaccggc
    27721 gcatccggcc acgacctgaa gctgagcgca ccctggggtt cggctgccac ggtgagcgaa
    27781 attttttcac tggaatatgc cgaagggcta cccgtcgatc aggtagcctg gggcgccggc
    27841 gcaaccaccc ctcagctggc gcaattcatg ccattgcgca acctgaaact ggaatggatc
    27901 gaacaggcac cttatatcgc ggcacgtaat ggatcgatgc tggcgcagcg
//
LOCUS       DAIANL010000131        10731 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1600854, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000131 DAIANL010000000
VERSION     DAIANL010000131.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10731
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600854"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1998 a         3105 c         3457 g         2171 t
ORIGIN      
        1 gaatcagtga aattggcgga aataatcgtc atattttgaa atttaaccgt tcgaaacgag
       61 tggaatgcgg ctcaacccgg ttcgttcaga ggtttcctag tcatgtatgc gatcctccgg
      121 cagatttacc gtacaggcat tcgcagcgaa cctgcgccgg catccgatgc cacgctcgat
      181 cgccgtgtgc aggaaatcca ggccgatatc ctgcgcagcg tgggacgtgc gctggcgatc
      241 cgtcatctgg ataccgggtc gtgcaacggc tgcgaactcg aaatcggtgc gctcggcaat
      301 ccgtattaca acatcgaggg tctcggagcc cattttgtcg ccagtccgcg ccatgcggat
      361 ttgttgttgg tgaccggtcc gttgtcacgc cacatgctca cggcgatgcg acgcacctgg
      421 gaggccatgc ccgaaccgcg tctggtggta gccgtcggcg attgcgcgaa ggacggcggc
      481 gtgtttggcg aaagttacgc ctgttgcggc ggcgtgctga accaccttcc ggttgatgtg
      541 ctggttccgg ggtgtccgcc gtcaccgctg gcgattttgc aaggcattct gagtgcggtg
      601 gcgggacgcg gcgagacgcc agtgcagtca caatcgatat ccggccagac ggcagaaaat
      661 ctgggataat taccgattgt tgacaggaaa tggaaatggt gcggacatgc aaaccctgac
      721 cgtcgcattg ggccatcgca gttatccgat tcacattggt gccgggttgc tggatcagcc
      781 cgaactgatc gcggcgcatc tgccacagcc gcgtgcggcg atcgtcacca atacgaccgt
      841 ggccccgctg tatctggcgc ggcttgcggc cggtttgcgc gcccatggcg tcgatgtggt
      901 ggagatcgtg ttgccggacg gggagcaatg gaaggattgg gcgacactga accagatttt
      961 cgacagcctg cttgcacacc attgcgaacg caaaacgacg ctggtcgcgc tcggcggcgg
     1021 cgtggtgggc gatctgaccg ggtttgccgc agcctgctac ctgcgcggcg tgccattcat
     1081 tcaggtgccg accacattgc tggcgcaggt ggattcctcg gtcggcggca agacgggcat
     1141 caaccacccg atgggcaaga acatgatcgg tgcgttctat cagccacgcc tggtgctggc
     1201 cgatacggat acgctgcata cgctgccggc gcgggaactg tctgccggcc tggccgaggt
     1261 gatcaagtac ggactgatcc gcgatgccgg gtttttcggc tggctggaga cgaacatgga
     1321 acggttgcgt gcgctcgatg agcaggcgct ggtgcatgcg attcaccact cgtgcgcagt
     1381 caaggccgag atcgtggcgg gcgatgagcg cgaatccggc atgcgcgcct tgctgaacct
     1441 gggacatact ttcggtcatg ccatcgaatc cgccatgggc tatggcaact ggctgcacgg
     1501 ggaggcggtg gcggcgggca cggtcatggc ggcacggctg tcgcagcgac ttggctggat
     1561 cggtgaaggc gatgcggcgc gcatcgcagc catcatgcgc agtgccgggt tgccagaccg
     1621 gccgccggag ctggacgtcg aggtgtggat gcgcggcatg cagcttgaca agaaagtgga
     1681 agacggtcgc atccgcttcg ttttgctgcg cgcgatcggg gagtcctgtc tgagcgcgga
     1741 cgttccggtc gaattgcttg aacgggtgct ggcggacagc gcgggctgag accgatgcca
     1801 ggggtgcgca gccggagcag gatgcgccag accgattttt accggatttc tcagaaattc
     1861 gtgtcaaccc gttctattat ttgttatatt tccgtcatac attacctggg cgatcaccat
     1921 gaaaccgatt tccgctgctt tggcgttgct ggtctgcgta gccttgcctg ctcaggcgca
     1981 gaatctgcca caggcgggcg cgccgatcat tgccacgccg gtcgccgtgc ccgccagtgc
     2041 catgatgacc ggggcggcca ccagtgcttc ggtaacggcc cccgtggaaa caccggcagc
     2101 gccagcggct caggcggcca cagaagctgt gcctgtgcag agcatgctgc cggcagccgc
     2161 cccggcgccc gcacctgcac ctgcaaccgc agctgtgccg acgcctgccg cagtgcaagc
     2221 cgcacctgca gcggcagcgg ccgaagcgcc ggctgcgtcc gaagtgcctg caagcaatgc
     2281 gccacgcatg accgccggca tgccgcgcct gagttcgcac tccgtgctgg tgatcaatga
     2341 ggccaatggt gcgccgttgc tggaaaagaa tcccgacatg cagacgccga tcgcatcgat
     2401 cagcaagctc atgaccgcga tggtggtgct ggatgcaaaa caggacccca ccgaaaaaat
     2461 cgaagtgaca gaggcagatg tcgataccct gcgtcactcc gcctcgcgtc tggccgttgg
     2521 aaccgtgctg acgcgtgacc agatgctgca tctggcgctc attgcttccg aaaatcgcgc
     2581 tgcgtcggca ctgtcgcgca attatccggg tggacgggtg caattcatcc gcgccatgaa
     2641 tcgtaaggcg gcccagcttg gcatgacgca tacgtcgttc gaggatccga ccgggctggc
     2701 gagccacaac cgctcgacgg ccgaggatct ggcgaagatg gtcaaggcgg cagcgagcta
     2761 tccgctgatc caccgcatca cgaccaccgg tgaatacgat atccggcgta cggccagtgt
     2821 ggaggtgcac cgcaggcacc ggcacgcggc agcgcgccat ctcgtgaccc gggagattgc
     2881 cttccacaac accaaccagc tggtgcgcga gaatcggttg gacatcggct tgtcgaaaac
     2941 cggtttcatc aatgaagccg gccattgcct ggtcatgcag gtgcgtattg cgagtcggcc
     3001 ggtgatcatc gtgctgctcg acgcctatgg caagtttgac cgcatcagcg atgcgcgccg
     3061 catccatcag tggctggaag cccacagcag cgtttttggc aaggaagcgc gtcgcgctcg
     3121 cgccggaaac cggctggcaa gtaatccgta acggtttgcg gcccggggtg ggcaggagtg
     3181 ttcgtgctgg tctacatcca cggctttaac agcagcgggc gctctgccaa gggacgcctg
     3241 ctggtcgact atttcgccgc cgcagatcgg ttggacgagg tgtggtgtcc ggatctgccg
     3301 caccgtccgg cgcaggcgat ggccatgctg gaagagcgac tggcgcatca tgcccctgcg
     3361 gacatacggc tggtcggcag ttcgctgggt ggtttttatg ccacggtgct ggccgagcgg
     3421 atgggcgtgc gcgccgcgct cgtcaacccc gcggtgcatc cggcccggtt gctggaggcc
     3481 gcgctgggcg aacagcaaaa ctatcacagc ggagaacgtt atgcgttcac ccgcgatcat
     3541 ctgcgcgaac tggcagcact ggagccgtcg ccgctgcaac atccggaacg attgctgctg
     3601 ctggtggaaa ccggcgatga agtgctcgat tatcgcgagg ccttggcgta ttatgacgcc
     3661 tgccgtcaga ttgtggttcc tggcggcgat cacggtttcc ggtcttttgc ccggtatatt
     3721 ccagagatta tgacgttctg atcccgcccg ccgggggcct gcatgtacct gttatacgaa
     3781 gaagaaggcc agttcaaggc aggcaccata gtcgccgaca acgatacttc gctgcaaatc
     3841 gatacccagt ttggcaagcg catcaagttg cgtgcagccc aggtgatgct gcgattcaca
     3901 gcgcctgtgc cggaggcatt catgcaggcg gcaggcgagg tgcgcgcagc gcttgatccc
     3961 gattttctgt gggaagtcgc cggtcaggaa gagttcgact acactgtgct cgcccaggag
     4021 tatttcggtc gcacgccgca gcccgcagag caggcgggca tcctgcagtg tctgcatgat
     4081 gcgccgatgt atttctaccg caaggggcgc gggcgttacc gtccggcgcc gcccgaggcg
     4141 ctgactgcgg ccaaggccag tcttgtgcgc aagcagcgcg aggcggaact ggtggcagag
     4201 tatgtcgccg aactgtcggc attccgtctg ccgccggcaa tgcgtgacga ggtcgattcg
     4261 ctgctgtatg cgccagacaa gcagcaaccg gcctggcgcg ccgtcgaggc tgtgtgcaaa
     4321 cagaccgggt tgacgccgct gcgcctgctt gaccgttgtg gtgccatcgc atccagtcat
     4381 gattaccacc tgaaactgtt cctgcgcgag tatttcccgc gtggaaccgg gtttgccgtg
     4441 gacgagacct tgcctgcatt gcctgatctg ccgctggcca cggtgcgtgc gttcagcatc
     4501 gacgatgccc agaccaccga gatcgacgat gcattttcgg tgacgcgcct ggccgatggc
     4561 gactggcaag tgggtattca catcgcggcg ccggcgctgg gggtgctgcc aggcactgcg
     4621 ctcgaccagg cggccgcggc acgcatgtcg acggtctacc atcccgccgg caagatcacc
     4681 atgctgccgg aagcggtgat cgcagcgttc tcgctggatg agggacgtga ttcgcctgtg
     4741 ttgtccatgt acctgcaggt ggccgatggc agctgggagg tgcggtcgca caccacgcgg
     4801 ctggaatgcg tgagggtggt ggagaatctg cggcacgaaa cattacaggc gcattttgat
     4861 ccggaatgcc tggtgaacgg ctgcccgcaa tatccatggc aggacgagct ggccatgctg
     4921 tggcagttgt cgggcaagct tgaagctgcc cgcggcaaga cacccgatcc gtcccagcct
     4981 cagcgcgtgg attaccagtt ccgcgtcgat ggtgaacgcg tgaccatcac cgatcgtcag
     5041 cgaggcaacc cgatcgatcg tgtggtatcg gagctgatga ttctggtcaa tgcggaatgg
     5101 ggcaggcaac tggcgcaggc gggtgccatg gcgctgtacc gcacccagca gaacggcaag
     5161 gtcagaatga gcagccggcc agcaccgcat gtggggctcg gcgtcgagca gtatgcctgg
     5221 gcaagttctc cgttgcggcg ttatgtcgat ctgctcaacc agcgccagct cattgcgctg
     5281 gtgcaggacg agccgccgcc ttatccgccc aaatcagata tactgtatgc cgcattgcgc
     5341 gatttcgaaa cggcgtatga cgcctactcg gacttccagc gcaagatgga gcgctactgg
     5401 tgcctgcgct ggctgctgca ggagcaggaa acactggtgc atgcggtggt gatcaaggaa
     5461 aacctgctgc gcctcgacaa cctgccgatg gtggttcgtg tgccctccct gccggaaacc
     5521 ctgccgggta ccgatgtggc gctgcgcgtg gatggcgttg atctgctgga agtcgaactg
     5581 cattgtacct ggttgccaca ggagtgatga gtattgaatc cggttcgcga aagagacgaa
     5641 tgcctggagg cggcggactc cgggaattcg ggtacagacc tgcctgctgc ggttgctccc
     5701 gatgccgctg agtgagcgag cgcttcgaac catgtttttc ccgcgtgcaa tgcccggcgc
     5761 ctttcgcctg gccttgctgc tgtcgttcat cctgcacacg ctgctgctgg cggtgaggtt
     5821 ttccctgccg gatattcgtg cgcaatcttt catgtcgccg cagctcgaac tggtgctggt
     5881 caacagcaag tcggtcactc cgccggagaa ggctgatgcg ctggcgcaga gcaatctcga
     5941 tggcggcggc aataccgacg aagaccgtcg ggcaaagagt ccgctccctg caatgtcaca
     6001 agaccagcgc cagcaggata tcgaggccgc ccagcgtcag gtcgcgcggc tcgaagaaga
     6061 aaaccggcag ttgatgaccc agatccgtgc ggcggtgcag ttgcccgaca aacccagcac
     6121 cacggaacag aaccgtgccc cgctggccga ccattccggc gatggtcagg gaaaggcgag
     6181 ttcgctggaa atgtccgagc tcgaagcgca gatttcgcag caatacgatg cctaccagaa
     6241 acgtccgcgc aagctgttca ttggtgcgcg tacccaggaa tatgcttttg cgcgctatgt
     6301 ggaagactgg cgcatcaagg tcgagcgcat cggaaacgaa aattatccgg cagcttcgcg
     6361 cgacaacaaa atctatggca agttacagct gaccgtctcg attcgtgcgg acggcacact
     6421 ggacagtatc gaggtgacgc gttcttcggg ttcggcggtg ctggataacg cggccattgc
     6481 catcgtcaat cgtgcggcgc cattttcgcc atttcccgag gatatacgca aaaaggcgga
     6541 cattgtcagc atcacgcgca catggacttt cgcacccagc gaccagctga gcactcatga
     6601 ctgactggcg ccagccggtt ggttgttgcg cgcttctgtt ataatcgcaa cattccctat
     6661 tctgaaaaag gcgacatcat gcagattggc accaccttga cccagttcct tgccgacgaa
     6721 cagcgccgcg caggcgtgat cactgatgat ttgcgtattc tgctgggcga ggtgtttacc
     6781 gcgtgcaagg tgatctccaa tgcgatcaat cagggggcgc tggccggcgt gatgggcaat
     6841 ctggattcgg agaatgtgca gggcgaaacg cagaagcagc ttgacgtgat caccaacgaa
     6901 attttcatcg aggcttgcga acgcagtggt cagctggccg gcatggcatc cgaggaaatg
     6961 gaagacgtct accgcattcc ggacaatttc gtgcgtggtc gttacctgct cgtgtttgac
     7021 ccgctcgatg gatcgtccaa tgtcaacgtc aacatttcgg tcggcaccat tttttccgtg
     7081 ctgcatcatg atggggatgc atcgcatccg gccgtcagcg attttctgca gccgggcacg
     7141 cgccaggtgg cagcgggtta cgcgctgtac ggttcttcga ccatgctggt gctgaccacc
     7201 ggccagggcg tgaatggatt tacactggat cgcaacatcg gcgagtttgt gctgacccac
     7261 ccgggcatga ccattcctgc ggacaccaag gagtttgcca tcaacgcttc caaccagcgt
     7321 ttctgggaag cgccggtgca acgttatgtt gccgaatgcg tggcaggcaa gaccggtccg
     7381 cgcgggcgtg acttcaatat gcgctgggtg gcttccatgg tggcagaggt gcaccgcata
     7441 ttggtgcgcg gtggcgtttt catgtatccc aaggacacca aggatccgac caaggcgggc
     7501 aagctgcgcc tgatgtacga ggccaatccg atgagcttca tcgtcgagca ggcaggcggg
     7561 atgtccagta cgggtcgtca gcgcattctt gacatccagc cggaagggtt gcaccagcgc
     7621 gtgccggtca ttctgggttc aaagaacgaa gttgaactcg tggtgtcgta ccaccagggt
     7681 cattgatacc gcttcacagc ggcggcgttc gggtctggcg ccgccgcaac gcatgatgtc
     7741 agtctgctcc aggtcgccaa tgcagtagca gtccgggctc acccgggcgg gcctgccagg
     7801 cgcagtcggt gccgataccg accacgtgcg cgagctggtc tgaacaccag acgaaaggca
     7861 ggtgcgcgcg ctgccacgac ggtatgcctg attcctgtaa ccatttcttc accgcacggc
     7921 gtggtcggcg gcaatacggt tgcaggtttt cgccaccgat gcgggcacga atgatcattt
     7981 cgtctgcatt cggcaggcgc attccggcgc cggtcaccat ttcaaattcc agtctgccgc
     8041 ccaattcggg cagttcgagt tcgggctcgc cgtgccagcg ccagcagatg ccgggcggcg
     8101 gcatctgttg ttcccggacc agccaggccg agccgtggct ggcgtgcagg acgagggtgc
     8161 ctaactggat gcgcagacgt gtgtcatggc ttgcctgaac cagttggcgg tacatttcgt
     8221 gcagcaatgc gctatcgggc atatcaagcg cgtggccggc gagaaagtgg cgtagcaggt
     8281 tacgcgcacg tgcggcgctg agtctggcaa gcgcgccaca atccagccgc cgttcatgga
     8341 tagccgttgc agcgtcctgg ttggccagat cttcgagcag ttcggttgcg tcggccatgt
     8401 ggctggcggt gcgcgccagc gtggtgcgcc aggcgggggc caatgcttca atcgcgggca
     8461 ggacatgctg gcgcaggtgg ttgcgcgtgt aacgcgtgtc ggcattgctc tcatcttcca
     8521 cccaatgcag cccgcgcgat tcggcccagg cccgtagccg ggcgcgctca atgccgagca
     8581 acgggcgcaa cagcatcgga ccatcttcca gccggcgcag tggtggcatg gcggccagcc
     8641 cctgcacgcc agcgccgcgc agcagctgca atagcacggt ctcggcctga tcgtcacggt
     8701 gatgcgcaag cgcaaggtaa tcgacagggg tgcgacgaaa gcactcgtat ctcaggctgc
     8761 gcgctgccgc ttcgagaccg tgccggtcat ggcggtcgac gtgtacctcc tggatgtgca
     8821 acgggatgtc gagccgttcg cacagccggg cgcagaaaca ggcccagtcg ctggcgtgag
     8881 ggctgagccc gtgatgcaca tgcacagcag atagcggatt gcggtcacgc atgccggcca
     8941 gcaggtgtag cagcacgaca gaatccatgc caccactcag ggcgacaccg atgcgtgcgc
     9001 ccggcgcaac ctgtgccgcg cagtagcggg caaccgtttc aggcagcgct tctggcggag
     9061 ttgtctttgt agcggccatg actcatcagg cgctgatagc gggctttgag cagggcatcg
     9121 agaggttgct tgcagatttc cgccagtgtt tcggtcaggg cggctttcat gttgtgcatc
     9181 atggttggcg ggtcacggtg tgcaccgcca gttggctcgg ggataatgcg gtcgatgagg
     9241 tcaaatgact tgagtttgct ggcggtgatg ttcatggttt cggccgcggt gggggccatt
     9301 tcggcatttt tccacaaaat cgatgcacag ccttcaggag aaatgaccga ataggtcgag
     9361 tattgcagca tcagtgtgcg gtcgcctacg ccgatggcaa gagcgccgcc cgaaccacct
     9421 tcgccgatga cagtgcacag gataggggta cgcagttcgg ccatgacgta gagattgcgg
     9481 gcgatggctt ccgactggcc gcgttcctcg gcaccgatgc caggatgggc gcctggcgta
     9541 tcgatgaagg tcatgatcgg gataccgaat ttttctgcca ggcgcatcag gcgcattgcc
     9601 ttgcgataac cttcgggtcg cggcatgccg aagttgcgca ggattttttc cttggtgtcg
     9661 cggcctttct ggtggccaat gaccatgacc gtctggtcgt tgaaatgacc cagaccgccg
     9721 acgatggccg gatcgtcggc aaaggcccgg tcaccgtgca gttcctcgaa atcggaaaat
     9781 agcgcgccga tatagtccag gctatacggc cgttgcgggt ggcgcgcaac ctgcgagatt
     9841 tgccacggcg tcagttttgc atagatgtcc tgggtcagct tttcgctttt tttctgcagc
     9901 agattgagtt cgcgtgaaat gtcgacggcg gaatcgtcct gcatgttgcg cagttcctcg
     9961 attttccgtt cgagttcctc gatgggctgt tcaaattcga gaaaggtggt tttcattgga
    10021 atgatgcctg gtccggtgtg caagtaagct gcatgggcaa gcggcgagtc gcgcaggctc
    10081 agtgttcgtg gccgtgctcg ccatgggcgt gaccgtgcgc aatttcgtcg gcagtggccg
    10141 ggcgcacgtc gatcacggtg cagtcgaaac gcagggtctg ccccgccagc gggtggttgc
    10201 cgtccaccac caccgtgtct tcggtcaagt cggtgaccgt atagagcatg tagtcgtcgc
    10261 taccttcggc tgccccctcg aattgcatgc cgaccacggt ttcggcggga aacaggttgc
    10321 gcgcctcgat gcgtaccagt tcggcttcgt attcgccaaa cgcatattcg ggctccatgg
    10381 tcagttcgac ccggtcgccg acctgtttgc cctccagggc ttcctcgacg gtagggaaga
    10441 tgccgtcata gccgccgtgc aggtaactga caggctcctg cgcgtcgacc tgttccagga
    10501 gttcgccgga caggttgctc agttggtagg tgatggtgac gatggtattc ttggcaattt
    10561 gcatgacaat gcccgcctgc acgtttaagg aacctctgaa cgaaccgggt tgagccgcat
    10621 tccactcgtt tcgaacggtt aaatttcaaa atatgacgat tatttccgcc aatttcactg
    10681 attcaaggtc attttcgacc ctgaacaccc cgtttccggg ttcagtgggt g
//
LOCUS       DAIANL010000132         4365 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3232479, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000132 DAIANL010000000
VERSION     DAIANL010000132.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3232479"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          935 a         1363 c         1184 g          883 t
ORIGIN      
        1 gccggagcga tgacctccat gctcgataca cggtaattgg gcaggaacgc gaccttgaga
       61 cgaccatcga tgtccggatc gctgttcacc acttcggcga tattgttgat cagcttgatg
      121 atgcgcttgg ccatgcggta accgggagcc gccttgcccc ccaccagcac gcagcggttg
      181 accagacgtt ccatgtggcc gcgcttgatg cgcaggtaca ggtagacgat gtgcagcgca
      241 ttgagcaact ggcgcttgta ctcgtggatg cgtttgacct gcacgtcaaa cagcgcattc
      301 gggtcgaaat cgacgttaca ttcggccttg accatggctg ccagccgccg cttgttttcc
      361 agtctgactt cacgccagcg cgcgtgaaac gtctcgtgct gcgtctccgc aaacgggttg
      421 agcgaggcga tgcgggagaa atccgcgacc cattcgtcgc caatgtgctc ggaaatgagc
      481 gcggtcagtc ccgggttggc atgcgccacc cagcggcggg gagtgacacc attggtcttg
      541 ttgttgaatt tttccggcca catcaggaaa aaatcatgga acaacccctc gcgcagcagg
      601 cgcgaatgca gttcggcaac accattgacc gaacagctgc ccacgatcgc caaccaagcc
      661 atacgcacct gacggacagg cccttcctca atgatcgaca tacgccgcaa acgctcggta
      721 tcccccggcc atttcatgga cacctggcgc agaaagcgcg cattgatttc gtaaatgatt
      781 tccagcaagc gcggcaacag gcgctcaaac aggctcacag gccatttttc cagcgcctct
      841 ggcaacagcg tatggttggt ataggccacg cagcgcgtca ccacttgcca ggcatcatcc
      901 cagcctacgt cgcgctcgtc gagcaaaatc cgcatcagct cggcaactgc gatcgtcggg
      961 tgggtgtcgt tcatctggaa caggttttcg tcggcaaaac actgcagatc gaactcggtg
     1021 tgacgcatcg acacacggat cgcgtccttg atgcttgccg aggcgagaaa atattgctgg
     1081 cgcaggcgca attccttacc gttttcgctg gcgtcattgg gatagagcac cattgatatg
     1141 tcttctgcct gattcttcga ctgcactgcc tcactgtagc tgccggcgtt gaattccgcg
     1201 agattgaatt catcggtcgc cgccgccttc cacaaccgca acgtattgac cgtgtggttg
     1261 cgatagccgg aaatcggcat gtcgaacggg atcgccacca catcgttggt gttgatccag
     1321 cgtacgcgtg tacgtccatg atcatcgaca tactgctcgc tgcgtccgcc aaactgcacg
     1381 atctgactgt attcgatacg ctccacttcc cacggattgc cattctgcag ccaaaggtcg
     1441 gccgcttcct tctggaaacc gttttcgatg tactggcgaa acatgccata ttcataacgg
     1501 atgccatatc ccatcaccgg cagccgcagc gtggcgcaac tgtccatgaa acaggctgcc
     1561 agccgaccca gtccgccgtt gccaagcccg gcatcaggtt catcttccac cacttcttcc
     1621 aggcgttcgc tgtagtcaat catggcctga tgtgtcgcgc cttcgaggtc catgttgagc
     1681 agatggttgc ccagcgcgcg accgataaga aattccatcg acaggtagta catgcggcgc
     1741 acacccggcg cccactgctc gttccaggta ttgcgccagt ccgccatgag ccggtcacgc
     1801 acggtatagg acaacgcctc gtaaatgtag atcgaggcgc aaccgcgcac acgccccagc
     1861 cgatacgaca ggtaatgctt gaaatcccgg ccgagcgact cggcatcggc cggcaatgcc
     1921 tccatccggt aaggacgata ggtctgcgga acgtattttc cggttttcat gccaatccct
     1981 tcattcattc atcatgaccg gctgaccatg gtacagcgca agataggtca tggcgctgtg
     2041 ttcccagcca aaagactggt tcatgccggt acggaccagc gcgtgccagc gccggggttg
     2101 cgcataacag gccagggcgc ggtccagcgc atcactcagg ccatgcggac tcagttcgtc
     2161 aaatacaaat ccgttggcaa tgccctcgtc gacatgcccg tcgctggcat cgaacacggt
     2221 atcggccagt ccgccggtgc gatacacgat cggcggtgtg ccatagcgca gactgtacat
     2281 ctggttcagg ccgcacggtt caaaacgtga cggcatgaga aacatgtcgg ctcctgcctc
     2341 caccaggtgc gccagcattt catcgtagcc aatgtacaca cccagccgtt ggggatgcag
     2401 tgcagcgagt tcgcgcaact gttgctcgaa atgggcctgg ccgctaccga cgataacgaa
     2461 acgcgcctgg gtgcgcgtga accagtcggg caatgccgcc agcaccagat cgatcccctt
     2521 ctgttccacc agccgtccga ccatgccgac cagtggcacc tgctgtgcgc tttcgccccc
     2581 atgcatatcg aattgccgca gcagggcctg cttgttcaac gccttgcctg gttgccgccg
     2641 ccgcgccgaa tagtgtgccg acaggaacac atcggtttcc ggatcccaca acgtctcgtc
     2701 gataccgttc agtatgccgt gcaatttatg acgcgagacc tgcagcactc cctgcaaccc
     2761 gtaacccgca tccgatgtgc aaatttcatg cgcataggtc ggactgactg tggtcacttc
     2821 atcggcatac acgatgcccg ccttgagcat ggaaaaacca ccgtagaatt ccacgctatc
     2881 gaccgcccac caatgccggg gcaaaccgag gcgatagaac tgatcggggc caaaatggcc
     2941 accgaacgac agattgtgaa tggtaaacac cgacttcggc cgctcaggaa gctctgacag
     3001 cagcgctggc accagtccag tctgccagtc attggcatgc accacatcgg cgcgccaccc
     3061 cagttcgagc gcatcggtgg ccagtgccac cgtcacccgc gccagcacgg taaaccgctc
     3121 ggcgttgtcg ggccagtcat ggccgtcagg ttgcatgtaa gggttaccgg gacgatcata
     3181 caattccgcg cagtcaacaa gccagagcgg aaaagcgaaa ccctcctgct gcacttcaag
     3241 aatgcgcacg ttgtgcatcc tgcctgcacc ctggacctcg gtccaggcga ggacgcgcat
     3301 ggcctgtgcc tgcgccagcg cgacgcggta ggcaggcatg accacgcgca cttccgcacc
     3361 gacacggtgc aacgcatgcg gcaaactgta gccaacatct gccagaccgc ccgtcttgac
     3421 caaagggtaa atttcgctga cagaaaacag cacccttgtc tttccatgca tgacgcttat
     3481 tccatattca atttgagaaa aacggcggcg agcggcggca gggtcaatgt aagcgaatgc
     3541 gcatacccca tccacgctgt ttcctgactg tgcagcaggc cggcgttgcc gaggttgctg
     3601 cccccataat aagccgaatc ggtgttgagc agctcgctgt accaaccgcc ctgcgggacg
     3661 ccgatccggt attcctgacg tgggaccgga gtgaaattga gcgccacgac aatgaattcc
     3721 ccttcgctct gacgcaccag gctcagcacc gactggtcgc tgtcgtggca atctatccag
     3781 tgaaagccgc gactttcaaa atcgtacatg tacagtgcag gctgattgcg atacagcgcg
     3841 ttgaggtcgc gtaccaggtg catcaccccg tcatgcaccg gaaagccgca caacgtccag
     3901 tcaagcgcgg actgcacgtt ccattcgttc cactgggcga attccccgcc catgaacagc
     3961 agtttcttgc cgggatgggc atactgccag gcgtagaaca ggcgcagatt ggcgaaacgc
     4021 tgccagtaat ccccgggcat cttgtcgagc atgctgcctt tcatgtgcac cacttcatcg
     4081 tgcgacagcg gcagaacgaa gttttccgta taggcgtaca gctggccgaa tgtgagctgg
     4141 ctgtgatggt acttgcggtg gaccggatct tcctcgatat atgccagatt gtcattcatc
     4201 cagcccatgt tccatttcat ggaaaaaccc agtccgccca gttccaccgg ccgcgacacc
     4261 atcggccagg cggtcgattc ctcggcgatg gtcagtaccc ctggcaaccg gccgtggacc
     4321 acactgttga gttcgcgcag gaattcgatc gcttcaaggt tttcg
//
LOCUS       DAIANL010000133         9444 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3232527, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000133 DAIANL010000000
VERSION     DAIANL010000133.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3232527"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1787 a         2655 c         3189 g         1813 t
ORIGIN      
        1 ggaaacgctt gaccgcaatt gtgccgattt cggtatcgtc gaatttggca tgaacgatcc
       61 gcgtcagggc atcgtgcacg tcatcggccc agaacagggc tataccctgc ccggcatgac
      121 ggtggtgtgc ggcgattccc acaccagcac ccacggtgca tttggcgcgc tggcgttcgg
      181 cattggcacc tccgaggtcg agcatgtgct ggcgacgcaa tgtctggcca cgcacaagtc
      241 gaaagcgatg cgtgtctggg tggaagggca gctcggcgca ggcgtcacgg ccaaggacat
      301 ggcgctggcc atcattggtc acatcggtac cgcaggtggt actgggcatg ccattgagtt
      361 ttccgggcct gcggtgcgcg cgctgtccat ggaaggacgc atgaccctgt gcaacatggc
      421 gatcgaggca ggagcgcgtg ccgggatggt ggcggttgac gacactacgc tcgactatgt
      481 gcgtggtcgg ccgcatgccc cgcagggcga catgtggcaa caggcgcagc agtggtggcg
      541 aacgctgatg tccgacgagg atgcggtatt cgatcgcgaa gtgcggctcg atgcggccgg
      601 catcctgccg caggtgacct ggggcacgtc gccggagatg gtggtgccgg tgactgctgc
      661 ggtgccggat ccggacgtga tgaccgatcc ggtcaaggca gccgatgcgc gtcgtgcgct
      721 ggcctacatg ggattgcgcg ccggtgagcc gatggccgag gtcgcagtcg acaaggtgtt
      781 catcggttca tgcaccaact cgcgcatcga agacctgcgt gcggccgctg cagtcgtacg
      841 tggccggcac aaggcggatt ctgtgaagca ggcgctggtg gtgccgggtt cggggttgat
      901 caagcgtcag gccgaggccg aaggactgga ccgcgtgttc atcgccgcag gcttcgagtg
      961 gcgcgatcca ggctgctcga tgtgcctggc gatgaatgcc gaccggcttg agccgggtga
     1021 acgttgcgcg gcaacctcca atcgcaattt cgagggccgg caggggcagg gcgggcgcac
     1081 tcatctggtc agcccggcca tggcggcggc ggccgcggtg gccggacatt ttgtcgacgt
     1141 gcgtacctgg caggagaacc aatgatgcaa cccttcacaa cgctggatgg gcttgtgctg
     1201 ccgatggatc gggccaatgt ggacaccgat gccatcatcc ccaagcagtt tctcaagtcg
     1261 atccgccgca gcggttttgg tgtgaacctg ttcgacgagt ggcgctatct ggatcatggc
     1321 gaacctggtc aggagtgcag cactcgcccg atcaacccgg atttcgtgct caatcagccg
     1381 cgatatcgcg gcgcggaaat cctcctggcg cgtgagaatt tcggatgtgg ctccagtcgc
     1441 gaacatgcgc cctgggcgtt ggcggactac ggactgcgtg cgttgatcgc gccgagttat
     1501 gcggatattt tttacaacaa ctgtttcaag aacggactgc tgcctatcgt actcgatgcc
     1561 ggtacggtgg accggttgtt ccgcgaagtt gcggccacac cgggataccg tctgcgcatc
     1621 gacctggctg cacagacggt gacattgcct ggcggtgata cggtgcgctt cgaggtggat
     1681 gcccatcgca ggatgtgcct gctcgaaggg ttggacgata ttggcctgac cttgcgccat
     1741 gtcgaccgaa tccgtgatta tgaagctgcg cgccgtcagt cggcgccgtg gctgttcgaa
     1801 cgttcgtgaa ccttaaaccg aggacgagaa gatgaagatt gctgtgctgc caggagacgg
     1861 gatcgggccg gaagtgatgg atcaggcggt caaggtgctg catgcgctgg tgtccgacgg
     1921 gctggcgctg gagatggaat tcgcgccgat cggtggcgcc ggctatgatg cggctggtga
     1981 tccgttgccg gcggcgacgc tcgaccttgc acgccaggcc gatgctgtgc tgctgggtgc
     2041 ggttggcggc catcgctatg acagcctgcc acggccgatg cgcccggagc aggggctgct
     2101 gcgcatccgc aaggaactca atctgttcgc caatctgcga cctgcagtac tgtatcccga
     2161 actggccggt gcatctacgc tgcgtccgga agtggtatcc ggtcttgacc tgatgatcgt
     2221 gcgcgagctg actggcgacc tgtattttgg ccagccgcgc ggcatacaca tcaacgcggc
     2281 cggcgaacgt gaaggctata acaccatgcg ctattccgaa tcggaaatcc gtcgggtcgc
     2341 gcacagcggt ttccgcatcg ccatgaaacg tggccgcaag ctgtgttcgg tggaaaaggc
     2401 caacgtgctc gagaccagcg aattgtggcg tgaagtggtg gtcgaagtgg gtcgcgagta
     2461 cccagaagtc gcgctgtccc acatgtacgt ggacaacgca gccatgcagc tggttcgcaa
     2521 tccgaagcag tttgatgtca tgctcaccgg caacattttc ggcgacattc tctccgacga
     2581 ggcgtccatg ctgaccggtt cgatcggcat gcttggttcg gcttcgctcg acgaacacaa
     2641 caagggcatg tatgagccga ttcacggttc ggcccctgac atcgctggac aggacattgc
     2701 caatccgctg gccaccatcc tgtctgcggc catgatgctg cgttatacgt tctcgcttga
     2761 cgaggcggct gaccgcatcg aaaccgctgt caggcgagtg attgcacagg gattgcgcac
     2821 gctcgatatc tatacgccgg ataccacccg ggtggggtgt gcggcaatgg gcgatgcggt
     2881 cgtggcggca ttgtaggcac aggcaggatc aggggtagaa aatgaagatg aaagcgggtc
     2941 tggttggctg gcgtggcatg gtgggttcgg tgctcatgca gcgcatgcgt gaagaacgtg
     3001 atttcgaaca tttcgaaccg gtgtttttca gcacctcgca acggggcggt gtggcgcccg
     3061 atatcgggcg cccggtggcc gcgctgcagg atgcccgcga tatccaggca ctggcgcaga
     3121 tggatgtcat cgtcagcgcc caggggggcg actacactac cgaaatgcac ccaaaattgc
     3181 gtgccgccgg ctggcagggc tactggattg atgctgcctc cacgctgcgc atggcagaac
     3241 acagcgtcat cattctcgat ccggtcaacc gcacggtgat cgatgaggca ctgcatgccg
     3301 gcatgcgcga tttcatcggc ggcaactgta ccgtttcact gatgctgatg gcgctgggag
     3361 gtctgtttgc tgccgacgaa gtcgagtggg tgacctcgat gacctaccag gcagcgtctg
     3421 gcgccggtgc gcaaaacatg cgcgaactgc tcgcccagat gggcgcggcg cacgatgcgg
     3481 tggcaccgct gcttgccgat ccggcatcga tgattcttga cattgatcgc acgctggcca
     3541 ccacgttgcg cgacaccagt tttcccacga cccagttcgg cgtcccgctt gccggcagcc
     3601 tgattccatg gatcgacaag gatctgggca atggccaaag tcgtgaggaa tggaaaggca
     3661 gtgcggaaac caacaagatc ctccgccggg aaacggcgcc agtgccgatt gatgggctgt
     3721 gtgtgcgcgt gggcgccatg cgttgccaca gccaggcgct gaccatcaag ctgcgtcacg
     3781 atttgccgct gacggatatt gaagccatgc tcgccgcgca caacccctgg gtagctgtcg
     3841 tgcccaacga gcgcgaagcc agtatgcagc aactcactcc ggccgcggtg accggaaagc
     3901 tgcaggtgcc ggtgggtcgt ctgcgcaaac tctatatggg gggcgagtac ctttcggcat
     3961 tcaccgtcgg tgaccagttg ttgtggggtg cggccgaacc gttgcgccgg atgctgcgga
     4021 ttctgattga aaaccgggcc ggctgacagc gggtggccct tggcttgctg cacaattgag
     4081 cgaaattttc ttacccaaac gcaaaatatc gtatatcctg agcgcatttc ctttaacttt
     4141 ttgccgcaac tttgtgattt gatggataat agcggcaaag ctcttccggg tggcgtggtg
     4201 atggaggagt aagggagagg caaacggccg aaaatttggc ggggatggct gagggggaag
     4261 gcgcgcccat ccgtgtcttg cggacgtttt cggaacaatc agatcgaggg gttgtgacaa
     4321 tgcgtggaaa gaactgggtg tcaggcgttt cgctcgccgt catgttgatg gggcaatccg
     4381 ccgcatgggg agccgggctg ggcaagcttt cgctgaattc tgccctggga cagcccctga
     4441 gcgcagacat caatatcctt ctggccgatc cggccgagtt caataacctc agtgccagac
     4501 tggcttcgtc cgatgcgttc caccgcgcca gcgttgaaat gaacagtacg ctcgaatcca
     4561 ttcatttcgc cctggaaaaa aagcctgacg gtaccgccgt actgcacctg tcatcgagca
     4621 agccggtcga ggacccgttt ctcgacatga tcatcgagct cgactggagc aacggtcaga
     4681 tgctgcgcga atacacggtg ctgctcgatc cgcccggcgc cggcatccct ggtgagcaga
     4741 cgactccgaa actggtggtc aacccggcaa cttccgctgc agtgcccgca agcgcgcacg
     4801 gcgcgcaacc atcgcccgcg gttcagccgc aggcagcgtc tcaacggccg tccgcggcag
     4861 gcgcgccggc agtcaagacc attcgcgtcg aacgcggggc cacactgatt tccattgcca
     4921 gtcaggtcag gccggaaggc gtgcatctgg aacagatgct ggtcggcctg tatcggagca
     4981 atccgcaggc gttcgatggc aacatgaacc ggctcaaggc cggtcaggtg ctgcacgtgc
     5041 cgggcaagga cgtgctgacg tcgatcagcg agacgggagc cttgcgcgag attcacctgc
     5101 aggctgaaga ctggcatgcc taccggcaga agctggctgg cgaggttgag caggcgccgc
     5161 agcggagcgc gagcgccaat gaaagcagcg gcaagattgc gccggccgtg caggaccgat
     5221 ctgcccaggc ggcgcctgcc ggacagggtg tgctcaagct gtcgcgcgga gcagcgccct
     5281 ccggcaaggg gggtgtgtcg ggcgatgaga cggcagccca gcagaaggcg ttgcaggatg
     5341 cggattcacg gctggctgcg ctggaaaaga acatccagga catgcaaaca ctgcttgcca
     5401 tgaaggatca gcagctggca caggcagaga agcagtcacg aaccgcagct ccggccgcgc
     5461 ccgtcccggc tccggcaact gcgcctgcga tgcaggcgcc caagcctgta accgaggtca
     5521 agccaccggt tgcgccggaa atccatccga aaccgaaacc ggtgcatcat gtgcccttca
     5581 agccggtaca ggctccgcct gcaccagcat ggtaccagat gctggatccg cgttatctcg
     5641 gcgcagccgg tggggtgttg ctgctgggcg gcgcactgtg gtggatggca cgcaaccgcc
     5701 gtcgccgcga caacctgtca cgatttgaaa acagcattct gaccagcgtg gattccaaac
     5761 cgaatacggt gtacaacacc cagggtggag aatcggtcaa taccagcagc aatacctcgt
     5821 ttctgacgga tttcagccag tccggactgg ggacgctgga cacccatgat gtcgatccga
     5881 tcgccgaggc cgaggtctac atggcgtacg gtcgtgatgt gcaagcggaa gagatcctgc
     5941 gcgaggcgat gaccaaggaa cccaaccggc tcgagattcg cctcaaactg ctcgaaatct
     6001 acgctggacg cagtaacttg ccggcgttcg agagcggtgc aaccgaactg tactccatgc
     6061 tgggtggaca gccttcaccg ctttgggatc gggcggcgga aatggggcgc aagctggatc
     6121 cacataatcc gctctatggt ggtcaggcat cagaggtggc ggtcgagtcg cctgtggccg
     6181 ctgctgctgt ggctgccgtc gcggccgaag cgttcgatca cgaaaccgct cacgcggccg
     6241 tgaccgaacc ggagagcgag ccggaacaga ccacggctga agcaccgatg gaatttgtgg
     6301 cacaagcccc ggaagctgcc tccgcagcag cggtggagga aacgccggct gaaaccggag
     6361 cggaattccc tggcgccctg gatttcgatc tcgagatcga accggaacac ccacacgaaa
     6421 ccgtggttga agcgagtgac atggagccgg ctgatgccgg tccggcccat gcagatgaag
     6481 atgagcctca gctgacctgg gattttgttg cgccgtcggc tgagaaagct caagcggacg
     6541 aaaccgggct tgccggggaa cacctgtctg cagaatacga accggcggaa gcacacgatg
     6601 cggttgccgt ggaagaggcg ggcgaggaag cgagtccgac atttgcgccg gagttttctg
     6661 gtgtggaagc cgggttcaca tctgcggcac cggtaccgtc agaagcacat gatgcgcacg
     6721 cagtgcctga aattgaagcc gtgtccggga tggaagccgc gtacgagccg gaaagcgcga
     6781 tcgcgacgac agccgaatcg gaaatggaag ctgtgcctga aactggtgcg gctcatgggg
     6841 aggacgctga accggctgca ctggagatgg gtgagcatgc cgaggagccg ctgatgctcg
     6901 atttttccgg aatcaacctc gatttcgggg atgaggcagc cgctccggcc gaggttgcgc
     6961 cacaggccgg agacgagaca gcatctgctg ttgccgagac cggacaaggt gtcgacgtga
     7021 ccggacatcc ggaaggaacc gctctgagtg agtctgagca ggaggtcgga accaagttcg
     7081 agctggcaca ggtctatcag gagatgggcg atatcgagaa cgcgcgcgag atcctgcagg
     7141 aagtgatcgc cgaaggcaac gaacggcagc gcagcgatgc cgaagcgttg ctgcaacaga
     7201 tggataatgc gtcgagctga atccgcgcgt ctgccctgcc atccagccac agccttgctg
     7261 ctgtggctgt tttttattgt ctggatgcag caggtggggg tgcatcaatg gctgaccgcc
     7321 gcggtcgtgc tgacgccggt gttgtggcgc gccggatggc agcaatggct gcgttatctg
     7381 cgtcgcagcc gctggctgct gctggcgatc gtgcttgcaa atgcgtgggc cttgcccgga
     7441 acgcctctgg taaatatcga cctgctttcc gggttgacgc gcgagggaat atctggcgca
     7501 gcgcagacag gcgggcggct tgtgctgctg ctgatgtcgc tggcgatctg catggcacgc
     7561 atgacgcgcg acgattggct ggtggcaatc gataccgtat tgtcaccatt gcggtgtttt
     7621 ggtgtgccag tgcgacgcgt ggcgttgcgt atcggtctga cgttgttcta cgccgagcgg
     7681 ttgctggccg atcgtagcca gttgccgtgg cagcagcgtt ttgcgcggct gttcgatttg
     7741 cccgaagccg atgcagaccc gcagcgatcg atgacctggg tggcgcggcc gttcggctgg
     7801 cacgacgccg tgtgtctggg tggcgtggtg gcggcagggg tggggatcag gtggttatgg
     7861 aattaacgcg tatcgcgctg gggctggaat acaaagggac ggcgttttgc ggctggcagc
     7921 gccaggcgca gggctacagt gtgcaggatg cgctcgaaga cgcattgttg caggtggcgg
     7981 ggcagaccgt cgaggtcgtg ccggcaggac gcaccgatgc cggcgtacac gccagcgccc
     8041 aggttgtgca tttcgagacc agggttgccc ggccgcttac ggcctgggtg cgcggagtca
     8101 acagttttct tccggccagc gtagcagtgc tgtggtcgcg tcgcgtcgac gctgcattcc
     8161 acgcccgctt cagcgcacgt tcgcgcagct accgctacct gttgctgaac cgtccggtgc
     8221 gacctgcgct caacagtgag cgctgcggat ggtatcatgg cgaacttgat atcgagcgca
     8281 tgcgtcaggc agcgtcgctg ctgctgggtg agcacgattt cagtgccttt cgcgcggcag
     8341 aatgccaggc ccgttccccc gtgcgtctca tgcgccacct ggaggtgcga cgacagggcg
     8401 aatacatcgt gttcgacctg actgcgaacg cgtttctgca ccacatggtt cgtaacattg
     8461 ttggaacgtt gctctgggtc ggacgtgaca aacggccggt cgagtggatg gaggagctgt
     8521 tacgcagccg tgatcgcaca caagccggac cgactgcagc agcagcaggg ttgcatttgc
     8581 gtcatgtcga atatgatcca tcctgggggt tgccgctgcc ggttgatccc cgttatgaca
     8641 ttgggcagct tgactgagga gacgcaaggt gggttctgtg cagcgcaccc gtatcaagat
     8701 ctgcggactg accgacccgg acatggcgca gtttgccgca cgcagcggcg cggacgccat
     8761 cggtctggtg cattatgcgc ccagcccacg ccacgtgggg ctggacatgg cggcggtcat
     8821 tgcgcgcgca ttgccgccat tcgtctcgcg cgtggcgctg ttcgtcgatg ccgcaccgga
     8881 ggtggtgcgt gaattcatcc gtgcggcaca acccgatctg ctgcaattcc atggtgacga
     8941 gagtgcagcg tactgccagc agttcgatcg cccttatatc aaggcggttc gcatgcaatc
     9001 cggtgtggat ttgctacaat atgcggctga ccacgcgcag gcgaaaggct tgctgctcga
     9061 tgcctgggtg gacggagtgg caggggggag cggggtggtg ttcgactggg cgcgaattcc
     9121 ttccggcctg ccgctgccgc tgatactgtc gggtggcctg gatgcggtca atgtcggtga
     9181 ggcaatccgc cgggtacggc catgggccgt cgatgtgtca agtggtgtgg aacggcagcg
     9241 cggcgtcaag gatgcggcga aaattgtgca atttataacc ggagtgaacg atgcgcaggg
     9301 gtgaatttcc tgacgaatcc ggccattttg ggccatatgg cggtgttttc gtcgcggaaa
     9361 ccctgatgga ggcgctggaa gaactgcgcc gcgaatatct gtgtgcgcgt gacgacgcgg
     9421 cattcatggc ggaattccgc catg
//
LOCUS       DAIANL010000134        21076 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_169627, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000134 DAIANL010000000
VERSION     DAIANL010000134.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 21076)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21076)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21076
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169627"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4062 a         5817 c         7065 g         4132 t
ORIGIN      
        1 aagacctgct ggctgcaggt gcgcgcgaag accggcttga agccaaagta ttcggcggcg
       61 gcaatgtggt gcggggattc ggtcagatca atgtcggtga gcgcaatgcg catttcgtgc
      121 tcgaatattt gcgccagcgg cgcattccgg tgctggcgga agatctgaac gacgtctatc
      181 cgcgcaaggt gtatttcttt ccgcaaacgg gaaaggtgtt cgtgcgcaaa atcagggagc
      241 tgcgcaatga tacccttgcc atgcgagagc gtgaatatgc tacccggctc aagtcgggag
      301 cagatgggaa acaataggat gatgaacgca tgctgcgctg ggggggcgcg cgtacgggca
      361 gcgcaggatg aaactggacg gatatgatga ttaaggtggc catcgtagat gactcggccg
      421 tgatgcgcgg gctgctcgcg caactgattg atgcccagcc cgacatggaa gtggtgggca
      481 tggcgccgga tgcgtttgct gcacgcgcgc tgatcaaggc gaccaacccc gatgtgctta
      541 cgctggatgt ggtgttgccg gggatgaacg gactggattt tctcgagcgt atcatgcgac
      601 tgcgtccgat gccggtcatt atggtgtccg cgcatgcgct ggcggattcg gatgtgacca
      661 tgaccgcgct cgaactgggt gctgtggatt tcgtcaccaa accgcaggcg cacagcatgg
      721 atgctctgga gcaatatgcc gaagaactgg ctgacaagat tcgtgcggcc gccagggcga
      781 acatcgaccg tcatgaagca caggtcgcgg cgagtgcgcc ggcgcgcgtc aaccgcaggc
      841 tggggtggga aaacgtaatt gcaatcggcg cgtcgaccgg gggaacggag gccatcaggg
      901 aaatcctggt gcgcatgccc accgactgtc cggccatact ggtaacgcag catatgccac
      961 cgggtttcac ccagtctttt gctcaacggc tgaatgactt gtgtaaaata tccgtgagtg
     1021 aggcaaagca tggtcagccc gcgcgtggtg gtcatgccta tatcgcgccc ggacattcac
     1081 atctgctgct cgaacgggaa ggctccggtt acctgctgcg gcttgatgcg ggcgaacagg
     1141 tcaaccggca ccggccatca gtggacgtgc tgttccgctc ggttgcggcg caggcgggca
     1201 aacatgccat tggtattctg ctcaccggta tgggtaagga cggtgcgcag ggattgctcg
     1261 agatgaaaca ggcaggagca agcacatttg cccagacaga agcgtcgtgt gtggtgtttg
     1321 gcatgccgcg cgaggcgatt gcgctgggcg ctgcggatga agtgctggca ccggctgaaa
     1381 tcccggagcg cgtcctggcg ctgctgcgca gtcatggcag catggaaagc ggcactgtcg
     1441 gttatggagt aagaaaatga atgagcgcaa atcaataagc tgggagtttt aaggatggct
     1501 gatccgaaca tgaagtttct ggtggtggac gatttttcca ccatgcggcg cattgtcagg
     1561 aatctgctca aggaactggg ctattccaac gtggaggaag ccgaagatgg cgccattgcc
     1621 ctgtccatgc tcaagagcgg ccatttcgag tttgtcgtga ctgactggaa catgccgaac
     1681 atggatggcc tcacgttgct gcagaacatc cgtgccgatg cgtcactgtc gcatttgccg
     1741 gtgttgatgg tgactgcgga agccaagaag gagaacatca tcgcggcagc gcaggcaggt
     1801 gcgaatggtt atgttgtcaa accgttcacg gcggcgaccc tggacgagaa actgtccaag
     1861 atatttgaga aactcggaaa atagcggagg tcgcatgggt aacgacgata ataatcagca
     1921 gcaggcaccc gccgcatcga tcaacgatga attgctgtcg cgggtgggcc agatcacccg
     1981 gacgctgcat gacagtttgc gtgcactcgg gctggacaag gtggttcagg atgccgcgcg
     2041 ggacattccg gatgtgcgcg accggctgaa ttacgtgatg tcgatgaccg agcaggccgc
     2101 gcaacgcgtg ctcaatgcca ccgatacagc cattcccttg caggaacaga tcgccagagc
     2161 ggccaccgcg ctgtgtgcgg acagtaccaa tgccagcctg aagcaggagc tgacggagat
     2221 tgccggcaag gccgacagga ccaagcaaca gttgatggat atcatgatgg cgcaggattt
     2281 ccaggatctc accggtcagg tcattcgccg catcattgaa ctggcgcacg aactggaaag
     2341 ccagctggtg cagatgctcg tcgattatgc accggccgaa cagcgcaagg aagcgaacgc
     2401 aggcctgctg aacggcccgc agatccacaa ggaccgcgaa gacgtggtgg ccgatcagga
     2461 gcaggtggat gatttgctga gcagcctcgg attctgacct gccagccgct ggggcgcgaa
     2521 ccggtgggca tgaagcggtg cggttcgcag ttcgcaccga ggcagcatgg gcgggttgcg
     2581 tcaattcagg ccagtgtccg gcctgcgtcg attgtaaata atgacagtca ctccgccaga
     2641 cggcggacgg gagacaaacc atgaatcggg aacgaatggt ggtgcgcgat ccattgctgg
     2701 atccgcgcca gcagttgctg ggctaccgct tttactggtc ccgcctgtcg tctggcgagg
     2761 taagtgaagt cgacgaagca cgcagtctgc tggccagatt gcaggaagtg ctggcgagca
     2821 acgaggagag cgagcacccg gtgacgttcc gttaccgttt ctacattccg gcgactgccg
     2881 atttgctgca cgaggaggcg tttcaggcgc tgccagttgc caataccggc ctcattgtca
     2941 gtccggaaac gctggatgac gaagccgcgc gcggggcgct gatgacgttg cgtgcccagg
     3001 gcttcgaaat cctgctgcat ggcgctgatc cgggagggtt gcgtccggaa tggctgcagg
     3061 tatgcagcat ggtcgatgtg catttcgaac cgggcaattt tacgacccag gcacgcatct
     3121 atgcgctgct caagcagtcg tcgatgcgca tggcagctac cgatgtggcc agctggcagg
     3181 aatatgccgt atgcaccaag ctgggactca atgccttcgt tggcaagttc tacatgagcc
     3241 cgccgccatc cgcgggcaac aaagagcgtt cgctcaatcc gtcgcaggcc acactgctgc
     3301 aactgatgga aggcgtgcgc gccaatgcgg acatcgcccg gctcgaagag ctgctcaagc
     3361 atgatgcggc ggtgtcgtac aagctgctgt tctatatcaa ttctgcggcc ttcggcctca
     3421 gcttcgaaat cgaatcattg cggcatgcga tccagatgct cgggtacgag cacctgtacc
     3481 gctggttgtg cgtgctgttc gccacttcgg atgagtcgct cagttcgccg gtactgttgc
     3541 aggcggcgct gatccgtggt cggctcatcg aactgatcgg caagatctgt ctgtccaagg
     3601 cggactcgga aaatctgttc atcaccggca tgttttccat gctggaccgg ttgctcggca
     3661 tcgacatgga ggtggcgctg gaaaatatcc gtctaccgga agccgtcgtg gatgcgctga
     3721 tcggacacac gggcatttat ctgccgtttc ttgaactggc gctggcgtgc gaaaccgaca
     3781 gcagcgatat tggcttgctg gccgacggcc tcgggatcga tcagcggcaa gtcaaccagg
     3841 cgcatttcga agcgattttc tgggcgcaac gtctgctgta acccgcgtat tcccgcctgt
     3901 cgtgtcattt gacgatgcgg cacgcctcgt ggaagtcggc aatgtcgagg tcgaggtcgt
     3961 cagccgtccc gacgttgaac aagtggcgcg cgtcggcgaa ggaaaattcg ccgcgttcca
     4021 gttcctgggc caatccgatc agcatcatga tgtcacggta gactggcagg tcgaagccga
     4081 cacgcttgtg ttcgggattg gccagcaaca ggctcttcag gtagtcgatg gcttcggagg
     4141 gtgtcttcca gcattcggtg atgttgcgga tcaggtgggg atagtcctcg atctgccgca
     4201 ggtcgagaat ggcagggtct tcatcgtagt cgatggtgcg tgcattgaac aggctgctga
     4261 cgcggtcacg aagctgttcg tatttgtcac gggcaccggt cacgcggtac aggtcgagca
     4321 ggtagagcca cggcgcgggt gactcctgca ggggagatgc gctgtcgatg gtttgcgctt
     4381 cgagcacctc gatggccttc tgcggattgt tgaccgacat ccagaactcg gcttcctgac
     4441 tgacatcgga gatttcttcg acttccatgt tggcggaggc gttggcctgc attgccgaat
     4501 gcgcctgcac gggggagcgg ccggaatctt gtgactgtac cgggcgtgat acgggctcgg
     4561 gttcgacagt ctcgaatgcg ggcaaaggat attcgttcgg atgcatgacc ggttccgagg
     4621 caacttctgc cagcggcaag gcattcggac gcacggaggc cggcttcgcc atcggtggca
     4681 cggagggcgt gaccgcagcg ccgtcttcca gcagggcctg atgctgttgc tcgcggatgc
     4741 cgggcattct tcgtgcaagc agcaccagcg ccaggccttc ggcagccacc aggccaatga
     4801 gaatgacgaa ccagttctgg ccggaattca ccggaagggt tgccggcaaa ctggctgcgt
     4861 ggtgctgcaa ctgtgcgtgc aggctgtcga tctgatgctg caagctgatg atatcctgct
     4921 gtttcagccg cagctgcgca tcggctgttg ctgccgggtt gtcctggtgt tgagtcatca
     4981 tctgactcag gtgcgcacgg gcagcgcgca gctcggccag atttgatgca ctctggactt
     5041 cgagctggct gatgagctcg ctgctcatgc gcaggttgat cccttgcggc ggtatggagc
     5101 ttcccgtgtc atcgctgagc gtgagtcggg gttgcgcgct ggcgtggacc gctgccacgt
     5161 gccggttacg ggggtgggcg cgggggtgca gcgttgccag cgacgggctg tgatgcgctg
     5221 cacgtggagc acggggcatg acggaggtcg caggcatgtc gatcgccacg ctggtgccgg
     5281 tcggggttgc ggcctgggcg gggggggatt catccgactg accgggcatg tccagcagca
     5341 cagcatattc gcgctggtag tagttgtcgg agcaactgac gctgaccttg atgtaggctg
     5401 ccggctcgta gagcgattta cgcgtggcaa gctggatggt cgcgttgcca tccttgtcgc
     5461 ggttgacctg tgcctgtgca ttcatcagca actgatgatc ggcagaatcg atgtgtacat
     5521 tgatgcagcg ggaatcgagt gccggatcgg catcgtagac cgcgatgcgg gcgtggacgg
     5581 gctggccgag gccggattcg agctgaatgt ttccgagccc gacagcatgt gccgaaacgc
     5641 tcagcgcgct caccgccagc cagggcaggt atagtgacag tctggcgaaa tggtttaacg
     5701 ctagcggtcg cgacttcatc atcagtatgc caaatgtcac catgcttaaa gttcaatgac
     5761 aaattgtaca taaaattgaa gtgcaagatg aagaaatttg atgtggtgtt caagcaactg
     5821 tggcttgtgc cgtgaaattc aaacacaaaa ccactttacc gttctgtggg ggcagccgcg
     5881 gttgcactgt ttccatacag acgttgccgc agcagatcga gcacgccacg cagtttctgg
     5941 ccggcgcgca cgccatcccc gtgttccagc gcctgcagta cgtcgatgtg cagccgggca
     6001 tactcgggca gacttgcgcc cgcacgcagc gcggcgtaat gaacgcgggc ttcgacgtgc
     6061 atattgttcc agatgcgtag aaactggctg ttgccggatt gcacgacgat acgatgatga
     6121 aagtgatagg cggcgcgcgc gtagccggcc gcgtcgccat tggccgccag ttgctgcatg
     6181 cgttccagag tggtgcgcag gtcgtcgagc atttcgcgcg gacaggggac ggccagttcg
     6241 gcggcacgct gttcaagaat ggcgcgcaat tcgtaaagtt cggccagttc cgtactgctg
     6301 atggcgcgta cgcggctgcc gcagtagcgt tcggactcga tccatcccat ggcttcgagc
     6361 tcgcgcagcg cttcgcgcac aggtgcctgg ctgacctgga actcccgtgc cagcgtcagt
     6421 tcgaccaggc gttcgccggc ggcataatgg ccgtcgagaa tgcgctgcaa cagtctgtcg
     6481 cggatgtggt cgcgcatgca gcgccggtca tggggagtgt cgcaacggtc cggatcggtc
     6541 atggtgtgct gttgccctgg aaggccgcaa gtgtgcggga ttgccagccg ccgcccaggg
     6601 ctttgaccag cacgatatct gctgtgacct gacgcatgtg gatggctgtg gcggtaagtt
     6661 tggcctgggt cgcctgccgg gtggcggtca gtacgtcgag ttcgctgaca acgcccgccc
     6721 ggtagcgata ttgcatctgt tcgagcgcct gctgcgcggc gtgctgcgca cccagggcag
     6781 tctgcgactc gtcgtcgagc tcatgcagcg atgacaggct gtcttcgaca tcgcgccagg
     6841 cggacagtac gctgttgcgg taattggcca ccgattcgcg ccaggccgcc tgggcctggt
     6901 cactcagcgc ggccagttgc cccccgtcga ataacaccat cgccgcgccg ggcccgactg
     6961 accagaaacg gttgggtgcg gtcagcagat tgttgagcgt ggtgctctcg aaacccgctg
     7021 aggcgttgag gctgaacacc ggaaaaaaag cggcacgggc aacgccgatc gtggcattgg
     7081 ctgcggccat gcggcgttct gccgctgcga tgtccgggcg tcgctgcaac agggtcgagg
     7141 gcagtcccgg atcgggtcgt gcgacacgga tgtcgaatgc gggatcgccc ggggcgatgg
     7201 agaaaccgga cggtggctga ccgcacaaca atgcgatcgc gtgttcgagc tgcccgcgct
     7261 gcagtcgcag ctcggcttgc tgggtgatct ggttctgcag cgcgaaggcg gcctgggcca
     7321 ggtcggtctg gttggcgttg ccgcggtcga acatttgctg tgtcaagtca agattgtcgt
     7381 gccagttggc gatcagctgg tcaatcagcg ccagttgggt gtcgacgccg cgcagattga
     7441 aatagtccat ggccagttct gcctggctcg acagcgtggc ggcggcgaga tcgtcttcgc
     7501 tggcaatggc cagcgcgcct gcagcccgcg cagcgttgcg cagccgtccc cagacgtcga
     7561 gttcgtagcc cagatcgacg cccagctggt tatcgcgcca gttgcgcggc acagtggagg
     7621 gaaacagcgg tcggccctgc gacatgcgat tatcggtgtg ctgcgcgtag gcggtgacgt
     7681 caggatagag tgtggcatcg ctggcgcgcg cgatggcgcg cgcctcatcc acacgagccg
     7741 cggcggcagc aatgttctgg ttggcctgtg ctgccagcgt ttcgagtgca tcgagacgcg
     7801 catcgccaaa ggcagtccac cagtcaccgg caggcgtgga tgaccgggcc ggaatccatt
     7861 ggccggcttc cttgtactgc tgcgcagtgt tgattgcggg cggctggtag ggtggcgcca
     7921 gcgagcaacc cgacagtccc agcatcagca gcgtcagcag tgtcagacga cgtttcatga
     7981 ctgttcctgt ttcgggaaag cgaccatgcg cggtacgacg gacatgtcgt cgctgatcga
     8041 atccggcggg ctgacgatga cgcggtcagt cggcatcaac ccggcgcgga tttcgagcgt
     8101 gcggccgaaa tcgcgtgcga ccctcacccg acgcagatgg acacggttgt gtgcatcgac
     8161 gatggcaagc tgggtgccgg aagcattgaa aatcagcgcg ttggaaggca cgagcaatcc
     8221 ggccgaccgg ctgggacgcg gaaagcgcgc ctccacatag ccgcccggca gcaggctggc
     8281 atcggggttg tcgagttgca gttgcacgag cagcgtgtgg ctgaccggat cgatggcgcg
     8341 tgcgctgtcg gtgaggcgcg cttgccacac gcgatcgggc caggcacggg ttgcgaggag
     8401 gatgatgctg ccgacatgca ccagtcgggc ttcggtttcc ggcaccggca catagacgcg
     8461 catgcggtgg atgtcggcca cgtgaaacag ttcgcgggca ttgccgttgg ccgaaccatt
     8521 gtcgatcagt gtgccgatat ccacgttacg ggcagtgatc cagccatcga ccgggctggt
     8581 gatgtgctgg tagacatcca gcgcgcgcag gtgggccagg ttggcttgtg ccgcgtgcag
     8641 gctggccagt ttggcagctg catcgtcgtt cttgctgtcc acatcctgta acgagacggt
     8701 atgcgtcctg agcaactcct gccagcgttg cgcggtgact cgggccaggt catagttggc
     8761 ttcggcggtg cggacatcgg cttcggcttg ccggacctgt tggtcgactt ccggaatggc
     8821 gatgtcggcg atcgcctggc ccaccttgac atggtcgccg atgtcgacca gccagcgttt
     8881 gaggtaaccg ctggtgtgtg cgtagaccgg aatttccgtc caggcttgca ggctgccggg
     8941 cagcaccaga tcgggcaggt tgttttcggc ttgaggcaat acgatgccga cgtgtatggc
     9001 ggcgttgcgt gcagtctcgc gcgccagagc gtcgtgcgcc tcgctgcggc tgtaaatgcc
     9061 ccaggcggca gccagtacag ccagcgcaag cagaagcagg ccgtagcggc gcgccatgcg
     9121 aatcgtcgag tggtgcgggg aaatggggga tgtcatgcgc tgatccttat ggagcgacgg
     9181 gtttcccggt gcggcggcca tgcagcacgc tgaacaccac gggaacgaag aacagggtgg
     9241 caagggtggc aaacagcaat ccgccgatga cggcacgacc gagcggggcg ttctgttcgc
     9301 caccttcgcc gaggccgaac gccatgggcg ccatgccgat catcatggcc agtgcggtca
     9361 tcaataccgg gcggaagcgc gagtaaccgg cttcgagcgc ggcctgcagc ggtggtacgc
     9421 cgtcatgcag gcgttcacgg gcgaaactga cgaccaggat gctgttggcc gcgcccacgc
     9481 ccatgcacat taccgctccg gtcagtgccg gcacgctaag tgtggtatgc gtgacgaaca
     9541 gcatccatac gatgccggcc agcgctgcgg gcaaggccgt gatgatgatg aacgggtcga
     9601 gccaggactg aaagttgacc acgatcagca gatagacgag cactatggcg ccgagcaggc
     9661 cccactccag gctggtgaac gaggattgca tggtctcgac ctggccgcgc agcacgatat
     9721 gggtgccttt gggcaattcg tgctgcatcg attgcactgc ggcgcggatc tgctgcgcca
     9781 catcacccag atcagtgccc tggacgctgc cgaaaatgtc gattaccggc ttgacgttgt
     9841 agtggctgac cacagccgga ccctcgctgc ggctgaatcg cgccaggttc gccaacagtt
     9901 gcggctgggt tgcgcctgcg ccggtgactg gcacctggcc cagatcttcc agcgtttgca
     9961 tgcggtattg cggcacctgt gccgcgaccg agtactgtac cccggtgtgt ggattggccc
    10021 agaagctcgg gttggtctgg aaactgcccg atagcgaagt cagcaggttg gtggcgatgt
    10081 cctgttccgt gtagccaagc agttgggcgg tgtcacggtt gacggtcacg ttgatttgcg
    10141 gatagttgaa tgcctgctgg atgcgcaggt cgacagtgcc cggaatggtt ttcagtcggg
    10201 cgagcaaccg gttggcgaat gcgcgatttg cggtcaggtt gttgccgatg atctgcacat
    10261 cgagcggcgc cggcaggccg aaattgagaa tctggctgac gatgtctgcc ggcaggaagg
    10321 caaacgtggt ggtgggaaaa tgcgccgtca gccgctggcg aaggtcgtgc acgtattggg
    10381 ccgatggtgc gtggttcgca ttcagcgaga tatagatgtc ggcatctccc ggaccgatgg
    10441 gcgcagaggt gctgtaggac agattgatgc cgctgtaagg caggccgatc acatcgacga
    10501 tgctggataa ctggtcggcg gggatgttct ggcggatctc gcgttccact gcatcgcaca
    10561 gggcggctgt gtcttcgatg cgggtaccgg tggcggcacg caggtgcagc ttgatctgcc
    10621 cggcatcgac ttcggggaag aagttctcgc ccaaccaggg tgacaacgcc agcgataacg
    10681 caacagcaaa cagaaatgcg cccatgaagc cacgcgggtg ggccagcgct gcggcgagca
    10741 attggcggta atcctcgcgc agggtcatga atcccgcttc aaagcgtgcc tgcaggcggg
    10801 ccgccatgcc acgacgggcg gcaatctgtt ccgggctgtg catgtgcagc cagtatttgg
    10861 ccagggtggg caccagtgtg cgcgacagga tccacgaagc cagcatggcg aataccaccg
    10921 attcggccag cggcacgaac aggtagtggg ccacaccggt gagcaggaac atcggaatga
    10981 acacgatgca gattgccagt gtcgacacca gagacggaat ggcgatctgg cgcgcgccgt
    11041 cgagaatggc gggttcgacc tctttgccct gttcgagatg ccagttgatg ttttcgatgg
    11101 ccacggtcgc atcatcgacc agaatgccta cggcaagcgc gagaccgccc agcgtcatga
    11161 tgttgatggt ttcacccagc cacgacaggg tgatgatgga ggccagcacc gacagcggga
    11221 tggaaatggt gatgatgagc gtgctgcgcc acgatccgag gaacagcagg atcatcagac
    11281 cggtcagcgc agcggcgatc acaccttcac ggatcacgcc gttgatggct gcacgcacga
    11341 agatcgactg gtcgccgagc gcatggatcg tcatgccggc aggcatgtcg tcacggatgg
    11401 acggcagcat gcgtttgatg tggtcgatga tgctcagcgt ggaggccgat ccggtcttga
    11461 gcacgcgcat cagcacggcg cggtgtccgt ccacacgcac gatgttggtt tgcgggggtg
    11521 ccccgtcatg cacatgcgcc acgtcgcgga tgtggatcag cacaccgtgc gctgcccgca
    11581 acggaatgtc gttcagtgac tcgatgcgca gaaaactggc attgagcttg atgtagtact
    11641 cgaaatcgcc gattttttcc gttccggccg gcaggatcag gttctggctg gcgatggcgc
    11701 gggtgacgtc atcggccgac acgccgtaat tgcgcatggc gatcgggtcg agatcgacct
    11761 gtacctgacg catctgcccg ccatacggaa agggcagcga tgcaccagcc acggtagcga
    11821 gctgggtgcg cagaaaactg ttgccgaagt caaacagctc ggcttcggtc atggccgggc
    11881 tggacagcgc cagctggatt accggcacgc tggatgcgtt gtaggcaaga atgaacggtg
    11941 gcgtcattcc cggtggcatg acgcgtaaca gggtttgcga gatcgaggtg atctgtgcca
    12001 ccgccaggtc ttcgttgacc cgtggctgga aaaaatactt gaccacgccg atgccgttca
    12061 gcgactgcga ttcgatatgc tggatgtcat tgacggtggt ggtcgcgacc cgttcgctga
    12121 aggccacgat gcggttggac atgtcttccg gcggcatgcc ggagtagttc cagatgaccg
    12181 acaccaccgg aatgttgatg ttgggcagaa tgtcgactgg tgtgcgcagg atgctgaaca
    12241 caccgagcag cacgatgagc agcgccatca cgatgaaggt gtaggggcgt tgcagggcga
    12301 ttctgacaat ccacatcgtg cgctcgcgca aggaaatttt gtgcagtata gattatcgat
    12361 aatttaacgg caagcccgtt gccagagcgg ggaagtccgc gccgggctgg acgaaaacgg
    12421 cacgtggctt ccatggccga agaaatgtgt actatgatgg aagtgcatgt gctgcgctgc
    12481 ggtggacgcc ggcctgatcg acgtagcgtg gctgcgttcc gggacggaat cgtgacgggg
    12541 gaggtcatga cggatgatat gggcaatatt gttcggtcgc gccagactcc tggcggcgag
    12601 tggccagacg ccggttactg gttccgttcg ctggtggaga gcatcaatga agtgttctgg
    12661 ctggcatcgg cggaccggcg gcggtttcag tatgtgagtc cggcgttcag gcagatctgg
    12721 ggtaaacaca gttccgatgc cgaggtggtg gcgaaattgt ggcgccaggc ggtgctgagc
    12781 gaagaccggc cagtgctgtc gcgggcgttg cgtagcgtgg cccggcgtgg ccattacgac
    12841 atcgagtatc gcatccgccg ccccgacggc gatgtgcgct ggctgcgcga acgtgccatc
    12901 ctggtgcgca acggtaccgg cgtggccctg catctggccg gggtgaccga tgacatcagc
    12961 gccacacgcc aaagtgcgga aaatctgctg cgtctcaacc gtgcgctgcg tttgctcggt
    13021 gcgtgcaacc gggtggtgat gcatgccgcc tccgaacagg agatgttcga gtcgatctgc
    13081 cgcgagatcg tcgataccgg tggctatcgg ctggcctgga tcggtcgggc gggcgaggcg
    13141 ccggagcggc gggtgtggcc ggtggcgcgc tatggtacgg gggatgatta cgtcgaccgc
    13201 attcacgtga gctatggcga cgaagagatc ggtcgcgggc cgaccgggtc gtcgattcgc
    13261 accgggcaga cccacatcaa ccagaattgc ctggccaatc cttccatgtc gccgtggcgt
    13321 accgaagcga ttcgccaggg gtatctggcg agtatcgcgc tgccggtgcg tgacgagatc
    13381 ggtgttttcg gggcgctgac gatctatgcc tcgcagcccg atgccttcaa cgaggaggaa
    13441 gtgcgtctgc tcgaagaact ggtggaaaac ctcgattttg ccctggccgc actgcatgcc
    13501 cgccggcagc ggcaggaggc ggagcaccgc ctgcgcctgg cggcacaggt gtatcagtcg
    13561 agcagcgagt cgatgctggt gatgaacggt cgctggcagg ttctggatgt caacccggcc
    13621 ttcgttgaac tgaccggcta tgcgcttgtc gaggtgatcg gccgcgatcc ggacttcctg
    13681 ctggccgaac cgcaggagca gacacaggat gacgtgttct ggcgtaccgt gcgccggcag
    13741 gtgcaacagt cgggcgcgtg ggagggcgaa gccctggcgc gcagtcggag tggcaagctt
    13801 ctgccgttgt ggctcaatat cagccgtatt cctggcgagg acggagacga tgctgccgag
    13861 cctcgcttcg tggtgttcca tcatgacctg acggaaaaga aacagttcga ccagcagttg
    13921 tggcgccagg ccaattttga cctgctgacc gggctgccca accgcaacct gctccaggac
    13981 cgcacggcgc aggcgatcag ccaggtcgcg cggcacgatg gcatgatcgc agtgatggta
    14041 ctcaacctgg atgggttccg catgatcaac gacagtttcg gttatgcggc cggtgacctg
    14101 atgctcaggg agattgcgca acgcctgact gccggtgtgc gccgcgagga cacggtggcc
    14161 cgactcggct cggacgaatt cgtggtgctg atgaccgagg tcagcgatga cggcaatgtg
    14221 tcgttcatca ccgaacagtt gcgcagtgcg ctggcgcagc cgttcatgat cgatgaacgc
    14281 gaactgttca tcaccactgg cattggcatc gccttgtatc cgcgcgatgg cgacgatgtg
    14341 tcgacgctgc tgcgcaatgc cgatgcggcg ctgcgccagg cgcgcgagtc cgggtgcaac
    14401 agtttccagt tctacgatcc ggaactgaac gaagcggcgc tcggcaccct gatgctggag
    14461 aataacctgc gctacgcgct ggagcgcaac gaactgctgt tgcattacca gccactggtc
    14521 aacctgcgca gcggacgcat gacgggtgtg gaagccctga tgcgctggaa tcatccgaag
    14581 ctgggcatga ttccgccgac gcgtttcatt ccggtggcag aaaaatccgg ccagatcgtc
    14641 gagatcggtc gctgggtgct gcgacaggcg tgtgcgcagg cagcggcgtg gcgggcgcag
    14701 ggacacgagg tggtggtggc ggtcaacctg tcggcgctgc agttccgcaa cgcggcgctg
    14761 gtggacgaag tggcggggat tctggccgag accgggctgg cagccgaagg gcttgaactc
    14821 gaactgaccg aaggcgtgtt gatccgccag ccggaactgg tgctggacgt gctgaccgca
    14881 ctgcgcaaga ttggcgtacg cctgtcgatt gacgacttcg gcaccggtta ttcatcgctg
    14941 gcatacctca agcgcttccc ggtcaacaag gtgaagatcg accggacatt cgtggccgac
    15001 atcgtcaccg atccgaacga taacgccatc gtcagtgcga tcattgccat ggcgcacagc
    15061 ctgaagctga gcgtgatcgc cgagggcgtg gaaaccatcg gtcagctggc gtttcttgtg
    15121 atgcatgagt gcgacgaaat acaggggtac tattacgccc gtccgctgcc ggccgacgag
    15181 attgacgtgc tactggcgca gccgcgcctg ttgcagttgc ctgaagtggc gcccgggaac
    15241 gtgccgaccc tgctgctgct cgatgacgag gcaaacatcc tccgtgcact ggagcgggtg
    15301 ttccgtcgtg agggttaccg ggtactgagc acgaccagcg ccatcgaagc gttgcacctg
    15361 ctggccggca atgaaatcgg cgtggtggtg gctgaccagc gcatgccgga aatgaatggc
    15421 gtcgaattcc tgcgccgggt gaagcaactg catcctgatt cgatccgcat cgtcctgtcg
    15481 ggctatactg atctgaaatc ggtgacagca gccatcaatg agggtgcagt gtacaagttt
    15541 ctgaccaagc cgtgggacga tacgcagtta caggataaca tccgcgaggc atttgcgctg
    15601 tatgccatga agcgggatca tgcccgtctg gtgcgcgaac tgacgctggc gaacgagcgc
    15661 ctgcagtccg ggcagggagc gatgtaaatg aatgccccca cgctgctgct ggttgacgac
    15721 gaggcgaaca tactcaatgc actcaggcga ctgttccgtc cgcatggtta tcgcgtgctt
    15781 accgcggaaa gcggcgcggc agggctggaa ctgatgagca gcgagtccgt cgacctggtg
    15841 atctccgaca tgcgcatgcc ccagatgaac ggcgccgagt ttctcgaacg ggtacagacg
    15901 gaacatcctg atgtggtgcg tatcctgctg accggttatg ccgatctcga ctccaccatc
    15961 gctgcgatca atcatggcgg catctaccgc tatctcgcca agccgtggga agaaaacgac
    16021 gtgctgctga ctgtgcgcca cgcactcgaa cgcaaggcgc tctaacagga aaaggtgcgg
    16081 ctggagcagt tgacgcagca gcagaacgag gctttgcgac agctcaatgc cgggctggaa
    16141 gaaaaagtgc gtgctcgtac ggaagaaatc cgtcagatca atacgtttct cgaagccgcc
    16201 aacaagcagc tcagggaaaa tttcctgttg tccatgcgcg tgttctccaa cctgatcgaa
    16261 ttgcgccatg gcagcattgc cggccattcg cgccgtgtgg ccgaacatgc gcgagcgttg
    16321 gcgcaggcga tgggcatgac gcagagtgag gtgcagaatg tactgtttgc cgcactgttg
    16381 catgatatcg ggaagatcgg tctgccagac cgggtcatcg tcaaggcata cagccagctc
    16441 gatgacgagg agcgcaaggc gttctggctg catccggcga ccggccaggc cgcgctgatg
    16501 ggcatcgaga cactgcgtga ggccggcagg ctgttgcgta gccaccatga gcgtttcgat
    16561 ggtctcggat ttcccgatgg gctgcacggc agcgaaattc cgcaaggagc gcgcattctc
    16621 gccgtagtca atgattatga tgcgctgatg catggcggga tccagccacg gcagtattcc
    16681 gcacaggaag ccagagaatt cctgctcgcc ggcaagggaa cgcgctatga tccaaaagtg
    16741 gtggatggat tcatccggtt gatgggcatg cacgcggagc cagccgctac cgcagccgtg
    16801 tgtattactt ccgaccgcct caaaccggga atggtgctgg cgaaagatct ggtgagcaag
    16861 gacggcctgt tgttactggc cagggactat atgctggatg aaacgttgat cgggcagata
    16921 cgcggttatg aagaaacgat cgaccagcag atcggcatct acgtcagggc gtagccagtc
    16981 gttgaaaaat gtcgtgagtc tgggcaagat gagacgtaag gcgaaatcca tgtggtctgg
    17041 atgagggttc ccggccggtt tcaacgaaat accggcaaac gcaacagtga ttcaacagcc
    17101 agtcaggagg atggacatgt ggcgcatact ggtcgtggac gatgaagaaa acattctcag
    17161 cgccatccgt cgtggactga agcagtccgg tcgcgacgaa tcgctgccgc cgctgcaggt
    17221 cgaggcgttc acctcgccgc aggcggcgct tgcccgttcg cgcgaaggtg tcgcttatga
    17281 tctggtcctg tcggattacc gcatgccgga gatgagtggc atcgaattcc tgcgttcgtt
    17341 ccgcgagctg cagcccgacg ccgtccgcat cattctcagc ggttatgcgg acctggatgc
    17401 ggtgatccat gccatcaacg aagtcgaaat ctatcgcttc ctctccaagc catggaacga
    17461 tcatgaattg cagcagacga ttcgccaggc gattgaacac cgcaacctgt tgttggaaaa
    17521 ccgccgtctc gccgatctgg tgcgcgtgca gcaaggcaag ctgacgcgac agcagctcga
    17581 actgcgtcgg ctggaggagg aaactccggg catcacccgt gtcaggtggg ggccggatgg
    17641 cgaggtgttg ctggaagagt tctagcatgg cagaactgga cgaggatcag cgttgcctgt
    17701 gggtcaagct ggtgtattac ggcccggcga tgtctggcaa gaccaccaac ctgatgcaat
    17761 tgcacgaaca actgtctccg cagcgcaagg gcgaattgat gacgctcgaa acccgcaatg
    17821 atcgcacact gttcttcgac ctgctgccgc tgggtttcag cgcgccatcg gggctgcagt
    17881 tacgcttcaa gctgtttacc gttccggggc aggtggcgca cgatggtaca cgcaaggcgg
    17941 tactgtcgcg tgccgatggc gtggtgttcg ttgccgattc tcagcgcgcg cagtcggtca
    18001 acaatgggga atcgtttcgt aacctcgccg agaacgcagc acaggtcggc atcgatttcg
    18061 cggtactgcc gctggtggtg cagttcaaca aacgcgatct gccggacatc gtttcggaac
    18121 aggaggtgct cgaacgctgg aagcacgccg gctggccggt gcattttgcc agtgcgaccc
    18181 atggcgacgg ggtgggcgag acgttcaccg cgttgctgca cgcggtgtac cgaccgcttg
    18241 atgcgcgttt ttcgctgcac gatagccatg gattgtctga agatgccttc gtttccggag
    18301 cgcaggtggg tggggtggca tgatggctgc ggcgggccgt ttcgatcgcg atgcggcgct
    18361 ggccgacctg ctggctggcg atgcgctgga gcgggtgcgc gcactgttgc ggcgctcgct
    18421 gggagaggat catgcgctgg tcgatgcccg cggccggctg atgtcagggc gggagcagca
    18481 tgatggtcgt caggtggctc tgcagaccgg aatcgacccg atcgggtatc tgtctgcagc
    18541 aggtgccgac gatgccatgc tgagttcggt agccggctgg cttacggcct ggctggcaag
    18601 cgccgacaag taccgcatgg ccagtgaact gcatctggaa gccgtggcag cggattttgc
    18661 cgcattgcag gcgcgtaacg aggcattgca gcagtcggag gagcgttacc gcatcctggc
    18721 gcaagatctg gaacgacgtg tgcgcgaaca ggtagccgtg atcgaagtcg ctcagcgcca
    18781 gttgtaccag gccgacaaac tggcttcggt ggggcagctt gcggccggaa tggcgcatga
    18841 gatcaacaat ccggtcgggt tcgcccgcag caacctggcg acggccaggc gttatgtcgc
    18901 gctgctgcag caactggagg tacgggtgcg cgatggcgat tctgcggcgc tggcggcgtg
    18961 gtgggcacag gaagacctgt cgttcgtact cgaagacttt tccagtctgg tggcggaaag
    19021 catggacgga ctggacaggg tggcgcgtat cgtagccgat ctcaaggcat tttcgcgcat
    19081 tgatgacggg cagcgccagc gggtggacct caatgacaac ctgcgtgccg cctgccatgt
    19141 ggtggaaccc tggctggccg ggcgcatcgg gctgacgttc gaacccggag cgttgccggg
    19201 cgtcgagtgt gatcccggtc atatcaacca gatgctgctc aacctgttgc gcaatgccat
    19261 ccagtcgatc gaaggcgacg gcctgatccg catcgacagc caggtgtgcg acacgggcgt
    19321 gcagatccat gttgccgaca gcggttgcgg catgtcgccg gaggtgctgg cgcgcgcgtt
    19381 cgatccgttt ttcaccacgc gcccggtcgg tgccggcgtc ggcctcgggc tgacggtgag
    19441 tcgggacatc gtgctcagtc acggcggcac cattgttctg gatagcgtgc ccggtcaggg
    19501 aacccgcgtg accatcaacc tgccagtcag tcgtggagca tgaagtgacg gattattctt
    19561 cgttgtttac cggcgcagca gacaccgcgt ccaccacccc ggtgccggag tccaggccgg
    19621 cgtaccgtgt cctgctggtg gatgacgaaa tcaacgtgct caatgcgctg cggcgggtgt
    19681 tccgtcagga gaactatcag atcgtcacgg cggtcagtgc cgtggaggcg ctggagctgc
    19741 tgcgcacggg cagctttcat ctggtgatct ccgatttcat gatgccgggc atgacgggtg
    19801 ccgaattgct caagcaggcg cgcgccatgc atccggacat gattcgcatc atgctgaccg
    19861 gacatgccga taccggcgcc gtgatgggcg ccatcaacga aggggcggtg tacaaattta
    19921 tcctcaagcc gtggaatgat gacgatctgc gcatcacagt cgcgctggcg ctggaacagt
    19981 atgacctgat tcgtaacaac cgcgccctgc agcaggagaa ccgtgacaaa agcaaggaaa
    20041 ttgccgcgct gacccgtctg gcgatttcca accgcagtca gcttgccatc atgctttcga
    20101 aacgcaatct gctgacctcg caacaggtcc aggaactgca ccggctacag cagacgcaaa
    20161 aggagccgat catccagttg ctgttgcaga aaggctgggt cgaggaagat gccatcattg
    20221 acatcctgcg caaggatttc ctgttcgagg aagtgtcgct ggccgaattt cgcgtcgatt
    20281 cggcactggc ggggatgatt ccgaaaagct tctgcgaacg ccagctggtg gtaccgctca
    20341 agctcgatgg ccgtcgcgtg atgctggccg tggctgatcc gatggatgcc gggctggtgg
    20401 atgatttccg cttcgtgacc gggttcgagg tgcaggtggt gatggcgcgc gtgtccgata
    20461 tccgtgccaa gctggcggaa ctctatggca cgccggacga gattgccttc cgcgatctgg
    20521 aaacgctggt gtccggggtt gacccgtatg aaggcatcga aatcgtcatc gaggaagacg
    20581 acgacaccag cctggacgag ctgctgcgcg gcaccgatgc gccacccgcg atccgactgg
    20641 ccaacgcaat cattctggaa gccatccggc ttggcgccag tgatatccac atccagccgc
    20701 gcaccaagag cgtcgcggtg cgctaccgta tcgacggcgt actgtcggac aagatacaga
    20761 ttcccaatgc gctgtatccg ccgttggtgt cgcggctcaa gatcatgtcg gaactggaca
    20821 tcagcgagcg gcgccggccg caggatgggc gcatttcggt gaaaacaccg ctgcgcgtag
    20881 tcgatctgcg catttccact ttgccgacca tcaacggcga gaaagtggtg atgcgcattc
    20941 tcgatcgcaa ttcggtgatc aagaatatcg gcgaattcgg gttttccgtg cgcgatttcg
    21001 caatggtgcg gcgcatggtg agcatgccgc agggcatcgt gctggctact ggcccgactg
    21061 gctcgggcaa gaccac
//
LOCUS       DAIANL010000135         4851 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1403797, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000135 DAIANL010000000
VERSION     DAIANL010000135.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403797"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          995 a         1578 c         1421 g          857 t
ORIGIN      
        1 acgtcggtct ggcgggatgg attgcagggc gcaattcgca gtcaatggct gccctcctga
       61 caggaaatgc aacgcagcac gccgcacgcc agaccacgtc ccatgcgggt tgcgtaaaca
      121 tcgtgcgccc gttttaccgg atgccctggc gtcgtggtca cggctacgtc ctgcgccatc
      181 cggctcacga tcatcacgat gtgttcagca acgcgattcg cgtggattta atcatcgcgt
      241 ctttgctgct gtttttcgac agcctgttag tgacagacgg attttattgc tataatcgac
      301 cgtctcttca acgaatagac gcaaagcaag aggcctcgcc ggccgttctg gctggcggaa
      361 cggggcactg ccgccgcagt ccgtccagcc ctccggcgtt gccaaaacac caatccggtt
      421 caaacccaaa tcaggagata ttgaagtggc agacaaactg ttaatcatga tggttaatac
      481 ggatccgagc aacggttccg aactcggtgc gccgtttttc caggccacgg tcgctgccgc
      541 catggaatat gaagtcgaag tgatcctgac cgcacgttcc ggcgaactgg ccatcaaggg
      601 cgttgcagaa aagctgttcg ttcaggccgg ctcgcccaaa agcgtgtacg atttcatcaa
      661 ggacgcgcat gaagccggcg tcaagttcaa ggtctgcacc ccgacgcttg aactgtgggg
      721 ccacgacctg atcccggaaa tcgaagaaac cgtcggcggc gcctacgtca tccagcaagc
      781 catggatgaa gacgtggtaa cgctcaccta ctgattcagg ggccgtcgga cgaaccgtcc
      841 ggccgtcgcg cgcattagcc agaaagcgga gcgatcctcc gcttttttcg tgtccgcctt
      901 ctgcggtgcg gctgttgcgg catgaattga acttatttgt cggttacacg gtcagtagtc
      961 acaacaacaa cagggttgca cacacggcgt gtgcggcaac cggccaggga ctgaagcaca
     1021 cgatgaatga tcgggatatc gatcaacagc tggtggaacg tgtacagcgc ggtgacaaac
     1081 gggctttcaa tctgctggtg gccaagtacg aacgacgtct ggtgcgcctg ctgtcccgct
     1141 tgatccgcga tccgcacgag gtcgaggacg tggcgcagga aaccttcatc aaggcctatc
     1201 gggccttgcc gaatttccgc ggcgatgccg ccttctacac ctggctctac cgcatcggca
     1261 tcaacacggc caaaaactgg ctgatggcgc gcggccggcg cgcgccaacc acgaccgcgc
     1321 tggacagcga ggatgcggaa ggcttcgaag acgccggcgg gttgcaggag ctcaatacgc
     1381 cggagaacga gctgctgtcg agggaagtgg cccaggtggt caacagcgcc atggcagaac
     1441 tgccggaaga cctgcgcaca gccatctcgc tgcgcgaaat tgaaggactg agttacgagg
     1501 aaatcgctga aaccatggga tgcccgatcg ggacggtacg ctcacggatc tttcgtgcgc
     1561 gcgaagccat cgccgcccgg ctacgcccca ttctcggcac caaccaggac acgcgatggt
     1621 aaggagtacg atcatgcaag cacgcacacg acacgccatt cccgcaggcg agtggcccgg
     1681 aaccggaagt tccgccaaag aaatcctctc cgcagccatg gattgcgaac tggatgcttc
     1741 ctcccgcgac cagctgttcg ccttgtgcga ggatgacggc aaccggcagg actggctcgc
     1801 ctggcacgtc atcggcgaca ccttgcgcca gacaccggcc tgcggcgacc tgaccgcacg
     1861 ggtggcccgg cagctcgccg gcgaaccgac cgtgctggcc ccgcgacgca aacgcccgac
     1921 cacccgctac ctgatgccgc tggcagcttc ggtcgccgcg gtggcgctgg tcagctggag
     1981 cgcgctgcat gtcagcggag gctccacccc gaccccggcc acacttgcgg ccaacagcgc
     2041 cccggcggca cgccaggtcg ccatgagcag cccacccgtg cagtcctcgg gcatagacaa
     2101 ggcacgcctc gccggctacc ttgcctcaca ccgcgatttt gctgcgggat cgaacgcatc
     2161 cgcgttcatg gatgccacct ggcaggtacc cacggggtct ggccgatgag attctggttt
     2221 gcctgcctgc tgctgttttc cggactgcct gccctggccg atacggttcc cattccgacc
     2281 gaaggctcgg cctggatcga acaggtggct cgcgccacac aacagaccga ctatcagggc
     2341 gtattcgtct accagcacca ggagcacatg gagatttccc acattgcgca ccgtatggac
     2401 cgcaatggcg agatggcccg cctcgacacc ctgtcggggc cgccaaaatc cttcctgcgc
     2461 gaaaacaacg ccgtctactg ctatattcct gacggccgca aggtcaacat cgagcgccac
     2521 cgccaccacc cgttctttcc ggaaatcctg ccgcagtcga ccggcgcgct gctgcagtgg
     2581 taccagatcc ggccgctcgg ccacgccacc atcgccggcc gcgacgccac cggcatggcg
     2641 ctgatgccac gcgatgccta tcgctatggc tacctgctct gggccgacag cagcagcaat
     2701 gtgctgctgc gcatggtcaa actcgatgcc cagggccagc ccatcgcgca attcaccttc
     2761 acccaggtgg aaatcggcac cgcacccgac cgcaggcagt tccaggccaa cttcatcgac
     2821 cgcaagacaa tcagcctgcc cgaccgtgaa acaccgatcc acaccggctg gaaagtggcc
     2881 agcctgcccc ctggtttccg cctgatcacc gagaaccagc tcacgctccc caacaagagc
     2941 cagccggcag tgcacatggt ctacagcgat ggactggcca cagtctcgct gttcgtcgaa
     3001 accgacccac atccgggcgc acaaccgaaa cgcgggttgt tcagcgagaa cgccctgacc
     3061 ttgtacatcc gcacaaccgg cgacaaccag atcacggcac tgggtgaagt accacccgcc
     3121 acgctgatga tgatggctga cagcctggaa aacacaggga aaaaataatg gaaatcgagg
     3181 cccgggtagt acaggtggac acagacggca cggcatgggt caaggcgatc gctggtgccg
     3241 aatcctgcca ctcctgcagt ggcagcagca acggcgagga aggcggcagt tgcgccaccg
     3301 gcaacatcgg ccgcatgttc gccagccgcg accagcgtta tcgggtcatc aacaccattg
     3361 gatgccgcac gggtgaccac gtcattctcg gcatcaccga cggttcggta ctgcgcggct
     3421 cggctgccgt ctacctgctg ccgctgctgc tcatgtttgc cggggccatt gccgccagcc
     3481 atctgctggc cggcgtggcc accgacatgg ccgaagtcgt cggtgcggcg gcaggatttt
     3541 ccgccgcatc ggcatggctg tggtacttca accgcaagat ccggctggat acccgtttcc
     3601 agcccgtcat attgcgtcag gcaagacaag gcattgtaat gatgaaggaa atcagatcat
     3661 gaaaaaatgg ctgcactggt ggttgctttt tctggtactg ggcttcagct ccgcccaggc
     3721 aaaagattat cccgatttca cccagatcgt cagggatcac ggcgctgcgg tggtcaacat
     3781 caccaccaca cagatgacgc gggtgcgcaa catgggcatg cccggcctgc ccgacgatga
     3841 catgttcgac tttttccgtc gcttcatgcc gccgccaggc gcacaggata acggtgggga
     3901 agaagtgcat agcggcggct ccggcttcat catcagcccg gacggctata ttcttaccaa
     3961 tgctcacgtg gtggacaacg cagatgaagt aacggtgaag ctgaccgacc gccgcgaatt
     4021 caaggccaag gtcatcggta ccgacccgcg gtccgacatt gccctgatca agatccaggc
     4081 cactgggctg cctgtggctc ccatcggcga tccacgccag ttgcaggttg gcgaatgggt
     4141 aattgccatc ggttcgccct ttggcttcga gaattccgtc acggccggta tcgtcagtgc
     4201 caagaaccgt tcgctgcccg acaatgacta cgtccccttc atccagaccg acgtggccgt
     4261 gaatcccggc aattcgggcg gaccgctgtt cgacatgcac ggccaggtgg tcggcatcaa
     4321 ctcgcagatc tattcgcgct ctggcggtta catgggcgtg tccttcgcca ttccgatcga
     4381 cgtcgccatg caggtcgccg accagttgcg ccagtacggc aaagtgcgtc gcggccggct
     4441 cggcgtggtc attcaggaag tcagcgccgg ttctgccagt gcattcgggc tggacaagcc
     4501 tgccggggcg ctggtgtcgc aagtcgacgc cagcggcccg ggagcccgtg ccggcctgaa
     4561 gcctggcgac atcatcctgc gtttcaacgg acagcccatc gtgcgctcga tcgacctgcc
     4621 acgcatggtg gcaaacgcca aaccgggcag catcgcacgg cttgacatct ggcgcaaccg
     4681 tgcaccgctt gcactcaacg ccacgctggg cgagatgccc gacaagaccg gcaaccgcca
     4741 tacccgcacc tttccggacg gcaagacccc ggacaaggtc ggactggtgg tcagcaacct
     4801 gccacctgac gcacctgccg acagcagcgg cgtggtggta cagcacagtg t
//
LOCUS       DAIANL010000136         6454 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_785027, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000136 DAIANL010000000
VERSION     DAIANL010000136.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785027"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1193 a         1803 c         2134 g         1324 t
ORIGIN      
        1 atctgttcta ttttaagttt ttcgccggcg ctgatgcgat attgcttgcc gccggttttt
       61 atgaccgcat acatgggatt ctccaagagt acctggggga aacggcggat tataagatat
      121 cctggaaaaa atatcaaaca ccggttgtta ggagttttcc ttgacacaat gcaggctggc
      181 tccctaccat tcacaggttg ggttacaggc gaagcgaagc ggaatcatgc aggcgttaca
      241 ggcgtttctg gctgcggaca tgcagcgtgt cgatgaggtg atccgggcgc gtctgcattc
      301 cgatgtggcg ctggtgggtc aggtggccga atacatcatc ggcagtggcg gcaagcgcct
      361 gcgtccggcg ctcgtgctgc tgacggcggg cgcggttggc tatcgcggca gccatcagtt
      421 cgaaatggct gccgtggtgg aattcattca tactgcgacg ctgctgcatg acgatgtggt
      481 ggatgaatcc ggactgcggc gcggtcaggc cacggccaat gcgctgtttg gcaatgcggc
      541 gagcgtgctg gtcggcgatt tcctgtattc gcgtgcgttc cagatgatgg tgagtgtcgg
      601 tgacatgcga atcatgcagg tgctgtcgga tgcaaccaat gtcattgccg agggcgaggt
      661 gctgcagctg atgaactgca ataacgccga tatcaccgag caggactacc tgcacgtgat
      721 ccgctacaag acggccaagc tgttcgaggc tgcagcacgc atcggtgcgc tgctcggcgg
      781 agcggacgaa acgctcggag cggcgatggc cgactacggc atgcatctgg ggacggcctt
      841 tcagctgatc gatgatgtgc tggattattc aggcgatcat accgaaatcg gcaagaacat
      901 cggcgatgat ctggcggaag gcaagccgac cctgccgctg ctgtacctga tggaacatgg
      961 cagcgcgcat gaggtggcgc tggtgcgggc ggcgatcgag cagggtggac tggaccattt
     1021 cgatgacatc cttgcggcga tccattccgg cggcgcgctc gaatacacac gcgagcgtgc
     1081 ccggcaggaa cgcgcactgg cgcaagctgc actggccggg ttgcccgact cgtcctaccg
     1141 gcaacacctg tccgaactgg ccgggtttgc ggtcagccgg acgtattgat ccattctgtt
     1201 tgctcggtga ggtgaacgga ctttgcctga aacgatcctg cgatgctgaa actgctggtg
     1261 cttggactgg cctcagtcta tctgtggcgc cgctggcatg ccagcccgtc cgctggggag
     1321 gtcgtgccgc cgcgtaccgg aggcgcgcat cgcaccatcg atacggtgcg ttgtgcgcat
     1381 tgcggcatgc atgtgccgcg cgatgaggct gtggggggcg gcgagctgtg gttctgcagc
     1441 gcagcgcatg aacgggagtt ccggcgcaat ggcggctgac cgaggcgcgc tgtccggcgc
     1501 ggtggaagcc gtttcgccgt cagcgcgcat cgagcgcaac tggaattcgc tgctctacct
     1561 caacctctac cgcttcatgg tggcgaccgt gctgttgtgg ggcagtctgc tggcgccacg
     1621 ctggcgcatg tttggccagg acaaccctgc attgttcctt gcctgcgccc tgggctacga
     1681 gatgctggcg ttgctgtcca tggccaccat cacccggcgc tggccgcgtt tcttctggca
     1741 gctcaatttc cagattgccg gcgacatcct ggcgatcacc gtcatgcttg cggtgagtgg
     1801 cggggtgcgc agcgggctcg gtctgctgct tatcgtttcg ctggcctcca gtgcgctggt
     1861 gaccgaaggt cgcatggtga tgttctattc ggcgctggcc accagcggca ttctggtcag
     1921 ccaggcgttc agcctgtgga gtggagagcg cggcatcacc gatatcggcc aggcgggatt
     1981 gctcggtctg gtgtttttcg ccaccgcagt ggtggcgtgg gtgctggtcg accgcagccg
     2041 catgaacgaa gaactggcag cggcgcgtgc catcgatctg cacaacatgg cgcagatcaa
     2101 ccagctggtc atcgacgaca tgcaggatgg cgtggtggtg ctcgacggcg aaggccgggt
     2161 acgtcaggcc aaccagcgtg cgatcgaact gatcggcagc gtgaccgatg tccggagcgg
     2221 ccagccgctg gcgctggaac agtgcgtgcc gttgctgtcg gaatacctcg aacagtggcg
     2281 gcgcagccag ccgctggtgt tcccggtggt ggcgctgggc ggacagggtg ggctgtatca
     2341 gccacgattc atcgcggtga ccaaccggcg cgccatgggg gcggtgctgg tgctcgaaga
     2401 catgacgcgt gcgcaggagc aggcgcaaca gatcaagctg gcggcgctgg gccggctgac
     2461 ggccaatatc gcgcacgaga tccgcaaccc gcttgccgca atcacgcatg cgtcggaact
     2521 gatgcgggaa atgtgcgatg atgccgcgct ggaccggttg gtgcagatca tgctcaacaa
     2581 tggccggcgc atcaatgatc tgatcaacga cgtgatggcg ctcaaccggc gtgaccgcat
     2641 gcaggtggaa cagatcgaac tcaaccgttt catgcttgaa tttgttgacg aattccggca
     2701 tacccagcaa cttgcggacg aggtgttcga tgtgcagatt cccgacagtc tgctgatctg
     2761 tttcgaccgg cagcacctgc gtcaggtcgt gaccaacctg tgtctcaatg cggtcaggca
     2821 cggcagccgc caggcgggca gcattacgat acgcgcggaa tcggtgcgca gccagatgat
     2881 gctgcacatc gtggatgacg ggccgggcgt ggcggcggaa atcctgccgc ggttgtttga
     2941 gccgttcttt accaccgaat cgtccggcac cggtctgggg ctgtatattt cgcgtgaact
     3001 gtgtcaggcc aacggcgcca gcctggaata tgtttccgcg ccgaccggtg gacattttct
     3061 catcaccatt caggaggggg catgtcaaaa cgagccgtga acgggcagtt gcctgccgtg
     3121 ctgatcatcg acgacgaggc cgatatcctg gaactgctcg agatcacact ggtgcgcatg
     3181 ggtctggacg tcggttgtgc tggcagtgtg gccgaggcca aggcgcatct tgcggagcgg
     3241 aactacgacc tgtgcctgac cgacatgcgt ctgcccgatg gcgatgggtt gcagatcgtg
     3301 cgtcatatcc agagcgaata tccgaccgtg ccggtggcgg tcatcactgc gcacggcaat
     3361 accgacaatg ccgtgaccgc gctcaaggcc ggcgcgttcg attacatcaa caagccggtg
     3421 acgctcgacc agttgcgtgc cctggtcaaa tcggccctgc gactgccggg gccccgcgtc
     3481 tcggtgggcc gggagctggt cggcgactcg cctgccatgc gtcaggcacg ggagtggatc
     3541 gccaagcttg cgcgcaccca ggcaccggtt tatatcaccg gcgagtcggg cagtggcaaa
     3601 gaactggctg cacggctcat ccatcagcag ggcagccggt cggtggcgcc attcattccg
     3661 gtcaactgcg gtgccattcc ggaaaatctc atggagtcgg aatttttcgg ctacaaaaag
     3721 ggggcgttta ccggcgcgga tgtcgaacgt gacggatttt ttcaggccgc atcaggcgga
     3781 acgctgttcc tcgatgaggt cgccgacctg ccactggcca tgcaggtcaa gctgctgcgc
     3841 gccatccagg aaaaaagggt gcgcaagctg ggtgcgccca acgaagagcc gatcgatgtg
     3901 cgcatcatca gtgcgacgca ccagaacctg gcccagtgtg tcgaaagcgg ccgcttccgc
     3961 caggatctgt tctatcggct taacgtcatt acactgaaaa tgccttcgtt gcgtgacatg
     4021 cgcgaagata ttccattcat cgcgcagaat ctgttgcatg gtatggccga tggggagcgc
     4081 atccctgtgc cgcaactggc ggccagcgcc ctgaccgcgc tggcgcgcta cgattatccg
     4141 ggcaacgtgc gcgagctgga aaacattctc gaacgcgctc tggcgctcag ttccggcaac
     4201 cagatcgagg cggatgacct gcagctcact ccggcggaaa tgttcgctga cgaggtcgtt
     4261 gccggtacgg aggtgggcag cggtgcgaaa tggccgctgc aggaatatct cgaccggctc
     4321 gagcgtgaag cgattcagga agcgctggaa aaaacacgtt acaacaagac tgccgcggcc
     4381 aagctgctgg gcatcacctt ccgcgcgttg cggtaccggc tggaacgact gaacatggag
     4441 tgagcacgct ggtgcaccac tgtcgggtat ctgtacatgg ctgttgcaga tcgaggcggg
     4501 tcattgtctg aagcggtaac ccagaccgct ttcagtgagc aggtatgtcg gctgcgcggg
     4561 gtcgtgttcg agtttcctgc gcaggttgct catgtagatg cgcaagtagt gcgggctgtc
     4621 cacgtggctt gggccccaca cttcgcgcag cagttcacgg tgtgtcagta ccttgcctgg
     4681 atgggcgagc agataagcca gcaggcggta ttcggtcggt gtcaggtgga catcggtccc
     4741 gccacggctg gcagtgcgat tggcgaggtc gatgcgcaca tcgccgaaat gcgtttcagg
     4801 cagactgttg tcgtttgaca tgtgcctgcg gcgcaactgg gcacgtacac gcgcttgcag
     4861 ttcgggcatg ccgaaaggct tggtgaggta atcatcagcg cccgcatcca gcgcggcgat
     4921 cttgatgctt tcgttggatc gtgctgacag tacgatgatc ggcaccgatg accagcttcg
     4981 caggtcgcgg atgaattcga taccgtcccg gtcgggcagg cccaggtcca gcaccacgag
     5041 atcgggtttg cgcgttccag cttcggtcag tccgcgtccg gcggtttcgg cctcaaatat
     5101 ctggcagccc gcagattcaa gcgccaggcg aacgaagcgg cgaatctgtt tatcgtcctc
     5161 gaccaggatc acgacgggcc cgctcatgtg tcctccggcg gtttgaggtt gggaggggaa
     5221 cccagtggca gggaaaacgt gaaacacgcg ccatgcgggg cgacgttttt cgcatggatg
     5281 gtgccaccgt gtgcttcaat gatagcacgg cagatggcga gccccagccc gatgccgggt
     5341 atgggcgatt ccggtcgacc gcgagtgaat ttttcgaaca gctgttcttc ccggccaggc
     5401 ggcaacccgg ggccctggtc gcataccgag atttccatca tggaggactg cgtagctgcg
     5461 cggatggtga taggggtccc tgcgggcgta tattttgccg cattttccag caaattgacc
     5521 agaacccgtt cgatcagcac gctgtcgaat tccacgaagg gcaggtcgtc gggcagctcg
     5581 acctgtatgc tgcgccggtt cagtgcagtc gtcgaggcat ggatggcgct gccgatcact
     5641 tcttcgatgg gttgccattc ccgtttgagc atgacgttgc cggtctgcag tcgtgccatg
     5701 tcgagcagat tgttgaccag ggcgcccata tggatggctt catcgtgaat cgcctgtacg
     5761 gtttcctgtt ggcgaggcgt gagcggttgc aaggtcagag tgtcggacag gccgaccagt
     5821 gcggtcagcg gcgtacgcaa gtcatgcgac aataccgaca gtaccgaatt gcgcagccgt
     5881 tcggattcca ttctgaccaa tgcgtcctgt gccacttcca catagtggac gcgttcgatg
     5941 gcgatggcca ccagggtgga aaacgtgtcc agcagtcggg tgtgctccgg cagttgtaac
     6001 cagtcgctct ggtgcggctg aatggccagc acaccgcgga tctgcatggg tgccttgagc
     6061 ggcaggtaat acacgggtga ggctggtagg gtatcggtgc caagaccggc ggtctcgccg
     6121 tggtcgaacg cccactgggc gatggccaca tcgacctgga tttcgttaat gtcgtgtgtt
     6181 gcatcgagat gattatgcgc gtctggcagc aggattgctg cgtgtgcatc gaatacgcgt
     6241 gtgacaaagc ggtcgcaaat ctcggtgatc tgctgcgtca gcagtgctgc cgacaggtca
     6301 cgtgcggctt cgtagagtgc gcgcgcccgc atttcgcgac tcatggaaac cctgacctga
     6361 tagcgcaggt tggccatcag ctgcgcaatt accagtgcga cagccagcat gacgataaaa
     6421 gtgaccagat attgcacatc gcttaccgcg aagg
//
LOCUS       DAIANL010000137         4451 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_586899, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000137 DAIANL010000000
VERSION     DAIANL010000137.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586899"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          858 a         1204 c         1434 g          955 t
ORIGIN      
        1 tcgcaacccg gtgccgccta ccgcgcatcc ctcagccgtc ccttccgtcc cgcaaccgcg
       61 ccagcgtatc aatcggcgca ggctggatgg cccgcagcac aacactttac cggtccggat
      121 acctccgatt gctggatgag cgtaaccgtc cgacgcaggc atctaccgaa acggtgattt
      181 cccgccaata ctagtcccca cttattttac gtggggactt atcaatggtt tcagccgtca
      241 ttcccattcg ccatcgccga cgtttcagtc gggaattcaa acgccagctg gtggatcaat
      301 gcacacccgg tgtcccgttt gcgggaattg ctttatcgca tggcattagc gccaatctgt
      361 tgcgtcgtcg gatcgggaaa ttccgggttg atgtcaactt gacaggctgg tacggccagc
      421 acatcgaccg agatacgggt tctgctgtcc gtgagccatt tccagccatg aaatctctgc
      481 cacaagatcg aatccggggt atgttcttca tcggcgaggc agcgcgttgc accctctgga
      541 ctgcttcggg agtacaattt ttctgccggt ctgacttgag gaggtgatga atgtctggct
      601 accgctatat tcgttttttt ttacaggttg gtatcgacga cgtgtcgctg gtgggtggga
      661 agaatgcctc gctgggggaa atgacgcgcc acctgaccgg ggaaggcatt cgcgtgccaa
      721 acggttttgc ggttacagct gacgcgtacc gcttcatgct cgatcaggcg ggcgcgtggg
      781 atgcgttgca cgcggccctg gatggcctgg atccgtctga tgtcagcgat ctggagcggc
      841 gcggcaagca ggcgcgcgag attgtatacg gcgccggatt gccccaggcg ttggcgcagg
      901 acattctcgg gggctaccgt gagttgcgcg agcaatatgg cgaagatgca agtttcgccg
      961 tacgtagttc ggctacggca gaagatctgc caggggcaag ttttgccggg cagcaggaaa
     1021 cctttctcaa tgtgcgcggc gagcagagcc tgcttgatgc ttgccgacgc tgttttgcca
     1081 gtctgttcac tgatcgggcg atacactatc ggatcgatca ggggtttgac cattttcgcg
     1141 tcgcgctgtc gatcggtgtg atgaaaatgg tgcgctcgga tctggcgtcg tcaggcgtga
     1201 tgttcacact ggacacagag tcgggctttc gtgacgtcgt cttcattacc ggggcgtggg
     1261 gcctgggtga gaatgtggta cagggcgcag tggatccgga cgagttttac gtacacaaac
     1321 cgggctatct ggctgggcac cgtgccgtgt tgcgccggaa catgggcgac aaggcagtga
     1381 aaatggtata tgtggaaggc gagacacggc atgtgacgcg caatgtgccg acaccgcgtg
     1441 ccgatcgcga gcggttctgc ctgagcgata acgaagtgct cgaactggct ggacatgcgc
     1501 tgaccatcga gcggcattat ggtcggccca tggacattga atgggcgaag gatggtgtgg
     1561 acggtggact gtatatcgtg caggcccgac ccgagacggt ggctgcacag cgcagcgcaa
     1621 ccgggttgcg taatttcgtg ctcgacggcc gcggtgcggt gctggtgacc ggtcgcgcgg
     1681 taggcgaggc gattgctgcc ggctcggcgc gagtgattgc cgatgtggca cacctggcgg
     1741 catttcgtcc tggcgaagtg ctggtggcgg acattaccac accggactgg gaacccgtga
     1801 tgaaaaccgc agccgcaatt gtcaccaacc gcggtgggcg cacctgccac gcggcgatca
     1861 ttgcacgcga actggggatt cctgccgtgg tgggtgccgg cgatgccacg tcagcgatcc
     1921 agaatggcgc accggtaacg gtctcctgcg ccgagggcga cacggggcat gtgtacggtg
     1981 ggcagattgc ctatcatgtg gatacgatcg aaattgccga gatggcgcgg ccggtaactg
     2041 ccatcatgat caaccttggc aatccggaca tcgccttccg cacgtcgttt ctgcctaacg
     2101 atggtgtggg attggctcgc atggaattca tcatcagcga atccattcgt gctcatccgc
     2161 tggcgctgct gtatcctcaa cgcgtgacca gcgagcatga gcgcacgcaa atcatgcgca
     2221 tgctgcgtgg ctatgccgat ggcgaatcgt tttttgtgca acgcttgtcg gagggaatcg
     2281 gcaccattgc cgcggcgttc tggccgaagc cggtggtggt gcgcatgtcg gatttcaagt
     2341 cgaacgagta cgccagtctg ctgggagggg ccggattcga acccacggaa gccaatccga
     2401 tgctcggttt ccgtggcgca tcgaggtatg cacatccagc ttatgccgag ggtttccggc
     2461 tggaatgcct ggccatgcgg cgcgtgcgtg atgacatggg cctgaccaac gtaatactca
     2521 tgttgccatt cgtgcgacgg gtacaggaag ccgatcaggt gctggaacgc atggcgcaat
     2581 acggcttgca gcgcggacaa aatggtctgg aagtgtatgc catgtgcgag attccaaata
     2641 atgtcattct catcgaccaa tttgccaccc gtttcgacgg attttccatc ggatcaaatg
     2701 atcttacgca actgacgttg ggagtggacc gcgacagcga aatcgtcgct ttcgattatg
     2761 atgagcgtga tgaaggcgtc aaagcgatgg tgcgactggc ggtcgaaggc tgtcgccgta
     2821 acggcattca ttccggattg tgcggccagg caccgtccga ctatcccgag atggcggcct
     2881 ggctggtcga gatcggcatc gacagcatga gcctcaatcc ggacaccgtt ctggccacca
     2941 cccgtcatgt gcttcaagtg gaacgagagt tgggtcggca caggcccggc actgaatgag
     3001 taacatgata agtcctgcac caacagggcg gtgccgtgac ggggtatggt atcatggccg
     3061 ccgtttgtcc cggttgggga cggtgtgtac gtttgcggca ggtgagtcat gaaagaacat
     3121 atccagagat tcaattccta tgcgctatcg gtgatcgaac acgtcggact gatggtgatt
     3181 tccgtggcca ccgtggtgtc catggccagc gagatcctca agatgtatga ccgtctcgaa
     3241 gtgacgctgt ctgacctgct gatgctgttc ctctacctcg aagtgatcgc catggtgcag
     3301 cgctattaca cttcgggcaa gatgccggtg cgctttccgc tgtacatcgc catagtcgcc
     3361 ctggccagat acatgattct ggacatgaaa ctgatgaccg aatggcgcat gatggccatc
     3421 gccgcggcaa tcctcattct ggcgctggcg attctgctca ttcgttatgg gcatgtgcgt
     3481 tttccctact ccagtgacga aaacctgcag cgtagcgaac atcgcgaaga acgcgactga
     3541 ctgtgtggct aggcgcattg ggaacgcagc gtggtcagca ggtaacgtgg aagatgcgcg
     3601 gggcgtggta acccggattt cggttcagac ggatacgtga ggaaacggca atgcaactgc
     3661 cgtcccgcat cagttccagg agtgtcgacg tcgggagtgc tggatgctca ggcgggttcg
     3721 atgcgcagat tgcgcggcag gccgatgatt tttcgtccca cacagagcag gatgtcaatt
     3781 tcccgcccac tgcggttctg gttggcacgc cactcggtca gggtgcgctg atcaatgggc
     3841 agcatgattg gctgccgcgc tgcagcgccg gctccggaga ggtaaaacgg ttgctgccgg
     3901 gggaattcgc cggtttcgag aatcagtgtc gcactcgatc cgctgctgtt caaagcatcg
     3961 gggcttgcgg ggccggtcag tggcggctgg tgcacggcat ggatgaagtc agcctggtcg
     4021 agcgcgctgc ggcttgcagc gcaatggtat tcgagccaca gccaggcggc aggactcgga
     4081 gaaacgaggt gcacgagcgt gctggcgtcg gccagggtaa gcaggatggc cgcgccagcg
     4141 ggcgacagcg gtgccggggc ctgcagacca ttctggattt ccacggcccg tcccggttca
     4201 ctcattgccc gtaaaatagt gcggtagcag cgctgcgtat cgcgaaccgg gtcggaaaaa
     4261 ccccgggcga acaggttgcc acgcatgagc gcgtgattcg aggccggttg attcatgttc
     4321 cacactcccc cctgtacggc gacaccatga tcgaaccatt ttcgccatgg ggctggcgaa
     4381 cgtggaagtc gcggaaagcg cggcgtaccg tcgtgcacaa tccccgtcgt gtcggcgatt
     4441 gcgttgggca t
//
LOCUS       DAIANL010000138         4417 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3033530, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000138 DAIANL010000000
VERSION     DAIANL010000138.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3033530"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          813 a         1237 c         1545 g          822 t
ORIGIN      
        1 gccgtgccca ggccgaacag ggcgccgggc agccagcccc aggcggcatt cggcatggtg
       61 ggtgcaagtg tcgtgtacag gatgagcgca aatgcaccga aaccccagcc ggcgacgaaa
      121 ccgtgaatgg ccggcatgcc gcggatcacg cgggcaagcc caggacgcga tcctggttgg
      181 gtgctgtgta ccatcagcca gccaccggtg gccatcagca gcccgatcgg gatcagcaac
      241 aggcgttcga gccagggatt ctgcatccag tgcgacaggc cgaaccaggc cagttcgcag
      301 gcgatggcgc gctggatggt gaaactcagc gaaaacagca ggccggcgcg cagaccgccg
      361 cgcgaggaat agctgccgac cgcatagctg aaggtgatcg gccaggtgtg ttcatcgggt
      421 atcaggccgt ggatcaggcc gagcagccag gccgtgacca gtgctgcaat gactccggaa
      481 tgggccgtcg ccggagccca ccagtcggtc agatgcatgc gtaacggacg caacgggttg
      541 tgcgccgggc aagacggtgg atcagggcag ccgggcggcc aggtgcggga atgcacaggg
      601 attccgactg catccgtgca gggccgtggt gcagatcgcc tgccatgctg cctgtacacc
      661 ggatatgttt caatgtgttc gctccatgca cctcattcca tggcgtgttg ctcgccgaag
      721 gaccccgcgt tctcatcatg tcattcgatc cgtttgagct gttccggctg tagcaggcga
      781 atggcggacg catccacgtg gatcagtccg tcttcgatga agcgcgcgag cagccgggac
      841 agggtttccg gacgtatgtc cagcagcgaa gcgatggcct gcttgctggc cggcaattgt
      901 atcacgcgtc cggtctgctg caggattccg ggctgcgcga gcaggtattg cgccagcctg
      961 cgcgcaccct gttgcagtgt gatgcggtcg atatcctgaa ccaggccatg cagttgccgg
     1021 ctcatggagg ccagcaggtt gaggcacagg cgtggacgtt cgagcagcag gccatggaag
     1081 aaggccgagt cgaaggcgat cgggcgcgac gtttcgagtg cctgggcatg caccggatag
     1141 ctgccgccca tgaacatgac cgcttcggcg aatatctgtt cgcggccgat gagttcgagc
     1201 gtgtgctcct gaccgttttc ggacagcagg tacaggcgta ctgcgccgga tttgagcaga
     1261 aagaaagacc gcccctcctc gccctgagag aacagcggtt cagccgcttc gagcatgcgc
     1321 cagcgggaat gcagcgccag ttgcgccaga acatcgcgat caaggtcgcg aaacaggtgc
     1381 gccagccgaa actggctgac atcgggatgg ggactcacgg ccatttgatc caggtcaagg
     1441 caaatattgc gcggcagatg caaactgcaa ccatcgtaac acacaggaga attgccatgc
     1501 acaaacgcaa tcgatggttc gtcagcatcg ggctggcgta tggattgctc ggcgctgcca
     1561 ttgccctggt cttcttgtcc gatgccacgt ttatccctgg taatgtgcct cgcctgcacg
     1621 ggcacatcat gctgctcggg ttcatgctga tggtgattta tggcattgcc ctgcatgtgc
     1681 tgccgcgctt ttccggacag ccgctgtatt cggaacgcat ggccgactgg cagctgtatc
     1741 tggccaatgc gggattaccg ctgatgattg caggctggtt gagcctgcgt aactggctgc
     1801 tgcttgcggg aggcagcctg caatttgctg cgatcgtact gttcgggttg aatattctgc
     1861 tcacggtacg gccgcaaggt ccgtggaggt gaatcatgga atatcgtgga tgtctgctgc
     1921 cggcagatct gtattatgac cttgattaca tctgggtgcg tccggaagat gacgggttgt
     1981 ggaccatcgg actgacggac ccgtcgcaga ccatggccgg caaggtgcag tacgtgtgga
     2041 tcaaggagcc cggcacctgg cgcaagcaca agaaaccgtt ggcacgcatc gagtcgggta
     2101 aatgggcagg cgggataccg gcgccgtgcg atggcaccgt cgtcgagcgc aacgaacgcg
     2161 tgctgcaaca gcccaacctg atcaatatcg atccgtacaa cgatgcctgg ctggtgcgct
     2221 ggaaaccgga tgacccgcag gccggtctgg ccgcactgtc taccggacgt gtggcgcaag
     2281 aggcgctgcg ccagtggatc gaccgctaca acgtgcaatg catgcgttgc caggaatgag
     2341 ggagatgatg cgatgaaagt ggaattgctg gtgtcggaat ggtgttcggc gtgcaagcag
     2401 gcggaacagg tgtggcagca ggtggccgca gagaaggaaa tcgagtttgc cgtgctggac
     2461 atggcgcagc cggaagggcg cgaactggtc agccggctgc ggctgaaaac cgtgcctgcg
     2521 ctggtgatcg atggcgagtt gcgcggcgct ggcgtgcaga ccccggcgca ggcgcgcgag
     2581 tggacggcat cggctccgcc acggcgccag gccgcagtgc agaatgcagg attgctgctg
     2641 tcgctggaca accggctgtt cattctgggc gcggtactct gcctcatggc tggcggatcg
     2701 gggctggtga tcaacggcag tctgctgtcg gacggcccgg ctcgtccggt ggccatgcat
     2761 ctggtcacca tcgggttcat gctgatgttg ttgtacgggc ttggtgcgca catgctgccg
     2821 cgcttcatgt ccaggccgat ccgcaatggg gtctggccct ggctgcagat ggggctggtc
     2881 caactcggac tattgggcta tgtggtcggt ttctgggccg cgcgtcctgc gctggtgatc
     2941 gcaggcgggg ggctggtctg gctgtcgctg gcgattttca cctggcggct ctggccagtg
     3001 ttgtggtttg cgccagacac gacggagacg cggccgggac attcctgagc ccggtttcag
     3061 tctgtgctca tggcctcgcc gaggcggacg gcacggcctt ctcgccagtc gtgtggaaag
     3121 gcaggaccac cagacggacg cagcaggatg accgtcgagc cgagctggaa gcggcccatt
     3181 tcttcgcccg gcgccagtgc aatgtcgtgc gcgtcatagc gccattcgcg cacgcggtgc
     3241 gcgcgcggcg ggttgaccgt gccatgccag atggtcgcca tgctgccgac gatggtggca
     3301 ccgaccagca ccaggatgaa cggtccgtcg gtggaatcga actcgcagac caggcgttca
     3361 ttgcgggcga acagtccggg aatgtggcgt gcgctggccg gattgacgga aaacagcgtg
     3421 ccgggaacat gcagcatgcg gcgcaggtgg ccggcatgcg gcatgtgcac gcggtggtaa
     3481 tccttcgggc tcagatacag cgtgcagaac gatccgtcgt acacctgtgc ggccagagcg
     3541 gcgtcgccgc ccagcagggc cagcgtcgaa tatgtctggc ccttggcctg gatcatctgg
     3601 ttgccgtcga tgcggccggc ctggctgacg gtgccgtcga ccgggcagat ccagcgactg
     3661 tcggccagaa cgcgtgcgcc cggacgcagc ggacgggtga aaaattcgtt gaagctgcga
     3721 tacccctgcg ggctgtctgg ttgcgcttcg gtcaggtcga ccgcatagcg tgccagaaag
     3781 cgcatgatca gccattgcgc gagtggacgg taggtgctgg cggcaagtcg gccggcaaga
     3841 cgggtcagca gcagggcggg cagcagggat tgcagtcggg tggcaaggcg ctcgggcacg
     3901 gaaggctctg caggacaaag gcggcattat agcaaatcgt ccgcgtgcgt agtccgctcc
     3961 gccgcatatg gcgtcgggca tcgccagtcg atgcatggtg tgtcatgtct ggttgtgcgt
     4021 gctggtgcag cggctccggc ctgctgatga cacacccggg atgggttaga atggaacggt
     4081 cattggtaca gcggagtgca cggcatgact gacggcgcga gtcgtttcaa aggacgtttc
     4141 ctgcttgaaa aagatggcga gtcctggctg ggcggacagc gcatcgattt gctggaagcg
     4201 atcggcgtgc acggttcgat cagtcaggcg gcacggatgg tcggagtcag ttatcgcggc
     4261 gcatgggata ccgtggatgc gatgaacaat cagtccgatc tgccgctggt cgaacgcgtg
     4321 gcgggcggca aacacggtgg cggcacgcgc ttgaccgagc atgggcgacg cgtgatccgg
     4381 ctgtttcgtg ccatggaagc cgggtatcag cgcgcgt
//
LOCUS       DAIANL010000139         4115 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2425415, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000139 DAIANL010000000
VERSION     DAIANL010000139.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2425415"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          720 a         1200 c         1421 g          774 t
ORIGIN      
        1 cgctgaacag caaccggtca aatcggtgcg cggtcacggt taccagttgt gcgtcaaggt
       61 gcatgtggcc tgagcgatat accccggacc tgctgcggaa gcggcaaggg catcccatgc
      121 gcggcggtct gaccgcagtc tgcagacggc cgtgaccgtg accggtaccg gccggagcgt
      181 ttctgttccg gcgttgcatg gacgctaccg gatgacgccg ggcgacgccc cgcagtcgat
      241 gcccgatgcg gcggacagca tgtggcgaac ttgcgtgagc ggggcagatt gcgtgacaat
      301 gcccgcattg gggcagagtt gcccgcggtc tgaagagtgg aatacatggc gttcaatttc
      361 gccgacaagc tggtggtggc gatttcttcc cgggccttgt ttgatctcga cgccagtcat
      421 gccattttcg agcaggacgg cgtggaggct tattgccgct atcagatcga gcatgaagaa
      481 gacgtgctgc aaccaggtat cgcctttccg ctggtacgca agctgctggc gctcaaccag
      541 gggtttcccg aggcaccgct ggtagagatc attctgttgt cgcgcaacag tgccgatacc
      601 ggattgcgta tcttcaactc gattcgtcac catcagctcg acatcacccg tgcggcgttc
      661 acccgcggtg agagtaccca ccgttacgtg gcggcattcg gcgcacacct gtttctgtcg
      721 gccaatcctg acgacgtcaa gagcgcgctg gcagcgggtt atgccgcggc aaccatcctg
      781 ccgtccaatg cggggcaaaa cgaggggtct tcgcagctgc gcatcgcctt tgacggcgat
      841 gccgtgctgt ttgccgacga ttccgagcgc atctaccgca caggcggcct ggaggcattt
      901 gagcgcaacg aacgcgatgc ggcgatgcaa ccgctgagcg gtggaccatt caagggtttc
      961 ctgtccgcgc tgcaccgtat ccagtcgatc tatcccgaag gcgcggcgcc gattcgtacc
     1021 gcgctggtga cggcgcgttc tgcgccggcg catgaacggg tgatacgtac catgcgcgca
     1081 tggaatatcc gcatcgacga ggcgttgttt ctgggcgggc tggacaaggg cgaatttctc
     1141 aaggccttcg gtgccgacat tttttttgac gaccaaaagg gtcactgcga ttcggccagc
     1201 cgccatgtgg cgaccggaca tgtgccgcac ggcgtggcca acgagtgatc cgtcaggcgt
     1261 tctggaagcg caggcggcgg aagatggctg tcttgaccac atccaggacc agcagccaga
     1321 cgatgatcgt tgccagcagc tcggccaccc gcgctggcgc aatggccgtc atcaggatgc
     1381 cgtgcgtggc gagcaggctg actagcgcga tgtcgagcag tgaggtgcgc aacagccatt
     1441 tccctggtcg cgattgccag aacgggccgc gcacgcgtac caggtaaatg gtgccctgcc
     1501 cgctgaatac cagcatcagg aatatcagtg tctgcaactg cggtagcggc aggtgcagca
     1561 cattgcgcgc aaaaaagaac acgctgaatg acagtaccag catgccgccc gcgagcaggc
     1621 cggcgatacc catcagtgca ggcacattcc agcgttcggg ccggtcggat ccctgggcgc
     1681 ggtcgctggc gatggacatg gtggcgaagt cgttggtgaa cagcagcagc acgatcagca
     1741 tcggtgtgat gacaaatgtg ccggtcagca tcagtccaac gctgagaaac agggcgattt
     1801 ccagtgtctt gatgatcttg ttgagcgtgt aggtcagcat gcgctggtag atgcgccggc
     1861 tggtttccac ggcgttgcag acatcggaca ggccggggct ggtgagcacc aggctggctg
     1921 ccgctttggc gacgtcggtg gcggaggcca cggcgatgcc gacttctgcc tgtttgagcg
     1981 ccggggcgtc gttgacgccg tctccgctca ttccggtgat gtggccgtcg tgctgcagcg
     2041 catgcaccag atggaatttg tcttccggca gcacacgggc aaagatgtcg taatcgaggg
     2101 ggtgattcat gccgtcctgg acggtgtcgg ccgggcagac gcgctcgccc aggccaacct
     2161 ggcgtgcgat ggcgcgagcc gtggctgcgc tgtcgccact gaccatgatc acgcggacgc
     2221 cgagcttgtc aagtcgcgcg atcagggcgg cggaatcctc gcgcggcggg tcgctcagcc
     2281 cgaccaggcc ggcgagctgc agcgcatcgc cttcgccgac tgccacggcg agcacgcgca
     2341 tgccctgttc gcccagcctg tccaccattg tctggacatc aggtgccccg ggaacaagcg
     2401 cagcgacggc gtgcggcgcg cccttgatga cgtgcatgtg tcgggtaccg tccatgaaac
     2461 gggcttcgga ccgcttggtg tccggcgcaa acgggatgaa ctgcagtcgc tcgggcacgg
     2521 ggacatcggg ttgtgctgcc cggtcggcag tcaggatggc cagatcgagc gggtcctggg
     2581 tggccgggtc gctggcgatg gcggcccagc gcagcaggct gaccgcgtcg gcaggcgcga
     2641 acggcagtgt ggccgcgacg ctgagctggt tgcgcgtgat ggtgccggtc ttgtcgctgg
     2701 ccagcacgtc catggcggca gcatcctcaa tggccgacag acgcgtgacc agcacaccgt
     2761 gcgcggccag ttcgagcgcg ccgagcgccg tggccagggt gtaggttgcg ggcagggcga
     2821 tcgggatcga ggcgatcagc aggatcagtg aaaacggcac gatttccgtc agcggcttgc
     2881 ctgtgctcag tccatagaca atcatggcag cgatgagcat ggcgtcgagc acaatcaggt
     2941 aacgcacgat gctgaaaatc agttcttcca ggtggctgac cgggcgggcg gtatcgatca
     3001 gtccggcggt cttgccaaac tgcgtccggg caccggtggc gctgaccgtg ccgctggctt
     3061 caccgcggcg gacggtcgtg ccggcaaacg ccttgtcggt gccgtgcagc gtgaccggga
     3121 gtgattcgcc ggtcagcaca gactggtcga gcaggatttc gccatcggcc agttgcaggt
     3181 cggccggcac gatatcgccc atgcgcaggt ggatggcgtc gccgggcacc agctgcggtg
     3241 ccgggagcga ttgccaggtg ccgtcgcgca gtacgcgcgc gcggatttcc agcttcgacc
     3301 tgagcagttc gagtgcgcgg ttggcacgtt gttcctggct gaaaccaagg atggcgttga
     3361 agccgagcag gacggccatg acgatcgcct cgtcgggctt gccgagcacc agttgcagca
     3421 cgatggcggc ttcgagcatc cacgggacgg gagaccagaa tttgcgcagc aggatcagcc
     3481 aggccgggtg tcggttcgca ccgacctggt tcggtccgta ctggcgcagc cgccgagcgg
     3541 cttcttcgct gctcagcccc tgcagggaag aatgaacggg catggacatc cccagctggt
     3601 tttgggccca gtctagcatg gacggattga cgtgcggctg actgtatgca atgccaggat
     3661 ccgtttcgtg tggccagacg gcgagccggg cggatgcaac accggcggca tcggtgccac
     3721 agttttgtcg ggaaattgtt ttatactgga aaagtcgcgg gtgcggaaat ggatgatggg
     3781 gttcagacaa gggatattgc agcgtttcct gtttcggtca ctggtggtct ggctggcggg
     3841 ggttctggtg gtcggactgg tggcctggat gtcctggcag gaggccaccc ggcaggccga
     3901 gaagatccgc ggcgtcaatg tgcagcatta ctttcgtgcc cgcatcgacc agatcggtaa
     3961 tgacttcggc cgcgacgcga cacgcaacgc catgtatctt ggctcaaggc tggctgcgga
     4021 gggggggctg cccgtctgcc aggccctgca ggcctattat gatgcgctgg gtggtgtgct
     4081 gccatttggc atggtgcgcc tgctcgatgc gggcg
//
LOCUS       DAIANL010000140         8773 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2010156, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000140 DAIANL010000000
VERSION     DAIANL010000140.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8773)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010156"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1548 a         2475 c         2913 g         1837 t
ORIGIN      
        1 agccgctggc gatctttccg cacggacgca tcacagcgtt gctgcgcgat ctgccgcccg
       61 aacaagcatt ttgcatccac tggctggctt attatccggt gctcgagcag gatgggagcg
      121 tgcgtaaatc caccggcaca cataccccgc acctgcgccg gctggaggcc gagagcatcc
      181 atattctgcg cgaggcggta gccgaagcgg aaaatcctgc catgctgtat tcgatcggca
      241 aggacagctc ggtcatgctg catctggcac gcaaggcctt ctatcccgca ccgccgccgt
      301 tcccgttgct gcacgtggac acacgctgga aattccgcga aatgtatctg ttgcgcgact
      361 ggatggcgcg tgagagcggc atggaactgc tggtccatgt caatccggaa gccatcgaaa
      421 agaacatcaa tccgttcgat cacggttccg cactgcacac tgacatcacc aagaccgagg
      481 ggctgaaaca ggcgctggat aaataccgtt tcgacgtggt gtttggcggc gcacggcgtg
      541 acgaggagaa atcgcgcgcc aaggaacgca ttttctcctt ccgcacccag agccatcgtt
      601 gggatccgaa gaatcagcgc cccgaactgt gggacctgta caacacccgc atcaacaagg
      661 gcgaaagcat tcgcgtgttt ccgctctcga actggaccga actcgacatc tggcaataca
      721 tccatcagga aaacattccg gtggtgccgc tgtatttcgc caaaccgcgc ccggtggtgg
      781 tgcgtgacgg catgatcatc atggtggacg acgaacgcat gcgactgctg ccgggagagc
      841 agatcgagct gagaacggtg cgtttccgca ccctgggctg ttatccgctc accggagcga
      901 tcgagtcgga agcgcgtaac gtcgaggaca tcattctgga gctgctgcag gcccgcagtt
      961 cggaacgtca gggccgtgcc atcgactccg attcgggcgc cagcatggaa cgcagaaagc
     1021 aggaagggta cttctgatgg cacgcaaatc cgcaaaccgt tcctcgaccg gcgtgaccgc
     1081 agatgtgccg cacagcatgg aagcattcct gcgtcagcag cagcggcagg acctgctgcg
     1141 tttcatcacc tgcggcagcg tcgacgatgg caagagcagc ctgatcggcc ggctgctgtg
     1201 ggaatcgcac cagctgttcg acgaccagct cgcagcactg caggctgaat cgaaacgcca
     1261 tggcacgcaa ggtgacaaca tcgattttgc attgctggtg gacgggctga ccgccgagcg
     1321 cgagcagggg attaccattg acgtggcata tcgattcttt gccgtggcca cgcgcaaatt
     1381 catcgtggct gatacgccag gccacgaaca gtatacgcgc aacatgatta ccggcgcctc
     1441 gaatgccgac cttgcggtgt tgctggtcga tgcccgtcag ggcacgctgg tgcagacacg
     1501 gcggcatgcc tacctggtgt cgctggtcgg cattcgccac gtggtgttgg ccgtcaacaa
     1561 gatggatctg gtcggattcg atgccggtgt gtttcatcgc attgccgacg attttcgtgc
     1621 ctttgccgcg ccactcggtt tcgaaagcat caccccgata ccgctcagtg cgctcaaggg
     1681 ggacaacatt accagccgtt ccgcgcacac gccgtggtat tccgggccga cgctgatggg
     1741 ttacctcgag tcggtgcgca tcgagccacc gcgcagtgac cgcttcgtat ttcccgtgca
     1801 gtgggtcaac cggcccgatt cttcgtttcg cgggtttgcc ggtaccgtgg cagagggagg
     1861 catcgccatc ggcgatgaaa tccgtgtgac cgcatcgggc cagaccgccc gcgtggctga
     1921 tctggtgacc atggatggct cgctggtctc ggtcgaagcg gggcaggcag tcacgctgac
     1981 gctcgaccac gaaatcgatg cctcgcgtgg cgacgtgcta tcgctggcga gcgcgccgct
     2041 cgaatccaca gaccagtttg aagcgatcgt ggtgtggatg aacgaggaca cagggttgac
     2101 cgggcgcaat tatgacatca agctgggcac gcagtgggcc tcggcgtcga tcaccaccat
     2161 caagcaccgt attgatgtca acacgctggc gcacgaggcc agccgcacgc tcgaactcaa
     2221 cgatatcagc gtatgcaatc tggcgctcag tcgtccactg gtattcgaca attatcgccg
     2281 ctcgcgcaca ctgggcagtt tcattctggt tgaccgcttc acgcacgcca ccgtggctgc
     2341 aggcatgatc cagcataacc tgcgccgggc gcagaacgta caccgtcagg cactgtccat
     2401 cacgcgcctg gaacgggaaa agctcaacgg ccatccaggg cgtgtgatct ggtttaccgg
     2461 gctgtccggt tccggcaagt ccacgctggc caatgcgctg gaggtgtcgc tgcatgccca
     2521 ggggtatcgc acctacattc tcgatggcga caatattcgc caggggctca acaaggatct
     2581 cggcttcacc gaagccgacc gcgtggagaa catccgccgc gtagccgaag tggcgcgatt
     2641 gatgatggat gccggcctga tcgtgatgac ggccttcatt tccccgttcc ggcgcgagcg
     2701 cgagatggcg cgtgatctga tcggcgcgga gcatttcctc gaagtttatg tcgacacgcc
     2761 gcttgaaatc tgcgagcaac gcgacgtgaa ggggttgtac aagaaggcgc ggactgggca
     2821 gattcccaac atgaccggca tcaacagtcc atacgaggca cccgagcagc cggccctgac
     2881 gctgcacagc agtgaaacgg cgattccggt gatggtgcag gcgctcgaag ggctgctgcg
     2941 gcatgaagcg cattgaattg cgtgcaggcg gaatgatcgg ggcgatatga cgagcttcaa
     3001 aaggcgggtg atcagtgccg gcatgtggac gatgggcggc tatgtcgttg cccaattcct
     3061 gcggctggcg agcaatctga tccttacccg tcttctcgtg ccatccatgt tcggcgtcat
     3121 gtccattgtg actatggtga tggtgggtat ggtgctgttc accgatctcg ggttgcgcga
     3181 gaacgtgatt cagagcccgc gtggcaatga tcctgctttt ttggatacgg tgtggacgat
     3241 ccagattctg cgcggaatcc tgttgtggtt tgtttccgtg ttgatcgggg gggcgttgta
     3301 ctttgccgac aggatgggct gggttcccgc gcacacggcc tatgccgatc cggtcttgcc
     3361 atgggtgata cctgttgcga ccttgagcct gattttttca gcgttcgagc caacctggac
     3421 ctcgaccgcc agtcgccggc ttgaacaatc gaaactgacg ttgatcgagc tggcgagcca
     3481 gctgggtggt attctggtca cgctggcgct ggttgcggta gagcgatcca tctgggctct
     3541 ggtggtcggc agcttgatac ccgccgtcat ccgcaacatc attgtgtatc gcatcatccc
     3601 gggtgcaccc aatcgctggc atctggaacc tgccgcagta catgaagtgc tacagtttgg
     3661 gaaatgggtg ttcctggcat cagtggtcgg ctttttgttc atgaatggcg atcgtttgat
     3721 gctgggagga atggtcagcg cccacgagct tggagtttac atggtggcct atttgctggt
     3781 gtattcggtg aaccaggcct tcggaaggct ggccagcagt gtcgtgtttc ccgcgatcag
     3841 cgaggtgcat cgcaatcgcc ccgagcattt cggtcggacg tattaccgtt ttcggttgtt
     3901 cttcgatttt ggcctgttgt tcatggcggg attcctgttc aatgccggtg aatggctggt
     3961 gcattttctc tatgacgagc ggtacgccgg cgcaggatat atgttggaga tcttgtcgct
     4021 ggagttggtg gctctgcgct acaatctggc ggatcaggga tttattgcca agggtgagac
     4081 caagcttgtt tccaacctga ttatcgtgcg taccttagta ttatttgtcg cattgccact
     4141 tggcgcgcgc agttttggaa tggtgggcgc aatgtgggcg attgtggcca gctattttgc
     4201 cagtgtgcca ttctcgttat attacaagta caaactgaaa tttctgcatc ctggcaaaga
     4261 gttatatgta ctgcccatgt ttttactggg tggtggattg ggctggctgt tgagccaggt
     4321 gacgctaagc tggcgccact gaccgcgagg tggagcagat catgactggc aagccacaat
     4381 caacgggcgc agcaaaaccg gccatttctg cgcagacacc cgactggtcg cgtgagcgca
     4441 aaccgcgttt tgcctggatg cccggacgcg cgctgctggc cagtctgcgt gcctatcagg
     4501 cacatgcggc ctcgcgcaat ccgctgcggc ggcttgtgct caagcgggtg gcggtgctgc
     4561 ggcaccgttt ctggagcgtg gtctgcggag cggacattcc gctcaactgt tgcatcggag
     4621 gcgggctgct gatcccgcat cccaatggca tcgtcattca tccgcaggca agcatcgggc
     4681 ccaactgcct gatcctgcag caggtgacca tcggtctgcg tgatggcgat gttccgccgg
     4741 tgatcggcgg gcatgtggac atcggagccg gcgcaaagat actcggcggg gtgtgcatcg
     4801 gcgatcacgc gcgcattggc gccaacgccg tggtgttgcg tgatgtgccg gcgggcaaga
     4861 ctgccgcagg tgtgccggcg cgcattctgg gggactgagc atgcgtatcg cctattttgt
     4921 caatcagtac ccgaaagtga gccacagttt catccgccgc gagatcctcg cgctggaggc
     4981 ggccggcaac gcggtgcaac gctttgcctt gcgcggctgg gacggtgagc tggcggatga
     5041 agaggatctg cgcgagcgcg aaaaaacccg ttacatcctg cagtatggtc tggtcgggct
     5101 gctgctgccg gtgctgcaac aggccgtatt gcatccgttg cgtctgctgg ctggtgtaca
     5161 gctggcctgg cgcatgtcga aacgtgctga tcggtctctg ccctatcacc tggtgtacct
     5221 ggctgaagcc tgtcggctgc tgcactggct gcaggctacc gcctcgcgcc acgtgcatgc
     5281 gcatttcggc tcgaattctg ccgaagtggt gatgctggcg cacgcgatgg ggggaccaac
     5341 ctacagtttc accgtacatg ggccagagga attcgacaag ccgcaagcca tcggtctgac
     5401 agaaaagatt cgccgggccg cattcgtggt cgccatcagt tcgttcggac gcagccagct
     5461 ctatcgttgg gtcgaccatt tccagtggcc aaaaatacat gtggtgcact gcgggcttga
     5521 agaagggttc tatcaggtgc cagcggtggc cttgcccgac aagccgcgcc tggtgtgcgt
     5581 gggccggttg tgcgagcaga agggccagtt gctgctgctc gaagctgcgc gccggttggc
     5641 actcgacggc gaggagtttg aacttgtgct ggccggtgat ggcgagatgc gcgcagaaat
     5701 cgaacaccgc attaccgaat atggcctgga caaccgggta cgcatcacag gctggatcag
     5761 cagcgcacag gtgcgtgacg agattctgcg cgcacgcggc atggtgctgc cgagtttcgc
     5821 cgaaggactg ccggtggtca tcatggaggc gcttgcgttg cgccggccag tgatcagcac
     5881 gtttgtcgcc ggcattccgg aactggtgct gccgggggag aacggctggc tggtgcctgc
     5941 cggcgatgtc gaagcgcttg ctgcggccat gcgcgaactg ttgcgggcgc cggtcgggca
     6001 gctgagcgcc atgggagagc tcggtcgcgc gcgcgcgttg gaacgccacg atatcgccac
     6061 cgaagccgcc acgctcgcgg cgctgttttc cagggcgggg gcatgattgc gcgggtgctc
     6121 gatgtctggg cagcgttgct gctggtgccg gtggccgtgc tgtgtgtcga gacattgctt
     6181 gcactgctgc cgacgcgacg ggcggttcct gttcgcgatg atgccacccg ggtggcggtg
     6241 ctcgtccctg cgcatgacga agccgaaggc attgcgccgg tcatcgatgg tatccggatg
     6301 caactgggcg cgcacgaccg gttgctggtg gtggctgaca attgcagcga tgccacggcg
     6361 accgtggcgc gcgagcacgg tgccgaagtg atcgaacgct ttcacgatac acagcgcggc
     6421 aagggttatg cgctggattt cggtgtacgc catctcgcgg cacagccgcc cgatgtattg
     6481 atcgtcatcg atgccgactg cacggtgcat gctggcgcca ttgaccggct ggcggcacgg
     6541 gtgagggtga cgaaccggcc agtgcagggg ctggatctga tgcgcgcgcc gccgcaggca
     6601 ggcatcaagg cgaaagtcgc cgagtttgcc tggttgctga agaatcaggt gcggccacgc
     6661 gggttggcgc gcatcggcct gccttgtccg ctgatgggca ccggcatggc attgccctgg
     6721 caggcgcttg cgcacgtgca actggccagc gcctcgctgg ccgaagacat gcagctgggc
     6781 atccatctgg cgcaacaggg gtttgcaccg ctgttcgagg aatcggcgct ggtgaccagc
     6841 gattttccgc tgacggcggc ggcccaggcc acacagcgca cccgctggga gcacggccat
     6901 ctgggcatga tcctcgcgca cgcaccgcgg ctgttgttgc agggcgtggt gcatgctgac
     6961 cgcaaccgga ttgtcatggc gctcgacatg agcgtgccgc cgctggcgtt gctggcgctg
     7021 ctgctggtga caggctggct ggcagggctg gctgccgccc ttgccggcgc gggcgtggtg
     7081 ccattgtgcc tgttcacgct ggcgcttgga atgtttgcca tggccatcct gctggcgtgg
     7141 tacggctggg ggcggcaggc gatcagcctg ggtgcgctgc tgcatgtgcc gctgtacgtg
     7201 ttgggcaaga ttccgcatta cctgcgtttt gtgttcaaac gggaaaaacg ctgggtgcgc
     7261 acgcaacggg acaaaccgga tggctgacga tttcgaccgt gatgtttact gcatggcagg
     7321 actgccattc gatgccgtga cgctgacccg caccctgggg atcctgtgcg acgccgcacg
     7381 ggcgcgtcag cgctgctttc tgtcgaccgc caacctgaat tatctggtaa ccgcgcaatg
     7441 cgatgcggcg ttccgcgatt cgatcctgtc cagcgacctg gtggtggtcg acggcatgcc
     7501 gctgatctgg atggcgcgct ggctgcagct gccgttgacg cagcgcgtgg ccggttcgac
     7561 gctgatcgaa gtcttgcgcg accagccgcg agacccgccg tgctcgattt acctgttcgg
     7621 tggccccgaa ggcgtggcgg aacgggcagc tgcgcagatc aatcgccatg cgcaggggtt
     7681 gcgctgcgtc ggcttcgatt cgccgggatt tggcaatgtc gagtcgatga gcgccgctgg
     7741 gcagattgag gccatcaatg ccagcgaggc cgattttctt gtggtggctt tgggtgcggg
     7801 caaggggcag ggctggatca tgcacaatct gcatcgtctg agcgtgcctg tcgtcagcca
     7861 cctgggggca gtggtcaact tcgaggccaa taccatccgg cgcgcaccgc gctggatgca
     7921 acgttccggg ctggagtggg tgtggcgcat ccgtgaggag ccggcgttgt ggaaacgcta
     7981 ctggcgtgac ggcctgcagt ttctgcgcct gctgctgcgc gatctgttgc cactcgggtt
     8041 ggtgatgcgc ctgcgcgaca aggcgccggc tcggggtgaa ttgcagttgg tgaacgaaga
     8101 cgacggtgtg tgccggttgg cggtgatcgg tgtgatcgac gatccggttg gcgagacgac
     8161 gcggtccacg ctgcgcgcat gcgcgctgcg cggacgcgac gtgacgctcg atctggcagc
     8221 agcgcagcat gtcggtgcgg ggttgctcgg cttgctgttg gtgctggaga aacatgtgcg
     8281 acgcaacgcg cggcagtggc gcgtcactgg tgcgacccgc aagatgcggc gctatctgcg
     8341 ttgtagcgga ttgcagcgct gggtggagta agcacctgaa cagacgttgt cggaggaatg
     8401 gggatggagc acgtggtatt gagcggccgg ggcatccagc gtttcgccgc gcagagttgt
     8461 atcaatgaat tcgaagatgt gatcgtgcgc acgctggggg cacaactggt gtgcccggag
     8521 agcggggaca acatggctgc gcttgatggc ctggctgggc aggataccgt ggcctggctg
     8581 gtgtgcctga ccatccacca ggttccggcg ctgctggctg cagtgccacg ctggcgtaag
     8641 cggtttgctc gtgttgtggt ttatgtgttt gattcgtacg agcggtttcc tgagtcagtc
     8701 acgttcagac agcgctattc acgcagtatg cgcaccatca atcaggtgga ccggatctac
     8761 atcgcgctcg gtg
//
LOCUS       DAIANL010000141         6475 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_792138, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000141 DAIANL010000000
VERSION     DAIANL010000141.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792138"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1363 a         2076 c         1783 g         1253 t
ORIGIN      
        1 ccggtcgcca cggtaaccgg cgccgacgcg tgcggcttcg agcatcgcgg cgccgccgag
       61 cgcatgcaca ttgagcatcc acacgcccag atcggccgct gcccggcaag ccagttcaac
      121 ggtggtcgga atgtcgtgaa acttgaggtc gagaaatacg tcatatccca gcgccatgag
      181 ccggctgacg cactgtggcc cggcggcggt gaacagttgc ttgccgacct tgagccggca
      241 cagttcgggt gacagttgcc gggcaagtgc aacagcgctg tcgcagtcgg cagtgtccag
      301 cgcgatgatc aggggagaat tggcagtcat tcttcttttc tttggggtgg gaaactgtcc
      361 cattgcccgc aagccgggca atgccagaaa aagttgcgcg cacgaaaacc gcagtgcgtg
      421 cactgataca ccatctgcgc ctgagcgtag cgctgtacag cgctacgtga caactgcaga
      481 tccggctggc gttcggctgg cgccagcagc atttcagcct cgagcagccg gtccagcgtg
      541 cgcaggctgg cactgcgctg caattcttcg cgcgccagtt cacaggccac ctgcgcccct
      601 tgcagccgca acgtggcgga aaaaaccgtg ctgaacagtt catacgaggg atgacgcgac
      661 aatgcgctgc gcaggaacac caacccttcg gcctcacgtc caagttcgcg gtaggcggtc
      721 atgatccggt ctgcgacaag tgtgagatgc ggaatgctct gtgcctcgat gcggcgccag
      781 gctgtcaatg cggcggagtg ttcctcgcga ctggctgcga tatcgccaag cagcatgttg
      841 gcacgcacgc attcacgatt ggctgtcagc gcctcgctca ggtattctcg tgcgacatcg
      901 acctgttcat tgagccagga ctggttggcg agttcgcaca ggtaatgacc gatctcgacc
      961 tggtaagact ggttcgacac gcgcgacaga ttgcgcgcgg cggagaccgc gtgattccag
     1021 tctttttcct gtacatagat ttccagcagg aacgcctgcg cctgtccggc catctcacct
     1081 tgctgcaacc gggtaaaaat cgcctcggca cgatcaagca gtccggcacg cagataatcc
     1141 tgccccaatt caaatcgcgc acgcaattgt tgctccgggt tcaggtcctc gcgctcgacc
     1201 agattctggt gcatgcgaat cgcgcgctcg acctcaccgc gcttgcgaaa cagtccgccc
     1261 agggcaaagt gcaggtcgac cgtatcgctg tcgactttga ccacctcgat gaacgcctcg
     1321 atcgcccggt cgggctgttc gttgagcaga taattgaggc cacggaaata ggaagccggc
     1381 acactgcgcg attcggtcat cacatgacgg atgtccacgc gcgcggccag ccagcccaga
     1441 ccaaaaaaca acggcagggc cagcagccac cacagttcga attccatgat ttcagatccg
     1501 tccgaccatc aagtcttgtc ctgtgtttcc ggcaatgtgc gacatgcggc cagcgcctct
     1561 cgcaagcgct gcagttccct gcgctggcgg tacagatacc cgagacaggc catgacgcca
     1621 aaagccgcgc ccagggtaaa caccacgagc acgaccagca ccagcggtgc ctgccactgc
     1681 cacccgagat agtaacgcaa taccaccggg tcactgtttt tgacggcaaa actcagcaac
     1741 aaggcaaaca gtgccagttt cgccagaccg ctgacataac gcattttgcc gttcccatgc
     1801 ccgaacaagc cggaagtgtg cgggtttcac gataccctgt caatgttgca ggctttcatg
     1861 cttttcccgt tacctgccga tgttgcaggt cggcaactct tttggtgttg cacatgccag
     1921 cttcgtcgcc aatgcggcgt gctcaaaaaa aaacggcgca ccttacggtg cgccgctgtg
     1981 ttacgggctg catgacccat cagctggacg gatagtcgac ccgctcgcgc aattccttgc
     2041 cggccttgaa gtgcggtacg tatttttccg gcacctcgac cttttcaccg gtttttggat
     2101 tgcgtccaat acgcggcgga cgataattca ggctgaaact gccgaatccg cgaatttcca
     2161 cccgcttgcc gtcggcaaga ctgcgcgaaa cagcgtcaag aatggttctc accgccattt
     2221 cagcatccgt tgccaccagt tgcgggtgac gaaccgccag ttgcgcaatc agttccgatt
     2281 tggtcatgtc gccacctttc aggattcaac gttgcggtgg tcgagcttgg ccttgagcaa
     2341 cgcgcccaga ttggtagtac cggtattggc accctgatcg tccgccagct tctgcatggc
     2401 ctcattgtgc tcggcggcgt ccttggcctt gatcgacagg tggatgctgc gattcttgcg
     2461 gtccacgttg atgatcatgg cttccacctc gtcaccttcc ttgaggtgat tacggatgtc
     2521 ttcgacgcgg tcacgcgaga actcggaagc acgcagatac ccttcgacat cgttgtcgag
     2581 ctggactacc gcacccttgg catcaagcga tttgaccttg ccgcgcacga tgctgccctt
     2641 gtcgaaactg gacacgaaac tgttgaccgg atcactttcg atctgcttga tgccaagcga
     2701 gatgcgttca cgttccacgt cgatggccag caccactgcc tggacttcgt cgcccttgcg
     2761 gaaattacgc acggcttctt cacctggttg cgtccacgac agatcggaca gatgcaccag
     2821 accgtcgatg ccacccggca agccgatgaa cacgccaaag tcggtaatcg acttgatctg
     2881 gccggtgaca cgatcgccct tcttgtgagt ggcttcgaag tcatcccacg ggttggattt
     2941 gcactgtttc atgccaagcg agatgcggcg acgatcttcg tcgatttcca gcaccatgac
     3001 ttcgacttcg tcaccgagct gaaccacctt ggacggatgt acgttcttgt tggtccagtc
     3061 catttcggag acgtgcacca gaccttcgat accggtctcg atttcgacga acgcaccgta
     3121 gtcggtcagg ttggccacct tgccaaacat gcgtgtacct gccggatagc gacgcgacag
     3181 accattccat ggatcgtcgc cgagctgttt gatgccgagc gaaacgcggt tcttttcctg
     3241 atcgaacttg agaatcttgg cctcgacctc atcacccacc tgcaccactt ctgaaggatg
     3301 cttgacgcga cgccaggcca tgtcggtgat gtgcagcagg ccgtcgatgc cacccaggtc
     3361 cacgaacgca ccgtagtcgg tgatgttctt gacgacgccc ttgacgatgg cgccttcctt
     3421 caggttggcc agcagggctt cgcgatcggc gcccatggtg gcttcgagca cggcacggcg
     3481 cgacacgaca acgttgttgc gcttgcggtc gagcttgatg accttgaact ccatctcctt
     3541 gttttcatag ggagcggtgt ccttgaccgg gcggatatcg accagagatc caggcaggaa
     3601 ggcgcggatg ccattgatca tgacggtcag gccacccttg accttgccgt tgaccatacc
     3661 ggtgatgatt tcgccatctt ccatggattt ttccagcccg atccaggctg caagacgctt
     3721 ggccttgtcg cgcgacagac gggtggaacc atagccatct tcgagcattt cgatggcgac
     3781 actgacgaaa tcgccgacct tgacttcaag ctcgccacgg tcggaacgga attcttcaac
     3841 aggaatgaga ctttcggact tgagtccggc gttgaccgtg acaaagttgt catcgatcgc
     3901 aaccacttcg gcggtaatca cttcgcctgc gcgcaattcc tgacggctca ggctttcttc
     3961 gaagagggcg gcaaaacttt ccatgccgta atcggcctgg gatgaggtaa cagtagacat
     4021 gaacaataac ctgaataacc cgcatggctg cggggtaagt tacagacggg cgtgcagcag
     4081 gcacgccgcc caacacgccc ggcaccgtca aaacagtccg ggcacccttt cgcgccactg
     4141 tgccagcaca aaggcaaccg ctgcggcgat gcccatggcg ctggtatcca gcagcaccga
     4201 atctgcctgc tgcaccaacg gcgcgacggg acgattggca tcccgtaaat cgcgttcacg
     4261 caagtctgca agcagaatgt cgattgtagc aggcattccc ttttcgatca actgcttata
     4321 ccgccgttcg gcgcgcactt cggcagtggc cgtcaaaaac accttgagtg cggcatcggg
     4381 aaacaccacc gagcccatat cgcggccgtc ggccaccagg cccggcagtc gccgcatggc
     4441 gcgctgacgc gccagcagtg cctcgcgcac cgccggaaaa cgggccacac gggatgcgcc
     4501 tgcactgcag gcttcgccgc gcaattcttc atcgaccgac tctccagcca gccgcgcttc
     4561 gccgccatcg aacgacacat cgagaccagc cgcaagccgc gccaacccgg cttcatcatc
     4621 gaacgaaacc tgagcccgct gcgcggcaag tgccaccaac cggtaaagcg aaccgctgtc
     4681 gagataatga aaccccagtt cgcgagccac ctgcgccgcc accgtgccct tgcccgaggc
     4741 cgatggcccg tcgattgcaa tgacaggcgg catcatgccg gctccggacg tacaatggct
     4801 tcaaatgcct cgaaataatg tggaaacgtc ttgttgacgc attgcgggtc attgatccgg
     4861 atcggcacgc ccgcaagcgc caccagcgaa aagcacatgg ccatgcggtg gtcgtcatag
     4921 gtgtcgatcg tggccggcag aaagcagtgc ggcggcacaa tgcgcagaaa atccggcccg
     4981 gcctcgactt cagcccccac cttgcgcagt tccgtcgcca tggctgcgat ccggtcggtt
     5041 tccttgacac gccagctgcc gatattgcgc aaccggctct cgccgcgggc aaacagcgcc
     5101 aggatcgcca ccgtcattgc cgcatcgggg atatggttga aatcggcatc cactccacgc
     5161 aattcttcgc cgcaactggc agtgatcgcg tcgtcctcgt aatcgatgat cgctcccatc
     5221 tgcgccagca cttcggcaaa gcgcacgtca ccctggatgc tgttccgccc caccccttcc
     5281 acacgcaccg ggccgcctcc aatcgcacca gccgccagaa aatacgatgc cgacgaagca
     5341 tcgccctcga cttcgatccg cccgggggag tgatatttcg ccccggcaga caccaggaat
     5401 tcgtccggcc cgcgactgtc gaccacgacg ccgaaacggc gcatcaggtt gagcgtgata
     5461 tcgacatacg gccgggaaat cagttcaccg tccaccacca gggaaatatc gcgcccggac
     5521 agtggtgccg caagcagcaa cccggtaaga aattgactcg acacattgcc gcgcaccggc
     5581 acacgggctg gcgcagcttc cgcggacggc gaaatctgca acggtggata accttcagcc
     5641 cccagataat cgatatgcgc accggccagc cgcagcgcat ccaccaggtc gcgaatgggc
     5701 cgttcgtgca tccgggcatt gccggacagg cggtaatgcc caccggcaaa cgccagcact
     5761 gccgtcaggg aacgaaacgc cgtgccagcg tttccgagat aaagcccggc ctcgcgccgg
     5821 ggaaacacgc cagcgcaccc ctgcacccgc acttcgttgt cgccgacgcg ctcccagcct
     5881 acgcccagct gagccaaccc atcgagcatc acgcgcgtgt catcggaatc gagcagccgc
     5941 acgatgcgtg tctcgccctc gctcagcgct gcaagcaaca gaatccggtt agacaggctc
     6001 ttcgaacccg gcaggcttac cgtgccttgc gcggcggcca gcgggggcaa atcaagaaac
     6061 ggcatcatct tctgcactat cctgcaagaa ttcgcaccat gcgggacatc cagccgcagc
     6121 gtgcgacaaa taatgaaata caggcgcgga ttgtagcgca tcttgcttgc aatacaatgt
     6181 cgatcagacc aaaagcgtcc ctgattgacg cataatcatg accaccggcg ttgccgtccg
     6241 ccggtgacaa acgcggattg gacgtcacaa tgcaccgccc cgcccgcacc gcccgaaaac
     6301 aaccttctgg aacaaaccga tgaaacattt tcgcaaggaa ctctggttca acattcccac
     6361 ccgccgcgct ttcgtcaaca tcaccagcca ggtggcagat tgtgtaagac aaagtggcat
     6421 acgcgaagga ctcgtgctga tcaatgccat gcacatctcc gcatcggtgt tcatc
//
LOCUS       DAIANL010000142         7931 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_592217, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000142 DAIANL010000000
VERSION     DAIANL010000142.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7931)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592217"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1432 a         2152 c         2769 g         1578 t
ORIGIN      
        1 cgagcctgcg gccggcgaat tcggattcct ggacgtatac cgtaccgact tcggcacgct
       61 ctgcatcgct gagcacgggg taattatgtg cgcccggaat atggtcgagc gcaaattcgg
      121 ccggggtgcg cacatcgatc agctcgtcaa agccggtcag atgtgctagg gttatgcggt
      181 tattcacgtt ggattggcag gcatggaatc gatcaggttg acgcaatttt cgcacggcgg
      241 cggttgcggt tgcaaaattg cgccttcgct gttgcgggaa ctgctatccg gtatgccggg
      301 cggactggcg tcgggtcagt tgctggtcgg cagcgaaacc agtgacgatg cggcagtgta
      361 tcgcctgaat gaccggcagg ccattgttgc cacgacggat tttttcatgc cgatcgttga
      421 cgatccgttc gatttcggtc gcatcgccgc aaccaatgcc atttcggacg tgtatgccat
      481 gggtgggcgg ccactgtttg cgctcgccat tgtgggcatg cccgttggca agttgccggt
      541 cgaagtgatt cgcgccatcc ttgccggcgg cgagtccgtg tgtcgggagg cgggcattcc
      601 ggtggccgga gggcattcga tcgattcggt ggaaccgatc tacggcctcg tggccatcgg
      661 gatcgtcgat ccgcacaaga tcaaacgcaa ccgggatgcg caggctggcg acgtgctgat
      721 tctcggcaag gcgcttgggg tgggcgtgct cagttcggcg ctgaaaaaag gacggctcgg
      781 tgatgtcggt tatgcacaga tgctgtcggt gacgacgcag ctcaatacgc cgggcatggc
      841 gctcgccgag cacccgggag tgcatgcgct taccgatgtc accggtttcg gtctgctggg
      901 ccatctgctg gaaatctgcc gcggttcggc gctaagcgcc gagatcgagt ttgcggcgtt
      961 gccggtgctg tcggaagcgc gcgaactggc tcgtcagggg catgcgccgg gtgcggcggc
     1021 gcgcaactgg agcggatacg gttcggacgt gcgcctgccg gacggcatgg agtcgtggca
     1081 gcgcaatctg ctgtgcgatc cacagaccag tggcggtctg ctggtgtcgt gcagcgagca
     1141 ggcagcccag gaagtacttt caatgtttgt cagggccgga tttcccgatg cctgtgtgat
     1201 cggacggctg gaacgcggct tgcctgcgat acacgtgagc tgattcgatc ttgggcgtgc
     1261 gggcgcatgc ccgcctgcgg gtcgcagccg tccggttgcg acacctaaag tttaaccttt
     1321 caaactcatc ccatctatct gatttatcat ttaatgactt tgcacgtgca tgcgtggcag
     1381 gtcggctaag tcattgtcag tatttgaaaa tcccttgtgc ttgcttgacc cgtggcattt
     1441 tcttctctat agtgggtgca ggtggtaatt tgtggtaaaa agtgggtaaa attccgcggc
     1501 gcgtccggcg aggaagagca tgtttcgtgg cgtaaccaca ttgagtctcg acagcaaagg
     1561 gcggctgatg gtgccggccc ggtatcgtga cgagttggtc ggccaggggg acggtcgggt
     1621 cgtggtgacg gccgatccgg ggaagtgcct gctcctgttc ccactggtgg aatgggagcc
     1681 catcgagaaa aaactcaaca gcctgtccag tttcaacgcg cagacccgca gtctgcagcg
     1741 cctgttggtc ggcaatgccg ccgatattga gctggacagt tcgggtcgca tcctgttgcc
     1801 ggccatgctg cgcgaatttg ccgcactgga taaatcggtg gtgctggtgg ggcaaggcgt
     1861 gaaattcgag ttgtggaatg aagtgcgctg gcaggaacag atggaccttg ccctgacgtt
     1921 tcgtgatgcg gaaatgccgg ccgaactggc cggcttttcg ctgtgaacac ggagtctggt
     1981 catgaaacag tcctgcgcga ggaagcagtg caggcgctgg ctgttcgccc cgatggcgtt
     2041 tacgttgatc tgacgttcgg acgtggtggg cacagtcagg aaattttgcg ccggctgggc
     2101 gatgcggggc gagtgatcgc gctcgatcgc gatcagcagg cggtggcagc gggagcaggc
     2161 tggcgggatg cgcggttcac cattcgccac gcgccgtttt cgcagatgga tgcagtgctg
     2221 gatgaaatgg gtgtccggca ggtgaatggg atagtgatgg acctcggtgt gtcttcgccg
     2281 caactggatg aggcggaacg gggattcagt ttccggcagg atgcgccgct cgacatgcgc
     2341 atggatcaga catcgggcct tacggctgag caatggttgg ccggggtgga agaaaccgaa
     2401 ttgagggagg tgctctggca atatggagaa gagcggcatg ctaaagcgat tgcaagagcg
     2461 attgtcgcgc aaagagcgcg tgcgccgatt gcaaccactc accagcttgc gcggctggtc
     2521 tcgtccgtcg tgccaggtcg tgagacgggg catcacccgg ctacccgaac ctttcaggct
     2581 gtacggattt acatcaaccg cgaacttgaa gaactgtcgc tgaccctgcc acgggcggcc
     2641 gaccggttgg cgccgggcgg ccgtctggtg gtgatcagct ttcactcgct tgaagaccgc
     2701 atggtgaaac gtttcatgcg tgagcgttcg agtccgccgg ctctgccggc cagattgccg
     2761 ttgcgcgcgc atgaaatacc agccgcgcgc atgcgtctgg cagggcgcgc catgcgcgcc
     2821 ggcgaacgcg aagtgctgaa caatccacgc gcgcgcagcg cggtgatgcg tgtactggag
     2881 gcgttgtgat gcgcttgcaa ctggttttgc tgtccggagt ggtggtgtgc gccctgtcgg
     2941 tcatcgatgg ccagcaacag gcacgcaagg ccttcatcgc cctgcagcag gcacagcagc
     3001 aacagcggca gctggaagaa gactggggcc ggctgcaact ggagcagagt acctgggcca
     3061 tgcatgcgcg cgtaggcaag atcgccaccg agcagttgca gatgcaactg ccggatgcat
     3121 cgcagatccg tacgctgttc gctcctgctg tacaaggctc ccggccatga tggcgcgcat
     3181 gggcaagccg tcgggccatt tgctggcgct gcatctcaag cagtggcgca gtacattcat
     3241 tcttgcactg ttgttggtct ggctgtcggc gatggtcggt cgcgcggcgt atctgcaagg
     3301 cttgcgccat gctttcctgc aacacaaggg ggatgctctc gccaaccgtg tggtggagat
     3361 gccggcgcat cggggcatga tatttgaccg taatcgagag ccgcttgcta tcagtactcc
     3421 ggttgaatcg ctgtggtgca acccggccga cgtggaagcg agtccgggtc aggtacggca
     3481 gatcgcggcc attttgcaga tgaaaccggc cgaagtgcgg gacaagctcg aggacagcag
     3541 gaatcgcggg tttgtctata tcaagcgtcg tctgccgccc gaccaggcag aagcggtgac
     3601 ccggctcgac attccggggc tgtctttgca gcgcgaatac cgtcgctatt atcctgccgg
     3661 cgaagcggtg gcgggactgc tgggtttcac cggtgtcgat gacagcgggg aagaagggct
     3721 tgaactggcc tggcagaatt ggctggccgg caagcctggc agtcagcgcg tgcttgaaga
     3781 ccggcacggt aacgtcatcg aggacgtgca aagcatcctc agtccgcgtc cgggccatga
     3841 cttgcaactg gcgatcgaca tgcggctgca atacctcgcc tggcatgaac tcgaactggc
     3901 ggtgactgcc aacaaagcgc gttccgggtc gattgtggtg ctggatgcgc gtaccggcga
     3961 agtgctggcg atgaccaatg tgccgtcgtt caatcccaat aatctggatg gggtcaagcc
     4021 ctggcagatg cgcaatcgtg cggttaccga tcagtacgag ccaggctcga ccctcaagcc
     4081 tttcaccgtg accgcggcgc tcgatacggg ccgttacagt ccggaaacag tgattgatac
     4141 cgaaggcggc agctggcagg tcggcgtgaa ccggattcac gatgcgcatc cggaaggcat
     4201 gcttaccgtg agtcaggtga tcgaaaagtc aagcaatgtg ggtgcatcaa aaatcgcttt
     4261 gtcgctcgaa tcgcaataca tgtggcaaaa actgcacgat tgcggattcg gagtcgcgcc
     4321 ggtgagcggt ttccccggtg tggccgaggg tgacctgcgt ccatggcagc actggcgtcc
     4381 gatcgagcag gcaacgatgt cctatggcaa cggaatctcc gtcagcctgc tgcagttggc
     4441 gcgagcctat accgtattca ccaaccatgg tgtgttgctg ccggtcagcc tcgtggaagg
     4501 cgccagaaac acctctcagc ccgtgcgcgt gtactcggaa aaaagcgctg acgaaatggc
     4561 agccatgctg gaaaacgtgg tgacgccgga gggcacggca ccgctggcga gtctggccga
     4621 ttatcgcgtg gcaggaaaga caggtactgc gcacaagccg gatcacggcg gctacagcgc
     4681 caatcgctat atcgcatcgt tcatcggatt tgccccggcc agcagaccgc ggctgattgt
     4741 ggcagtgatg atcgatgagc cctcggccgg tcagtattat ggcggaacag tggccggacc
     4801 ggtattccat aatgtcatgg ataacgcgct gcacctgctt gatgtggcgc cggacaaggt
     4861 gatgccggtg catgcagggc cggacgaaac cggtaaggcg gcaagaacat gagcccggtc
     4921 gtgtggcagc agccccagct gatcctggct gaattggccc ggcgcggtgt cgtggcgcat
     4981 gggctggtga ccgatagccg taaagtccgg gcggggtggg tgttcgctgc gtatcccgga
     5041 gagcggctcg atggacggcg gttcattgcg caggccgtgg ctgcgggagc tgcggcagtg
     5101 ctctgcgagg tcgctgacgg atggaacgag ccgttgccat cagcgatcgt gatcctgccg
     5161 gtggccggac tcaaattcat gatcagcgaa ctggcggatg cgatgtttgg tcatccgtca
     5221 caggcgctgc gggtcattgg cgtgaccgga accaacggca agacgtcttg cgctcactgg
     5281 ctggcgcaga cactggctgc cgctggcaca ccggctgccg tgatgggcac actgggcaat
     5341 ggtgtgctgc ccgatctcga acctgcggac aataccactg gcgatgcagc gttgctgcaa
     5401 cagcaactgg cacagtatcg ggctcagggc gtgcgtgcgg tggtgatgga ggtgtcatcc
     5461 atcggtcttg agcaacgtcg ggtagaggcg gtgcgtttcg atacggcgtt gctgaccaac
     5521 ctgagccggg atcatctcga tgtgcatggc gacatggatc ggtatgcggc ggccaaggca
     5581 cggctgttca gctggcccgg attgcgttgc gcagtcatca atgcggacga tgctttcgga
     5641 gcggcgctgc tgcgcgaggg gggggcacag gctgacgcag tgatgggtta cggtttcggc
     5701 acggccgagg tgcgcggccg gtcattgacg ttgtcggctg ccgggttgga catgcaggtc
     5761 gatactccat ggggcggggt gcgcatcgtc agccacatgc tcgggcactt caatgcctcc
     5821 aatctgctgg gggtgctggg ggtgttgctg gcgggcggca tggaccgcga acaggctgcc
     5881 ggattgctgc agcaactgaa gccaccggca gggcgcatgc agacgctggg cggaggcgcg
     5941 cgaccgctgg tggttgtgga ctatgcgcat accccggatg ccttggaaaa ggttctgacc
     6001 agtctgcggg agctggctcc ggctgcgcgg ctgttgtgcg tgttcggctg cggcggcgaa
     6061 cgcgatgccg gcaagcggcc gctgatgggc gccatcgttt cccggctggc ggacgtggcc
     6121 gtggtgactt ccgacaatcc gcgtggcgag gccccggagc gcattcttgc ggatattcgc
     6181 gcgggcatgg acggtcgcga gcaggtggaa accgatcgtg gccgcgccat cgagatcgcg
     6241 ctggagcagt cccggccggg agatattgtg ctggtggccg gaaagggaca tgaggcctat
     6301 caggaaatcg ccggcgtaag acaccccttt agcgatgctg cagtggtgat gcactggctg
     6361 gaggcgcgca catgggcatg atgactactc gcgaagcagc gctatggtgc gatggcgaga
     6421 tgcgcggtgc ggaaacgccg attctgcgcg tgacgaccga cagtcggcag atccagccag
     6481 gtgacctgtt cgttgcgctg cgcggcgaac atttcgacgg tctgcaattt gctccggcgg
     6541 cgctggctgc gggcgcggca gccgtcatgg tgagcggtga ggccaaggca aagctggagc
     6601 cggcggtggt ggtggccgat acccgcattg cgcttggacg gcttgccagc ggctggcgca
     6661 tgcgcatgcc ggctaccgtg gtggcaatca ctggcagcag cggaaaaaca acggtcaagg
     6721 aaatgctggc tgcgctgttg cgtgcgcaga ccggcgatga cgccgtgctg gcaaccgtcg
     6781 gtaacctcaa taacgacatt ggcctgccac ttaccctgct cggcgtacgt cagcagcatg
     6841 cctttgctgt gctggaagcg ggaatgaatc atgcgggcga actggcctgg ctcagcgaga
     6901 tcgcgcaacc cgatctggtg ttggtcaaca atgccggcag tgcgcacatc ggcctgctgg
     6961 gttcggccga ggcggtggca cgtgccaagg gggagctcgt ggaggccagt ccggcgcgcg
     7021 ccatcgtcgt gctcaatgcc gacgaccgtt tttatccact ctggtgcggg atggccggca
     7081 cgcgccgcat cgtcagcttc ggcatgaatg cgcaggcaac ggttgccggg agttgggtgg
     7141 cgactggcgc gggcggcaaa ctgcaggtgc ggctggatca ggacacgttt gaattgccgc
     7201 tgccgctggc cggtcgccac aacgccatga acgcgctggc ggcaattgct gctgcatacg
     7261 cgctcggcgt ggagagcacg gcaatgcgcg ccgggctggc ccgagtacac ctgcccaagg
     7321 gccggctgca actggggcat gcggcgcatg gttcgcggct gattgacgac agttacaacg
     7381 ccaaccccga atcggtgcgt gcagcaatcg atgtgctgtg cggattttcg gggacacgga
     7441 tactggtgct gggcgatatg ggcgaactgg gcacctacag cgacgaagcg caccacgcca
     7501 ttggtcagta tgcgcgcgaa gcgggcatcg aggcgttgta cgcgctcggt acagccagtc
     7561 gtgcgaccgt ggccgcgttt ggcgacggag gagaacattt ttccgatgcg cacgcattgg
     7621 cgcaggcagt gcaggcgcgt ctggatgcgc agaccaccgt gctggtcaaa gggtcgcgtt
     7681 tcatgggcat ggagcgtgtg gtgaatttgc tggaggaaaa cgactgatgt tgctgtggct
     7741 gtttcagtcc ctggagccgg aagtgcatgg gttcaacgtg ttcagctaca tcacgttgcg
     7801 cgccgtgctg gcatcgctga cctcgcttgc catttcgttt ctggtggggc cggcggtgat
     7861 tcgcaaactg actgcgctga agatcggtca ggcggtgcgt gacgatggtc cgcagacgca
     7921 tctggtcaag g
//
LOCUS       DAIANL010000143         8980 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_184942, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000143 DAIANL010000000
VERSION     DAIANL010000143.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184942"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1741 a         2530 c         2914 g         1795 t
ORIGIN      
        1 ccgtcccgca accgcgccag cgtatcaatc ggcgcaggct ggatggcccg cagcacaaca
       61 ctttaccagt cgcatacctc cgattgctcg atgagctgtc ggggcgtcat gtcacgtccc
      121 gtgcgcagtc accgtggatc agggcgcgat cggttgcagc acatccgccg gcgtgccccc
      181 gcgtggatca gggcagggtg ccgcagcttc cagcggagct ggtgtccggg cagccaggta
      241 cgttggaagg gatctgtccg agcatgcttt tcagggagat gttgtcgcgg ttaaactggc
      301 ggtcatagaa gacggcattg gcaaatactt tcttcacgta gtcgcgtgtt tcgctgaaag
      361 gtatggtttc agcataaatc gcgccttcga cggcctgtgg cgggcgccaa cgcagtgcac
      421 gattaggacc ggcattgtag gcggccgtgg ccagcaaatc tgaatcgcca acacgttgca
      481 aaacctgctt gaggtaatac atgccgaaat gcaggctgat gtcgggctgt tcgagccgcg
      541 atacgcggaa atgacgcacg ccaagacggt gggcgatcca gtgcgcggtc gacggcatga
      601 tctgcatcag cccgcttgcg cctgctccgg agcgggcgat gctgacaaag cggctttcct
      661 gacggatcag accgtaaacc caggcttcat cgagcccgta ctgattggca tagatgccgg
      721 caacatcgcg gtaaggagta aggaagcgca ggttgaaatt atgtagctgg cgcgtccggt
      781 cagcggtatt gatggcacaa tccagccaac ggtggcggcg cgccagctcg gcggcggcga
      841 gcagttgccg gtcgctgaaa ttgcggatgg caaagcgcca ttcgcgcaag gcttccagcc
      901 gcaggccgaa ttcatgcagc atcagagccc ggcgaatacc gggcaattgg cccacagtgt
      961 ccagttcctc accggaaaca gcgtagttga tggcgggcgc actggcgacg gcacccagtt
     1021 cttcctgtgc gagcaggccg tagaagtcga attgatgaga cagcgacagc cagatcaggt
     1081 tggcggcctg ggtgtggccc agattgcttt cactccgggc cagccagtag cgccattcgg
     1141 ggagcgcacg gccggcatcg ctcatcgcca gaatgctttt gtgcaccgtg gtccagtcgc
     1201 cggcaagcag cgctgcgcgc actttccagc ccagctgatc atcgtcaagc ggctgcgatc
     1261 cggccagtgc aaaccattgc tgcgattgcg gcaggtgctg gcgagcagcc tgggtcgcga
     1321 tcacggccca cagtttcgcg cgcgtttgcg ggtcgaaatg ctgttgccac gtctgccaac
     1381 tggcagcgct ggactgggca tccttacgcg atagccgttc gatggcaaag cgaaccagct
     1441 cctgatcggc gcgctgttgc aggtcgtgag tgccggccag gaatgcaaca ggatcggcct
     1501 gcgcctgttc cagccgggaa aggctgacgg gtaacggcgg cggtagccag tcaaacgacg
     1561 tttgtgccga cgcgatgtcc atggtggcca gtgcgtggcg gaaatgtagc catgcatcct
     1621 gctgggtgag ctggtggctc gcgaacatct gaccgaacaa cgtgttgcaa ctggccggaa
     1681 ccggcctggc cgagcgccag agtgcaacag cctgcggttc ggcttctgcc ggtttcatgg
     1741 cgagttgccc ctgccacgca tcgcattgca gctcgatgtc cgggttttcc tgttttgcat
     1801 agcgcacgag aaagtcgggc caattctgct gtcgtgccag ctggcgcaac cagtcgactt
     1861 gcagtctcgc tgcaagcgga gagtcatcgt ggttggcgac gaatgcgtcg atgtctgcat
     1921 tgctggcctg ctgaaggttc aggttgatgc gccagtaatc gatgtagggc cgcaggatcg
     1981 agtggtgcag tcgagcggca aaatgctcga aacgaacgga atcgccgacc cggtaggcgt
     2041 catgcgccgc gactacgtcg ctaccttcgt cggccatgac tgcgtacggc aagaacagta
     2101 acaggcagcc cagcaacagc cgcgcgcaga ttggcctcat ggctagccca gaaacagccg
     2161 ataggcaatg ttgtcggatt catcccacca gggatagccc agacgctcga gcgattgcag
     2221 aaactcggca cgttccgtaa gcggcacctg aattcccacc agcacacgtc catagtcggc
     2281 gccatggttg cgataatgga acaggctgat gttccagtgt tcgctcattg cattgagaaa
     2341 ctgcatcagg gcaccgggtc gttcgggaaa ttcgaagcgg agcatcagct cgtcatgcac
     2401 ctgtggcgca tgccctccaa cgaggtagcg cacatgcagt ttggccatct cgttatcggt
     2461 caggtcaacg gcagtcaggc cgtttccgtg cagcgacagc accagctcgg caaccgcctg
     2521 ccggttcggt acctggatac cgacgaaaac gtgggctgcc tgactgtcgg cataacggta
     2581 gttgaattcg gtaatgttgc gcgtgcccag ttgggcgcag aatgtcttga aactgccggg
     2641 aacttcgggg atggtgacag cgagaacagc ttcacggcgt tcgccaagtt cggcgcgctc
     2701 ggcgatatga cgcagccgat cgaagttcat gttggccccg ctggcgactg caaccacggt
     2761 cgcgttgctg acctgttccc gcgcgcacca caccttggcg ccggccacgg acagcgcgcc
     2821 tgaaggttcg aggatcgacc gggtgtcttc gaatacgtcc ttgatggcgg cacagatggc
     2881 atcgttgtca acgcggatga tttcgtcaac atagcgttgt gccagacgga aagtctcttc
     2941 gccgacacgc ttgacggcca ccccgtcagc gaagatgccg acatgggtga gcaggatgcg
     3001 ctcaccactg gcgagtgatt gtgccatggc atctgcatct tcgggttcga cgccgatgat
     3061 gcggataccg ggcttgaggt gcttgaggta gacggccatg ccggcaatca gcccgccccc
     3121 gcccaccggg atgaacactg cgtcaatcgg ggcgctgtgc tgccgcagaa tctccatggc
     3181 gatggtgccc tggccggcga tcacggcggg atcgtcataa ggatgcacgt aggaagcacc
     3241 ggtctgccga gccagttgtt gcgcgtaggc ccaggcatcg tcgtaggtat cgccgtgcag
     3301 aatcgtttcc gcaccgtagg cacgtaccgc attgaccttg atttgcgggg ttggtgtcgg
     3361 catgacgatg gtggcacggc aacccagtcg gcgagccgcc agcgcgacac cctgggcatg
     3421 gttgccggca gatgcggtaa tgacgccggc agtcagtgct tcggcagaca accctgccat
     3481 cttgttgtac gcaccacgca gcttgaagga aaacacgggt tgcatatctt cgcgcttgag
     3541 caggacgcgg ttgttgatgc gtgcggacag tccggtggcg aattcgagcg gtgattcctg
     3601 tgcgacatcg tatacccgtg cggtcaggat ggcagtcagg taatcgaaat cttcgtgttt
     3661 catgtgcgcg tggcagggag ggccctgaaa taggtggtga caagacatga agattagcag
     3721 acctgggtgg atttgtggaa atcgtcattt atgggtatgc ttgcctgatg gtcttgtcga
     3781 tatttggccg acagacccga ttgaggatga acatggacca gaatgacatg aagcgtgcgg
     3841 tggcgcgggc cgccatcgat tttgttccac aggggataat tggtgtcggc accggatcga
     3901 ccgccaattt tttcattgat gagctagccg gtatcaaggg tcggtttgat ggtgcggttg
     3961 ccagttccga ggctacggca caacgactgc gcagtcacgg catcaaagta tttgaactca
     4021 acgaagtggg tgatctggac gtgtatgtcg atggcgccga cgagattacg cgccacatgc
     4081 acatgatcaa gggcgggggc ggagcactga cgcgcgaaaa gatcgttgca gcctgcagtc
     4141 gccagtttgt ctgcgtggcc gaccagacca agctggtcga tgtgctcggg cagtttccgc
     4201 tgccggtcga ggtgattccc atggcgcgca gctacgttgc gcgccaactg gtcaagatgg
     4261 gtgggcaacc caggctgcgc gaaggattcg tcacggataa cggcaacgtg attctggacg
     4321 tggtcgggtt aaacattctc gacccggttc gtatggaatc ggacatcaac cagatagtgg
     4381 gtgtggtgac caatggcctg ttcgcacggc gtggtgcaga tatcctgctg atgggtacgc
     4441 cgtcaggcgt ggagatgttg cgtcccgcct gatcctgcta cggatcattc gtccgtgcat
     4501 tcgtccgcgc tggaggacgg atgcgtcgga tcgccttcga tgcgccagcc ctcggtcgcc
     4561 cacaggccga gatcgatcag ccggcagcgc tcggaacaga acggacgcca ttgctgggtc
     4621 ggtgtccatt cgacttcctg ccggcaggtc gggcattgta cgacgcaggg ccggttgctc
     4681 atagctgaca aaagcacagc tggaattcga tatcctgctc gcagcgcgcc ggtaatccct
     4741 tgcctgcgtt gaggaaacga atgttgatgg cgtgacggtt ggcgctggct tccgggatcg
     4801 tctggctgtc aacaggtaac ccgatgcgca gcatgtggct gctccggcca ttgagcgcct
     4861 gctgatagta cccatggggc gcgagcaagg ccttgggtgc gccgttttcg cgtatgcgct
     4921 tgagcagcag gctgagcgcc tgatgtagcg gtagcatggg cgagatccag ttgacgaggt
     4981 cggccttgcg ttgtgccggg gacatgtgct gccagtaatg gaactggggc aggtcgaacc
     5041 cgttgacacc tcccggaatg ttggctcgct ggcggacgcc ggcaagccag tcgcagtcgc
     5101 gcagtggttg gtcgagccgg gcaggcatgg cctggagtgc cgccagcgcc tggtcgatga
     5161 aatctacgga tgattcgttg ccagaagaaa ccaccgcacc gtcggtgcgt tgacgcagtc
     5221 gcgtcagttc catctgcagt tcggagcgca tttcattgcg tgaacccagc tcgaacaggt
     5281 cgaaaatgcc aaacaacgct gtctggtgca agagcgggtc ttgttgccgg gccagataaa
     5341 gtacctgctg gaacagcctt tccagtttga gcagggtgcg cacgtgttcg ttaagaggaa
     5401 attcgtagac aatcacaggg cggctgtccg ctggttggtc atgaagggct attctgacac
     5461 gcgatgactg gtgctggcaa tcgacttgct ggcagcaagg cggtggcagc gttcgttcag
     5521 tgcgcgcacc tgccggtcaa gcgcatcggt gtcgccacgg ttgtcgatga tctcgtccgc
     5581 aagtgcgagc cggtcctgac gtccgatctg gctgcgcatg atttcgcgta ccatggattc
     5641 gtcgagcccg ctgcgctgca tggtgcgggc gatttgctgc gattcgtcgc agtcgatcac
     5701 caccacgtgc tgcatgagcg gcagataacg cgaatgcgcg tcgagcagcg gcacgaccag
     5761 tgcaatccag ggtgcgcgct gcgcctgggc gatttctgcc tgcgcggcac tgaaaatcat
     5821 cgggtgcagt atgtgttcca gttcgcgtct ggcctcgggt ctggaaaata tcagggcgcg
     5881 catgcgcggc cggtcgagcg ctccggtggt atcggcacag gtggcgccga aatgggcgag
     5941 cagtgctggc atggccgcac cacccgggcc ggtgagctgg tgcgcgatgc gatcggtgtc
     6001 gatcacgggt acgcccagcg ccatgagacg gtcagtgacg ctggtcttgc cgcatccgat
     6061 tccgccggtt acgccgacaa tcaggctcat catgccagac caagccaggc atggctgatc
     6121 gccgttcccc agaacaaggc aagcagaccg cctcccgcca gaaatgggcc gaaaggaatc
     6181 ggcgtcgccg cattctgcct gcgcagtgcg atcagggtca tgccgacgat ggcgccgatc
     6241 agcgaagacg ccaggatgac cagcggcaac atctgccagc caagccatgc gccgatggcg
     6301 gccagaagct tgaagtcgcc atagcccatg ccttcctttc cggtgaccag tttgaacagc
     6361 cagtagacag tccacagact gaggtagcca gcgatggcgc cggtgacggc gtccggcagc
     6421 gttgccagtg tgccgtgcag attgaacagc aagcccagcc agagcagtgg cagcgtaatg
     6481 cggtcaggca gcaaccgggt gtcgaggtcg atgaaggtca gcgcgatcag cgcccaaacc
     6541 aggcacaggg cggccagggt ctggagctgc atgccccagc ggacggccac cgcagcgctg
     6601 agcagtccgg tgagcagttc gaccagcggg tagcgcacgc caatgcgcgc agcacaggcc
     6661 gagcagcgtc cgcgcagcca caggtagctt agcagcggaa tgttttccac gatgcggatg
     6721 gcatggccgc agtgcggaca ggtggagcgc ggggtggaca ggttgaacgc cggcaactgc
     6781 ggcactgcgg tgccggcaag ctcggcgcac tgcgcctgcc attcacgctc aagcatgacc
     6841 ggcaggcggt agatcaccac gttgagaaaa ctgcccacta ccagccccag tacggcgcac
     6901 agccaggcaa cagccgccgg gtaggcctgc agcaggctca gagtgtcagc caacgacgct
     6961 tcccatcttg aagatcggca ggtacatggc gacgaccatg ctgccgatca gtacgccgag
     7021 gatgaccatg atcagcggct ccatcaggct ggacagtgca gcgacggcat catcgacttc
     7081 ggcttcgaag aagtcggcca ccttggagag catggagtcg agcgctccgg attcctcgcc
     7141 gatggccacc atctgttgca ccatgttggg aaaaatcccc gagttttgca ttgcaaccgt
     7201 caggctggtt ccggtactga cttcgctgcg tatcttttgc gtggcgttgt ggaacacgat
     7261 gttgcctgcg gcgccggcca ccgaactcag tgcgtcgacg agcggcacgc cggcagcaaa
     7321 catggtcgcg agcgtgcgcg tccagcgtgc tatggccgct ttctccagca ccggaccgaa
     7381 aatcggcagg cgcagcatca gtcggtccat gaattcctgc atcggtcgcg atcgtttcca
     7441 ggtgtagaaa aagaaccaga tggcgccgcc cagaccgccg aagatcgccc accagtaggc
     7501 gacaaagaaa tcggaaatgc tcatcatgat cagcgttggc gtcggcaaat tggcgccgaa
     7561 gctggtgaac agctgcttga acgcgggtac gacgaacagc atgatgatcg ctgtgataat
     7621 gaaggcaacg atgatgatcg agaccgggta gaacagcgcg gatttgatct tgcccttgat
     7681 ggccagcgtt ttttcctggt aagtggcaat gcgctcaagc acctggtcga ggataccagc
     7741 ctgttcgccg gcattgacca gattgacgta cagggtatcg aaatacagtg gatgcttgga
     7801 aaaagactga ctcaggctgt tgccgctttc gatatcagac ttgatgtcga tgagcagttt
     7861 ctgcacggat ggattggcat ggcctttggc gacgatgtcg aatgcctgca gcaggggcac
     7921 cccggctttc atcatggtgg caagctggcg ggtgaacatg gccacatcct tgccgctgat
     7981 gcgtttgcca cgggcaaagg ccgccttttt gcgtatcttg cgaatgttga tgccctgtcg
     8041 gcgcagtgtg gtgcgtacca cggcctcgct ggccgcaatc agttcgccct gggctttctt
     8101 gccggccttg tcggtgcctt cccagctgaa ggtgacgtcc ttgctggcgg ccttggcggg
     8161 taatttcgca actgccatat cgtattctcc gggttgctgt gcgccggtcc gcgcggaact
     8221 ggctccgggt ggattctgcc agaggtggcg ggccgtgtca ttcgttggtg acggattcga
     8281 cttcttccag cgaggtcagc cccgccttga ccttgttgag tcccgagcga cgcaggtcgt
     8341 tgacgccttc gcgacgcgcc tggtcggcga tatcgatggc attgccgttg ctcatgatga
     8401 tgcgtgtcat cgcatcgctg atcggcatga cctggtagat gcccacgcgt cccttgtagc
     8461 cggtgttctt gcacagttcg cagcccaccg catggtaggc ttgccaactg ccgtcgagtt
     8521 cggatttgtc gaagccggcg cgcagtagcg cctcatgggg gatatccacg ggctgtttgc
     8581 actggcacag gcgtcgtgcc aggcgctggg cggtgatgag aatgacgctg gaggcgatgt
     8641 tgaacggggc aatgcccatg ttcatcagcc gtgtgagcgt ggaaggcgca tcgttggtgt
     8701 gcagcgtcga taacacaagg tgaccggtct gggcggcctt gatggcgatt tccgaagttt
     8761 cgaggtcgcg gatttcgccc accatgatca catcgggatc ctggcgcaga aacgatttga
     8821 gcgccgcagc gaaggtcagc ccggcttttt cgttgacgtt gacctggttg atgccggcca
     8881 ggttgatttc ggccggatcc tcggcggtcg aaatgttggt accaacctcg ttgagcatgt
     8941 tcaggcaggt gtagagtgac acggtcttgc cgctgccggt
//
LOCUS       DAIANL010000144        18728 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_388625, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000144 DAIANL010000000
VERSION     DAIANL010000144.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 18728)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18728)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18728
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388625"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3442 a         5488 c         6275 g         3523 t
ORIGIN      
        1 gatgccatcg tgggacggcg gcagccggta gatcggctgg ctggaaatcg tgctgataaa
       61 aatgcccctg actttgtgga gtcaggggca ttgtacgctt gaatcagtta aaccatcaac
      121 acgttaactg aacggcttgg ccttcggggg ccttttcgtt gtgaaggcaa catgggtacc
      181 aggggagtga gcacctactg ccctttcgga tagccggtgc acggacctgg agcaagtgtt
      241 ccaggtacac atgaaaacac cggcacatgc caattggcgg catggggtat gcaggttgcg
      301 ctgagtcatt gcagtgtatt cataacgccg gctaacgggg tgaaacagca ctgcactacc
      361 tgatgtctcg tcggcaagcc gtatctggct ggcagcgggt gcgggcgaac cgaaccaggc
      421 aacacggtct gaacctgcag atttaccagc ggatgcgggc cagcaatgct gccaggaggg
      481 cgctgattgc gaaaccgtgc agcgctgcgt gcatgcctag cgtcgcgcag accagcgggc
      541 caaggaagct ggccagtgcg agcgtgccaa acagcaggcc gaatgccgac gcggcgcagt
      601 tgtcgtcaga gacatgtgcg gccaatgccg gcagtttgcc ggccatgcca ccaaacgcca
      661 gcccgatgag caacggaaac ggtatgaaca cccggtcatc cgcgccgtgc agtatggcaa
      721 gcagcaaggc tgcagccagc gcgttgaggg tcagcacggt gttgcgggcg aaatgggcgg
      781 aacgcagatc ggccagtacc ccgaatccca gccggcccag cccgttgcca accgccatgg
      841 aagtcaccgc agccagcgag aatgcagttc cacagccgag ttcatggaca agatgccctg
      901 tcgtggcgaa tgccgccgag cccagcgggg cagccagcaa tatcagcagg agtgcctggc
      961 gcagcgggcg gtggctgagc gtggtccaca ggccgctgcg ggtggcgccg gctgattttt
     1021 gcagcggaat atccggcaat ccggcccagg ctgaccaacc cagaaaggca taggcgccca
     1081 gcagtccggt gagcgcggtg cgccagccga aatgcccgtt gagccattcg aataacggtg
     1141 cactgacgac ggaagaaacg ccgtacagcg ccagcagagc ggcgcccggc gctccctgct
     1201 gttccgggta gagccgggca acaatggcaa tgcaggccgc tgtagacaga ccggcgccga
     1261 tccccagggc caggatgcca tagccgagca tgaacggcac cggtgtcgcg aatgccaggc
     1321 ctgtaccgcc accgatcgtg aaacagccga gtgccaccgc caacatggtt ttgggcgaca
     1381 tccgatgcag gccatgtccg gccgaaacca ctgctggcag caaggccagc ggcgtcaatg
     1441 agaacgtcag gtccagggtg gcctgagacc aaccgctttg agcatggatg gccggtgaaa
     1501 ttgcgcccca tgaataaatg agcccgaaaa tgaactggaa cggcgccagc aataccatgg
     1561 gcagcccgtt cctgcgtaat ggtgtgttgt gcaccggcgt ggtcatgtca cacaccaggg
     1621 atgcaccgcg gcgggccacg cgcaggtggc caggacatgt gcgtcgctcg tgctgctccg
     1681 atgatcggcg ttacggccgt attgacagca acacggcatg gcaggtccgg attcggggag
     1741 cgtgagtgca actccggaca tggcagtgaa atcagacggg acggcggtca tggtagaaat
     1801 tgcatagtgg ctggatattc aagcagacca aaggggattg gaggtattct agacgttggc
     1861 gcgcggcgaa cttgtcgctg gcggaaccga atccttgaat gggagtgaac ttgaattcgc
     1921 actcaggcac gcagcattcc gcttctggat gggaacacct gctcaggcag catttcatcc
     1981 ccggtcgggt cgaactgcat ggcgacagcc ctggcggcca tctgcagcgt cagatgcgca
     2041 tcaatcgtct gtggctggcg cagctgtcgc tctacccgca gatgattgcg catatgcctc
     2101 accatcttga tgcgttggcg cccgaagacc gggcatcagt gatcgcgcac gtggtcatcg
     2161 acggtagtgg ctttattgat cagggtggcg tatgtctgcc ttttgcagcc ggggacattt
     2221 cgtttcgcaa tctcgccgaa ccatcacgcg tggtgttcga tacgccaggc aacgtttacg
     2281 ccatacggtt accggccaca gtcatgcctg caccgcgacc agatcagccc gggcgccgcg
     2341 gcctcgcgcc acgggttgcg cacacggcga acctgccgtc cgaagcggtg cggcgactgc
     2401 tgtccgctgt ggcctggcat ggaacgccgg gtatgtccgc gttttgcgtt gcatccgccc
     2461 tgccctggtt gtttgctgcc gcatatcatg gggaggacac ggcgccaacg gcgagaggcg
     2521 ccagcaacgc cttgcgctgg caacaggtgc tcgattacat cgaacatcac ctgttcgacg
     2581 ccgacggagt gtctgtcctg gcgtgtgccc agacgatcgg cgtatcgcag cgctatgtgc
     2641 atcatctgtt tgcccagcgc gggcagcgtt tttccaaaac cgtgcagcaa cggcggcttg
     2701 aagccgcgca gacgatgctg caaaccccgg ttttccggac acagtcgatc gcttcggttg
     2761 cctatcaatg cgggttccgg gaaccggccc atttcagccg gctgttccgc cagcgcttcg
     2821 gcatgtcgcc gcgcgaatgc cgcattcgca gcggactgga tcgtgtttct gcaacggctg
     2881 gatgatgcag cttgtaatcg atatgttcgg tgcggaggtt agctcatgac acaggaaatt
     2941 gttctgcttt ccgaactggt gtgcccccgg tgcgggcacc gcaaacagga gcgcatgccg
     3001 acagatgcgt gtcagtggtt ttacgaatgc gaggcgtgcc acgccctgtt gcggccaaaa
     3061 cctggcgact gctgcgtgtt ctgctcctgg ggcagtgtgc catgcccgcc ggtacagcaa
     3121 caggggcgga tgaggaactg ctgccaatga tggacgcggt tccgtgtggc aatcgtgtcg
     3181 ccaaaacgca tgcggcttca cctgccacac gccatttcag cccgagatgt ccagaaccag
     3241 ctccaggtga tggcagcttg gctgaaagcc cttgcgcaga tatacgcggt gcgctgcgtg
     3301 gcgagcataa ccggtatcaa ggtgaacggc cgtgcaacgc tgttcgcgtg cgtgatcgat
     3361 gagccagtcg agcaccgcgc tgccataacc cctgccccgt tcggcctcca gtgtggcaaa
     3421 gtcatcgatg tagagcactt tgccccaggc aagaaattcc gtgaggcgaa aaccggcgag
     3481 gctctttacc cgtccctcat ggctcagcgc cagaagccgg tatccctgct gttgctgccg
     3541 gcgtatccgg gacaggaatt cgtgttgcac gagatgcggg cgcagggcat gcagtaccgg
     3601 aaaacaggcc tcgacctgtg catcggtgct tgcgatatca agcgcatgat ccatcaaagg
     3661 gttgagtgcc actgccggaa cctgtcgttc atgaggactg gcggcggcag cggtatacgg
     3721 cgtgccatcg cggtgtgtaa atgaccagcg gccaccgtca tccagtgtgg cgagcaggtc
     3781 gtcttcgggg taactggcgc ggtctccggg gtagcggtcg ccgatttcca gataggtgac
     3841 gggtagccgc gagcgattga tgagctggtg accaaggccc gttccggcgg ggaatcctgc
     3901 gcacatgtgc ggtgacagca gcatttcttc gctaccgtaa cgcagggtcg gtgttccttc
     3961 cagcacatag atgaattcat cctgctggtc atgcgcatgc atcagcgcgg actgggcgcc
     4021 cggttcgagc cgcgtcaggt tgacgccaaa atgtgtcagg ccgaacggct cgcccaaggg
     4081 gtgtttgagc cgacccagca tgcgccgggc aaactcgggg ggatagctgg atgcctgggc
     4141 gcgtggaacg gcttcgctgg ccagcagggc aatgcgcgga ggttgttcgt gcatggtggt
     4201 tccttgcgac acgagtttct gtcggtaatc gtggcaatca tgcccggttt gcgggggggc
     4261 tgcgtcgtgc gcgccagaag gcgctgccga gcagcaatgc ggccagcgcc agcacgggtg
     4321 tatcgccaaa gcgcacatac ggcgtggtgc cggtatagcc gcgtacctgg ccgtcgagcg
     4381 ttgcggcagt gaataacggc agtgtggccg ccaaccggcc atccggtgtg atgatggcgg
     4441 tcaggccggt attggtggca cgcagcatgt agcgtcctgt ttccagtgcc cgtgcctgcg
     4501 acatctgcgc atgctgccat ggcgcggcgc tgcggccgaa ccaggcgtcg ttcgtgatat
     4561 tgatcaacag gctggcttgc ggcaaagcgt gaatgatttc gttgccgaac acatcttcat
     4621 aacagatgtc cggagcaaca cggaggccat cgacacgcag tggtggctga aaggcgctgc
     4681 cgcgggaaaa gtcggccatg ggcacctgca gcaattgcaa cagtgatccg aacagcgatt
     4741 gcagcggcac atactcgcca aagggcacca ggtgatgttt gcggtagatt tgcagtggcg
     4801 aggtgcccag gctgtacagg ctgttgaagt aatgcccctg ccggtcgcgc tcggggatgc
     4861 cgacgaggat gtcggcctgg cgcatgcgag cgctatcggc cagcacctgc agatacaccg
     4921 gcgggatctg gtcgtacaat aacggaaatg ccgtttccgg cagtaccacc aattgggcat
     4981 ggctggcgag caccatgcgc tcatagcgtt cgagtgtgct ggtgaggtgt tccggttgcc
     5041 atttgacatc ctgggcaata tcgccctgca gcagcgaaac cgtcaacggc gcggtgtccg
     5101 ggtgcgtcca gtgcacctgc tgcagtccca gtccggcaat ccataacgca accagtgcgg
     5161 agagggaaag acgcgtgggt gcctgcaatg cggcagcact ccatgccgtg agcagcgaaa
     5221 gcaggaacac gccaccgatc gggtcataac cggccagcgg cgaggccggc acctgggaat
     5281 acccgagcag cagccagggg aagccggtaa acacccagct gcgcacccat tcctgcagaa
     5341 cccagccggc ggcgagcagc aacggtgccc gccagaaagt gcgtgcggcg agccaaccag
     5401 ccagcgcggg aaacagcgcc agaaacgcac tgaacagagc aacggccagc gtggccagcc
     5461 acgcactcat gccgccatac tcgtgcagac tggtgtagag ccagctgatg ccgctggcaa
     5521 aatagcccat gccatagcac cagcccagcc aggccccccg ggcggggtat cgccgccaca
     5581 gatgaaacag cagcgcaagg ctcagcagcg gcaaccacca gagcccggtc ggcgcaaagc
     5641 cgaacacaca gaccgcaccg gccaggatgg ccagcagcgt cgtcacgacc ggtgaatcag
     5701 cgcgcagcgg caaagtccag catccgttgg atcggtcgac gcgccagtgg aatcagctcg
     5761 gcatccagtt cgatttcgtt gctgcctgtt tcgaggacct gtgccagatt gcgcaggccg
     5821 ttcatggcca tccatgggca gtgggcgcaa cttttacacg tggcgccggt gccggcgctg
     5881 ggggcttcaa gcagcagttt tcccggtgcg gccaggcgca tcttgtgaaa aatgccgttg
     5941 tcggtggcca cgatgaacgc agggttgggc agattacgca cggcctggat cagttgtgtg
     6001 gtcgagccta ccacatcagc ctgtgcaatc acgccggctg gcgactcggg gtgtaccagt
     6061 actgccgctt cgggatgcgc ggcgcgcagg gcttccagtc cctgtgtcct gaacgcctca
     6121 tgcacgacgc aagcgccggg ccagatcaac atgtccgcac cggtcaccga ctgtacccag
     6181 gcgccaagat ggcggtcggg cgcccacagg aggctgtccc cctgtgcatg caggtgctgc
     6241 acgatgcgaa ccgcgatccc cgaagtgacc acccagtcgg cgcgggcctt gacccgggcg
     6301 gaggtgttgg aatagacgac caccttgcgc tcgggatgtt cgtcgcagaa acgcgagaag
     6361 gcttcgggtt cgcacgacag gtctagcgag cattccgcat gcaggtccgg catcaacacg
     6421 cgtttttccg ggttgagcag tttcgcggtt tcgcccatga agcgcactcc ggccacgacc
     6481 agtgtccgcg ccgggtgggc atggccgaac cgtgccattt cgagcgaatc ggccacgcat
     6541 ccgcccgtgt cttcggcgag ttcctgcagg tcggcactgg tgtaatagtg cgcaatgagc
     6601 acagcgtcct gttgtttgag caggcggcgg atgcgatcga gcaatgcggc acgctcaccg
     6661 ggttcaagca cctcttcttc gacgcttgga gcgatgatgg gcggaatggc gggtaaatgg
     6721 atgggcgggg tcagggtggc ggaagacatg gtgtactctc gctagcggta gtattgcagg
     6781 gtgccgggtt gttgcagacg gtacagtcgg gccggtttgc gctggccgct gctgcggaca
     6841 tgaccggttt cctcgaccaa gccgagcgac aggatgtgtt tgcggaaatt gcgtttgtcg
     6901 agcgcttcgc cacggatgat ttcgtgtact ttttgcagct cgcccaaggt aaagggttcc
     6961 gtcatcaggc tgaacgccac ggtggagtat tcgagcttgg ctcgcaggcg ctgatgtgcc
     7021 agcgcgatga tgtcgccgtg atcgaatgcc agtgatggca gttcactgag caggaaccag
     7081 ccggtttcgc gggcgtccga gccggccgcc ggggcgagca tttcggaggg cgccagcgca
     7141 aaataggcta cgctgatcac acgctcgcgc gggtcgcgat ccggttggcc gaaagtgtac
     7201 aactgttcga gataaacgcc gtggatgccg gtttcttccg ccagctcgcg cagcgcgccc
     7261 tcttccaggc tttcatccgg ttcaacgaaa ccgccgggca gcgcccattc gttttcgaaa
     7321 ggcggcgctc cgcgccgtac caacagtacg gccagccggt cgtctcgcac cgtgaacaga
     7381 accgtatcgg tggtgaccgc aggatgagga tagggatagc agtacggcat gtcggcggcc
     7441 cgcatgaagt gtaaaaagta cacctcaatg ataggttcag gcgaaattca tgtcaatgaa
     7501 agtttgcgcc gttggacgtt cgggatgacg gacgtcggaa aaccggaacg ttcgcaccgc
     7561 ggaagtacga tcagggttgc gttcccggcg cgtcaatttg tatgatgcag gtcttgtctg
     7621 cctctcctgc cccgatgtca ctgatcgaat atctgcacct gctgctcgaa gtcatgctgc
     7681 cgatggcatt gctggtggcg gctggcgcat ggtggcgtcg cagtctgggt gaagacggcg
     7741 tgcgtgccgt gcgtggttac atcagcagca ttaccatcca cctgttcgcg ccggccctgc
     7801 tgttcgctgc agcggcaacg gcccgactcg accgcgaatt gctgtcggtg ccattgctgg
     7861 tcggcagtgg tgtgttattg tcgggtgcgc tgctggtcgt gctgttgcgc tggctgaccc
     7921 gccgtgccca gttaccgcgc acttcgctgg ccgcgttgat gctgtgcggc atgtttggca
     7981 atgtcctgtt catgggttat ccgctgctgc attacctgta tggcacccca ggggcgcagt
     8041 atgcggcatt ttccgacatg ggcgcgtcca cgccgctgct gtggagcctt ggcgtgtggg
     8101 tggccactca catcggccag caggtgcgtg acccgggcca tccactggtc aattggctca
     8161 agttgccgcc ggtgtggtca tttggtgccg ggctgctgct tgggcaaagt ggtctgccga
     8221 tcgatccgct ggtgcatgct gcgcggttca tcggccaacc tacggtaccg gtcatgctgc
     8281 tcatgctcgg cctgtcggtg ccatggcgcc ggcttgcacc cacgcgctgg gtgggaatga
     8341 ttgtcggcta caaactgctg gtgctgccgg cgctggtgtg gctgatggcg cgggcgctgt
     8401 atgggccatt gcaccaggcc caggtcggcg cagtgatcga aagtggtgtg ccgaccatgc
     8461 tgatggccct ggggctggcc gatcgttacc ggctcgatgt cgaaaccaca gcactggcta
     8521 tcgcttggag tacaattctg ttcgtgttca gtctgccatg gtggctgggc tggctgcatt
     8581 aaaggaaaac acatgagcct aatcttgccg ccaggaatga cgcgcgccac cccactggag
     8641 aagcaggtca cgctcgtcac ccgttcgggt gagcgcctaa gcatgccgtt cacgcttcag
     8701 gacgagctcg gacgcgacag cgctgccgcc tggatcaacg aacttaacgc aaccattcgc
     8761 aacaagctca atgagccggt gctgggtgag cttgccggtc tcgccgctga catcgagaca
     8821 ctcaaacgca agattctgta catcgccggg tttcacgatt cgatgtttgg caccttcaat
     8881 cgccagagta ccctgccgga aaacgaacgc gccgaattca tcgagctgtt cctgctggcg
     8941 gtcgccagcg tgattcccgg aatgcgtatc gagatcaatc tgctgtccgg gcgggttacc
     9001 agcgacggaa actgagtcgt gcacatacat attcttggaa tttgcgggac gttcatgggc
     9061 ggcgtcgcgg ccctggcgcg cgcagccggt caccaggtga ccggctgcga cagtcaggtc
     9121 tacccgccga tgtcggacca gttgcggttg ttgcagatcg acctgatcga agggtttggc
     9181 gaagaacagc ttcgactgca gccggacctg ttcgtcatcg gcaatgtggt gagccgcggc
     9241 aacccgctca tggaggccat acttgaccgt ggcctgccct acatctccgg gccacaatgg
     9301 cttgcggagc acatcctgcg cgacaagtgg gtactcgctg ttgccggtac ccacggcaag
     9361 accaccacca gcgcgctgct ggcgtggata ctcgatgtcg cggggctcaa tcccggattt
     9421 ctcatcggtg gtgtggcgaa tgattttccg gtttcggcac ggctcacgga ttcgccgttt
     9481 ttcgtcatcg aagctgacga gtatgacacg gctttcttcg acaagcggtc caaatttgtg
     9541 cattaccggc cacgtacgtt gatcctcaac aatctggaat tcgatcacgc agacatcttc
     9601 gccgatctgg cggccattga aacgcaattt catcatctgg tgcgcaccgt tccgcgtcag
     9661 gggcggatat tggcgcatga cgagcccagc ctgcgccggg tgatcgcgcg cggctgctgg
     9721 acgccggtcg agtggctcgg tgcggcggat ggctggcatg ctgcgggcga tcctgccgcg
     9781 ttcgaggtgc gtctgggtga ggtgctgcag accggggtgc actgggggct gcttggcgag
     9841 cacaacatgg ccaacgctct ggcggcgatt gcggcagccc ggcatgccgg cgtgccgccg
     9901 caggtgtcgg acgaagcact ggcccggttt tccggcatcc gtcgccggct cgaggtccgt
     9961 ggcacggccg atggtgtgac ggtatatgac gattttgctc atcatccgac cgcgattgcc
    10021 gctacgctgg gcggtttgcg gcgtaaggtt ggcaccgcgc gcatccttgc cgtgctcgat
    10081 ccgcgttcca ataccatgaa gcagggcgcg ctcaaggatg cgctgccagc cagtctggac
    10141 gaggccgacc tggtgttctg ccatgcggcc gggttggcct gggatgccag ggcggtattt
    10201 gccggcatgg gcgccatgac acaggtgttc gatgccatcg aaccgctggc tgcggcggtg
    10261 gtggccagtg cccagccggg tgaccacgtg ctggtgatga gcaacggtgg tttcggcaac
    10321 attcacgaac gcatcctgca agggttgcgc gcgcgggcgt ctgcagcagg cgggtctgcc
    10381 ggctaaaccg ggctggacgg gatgcgggat tcgtcccaga tctcgacggc gcgacgccag
    10441 aaggcttcca cactgccgac gggttccatg gtctggccga gtgcgaccat tgcctgttgc
    10501 aggtcggtga gcggatcgtg ttcgccgacc ggggctgcgc gggtttgctt ggacagtttc
    10561 tgaccgagcg tgttgtacac cagggggaga tgaccgtagc gtaccggggg caagccaagc
    10621 aggcgttgca ggtggatttg tcggccggtc gaatcgagca ggtcggcacc acgcaccacc
    10681 tcggtaatgc cgctggcggc atcgtccacg actacggcca gctggtaagc aaaatagccg
    10741 tcggcgcgca ggatgacgaa atcgccgccg cactggcgtg ggacctgttg ttgcatgcca
    10801 cagtagcggt cggcgaaggt gatgggccgg tcgtcaacac gcaccctgac gctgtgtcgg
    10861 cctgaaggcg cgagtgcgcg ctcactgcag ccgccgcagc atgaatcggt gccgtcctgt
    10921 gcccgttcgc ggcggctgca ggtgcaggcg taggtccagc cgccgttgcg cagcatctgc
    10981 agcgcatcgt ggtacgcgtc cagacgttcc gactgacgga tcaccgggcc gtcccaggtg
    11041 aaacccaagc gttcgagctg gcgcagaatg gtggcttcag ccgccggcga acaacgcggc
    11101 gtgtcgaggt cttccatgcg cagcagccag cgccctgccc ggctacgcgc ggacaggcag
    11161 ctgcccagcg ctgcgaccag agagccggcg tgcagcgcgc cagtgggtga tggggcgaaa
    11221 cgcccgatga cggcgagatg cgaattcatg tttcccaagc gggcagtgta acgcatagcg
    11281 ggtggtcacg ggtaccactc gcacgggcac cgcgtcaggt gcggcgcggg tgaccggaga
    11341 tgacgtgatg gtagaactcc gacatctgcc gcgccatgct gcctgctgtc cacgatagcg
    11401 catgttcgcg cgcttctgtg ctcaactggc gacgcaattc ggtgtcagtg agtacgtgcg
    11461 ccagcgtttc ggcaaacgcc accggattgt ccgtggctac ccggcagccc ttgccggcag
    11521 cgaggatgtc gcgcgtgccc atgatggcgg tcgataccac aggcaggccg agtgccatgg
    11581 cttcgagcag taccagtcct tgcgtttcgg tgcgcgaggc gaacacgaag gcatcaccgg
    11641 cgaggtaaca ggaaggcagt tcggtcgagc gttcgaggta gccgacaaac agcacgtttt
    11701 cgctcaggtt gagcgcatct accatctgct tgagccgcgc ttcggctggc ccttctccag
    11761 cgatgatcaa caatgcatcg ggcagattgt gtcgcagctg cgacagcatg agcagcagga
    11821 attcgatgtt tttttcgaag gcgacgcgcc cgacatagac gatgaccggg cggtgcgggt
    11881 cgatgcggta tttctgacga aaagcgtgcc ggtcgccacc gcgaaaatcc gacaggtcga
    11941 tgccggtggg aatgatgcgg ctgagtgtcg atacgccgta acggcgcagc gcatcatcca
    12001 tcgcctgcga aggcaggatc aggccgtcga tctgggcgca ctggcggcgg gaaaaatggc
    12061 gtgccacata gcgcatcagc gaacggggca ggaacgggat atagtggtac aggtactctt
    12121 caaagaaggt gtggtaggtt tccacacagg gaatgccgaa ctggcgtgcc agtttgagac
    12181 cggtataatg ggcctggaat ggggtatgca cgtgtaccag gtcgaactgc cggccttcga
    12241 gctggcgcga cagcgcgcgc agttgtgcgg tcgacatgaa gcggtcttcg gggtcgagcc
    12301 acactttgcg gccattgatg cgcaggatgt cggcttcccc ggtttcattc atctggtagg
    12361 ccggcgcaac cagcgtaacg tcgtgtccgt gcgccttgag ctcgcgccgc aaggtcatga
    12421 tcgaggtgga caccccgttc acgcgcggaa aatagacatc ggataccatc aaaatgttca
    12481 ttcagccaat cctgcaacca agttgtattc gatttgtaac aaaatcaggt gacgtactga
    12541 tgacagctgc tgacatgcat gctgccctgc cctgcccggc atcatcgtcc ggcaggcggg
    12601 caccagacgg agcgccatgc tgcatctgac cttgcgccaa ctgcaggtgt tcgacgcggt
    12661 ggcgcgccac ctcagcttct cgcgcgccgc gcgcgaattg cacctcaccc agccggcagt
    12721 gtcgatgcag gtgcgcctgc ttgaggaaag cgtgggcttg cccctgttcg agcagctcgg
    12781 caagcggatc tatctgaccg aggccggacg cgagatgcag cagtacggcc agatcatcgc
    12841 cacccagttg cgtgaagctg aagcggtgtt cgagcagatg aaaggccttg aacatggtga
    12901 actgcgcatc gcggtggctt cgacggcaaa ctattttgcc acgcagttgc tgtcgcggtt
    12961 ctgtcagtgc catccgggcg tcgaagtgca tctcgaagtg agcaaccgtg cggatctgct
    13021 gctggcgctg gaacagaacc gcatcgacct ggtgatcatg ggacagccgc ccgaggggta
    13081 cgacctggag gcggcttcgt tcatggaaaa tccgttggtc ctgatcgctt ctccgcgcca
    13141 cgcgctggcc ggccggcatg gcatcagcgt ggaagtgctg gcaaacgaaa cgctgatcct
    13201 gcgtgaaccg ggttcgggta cgcgcggtgc caccgaacgg tttttccagt cgcacggcct
    13261 ggtgctgcgc agtgggctgg tcatgaacac caatgaagcg atcaagcagg ccgtgcaggc
    13321 ggacatgggg ctggcggtgg tgtcgttgca tacggtgacg ctggaattgc aaacgcagca
    13381 actggtcgtg ctcgacgtgc tggggtttcc gctcaggcgc aactggtttg tcgtgcatcg
    13441 ccggctcaag cggctgtctc cggtggcgca ggcattcaag acgttcctgc ttgccgaggg
    13501 gcgtcaggtg gcacaactgt tgcagccggc gcctgtcccg ggcgctgccg gttaggccgt
    13561 gcccgggaca ggccccggtg ggccgcgtgc accgcatcgc ctgccgcgcg ccggccggtc
    13621 cgcgtttcgt taacgggggt cgcctggacg gccgatgctg cgcgcgtgct ggctgcgtac
    13681 gcgggcgatg agttcatcga gcgtcttgaa cagcgcctgc tcgctgccgg tcatggcgac
    13741 cgaggtgaca tagcggccgt cgacaacgaa acttggcacc ccggtgatct gcgaatcgcg
    13801 cgtgagctgc gctgcctgcg tcaccttggt ctgcacggcg aacgagtggt acgtgttggc
    13861 gaaggtttgc gggttcattc ccattcgtcc cgcccaggcg aacaggctga gttccgagga
    13921 cgaatccagc ccatcgaggt ggatggcgtt gaacaggtcg tcatggtatt tgtcggccac
    13981 acccatcagt ttcagggtgt accaggcgcg cgcttgcggc gcccaggttt cgttgagcac
    14041 ggctggaatg cgccggatcg ccacatcgcg cggcagtcca tggatccagt cgttgagcgc
    14101 cggctcgagt tcgaagcagt gcgggcagcg gtaccagaaa aattccgcca cttcgatgcg
    14161 gttgccgctg gtggtcagca acggtacggc cagttgcacg taatccttgc cgggttcggc
    14221 tgcgcgagag gccggcggga tggccaggga gatgatcagc agacaggcgg tcatgaacag
    14281 gtgggacaaa cgcatgcgat tttctccgta cggtggatta actgtcgctt gaaacgggcg
    14341 catcgcaccc cccgcgctgt tgcacgcccg tccggtttcg ctcagacctg cgtccgtgag
    14401 gccgcgtcgt tgccggccgg tctgcaccgg aacccgtgag gcgaattcaa ccgtgtgacg
    14461 ggcgcgcgca gcctggatgc tgatcgccac atgatctgcg ccctgcaact gatcgtgctt
    14521 cggcaaggta tccaaacggg ttgccgggtc gcgtcagtgg ccggccgggg ttgcggctgt
    14581 gccggcagaa accggtgtgg caaggatgcc gttctgttgc agcgatgtct gcactctggc
    14641 gagatcggtg gcgttgaacg gtccgaggcg cacccggtgg cgcgtgccct tgtcgggcag
    14701 gtcgacggtc tggatcgagg cttcgaagcc catcagggcc agccgggcct tcaggtcatc
    14761 ggcctggttt gcatctgcaa acgagcccag ttgcagccag gtggcgggcc tggctgccgg
    14821 cgtcgggacg gaaggtgctg caggcgtggg tgtggccgga ctggccgcat tgttgcccgg
    14881 caggatatgg tagaaatcat aatggctgtc gaccggagcg gcctgggttg ccggatcgat
    14941 aatgggcttg ctgccagggg tgattgccgg tgctgccgtg ctgggggcgg acacgctgcg
    15001 ctcggtgaac gggttgttgc gccccagcca caaggcgatg agcacagcgc cacccaggcc
    15061 gaccagcagg ccgatcatga tgccggacca gatgcctcca gaccgggtgg aagcgctgtc
    15121 gcgcgaggaa gatgctcgag ccatggggtt ctcctacatg tgatccggac tggaaatgcc
    15181 cagcagcgtc agcccgttgc gcagcaccgt acgtgtggcg gcaatcagcg ccagtcgggc
    15241 agtgcgcagc gtttcgtctt ccagcaggaa ttgttcggca ttgtagtagg cgtgcagtgc
    15301 ggcagcaatt tccttgaggg cgtaggccag cagatgcggt gacagctcgc gggccgcagc
    15361 actgatgaca ccgggatact cggccagccg ctgcagcagt tctcgctcgt gggcgctgac
    15421 caatggcgag cagtccgcac ggacgagttg ttccacatcg ccgccccact gggtgagcac
    15481 gctgctgatg cgcgcatgcg catactggat gtaatacacc ggattgtcat tggagcgcga
    15541 tttggccagg tcgaggtcga aatcgaggtg ttggtcggat ttgcgcagaa tatagaaaaa
    15601 ccgggcggcg tcgttgccga cttcgtcacg cagttcgcgt agcgtgacga attcgcccga
    15661 gcgggtggac atggcggctt tctggccatt gcggtagagc acggcgaact gcaccagcga
    15721 cacggtgagt tggtcaggat tcttgcccag ggcttcgagt gcgccgcgca cgcgcggaat
    15781 gtatccgtga tggtctgcgc cccagatgtc gatcagccag ctgaaaccgc gttcgaattt
    15841 gtgcaggtgg taggcgatgt cggaagcgaa ataggtgtac agaccgtttt cgcgttgtac
    15901 gacacggtct ttttcgtcac cgtagcgcgt cgagcggaac cagagcgcac cgtcctgttc
    15961 gtagaggtgg ccgtgttcct gcaacacctt cagcgtgcgt tgcaccacgc cctgatcgaa
    16021 cagcgtctgt tcggaaaacc actggtcgaa ttcgacgccg aattcgtgca agtcgttacg
    16081 gcaatcgttg agctgttcgt taagtgcgaa ggcatggaaa tacaggtagc cgtcacccag
    16141 tgtctgcttg gcctgtgcga tcagatggtc gaggtgggcg tcggcttcag tggccgcatc
    16201 gggagcggtc gcatacagcg gcgcggcagc gtggcgatag cgggtgccgt gctggctgaa
    16261 aatggcttgc gccatctggc ggacgtaacc accctggtag gcgttggcgg gaaacaccag
    16321 cgtttcgccg cagcattcga ggtagcgcag ccaggtggac agggcgagga tgtccatctg
    16381 tcgcccggcg tcgttgacat aatattcgcg cgatacccgg taacccgagg cggcaagcag
    16441 gttggcaaga ctcgcgccgt aggctgcacc gcggccgtgg ccaacgtgca gtgggccggt
    16501 gggattggcg gaaacgaatt cgacttgtac cgaccgcccc tcgccctggc ggttgtggcc
    16561 gaagcgctcg ccgtgttcca ggacatgcgg cacgatctgt tgccaggcaa gcgggtgcag
    16621 aaatacattg atgaacccgg cgccggcaat ttcggtacgg gccaccagcg ccgacggcgg
    16681 cagtgccgcc tgcagttgca gtgccagtgt gcgtggctgt acgcgcagca gcttggacag
    16741 tctgagcgcc acactacagg cataatcacc gtgggcggct tcgcgtgggc gttcgatctc
    16801 caccagcgcg ggatcggcat cggcggccac ttcccgcagt gcaaaatgaa acagcgaggc
    16861 aaggtgggct gggatatcga tggtgttcgt caagatgcaa tccgggtacg agatggcagc
    16921 attatagcca atgccggcag ggaaggacaa aacgattcaa atctgctgcg ggtcgacatc
    16981 aagccgccag cgaacttcac gcacgcgcaa cggttcgagc cggcgcatcc attcggccag
    17041 ccagcgttgc agcgtcggac ggtgtgctgc ctgggccagc aacagcgcgc gttcatagcc
    17101 ggccttgcgt gccaggattt cggccgtcgg gtcgaacagc tcgatgcgct cgtgcgtcgg
    17161 ggcgcaggca cgtgcggctt caaggaactg cagcgcatcc tccatatgcg gcgcttctgc
    17221 ccgcagcgcg gcctgacagg tgaatggagg aaattctgcc gcctggcgtt cgacgagcag
    17281 ggcgtcggcg tagccggcga aatcgtgacg gccgagcgcc tggaacagcg ggtggtgcgg
    17341 aaagtcggtc tgtatccata cctcgccggg ccattgcgcc cgccctgccc gtccggccac
    17401 ctgcatgagt tgggcgaaca ggtgctcggt ggcgcggaaa tcggcgctgt agagcgcagc
    17461 atcggcatat gcgatgccca ccagcgtcag cgcagggaaa tcgtggccct tgctgagcag
    17521 ttgcgtaccc acgaggatgt cggcctcacc ggcatggatc gctgtctgca ttgcctccca
    17581 cgctgtcggg ctggcggcgc tgtcacggtc gacgcgcagg atgcgcgcct ggggaaagcg
    17641 cgcgttgagc gtgtgttcga gccgctgggt accgaagccg cccgggtgaa gcggctgggc
    17701 acattgcgga caggtcgggt cgacctcgcg ctggtaacca cagtggtggc agcgcaacag
    17761 gcgtgactga cggtggagca ccagccgcgc actgcagcgc gggcagccgg ctgcccagcc
    17821 acattcgcga caatacagcg caggggcgta gccgcgccgg ttgatgaata acaggctttg
    17881 ctgtccccgc gccagattgt cccgcagcgc tgtctcaagc gccggagtga gcccttccac
    17941 cggtttttgc gcgcgggtgt caatgaggcg tatggcaggc agacgtgcgc cggcgatggc
    18001 gcgctgtgtc atgtcgatgc gctggtaacg tccgatgcgc gcgttgtgcc agctttcgag
    18061 cgaaggggtg gccgagccga gtaccaccgg acagccaagc cgctgtgcgc gtaccacggc
    18121 aatgtcgcgc gcggcgtagc gcaatccctc ctgctggttg aaggcggggt cgtgttcttc
    18181 atccagaatg atgagctgca gcctgggcat gggggtgaac agtgcaagcc gcgtgccgag
    18241 cacgatgccc gcctcgccgg tggcggctgc acgccaggca cgcagccgtt gtgtttcgcc
    18301 aagttcgctg tgcaacagcg ctaccggatc gcgaccaaaa cgggcatgga aacggcgggt
    18361 cagttgcggc gtgaggcgga tttccggtac cagcaccagc acctgccccc cctgctgcag
    18421 aatgcgctgc gtccattgca gatagacttc ggttttgccg ctgccggtca ccccatacag
    18481 caggaaaggc tggaagcgac cgaatgctgc tacgacggtg tccaccgcag cctgctgttg
    18541 cgcattgagt tcgggtgcag ggtgcagcac ctcgtccgtc gccgctgcgg cggtgcggat
    18601 cagccaaccc cgttgttgcc agacgcgcac gatggcgcgc tggctggatg acagtgtgcg
    18661 caggcgttcg gggctgagca ccggagtggc gagcaggagt tcgcgcagcg cccgctgcgc
    18721 gcgcgcgc
//
LOCUS       DAIANL010000145        17888 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1214137, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000145 DAIANL010000000
VERSION     DAIANL010000145.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 17888)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17888)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17888
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214137"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3220 a         5171 c         5968 g         3529 t
ORIGIN      
        1 tggtcaccta tctcgaaatc atcaccgcgc tgcatgaccg gttgcaacag caggatggcg
       61 catccgtggt cgtgcaggat gcggctgccg acgacccgat cagcgtctgg gcaacgcagt
      121 ggtatcacag cagcagcgat cattcgttga ctgccatgct cgacgcagcc atggaacgca
      181 cctattcggc caacccggat gtcactttct tcactggtgg cggcgtgcac catttcgtca
      241 atttcgacaa gcgcgaagac catatgatca tgaatctgtg gcaggcgaca agcgattcgg
      301 tgaacctggt gtatatccgg ctgatgcgcg atatcgtgca ctattacatc gcgcacatgc
      361 ccggcgtggc cggcagcatc ctgcaggatg tacgcaaccc ggagcgccgc acctatctgg
      421 agaaattcgc cgatcgcgag ggccaggaat tcatgttgcg cttctacagg aaataccgca
      481 atcgcagtcc gcagcagatc gatgcgatcc tgttcgatga agttcatccc agtgcgcgcc
      541 ggttggccgc gctgtaccgg tatctggcgc cggatggctc gattcagggc ttcgaactgt
      601 ttctgcgcag ccgcgcgctc aatccgctga gtgacgccag ggtggctgcc ctgtacaacg
      661 aatttgctcc ggggcgctgg tcgctcaatg atctgggcta catcatccag atccacccgc
      721 tcgaactgtg gctggcggcc tatctgcgca atcatcccgg cgcaacgttc ggccaggcgg
      781 tagccagcag ccatgaccag cgtattgaag tgtacaactg gctgttccgt gccggacgca
      841 aggatgcgca ggatcagcgc atccgcagcc tgctcgaggt agaggccttt gccatgatcc
      901 accaggactg gaaacgtctg ggttacccgt ttgacagcct ggtgccatct tatgccaccg
      961 ccctgggtgc atcggccgac cggccggccg cgctggctca cttgatgggc attctggtca
     1021 acgatggcgt cagtctgccc gaacgttcga ttgaccggct ggatttcgct gcaggcacgc
     1081 cctatgaaac cgattttgcc ctgcgcagta cgtctgggca gcggctgttg ccggtcgaac
     1141 tgacccgggt agtgcgccgg gcgatcgagg gggtggtaca gtccggaacc gcggtgcgca
     1201 tgaaagatgc tttccgtgct gccgatggca caccggttga ggtgggcggc aagaccggaa
     1261 ccggtgacaa ccggtttgat actttcgacg cgcaggggaa tgtgataacc tcaagggtcg
     1321 taaaccggac tgcaaccatg gtatttttca taggcgaccg tttttttggc acgatgaccg
     1381 cagtggtgcg tggcgaggca gctggcgatt accatttcac cagcgctctg gcagttcagc
     1441 ttattgctga catggggcca tggttgttgc agccgttcga tgcggccagt gcgggtccgc
     1501 tgcggcgcgg tgtaccgccg gtatcggccg aatagacagg gcggatttca atgcaactgc
     1561 tggcattcgg actcaatcat cagacggcgc cgctcaccat ccgcgagcgg gtcgcgtttc
     1621 atgtcgagca catggaatcg gcattgcatg atttcgtacg cagcggcacg gtgcgcgaag
     1681 ccgccatcct gtctacctgc aaccgcaccg aagtgtattg caacaccatc gaccacaccc
     1741 gggcgctgga ctggatggcg gattaccatc agctgccgcg cgaaagcatc gccccttacc
     1801 tgtacacgct gcccgaaacc gaagccgcgc ggcacgcgtt tcgcgtggcg tccggcctcg
     1861 actcgatggt gctcggcgaa acgcagattc tcgggcagat gcgcgaggcg gtgcggattg
     1921 ccgaacaggc gggaacgctg ggtgcaatgt tgcacaagct gttccagcag acatttgcgg
     1981 ttgccaagga tgtgcgcacc agcaccgaaa tcggagccag ttcggtgtcg ctggctgcgg
     2041 cggcggtgcg tctggcggaa cgtattttcc cgagcattgc cgaacagcag gtgctgttca
     2101 ttggcgcggg cgaaatgatc gaattgtgcg ctgcgcattt ttccggacgc cagcccaggc
     2161 ggatcatggt ggccaaccgt acgctgccac gtgcgcgcgc gcttgccgac cgttaccagg
     2221 gcgaggccat tgcgcttgcc gaactgcccg aacatctgca cgcgttcgac atcgttgtga
     2281 cctgtaccgc cagtccgttg ccgatcctgg gcaaagggct ggtcgagcgc gcgctcaagg
     2341 cacgacgtca ccggccgatc ttcatggtcg atctggcggt accacgcgac atcgaatccg
     2401 aagtcgccga actgtccgat gtgttcctgt ataccgtgga tgatctcggc gccgtggtgc
     2461 agcaggggct ggacaaccgc cagtcgcagg tcgggcaggc cgagcagatc atcgaggcgc
     2521 gtgtgcagga gttcgtgcac tggctcgata tgcgcgaagt ggtgccaaca atacgcagct
     2581 tgcgtgatca tgtcgaccgc gtgcgccgcc atgaagttga gcgggccatg aagttgcttg
     2641 cgcgcggcga tgcgccggag caggtactcg agcagctgtc gcgcagtctg accaacaagt
     2701 ttctccatca cccaacgcat gcgctgcatc atgccgacga ggccgagcga cgcgaactgg
     2761 tggcagtact ctcgcgcttg tattcgctgc atcccgattc atgaaaccat ccctgctcaa
     2821 caaactgggc cagctcgcgc tgagactgga agaactcgat cgcctgcttg cgtccgagca
     2881 ggcgacgcac gacatggagc gttatcgtca acttacgcgc gagcatgcag gtatcgcacc
     2941 cgtggtcgag ttgtatcggc gttaccagcg caacgagtcc gatctgaccg aggcctatgc
     3001 catgctcgac gatgccgaaa tgcgcgagct ggcagaacag gaattggccg atggccggct
     3061 gcgccgcgaa cagcttgaag ccgagctgca ggtggcgctg ttgccgcgcg atcccaatga
     3121 cgaacgcaac ctgttcctcg aaatccgtgc gggtaccggt ggcgatgaat cggcactctt
     3181 cgccggcgac ctgttgcgca tgtatgcgcg ctatgcagag cgcaagcgct ggaaaatgga
     3241 aatcgtttcg gccagtccgt cggaactggg tgggtacaag gaagtcatcg tcaagctgat
     3301 cggtcacggc gcctggtcgc ggctcaaatt cgaatctggc ggtcaccgtg tgcagcgcgt
     3361 accggaaacc gaggctcagg gacgcatcca tacctccgcc tgcaccgtgg ccgtcatgcc
     3421 ggaagccgat gaactggcgg aaatcgacct caatcctgcc gacctgcgca tcgatacatt
     3481 tcgcgccagc ggcgctggcg gtcagcacat caacaaaacc gattcagcgg tacgtattac
     3541 ccatctgccg accggcatcg tggttgaatg ccaggacgac cgttcgcagc acaagaacaa
     3601 ggcgcaggcc atggccgtgc tggcggcgcg tatcaaggac cggcagatgc gtgagcaggc
     3661 ggccagggaa gcggcgacgc gcaagtcact gatcggtagc ggcgaccgct ccgagcgcat
     3721 ccgcacctac aattttcccc agggccgcat gactgatcac cgcatcaacc ttacgctgta
     3781 caagctggac gccatcatgg atggtgacct ggatgaattg ctcgatgtgc tggcggcaga
     3841 gcatcaggcc gaactgctgg cggccctggg cgaatgaatt ggactcggcg ctgacgattg
     3901 gtgaactgtt gcaccgcgat gcggtgcgct tgcgagccgc actccaactt gatgtgcgta
     3961 catctgcaat ggaggcgcga ctgctgttgg cagcagcctg cggtgtggag ccggtgtggt
     4021 tgctggctca cgatcgtgat ctgccgcatt ccggacagat cgccatatat ctggcaacgc
     4081 ttgaacgcag gctgtcaggc gagccaatgg cctatattct tggcgaacgc gaatttttcg
     4141 gcctgatgct gcgcgtgaca ccggaggtgc tgattccgcg tccggatacc gaattgctgg
     4201 tggaactggc tctggcgcgg ctgccgcagc gtggcggatg ccgggtgatc gaccttggca
     4261 ccggcagcgg cgcggtggca ctggcgctgg cgcacacccg gcctgatgca gacgtgctcg
     4321 gcgtcgatcg cagcgaagcg gcgctggctg tggccgccga caatgcgcgt cgtctgggga
     4381 tgaacaatgt gacgctgctc tgcagtgact ggtggcagtc cgttccggcg ggtcggtttg
     4441 agctcgtggt gagcaaccca ccctatatcg ctgcaggcga ccgtcatctc ggtcagggcg
     4501 atgtgcgctt cgaaccgttg caggcactgg tgggcggccg tgacggactg gacgatctgc
     4561 gccgtattgt ccccgacgct tgcatgcgcc tgacgccggg aggctggctg ctgctggaac
     4621 atggctggga tcaggctgct gcggtacgca gcctgatggc tgcggccggg ctgaagcaga
     4681 tcgaatcctg tgccgatctg ggaggaaacg agcgagtcac actcggtcag gctggctgat
     4741 cgggaggcca cattccgcag cggtccaggg tacggtccgc tgcggagtgg gggatcagcg
     4801 acggtcgttg ccgctgacgg cgatgaggat gttgagcagg ttgacgaaaa tgttgtagac
     4861 gtcgaggtag atcgccagcg tggccatgac gtagttggtc tcgccgccgt gtatcacgcg
     4921 gctcacgtcg aacagcaggt agcctgagaa gatcaggatc gcgatggccg aaatcgccag
     4981 cgatgccgca ggtacgtgga agaacaggtt ggcgatcgag gcgaggatca gcatgaccag
     5041 accgaccatg aggaactggc ccaggaagct gaaatctcgc ttgctgaagg ttgcgatggc
     5101 cgacagcgtg aggaaaatgg ctccggtgcc gccagcagcc atgccgatga gctgtgcccc
     5161 gtttgtcagg tgcagcgcaa actgcaggat cgggccgagc atcacgccga gaaagaaggt
     5221 ggcgaccagc agcagcacga cgcccagact gttgttgcgg ttggcggcaa tacccatgaa
     5281 caggccgaac atggccgcca gcatgaggag cgacgacagc agcggatgct gtgccatgaa
     5341 cagcaggttc atgctggtgc cgatccatgc gccgattacg gtcgggatca tggtcaggcc
     5401 cagcatcatc cacgtgttgc gcagcacgcg atgcgattca cctgcgagcg aggtgggcga
     5461 agaatatacg gggaaatccg gttgcataga gtacctctgt gtaggaattg cgtttgtagc
     5521 gtagactata ggagcccggt aaaagttgca accttatatg gatgacggaa cagcgtaatt
     5581 caagtgtttt ctggtgcgcc gggaaaagga ggatgacaat gaacaccgca gtcagagggg
     5641 tggacgtgtg ggtcgcacgg ctggatgggg cgggtatccc ggtgctcaag cgtacagtgc
     5701 gcgaaatcgc ccgattgcag gccgacgccg agaataccga agttcgcgat gtggtggacg
     5761 tgctgagcca tgatccgctc atgacgctga tcctgctgcg ttacctgcag cagcacaaac
     5821 gcagctcgca gctcaacgaa gtggtgagga tcgaacaggt gctgatgatg ctggggctgc
     5881 ataccttttt tacccaggtc gcaccgcagg gcattgtcga ggagcatctc aaggggaaac
     5941 tcttcgcatt gaccgggctg ctgcacgctg tcaagcgtgc cacgcgcgca gcctggtacg
     6001 cgcgcgactg ggcggtgcgg ctcaaggatc tgcgttttga cgaaatccgc atggtggcat
     6061 tgatgcacaa ctttgccgag attctgatgt ggtgccacgc tccggaagaa atgacccgca
     6121 tccataccct gcagatgcag gatcggacgc ggcgcacacg cgaggtgcag cgcgaggtgc
     6181 tcggcttcag gctcgccgat ctgcagctgg agctggccag gcgctggcaa ctgccggagt
     6241 tgctgattga tttcatgcag cagaattaca gccaggaaaa acgcatgcgt accatcgtgc
     6301 tggcgatcaa tctcagccgt catgccgaaa acggctggaa tgacgccgcg ctgccggatg
     6361 attaccgtga cattggcgaa ttgctgcaca tgacggaaga agaagcaatg atcatggtgc
     6421 agggtccgca agtggccgtg ccgatgcact agccgcctgc tgccccgaca ggcgtgcgcg
     6481 ttcgaccctg tcaggcacgt tcgaacaatg ctaccgattc gacgtgcgcc gtctgcggga
     6541 acatgttggc gattccggcc gtacgcagtc ggtagccttt ttcatggacc agcaccgcgg
     6601 catcgcgcgc cagcgtggcc gggttgcacg agacgtagac gatgcgctgc ggcgcgtcct
     6661 tgccgagcgc ctgaaccacg gcgctggcgc cgtcgcgggg tgggtcgatg agcatcttgt
     6721 caaaatgtcc gagcgccgtc agcgaggctg cggtcgcttc gaacaggttg gcagtttcga
     6781 aacgggtgtt gtccaggcca ttgcgcgcag cattctgccg cgcacgtccg accagttcgg
     6841 cgctgccttc cacgccgacg acgctctgtg ccgatcgcgc aatgggtagg gtgaaatttc
     6901 ccaggccgca aaacaggtcg gcaatgcggt cactgcgctg tggttgcagc agttgcaggg
     6961 cgcgtctgat cagtaccgga ttgaccgcag tgttgatctg ggtgaactcg ctcgggcgaa
     7021 acggcatcac caggtcgaat tcgggcaacg tgtaggccaa ttctggtgcg tcggacggat
     7081 agaaggcggt gatgctgtcc ggccccttgg actgcagcca ccattgcacc gtatacctgt
     7141 cggcaaactc gcgcagcagc tgcaggtcgg cctctgtcag cggcgccatg tggcgcagta
     7201 ccagcgcgtc gaccgcttcg gtggccacga cttcgatctg tggcacctgt gcgcggatcg
     7261 tcatgcgtcc gaccagttcg cgcagcggct ggatcaactg cgagatgcgc ggtggcagcg
     7321 tttcgcacga gatcatgtcg gcgatgaagc tgttcctgcg ttcatgaaaa ccgaccagca
     7381 cgcctccctt tttttccacc agacgtactg acaggcgcgc gcgggtgcga tagccccacg
     7441 ccgggccgtg aatgggcggc agccagtagt cggcgcgtac cttgcccaga tgtgccaggt
     7501 tgtcttcgag aatacgctgt ttcgcagcca cctggccgac ggggtcgatg tgctgcaggc
     7561 tgcaaccacc acagacgccg aaattcgtgc agcgcggcgt tacccgcagc ggactggccc
     7621 ctaccacacg cacgaggttg gcattttcgt agctgtcttt tttgcggtag cttgagtatt
     7681 gcaccagctc gccggtgatt gcgccctcga tgaacagggt cttgccgtca ctgcggtgcg
     7741 cgatgccccg gccttcatgg tcgagtgatt cgatgcggac cgtttccggc acaacgggag
     7801 cggtagtcaa ggcagttcag cccagtgcgg cgacaatggc atcacgcacc gattccacgc
     7861 tgccgatgcc ggaaatgcgt gtgtatttcg gcgccgattg cgcatcctgt ctggcccagt
     7921 cagaataata gtgcaccagt ggctcggtct gggcgtggta gacgtcgagc cgtttcttga
     7981 cggtttcctc gcggtcgtcg tcgcgctgga tcagcggttc cccggtaacg tcgtcgattt
     8041 cggccacgcg cggcgggttg aattgcacgt gataggtgcg gcccgacgcc gggtgaaccc
     8101 ggcgacccga catgcgttgc acgatttcgc tgtcgggtac gtcgatttca atcacatggt
     8161 cgatcgacac accggccgat ttcatggctt cggcctgggg aatggtgcgc gggaatccat
     8221 cgaagagaaa accgttggca cagtccgcct gggcaatgcg atccttgacc agaccgatga
     8281 tgatttcatc cgaaaccagt tgtccggcat ccatgaccgc tttggcggcc agtcccagcg
     8341 cactgccggc ggcgatggca ttgcgcagca tgtcgccggt ggaaatttgc ggaatgtggt
     8401 agtgttccct gatgtaattg gcctgggtgc cttttccggc gccggggccg ccgagcagaa
     8461 tgatgcgcat ggtgggtcag ctttcgttgt ggttcgataa ttcgttcagg acagcaaact
     8521 ggcgccgtgc gatcgccagg tcttccgcag tatccacgcc gggcgcaacc ggagtctcgc
     8581 tgatgcccac gcggatgcgc cagccgtgcc acagggcgcg cagttgttcc agcgcttcga
     8641 tctgttcgag cggcgcgggg ggcaaggcgc agaagcggcg cagcgcgcca gcgcgatagg
     8701 cgtacaggcc gacgtggcgg aatatgggta gcggatcgtg ttgctgcagg gcggcttccc
     8761 agccatcgcg cggccacggg atcggagcgc ggctgaaata gagtgcgtgg ccagactggt
     8821 cgagcacgac cttgacctgg ttggggttgt tctggctgtc ctggtcgtgg atcgggtggc
     8881 acagcgtcgc catgtcggat tgcgggttgt gcgccagcag gtgggcaact tcgcgaatgg
     8941 ccgccgggtc gatgagtggt tcgtcgcctt gcacgttaac cacgatggtg tcgtcggtga
     9001 cccccagccg gtcggcggcc tcggccagcc ggtcggtgcc ggaggcgtga tggccggcgg
     9061 tgatcaggca gggggcatcg tgtgctgctg ctgcagccac aatgcgttcg tcgtcgcagg
     9121 cgatccatac cgatgcagcg ccgctgagtc tggcgcgatc ggccacacgc accaccatcg
     9181 gtttgccgtg aatgtcggcc agcggtttgg cgggcagtcg cgtcgatgcg tagcgcgcgg
     9241 ggatgatgac aatgaacggc accatcaccg ttcagcctgg atgcctgacg ccgtgccggc
     9301 ccgtgcctgc atcagacttc ctcgtcggca gcgagtgggc gtgcctcgcc ggcaagcatg
     9361 acgggaatgc cgtcgcggat cgggaaagcc agccggttgg tcttgcatac cagttccccc
     9421 gcattttttc ggtacacgag cgatcccttg cagattgggc agaccagaat atccagcagt
     9481 gtgctatcca tgatgttgtt ccagcttgag gagtacgaga tcgagcagac cggcgtccgg
     9541 tcgtgcactg accggccata cccacatttt ttcggtagcg aaatcacgac attttactgc
     9601 atctttggct gtcatgagga tggttccggg aaaattcagc tcttcggcgc gataagggtg
     9661 gtggtcaggg aaggggtggg cgataaactg caggccggtg gcacgtaact ggctgaagaa
     9721 acgttctgga tggccgatcc cggccacggc gtgcagcggc tgatcgtgca actgcgtgat
     9781 cgccaggcga cgttcgggga atttcaggtt atagagcgca tgcgtttcca gttgcatggt
     9841 gaactgctgc ggccagcgtg aagactgttg cgctggcggc atcgtgccat gcacgatgac
     9901 tgcatcgaca cggtcgaggc gtgccagtgg ctcgcgcaat ggcccggccg gcagcaggtg
     9961 accgttgccg aatccgcgct tgccgtcgat gagtgccagt tcgcagtcgc gagccagcgc
    10021 cagatgctgc aggccgtcgt cgcaaatcag tacgcgaacc tgcggatgcg cagcaagcag
    10081 ggcgcgaccg gattgcgccc ggtcggcacc gcaccatacc ggcagcccgg tggcttgcgc
    10141 cagcacgatg gcttcgtcac cgacgatggc cggatcgctg tccgcgttga ccgcctgggg
    10201 ggtatgcgca ctgccgccgt agccgcgaat gagtatgccg gcatgccagc cacaacggtc
    10261 gagttcggac gcgagccaga tggcaagcgg ggtttttccg gtgccaccga cactcacgtt
    10321 gccgatcacc accaccggta ccggcagccg gtgcgtgacc aacaggccgt agcgatagag
    10381 gaggcgacgt atggcgacgg ctgcgccgta cagccaggat aagggacgca gcaggaactg
    10441 ccagccgtcg ggatgttgcc actgccgtgt cagccatgcc tcgaggccgg aattcaccac
    10501 tcaaaaaccc gctcgaccca tgcctgcaga tcggcctgag ccagtcggcg ctcctgccag
    10561 aatgcgcgcc gtgccgagtc accggcaacg cgtgccgtga tgggctgggt gcgccagttt
    10621 tgccaggcag tgaacaggtc tccacgtgcg gcactgtctt cgagggtggc cggcagcgcc
    10681 tccgctgccg gatcgatgtg gatggctgtg ccaccggcca gtgcctgcca cagaggtttg
    10741 cgcgacggct gggcgaaatg tgcgccggtt accgaaacgg cgatggccgg cagccatttg
    10801 tcttcgtcga tccagacgat gctgccgtca gcaaacggcc ggcgatccca gtggctgatg
    10861 cgctgcgcgc cagcttgcgg cgcgcagcca ctgatgaata cgatgtcttc gggatggcgt
    10921 tcgatccagc gtcctgccag agccgtggca gcagcagcat cggcatcgtg cagccaccac
    10981 aggtgccgtt cgcgcccctg gttggccagc gagcggaagt tcgggtcgac ctgggcttcg
    11041 acgagcaagg taagcagatc tgcgccgttg ccgacgggtt ggtgtgcaaa gttcgcacga
    11101 tgcggacggg tggtaagcac gcgcgcttgc cggtcgagcc agtggcgcag gtggggccgc
    11161 ggacaggcat cgaccacgag cacgccgtac gggtcgagtc gctgcagcac ccggcgcagt
    11221 gagcgcgggt ggtcggccgg cgcaaagccc cagccggtct tgtcgcattc gtcgagcgga
    11281 gcgagcaggt cgggatattc ctcttcaaag gtcagcacca gccgcaggtc gagtcgcctg
    11341 gcgcggatgg cgcgcaacag ttcgactgcc tggtgtacgc tttcgcggct ggacccggcg
    11401 aggatccaca ccacttttcc ctgttcgccc atggggttga gccagccacg gcgtgcggcg
    11461 atgcgtgccc ggtttccacg tttgcgatcg gcgcggtcgg caagccagag tcccagccag
    11521 tggctgccga acggatcttt ccacgaaggg ccgggacaag ggtgcatggt ggtgtgcgcg
    11581 tagcgcgtgc tcagttggtt ttttgcgtgg cgaaggtgat gcgtgtcaga ccggcttcac
    11641 gcccggcctc catgacattg atgaccgact ggtgggtggc cttggcatcg gcatcgatga
    11701 cgatgacagt ttcgggcgat cccttggcag ccttcctgag caggtcagcc agattctgca
    11761 cattggagaa actgatcggg ctgttgtcaa tctggtagtt gccggcagca tcaacggcca
    11821 cggtgacctg ctgcggcttt tccttagtgg tgtcgccatt ggcctggggc aggttgatct
    11881 gcagttcgga gaacctggaa taggtcgtgg tgaccatgag gaagatcagc accacgagca
    11941 gcacgtcgat catcggaatc aggttgattt ccggttcgtc gggctctttt ctgcgctgga
    12001 agttcatgtg tgctttccgt cagttgcgtt cgccctgcac cgtctcgacc agcttgatgg
    12061 cttcctgctc catgtcgatc accagcgaat cgacgcgcga acggaaaaag cggtagaaga
    12121 tgaggctggg aatggcgacg atcaggccga aggcagtatt gtacagtgcc gtcgagatac
    12181 cgtgggcgag tactgccggg ttgctgccgg tgctggtttg tgagccgaag atttcgacca
    12241 tgccgatcac cgtgccaagc aggccgagca gtggcgagac cgaggcgatg gtgccgagtg
    12301 ccggcaggaa gcgttcgaga tcgtgcaccg cggcgcggcc agcttcctcg atggattcct
    12361 tcatgatgtc acgcgaattc ctggcattgc gcaggccagc ggaaaataca cgtccgagtg
    12421 gcgagctttg cgccagcagg ccgagcattt ccgggttggc gccctgttgc cgccaggcct
    12481 gcagcgtttt ctccagcagg tctcttggcg cgacctcact gcgacgcagc gagatgaagc
    12541 gttcgataat gatggcgacg gacagaaccg aagccagaat gagtgtccag atgggccatc
    12601 cggcagcttc gacgatcgat aacacgcttg gactccgatt gtgaaaacgg gcgttattgc
    12661 aacaaaacga gcgacacttt agcctgagat tgtgattagg gcaagcttgc cccggattgg
    12721 ttccttgcca ggcgttgcaa agccgcacct gtgcggttgt tcactgccag gcaccgagtg
    12781 agcgccaggc aaagccatct tgctgggtgg cgcaatccag ccagtccggg tggcaaccca
    12841 gcgcctgggt caacgcgttc agcgcgagct gcggcgtgtt gttctgctgc gacaggtgcg
    12901 cagccaccag gtgctgcagc cggctgcagt cgatgcggct gagcaggctg gcggcggcgc
    12961 tgttgtcaag atggccgaac tggccgccga tacgctgttt gagaaacggc ggatagcgac
    13021 tgttgcgcag cagttccgga tcgtggttac attcgagcac cagggcatcg cagccgtcga
    13081 gcatcagggt gatgtgcgcg gtgatgctgc cggtgtcggt gagcacaccg agacgatggc
    13141 gaccatcgct gaacacgaac tgtacgggtt cgcgggcgtc gtgcggcaca ggaaacggtt
    13201 gcacctgcag ttcgccgact gtaaagcttt cgtggctgtc gaggatgcgg acatccggca
    13261 gcggatcgtt cgtccgtaac gcttgctgta gcgtgccgtg cgtcatccat aatgccgcgc
    13321 cgctgtggcg ggcaaagcgc gcggctcccg acaggtggtc ggcgtgctcg tgggtgacga
    13381 tcacggcgtc gatctgcgct gcggacaggc ctttttgttc gagccgacgg caggcatcgc
    13441 gcaggccgaa accgcagtcg agcagaatgc gcgtcgaccc cgtttcgacc accagggcat
    13501 tgccctcgct gccgctgcca agactggcga accgcatcat gccgctcgtg tcctgttgcg
    13561 cgtcgtcaca tgacgctcac ttgagctggt cgtacagctt gccgaggatg cgtttggtga
    13621 cgtcggccgg agcagtcttg ccagtggcac tgaggatgtg aatgtcagtg ctggtggccg
    13681 ttgcattctc gtccagttcg acctgatact ggttgaagtt gtcgccggtg tgtttccaga
    13741 attcgagctt gttccagaat ccgccggact ggctgttgtc gacattggtt ctggcttcgg
    13801 tgacgaagta cacgcccttg gtgcggtcgc gatcgacgac gttgtacccg acgcggtcga
    13861 gcgccagtcc gaccctgcgc caggcgcggt cgaacggttc ctggtcggtc aggacctgtc
    13921 cgccgtcagc cgactggttc aggtgtgcca ggccgctggc ggccggtgtg gcagccacca
    13981 tggcctgcgc cttgggtttg ggtacgccga aacggcccat caggtcttcg agcaccagcg
    14041 cttctgcgcc ggggtcgggc ggaagccgct gccactgggt gtgcagtccg tcgatcgagt
    14101 tgtcatacac ctgggtgacg ctgcgttcgg agacatacac ttcggtctgg ccgggatgag
    14161 ctgcatcgcg ttcgatacgg gtacggaacg cattgcgctg gtcgctcgag tacagactgg
    14221 gtagtacctt gcccagcgtg ctgtggatga tgtcctgcgg cagcctggag gcgctttcct
    14281 tccagcccgt ctgcagcagg ccgagttgcg ggttgttttc gaccaggtcg taacccttgt
    14341 ctttccagaa ttgctccagc accggccaga ccttttctgc tggtgcatcg atcaccagcc
    14401 agcgttcgca gccatcgcgt tccagtctga cgccggcggg tgctgcgggc agcacctcgc
    14461 ttgcaccgga ttcggcggat gccggttgtg gtgtcgcttc agcctgcgtc gtgtcaggct
    14521 tggccgtgtt ctgctggctg gccgaccatg acgaccaggt ggcgttgccc ggcttggtgt
    14581 tgccttgcac ctggtactgg ctgtcggtgc tgggcgatgt caggtcgggc ggcacttcaa
    14641 gtttcgcgat ctgggtggtg ttctggtagt cgaccggcgt ggaacggaaa atgctctgga
    14701 tggtagagca accgctgagg ctggccagca ggccggagcc aaccagcgcc atgagcaggg
    14761 tggagcgttt cattcggaag catcctcggg aagagattgg gctgaagcgg atgggtgttg
    14821 cggtgcggcc tcggcgcggc gcatggccgc acgcacacgc tcgtgatgga tgggatcgag
    14881 tggggtcaat ggcagacgca ggccgtggcc gattctgccg gcttcggcca gcgcccattt
    14941 gaccggtacg ggattggcct cgacgaacag atcgacgtgc aggccgaaca ggctgtgatt
    15001 gatcgctcgc gcgagcggca catcggcgcg gaaggcagca gcgcagagct ggtgcatggc
    15061 acgcggggcg acgttggccg tgaccgagat cacaccgtgg ccgccgagca gcatgaaggc
    15121 aagcgcgctg gcatcgtcgc cggtgtacag ggcaaagtcg gtcggggcgt tgtggatgag
    15181 ttcggtcgcc cgctgcatgt tgccggtggc atccttgatg ccaacgatgc tgtcgatctg
    15241 cgccagacgc aacaccgtgt cgttggccag atcagccacg gtgcgtccgg gaacgttata
    15301 cagtatcagc ggcaggtcga cggcttcggc aatggtacgg aagtggcggt agaggccttc
    15361 ctgaccgggc ttgttgtagt agggaaccac cgacagggcg taatcggcgc cggccttgtg
    15421 tgcacagcgc gtgagttcga tggcttcgcg tgtggagttg gcgccggtgc cggcgatgac
    15481 cgggatcctg ccggcggctt gctcgactgt gacgcggatg agttcgcaat gctcggaaaa
    15541 atcgacggtg ggtgattctc cggtggtgcc gacgatcacg atgccatcgg aaccttgttc
    15601 aatgtgccag tcgaccagtt tgcgcaatgc gggaagatcg agttgtccat cgtcgaacat
    15661 tggggtgaca agcgccacca gactgcctgt gagcatggtt tccttacctg tatcgaaccg
    15721 cgcgtgcgct gcaatgaccg ggtcggcggc aggaaacgcc gtcccgcggt tggcaaaacg
    15781 tcagtgtacc tgattcgtcg ggccgagaaa atgcatagcc gtcatggacg gaaaattttc
    15841 ccgtttgcgc agcgttgcga caaaacctcg agatgtgtag cattgtgcgc gtcgtatcca
    15901 tggggaataa cggcttgagc gaagatatcg ttcgtcagca actgcagtcc ctgttgctca
    15961 aagtgcggga aaacgaggaa aagctgctca agtttggccg tctggaacag caattgatgg
    16021 cggcgcgcac gctggtgcaa ttgctctggg tcattttgca ggattttccc ggcagcttcc
    16081 tgatcgattc cgtccgtctg gtcctgcgcg accctgacgg cgaatgggcg cgtgcgctgg
    16141 gtgcggattc ggtgcgcact ttttccggta tcggtctgtc cttgcgtgac gatcttgcgc
    16201 agtgcgaacg catgtacgga cgcgagttgc atccggtgct cgcgccatac cgcaaggaac
    16261 ggcacggaga tctgttcgcc agcctggccg agacgccggg ttcggtggcg ctgctgccgc
    16321 tggtgcgcca gcaccggctg gtcggcagcc tgcatctggc cagcagcagc cgcacacgct
    16381 ttgccatcag cgatggcacc tattttctcg aacgcctggc agccttcatg gtgatgtgtc
    16441 tggagaacgc gctgtattac gaaaaactcg agcgttcgag ttggctggac gggttgaccc
    16501 aggtcaacaa ccggcgctat ttcgacgtgc gcatccgcga ggcggtcagc catgcgaggc
    16561 ggcgcggcgt gccgctctcg ctgttgatcc tcgatctgga ctatttcaag cgcgtcaatg
    16621 atcaatgggg ccatcaggcc ggcgattcgg tgctgcaaca ggccgcgcag gtcatgcgtt
    16681 cgctgttgcg tgccagtgac agcttcgccc gttacggcgg agaggagttc gtggcgctgc
    16741 tgccggacac cgactgtgag tcggcctccg agattggcgg gcgtattctg cgggccatcg
    16801 ccggatttgc ctttcatgtc ggtccaactg cgcctgtcga tatgaccgtg tccatcggcg
    16861 tggcacaact ggatggcgcc gcaccggcgc tcagcgacga ggacgccggc aaccgtctgg
    16921 ttgcgcaggc cgacgccgcg ctttatcgtg ccaaggaaca ggggcgcaac tgcgtggtgg
    16981 cggcagcgtg agcctgccgc aacagggtgt gctggcgctg gtgcatggcc agccgctgac
    17041 cgagttgccc gctgaactgt atatcccgcc cgatgcgctg gcggtgtacc tgcatgcctt
    17101 cgaaggcccg ctcgacctgc tctcatggct gatccgccgc aatcggctcg acgtcctcga
    17161 tatcccgatg gcgcggctga ctgaacagta catggaatat gtcgactgga tgcggcggca
    17221 gaaattcgag ctggcggccg attacctggt catggcggtc tggctgatcg agatcaagtc
    17281 gcgcatgctg ttgccgcggc cggcgctgca gtcggaagac gggcaggacg acgatccgcg
    17341 cgcgcgactg gtgcagcgct tgctcgacta tgaaatgatc cgtctggctg cagcacgcat
    17401 cgatgcactg ccgcggctcg agcgtgattt cctgccggcg cgcgcccagg cggaactgcc
    17461 agccgctccc ttgccgcagg tggttccgga ggatatccag caggcctggc tcgggttgct
    17521 gtcacgtacc ggcctgcgcg cacgtcaccg tgcggcacgg cgcgagttgt cggtgcgcga
    17581 gaccatggga cgcctgctgg gcggactgca gggggaattg ttcacagagc tgggcgaact
    17641 ggtcggtcag acggatggtt cacgtgctgc gcgggtgtcg cagtgggtgg tgcatctgct
    17701 ggcagcgctt gagctggcgc gcgaaggtca ggtcgagatt cagcagaatg aaccgtttgc
    17761 tccgatttat ctgcgtcttg tcggaggagc cgtcgatgtt gcatgacacg ccagccggac
    17821 tcaaggccgt gctcgaagcc ctgttgctga ccagtgccga accgcttact gcggcgcagt
    17881 tgcgcgat
//
LOCUS       DAIANL010000146         3882 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1621067, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000146 DAIANL010000000
VERSION     DAIANL010000146.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621067"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          747 a         1077 c         1215 g          843 t
ORIGIN      
        1 agcacgaaag cgcgatcggt ataggtggtg ccaacctgga agttggtcgt ggtttgcggc
       61 tgaatggtgt tgggttgcgt ggccagcgtg ctgcccttgg gatagaacac attcaggttg
      121 ggggcctgga agccttcagc cagttgcgca taggccgacc agtttttctg gatcatgtag
      181 tgcgcatcca gagagggcag ccagtcggtc caggtttccg aggtgctgag gccattgccg
      241 gccaccgcgg cgttaccctg gttcaccggc gcgttgagat tgcgggtgaa cgactcatac
      301 ttcacacccg gggtgatggt cagctgcggg gttggtttcc aggcgtagtc gatgtaggcc
      361 tgggtggtgt ccagcgtgtc tgtcatcagg cggtcgaccg cgctgagctg gccgttgtag
      421 ctgttgatgt agttcaggcc gccggtggtc atgtccattt cgtactgcca acgggtgttg
      481 tcctgatggt cgtaccacaa accggtgttg atcgttcccg gaccgagatc gtgtgacaga
      541 cgcagcaggt cgccgaccga acggtaatcc atggtcattt tctggccggc cacgtcgttc
      601 ggattggtgg tgccgttgat gttggtggtg ccgatgtcac ccggaacagt tgcatagccg
      661 cccggcccat aataacccgg gtcgatacca ttgaaaccgt tgtgttcata ggcataggta
      721 tacagcttgt tgtcgatctg ccagccgtcc actctggact ggataccaaa gtactcgaaa
      781 tcggtgtgga tttcgtcata gttgtatccc tggaacagcg ggctgttcgg atcttcgctc
      841 aacccgaaat tgttgccgta cttggccaga ttgactgcgg acgcaccata cggcacgttc
      901 tgccggatat tgttctgcat cgcgacgaag gacagcacgg tgttttcgcc aacgggcttg
      961 atcagcttgg cgaaaatgtt ggtgcggttc tggttggcgt tctgcagata gccgttggtg
     1021 tgatagtcct tgtagctgat gtaggcgcgg gcatccccgt attgcggcag cacgccggtg
     1081 tcataggaca ggccggccag gcgcgaaccc cagctgccca gtgtcaggaa cgggctgaaa
     1141 ttgctggtca tcgacggatc gcgcgactgg ttgtatatgg tgccgccgaa cgtcgcgtta
     1201 ccgatggtgc tggcatcgcc cggaccgcgg tcgacactca cgctgcccag atcctgtgcc
     1261 atgaagtaac tggtggagtg gtgggtgaag tcattggaat caccccaggg aatgccatca
     1321 aactcgacgt tgtactgacc gtcctggaaa ccgcggatgt acgggccgcc gccttgcgtt
     1381 tccatgccgc ccgggccatt ggggtcaatg ctccattcac tgggcgtcat ggcgatgatg
     1441 tcggggtagt tcgcaccagg ggccatgttt tcctggatat agtgctggtt caccgtggag
     1501 accggctcgg tggcggtcag cgaaccctgc gagggggcct gataggctgc cgattgcgga
     1561 tcggtggtca ccgctgtctg atcattgctg ctggtgctgg cgtttaccgt gccgaggtcg
     1621 acggaatcgg ccagcgcgag gtggctaccc atgaccagca ggctggcggc gaccagtcgt
     1681 gaaatggttt taagtctgaa cgcttgcggc gtgctcattg tgtgctccca ggtggattgg
     1741 tatgaataac gaataaccac ccaggaatat acggagccaa acttagcata cagtgaacgg
     1801 attgtgacct tttggttaca tccggaatca tgagtctcaa atccaggaaa tgtgcgtatt
     1861 atcaatatgc tgcataaaac ccggcacagc tggcacagac ggtgttttgg ttgcaatgtt
     1921 gttgtgatgc aacactttgg cgtgaattga tgtgaaacag gtacgccggt cagcgcagtc
     1981 ggtcggcggc agagtctgtt atcatgtgcg catgacacga cagatatttc tcgatactga
     2041 aaccaccggc cttgaacccc ggctgggtca ccgcatcatt gagatcgccg cggtggaaat
     2101 ggtcaaccgc cggctcacag ggcgttattt tcatcgctat ctgaatccgg aacgcgaaat
     2161 tgacgccggt gccgaggcgg tgcatggatt gagcagtgca tttctgcagg acaagcccaa
     2221 atttgccgac gtggtggacg agttccttca gttcatcagc ctggacgaac tggtgatcca
     2281 caatgcggca ttcgatatcg gtttcctgcg tcatgaactg gggttgctcg accgcgccgg
     2341 gcaactggaa cagaacccgg tactcgatac gctgcgaatg gcgcgtgagc tgcacccggg
     2401 ccaaaagaac aacctggatg cgttgtgccg gcgttatgaa gtggacaatg ccagccgcac
     2461 gctgcacggc gccctgctgg acgcagaact gctggcagca gtctatctgg gcatgacacg
     2521 agggcaggac agcttgatga tcggcatgga aacacccggt ggagaggccg gcgagatgca
     2581 ggagccgtta ctggtgcagc cacgcatcct gccggcaagc gcagaggaac tgcaggcgca
     2641 ccacagcgtc gtgcgtgcaa tcggcaagtc cagtcgcggc gcctgttttc tgtggtaggt
     2701 cagccggctt cgaagccggc ggcaacgacg gcctgtcgca tctcggccac gccgcagcgg
     2761 gccgggtcat aatcgatctc ggcgcgcccg ttgtccagcg acacttcgac ccggcttacg
     2821 ccaggcaggg cggagagcac acgggtcacg ctgttgacgc aaccgccgca ggtcatgccg
     2881 gataccggca atgtcaagtg ttccatcgat catcctctcg ttctgtcgcc cagcagggcg
     2941 gccactgtat ccagtccgcc gaaacggatc gagtgtctgg cctgttgacg caccacaggt
     3001 ttggcatgaa aggcgatcga aacgccagcg gcattcatca tgaccaggtc gttggcgcca
     3061 tcgccgatcg ctacggtctg gctggcatcg agatgcagtt tcccggcggt ctgcaagagc
     3121 cattccgctt tgccctgcgc gtccagaatg ttgcccagta cgcggccggt gagccgtcca
     3181 tgcacgattt ccagctggtt ggctgcagca tgatcgagtc cgaggcgctg ttgcagccgt
     3241 tcagtaaaaa aggtgaaccc gcccgacacc agcagcgtgc ggatgccatt ggcgcgcgca
     3301 tgcgccagca tggcctctgc gcctgacgtc aggcgcacgc gttcatcata cacgcgttgc
     3361 agcagcgcag catccagccc ctcaagcaac gcgacgcggc gcgtcaggct ttcgccaaac
     3421 tcgatttcac cgcgcatcgc cgactgggtg atggcagaaa cttcggtctt gaggccgagc
     3481 atgtcggcaa tttcatcgat gcattcgacg gcgatcagtg tggagtccat atccatggcc
     3541 agcaggccga aatccgtcgg gcgacgcgcg tcaggcaccc aggcgcagtc aagctgcgca
     3601 ttttcacacc attgcgtcac ccagtcggca tcgggtacgg cgttggcagg caggcgccac
     3661 aggcccggtg atactgctgg cgggaacgct tcgccggtat gttcccgcca cgccgcaagt
     3721 gctgctggcg tcaggttttc tgcttgaata atcaggttat tatccatcat ttcaaccaag
     3781 ttccttgaat agttctcgcg tgcgcgccgc gcgttcgcgc cacccgggtg tggagatctg
     3841 gatacgcaac cgatccggac cagccagctt gtaatgtgat tt
//
LOCUS       DAIANL010000147         7694 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2836570, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000147 DAIANL010000000
VERSION     DAIANL010000147.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2836570"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1546 a         2520 c         2222 g         1406 t
ORIGIN      
        1 gtctggcgga cagttgaccg ctccgtgtgt gttccgctcg gccggcggcg ccgcccacca
       61 gctcggcgcc cagcattcgg cgcgcatgga aaaggtattc atgggtatcg ccggtctgcg
      121 cgtggtcacg ccgtccaacc cgaaacaggc ctatggcctg ctcaagtctg ccatacgctg
      181 cgacgacccg gtgttcatca acgagcacga actcatgtac aacatgaagg gcgaagtgcc
      241 ggacagcgaa tacctgcacc cgctcgaggg ttcggatgtg gcccgcagcg gcacggatgt
      301 ctcgctgttc ggctacaaca tctcggtgca ctggtgcctg aaggccgccg aaatcctgca
      361 ggccagacac gggatttccg ctgaggtgat cgatctgtac gccctgtcgc cgcttgaccg
      421 cgaaggcatc cgcaaatcgg tcagcaagac gcaccgtgct gtggttgcag aagaagacga
      481 ggctccggtc ggcgtcggcg ccgaggtcat ggccatcatc aacgaggaat gtttctttga
      541 actcgacgcc gcaccggtgc gtgtgcactc ggccctggtc ccacaaccct ataaccacac
      601 gctggaaaaa gcagccatcc ccgaccacga agatatcgtg cgtgcggtgc tgaaactgtt
      661 cggcaaagcc tgacgagggt tcgcacatga ccgcttcaca ccattacatc atcaccatgc
      721 cccaactgtc ggacaccatg accgaaggcg tggtggtcag ctgggaaaaa cgcatcggtg
      781 accgcgttga acgcggcgac atcgtcgcca ccgtggaaac cgacaaggcc atcatggatg
      841 tcgaggtgtt caagcctggc tatctggtcg gtccgctggc cgaagtcggc gccacggtag
      901 ccgtgtccgc cgccatgggc tacatcagcg attcgctggg agaagcgagc attgccagcg
      961 gcgaagtggt caccgacgtt gccccgaccg aaatgattcc gcaccattcg ggcatcgcaa
     1021 tcgccatgcc gcagttgtcc gacaccatga ccgaaggtgt ggtggtcacc tgggaaaagc
     1081 gcatcggcga cgcggtggcg cgcggcgaca tcgtcgctac ggtcgagacc gacaaagcca
     1141 tcatggacgt cgaggtgttc caggaaggca ttctttccgg ccccatcgca ggcgtcggca
     1201 gcaccgtaca ggttggccat ccgatggcat tcatcgtggc cgccggtgaa acggtcaatg
     1261 acaccgaagt gcaactgtcg gcagcgcacc aggttgtcga aacgcatcac cagggcgatg
     1321 cgccagctgt cgtgccagca cgcccggcca tggcaactgc ggccgccgta gccagccccg
     1381 gcgcggcacc agccgcacgg cccgtcggac agtatgccac accgctggcg cgcaagctgg
     1441 caggccagac cggcgtcgac ctcaacaagc tgcacggcag cggacccaac caggtcattg
     1501 tggcgcatga cgtgcagcaa gcccggccaa gcatgaaaga agtggcgcat tcactgccac
     1561 aggtggaagt gccgggcaac ggccgtgcca tgacgtcgat ggaaaaagcc gtgtcgcact
     1621 ccatgaccgc cgcgctcacc atgccgacct tccatgtcac ggtcaatatc cgcccggaca
     1681 aactgattgc cgcggccaaa gcgcgcaagg tatcggttac ggttgcgatt gccaaggcat
     1741 gttcacaggc catgctgcag cagccgatgc tcaatttctg ctatcagccg gtcgacaaga
     1801 tcgtcgagcg cagcgaacac aatttcggca ttgccgtcgc tgccgatggc ggcgggctgg
     1861 tcgtgccgat cctgaatcgc atcgaagaca aatcccttga acaacttcag gcggagtggt
     1921 ccgaactgat gccgcgtgca cgggcgcgca agcttgcgcc ggcggaatac gccaacccga
     1981 ccttcaccat ttccaacatg ggtatgtttg gcgtctccca cttcaccgcc attccgaccc
     2041 cgggcatcgc cgcgatcatg gccatcgctg ccaccggccc cgaaggcatg ccggtatgca
     2101 tcaccgccga ccacagggtt gtcaacggcg cgcatgttgc acagtggctg accagcctca
     2161 aacagatcat cgaacagcct gaaagctggt tacaggccga agcgcccagc ccgattccgc
     2221 cgggcgactg ggatttcccg gtgatcgtac tgggcggcgg accaggcgga gaagattgcg
     2281 cgcgcgatct ggtcgaacac ggtgtcaagg tcgccatgat caacaatgcc ccgttacccg
     2341 gcggcgaatg cctgtggcga ggctgcattc catccaaggc atggcgtcac agcgccgacc
     2401 tgatccgcga ccgcgcacac gatgccgcca agggtgtgct cggcactgcc acccccatgc
     2461 tggactgggg ggtgctcgaa cagcaccgca agtcgatcct gcagacccgg ggcgagatgg
     2521 ccctcaagac cgacaagggt gtcaagatcg acgtacgcga gggattcgcc tccttcgtcg
     2581 atgcgcacat gatccaggta cagcctccgc aaggcgaggc ctacacgctc agcttcggtg
     2641 cagccgtgat cgccaccggc gccccgccgt tcgtgccgcc gatccccggt gcccgtgaag
     2701 gcctggccac tggcggcgtc atcacttcgg acaccatctg gaacctgccc gcaccaccgg
     2761 ccagactggc catcatcggc ggcggggtga tcggcatcga aatggcacag attttccatg
     2821 atttcggcag cgaaatcctg gtgcttgagg ccaaggaccg gattctggcc gagctggaaa
     2881 gcgatgtggc aaagcaactg acagacctgc tgcagcagga attcaccgtg gccaccggcg
     2941 tgaagattga tgccatttcc ggcacggcca gcgccatgaa tgtcagctgg accggtgcag
     3001 acggacagca gcaacaattc ggctgcgact acgtactgat ggccaccggc aagcggcctg
     3061 acaccagccg gctcaacctc gcggccgtgg gcgtggaact cgatggagct gccatccgcg
     3121 tcgatacgcg cggccgcacc agcacgccgc acatctttgc ggttggcgac gtcgtgggcg
     3181 gctacatgct ggcccacacc gcagcgacgc aagggcgcgt tgcagcatcg aacctgctgg
     3241 gcgaagctgt gaactacgaa caggacctcg actgcggcgt gaccttcagc cgtccggaag
     3301 ccggattcgt cggactgtcg ctggctcagg ctcaagccaa aggcatcgat gcggtggaag
     3361 ccaagatgcc gatcagcatc gatgccaagg ccatgatcag tggcgaaacg catggtctga
     3421 tcaagctggt ggcggacaaa aacagccatg tcatcatcgg cgtgcatttc cttgccgacc
     3481 ataccgacac cttgatcggc gaagcggtca tgatggtctc ggcacgactg acgctggaac
     3541 aggtggcgcg tgccatccac ccgcacccga cccagaccga gctgttcggc gaactggccc
     3601 ggcgcctact ctcgcgcctg cgccgcacgg ccagaaaatg acctgcaccg ctcggccgca
     3661 acacgagcaa cgcagcggac gggccgctgc gcactcggcc agcgcacgct agctcatgct
     3721 cctgcccgcc gccctgattt tcggagtact cgtgctggcc gggatgatgc tggcgctgca
     3781 gtcgcgcacg caacgcaggc tggacgaact gccatcgcag atcgatgccg ccatcgagac
     3841 gcgccaccgg gccatgctgg ccgacctgca ccaggggctg accgcccagt ccgaccgcat
     3901 gcagacagca ctgctcgacc attcggaacg gctgcgcagt ctgatgacca ccgaactggg
     3961 ccaactgcgc gaggccctgc acacgctgca atccgcccag gccgcagccc tggccggtaa
     4021 ccgcgaaacc ctgcagcaat ccctgcacga cctgtcgcaa tcgcaagccg gagaattggc
     4081 gcgcagccgg gaaaccttgc agttccagct ggccgaatca gctgtagcgc tcaaggctgc
     4141 gcaatccgat tttctcggca agacgttgca gtcgctggcc gatcaggggc gggccgacca
     4201 actgcttctg cagacaacgc tgcagcaggt cggcgcccag ttgacgcagg tcgtcgacac
     4261 cctgaacaag accgtcgaga gccgcctggc tgacatcagc ggcaaagtgg ccgaacgact
     4321 cgacgatggg ttcaagaaaa ccaacgagac atttgccagc gtgatggccc gcctcgccac
     4381 cattgacgaa gcacagaaaa agatcgatgg cctgaccagc aacgtggtgt cgctgcaaac
     4441 gctgctgggc gacaaacgct cgcgtggcgc cttcggcgaa gtgcagctgg aaagtctggt
     4501 acgcaatatt ctgccacctg acgcctgcgc cttccaggtg acgctgtcca acggcacgcg
     4561 tgccgactgt ctgctgaaac tgccatcgcc aaccggtaac gttgccatcg acgccaagtt
     4621 tccgctggaa aactaccacc gcatgatagc cagcgacctg cccgaggccg accgcgtggc
     4681 agccacgcgc accttcaagg ccgacatccg caagcacatc gatgacatcg cctccaagta
     4741 catcatcgag ggcgaaacct ccgaaggggc ggtgatgttt gtcccggccg aggcggtgtt
     4801 cgccgaaatt cacggcaacc atgccgaact caacgaatac gcccagcaac gtcgcgtctg
     4861 gatcgtctcg cccactacgc tgatggccgt gctcaacact gctcgcgcag tgatcaaaga
     4921 cgttgagacg cgccggcagg tgcatatcat ccaggacgaa ctggcacgac tgggcaagga
     4981 attcggccgt ttcgaccagc gcatgaaaaa actggccgac catattcgcc aggcccacga
     5041 agacgcgagc gaagtgcagg tgacgagtca gaaaatcagc cgtcgcttca gccagatcga
     5101 agccgtcgat ctggaacacc tgcgtcgccc ccctcctgcc gtgttacccg gagaagacaa
     5161 cgaaccatga atccggacga gatcgacctg ctgcgcgccc gcctgcttgg cgagactgcg
     5221 cgcgccgact gggctgaact gcgcaccttt tttgcgcgcg gaaaactgat ccgggtgcat
     5281 gcagaactgg atctgatcga ggtcgccgtg cagatcgcca ccgaccagag cagcgacatc
     5341 gaaatcctca tgcaatccgg caggcttggc ccgctcgacg atgccacggc cgtcgactgg
     5401 cagcagcgtc atccgccgct ctgggcggtt gtgctcgcgc cctgggtgct ggtacaggaa
     5461 cgtgcccatt gaaacttccg cgccaatgag cgtgcaaccg gccccaaacc ggcacgtgct
     5521 gagtctgctg ctggccggac tggccacact cgggccgttt tcgatcgata cctacctgcc
     5581 ctcgttcacc agtatcgaaa ccagcctgca tgccagtccg atgcagttgc aacagacact
     5641 gagcgtctac ctgctgtgtt ttgccttcat gatgctgttt cacggttcgc tgtctgacgc
     5701 ctatggccgg cgtccggtga tcctggcgtc gctgctcgga ttcatcgcca cctcgcttgc
     5761 ctcggccttt gccaccgata tcagcacact catcctgctg cgtggcctgc aaggtctgtt
     5821 tgccggcgcc ggcatgatcg taggtcgcgc catcgtacgc gatctacatg acggcccgga
     5881 ggcgcagcaa ttgatgtcgc gcatcaccat gatgttcggc ctgtcaccgg ccgttgcccc
     5941 gatcatcgga ggctggctgc acgacgcctt tggctggcat tcggtattcg tgttcatggc
     6001 actgctcgga ctgatgctgt tcgtgctgtg ctggcgtttt ctcgacgaaa cgcacccgct
     6061 gcaccaccgc aaccacttct cgcttcgtcc attgctgcgc aattaccacg aggtcgccag
     6121 ccatccgcat ttcctcttgc tggccctggc cgtcgcaatg cactttgccg gcttcttcct
     6181 ctatatcgct tcggcaccgg tattcatccc cgacacgctg cacctgcgca gcgatcagtt
     6241 cgccggacta ttcattccgg ccatctccgg cgtaattttc ggtgcattcc tctccggacg
     6301 tctggctggc aaactgccgc cacaggcaac ggttcggctt gcctatggct tgctgtttgc
     6361 cgccgccctg atcaaccttg tctacaacgc cttcctcact ccgacctggc ccctggccgt
     6421 gctgccgctt acgctctacg ccaccggaat gtcgttgtcg atgcccagca tcactttgct
     6481 tgcgctcgat ctgtttcctc atcatcgcgg actcgtctca tcattgcagg gcttcgtgca
     6541 aacactgctc aacgccgtag tcgccggagc catctcgccg ctgctttcca cctttccggt
     6601 cgcgctggca ctgggaatgc tcggattttt gctggccgga cgcctgatgt ggcaaggata
     6661 tctgaagcgg gccagctgat tgatacgcac aacaccatga cttgtggtgc cgattttcag
     6721 caaaccgaca ggatttggcg cttataatca ttccggtgta ctactgactg gatagttgca
     6781 tgcagcaaac agacgtcaaa aggctgttca tcgtgctcgg catgcatcgt agcggtacca
     6841 gtgcgcttac ccgtgcactc aaggtgttcg atatcgaact cggcaataac catatcccga
     6901 ccgagcccgg tgtcaacgcc aaaggcttct gggaagaccg cgacatccac acactcaatg
     6961 tagagatgct gcgcgccctg gcgatccgct gggaccacac cactccaata cgcccctcgg
     7021 acatccagta tctgtgcctc aaaccctttt tcaagcgtgc gcaggcactg cttgctgcgc
     7081 gcatgggtcg ctacgaacgt ttcggcatca aggatccacg tctgtcgagg ttattgccgt
     7141 tctggcagca cgtgtttgtg tcgctcgaca tacagccgca ctacctgatt gccttgcgcc
     7201 accctgcaag cgtggcgcaa tcgctggcgg tgcgcaatca cttccgcaag gaaaaaagtc
     7261 tggctctgtg gctcgaatac accatggcag ccctgcaaca ctctcgcgac acttcacgcc
     7321 ttgtcgtcga ttacgaccgc ctgatgcaca atccggtcag cgaactgcgc cgcatcggcc
     7381 agcattggaa cctgtgtctg aacgatgccg aactgcatca ctacagcaag aatttcctcg
     7441 accagtcgtt acgtcaccat cagggtagcg acccgctgcg taccttcatg gagatcggac
     7501 cgctgtacaa aattttttct gtgctgcaaa aggcggccgt cgatcaggtc gatctcgatc
     7561 gtgcggactg ctgtgcccag attgaccaat ggtaccgtca gatcaggccc agccacacct
     7621 cgcgtgcccg gcacacgctc aaacgctgga tccgcgcggt tgcgatcaac cgtagcgtgg
     7681 ctgggaaact ggct
//
LOCUS       DAIANL010000148        15645 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2435105, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000148 DAIANL010000000
VERSION     DAIANL010000148.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 15645)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15645)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15645
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2435105"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2892 a         4422 c         5002 g         3329 t
ORIGIN      
        1 ggtggctttg aacaagccgt atacattcgt ggtgatggcg atcctgattt tcattctcgg
       61 gccgcttgcc tacatgaaca tgcgtacgga tatttttccg gacatcaaca ttccggtgat
      121 cagcgtgatc tggacctata acggcatgcc accgcaggac gtggccgacc ggatcaccac
      181 ctattacgaa cgtaacctgt cgagcacggt gaacgacatc gagcacatcg agtcgcagtc
      241 ggtggccggg gtgtcggtgg tgaagatctt cttccacccc aacgtcaaca tcaatggtgc
      301 gatgagccag gtgacggcca tttcgcagac ggtgctcaag cagatgccgc ccggcatcac
      361 gccgccgcag attctggtgt acaacgcatc cagcgtgccg gtgatttcga tggcgctgtc
      421 cagtcaccgc ctgctcgaca acgagctgtt cgacctgtcc aacaacttca tccgtccgca
      481 gctggcacag gtggaaggcg ctgccgtgac ttcgccttat ggcggcaagg tgcggcagat
      541 ccaggtcgac ctcgacccca aggcactgcg ttccaagggg ttgacgccgg acgacgtcag
      601 caaggcgctg gcggtacaga acctgcagct gccatccggt accgaaaaga tcggcctgtt
      661 cgaatacaac gtgaccctca acgccagtcc gaaagtggtt gaagaactca acgacatgcc
      721 gatcaagacc gtcaacggcg cgacgatcta cctgcgcgac gtgggctatg tgcgcgacgg
      781 tttcgcgccg cagaccaaca tcgtgcggat cgacggccgc cactcggtgc tgaccaccat
      841 ccagaaaaac ggcaaggcgt cgacgctcga tatcatccag cagatcaagg acctgatgcc
      901 gatcatcatt gccggtgcgc ccaaggggct gcacgtgacg ctggtgggcg atacctccac
      961 tttcgtcaag gcgtctgtca acggcgtgat ccgcgaaggc gtgactgctg cgatcctgac
     1021 cggtctgatg atcctgatgt tcctcggcag ctggcgctca accgtgatca tcatgctatc
     1081 gattccgctg gccatcctgt ctgcgatcat tctgctgctg gtggccggtc agaccatcaa
     1141 cctgatgacg ctgggcggtc ttgcactggc ggtgggtatc ctggtggacg atgcgacggt
     1201 gaccatcgaa aacatcaact ggcacctcga gcacggcaag gacgtgcgta ccgcgattct
     1261 cgacggtgct gcgcagatcg tggtgccggc tttcgtgtcg ctgctgtcga tctgtatcgt
     1321 gttcgtgccg atgttcttcc tcggcggcgt gtccggatac ctgttcgtgc cgctggccga
     1381 agccgtggtg ttctccatga tgtcttcgtt cgtgctgtcg cgtaccctgg ttccgacggt
     1441 ggccaactac ttgctcaagc cgcatgtgca tttttccgag catgaatcat caagcggaca
     1501 cggggcgcat gccgaaggcg aggttgtctt cccgccgacg aaaaacccgt tcgtgctgtt
     1561 ccagcgcaag ttcgagcagg tgttcagcgt gatacgcgaa aattatcgca acctgctgct
     1621 gatggcgctg tcgcaccggc cgaagttcat cattggcttc ctcggcttcg tgctggcgtc
     1681 tttcctgctg gtgccctggc ttggacgcaa tttcttcccg gccacggatg ccggcctgat
     1741 caagctgcac gtgcgcgcca agaccggcat gcgtattgaa gagtcggcgc gactggtcga
     1801 cctgatcgaa cgcgatatcc gcagcaacat tccggccgac gaagtttcga ccatcatcga
     1861 caatatcggt ctgccggtca gcggtatcaa cctgagttac agcagttcgt tccccatcgg
     1921 aacggcggat gcggacatca tggtcaccct caaggaagac catcatccga ccgaaggcta
     1981 tgtgcagatg cttcggcgta aactggcgaa cgacttcccc ggcgtgacct ttgccttcct
     2041 tcctgccgac atcgtgaccc aggtgctgaa ttttggcttg cctgcgccgc ttgacatcca
     2101 gatcttcggt ttcaagaacg atgagaatcg ggcttatgcc aacgagctga tgacaaagct
     2161 gcgcaaggta tccggtgtgg ctgacctgcg tatcgcgcag cagttcgacg aacccgagtt
     2221 gcatgcggtg gtcgatcgtg cccgcgccca gcagcttggc ctgtcggaac gcgacattgc
     2281 caacagcatg ctgatcagtc tgtccggcag cttccagacg gcgccgaatt tctgggtgaa
     2341 tccgaccaac gacgttgact atccattggt gatccagaca ccgcagtacc gtatcgatac
     2401 cctcagcgat ctgctcaatg tgccgattcc gaccggcaag tcgagcaatc cgaagctgct
     2461 ggggggcggg cgctctgctg tgccgcagat cctgggcggg ctggtggaca tccagcgcaa
     2521 gcagcagacg gcgatcgtgt cgcactacga cgtgcaaccg gtgatcgaca tcttcgccac
     2581 gccggaaaac cgcgatctgg gcggcatttc agcggatatc cagaaaattc ttgccgacac
     2641 ggccaaggat gtgcccaaag gctcgactgt ggtgctgcgc ggccaggtgg aatcgatgaa
     2701 ctcggcatat catggcctga tcttcggtct ggtggcggca atcgtgttca tttacctgct
     2761 gatcgtggtc aacttccagt cgtggctcga tccgttcgtg atcatcaccg cgctgcccgc
     2821 tgccctcgcc ggtatggtgt ggatgctgtt cgtcacgcac accacgctgt ccgtgccggc
     2881 actgatcggt gccatcatgt gtatgggtgt ggcgaccgcc aacagtattc tggtcatcag
     2941 ttttgcgcgt gagcgtctgg ccgagggtga cagcgggctg gctgccgcgg tggaagccgg
     3001 ttatatccgc ttccgtccgg tgctcatgac tgcgcttgca atgatcatcg gtatggcgcc
     3061 gatggccctc ggcctgggcg acggcggcga gcagaacgcc ccgctcggtc gcgcggtgat
     3121 cggtggcctg gcattcgcta ccgtggcgac gctgctgttc gtgcctgtgg tgttcagtct
     3181 ggtgcaccgc gacaaggcag agccgcacaa ggccgatccg tcccttcaat tgtctgtatc
     3241 cggagcttga atcatgtcga atgaatatcc tcacgttcag gtatcgcgcc gtgtgcgcac
     3301 gatgctgatc gtcctgctgg cagccgcggg cctgattgcc acggagggca tcctgagccg
     3361 gctgcatgcc gacaagatgc tcgttgcggc cgccgagctg cgcgaaatca cgccggtcaa
     3421 aatgatcatg cccgggcatg gtgcggccaa ggaaaacctg cggctgccgt gtaccgtgac
     3481 gccatactac gaagcggaca tttattcgcg cgtcaatggc tatctcaaga cctggtacac
     3541 ggatatcggc gcacgcgtca aggccggcca gttgcttgcg gtggttgaaa ctccggatct
     3601 tgacgagcag atccagcgtg ccgagtccaa tcttgccacg caaaaggcca ggttgcagct
     3661 cgccgtcgtc acggctcagc gctgggaaaa actgctggca accgattccg tgtcgcgtca
     3721 ggaaacggaa gagaaagaag ccgacctgaa agcgcagcaa gccgaagtga atgctcagga
     3781 agccgagttg cgtcgcctga aagtgttgca atccttcaat cgcatcgttg ctccgtttga
     3841 cggggtgatc accgaccgga ataccgatat cggcatgttg atcaccaccg gcagcaatgc
     3901 ctcgattccg ccgctgttca agatcgccga tgagcacaag ttgcgtgtct atgtcgaggt
     3961 gccgcagaat ttcgccgatc gcatcgagaa caacatgaag gcgcagctgt acttccccga
     4021 tcgcccgcgt caggaattta cggccacggt ggtgagtacc tccgaagcca ttcacgtcga
     4081 ttcacgcacg ctgaccgtcg agctgatgat ggacaacaag ggtgaggaac tcatgcctgg
     4141 tgcttatgcc gacgtggcgt ttaccctgcc cacgccggag catgtgctac gtctgcctgg
     4201 cagtgcgctg ctgttccgcg agggtggcct caaggtggcg acgatcgggc ctgacaaccg
     4261 cgtggtgctc aagtcgatca aggttgcgcg cgatctgggc aagatcgtgg aagtgtcatc
     4321 cgggttgctg cctactgaca gggtgatcga tacgccttac gattcgattg ccgacggtga
     4381 ccgcgtgcgt attgcgaatg ggaaataagc catgaaactg aaccgagtgg cgctggtggt
     4441 cgcaggcatc ctcccgttgc tggccggatg ttcgcttgcg ccgacctata acgagcctaa
     4501 aaccgaagtg ccggcaacct acaaggaatc cggggtctgg caaccggcca gtccctccga
     4561 tcggatcggt cgcggcgact ggtggaagga ttacaatgac gccaccatcg acaatctgat
     4621 tccgaagctt gacgcggcga atccgacact ggccgaggcg gtaggccact atgaccaggc
     4681 gcatgcgctc gaacagcagg ccagttccag cctgtttccg gtgctgaatg ccggtgcctt
     4741 tgataccgcc aaccagcagt tctataacaa gccgcacatc gagacgccca atcccgcgat
     4801 cacctcgccg cagtcgtatc aggaacgtta tattggcgtt ggcgcctcgt atgaagtgga
     4861 cttctggggt cgggtgcgca atgaggttgc ggcaggcagg gccaatgaag ctgccgctgc
     4921 ggctgatctt gaatcggtgc gcctgagtct gcgcgcaaca ctggtcaatg actatctgtc
     4981 gctgcgtggc ctcgatctgc agatcaagct gctgtcggat gtggtggtga tgtaccagaa
     5041 agcgcttgat ctgaccgagt accgcttcca cggcggtatc gattcggaac tgagcgtatc
     5101 gcgcgccaag gcacagcttg attttgcccg tggcgagctg gccgatttcg tcgctgcgcg
     5161 cgcgctgtac gaacacgcca tcgcttcgct ggtggggcaa cccgcatcgt cgttctctct
     5221 gccttcggtg ctggtcgaac tgcaagtgcc gaacattccg gtgggcgtgc cttctgccct
     5281 gctgcagcgc cggcccgaca tcgcggctgc ggagcggcgt gtggccgcag ccaacgcgca
     5341 gatcggtgtg gtacgcgcgg cgttctttcc caccatcgat ctggttggtg gcatcggtca
     5401 ggacgcgatt ctggccaacc agttcaacct cgcgcctacc acgctctgga ccatcggacc
     5461 cactgcgttc ctgaccatat tcgatgccgg acggcgtcag gcgatcgagg atcaggccaa
     5521 ggctacattc aatgtcgctg gcgcgcagta tcgtcagacc gtgctcaggg cgtttcagga
     5581 ggtcgaggac aatctgaccc tgctcaacca gttggccgcg gagtcggtga gtatcgatgc
     5641 tgccaagggc gatacgcagc gtgcgctgga aattgccatg agccgctacc gcgaaggtat
     5701 ggagaactat ctggaagtgg tgacagcgca acagccggca ctgcaggccg agttgcagtc
     5761 gattcgactg catacacgca ggctgcaggc cagcgtcagc ctgatccgtg ccctgggcgg
     5821 cggttatcag cggccgaagt cctcctgacc ggcgggagcg ggtgcaacaa aaaggccatg
     5881 cgattgcatg gcctttttgt tgcacggcgt ccgcatcgtg aatgcgaacg ccctgtactg
     5941 cggtattact tggtggcggt gtacggcttg aaaccgaagt gttcgaagat ttccagcgct
     6001 ttgggcgagt gcagatagtc cacccacatt tgcgctgcct tcttgtgcgg agccgctttg
     6061 agcggtgtgg cagcgtaaat cgcagaaacg ttctgggcgt ccggaatgtt cacgtggctg
     6121 atcgggttgc cggtctcttc ctggaagatg gcttccgact gccaggtgac gccggcttgc
     6181 gcgcggccaa gcatcaggta cagcggcgat tggcggtgat gaatgtgggt caaaatggtt
     6241 tcgccgttct tgaccttggt gtcgtacacg gcggtcacca gtgcttcgcc accggccttg
     6301 gccagtgcgg ccttgatctg acggccaatg ccttcgaatt cagggttcgg catgaccagt
     6361 ttcacaccgg gtttgcccag gtcgttcagg ctggtgatgt gtgcaggatt gcccttgggg
     6421 atcatgatgg tcagcgtgtt ggtcgcatag cgtacggccg gagccttgcc ataaccttcg
     6481 tcgatggcct gggtgacgcg cttgaggccg gcggcgtaca cgtccgggtg cacggtccag
     6541 gtcatgttgc cgacggtgac ggtgccatgc ttcttgatct gctcgtagca caggccgggc
     6601 ggaatggttt cgtagtaaat cttgcctttg aattcaggat gctggcgttc gaattcgtgt
     6661 accagcgggg ccatggcgaa gtagtagttg ccgcccacca gtatcaccag cctggcgttc
     6721 atggggtcgc catggaaatc aggcaggttg tccacttcgg gaacggtgaa ttcgaggccg
     6781 cgatcctgca ccggattgtt ggcgccatgg ctccagggcg ggaagaactg ttcctgattc
     6841 gtcgtgtcgg ccatggcgga agcggcaaag cacgcctggg ccgcaaacgc gagtgcgact
     6901 gcatgcgaaa gaagggaacg ggatacgctc atcgggatct cctgttgtcg gaagcattgt
     6961 attgggtgtt ggacatttca gtgccgcacg tataatataa cccgcgctgc cggatgtaaa
     7021 ttagtgtggg cggttcgaga ccgatttctg tacgttttac gaaattgact gcgtgttgac
     7081 gtgcagattt ccgaaattat acaaaatcaa ggttgatctg acctgcccgg gagctaggtc
     7141 cggcaggagg gttcgcatta cacaggagat cttcaatgaa gttgaaaaca aaattgctgg
     7201 ctgttgcgct gtgcgcggcg ttcgtggccc ccgcctttgc tgatgacgct gcttcggcat
     7261 cggcttatgt gaagcccgcg tttacgcatg aatcctatgg caacctgaaa atcgtggtgc
     7321 cgctcaccaa tgaaatgctg ttgccgatga agttgcgcaa catcgccaat tcactcaagg
     7381 ccgctgaagc ctggggtggc aagctggacg tcaaggtggt gatgtatgcc aagggcgtgg
     7441 cctggctgaa gaccccggat gacaagctca aggcgcaact ggatatgctg cgtgcccatg
     7501 gcgttcagtt cgtcgtgtgc aacaacacgc tcatggaaca gggcatcaac tatcacaccc
     7561 tgtatggtgt gaccgatgcc gacatcgtgc cctccggttt tgccgaagtg gccttcctgc
     7621 aggctcgcaa gggttacgtg atcgatccgg ccatgtaatc gtgctgtggc tgcgacgccg
     7681 tgaaggttgt tgcagcggcg catgtccgat gatgacgccc ggccccggcc gggcgttgtt
     7741 ttttgaagcg tccggtacgg gggcgcggct gcggaaatgt gtgatgcgcg cactgtcgca
     7801 acaggacctg tgggcgatgg ctgcgtctcg atggcattcc ggggcggtgc cggatttatt
     7861 tcttgtcacc cactatggtt tcgtcctata atcagcctct tttcaatcaa ccggttgagt
     7921 gtagcaatgg tcgtgattcg tcttgctcgt ggcggcgcca gaaaccgccc gtattacaat
     7981 gtggtagtcg ccgattcgcg ctgccgtcgt gatggtcgtt ttatcgagcg cgtcgggttc
     8041 tacaatcccg tcgccaagga aggcggcgaa aaaatgcgtc tggacctggc tcgcgtgcaa
     8101 cactgggtag gtcacggtgc gcaggtgagc gatgccgtct cccgtctggt aaagctggaa
     8161 gaaagtgccc gcgtcgctgg atgacctggt ggtgatgggg cgcctgggcg ctccgttcgg
     8221 cgtgaagggt tgggttcatg tccagccata cagcgaaatg gacgatgctt tggccgatca
     8281 tgccgtgtgg caggtcggcc gtttgggcgt ctgggcttcc tacgaactgg agggctttcg
     8341 tccgcaaggt agcaagatgg tggcgaagct ggtgggtgtg gatgaccggg atgcggcatt
     8401 cgccctgcgt ggcagcgaag tcgcggtgcc gcgtgccagt ctgcctcccg ctgggcagaa
     8461 tgaatattac tggtccgacc tggtcgggct ggatgtggtg aatgtgcagg gcgaacgtct
     8521 cggttcggtt gaccatgtga tcgaggccgg ggcgcacgat gtgctcgtcg tacgaggcga
     8581 gcgcgaacgc ttgatcccgt tcgtggggca gatcgtgctg gaggtcagcc tggctgcacg
     8641 gtgcattacg gtggactggc aggcggacta ctgatggtcg tggcaaagca gatcgatgtg
     8701 atcagtctgt ttcccgacat gtttgacgca atttcccgac atggtgtgag cgggcgggca
     8761 ttcgagcgtg gcctggcgca tttgcagttg tggaatccgc gggagttttc caccaatccg
     8821 taccgcagtg tcgatgaccg cccctacgga ggcggcccgg gcatggtgat gcaggccgaa
     8881 ccgctggccg cagcgattga agctgcccag gcgcggcaac atgatgccgg cgtgggtgat
     8941 ccgcgcgtga tttttctcgc accacatggc cgtcggctgg atcatgacgc ggtcatgcgt
     9001 ctggcgagcc tcgatgggct ggtgttgttg tgtggacgct atgaaggtgt cgatcagcgc
     9061 ctgatcgacg aatcggtaca tgagtgtgtg tcgattggcg attatgtgct gtcgggcggc
     9121 gaactggctg cgatggtcgt gatcgatgcg ctgctgcgtc agttgcctgg tgcgctgggt
     9181 gatgcgcaat cggccgatca ggattccttt gtggatggcc tgctcgatta cccgcactac
     9241 acccggccgg aagtgtggcg tgggcggcag gtgccgtcgg tgctgctggg cggcgaccac
     9301 gaacgcatcc gtcgctggcg cttgcagcag tcgcttgcgg acacagcagc gttgcggccg
     9361 gatttgctgc gcaatcgcga tttgagcaag gaagaatcgg tcctgttggc ccagagcctg
     9421 caggcagaac agccggagtg atccggcgat aacaaccgtg cgtgtcaggt ggcgacctgc
     9481 tccgcgcacc ccggtcgtga ccggatatct gtccaaggag aaacgcatga atatcattga
     9541 acaactggaa caggaagaga ttgcccgcct gggcaagaac attcccgatt ttgctcccgg
     9601 tgataccgtg gtggtgagcg tcaaggtcaa ggaaggttcg cgtgagcgtc tgcaggccta
     9661 tgaaggtgtg gtgatcgccc ggcgtaaccg tggcctcaac tcggcattca tcgtgcgcaa
     9721 gatttccgcc ggagaaggcg tggaacggac gtttcagacc tactccccgc tgatcgacag
     9781 tatcgaggtc aagcgccgtg gtgatgtgcg ccgtgcaaaa ctctattatc tgcgtgagcg
     9841 ttccggcaag tcggcgcgta ttcgtgagaa actgcctgcg cgcaaggtgg ctgcaacggt
     9901 tgataccgcc gcagaatgaa acgacggttt cgccaggcat gaaaacgccg cgcattgcgc
     9961 ggcgtttttt ttggtgcaga tgcgggactg gccgcctgga cgggtgcagg tgcgcgcaca
    10021 ggctcacatg gcgcgaaaga tgccgatgcc gtgatcggaa tgcatcatga ttgcccgttt
    10081 tcgagcgcca gacgttccat ttcgagccat ggatcaccgc cgtccagccc cttgatcatg
    10141 cggtcgatgg cggcgagacg ttgcatggca gcgtgcacct gaccaggctg caatcggcgt
    10201 aatgcacgtt cgaacagcga ctggcgctgg tcgcggatgc ccagttcgcg catgcgacga
    10261 ccgcgttgct cgggtgagct gtcggccaga cgcgcgagcg accgcaattc gcgagtgatg
    10321 gcccacaata ccagcgtggc agcctcacct tcgtcgtgca gcccatgcag tatgcgaatg
    10381 aggcggcggg catcgcctgc cagcaaggca tcggtcaggt cgaatacatc gaaacgcgcc
    10441 acgtcgagca cggcatcacg cacctgcgtt gccgtcagta ctccaggcgg atagagcagt
    10501 gccagtttgt cgatttcctg ctgcgctgcc agcagattgc cttccacctt gtcactgatg
    10561 aagcgcaagg actgggcatc ggtgcgttgt ccgctccggg ccaggcgttc tccaatccag
    10621 tgcggcagac gttcggtggc gggggtgaat acggcaacgc tgacgccggc gtcttcgagc
    10681 gccgtaaacc acttgctctg gctcacggcc cggtctggtc gaggcagcac gaccagcgtc
    10741 acggtatcgg gcggcagtgt cgcgcagtag agttgcagcg ctttggcgcc ttcggtgccg
    10801 ggtttcccgg agggaatgcg cagctcgacc agtttgcgtt ctgcgaatag cgacaggttg
    10861 cgtgaagcat caagcagtcc tgaccagtcg aaacgtccct cgacaacgaa gagatcacgt
    10921 tggctgcagc cggactcgcg tgcgcgctgt cgcaccagat cgcaggtttc ctgcaccagc
    10981 aacggttcgt cgccatgcac gacatacagt gccgtcaggt tgcgcgccag ctgcgcgggc
    11041 agctgttcgg ccttgatgcg catgacggtc agtgcagcat gaacagccgg tgcaggatct
    11101 ggtgcgcggc gtcgttctgc atgtcgctgt tgagaaactg ttgttcgttg ccgtaggcaa
    11161 acggctggct tggattgaag ttcatgttgc gggtgaggaa gatttcctgc ggcgcggcca
    11221 cttccttgtg atcgggcgtc agtatccggt atttgacccg atactgcagc tggtattcac
    11281 tgaccgtgcc ggccccggta atggcgatga tcagcatctg gttggtttca tccatgatct
    11341 ggatgatgcg ttcggccttg gcgggcgaag atgccagccg gtctgcgtga ccgccggccg
    11401 tcatgaggcg gcgcagggct gcggcgacgg ccgagccgtt gccactgtcg atatagatcg
    11461 aagtgtacgg aatgtcggtc tggttgcgca ggtggaaacc acaaccggca agcgcaaggc
    11521 ttgcaacgac aaccagggcg gacagcagca ggcgcaaaga ctgggtcatg ggcagggggt
    11581 cctgaaagac gcgtggtcac accacgatat taaccaggcg gcccggcacg acgatgatct
    11641 ttttcggtgc ctgcccctgg acgaattttt gcactgtcgc gtcggccagt gcggcctgtt
    11701 cgatgtgggt acgctcggtg tcgcgtgcta ccgagatctc gctgcgcagt ttgccgttga
    11761 cctgtaccat cagcgtcagt gtgtcctgtt gcagcgcggt cggatcggtc tgcggccagc
    11821 cttgcgcctc gagcgcgctg ccagggcaca gttcgttcca cagtgcgtcg cagatgtgcg
    11881 gtacgatggg cgagagcacg atcaccatgg cctgcagcgc ttcctggatc acgctgcgtg
    11941 tgaccggatc gcgcgcttcg attctgccca ggctgttgag cagttccatc acgctggcga
    12001 tggcggtgtt gaaggtctgg cgtcggccgt agtcgtcgcc gattttctgg atggcgctgt
    12061 gcagctggaa tcgcagtggt ttgagcgacc cgggcaggtc gcccccctgc catgcggcaa
    12121 ccggtccggt tgcgacgtga tcggcgcaga aagtccacag gcggcgcaga aagcggtgtg
    12181 caccctccac tccggcatcc gaccattcca ggctttgttc cggcggtgcg gcgaacatgg
    12241 tgaacaaccg tgcagtatct gcgccgtagc ggtcgatcag ttgttgtgga tcgacaccgt
    12301 tgttgcgcga tttggacatc ttttcggttc cgcccagcat gacaggctgg ccgtctgcgc
    12361 gcagcgtggc atgggtgggg cggccgcgtt cgtcggtggc gagatcgatt tgtgccgggt
    12421 tgaaccattc gcgtttgccg ttcggctgtt cgcgatagaa ggtgggcgcg atcaccatac
    12481 cttgtgtgag caggcgctcg aatggctcgt cgctgtgcac caatccctgg tcgcgcatca
    12541 gtttgtgaaa gaagcgtgcg tacagcagat gcaggatggc gtgttcgatg ccgccgatat
    12601 actggttgac gggcagccag tcgtcggcac ggctgtcgag catggcgctg tcgcaatcgt
    12661 ggctggcgta gcgcgcgtaa taccaggacg attcgacgaa cgtgtccatg gtgtcggttt
    12721 cccgccgcgc aggacggcca caggtcgggc agttgcaggc atggaattcg ggcattctgg
    12781 ccagcggcga gccggcgccg gtgaccgtga cgtcctcggg cagcaccact ggcagatgtt
    12841 cggccggcac aggcacgtcg ccgcaatggt cgcagtggat aatgggaatc ggacagcccc
    12901 agtagcgctg gcgcgagatg ccccaatcgc gcaggcggaa ctgggtgcgc gtgccgccga
    12961 ggccgcgccg ttcgagttcg ccagcaacgg tggcaaatgc ggtttcgaaa tcgaggccgt
    13021 ccaggaatcc ggaattgacg catacgccgt gttcggcata gcgttcctgc cacacgttat
    13081 caacagtgct gtgcgcaccg tcggctgcgg caatcacctg acgcatgggc agtccgtagc
    13141 gtgcggcgaa ttcgaaatcg cgttcgtcat gcgctggcac ggccatgact gcgccttctc
    13201 cgtaccccat aagcacgtaa ttggcgaccc acaccggaag tgcctcgccg gtaagcgggt
    13261 gggtcacata cagaccggtg gccatgccac gtttttcctg ggtggcgaca tccgcctcgg
    13321 ccacgccgcc acggcggcat tcggcgatga attcggcaag cgcagggtta tgttcggcgg
    13381 cctgtgccgc cagcggatgc tcggctgcga cagcaacata ggtcgcgccc atgagcgtgt
    13441 ccgggcgggt ggtgtagacc ttgagcacac cgtccgcacc gactgaatcg cgctgccagg
    13501 gaaaatgcac ttcgcagcca cggcttttgc caatccagtt gcgctgcatg gtcttgactt
    13561 gttccggcca gcccggaagc gtgtcgagcc cggcgagcag ttcttcggca taggcggtga
    13621 tacgcatgaa atacatcggt atctcgcgtt tttccaccag ggcgccgctg cgccacccgc
    13681 ggccgtcgat tacctgttcg ttggccagta cggtgcagtc gaccgggtcc cagttgacgc
    13741 tggcggtctt tttgtagatc agcccttttt tgaacagctc ggtgaacagc cattgttccc
    13801 agcggtaata ctccggctgg caggtggcca gttcgcgttg ccagtcgatc gacagcccaa
    13861 gccgcttgag ctgggcgcgc atgtggcgaa tgttgtcata ggtccagctg gccggcgcga
    13921 cctggttctg gatcgcggca ttttctgccg gcagaccgaa ggcgtcccag cccatcggct
    13981 gcagcacgtt gtaacccttg agccggtgaa agcgtgccag tacatcgccg atggtgtagt
    14041 tgcgcacgtg acccatgtgc agcttgcccg aagggtaggg aaacatcgac aggcagtagt
    14101 agcgtggcgc atcgccgtcg tcgcgtgcgt ggtacgtgcg ctgttcgtcc cagcgttgct
    14161 ggaggtcgag ttcgatgtgt tgcggatcgt agtgcgtatt catgcggtcg tgccaggctg
    14221 cggaagaggc aattatcgcg gaaatgggcc gcgcgcgcca gcaatggcga tggcgatggg
    14281 cgcggtgcag gccgtccgcc gctgcgtcgc ggtacggttg tcacaaaaca ttatcaattg
    14341 ttatcaatat ataaattaaa ataaactgta ttgaattcaa tttgatacgc aaattacgca
    14401 gtcatttttg gtgctcaagg agaaaacatg aagattcgtc ttatgctgtt ggccgtgttg
    14461 gcgagcccgg gtctggcgca ggccgacgca tcgctcgatg ccgtcggggc ggtaaacaac
    14521 gaaattggcc tgaacctgat ttcgacgcac atgaattaca gcgaaaccgg gaatggctcg
    14581 gtgggtcccg ccggcgccga actcgatagt gaggatggcc atgtcggcgg attcgcgctc
    14641 gatgcttcgc tgatgcagga catcctgttc gggcgcgatt atgtcgcgct gggatacgat
    14701 cgggttcatg gctcggtgac ctataccggg gctaccggtg cggacccggt ttacggttcg
    14761 gcggtcgaaa agagcgatga cacgacggat aatttccatt tgcgctgggg acgcggtttt
    14821 gccgtgcagc actggctggt gactccgtat ctcgaactgg gacgccacgc ctgggatcgc
    14881 gttgtcgccc cgggattggc cattggcctg acggagaact acacgcacga ctactatggc
    14941 atcggcacca tggtgcaggt ggctccggtg cgcgcattgg tgctgacgtt gcgcggtctg
    15001 gctggccgca cgtttgatgc cggaatgacc gcgctgggtc agaacctgtc gctcggtgat
    15061 tccgcactgt accagatcgg cttgcacgcg gattacgcca tcatgccggc ggtgcatttg
    15121 cgtgccggca tcgactacat gcaattcgat tatggcgctt caccgtgggt aacggatacg
    15181 ttgtacgggg gatggatcgc cgagccggac agcagcaccc ggctgaccac catcgatgcc
    15241 ggagtggcct acagtttctg agatgccgca gtggtatggt cgtgcccggc agcgtgctgc
    15301 ggcggttgga cagcgtggcg acggaaagct atactagcgg gtcaagaata cgctcgattt
    15361 taccttccga atggagtcat catgtccaag aaacatcccg tgatcgccgt aaccggttct
    15421 tccggtgcgg gcacgaccac cgtcaaacgt gccttcgagc atattttccg ccgtgaaaac
    15481 attcatgctg cgatcatcga gggggacagc ttccacagcc tgtcgcgtgt ggagttccgc
    15541 gaccgggtgc gtcagtccga agccgcgggc aacaagcatt tcagccactt tggccccgaa
    15601 gccaaccatt tcgacaagct cgagcaactg ttcaaggcct atggc
//
LOCUS       DAIANL010000149        10965 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1817207, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000149 DAIANL010000000
VERSION     DAIANL010000149.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 10965)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10965)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10965
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817207"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2153 a         3738 c         3015 g         2059 t
ORIGIN      
        1 ccctgccgca ggagatcccg gtcacgttgc cctccgtgct ggtgcgccag cgcccggata
       61 tcgtggccgc cgaggccagt ctgcatgccg ccagtgctgc catcggcgtg gccaccgcgg
      121 cgctgtatcc ggcattcaca cttgatgcgg gctatggcca gaacaacact tcattcggca
      181 atctgctgca gagcaatgcc acgttctgga gtttcggtcc cgatgtcagc ctgccggtgt
      241 tcgatggcgg aacgcttcgg gccaaacagg cagccgcctc agccgggtat cgtcaggcgc
      301 tggccagcta ccggcaaaca gtactggcag cgctggggca ggtagccgat gcacttcgcg
      361 gcgtacagca cgacgcagaa accgggcagg ctgtatttgc agcgcgtcac gccgcacagg
      421 ctacactgca tctgttgcag gtcaaccacc aggccgggct ggtcagcagc ctgcaggtca
      481 tcgccgccga ccttcagtac cagcaggcca ggctgacatg catccagatc caggcccaac
      541 gtctgcagga caccacagcc cttctggtcg cactcggggg ctggtggaac ctgcccgcct
      601 gattggcgca cgtcgccgga ttggtggatg ggggaccaag gacgcaggcc cactgtcgtg
      661 gagcgggcca caacatgcaa aaggccgccc gaaggcggcc ttttgcatgc ggcaaacccc
      721 ggcaggaatc agcgcttgga gaactgcttg gcgcgacgcg ccttgcgcag accgactttt
      781 ttccgttcga cttcacgcgc atcgcgggtg accagaccgg ctttcttcag ctgcagcttg
      841 agcgtttcat catactcgat cagcgcgcgg gtaatgccgt gtcgtactgc accggcctga
      901 cccgtttcgc caccaccatt gacgttgacc atgatgtcaa acgccgacag attgctggtc
      961 agctcaagcg gttggcgcac catcatgcgt ccggtttccc gcgagaaata ttcgtccagc
     1021 ggacgctcgt tgacaacgat cttgccgctg ccggccttga tgaacacgcg agccaccgca
     1081 ttcttgcgac gacccgttcc gtaatagtaa tttccgatca tggcttatcc cctgatttcc
     1141 aggactttcg gctgctgcgc agcatgcgga tgggttccgc cagcgtagca cttgagtttc
     1201 ttcagcatgg cataacccag cggccccttg ggcaacatgc ccttgacagc tttttccagc
     1261 acgcgaccgg gaaagcgctg ttgcatgcgc gtaaagttgg tttcgtggat tccgcccggg
     1321 aaaccgctgt ggcggtagta tttcttgtcc agcgccttgt tgccggtaac cttgagcttt
     1381 tcgacattca ccaccacgat gaaatcgccg gtgtcgacgt gaggagtaaa ttccggcttg
     1441 tgcttgccgc gcaggatacg cgccgcttcg gccgccacat ggccaagcac tttgtcggtc
     1501 gcgtcgacga cataccattc acgcacgact tcatgcggtt tggcggaaaa ggtgttcatt
     1561 cgaaacctcg gcgaattctt gtacagggaa agccgcgcat cataatccaa aacgcccccc
     1621 gtgtcaaaca caaaaccggc gtatcgccat atccgcatca tacgacatac acctgcatca
     1681 aattgccaaa tcacgcgcca tgtattacat tcaacgacaa tcgcttcact tcccccgcac
     1741 tccatgccca tccagatcaa aacccccgaa gaaatcgaaa aactccgcat cgccggtcgt
     1801 ctggctgccg agttgcttga ctacatcacc ccattcgtcc agcccggtgt caccaccaac
     1861 gaactggacc ggctgtgtca cgactaccag gtcaatgtgc agcactccat cccggcccca
     1921 ctcaattacg cgccaggggg gcacatgccc tacccgaagt cgatctgcac ttcggtcaat
     1981 cagcagatct gccacggcgt gccgaacgac aagccgctga agaacggcga catcgtcaac
     2041 atcgacgtca cggtcatcaa ggacggctgg catggcgaca gcagccgcat gtactgcgtc
     2101 ggcaatgtga gcccgcaggc acggcgactg tgcgacatca cctggcaggc actgtggcac
     2161 ggcatcgccc aggtacgacc gggcgccacg ctgggtgaca tcggtcacgc catccagaca
     2221 tttgccgaaa cgaacggtta cagcgtcgtt cgcgaattct gcggccacgg tatcggtcgc
     2281 aagtttcacg aagaaccgca ggtgctgcac tacggccgtc ctggcagtgg agtacggctg
     2341 caaccgggca tggtattcac catcgagcca atgatcaatg ccggccggcg cgaaatccgc
     2401 cagatgcctg atggatggac cgttgtcacc cgggaccgca gcctgtccgc gcaatgggag
     2461 cacatgctca ccgtcaccga aaccggttat gaggtgctga cggtatccgg acacacgcca
     2521 cccccgccag atttcatcac cgcaacagac gcaccgcccg ccccgtgaac gctcccgagc
     2581 ccgtcctgcc tggccactgg cgcgacaagc tgcgcgctga tcgtcagtca ttgcaacagg
     2641 cgttcatgaa cggcctgccg gtacgccagc tgctggcgcg acagtgcagc ctgaccgacc
     2701 gcctgctgcg cgacctggcc cggtcgatcg gcctgcctgc caacgtggcg ctggttgcag
     2761 ccggaggcta tggccgcagg gaactgttcc cctggtcgga cgtcgacctg ctcatcctgc
     2821 tggagcgcgc tccttcgcca gaagaacagg cggtgatcga acgtctgatc gggctactgt
     2881 gggatgtcgg cctcgaagtc ggtcacagtg tgcgtaccct cgacgaatgc ctcaccgagg
     2941 ccgaccatga cctcacgatc cagaccaacc tgctcgaatc gcgcctgctc ggtggccatg
     3001 ccgcgctgta ccaccgcttt cgccacgagt ttcttgcccg tctcgagccg cgcagctttt
     3061 tcgaaggcaa actgtcggaa caggaccagc gtcatgcacg ctataacgac agcgcaagcc
     3121 aactcgaacc caacatcaag gaaagtcccg gagggcttcg cgacctgaac aacctgcgct
     3181 ggctggccca ggccaccggt atcggcagcc actggcgcga cctgacccac gccggcctgc
     3241 tcaacccgac cgaactgcgc cagttgcaac gtctggaact gcgcctgttt accctgcgcg
     3301 cgcagctgca ctacctggcc ggtcgccgcg aagacaggct gctgttcaac taccagcacc
     3361 aactggcgga gcagtacggc atcgaatcca ccaccacccg gcgtgccagc gaactgctga
     3421 tgcaacgcta ttacctggca acacggttcg tggcacagat gcgcgatatc ctgctgccca
     3481 gcctgcgcgc ccgcgcgttc ggcaaccatc agctgccgct ggtgccgctc aacgaacgtt
     3541 tccaggtacg cgacaatctg ctcaaggcca tcgacgacca gctcttcgaa cgtgagccac
     3601 atgccatttt cgagagcttc ctgctgctgc agcagcacgc cagcctgtcc ggcatgagcg
     3661 catcaaccct gcgagcgctg tggcgggccc ggctgcgcat ccgtcaggca ttccgccgcg
     3721 acccggtcaa ccaccagctg tttctccaga ttctgcgcac accccgtgta ctgacgcgta
     3781 cgttgcgtgc catgaaccgc tacgacattc ttggccgcta cattccgatg ttcggccgca
     3841 tcgtcggcca gatgcagcac gacctgtttc atatctatac ggtggatgaa catattctca
     3901 ccgtgctgcg caacctgcgt cggttgacga tccccgaact cgcacacgaa taccccatgg
     3961 ccagccggct gatcggcggt ttcgacaagc ccgaactgct gtatctggcg gccctgtttc
     4021 acgacatcgc caaaggacgt ggcggcgacc actcggtact cggcatgacc gatgccagac
     4081 agttctgcca gcagcatgag ctggcgccag aagacaccgc gctggtcgta tggctggtgc
     4141 gcgagcacct gtcattttcc gctacagcac aaaagcagga cctgtccgat ccggacgtca
     4201 tccagaaatt cagcgagcgc atccctgacg aacgccatct gaccgcactt tacctgctca
     4261 cggtgtgcga tattcgcggc accagtccaa cggtgtggaa tgcctggaaa gccaagctga
     4321 tcgacgacct gtatcatgcc acgcgccgcc acctgatcgg cgatgcgcca ccgcgttccg
     4381 gcatcgaccc ggtcaagcag gaagcctcac gcctgctgcg tctgtacggc ctgtcaccgc
     4441 acaccgaaga cgcgctgtgg aaccagctcg acgatgccta tttcatgcgc aacgagccgc
     4501 aggaaatcgc ctggcactgc cgccagctgt ggcacagcct ggacaaaaag cagcccatcg
     4561 tccgcgcgcg ccccagcccg gctggcgaag gcatccagac catgatctac acgccggatg
     4621 aacccagcct gttcatgcgc atctgcgcat ttttcgatca gatccagtac accatcgttg
     4681 atgcacgtat ccacaccaca cgaagcggct atgcgctcaa cagctttgtc gtgctcgacc
     4741 cgatgcggcg cgaagtcgct taccgcgact tcctcaacta catcgagcat gaactgaccg
     4801 cccggctgaa cgcggctgaa ccgcccagaa ccgtttcacg cggacgcatc agccgccatc
     4861 tcaagcattt cccgctcgat gccgaagtcg tgctgcgcgc ggacgaaaag ggccgtttct
     4921 atgccctgca cctgaccgcc ggggatcgac ccggcctgct gttcgccatc tctcgcatcc
     4981 tgttccagca ccaggtcagc ctgtattcgg ccaagatcag cacactgggc gaacgcgtgg
     5041 aagacacttt cctcattcac ggcgaagcgc tcgaccagcc ccagtcacgt ttgaaactgg
     5101 aaaccgacct gctcgaagtg ctcaacctgt agccgggcga cacaacccct gtgtgcgaaa
     5161 aaacggcagg gggtggcgca catccccctg tgtcatccgc cgcgtggccg ggccgctcag
     5221 tcgcaacgct gatcgagata tgccttgaaa tcggcagcca gttcgggatg ctgcaatgca
     5281 tgctccaccg tggccttgag gtagccgagc ttgctgccgc agtcataacg cacaccttcg
     5341 aaggcatagg acagcacctg ctgttcgtgc agcagacgcg cgatcgcatc cgtcagctgc
     5401 aattcgccac ccgccccgaa cggaatgctc tccaggtggc tgaatatgcg tggcgtgaga
     5461 atgtaacgtc cgaccacgcc cagagtcgaa ggcgccaccg cgggcgcagg cttctccacg
     5521 atctgctcca ccttgcccag cgaaggcgtc atggtctgca ccgccacgat gccataggat
     5581 ccggtttctt caggggccac gttctgcacg ccgagaatcg aacactggta atagtcgaac
     5641 agcgaggtca tctgtttcat cgccggctgt acggcatcga tcaggtcgtc cgcaaggatc
     5701 acggcgaacg gttcattgtt gaccacaggc ttggcgcaca gtacggcatg ccccagtccc
     5761 aaagcctcgg cctgccggat atagatacag tttaccccct gcggcacgat gttgcgcacc
     5821 aggtcgagca acgccgtctt gccccgcgcc tccagttcgg tttccagttc gtaggctttg
     5881 tcgaaatggt cgggaatggc gcgcttgttg cgtccggtaa tgaaaatcag gtcggtgatg
     5941 cccgctgcca cagcttcttc cacggcatac tggatcagcg gtttgtccac gatcggcaac
     6001 atttcctttg gcatggcctt ggttgccggc agaaagcggg tgcccatccc gcccaccgga
     6061 aataccgcct tggtcacttt atgcattgtg tctgtctcct gagttggaat gcagccattc
     6121 aaaaaaccgt tcctcgctcc acaccggaat atccagtgca tgcgcctgtt ccagcttgct
     6181 tccagcctgt tcgccggcca gcacaaaagc ggtcttcttc gacacgctgc ctgccacgcg
     6241 accaccggac tgctcgatca acgcccgcgc ttcgtcgcgc gtccagcgcg acaacgtgcc
     6301 ggtcagcacc accgtctggc cggcaagcgg caatgtcgcc tcggacaggg ctgccttgcc
     6361 ctcgcaaatc acgccggcag cctgcaaccg ctccagcacc gccagattat gtggttcggc
     6421 aaaaaactgg cgaatcgaac ccgccaccac ctcccccaca tcgcgtaccg caagcagcgc
     6481 cggctcatcc gcatgcatca atgccgcaag cgtaccaaag tggtgcatca gatccttggc
     6541 cgtctgttcg cccacatgac ggatgcccag cgcatacacc aaccgggcac acgaacggtg
     6601 ccggctggcg tcaatcgcct gcgtcaggtt acgcgccgat ttctcgccca tgcgttccat
     6661 cgcggccagc cgctccggct gcagggtata caggtcggca gcgtcatgca ccagttcgct
     6721 gtcgaccagt tgatcaacca gcttttcgcc caacccttcg atgttcattg cccgccgact
     6781 ggcaaagtgc cacagcgcct gtttgcgctg cgccgcacag aacagcccgc cggtgcagcg
     6841 agcaatggct tcgccgggca agcgcgcgac atgcgaaccg cataccggac aactcgacgg
     6901 catggaaaac tcgcgtacgt cggcccttcg ccgcgacagc accaccgaaa cgatttccgg
     6961 aatcacatca ccggcacggc gcacgttgac ccaatcaccg atgcgcacgt ccttgcgtcg
     7021 gatttcatct tcgttgtgca atgtagcatg gctgactgtc acaccgccga cggataccgg
     7081 ctccagtacg gccactggcg tcagcgcacc ggtacggcct acctgcacct cgatacccgt
     7141 caggcgcgtg gtggcttctt cagcaggaaa cttgtgcgcc agcgcaaatc gcggagcgcg
     7201 cgctacatag ccaatctgtc gctgcatggc cagcgagttg accttgtaca ccacaccatc
     7261 gatgtcaaac ggcaacgcca ggcgcgtcgc cccgatgcgg cgaaaatagc caagcaatcc
     7321 gtccgccccc tcgaccacct cgcgctcatg cgccactggc atgtgccagc gcgcgaggcg
     7381 atccatgaca gcccagtgcg tttccggcag gtcccagcct tcgcactggc ccacaccata
     7441 ggcataaaaa tacagatttc gactggcggt aaccgtcgga tcgagctggc gcagtgaccc
     7501 ggccgctgca ttgcgcggat tggcaaacac cttgcctcca gcctcggcct gcacccggtt
     7561 caaacgctcg aagtcgcgct tgagcatcag cacttcgccg cgcacttcca cccgcgccgg
     7621 ccactcgccc gcagccaact gcatcgggat cgcacgtacc gtacgcaaat tagtcgtcac
     7681 atcttcacca acgtagccgt cgccacgtgt cgcaccgaag accagcaccc cctgctcgta
     7741 caccaggctg actgccaacc cgtcgaattt cggctccact gcatattcca cgcggtcggt
     7801 gcccgtcagt tcgcgcacac gctggtcgaa ggcaaacact tcatcgtcac tgaatgcatt
     7861 attgagcgac agcatcggca tgacgtgcgt caccgactga aatccctgtg ccgccggcgc
     7921 ccccacccgc cgggtgggtg aactgtcgac agccagttcc ggatattgcg tttcaagttc
     7981 aaccagtcta cggaacaagt ggtcgaattc ggcgtcgctg acttcgggtg cgtcgagcac
     8041 gtagtaccga taattatgat attcgatctg ttcccgaagc gccgcagcct gcttgacgac
     8101 agattccgga atcatgcacg cacctagttg aacaggcgca gcgcccggcg accgcccgcc
     8161 gggatctggc tggcttccat ctgctcgtgg atgtgcacca gctgggtctg gatcttttcc
     8221 agaccggcct gcgaaagtgg gcgaatgttg tcatcgacca gacgaccgcc cagccgggcc
     8281 gccatttgct gactgaactt ggccatctcg gcaaacacgg ccacgccgtt ttcaatccga
     8341 ggcacttcaa acagcaacgt caccccatgg gtgaccaaca cgcccagctt atcggcagta
     8401 aatggagcat cctcatgatt gcacagggtg tagagcacat cgccacgctc gttgcgccgc
     8461 tggtagacac ccgtggcatc gagcgccatg cccgcttcct gcgccacctc ggcaatagtc
     8521 gcgccactga atgcctgcgc atccggactg accacgttca ggccaatgag cacatccacc
     8581 tcgacgcaga accggtccag ccgggccgcg cgctgcccgg ctgcggtcac gtcctcccaa
     8641 ctggccatgc catgctggcg ttcggccagc acgcgcacgt cttcgcagat ggcccgcagc
     8701 gccgactcgc tgacagctcc ctggcgatcg gcaagctgca ccgccaccag cacatcggta
     8761 aattcctggt tacgccatgg gctgatttcc acccatttgt cctgcagttc agcatacccc
     8821 atccaacgga cattccgtcc ttcggcacgc tgttgctcga tgagcgcgct aaatgccgtg
     8881 cctgccaccg gttcggcaac ccgcatgacg ataccgaatt cgagcagggc gtccagtggg
     8941 gccggcggca gttccaccgg atgggtggcg acggtttccg catgaatcgg cgcctcgggc
     9001 atcaccgcca ctggcggttc catatcgggc agcggctccg tttccacatc agcggtcatc
     9061 tcgtgatggt cacccatgac cggctcgatc cgcgcggcgt tgccagtcac ctccgctgcg
     9121 ttggtctgca ggtgatgctc gacgtagggt tctccggtcg tgccggtcac gattggcgtc
     9181 tgcgtgtctt gtgcatgcag ccccagaccg gattcgcgca gcaccggttc gctccatgct
     9241 tcctcgtcca cgtcggcagg aggcgaacca ttatccacct ggacggcatt gcggccgaag
     9301 gtcgccatcc actgcttgcg gtatttacgt tcctgccacc agttgtgcaa caccaacccg
     9361 cccaccgcga gcacggccac cacaatcagg ctgatctgca atgcactcat ttctctcttc
     9421 caacttcccc gacccgaaca gcaggggttc cggccggggt cgcatccaga atctggaaat
     9481 acacttcatc cttgcgcacc atgcccaact cctggcgggc gcgttcctcg atcgcgccaa
     9541 ccccctgctt gagatcgcgc acctcggcag acatggcggc attgcgttcg gccagctgcc
     9601 tgtctaccga caactgatca gcaagctggc gccgggtatg ccatacctgc ggccagcacc
     9661 ccgggccgat ccagagcgga tactgcaacg ccagcaacag cgtgatcagc actccggtca
     9721 accaccgcat ggtcagcccc gtcccgtatc agctcaggag atctgataaa acgcggaacg
     9781 tccggggaag cgcgccatgc tgcccagctc ttcctcgata cgcagcagct ggttgtattt
     9841 tgccagtcgg tcggaacgcg acagtgaacc ggtcttgatc tgtaacgcat tggtcgccac
     9901 ggcgagatcg gcgatgaagc tgtcctcggt ctcgcccgaa cgatgcgaca cgacagcggt
     9961 atagccggca cgacgcgcca gttcgatggc ggtcagggtt tgtgtcaggc tgccgatctg
    10021 gttgaccttg atcaggatgg aattggccac gccgcgcgcg atgccttcgg ccaggatctc
    10081 ggcattggtg acaaacaggt catcgccgac gatctggatc gacttgccca gacgcccggt
    10141 cagggtcgcc catcctgccc agtcgctttc cgccatgccg tcttcgatac tgatgatcgg
    10201 gtatttgtcg caccatgccg ccagataatc cgtcatctgg ccggaatcca gcaccagacc
    10261 ttccgattcg atgtggtagc gaccatcgcg atagaattcg cttgctgcgc agtcgacggc
    10321 aatggccaca tcgcgcccgg gcacgtaacc cgccttttcg atcgcctgca cgatgagttt
    10381 cagcgccgcc tcgttcgatt cgagattggg cgcgaacccg ccttcgtcac ccacggtagt
    10441 agccataccc aggccgtcga tcagcttttt cagggcatga aacacttcga ccccgtatcg
    10501 cagcgcttca cggaaactcg gcgcgcccaa cggcacaatc atgaattcct gcatgtcgat
    10561 gttgttgttg gcatgcgcgc caccattgat gatgttcatc agcggcacgg gcagcgacat
    10621 cgggcctgcg ccgcccaggt agcggtgcag cgacaatccg gaagcagccg aagccgcccg
    10681 cgccacggca agcgataccg cgagaatggc gttcgcaccc agccgcgatt tgtccggtgt
    10741 gccgtcgaga tcgatcatga gctggtcgat gaagccctga tcttcgacgt ccagaccgat
    10801 cagcgcctcg ctgatttcgg tattgaccgc ttccaccgca cgcagcaccc ccttgccctg
    10861 gtagcggttg gcatcgccat cgcgcaactc gatcgcctcg cggctgccgg tggacgcacc
    10921 ggacggcacc gccgcccgcc ccagttcgcc cgactcgagc agcac
//
LOCUS       DAIANL010000150        17792 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_393055, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000150 DAIANL010000000
VERSION     DAIANL010000150.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 17792)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17792)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17792
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393055"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3813 a         6137 c         4805 g         3037 t
ORIGIN      
        1 ctgctgttcc gctaccgcgc acgcaatttt ccgcacacgc tgaatgagga agaaaacgcg
       61 cgctggcacc gtcactgcac cgcccggcta ctgcatggcg aacagggtgc acgcagcctg
      121 caacaactga gcacgcagat cgacgcactt gcggaaagcg ccgacgaacg tggcgaagaa
      181 attcttggcg atctgtacga ttacgccgaa accctgatgg aacacatctg actagacatc
      241 atggtcgcca gccggtagca ggtggccagc atgtggtaac caatccacgg atatgagcat
      301 gattcatcgt cgtagccaga cttcgcatcc cagggtcgat caggatttat tgacttttgc
      361 gaccatatga gcaggccgtt gaaacaggta gcgaggaccc ccggatgcaa ggcgcgagaa
      421 acgcaacgac gagacctatc aaatagatcg gcgtgggagt gagtatcgca gcaacgccgc
      481 agacgggtgc cgcaggactt tttcaacggc ctgctagcgg gtctgacgca cccgctcgaa
      541 cagacacacg gccagcgctg cagcgacatt gagcgattcc atccgtccag gcatggggat
      601 gcgcagcgac gcgctcactg cggcacgcag cgccggcgac aggccggcac cctcgttgcc
      661 gaacacgaac gccagcggtc cacgcagatc ggcctgatac aggtcctcag cggcatcgag
      721 cgccgtcgca catacaagac cgggaaaatc acgtgccact gcaaccagat cagcctgttc
      781 gacaatccgc aatgcgaaat gcgcgcccat accgccgcgc aaggtcttgg gcgaccaggc
      841 atcagcacac tcgtccgaca gccacacgct gtccacgccc gctgctgccg cactgcgcaa
      901 cattgcaccg agattgccag ggtcctggat gcgttcaagc agtaccgcaa aatgcgaggc
      961 ctgctgcggc cacacgggcg gttgcaccac cgccataact ccggtagggg ttctgacggg
     1021 cgaaatggcg tcgaacaatg gccgggtcag cagaacgacc tcagcctgcg gacaacgctg
     1081 ctgccaggct gcagcatccg gttcctcgca caccagcacc tgcaacggta cgccaccgct
     1141 cgccagaaat gcttccagca gatgctcgcc gtccagcagc gccaagcctc ttgcacggcg
     1201 ctcgcccgcc gattcggcca gcaatttcca gccgcggtaa cgggaattct gtctggaagc
     1261 gatccattcg cccatcaggc gcgacgccag agcgtcgcgc ctggctgatc ctcgagcacg
     1321 attccggcct gcatcagttc ggcgcgcaca cggtccgcct cggcaaaatc acggttcttg
     1381 cgcgccagcg cccgtgccgc aatcaacgca tcgatctgcg cagcgtccag tgcacaacca
     1441 cccaactgtt gcagatgctg caccggatcg ctctgcagca ggccaagcac gcctcccaac
     1501 gcccgcagca gaccggcacg ctgtgatgaa cgcgatttat tcacctcggc cgccagttcg
     1561 aacagcactg acacagccag cgaggtgttc aggtcgtcgt ccatggcctg acggaaccgc
     1621 gctgcccatg gctcgtccca gtcgagttcg atcgtctctg gcggcacatc acgcaacgcg
     1681 gtgtacagac ggttcaacga ctggcgtgcg tcttccagat ggccatcggc gtagttgagc
     1741 gggctgcggt aatgcgcgcg cagaatgaac agtcgcagcg tttcaccgtc gaaacgctcc
     1801 agcacctcac ggatggtgaa gaaattgccc agcgatttgg acatcttctc gtcgtccacc
     1861 cgcacgaacc cgttgtgcac ccagtaattg gcaagcgtac agccatgcgc accctcggac
     1921 tgggctatct cgttctcgtg atgcgggaac agcagatcct gaccaccgcc atggatatcg
     1981 aaatgcggtg acagataatg ctcgctcatt gccgagcatt cgatgtgcca gcccgggcgc
     2041 cccatgcccc atggcgactc ccagcccggc tcgtccggcc gcgccgcctt ccacagcacg
     2101 aaatccagcg cgtcgcgttt gtacggatcg atgcccacgc gctcgcccgc acgcagttca
     2161 tcgagcgcct tgcccgacag ccggccataa ccgggaaagg catgcactga atagtacacg
     2221 tcaccattgg cggcgggata cgccagtccg cgctccacca accgcccgat caggtcgacc
     2281 atcgccggca caaagtgcgt ggcgcgcggc tcgtgggtcg gcgcaagcac gccgagccgc
     2341 tcggcatcct cgtgcagggc agtgatgaaa cgctcggtca gctcggcaat ggtctcgccg
     2401 ttgagggccg cacgtttaat gatcttgtca tcgatatcgg tgatgttgcg tacatattcg
     2461 acggagtagc cgcttgccag aagccagcgc accagcatgt cgaacaccac cagcacgcgc
     2521 gcgtgaccga cgtggcaata atcgtagacg gtgacgccgc acacatacat gcgcacctgg
     2581 cccgcaacaa gcggatgaaa tggctgtttt tccctgccca gggaattgtg caggatcaac
     2641 atgggggcaa cccgaaatcc atggtatgaa tcaagatgaa ttcttgctag aatcaacgct
     2701 ctgatcgaag tccagtgagt ataacatagt gacacgcccc ctcctttacg caattctgtt
     2761 cgcctgtgca atgctggcca gttctgtttc ccgggccgcg gacagcaagg aattccagaa
     2821 tatccagaca ctcttcaaac aaggtcagta cgcccaggcg ctcgaacgtg acaatgcctg
     2881 gatcgcgcat cacccgaaag acgcgcaggc ccgctttctc aaagggctga tcctgacgga
     2941 acagaacaag actgccgagg ccatcagcgt gttcacggcg ctcaccgaag acttccccga
     3001 actgcccgaa ccctacaaca acctcgcggt gctctacgcc tcccagggcg attacgacag
     3061 ggcacgcaac gccctcgaaa tggcgatcca cacgcatccg agttacgcta tcgccaatga
     3121 aaacctcggc gacatctatg ccaagatggc ttcgatcgct tacgacaagg cactgaagct
     3181 cgataccaag aacaataccg tgcataccaa gctggcgctg atcaaggaaa tctttgccga
     3241 acacccgctg gccagcgact gtactggcag cagcacagcg gcaacggcca aaggatcgtc
     3301 atcggcaatc gcaaccccgg ctgccacggc gaccggaaaa cccgcagcag acactcccat
     3361 gacgcctccg gccgcttccc aagcagcggc agcagcggcg gcaacgactc cggtcagcgc
     3421 accatccgct gcggatgagg ctgccattac acaggccctc cagagctggg cgcaagcatg
     3481 gtcgcatcag aacgccgacg cttatctggc cgcctacgcg ccccaattca aaactccgga
     3541 caacctgtcg cgcccgcagt gggaagccct gcgccgtgca cgaatcagca aacccaaaca
     3601 catcagcgta caactgtccg acctgcacat tgtgcaatcc gacaccgatc acgcaacagc
     3661 cggatttacc gaaacctatc actccaacct gatcgactcg gtcggccaca agacgctcaa
     3721 tctgacccga gttcacggca actggctgat tgtgcaggaa ctttctcgat aacgggctgg
     3781 acatgatccg catattcctt gttgcggcgc ttctcggctg gctggcaggc cgtccggcct
     3841 gggctgaacc gcccgcagat actgcaaccg ccgtcgcgac cgcaaccacg gctccgaccg
     3901 cattgacttc cgcatctgcc gaaatacctg cattggttcc cacacccagt gctgtgcctg
     3961 tgatgacatc cgcaaccacg gctccgaccg caacagtaac cacaacggct atcgcacctg
     4021 caagccagac ccagtcaagc cagacccaaa gtcacccgcg cacgctcgat cacgacgagt
     4081 cactgctggc aaccaccttc tctgcgctgc gcgagggcaa gctcgatgag gcccaacggg
     4141 atatcgacac cctgctgcat gcccagccgg attttcgcct ggcgcaactg atccggggcg
     4201 acctgctgct ggcccacgcc caggcgctgg accacatcgg caacacccgc caggactcac
     4261 ctgcgctgga agacttgcgc gccgaagcac gcgcacgcct cgaaagcgtg ctgcaaccgc
     4321 cacccgcagg catggtaccg gagcaggtat tgctgctggc agactcgatc cggcatatcg
     4381 tggtcgtgga cacggaaaaa tcacggctgt acctgtttgc caaccgcaat ggcatgcctg
     4441 tgtacctcgc agatttctac gtcaccatcg gacgcaacgg cagcggcaaa tcacatgaag
     4501 gcgacgaacg gacgccgctt ggcatctacc gcatcaccag ccggcttcac gacagcgcgt
     4561 tgttggccac ttatggcccg ctggcatttc cgctcgactt ccccaacgac tgggacaaac
     4621 acaagggcag cagtggccat ggcatctggc tgcatggtac cttcagcggc acgtacagcc
     4681 ggccgccgca tgccagcgat ggttgtgtcg tcctcaccaa taccgacctc gacagcctgg
     4741 cgaacttcat tgcgcccgac aatacaccgg tgctcatcac cgaccatatc cgctgggtca
     4801 cgccagatac ggtacttgcc gaacgccagc aactgatgca gcatgtcagc cagtggcaac
     4861 aagactggca aagcctcaat gtcgaacgct acctgggcaa ctattcaaaa gatttcaccg
     4921 cagatcaaac tggctttgcc gactgggcgg cacgcaagcg ccagatcgct cccggcaaac
     4981 gctggatcaa agtcgagctt tccaacctga gcctgtttga ataccctggc cagagcggtc
     5041 tctttcttgc ggacttccgc caggactacc agagcaacaa tctggtcaac aacatgcaca
     5101 agcggcaata ctggacgcag cagtccggcg agtggaaaat ccttgccgaa gacagcctgt
     5161 aacggccggc cgcccgcctt ctttttcaca cacccactga cggacaccat catcatgatc
     5221 aaactgcaca ccaactacgg caccatcacc ctgcgcctcg acagcgacaa ggcacccaag
     5281 accaccgcca actttctttc ctacgtcaac agcggctttt acgacaacac cattttccat
     5341 cgcgtcatcg atggtttcat gatccagggc ggcggcttcg agcccggcat gaagcagaaa
     5401 cccactgaag cgccgatcga aaacgaagcg gccaacggtc tgtccaaccg tcgctacacc
     5461 atcgccatgg cacgcacttc cgacccccac tccgccaccg cgcaattctt catcaacgtc
     5521 aaggacaatg gtttcctcga cttcagtgct cccaacgcgc aaggctatgg ctactgcgta
     5581 ttcggtgaag ttgtcgaagg ccaggatatc gtcgacctca tcaagggcgt caagaccggc
     5641 aatcgtgcag ggcaccagga cgtgccggtt gaggacgtga tcatccaacg cgctgaagtc
     5701 gtggcatgac cgtcatgccg gaggccggat tcagcctgct ggcctccgac ctgcacctca
     5761 gtccggcaca gccgcatgtc acacaccagt tcggcgagtt cctgcgccgt catgccggtg
     5821 gggcgcatgc gctgtacctg ctgggcgacc tgttcgaata ctggctgggg gatgacacgc
     5881 tcgatgcgcc gctggcgcag cagataatcg cctggctggc agagttgtcc actcggggaa
     5941 cacaggtgta cctcatgcat ggcaaccgcg attttctcat cggcacagag tttgcccgcc
     6001 gctgtggtgc caggctgctg gacgacccgt gcctgatcaa cctgcatggc acacccaccc
     6061 tgctcagcca cggcgacagt ctgtgcactg atgatctgcc ctaccaggcg ttccgccggc
     6121 aggtacgcca ccctgtctgg caggcacaat tccttgcgct acccgtagcc gaacgcattg
     6181 cacaagccga gacagcacgg cgccagagcg aagccgcgca acaggaaaaa tccgctgcca
     6241 tcatggacgt caaccttgac gccgtacgtg cactgctgcg cgcgcacggg tatccgcgcc
     6301 tgattcatgg acatacgcac cgtccggcgc gacacagctt cgaactggac aaccatgcat
     6361 gtgaacgctg ggtgttgccc gactggagcg acacccgcaa cggttggctt cgctgcgacg
     6421 ccgggggctg cagcttcgaa caactggcat agccagtgcc cgtgtccgga gcggacgcat
     6481 cagatcagcg cgccaagacc tcgtgccagg tcatcctgga tatcgcgcac cgactccagc
     6541 cccactgcca tacgcaacag gccgtcgccc acgccggcat cggcacgctg ctgcggcgtc
     6601 agccggccat gggtggtcgt cgccggatgg gtgatggtgg tcttggtgtc gccaagattg
     6661 gccgtaatgg agatcatccg cgtcgaatcg atcacatgcc atgctgcatc ccggccccca
     6721 cgcacctcga aggccaccat cgccccgccg ccagtctgct gacgcatggc cagcgcatgt
     6781 tgcggatgcg atggcaacca cgggtaccac acccgttcta ccgcaggatg ttcctccagc
     6841 catctggcca tggcgatacc gtgcgcggaa tgtgcatcca tgcgcaactt gagcgtctcc
     6901 agccctttga gcagcaccca ggcattgaac ggactcaggc tcggcccggc agtacgcagg
     6961 aaagtctgca ccgggttgag ccgctcatgc ccgcccagta ccgccccccc cagcacgcgc
     7021 ccctgaccat cgaggtattt ggtcgctgag tgcaccacga tgtccgcgcc cagttgcagc
     7081 ggtcgctgca aggctggcga gcagaagctg ttgtcgacca caagcagcgc gccggcgtca
     7141 tgcgccagtt cggacaaagc acgaatgtcg ctgatctcgg tgagcggatt ggaaggcgat
     7201 tcaacgaaca gcaggcgggt ttccgggcgc agcgccatgc gccagcgcgc aatatcggtc
     7261 gccggcacca gcgtgacctg cacaccgaag cgcggcatca cctgattgag caactgaata
     7321 gtcgaaccga acaacgactg cgaagccaca atgtggtcac ctgccgaaag cagccccatc
     7381 aacgtcgcca ggatggccga catgcctgaa gccgtggcca gacagcgctc ggcaccttcc
     7441 agtgccgcca gacgcgtttc aaacattgcc acgcccggat tgcctacacg cgagtagata
     7501 taacccggtt cttcgcccgc gaagcgcgct gccgcctcag cggcgctgcc gaacacgaaa
     7561 ctcgaagtca ggaacatcgc ttcgctgtgt tcgccaaatt cgctacgcac cgtacctgtg
     7621 cggatcgcca gggtctcgag atcgaaatcg ggctccatca ttcctcctgc aacggtttct
     7681 tgcccacgtt gacaccgagc tgcttgagct tgcggtacaa atgcgtgcgt tccacaccgg
     7741 cctgctcggc tgctcgcgtc atattaccct cggtctgtgc gatcagccat tcgaaatagc
     7801 ggcgctcgaa caggtcgcgc gcttcgcgca gcggcaggtt gagcggcacc gacaggcact
     7861 cgatcgcgtc atccgcgtcg acaccgctct ccggacccgg gaggtcgact tccatcggaa
     7921 ccggcgccac cggtcgtgca gctgccggcg atttcttcgg caacggatgc tgcaacccgg
     7981 ctgtcacgcc atcgagcagc ttgcgcaatg aaatcggttt ttccaggaag ccgaccgcac
     8041 cgatgcgcat ggcctccacc gccgtatcga tggtgccgtg tcccgacatc atgaccaccg
     8101 gcatggtcag gctgccattg ctgtcccact cgcgcagcag cgtgatgcca tcggtgtccg
     8161 gcatccagat atcgagcagc accagatcgg ggcgcatgcg ctcacgcacc agtcgtgcct
     8221 gcccggcgcc atcggccagg gtcacatcgt atccctcgtc catcaggatc tcggaaagca
     8281 gttcgcgaat gccgatttcg tcatccacca ccagtatgtc cttgcccatc atgccaccag
     8341 ctccatgtga tcaagcggtt ttgccaaacc gagtcgcacc cgcgccacag tcccgcccgc
     8401 cggaaggtta tccagcgtga tactgccgtg gtgttcctcg atgatcttct tcacgatcgc
     8461 cagccccagc cctgttccct tggccttggt cgtcgcgtag ggctcgaaca accggtcgcg
     8521 cacctgaccc gacaaccccg gcccgttgtc ctcgaccgcc agttcgactt ccttcccgct
     8581 gacggctgtc gtcatgcgaa tacgcggatg cacggtttca gtcagggcat cctgggcatt
     8641 ctgcagcaga ttatgaatca cttgtcgcag cagttttgca tctccagcca ccattggcaa
     8701 atccggatcg agcccgattt caatggttgc gtcatgcgcg tacaaatcaa tgacctcgcg
     8761 caccagggca ttcaggttca ccggcaccag ttgcgcactc ggcgtgcgcg catattcggc
     8821 aaaggcgtta accatctcct tcatcgccga cacctggttg acgatggttt gtatcgaacg
     8881 acgcaaggtc tccgcatcgc cgggtgacag tctgtcggcc agcttgtgct cgatgcgctc
     8941 ggccgacagc tggatcggcg tgagcgggtt cttgatctcg tgcgccagac gccgcgccac
     9001 ttcgccccag gcggcatcgc gctgagcacg caacaggtcg gtcacgtcat cgaacaccag
     9061 caagaaaccg ccatccatgc cgacaggcag tggaatgccg cgcaacagca aaatgcgtcg
     9121 tccgacgtcc gactccaggg cgaactgctg ctgccacacc gtgtcgccgt gcatgcggaa
     9181 ctggatcgac agtgtttcag ccagctcggc cagatgtgca aacgcaaccg gccattggtg
     9241 caacatctgc cccggcagga ttcccgacga cagaccaagc atctgctccg ccgccggatt
     9301 gagcccacgc acacgcaact ggctgtcaaa ggtgatgacg cccgaagtca ggctcgccag
     9361 caccgtctcc aggtaggcgt gcgacttttc gatctgtgcg cgccggtctt cggccacgtt
     9421 gcgcgtctcg gcaagctgcc gcgtcatgcg gttgaacgac tgcgtcagca tgcccagctc
     9481 gtcccggctc tgcaccgggt gcatctgact gtaatcgccc ttggcgacag ctcgcgttcc
     9541 ctgtgccagc gcccgcaacg gtgcggcaat ctgttcactg atcagaaacg ccagcgccac
     9601 ggtagtcagc agcgtcaaca ccatggtcag agtcaacgtc atgccataca gccgtttcag
     9661 gcccacgcgc gacagcgaca gtgcctggta atcctgatac gccgtctgca ccgcttcggt
     9721 ttcgcgtgcc agctggtcgg gtaccggctg caatacctgc agcacacgca tggtctccgt
     9781 cagcgacagc acattgacag gcagcaccac gcgcagatac atgccacgac cttccagcgc
     9841 ctcgacgcgc gagtacacat gttgctggcg cacctgaaac agcgtacgtc cgtccggtac
     9901 cgggggcggc gcccccatca tctccccccc tgccaccgac aacagacggc cgtgttcgtc
     9961 atacagcgag gcctcttgca ccgaatactg ctcgcgcaac tgcgccagtt cactcccctg
    10021 ccgcgccaga ggggcttcgg ccagccgctg cgccatcgat tcggccttgc cgttcagatc
    10081 atgctgcagg tcgtcgagtg ccgattggcc aaggtccagc ccgctttcga gcgcatgatc
    10141 cacacgcacg tcgaaccacg attcgatgct gcgcgacatg aattccaccg acacagcgta
    10201 gaccaccgtt ccgggcagca gcgccatcag accgaacacc agcagcaggc gcaccgtcag
    10261 cttggctccg aacagccccg cccgcatctt gcgacgcaac tgcaccatct ggtagagcac
    10321 cagtccgacc atcgccgtgg tcaacacccc ggtcgccccc agcagccagg gataatcgtg
    10381 tgcaaaaaaa ccggtgttcg cgctcgcgct cgacaggacg tagatcaacc ccgcaacggc
    10441 aacgaacccc agcagaacca ggtatttcac aaagtcatca tccacaccag cggcgcggat
    10501 tcaagatccc atctgccggt cgcgattgca ttgatctgca agggctttgg cagttcggaa
    10561 gtgtccagcc gcatgctcag cgtcgcacga taatcctgta tcttgctcag ccggtcgcgc
    10621 gcaatcaccg gccagccatc gatgcgtccc agtatggcca gggcatcgcc aagactgtcg
    10681 acgctgcgtg tacgcacgtc gacacccgac accacgtact ggcgcagtag tgtattgtag
    10741 gacaggcgcg tcacgcgcgt gatatgcgcc atctgttccg gaaaccagta ccagcgcggt
    10801 cgtgcgaaat cgaaatccgt gacgaaatac agcgacacgc cccgattgac tgcatcctcg
    10861 agcagcgacg tcagcgtgac atcaatctgc gcatacaggc aatatttgtc gtccacctga
    10921 cgcaactcgg cggaatggat ctggataccg cccgtcgcat gcgctgccgg cacgaacacc
    10981 catccgcaca gcagccagta gatggcgata ctaagtcttt gtaaccaggg catagaaaaa
    11041 cccgtccctc tcctcatccg gcaaccattg cacggaacat tgtccattat caagcggcaa
    11101 agtacgcgca tcggcatgac gagccaggaa gcgcgcagtc tgctgcacgt tttcgtccgg
    11161 aaacaccgag caggttgcgt ataacaattt ccctcccacc gccaggcacg gccaaagcgc
    11221 atccaggatc cgctcctgca actgcacaaa atggccgatg tcctgcggcc gacgcagcca
    11281 cttgatgtcc gggtgccgcc gcaccacacc ggatgccgaa cacggcgcat cgacaagaat
    11341 gcggtcgaac ggctgaccgt cccaccattc ctgcggccgc gccgcgtcac ctgtttggca
    11401 ccgcgcgctc agtcccagcc gctgcaggtt ttcgtccacc cgccgcaatc gctgtgcatc
    11461 ctgatcgagc gccagcagct cgacttgcgc cagttcgaga atatgtccgg tcttgccgcc
    11521 cggcgcagcg caggcatcga gcacgcgcat gccgtcctga gcatcgagcc agtacgccgc
    11581 ctgttgcgcc gccacatcct gcaccgacac ttctccggca tggaacccgg gcaattcgcc
    11641 taccggaacc ggccgcacca gacgaatggc agtgtcaccc accacgctgg cctcgatgcc
    11701 cgcatcgcac aaccgttgca catactgcag cacactgatt ttgcgcgtat tgacccgcaa
    11761 tgtcatcggg ggatgccgat ttcctgcttc aagtatacct tgccattgtt gcggaaatgc
    11821 aacagtcagc cggtccaccc accagcgcgg ataggcaaga cgtgacaccg ggtccaccga
    11881 caatcgagtc atgcccggcg cgccatcctg caaccaaccc tgccgcagca cgttgcgcaa
    11941 caccgcattg gtcagccctc ttgcccagcg cttcaggctc gcggccgcct cgaccgcctc
    12001 gctgacggta cggtatgccg cctcggggtg caactgtagt tcgcgcaatg ccacaagcag
    12061 caggatggcg acctggcgat cctgcagctg acggtccagc gcggcatcgc gtaccgcttc
    12121 gagccagcca tattcgcgca ggcaggcaaa gcacatgtct tgcacccgcg cccgacccgc
    12181 ttcgctttcg cgcgccagca gccggtccag attgtgtttg ccatgcacca cgctgtcgat
    12241 gacccgtgtg gcgcgcaata aaagctggcg caacggtggt tgcgcgacat catccctgtt
    12301 gatcatacgt gatagcccgc cagtcccgat gtcatccaat gcagcaatcc gatcaaccca
    12361 aaccccgcca ccagcatacc cgccagcctg cgcagccaga ccgactggcg ccatgcctgc
    12421 agatagcgcg caaaccagcc catggccaac agattgggca acgttcctgt accgaatgcc
    12481 agcatcacca gtgccccgtg cacggcactg ccgcttgcca gcgccgacac cagcaccgaa
    12541 tacaccagtc cgcacggcaa ccagccccag accagcccgg ccagcacggc ctgcccggga
    12601 tgccgcaccg gcagcagatg gcggacgtac ggctgcagac gcagccacag tccgcctcca
    12661 agccgttcga tcccgagcac agcacgcgac cagccggcca ggtataaccc cagcaacagc
    12721 agcatgaagc tggccagcgc atacagcacc cgcgaaaccg gcagcacatg atcgagcagc
    12781 aatgcgctgc tgccgacggc accggccagc gccccggcca gcgcatagct gaccagccgc
    12841 ccggcattat aaccgagcag aataccccac ccggcccgac cgccaggtgc agacagcgat
    12901 aatgcgctga cgataccgcc gcacatgccc agacaatgca caccgcccag ccagcccacc
    12961 agcatcatcg acaacaggct atattcgatc atgacccggc cctttcggca cgacccacca
    13021 accagagcag tgccagcacg ggcagcccca gcatcgcggt gaatacaaaa aatccagcat
    13081 acccccacgc attgaccagg tcaccggaaa acccggcaag gaatttcggc aacagcagca
    13141 tcaccgaact cagcaccgca tattgcgatg cggtgtacgc ctgattcgtc aggctggaca
    13201 accaggcgat gaacgcgctg gaaccaatgc cggccatcag gttatcggcc gagatcaccc
    13261 acaccagcgc atgcacatcg tggccccggg ccgcgagcca gacataaaac aggttgccca
    13321 tcgaggacag caccacgccc agccagagta tcctgcgcac accgaaacgg gccaccagca
    13381 ccccgcccag cgcggcgcct gccagactca tcaacacacc atagatcttc gacaccatgg
    13441 ccacttcggc tttggtgtaa cccagatcca cataaaacac gttggccatc acaccaagta
    13501 cgacatccgg aatgcgataa caggcgatca gcgccagcat cagcagtgcg cgcttacggt
    13561 agcgccgcat gaaatcggag aatggcgcgg ccaccgtgcc ggacaaccag cgcatcgccg
    13621 ccggcagtcc cgccgcacgc gcaacctccg gagtccgctc tcgcgccacc tcatgcatcc
    13681 acaacgtggc aaacataccg accatcatca gcgccgccat gaccagataa gcgttgcgcc
    13741 acgcagcata tacataaccc tgtgcagcgc ccgccgtcca cgcggccagc cacagcacgc
    13801 ccgctccggc aacaatcatg ccgatgcggt agccggcctg gtacgccgcg gccatcaccc
    13861 cctgccaatc ggcttcgacc gcttcgatgc gccaggcgtc gagcgcaatg tcctgggtcg
    13921 cggcagacca tgcgatcagc ccggcccaca tcgccgtcaa cgccagaccg ttgcgcggat
    13981 cgtgcaacgc cagacctgtc agaccggccg ccacgacaca ctgcgccagc agcagccacg
    14041 atcgacgcca acccagccag cgcgtcagcc cgggcagtgg caaccggtct accagcggtg
    14101 cccatagcca tttgaaaccc caggccagcg ccacccaggc catgtgcccg accgtggagc
    14161 ggttcacgcc cgcttcgcgc agccagaacg acaacgtgcc cagcaccagc aacaacggca
    14221 gcccggacga gaacccgagc agcagcatgc cacgcaccct gcggtgggta tagacagaaa
    14281 atgcactcag ccagccacgc acgcccgact cacccctcag gccgtgacat catcataatc
    14341 gacgatcagc ggcgcgtggt ctgaaaaacg ttgcgccttg tacaccgtcg ccacccgggc
    14401 gcgcgctgca attgccagcg ttgccagctg gtaatcaatg cgccaaccga catttttcgc
    14461 ccaggcctga ccgcgatgcg accaccaggt atagccctct tccgtagcct gcggatacag
    14521 ttgccggtat acatcacgcc agccgagtac gtcgagcaca cgtcccatcc acgcacgttc
    14581 ttcaggcagg aagccggaat ttttctgatt gcctttccag ttcttcagat caatttcttt
    14641 gtgggcgatg ttccagtcac cacacagcac gcgctcgcga ccgtcttgcg ccagcgcagc
    14701 gagtcgggga tagaattcgt ccatgaacgc gaacttggcc gcctgacggt gctcgccact
    14761 cgaacccgaa ggcaggtaga gcgacgcgac tgccagattg ccgtgacgca cctcgacata
    14821 acgcccctcg gcatcgatgt cggccacgcc aagaccggta atcacctggt cgggtgcgtg
    14881 ccgggtgtaa atacccacac cgctgtaacc ttttttctgc gcataatgaa aatggccggt
    14941 atagccgtcg ggggcgagca tctgtgcggt catgtcggca gcctgcgcct tgagttcctg
    15001 cacgcagaca aagtcggcgt cctgcaccgc cagccaatcg aaaaagcctt tacccgctgc
    15061 ggagcggata ccgttgaggt taaccgttat aatgcgcatg tttccttatc cattgttttg
    15121 ccaccatgcc cgacttccgc cagcaatttc tgcagttcgc catcgatcgc cgcgtgctcc
    15181 gtttcggcga attcaagacc aaagcaggcc gcctgagccc gtatttcttc aatgccggcc
    15241 tgttcaatga cggcctcgcc ctcaaacagc tcggagaatt ctacgccaaa gccatcctct
    15301 ccgccggtat cgacttcgac gtgctgtttg gccccgccta caagggcatc ccgctcgccg
    15361 ctgccactgc gatcgcgctc gctgcgtccg gacgcaacgt gccatggtgc ttcaaccgca
    15421 aggaagccaa ggatcacgga gaaggggggc acctgatcgg tgccgagctg cgtggccgga
    15481 tactgatcat cgacgacgtc atctctgccg ggacatcggt gcgcgaatca gtggacatca
    15541 tccgccacca cggcgccgaa ccggccggag tgatcattgc gctcgaccgc atggaacgcg
    15601 gccaggggac gctgtcagcc gcccaggaag tcgaacgctc gcacggcatc ccggtgttct
    15661 ccattgccag tctcgacgac ctgctggact atttgcaaac gggcacggac atgaaacaat
    15721 atgaacaggc aattgttcag tatcgatcga tttacggagc cgttcctcca tgatgcgaac
    15781 ccgcatgatc tgtctgcaca ccggcctggt gctggtgctc actgcgctgt cctgcgttgc
    15841 ccgtgccgac gccatctaca agtggcgcga cgccgacggc gtggtacatt atggcaacac
    15901 cctgccggca aaggatgccg gtctggcggt gacgaccatg gacaagcacg gtctcgtcgt
    15961 caaacagacc cccgcagcgc ccaccgagca gcagcgccag gccatgcagg ccgaggccgc
    16021 ccgccaggcc cgcctgcagg cggcacaaat cgaacagcag cgccaggata ccgctctgct
    16081 caacacctac acttcaccct ccgaaatcga cctggcgcgc gaccacaacc tcaaactggt
    16141 gcaactcgtc atcaacggca tccagtcccg tctgggtctg ctctatgaca aacgccagca
    16201 actgctgcgc gagtcaggcg gcaatattcc caccagtggc aacacagcca tcgagttcaa
    16261 tgccaacgaa caacacatcc gtgacctgac gacgcagttg caacaaaaaa atcgtgaact
    16321 cgcagccacc cgcagcaaat acgatgcgga aaaagcccgt ttcatcgaac tgaccagcaa
    16381 aagccgcccg tctcaggtcc agtagccgca gctgcggcga ctgaaacccg ggcggctgcg
    16441 gcacaaccgc agtacgctcg cctcacgcct tgagtttttc cagcaagatc tcgttgagct
    16501 gctgcggatt ggccttgcct ttgctcgcct tcatcgcctg cccgacgagg aagccaagca
    16561 tcttttcctt gccggcacgg tattcggcca cctgtgacgg acaggccgcc agcacttcat
    16621 ccaccagcac ggcgatcgca gcgctgtccg acacctggcg cagtccacgc gcatcgatga
    16681 tcatgtcgac atcaccttcc tgctgccata acgcgtcgaa cacctgtttg gccagcttgc
    16741 cggacaacgt gccatcctga acccgccgga cgagttgccc caactgctct gccgtcaccg
    16801 gacaggcgga aaattcgata tcgtaccggt tcagcgcacc cagcagttca cccatcatcc
    16861 agttggcgca ctgttttgcc gcaccggagg aaaccgccac gcaacgctca aaataatccg
    16921 ccatctggcg acccgaagtg agaatgccgg catcgtacgc cggcaaaccg tactgctgca
    16981 cgaagcggtc acgcattgtc gccggcaact cgggcatatc ggcctgcacg cgcgctatcc
    17041 actcgggcga aatcaccacc ggcaacaagt ccggatcggg gaaatagcga taatcgaaag
    17101 cctcttcctt ggtacgcatg gtacgggttt catcgcgatc ggagtcgtac agccgcgttt
    17161 cctgacgcac cgtacctccc tcctcgatca gctcgatctg gcgttgtgcc tcgaactcga
    17221 ccgcgcgctc gaggaaacgg aacgaattga gattcttgat ctcgcaacgc gtgcccaact
    17281 cgctactacc catcggtcgc accgatacgt tggcatcaca gcggaagctg ccttcctgca
    17341 tgttgccgtc acagataccc agccagacca ccagcgcatg cagcgtccgc gcataggcca
    17401 ccgcctcggc agcgccacgc aagtcgggtt cggagacgat ctccagcagc ggcatgccgg
    17461 cccggttcag gtcgatgcca gtcatgccat gaaaatcgcc atgcatcgat ttgcccgcat
    17521 cttcttccag gtgagcacgc gtaatacgta tggttttctc accgtgcggc agcaggatgg
    17581 tcagggtgcc cccttgcacc accggttcgt ccatctggct gatctggtaa cccttgggca
    17641 ggtcggggta aaagtaattc ttgcgcgcaa acaccgagcg cgggttgatc ctggcaccga
    17701 tcgccagacc gaaacggatg gcccgttcca cggcaccgcg gttgagcacg ggcagcacgc
    17761 caggcaaggc gatgtcgact gtgcttgcct gc
//
LOCUS       DAIANL010000151        23543 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1625837, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000151 DAIANL010000000
VERSION     DAIANL010000151.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 23543)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23543)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23543
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625837"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4931 a         7769 c         6559 g         4284 t
ORIGIN      
        1 gccgctcgaa gaactgccgc tcatcgtggt catcatcgac gaactggccg acctgatgat
       61 ggttgccggc aaagcggtgg agcaactcat cgcccggctg gcacaaaaag cccgcgcttc
      121 gggcattcac ctcatcctgg ccacccagcg cccctcagtg gatgtcatca ccggcctcat
      181 caaggccaat gtgcccacgc gggtggcatt ccaggtttcc agccgcatcg actcgcgcac
      241 catcctcgac cagatgggcg ccgagaccct gctcggccag ggcgacatgc tctacctgcc
      301 gcccggcacc ggttatccgc aacgcgtgca cggtgccttc gtggccgacc atgaagtgca
      361 caaggtggtc gaatacctca aacgactggg cgaaccacac tacctgcccg acatcctcga
      421 aacacgcgcc agcgacgatg gcgccgaacc cggggaaggc gatgcggaaa ccgacccgct
      481 ctacgacgag gccgtcagtc tggtcgtgcg cacgcgccgc gcttcgatat ccgcggtaca
      541 gcgccagctg cgcattggtt ataaccgtgc cgcccgtatg gtcgaagcca tggaacgggc
      601 tggcatcgtc agcgctcccc aacccaacgg caaccgggag gtcatcctcc cacgccagga
      661 atgaatccgt gaaatccttg ctcaccctgc tgcttgtcac ctgttccctc accgcccacg
      721 ccggcggcat cgatgccctg cacgccttcg tggcgcacag cagaactgcg cgcgcgcagt
      781 tcagccagac ggtctacgac agccacggcg ccgtgcgcca gcgctcggac ggcaccatgg
      841 agttctcccg gccgggacgg ttccgctggg tctaccgcgc gccatatgaa caggtcatca
      901 ttggcgacgg caaaaaaatc tggctatacg acgccgatct ggcgcaactg accatccgca
      961 acctggacaa ggcactcggc agcagcccgg ccgcactgct ggccggtgac aatgccatcg
     1021 aacgctattt cacgctggca gacgacggca gccgcaatgg cctcgactgg ctgctcgcca
     1081 cgccacgcga agccgactcg agcttccgca gcatccgcat gggtttcaat ggcgacgaac
     1141 tggtagcgct cgatatcacc gacaacttcg gtgggcgcac ggaactgcgt ctgcaacaca
     1201 tcgaacgcaa tccgacactg cctgcaagcg attttcgttt cacaccgcca ccgggcacgg
     1261 acgtgctctc cgacacaccg tgacccgcaa cctgttcccg cccagcgatc cacctgcacc
     1321 gcttgccgaa cggctacgcc cgcatgcgct cgacgaggtg gtgggccagc gacagctgct
     1381 cgatcccggc aaaccgttgc gcgccgcctt tgacagcagg catgcacatt ccatgatcct
     1441 gtggggacca cctggcgtcg gcaagaccac gctggcacgt ttgatggctc atgccttcga
     1501 ctgcgagttc attgcacttt ccgctgtcat ggccggcgtc aaggacattc gcgccgccat
     1561 cgacagcgcc gaacacgccc gtgaacaaca tgggcgacag accttgctgt tcatcgacga
     1621 aatccaccgc ttcaacaagg cacaacagga tgcgctactg ccgtatatcg aatccggact
     1681 ggtgatcttt gtcggcgcca ctacagaaaa cccttcgttc gaggtcaact cggccctgtt
     1741 gtcccgcgct caggtctatg tgctcaaggc gcttgacgat gccgacctgg ccggcctgtt
     1801 cgaccgcgcc cgcaaccgcg aatacccgca actgcatttc gagcaggcag ccgtcaccag
     1861 cctgatccgc caggcagacg gcgacgcgcg ccgtctgctc aacctgcttg aacaggtcgc
     1921 ctgggccgca caaaccgcag gcgtcaccca cgtcgatgca gcattcgtgg ccgagattgc
     1981 cagccatggc gcacgccgct tcgacaaggg cggtgatgtg ttctacgatc agatttccgc
     2041 gctgcacaaa tcggtacgcg gttcgcaccc cgatgccgca ctgtactggc taacgcgcat
     2101 gctcgacggc ggcgccgatc cgcactacct ggcacggcgc atcatccgca tggcatggga
     2161 agacatcggt ctcgccgatc cgcgagccat ccagatcgcc aacgatgcgg ccgccaccta
     2221 tgaccggctg ggcagtccgg aaggagaact tgcactgggt caagccgtga tctacatggc
     2281 tgtcgcggcc aaaagcaatg cgggctacct ggcgtacaac caggcaagag ccttcgtgaa
     2341 gcaggatcag agccgtgagg ttccgataca cctgcgcaat gcccccaccc ggctgatgaa
     2401 agaactgggc tatggacacg aatatcgcta tgcccatgat gaacccaatg cctacgcagc
     2461 cggcgaaacc tacatgccgg atgacatgca ggaaccggcc tggtatcaac cggtgccacg
     2521 tggcatggag atcaggattg gcgaaaaact gacgctgctg aaacagctgg atgaagatgc
     2581 caggaagaaa aaatgaagga cattctccag cggattatcc agatttcagc ctcgccacgg
     2641 caaaagaaca ccgggtaatc cgcatcagga ttgatgcgca atacgctcct gaatcagctc
     2701 acacgatatt tttcaatatc ttcaccggca gcctggacat ccagcaccca ttggggtttt
     2761 ctgccacgag ccgctcccga ccagattttt tcaccattgc ggtacgtcac tttcacttcc
     2821 ttgcctgcgg ctgttttcct tgccgttttt tttgatccgg aaccaaaacc cagttgttcc
     2881 gcagtcaagc cgtattgact gattttgcgt ttcatgtcgg ccaggacagc atcgatctcg
     2941 gcttgtttga tcgcttctgc ctgctgctga agttttttga tctgctcctg caattcggca
     3001 taggttggca tgtgttttcc cctttcagat aaattattac cacaggtcga atcttacttc
     3061 attcttggac attttttaag tacaacgctt catttcatag aaaattgata aaagaatcgc
     3121 attcatttaa aaatatttaa atgttattta atggtgcggt gattaaatcc gcgtgatcgc
     3181 gacgtcgatc tggagggatg gattgcaagg ctgttttaaa aaatccaggc ggacattgca
     3241 gcactcaatc aatgcggcat gaccgtcacc ccgacaagcc cacaatcaga aatcgtcgtc
     3301 aatatccgga acgaatactt cagtggcata atatgaaacg aaaaagcgcg ggaatgacac
     3361 ggattcatca tcggaatggc ggcaacggcc gcgtgcaacg actcaggctc cgcatcggcc
     3421 tgtttgggga aacacaccat ggaaaccaag gcacactggg aacacatcta ttccagccgg
     3481 atggtcaccg aagtcagctg gtttcaggaa cacgcggccc tgtcgctgca atgcatccgc
     3541 gaggccaata tcctgcccgg acaagccatc atcgatgtgg gtggcggcgc ctcgacgctg
     3601 gccgacgatc tggtcgacgc gggacaccgg cagataaccg ttctggacct gtctgccgct
     3661 gcgctggcag cagcacagca acgcatgggt gaaaacgcgg gtcaggtacg ctggatggaa
     3721 gccgatattc tggatgtcga actggaaagg catgcctatg atgtctggca tgaccgggcc
     3781 gtgtttcatt tcctcacttc gccgcaagcg cgcgagcgct acgtacagac cgttctgcat
     3841 gccgtcaaac cgggcggact ggtgattgtg gcgacatttg ccgaagatgg cccggaacag
     3901 tgcagcggac tgcccgtggt acgctacagc gccggcagtc tgcacggcac atttggcgaa
     3961 ccgttcatcc tgcttggcca caaacgggaa tcccaccgga caccaggcgg caaggaacag
     4021 cgcttcatct attgcttctg ccgcaaaacg acctcctgac tggcgacagt gcactgcgcc
     4081 gcatagccgg cctcctgccg gatcgccgtc gccgcgtcgg ttgccccgcc tgacggcgga
     4141 gcagactgcc attacttacc gaacaccatg acgccgtcac ggtaatcgac gctgatgatg
     4201 tccttggcca tgaaacgtcc ggcaaggatt tcccgcgcca gcggattttc cagttgcgac
     4261 tggatggcgc gcttgagcgg ccgtgcgccg aacaccggat cgaaaccggc ccgggccagt
     4321 tcgtcgagtg cggcatcact gacctccaga cccatgtcca tcgcagccag ccgccgctgc
     4381 agcgcttcca gctgcagtgc tgcgatcgaa tggatgtggg cgctgccgag cgcgtggaac
     4441 accaccactt cgtcaatgcg gttgatgaac tcggggcgaa aatagctttt cacctcgctc
     4501 atcacggcgt ccttgaccgc agcataagga tcgccgctca tctgctggat catctgcgag
     4561 cccaggttgg acgtcatcac gatcaccgtg ttcttgaaat cgaccgtgcg cccctgtcca
     4621 tcggtcaggc gaccatcgtc gagcacctgc agcagcacgt tgaacacgtc cggatgcgcc
     4681 ttttccacct cgtccagcag aatcacgctg tatggcttgc ggcgtacggc ctcggtgagc
     4741 gtcccccctt cctcgtagcc gacatagccg ggaggggcac caatcagccg cgacaccgaa
     4801 tgcttctcca tgaattcgct catgtcgatg cgcaccaggt gttcttcgct gtcgaacaga
     4861 aacccggcca gtgacttgca caactcggtc ttgcccacgc cggtcggccc aagaaacaga
     4921 aacgatccat agggacggtt gggatcggcc aacccggaac gcgaacggcg gatcgcatcc
     4981 gacaccagcc gcacggcttc atcctgcccg acaacccgct tgtgcaacac gtcttccata
     5041 tgcagcagct tgtcgcgttc accttccatc atcttggaca ccggaatacc cgtggcgcgc
     5101 gacaccactt ccgcgatttc ctcggcgccc acctgggtgc gcagcaaccg cttgggcgta
     5161 gcctgttcgc tactctcggc ctgctgcgcc tgggtcagct gcgattccag ctgcggaatg
     5221 cggccatact ggatctccga catcttctgc cagttgccct gacggcgcgc gtcttccatt
     5281 tccacgcgcg cgtgatcgag ttgctccttg atgtgctgcg tgccggccac catggccttc
     5341 tcgcctttcc acaccgtttc cagatcggcg tattcgcgtt ccaggcggcc aatttcctct
     5401 tccagcagcg ccagacgctt gagcgacgcc tcgtcctttt cctttttcac cgcctcgcgt
     5461 tcgatcttga gctggatcag gcgacgatcg agcttgtcca tcgactccgg cttggagtcg
     5521 atttccatgc gcacgcgcga ggcggcctcg tcgatcaggt cgattgcctt gtccggcagg
     5581 aaacggtcgg taatgtagcg gtgactcaat tctgccgcag ccacgatggc cggatcggtg
     5641 atgtccacgc cgtggtgcag ctcgtatttc tcctgcaggc cgcgcaggat cgccacggtg
     5701 tcttccacac tcggctcatc gacctggacc ttctggaaac ggcgttcgag cgccgcatcc
     5761 ttttcgatgt atttgcggta ttcatcgagc gtggtggcac cgatgcaatg cagttcaccc
     5821 cgggccagcg ccggcttgag catgttgccg gcatccatgg cgccttcggc cttgcctgcg
     5881 ccgaccatgg tatgcagttc atcgatgaac agaatgatgc gaccttcgtc ctgagccact
     5941 tccttgagca ccgacttcag gcgttcctcg aattcgccgc gatatttggc acctgcaagc
     6001 agcccggcca tgtcgagcac cagcacgcgc ttgtccttga gcgactccgg cacctcgccg
     6061 ttgacgatgc gctgggccaa tccttcgaca atggcggttt ttcccacgcc gggttcaccg
     6121 atcagcaccg gattgttctt ggtacgccgc tgcagtacct gcaccgtacg gcggatttcg
     6181 tcgtcgcgac cgatgaccgg atcaagcttg cccgaccggg cacgatcggt cagatcgagt
     6241 gtgtatttgg tcagtgcctc gcgttgccct tcggcttcgg cgccatgcac accctggccg
     6301 ccacgcatct gcgtcaccgc ggcatcgagc gcctggcggg tgatgccgtg ctgtttgagc
     6361 aatcgtccgg tttcgccctt gtcatcgcag gcagcaagca gatacagctc actggcgatg
     6421 aactggtcac cgcgcttctg cgcgtctttg tccgtcatgt tgagcaggtt gttcagatcg
     6481 cgcgaaattt gcacttcgcc gccatgacct tccactttcg gcagacgttc gatggcgcct
     6541 tgcagcgccg tgcgcaacgc acgcgcattg ccaccggcac gcgcaatcag cgaagcgctg
     6601 ccgccttcct ggttgtccag cagcgcagcc agtacatgtt gcggctcgat gtactggttg
     6661 tcctgtccaa cggccaggct ttgcgcatcg gccagcgcct gctggaattg tgtggtcagc
     6721 ttgtcgattc tcatgacggt ccccgaaaat gatctgagcg gtatatgagg caatcattgc
     6781 cgatttcaac acccacccgg ccggggggac tcagtcatgt accatcaccc aatcttccca
     6841 caagtgttcg acctggcgca tggcttcaag gatacggccg ggcgcaaccg acacccccac
     6901 actgtcatcg gtgaagaaga tcccggcccg cacacctgcg acgtcgagac gcggcaacag
     6961 gttcatcagc agatcatgcc gatcaaacgc tgcggcgcga aaatcccgcc atcgcccctg
     7021 acctgcatgg tcgcggaaat gaagcgccag ttccgccgcc ggccacatca gaaacgcagg
     7081 ctgtccatct gaagccgccg gcagacatgc ccagcgatcg tcgcattcca gcccccatat
     7141 gcggcatttc cataccgctt ccctgatgaa atgctgaaac cggcgccccg gatccatctg
     7201 cagcaggtgc tgcaactgct gccggttcaa tgcctgcgtc atctgatgcc acggtacaaa
     7261 tgcaaattcc atgattcccc cagcgtacga acgacacaac tgtcccgtct ctcgacccga
     7321 ccgcctgcga tccattgttg cagtcacaga tgacacgggc aagacaacat gcattatatt
     7381 tattaacaat tgacaaaaca atccgccgat ggaacaatgc gcgcatgaat gtggacaagg
     7441 aacatgatat gaaaggatta acgctacgcg tactgctgtt ggcagcaatt gtttcatggc
     7501 caccacattc cgcctgcgcc gcgcagtccg gcgcccccgg atcctcgcaa accggtgcct
     7561 atgcgtccgg cgaccatgcg accaaccggt acagtcgggc cgccatcgac acgcgccgca
     7621 actggttcta ccagcagcag gcaaaatacg cctcgacgcc gggcttcgat ccgcgccgtc
     7681 ccggctacag cgaccggggc accgccgacc ggcccggcag gaatccgctg tacaaccgca
     7741 aatacgatca cgaagcccgc ctgcgcgcac tgggcgatga tcccaaagtc gcttcggccg
     7801 accgcggctg gattcaccag gagatccgtc gcgtggacct gaaccgcagc cggccccggt
     7861 acctgcgctc cggttcggac accctgcgca atcccccggg caccgacctc gcccacgcac
     7921 atggccagga aaaccacaag ggttacggct ttcactatgc gcaaatccag aactcggacc
     7981 tgcatcgcct gcaacatacc ggctatctgg accacacctg gcaatacgaa cgcgatcatg
     8041 acaatccagc ggtcggcggc gactggtggg ggcaacgcaa cagggaacgt gttcctccgg
     8101 aataccggcc aggcgcgcca gcggcaatga acccggtgac aagattcgcc aactgacacc
     8161 atccatctcg aacccgggag aagtgaacgc atgaaaacac agctccgcca gatggccgcc
     8221 tgccttgccg ccagcgtcat gctggccagc accgcagtcc atgcgggcac cggctattct
     8281 gcagggccag gcttcggcaa cccctatggc cagggccgcc cctaccagaa cgcctacaac
     8341 ccggcgcccg gcaaccggaa tgcattacag ggccagtact tcccgccatc gcagtcaggc
     8401 agcgcccgga ccggccaacg gccgtccggc gcttcggcca gcaggcaagg cgattcagcg
     8461 aacggacgca gttcggcccg tgcaacccgt accgtccagc agatcgcccg cgactacccg
     8521 cccagcaact cgcccaatcc gcaaaaacgc gcccgcgaag cgcaactgcg cgcgctggca
     8581 gaaaaacgcg gtttgccggc gaaacaacgc aaatggatcg tgaaacgcta cacacacatc
     8641 gataaccggc gtaccgaaat ccgtcaggct gaagcacgct accaggcaag atccgcaaga
     8701 caaagccgca gcgacagcac agcttcatcc cgccccctgc ccacggccta tcgttgagag
     8761 gtaatgcaat gggactatcc gccagaccgc tgcacgaact gcatgacccg gccaccacgc
     8821 ccgatcacca gtacatgggg gcgccccgac cgcacgtcag tatgcgaccc gaccactgga
     8881 cgcacgccat gcacaaattg gtcgcatttc tgctcaccgc cctggtcctg ctgaccttcc
     8941 cagcccaggc cggaactggc tatcttccgg gcagcgctgg ccagacacca cctgcccgga
     9001 tgtacgtcgc aggccagatc catgcaacga gcgcgccgcg tcagcgcccc cggaacagca
     9061 atccatccgg ctggttcaac cggcaacagc aggctcgtac ggctgctagc gggccgttcg
     9121 cacaacaacg gttcggtcgg gtcggccagc atgccgttcc ggagcacgac gcagccttcg
     9181 agcgcgaagc gcaactgcgt gccctgacca aagacccgcg ccttggccgc agcgaccgcg
     9241 gctggctgca tcaggaaatc catcgcgtcg agcgcaaccg cgagcgcatg cccgtcgcaa
     9301 ccggatcacc ggaagacacc gtgcgccccc cattcggcaa gacactggtt caacatcgca
     9361 gtacacggta ccctggaacc gccctgcagg atatcgccct ggaacatctg cgcacgtccc
     9421 acccggcacc ggcatcatcg gggcgcagcc caggtcaggc caccggatcc agtacgtatc
     9481 gttgaacacg gcgaaccacg cgcagatgtc atgcgcaggg ttgattccct ggcagaaaat
     9541 tttcatcccg accacaggag cccacatgga actggtctat gaaccctcgc gcagccaacg
     9601 cccccatacc caccccaggc aactgctggc gttgagcgcc ctgccaccgc gacagcgttt
     9661 cgagcatttt ctgcatgagg cactggcacg ccgggaagtg tggggactat gccgcggcaa
     9721 acactggttg ttgatccgca cggtggaaaa cggactcgcg ctaccgcttt ggtccgcctg
     9781 ggaacacagc gaagactgga acgcgcacca ccaccagatc tgggctcatg actacgcacc
     9841 ccgcgccatc cgtctcggcg agctcaccca cgaactgata ccgctgttgc gcgccgaaca
     9901 actggccatc ggctgttttt tcgtaccgga aaacaatgga ctcaccatga gtcccgatgc
     9961 cttgtgcagt accctgctga ccatgctgcg ttcaatggat acgctcgcca gccagtgacc
    10021 tccggggcaa tggaaccagc acacgccggt gtgcttgcat ccggtctggg ggacaacagg
    10081 cgatgactcc gaccggagcc ggctttcgcc atcagggcgg tcgcagccgg acgggacttt
    10141 cgttcgccat cggagcagtc gcagccggac gggactttcg ttcgccatcg gagcggtcgc
    10201 agccggacgg gactttcgtt ccgcctgtgc cctagctctg accaccggca tgcggcgaag
    10261 caccagctgg ctggtccttg gccggcaagg cgttccacca gccgccaccc agcgcctggt
    10321 acagcgctgc ggtatcaccg agccgctggc tgcgcgcctg aatcagggca atatgcgcct
    10381 gcagccagct ctgctcggcc gagagcagca ccagcaggtt gacatagccg acagtgttct
    10441 gtttacgcgt caggtcaagc gtgactctgg ccgcctgctc ggcccgcacg gccgccgcca
    10501 tcccgtcagc atcggcatgc aacgcgtgca gggtgtctgc aacattctgc aatgccgtga
    10561 gcaccgtttg ccgatactgc gctgccgcct gcaccaatgc ctgatccgct gcccgctccc
    10621 gatgcagcag ggtgttgcca tcaaaaaccg gctgggtgac gttacccacc agcgcccaga
    10681 atggaccgcc actctggaac atctggttga acaccgaggc ttcgcccccg accgcgccca
    10741 caatggaaaa ctgcggcaac cgcgctgcaa cggcgacccc gacattggcg ctggcggcat
    10801 gcatctgttc ttcggcggca agcacatccg gacgctggcg caccagctgc gacggcaggc
    10861 tctcgggcaa ttgctgcggc aggcggaaat catccatgga aaaatgctcg acgtcctgat
    10921 cgggcgtgtt gccagccagc acgcgcaaca ggtcgcgcgt ttgttgcaac tggcgttcga
    10981 gcggcggcag ctgctgatgc gccaccgcca gtgtgctctc ctgcgcggcg acgtcgatgc
    11041 gcatggcata accgctggac agttgccgct gcaggatgcc gagagcacgc tggctatcgg
    11101 caatcatcgc tttcaccgcg tcaatctgcg cacgcaaggc agcctcctgc aaggccgcgg
    11161 ccacgatgtt cgaggccagc gtcacccgcg cagcctcgag ctgatagcgc tgcatgtcgg
    11221 cctgagcccg aagcgattcc acctgacggc ggttggcgcc gaagacatcc ggcgtatagc
    11281 ccactgtcaa ctgtgccgta tggaaggtat aaatgaccgg accattgtac ggcgccggtc
    11341 cagcgggatt ctggtaggtc tggatgatgg tgccattgcc ctggattccg ggcgagtttc
    11401 cacccatatt gccggccagt tgctgccgcg agggcgaata gctcagaccc accgtcgggt
    11461 aaaaataacc ctgctgggca tacacatatt cctgcgcctg cttcagtgcc gcctgcgccg
    11521 aagcgatgcc cggattggcc tgcatcgcgc gccggatcag cgcatccagg cgagcagaat
    11581 gaaacagcgt ccaccagtcg gcaggaatgt cacgctgtgg gtcgaaatgc tgtgccgcac
    11641 ccaacgggtc ggcggccgca gcggtctgcg atggcatggc ctgcggcgcg taaccctgtg
    11701 cggcaggcgg ggccggcggc ctgaaatccg gcccgacggc gcaaccggtc aggcccagca
    11761 ttcccgcagc cagcaggacg cgaaccagcg gccgtacacc ggaaggctgt cgcatggcaa
    11821 ggatcagcgt gctcacagat cacctccctt gctgccgatg actccggcaa aacatttgcg
    11881 ctccaggatc agcacctgca gcgccgggcc aaccacgagc agcatcagcg gaccgagcag
    11941 catgccgccc accaccacca cagccagtgg tcgctgcacc tgggagccga taccgtgcga
    12001 cagtgccgcc ggcaacaggc caatacatgc cgacagtgcg gtcatcagca atggccgcat
    12061 gcgctggtcg gccgcgttga acatcgcctc acctaccgac tgcccctcat tacgcaactg
    12121 gttgaaatag gtgatgacca ggatgccgtt catgaccgaa acgccaaaca gcgaaatgaa
    12181 accgatggcc gccgacacac tcaggtccag cccggaaaca tacagcgcga tgatgccacc
    12241 ggcaatcgag aacggaatcg ccaccagcgt cagcagactg tcgagcaatg agttgaacaa
    12301 cgcatagagc agcaccagga tcagggcgac ggctatcgga agaatgatca tcagccgttt
    12361 gcgtgcctgc ttcatttctt cgaattcacc cgcccagacc atgcgatacc cttgcggcaa
    12421 caccacgtga cgggctatac gcgcctgcgc ctcggtcacg gtgctgccca gatcgcggcc
    12481 gcgcacgctg aatttgaccg gaatgaaacg ttcgccacgt tcatgataga tataggacgc
    12541 gcccgtatcg agcgaaatgg tagccagttc gcccaacggt atgtagctga cgccacccgc
    12601 ggcattctgg taacccacgc ggatacggcg cacggcatcg atgttctggc ggaattgcga
    12661 agcatagcgt accgtcaggc caaactggcg atcgccctcg taaatcgtgc tggcctgcgt
    12721 tccccccaat gccgcctgca ccaccgcatt gacatcaccg ctattgaggc cgtaacgcgc
    12781 agcgcgggcg cggtcgacac gaatattgag attgggctga cccatcacgc ggaacacccc
    12841 cagatcctgc acgcctttga cctgggacat ttcgtgcagc acctggttgg cgatggattc
    12901 cagtacgttg agatccgggc cgatgatctt gactgcattc gcccccttga ttcccgacag
    12961 cccttcctcc acattgtcct gaatgtactg ggagaagttg aactcggtgc ccttgaactc
    13021 gttgtcaaaa tcgtgctgaa ggctggcgac cagcttttcc ttggtcatgc cagcgggcca
    13081 gtcgtcaaat ggcttgagtg gtacgaaaaa ttccacattg ttgaaaccgg aggcatcgct
    13141 gccgtcatcc ggccgaccat gctgcgacac gacggtgatc acttcgggat agctgtcgag
    13201 gatctgacgc atgcgggtca cttccgcatt gccggccttg agggaaatgg tcggcggcag
    13261 tgtggcacga atccacaggt ttccttcttc cagcgcaggc aggaattccg agccgctcaa
    13321 tgacaacagc acaccggcca acccgaggaa cgccatgccc agcaccacgg ttttcacttt
    13381 gtgccgcagc gcatgatgca gcaccggctg gtacaggtga cgcagcaggc gaaccagcaa
    13441 tgtctcggtt tcctcgacgt gtttcggcag caggaatgac gccagcaccg gcgtgatggt
    13501 gaacgtggcg atcagcgcgc cggccagcgc gtagccgtag gtgcgtgcca tcggtccgaa
    13561 aatctgcccc tcgacgcctt ccatggtgaa cagcggcaaa aatgcggcca cggtgatcat
    13621 gctggaaaac agcacggcgc gatcgacctg cagcgcgctg acgaaaatca gccgcaaccg
    13681 gtcagtccac tgcagatcag tctcgtcacg ccttccgcct gggccctgcc cggaaaactc
    13741 ttccagcagc atgttccgct cagcatcgat cttctgaaaa ttgcggaaca cgttctccac
    13801 caggatgacc gccgaatcga cgataatgcc aaaatcgact gcccccagcg acaacaggtt
    13861 ggcagactca ccgcttagca ccagcatgat gatgctgaag aacagcgcaa acggaatatt
    13921 gacgctgacg atgatcgcgc tgcgcaggtc accgagaaat acccactgga tgaagaacac
    13981 cagcagcacg ccgaacacca gattgtgcag cacggtatgg gtcgtcaccg ccaccagcgt
    14041 cgaacggtcg taatacggtt ccacacgcac gcccttgggc agggtgccat cgctgttgat
    14101 acgcgcaatc tcttccttga cccgcgtcac cacctcactg gtctgcatgg tgcggttcat
    14161 caccaccaca ccggtcacca catcgtcttc ggcatcgcgt cctgcctgcc ccagccgggg
    14221 cacatgaccg atactgcagc tggcgacatc tttcaccagc accggcacgc cgttgctctg
    14281 cgacaacacg acgtttccga tgtcctgcac gctgtggatc aaccccacgc cacgcacatt
    14341 gaccgactgc tgaccgaatg cgatggtgcg cccgcccaca ttgatgttgg cattgcccag
    14401 cgcagtcagc aactgcggca gcgtgacgtg atacgcttcc aggcgatgcg ggtcgacctg
    14461 cacctgatat tccttcgtgg taccgcccca ggcaaccacc tgcaccacac cgggcacgtt
    14521 gagcagacgc cgttgcagca cccagtcctg caatgtacgc aaattgctca gtccgaaatc
    14581 cggcggtccc ttgagctgat aacgataaat ttcaccgacc agactggatg cctgtatctg
    14641 tggctggacg ttgttcggca ggctgacatt ctgctgcagg ctcaacgcag cctgggtata
    14701 ggcaaagtag taatcgacgc catacttgaa agttacgcgc acgaatgaca ggccatagaa
    14761 cgaggtggag cggatgctgt ccactcccgg cgtcgtggcc agacccactt ccatcggaat
    14821 ggtgtaatag cgttccattt cctcggccga cagtcccggc gcctgcgcgg tgatttccag
    14881 gatcaccggc gccgggttgg gataggcttc gacattgagt ttgtagaacg ccgccagccc
    14941 gaccccgatg aacaccagca ggaacaggat gatgatcgga cgacgggtga gcgaaaagga
    15001 aatcagaccc ttgagcacgg ctgtactttc agcgaatcgg taaataacac gggcacaacc
    15061 tgctcaacgc aggcccagca cccaggcatt gctgatgaac agtgcgccat cgcccgcaat
    15121 cagttcgccc ggtttcagcc cggccagaat ctgatccatg ccgtgctgct gcatgcccag
    15181 ttgcacgtgc cgacgataaa aatggcgccg gtccgtcgtc acccagaccg tcatgctgcc
    15241 atcgccctca cgcaccacgc cattgaccgg cacggcagtc gccagcgtgc ttctgcccgc
    15301 atgcaggatg aaggtcgcca tcatctgcgc ggcaatgcga tgccgaggat cctgaactgt
    15361 gctgcgcacc gccacgcgat gcgtattcgg atccaccgaa ctggccacgt agtccacccg
    15421 gccgtgaaac acttcgtcgg gataagccat cagccgcaca tcgaccgcct gaccgacatg
    15481 caccagcggc agatcggact cggccacgct ggccaccagc cacttcctcg acagatcgga
    15541 caccgtaaac ggcgccgggg tcgcacccgg ctgcaccagc agaccgggcg cagcattgcg
    15601 cgccaccacc tgtccactga tcggactgag caccggcatg acggaatcga tgtgccggct
    15661 ggcgacgatc tggcgaattt gcgcatcgct cttgccgaag atgcgcagcg cgtccgtcgc
    15721 cgacctgtag gccgcctcgg ccgtctgctg gtcggacaca gcctgctcgt agtctttctg
    15781 cgccattcct tgcaccgcgt agagttccct cgcacgctga agcgcggcat cggtcagatg
    15841 cagcgtgccg gcgctcgaca acagcgcaga ctcggcctgc accaggtcgg gactgtcgat
    15901 gctgaacagc acctggccct tgtgcacatc gtcaccggca ctgacgctca ccgataccac
    15961 ccgtccggcg tacggcgacg agacctgcac cgtacggtcc tgatcgtaat ccgtgttgcc
    16021 cacggcctcg cgttcgtcgg cgaaatcggc ctggccgacc cgaaccacgg aaatgaattt
    16081 cgtctgtccg tccgtcagcg tgacttctcc gccggtgctg gccgccatcg ccccggcaga
    16141 tgcaaccagc agccagacag gcagcaacag accggccatc cggacgcatg gcctgcagcc
    16201 aaaaagagat gatttcgctc gcatgaaacg cctcgataaa tacgcatgaa gccggcgctg
    16261 tcctgcgcca gccgaaagac cgcgacattc taaccgaaga tcggcaaccg gcaagtattt
    16321 gcctgattac agtttggcaa gattgatcgg cagcgcaggc tgcaccatca tccggccggc
    16381 tgatgcggca gcacgatgcg cgccagcaaa ccacgcccgg tattgagcag ggcaaggcga
    16441 ccaccatgca gggaagcaat ggcggaaacg atggacagac ccagcccgtt tccaggcagg
    16501 ttgcggctct ggtcaatgcg gtagaaccgg gtggtcaccc gcggcaattc ggcctcgggt
    16561 atccccggcc cgtcatccag cacttcgagg aacacgtgct cgcccgcatg cacggcacgc
    16621 acctcgaccg tactgtccgg accgctgtat ttgatggcat tgtcgatcag gctggccagc
    16681 gcattggcaa tcaggttgcg atcgcccagc gccagcaccg ggccgccgtc cacagcacgc
    16741 aactgcacac ccgcggcttc cgcactggca tcgtacagat cggccatgtc cctggcgacc
    16801 cgacgaatgt caaccggttc gaaagcattg gcatgcactc ccgattctgc ctgagcaatc
    16861 tgcagcagct tttcaaacac caggatcagt tcatcgatct cgccaatggc agcaaccgct
    16921 gcttcacctg ggctgcctgc gccatctccg ctgcgcacag cttcttccag ccgggaccgg
    16981 atgcggctca gcggtgtgcg caggtcatgg gcgatggcat tggatacatg cagcactcca
    17041 tccatcagct gttcgatgcg atcgagcatg cggttgatgt ccattgccag ccggccgaat
    17101 tcgtccgaac tggcgaccgg gatacgccgg ctcagatcgc ccgcctcgat ctcatgagcg
    17161 gttctgcgca tgacggcgat cggatgctcg atcagatgac gaaagaacaa cgccccgcca
    17221 aaaatcagca gcagcgagat cagcgcgcct gcagccagcg accgcagcac cagtcgcccg
    17281 atgagttcct gctcgctcaa ttcccagccc acgatgagcg tatcgccgtc aagcaatgaa
    17341 atccgcaaca gacgcgccaa tgccggcctg ccatagcgca acacgtgcgc cgttaccggc
    17401 ccgcgatccg gcagtgaggg ccaggtatcg agattgccca cgatccgctg ccctcgcgac
    17461 gaaagcaact gaaaaatttc cgtatcactg ttgatgctgt cacccaactg tctgtcaatc
    17521 tcgcgcgcca gcgcaccagg atgatccata ttgaaccgga tctcccgatt ggcaatataa
    17581 atcagtcggc gatcgacatt ctgttgcagc acacccatcg tgccgaaata gaacaccaac
    17641 gacaccacag cgatcgacgt ggccaccagc aagccgtaac cgagcagcaa ctgtgtcacc
    17701 acagaccggc ggatatcagg caccacggtc tccggcaacg gctgacagcc ggtaaccgac
    17761 accgcgcacg gtatggatca gtgcgacgtc aaactccttg tccaccttgt tgcgcaggcg
    17821 gctgatctgc acgtcgatca cgttggtctg cggatcgaaa tggtattccc acaccgattc
    17881 gagcaacatg ctgcgcgtta ccacctgccc ctcgtgacgc atcaggtatt cgagcagacg
    17941 gaattcgcgt ggctgcaaca gaatgggaca tccatttcgt gtcacccgct ggctgagcag
    18001 atcaagctgc agatcggcca cctgcagacg cgtttctccg ttacctgtcg gacgccgacg
    18061 tgtcagcgcc tcgacgcgcg ccagcaactc atcgagcgaa aacggtttga ccaggtagtc
    18121 gtcggcaccg cagcgcagcc cgtgcacacg ttcatcggta ctgccaagcg cactcagcat
    18181 caacaccgga gtgcggtcgc cgcgggcacg catgcgttcg agcaatgcca tgccatcagt
    18241 cgagcccggc agcaggcgat caaggatgac gacatcccat gccgaggcat cgaccagttg
    18301 ctgtgcttcc acggcattgc cacacggcac ggcgacgaat ccggccgtat gcagcccgtt
    18361 gcacagataa cgcgaggtgt tgggttcgtc ttccacgacg agacagcgca tgaatcagtc
    18421 gctccgcagc gcaggatgac ccggcagatg acatgggttt gacgtcagcc aggcttcagg
    18481 gtaaaattcg cgtctctcca tagaccagag gtaagcgacg ttggactgtc acagttgtaa
    18541 caaatccttc tgttattgta cacgctagcc gcactcaccc tccagttttc ctgaactgcc
    18601 cgggagtctg ccgctggcaa acggtggaac ccgggctgcg ccccagtgtc cgacgcacag
    18661 tcgggcacag actgctcccg tattcctgaa attgcgccca ccgcaagcac gcggctgcgc
    18721 gcgtgttcgt ggcgttggca tggataacga aaagagcgca tcatgaaaat ccggcagttt
    18781 tttcccggcc tggcagccgg catcgtctcc ctgttcccgg tcaagggggg ctccgccgcg
    18841 cgaccagtac cgcccgtgtc cgcccgcttg ctcgaagtgg cgctgctcac ccccgatgcc
    18901 gcactggtca agctcgacag ctccagcgaa ggtctgctcg accgcgaagc cgaagagcgg
    18961 ctgtaacagt acggcgccaa caccgtcacc cacgaacacg tcaaatcggt gtccgagcgc
    19021 atattcgaac agttcaggaa cccgctcaac atcctgctgc tgacactggc cggcgtttcc
    19081 tatttcatgg gcgacgtcga atccagcatc atcatttcgg tgatggtgct gctcagcgtc
    19141 accctcacct tcgtacagga ataccgttcc agcaatgctg cagaaaaact gcgcgccatg
    19201 gtgagtacca ccgccaccgt gatccgcaag gacaagcgcg ccggcattcc gcaggaagtc
    19261 aatgatgcgt acaacatccg catcgtgccg catggcccgg aacgcaagga agtgccgatc
    19321 accgaacttg tgcccggtga catcatccag ctttccgccg gcgacatgat cccggccgac
    19381 gcgcgcatcc tcactgccaa ggatctgttc atcaaccaat cctcgctcac cggcgaatcc
    19441 atgccggtgg aaaagttcgt ccacgccagc aacccgtcgg tgcgcgaccc gctgcagctc
    19501 gacaacgtct gcttcatggg caccaatgtc atcagcggca gcgccacggc cgtcatcgtc
    19561 atgaccggcg ctgataccta ctttggcggc ctggcaagcg tggtgtccgg cgaacgcgtc
    19621 cagaccagct tcgaccacgg cgtcacccgc ttcacctggc tgatgctgcg cttcatcctg
    19681 gtcatggtgc cgctggtgtt catcatcaac ggcctgacca agcacagctg gatcgaagcc
    19741 ttcttcttcg ccatggccgt ggccgtcgga ctgacgccgg aaatgctgcc catgatcgtg
    19801 accgtcaacc tcggcaaggg tgcgctggcc atgtcgcgca aaaaggtcat cgtcaagcgg
    19861 ctcaactcga tccagaactt cggtgcaatg aatgtgctgt gcaccgacaa aaccggtacg
    19921 ctcacccagg atcacatcat tctggaaaag cacctcgaca ttttcggcga gcaaagtcag
    19981 gcagtgcttg aatacgctta cctgaacagc tactaccagt ccgggctgaa aaatctgctc
    20041 gacgtggccg tgctcaagca cgtggaaatg aacgaacacc tgcatgtcga gaaagatttc
    20101 cgcaaggtcg acgaaatccc cttcgatttc gctcgtcgcc gcatgtcggt cgtggtggag
    20161 ggccagggca agcacctgct gatctgcaag ggcgcggtgg aagaaatctt caatatttgc
    20221 gccgacagcg aaatcggcga cgagaaattc ccgctcgacg acagccattt cgcccggctg
    20281 cgctcggtca cacagacact caacgaagac ggtttccgcg tcatcgccat cgcctacaag
    20341 gagatcgacg gcaaacaggc cagctacagc attgcggacg aacgcgatct gacgctggtc
    20401 ggctacatcg ccttcctcga tccgcccaag gaaacggctg ccgccgccat cgccgcgctc
    20461 aaccggcacg gtgtggcagt gaaaatcctc accggcgaca acgacatcgt cacgcgcaag
    20521 gtgtgcaaga gcgtcggttt gcacgtcgat cgcatcgtgc tgggcagcga agtcgaaaag
    20581 ctcagcgata tcgaactggc agaactggcg gaacggatcg acgtgtttgc caagctgtcg
    20641 ccaacgcaaa aagccaaggt gatcaaggca cttcacctca aagggcacgt ggtcggcttc
    20701 ctcggcgatg gcatcaacga cggcccggcg ctcaaggctg ccgatgtcgg catttcggtc
    20761 gacactgcgg tggacatcgc caaggagtcc gccgacatca tcctgctgga aaaaaacctg
    20821 atggtgctcg aggaaggtgt gatcgagggc cgcaaagtgt ttggcaacat catcaagtac
    20881 atcaagatgg gagccagctc gaacttcggc aacatgttca gtgtgctggg agccagcgcc
    20941 ctgctgccat tccttcccat ggcgccgatc cagatcctga ccaacaacct gctgtacgat
    21001 ttctcccaga ccgcgatccc gaccgataac gtcgatgagg aatacatcgc ccagccgcgc
    21061 aaatgggata tcgccaacat cacccgcttc atggtcatga tcggcccgat cagttcgatc
    21121 ttcgactaca tgacctacgg catcatgtac ttcgtgttcc acgcccacac gccggaacag
    21181 gcgccgctgt tccagaccgg ctggttcgtc gaatcgctgt tttcgcagac gctgatcatt
    21241 cacatcattc gcaccggcaa ggctccgttc ttcgaaagtc gcgcaagcct gccgctcatg
    21301 ctcaccagtc tgatcatctg cggcatcggc gtatggctgc cgttttcgcc cttcgcccac
    21361 gcactcggct ttgtgccgct gccgacgcgc tactggtatt accttgccgg aatgatcctg
    21421 ggctacgccc tgctgaccca caccgtcaag atgtggttcg tgaagaaata cgggctgaac
    21481 tgacatgata cgtgcccgca cgatgcgggc acgttgtccc gctacgcaat ggcggcagac
    21541 cgcgcgcgtt tcattaccag gccgttgaaa aaggtagcga ggacgcccgg atgcaaggcg
    21601 cgagaaacgc aacgacgaga cctatcaaac agataggcgt tggagtgagt atcgcagcaa
    21661 cgccgcagac gggtgccgca gtactttttc aacggcctgt tacaggagac tgccatgcac
    21721 cacctgaccc cacgggaagc cttcgaattt ctcggaaata ccccggatgc ggtattcatc
    21781 gactgccgct cggaactgga attcctcttc gtcggccacc ccgccaattg cctgctgatc
    21841 ccctggtatg agggagcgaa ctgggaactg aacccgcgtt ttctgaatga agtaagtgct
    21901 gtggccgagc ctcatcgccc ggtagtgctc atctgccgtt ctggcaatcg atcccgggaa
    21961 gccggtgaat tcctggaaaa aaatggcttc accgacatct acaatgtcac atccggtttc
    22021 gaaggcgaac tggacggcgc ccatcaccgc tccacgcaga gtggctggcg ctttgacggt
    22081 ttaccgtggg agcaatgctg aacaccgtca ccggggccgg gatctgccgc ctgcaaactc
    22141 cgctgcggca cgacaggaca cgcgatgctg gttcgcctga ttcgggcagg atcaaaccgg
    22201 actagtaaat gcgtggttgc ggcacaaacc ccggcatgct ctccggctgc aggttgaccg
    22261 gcttgctgcc caggcggtca tcctgcagcc aatacaggct gtgcaccatc cacgaggtat
    22321 cgtcgcgctg cgggtagtcc tcgcgagtat gcgcgccacg actttcgcaa cggaatcggg
    22381 cgctgaacag cgtcgcccgc gccacctgca gcaggttgcc gaattcgagc gcctcgatac
    22441 gttcgacatt gaacgcccgg ctacgatcgg tcagtaccag ttgctgtgca cgctgcgaca
    22501 gcgcatcgag ttcgcggacg ccctcgtcga gcaatacacc gtcgcgaaac actccgcaat
    22561 ggcgctgcat gaggcgctgc agtgcgtcgc gcaggcaggc caccgtttcg cccgtgcccg
    22621 gccggtcgag ccgcgccagc agttcccagt ccgcctgcgc cggcggtagc ggagcgtcca
    22681 ccgcaattgt gcgtgccgcg tgctcgccgg cagcacggcc aaatatcacc agatcgagca
    22741 gcgagtttcc ccccagccgg ttggcaccgt gcactgacac acacgcacac tctccgatgg
    22801 catatagtcc gggcaccgga tcgcggtcga gcacctgacc gtgcagattg accggaatgc
    22861 ctcccatcat gtaatgcgcc gtaggcacca ctggcaccgg agcctgcacc ggatcgacat
    22921 tggcaaaacg catggccagt tcgcggatgc ccggcaaccg ctcgcggatc accgcctcgc
    22981 ccaggtgatc aagccgcagg tgaatgtatt cgccttgcga accgcatccc cgacccgcca
    23041 gaatctccat tgccagcgcg cgtgccacca catcccgact ggcgagatcg gctgcatgcg
    23101 gcgcataacg caacatgaac cgctcgccgg cagcattgag caggtaaccg ccctcaccgc
    23161 gcaccgcctc cgaaatcagg ctgcccgaac cgggcagacc ggtcgggtgg aactgccaga
    23221 actccatgtc ctgtagcggc aatcctgcac gcaacgccat accgggtccg tcgccggtat
    23281 tgatgtgggc gttggtcgaa tgacggaaaa cgcgcccagc gccaccagtg gccagcaccg
    23341 tggccgcggc gtgaaactcg tgccggccgg cgtcggcaat cgacagcgcc gcaataccgg
    23401 tcacctcacc ctgggtcgaa cgtaacaggt cgagcgcaaa atattcgtcg aagaaacgag
    23461 tccctgcccg caggttgcgc tgataggggt agacgcagaa aacggaattt aaagcactct
    23521 aagccaaacc atgtcattga ttt
//
LOCUS       DAIANL010000152        12471 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1625945, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000152 DAIANL010000000
VERSION     DAIANL010000152.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 12471)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12471)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12471
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625945"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2380 a         3324 c         4005 g         2762 t
ORIGIN      
        1 cgtttctgag ttgctgattc agttcaaaca cttgcagcga gcgtgcgctc aggttttcat
       61 acagttcgct cacggtgtcg atgagcaggt cgcgctggct gtggtcgtcg ctgtcctcgt
      121 cattggccgc aaccgggtta ccgttgcgca gggcgcggat ttcccgggcc agtcgcacgt
      181 cgacgctgcc aatgtgatgt accagccatt tgacgagaaa catgagcagt gaatcgatgg
      241 catcggcggg ggtggtggca atcagtctgc ctgtctgttc gatgcggtcg atgaattcct
      301 ggtgggccct gagatgggca tgcttgcgaa ccgggtcgac cggccacgca tgcatcagct
      361 cggcttccgt ctggaaatgg taatgcgtgt agccgcgcag gtcgttcagt agcggcatca
      421 ggtcgggggg ggtggcgcca ttgcggtgca gttgcaccag cgcattgacg atgtcgacca
      481 gatggcgatg ctgatcatcg acattggtca gaccggtagt catctgttcg ttccattcaa
      541 tgatggtcat tgtcctggct caaacgtggg ttcgggcggc acgaagtggg tggccgaacg
      601 gggagggtac gtcaaatccc gtctgtcgtg caaagaaaat ttttcgatcc atggccgtgg
      661 cggcacggag accgtggcat tgccctacgc gctggtgccg tatagaatca gcttttccga
      721 aggcgggaca gcgggacagg tgtgtcatga cggatgacca tgctgccggt gcctgctttt
      781 ggccattcct gtcccgtgcg cccgtgatga acgactggtt cgcccttctg cgccgattgc
      841 gcagcgccgt acgtatccct ttgcccgaaa gggagcggct gcgctggctc cagggtgggc
      901 gattggcctg gctgctggtg gtgctgtttg ccatgttctt cggtctgtcg ttcacctggg
      961 tgtcgctgca cgccgaacgc gaagaaatgg cgcgcaatct ttcgttgttg gccgaactga
     1021 gcggcaagtc ggtcgatgct ttcttccagc agatcgatgg ccggctcgcg gtgctggccg
     1081 attcccataa caatacgggc gaaatgccgg cccggttgca actgcgtctt gcccgcttca
     1141 tgcgcttcaa tccacaggtg gtgcggatgg tggcgatcga tgcacagggc aatctgctgg
     1201 cggctgccgt ccagggagcg agcgacgcat ccggcaacga ggcgctcgtg cagtttgccc
     1261 gggcatcgcg acatgggtta ttgtccagca aacgtatgct gatcggtcat gcacggtacg
     1321 atgtggccgc gcgtctgtgg gtgattccgg cgctgtacgg cgtgcgtgac ccggacggtc
     1381 aggtgcgcag actgtttgtc ttttcgatgc gcgccgagca gttgcaggat ctgtggcaaa
     1441 gcatgtacct gccgacacag gcacagatgg gcctgctgct cgacggtggt tatttgctga
     1501 gcatctatcc tccacccacg gcggccaaca gcgaacggat ttacgagcag ccgcgcagtg
     1561 gcgtgctggc acaggcactg cagcggccga cgtttgcgcg ccacggcacc atcgaaggct
     1621 acaatagcgt ggcccgcaag gcatattatt ttgcctggta ccacctcgac cgttacccgc
     1681 tgactttttt cgtggcgatt ccggtgacca acctgtacca ccgctggtgg agtcacaatc
     1741 agttttttta ctggcttggc atactgtttt tgctcgccgg cgctgctgtg tatcagttga
     1801 tggcgtcacg tacccgtggc tgggaacgcg aacgtgcgcg gcagagcgcg cggctggaga
     1861 cgcaacggcg ctggaatcaa cgcatcattg atgcatcgcc gatcggcatt gccatctatg
     1921 aacaatcggg caactgtatc gtcgtcaatc cggccatgga gcacctgttc ggcgatgact
     1981 gggggggcgg cccggccaga cgtcagtcgc atcgcctgca ggattgggag gaatttggtg
     2041 cgggcaccct gattgcgcag gcacagacgc aggcggagtc cagcgcggtg tccggcatgc
     2101 tggcattcag gcgtacagat ggaacgctcg tcaatttcaa catcatggtc agcatgcttg
     2161 aggatgaggg tgaacgccac ctgctgctga tggcaaccga cctgtcggaa accctgcgtg
     2221 tgcagcaggc attgcaggaa agcgaactca aactgcatcg ggttattgcc gttgcgcccc
     2281 tgccgatcat gattcatgcc gaagacggcg aggtgctgga aatcaaccat gcatggcagc
     2341 aggtgagcgg gtattcgttc gaagatattc caacggtggg ccggtggctc gacctggcgt
     2401 atggcgaggg tgcgtccatc gtgcgcgagc gtgtgaacat gctctacgct tccgagcagc
     2461 gtgtcgatca gggtgaactg ctcattcgct gcaagaatgg cgaactgcgt atctggcaca
     2521 tcgttgcaat ccaggtcgga catctggctg acgggcgtcg ttatgccatc agcacggccc
     2581 aggatgtgac cgaacgccgt gcggcgcaag atcgggtcga atttcttgcc tatcacgatg
     2641 cgctgaccgg attgcccaat cgcatcctgg tgcgcgagcg cttcgatctg gcgcggacca
     2701 ttgccgaacg ccaacgcaac aaagtggcgc tgatctatct tgatctcgac aatttcaagt
     2761 tcgtcaacga tacgttggga cacgtggtag gtgacaagct gctgcaggct gttgcgatgc
     2821 ggctgcgcga ctgcctgcgc gaatcggata ttctcggccg gcaaggcggt gacgaattcc
     2881 tcgtcgcgct gtgcaatgtg caggatccgg acgaggtggt caatgtgctg gaaaagatcc
     2941 agcagcagct cagcaacacc tttgtgatcg atggaaacga gttgtcgacc tcgctgtcga
     3001 tcggtgtcgc ggtctatccc gacgatggcg acaactacga tgtgttgctc aagaatgccg
     3061 ataccgctat gtaccagtcc aaggcggccg gccgcaacac ctggcatttc tacaccgagc
     3121 agatgaaccg ccattccgtg gaacacctgc atatccgcaa ttcattacgc aaggcgctcg
     3181 aacgcaatga attcatgttg ttctaccagc cgcagatcga tatggcgacc ggggcgatca
     3241 tcggtgccga ggcgctgatc cgctggcagc atcctgatct ggggctgatt ccgccgaacc
     3301 ggttcattcc ggtggcggaa gaaagcggca cgatgcttga aatcggcaac tgggtgatgc
     3361 tcgaagcctg ccgccaggcg cgtgcctggc agcaggacgg gttgggtgat ctggtggtcg
     3421 cggttaatct gtcggcggta cagttcaaac agggcgatct ggaaggcaat gtgctgcgag
     3481 cactcgaagc atcaggattg gcgccgtgct ggctggaact ggagctgact gaatcgattc
     3541 tgatccagaa cagcgaaatc aacctggaca gggtacgggc actgaaaaat ctgggcgtca
     3601 agctgtccat cgacgatttc ggtaccggtt attccagcct ggcctacctc aagcgttttg
     3661 ccgtcgacaa gctcaagatc gaccagagct tcgtgcgcga catgatggac gatcccaacg
     3721 atgccgttat cgtgcgcacg attatccaga tggcggtcaa cctcaatctg cagactgtgg
     3781 cagaaggggt ggaaaatgtc gagcagatgc gcatgctgcg cgatttccat tgcgaccagg
     3841 cgcaaggata tttctttgcc cggccgatgc cggctgccga atttcccggt ttcgttcgtc
     3901 agcatatgac ctattgaggt ggaatgccat caggataaaa ccatcctgtt ttgttgttga
     3961 atcccattct catttgtgta ataattctca ttaataaatt aacagagtcg acacaatggg
     4021 ttggtataat tctgaattcg gggagggtgg ggtgacaaat ttacttgata acctttgtct
     4081 gcaggcgggc aaaggggtgt gtctgctggc tttgggctgt ctgccatcgc aagcagtcct
     4141 ggcagatgcg cttacagcta cgccagcgtc accagtttca ctttccgccc cggcaccagt
     4201 tgccgtgtat ggccagtaca ccaacgtcta tcagtggcac ccggcattca cttcaccgta
     4261 tcagggaaca aacagcctgt cgcccggcaa caacggcgaa cagaccaacg atgcaacgct
     4321 gtatgccggc tatcagccgt cttccggcaa tgaattctgg atcaacgccg aatacgatca
     4381 gggctttggc ctgaacggca cactgggaat ggcgggtttt ccgagcggcg aggcatacaa
     4441 ggtcggggcg aacaatccgt attaccgtac cccacgactg ttctatcgca aaacgatcgg
     4501 tctgggtggc gcatcgcgca cggtcgaggc tgatatcaac cagtttgcgg gaagcaccac
     4561 ggccgacaac ctgatactga cggtgggcaa atttgccgtt accgacgtgt tcgataccaa
     4621 cgtgtacgca cacgatccgc gcaatgactt cctcaactgg tcggcggtcg atgccggtgc
     4681 ctttgactat gccgccgatg cctggggata tacctatggt gcggccgcgg aatggacgca
     4741 gtcgtggtgg acgttgcgcg gaggtgtgtt tgacctgtcg caggtgccga acagtgaaaa
     4801 actgacccag cggttcagcc agttcgaact ggtggtggaa gccgaggggc gttatcaact
     4861 ggcgaacgcg ccaggaaaac tcaagctgct cgtgtttgac aaccgcggcc gcatgggcag
     4921 ttacgccgat gcactggtgc ttgccgcgca aaccggcagc acgccggata ctgcgctggt
     4981 gcgtcgttac gcctcgcgtc ccggggcagc gttgaatctg gagcaatcgc tgggggaaga
     5041 tctggggatg tttgcacggt tgagcgcaaa caacggtggc gaagaaacct atgaattcac
     5101 cgacatcaac cagtccgccg aaattggact gtcgttgaag ggcaaccgct ggggccggcc
     5161 ggacgacagt gtcggcgtgg ccggcgtggt caatggcctg tcgggcgaag cgcgtgccta
     5221 tttcgctgcc ggaggactcg gcgtgctggt gggtgacggg caattgcccg attacgccac
     5281 cgaacgcatt ctcgaatcgt attatgcctg gcaggtgacc cgtcatgtca cgctgagcgc
     5341 cgactaccag ttcgtgcaga atccggcata caacgcgcag cgtggcccgg tcaatatttt
     5401 tgcctggcgt ttgcatgtgc tgtactgatt cttgtctgca attgcgtggc ccgacgttcc
     5461 cgccgcgccg ccagcgctga tataatcatt caaccgcagg ttccggagtc gttcgtgcac
     5521 gatcacgcgc atcagcatca ttcacaccac aatcattcgc atcagccagc tgggggggcc
     5581 agtggtcgca tgtttgtggc ggccatggcg ttcaacctgg cgttcgtcgc tgtcgagatc
     5641 gggttcggcc tgtatgccga ttcgctgtcg ctgctggcag atgcaggtca caatttcagt
     5701 gacgtgatcg gtctgttcgc cgcctgggtc gccatgctca tggccaggcg ccagccgacg
     5761 ccgcgtttca cttatggctt tcgcagttcg acgattcttg ccgcactggc caatgccatg
     5821 atcctgctga ttgccgttgg cggcgtgagc tgggaggcaa ttcagcgttt tgccacgcca
     5881 caaccggtcg atggcgcaat catgacgggt gtggcgctgg tcggtgtgtt cgtcaacata
     5941 tccacggcgt tgctgctgat gcgtggtcgc gaaaccgatc tgaaccagcg cggggctttt
     6001 ctgcacatgg tcagcgacgc tgccgtgtcg ctgggggtgg ctctgggcgg attgctcgtg
     6061 cgcgagacgg gctggctatg gcttgacccg gccatcagtc tgctgattgc gttgcttatt
     6121 tttgccggta cctggaacct gttgcgcgaa tcgctgcggc ttgcgctgca ggccgtgccg
     6181 gatcgcatcg atccgctggc agtgcgcgcg tttcttgccg ggttgccgcc ggtgaccgcg
     6241 gtgcatgacc tgcatgtatg gggcatgagt acgacggaaa atgcactgac cgtgcatctg
     6301 atgacgccag ccggtcatcc tggcgacaac tttctgcacc aggtggcgca ggatctttca
     6361 cagcgttttc atatccagca cgccacgatc cagatcgagg ttggtgacga tgcctgtgcc
     6421 tgtccgctgg cttcggacag cgtggtgtaa cgcccgggca acagcctgtg tccggacagc
     6481 gccggagaac acgccgtccc acattcctgc gtgacggcgt ggcggagctt tactgaccaa
     6541 tctggcggct gacctgattt tcctgctggt tgatggtgcg ttgctgctgc tgtgtgatgt
     6601 ggccattctg ctggctggcc attgcgcgtt cttcctggcg tatctgatgg tcctgctgat
     6661 gcagtgcctg cgcctgttgt ctgctcattt cgccattgcg gacttcattg tgaatgcgac
     6721 ggttctgctt ttccaggcgg tggttgacct gtttgcgacg cgggtgggtt ttctgccagt
     6781 gagtgtctgc cagtgcattg accgagcctg cggcggcaac cgaggtcagg acgatggcga
     6841 tgagggtgtg gcgggcgatc ttcattatgc gtctccatga gggtgggtaa acatatcatc
     6901 gtgatacatg gcaaataacg gaattctgga tggtctgttg accagaatcc tgttaccggg
     6961 tgtatcaaaa tgtttcggcg cgttgtggca caattgtgca gatcaaccaa cacagggagg
     7021 gcaggggaaa atgagtggcg attccggaat tcatgtgcat gcagcccatc acggtgacga
     7081 ccggctcagt cagtgggtgg cattgtttac cgcactgctg gcggcgctgg gtgccgtgct
     7141 cggttaccag ggcaatcgcc tgatgaatga ggtgctgctg gagaagaacc aggctgtgct
     7201 gtacaaggct catgctaccg accagtggaa ttattatcag gcggtgagca ccaagatgca
     7261 cctgatggaa atggccagga tactgtcacc ggcgggaaaa cagaccgcat tcgatgcttc
     7321 gatcgccaag tacaagtcac agaaaagcgg aatcaaagcg actgcggaac atttcgacgc
     7381 tgcatcggag caggcgaatg cacatgctga acagatgaat gcgccgcacg agcggttggc
     7441 gatggcaatg atcctgtttc agatagccgt ggcgctggct tcggtcaccg ctctgaccaa
     7501 gcggggttgg ctgctcggcg tagccggcat cagtgccgtg tgcgggattg ggttgtgggt
     7561 gtatggcatg gccatggtgt gaccgcagcc gtaacagcta cgcttgccag tctctcggta
     7621 aacattggcg ccggattgtc acctgttccg catggattcc ggtgtttcgc cattttccgt
     7681 acactatcct gcttgcgctc gcgagcatgt caaacggtct ggcgagccgt ggggccggcg
     7741 ctcgggatgt actattcggt ttgaaagccg ccgcgccgca gcgtaagcac cagtgtgtca
     7801 cggtgcgccg attcgccctg gggctggatg ggcgtcgatt cgtggatcat gcgtacgtca
     7861 tcgagcagca gcagcgtcca gtcttcaagc agcgtaaagc gttttccgac gctcgatccg
     7921 gcttcgaaaa tccggctttc accgccgatg acgttgtgcc ggtccaccag caggatggcg
     7981 acgaaatcca cgccatcgcg gtgcgcgcct tctggcgtcg gccggccgat gccatcggta
     8041 gtgtcgatgc ggaactgatg cgcttcgaca taccacggtt gctggccgcg caatgcatcg
     8101 caaacggcgc cgacgccgcg caacaacctg ttccaggccg gttgtgcgat ggtatcggct
     8161 tcaatgggtt cgaacatgcg gcgaattccg ccatgcaggg cattgtattc gagcggttgc
     8221 cagtgaaagc ggtgcggtgt ctgctcgatg gcgctgccgt tgaggatgaa gcacgaatgg
     8281 cggcgacgac ggtagtggcc gccatcgcgc agatagtggt cagccggcaa gcgatcccag
     8341 gaatccgcca gggctgccag ttcggcaggt gcattgcctg tcagttgcag ggtatcgtgt
     8401 ggtgataaaa ccgcgtatcc ttgctggcaa atgtcctgaa cgaaatgatc agcctggctg
     8461 tagtcgggag gaatgacgag catgacacgg gagactgggc aacgcggggt aggcgtcgag
     8521 tgtagcaggg tttcccgcgc catgccagcg atcgttgatg gagccgcccc atgcgcagcg
     8581 tgagcgttcg atccggggcg ggtggtattg ctgatggctg ttacctggga gaaaacacat
     8641 gcgtgcgctg ttgacgatga tactgatgct gcaaaccacg atggtgctgg cggatggctt
     8701 ccgcgtcacc agtcccgatc ttccgtctgg caacaagatg attccacaga cgtttgttta
     8761 taacggtttc ggctgtaccg gccagaatga atcgccggcg ctgaactggt cgaatccgcc
     8821 ggcgggcacc cgaagtttcg cggtgatggt gcacgatccg gatgccccga ccggcggcgc
     8881 cgggttctgg cactggattg tggtcaatct gccggccacg atgaccggtc ttgcgcgtgg
     8941 tgatggcgcc gtcaacagta cacacctgcc gcacggtgcc attcaggtgc gctccgatta
     9001 cggtatcgaa ggctggggcg gcccgtgccc gccagcgggc gaccggccgc accgctacaa
     9061 tttcaccgtg tatgcactga aagtcgatca tctcgacatc ccgccaggcg ccagcgcatc
     9121 tgaggtgggg ttcatgatca cccaggcagc gatcgccagg gcaacgctaa ccgcgcatta
     9181 cgcacgctga cacgaaacac taccgccgcc cacatgggcg gcggtctggt ttgtgcaagt
     9241 gctgcgtcag aaacgtgcat ccagcgtcac atagtaattg cgtggctggt tcatctgcat
     9301 ggtcgtgctg ttgtaatatg ccgtgacata gctggtgttg gtcaggttgt tgaccagcag
     9361 cgagagcgtg gcatccttga agccgaacga atcacccatt ggcgcatgct tgaggcggta
     9421 gctcaggctg gcgttgaccg tggtgtttgc cggcagggcg agcagcgtgt tgttcggata
     9481 gccaccgacg ctgtagtagg cattgtccac gcggatggcg ccgatgcggc cggtccatgc
     9541 ctgatcctgg taaatcgcgc ctgcactagc cgtatagtcg ggcgcatagg ggaaaggctg
     9601 atttagcgta tcgaaatgcg catgcagcca tcccaggttg gcatacatcg accagtgatc
     9661 gtcgatcagc cagttgttct gccacgagat gccttcattg acggccgagc cggcattggc
     9721 cagttcggtc agcgtgccgc tgccaccgtt gctgatggtc gacgacagga tgtaattgct
     9781 gaaattgaga cggaaagcgt cgagtgtgct gctccagttg ctgattttcc agcgggtgcc
     9841 agcttcccag gtgtcggctt cttcaggcgc cagattcgga ttgaatgtcg aggtgtagaa
     9901 ctggttgtag gctggcggat tggcgttgcg cgtgtaattg gcatataccg accagtcgcg
     9961 cgacagcttg tagttgatgc cggccgaagg cagcagaatg ctgaaattgg tgtcgaagct
    10021 gtagggttga ccggccgcct gggcgacgac gttatcgaag ctgcgtgtca tgtacagata
    10081 ctttgcgccc accaccacat ccagcgcagg gctgaagtga tgggtatagt tgacgtaggg
    10141 ttcggttgtg tcggtagtga cgtactcggt gtaattggag ccgacatagc cgatggtggc
    10201 gtcattgtag taacggttgt catgggtact gtcgttgtgg ttgtaccaga caccggcttc
    10261 aagcgtgttg gcacggtcgg acagcagcaa tttcagcgtg tcgccccagc gttgggtgac
    10321 gttgtaggca tgatcgagca atacgttgtt ggcagacggc gaaacgctgc catagccgcc
    10381 gacgggttgc agcaggtgct gggtgtaggt cgtggcactt gcgccgaaac cgttgccgtc
    10441 atagcggtag atctggttgg tgatgtgtgc acctggcgca agcgggatgt cgagcttggc
    10501 atacatggtc gagtcgatgt actggttgta gttataaccc gtgtattgcg tattcggagc
    10561 gccactggtc agatatgggt tgctgccgag caggttgcag ctattgccat acagcgccag
    10621 gcctgcggcc gtgcagccat cgtagtagct gaaggtttca tcgttgcgac tgtagaacag
    10681 tgtcagcgta gcaggcccga tctgggtcac cgacttggcc agcgtctggt actgggtggc
    10741 aggtgtattc tggaaatatc catccatgtg gacgttgttc tgataaagcc acagcgcagt
    10801 cggcgcgccc gtgttgctgc ccatcaggcc gctgttgaac agcatgccga cattgtgctt
    10861 gccgaacgaa ccggcaccga gcagcggttc aacaaacatg tcgggaccgg gatcgcgggt
    10921 gtaggcttgc accgaaccgg caaatgcggc ctgcccgggg atggcggccg aggctgcgct
    10981 cgaatagtac tggatgccgg aaagcagggc gggcggcacg aactcgttgg tataaaagcc
    11041 atagctgtca ttgtcgttga gcggcatgtc gtcgacggtg aagttgatca gcgttttcgg
    11101 cagaccgttg atgtagacgt tgtcgccagt catgctgtca ctgccggtct gcaccaccac
    11161 gttgggcagc gtggccagcg cctgcgtgta cgaggcggtg ggtggcagcg catccagtgc
    11221 accggcagtg atgttgactt gcgcctggct tgcgtccgga ttgaacaact ggatattgcg
    11281 tgtctgttcg gccggtgtct gctcaacgtt gaccggagcg ggggtggcga acatttgtgg
    11341 cgtggccggc gcgctggcgc tgccggttac ggtgccgagg ttgatgacat catccgcgac
    11401 agccggatgc gcaacggcca ccatcaggcc ggtgagtgcg agaaacaacc gggtacggtg
    11461 atatggcgcg tccggattcg cgctaacgga acgtcgggtc tgcatgcttc agctctctgt
    11521 cagttgtaat gctgagagcg taagcaaacc gtatgataag aagatgaatg gaatattatc
    11581 gatttatgat gcagccgccc cggcacaacc ggggcgattg gcgggtgcag atgcagccgg
    11641 gtgcggatgc cgcgctgatc tcaggtgcgg atcggtgttt cgcccgtggt ctggcgctgg
    11701 ttggctggca ggcgcagcca cacggtttcg gggcgattcg gcgcggcagc aggatgttcc
    11761 gcacgatgtt catggcttgt ttcgggcagt agcagggccg ggtgccagtc cgcgggcgtg
    11821 gggcgggtct ggaacgatgg cgcaatgctt tgtgcgcggg cgcccgacgc cagcagcaga
    11881 gcgacgaaca gtaccgtggt cagaacgaaa gtgaacaggt ttcgcagcat ggtgatgtcg
    11941 gcctttggat tgaagggcgc cattggtggg ccctgcagca gaatttgctg accattgtgc
    12001 ctgattctgg tgtacagagt atttcagttc tgcaaaagat tgttacatcc tgttactgaa
    12061 tcatggactg caagacagga caagacaagt gcaaccacag cacgtttgtg ccacctgcaa
    12121 caatgggttg tggtgcagcg tgccgataga gacgtgttgc gctgaatctg cgcattggtg
    12181 cgcgcatcgg gctttacaag cgtgatgagc caatttataa tggcggactt ttgggggtat
    12241 agctcagctg ggagagcgct tgcatggcat gcaagaggtc agcggttcga tcccgcttac
    12301 ctccaccaga aagaaatggc agtaccacgt ccccatcgtc tagaggccta ggacaatacc
    12361 ctttcacggt atgaacccgg gttcgaatcc cggtggggtc gccaggttgc acaggggttg
    12421 cttgacggca accccttttg tttcggtttc gaacacggag tggtagttca g
//
LOCUS       DAIANL010000153        35783 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_195797, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000153 DAIANL010000000
VERSION     DAIANL010000153.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 35783)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35783)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..35783
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195797"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         7490 a        11817 c         9874 g         6602 t
ORIGIN      
        1 cgatgtggcg cgcgcagcga acaagcccca actgggtttc atgctgcgcc aggactggaa
       61 tgatgcgcaa ctcggtttca acgccaactc gtataccatc ggcggttcgc tgagctggac
      121 tgctttcgac ggcgggctga ccaacggcaa gatcgaccag gcacaagcca gccgtaacga
      181 gacatttgcc cggctgcaac aagccgaagc cggcattgcc gtgcaggtgg ccgatgcgca
      241 acgcaaggcc accgaagccg agcaacggct gacgcagcgc gaactggccg tcacacagat
      301 ggaagaagcg gtacgtctgg tcaacaagcg ctatgccaac ggcgtggcca ccatcaccga
      361 acagctgggc acccaggctc aactcgacaa ggcgcgtgcg gatgttgtca gcgcacgcta
      421 tgacctggcc atccagcgcg cccgcctgaa actcgccacc ggacagcttg atccggacac
      481 gctttaagaa tccgaattgc aggagcctgc cgtgaaccac tttgcctctc gccccctcgt
      541 cctgaccctg gctgcgacac tgctgcttgc cgggtgcggc cattccaccg aaaatgccgc
      601 cgctccgggg tccggtgcgt ccgtgcccgc cgaggtcatc accatcaatg agacgaatct
      661 ggcacagacc gccaccttcc ccgctggcgt cgtcgccagc gaacagatcc agatcgcctc
      721 gcgcatcatg ggctatgttc gtcagatcaa cgttgaaacc ggccagcatg tcaaggccgg
      781 ccagttgctg atcaccgtcg acccgaccga cattcagggc caggtcagtc tggcgcaggc
      841 cagcctggca caggccgaag cagctctggc cgatgccaag gccgattatg accgctttgg
      901 tgacctgtac cgtgaagaag ccattcccaa ggcccaatgg gacaagatcc gcctgcaata
      961 tgccgtggca cagcaacagg ccaacgctgc ccgcgcaggc catgccacgg ccgaagcaca
     1021 gatgcgctat gctgcgatcc acgcaccatt cgcgggcgtg atcacccagc gcatgaccag
     1081 cgtcggcgca ctggccgcac ccggacagcc gctgctcatg ctggtcaacc cggccagcct
     1141 cgacatcgaa acccaggtgc ccgaagcggt atacgcccgc ctcaaggtgg gtgaaaccgt
     1201 cggtctgcac gtgaacagcg ctcatctgaa cggcaccatc cgcgctctgg tcccaagtgt
     1261 cgacccgatc agccataccc acctggtcaa gattgcgctg cccgcagccg gccagttgca
     1321 aagcggcatg ttcgccacgg tcgacttccg catcggcgag catcccgggc tgcgcctgcc
     1381 ggccagcgcc gtcatcgacc gcgccggtat cccgggcgta ttcgttgtcg atgcgcaagg
     1441 catcgcacaa ttccgcatgg ttcgcccggg tgacactgtt gacggacaga cgaccatcct
     1501 cgccggcctg gagaacggag aacatgtggt actgacccca ccgaccggac tgcagaccgg
     1561 cgatcatgtc gtacgtccgg gagccggcaa tgtctgaaca cgtcacgcca aaactcaacg
     1621 tcgccggcag gattgcgcgc acgttcctcg aatccaagat cacggccatg atcatgctgg
     1681 cggtcacgct ggcgggggtc atggcgctgc tcgtcacacc gcgcgaatac aacccgcaga
     1741 tcgtcgtgcc cgcggccaac atcatcgttg ccaaaccggg cgcatcgacc gaagaaatcg
     1801 agaacctggt ggtcaagccg ctcgaagcgc tcatgaacgc ccagtccggc gtcaagcaca
     1861 cctttggtta cgccgtatcc gattatggcg tggtcacggt acagttcgat gtcggtcagg
     1921 accaggaacg cagcctggtc aagctctaca accagctcat gcagaatttc gaccgtatgc
     1981 cgagcggcac catgcaaccg atcgtgcggc caatcaacgt cgacgatgtg cccatcatga
     2041 cactcacgct ggcttccgca accctgccgg atgaccagat ccgccaggtt gccggccacg
     2101 tgctcgaaca gttgcgcaac atccccggcg tctcgttcac cgaactttcc gggggagcac
     2161 agcgtgcagt caacatctgg atcgatcctg cgcggctggc cgccagcggc atcagcctcg
     2221 accaggtcac ccgccagctg cagggtgcca acgtgtcact gccggttggc aacctggtcg
     2281 acaacaatcg cgaacacccg ctgcgggtga gcgggtttct cggcagtccg cgtgaagtcg
     2341 gcgacatcat catcggcgcg ccatcgggca agccagtcta cctgcgcgac gtcgccacca
     2401 ttcaggaagg cgctgccgaa accgatcagc tgagccgcct ggcctttgga ccggcaacgt
     2461 cccatccggc atggcatggc gaagtccccg ctgtcaccat cgccctggcg aaaaaagccg
     2521 gcaccaacgc tgtggtcgtc gcccacgcca tcctgaccaa gctcggtgaa ctcaagcgtg
     2581 acatgatccc ccagggcatc agcgtcacgg tgacgcgcga cgacggcgcc aaggccaatg
     2641 acgctgtcaa caccctgatg gaacacctcg gcatcgccgt cggctcggtg atcgtcattc
     2701 tggtgttctt tctgggctgg cgtgaagcgg ccatcgtcac catgaccgta ccgctcatcc
     2761 tgttcatcgt actcacgatc gggctgattg ccggacagac catcaaccgc atcacgctgt
     2821 tcgcgctgat cctgtcgctc ggtctgctcg tcgacgccgc cattgtggtg gtggaaaaca
     2881 tccaccggca catgcaccac ggcgacggca aaaacttccg cgaagcgctg gtcatcgcca
     2941 ccaacgagat cggcaatccg accaacgtgg ccaccattgc cgtgatcctt gcctttatcc
     3001 acatggcgtt cgtcaccggc atgatgggac cgttcatgca gccgattccg ttcaacgtgc
     3061 ctgtggccat gatcgcttcg ctgttcattg cctacatggt cgtgccctgg gccgcaaacc
     3121 gctggcttcc cggcaagacc ggcaagcaga tgagcgagac cccgacgctc gaggaactgt
     3181 cgagcaaccc gcaggatccg ttgcatcgcg cttatgtcac ggccatcacg ccgttcattc
     3241 atgacggccg gcgccgtacg ctgctgttcc ttgccgtgat cggcctgctc ctggcagcga
     3301 tgctgcagcc ggcctggcag ttcatccgcc cgtcaggcat gaacggcccg ctgtcgccac
     3361 tgggtgtcga actgaaaatg ttgcccaacg acaacaccaa caccctgctg ctgcaagtcg
     3421 acaccccggc cggcagcacg ctggtgcaga ccgaccgggt ggcgcgcgca gtcggcgccg
     3481 tgctgcgcgg cacggcatgg gtcaccgact accagacctt ccttggcatg accgcgccga
     3541 tcgacttcgc cgcactggtg cgcggcgaca tgctcaagcg cggcgacaat ctggcgcaac
     3601 tgcgcgtcaa cctgaccagc aagcacgagc gaccggtgtc atcgcatgac atcgcccgca
     3661 gcctcaacat tgcgctcgac gccgtgcgca gccagttccc cggcacgcgc atcaagctgt
     3721 acgaaacccc acccggaccg ccggtacagg cgcagatcct cgccgagctg tacggcccgg
     3781 actacaacac cctgcgctcg gccgccgcta cggttgaccg cgacttcaat cgcatctatg
     3841 gcatcatcaa caccgacgac agcgtcactg gcagcgtaca gtcctggcat atccgcatcg
     3901 accgacagaa ggcggcactg tcgggtgtcg ccgtgggcca ggtagccaag ctgctgcacg
     3961 attacgtgtc gggtttcagc atcggttcac tgcacgatcg cggcagcctg gaaccgatcc
     4021 agctgaccgt gcgtctgccg cgcagcgagc ggcaaacccc cgagcagatt ctggcgctgc
     4081 acatcacgaa catgaccggc cagcagatcc cgctctcggc gattgccagc atcgaaccgg
     4141 ccacgcagga caaaccaatc tatgaccgcg accagcatcc gatggtcatg gtcggcggcg
     4201 aactgctgca atcgagcccg gtgtacgccg tgctggctct ggacaagatg ctcgacggca
     4261 aacgtctgga caatggcgcc accttcacca ccggcaatct cgggtttgtc gaaacccagc
     4321 cggatgacat cgtccgctac catctgctgt ggggtggcga aatgcgtctg acgctggacg
     4381 tgttccgtga cctcggctcg gcattcatcg tggcgctggt gttcatctac ctgatgctgg
     4441 tgggctatta caagtcgttc gtcatgccac tcatcgtcat gggcgccatt ccgcttacgc
     4501 tgatcggcgt gttccccggc cattggctga cccatcaggc attcaccgcc acctcgatga
     4561 tcggggtgat cgcgctggcc ggtgttgtgg tgcgcaactc attgctgctg atcgacttca
     4621 tcctcgacta ccgctcgcag ggctattcgc tcgagcaggc cgtcatcgag gctggcgcag
     4681 tgcgtttccg ccccatcatg ctcaccgcac tggcgatcat tctcggctcg gccatcatga
     4741 tcaccgaccc ggtgttcgga gggttggccg tttcactgat ttttggtacc tttgcatcca
     4801 cggtactgac cctgatcgtc atcccgctgc tgtacctcat ctggcaacgg cggattggcg
     4861 ttacccccgc accctcccca accccctgac acacaggaga ttctcatgac cgtcgaacgt
     4921 atcgtacgca tcgtcgcagg cagctttatc atgctatcat tggcactcgc gcattttacc
     4981 ggccaggccg atctcagcca cataagctgg ctgtggttca ccgcctttgt cggtttcaat
     5041 ctgttccaga gtggaatcac ccgcttctgc ccgatggaca tcatgctgaa aaaggctggc
     5101 gtccagtcgt cggggtgcgg tctgtaggag tttgaccttg gatttcgtgc accaaaattt
     5161 ctggctgatc atgatcgtgc ttacctccgg cgtcatgctc gcatttccga acctgttcgg
     5221 caaaatcggt ggccagtccg aggtcgatgt cacacaggca gtacaactga tcaaccatga
     5281 aaacgccatg gtgctcgacg tgcgcgaaca cagtgagttc aaccagggac acattgccag
     5341 cgcgcggcat attccgctgg gccaattgcg tgacagcatg cgtaccctcg acaaattccg
     5401 cgaacagccg atcgtcgtca actgccgctc ggggcggcgc tcggcttccg cctgcgccgt
     5461 gctgcgcaag gaaggcttta cccgggtcta caacctgagc ggcggcatcc aggcatggca
     5521 gcgcgccaac atgccggtgg tcaaataatt ttcggacacg ctcatgaacg aagtcgtcat
     5581 gtactgcacc gccgtctgcc cgtattgcaa catggccgag cgcctgcttg cggggaaaca
     5641 ggtcacggtc aacaagattc gcgttgacct gaatcccgaa ctgcgcgaag aaatggtccg
     5701 ccgtaccggg cggcgtaccg taccacaaat cttcattggc gaaacccatg tcggcgggtt
     5761 cgacgatctg tccgcgctcg accgccgtgg cggtctcgac cccctgcttg ccggccgcta
     5821 atcaccgcgc gggcttgaaa ggcgtcagca cgtccccatc tggcagtcat tccagaccta
     5881 cagagtgagg caccgccatg atcgaacttg tctgtccgca ctgccaggcc atcaatcgcc
     5941 tgcccgaaca gcgtctgggc gagcggccca aatgcggcaa atgcaacaac ccgttgctga
     6001 cagctgcgcc gctcgatctc gacaacaccc gctttgaccg ctacatccag cgcaacggcc
     6061 tgccggtgct ggtcgatttc tgggccacct ggtgcggccc gtgcaaaatg atggcaccgg
     6121 cattcaagca ggccgcagca caactgagcg gccgcgtcag tttcgccaag gtagaaactg
     6181 acagcgagca gtcattggcg acgcgctacg gcatccgcag cattcccacg ctggtgctgt
     6241 tccaccaggg gcaggaaatc gcacgcatgt cgggggcgct ggatgccgcc ggcatccagc
     6301 gctggctgtc cagccagctg ccgcgagcct gatgcgcact gcccgagatc gccacagcgc
     6361 ccgcggtgca cgctataatc cgtcgatcgc gtaaaacaag gggaggcgag catggactgg
     6421 atgaaaatca tcaacgccca tgtcatgtgg aaaaaacgtc tgtccgaata cgccagaggg
     6481 gatcacacgg aacacctcga tgccagcgtc atctgccttg ataaccagtg cgagttcggc
     6541 cgctggctgt acagcaccga aggcaaggca tggcagaacg aactgccgta ccagcaggta
     6601 cgtgacctgc atgcgcagtt tcaccgcaag gcagcagaaa tcgtccagct gatcgacagg
     6661 cagcaggtgg ccgaagcctg cgaactattg cgcggcgact acagccgggt atcagagcaa
     6721 ctcaagcaca agacgctgca actctattca cagatccaca gctgagccgc gtggcgcccg
     6781 caggcgccac gcgtgccaca accgggccgg attgcccggt ccggtctcga ctcgccgatc
     6841 agggtgcagc actgtcgtgc acgaactcga tcacacgcag cgcatcgttg gcctcagcgg
     6901 caaaatcagc cgcgccgcca gtcggcgtcg gatcgaccca ggccgaaccg ctgaaaaccg
     6961 catcgtagat gttcactggc gtgggcagat agctgccatc cagctgtttc accagcacag
     7021 tgacggtggc attctcgtat gtggcggtac ggtcatattt gacggacgaa aaatcataat
     7081 agctcgttgt cacgccattg gttaccgtgt tgatccccag aatggagttg aggtacacca
     7141 ccgtatcgac cgtcagcgta ccggtcttgt cggcagcacc cgccagcagt gaggcagcag
     7201 acaggtttgc cggcagcagc gccttgacat tgtcgggcag ggtgttggtc agcaccgcct
     7261 tgtacagggc cagattttcc agtggcgaat cgatgacgct gccattgacc acgatgcggc
     7321 cggcaacgtc cagggtgatt acggcgtttg gcgccgtgat ggtggcgatg gcttcgttca
     7381 aggcatgatc cagcaccttg gccggcgcac gcgacacact cagccggctg aattcaaccg
     7441 tctgcaccag cgtcggatcg atcggattgc cttcggcatc gagcgggatc aggttgccgt
     7501 tggcatcgat cggctgcacc tgcccggcgg aattgaggat cggcacccca ttggcgtcgc
     7561 gcagaatcac ccacaagtcg ccatagaggt cgcttttctt ggttccggaa ctcgacggac
     7621 gggtccctgc gccagccttg ccgcccttca ctccggccca gatcggacgg tcgctgtctt
     7681 cacccggtcc gccctgccct ttcatcatca cgtctttctt gcctttgccg ctgccatgcg
     7741 tggtgccatg accgccggtc gaaccgtgct gcgccttgcc actgccacca ctgctgcccc
     7801 catcgccttc agcggcctgg gccgatgaaa tcagcccggc accacccgca acggcatact
     7861 gaccggcaga cacgccggcc agtgccgcga acgccgcgat gctcgcggca acgacgacac
     7921 gtttgtgaac cgaaatttgc ctagccattt tccactctcc tacccatatg acttaacaaa
     7981 aaatgacagt catcatgaaa tccggtgccg gtgacaatca cacccgccag caacatccgc
     8041 ctccgatgcc tgccgccaga acagcgccag caccactttc tccgcaacct agaaccgcag
     8101 cgccagcccc atattgaaca ggctctcaaa attcttgaat acgggtgaat cggccgatga
     8161 ctgagtggtg gtgaagccat agtcaccgta gcgcgtcata tcgtattcca gtctgacgaa
     8221 cagattgcga tatgctggca cctcggcacc aacgccaaac cggctgccat cgaccaggtc
     8281 atcgcggtct acccaggtcg gcgtactgtt gcctttgata tatgtcgtgg tgaaacgcgt
     8341 acgcacccgg cctgcacgcg catacagcag ggatccgttg ttcagcacgt aacccagtcg
     8401 cacacccgcc ccgtagcttt cctgcgcctg cacgccgaaa tcgcgcccgc cgccgccgct
     8461 ggtgctgcgg ttgtccagcc aggaggcatt gctcgattgc gcgtcgaagt cgataccgag
     8521 gtacacccga tcgagcgtga atccgtagcc ggtaaacagg ccccaggttc cggcggtttt
     8581 gccgaaatcg ccggtaaaga agcaattggc gcaccccgaa ccgccactgt cgttctgtag
     8641 cgcatccatc cgcgtcgtca gggaatccgc accggccaga gcgccgaaat agaatcccga
     8701 cacactggtg gcgtcgacga ccggcaacgg tttgcgatcg cggtcgtccg gcccgcccaa
     8761 tcgccagccc agacccagct ggaatgcgct gtcggcattg tcgaaggtat cggtagtcgt
     8821 cgcaggcgtt ccggtctgat atggggcgtg gtaactgccg taatcggtat aggcataggc
     8881 catgcgcacg aacagccgct gcgacgccgg aatgtcgacg ccaaccccgt aacgcacacc
     8941 attgaccccc tgatccagta tggcggctcc ggtcgatgcg tactgatttt ccgtttcata
     9001 ggtcttgaaa tcgctgcgca ccacgccgag gctgccatac agcagcccgc catccacggc
     9061 atagcccaga cgcagcgacg gcccataacc gcgactctcc tccagcgaca tggtgcgggc
     9121 atcggccttg tccttctggt gataccagct cgggttgcca gtaccggcat caaattccac
     9181 gccgtagtac cagcggtgcc acatccagcc gtagccaatg aacacggcgc catcgatgcc
     9241 accctgcttg gccatgtcca tttcgtccgt gccgcttccg ccatctctgg ggctatatat
     9301 ctccgacgac agcgtggtcg aactgaccag cccgcccaga tacagcccgg aataatccgg
     9361 caatgcactc gccggaatct tcagcccgcc cagatccgtc gacgccacca gcacacctga
     9421 actgtcatcg ctggacaacg ccgcagcacg cggcctgcgc ccaggcgtat aacccagact
     9481 gacgaacacc tgattacggg aaaaatcaaa cgtatcctga acgccattca ccaccaccgt
     9541 ggccgcattg gactggcgca gcaacatgcg gtaatccgcg gccagataga tttcccgggt
     9601 cacatagtat ttggcgccga aacccgcatc cagataattg tcctgccgat ccagcccctg
     9661 gaactgggcc tgcgtcatct ccaggcggcc attcacggcc aggttgcggt tgacgtcctg
     9721 atcgatccga ccgcccacga tggtatccag gtagcccgaa gcgcccggca gtgtggtctc
     9781 ttccagtgtc cggtcaacat agccggtcac ataagccgtc ggagaaatgg cgaatttggc
     9841 atcggcaccg taatagggct tgctgacgtc agcataggcc acattgtcat aattctgctg
     9901 catgtggcca aaataggctt cggccgtgtt gttgccacca aggtcgaggc caagacccgc
     9961 tgcggcacga taaccgctgg aatcgcgtcc gaccccggtc atgtcatacc gccgcgtgtc
    10021 cgtcgccaac tgggcaaaca tctggtactt cggtgcaacc tggtatgaca gccgtccgcc
    10081 gaccgaagac atgaccacat tgcggtcacc catttcgatg atgccgccgc ctggcgcagc
    10141 cacattgtca tactgcaggt gttcccaggt cgcgcccacg cgtaccgagg atttgccgaa
    10201 ctggtgaaac gctccgacat tgctcttggt atcccagaat acggttggct caaggccggt
    10261 tcgggacgga tcgacgttat accggtcctc gtggttgcgg ctgatgccgc cgccagcata
    10321 cacgttggtc gtggctgaaa tatcgtagcg cccctgaccc tcgaaccagt ggtcagaaac
    10381 gtcctcgctg ctgaatgtgt gataacggtc gacattcgca cccgtccaga aattcagact
    10441 atgacgtgcc caatcggaac gtaccgccaa ggtgggcgaa tacacggtga cccagtcccg
    10501 ggtttcattg gtccgggtgg agtagatatt cgtgtcgtac aactgactga ccgaaaggtc
    10561 ggggtaaaac agaaaggatc ccgcacgcaa tccccccggt tccggctgcg agcccggcaa
    10621 ggcagccgcc cgcatcacat ccgccggcga taacggcgaa acaggaagcg ttggattggc
    10681 ctgctggcgc aacatctcgc ccgacggcac tggctggcca ggattgagcc ccggagtata
    10741 cgtcggtggg tcggccatga ccgtctcact tgcgcaaaac aacaggctga tgactcccgc
    10801 gatcctgctt ttggagaaat tcacacgcgc ccccttgctg ccattactac atatcaatat
    10861 tgacaaatgc ttactgtaag ctttcgtaat ataccagatt ctgacttaca gcttaccaaa
    10921 tcttcacaaa attgcaacaa gaccatattg acgtaataaa tggcgaagcc aaaaaacacc
    10981 gcgcggaaaa accgtatcca tgaaatcgtt ctttaaaata cgatatgggt attgccacgg
    11041 tatggcggca acagaatgca gacgaagaat aacgaataaa aacagatcga aagggtgatt
    11101 cagccagcag ctggatgcgc cagatccagc atttcgcgcg cgtgatcgcg ggtggtggtc
    11161 gtcagggtgg caccgcccag catgcgggca atttcttcga cacgcgcgtc accatggagc
    11221 tcgacgacgt ggctgcgcac cccgtcagcg gaagaccgtt tgctgaccag caaatgctgg
    11281 ataccgtgga ccgcaacctg tggcagatga gtaatgcaca ggatttgccg ctgcgcggcc
    11341 agttgctgca acagccgacc gaccgtttcg gcaaccttgc cgccaatgcc gacgtcgacc
    11401 tcgtcaaaca ccagcactgg cacggctgcc acccggctga tggcagtctg cagcgccaga
    11461 ccgatgcgcg acagctcgcc acccgaagcc acacggctca atgggcgcaa ctggccgccg
    11521 tcatgcgccg ccacctggaa ctcgatctgt tcatggccga atgcgccgcc atcaaccgtg
    11581 gtcagcagca ccacctcgaa acgagcgccc gccagggcga gctgctgcat ggtcccggtc
    11641 acctcagcag aaagccgcgt ggcggcagcc gcacgcacgg cactgagctg ttgcgcagcc
    11701 gacgcccaac gctggcgtgc cgcctgcgcc tcggcttcga cccgtgccac atcgcccgat
    11761 ccgcccagtg cggccaaccg cgagcgcgca tcgtccagac agccggacaa ctgttccggg
    11821 cgcacccgaa acttgcgcgc cgccgtcact atctggtcca accgctgttc ggtttcggcg
    11881 agcacggccg gatcggcatc gagccggtcg gcataatgcc gcaatgcatg ctcggcttcg
    11941 tgcaattccg cacgtgccga ttcgagcagg tcgaccaccg gctgcaatgt cgcatcgagc
    12001 gccagcgccg gctccagtcc gccgagcaca gtgtcgagca gagcgagcgc cgacacctcg
    12061 gcgccgtcaa gcgcatcgat cgcggcaccg gcagcctggc ccagccgggc gacattggcc
    12121 agccgtgcat gccgtgcctg caatgcctcc cactcgccgg catcgaaacg caacgcctcg
    12181 agctcttcca cctgccagac gagctgctcg cgttcacggg caccttcgtc ctgattgcgt
    12241 cgcaacgtgg ccgcctgttc atcgagctgg cgccagcgac ggaaataccc cgccacctct
    12301 gcggcctggc ggcttgcgcc agcataggca tcgagccatt ctcgctgcat ggcctgcttg
    12361 agcagggatt gatgtgcgtg ctggccatgc agatcgacaa gaaattcgcc ggcctcgcgc
    12421 aattgttgcg cggttgcagc acgaccattg atgaaacaac gcgaacgacc gttggcgtca
    12481 acggttcgac gcaggatgca gcgctgatcg tcatcaaaat cattttcggc cagccaggcc
    12541 tgcaacgctg ccagcgctgc aatgtcaaat tcagcgctca cctcggcgcg ggacgcgcct
    12601 tcgcgcacca cggcagcgtc agcgcgttcg cccagcacca gcgacagcgc atccaccagg
    12661 atcgacttgc ctgcaccggt ctcgccagtg agcacggtca gcccggcacc gaaagcaatc
    12721 tccatctgct cgacgatgac gaaatcgcgc aatcctagcg ccagcagcat cagagtttct
    12781 ttccccagta caatttttca cgcaaggtgc ggtaatagct ataatgcagg ggatgcagca
    12841 ggcgcaccgg cgacggcgaa cgggtaatgc gcaccaggtc gccaatgcgg atgtcgcagt
    12901 ggcgctgacc gtcaaaatgc acccgggcat catccgcatg aatcagttcc agctccacca
    12961 ctgcctgact gttgaccacg atcggacgtg ccgccagcgt gtgggcgctg atcggaacca
    13021 gcacaaaggc cgccaggctc gggtgcagga tcggcccgcc tgccgacaga gcgtaagccg
    13081 tcgacccggt aggcgtggcg agaatcagac cgtccgaacg ctgtccatac acgaactcgc
    13141 catcgatccg cacgccgaat tcgatgagcc ggccggtcga tcccttgctc accaccacgt
    13201 cattgagcgc ataggcggaa aaaatcacct tgttctgccg caggatgctg gtgtcgagca
    13261 tcaggcggtc ttcctcgatg aactctccct gaaggatcag gccaagcgca tcgaacatgc
    13321 cttcgaccgg aacgtcggtg agaaagccaa gccggccctg attgacgccc agcagcggaa
    13381 caggataacc ggccagcgcg cgtgcgatcg acaacatggt gccgtcacca ccaagcacca
    13441 ccaccagatc ggcgcgctcg ccgatctcga tcagcccggc cgtggcatgg tcgccgatat
    13501 ccagcagatt ggcggtatta tgcgcaatga gcaccacata gccctgctgg cgcagaaagt
    13561 cggccagaag cggcagactg ccgcccattt ccgcgccgtc gtacttgccg accagggcaa
    13621 tcgtcttgaa gggtttgcac atgattgcgc attatatcga cgcattcccg gggcgggaat
    13681 tttttttgta caatattccc catgctgacc gaacgcgcgc aaatcctgct caaaaccctg
    13741 gtggaacgct acatcgctga tggccagccg gtcggttcgc gcatgctgtc gcagtattcc
    13801 cggctcgatc tcagtccggc gtcgatccgc aatgtcatgg ctgatctcga atcgatggga
    13861 ctggtcgcca gcccgcacca ttctgcaggc cgcgtaccca ccgctcaggg attccgcctg
    13921 ttcatcgacc gcctgatgac cgtacagcgg ctcgaatcgc tggaaatcaa ccagctcgaa
    13981 gcccacctgc aggattccga tccccagcac gtgttgtcgc agacctcgca gttgctgtcg
    14041 cagctgaccc attttgctgg cgtggtcatg attccgaaac ggcgcaacac tgcattccgc
    14101 cagatcgaat tcctgcggtt gagcgaacgc cgcatcctgt tggtgctgat caccaccgac
    14161 ggcgaggtac agaaccgcat tctcaacact gaccgcacct acagcgccgc agaactgtcg
    14221 caggccgccc aactgctcaa ccagcactat gccggcacca cgctgcgcaa ggcacgacag
    14281 accatgcaac aggaattgca gtcgctgcac cgcgacatcg accgactcat gcaacaggcg
    14341 ctcgacgcca gcaccgcggc actcgaaccg ccggcggaag actgggtgct gtcgggcgaa
    14401 cacaacctgc tcaggaccga tgccttttcc ggtatcgaac agctgcgcag gctgttcgac
    14461 ctgttcgagc agaaaacctc gctcgcgcgc ctgctcgacc tgagccagag tgccgagggt
    14521 gtacagatct tcattggcag cgaatcggaa aacgccccgc ccagcgactg cagtctggtc
    14581 tcggcaccct atcacatgaa cggggaaatc gtcggcacgc ttggcgtgat cggccccaca
    14641 cgcatgccct atgaccgcgt cgttcccatt gtggacgtca cggccaaact gctgtccaac
    14701 gcgctctcca gttaccagat caactgactg cccctatgcc catatccgct ccaccggctc
    14761 tccggccgtc cgttccccct acgacccgca ccggcatcct gctgctcaac ctgggcaccc
    14821 cggatgcacc gcgagcagat gcgctgcgac gctacctggc cgaattcctc tccgaccgcc
    14881 gggtggtgga actgccgcgc ctgctgtggt ggccgattct gtacggcatc atcctgccgt
    14941 tacgcgcccc gcaatcggca cgcaaatacg ccgccatctg ggatgcacag gacggctcgc
    15001 cactgcgctt tcacaccgaa cgtcaggcca ggctgctgga aggctggctt ggcctgcata
    15061 cccaggcggc attttctgtc gaacatgcca tgcgctacgg acagcccgac atcgccggtg
    15121 ccatcgaccg tctgctggct gccggttgca cccaactgtt ctgcctgcca ctttatccgc
    15181 aatatgcggc ctccagctcg gcgtcagcca tcgacggcgt actgacacac ctgctgcgcc
    15241 tgcgccgcca gcccaccctg cgcacgctca gcaattttca cgaccatccc ggttacattg
    15301 cagcactggc agcacagatc ggcgaatact ggcgcgcaca tggccggccg gacgtgctgg
    15361 tgatgagttt tcacggcatt ccgctgcgct cggtgaccca gggcgacccg taccacgaac
    15421 attgcctgac cagtgcccga ctgctggccg aggcactgca attgcctgaa agtggatacc
    15481 gcatcgcctt tcagtcgcgc tttggccgcg ccagatggat cgaaccgtca actgccgcca
    15541 ccctggcgga actcgggcag gcgcgcacgt ccagagtgga tgtcatctgt cccggcttcg
    15601 tgtccgactg tctcgaaacg ctggaagaaa tcgccatgga aggccggcag accttcgtgt
    15661 cagcgggcgg cggtgacttc cgctacatcc ccgccctgaa cgagcacaac gcatggatcg
    15721 agacgctggg cagaatcacg ctcgaacact gcacgccctg gctatgatct tcgcagcgtg
    15781 actcagtcgg ccacggccgg ggtcaaacca tcgcctgcag gaaaaaaccg cctggccaga
    15841 tcgtcgggcg gaaccggctg gctgaacagg tagccctgca ggtaatcgca acccatgtcg
    15901 cgcagcaatc cggcctgctc gtctgtttcc acgccttcgg ccaccacccg cagacgcagg
    15961 ctgtgcgcca gctggatggt ggcatgaatg atggcctgcg catcctcgct ggtcgcaaga
    16021 tcgcgcacga aactctggtc gatcttgagg tggtcgagcg gaaaacgtcg cagataggcc
    16081 agtgacgaat aaccggtgcc gaaatcatcg atcgacaggt tgatgccggc ctcgcgcaac
    16141 gcctgcatct tctcgacatg cgccgcgaca tcggtcatca gcatcgactc cgtgatttcc
    16201 agggtaatcc tgttgccgga gactgccaac tgatccagca caccggtaat gtgcgccacc
    16261 agctgatgat cggaaaactg ccgtgccgac aggttgaccg acacggacat ccggccctgc
    16321 cagcaggcga tctgccgcgc cacctgttcg aagatctggc gccccaatgg cacgatcaat
    16381 ccggtttctt cggcaagcgc aatgaattct gccggtggca ccaggcccag ctcgggatgg
    16441 ttccagcgtg ccagcgcctc ggccgcaatg atgtctccac ttccggcgca ggcaatcggc
    16501 tggtaatgca catgaatctg ttgctgatcc accgcctggc gcaacgcctt ttccattttc
    16561 agacggcgca ccgaggcttc gttcatgctg tgcttgaaca gccggaaggt attgcggcca
    16621 cgttccttgg cacggtacat ggcgatgtcc gcatgcttga tcagcgtatc gcggttgctg
    16681 ccgtcttccg gatagatggc gatgccgata ctgcaggtgg taacgaaagc atcctgtccg
    16741 ggcagctgga acggctgcga catgtgctcc aggacacgct gggcgatctg caccgtatca
    16801 ctggcatcca cgatttgcga gatcagcatg agaaactcgt cgccgccagt acgcgcaacc
    16861 gaatcgtctt cgcgcagact ggccttgagc cgctcggcca cctcttcgag cagccggtcg
    16921 ccgatattgt ggcccagcgt gtcgttgatg tacttgaagc gatcgagatc gacgaacatc
    16981 accgccagct tgtcctgatt gcggtgtgcg tgcgccaggg ccatgtcgag acgatcgtag
    17041 aacaggcgcc ggttgggcag gttggtcagc ggatcgtaat aagccagatg ctggatacgc
    17101 tgttcgctgg ccttgatctc ggaaatatcg gaaaacagcg ccgcatagtg aatgatctcg
    17161 cccgcgtcat cggtgatcgc ggtaatcgtc atcagttccg gataaacttc accattcttg
    17221 cgccggttcc agatctcgcc acgccagtaa ccccgttcgc gcagctcgtg ccacatggtg
    17281 gcataaaact ccggcgaatg gcgcccggag cgcaacaacg atggtttgcg gccgatcact
    17341 tccgcggcag taaaccccgt cagccgggtg aaggccggat tcacctgacg gatatatcca
    17401 tcgacgtcgg tgatgatcac cccttcgagc gacgtttcga tgatcttttc tgccagctgc
    17461 aggtttctgc gcgacagatc gagtgcctga tcgcgttcct gcaacgcact gcgcaattca
    17521 tcgacatagg ccagctcgat accggacagc aggtcggaaa agctgaccag tccaagcacc
    17581 tcgccctcac gcgtcacgcc gacgtggcgg atatggtgcc tggccatgat ttcgcgcaac
    17641 cggtacaggc tgacactcac atcgacggtg taaagatgct gcgtggccac tgtaccaaca
    17701 ctgacgtcac cagtctgttc ggccaccaga cggatcaggt cgcgttcggt aagtatgccg
    17761 tgtcggccat cgtcaaattc caccagcacg gcatcggcac gagcctgctg catttccgtt
    17821 gccaccgagc tcagacaggc acggtcactg acccggatgc cggcatgatt gagaatgctg
    17881 cccacatcgc ggaagcgcag gtagtgttcg atgccctggt tgaacaccac atccgtctgc
    17941 gtgacgatgc ccagtccacg gcccgcttca tccaccacca gcagatggcg gatgtgctcg
    18001 tgacggaaac gctgcgccac agcgctcagg cgtgcggtca cgggaatgtg tcgcaccgga
    18061 tggctcatca cgtcagcaac ggccacgccg aatgcgctac tgccggctcg aaaatcgacc
    18121 cgcagcgcat cccgctcggt ccagatgccc aacgggttgc gggtgtcgtc gacgacaatg
    18181 accgaactgc agcggcgttc gcgcatcagc cgcgcagcca catgcaccgg cgtgtgcggc
    18241 agacactcga gcagggtctg ggccggatcg agcacctccc gcaggcaggg gtcatgcgag
    18301 aaatcaccgg cagaggattg tggtcgggaa accggatcag tcaccaagct ggaaaacacc
    18361 ataccgcgcc caacaatgaa ttttccgaat attataatac ggctttgcac cgaccgtgcc
    18421 gcgcacgtcg caccggccag cgaataaacc ggccgatggc ccccgtgacg cctcagactc
    18481 tgggtgcaag caggtcacga atgcgctgca actcggccag ccgttccggt gacgaacgcg
    18541 gatattccag tttcagcgcc cgcagggttt cgagtacgac ttctgccacc agcagacgcg
    18601 cattttcctt gtcatcggcc gggatggcat accagggcgc aaactcggta ctggtggccg
    18661 acaggcaatc ttcataagcc tgcatgtagc ccttccagta gccgcgctcc tcgatgtcac
    18721 tcatgctcag cttccagttc ttgtccggat cgtcaatgcg gcgcagaaag cgtttgcgct
    18781 gttcttcctg ggacagatgc aggaagaact tgaccactcg cgtcccattg cggaacaggt
    18841 gatgttcgaa atcgacgatc gaacggaaac ggtcgcgcca gaaaacggcg cgctcgccat
    18901 tgtgttgcag tatgccctgg ttgctcagca tttccggatg tacgcgcacg atgagcactt
    18961 cctcataata cgagcgattg aaaatgccga tcagaccccg ttcaggcaat tcgcgcgttg
    19021 tacgccacag aaagtcatgt tcgagttcgg atgcgctcgg atgcttgaaa ctgtgcactt
    19081 gacagccctg tggattgacg ccggacatga catgacgaat ggtgctgtcc ttgccggcgg
    19141 catccatcgc ctgaaagatg aacagcacgg catgactcga cgcggcataa aacacttcct
    19201 gcagcgcgct cagttcggcc acctgcgctg ccagcaggtt ctgataatct gccttgctcg
    19261 cgtacagcgg tgccacgtgc gtcggctggt gagccagttc gacacgttta tccggctgaa
    19321 tgcgaaattg ttccgcgtca aacttcatgt cacttcccct ctcgtgcccg agccgtgaat
    19381 cgcttcgaat ccggccagga cgtcaaacag ctcttcgtca atacccgcct gccgctgccg
    19441 gttccattcc tggcggaact ggtgcagcag ctcgtcgatg ttcctgtcgg cgcgctgcat
    19501 ggctgcaagc cggctggcat gttcgctggc ctgcgattcg gcgcacacgc ggtagagcga
    19561 cacgaacaga tattcgcgga tcagcccgcc cagcacccgc gccggctcac cgaacacctg
    19621 tggaacccga tgtcctggcc agcgctgcgc cagcagacgc tgacgccaca cctcatcggt
    19681 gggccacagc cgggtgctgt gcggcgcata caactccccc gtacgcggac ggtggtgaaa
    19741 caccgtcact gcggcaccag gttcggcatg ctgccagggc tcaatgaccg ccagcaaccg
    19801 cgctaccagc ccggtaatgg caggaatcga gcctggcaca ctgaaatgcc cggccagcac
    19861 gatgccctcg tcctccatgc ggtcgcgcaa ccgttccccc accacccaga tgtcggcagg
    19921 cgcgcctgcg gccaactgcg ccagcgcatg gcgctgcaag tcttcattga actgtcccac
    19981 cagtccctga tcggaaccga acagaatcgc tgcctggcgc ggacgcgccg gctggtctgg
    20041 ccatccgctc tgctcacgca aggcaacgta caaccctcgc tgcaccgtct ggtcatacaa
    20101 cgccagcgct tcgactgcgc gttcgtagcg cgtaatgctg gctgccgcaa tcgccttcat
    20161 ggtccgcacc accgacttca gttcgccagc gctggacagt ttgcgttgca ggtcagagag
    20221 cgagtcgctc atgccgcggg tttccattcg tgcagcgccc gttgcgccag tgccagcagg
    20281 gcctgccgct ccgactcatc cattggcgta tcgccagcca gacgcgccag caactgcgta
    20341 tcggcccgaa ccacggcctc gcgcaccgcg gcttcggcct gcggcatgtc atcgagcggc
    20401 acgctgtcga acaatccgcc cgtgagcgcc accagcaccg ccacctgttc caggaacggc
    20461 accggcgagg attcatgttg cctgaggcag gcacgtatgc gacggccatg ttcgatgatg
    20521 cgtcggctgg tctcgtcgag ccgcgcaccg aaccgggcaa aattttccag ctcttcgaac
    20581 tgcgcatacc ccagcttgag ctcccccgcc accgcccgat acacggctcg ttgcgccttg
    20641 ccgccgacgc gcgacaccga ttttccgaca tccactgccg gcagcacgcc caattcgaac
    20701 agatcgggcg acaggtagat ctggccgtcg gtaatcgaaa tcagattggt cgggatatag
    20761 gcagaaatgt tctgagcctc ggtctcgacg atgggcagcg cggtcagcga tccgccaccc
    20821 gactccggcc gcaaccgcgt cgcacgctcc agcaggcgcg agtggatata gaaaatatcg
    20881 ccagggaacg cctcgcgccc cggcggccgg cgcaacagca gcgacagttc acggtaggcg
    20941 cgcgcatggc tggtcaggtc gtcatagacc accaggacgt cgcgcccctg tgccataaac
    21001 cattccgcaa tgctggtggc cgcatacggt gcaatgtagg acagaccggc ggcttcgttg
    21061 ccggcactcg agagcacaat gctccacgcc atggcgcccc ccgcgcgcag tgcggcaatc
    21121 acgcgggcca cgctggcggc acgctgaccg atcgcgcaat agatgcacac catattctgc
    21181 ccgcgctgac tgagcatggc atcgacggca agcgcggtct tgccggtctg ccggtcgccc
    21241 agtatcagct cgcgctggcc acgccccacc ggaatcagcg catcgaccac tttcacgccg
    21301 gtttgcagtg gttcgcttac cggagcacgc tgcatgatcg acggcgccgg tcgttcgatc
    21361 ggcaaccggg ctgaagcggc gagcggcccg agttcgtcaa gcggagcgcc aagcggatcg
    21421 atgacccgac ccagcagccc ttctcccaca ggcacgtcca tcacccgtcc ggtacggtgc
    21481 acttcgtcgc ccacctgcag tttccagtag tcgcccagca gaatgacgcc gatttcgttt
    21541 tcgtcgagat tgaaggcgat gccatacata tcgccaggaa agcgcagcag ttcctcatgg
    21601 cccgcgccag gcagcccgct cacgcgcacg atgccattgg caatgctgat cactcgaccg
    21661 gtctcgtgcc actgcagcgt ttcatcgcgg ccggactgca cctgttccat cgcatcgaac
    21721 gcctgatcga acaccgtctg caggctgtca ttcatgatgc ggtctccgca accggcatgt
    21781 tgcccgcatc gggcagcaag gccatgcgct gttcgagttc ggccagatag tcctgtgcgt
    21841 tccatgacag tcggtaaccg cctgccgcca gttccaatcc gcacaacaga tgcgggtcgg
    21901 tctcgaacac gagttcaccg gatgccggaa atgccgtgcg gatcgcctgt tcgagtgcgg
    21961 tctgctctgc ggcatccagt ggcgcagtcg agcgtacggc ccaacgttca ccgtgcccct
    22021 ggctgcccag cagcgtcagt tcgggtgccc caccctgctg cagccgcacg acgaatttcg
    22081 ctaccatcag tgtctgcagc tgttctcccg ccaggtcggc gagcaggcgg cgcgccaggc
    22141 tgtacacctc ttcacaggtg cgctggacca acgtggcgcg cagacgggaa aactcggtct
    22201 gcattgccag gtgccattcc gcctgcatgc gttcggcatc ctgccgggca tgctccagca
    22261 cgttgcggcg ttcagcctgg gcatcggcca acgtcatcgc cagcactcgt tcgcgctcgg
    22321 cctcgatttg agcctgccgt tgcgccaact gcgccgactc attctgcgcc tgctcgcgcg
    22381 cagcgcgtgc cgcggcgagc tcggcggcga tccggcgctc gcgtccggcc accgcacgca
    22441 gcaacggacg gtagaggaaa tgccgcagca gcacgatcaa cacgagaaag ttgatcgcct
    22501 gtgccagcac agtgaagcca tcgatcagca tgatcacttg ccgggcagaa cgttcagcgc
    22561 atggctccag aacggattgg caaacagcag gatcatcgac accacgaaac agtagatcgc
    22621 aatcgattcg atcatcgcca gcccgacgaa caacgtgcgc gtgatgacac cggccgcctc
    22681 aggttgctgc gcaagcgagg acagcgccga agtcacggcg cggccttcgc cgagcgcagg
    22741 tgcaatactg ccgacagcga tggtcagccc gctggtcacg atcgatgcca cagcgatcag
    22801 ggttgcatta tccataacac tttttctcct tgctgctgac ggtcaggacg gcccgcgcgc
    22861 gccatccgga tgcgctccca ccgcggctgc aatatagacc gtggcaagaa tgctgaaaat
    22921 atacgcttgc accatcccgg tgagcaagcc aagcagcttc atcaccactg ggaaaaaata
    22981 gggcgtgatc gtaagcagga tgccgagaat catgccgtca ctcatcatgt tgccgaacaa
    23041 ccgcaccgcc agtgccagcg tgcgcgtcat ctcgctgatg atattgaacg gcagcatgac
    23101 cggtgttggt tccaggtaat ccttcaggta acgccccagc ccctgttcgc ggatgccaaa
    23161 ccagggcacg gccacgaaca cacagatcgc cagcgcagcg gtggtggaca gcgaaccggt
    23221 cgggggttca taacccggaa agatcgaaca caggttggcg ctggcaacga aaatgaacag
    23281 cgtcgcaatg aaaccaagaa acttctccgt cgggtgcaac ccggtttcgc gcatctgacc
    23341 gcggatcagc tcaacgatcc attcgagcag gctgcgccag tgcgcgacac gcgcatcgtc
    23401 ctcccgcccg agcctgcgtg tcgcaagcca cgacagtccg gtcagcagcg ccatcaggcc
    23461 ccaggtcgtg acaatggtga ggttgagctt gaaccagcgg tattgccaga acacgacctg
    23521 gtcaggactc aggtgcatgc gtacctccgt cgcgcaccac cgcccgctcg cgccgggcat
    23581 gcatgacagc ggcccgctgc gccagcgcaa acccggcaat gccgcctgcc cagcgcagcg
    23641 gatcgctgcc agcgaccaga tacagcccgg ccaccacccc cagtatccgc accagcgcgc
    23701 tgccggcaaa ccacactgca ggccaggcac tgtcgaggct gcgccgtatg gtccaccaca
    23761 acccggcaaa aaacagtgcg ccgagaaagc ctccggcggc aatgctttca gccagatgca
    23821 accactcagt catctcgagt ttcctcccct ggcgaatccc cgtttttcat caccccggtc
    23881 tggacgatcc agcgccaggt attccacgca ccgaacacca gcccggccag tatcagccac
    23941 aatgtccagg aaatgccgac cggccaatgc cggtccagcc agccgccgac gatcgccccg
    24001 aacagcacgg gcaacgccat cgaccagccg accatgccca tgaaaccgag ccctgaccag
    24061 accgaccatt cctgcgaaga ctgcaatctg cgccgcgcac tgcggcgcac gtgctcgccc
    24121 agcccggacg acgggcggtg catgtcatcc atggcgcaag ttcctgaact ggcggatgaa
    24181 tccactttcc atgcgcgcca gcagcacacg cgcttcgcgc tcctcctcat ccacctgacg
    24241 caacatgtcg tcgaccgcct gctgcatcgc tgccaggtca ttacccgata acgcatgccg
    24301 cgccgacacc tgcacgtctt cgccggcctt caccagcacg ccctcatcca cggccacata
    24361 gtgccgctgc gtggcgtccg tcgcatagct caggatgccc ggcaccagca gcgccacgca
    24421 gtcgagccgc tgtggcagga tgcccaggct gccgtccacg gtttcggcca cgaccgtacg
    24481 cacgccatcc acatcgagca acaccgcctc gggcagtgaa acgcgcagtc tcatgcggcc
    24541 tgctccgggc gccgggcttc gtcgatcgaa ccgatcatgt agagcgcgct ttccggataa
    24601 tcgcggaatt catcatgcag gatacgttcg caaccatcga gcgcatcttc caggcttacc
    24661 agtttgcccg gcatgccgct gaactggccg gtggtgaaaa atggctgggt gagaaaatgt
    24721 tccagccgcc gggcccgatt gaccacccgc cggtcctccg gcgacaactg ttcgagcccg
    24781 agcatggcga tgatgtcctt cagcccctca tactgcgcca gcgtacgacg gatctcctgt
    24841 gccaaccggt aatggcgctc gccaaccacg cccgggttgg ccagccgcga attcgattgc
    24901 agcgggtcaa ccgccggata caatccctcg ctggcacgct tgcgcgacag cacgatggac
    24961 gccgacaggt gagaaaaggt atgcactgcg gccgggtcgg taaaatcgtc cgccggcaca
    25021 tagactgcct ggatggaggt gattgcacca gattccgtgt cggcgatgcg ttcctccagt
    25081 tcggccagct ccgtgcccag tgtaggctgg taaccgagac gcgcaggcat ctgtcccatc
    25141 aggccggata cctcggcacc ggcctggaca aaacggaaga tgttgtcaat gagcagcagc
    25201 acgtcacggt gctcgtcgtc acggaaatac tccgccatgg tcagggcggc atggccgatg
    25261 cgaaaccggc tgcccggcgg ttcattcatc tggccgtaga gcatcaccat gttgggcagg
    25321 accccggcct gctgcatgtc acggtacagc tcctcgcctt cacgacaacg ttcaccgatg
    25381 ccgcagaaaa tgctcacccc ctgatgctga ccgaccatgt tgtggatcat ttccgtcagc
    25441 aggacggtct ttcccacgcc ggcacccccg aacaggccgg ttttgccgcc ccgttccagc
    25501 ggagtgagta catcgatgat cttgatgccg gtagcgaaca ccgtcgattg gctcgtgcgc
    25561 tgcgacagca agggtggtgc acggtgcacc gaacgccatt ggacatcctg cagcggcgcc
    25621 ccctgatcgg cgggctggcc gaacacatcg aacatgcgcc ccagcactgc ccggcctacc
    25681 ggtgcctgca gcggcccacc ggtatcgagc accatcgcgc cgcgcgacag accctgcacc
    25741 ggcgtgagtg caatgccacg cacatgctgc gcatccagtt gcaccatgac ttcaatcgcc
    25801 atcccgcgat cgtcgccggc acgcaacagc gtgaatacag ctggcaggcg ttgcgggaac
    25861 cgcacgtcga tcacgctgcc acgcactgca atcaccgtcc cgagattttc ttgcgtcatg
    25921 gtctgtccgc cgaaatgtcg ctccatggac cgttcaccgg tcaaccggat aacgttgcct
    25981 caggcaacgt ccgtgctcgc cgtgtcgcgc gccaaagcaa actgcaggaa ctccgcttcg
    26041 tctagtggct tcgcatacag gtaaccctgg ccgaaatcgc aatcaaagtg cttgagccgt
    26101 tcatgctgct cgcgcgtttc aaccccttcc gcaatgacct tgagtcccag cttgtgcgcc
    26161 atgaccacga tggcctcaac cagcgcggcg gcactttcgt ccacgaccat gttgcgcacg
    26221 aacgactggt cgatcttgag aaaatcaatg tcaaaccggc gcagatagga caacgccgaa
    26281 tagccagtgc caaaatcatc cagggccacc tggacacctg catttcgaaa atcgagcagc
    26341 ttggcaaaga tctgctcact tgcatccatc agcaaccctt cggtaatctc gaccgccaca
    26401 tggcgggtgg acagaccgat cgcctgcaga tgcgcaagcc aggacgcaat gtgcacggca
    26461 tcatccctga actgtaccgg cgacatgttc acgctgacct ggatttccgt gtcgaggtgc
    26521 accatgaccc ggcgcaccca ctctgcggcc tgatggaaca cccagtcccc aatcggtacg
    26581 atcagacccg attcctccgc cagcggaatg aacgtgccag gatcgattct gccgcgcgtc
    26641 gggtgctgcc agcgcagcaa cgcctctgcc ttgaccacgc ggccgctctc catgtccacc
    26701 accggctgga actggaggaa aaactgcccc tgctccatcg caccacgcat gtcatgcaac
    26761 agttgcaacc gatgctgcac cttctcctgc atgcctgagg cataaaaatt cacgcgattg
    26821 cgccccgtct ccttggacac gtgcatggcc tgttccgctt tttgcagcaa gtcctcggtc
    26881 ccactggcat cgagcggata caaggcgaca ccgatcgagg cggaaatgta tgtcttgcgc
    26941 cgatcggggc ccaactccac tggcgaagcc agttgcgcca acacacattg ggcaactgcg
    27001 gcagcatgcg acagcgtggc attccccagc agcagaatgg caaactcgtc gccgttgagc
    27061 cggcaggtca ccgatgcgtc acccgcgcag gctgctattc gcgcggccac gtccaccagc
    27121 aacgcgtcgc cggcctggtg gcccaggata tcgttgatct gcttgaactg atcgagatcg
    27181 atgtgcagca ccgcaagcga cacctgctcg ccacgagcgg tacgcgcgat ttcggcttcc
    27241 agctgacgcc aggccatgta acggttgggc agactggtga gcaggtcgaa attggcctga
    27301 cgccagatcg tctcttcgaa tttcaccttg tcggaaatgt ccgaggacgt ggcgacatac
    27361 cgatcgggca agccgtcttc gccgagcacc acgttgatgg tcatccattc cgggtacacc
    27421 tcaccgttct tgcgccggtt ccatacccgg ccctgccaga agcgctgctg ctccagatcc
    27481 tgccacatgc cggcataaaa ggcggcatcg tgacaacccg aattgaatac ccgcggattg
    27541 cgcccacgca actcatccag cgcatacccc gtcatctgct cataggcatg gtttaccgaa
    27601 atgatgtcgt tattgcggtc agcgaccagc atgggctggc tgctgtgctc aaacaccagc
    27661 gcggccattt ttacgcgatc ctcaagctgc tgccgcgcac gctcctgtcc cagatgatcg
    27721 agcgcaaacg aaatggcctg tgtcagcgcc tcgatcagtt gcaccatctc gacgtcgaat
    27781 acccccggtt cggtgtgata caacccgaga atcaccgcca cccgaccctc acggaaaatc
    27841 gggaacgaac ccgaagaccg ccagcccgtc agcctcgcct gctgacgcca cgacagtgtg
    27901 gctggatcgt tgaggtagtc gttgacgata taccctcgcc cgctgcgata cgcattgccg
    27961 gtcgctcccc atccatgcgg cgaatcggcc agcaccgaaa tcttcacctg atcgagaaac
    28021 ccggtgccga caccaaacct ggccagcgga cgaaccacac cggaatgttc gtccggctga
    28081 ccgatccacg ccagcgtcat gccccccagc tgcaccgcag tgcggcagat ggcctcatac
    28141 agcggcaact cgtcgcggac ctcgatcacg gcgcggtgaa tggccagcaa tgcctggtag
    28201 aaacgcacca gccggttgac gcgcgcatga aacttgcggg gcagacgcag atcgccggcc
    28261 atgagcaaca gacaacgcgt ctgcccctgc acgatcggct ggaaagtcac tgcaaccgga
    28321 agatcactgc catccgcagc gcgcagcgat acctcggtgt tgagcagggc ctgtccgtgt
    28381 tcgtccacgg acaagagcag ttcgcgccag ggcgcgatat cgccatcagg cgacagcacg
    28441 gataccggct gtccgtacag cgcctcacgg cccagcccgg tggcggcaat cagcgcggca
    28501 ttggcatagc agatcgacag cccctcattt tcgagtacac agaccatttc ctgcaatgca
    28561 tcgagcatgg acagaaaaat ctgcgcttca tcccgctgca tgaaagaaca gctcgacatg
    28621 accggctccg cgtgccgcgc agcggacatc cgccgcgcat cactttcatg ctcgcatatt
    28681 tttatggggc aatcaaacgc gccaagctag ccgcgcacgc gaaacacgtc gatcacctgc
    28741 gacagcttgg ccgcctgctg ctgcaatgct tccacccgtg ccgcctgttc ctgggcattg
    28801 cctccgactg cagccgacag actggatatg ttggccacca gcgcatcggt atccgacacg
    28861 acagccacca ggttgctctt catctgattc agcgccagca tcagttcggc cgtctcatcc
    28921 gccgcgtcca tctcgaagcg caacgacagg tcgccccacg aaatggcatg cgccacttcc
    28981 agcgcatgat cgataggcag cagaatcgtg cgcgaaatga aatatccaaa cagtaacagc
    29041 aacaccccgc ccagagctcc ggccgctgca agcgacaacg gcagccagct gtccgcacct
    29101 gtcgaatgtg cacccgccga ggtctcccac cagcccagcg cccccagcgt ccacatcagt
    29161 acggccgcag ccgcagtcgt caactggata cgcaaccgga ccggaatgcg gcgaatcgaa
    29221 tccatgatcc cggccagacc tccccgctcg atgcgcccgt gacgaatgcg catgttgccc
    29281 tgccgacctt cgcgaaacag gcgatagacc cgctcggcct cggccacctg ctgcgccgag
    29341 ggtgtgttgc tgaccgcaag gaaacctgag gtgggcggct tcttctgtag tggcgtgaca
    29401 ttcatcaaca cccagaaata atccccgttc ttgcgccggt tctttaccat gccggtccag
    29461 ggcaggcgtt cgctcagcgt cacccacaaa tcggaaaaca cctcggccgg catgtccgga
    29521 tgacgcacaa cgttatgcgc gtcacccagc aattcctgct cgctaaaacc gctcgcttcg
    29581 atgaacgccg gattggcata ggtgatcttt cccttgagat cagtcttggt caccatggac
    29641 acgccatcgt caagccgata ctcttgctca gtcacaggca aattggaacg catgcactcc
    29701 ccctgatgaa tcatcgcaaa aaaacgcctg ataatcatac cgcaaaacaa tctacaagat
    29761 aatggaatca ttaaatcttt tataatccgg caccctgccg gacgacctga agcctggact
    29821 ccaggtagcc gtgcagccga tcaggcccgc aatttctgca tggccaccgc tgcagcagac
    29881 gtgcgggttt ctacgcccag cttttcaaaa atatgctcaa gatgtttgtt gacggtacgg
    29941 tggctgctgc caagaatgtc accgatgtcg cggttggttt ttccataaat cagccaatac
    30001 agcacttcgg cttcgcgcgc agtcaaatga aaacccgtgg ccaggtgctg cagcgccaca
    30061 gtacggtttt cttcacgtaa cgtgatcagc caggagtgtt catggcccga atcacgcagg
    30121 ctgcccaata atcgctgccc ctcctgttcg gccatgacag gcacttcgcg atgttcctgc
    30181 atggcttgcg atgctttgtc gatccagtcc atcaggcagc cctgggcaga gtcattgctg
    30241 cagccgaaat acttgtccag caggctgcgc gcctgaggcg tttgccagga cacctgacca
    30301 tccgatatgc gcaccgaaag cgtcgcctgc ccaaaggtgt ccagcgcgct gcgcgcctgc
    30361 ctgatctgtc gtgcattctg catatgcgct gcaatgcgcg ccaacacctc tcgtggccgg
    30421 atcggcttgg tcacataatc tgccccgccg ctggcaaaag ccgcgaccac actttctgtt
    30481 tcgcttagcc cggtcatgaa aataatggga atatgctgcg tacagaattc agctttcaga
    30541 ccgtgcgctg cagcataacc gtccattccg ggcatgagca catccagcag gatcacgtct
    30601 ggcagaccgg tgcgcgcaat ctgcaaggcg ctttcgccat tggtggcaac caataccctg
    30661 tagccgacat cgtccagcgc atcatgcagc agactcaggt tttccggcac atcatccaca
    30721 atcagcaccg tgccttcctg caccgtctca tgcatgatca tgatccacct ccgacatccc
    30781 cttgtgcaaa atcgatttca tcattccaaa ctggaactgc agtgcgagtt ggcgcatatt
    30841 gtctacaaaa tccacgtaga tactgtccat ttggcgaatt tcatccagct tgcccaatat
    30901 tccgcgcaca tagccgtgtt cgacatgttc atgcagatca tgcaaatgca tcattgacgg
    30961 ataacaggtt cttgccggga tgcttgaaac gtttctgact ggggcgggcg tttcaataca
    31021 cacccattgc aacgcaagat gccgcccgat ccagtcgagc agatcattca cattgaccgg
    31081 tttggtaata aagttttcgc tggagatgcc cgcgtcatta tctgcatttt tttcgaaggc
    31141 attggcagaa atgacggcga tttcacattc ggtcagacga tcacggcgga tacgccggat
    31201 cacctcccag ccgtcgatcc ccggcatggc cagatccatg aaaatcaggt gcggacaaaa
    31261 tacgggaatc aaatccaggc attcctgtcc ggagccggca gcagcgatgt caaatccgat
    31321 gggagcgagc atgctcgaca acacgtcacg gtcggttttt tcattgtcga caatcaggat
    31381 tctctggcgc acgccaaaat aaccgacccg ctgtgcacgc acaacttcct gttgtggctg
    31441 ggccaaccgg atatgtggca gaaacagacg aatgcggaaa cggcaacctt ttcccggctc
    31501 gctgtccacg gtcaattcac cgcccatcag atcggtcagc atcttggcag tggtcagccc
    31561 ggggcccgta ccgccagtcg caccgatctg cgccgctgcg ccgcgcgcga atggttcgaa
    31621 cacatgctca atctcgtccg acgcaatgcc gggaccagta tcctcaatac tgaatgttgc
    31681 aatttcgccc tggtacctga tccggaacgt caccatcccc tgtgccgtga acttgacggc
    31741 attgcccagg atattgatca atatctggcg cagacggctg gcatcggcgc gcaccagctc
    31801 aggcagttcg ccttcgggac aatacttgaa cgtaatgccc ttgcttcgcg cctgcaactc
    31861 gaacatgttg acgatctgca gcacgaaatc cggaaactgc agcggcttga cgttcagttg
    31921 caccttgccg ctctcaatac gagcaatatc cagcgtaccc tcgatcagcg acagcaaatg
    31981 gtcgccgctg cgccggatca caccgatggc ctgccgtcgg ttggacggga tggtctcatc
    32041 accgtcgagg atctgcgcat agcccagaat gctgttgagc ggtgtacgca attcgtgact
    32101 gatcgacatg atgtagcgac ttttggcctg attggccaga tcggccgtct gtttcgcctg
    32161 ttgcaacaac tgatccgtcc gatggtgtga gttgatctcc tgcaccagca gctgggtctg
    32221 ataattggct tcctgctggg ccaccttgcg acttttgctg gtcaacacca tccaccaggc
    32281 gatgataccg gtcaacagga tcagcaccgc tgaaatacgc acgaaaacac cctgcaactg
    32341 gggcagcaac ccttcattca ccgcaccgac agacaggatt tcctgaaaat acagcaaccc
    32401 gatcagcaca cccaacagac tgatgaaccc tgtcatcaac aacaggtaat gcgccagtcc
    32461 ggcttccaga tacgggcgta gcgccacagg aagaaaacga cccagcacat cagcccattg
    32521 ctgagccaac cgggcttccg gtttgcacaa atcattgcaa cgcgcatcca gcgtgcagca
    32581 cagagaacag atattgccct ggtatgcagg acagaccgac acgtcttccg tttcgaaacg
    32641 ggtaccgcag atgcaacaga catgccctgc ggcagacggg tcggcaaccg gtgcttcaac
    32701 cggacgggca atgtaatagc gcccatgggt cagccatgca aacagggggg acaggataaa
    32761 tggcaacccg atggcgataa aaatcgcata cggctgcgct gtggggccaa gcaacccaag
    32821 atacaccaca atcgacacca ccgacgcaat gcccattgca ccaaatccaa ccggattgat
    32881 gtcatacaga taggcacgac ggaactcgat acccgccggc gacaggccaa accgtttatt
    32941 gatcaccaga tccgcaacga cagccatcat ccaggccacc gcaacattgg agtagagccc
    33001 cagcacccga ctcatggcct ccagcagccc caattccatg agcatgaagg cgatcaacgt
    33061 attgaatacc acccacacca cccgtcccgg atggctgtga gtcagtcggg agaaaaaatt
    33121 ggaccaggcc aacgagccgg catacgcatt ggttacattg atttttagct gtgaaatgat
    33181 gacaaacacg aatgtagccg ccacagccca gtccaggtcg tggaagacat aaccccaggc
    33241 aatcagatac atctgattcg ggttcaccgc cagcgcttcc ggaacggcat gccggatggc
    33301 cagataagcc agcaatgcac ctcccagcat cttgatggca gaaagaagca gccagcctgt
    33361 tccgccgaac agcaccccgc catgccagtt gcgccagttg cctgcagtac gttccggcat
    33421 gaagcgcaga taatccgcct gttcgcccat ctgggcaata atggccatgg caacagtcat
    33481 tgccgccgca aacagcctca tgttgaatcc gctcgcgccg tcatgtccgc cgtaggagaa
    33541 caatccggca acctcctgcg gactgtgcaa caacaggaaa atgtagggca gaaacagcat
    33601 gaccagccag accggctggg tcaatgcctg aaaacggctg atgacggtaa taccccgcgt
    33661 gacgatcggg atcaccacca gggcacagag cagatagccc caggccagag gaatgccaaa
    33721 caccagtttg agcgctgaag ccatgatggc ggcttccagc gcaaaaaata tgaacgtgaa
    33781 tgccgcatag atcagggacg tgaaggtcga gcccagataa ccgaagcccg atccgcgtgt
    33841 cagcaagtcc atgtccaggc catacaacgc cgaatatcgg ctcaatggca ggctgaccat
    33901 gaaaatgatc agcccggcag cgaaaatggc cagggcggca ttacagaatc cataattgac
    33961 cagcaacgtg gcaccgatcg cctccagcac caggaaagag gacgagctga atgccgtatt
    34021 ggccacctgc cacacaggcc acttacggta actgcgcggc gtgaaacgca atgcataatc
    34081 ttccagcgtt tcatttgcca cccaggtgtt gtaatcgcgc cgcaccttga atacattctg
    34141 cacggcattg tcgctcctga tcggatcaag cctgtcggca ttcatgggct ttcgcccgcc
    34201 ggtacacgcg taaaacacga ctcgcccccg tgactcatcc ggaagcgccg aaaacatttc
    34261 gcgctacccg tcctgatcac aagcatactt cgcgccagat ccgatcctac cctgtcacgc
    34321 cgaccggtca ccggtgatgc gtccgttccg gatgcggtat cccggggaca gggcactctt
    34381 ccgtgccgac catcggccgc gtcagcgctt ccaccatctg ctgcgtaccg tgcggatcac
    34441 tgatccagtg ttcgtaaaaa ccgtaagggc aagccgccat ccccttgtca ccatcgcccg
    34501 aattgatggt gccggtatac ccgcgatgca ccagtttgaa cagatggttc tcggattgcc
    34561 cgttacgacg aaaatcgcga atcaggttct tggacagcgc ggcgtactgg atgcccatct
    34621 cttcttcacc gcattcgccg agggtacggc catcgaatcc gatcagtgcc gaatggccaa
    34681 aataggaata caccccgtcg aatccggctg cattggctac cgccacataa gtattgttgg
    34741 cgaacgccat ggccttggac atcagcacct gctgttcttt ggccgggtac atgtacccct
    34801 gacagcgtac aatcagctcc gcacccttca tcgcgcaatc gcgccagatt tccggatagt
    34861 taccatcgtc gcaaatgatc aaactgattt tcagcccctt cggcccatcc gagacgtacg
    34921 tggaattgcc cggataccag ccttcgatcg gtacccaggg catgatcttg cgatatttct
    34981 ggacgatctc gccatggtcg ttcatgagaa tgagcgtgtt gtacggcgcc ttgtgcggat
    35041 gctcctcgtg ccgttcgccg gtcagcgaga acacacccca tactttggcg ttacggcagg
    35101 cagcggcaaa tatttccgtc tcggcccctg gcaccgtggt agccgtttca tacatttcct
    35161 cgcggtcata catgatgccg tgagtgctgt attcggggaa aatcaccaga tccagccctg
    35221 gcaaccccga tttcatgcct tcaaccatgc gcgcaatggc tcgcgcgttc tcaagcactt
    35281 cggcccgact gtgcaaacgc ggcatcctgt aattgaccac cgccactccg accgtatcat
    35341 tgctgctgga aatatcgcca tgaatcatgt taaccctctc gtaagcgtga cagattgcgt
    35401 ccggttcaat gactgatcat ccacggtcgc ctgtcggaaa aagatttggc agccaccgac
    35461 ccgccactcc cggccttgcc gcagccacaa ccggaatggt gccggtgtgt tgccgacagt
    35521 ttgggctcat gacgtgcgcg ttcattggtc gccatggcaa aacgcgcagc agcgggcatg
    35581 tcggcaagcc cgggcgcatg caccagaact cgatcggcca tcatgccgca ctgcggacaa
    35641 ctcccgggct cgtcacgttc cgccatacgg cgcatggcct cgaatggtcc gcaggtaaca
    35701 cagcgataat cgtaaatcgg catgatttat ccccgatctg gcgacatggg aatatcaacg
    35761 ccatcgatgt gtttcaccgg tcc
//
LOCUS       DAIANL010000154         8095 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3048321, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000154 DAIANL010000000
VERSION     DAIANL010000154.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8095)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3048321"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1818 a         2342 c         2119 g         1816 t
ORIGIN      
        1 tgcgcgaatt gtcccgtatt acccatcgca atggctgcgg catcctgatg gcacccatct
       61 gtcttgacat cgacgacaca ctggaagatc cttctgtcac cagtgaggcg gaacgctggc
      121 gcctgttcgg ccagaatgac catgtgcgac tgtactcaca ccaggggtat gtcgatacgc
      181 tcgaacataa cggtttttgc gtagaacaag taggaatcga tgctattggt catgaggcat
      241 ctgcacggat gggtctgtcg ccgaccagca tcctgtatat cgttaccaaa gcaccgcaag
      301 ccgacagcac acacaactcc gcaagcgaac atacctgacc atgagcactg aaaaacccat
      361 ttatgtcacc cagccgtttt tgccgccgct ggaagaattt gcgccttacc tggaaaaaat
      421 ctggaacagc cggatcctcg ccaacaatgg cccattccat cgtgaactgg agcaggccct
      481 gtgtcagtat ctcggcgtcg agcatatctc gttgtttgcc aatggtacgc tggcactggt
      541 cacagcgctg caggcgctgc gcatcactgg tgaagtcatc accaccccgt actcgttcgt
      601 ggccacatcg cattcactgt tgtggaatgg cgtcaaaccg gtattcgtcg atattgatcc
      661 cgacaacttc aacattgatc ccgccaggat cgaagccgcc attacgccgc aaaccactgc
      721 aatcatgccg gtgcactgtt atggccgccc atgcgatgtg cacgccattc agcatatcgc
      781 agacaactac aacctcaagg taatctatga tgccgcacac gcgttcggtg tcaacgatgc
      841 cggtggcacc gtgttgcgtc atggcgacct gtcggtgctg agctttcatg ccaccaaagt
      901 gtttaacact tttgagggcg gggccatcat ctgcccggat gccaaaacaa aaacccgcat
      961 agatcacctc aagaacttcg gctatgttga tgaattaacg gttgttgccc ccgggttaaa
     1021 cggcaagatg aatgagttta acgccgcact cggtctgctg cagctaaaat acatcgacca
     1081 ggcgattgcc gaacgccagt gcatcgacgc ggcttaccgc gaacaactca ccggcatttc
     1141 cggcatccgt tttctgcctg cagcggaagt gcgcagtgcc aattatgcct attttcccat
     1201 tctcgttgat gcggcgtatc cgctgtcacg cgatacgctg aatgaccgac tcaaggaaca
     1261 caacatctac gcccgacgct atttttatcc gctgatctcg gatttcccca tgtatcgcgg
     1321 actaccatcg gcaaaacagg agaatttacc agtggcgaca gaaatggcct cgcaggtatt
     1381 gtgcctaccc atctatcccg gactggacga aacgggacag caacgcatta cccggttgat
     1441 ccgttccctc gctgatacga agcacgccac ttgacactcg gagccaagtt ggacaacccg
     1501 acacaagcca ttggcgggta tttcggcctc gacttgccgg attacggtga actttaccca
     1561 caggccgtac gttaccagtc ggcgcgctcg gcaatccgcg ctgtgctcga atgcaacggc
     1621 atccgacgcg catggatacc ggcttacgtt tgcgattcgg tactgcgcgc ggcacaggaa
     1681 gcagaagttg aaaccagcac ctacgcgctc gacgagaacc tcgatccgca acaattgcca
     1741 tacacactgg cggatgatga ggtggtgctt tatgtcaatt ttttcggctt gtgccaatca
     1801 gcggtcactc gcctgcaaaa ccgggttgca ccggaacagc tcatcatcga caacagccac
     1861 gccctttttg ctccgcatga caatgtgctg gcgacaattt attcaccacg gaaatttgcc
     1921 ggtctgccag acggcggttt gttgctgcac ttaccggcgc tggacatcac cgcccctgcc
     1981 gtcgaggata ccggctcatt cgagcgcatg aaattcctgc tgttgcgcat ggcctgttcc
     2041 gcccgtgagg gctatgccgc attcaatgaa gcgcgcaact cactcaatgg tctgccgcca
     2101 cttgccatgt cgcgcctgac ccggcgactg atgcgcagca tcgattggca acaggtcagg
     2161 acgcgccggc atgacaattt tgccgtgatg acgcgcttgc tgggcgaact gaacggtttg
     2221 cactgggcgc cgactgacgc cgacgttcca ttgtgctatc ctctgaccct gaaaggaaga
     2281 gacgtgtccg cgctcaaggc cagcctcgcc gcagaggata tttttaccgc tacctactgg
     2341 cctgatgcat tgccccggat tcatccggga agcatcgaag ccacgctggt gcacgacaca
     2401 ctgtttttgc ccgtcgatca gcgcctcgat ttggcgcaaa ctgaatcaac cgccaggcgc
     2461 gtcctgcaac tgctgtccca gaccagacgc tgaaaggatt gattgataat gagtgataca
     2521 caaatgagtg atacacagcc ttacgcaaag ttcagttatg acgcacatgc ccgaacctgc
     2581 gccccggatg attttctcgg acaaacgcgc cgcaccgtgc aaggcgtgcc aatccctgat
     2641 gcacagatcc acatgattct ggatgcgatg cgccatggac tgcaactgcg tgaagatgac
     2701 gtgctgctcg aactggcctg cggcaatggc gcgctttcgg tccagatgtt cgatgcctgc
     2761 caagggtatc tgggcaccga catctcggaa tacctgatat ccgtggccaa gcagcatttc
     2821 gaacgccagc ccgattaccg cttcgaaaaa cagggggcta tcgaatgtgt gcaccacgaa
     2881 ccgcaaccgg aacggtttac ccgagcgttg tgctatgccg gattccagta tttttccgat
     2941 cccgatgccg cagacatcct tttaacgctg cacgaccatt tcacgcaact gcaacgcatg
     3001 ttcatcggca acctgccgga ccgggctcgc gccgccgaat tctataccag ccgtgaagtc
     3061 agtgcggcag aactatccga ccctgataca gcgctgggca tctggcgcac tcgggatgcc
     3121 tttgcacaac tggcgcacga taccggatgg caggtggaat tttccttcat gccgccggga
     3181 ttccatgccg cgcattaccg ctatgacgcc gtactgacac gcaggacctg aattccgtgg
     3241 ctggcaaagt acatattgat gacttggctc tgttcggtgg cagcccgacc tttgccgtcg
     3301 ccaaatccac ctccaacctg ctgcaacccg atttcgcccg gttcatgaca tattcagacc
     3361 tgtttctgca ggcacggcaa tacatcgaca gcggtcctgc ggtgcaactg ctcgaacgcc
     3421 gtctggccga atttcatcag gcagcgcatt gcatcacgtt ttgcagcggt ttctgggcgc
     3481 tggcgctaac cattgccgcg ttggctctgc cgggaaagcg tgaaatcatt atgccgtcac
     3541 tcacctatcg ccgcatgcct gacatcgccg cctggctcgg gctcatcccc cgattctgcg
     3601 aggttgaaac ggatacgctg gcggtaagtg ctgcaaccgt ggccacacag atcagtaacg
     3661 ataccgcact aatccttgca ccgcacccta ttgtcaactg ctgcgacgca gcaggactgg
     3721 tacagcttgc gcacgaacgc agtttgccgc tgctgtttga ttcagttgaa tcggtgtacg
     3781 aatccatgcc ggaaggcagg gtgggtagca tcggtcgcgc tgaagctttc tcgttacatg
     3841 catgcaaact gattaatggc ttcggtggcg gctatatcac caccagtgac acaggtctgg
     3901 tgcggcaact cgaagcgcta cgccattacg gcatgaacga acgcggcgaa gtgatcttgc
     3961 cgcacggcat gaatgccagt ctgaacgaaa tgcatgccgc catggcgctg gcaagcctgg
     4021 acgatgtcga acagcaggtg cagcgtaatc gcgaacgcta ccacacctac cggcgcagac
     4081 tggccggact gccgggaatc cgcctgctcg gtttcgacga acgccacgca actggctaca
     4141 agaatatcgt ggtggaattg ctcgacagtt ggcccttgtc acgcgagcag accatcttca
     4201 tcttgaatgc agagaagatt ttcgcccggg tctattattc accgccactg caccgcaaga
     4261 tgatggatcc cgccggcatc cctgtgcagc tacctgtgac tgagcagcta gccgaacgtt
     4321 tcgtgaatct gccttgtggc catctcgtca gtccccagga tatcgaacaa atcgttgata
     4381 ttctcgtttt catcgccgca aaggcgcaac ccatccgggt gcgactgcag gagctgatgc
     4441 aggagcaggg tatatgagcg ccaatatgtt tgccctgacc ggcgcggcac cattgttcga
     4501 gcgcccgctg cgctgcgggc aaaattactt tccttcgcgc gaacgctatg aaaccgcatt
     4561 tcgcgggatt ttcacgcggc aatattacaa caacaacgga ccgttacacg aacagctcga
     4621 gacccgcctg caggaattcc tcggcgtccg ccacgtgatc tgcaccatca acgccatgct
     4681 tggattgatg atcgcggccg atgcgctgca actgcgcggc aaagtcatcg tgtcggcctt
     4741 cgcgcacccc ggcaccatcc agtcgctcag atgggctgga cttgaagcag tatggtgtga
     4801 tgtcaatgca caaacccacc agatcgacgt cgaacatgca tctgcactca tcgatagcga
     4861 cactgctgca attctggcag tcaatgcctg gggtggcgca tgcgatgccg gccgcctgcg
     4921 acaaatcgcc gacgcacatg gcatccgcct gtatttcgat tcggcacacg cctttggctg
     4981 cacaaccccg gccggccgca ttggcaattt cggccatgcc gagatctttt ccctgcatgc
     5041 agatatgatt ctcggcgctg gcgatggtgg ctgcattgcc actaacgatg acagtatcgc
     5101 gctgcgcgcg cgtagcatcc gcccgagtta cgggttggcg cagccagcga gtatcgcgcg
     5161 cgtagccaat gcgcgcctgt cggaagcaca ggccgccatc gccctcatga atctcgacga
     5221 tttcttcgct catcaggcgc acaatcgtac gctgcgtacg gcttatgcct acgggttagc
     5281 cgacatcccc ggcctgacgc tgcacgagcc gaccggaatc agcgaatcga actggcagca
     5341 catggtggtg accatcgatg aatcagccta tggtatgtca cgtgaccgcc tcatcgaatt
     5401 tctggtggca gaaaatgtgt gtgttacgcg tggctttgcc gatggcattc atctcgacgg
     5461 cgcgagtaac ttgccaaacg cctcgcgtct gtacgcaacc tgtctggaat tgccgctcgg
     5521 tgcacaagtc acggtagata cggttgagcg catctgcgaa ttgctgttat gtatccagcg
     5581 ctttgccgtc actcatcgga agcatcatgg ccaataaacc tatgttcggc attatgcagc
     5641 catatttctt tccttacctg gggcattttt cgctgatagc cgccgtggac gaatggctgg
     5701 tattcgacat tacgcaatat acgccaaaaa cctggatgaa tcgcaatcgc atcctgcatc
     5761 cgggcgaggg ttggcagtat gtcagcgtac cgcttgccaa cggttcaata tccatcaaaa
     5821 caagcgaggc gcgaatgctt gatccggctg gggtacgcaa gagtatggcg ggcaagctga
     5881 gccattacaa gcgcaaggcg ccatattatg atgccgtatt acaacttgtc gaagaaactt
     5941 ttgacgacca cgatgattcg ctggtacggt tggatatccg tggccttgcc gtagtctgcc
     6001 gctatctcga tatcccgttc cgccaccgca tctgctcgga actggatctt gacttgcctg
     6061 acaacctggg accgggcgaa tgggcactcg aaatcagcag acagttcggc gccggcagtt
     6121 acctcaatcc ggcaagcgga caggcattgt tcgacccggc tcgcttcgcc gagcgcggaa
     6181 tcgaactcta tttcgcccgg cccagcgaat tttcctatca taccggacat tatcgctatg
     6241 agccttggct gtcgattctt gacgtactga tgtggaatcc gcccgatgtc gtcgcccgtg
     6301 ccgcgcgcga gcacgtcgtc attgaaccgg cccgccacac tgaatgaaag caacaggagc
     6361 gcaatccgtc atgccaaaaa tatcggtcat cctgacctcg ttcaatcacg aaaaatacat
     6421 cgccgagacc atcgacagcg tactcgcaca gactttcacc gatttcgaac tcatcatctg
     6481 ggatgacttg tcagcagatc gttcttggga cattatccaa agctatcggg atccacgcat
     6541 ccgtgtgttt cgcaatgaag aacgccgtcg cggtttttat ggcatcaacc gagccatcac
     6601 cgaagtggcc agcggtaaat acattgccat tcaccattcc gacgatgtat gggaaccgga
     6661 caaacttgaa ctgcagcacg cgtttcttga atcacatccc cacatcggcg cggtattttc
     6721 caatgcgcgc gccatcgacg aggatagtca gccgtttgac gacgcgcaac atgtttacgc
     6781 caacatcttc aaccagccca accgctcgcg ccagcaatgg ctgcaccatt ttttttatgc
     6841 cggaaatgcg ttgtgccatc ccagtgtgct gattcgcaag cagtgctacg acgactgtgg
     6901 tctgtataaa ccttggttgc cgcaactagg cgacctggat atgtggatgc ggttgtgcct
     6961 gaaacatgat atccatgtgc tccctaagcc tctggtacgc ttccgtgtac gcgccgatga
     7021 agccaatgcc agcggcaacc gaccggagac acgcatccgc catggcaacg aatcgctgat
     7081 tctgctggac aattatctgc agttgatcga cttcgatcaa atggagcggg tgtttccgtc
     7141 cacgatacaa tatcgccgtg gctccgacag cgaaccgcgc ttcgcgctgg ccatgatggc
     7201 gctcggcaaa cagaacgtcc cgtggcacaa gctatttggc ctgcgactgc tctatgaact
     7261 cttgggcaac cccgatgcgg cggcacgact gcggctgcat taccagttcg attatcgcga
     7321 tatgattcac ctgagcggga aaatcgagac attttgtgac actcaaaatc tttattctga
     7381 gcttgtcgcc attcaaaatt ccaggagctg gaagctgacc aaaccgttac gatggttatc
     7441 tttgcaaatt caattggttc gcaaaaggta gactaatttt gttctggtta tggaaaaaat
     7501 gacatgtttt atttttcgtt ttgctgttcg gctgttgaag cccgcctttc gactaaaggt
     7561 tacgctgttg gcgcgtggtc gggcaatgaa cagaaagggc gttatggcga gtgatttgat
     7621 ggaatcaagg tggtgcatgg acggtttgat ccaagggttg ggcgaatgtg atggccagat
     7681 tgtcgtcctc tataatctgg tcagttggtg gcgtgcacaa tcatttcgta ttgagcacgg
     7741 gaaatgaagc gggttaggcg gactgtcgaa cttgccatct ctgtcattcg gcggggtggt
     7801 ggtctggcgc gcacattcaa gaaagcagtg cgaatatacc gccgtgaagg tctgaccggt
     7861 atcaaacgcg ttctcaggaa ggtaggcaac agccaacttg atcgaaataa ttataacgag
     7921 tggattcgcc gttacgacac gcctaccgaa gaggcatgta ccattatgtc tgcgcgcata
     7981 gatagtttcg tccataaacc gcttgtctca gtgataatgc ctacctataa tcccaaagca
     8041 gagtggttaa tcgaagccat cgaatcggtg cgcaagcaga tctatccgta ttggg
//
LOCUS       DAIANL010000155         5065 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_602660, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000155 DAIANL010000000
VERSION     DAIANL010000155.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602660"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          938 a         1347 c         1816 g          964 t
ORIGIN      
        1 cggatgtcat aggccttgaa aatttcctgg ggcaggacgg acatggaact ctcctgaaag
       61 atacagtggg aatctgctgg cgcgagcata gcctatctgt cgtgtcagcg gcaagcgcaa
      121 gccgcagtgc cgtgagctgg cctgatcagg agggtggaaa tgggatgtcg aggtgctgca
      181 gcatgcgccg actgagccag tcgctgccgg gaaagccgac gtgcccgcct tcggcaggaa
      241 actccagttg cactgctgcc gaaacctggt ctgcgcgtgg cagcgtgtct ccgggcagga
      301 aggggtcatt gcgggcattg atcagtagtg taggcacggc aatgcggtcg agcagcggca
      361 ggctggcagc gcgtttccag tagtcatcgg cgctgcggta tccgtgcagc actgcggtga
      421 agtgctggtc gaaggcgtgc agcgtggtgg cgtgccggac aacgttgccg tcgaacgcgc
      481 cggggtgtcg ttgcagtaac ccggcagcct tgcgtctgag gctgtgcaga aaatggcgtg
      541 tgtacagcca gcggttgatg ccgtgatcga gcgcgtgtcc tgctgcgggt agatcgagcg
      601 gtgcggacac ggcgatggct gcgcgcagca tgggtgttgc cgcgttgcct tgctcgccga
      661 gccagcgcag cagcgcgttc gcacccagcg agtagccgac ggcatacagc gcggcttcat
      721 gtcgcatgtc cagccgcgcc atcacccggc cgatttcagt gctgtcgcca gcgaaatagg
      781 cgcgtgccag gcggttgggt tcgcccgagc agccgcggaa attgatggcc accatacgca
      841 ccttcgcctg tccgagccag tgggccagga tgcgcatgta atagccgtcc gaattgcctt
      901 cgagtccgtg aaatgcgacg accagcggac tgtcggacgg gccgtcgagc cagtcggcgt
      961 caatgaagtc gccgtcgtcc agttcccagc gctcgcggcg ccagccaatg acatgccggg
     1021 gagcgaagaa atagggaaac agcgtttgca ggtgaccgcc tggcagccag taaggcgcga
     1081 tgtaggagga gacagtcatg gggcaagtat aacaagatca cgcgcgaacc ggcatggctg
     1141 cgtgagctgc ggaaatttcg cgggtcactc attgcagcat tcgcaggccg gcggctaaca
     1201 gggtaaaatc ccaacattgc agcggggatg gacatgcgta ttctggggat cgatccgggg
     1261 ttgcggatta ccgggttcgg ggtgatcgac tggcgcgaca atgcgccgca ttatgtggcg
     1321 agcgggtgcg tcaggtcggg cgaaggcgaa ctgccgcagc gcctgggggt gattctcgat
     1381 ggcctgggcg aagccatccg tgccttccag cccactcagg tggtggtgga gaaagtgttc
     1441 gtcaacatga atccgcagtc aacgctgttg cttggtcagg cgcgtggtgc ggcgatttgt
     1501 gcggcagtgt cgcagggatt gcctgtggcg gaatataccg cgttgcaggt caagcaggcg
     1561 gtggtgggca atggccatgc ggcgaaggag caggtgcagg ccatggtggt gcgtctgctc
     1621 agcctgccgg ccacgccttc agcggatgcg gccgatgcgc ttgcctgcgc actgtgccat
     1681 gtgcatggca cacaaggcag tgcggcgtgg ctgtgggcag gacgcacact gcgccacggg
     1741 cgagtggtct gaagcgttca attcaacgga gcatgaaacg atgatgggca ggctagccgg
     1801 tgtgttgctg gaaaagcatc cgccgcagat tctggtggac gtgacagggg tgggttatga
     1861 gctggatgtg ccgatgagca cattttgcca gttgcccgcg acaggccagt cggtggtcct
     1921 gcatacccat ctggtggtgc gagaggatgc gcatttgctg tatgggttcg ccacgctggc
     1981 ggagcgcgac ctgtttcgcc agttgttgcg catcagcggg gtcggggcaa aaatggcgct
     2041 ggcgctgttg tctggcatgg atgccggcga actggcggcg gcggtggtgc agcaggacgt
     2101 cgcgcggctg gtacgcgtgc ccggtgtcgg acgcaaaacg gcagaacgcc tgttgctcga
     2161 actcaagggg aaactgggtg tggcggcagt gacggcttct gcgtcgtcac cggttgccgg
     2221 cccggcagtc gatgatgcgt tgtcggcatt gctggcgctc ggttacaacg agcgtgaggc
     2281 gagtgtggcc gtggggcagg cggatgccgg cgaaagtgtg gagcatcgca tccgcgccgc
     2341 cttgaaatgg ctgtcgcggt catgatggaa ctggaccggc tggtggcgca ggaatcgcgc
     2401 agcaacagcg aagaacaggt tgaacgtgcg ttgcggccgc gccagctggc cgaatatgtg
     2461 ggccaggaaa aagcgcgcgg ccaactgtcg atttttatcg aggcggcgcg caggcgcaac
     2521 gaagcgcttg atcacgtatt gctgtttggt ccgccggggc ttggcaagac tactctggcg
     2581 cacatcgtgg cacgcgagat gggggtcaac ctgcgccaga cctccggccc ggtgctcgag
     2641 cgtgccggcg atcttgcggc actgctcacc aatctcgaac ccaacgacgt gctgttcatc
     2701 gatgaaatcc accgcctcag tccggtggtg gaagaaattc tctacccggc gctcgaagac
     2761 taccagatcg acatcatgat cggtgaaggc ccggcggcgc ggtcggtgaa gcttgacctg
     2821 ccgccgttca cactggttgg ggcgacgaca cgcgcgggca tgcttaccaa tccgctgctg
     2881 gaccgttttg gcatcgtcgc ccgcctggag ttctacagta cgcaggagct gacccgcatc
     2941 gtgctgcgtt cggcagaatt gctcggcatg gatgtggccg aggaaggcgc gcgcgaaatc
     3001 gcatgccgtg cgcgcggcac gccccgtatc gccaatcgcc tgttgcgccg ggtgcgcgat
     3061 tatgccgaag tgcgtgccga tggcagggtg acgcgggaag tggcggatgc agcattgctg
     3121 atgctggatg tcgatcatgc cgggctcgat gtgatggacc gcaaattgtt gggagcggta
     3181 ctggaacgtt ttgatggtgg gcctgtcgga ctggacagct tggctgctgc catcggcgag
     3241 gcgcgggata cgatcgagga tgtgctggag ccatatctga tccagcaggg gtatctgcag
     3301 cgtacgccgc gcggccgggt ggccaccctg aatgcctggc ggcattttgg tctgcaaccg
     3361 gtcgtgcgcg gcgaattgtg ggatggggcg gcagcaggtg acccgcaatg agcgggccgc
     3421 ggcaacagtt cgtctggccg gtgcgcgtgt attacgagga taccgatgcg gctggggtgg
     3481 tgtattacgc caactacctg aaattcatgg aacgcgcgcg cacggaatgg ctgcgtgcgc
     3541 tgggtatgga acagggggaa ttgctgcagt gcgagggtgt ggtgttcgtg gtgaggcagg
     3601 tgtcggtatc ctaccgcatg ccggcccgct atggcgatgc gctccaggtg gtgtcgcaac
     3661 tggtgcagct tcggccgatg cgcatgaggt tttttcagcg tgtcgaacgc gaaggtgtgc
     3721 ttgccgaagg tgaaatagaa gtggtctgtg tcaacgcgca gaccttcaag cccgttatgt
     3781 taccgcgacg ggttcaacaa ttgctggaac aactggaaca ataaacgtgg aaatcggagt
     3841 attgcaagac gtatcgatgg tgtcgatggt atcggacgcc agcctgccgg taaagctggt
     3901 gatggggatg ctgctggtgg tttcgctggc ttcctggtgg cagattttca tcaagctgtt
     3961 cacgcttgcg cgtgcgcgaa accgtacgcg ggaatttgag caggagttct ggaacggcag
     4021 cgagatgggt cggctggcgc agaaagtggc tgccgacggc gaatacaacg gacagatggg
     4081 acgaatcttc gaggcgggat tccgcgagta caacaagctg cgcaatcagc ccggggcgga
     4141 tgccgccgat atcgcagaga gcgcacggcg tgccatgcgc gcatcttatc agcgcgaaat
     4201 ggacgaactg gaaagcaaca tgtcgtggct ggcgacgacg ggatcggtca gtccgtatgt
     4261 cggtctgttc ggcacggtgt ggggcatcat gagcgccttc aggaacctcg ccaatgtggg
     4321 gcaggccacg ctggccaacg tggcacccgg catcgccgag gcgctggtgg caacggccat
     4381 gggcctgttc gcagcgattc cggcgctggt ggcctataac cattatgcgc gcgagatcga
     4441 ccagctggcc aaccgtttcg aaagcttcat cgacgaattc gtcaacatcc tgcagcgcca
     4501 ggcgtacaag cgatgagcgc gcgacgcggc cgccggctgg tggcgcagat caatgtcgtg
     4561 ccttacatcg acgtgatgat ggtgttgctg gtgattttca tggtcaccgc gccgatgatc
     4621 acgccgggac aggttgattt gcccagtgtg gcgaagacgc acaacaccgt cgtagagccg
     4681 atgcaggtca ccattcacgc cgacggcagt ctgagcgtgc gcgaccaggc gagccgttcg
     4741 gcggatgaac cggtgtcact ggacatgctg caggcggtga tcgcgcgcaa gcagaatgaa
     4801 gacccggatc aggcggtggt gatcgccgca gacaagaatg tgcgttatga gaaagtgatg
     4861 caggtcatga gcctgatgca gcgcagccag gtgaagcggg tgggcctgct ggcagtgccg
     4921 gaggccacgc accgatgaag cgggcccgtc aaccggggcc cgccgcatcg tggtccgtgc
     4981 ggacctgggc cggtctgctg gcgctggtgg tgcatgccct gttttttgcg ctgctggtat
     5041 tcagcgtgga ctggaaatcg gagga
//
LOCUS       DAIANL010000156         5353 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_804809, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000156 DAIANL010000000
VERSION     DAIANL010000156.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804809"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1086 a         1444 c         1593 g         1230 t
ORIGIN      
        1 atgttaaata atatggtaaa acgaaatggt gtaatgtcag tgtcgataac ggtaatggcc
       61 cggagactat ggccaacgcg gctggcgacc atgatgtcta aacaaggtgg ccgccgtcaa
      121 tggaaacagg aatactgccg aggaaacagg aatactgtgt aacttgcctt gatgcggcga
      181 gacatcaaat cggagggaaa gacatggcgc gtcccgccag gatcgatgat gcaattgatg
      241 cgatcgaagt tgtgcgccag tcgattctgt cgttgtgcgt ggctgaccac cacaatgacc
      301 gcgctacagt ggatcgctgg ctggccaaca agcacccaga aacctttgtg acctggatca
      361 acgatcctga gaatttttgt gtggtcgacg aaatcaccgg aaaggtccgg ggggttgggc
      421 tcgtgcgcag gaacggcacg gtgctgctgt tctatgtggc tccgggatac cagcgaaaag
      481 gcgttggacg tcgcattcat ggcgcgctcg aagctcatgc ggttggttgg ggtttgcagc
      541 ggctttacct ggaaagtact gcagaggcga ggcggttcta tgaatccatg ggatacatgg
      601 tcgatggccc gggaaaacgg ctgttcggca tgttgatggc gtacccctat agcaaactgc
      661 tgcaacctgg cagccagatc ctgtagtttc ctgtagtttg catcattcat ccgggatggc
      721 agaaaccagg cgcaaccacc tgcttttaat ggcattgccg tcgagaccgg ctcccctatt
      781 ccaccttgat ttccagcgcc tgaaaaatca ggttgtatcc acatcgtcag ttacgaccgg
      841 gacggcgctg cgcctcgtct gcaaccgcaa ccacaaccac gatgtacggc tatgtgatcg
      901 ctgatatccg cgtgtccgga tcacactggt tccgggttca tggcatgagg cacgccctgt
      961 ctaccgcaag catgtctgat ctgcgaacag attatttaaa aacaagcatt aattttcgtt
     1021 acctcatgtt atttgtgctc atgcggctga gcgtcacggc agccatgcct tgagccagct
     1081 gtccagatgg tcttcgagca tccagtggtt gaggacatgt tcgcggatgg cgtcgccagc
     1141 ttgcgccagc gcatcgcggt ccgagatcag ctcgcggatg gtcttgaccc agtcgcggaa
     1201 gcggttgggc actcgccatg ccgggatgtc gttctgatat ggcgtgatgt cggagcacac
     1261 caccggatag ccgagaacgc cgtactcaag cagcctgaga ttgcttttgc cttcgttgaa
     1321 cgggttgtcc tccagggggg cgatggccag atcgagatcc agactggcca gttttgccgg
     1381 atattccggc agtggtacgg ctgggtacac ctcgtggacg tacggggaaa gcgcatccgg
     1441 gcacatgccg aaaaataccc actccacttc attggcgaga ttgcgcacca tgtcggcgag
     1501 catttcgagg tcgccggtgt gaccgatgcc ccctgcccag ccgacgcggg gccgggtacc
     1561 accgcgccgc ttcggtttga ggtgccccca ggtagcgtgt tccagatagt tcggctgtac
     1621 gcgcacttca ccagtgaggt cccggtaggc atgtgccagc ggttccgtgg ttaccagcag
     1681 gcaatcgcag aacgacgcgg ccttgcgcat gcgtttggca atgtccttgt ggatatgggc
     1741 cttgtgaaag ctcttgatcg gcagattggt gagcaggtca tcgagttcga aaatacggaa
     1801 tgtcttgatc agcctgctct gctgttccag cgcggccatc tggtcttctt cgaccggttt
     1861 ctgcatcacg atgctgtccg gcatgatgcg ttcgatttcg gcaggctgga acacgcgcat
     1921 gctctcccac ccctgcaccc ggccggcgtt gcgcagggcg cgcatcggcg caatcatgcg
     1981 gtattcgccg caaccctggc ggtcgtacgg ataggccaga atgcgtgggc gtggtcgcca
     2041 cgccggatcc caggtgagta tggcctggtc ttcgagctgg acgtgggtgt tgtacaacgt
     2101 cagatggcgg ttgtatgcag ggtcaaaagc cagttttggc aaccagcgct gatagaacgc
     2161 cagctgttcg gcctgaaaac gtttgcgttt atccgtatcc ggtttgctct cgacgtcgcc
     2221 tttctgactc gccgagcctt catgcaacag gatggcccac ggcgtccata cgatatgcag
     2281 gccggcttcg cgcactttca tgcacaggtc gatgtcgttg taggatactt tgaacgtcgt
     2341 ttcatcgagg ccgccaagtt gctgatacag cgatttgcgt atcagcagac aggcgccggt
     2401 gacagccgag tagttctggg tcagttgtgc gcgtccgaaa tagccgcggt aatccgcgcc
     2461 gtgaccgatg tatggatgtt ccgccggccc tttcatgccc aaaaccacac cggcatgctg
     2521 aatttttccg tccggaaaca gcagttttgc gccaactgca ccgatttcgg gtcgcaatgc
     2581 atggctgacc agttcatcca gccagtcttc gtgcagtaca cccgtatcgt tgttcagtaa
     2641 cagcagcatc tctcccgcgg cgatttcggc ggcgcggttg ttcatggagg agaaattgaa
     2701 cgcgcccgga tgacgcaaca cgcggatgcg attgccgaaa gccgggcggt tgttttcgag
     2761 cagatcgagg taggtgcagg tctgcgcatc atcgctgtcg ttgtcgacca ccaggatttc
     2821 gtaagccgga tagcgggttt tctcgatgat cgattccagg cagcggcgca gaaacggcaa
     2881 ttggttgcgt gtcggtatga tgatgctgac cagcggcgtg tgctcgcgtt cgtagcgcac
     2941 gcggaaggat ggcggaaacg gtccgcgcag cacggttgcg cggatgccga gtcgtcccag
     3001 gtgacgtgcc agtgcctgct cgcaggcttg cagcaactgt tccatgctga tgtgtgtgcg
     3061 gccggtgctg ccctggcggc gatggtagag cacttcgggc acatggccga tgccgctgtc
     3121 gccgattttt tcccatgcac gcagcagcag atcgtactcc tctgccccgc tcgcctgcac
     3181 atcgaacccg cccagttcgg caaacagcga tttgcggatc agcagcaggc tgcccgcata
     3241 aggcatgctg cgcagataat ccaggttgaa atccggtttg cagtgtggat ggctgtgctc
     3301 gccgtccgca ctgatcgtgt cctcgtcgct gtagatgact tgccattcgg ggtgctcatg
     3361 cgctgcatgc gccagacgga aaatggcgtc ggcggcgatt tcgtctccgg catcgatgac
     3421 ggcgagccat tcgccattgc attgcagcag cgtctggttg agactggatg cgacggatgt
     3481 gcccagcgtg accacctgcc agcgtgcggc aagccactgg ttgtgcaggc tgctgacact
     3541 ttgcgcggct gaatcggctt catgccgttt ggccagcacg atttcgcagg agaacggttg
     3601 ccattgcgca agcgtttcat cggcccattg ctgctcgccg ggcgaccagc gccaggggcc
     3661 gagccagggc tggatggtct gtgacgatgg cgttgttctg gtttgttcgg cgggtaccga
     3721 agatggcccg gcaaacacgg cgtgtttgcc tgtgagcgcc gtcatcgact gatagttgaa
     3781 ctgatacagc ccttcgacgc gggccgaagc ggcaggatcg gccatgatcc ggtagagcaa
     3841 ctgcatgccg aacaatttgt gccagggcgc gctttggtcg gcaagcgcca tcatcgccaa
     3901 tgcaaacgca ggatcgcctt cttcacgtcg acggtactgg atggcggctg gaaaaaccct
     3961 gaccagttca tccatggtat ccaggcgcag gaagttttcc agaatccgga aatattcatt
     4021 ggcgtgccga attcgcgttt ccggacggtt tccgctggta ttggcttcgt tgtcacggac
     4081 acgaaagcgt accagtttgt ccggcatgac atggatttca taacgcaggc acagtcgaat
     4141 ccacatatcg aaatccggca gttgtgccag ccagtctttg taagtaccgc aatctgtata
     4201 gcactgcctg cgaatcagca ggctggggtg acacaaggca ttgccgtaat agaagaaatg
     4261 gtgcaaccat tgttctcgtg tacggtttgg ctgattgaaa atgtttgcgt aaaaatgtcc
     4321 ggtatcccgg agcggcgcgc tgtcctcacc gattgcactc gcattggaaa ataccgcacc
     4381 catttccgga tgcgcatcga gaaaagccac ctgccgggcc agtttgtctg cttcccatac
     4441 gtcgtcggaa tgatgcatgg ccagatattc gccctgcgct ttttccgcga tgatcagatt
     4501 gatcccgtag gccgggccaa ggtttgtttc attacggaaa gcccggatgc gcggatcgtc
     4561 gtagctttgg ataatcgacc atgaactgtc gtgcgaggca tcgtcccaaa tgagaatttc
     4621 gaaatctttc caggtctgcg cgagcagact atccaggctt tcacgaatgt atttctcgtg
     4681 attgtaggaa gtcaggatga tcgaaacttt aggcatttgc ggttctccgg gtctggatct
     4741 tggcggaggg gcacgccccc tcgtttgccg gttttcagtg gctaatcaag cctgttgatg
     4801 gggttttctc gctatcagcg tgtactggat cggcatcaga aaacgttctt catcttccag
     4861 tgccacaagt tccaggattt cgaacgcgtc gaacaattcc ttcaatccgt gctcggtaaa
     4921 ccgccagcaa tcgggaagtg gaccgtgtat gcgaaaattg aacggcgtgc tgacaaaaac
     4981 caatcccccg ggcttgagca tctggtgcat gttccggacg gcatggaacg gttggcgggt
     5041 atgttcgaga acttcagtgc aaacgatgaa atcgtaacgg tccataccca cagtgtctgc
     5101 acaattgcac aggtcggcaa taaaggttgc gccgctggac ggatcgatat caagcgtttc
     5161 gatgcgtact tcatgcttga aaaatggccg ggcgccccga tggtcctgcg gtgccacatc
     5221 aagcagcaat gcgccgggaa aagcgaagcg gttgccgact tcctgcatga atcgctccac
     5281 attggcgcgt agcttcgcaa ggtgttcgat atcgatcctg gcaacggtct ttttcatttc
     5341 cagagctttc atg
//
LOCUS       DAIANL010000157        14371 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2228942, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000157 DAIANL010000000
VERSION     DAIANL010000157.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 14371)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14371)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14371
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2228942"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2974 a         4845 c         4057 g         2495 t
ORIGIN      
        1 cgaaattgaa cgccatgtat tccactcttc agaccgcggg caactctgcc ccaatgcggg
       61 cattgtcacg caatctgccc cggtcgcgca aggtcgcccc atgctgtccg ccgcatcgga
      121 catcgactgc ggggcgtcgc ccggcatcat ccggtagcgt ccatgcatcg ccggagcaga
      181 aacactctgg ccggtcacgg tcatggccgt ctgcagactg cggccggacc gccgcacatg
      241 ggatgccctt gccgcttccg cagcaggtcc ggggtatatc gctcaggcca catgcacctt
      301 gacgcacaac tggtaaccgt gaccgcgcac cgatttgacc ggttgctgtt cagcgccggc
      361 ttcgaccagc tttttgcgca agcgcacgat ctgtacttcc agcgcccctt tgctgaaact
      421 gtcctccgat tttccggaca cttccatcag ttgccagtat tcgagctggt ggccgtaggc
      481 acgggcgaat gccgacagca gcaccgattc ggtggccgac agcacgaccg tagcggccgg
      541 gccacgcagc ttccggctca ccagatcgaa cacgaacggc gcatcgctgc ggttgcgccg
      601 gatacgccga gacagcgcct gcactgcagc caacagctct tccattgacg tcggcttggt
      661 cagatagatg tctgcgccgt tttcataacc gatgagccga tcctcgagct tgccacgcgc
      721 ggtgaccatg atgatggtca catccggctg tgaagcgcgc acgcgacgcg ccaggctgat
      781 gccatcttcg ccgggcagat tgaggtcgac caccaacagg tcgagcggat gggccgccat
      841 tttctcgtcg agttcctcgg cacactccac gccctgcaca cagtggccga tgctgtgcaa
      901 tgcctcgatg gtcacctcgc gcaaggcatg atgatcttcg acaaccagaa tattcaacgg
      961 ggtatccaca attcaaacct cacttcaccg gctgtcggac tgtaacgcac ccagcccccc
     1021 agcaattcca tcacactgcg caccaggtac agccccaacc ctgaaccgga actgccgtgc
     1081 gcccccggac tgcgataata tttttcaaat acacgggcgc cgtccggcat gccggcggtt
     1141 cccggcaggt tgctcacctg cacctgcacg ccgtcgcggc cagcgtgccg ctgtctcgtc
     1201 atgcccaccc gcaccggctg gtccacccga ccgtatttca gggcattgtc gatcaggttg
     1261 ccaagcacga tccgcagcaa cagcgggtca ctgctgagca cgatacgcgg ggcgccctcc
     1321 agcacgatac gttccggcgc ctgcgatgcc tcgcgcactt catccgccag ccgccggata
     1381 cgcaacggct gcaatgcggg ttccaggcgg tgctggtcga gccggttgat ctgcaggaaa
     1441 cgatcaacga tcgcatccat atccatcatc gcgcgattcg cgtgctgcat caccgccgcc
     1501 gatcgcttgt cgaaaccgag cgcaagccgg atcaccgaca tcggcgtctt gatctcgtgg
     1561 ttgagcatgt ccagcaaccg gctctgctcc tcgttgcgca ggtgttcgag ttccagcttt
     1621 tgctgcgcca gttcgagatt ctgtcggttg gcggcgctca gttcgccagt caccgcgccg
     1681 acgaaatgca ccagccggga cacgactcgt gactgtttgc tcttgtacgg atcggtggca
     1741 tcctgctgcc gcagcgggtg cgcatcggcg ggtgacgcat catgatgggc gatagcggcg
     1801 ccctcacgga tacctaccgc aaccagtgcg gaattgagca gacggccatt tttcatccgg
     1861 ccgtaaaact cgccgctggt ataaaaaccg gcagtcggcg caatcgcctc gaatggccgg
     1921 gtttccaggt cagcgtcatc ctgcatgagc agccggcggc atccgcatga gtagaggaaa
     1981 atcgcctgcg gcgcaaactc gcgcatggcg gcatgcacgt ttcgtgcatt gctgacgatc
     2041 acgcccgggt tgccataacc caggcgaaat gtctgccctg cttccacatc ggcgaagaac
     2101 tgcagcgcac cacggtgatc aactgcaacc ggaaccctcg ccagccactc gccatcccgc
     2161 tccatcatga aagggaattc cagcgcattc aggaagaact ggtcgtcagt ctggatgtcc
     2221 agataacgcc ggtagacctc gaaggccggc tcgccatcga ccgaccgcac ccacatcccc
     2281 tcggccgaag tgatggtcat ctcccgactc agcggctgcc agcccaggct accgaatggc
     2341 gccacatgca gctcgcggcc gatcatggcc acggccacca ccccctgact gctcaattgc
     2401 gccccccaca ttacgaacga acccgcctgg gtcacataat ccccggcccc gccgccgatc
     2461 accggcacgt cgagtccgct gtcctgcaga ccgctgagca cacgcgacgc attgacggtg
     2521 atgttggtgc tcagcataag caagccagcc accggttcgc gtatcgccgc aagttcactg
     2581 ccgagcaggc taccaacacg aaattcttct cccggggcgc aatggtcgcc aaacagcagc
     2641 agatcggtcg attcaaagaa cgacagcaac agcaccgccg tctcctgcca ggtccagcca
     2701 tgagccagct ctcccaccgt ggtggcaccg accagcacca ctgaaggcag ggcatcggtg
     2761 atcaccttca tgacgctggc gatccagtcc ggttccatgc gccccgagta gacctgggcg
     2821 agcaccgaac gcgcccgctg cgcttccatg cgcaccgagg gcgaatcgag ataagccacc
     2881 agatcgggca atgtatgcac gacgcaatta tattgtttca tctgttccgc atgacaggta
     2941 tcggacgcgg gttgctcccg gcgcgccgca tcgggggaaa tgacgccccc attgcgcaat
     3001 gttagtattt gatcataccg gaagtcatgc catgtatcca aaccgcatgt gccgacagga
     3061 catgcctgcc tcacgggtgc gatatgcaac tgcggaattt gctagcacag ccagacgacc
     3121 tgcgtgctac aattgagtaa agtacgtaca aaatcagacc gttccccagg ccgccgaccg
     3181 gagcgtttcc gtcgcgacgc ggtggccatg gcggctgggc cgtacaggct cccgtgacgg
     3241 aatcgcaatg gcaaggcgtg gacggactgg ctgcgccagg atggatggat ccgacaatgc
     3301 gacaagactc aacccagaat caggcggcac agcgcgcacg cccggccaca gcgcggcgac
     3361 ggctggccgc tgcggccgat ccggccgacg caacacacgt ccggcaagcg ctccgctccg
     3421 gagaagaaca gggcgcctac aagctgcccg ggttgcacat cctgtccgaa gtagcctcgg
     3481 cactcagctc cgatgccgac atcgaacagc tgctgatgcg tttcctctcc accatgatgc
     3541 gcctgagcgg tgccggcgca ggcgcggtac gcgtactgac cggcgacggc aagcacctgc
     3601 gcctggttgg ttcgatcggt ctgcccgaac aggtcgtcga gtgggaacgc atcacccgcc
     3661 tcgactgcgg cgtatgcggc caggcggtat gcgatcagac cgtgcacaac agccgcaccg
     3721 tgcatctgtg ccgcaagaat tccggcctga gcttcttcgg cgaacgctgc accaatttcg
     3781 tcgccgtgcc gttacggcac aagggcaaaa cgcttggcgt ctacaacctg ttcatgtttg
     3841 ccgaaagtgc gccgattccg caggatgtgc ggctgttttt cacctcgatc agcgaacatc
     3901 ttggcatggc gctggaaaat gcgcgcctca cgcgcgagaa catgcgcatc agcctgatga
     3961 acgaacggca gatgctgtcg cacgagattc acgactcgct ggcacaaacc ctggcctaca
     4021 tgaatatccg cctcaagctg ctcaaaacgg cgatcaacga aggtgacgac agctttgccg
     4081 acaaatgtct gcatgatgtc gacgatgcac tcaaatccgc ctatgccgga ctgcgcgagc
     4141 tgctcaacca gttccatcac cgcatggacc cgcgtggtct gctgccggca ttgcgcgacc
     4201 tgctcgacgt gacttgcaaa aaaaccgatg cacacgtgga tttcatcaac caggcccagg
     4261 atttcgatct cactcccgat caggaagcgc gggtatttca catcgtgcag gaagcactgg
     4321 ccaacatctg caagcactcg caggcgcaca acatccacct ggagatcggc cggatcgacg
     4381 atagctgccg catcatcatc gcggacgacg gtgtaggcat gccaaccacc gcacccactg
     4441 ccccaggcgt gcatttcggc ctcaacatca tgaagggccg agcccggcag ctgggcggac
     4501 agatcgagtt ctccgactgg aatggttccg gtacccggct gacgctgctg ttcccgtttc
     4561 agccccgcca ggaaggaaag gtttcgtgaa cacccgcatc cgggtgctcc tcatggacga
     4621 ccacacgctg ttccgcgaag ggctcgcgga actgctggaa cgttctggca aggtaaacgt
     4681 ggtcggcatg accggtaacc ctgatcatgc gttgcaactg gcgctggata cgcaacccga
     4741 tgtgatggtc atggatctgc acatgcctgg aaccggcggc atcgacatgc tgcagcgatt
     4801 caggcagcag cagatcagtc tccccaccgt gatcctgacg gtgagcgacc accagaacga
     4861 tatggccaac gcattgcgcg ccggcgcacg cggctacctg ctcaaggaca tggaaccggc
     4921 cgacgtggtg caggcgatcc agcgagccgc aggcggcgaa accgtcatcg cgcctgccat
     4981 gaccaccaag ctcgtcaatc tgctcgatgc caagcaggac aagaaaaacc agctgctggg
     5041 ccagctgacc cagcgcgaac agcagattct cggctgcctc gcccgaggtg acagcaacaa
     5101 gatcatcgcc ctgagtctcg gcatcagtca cgacaccgtc aagctgcacg tgcacaatat
     5161 cttcgccaaa ctcaacatca cctcgcgggt ggaggctgcc gtgttcgccg tcgaacaggg
     5221 gttgaacctg aattcgggca acctgcccca ccgcaactag ccgctccagc cacaggctgc
     5281 tgaagacgca atgcaaacgc ccggataccc gttctgcggc acggatgacg tgcaggcagg
     5341 ccacgctcgc aaccggcttg ggcctgccta cacccgcttc ccgtcgccgg ctagttttcg
     5401 gggtgaaacc aggccagttt gctgtgcagg ctcaccacgt ggccgacgat aatcaatgtg
     5461 ggcgccttga atccctgctc gaccgccagc ccgggcagcg tctccagtgt gccgacctga
     5521 acgcgctggg aatcggtcgt cccctgctgc acgatacagg ccggcgtgtc ggccgtcatg
     5581 ccgtgcgcca ccagctcgcg gcacagcact ggcaaaccca gcagccccat gtagatcacc
     5641 acggtttgat tgggtcgtgc cagcgcatcc cagtcgagat cgatgctgtc atccttgaga
     5701 tgccccgtca caaacatgca ggattgcgca tagtcgcggt gcgtgagcgg aatgccggca
     5761 taggctgaca cgcccgaaga cgccgtgatg ccaggcacca cctcgaaacg cacgccgttt
     5821 tcggccagag tttcgatttc ctcgccaccg cggccgaaaa taaacggatc gccgcccttg
     5881 agccgcagca cacgccgtcc cttttttgcc tgctccacca gcagttcgtt gatgttttcc
     5941 tggcgcatgg tgtgattggc gcgttccttg ccgacataaa tccgctcggc atcgcgacga
     6001 cacagttcaa gcaccgcatc cgccaccaga tggtcataga ccaccacatc agcttgctgc
     6061 atcaggcgca gcgcgcggaa cgtcagcaag tcgggattgc ccggtccggc accaaccaga
     6121 tacacctcgc ccacgggggt gccatcggac tgctcggcca gctgctggca caacgcagtt
     6181 tcagcagccg tctcgttacc ggcatgtatc agcccggcga tcggcccctg cagcacgctc
     6241 tcccagaaca cgcgccgcag ttccggccgg gaaaaacgct gcttcacttc gtcgcggaag
     6301 cgcgcggcca gcagggcaag ccgtccgtaa ctggctggaa tcatggtttc cagccgcgca
     6361 cgcagcaacc gcgccaacac cggcgctgtc ccaccactgg acacggccac cacgatcgga
     6421 tcgcggtcaa taatggccgg cagcacgaaa ctgcacagtt ccggccggtc gaccacattg
     6481 accggcagct tgcgctcgcg tgcggcagcc gacaccgcgc ggtcggtcgc atcatcctcg
     6541 ctggccgaga ttgccagcga catgccatcg agcatggccg gcacaaacgg accgggatgc
     6601 cgctccatgc ggctccagtc gtccggcccg gaaaacgccg gcgacaacgc actggcaacc
     6661 acggtcaccc gtgcgcccgc ctgcaaccgc aaccgtgcct tgcgtgcggc aagctcacca
     6721 ccacctgcga ccagacagcg ctggtcgcga agatccataa ataccggaag aaaattcatg
     6781 atactttcgc tgaatgaacg gatatcccgg cataaatgcc cggacggaag ataatttcgc
     6841 tggaacggga aaagccctgg gcactcgggt caacggtggt ttccatctcc tcgcaccagg
     6901 acggatgact ataattcatt tccttgaaaa accacaaccc ctgatgggat tgtaacgcag
     6961 cgcacatcat gtgcacctgc tcacccgctt cgggtgcgac gatgccgcca cccagcagtt
     7021 gcccggtttc gccatcatgc accatttcaa tgaacccttc gaggtcgtgg caggcacgcg
     7081 acttgcccga tccacccaga ttcgaacgcg ccacttcctc ggagaaaccc gcctgctcgg
     7141 cctgttcttc cgtcagcccg acaaaagcca tttccagtgc ggaatggatc acaaatggca
     7201 ccgtgaagta gttcgcctgc acccggttgt ctgtcaacgc attgattgcc gccaccttgc
     7261 catcatgcat cgccaccgcg gccgtcatcg gcgccgccat cgggctgcgc ttgacatcac
     7321 ccacggcata gatacccgcc acgctggttt cgagacaggc atcggtgaga atgaatccct
     7381 catcatcggt atgtacgccc agctgttcca gccccagccc ccgggtatgc ggagtgcgcc
     7441 cgaccgccac cagcacgtga tcgcactccg cagtactggc atcggcaaaa accacctcca
     7501 gccgcgaccc gtgcccccgc acctgttgca cggtgaggtt tttgtcgacc tgcaccccga
     7561 tgcgcacaag cttgcgttcc agcacctgcg ctgcacggtc ggacacacca tgattgcccg
     7621 gcagcaagcg ttcaccctgc tcgatcaggc gcacctgcgc gccgaactgg tgcagcatgt
     7681 aaccgagttc gacgcccact gctccaccac cgatgcacag tatcgaaccc tgcaccgcct
     7741 gccgcgcgga cagcaactga cgcgaattga atacccgctg accatccgtc gggcagccgg
     7801 gcaattcgcg tggtaccgag cccgtggcga tgacggtacg caacgcgcca atttcgaacg
     7861 ggcccgcatc gtccatcggt acgatacgca accggtctgg cgccacgaat gccgcctcgc
     7921 ccagatacac cttgacgccc atgcgtgtca gccacggcat gaacgtggcg cgcatggcgc
     7981 gcaatacttc gtccttgcgc tgcaacgcag caaggaaatc gccctgaacg cgtccgacca
     8041 gcccccggcc gtcgagctcg ttgatccgct ccatcagtgt cgccatgtgc agcagggtct
     8101 ttttgggaat gcaaccgtga ttcaggcagt tacctccgaa ctgatcgcgc tcgatcagtg
     8161 ccacgcgcgc accaaggtgc accgcgttga gcgcggcctt gtagcctccc ggcccgctac
     8221 cgatcaccac cagatcaaac tgctgcatgg catgcgttcg cagtggatca ggcgcagccg
     8281 ccaccgccct ggtctgacga acagccgcct ttgccgcaac cgccacatga cgacgagggc
     8341 gcatgaaaca cgaaaccgcc cttgcccggc tcaagttcga caaagtcgag cgtaacgccc
     8401 tcaaacagtt cctgctggct tgcatggata agcagttcaa cgccttccac actgaccaca
     8461 aggtcctgtg atccgcgatc gtcaaacccc atggcaaatt ccagcgatcc gtcatcagcg
     8521 cgacgcgccg cgacgcgcag cgccatgcct accatatcgc cgcgatcagc ggcatcctga
     8581 atctgacgga tggcattcgg agtaacgata accatgcaac ctccagttgt ttgaccaata
     8641 aaacgtccgc cgcagcccgc atcaggcagc cagtgccggt gactgcacac cgccaccccc
     8701 agccatgtct cccccggcgc gacgcatcac ccgctgcaaa aaggcatcga cccagctaaa
     8761 ctgccgcgat gaacgcagat gcgcatacga cgccagcagg ttgccgtgca cgatgccatc
     8821 gttgcgacca tcgatgccat gaccgcgctc gacccgccag gcatatacgg tatcggccgg
     8881 caggttctcc agactggagt aatggaactc gtgtccgtga atgtcgcctg cgccaacggg
     8941 ccacgggctg tccgcggtgg ccgacaggcg cacatagccg cgtcccaccg gacgttcgtg
     9001 catgaccaca tcgccatcga tggcgcccac catctggcgg gtctcgcccc gccagctcag
     9061 gctccgcgcc agatacatca gtccgccgca ttcggcgtac accggcaggc catcgtcgat
     9121 ggcatggcgg atcgctgcgc gcaggggcgc attggcttcc agttcacggg caaacagttc
     9181 gggaaagccg ccaccgatga ccagcccgtc cagctcgggc aaggcgggat cggcaagcgc
     9241 atcgatctct accagggtcg cgcctgcggc cgtcagccgg tcaagatcgt cagcataata
     9301 aaacccgaag gcacggtcgc gcacgatgcc aatgcgccag cgtggcaccg ctgccgcctg
     9361 ataacgtgct gcatccggca cgcagtctgc cgcagccgtg cccgcgcagg ccgggacacc
     9421 gccggtcacc tgcagcaggc gctcaagatc cacctgttcc gccacgctgc gggcaatgta
     9481 atcgatctgc cggaccgccg cgtcggcctc gttgcccggg atcagcccga gatggcgttc
     9541 atcgatctgc aaggcaggat catgctgcac cgcgcccaga acaggtacat cggtgtaatg
     9601 ctcgatgatg gcgcgcagct tgccttcgtg gcgcgcaccg cccacctgat tgaggatcac
     9661 accctggatg cggatgtcgc ggtcgaatgc ctggtaaccg agaatcaacg gcgccacgcc
     9721 acgagtcacg ccacgcacgt caatgaccag aatcaccggc agcccgagca attgcgccat
     9781 ggcggcattg ctgttgctgc cttcgatatc gagcccgtcg tacagcccct tgttgccttc
     9841 gaccagcgcg acctgtgccg gcgcggacag ccgctgaaac agttcgctgt tttcctcgcg
     9901 cgtgagctgc tgaaaatcaa ggttgtaaca cggctggccg gccgccatcg acagccagat
     9961 cgggtcgata tagtccggac ccttcttgaa cggctgcacg acgtgaccga gccgatgcag
    10021 cgcggcacac aggccgatac tcagcgtcgt cttgcccgac gacttgtgcg ccgccgaaac
    10081 caggaactgt gacacgttgc gctcaggcgg cagccagcca gcgggccgga cggcgctgcc
    10141 acaattcggc agccagggtc tgcgccgctt cgatggaatc agccatgccc gcggcacgca
    10201 gcgcaatggc cgccgtgccg gtcaccgtcg ccacgccata ctcgtcatcg atctcgccat
    10261 gccacagcgc cggcaggcgc cgcatgtcca tggtttcgtc cttgacgtgg cgcgtgccga
    10321 acagggccgg ccagtcttcc tcggaaagca caccgtcgtg aacgtatttg accagacagt
    10381 ttgcatcagg attgcgctcg atctcgccac cttcgccctt gagcacggcc agatggggct
    10441 gacccgccaa ttgcgcagcc aactggtgca ggtcgcggta gccgggatga aagatgccct
    10501 gcatcatcac cggagcacgc atcgggttca gcatgcgcac caccgtgttc accggtgaac
    10561 gcaacccgag aatcggccgc atgcccatga gacgatgcag cacggggttg agcgattcga
    10621 gcgtcatgaa cgagaaatgc tgtgcacgca gctgctcggc ggcctcccga agggtagcgg
    10681 ccggagtgat ccccagcagc gcaagcgcct cgggtgtata aatgcggcca tcatcacgtc
    10741 cgccatcacc atgcatgaag acgctcaggc catgacttgc cagcagcagc gccgacaaca
    10801 ggaaccatgg cagctggcgg cgcttgcccg cgtacgacga ccagtccacc cgcactgccg
    10861 gcgccttgtc cggcagcgga agggcttcac gcgcggccat cacgaacccg gcaagctctt
    10921 cgggcgattc ttccttgaca cgcatcaaca tgagaaacgc gcccagttgc tccggctcga
    10981 cctggcctgc cagaatcatg cgcatcgcct gctgcgcctc ttcgagcgtc agcgagcggg
    11041 aaccgttgcg tcccttgccc agcgtgcgga caaatactgc aaacgggtgt tcttcattca
    11101 tgcggaaacc tcctcggacg tcagtcggtg cagccgggac ttcgtcaggc tgccttatgg
    11161 tgaggatcca cgaccgcgtc ctccaggctc tcgggcagga aacgcagaat cttgattgcg
    11221 aacacaacga tggcaagcgc cagcgcgacg ccgccgatgc ccagcagcgt ttccaccagc
    11281 gaaggcgcgt acggatgcac cacgccatca tagaaactgc tgctcacatc catgccgggg
    11341 aacaacggca gcggataggc ctgaccacca atgatcgtaa catacatttg cgccagtccg
    11401 ccgaacacga ccagcaggct ggatacggca accgccatgc gtgactgacg tgtggccggc
    11461 gcaagcaaca gcgccagtgg cacgatgccg ccaagcacga cctggccgac ccagaacagc
    11521 gtggtgtaga cgccgccatc gagcaggatg aaacgctcca gaccgtgatg cttggtgaaa
    11581 tacagattgg tcagatgata gaccagcgta aaatacagca ccgctgcgat gaacactgcc
    11641 agcagattct tcagccggcg caccaccgca tcacccagcg gacgcttgct ccagcagtag
    11701 gccgacatga gcaccagcag atagattgcc agaccgtaac tgaacgacat gatgatgaac
    11761 atcggcgcca gcagggccga atcataggct tgccgtgcca cgaggaatcc aaagatcgaa
    11821 ccggtacctg tggtcagcgc cagccgccag atgaacgctg cgacaccaac cggcttgtac
    11881 agcgccgaga ccgtacggtc catgatggtc cagacataag ccgcagcaaa ggtgaagaat
    11941 ccgctgtaca ggaacacgtt ccacgcgaac accgacttga agttgtaatg cgtcattgcc
    12001 acgatcaccc gatcggaacg accgaggtcg agcatcagta ccatcagtcc cccggccagc
    12061 agcgcaatgg ccagcagcgc cgacaacggc gccagcggct tgtagaaatg cttgccgaac
    12121 acggtagcga tggaagcgac gttgagcgct cccgaggcgg ccacgatcag gaacaccgcg
    12181 aacacgtgcg gcatgcccca gacgacctgg ttggtcatgc cggtaatgat gtgtccgtaa
    12241 tgctccatct gccacgccga cagcaggccg gccaccacca gcacacccag tccgcccagc
    12301 aggccgaaat aggcaagact gttaccttcc agttcacgat aaacgatgcg catgacttat
    12361 ataccctgat aacgtacgcc ggtatgcaaa tccagatccg ccagaatctc cgtactggcg
    12421 tagctggcca gttgccgtga aatctcgctc tccggatcct tgagatcgcc gaagaagatc
    12481 gccttgtggc cttcggcgtt gcaggcttcc tggcaggcgg tggtgcgcag accggcatcg
    12541 atgcgctgca cgcacatcga acaggcttcc acgcagccct gcccgcgcgg cacttcagcc
    12601 aactggtccg tgaccggttg gtgaatgaac gaacgcgcct tgtagggaca ggccatcatg
    12661 cagtaccggc atccgataca gatgtgccgg tcgaccagca cgatgccatc cgcacgtttg
    12721 aatgatgcgc cggtcggaca gacatcgacg cacggtggct cctcgcagtg ctggcacatc
    12781 accggcaggg agaagccaaa gccggtacgg ggattcttca ccgtcacctt gcggatccac
    12841 tgcggatcag tcggcgacgt ctggccggac atgccgtttt cctggttgca gccacgcacg
    12901 caggcatcgc agccggtcgc gcaacgcgtg gtatcgatca gcatgcccca gcgaatcttg
    12961 cctgaggcag gcagatcgtc gttatcggcg cgagcctggg cttgccacag catgactccg
    13021 gcaggcagag cgcccaccgc ggccaccccc accatgcccg tcacgaaacg acgacggtat
    13081 gcgtcaaaat cactcatcgg accactcctg ccaatgccag atccctggcg tcgaaccgcg
    13141 ttgccgttcc ggcctgaccg gaatgcgcct gcgccgcctc agcgtgatac atgcgtgcgc
    13201 ccgcaccctg cgaaaccgcc ggatggaatg ccgaagcaga ctgggaagac ggcttgttcg
    13261 aatgacactc gaaacaggtg atcttcaccg cggcatacgc atggcaactc tggcagaaat
    13321 cgcccgggcc ggtcacgctg ttgtccttgg cactggcatg acaattgacg caattcacga
    13381 tactggtctt gtcagccagc tggttgtcgc cctggtgcac ggctcccacg cgatgatgca
    13441 tcagcagctt catgtggttc ttgcgcatcc aggcggtgtc cgccacgcac ttgtcgcctc
    13501 tgggctgcac gatgtgcggc ctggcaacgc cagaggacgc attatcggca tcctcgtcgg
    13561 caaaggcggt tgccagcacg ccggccagca gcgcagcaac cagcacaacc cccttgaccc
    13621 atcctccgcc cagtctgttc atggcgcgtt actcgcccat gcccatcttg atgtagcccg
    13681 tcgggcacac atcggcgcag atatgacaac cgatgcactt ggcatagtcg gtatccacat
    13741 agcggccggt agtcgccttg tcacgcttga ccttgaacac ggcggcctgc ggacagtaca
    13801 ccacgcaatt atcgcattcg aagcacaatc cgcagctcat gcaacgcttg gcttcgctaa
    13861 ccacctgctg ctccgacagc gcaaacaacc gctcctcgaa gtgccccagc gcctgttcgg
    13921 cagtcagggt gatggtctcg cgcaggttgc gctggacaaa ctggaaatgc ccaagaaaca
    13981 gctccttggc cggaatgatg tacttgcccg aacgatcctc gaagttgtgc accgcgtaag
    14041 cactgctgtc cgaaccccgt gcctgctggt gatgatagct ctcgggcgga actcctttct
    14101 cagccagctt gcggatcacg tcgaaccggt gcacgtccac cttgggccgc ttgtcgagat
    14161 cttcattgcc ggtgagatag gcatggatgc cgtcagcggc gatcgacgca tgaccgatgg
    14221 ccgtggtcag caaatgcggc ttgatgacgt cgccgccaac gaagatatgc ttcttgccgg
    14281 ggaagcggta gttgctgtcc gccttgatca ggccattgtt gttgtcgaac tcttccagcc
    14341 cggtgaaatc gacagcctgg ccgatggccg a
//
LOCUS       DAIANL010000158         4731 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3053218, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000158 DAIANL010000000
VERSION     DAIANL010000158.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3053218"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          919 a         1510 c         1329 g          973 t
ORIGIN      
        1 cgtccagcgt cttgcagtca atacacaagg ttgccgtggg acgcgcttca agtcgcttca
       61 ggccaatttc cacaccgcag gcttcacaat agccatattc ttcgcggtcg atcttggcca
      121 gtgtttcgtc aatctttttg atcagcttgc gttcgcgatc ccggttgcgc agttcgagcg
      181 ccatgtcgga ttcctggctt gcacgatcat ttggatcggc gaacagggta gcatcttcct
      241 gcatggcatg aatggtgcca tcgatatcct gcgacaactc ggctttccac gcttcgagaa
      301 ttttgcgaaa atggtccagt tgcgccggat tcatatattc ttcacccgac ttgggctcat
      361 acggggtgaa cttggtgaac tctgctgccg tcatgtcgtg tatcgcttcc gagtggtaac
      421 cgaaaaccga cttaatatca gaaatatgga tgaagatcaa tacaaatgac acagcctgca
      481 cttgccgccc tgctgttcga tgttgacggc accctcgccg acaccgaacg cgatgggcac
      541 cgcccggcat tcaatgccgc cttccgcgaa cacggtctcg actgggactg ggatatcgaa
      601 ctgtatggca agctgctcga ggtcaccggg ggtaaagagc gtatccgtca ttatgtgcag
      661 cactatctgc ccgactatgt gccacccgcc gatttcgacg acctggtgat atcgttgcac
      721 cgcagcaaga cgcgccatta caccgaactg ctcgaacggg ggggcattcc catgcggccg
      781 ggcgtcgtcc gcctgattgc ggaagcccgc gaagcaggca tgcgtctggc catcgccacc
      841 accaccacac cggaaaacgt caccgcactg ctgcgttaca gcctggcagc ggatgcagcc
      901 gactggttcg aaaccattgc cgcgggtgac atcgttccgg ccaagaaacc ggcaccggac
      961 atctacctgt gggccttgca gcgcatgaac ctgacgcctg cggcatgcat ggcgttcgag
     1021 gactccatca acggactgcg cgccagtctc ggcgccggca ttcccacgct gatcaccgtg
     1081 aacgattaca ccgcggacca ggaattcact ggcgcgatgg cggtactcga tcagctcggc
     1141 gaagccggtt cgcctccccg ggtactgaaa gggttgctgc cgtcagccgc gccggtcgac
     1201 ctgacaacgc tgcgaaccct gcacgcaacc gcccaacgtc cgcaacagac ggctccgaac
     1261 tgaagaagcg ccgaccagac gttccttcat gagccgcgtt gctgccggaa gcggaacggt
     1321 gcgccacggc cggcatgaag catggctggt gaactgttct gcattagaac aggccgttga
     1381 aaaagtactg cagcacccgt ctgcggcgtt gctgcgatac tcactccaac gcctgtcgat
     1441 ttgatatgtc tcgtcgttgc gtttctcgcg ccttgcatcc tggcgtccgc gctacctttt
     1501 tcaacggcct gttaaatgtc ggcggttacg tccggtttgc aggatcgttc gcgatcctgt
     1561 ccgccacgga tgtgtcaatg acggaaaccc gtgcggaatc cacgttgaac gccatggtga
     1621 cgcgcgtgtc agtgggtgat gcgcagcccc ggcgcacacc gtcccgcatc gttttttgaa
     1681 caggaatcga acccgcaacc cctggtttcg gagaaaaacg gccattccga ccatgacccg
     1741 aacgggtcct ggtgcgccgg agatgacccg cgcttcccta gccgtgctgt ccggcgatcg
     1801 cacccgactt gaacagcgtg tcgcgcagtt catggtcgaa tgccggatca tggcgccgga
     1861 tccattcgag caccattgct gcatgctcct tttcctcatc ccggttgtgg gcaagaatgg
     1921 cgcgcagttc ctcatcttcg caggcgtcga cccgctggtt gtaccagtcg acggcttcga
     1981 gttcttccat cagggaaaca atggcccgat gcatgtcttt ggtggccacg cttaacttct
     2041 caatgggttc atgataacct tcgttcgcca taattaatta tccctcttga tcagacattg
     2101 aactccccgg ccacccgctc cacccactcc gactcatccg gtcaagtcat gcatcaggca
     2161 agacttgtcg gcctcgaaca ggcttctccc agcgtccgac tgttccgcat ccgctgcgat
     2221 gtcgattcat tctaccgtcg ggccggccag tggatcaacc ttgcccgcga actcgatggc
     2281 acgcttcata cagccagttt ttccgtggcc tcgtcgccga cagacgccgg cacaatcgaa
     2341 cttgcgatca aacacagcac ccgccatccg ctgacgcgct ggctgcacga acaggctcag
     2401 cccggggaca gcgtgctgat cagcgatggc cagggtgatt tcatctggga agcgtcacag
     2461 ccgcaacgcg tggtgctcat cggcgccggc accggcgtgg tgccgctcat gagtattttt
     2521 cgtgccatcg ccgaagccgc tacggttcag gccacgttgc tgtacagcat ttccagcccg
     2581 gaagagtatc tgtttcgcga cgaaatcgtc cggttgtcct cccgtcccgg cttgcgtcac
     2641 attgtgacgc tgagccagcc caacccgggc tggcagggtg aaaccggacg cattggcgaa
     2701 tacctgctgc ggcgcgccgg actggaccag caaacgttgt attatttgtg cggcccgcaa
     2761 gccatggtag acggcaccag cgatctgctc gccacgctgg gcgtgccgac cggacacgtc
     2821 atctacgaaa agtggtggtg agccggtcgc ttcagtcagc gtcgtcctgc tgacacactt
     2881 ctaccgtccc atgctccgct tgctgcaagc cgtctgacgc tggcgcggta tcggcgggca
     2941 tctgcgcagt ttcggcatgt tgcgaatcgg ctggcacctg cacagtctcg gcacgttgcg
     3001 tgtcggcagt cggttcggtc agatcaggcg tccgccgcac cgggggcagg aataacgcgg
     3061 ccacttcgac tggccgccgc accgatcgcg ccatgggcat gagcggcagc ggttcgtcat
     3121 cattactgcg acgacgcgat cgcgcagcgg caggttctgc ggtcgctgcc cgcaccggag
     3181 ccggggcggc cgccatcggc aacacctcgg tcggctcaac ttcgatctgc acgcccagca
     3241 actgctcgat gtcggcaagc gcacgcagct cttcctcggc cacgagcgac aatgcttcgc
     3301 cttgcgctcc tgcccgtccg gtacggccga tgcgatgtac ataatcttcc gcgacacgag
     3361 gcaggtcgta attgatgact acgggcaact gctcaatgtc gataccccgc gcggccacat
     3421 cagtcgccac cagcacgcga atctcgccgc gcttgaactg atcgagcgcc tccacgcgcg
     3481 cctgctgact cttgtcgccg tggatggcgt tggctggaat gccatggcgg cccagatgct
     3541 gtgacaggcg attggcgcca agcttggtcg cacagaacac cagcacctga cgcaggtcgc
     3601 gcgaacggat caggtgcgtc agcagcgccg gcttgtgctc gcgttccacc cgaagcacgc
     3661 gctgtactac cgtctcggca gcggtgttat gccgcgccac ttcgatcaca cgcggcatgc
     3721 gcagaatgct gtcggccagt ttgcggattt cgcccgcgaa tgtggccgaa aacatcatgt
     3781 tctgccgttg ctccggcagc agttcgatga tgcggcgcag gtcgggcatg aaccccatgt
     3841 cgagcatgcg gtcggcctcg tcgagaatga agatccctac ctgcgacagg tcgagcgtcc
     3901 ggttctgcac atggtcgagc aggcggcctg gcgtggcgac aagcacatcc actccttcgc
     3961 gcaacttgga gatctgtgga ttcatgttga cgccgccata gaccacggtc acgcgcagag
     4021 gcaggtactt gccgtatttg cgcacgctct cctcgacctg aatcgccagt tcgcgcgtcg
     4081 ggaccagaat cagcgcacgc accggatgcc gggccggcga cagactgttg gtcgcaaacg
     4141 gcgccagccg gctcagcatg ggcagggtaa acccggccgt cttgccggtg ccggtttgcg
     4201 cacccgccat gatgtcgcac ccggtcaggg caaccggtat cacctgcgcc tgtaccggcg
     4261 tcggttcgcg ataaccgcat tccgacagtg cgcgcaatac ctcgggcacc agaccgagat
     4321 cagcaaaacc catgagcact cccatcacgt taacggcact attctacagc atacctgcac
     4381 aatcttgaca aaataaccgg caattgcaac ctgcgcttcc acaccgccat attgtcgagg
     4441 ttgattcgcc agcaagattt aatatataat taaaagctaa tatagtaact tgttgacgca
     4501 ccggcaaaaa ttaaccattg ctggtaccat gaattcttgc agtacgctgg caatcatcca
     4561 tttgaggttt tcagtccgat gaacagatac cgaaccgata cccggccacg gctgggtctg
     4621 cttgcctttc tgatcggttg cgcctgcagc ttgccggcgg gcgccgagac tctcgatttc
     4681 gcccagtgcg tccgcactgc actacaacag aaccctgacc tcatgggtag c
//
LOCUS       DAIANL010000159         8060 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3263133, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000159 DAIANL010000000
VERSION     DAIANL010000159.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8060)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8060
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3263133"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1593 a         2576 c         2368 g         1523 t
ORIGIN      
        1 cagcgtgccg atcagccggt aatagctctt tgcgaagacc atgccggaat gctgcgattg
       61 catcacaatg gcaaccgtca tcatggctgt tctgggctgc tgcagctcga agcgcaagga
      121 aaaccacatt gccagcagac aggccagcag taccttgaac acgtagatgt acgccggcac
      181 atccagttcc agccagtcgc gccagcccat gcgggcgaat gccagcacct gcaccctgtt
      241 catctggcag gtatttccgc cgccccacca cccagagcga gcatcagatc ggcataggct
      301 tcggatgctt ccgcatgcac ttgcgcgagg gcctgttcct gttgcagcac actgtcataa
      361 gtgtccagcg cgcgcagata atcactcaac cctgcccgct gggctgcaag ggcgaattca
      421 tgcgctttcc tggcctttgc gagggcgaga tcgatctgcc ttgcctgcgc agaatccgat
      481 tgcagttctg ccagcccgtc tgaaacctgt tccagcgcat ggagcagcgt ttcgttgtag
      541 gattccacgg cgacatcgta gctggcagta ctggcagaca gtcctgcgcg aagacgtccg
      601 ccatcgaaga tcggcaggga aaatgcaggg ccggcgcctg cgatcagcgc ggcattgctg
      661 gcgagctgct cgaaaccaag cgcctggaaa ccggcaaagg cgacaagatt gatgttgggg
      721 tagaacgccg ctcgggccgc ctggatgtcg ctttgcgccc cctcgatacg ccagcggcag
      781 gcgatgatgt ccggtcgcct cccgacgaga ttggctggca ggctgtccgg cagacccacc
      841 ggcgcatcca gcgacatttt cggtcgaaca agcgattctc ccgcacctgg ccccatgcct
      901 gacaatgccg ccagctgatg cttgagcacg agcatgcgcg catcgatcgc atccaccctg
      961 gcctgcgcca caggcaaccg tgcctctgcc tcggtgactg ccatttgcgt gcccagcccg
     1021 gctgccagtc gtcgtctttc gatggcaatg cgcagatcaa gctgttcgag gcgccgctgg
     1081 gcattttcgc gcagagaaaa cgccaacgcg agtccgatat aactgcgcac caccgcattt
     1141 tccagttcca gccgaacctg ctgtgcttct gcagcagaag catggacggt atcgaccgaa
     1201 gatcgccatt ggctttttaa gcgcccccac aggtccagat catatgaaag cgagaacgtg
     1261 gcactgttgt tccagtcagt gtgccctccc cagggaggcg ggatgaattg cagcgcggta
     1321 aagcgttcgc gcgtagccga tgcgtccgca ttcacctgcg gtgcagtagc gctggcgcgg
     1381 gcatcagcga acgcctgcgc cagcgcgacg cgcgcctttg cggcccggac ggtgggactg
     1441 cctgcaatgg cctgcgtgac caggccgtcc agctgcggat catgatagat tgtccaccag
     1501 gcggtttcag gccactcgat gcgagcgctg cccttgatca ttctgcctgc atcgagcgta
     1561 ttcgcatcct tgagactcga ctgcggcacg atggctggca ccccggcaca cccggccagt
     1621 agcaggccga ttaccggaaa gattgcctgg cgcagcacgt tcatgatttt agtacggtcc
     1681 ctaactaaat ggccaaaaaa aactgctatt gcatgacttc gacattggcc gccagcgcat
     1741 tgacgcccgc gaccagtgaa tcccgttcag ctgtgcccag cagcgacatc aacgcatgca
     1801 ctttccggta atagtcgggc atggcctcat ccagcttgat ctgtccgcta tcagtgagcc
     1861 tgatgaggaa tttgcgtctg tcgaccgggt ctgtcagacg ctgtaccaga ccttcgcgtt
     1921 ccaggccatc gatgaagccc gtcatggtgg ccttgctcac tccggcctta tcggccagat
     1981 cggacggaga cgaagtcagg tcatcctgac gcatcagcag caccagaacc caccaccggc
     2041 cctgaagcaa cccatgccgc gcaagcagcc tgtcgagtgc cagcgaaatg tccgagcatg
     2101 cacggagcag gttgacgaaa tctgaaacag cccggatatc ggcgtcggga taacgctcga
     2161 caaatttctc cagacttttc gaggttggca gttctttgag caatagcatg cggaaaatta
     2221 tagtacggct ccatataata tggcaacaga ctataactca agtaatcaaa tttcttgcag
     2281 ggcaacatct tccatcatgg atcggcgcgc aacacgtgga tcggtgcgca acgacccggt
     2341 ctactggctc ccgctcccgt caccccgctg tttcaacgga atgaacctga cgcctccagc
     2401 cggctggcgg gtccggactc tcctgtacgg caagcctgac acggcatgcg cggcaatgcc
     2461 cgggcgggtg cgctgcctgt cgccacacac cggtaccggc acattacgca tgcccgaccg
     2521 cgaaagtttt ctgctgttcc tgtgaccggc ggtgtatttt atgtataata gaaaacgcaa
     2581 tcaacaattg ctttcaattg aaaaaatatt ccaatgaaag gcgggggcat gaacactctg
     2641 atattggcaa ccttgatgat cgctacgctg tccatggcag gctgtgcctc ggtacccatg
     2701 gcctccgaag ccgatgatgc agctggcaag acatttgcag cacccgcggc agacacggcg
     2761 ggggtgtaca ttttccggga cagttttatg ggcaaggcgg tcaagcgatc ggtatcactg
     2821 gatggcgttg tcctgggaaa tacggcaaac aaggtgtatt tctacaaatc cgtgtcaccc
     2881 ggatcgcata ccgtatccac agaatccgag tttggcaaca agtcggtgag ttttcaggcc
     2941 gctggaggac acaattattt tgtacgtcag gtcatcgaga tgggtgtgat ttccgctgcc
     3001 gcgaatgtcg agatggttga tgacgcggaa ggtgaaaaag aagtactcca atgcgatcgc
     3061 gcgcaacaac aatgagttgg ggacgcggtg atcaaatccg cgtccttggc aatatccccg
     3121 gctattccac ccactgcacc acttcttcag cactgcgacg gattttggca gcgggtgact
     3181 ggacacggta accaaacccg gccatgaccg cgagtcccca gtgttgtcgc accatgatac
     3241 cggcatcggc aagcacggtt tcggcggcct cctgctcgaa tccctcgacc gggcaggaat
     3301 cgacaccgag catggccgcc acggtcatca tgttggccaa cgcgatgtac gactgacggc
     3361 atgcccagtc gaacagcgcg cgttcgctgt cgagcagatg gaaatcgtgc tcgaggaact
     3421 tctggtaggt tttgctgcgc agtgcaatgc gctcgggcgg aaattgcagc acgtcacgca
     3481 acatatgctg cacgtattcg ccatcggtag tgagtccgtc gcggcgggca agcagggcga
     3541 catagtggct ggccgacggc aactgttttt gcgcgcccca ggtcacgggc agcaagcgtt
     3601 cacgcagcgc agcattctga atgaccacaa tgcgccaggg ctcgtagccg aacgaactgg
     3661 gcgagagccg ggccgcctcc agaatgaggc cgaaatcgct gtcgctgatg cggcgcgccg
     3721 gatcgaacgc cttgcaggca tggcgaaaat gaaatgcgtg cagaatgtcc tcttgcgaaa
     3781 tcatgcgata ctcctgaagt acgcggccat aaatactggc ggcaagacct gacggatagg
     3841 ctggcgtcgc gtggttgaca accgcttcac gcgccgtcca tcatggtatc aattattcgc
     3901 cgggttcgcc catgaaccgt ctgctgtcgc tgcatgttgc cattctgctc agtgcgctgc
     3961 tggcacttgg gtgcggcttt gtgctttcga cggccggtca cgcacagctt gccgcgtatg
     4021 catgggtcgc aggctcggtg ccggccctgg tctggctgat cctggatctg gcgcgcacgc
     4081 tgatgcgcgg cagtgccggc atcgacatcc tggcactgat ctccatcggc ggagccatcg
     4141 cgctccatga atcgctgact ggcaacgtca tcgccgtcat gctggccagc ggccagttgc
     4201 tggagagcta cgcccagttt cgagccggtc gcgaaatgac gatgctgctg gcgcgggcac
     4261 cacgccatgc catgcgcctg caacagggcg aactggtgcg catcgatctg gaccaggtgg
     4321 cgccgggcga ccgtctgctg gtacgcagcg gagacacggt tccggtcgat ggtctgctga
     4381 ccagtgtcga cgccacgctc gatgaatctg cgctgagcgg tgaaagcgcc gttgttgagc
     4441 gccatgtcgg cgaccggctg cgcagcggtg cgatcaacgc tgcatccgcc tttgaaatga
     4501 ccgccactgc cagcgcagcc gacagcacgt ttgccggtat cgtccgcctg gtggaacagg
     4561 cgcgcagctc acgcgcgccc gcttcgcgac tggccgaccg ctatgccctc gggttcgtgc
     4621 cgctggcgct cggactggcc ggactcgcct ggctggtcag cggcaacccg ctgcgcgcac
     4681 ttgcggtggt cgtggtggct acaccatgcc cgctgattct cgcggtcccg gttgccatcg
     4741 tatcgggcat gtcgcgagca gcgcaacgcg gtatcctggt caagggcgca gcggcgctgg
     4801 aaatgctggc gcgcgccagc atcctgtttt tcgacaaaac cggtaccttg accagtggca
     4861 acgcccgtct ggtcaagatt gtggccgcac ccggcatcga ccgcaacgag gtattgcgac
     4921 tggcagcctc ggtcgaccag gtttcggcgc atgtgaccgc cgccgcgatc gtatccgtcg
     4981 cgcacgcgcg tcgcctggcg ctggaactgc ccaccgacgg catcgaaaca cctggcgcgg
     5041 gcgtcgaggc ccacatcggc gaacacgcct tccgcgtcgg cacggcacag tatgtcgcca
     5101 ccgggcacgc gctcgacgcc tgggcgcaac gcctgctgga tgaagtcggc agcgaaggcg
     5161 gctccgccgt gctggtcagc cgcgatggcg cgctgatcgg cgccctgcac ctggccgacc
     5221 agatccgcat cgagacgcca cacgccgtgc gcctgctcag acaggccggc atctcgcaca
     5281 tgatcatgct gaccggcgac cggccggatg tggcggagac catcggcatg gcggtcggcg
     5341 tcgacgaaat ctgcgccgga ctcgacccgc aggaaaaact gctgcatatc gcacaggcga
     5401 aatcttccgg catctcgatc atggtcggcg acggcgtgaa cgatgccccc gcgctggccg
     5461 ccgccgacgt tggcgtggcc atgggtgcgc acggtgccgc cgcttcctcg gaggccgccg
     5521 atgtggtact gctggtcgac cggctcgacc ggctggcaga ggcgatccag tttgcgcgcg
     5581 ccacacgcga catcgccgta caaagcgtac tggtcggcat gggactgtcg gtcatcgcca
     5641 tgctggctgc cgccatcggc tggctggcac cattacccgg cgccacatta caggaactga
     5701 tcgacgttgc agccatactc aatgccctgc gcgccctgcg catcgcgccg ctgcacgcca
     5761 gcctggtgcg gctcaatccc gtcgaatcgc gtcgcttgct ggccgaacac gcggcactgg
     5821 ttccgctgct ggacatgctt gaccatctgg ccgaccagct gcccgccctt cctcatgacc
     5881 aggccctgga agaactggct gcgctacact cggcactaca gcagcagttg ctggcccacg
     5941 aacgcgccga cgataaccac ctgtacccga aaatgacacc gctgctcggc ggcaatgatc
     6001 cgctggcagc catgagtcgc acccaccgcg aaatctttgc cctggcacat cgcctcgaac
     6061 gtatcactgc ctcgttgcat ccggatcggg ttaccctgga cgacctgcat gaaatccagc
     6121 gcttgctcta tgcactcggt gcaatcgtcc gcttgcattt tgcacaggaa gatgaactat
     6181 ttttcactct tgctgactaa atgctgactt aaatggacgg atgccgtctt tatgctgaca
     6241 gttcggtcaa actttactac aatgatctca tgcgaacatc ccgccacaac ctgctgatcc
     6301 tgttcgtgct gttctgggtg caatgctttg ccccactgct gcacgcacat gcccatgggc
     6361 aagccgccca gctcgggctg cattggcacg cgaccgatac gctgagcgac ctgcaattca
     6421 gcggccgtgt tcacgccagc ctggttcgtg acaccgacga ctcgacactg atcacgcccg
     6481 agcctgcact gcgccagcac aaccggctgt ctttcagcga tgtggtgcat acggcccctg
     6541 agcaaccgcc ctccgactgc ctgccggtca ccgtaccggt cagcgatccg gcacgatttg
     6601 cctcggctgc tccagccgca acggacaaaa caagaccctt ccggtcacga tacgctcttg
     6661 ctttccttgc acaggctcct cccgtcagcg tctgaatctg tccgatgatt cgaccgcagc
     6721 ggcataggtt gcctgcggcc acacgcttac gaaccaggga gtcaatcatg tctcttgctt
     6781 cacgcatttc cgccgtcatg ctgctcggct gggcgcttgc cgcccaggcg cagacagatc
     6841 tcgtcaccgt caacgcggca caaatccgct cgctcggcat ccagaccgca aagccgcaga
     6901 gcgtttccag ctcgggcggc atcgccctgc cagcgcaggt ggttataccc acccaacacg
     6961 ttcaggtcat cagcgcccca ctggccggcc tcatcgaacg catcgacgtc agcactaatg
     7021 aaaccgtacg tcgcggtcaa cgcctgttgg tgttgcaaag cccggaactg gccggactgg
     7081 agcgcgatta cctccaggcc agcctgcagg cgcaactggc caatcagtcg ctcaaacgcg
     7141 acgaagcgct ctaccgggag ggcatcattg cccagagccg ttaccagcag accgcaagca
     7201 cggccgccat cgctgcggca gcgttgagcg agcgccggca aatgctgcat ctggccgggc
     7261 tggacaacgg cgccatcgca cgcctgcaac gcagccggaa aattgcttcc acacttgaag
     7321 tcaccgctcc gtttgccggg gctgtgctcg aaatcaatgc cagcattggc cagcgcacgg
     7381 acatgacgac cccgctgctc aaactggcga atctgtcgcc gctgtggctg gaaatccagt
     7441 tgccggcaca ccagagccag aacgtgcaga tcggcagcct cgtccgcatc ggatcgggca
     7501 aagccactgg caaaatcatc aatatcggac gcagcgtcaa cccggccaac cagacagttg
     7561 ccgtacgtgc cgaggtcgcc tcgggcagcc acaacctgaa tcccggccag tatgtcgagg
     7621 tgcatatcga tacgccggca ggcaagacgc aatggctgtt gccacccgcg gcaatcgtgc
     7681 accagggcac ccgcgccgtg gtcttcgtgc gggcagacgg ggggttcagg ccagttgcgg
     7741 tgcaggtcac gagccagtcc gccagtggca gtgtggccga agcctcactg aacgcacagg
     7801 acgaaatggt ggtacggggc gccctgtcga tcaagtcgat ctggcaaggc agcggcaagg
     7861 gagaatgaca tgctggaaca catcatccgt ctgtccctca gccagcgatt gctgacactg
     7921 ctgttcgcgc tgctgctggc cgcagccggc tcgctggcct ggctgaaact gcccatcgac
     7981 gcctttcctg acgtgtcgag cacccaggtg aaaatcatca tgaaggcgcc tggcatgacg
     8041 ccggaagaag tcgaggcgcg
//
LOCUS       DAIANL010000160        11171 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2236256, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000160 DAIANL010000000
VERSION     DAIANL010000160.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11171)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11171
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2236256"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2114 a         3184 c         3615 g         2258 t
ORIGIN      
        1 tactgtcagc ccgatactgg gtccgcgttg ccggtatacg ccgacctgtt cccagtacgc
       61 aatcgaggct ctggcacggc atggcgctat acgtggcagc tggctggcca tacgacgcgt
      121 gtcacgctgc catccctacc atcccggcgg atacgatcct gttccatgaa ttttgactct
      181 tgtggctgac ctgcaatgga tgctcaacga ctgatcctgt ctatcatttt ttctctctcg
      241 tcgctgatgt tatggcaggc gtggcaaaaa cacgagcagc catccacgga aaccgcgacg
      301 gtgacggcac cggtcaaggg caatccgtcc acggttggcg cgggcgcacc ggttcccggc
      361 accccgcagt ggaacagcgc caatactccc gcagccgcac cagccagtgc gtttcctgtc
      421 ggtccgcagg cgatagtcag taccgacctt tttgatgcaa ccatcaatgc tgctggtggt
      481 gatatccgta acctgacgtt gaagcaatat ggcgatacca cccgccccgg acaccatctg
      541 catctgttcg aacagcaacc ggatcacctg ttcgttgccc agtcgggtct ggttggaccg
      601 ggcttgccaa cgcacaaatc gatgttcacg ctgcagccgg ggaatcatga gctgcaaccg
      661 gggcaggaca cgctgtcgct ggtgctcaac tgggatgacg cccagagcgg catgcaggtg
      721 cagaagacct atgtgtttca ccgcggcagc taccgtatcg atgtgttgtg gaacatcgtc
      781 aaccgcagca ataccaatgt gagcgtcgac gcgtattatc agtttgtgcg tgatggcaag
      841 ccgcccgtgg gtgagtcgcg cgtggtgcat acgtttacag gcccggccat ctatacccag
      901 gcaagcaagt tcgtcaaact ggatttccac aaactggaca agggagagca ggagtacccg
      961 aaatttgcca ataatggctg gctggccatg gtacagcacc attttgtctc ggtgtggctc
     1021 ccgcccaatg gcggcaatcg cgaatttttt gcgcgcccgc tgggcgatgg gctgtacagc
     1081 gccggtgtga ttctgcctga aggacgagtg gctccggggg gctcgctacg tctcgacgtc
     1141 ccgctgtatg ccggtccgca gatccagaag gtgctgacag cgcttgcgcc cgggctggat
     1201 tacgtggtcg attatggttg gctgacaccg atcgccgtgc cgattttctg ggtgctgcag
     1261 cacatccatg cctgggtagg caactggggc tgggccatcg ttattctgac cattggcatc
     1321 aaattgctgt tttttccgct atcggccaag agctatcgtt cgatggcaca gatgcgcggg
     1381 ttgtcgccca agctgcagcg cctcaaggaa caatacggcg atgaccgtga gaagttgcat
     1441 ctggcaatga tggaattgta caagaccgag aaggtcaatc cgcttggcgg ctgtctgccg
     1501 acggtcgtac agattccggt gttcatcgca ctgtactgga cgctggtcgg gagtgtggaa
     1561 atgcgtcagg cgccgtttat cggctggatt catgacctgt cggcacccga tccattctat
     1621 gtgctgccga tcatcatggg tttgaccatg ttgctgcaga cccggctcaa tccggcgccg
     1681 cccgatccca tgcaggcaaa gatgatgatg atcatgccgg tggcatttac tgcatttttc
     1741 ctgttctttc cgtccggcct ggtgttgtac tgggtggtga acaacaccat gtccatcctg
     1801 cagcaatggg tgattacgcg caaattcgat gcgagccggg cgcgctgagc gtttggctga
     1861 ccgcaccgat gtgtgaccgg ggtgaacagc atgatccccg tgtctgatac catcgttgcc
     1921 atagctacgg cagccggacg tagcggtatc ggtgtggtgc gcgtgtccgg tcccggggtg
     1981 tgcgagtggc agcatccctt gttcgggcgt ctccttccac cacgacaggc gctgctgtgt
     2041 acgctgcgtg atgagcacgg cgaggccatc gatcaggtgg tggcgctgta ctttgcggca
     2101 ccggcttcct acactggcga ggacgtgctc gaactgcacg gtcatggcaa tccggtcatc
     2161 cttcaggcga tagtacagcg ctgtgtgcaa ctgggtgcgc ggcttgcgcg accgggagaa
     2221 ttcaccgagc gtgcctttct caacgacaag atcgatctgg ctcaggctga aagcgtggcc
     2281 gacctgatcg atgccggatc gatcgaagcc gcacgatgcg cgatgcgttc gcttgaggga
     2341 gagttttccc gcctgatcgg tcagttggcg caggaactgc tcgaactgcg tatccaggtc
     2401 gagtcgatgc tggattttcc cgaagaggtg ccggaatacc tgcatgcgct gcgcattgtc
     2461 gaacgtctgc aatcgttgtt gcagcgcttg cgccagattg agcaggccgg acggcagggc
     2521 agtctgttgc gcgagggcat ccaggtcgtg ctgatcggcc agcccaatgt cggcaaatcc
     2581 agtctgctta accggttggc tggaaatgaa cgggccatcg tgaccgatat tgccggcacc
     2641 acgcgtgata cgttgcgtga aacgattgtg ctacacggca tcacgctgca tatcatcgac
     2701 acagcgggct tgcgcgacac cagtgacccg attgagaaaa tcggtattga gcgtacctgg
     2761 caggccattg gccatgccac actggcgctg ctgctggtgg atgcgcgtca gggcatcact
     2821 gcggccgatc aggccatcat cgaccgcttg ccgcccggat tgccacaatt gctggtgcac
     2881 aacaaatgcg atttgcctgg tgccgatgcc atggcgtcga ctgtcggcgt gtcggtatcg
     2941 gcgcgtaccg gccatgggct ggatgtgttg cagcaacgcc tgttcgaatt ggcgggctgg
     3001 cgcggcaata ccgaaggcat ttacatggcg cgcgaacgcc agttgcaggc gttgcaggcg
     3061 acgcaacgcc acgtgaccca ggcgcttgat cagcttgcca ttgccgaatg ggtggccgag
     3121 gaattgcgtc tggcccacat tgcgctgtcg gcgattaccg gtgcggtcag cgcggatgac
     3181 ttgcttggcg aaatcttttc acggttctgc atcggcaagt gactccgcgc ggggttactg
     3241 tgccggctcg aatcgcagca cagcgctgtt gatgcaataa cgtttacctg tgggtggcgg
     3301 tccgtcatcg aacacatgcc cgaggtggat gcccgtgctt gcactcagca cttcgacgcg
     3361 atgcatgcca tgactaaagt cgtcgtgcat gatcagcgcg ccaggcaccg gcgctacaaa
     3421 gcttggccaa ccgctgtggc tttcgaattt tgcgtcgctg cggaataacg gtgcgccact
     3481 gagcgggtcg acataagtgc cggcctcatg gttgtgcagc aacaggccgc aaaacggcga
     3541 ttctgtagcg gcgttgaggg caatgtcgcg ctgttgcggc gtcagcatcc attgcgccag
     3601 ccagcgccag aattcgtaag cctgaccgtc ataaccatgg cgccggctga tttcttcacc
     3661 attgcgacac agcacaaaca ccaaaccggg acctggcggc tgatgcaaca cgcaacccgc
     3721 gggagcggcg cccctgtcga cgcggatcag cggtaatggc gagcgccagc cagcggcgag
     3781 ttcctggtcg aagcgctgac aatcagggca ggcatcaggc tctattgcca gcaactgtag
     3841 cggcgccggc gatgattcgg agagcaatgc cggtgtcgtc gccgacaccc cgctcgggta
     3901 aggcacaccc gtaccgcccg cgccacaata gccgttcgga tgcttgacca ggtagtcctg
     3961 gtgccaggcc tcggctggat agaactggcg cagcggcgtg atttctgtgg tgatgcgtcc
     4021 gtagcctgcc gcagtcagtg ctgtctggta ggtctggcgg gtgcgctcaa tcaccggcaa
     4081 ctgggcggcg ctggtgtaga aaatcgcgct gcgataattt gcgccgatgt cattgccctg
     4141 acggttgcct tgcgtcgggt cgtgattttg ccagaatgca accagcacac gttcaagcga
     4201 gatgcgtgcc gggtcgaagg tcacgcgcac cacttcggcg tggacgggtg cgttgccgcc
     4261 ctggcgcgcg tctgcgatga ccgattcgta gtccggcgcc gggcggcttc cgccggcata
     4321 accgacatcg gtgtgggtta ctccgggcaa tgcggccatc cgatgctgcg ccccccagaa
     4381 gcaccccata ccaagtacga tggtttcgtt gtgcgccggg gcgccatgag cggagggtgc
     4441 catcatccag ggtaaggtac acaataaaac agcgggccat cggtgcatgg ttggtctcct
     4501 gggttggatg ggtttcacgt gaaacagggt ggctgcgggg caggggttca cctgaaacga
     4561 tgccggacat acaaaaacat ttcgtctggc ggatatttgg tacaatacgc ggttcgaatg
     4621 cgtcagggaa gagggaattg ccgggcgcgg cgttgcggaa cggaaagacg catgacatac
     4681 agtgaatcgt tcgatgtgat cgtggtggga ggagggcatg ccggaaccga ggctgcgctg
     4741 gcggctgcgc gcatgggcgt gcgtacgctg ctgctgaccc acaatcttga aaccctcggg
     4801 cagatgtcct gcaatccttc gatcggcggc atcggcaaag ggcatctggt gcgcgaagtc
     4861 gatgcgctcg gcggtgccat ggcggctgcc accgatgaag ccggcatcca gtttcgcacc
     4921 cttaattctt ccaaaggcgc tgccgtgcgc gccacccgtg cacaggccga ccgacagttg
     4981 tatcgccagg ccattcgtgg ccgcattgaa aatcagccga atctgcgcgt atttcagcag
     5041 gccgtggacg atttgctggt tcagggggag cgcgtgattg gcgtgcgcac gcagttgggg
     5101 ttggtgttcc atgctccgaa cgtggtgctg acaagcggta cgttccttgc cggactgatc
     5161 catgtggggc tggcgcatca tgccggcggt cgcgccggcg agcccgcggc ggaaacgctg
     5221 gctgcgcgtt tgcgggaact gcagttgcct gcgggccggc tcaagaccgg tacgccgacg
     5281 cgcatcgatg gccgcagcat cgactattcg gtcatgcacc gccagccggg cgatgatccg
     5341 ccaccggtgt tttcctttct ggggcggcgc gaacagcatc cgcgccagtt gccttgctgg
     5401 atcacccata cctcacagcg cacgcacgat atcattcgcg gcggtatgga ccggtcgccg
     5461 ctgtataccg gagtgatcgg cggagttggg ccgcgttatt gcccatcgat cgaagacaaa
     5521 gtggtgcgat ttgctgacaa ggattcgcac cagattttcg tcgagccgga agggctcaat
     5581 acccatgagg tgtatcccaa tggcatctcc accagcctgc cgttcgatgt gcaatgggcg
     5641 ctggtgcgat cgattcgtgg tttcgagcag gccaatctgc tgcggccagg ctatgccatc
     5701 gaatacgact attttgaccc gcgtgccttg cggcgctcgc tggaaacgcg cgtcctgtcc
     5761 gggctgtatt ttgccgggca gatcaatggc accaccgggt acgaagaggc tgcggcgcaa
     5821 ggaatgctgg ccggtattaa cgccggtctg gccgcgcgcg ggctggagcc gtggcatccg
     5881 gggcgggaac tcgcctacct gggggtcatg gtcgacgacc tgaccacgct gggcgtgacg
     5941 gagccgtatc gcatgttcac cagccgcgcc gaataccggt tgtcgttgcg ggaagacaac
     6001 gccgatctgc gcttgacgga agtcggccgt cagctcgggg tcgtggatga tgtgcgctgg
     6061 gaagcgttca accgcaagcg tgatgccatt gcggctgaaa gtgagcgctt gcgtcaggtg
     6121 tgggtacgac cagagacgtt ccgaccggtc gacgccgaac gcattcttgg tcagcagatc
     6181 gagcgcgagt acaacctgta cgaactgttg cgtcggccgc aggtgagtta tgccgaactg
     6241 atgacgctgc cgcaggccgg cgaaggcgtg actgacgaag cggtggcgga acaggtggaa
     6301 atccaggcca aatatcaggg ctacatcgag cgtcagcagg acgaaattgc ccgcatgcgc
     6361 ggccaggagt cgctgcgtct gccggaagac atcgattaca tgagggtggc cggactgtcg
     6421 attgaagtgc ggcagaaact gtcgcgtcag cggccagaaa cgcttggaca ggccgcgcgc
     6481 atttccggcg tgacgccggc ggcgatctcg gtattgcagg tacacctcaa gcagctgggc
     6541 gcgccacgtc gggacgcgcg ggcatgaagc aacccgccga cctgcgcgcc gctgccggca
     6601 cgctcggctg tgatctcgac cagatgcagg cggagcgcat gctggaatac ctggcgctgc
     6661 tgcagaaatg gaatcgggtg cacaatctga ccgcggtgcg cgacacggat gatatgttcg
     6721 tgctgcactt gctcgatagc ctggcggtaa cggcctggat ggagactgca ttgttgctgg
     6781 acgtcggcag tggtgggggc ttgcctggca ttccgctggc aattgcccgg ccagccatgc
     6841 aggtggaact gatcgatagc aatctgaaaa aaaccagttt cctgcgtcag gccgtgatcg
     6901 agctcggttt gcgcaacgtc accgtccgca gcggccgcgt cgagcagctt gacggccgtg
     6961 ggcgctatcc aggcatcatt gcacgcgcat ttgccgaaac gggcgagctg gtgcgactca
     7021 cgcgggcccg gcttgcgccg catggacgct ggtatgtcat gaagggcgcg tatcctgaag
     7081 ccgagttacg tgagttgcca ccgggggtgg tggtggaatc ggtacacgcg ctcgatatac
     7141 ccggcctgcg ggccgaacgg catttgctga ttttgaagga tgaccatgag caggatattt
     7201 gcgatcacta accagaaggg cggggtgggc aagacgacca ccgcggtcaa tctggcggcc
     7261 gggctggcag tacgtgacta ccgcgtgctg ctggtcgacc tcgacccgca gggcaacgcc
     7321 accatgggtt gcgggatcga caagcgtgcg ctggatgcca gcgtatacca cgtgctgctc
     7381 gatctgcgcg gtgtgcgcga tgccatccgc cgcagtgaaa gcggaggatt cgatgtgttg
     7441 cccgccaatc gcgatctggc cggcgccgaa gtcgaacttg tcgatcagca gcagcgcgaa
     7501 ctgcggctgc gcaatgcgct ggatgtgctt ggcgatgatt acgattatgt gcttatcgac
     7561 tgtccgcccg cgctcaacct gctggcagtc aatgcgttga ctgcggccga tgcggtgatg
     7621 atccccatgc agtgcgaata ttatgcgctg gaaggcgtgt cagacctggt caataccatc
     7681 cgccgcatcc gccagcattt caatccgaag ctggaaatcg aagggctgct gcgcacgatg
     7741 tacaacccgg gcagcacgtt ggcgcaacag gtgtctgacc aattaaaaaa ccattttgaa
     7801 tcaaaagtat atgacaccgt cattccgcgc aatgtgcgtc tggcggaggc tccgagttac
     7861 gggcagccgg tgttgatgta cgacccatcg tcgaaagggg cgcaggccta tcatgccttg
     7921 gcgcaggaaa ttgtcacgcg cgctgcacaa gccggaaact gagaggaagc aatgaaaaaa
     7981 atcagggggt tgggtcgcgg gctcgatgca ttgctggggg gtgacgaacc ggaaaccgct
     8041 tcgggcacgc tgcagatggt cgcgatcgac cggctgatgc cgggaaaata tcagccacga
     8101 acacgcatgg acgccgaaag cctcgaggcg ctgtcgcagt cgatccaggc acaaggcctg
     8161 atgcaaccga tcctggtgcg cagcctgccc gacgaccggc tggagatcat tgccggcgag
     8221 cggcgctggc gtgcggccgg catggccggg ctggcggaag tgccggtgct ggtgcgtcag
     8281 gtggctgatg aatccgcgct tgcaatggcg ctcatcgaga acatccagcg ggaaaacctc
     8341 aacccgctcg aggaagctgc cggtatccag cggctcatcg acgaattcgg catgacccat
     8401 caggacgcgg cgcattcggt cggtcgctcg cgcagcgctg tatccaacct gttgcgtctg
     8461 ctgggactgc ccgcggcagt gcaggcgctg atgatggatg ggcagcttga catgggacac
     8521 ggccgcgcgt tgctggctct gccggaatcc tcgcgcgaga gtgccgcgca ccaggtggtc
     8581 gaacgcaacc tgagtgtacg agaaaccgag cgctgggtgg cgcggctgtt gggcgagccg
     8641 caggcgcagc acaagccgcc agcgccagtc gatcgcgacg tggccgacct ggccgaacgc
     8701 ctgtcagacc gcctgggtgc gcaggtcagc attcgcggcg ggcgcaacgg ctcgggcaag
     8761 ctggtgatcg aatacgcaag cctcgatcaa ctggaccaaa taatcaacag gttgtaagca
     8821 tgaaatccgc ttcttgacag gtaaatcgga catttatgtt gcaaaaggac atttgagtca
     8881 tgtatcatct cgccgattgt gcggagcagg cagcccttgg cacgacgtgc ccgggaccgc
     8941 aaacaacgca aaaaaacgga tgtggctcat gaatatgccg tctgcggaac ctggctgggc
     9001 cggtgcgcgg cgggtcatca ccctgcaggc agtggtggtg tcggcgggag ccgtcctggc
     9061 ggccgtgctg cacccggaag ccatccgcgc cgtgctgtat ggcggcctgg tggcggtgct
     9121 gggcagtctg acgctgttct ggcggctgag ggcgacacaa cagcgcgcgg gtcttgatgc
     9181 acaccgtaat ttgcgtatgg cgcaacagac gtccgcagca cgcttcgtgc tcacggtcgt
     9241 tctgctggcg ctgccgctga cacaccgtgc gcactggcca tatgcctggg tcgtcacggg
     9301 gtttgtggca ggacagataa ccggactgat cgggctcgcg ctcgggcaga tccgcaagaa
     9361 cggaacatga cagggttggc ataacaacat ggctacggaa acgcttacgt cgtcggaata
     9421 catccggcac cacctgcaga atctgacctg gggccgcttg ccggatggcg ggttcggtat
     9481 tgcacagaac gccgagcagg cccatgcgat gggcttctgg gcgatcaacc tcgacaccat
     9541 gttcatgtcg ctgctgctgg gcgtggtgtt cctcgggttg tttcgcagtg tggccggatc
     9601 catgacggcg ggcattccgg gtggcgtgca gaatttcgtc gaatggatcg tcgaattcat
     9661 cgattcgagc gtgcgcggct cgttcagcgg gcgcaacccg atggtcgctc cgctggcgct
     9721 gaccatattc gtgtggattt ttctcatgaa cctgatggac ctgctgccgg tcgactgggt
     9781 gccgcacatc gccaaggcgc tgggcgtacc cttcttccgt gtggtaccga gcaccgatcc
     9841 caacggtacg ttcggcatgt cgatcagcgt gttcctcatc gtcgtgttct tcagcatcaa
     9901 ggtcaaaggc ctgggcggat tcctcggtga gcttgcattt cacccgtttg gcaagatcgg
     9961 cttgcccgcc aacctgttcc tggaaggcgt caacctgatc gccaagccgg tatcgctggc
    10021 gctgcggttg ttcggcaaca tgtatgccgg cgaaatgatt ttcatcctga tcgcactgat
    10081 gtttggtggc ggatacgggt tggccgcatt tggcggtgcc ctgcaatggg catgggccgt
    10141 gttccacgtg ctgatcatca cgctgcaggc gttcattttc atgacgctga ccatcgtata
    10201 catggacatg gcgcatcacg tcgaggatca ctgagaagct gcgcatgcgg cgcggcggac
    10261 aagttgaacg gtttggattt tgatttttaa tcttgaggag cagtaaatgg atatggttca
    10321 tgcagtgttg tacatcgccg gcgcagtgat gatggggctg ggtgcactcg gcgcctcgat
    10381 cggtatcggt attctcggtg gtcgctttct cgaaggcgcg gctcgccaac ccgaattgat
    10441 tcccatgctg cgcacccagt tcttcatcgt catgggtctg gtcgacgcgg tgccgatgat
    10501 tgccgtcggt ctggccatgt acgttctgtt cgctgtggcg ggttgatcgg tcaagtattg
    10561 aaaccgtgag agaggttcca gcatgaacat caatgcgacc ctgatcggcc agtccataac
    10621 gtttttcgtg tttgtctggt tttgcatgaa atacatctgg ccgccgatca tgaatgcgct
    10681 gcacgagcgc aagcagcaga tcgcagacgg cctggcagcg ggtgaacgcg gacggcacga
    10741 actggagctg gcgaccaagc gcgccacttc ggacatgcac gacgccaagc tcaaggccgg
    10801 cgagatcgta gcccaggccg aaaaacgtgc gacgcaggtc atcgaggaag ccaagcaggc
    10861 tgcccgtatc gaagctgacc gcattctggt cagcgcccag gccagcgtgg accaggaagt
    10921 ggtgcgcgcc cgtgaagcgc tgcgcagcca tgtggccgaa ctggcggttg ccggcgccga
    10981 gcgcatcctg cgtcgcgaag tcgacgccaa ggtgcacgcg gatctgctga ccgcgatcca
    11041 gaacgagctc tgatcatggc cgaaatcgct accatcgccc gtccctacgc tgaagcggcg
    11101 ttcgacattg cccgcgaatc ctctggcgcc atcgatgcct ggtcggagca gcttgcgctg
    11161 gccgcccgga t
//
LOCUS       DAIANL010000161         5891 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_3265842, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000161 DAIANL010000000
VERSION     DAIANL010000161.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3265842"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1242 a         1938 c         1630 g         1081 t
ORIGIN      
        1 tgcaaagtca cggaaaaagc cctgcgccag gcatcctgtg tgtttacctc tgcccgcgaa
       61 ggcatcatga tcaccgacag gaacggcggg atcctcgatg tcaatgccgc cttcacccgc
      121 atcaccggct acgagcgcga cgaaatcatc ggccgcaacg ccagtctgtt ccagtccggg
      181 cgtcacgacc aggaattcta cgcccggctg tggcacgatc tggaaaagaa aggacactgg
      241 tacggagaaa tctggaaccg gcgcaaaaat ggcgaaatct acgccgaacg cctcaccatc
      301 agcgccgtgc ccgacacgca gggtgaacag cgccagtatg tggccatgtt ttccgatatc
      361 acggcgatca aggaccacga acagcagctg gaatacattg cccactacga tccgctgacc
      421 ggattgccca accgccgcct gctcggtgac cggttgcgtc aggccatggc ccaggcacag
      481 cgccacgtcc agccgcttgc gctggtgtat ctcgatctcg acggtttcaa gaccatcaat
      541 gacctgcacg gccacgacac cggagacaag ctgctgatcg ccctggccgc atccatgaaa
      601 caggccctgc gcgaaggcga cacgcttgcc cgtctgggcg gagacgaatt tgtcgccgtg
      661 ctgcccgaac tgtccgacgt cgaagcctgc ctgcccatac tctcgcgcct gctcagcgca
      721 gcttcccgcg aagtgaccct ggcccacagc aaccgcatga tacgagtgtc agcaagtctg
      781 ggagtggcac tctacccgca aagcgaagaa gtcgacgccg accagctgtt acgccaggcc
      841 gaccatgcca tgtaccaggc caagctggcc ggcaaaagcc gctatcattt cttcgacgtc
      901 gagcatgacc gtaacctgcg tggccatcac gaagcaatcg aacggatcgg ccaggccctc
      961 gccaacaacg aattcatcct gtattaccag cccaaggtca acatgaaatc cggcgcactt
     1021 atcggtgccg aagcgctgat ccgctggcaa catccagaac gggggctgct gacgcctggc
     1081 agcttcctgc cggtcatcga agagcatccg ctgatcatca cactgggcaa ctggacgctg
     1141 gatcgcgcgc tggcacaaat cgacgcgtgg cagacaacag gcctgaacct gcccgtcagc
     1201 gtcaacatcg ccgcacgcca cctgcagcga ccggatttca tggaccaact gcgcggcgtc
     1261 ctcgcacgct atccggatgt gccgccctcg ctgctcgaac tcgaagtgct tgaaaccagc
     1321 gccctggaag acatccgcca cgtatccgag gtcatccagg actgcaggcg cctcggcgta
     1381 tcgtttgcac tcgacgattt cggcaccggt tattcctcgc tcgcctatct gcgccacctg
     1441 ccggtccagg tactcaagat cgaccagagc ttcgtacgcg acatgctcga cgaccccgac
     1501 gaccttgcca tcctcgacgg cgtgctcggc ctggccagcg ctttcaatcg ccttgccatc
     1561 gctgaagggg tggaaacccg cgagcacgga gaactgttgc tgttgctggg gtgcgaagtc
     1621 ggccagggtt acggcatcgc acatccgatg ccggccatgg atctgcctgg ctggcaggcg
     1681 caatggcagc ctgcagccag ctggcgcagt cgctcaagcg cgcaccgcag cagcctgccc
     1741 attctgtatg ccgaggtcga acacagggca tgggtcacgg cagtagaagc cagcctgaac
     1801 aaccgtggcg gcgaaccgcc tgaacttggc gagcacgact gccgcttcag ccagtggctg
     1861 gccggtgata tccgacagca cgacccggag ctcatcgcca gcctgacgca tctgcatcgc
     1921 agcgcacatg actgcgctgc cgaactgctg gccatcaagg cgacgggaga cgtcgatcag
     1981 gccactgccc ggctcggcga actgtatggc atgcgcgaca gcatgatcgg catgctgcgc
     2041 cagctgatcc actgacggac atgcttctgc gcggccctac ccggctgcgt attcccccag
     2101 cccgtcgagc agcacaccca gatgacgctc gtagttgcgc catgcctgcg aactgccctg
     2161 atacatgccc cgacgcacct gagtggcgct ggccgttcgc accgggcgcc gggtctgttc
     2221 aaatgacaga cagcggtcat tccaggataa cccgcagaag tcgagcaatc gacgggtctg
     2281 cgcttcctga tctacggtca gtcgttcgta atccagatcg cagatgcgtc cgggaaaaca
     2341 ttcatgccag aacgccatga gttgccggta catgcggtag tactctgcaa gttcggccag
     2401 atcgcaggca aatcccagcg tgcgctgggg aaaaaactgt ttgaacaacg accagcacgt
     2461 cgccaccgga tcacgcatca ggtgcaccac cctgacgtcc ggattcgtca gcagcaacac
     2521 gccgagccag cggaaattgt gcggcatttt gtcggtaaca cagatctggc cgggcgcaat
     2581 ctgctgcagg gcagcctgat agcggctggc aatttcctgg ctgacctgct gcagcgacgc
     2641 cggccgggca gcaagaaaat gctgccccac cagggtgttc agcacttcaa gttcaccggc
     2701 gccatacacg tcaggatgcg aggccagaat ctgttccacc agacttgtac ccgaacgcgg
     2761 catgcctacg acaagtatgg cacgcggttg accggtcacc acgggctgcg cggacggcaa
     2821 cggcgtgaat gctgctcgga tagctgcaaa cagcgtccgg tcctcgtcga tgacgtaacc
     2881 ggattgctgt cgcagcagtc ggttaccttc gacgtacagc ccgaacgcgg tatcgaactc
     2941 accgatctcg tcactcgccc gcgccagcgc gaagcaggca tgggcacgtt ctgccgggtg
     3001 agtgactgtg ctgtacaacg cacgcaattg cggcaagcgc ggatcgtcgt ggacacaggg
     3061 tttcagttca cccagggcac ggtgacagtt cgcatcgccc ggattgagag ccagtgcccg
     3121 ctcgtagctg gcgatggcct cgtcaatgtg cccgagatcg cgcatgaccc agcccaggtt
     3181 gcaatgcgcc tgttccagcc cgggtttcag ttcgatcgcc cgtcgataac tggcggccgc
     3241 cgcttccagc ttgttcagct gacgcaatcc gttgcccaga ttgtaatgcg cctcggcatt
     3301 gtcaggatca agcgagagcg ccgcccgact gcatgcttcc gcctccgtcg cgtggcccag
     3361 ttcgagcaga atgaccgcca gtgcattgtg tgcttcggca tcgtcgggct gcaggcgcac
     3421 ggccactcgc attgcctgcg ccgcctcggc attccgcccc agcaggtgca gcgcgatgcc
     3481 gagggttttc cagccaaacc catcctccgg acaggattgc gtccattcac gagccagttc
     3541 tgcggactgg ccaaactggc cgctgcccaa cagcctgagc aaggtgtttt tatgtgtttc
     3601 cgggatcgtc accgctgcgg cataacgccg cagtactgcg gcgatctgcg caaggtcagc
     3661 ggcagccagc ccctgccgtt gcgcccgcgc aaacaggatt tcaccgcccg cgacatcgcc
     3721 gacggctcgc agcatttgcg catgactcaa ccagaactgg agttgcgttg gcgcggcgga
     3781 aaccgccatt ccaagcaggt tcagcgcctg ttcctgtcga ttggtctgcg ccagcaatat
     3841 gcccagatta tggcaggcat cgggttgctc gggccagcga ctcaggatat cgcggtagcg
     3901 caattccgct tcgtccagct ggcctgcctg atgcagactg atcgcctgcc gcaacgcctc
     3961 ttcgcaggca atcgttgccg cgccccccga aacactggca tcatgcatat cgcatgccca
     4021 tcgcatcatc cggcagcacc accaaccacg cccggaaaac gcaaacggaa ttgcagtggc
     4081 cagctcgcga aagcattcgc aacgttgatc cgccaccgca aacaccagac gctggttgat
     4141 gaaaagtcat attgaattgt tgcatactga attgtttcat tgtcagacca tcatacggag
     4201 aattcgtcat gcacgccaga catctgcctg cctcaacact cgtcgccgca ttgttcgcca
     4261 ccggtctcgc agcgcacgcc atggagccgc tgccggccaa ggttccggtt ccggccgata
     4321 accccatcac tgccagcaag gtcgaactgg gaaaagagct gtactttgat ccccgactgt
     4381 ccgtcgacgg caccgtgtcg tgcaactcgt gccacaatgt gatggcgggt ggcaccgaca
     4441 accgtccggt ctcggtaggc gtgggcggga agaaaggcag tcgcagtgcg cccacggtat
     4501 ggaattccgc ctacctcacc gcgcaattct gggacggtcg cgcgcccacg ctggaagaac
     4561 aggcaaaagg gcccatcctc aacccggtcg aaatgggcat gccgtctgcc gatgtagtgg
     4621 tggcgcgcat caagggcatt cccggctacg ctgaagagtt caaatccgtg ttcggtggca
     4681 aggacccggt cacctacgac aatatcgcca aggctatcgc cacctacgaa cgtacgttgg
     4741 tcacccgcaa cagtcccttc gaccgctaca tgcacggcga caagcacgcc ctgtcggccc
     4801 aggcgcagcg tggcatgaaa ctggtcgagc agatcggctg caccgcctgc cacatgggcc
     4861 ccaatttcgc cggaccgaca gccctgccga tgggccaggg tttctatcag aaatttccgc
     4921 tcaataccga caatgattat gtgaaaaaat accacctgga agatgatctg ggacgctacc
     4981 aggtcaccaa aaacgacaac gataaacaca tgtggcgcgt gcccacctgg cgcaatgttg
     5041 cgctgaatgc cccctacttc cacaacggat cggtgccgac gctcgacgag gccgtgcgtg
     5101 tcatggcccg gacccagctc aacaccacgc tgaccgatgc ccaggtggcc gatatcgtga
     5161 ccttcctcaa ttcgctgact ggcgaatttc ccaagcagac tctgccgcgc ctgccggcca
     5221 caaccggcag ttcccctgtc gccgacaagt aaacgcacct tatgaaaaag cacccttcgg
     5281 ggtgcttttt ccggaattga atcgcttgcc cgttggcggc tgacggataa tggttcctgc
     5341 tacacccagg caacacgcca gtttgccatg catgatggcc gggttatttt tttccaagaa
     5401 tttccttgag gatgtcgccc ttcagccgtt cctcggcgta catcttcttc agccgggcgt
     5461 tctcggcttc cagttccttc agacgtgcca tcatgggtac gtcaagtccg ccgtacttca
     5521 cacgccactt gtaaaacgta ccatcttaag gaaactctgc aaaaccactc acttctgcgc
     5581 cccgcacccg agcccattgt ggcaatatta agcccatgaa acaaactacc ctgaacctcg
     5641 acctcagcct caagaagacg cgcaagcgcg agtttctcga gcagatggaa cacgtcgtgc
     5701 cgtgggcagc gctggtcaag ctgattgccc cgcactatcc caaaggcacc aatagacgcc
     5761 cgccgttccc gctcgaaacc atgttgcgca tccacttcat gcagcaatgg ttcagcctgt
     5821 ccgatccggg catggaagaa gccttcttcg acgtgccgct gtaccgcgaa tttgcccaac
     5881 tcgaagacta t
//
LOCUS       DAIANL010000162         5872 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2037710, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000162 DAIANL010000000
VERSION     DAIANL010000162.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2037710"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1196 a         2028 c         1684 g          964 t
ORIGIN      
        1 ctcatgccga tacggaacgc atcggcaaca taccacagac gctccggtcc cggattgcca
       61 atctcggcct agatcacgct gcgctcgcgc gtgagcggat ccaggccatc acgaccggtt
      121 tccaggccac gcaacgcctt ctgcaacgat tcgcggaacg tacgcccgat cgccatcact
      181 tcgcccaccg acttcatctg tgtggtcagg cggtcattgg ccagggggaa tttctcgaag
      241 gcaaaacgcg ggatcttggt caccacgtag tcgatcgacg gctcaaacga tgccggcgtg
      301 acgccgccgg tgatatcgtt ggtcagttcg tcaagcgtat aacccacagc cagccgcgct
      361 gcgactttcg cgatggggaa gccggtcgct ttggatgcca gcgccgacga acgcgacacg
      421 cgcgggttca tttcgatgac caccatgcgg ccgtcagccg ggttgatgcc gaactgtacg
      481 ttcgagccgc cggtttcgac gccaatctcg cgcagcacgg cgatcgatgc attacgcatc
      541 aactggtatt cgcggtcggt cagcgtttgc gacggcgcaa cggtgatcga gtcaccggta
      601 tgcaccccca tcgggtcgag attctcgatc gagcagacaa tgatgcagtt gtccttgcgg
      661 tcacgcacca cctccatctc gtattctttc cagccgatca gcgattcctc gatcagcaat
      721 tcgcgcgtgg gcgaggcctc aagtccgcgt tcgcagatct cgatgaattc cgcacggtta
      781 taggcgatgc cgccgccggt accgcccatg gtgaacgacg gccggatgat ggtcggaaat
      841 cccatcactg cctgcacctg cagggcttct tccatgctgt gcgcaatcga cgaacgcggc
      901 gagtccagcc cgatgcgcgt catcgcctgc ttgaattttt cccggtcttc cgctttgtcg
      961 atcgcgtcgc gcgaagcgcc gatcaactcg acgtcaaaac gttccagcac accattgcgc
     1021 gccaggtcga gcgcgcaatt gagtgccgtc tgcccgccca tggtcggcaa cagcgcatcc
     1081 ggacgctcgc gctcgatgat cttttccagc accgtccagg taatcggctc gatgtaagtc
     1141 acatctgcca ttcccggatc ggtcatgatg gtggccggat tggaattgac gaggatgatg
     1201 cgatagcctt cttcgcgcaa ggcggtgcag gcctgggccc cggaataatc gaattcgcac
     1261 gcctgcccga taacgatcgg acccgcgccg atgatcagga tgctgtttat gtctgtacgc
     1321 ttgggcattg ggcaccaggt gtggattgta tggtcaaacg gccggctgcc ggcgtgcacg
     1381 catggtggcg acaaaacggt cgaacagtcc ggccatgtca tgcggcccgg ggctcgcctc
     1441 ggggtggccc tggaaactga acgccggcgc atcggtgcgc tcgatcccct gcagcgagcc
     1501 gtcgaacagt gacacatgcg tcgcgcgcag cgtgtgcggc aacgtcgcgg catcgacggc
     1561 aaaaccatgg ttctggctgg taatcatgac ccgaccggta tcgagttcgc gcaccggatg
     1621 gttggcgcca tgatggccga atttcatctt gacggtcttc gcgccggaag ccagcgccag
     1681 caactggtgc cccaggcaaa taccgaacaa cggaatgcct gcgtcgagaa catgacggat
     1741 ggcatcgacg gcataatcgc acggttcggg atcacccggt ccattggaga gaaacacgcc
     1801 atcaggctgc agagcgagcg cttcctgcgc actggtctgt gccggcagca cagtcacgcg
     1861 gcagccgcgt gcggccagca tgcgcaggat attgcgcttg atgccataat cataggccac
     1921 gacgtgcagc ggctgcgcgg cgggagtcac gaatccgcgc cccagcgccc actcgccctc
     1981 gcgccattcg tagcgcgcct tgaccgacac ttccttcgcc agatccatgc cggccaggcc
     2041 gggaaaggct cgcgcctgcg ccagcgcctg cgcctcgtcc agagtgccct cagccaggat
     2101 acaccctgcc tgggcaccgt attcgcgcag atgccgggtc agccggcgag tatcgatgcc
     2161 ggcaatggcc accacgccat tgcgcaccag atgatcctgc aaactcgact cgctgcggaa
     2221 attgcttgcc agcagaggca ggtcacgcac caccagcccg gctgcctgca acgccgagga
     2281 ttctgcatcc tcggcgttca cgccggtgtt gccaatatgc ggataagtca gggtaacgat
     2341 ctgccgggcg taagaaggat cggtgagaat ctcctgatag ccggtcatgg cggtattgaa
     2401 aacgacttcg ccaacagcca cacccggcgc tccgatggaa acgccccgga acaccgtacc
     2461 atcagcgagc accagcagag cggaatacgt ttgcgacaag gaaaattccc cggaatcaag
     2521 cggctgcaaa ataacaggcc aaaagaaacg ggccagggtt tacaccgcgc ccgctcctgc
     2581 aacaattgcg tcattttagc gcaaacgggg cgggttgtcc aaccgcagcc agtcccggca
     2641 cggcgacagg atcagctccc gttcctgtcc ggggaaccac ccttgccgcg ccgcagcgga
     2701 taccaggcca acgggatcac gaacatcgac atcaatggcg cagtcagcat cccccccacc
     2761 atcggcgccg cgatgcggct catgacctct gaacccgtac cgccaccgag cagcacgggg
     2821 agcagtcccg ccagaatgac cgctgcggtc atcgccttgg gtcgtacccg ctgcactgcc
     2881 cccgcgcgca cggcagccag cagccccgcc gcgtccagcg acaacccctg ctcacgccgc
     2941 tcggccagcg cccggtcgag atacaacagc atgatcacgc caaactcgga tgacacgccg
     3001 gccagcgcaa tgaagcctac ggccacagcc accgaaaaat catagccgag ccagtacagg
     3061 aaccacaccc cgcccaccag ggcgaacggt agcgtggaca ggatcagcaa cgcctcagtc
     3121 aggttgccga acacaccgta gagcagcagc agaatgatca gcagcgtaac cggcaccacc
     3181 agcctgagcc gggcttcggc atgctgcagg taagcgtact gtcccgtcca ttcgagactg
     3241 attcccggcg ccagccgcac ctgcgccctg accacgcgct gcaggttgga caccacctcg
     3301 gcgagcggac gcccctgcac atcgatatag acccaggtcg agggacgggc attctcgctt
     3361 ttcagcatcg acggcccggc ggcataagca atgttggcca tggcccccag cggcaccgac
     3421 acccccgctg gcgtcaccac gggcaacgtg cgtaacgctg ccgtcgaatc gcgccaccag
     3481 cgcgggtagc gcacgctgac cggatagcgc gcccgaccgt caatcacttc gccgacatct
     3541 gcaccaccga tcccgtatgt cacggtcgac tgcacatcgg caaggctcaa cccatagcgc
     3601 gccgcctgct ggcgatcgat attcacatcg atgtaacggc cgccagtcag gcggtcggca
     3661 aacgccgaac gcacgccggg caccgtggca gccacctgcg ccacctgccg ggcaatgcgg
     3721 tccatctcga ccggatcgga gccggatacc ctgacaccca ccggactctt gataccggta
     3781 gcctgcatgt cgatgcgatt gcgaatcggc ggcacccaca cattggccag cccgggcaca
     3841 cgcaccaccc ggtccaatgt ctgcaccagc gcggccgggg tcatcccggg ccgccactgg
     3901 ctgcgaggct tgaattcgat cgtggtttcg aacatctcca tcggcgcagg gtcggttgcg
     3961 gtatcggcgc ggccggcttt gccaaacaca tgcgccactt ccggcaccgt cttgatcaac
     4021 cggtcggtct gctgcagcaa ctcggctgcc ttggcggccg acaacccggg caatgccgaa
     4081 ggcatataca gcaaatcgcc ctcatcgagc ggcggcatga attcgctgcc gatccgggac
     4141 agtggccacg ccgtcaccag caccagcagc actgccagca acagcacgct gcgcgggtgc
     4201 gccagcgctg catcaagcag cggacggtac actgccacca gccagcgatt gaccggattg
     4261 ccatattcgg ccggaatcct gccgcggatc cagtaaccca tcaacaccgg caccagcgtc
     4321 accgacaagc cggccgcacc cgccatggcc caggtcttgg tataggctaa cggcgcgaac
     4381 aaccgtcctt cctgcgcctg cagggaaaag atcggtacaa acgacagggt gatgaccagc
     4441 aaactgaaaa acagcgccgg cccgacttcc tgcgctgcct cgccgatgac acgccagcgc
     4501 tcttcgccct gcagcacccg gtccggatgc tgttcatgcc actgctcgat cttcttgtgc
     4561 gcgttttcga tcatcaccac ggcggcatcg accatggcac caatggcaat ggcaatcccg
     4621 cccaacgaca tgatattggc attgatcccc tggatgcgca tcatcgaaaa cgcgaacagt
     4681 atccccaacg gcaatgaaac gatagccacc atggccgaac gcaaatgcca gagaaacagc
     4741 ccgcacacca gcgccaccac caccagctcc tcgatcagtt tgtgccgcag ggtgccgatg
     4801 gatcggtcga tcagcgcgct gcgatcgtac accggcacca cctgcacccc gggcggcaga
     4861 ccatgcgcca gcgcagccaa ccgggctttt accgccgcga tggtagcgcg tgcattggcg
     4921 ccgctacgca ggatcaccac cccgccgacc acctccccct ggccgtccag ttcggcgatg
     4981 ccgcgccgcg cctgtgggcc gatacggaca tccgccacat cccccagcag caccggcacc
     5041 ccgtttgcgc cggtgcccag cggcaccctg cggaagtcat ccacggtctt cagataccct
     5101 ctcgcgcgca ccatgtattc cgcctcggcg agctcgatca ccgatccgcc agtttcctga
     5161 ttggcttcac ggatcgctgc gatcacccgg gactgcggaa tacggtaggc cgccagccgc
     5221 tcggggtcga gcaccacctg gtattgccgc accatgccgc ccagagtggc cacttctgcc
     5281 acgccaggca cacttttgag ctcgaaacgc agaaaccagt cctgcagact gcgcagctgt
     5341 cccagatcgt gccgaccact gcgatcgacc agcgcgtatt cgtacaccca gtccactccc
     5401 gtcgcatccg gccccagcgc gggatgtatt cctgccggaa ggtatgactg cgcctgatcg
     5461 aggtattcaa gtacccgcga acgcgcccag taagggtcgg taccgtcgcg gaacagcacg
     5521 tacaggaacg aatcaccgaa cgaagaatag ccgcgcacgg ttttcgcctc cggcaccgac
     5581 agcatggttg tggccagcgg ccaggtcacc tgatcttcga ccacctgggg gggctggccc
     5641 ggatcggatg tacgcaggat cacctgcaca tcggacaggt ctggcaacgc atcgaccggt
     5701 aaggtcaggg ccgaccaact gccccacaat gcaaacagca ccgtcgccaa cagcaccatc
     5761 acgcggttgc gtatcgacca acgaatgatt gccgcaatca tggctgcgcc cctcccgccg
     5821 gcatcgccat acccggtggc atggcgcggg tggtgacagc atgcgtcgcc gg
//
LOCUS       DAIANL010000163         6238 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_1837574, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000163 DAIANL010000000
VERSION     DAIANL010000163.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837574"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1292 a         2080 c         1767 g         1099 t
ORIGIN      
        1 atgaacaagg gcactgcaga gcaggaacag aacggcgtca cgataccgag cgaggcagcc
       61 atgacgttgc ccaccccgag cctgcgcccc gacagcaggg cgcgggtacg ttccggcgaa
      121 acaaaggtat gcacgatccc catcacgaac acgatgccga caagcaacag cagcaccttg
      181 ggcgtgtcat aaaaaaagaa cagcagcgca ctgcccagcc gactgtccgg cgtcaccggc
      241 agccagctga cgactgctgc ggaaaatggc tccaacccct gatagagggc aaaccagacg
      301 cagatggcca gcaggaaaaa gaaccccggg tggctcgaag gccagcgttt cagtcgaaca
      361 gacaaggcat gcatgtaaac tccgaatgcg ccataagggc gctgtcacac gagaagacga
      421 cgccagaaca aaaaagatgc actgctcacc ccggttgcgc gcgcggaaca aaacagcaca
      481 cccgcccgtc cgtatccagc gtcacttggc aggcggtcat caagtggagg tgcaatcttt
      541 tccgtgcacg ctgcacgcgc gatttggcag ctggtaatgt caggccggca tggtgggcaa
      601 aatcggcctg gctcatgcct tcaaggtcac acagcgtgat ggcttcccgg tcggtcgcgg
      661 acagttcagc cagtgcgcgc ggcagacatt gcgccagcgt gtcaatcgtt gccgtctcgt
      721 gcgtttcgct caccagatcg tccggcaatt cgaccagggt gcgcgccaca cgtagccggt
      781 cagccagggc attacgtgtg acctcgaaca gccatgcgcg tgcattatcc agctcacaga
      841 acctgcgttc gtgccgcaag gccttgatga aaatttcctg caacagatcc tcggcatcgt
      901 gcacgttgcc gaaccggctg cgcaaccagg ctcgcaactc ggcctcatgc cggccccagg
      961 cgttcatcag gcaactcatg gcgcacctcc gtccaccacc ccgaaccgca gcatctcagc
     1021 gcaatatcgc cccatacact tccatttatt cgacaaaacg gatatattgt acttcatgat
     1081 ccagcccggc gcattcttcg acagcctttc cgacagcatt cgcaggcgca tcctcgccct
     1141 gatccacggg caggatgaac tctgtgtctg cgaactggtg gccgcactcg acttgcccca
     1201 gcccaaaata tcacgccatc ttgccgtgct gcgtgcggca ggcatcctgt cgatacggcg
     1261 cgaaggcacc tgggtctatt accgcctgga tgccggcctg ccgctgtggg cctaccgcat
     1321 cctcgagatt gccagccgtg acgccgccga aaccgaatcg gccaacgatc agagaagact
     1381 ggcggacaca cagacgaaac aggagcaatg cagcgcctga atattcgtta tttcaaatat
     1441 atttcttcgc agacaccgac caccccatga actgcaccgt acaattcgcc acagacaagg
     1501 atcacgtcat gagtcacgaa ctgcatccat ccatcgttgc actggtttcc ctcgcggcaa
     1561 gcattgcctc gaaccacccg gcacagggcc tgtgccagct cgaccggctg aaacaatacg
     1621 gcgtcacccg ggaacacatc gatagcgtga tcgaaattgc ccgtcatatc cgcgacgagg
     1681 ccgcgcagca gctcgatgcc cgccttgacg ccatgcgtga cgaccgtgat acgtccagcg
     1741 cccatccgtt cgatgtggtc ggcgtacccg gcggaggcgg cagctgctgc acgccgaccc
     1801 cgtccgggca atcctgctgc tgaacgtcat tcacggagtc ccccatgccc aatacccaac
     1861 acgatcagtt gcgccagcac gtgcgcgacg cctatggcac gattgcccgc gcgggggcaa
     1921 acgcaggggg ctgcggcccc tcctgctgca cgcccagcga cgccgccgtg gccgaactgc
     1981 tgtcgcgcgg catcggctac agtgcacagg ataccgcggc tgtgccggat ggggccaacc
     2041 tcggcctcgg ttgcggcaat ccgcagatca ttgccgcatt gcaacccggt gaaacggtgc
     2101 tcgacctcgg cagtggcgcc gggttcgact gttttctcgc cgcccgcact gtcggtctgt
     2161 ccggacaggt catcggcgtc gacatgacac cggacatgct tgcccgtgca cgcgccaatg
     2221 ccgtcaaggg cagttacgac aacgtcgatt ttcgtctcgg cgaaatcgag cacctgccgg
     2281 ttgctgacgc cagcatcgat gtcatcattt cgaactgcgt catcaacctc tcgccagaca
     2341 aggctcaggt atttcgcgaa tgcctgcggg tactgaaacc gggcggacgg ctggcagtct
     2401 ccgacatcgt gctcaccgcc gatctgcctg aagccatgaa ggccgaagtc gcgctctaca
     2461 ccagttgcgt gactggcgcg gtcagtatcg acgtgctgac cggcatgctc accacggcag
     2521 gcttcgatgc catccgcatt tccccgaagg acacgagtac ggaatacatc cgccagtggg
     2581 cgccagaccg tggcatcgag cactatattg catcggcaaa catcgaagcc atcaagccgg
     2641 cccggtgacg cggcgacggt ctgggcacgg gccattgccg cactgacaaa aatgaaaaac
     2701 ggcctgcaga tcacaggccg tttgacatgg cgactgtaat cagcgaccgc gcccgccgcc
     2761 gcccatgccg cccccgccca tgccacctgc gccaggcccc atgcccccgc caccagggcc
     2821 cattccggca ccaccgaccg gaggctgatc gggcaggctc acgccacgtt ccttcgcacg
     2881 ttctttcatg cgctcatgat gttcgttacg aatctgttcc cgttccctgg cggtcttggc
     2941 gtcacgcaga cgggcacgat attcgttgcg ctcgtgctgc gtcatcagct gactgccata
     3001 aatctgctgc tgactgcgag tctgtgactg ggtttgcgag cgtgtctgac tctgggtctg
     3061 gacctcattc tgggtccgat cctggatctg cgcctgatcc gctgccactg ccgatgccgt
     3121 catcaccgac agtgcacccg caatcgcagc cgtaagcatt acattgtgaa tattcatgtc
     3181 tgactccttg tcgggttgac cgaagatcat gctcacaacg actccaaccc tgtcagccgt
     3241 tgcgccggat ttcccatccc tcccgccccg aactgcagca cgcggtgcga accggaagca
     3301 cgggatgcct tccagattcc acgaccatca tactactgac aaacttatga ctcaaacctt
     3361 tccatatgct gaattacaac atgttttcag cggtgctccg gcaacacagg cgccatcggg
     3421 gctggtcgca gccggggatc aacgctgtcc gatgcgtgcg ccggaaaaca ggggcggctg
     3481 tccgtgtggc aatgagcgcc agtattgcgg cggcgcctcg atctgtgctc ccatcttcgc
     3541 ggcggcatgc cacggccagc gcgggttgta cagcattgcc cgcgccaggg ccaccagatc
     3601 ggcctgcccg gatgccagga tggtttccgc ctgttctgct tcggtaatca gcccgacggc
     3661 aatgaccgga atatcgacct cgcggcgaat acgtgcggcg agcgcgacct ggtagcccgg
     3721 tccgggcgag atgcgttgcc gcggcgagat gccgccgctg gagacatgaa tgtatgcgca
     3781 tcccaacgct gcgagttcgc gcgccagtac ggcgctgtgt tccacattcc agccaccatc
     3841 gacccagtcg gtggcggaaa tgcgaacgcc gaccggtctg tcggcaggaa acccctgtcg
     3901 tacggcagca aaggcttcca gcaccagccg catacgctgt tccagcgcgc cgccataccc
     3961 gtcgctgcgc cgattggcca acggggaaag aaactggtgc agcaggtagc catgggcggc
     4021 atgcaattcg atcgcatcaa tgccaatgcg gccagcgcgt tcggccgcgg cagtaaacgc
     4081 ttgcagaatg cgtgtgatac cgtcagtatc gagtgcgtcg ggcaagggtt cgccatcggc
     4141 atggggcaac gccgatggtg ccagtgtttt ccagccgccg gctgcgaagt caagcaccgc
     4201 accaccatcc cacggcgtct ggctcgatgc cttgcggccg gcatgagcca gctggatggc
     4261 gatcggggtg gcggcatatt gcctgacggc agcgaccacc ctgcccagcg cagcctcgtt
     4321 cgccccggaa tacagtccga gatctgccgg agtgatccgc ccttccggcg tgaccgccgt
     4381 agcttcgatg acaagcaacc ccgcacccga ttgagccagt tgtccaagat gcatgagatg
     4441 ccagtctgtc gcacagccat cccgggcaga atactgacac atcggcgcca cgacgatacg
     4501 attggtcagg gcgaggttac cgatggaaac cggtgaaaaa aggtgactca tggtgtaggc
     4561 tcccgatgca gcgccgtctt gccggaggcc agcccgccaa tggcagacca gtcgagatcg
     4621 ccgccacccg cagcggccag ctcaagaaac cggtcccgca gtacactggc aaacggcaag
     4681 ggcacgctaa gttcatcagc cgcagccaga acaaggcgca agtccttttg cccgagccgg
     4741 gcagcgaaac cggcgggttc aaaccgctgt tcagccacca tggcgccata attgcggtac
     4801 accggcacgt cgaacagcga ggacaccagc agttcgaggt actgatgccg gtcgacgcct
     4861 cccttttcca ccagcgccat ggcttcgcct agcgattcga tgactgaggc cagcagaaaa
     4921 ttgccgctca gcttgaccag gttggcgctc tgcggcgtgg tggaaatgac gaaagtcttc
     4981 tgtccgatca cgtcgaacaa cggctgcaag cgggtaatgt gctgcgccgc gccccccgcg
     5041 accagaaaca gccgtccagc cgcggcggca tcgggccgac cgaagacagg cgccgcgacg
     5101 tacccttgcc cccgttgcgc atgcgcctcg gccagaaccc gcgaacattc gacactgatg
     5161 gtgctgcacg acacatgaat tgcaccggca cgcagatgct gcaacacccc atccgcgccg
     5221 agcaccacct cctcggctgc ccgatcgtcg gacagcatgg taaacaccac gtccgcagca
     5281 cttgcgtccg ccacgctgtg cgccagcgca gccccctgcg cgaccagcgg ctgcgccttg
     5341 tctgcgtggc ggttgaacac caccagcgca tgacctgcac ccagcaggcg tgccgccatg
     5401 cctgtaccca tcctgcccag cccgacaaat ccgatctgca tcaaacctcc tgacagttgc
     5461 ctggacccgg cgcgccagct gcgcgcccaa ttggcccggt gacagcaatt ggcatgcgtc
     5521 aggcctcttc aggcccaagc acgcgtaaca attcctcgac agtggtcaac ccctgcgcca
     5581 ccttgaccag cccatcctgc acgagcgatg ccgtgccgat cgagcgtgcc atacgatcca
     5641 tgtcgaggat actggcacca ctggcgatca tgtgccgcat ttcacgcgtc atcgccagca
     5701 cttcgtaaat gcccatacga ccgtggtacc cggtcttgcc gcaggcactg cagccgcggc
     5761 cacgccagga ctgcaacggt gtctccttgc gcagcaccga accgaaatat tccaccaggt
     5821 gcggatcggg ctcggccggt tcgcgacaat gggggcagat cttgcgcacc agccgctggg
     5881 caataatccc ctccagtgcg ctggccacga catacggctt gagtcccaga tcgaacaacc
     5941 gtgcaatggt cgcaaccgca gaattggtgt gcagggtgga gtagaccatg tgtccggtga
     6001 gcgcagcatg aaacgcaact tcagcagttt cgaaatcgcg gatctcgccg atgaggatca
     6061 catcggggtc ctgccgcagt accgatcgca gtacggccgg gaaattaagg ccgatttttt
     6121 cccggatcaa cacctggccg gccatgtcga gataatattc gaccggatcc tcgatagtga
     6181 catagttctt ttccagcgtg gcattgtatt gcatcagacc atccagcgtc gtggtctt
//
LOCUS       DAIANL010000164        42263 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Thiobacillus sp. isolate clean6591 k141_2042868, whole
            genome shotgun sequence.
ACCESSION   DAIANL010000164 DAIANL010000000
VERSION     DAIANL010000164.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28414145
            Sequence Read Archive: SRR9320188
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thiobacillus sp. (mine drainage metagenome)
  ORGANISM  Thiobacillus sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
            Thiobacillaceae; Thiobacillus.
REFERENCE   1  (bases 1 to 42263)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42263)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..42263
                     /organism="Thiobacillus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042868"
                     /isolate="clean6591"
                     /isolation_source="sediment"
                     /db_xref="taxon:924"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28635 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         8620 a        13692 c        12133 g         7818 t
ORIGIN      
        1 aatcagcagt caccggttca gaatggtatc tacaccatct gggaaaatct gggcgccacc
       61 gaatacaagg gcttcgaact tgaaggcact gcgcatatcg gcatgggctt caacctgtat
      121 ggcaatttcg gttacgacct gaccaaccag gacagcaccg ggcattccat ccaggatgct
      181 ccgcagaaca ccggcgcgat cggtgtaatc tacgaacagc atgcgttgta ttcttccctg
      241 atgaccaagt acgtcggcac gcgcttcatg agcggcggca atggtccagg cggaaacgac
      301 tacttctcgt cctacacggt caccaacttc acgctcaact atactctgcc cggtgtcact
      361 ggctggtaca agagcgccgt gctgggcctg caagtcgaca acctgtttga caaccagagc
      421 atttatgccc tggccggaca ggatgtgcaa ggcaacgatc tgttctggac cctgccgggt
      481 cgtagcgcgt tcgtcagcat cacgttcaac atgtaattgt cgtgccggaa aaaagccacc
      541 gccgcagcgg tggctttttt taacccagca acttgcccag cgtcgctgcc gtgacggtca
      601 gcagaccaag cgacaggttc acagcgatca ccatgcgcat cttgccgagc accgctgcgc
      661 cagccggcca ttcctgtgcc agcacggcac gcttgagctt gccaaacaac gcaaaataca
      721 gatgagcaaa cagcagcatc atcaggatgc ccaacgcggc catgatcatc acataaaccg
      781 caggcttgcc aagctgggcc atcatgtaca gaccgctgcc caaaatcagc gccaccgcag
      841 tccagaccca gggaaagaaa cggccgaaca ccccggccca caatgtcagc cgctgagccg
      901 gttcgagcat ttttgcggca accgggcgca gtgccatgta ggcaaaaaac attccaccga
      961 cccagatgac agcgctcaga acgtgcaaaa accttgcaat aatcatggtc aactcctgta
     1021 aaaacaatga cggcccgacg cactgcatga gtgcacccgg gccgatgggg acaggctggg
     1081 ggctgcgcgt cacagcgcag ttctccttac cctgtcgtga ttgtaaccgg atataccgac
     1141 gaattcatga cgaccacaac aacagttggc gcaccggcgc gaaactgctg cgatgtaccg
     1201 gcgacggtcc gtgcagctgc agcgcagcca ggtgttccgc cgttgggtag cccttgtgcc
     1261 ggtcgaaacc gtattgcgga taacgttcat gcagcgcaat cagctcgcgg tcgcgtgccg
     1321 tcttggccag gatcgatgcc gccgatattg ccggcacact ggcgtcgcca ccaacaatgg
     1381 cgcgcagcgt ggcaggaatg gacaaccgcg gcagctgcag gccatcgacg aggatctcgt
     1441 ccggctggac gtccagcgca tcgatggcgc ggcgcatcgc cagcaatgac gcttgcagaa
     1501 tattgattct gtcgatttct tccaccgtgg caacccccac ggcccaggcg tgcgcctggc
     1561 gcatgatcac cgtttcgagt tcgtcgcggc gacgggcact gaggcgcttg gagtcatcca
     1621 ggccgtcaat cggtcgacgc gggtgcagga ttactgcagc cgcatacact ggcccggcca
     1681 gcggcccccg tccagcttca tcgaccccgc aaatcagtcg catgacggca ggaacggcag
     1741 cacggcttca gcaatgcgtt gtgcggtatc gcgacgcaga ctgtgatgca tctgcatgaa
     1801 cgtctgctcc tggacggcac gcaatttcgg gtcatcccat agcgcggcaa tggcatcggc
     1861 caggtgctgc ggcgtcgctt cgtcctgcag gatttccggc gccagcatgc ggccggacag
     1921 aatattgggc aggcccaccc agggcagata ggcacgtttg cgcatcagcc acgccgtcaa
     1981 ccgcgccacg cgataagtaa tgaccatcgg ccgcttgagc agtgcggctt cgagcgtagc
     2041 cgtgccggag gccaccagca ccaggtcggc agctgtcatg gcgtagtcgg catgaccgat
     2101 catgatctgc acgggcggca ccggatctgc ggcatgcagc gcagtggaaa acagttcgcg
     2161 cgtggcgcgg ctgaccatcg gcacgatgaa gcgcatgttc ggatagcgct cgtgcagcag
     2221 ccgggccgta tccacgaaca gttgcgcatg atgtttgagt tcactgacgc ggctgcccgg
     2281 caacagtgcc acgatcggtg cctgcgctgg caggttgaga cgttcgcgca ccgctttgcg
     2341 gtcagggtgt aacggcagac tgtcggccag cggatggccg acgtaggtta ccgggatccc
     2401 cgccttgcga taaatctccg cctcgaaagg gaacaccacc agcatgtgtg acaccgctcg
     2461 tgcaatcttg cggatccgcc ccccgcgcca ggcccagatc gatgggctga caaaatgcat
     2521 ggtgggaatg ccacgttttt tcagggcgat ttcgagatcc agattgaaat ccggcgcatc
     2581 gatgccaatg aacagatccg gctgttgtgc gatgatctgc tgtttgagcc ggcgacggat
     2641 acgcagcagt tctggcaggt ggcgcagcac ttcggcatac ccgttgaccg caagcttttc
     2701 catcgagtat agcgtgcgtg ctccggcaaa aatcatcttg ggcccggcaa taccgacaaa
     2761 ttcggcattc gggcaacgct cgcgtaccgc gcggatcagg tggctgccaa gcaggtcgcc
     2821 ggatgcttca ccagcgacga tggcaatgcg tggagggcgg gcattcatcg ttcaacgaat
     2881 gatgccacgc tgggagctgt cgagaaaccg caccagtgca gccacttccg gcaccgtctg
     2941 cgccagcgct tcgagttgct cgcgtgcgtc ggtcacactc cttccctcac ggtacaatat
     3001 tttgtaggcg cgcttgagcc cggccaatgc ctcggcagaa aatccgcgcc gcttcaaccc
     3061 ctcgctgttg atgccatgcg gcgccgcggg ctgcccggcg gcagtcacat agggcggaat
     3121 gtccttgaac accacgctgg agatgccggt catgacgtgc gcgccgatct tgcagaactg
     3181 gtgcaccccg gtaaagccgc ccagagttgc atggtcgccg atttcgacgt gaccggccag
     3241 cgatgcattg ttggcaaagg tggtgtggtt gccgacctgg caatcgtgcg cgatgtgcac
     3301 ataggccatg atccagttgt catggccgat ccgggtcacg ccaccatcct gcaccgttcc
     3361 aagattgaag gtacagaact cgcgaatggt gttgcggtcg ccgatttcga gacgcgttgg
     3421 ctcatcgcgg tatttcttgt cctgtgggac ttcgcccagc gaacagaact ggaaaatccg
     3481 gttgccgctg ccgatgcgtg tgtgtccgtg aatcaccacg tgtggcccga cccgggtatc
     3541 gtcgccgagc tgcacatgct cgccgacgat gctgtaggca ccgatctgaa cccgttcgcc
     3601 cagctgcgcc ccgggatgca caatggcgct aggatgaatc gtactcatgc cattctcagc
     3661 gcagggtggc catcagttcg gcctcggtca cgcgttgccc gttcacttcc gcccaggttg
     3721 aatatttcca tattcccttg atgtggcgca ggatggtggc atgcagtacc agctgatcac
     3781 cgggcatgac cggcttcttg aagcgtgcgc cgtcgatgcc ggcaaagtag accacggcat
     3841 cgctttccgg cggtcgtccg accgtcttga acgacagcag cgcggctgcc tgcgccagcg
     3901 cttccagcac cagcacaccc ggcatgaccg gatgtgccgg aaaatgtccg ctgaagaagg
     3961 gttcattgat ggtcacattt ttcagggcca cgattttttc cccaggcacc agctcaatca
     4021 cccggtccac aagcaggaac gggtaacgat gcgggagata ggccatgatt tcattgattt
     4081 ccattgctaa taccttatcc atccattgtt ctgatgttct gacacatccc caggctggcg
     4141 gggcgacgct cccgctgctg cgtactgatg ccaccggaat tggaaacggt tcctgtcagg
     4201 acgaaccggt gccatccgca gcatctgcat cctcgagctt cttctccagt ttgcgcaacc
     4261 gcttcgccat atcgtcaaga tggcgaagat gcaccgcatt cctgagccat tcgccgtgtt
     4321 cggtaaacgg cagcgcggaa gtataggtgc ctgcgcgcgg caacgatttg gtgatcaacg
     4381 tattggtcga aatgttgacc cggtctgcga tttcgatatg gccgaaaatc atggccgcgc
     4441 cgccaatggt gcaatgtgcg ccgatgtgtg cactgcccgc tatgccggtg caggcagcaa
     4501 tcgccgtgtg ctcgccgata tgcacgttgt gcgcgacctg gatcagattg tccagcttga
     4561 cgccatcgtg aatgatcgta tcatccagcg caccacgatc gaccgtagta ttggcgccga
     4621 tttcgacatc gttgcccaga accaccctgc cgatctgcgg aatcttcagc cagcgaccgt
     4681 gctccctggc cagaccgaac ccgtccgatc cgattacgca accgctgtgg agaatcaccc
     4741 gatcaccgat gacgcaatgg tgataaacgg tgacgttggc gtacagcagt gcctgttcgc
     4801 cgataatgca gtcctcgcca atgacactgc cggctccgat gacgctgcca gcaccgatac
     4861 gggcaccgcg ctcgatcact gcattggccc cgatgcgcac atcctggccc agctgcgctt
     4921 ccggatgcac gagggcaccg ggctggatgc cgggctcgcc ccgcagtggc gggttgaaca
     4981 gtgccgagac gtgggcaaaa taagcataag ggttgtcact ggcgatgctc gggcgttgcg
     5041 ggagctcatc cagattgttg ccgatgatga aggccgacgc atgactggtc gcgagttggc
     5101 tgcgcagttt cggattggag aaaaaactga tctgtccttc gccagcccgg gcaagactcg
     5161 ccacctgggt gatacgcacg ttgccgtccc catgcaacgt gccaccaaaa cgctcgacca
     5221 gttcagccag ggtataagcc gtcatgattc atggttccgt tgtgatttac tgggacaaca
     5281 tgtgaatgat gtggtcggtg atgtcgagtt tcgggttgta gtacacggca tcttccagaa
     5341 tcacatcgta tttttcgctc tcggcatacg actggatcgc cttgcgcgca cgatcctgca
     5401 ccttgatcag ctcgtcgttg cgccgtgaat tcagatcttc gcggaattct cgcagcttgc
     5461 gctggtattc ccggctcagg ttggcgagat cgcgctcctt cgcgctgcgg tccgaaccgg
     5521 tgatggtgtc ggcattgcgg tcaagatccg tctgcatgtc acggatctgg cgggacatct
     5581 tctgcaattc ggcatcacgt ggcgcaaatt ccttttccag ccgtttctgc gcggcaagcg
     5641 caatcggtgc ctcacgaaac acccggtcgg tattgacgaa gccgatacgg gtatcggcct
     5701 gcgccaccgc gggcagaatg gcggtcaacc acatcaggca caacacaaag acaatccggc
     5761 gcatcgagat ctccttggct gacgtcagaa cacctgaccc agtgtaaact ggaacggcgc
     5821 agtcacgtca taaggctgcg gattcagcgc cttggccagg ctgaatttga gcggaccgac
     5881 cggagagatc cagttgaccg caaaaccgac ggaatagcgc atcggctcgg tcgagaacgt
     5941 gtcaccgggt ccgaagatgt caccgccgtc gaggaacgtg ctcaggcgaa ccgtatggtt
     6001 atcagccatg cccggcatgg ggaacatcag ttctgcgctg gccagcaggc tcttgttgcc
     6061 acccatggcg atgtattcac cgtaggtcgg gtcgtaatac ttcgggccga tggtggaaat
     6121 atcgtagcca cgcaccgaat ccacgccacc ggcgtagaag ttcttgaaga acggcaatcc
     6181 ctggccattg taaccaccgc ccatacccac ctgaccgttc agcatgaggg tgaatgtttt
     6241 gctgagcgga tcgaaccact gttgctggta ggaaatcttg tagtaatcga gacttcccgg
     6301 cggcacgccg atttcgccga acacccgttc gagcatgccc tgcgtcgggt agatcaggct
     6361 gtcgcgtgtg tcacgtgccc aaccgagatt gaggcgcacg gtatcgttgt agttaccata
     6421 ctggttgacg aattgctgat actggatcgg actgttggaa aacacgtcga ggtggaattt
     6481 ttcgaacgcc agaccgaggc tcatggtgtc cttttcattg agcggcacgg ccacccggcc
     6541 accgataccg atggtggtgt tcttgtaggg cgacaacgta tacatggccg tggtatcggt
     6601 cacattcttg taaatatcgt aaccggtgct caggccgtcg actgtcgaat acggattggt
     6661 gtacgacagg gagtacacct ggctgaacag accgctgttc acggtaagcg ccagggtgtt
     6721 gccgctgcca aacaggttgt tctgcgagat cgatcctgac aggatcagac cttcctgcga
     6781 agcaaagccc acgccggcca tgatgcttcc ggtgggcaac tcggtcaccg aaacgttgac
     6841 atccacctga tcgttggtgg ccggaacggc gggagtttcg atgttcacgt cgctgaaata
     6901 gccgagtcgc tgcaggcgct cccgcgaacg ctggatcagt gcggcatcgt accagctgcc
     6961 ttcgagctgg cgaaactcgc ggcgcgcaac ctcgtcacgg gtgcgcgtat tgccggtgaa
     7021 gctgatgtgg tgcacataca cccggtgacc cggatcaacc atgaaagtga agccaacggt
     7081 gtgtttgtcc ttgtccacat cgggcacggc gttgacgttg gcgaaggcat agccgtcatt
     7141 gcccagccgg tcgccgattt tcttcgaggc atctgtcacc ttctggcgcg agaaaatgtc
     7201 gcccggctgg atgtcgatga gtttgcgcag ctccggttca ggcaccagca gcaacccgcc
     7261 gagcttgatg tccttcaccg tgtagcgcgg tccctcggtc agattgatgg tgatgtagat
     7321 gtctttcttg tccggactga tcgacacctg tgtcgaatcg atgctgaatt cgagatagcc
     7381 acggttgaga taatacgaac acagcgtctc caggtcggcc gacagtttct cgcgcgaata
     7441 ctggtcattc ttggtgtacc agctcagcca gtccggcgtg ttgagcttga tcgcatcgcg
     7501 caattgtttc tcggtgaaca catggttgcc gatgatgttg atggcgtgga tcttggccac
     7561 ctggccttcc ttgatgtcga aggccagcga cacgcggttg cgcggcaacg gcgtgaccgt
     7621 ggtctggata tccaccgaat agcggccatg gctgtaatac aaccgcttga tttcctgcgt
     7681 ggcgcgatcc atttgcgcct tgtcgaaaat ttgcccttcg accagtccgg cctgcttgag
     7741 gccgtccttg atctgatcgg cggtgaactc tttgctgccg ctgatggtga tcttgttgat
     7801 ggccggacgc tcaatgacac gcaccaccag cacatcaccc tgacggtaca ggctgacgtc
     7861 cttgaaaaac ccggtggcat acagtgcatg catggcctgg tcggccttgt cagcgtcgag
     7921 cacatcccca accttgaccg gcagataggt gaaaacggtc ccgggttcgg tacgctggat
     7981 gccctctacc cgaatgtcct tgaccacaaa gctatccgcg gcatgggctg aaatcgccca
     8041 tgccgccgaa accagtgctg cagcgatttt tctttgcatg ttcacgaacc caacaaacgg
     8101 gtaatatcat tgtagaaagc gaaaaacatc agagtcagca gcgcagccag acccagccgc
     8161 tgccctaatt cgactacccg ttgcggaaca ggctcgcccc tgagaaattc agcgatatga
     8221 tacatcaaat gccccccatc caataatgga atgggcaaca aattcagcac gcccaggcca
     8281 atgctgatca gcgcgaggaa attcgcataa gcaacaccgc ccaggcgtgc gctctggcca
     8341 gcataatccg caatggtcag cgggccggac aggtcatgcc agaacgcgcg ccccagcacc
     8401 aggtcacgca gcatgtccag attgagtaca atgccctgcc aggtacgcac cagcgccccc
     8461 tgcaatgcgg gcagtaacgg gtagcgtacg gtcgtcagca gttgcgccgc aagttccgga
     8521 tcgatctcgg gcccggcgcc tatcttgccg acagcccggc ccgcctcgcg ttcggccgcc
     8581 ggggtcagcg taaccgtcag cagtctgccc tggcgatcaa tggcaaactc caccggccgg
     8641 cccggacggg cgcgcaccag ttcgaccagt tgttgccagt cgctcacagc gataccatcc
     8701 accttgcgga tgcgatcacc ggtacgcagc ccaccccgta ccgcgacacc atctgcaatc
     8761 agggtgccaa tgactggtgc gatgctcggt tcgaacacct tcagcccaag ctgactggtc
     8821 agcgcatgtt gcgcatcatc cagtttaagc cccgaaaccg gcaacacttc ggaactgccg
     8881 tcggcgagcc gcacccgaac cggctccgtg tcgccggcat ggcgcagcag gatccagttc
     8941 aggtcatccc atccacgcac cggttcgccc gccagacgca cgatctcatc atgcgcgtgc
     9001 aggccggcac gggcggcggc actgccggcg ggcggttcgt ccagcagcgg acgcaccatc
     9061 tggacgccgt gcaacagtac cccccagtac accagcaacg ccagcagcag gttggcaagc
     9121 gggccggcgg caacgatcgc catgcgcacc ccgaccggtt tgcaattgaa ggacgcgctc
     9181 cgctcgtgtt ccggcacggg cgcctcgtct tcgtcgagca tcttgacata gccgcccagc
     9241 ggaatcgctg acagggtcca ttcggtgcca cgcggcgaac tgcgccagct ccagagtaca
     9301 ggtcccagac cgaacgagaa gcgcagtaca cgtaccccgg tcaaccgggc gaccgcgtag
     9361 tgtcccagct catgaaatgt caccagtatc cccagcgcga ctgcaaacgc cagcagtgtg
     9421 gtcaacatca tcatgatacg cagtgctgcc ggatccagcc ctgcgccatt tcgcgcgccc
     9481 gcgcatcggc atcgagcaat tcggccagcg aagcgggtac tgcgccatcg ctgcattcga
     9541 gcgcgtgacg cacggttgcc gcaatctggt cgtaacggat cacaccatcg agaaatgcat
     9601 cgacagccac ttcgttggcc gcattaagca ccgccggcat cgtaccgccc tgctccagtg
     9661 cgtcatacgc cagttgcaag cagggaaaac gggcaatatc cgggacttca aaggtcaggt
     9721 tgcggccgaa cagatcgagt ggcgtcacac cggattcaat gcgttgagga aaaccgagtg
     9781 cataggcgat cggggtgcgc atgtccgggt tgccgagctg ggccagcaca gatccgtcga
     9841 ggtaatccac catcgaatga atcacgctct gtggatggat gaccacctcg atctgtcgcg
     9901 aagccgcacg gaacagccag tgcgcttcga tgacctccag tcccttgttc atcatggtgg
     9961 ccgaatcgac cgagatcttg cgtcccatca cccagttagg atgcgcgcag gcctcgtcgg
    10021 gagtgacgaa cggcagttca gccagcggcc gggtacgaaa cgggccgcca gatgccgtca
    10081 gcaggatgcg ccgcacacca tggtccgcga gggtcccgtg catggggtac ggcagactct
    10141 ggaaaatcgc attgtgctca ctgtcgatcg gcagcagttg cgcgccacct tgcgccaccg
    10201 cctccatgaa caggctaccg gccatcacca gggtttcctt gttggccagc aatatccgtt
    10261 tgccggcgtg cgccgccgcc atggccggcc gcagtccggc cgcgccgacg atggccgcca
    10321 tcacgctgtc ggcttcgggc agcgccgcga cctgctccag cgcctcgatg ccgtgcagca
    10381 cttcggtcgt acaacccgtc tgctgcagca tgacccgcaa gcgttgcgcg ctatcaccat
    10441 ccatcaccac agcataacgt ggtcgatgct ggctgcactg ctgtgccagg cggtcgatct
    10501 ggctgctgcc ggtcagtgcc agcacttcga aacgctcggg atgccgggcc accacgtcaa
    10561 gcgtgttgac accaatggta cccgtcgcgc cgaggacgac cagcttgtgc aacgtgctca
    10621 tgaccagctc cgcaatacgt cggaatgcag caggatcagc gctgctaccg gcaaggtgga
    10681 tgtcagtgca tcgacacgat cgagtatgcc gccgtggccc ggcaacagat tgccactgtc
    10741 cttgacatct gccagacgtt ttatccagga ttcaaacaga tcgccaataa tgctcaggta
    10801 cagcatgagg attgcaagca gcatgatcgg cgtcatccat acgcgcggct cgaactggtg
    10861 gctggcaaac agccacagga tcatgccact gtacaacaac acgccgacca gcgcaccggc
    10921 cactccttcc caggtcttgc ccgggctgat caatggtgcc agtttgtggc ggccgaatgc
    10981 cttgccggag aaataggcag cgctgtcagc cacccacacc agccccatca acagcaacag
    11041 ccaccacgga ccacgcgccc gcaattcgat cagcgccgac cacagcggcg ccagtaccag
    11101 aaacccgagc ggcaggtgca gccatagcga acggatcttc catcccgctg ccagccacac
    11161 gggaacgatc agcagccaca gcacgagcga agccgcgtac agccagactg aaccacctcc
    11221 atgccaggcc gccaccgtca gcagcgaccc gagcgcgacg tatgccacca gtggcgccgc
    11281 ggacaagcca gcaagccggc cccattccac catgctcgcc accagtggca gcaacatcac
    11341 cacccaccat gctggctgcg gcagccagaa caggcagccg atcaggccga taaacagcag
    11401 tacggccgtc aggatccttt tctgcagcat gttgtcaatc cttcatgccg ttgccacggc
    11461 caactgtcgc cacgtcaacc acccacctgt tcgctggtac gcccatagcg acgctcacgt
    11521 gaacggaaag aggtcatggc ctcatcgagc gctccggcgt cgaaatcagg ccacagcgtg
    11581 ggtgtaaaat aaagctcggc ataggccagc tgccacagca ggaaattgct gatgcgctgc
    11641 tcgccgccgg tacggatgaa cagatcgggt tccggagcat aggaagtgct caggtacggc
    11701 gccagcatct gttcggtgat tggctgaccc gccagatccg gctggtgggc cagcaatcgg
    11761 gtcaccgctt gcagcacgtc ccagcgtcca ccatagttga cggcaatggt cagtgtcagc
    11821 ccctggttgg ccgcggtcag gtgctggcca tggtgaatca ggtgcttgag cttctcgtca
    11881 aagggagtca ggtcaccgat gaacttgagc ctcacattgc gttcatgcaa cgaatggatc
    11941 tcatgttcga gcgccagatg gaacagttcc atcagcagcc cgacctcctc actcgggcgc
    12001 cgccagttct ctgaactgaa ggcaaacagg gtaaggaatt cgacgccacg gttggcgcaa
    12061 gtgcgaacga tttcgcgcac catgtccacg ccacgcttgt gtcccgccac acgtggcaga
    12121 aaacgtttct tggcccagcg accattgcca tccatgataa tggctatgtg gcgtggaatc
    12181 gctccggttt ctggaatgac ctgggtcgaa ctctggtagc tgaacacggg ttacaccgcc
    12241 atcaggtcag cttccttggc cgcaagcgcc ttgtcgactt cggcaatgta acggtcggtc
    12301 agcttctgga tgtcttcctg agcacggcgt tcgtcatcgg tgctgatctg cttgtccttg
    12361 agcaatttct tcagctgctc gttggcatct cggcgcacgt tgcggactgc aaccttggcg
    12421 ttctcgcctt cgccacgcac caccttgatc aggtcgcgac ggcgctcttc ggtcagcggc
    12481 ggcattggca cgcgaatgac gctgccctgg ctggacgggt tcagccccag atccgcatcg
    12541 cggatcgctt tttcgatcgc ctgggtgagc ttggcttccc acggttgcac agcaatggtt
    12601 cgcgcgtcga ccagattgat gttggccacc tgactgatgg ccgtgggatt accgtaatag
    12661 tcgaccatca catgatcaag caggccggta tgcgcccgtc cggtacgcac ctttcccaga
    12721 tcggtcttga atgcctccag gcattttccc atgcgcccat cggcggattt tctgacctcg
    12781 tcgagcattg ttgtctcctt tcagcaatga acgatggtac cttcatccat gccactgaca
    12841 aaacgcgcca gtgccccgga attgaaaata cccagcactg ccaggggcat tttctggtcg
    12901 cggcacaatg tgaaggccgt cgcatccatg acttgcaggt tcctgtgaat ggcttcgtcg
    12961 aaggtcagcg tccggtagcg tgtagccgcc gggttgcgta caggatcctc actgtagacg
    13021 ccatcgacct tggttgcctt gatgacgata tccgcgttca tttccatgcc gcgcagtgct
    13081 gcggcagtgt ctgtcgtgaa aaatgggttg ccggtgccgg cgccgaagat caccaccttg
    13141 ttttcctcaa ggtagcgcat ggccttgccg cgaatgtagg gttcggccac ctgctcgatg
    13201 gtcagtgccg actgcacccg gctcggcaca ccggcaacac ggaaggcatc ctgcagtgcc
    13261 aacgcattca tcaccgtggc aagcatgccc atgtaatcgg cggttgcccg atccataccc
    13321 gctgcagccg gagccacacc acgaaaaata ttgccgccac caatcacgat ggccacctgg
    13381 acccccatgt caatcacgct tttgacttcc tgcacgatac tgacgatggt gtcgcgattg
    13441 atgccatagc tgtcgctgcc catgagggct tcgccagaga gcttgagcag gatgcgcctg
    13501 taaaccggtg acggcatgat ggtcacacct tggccgcagc ggccacttca gcagcgtaat
    13561 cgaccgtttt cttctcaatg ccttcaccga cctggaacag ggtgaatcca ttgacgcgcg
    13621 cctgcttgcc ggccagcaac ttctccaccg tctggtccgg atccttgacg aacggctgac
    13681 ccagcagggt gacttccgcc agatacttgg agatgcgacc ttcgaccata cgcgcgacga
    13741 tatcggcggg cttgcccgat tcggcggcct gcgcactgta aatttcgcgt tctttctcga
    13801 tcagttcggg ggacacctga tctttcgata cacagaccgg cttgctggca gcaatgtgca
    13861 tcgccaggtc tttccccagc atctcgtcgc cgtggatatc gatcatcacg ccgatgcgac
    13921 tgccatgaag gtagctgacc aggcgggcat cggtacggta acaggctgca cgccggatgc
    13981 tcaggttttc gccaagcttc atgatcagcg actggcgcac ggcttcgacc ttgccttcgg
    14041 ccaggtcgag tgcggcgatc tgttgcagat ccgttgtctg ttgtcttgcg acctgttcgg
    14101 ccacggcttt ggcgaaggca atgaagtctt cgttgcgcgc cacgaaatcg gtctcgcagt
    14161 tgatttcgac cagcgcgccc agtttgccgt cggcgctgat ccacgagccg atgacccctt
    14221 cggctgccac acggccggcc gccttgctgg ccttggcgcc gctcttgatg cgcagcaagt
    14281 cttcggcggc ggacatgtcg ccgtcggtct ccgtcagtgc cttcttgcac tccatcatgc
    14341 caagaccggt gcgctcgcgc agatccttga ccatgctcgc ggtaatttca gccatcccct
    14401 actcctgttt acgtttggtt ccaattcatg ggcagattgc cgcgctgcct cgtgcaccgt
    14461 ccggacacaa ggccgccgca gttgcacgca acggcgcagc ccggccggtt ccggcatcat
    14521 gcgatctgca cgaccatctt tcagcaacct gtgaaaaaag gggccagcgc cccttttcat
    14581 caagcattca accgcgcatt atcccagatt cccgtgcagg acgcgatacg cccggcacga
    14641 tgaaagtttg cgcggccggg ccgccagccc gtcatcactc ggcagcggga gtggcaaccg
    14701 gttcgtccag ctcgacgaat tcctcaccct cacccagcat ctcggagatc gattcgctgc
    14761 gtccttcgag aacagcatcg gccatgccgc gcgcatacag gcgaatggca cgactcgaat
    14821 cgtcgttgcc agggatgacg tagttcacac ccagcgggct gttattggta tcgacaacgc
    14881 caatcaccgg aatgcccagc gtgttggcct caacgatcgc gcctttctga tagcccacgt
    14941 cgatcacgaa aatggcatcg ggcagcacgt tcatgtcttt gattcccccc aggctgcggg
    15001 tcagcttttc cagctcgcgc tgcagcaaca gggcttcttt cttgggcagg cgcgcaccat
    15061 cggcaagcag cgcctcaagt tccttgaggc gcttgatgga ctggcgcacg gtcttgaaat
    15121 tggtgagcat gccgcccagc cagcggttgt caacataagg catgcctgcg cgctcggctt
    15181 cctcgcgcac gatctcccgc gccgaacgct tggtgccgac aaacaggatg gtgcccttgt
    15241 ttgcagccag cttgcgggcg aacttgatcg cctcctggaa catcggcagg cttttttcca
    15301 gattgacgat gtggatcttg ttgcggtgac cgaaaatgaa cggagccatt ttcggattcc
    15361 agaaacgggt ctggtggcca aaatggacac cagcttccag catttgacgc atggtcacgg
    15421 tcatgacaat ttccctcagg gttaagcctc cagtcgccgt gacgtgcctg ccccgagcct
    15481 tcctggccga agcccagcgc gccaacattc ccggtcaggc cgggaacacc cctacgacga
    15541 acgactgtgc ggattttgat gccggccggc gcaaccgcgc gccatttgac ttgccgatca
    15601 catcaagccc gcaatcctag catgaaccgc cctgtacggc aagccagccc gggctatgcg
    15661 tcgtcctgac cgtccacgcc cgtcaggatc cattctgcca ggccagccag cacgatctcg
    15721 ctgtccccgg gcgtggacag atcggtcagg tcgatctggg ggaaacgctc atgcagacgc
    15781 gccagcatca caggatagtc gcgcagaaaa tgggcacccg gaccgagaaa cagtggcacg
    15841 atgcagatgc ggtcgcatcc gcagccggcc agaacactta ctgcgtcttc cagcccgggc
    15901 gacatgaatt cgagccaggc caggccaatg cgccagtcgg gccggtgccg ggctacccga
    15961 tcgcgcagac ggagaaacgg agcatcccac ccgggcacgc gcgaaccgtg gccaaacagg
    16021 atcaggccgg aagtcatgca cccaccgctg ccgtccgcca tatcacagcc cgccgcccga
    16081 tcatgcggcg cccgatgcca gttcaccggc atcgcgggag ggaccgcgtc cttctccgcc
    16141 tcgctggtca atgtacaggc tcgaaccgga aatctgcttc gcagccttct tgacttctgc
    16201 cgcaacggcc gaaatctcgt gaatctcgtg aaactggccc gggtgcaccg gcaccacgcc
    16261 gatggacagc gtcggccagg aatattccat gcggttgccg cgccggtctt cggcaacgta
    16321 acccagtgga tcaagcccct gctcgcgcac ataatcgctc accgcgcggt cgtagctggc
    16381 gaggatgttg cggcagatca gggcccagtc gtcgctttgc agcaggatga agaagtcgtc
    16441 gccaccgacg tggcctacga aatcgatctc gggctgggta aattcgacca ggatgttggc
    16501 tgtcagcttg atcaggtcat caccacggcg gtagccgaac acgtcattga acggcttgaa
    16561 gttgtccaga tcgacatagg cggcctgaaa gcgctgcccg ctgtcgagca gcgcctgcat
    16621 ctgctcgctg atcggcacgt taccgggcag caaggtgagc ggattggcat accgcgcttc
    16681 ctggatctgc tggcgggtga tttcctgcat cagcgagaaa ttggagccga cgccgacata
    16741 cttgccatca ctgaccagga taaagccgtc accgaagcgc ttgcgcccct gcaacagcac
    16801 cagctggctt agcgcgacga ccggcatgtt gatgtccacc accagaggct ggtcatccat
    16861 gaacaccgtg cacgacttgc ggctgtgcag atcgcggata tagccgtggg agaacttgtc
    16921 taccatggtc cggttgagca ggccgaccgg tcggctctga tcgatcaccg gcagcacatt
    16981 gagttcggga ttggcgagca ggatctgcag cgcggcttca ctcgacgtct gcggtgtcac
    17041 cgtcggcgcc tcggtcaaca gcacgtgcgc gcgctggccg cgctgatggc cggtttttcc
    17101 ccgtccggcc gaggcattgg ccgcataaaa catctgactc cattccggac gcatgtcgag
    17161 cttgaagtca ttggccggtt ccgcggctgg cacgccaatc aggttgccct gtccgtaaca
    17221 cacccccatg ctctttaacg tgacaagttg ctgcggcaac tcgatgccga cagcgatcag
    17281 cctggcgttg ctcttttcag ccatctgctt gatcgaccgc acgaactgat gtctcgtcac
    17341 atcggcgtcg atctggctga ccagcggccg gcccagcttg atgtaatccg gccgcgtatc
    17401 cagccacaga cgcagacagc ccgcactttc accaacatcg ttgagcgcat agcggaagcc
    17461 cttgtcgcgc aacgccgcca gattctcgac aatcgagaca tgatctgcct gcaccggatt
    17521 ctcgctgatt tccagcacca cgcgctcacg caggccacct tgcttgccca gcaatttttt
    17581 cactgcctga tcggcgagtg cacctgtcat cagcgcgtac aggctcaggt tgagaaacag
    17641 ttttcccggc atgccgcgcg cgacaaacgt ctcgacctga gcctgcaggc tcagcacctc
    17701 cagccgcaac cgcagatcaa gctgtccggc cagcggaaac agcacctcgg gcaaatgcag
    17761 ctcggactcc tgcggaccgc ggatcagtcc ttcaaaaccg atcacctgcc cctgctgaag
    17821 gtccagtatg ggctggaaca cagggtacag acggcgcttg tcgagaattt catgcaagga
    17881 tttgcgtcga gacataactt tcccgcttca ggttgcggag tcgatggctg cgggatatcg
    17941 ctccgcccac catccaatga ctaccttagc gccatctaat ttcaatctga tgacaaaacg
    18001 ctgtccattc atccgctgtg cagccagcag caacccatcc gtccattcca tcgacggctg
    18061 ggcgcaccgg catggagcac acatgcggca atatgcacgg caccggacat ccttcacgca
    18121 aaacaaaaac ccacccggtt gattttaaat aaaatttttc tggcccacta tttgcttcaa
    18181 caccagcggg gcgcaggttc ccggaacacg cgccctatct tcatcctcca tgcggtattc
    18241 agggcatctt cggatgccct cttttttgcc cgacctgatg ctgcattcac gccggcagca
    18301 gcgactgctt cacccgctcc agtcgtgcac gtacctcgtt ggccagaccg gcgatctccg
    18361 ccttgtccac cagcgtaagc acgctcaccg ggtccatgaa cgccaccaca gtcttgccgt
    18421 cagtcgtggc gcgcacgacg acattgcagg gcaacaacag accgatatcg ggttcggccg
    18481 tcagcgcctg atgcgccagc ggcggattgc acgcccccag aatgcgataa ggcagcccct
    18541 cgacgcccag cttttccttc agcgtgccgc tcacatcgat atcggcaagc acgccgaaac
    18601 cttccttgcg cagagccgcc accacatcgg tgatcgtctg gtcgaaaggc gtatccagtt
    18661 ctactgaaaa tccatacatg gtcattctcc tgaaaaactg cggctcccgc gcgcgattcg
    18721 ctcctggcgg cgggctgctg tacaatcgcg catccgattt tacccgattc cacccctacc
    18781 gtgatccgcc tgcaccaact cgcgctggcg cgcgcctcga aactgctgtt cagtgacgcc
    18841 agcctcgacc tgttccccgg acagaagatc agtctgactg gcgccaatgg cagcggtaaa
    18901 tccagcctgt tcgccttgct gcgcggccag atcgaagccg aagccggacg gctcgagatg
    18961 ccgccgcact ggcagatcgc ccacgccgcc caggacacgc cggcactcgc caccagcgcg
    19021 ctcgatttcg tgctcgatgg cgataccgga ttccgtacac tggagcgcga catcctccgc
    19081 gctgaacagg ccggcgacgg cgcacacctg ggtgaactgc atgcccggtt cgatacgatc
    19141 ggcggatacg ccgcgcgcgc ccgtgccagc gcactgctgc acggcctcgg cttcgccgat
    19201 gcgcgcatgg gcgatgccgt gactgatttt tccggcggct ggcgcgtacg cctcaatctc
    19261 gctcaggcac tgatgtgccg gtccgacctg ttgctgctcg acgaaccgac caaccacctc
    19321 gatctcgaag ccatcctctg gctggaggac tggctggccc gttacccagg caccctgctg
    19381 ctcatctcgc acgatcgaca gttcctcgat gccaccacgc aacacacgct gcacatcgaa
    19441 cacaaccagc tcacactgta tgcaggcaac tacagcgcat ttgaacgcca gcgggccgaa
    19501 cggctggcag cgcagcaggc gcagcacgag gctcagcagc gccgtcgcgc gcacctgcag
    19561 gactatatcg accgcttccg tgccaaggcc accaaagccc gtcaggcgca aagccgcatc
    19621 aaggccctgg cacgcatgga atcgaccaca gcggcgctgg ccgattcgcc gttcgaattc
    19681 agcttcctcg cgccagaacg ctcgcccagc ccgctattga cactgaaaca ggcgagcgca
    19741 ggttatggtt cgaccgtaca actgcgtggg ctggactgca tcatccagag cggcgaccgg
    19801 attggagtgc tcggcagcaa cggcgccgga aaatcaacct tcatcaaact gctggccggg
    19861 gtattgcccg tgctttgcgg ggaacgcgtt gccggccacg gcctgaaaat cggttatttc
    19921 gcccagcatc agctggaaca gctcgacccc gcggcatcgc cgctcgaaca tctgcaacac
    19981 ctgtcgccag aaatggctga acagacactg cgcgactatc tggccggatt cgattttcgc
    20041 ggcgaccacg cactcacccc gtgccagtca ttttcgggcg gagaaaagtc gcgcctggcg
    20101 ctggcgctga tgatctggca gaaacccaac ctgctgctgc tcgacgagcc caccaatcac
    20161 ctcgatctgg ccatgcgcca tgccattgcg ctcgccttgc aggaatatgc cggcgccatg
    20221 gtattgatct cgcacgaccg cgaactgctc gaaactgcct gcgatcgctt cgtgcgtatc
    20281 gcccacggca cgttgcagcc cttcgacggc gaccttgacg attaccgtgc cgtactgcgc
    20341 agcgaagcgg ccacacccgc acacgcggca cagctcgacc agcgtcgcca gcgcaaacag
    20401 gacatggtcg aacgccagca acggctacaa cgccagcgca agctggcacg cgaatgcgaa
    20461 caactggaac agcagctcga tgcgctgggc gtcgaccgca aaatatgcga ggcagcgctg
    20521 gcggatacgc cgttgtatca gcctgaaaac agggacgcgc tcgcacagac gctagaacgc
    20581 aaccgcgccc tgggtgaaca gaccgaaaca ctggaaaccc gctggctgag cctccaggaa
    20641 cagcttgccg cgctcgaaca ggaacttcag gacggcgacg gcggtactgg caccggctga
    20701 ggctgcctgc cgaccacgcg cgacagcgtt ccatacaacg acggactgaa cagtcgcgac
    20761 accagtgacg agaacaggct gacggcaatc aggctgatga ccatttcatg accatcgatc
    20821 atctccatga cgatgatgca cgaggtgatc ggtgcctggg tgacggccgc cagaaacccg
    20881 gtcatgcaca acgccaggat acttgccgac gacaccatgc cgcccaatac cggcagcagg
    20941 tcctgaccga tgcccacgcc aatcgacagt gccggcgcga aaattccgcc aggcacaccg
    21001 ctaaagtacg acgccagcgt tgcaaggtac ttgcccgggg caaatgtcca cggttcgccg
    21061 gcactgccgg acagcacggc acggctcgca tcgtagccgg aacccagcac ggaaccatgc
    21121 gtggcataac cgagcacccc gacgacaagt ccgcacacgc cggcaaacca caccggatgc
    21181 tgttcacgcc actgaccgat cgagcccggc cacggatgcg ctgaccacac cagcaaccgg
    21241 ctgaaccagc cgcccagcat gccgcagacc agactgatga gcagcacagg cagcatcacc
    21301 tggctgtcaa cgcgtccaac cgccagatgc ccgaaataca ggtaattgcc ctgcaacgaa
    21361 atcgacacca tgcccgagag tacgatcgac gtcagaatca cgccgttggt gctctgctca
    21421 aagctgcgcg acaattcctc gatggcaaac accacgccgg ccagtggtgt attgaatgca
    21481 gcagcaatac cggctgcgcc gcccgccagc acgagatgcc gggggtgaat cacatagcct
    21541 ttcggcagca ggtggcgaaa cgcgtgcatc acgcttgcgg ccacctgcac cgaggggcct
    21601 tcgcggccca tggagaatcc gcaaccgact gccatggcgc cgaacccgac cttggcaacc
    21661 gctatgcgca tcgagaccag cttcgacacc gattccccgc gttgcatcga ttcaattgca
    21721 gccatggtct gcggaatgcc gctgcccgcc gcagccgggg accagcgacg caccgcataa
    21781 acgatcatca tgcccgccag cggcggcagc aacagggaca ggacgcctgt gtggccccca
    21841 aacttgccgg tttcattcca gcaccactgc gtcagcatgg tgaaccccac cacgaccagg
    21901 ctggcacaga tcgcagccat ccagatcacc agacgttcgc gccaggcgcg caattcgacc
    21961 agccggtatt cacgcaccga ttcaagcggg gttttcatac acaacatttc gggacaataa
    22021 accccgattg tcgcactaac cggcttgtct tttccagtgc ctgcatcatg tttgcaatca
    22081 ccgtggcgcg ctcatcggcc agcgcactgg caatcagcgg tgcgctcagg ctcaggcgca
    22141 aggccagcaa cggttcatcg gcgtcgccgc cgcacaccac cggttgaccg agcagaacag
    22201 gcacacgggc tattccgtcc ggcaacccgg ccgttgcggg caacgtttgc tgtatgcatc
    22261 ggtaaatgct ccgtaccgtg gccgcagcca gcggctgccc gtttgcaggg tcacacaaca
    22321 gaaaggcaaa cagggtgggc gcatcgccgt ccgcagcatg cagacggtcg ggcacatcac
    22381 catcaagcgg acgaaacacc gcatcgtcgt gcaatcggcg catcaccgca tcgccaaaac
    22441 ggctgacgca agcgcgaacc gcggtttgcg gcaacgcgga aaaccgttcc atctcgaaca
    22501 gcgccgcctg ccagcgcaac agcagtggca gactgtcttc atccatcacc tgcgggtccg
    22561 cgtccacagc atggtccacc ggccacagcc aggcaccgga aaaactcggg ccgccgtaga
    22621 atttcgatcc cgtcagcacg atgccaaaac cgcattgcag gtaatcacgc aacactgccg
    22681 gatccatgcg catctgacac gcatcgatca gcacatccac ccgaccagga tattgctcgc
    22741 gcaatgacag cagggtggca agccccggag cgaccaatcc gcttttggac acatccgcac
    22801 ggatcaccag cacctgttgc ccgcgcagca ccgcttcagc agcatgctgc atcacttcgg
    22861 catccacttc cgtgcgtgcg cgggcaccac catcggcgtc gcgcagccga acagcgtgca
    22921 cctggcatga ctgtcccgcc gcagccgcca gcatcgcgcc ggcaacgccg ctgccggtct
    22981 cggcgtgcgc tggcatgatg atgtacagcg gccggtgacg ccaggtcagc aaccagcgca
    23041 cgatcctggc atgcgcatct gttcctgaag ccgccagttc cagtcgcccc gcatgcggcg
    23101 ccacgccgag ccagcgcagc agacgcgcgc gcacctgtgc atgcagtgcc tgctgcgcat
    23161 cggccagcgc ccgcgcatca gcgcagtgct gcaggcgtgc atactccgcc cgcacgttgt
    23221 gccaggctgg ctcactgatg accgaagccg tggaagaagc gaaggccacc agttggggat
    23281 cgggaaacgg ccggcagcca taacggttga cgcccgtggc ggcatcgacg gcaatccgct
    23341 cgtcgccgcc ctgcgtcagc agcagcgaca atggcggcca gtcctgctca gtcccggaca
    23401 ccatcggtaa tcatgcgttg aaacgcggca aacacgccgt gcatgtgcgg tgccttgtat
    23461 gggaacagtt ccggcggatc catgtcatgc acgatcatgt tgctgtcgat ctcgaaaacc
    23521 agcaacctgc cgtcgggact ttcaccacag tcgataccca gatagtcgag gccactgcgg
    23581 gccgcgattt ccgccagcgc ggctgcatgg cgcgcggcaa attcttcgcg gaaatgctgc
    23641 atgctgtgtg cttcctcagc ccgattggcc gggttgtcga gcatgccggc gctcagatag
    23701 tgcaccatcc agtgatgcga gatggccaga tgacacaggt gcggcactcc gcgcatgagc
    23761 acgatgcggt attttcgata ccagccgtct gcgttgcggt attcgacgaa gcgcgacaga
    23821 tagaactggt tgcccgcctg ccgttcaaga tactcagcca gtccggcatg ttcggtcagc
    23881 ttttccagcc cctgaccggc atgcgaatcg acgggacgca cgatgaccgg aaacccgccc
    23941 tccccggtcc attgcgccgg tgactgctga ccggcagcga tcagttccag gtcggcacgc
    24001 gtcacgcgca cagtacgcgg caattcgata ccggaaatgc caccaagcag cacgctcacc
    24061 tgatcgcgcg acaggcgccg aatcgcttcg gggcgattga ataccggtgc ggtccagtgc
    24121 tgcagccaca gtgcaagctt gtccagcagc aagcggtttt cactcgattc ggccacggcg
    24181 acgaacactg catcatgcgg cggaagttcc ggcagtgcac cgtcttcggc acagacatag
    24241 acgagatcaa acgcaatctg gctgtgctcc agcagaaact cgaccggcga attggccatc
    24301 atgtcacccg gtccgacaat caccgccagg cgcaggctga ctggttcgcg gtgtgccggg
    24361 aaggagaata cgcgccggat gttcagtgcc tcgcgctgca tcgccaggcc aaaggcagga
    24421 ttgccgcgca actggaaaat aatggacaga tccagcatgg cttcagctgc gtccgaccgc
    24481 tggcgtgcgt attcgatcaa tctggagccc agtggagcca gatcgatgcc tttcatcgac
    24541 atgcgcagca gatcaggcaa gccgatccat ggcacggccg tctgctgtgg agtggttacg
    24601 gtttcagtca cgcgttgctc ctgcaagtcg ggcaccctgc tgcaatacgg catcgaccag
    24661 gcggtcgatg tcttcggcgc gggtacggtg gttgacgatt gccgcacgca acacaagacg
    24721 gccttcgaga acggtggtcg acggtgcggc cagaccagaa tgctgcaccg ccaccgcgat
    24781 cgctgcattg accgcatcgg cagcctcggc agcatcgcaa cggtaggtga aacacacgat
    24841 attgagtgcg accggcgccc gcagttccag catcggttct gccgttatgc gttctgccag
    24901 ataacgcgcc agcgcacatg tctgcgccat gatctgcccc agacggtcgg taccgtatac
    24961 ctgtaacgtg aaccaggtct tcagcgcgcg gaaaccgcgc gacaggtcag gaccgaaatc
    25021 gcacggccag ggcgaaccgc cggccagacc atccgcttca cggcgcaagt aagccgcagg
    25081 cgaagcaaaa gtgccgaggt gcaactcacc gtcgcgcacc agcacgaatc cggcgtcgta
    25141 aggcacctgt ccccatttat ggaaatcgaa ggcaatcgag tccgcgcgcg tgatgccggc
    25201 aaacaggggc gcaagctgtg cattcagcat accgagcgca cccagcgcgc catcgacatg
    25261 aagccatacc ccgtgcgcct gcgcaatgtc ggccagatcg tcgagcgggt cgatggcgcc
    25321 gcaatcgacg gtaccggcat tgccaaccag tagaaacggt gtccacccgg cttgccggtc
    25381 agcctcgatc tggcggcgca gtgcatcgac gtcgatgcgc tgcgccgcat ccacgtcgat
    25441 acggcacagg cagtcgctgc ccaacccggc cagatccagt gcctgcgcca cgctgccgtg
    25501 ggcggctgtc gaggcgtagg cacgcagcct gagcgggtgc tgcgccaacc cctgacgacg
    25561 caccgccacc ccgagatagc gcacgcgcgc cacccagacc gccatcaggt tggcctgaga
    25621 cgtgccggtg acgaacacgc cgcttgcctc agcgggaaaa tcgaacagct cgcgcatcca
    25681 gcgcacgatc tgccattcca cctgtaccgg aatctggtta cgcccaccca gattggcatt
    25741 catgcccgca gcaagcattt cggccagcat ccctgccagc gtgccaccgc catgcaccca
    25801 acccatgaaa cccggatgaa tgttgcctac gccatacggg aggatatcgt tcatgaaacg
    25861 tgcatggatg ctgtcgaggc tcccgggctg gcgcggcagc ggctcggcga aacgcgcgcg
    25921 cactccgtcg ggcgcagcct gccatacggc atgctggcgc aggtgttcaa ggtgatcgag
    25981 catgtcgtcg agcatgccgt gcgcctgatg acgcagcgtc gcccagtcgg cgggatccag
    26041 gctgtcaggc gcaggatcct gaaaaagttg gctcacgcgt attcctgtca aagatcagac
    26101 cggcctccca ccggcccgcg acggcccgtt cgcgctcacg gagcgagcgc gtcctacccc
    26161 gtcagtccgt aacgcttgag tttgcgatac agcgttcgtt cgctgatgcc gagtatatca
    26221 gcaacattgc gccggtgtcc gttgtaggtc tgcaataatt cggcgatgta ttcggcttcc
    26281 agttgcgcga tcgggatttc aggacccggc aggcgaacgc cctcgggcag cgcttcctgc
    26341 tgctgctcga caggcaccgg tttggcgaag gcggccggat ggctgaacag gatatgttct
    26401 gcctcgatga gatgcgaacg gctgatcgcc accgccttga ccagcagatt gcgcaattcg
    26461 cgcacattgc ccggaaagtc ataggccatc agccgcagca cggccgcttc gctcagcttg
    26521 cagcgtggcg cacccgcctc cgccatgcga cgcaggatca cgtccgccag cgccgggatg
    26581 tcgctgcgac gctcgcgcag gctgggcaga cgcaaatcga tgccggcaat gcggtaatac
    26641 aggtcgagcc ggtactggcc ggcctcggcc agcctgagca gatcgcgatt ggtcgcagcg
    26701 atgatgcgta cattggagtg caaggtttcc gtaccgccga cacggcggaa ttcgccactg
    26761 tcaaggatgc gcagcagctt ggcctgcatt gccatcggaa tctcgcccac ttcgtcgaga
    26821 aacagcgtgc cgccatcagc cagttcgaac agtccctgtt tgcggccgac gcagccggta
    26881 aacgcaccgc gttcatggcc gaacagttcg tcttcgaaca tggtttccgg gatggctgca
    26941 caattgatta ccaggaatgg ccggtcgcgg cgcatcgaac gcttgtgcag ataatgcgcc
    27001 gccagctcct tgcccacacc actctcgccc atcagcagca ccgccgcttc ggattcagcc
    27061 gctcgtgaca acagttcgac acaatggaga aatgccgggg aacttccgac catgcgcatg
    27121 tcgtcgcaac tgaactcctc gggatcgacc acatgcatga ccacttcccc cagatacttg
    27181 acgtccgcgc cgccacgaat cgggtagccc ttgatacgca catactcggg ccgacgttgc
    27241 ggatcaaagt gtgtatgcat gacctcgtgc acctgcccgt tgcggaatac cgcctggtgc
    27301 ggacagtcct cgccattctg gtgacagggc accggcgagt gatgcgatac ctcatggcaa
    27361 cgcttgccaa gaatctcgtg ccgcttgtag ccataatatt cgcaataggc ctgattggcc
    27421 gcgacaatgc ggtattgcca gtcgatcagt acgaaggggt gggcatgcgc ttcgatcagc
    27481 gaagcaactt ctggcgccag cctgtcattc atgcggcctc ctgacaaaaa gtcatgacgg
    27541 atgacaatca tggtacggca agtttgccag aatgtcatga cacttcagtc tcctcatgac
    27601 catttcaaat gcaacacatt gaaataaaat gattcattca tctggcacag gacgtgctaa
    27661 cactacagcg cacttcctca taccaatgaa aggattccgc atggcacaca tcgtgatact
    27721 cggcgcaggc attggcggtc tgcccatggc atacgagatg aaagaactcg cccgcaagga
    27781 agacaccgtc actgtcattt ccaactcgcc ctatttccag ttcaccccgt ccaacccgtg
    27841 ggcggcggtg agctggcgca agcgtgacga gatcaccttc ccgcttgcac cacctctgga
    27901 aaacaagggc atccgcttca tcgcccacgg tgccaggcgt gtgcatcccg aacagaaccg
    27961 catcgagctg gaaaacggtg aatccatcga ctacgactat ctggtgatcg ccaccggccc
    28021 caagctggca ttcgacgaag tgcccggact cggcccgcaa ggccacaccc attcggtctg
    28081 tcacatcgac catgccgaac agtcgagcca gcaatgggaa aatttctgcg cagcgcccgg
    28141 accgatcgtg gtcggcgccg tacagggggc atcgtgctat gggccggcct acgaatatgc
    28201 catgatcatg gacaccgacc tgcgccggcg caagatccgc gaccgcgtgc cgatgacctt
    28261 cgtgaccgcc gaaccctatg tcggccattt gggtctgggc ggtgtgggcg actcgcgcgg
    28321 catgctggag tcgatcatgc gcgaccgcca catcaagtgg atctgcaatg ccaaggtcac
    28381 caaagtcgaa cccggcaaga tgttcgtcac cgaacacaat gatgatggca gcgtgaaaaa
    28441 agagcacgaa ctgccgttcc actactcgat gatgctgccg gccttcaagg gtatcgatgc
    28501 ggttttcggt attgaaggac tgaccaaccc gcgcggcttc atcctggtcg atccctacca
    28561 gcgcaatccg aaattcaaga acatctactc ggtcggcgta tgcatcgcca ttccgccggt
    28621 agaggccacc ccggtgccaa ccggtgcacc caaaaccggt tacatgatcg aaagcatggt
    28681 caccgcagca gcgcgcaata tccgtctcgc gctggacggc gcggaaccga ccgaaaaggc
    28741 cacctggaat gccatctgtc tcgcggattt cggcgacacc ggcgcagcgt tcgtcgctct
    28801 gccgcaaatt ccgccgcgca acaccaactg gttcaaggaa ggcaaatggg tgcacctggc
    28861 caaggtgggg tttgaaaaat acttcatccg caagatgaaa aaaggcaaca ccgaaccgat
    28921 gtacgaaaaa ttcgtactca agaccatggg tatcaccaag ctcaagtagt ctcctctgac
    28981 cgggttgttt gtccggtttt ctccaaacgt ctgctggcaa cgcgccagcc ggcagacgtt
    29041 ctttttcttt actctcccgc ctggactggc tgcagaacac agcccggacg gatcgccagc
    29101 cagcgcctca cacccacgcc acttgccgtt tcggcatcca gctccatgcc gttgaaaaag
    29161 gtagcgagga cacccggatg caaggcgcga gaaacgcaac gacgagacat atcaaatcga
    29221 caggcgtggg agtgagtatc gcagcaacgc cgcggacggg tgccgcagta ctttttcaac
    29281 ggcctgtcta atgtgctcct aagcaacgca caggtaacat ccgggcattg cctgccggag
    29341 tccacgcatg aaatttcgtt tctggtctgc catcctggtc gtattggccg gtcttccctg
    29401 cacgcacagt tcagcgtccg gactgccact gcagatggtt gccgacattt ccttgcccgg
    29461 tcatgccagc cggctggatt atgcctcgat cgatgccatg cgccaccgcc tgttcatcgc
    29521 ccatctcggc gacagcaatg tcatcgtgtt cggactcgat gcccgcaagc cgctggccat
    29581 cgttcccgac attggccatg tgcacggcgt actggcagtt gcagcacttg gtcgggtgta
    29641 tgccagcgcc acatcgagca atgaaatcgt cgacatcgat gcgtccacgc ttgccatacg
    29701 cgcccgcagc gcaggcgggt tctaccctga cggcatggca tgggtgcctg gacacaatga
    29761 actctacgtg tccgacgagc atggtcgcag cgaaaccgta atcgatacgc gcaccgacca
    29821 gcgcgttgcc acactggcgc tggacagcac cgttggcaat tccgcattcg acagtgcgtc
    29881 gggacacgta ttcgtcaacg cgcaaagcgc caaccagctg gtggaaatcg atcccgcccg
    29941 ccacgccatc atggcccgtt atgacctggc gggcgcggac agtccacatg ggctgctcat
    30001 cgacaccgcc gaccggctcg cgctgatcgc ctgcgaaaac aaccatcgcc tgctggtgtt
    30061 cgatctggac agtcatcggg tgaccgcatc gtttccggtc ggcccggaac cggacgtact
    30121 cgcattcgat ccacaattac atacgctgta tgtggctggc gaacagggag tggtttcggt
    30181 gttttccatc acggcaaccg gggtgacaaa aaccggcgag ggttttcttg ccgacaacgc
    30241 ccatgtggtc gtgatcgatc cgacctccca tctggtgtac ttcccgctca gggatgtcgg
    30301 cggaacgccg atgctgcgca tcatgcgccc gcgtcccttg cccgcagacc gtcactagac
    30361 cggcagcccc agcgccttgt tgagcgccac ccaactggat gccgcctggg tacgcgccac
    30421 cagcatggca atgcgcgcat ccgtcagcgc cagctggctg gtcagccggt cctggcgatc
    30481 gatttcacct gcggcaaacg ctgcctgagc atgcgcatcc gcccgctgcg ccgcatccag
    30541 ggcgtcctgt tcacgttgca gcgtatcgag cgcatggcgg taatcagaca gcgcgcgctc
    30601 ggcgtcgtcg agcgccgcca gcacgacaga cacatacgcc tgggcagccg aacgctcgcg
    30661 cgcctgcgcc gcatgaactt gcgcttcgat gcggccgcca tcgaaaatct gccagctcac
    30721 caacggcatg atggtgccgg tctgactggg catggtcagc cagttgcccg gttcgatgga
    30781 ttgaaacccg gctccggcac tgatcaccag ttgcggaaac cattgcgcct tcgccacacc
    30841 gatttctgcg gtggcggcgg caagctggcg ttctgcgaca cggacatcgg ggcgacggcg
    30901 cagcacgtcg gctcgctcac cgaccggaat tccagccaga cgcggaaaga cacccggact
    30961 gtcgagcagg tacaggccgc tctccggtgg ccgtccggta agtatgccaa tcgacaatgc
    31021 cagcgcccgc agcgacgccc gcatggccgg caactgggct gcggcagcat cgcactgcat
    31081 caccgcctgg tcgagcgcgc tttgcggcac atctcgcgcc gtcacgcggc gctttacggt
    31141 atcgacattg tcctgcagca gagacacaac ctgctgcctg gcctgccatt cacgctgttc
    31201 accgcgcaac atcagatagt tacgcaccag ttccgccacc aggctcatgc gcacatcctg
    31261 cgcgctggca atcgccgcct gatgctgcgc ccgcgcggct tcgatcgtac gccggttggc
    31321 tccgaacaga tccgtatccc aactggcgtc aaactcgaca tcgcggatgg tttcgtcgcg
    31381 gggaatgaat ggaatctgtc gcaacggcag cgggccgttc tcgctctgtc gcagcgcatt
    31441 cacgctggcc gcgatgccgg cctgcggcag cagcagcccc atcgctgcgt ctctggccgc
    31501 acgcgcctgc acgatgcggg cccggctggc ctgcaggtcg gggttatgtt gtagcgccaa
    31561 cccgaccagc cggtttaact cggcgtcgtg cagcgattgc caccaggact gggcggccgg
    31621 caccgccgtg gatgcgggct gcgaccagcc ctgaccaatg tcggtatgcg gcgcgtggta
    31681 atccggcccc acggtacagg cagtcaggcc agtcagggcc agccccccca cccagtgcca
    31741 tcgtttcagg ttcatgatgt tcctcattgc aatcgactgc gcacaaatcc ggtcgccgcc
    31801 gccagcgtga ccagtgcaat cagcagcatc ggccagtcgt tggcccacag caccgcggcc
    31861 ggcaggtcct tgagaaaagt gccctgcaat acgaccaggt aatagcgcag cggtattccc
    31921 atggacagcc attgcaggaa caccggcatg ttttccgtgg gcgtggcgaa tccggacatg
    31981 agcacgctgg gcacgacaaa gctgaacgtc cccagaatgg cctgctgctg ggtggaacat
    32041 acggccgaaa tcatcagacc gatgccgacc accgaaaaaa tgtacagcac caggctggcg
    32101 aacagtcccg gcaacgagcc ggtaaacggc acatgaaaca gaccggcggc cactgccacc
    32161 atcagcaggc caagcaccac cccgaccacc agcgccggcg ccatcttggc gatgatgatc
    32221 tgggccggag tacagggcgc gaccagcaac tggtcgaacg tgccgagctc gcgctcgcgc
    32281 gcgatcgaca gcgccgtcag cagcatggtg atgaacgtca ccaggatgcc gcccacgccc
    32341 ggcaccacga accactggta gaccaggttg ggattgaacc agtggcgcac tggcaccagc
    32401 gtatcgatcg aggaaacaga acggttttcg gcatcgaaac gcgccagcat gctgttcagg
    32461 taaccgacca gaatctggcc ggagttggcg cgccggccgt caatgatcaa ttgcagatgc
    32521 gcctgcctgc ccgctgccac atcgcgcgat gccgtctcgg gaatcaccag cgccgccatg
    32581 acacggtgct gctcgatcat ctggtcgagc gcctgccggc tgtgcaccgg gtagagccgg
    32641 tagacgaaac tcgcgtgcgc cacccgctcc acccagttgc gcgaccagat gccggtatcc
    32701 cggttgtata cggccagact ggcatggctc agctccagcg tggcggcaaa cgaaaatacc
    32761 agcagctggg cgatcggtgg cacgatcagc accagccgtg ttttcgggtc gcgcagcacg
    32821 ctgagcagtt ccttgatcac cagcgcgcgc aatgcggcgg aaaacagggc catcggctat
    32881 gcctccagcg acttgcgcag ccgcaaccgc gcaatgagga aaaacatcgc gccgatgccc
    32941 agcatcgccg ccagattggt ccacagcagc ggccacacat ctccgaccag gaacagcgtc
    33001 tgcaggctgg acacgaaata gcgcgctggc acgatatgcg tcagcagccg caccggccac
    33061 ggcatcgaat cgatctcgaa cagaaagccc gacagcatga atgccggcag atagcccgac
    33121 agcagcgcga tctgcgcggc aaaaaactgg ttcttgctca caaccgagat caacagcccc
    33181 tgtcccagcg cagggatcag gaacacgctc gaggtcagca gcagcgccgc ccatgaacca
    33241 cgcaacggca cgtcgaacac gtggatggac agtgccgtgg cgatcagcat cgccagcagg
    33301 ccaagcacaa aatacggcaa cagcttgcca gccaggatct ccgccaccga caccggcgtg
    33361 gccatcagcg cttccatcgt gccgcgttcc cattcgcgcg ccaccagcat ggccgtcagc
    33421 aacgtaccga taatggtcat gacgatcgcc agtgcgcccg gcacgagaaa acgccggctt
    33481 tccacctcgg cgttgaacca gaaacgcggc tcgatgaccg gcgccacggc aggcacctcg
    33541 ctgcgatcgc tcagccattg cccgacgacc ccttgcgcgt aggcattgac gaaactggct
    33601 gtattgggct gtgcaccatc ggttacgacc tgcaccaacc cgtccgcacc gcccacagcc
    33661 agccggcggt cgaagtcctg cgggatcacc acgaaaccac gcagttcacc cgccaccagc
    33721 cgcgtggcag cctcgcgacg gatacgcagg ggcaacacac gaaaatagcg ggttcccgag
    33781 aacgccgccg ccagcgactg ggcctgcgtg ctgtcgcttt gcaataccac cgcgatgcgc
    33841 acctggcgga tatcgagtga cacggcataa gcaaacagaa acagcaggat caccggcagc
    33901 acgaatgcga tcaggatggt cgacgggtcg cgcacgatct gcagggtttc cttgcgcatc
    33961 aacgcggcca gccgccacag gtcgaagccg gttggccgaa cttcgggagc ggtcatgctg
    34021 cctccggcat cgcatcgaca cgctcgatca gacggatgaa tgcctgttcc atggatggat
    34081 cgggcatggc gacatcggcc acctgacgct tgagtccgtc tggcgaatcg agggcaatca
    34141 cctttgactg atacatcagc gccacccgat cgcaatactc ggcttcttcc atgaagtgcg
    34201 tcgtgaccag gatggtgaca ccgcggtgca ccagcccgtt gatgtgggtc cagaattcgc
    34261 gccgcgtcag cggatcgacg cccgatgtcg gttcgtcgag aaacagtacc gctggacggt
    34321 gcatcagcgc acaggccagc gcaagccgct gacggatacc cagcggcagg ttctccggcg
    34381 aactggacag atatttctgc aactggaaaa tttccaccat ttcatcgcgg cgctcacgct
    34441 tgtgcacacc ttcgaggcca tacacgcccg ccgaaaattc aaggttctgc cgcaccgaca
    34501 gcaatccata gagcgaaaat ttctgtgcca tatagccaag ctggcgcttg gcttcgctac
    34561 gcgcactgcg caaatcgatc cccatcacac gcgcctcgcc cgatgtcggc ttgagcaggc
    34621 cacacagcat gcggaaggtg gtggatttcc ccgcgccgtt cggcccgagc aagccaaaga
    34681 tctccccctg gcggatctcg aaattcaccc ggtcggcagc cacgaaactg ccaaaacggc
    34741 gtgtcagttc atggcatgcc accgtcgctc cggcatgtac tggtaccggc gcgaaatgct
    34801 gcgccagcac cgaagtgccg cccgggccac cgccaagcag atcgacgaaa gcatcttcaa
    34861 aacgtggcgc caccggctcc atgcggctat ccgcctcaag cgccagctgc cttgcaggcg
    34921 cagcatcttc cccggcgcgc agcaccaccc gcaacgactc tccctggatc acgccatcgc
    34981 agacctgagg caggtccagc gcctgcgcca accactggcg ccggtcgccc tgcggcgccg
    35041 gtatcttgaa actgcgtccg gtcagacgcc gggtcagttc ctgcggcgct cctgaaaatt
    35101 ccagttgacc gccattgaac agcagcacag tctcgcagcg ctcggcctcg tccagatacg
    35161 ccgtcgacca cactacggcc aacccttgcc cggtcaattc attgaccatg cgccacaatt
    35221 cctggcgact gaccggatcg accccgacgc cgggttcgtc cagcaacaac acctttggcc
    35281 gcgccatgag cgcacaggcc agaccaagtt tctgcttcat gccgccagac aactgtccgg
    35341 ccagacgatc gacaaacggt tccaggcggg tgaatgccaa caggtcgcca aatacgcccg
    35401 attgctcacc cgcgggcagc gcacgcaggc gggcatacag gcgaaggttt tccattacgc
    35461 tcaggtcttc atacaaacca aaacgttgtg gcatatatcc agaaacaaca tgaatatcat
    35521 cggcctcgcg taccgtgtcg tatcccgcga ccgaaatgta accggcatcc ggcgtcagca
    35581 atccggccag caagcgcagc aaggtggttt ttccagcacc atcaggcccc acgaccccgg
    35641 taagttgacc ataggcaatg gtggcattca cctcacgcag cgcctcgaag ttgccaaacc
    35701 gtttgctgac cgcgtctacc acgatagccg tagcaggcga agctgcgggc cggaccgatg
    35761 cgttcacgtc gggttccgtc cgtgacccat cacacggatg gtcaccggca ttccctgtcg
    35821 cagggcctgg tccggatggt tgatggtgat gcggatgcga tagaccagat cggtgcgcag
    35881 atcggtcgtc tccacggtct tgggcgtaaa ttcggcccgc ggtgacacga aaccaatcgt
    35941 gccgccatac tgtgtcgggg acgaatcgct gctcaccttt accacagtgc ctggcgccac
    36001 ctggccgagg tcggactcgc ttacataggc gcgcacgaac accggctggg tctggctcag
    36061 gatgtagacc ggcgcggtcg ggctgaccat gctgccagct tcacgtatgc gcgaaaagac
    36121 gatcgcgtcc gctggggcct ccagacgggt gtcgttcaac gccgtctgtg ccgcctcacg
    36181 gttggcgcgt gcggcggcca gttgcgcccg ggcggcagcg atgtcttcgt tgcgcgaacc
    36241 ggccagcatc aggtccagtt gctgacgcgc ggcggcgagg ctcgcctggg cagcatcgct
    36301 ggcggcgcgt gcggcgtcaa ccgtacgctg gctggcagtg ccgcccggga gcagcgactg
    36361 ttgtcgcacc aggtcggccg aggcattggc ggccaccgcc tcggcctgat gcacggcttc
    36421 gcgtgcttcg gcaatctgct gtggccggtt gccgtgaagc aacaggtcga gcctggcctg
    36481 cgccgcctgc acgttggcct gggcggctgc cagcgcttca cgaaacggta cgtcgtcgag
    36541 cgtcgccagc atctgcccgg catgcacgtg ggcaccctca tcgacctgca ccgttgccaa
    36601 ccggccactc tgacggaatg ccaggtcaat ctcgcggata tccacatccc catacaggat
    36661 caacggacca gccgccgcat gatccaggcg ataatactgg tacccccccg ccgacgccac
    36721 caacagcagc aacgctgcaa accattttgg ttgtatgggt tttttcatgt gcgaattttc
    36781 agtaattccg gaacggtctc ttgttatgaa cggctagcgc aagaccagct cgaaatcccc
    36841 acgctcggcc accgcatcag cgaggatctc cagcagcatc cagtactcct cgcccaacgc
    36901 atcgcgggtg gttccgggac gttcccacac cagccgcagc ggcccttccg cctcgatcag
    36961 cgcgtcccac aaaccatcca tgtcggcttc gaagccacgc gggaatgcaa atgcctgcgc
    37021 aatctcggta aaaaaccgct gcagcgtgga gacatcctgc aaatggcagg ttttcatgcc
    37081 ggaatccatc tcagaggact tctccggcat ggtcggccag acgcgaacgt tccccacgcg
    37141 ccagagtgat gtgaccgcca tgctcccatc ccttgaagcg gtcgacgacg taggtcaggc
    37201 cggaactgcc ttcggtcagg tacggagtgt cgatctggga gatattgccc agacagacca
    37261 ccttggtgcc aggcccggcc cgggtaatca gtgttttcat ctgtttcggc gtcaggttct
    37321 gcgcttcgtc gatgatcagg aatttattga gaaaggtgcg tccacgcatg aaattgagcg
    37381 acttgatctt gatgcgcgta cggatcaggt cctgggtggc ggcgcggccc cagtcgccgg
    37441 ctgcatcgtc ggtcttgttg agcacgtcga ggttgtcttc gagcgcgccc atccacgggc
    37501 tcatcttttc ttcctcggtg ccgggcagga aaccgatgtc ctcacccacc ggcacggtca
    37561 cccgggtcat gatgatttcc gagtagcgct tgtgttcaag cgtctgcgtc agccccgccg
    37621 ccagcgtcag gagcgtcttt ccggtaccgg cctgccccag cagcgtgacg aaatcgatct
    37681 ccgggtccat cagcacattg agtgcaaaat tctgctcgcg gttgcgtgcg gtgatgcccc
    37741 agacattgtt tttctggtgg ctgaaatcgc gaatggtctg cagttcggcg ctgcgatcgt
    37801 ccactgcgcg tacgatggca tgaaacggtg attcaccttc caggtagacg aactcgttga
    37861 ccagcaactg gctcaccagc gggccgcgaa tccggtacca ggtgtgcccc tcgacctgcc
    37921 aggactccat gccgttatga tgcgcctccc agaaatccgc tgacagttcg cgcatgcccg
    37981 aatacagcag gtcggtatct tcgagcacct tgtcgttgaa gtaatcttcg gcaggcaatc
    38041 cgagcgcgcg cgccttgatg cgcatgttga tgtctttaga caccaggatc accgcacgtc
    38101 ccggctgctc ctgacgcaga ctcaacacca cgccgagaat ctggttgtca accttgaccg
    38161 aaggcaaggt ctcgggcagg ttgccctgca tcgcacgggt ctgcaagtac agtcggccgg
    38221 tggccgactt gcggctcttg ctgtacagtc cgatgccctc ctcgatggag gcttcggagc
    38281 cggaaacgat ctcgtcgagg aaacggctgg cctgacgcgc attgcgcgcc acttcagtca
    38341 tgcccttctt gtgctggtcg agttcttcca gtgtcgccat tggcaggaac acgtcgtgtt
    38401 cttcgaaacg aaacagactg gtcggatcgt gcatcaacac attggtatcc agcacgaaca
    38461 gtttggtggc cactgcaggc ttgctcgaca tctattgttt ctccgccagt gttttgagtt
    38521 gatccagcac ttcctgtgcg tggccggccg aacgcacgcc acgccactcc tgcaccagcg
    38581 tgcccgacgg atcataaagg aaggtgctgc gctggatacc caccacatcc ttgccataca
    38641 tcttcttgct gtggatcaca tcgaacaggg cacataccgt accctccgca tccgacagca
    38701 attcgaacgg cagccccagc ttggccttga agcgctcgtg cgaagccaga ctgtcgcgtg
    38761 aaacgccgac tatacgcgca cccaggcgct cgaatccgtc atgcaggtcg cgaaaatcgg
    38821 aaccttcaac cgtacatccc ggtgtgtcgt ccttgggata aaaatacacc accagccatt
    38881 tgccacgcgc atcgtgccag tgaaatgtct gtccacccgt ggaaggtagg gaaaattcct
    38941 gcattgccga ttcctttacc gggccacgcc cgatttcaac agtatgacca ccgcgccact
    39001 gccaccatca gcctgacgcg cctgacaaca ggcgagcacc tcgtcgcact ggacgagcca
    39061 gtgtgcaacc agtccccgca ataccggccg gcgatgggaa gatcccagcc ccttgccatg
    39121 aatgatacgc acacaacgtt ggccctgacg tacactgcgc gcaaggaaac cagccacggc
    39181 caggcgcgcc tcgtcgcgca ccatgccatg cagatcgagt tcggcctgaa tggcccagtg
    39241 accacggcgc agacgacgca gggtgtcgcg ctgcatgccg ctgcgcagaa acgccggcgc
    39301 atcgccatcg ggtacctcgg catcatcgcc gaagaggcca tcgcttagcc ggtcatgcac
    39361 ggtcggcagc gtttctgtga tccggcgccg cagtggccgc aacactggcg actgcgatgc
    39421 aatgcggccg tcggcggcca gcggctgcac atcgcttacg accgcgcgaa acagtgcgct
    39481 gtcgcaggga tcgcagttac gggactgacg gctggtcatg gcatcggatc aggtcagcga
    39541 ttcaaggtag cggtcggcgt ccagcgccgc catgcaaccg ctacccgcgc tggtgatggc
    39601 ctggcggtaa gcgtgatcct gcacatcacc cgcggcgaac accccggcga tgctggttgc
    39661 ggtcgcattc ccgccctgcc ccgcgcgggt gaccagatag ccaccgctca tttcgagctg
    39721 accgacaaac aggtcggtat tgggcttgtg gccaatggcg acgaacacac cggtgactgc
    39781 cacatcccgc agagcgccgg tttcggtcga gcgcaggcgc atgccggtca cccctgaatc
    39841 gtcgccgagc acttcctgca gttcggaatg caacgccagc gtgatcttgc cctcattgac
    39901 cttttccatc aaccggtcga tcatgatacg ctctgacttg aatctgtcgc gtcgatgcac
    39961 cagcgtcacg tggctggcga tgttggccag gtacagcgct tcttcgacag cggtattgcc
    40021 gccaccgatg acggccaccg actggttgcg gtagaaaaat ccatcgcagg tggcacagcc
    40081 cgacacacca cggccgagga acttctgctc cgattccagc ccgagataca tggccgaggc
    40141 accggtagcg atgatcagcg catcgcaggt gtaggttccc tgatcgccga tcagggtgaa
    40201 cggcttgctg gtcagctgcg cggtgtggat gtgatcgaac acgatctcgg tgttgaaacg
    40261 gcgggcatgg cgttcaaagc gttccatcag ttccggtcct tgcaccccgt cgacgtcagc
    40321 gggccagtta tccacttcgg tggtcgtcat cagctgaccg ccctgagcca taccggtcac
    40381 cagcaggggc tgaagattag cgcgggctgc gtagattgca gcggtgtaac ctgccggtcc
    40441 cgatccgaga atcagcaggc gtgcgtgtct ggttgtgctc atgattttcg cgtgactcca
    40501 tgaattctgt atatgaccca ggaattctag caggattggc gcaacggtta acctgcttca
    40561 cgttataatt ttgccaatat ccgacggatg ccctcgcatg tttcgtttcg cttcccgcaa
    40621 tcgcacgacc tcccgtcacg catccgaccc ggctggtgct ctgccacgcg aactgttctg
    40681 gctgctgcct ggctgtgcag cactggggct cgcgctggta ctgctcagct actcgccgca
    40741 ggaccctggc tggacccaca gcggccaggg atcggtcacc aacctgggtg gcgcgcccgg
    40801 tgcgtggatc gccgacgcac tgttttcgct gttcggcttc tccggctact ggtgggtggt
    40861 gctcggactg gtcacgacct ggcgcggtat ccgcgaggcg cgcaaccccg ccgaacggcc
    40921 cgcgccatca tggcacgcct tcctccatgg tgcaggtttt gcgttgctgc tgctggcaag
    40981 cagcggtctc gaatcgatgc gcctgcaccg tttcgacgcc ctgctgccgg aacaggccgg
    41041 cggtgtggtc ggcacactgg aaaacgacct gatgctgcaa tggccgggat tcacctccag
    41101 cacgctcaca ctgctgctgc tcatggcggc aggctggagc ctgttttccg gcatcagctg
    41161 gttacggacc gccgagatca tcggcaccgc actggaacag ggctggatgt ggtttgccga
    41221 acgccgcagc ggatggcaga tcaaggccgt cggcgccagc gccacccgcc gccgcgaagc
    41281 cgtggtacag gaacagaagc aacgttatgc ccagcaggag ccggtcgtga tcgaaacgcc
    41341 ggtggtcagc atcgagaaat ccacccgtgc cgaaagcgag aagcaggcac cgctgttcgc
    41401 cgaactgccg gacagccagt tgccgccgct gcacctgctc gacatggccg gcggcccggg
    41461 cgaaggtgtg gcgagcgaaa ctcttgaatt cacctcgcgc ctgatcgagc gcaagctcag
    41521 cgaattcaat gtacaggtgc gcgtggtagc cgccctgccc ggcccggtca tcacccgcta
    41581 tgaaatcgag cccgcctccg gcgtcaaggg cagccagatc atcaacctgt ccaaagacct
    41641 ggcccgcgca ctctcggtgg tgagcgtacg tgtggtcgaa accattcccg gcaagagcgc
    41701 catggcgctg gagttgccca atccggagcg cgagatcgtg cgcctgtcgg agattctcga
    41761 atcacgcatc taccaggaca ccgccagccc gctgacgctg gccatgggca aggacatcgc
    41821 aggccatccg gtggtggccg acctggggaa aatgccgcat gtgctcgtcg ccggtaccac
    41881 tggttccgga aaatcggtgg cgatcaacgc catgatcctc agcctgttgt acaaggctga
    41941 tgcgcgccag gtgcgactga tcctcgtcga cccgaaaatg ctcgaactgt cggtctacaa
    42001 tggcatcccc cacctgctca ccccagtggt caccgacatg aaacaggccg cttcggcact
    42061 taactggtgt gtcggggaaa tggaacgccg ctacaagctg atgtcggcac tgggtgtacg
    42121 caacctggcc ggcttcaacc agaaactgcg cgaggcaagg aaatccggcg agacgctcac
    42181 caacccgttc gcgctcaatc ctgccgaacc cgagccgctc gaagaactgc cgctcatcgt
    42241 ggtcatcatc gacgaactgg ccg
//
