LOCUS       DAIDDT010000001        27654 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_1_length_27654_cov_6.631686, whole genome shotgun sequence.
ACCESSION   DAIDDT010000001 DAIDDT010000000
VERSION     DAIDDT010000001.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 27654)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27654)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27654
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1_length_27654_cov_6.631686"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         6647 a         6638 c         7739 g         6630 t
ORIGIN      
        1 attataagca gtgagggatt actaaaaatt attaattatt ataggatcta agttctatta
       61 aaaattatat gagcgtggca catccgacaa taaatcctcc agataaaagc ataaatcctc
      121 gccggagttt atcccgtgcc gcgatacggg acgaggacga ccgggtgaag acatccggtc
      181 aattcattat taccggaggg aagccgcttt acggcgagat cgcgctcgcc ggcgcgaaga
      241 acgcggtttc gaaaatgatg gttgcgtcgc ttttaaccga cgagccgtgc gttctcgaaa
      301 acgttccggc gattggcgac gtggcgatta tcgtggagct tcttgaacgg attggaagcg
      361 gcgtggcgat cgcggggcct tcggcgcgca tccatacgcc tgccataaaa aacagccgcg
      421 ttttggaatt gagccgccgg aaccgtattc cgattcttgc gatcggcccc ctgcttgcgc
      481 gcgcgggcga agcggaagtg ccgatcctcg gcggcgacaa gctcggaccg cggccggtcg
      541 acctccacct tgaagcgctc gcgcgtctcg gtgcgcagat cgagatcacg aaaacaagtt
      601 atcgcgcccg cgcgaaggac gggcttcatg gcgctcgtat tgcgttacgt tttccgagcg
      661 tcggcgccac ggagaacgcg atgcttgcgg ccgtgcttgc gaagggaagg accaccatca
      721 cgaacgcggc attggaaccg gagctcatcg atcttattat gatgcttcag aaaatggggg
      781 ctattatcga gcttgcgccg ccgcggacaa ttacgatcga aggcgtaaaa aaacttcatg
      841 gcgtgacgca tcgcattatc cccgaccgga acgaggcgat ctcgttcgcg tgccttgcgc
      901 tcgggacgaa cgggaaaatt ctggtaaagg gcgccgtgca ggagcatctc atcacgtttt
      961 tgaatgcagt gcggcggatg ggcgcggtgt acgaggtgac ggatgacggc atcgcgtttt
     1021 cgcgggaaaa tacaaacggc catgcgtcgc ttaaagcaat cgacattatg accgattcgc
     1081 atcccggttt tatgaccgac tggcagcagc cgcttgccgc cgtcttgacg caggcggaag
     1141 ggacatccac aatccatgaa acgatctatg aggaccggtt tgcgtacgcg catgacctga
     1201 atgagatggg cgcaaaaatg aaaatctcca tggactgtcc ggagggggat gcatgccgtt
     1261 tcggcagcgg gaaattttat cattccgcga aaatagaagg gccgacggaa cttaccgcgg
     1321 cgaattgcgc cgtgcgggat ctgcggtcgg ggatggtgga catcatcgcc gctttgatgg
     1381 cgaaaggcgt atccgtcgtt gacggcgtgg aagagattga tcgcgggtat gaaaagatcg
     1441 acgagcggtt gaggaacctg ggagcggaaa taagaagggt ataggccgcg tcacgaccgc
     1501 cttaaagtgg gaattcggat tgatatctgg taaaatccgg atatgacgaa gcctccgaaa
     1561 aatcaagtcc gcgtcagaat ggcgcccagc ccgacgggtt cgatgcacat cgggaccgcc
     1621 cgcgtggctc tttttaactg gcttttcgcg cgccaaaacg gcggcgtgtt tgttttgcgt
     1681 atcgaggaca ccgataagga acggtcggaa aaaaaatacg aggacgagat tttggaagga
     1741 ctcgcgtggc tcggcttgga ctgggacgaa ggactgggaa tagaatatgg aaataagaat
     1801 acagaagata atcatgaaga aaaaggaaaa aagaaccagc cggattccaa attccaagtt
     1861 ctcaatactc aatacttcgg acaatatggt ccttaccggc agagcgaacg gacggcgatt
     1921 tataaaaaat atctggagca attgatcgcg cagggcgacg cctattattg ttattgcacg
     1981 aaagaagacc ttgaggcgga gcggcaggaa ttggccgcgc ggggactgcc gccgaagtac
     2041 aacgggcatt gccggcgcct caaagaacct ccggcgggaa agaatccgga agtgatacgg
     2101 ttcaaagttc cggaaataaa aattgaattt aaagatatga tccgcgggaa cgttgttttc
     2161 gacgccgcgc ttttcggcga tatcgtgatc gcgaaagaca ccgcaacgcc gctttataat
     2221 ttcgccgtcg tggcggacga cgcggccatg gcgatcacgc acgtcatacg cggcgaagaa
     2281 catatttcca atacgccgaa gcagattctg atgcagcgcg cgctcggttt tccggagccg
     2341 atctacgccc atcttcccct gatcttgaat gccgataaaa gcaaaatgtc caaacgtttc
     2401 ggcgatgtcg cgcttttgcg ctatcgcgaa aaagggtatc tgccggaagc gctgctcaat
     2461 tttatagcgc tgctcggatg gcatccgaaa ggcgatcggg agattttttc aaaagacgaa
     2521 cttataaaag aattcgatat tgcgcgcgtc cagaaaagcg gcgcggtgtt taaccaggag
     2581 aaattggact ggatcaatag agaatatttg aaaaaaacgg acgcgggcgc gatcgcggat
     2641 atgcttgcgc cgctgcttgc cgaagaaggc gtgcgtccgg aaaggcaatt tcttgaaaaa
     2701 ataattgccg tcgtgcgcgg acgggcggcg actatccgcg acattgccga tacgggaaga
     2761 ttttttttcg cgctgccgga ttatgaagga acgttattga tatggaacga tgctgatgcg
     2821 ggcgatgtga aggcggttct cgaggaaacg gcatggaagc tcgcggaagt tccggatggc
     2881 gcgttttcgc gcgaaccgct cacgcgttat atcgcggaga tcacgggcgc gcggaaaagg
     2941 ggagcggtgc tgtggccgct gcgcgtcgcg ctttccggcc ttgcggcgtc gccggatccc
     3001 attgaaatca tggaagtgct cggcaaagat gaatccttgc ggcgcatcgg gatcgccgtc
     3061 cgaaaactgg aaaaatgacg atgcacggcg gcaataaata gaattttaga atagagaagg
     3121 aaatcaagcc gcaggcctgt ttttggacgg gtttttcaat tctgtttttg gtgtatacta
     3181 agaaagtaat gccaaagatc ttaaaaccga aggttttggg ggatgttgcg ccgtccgatg
     3241 tttcaggcag acaatggaac gttttcggtt cggcggccgg gcggattaac ttgtccgtgc
     3301 ggcgcgtttt tcgcgtcaat atcgtaacgc cggcaaaggc gcttacggtg gccgccgccg
     3361 ccattttttt cattttcgga tcggtgaatg cgccgtcgat tcttacgaag gcgtcagggg
     3421 atactacggc gaccacgaac acggcaaccg agcagcagca gcttcaaaac cagctccagc
     3481 agctggaagg gcagatcaat caatatgaaa atcagatcac gagttataaa aaacagggga
     3541 acacgttaag caaccagatc gcgaccctga acgccaatat cgcgaaggtc aatttgcaga
     3601 ttcaggcgac cacgataaca ctcagggaac ttgataagaa tatcgccgat acgcaatcgc
     3661 agatcgcggt gacgcagggc gatatcgcca gcaagaaagt ggcattggga aatcttttga
     3721 acagtcttta tcaaaccgat caggtaagtt tgatggaaat atttctcaaa gatccccgtc
     3781 tttccgattt ttggagcgat acgcagaaca tcgcgttgtt tcaggacaat ctgcgcgttg
     3841 cggtccagca ggtgaccgat ttcgcgaacc agcttcagga tcaggaacag caatttgcgg
     3901 cgtcgcgcgc cgatgcggcg agcgtgcagg cgtatcaggc ggcgcagaaa gaccagatcg
     3961 cgagcacaaa ggatcagaaa agcacgttgc ttgcggaaac caacggacag gaatcgaaat
     4021 ataaagttct tttgacgcag acccaggcga cggcggcgca gatccgggcg aggatattcc
     4081 aactgctggg cggcggccag ctttcgttcg gcgatgcgta tcagtatgcc aagcttgcat
     4141 ccaacgcaac agggatcgat ccggcgctga tccttgcggt gcttgaccgc gagtccgcgc
     4201 tcggccggaa cgtcgggcaa tgcaattata agacggcaat gagccccttg aaccagccga
     4261 tttttctcgc tatcacgaag gaattgaatc tcgatccgaa tacgatgacg gtttcgtgcg
     4321 ccaacagcga cggcgcgtac ggcggcgcga tggggccggc gcagttcgag ccgtcgacat
     4381 gggagcttta cgcaagcggc attaccggca tcacgggaca caatccgccg tcgccatgga
     4441 acgatgcgga cgcctttacc gcaacggcgc tttatttgaa ggacgccatg gcggggtgcg
     4501 cgccgcttta cactgcaagc ttaagccagg agcggtgcgt cgcggcaaaa tattacgcag
     4561 gctcccattg gcagtattat ctttggacgt acggcgaagc ggtggtcgaa caggcgcagg
     4621 gttttgcggg cgacatcgcg acgattacga aatgacgcat ccacctttta tgccgctcaa
     4681 aaatgggccg tgcgttatca tggaagccgt ttcggcgctc caatatccgg gagatatacc
     4741 gatgagcggc attgaatgcc ctttgttctc tttttttgac aagacattgc gggagagtag
     4801 aatggtaaga aatcaattct tcgaataatt atccttatgc cgcgcaaaaa gaacaatcat
     4861 ctcaaatata tttttgtcgt gggcggggtg atgtcgggcg tgggcaaagg cgtggcggca
     4921 tcgtccatcg gaaaaatttt acaggcgcgc ggattgcgcg taacggcgct gaagatcgat
     4981 ccgtatgtga acgtggacgc ggggacgatg aacccgacgg agcacggcga gacgttcgtg
     5041 ctggacgacg ggacggagtg cgaccaggat atgggaaact acgagcggtt tcttgaccgc
     5101 gatctttcgc gcgcgaatta catgacgacg ggcagtattt atctcgatgt catccggcgc
     5161 gaacggaatc tcgaattcgg cgggaagtgc gtttccgttg tgccgtatat cccgatggaa
     5221 gtgatcctgc ggatcgaccg cgcggcggcg aaagcggatg ccgaggtcgc gctcgtcgag
     5281 attggcggca cggtcggcga atacgagaat atcctttttc tcgaagcgat ccgcatgctc
     5341 cggctgcgcc atccgggcga tgttttgatc gcgctcgtga gctatatccc gtcgctcggc
     5401 gcgggaggaa acgagctgaa aacaaaaccg acccagcatg cggtgcggaa tttgaattcg
     5461 gtcggcctcc agccggatat tattttcggg cgcgcgccga tgcggctcga cgagaaacgc
     5521 aaggaaaaat tatcgcttat gtgcggcgtt gcaaaagaag atgtcatctc ttcgccgaac
     5581 gcggacagta tttatgatgt cccgctcaat tttgagaagg aagcgttgag cgaccgcctg
     5641 ttgaaaaagc tcaacctccg cccgcgcggc cgcgaccttg atgcatggcg gcggctttgc
     5701 cggacgatcc gcgttgcaaa gaaagaagcg aggatcggcg tcatcggaaa atatttcgac
     5761 accggagaat ttatgcttgc cgattcttat atttccgttc ttgaagcgat taagcatggc
     5821 gccgccgccg cccgccggaa gccggtgatt gaatggctcg gcagcggaga attcgaaaaa
     5881 gatccgcgat cgcttgcggc gctttcgcag tacgacggca ttgtcgtgcc cggcgggttc
     5941 ggccatcgcg gcgtggaagg caagatcgcc gcgatccgct attgccgcga acacaaaata
     6001 ccgtacctcg gattgtgcta tgggatgcag cttgcggtca tcgagttcgc gcggaatgtg
     6061 ctcaagctga aaggcgcgaa cacgacggag atcgatcaga cggcggcgca tcccgtgatt
     6121 gatatcatgc ccgagcagaa aaaattgatg gaagaaaaaa aatacggtgg aacgatgcgg
     6181 ctcggcgcat atccggcgga gctttttccg aaaaccatcg cggccgctgc atacggcact
     6241 ttgaaaattt ccgaacggca tcgtcatcgc tatgaagtga atccggatta tatcgggcgt
     6301 tttgaaaaag cgggcgtggt gttttccggt aaatcgcccg atggacggct tatggagatt
     6361 atggaattgg cgaaatccgt tcatccgttt tttatgggaa cgcagttcca tcccgaactg
     6421 aaatcccggc cgctgcatcc gcatccgctt ttcagggaat ttatgaaagc agcggcgaag
     6481 ggaaaaaaat aatgtgttat tgtcgcgtat tttctgtcat cccggaattt tgagtctgcg
     6541 aaaaatatca aggattcagg attaaccctc ttcattaaat taatcagaat tcaaattaat
     6601 tctggatccc ggatattcct cgcgaagcct cggaattcca ggacgacaga atgataatta
     6661 acttttcact tcttatgctt atctttctct acggtcccga cgattatcgc cggcataaag
     6721 cgaaaaagga aattatggtc cggtcggaga aaaatcataa cggcgcgcgg ccggcggttt
     6781 ttaacatgga aaacagggat gacgccgata atttcgaggc attcacgaga aaccagtcca
     6841 ttttcgaggc gacgaagtcg gcgatccttg aaaatgcttt tgttatggac gccgcaccgc
     6901 ttgcgaaaat attgaagccg cttgtcgcgg atgaaaatac caacgtcctt ttgtcggaaa
     6961 gaaaaaaacc ggtcaaagag ctcggtttcc ttctgaaaga gccggtcgtc gtccgggaat
     7021 tcgaaaacct tgccggaccg gagtggcttt ctttcataaa aacggaagcg aaagcggccg
     7081 gcgtcgcggt ggacgacgcg gcaatgcggt ttttgggcgg cgtgtacgcc ggcgattcat
     7141 gggcgctcgc gacggaattg cagaaattgg cgtctttcaa gccggcagtc ctgcggaaag
     7201 acctcgatgc gttcgatttt gagattgcgc cggattattg gtcgcttatg aacgggatga
     7261 aaagccgcga catccgcaac cggattatgg cgctcgaaaa acttttcgcc gcgaatgacc
     7321 ctgcggcaaa aacgtttaat atgctcgctt cgcaatggcg cgaaaaaatt ccgcagatgg
     7381 cggcgtatga ctttatggtg aaatcgggga agctgaatta cgaagaagcg ctgctggatc
     7441 tggtattgaa aaattaaaaa cgaaaaatga aaagtgaaaa gtgaaaaaag atcgaatttc
     7501 tgtcatcctc gacccggtcg aggaccaata ttttgaaaat tataaagaga aggacggggg
     7561 catggatttc ctccttcggg gggcgacgca ataaggcatt gcgccggatg ggaagcgtgg
     7621 gtcatccgtc ttcacggatg atgacaaggg gtatgggtcg tccgccggag tttatcatcg
     7681 gcggagctta tcccgtactt taatacggga atccttggcg gacgatgaca gaaaagatga
     7741 caagcggggg ggggcgaaag cggtaagcga caaaaaaacg gattcattgg aatccgtttt
     7801 tttattattt ttttgccgtc aattttgcga ttctcgattt cgtccgcgcc gctttatttt
     7861 ttgcgatgac gttcgttttc gccgccttat caagcgcctg ataaatatcg ggcagcattt
     7921 tttgggcatc ctcgattttt tttccgacaa ccagtttccg ataacgggta acgagctttt
     7981 tataagtttc ctttctcttc gtattttgga cgttgcgttt tttgctttgg cggaacgctt
     8041 tttttgcgga tttagtgata ggcatgtttt ttttgaggaa ttataggaat gaaaacacag
     8101 cattgagaag cacggagcga gacacggttt ttagctcctg attgttgatt tatggatatg
     8161 agtatataca ttggcggcaa aaaagcaagt tttcgattat aatggacgga tgatctatcg
     8221 cgtttcggga aaactggtgc tgatgtccga tcgttttgcg gtcgtcgaaa ccggcggcgt
     8281 gggatttaag atattggctt ccgcgcggac gcttgcggga cttggcgcgc cggcggcgga
     8341 cgtcgctttt tttacgcact tgcaggttcg cgaggacggc atggaccttt tcggattttt
     8401 gtcggaagaa gaactcgttt tcttcgagct tttgatatcc gtttcgggcg tcgggccgcg
     8461 ctcggcgctt tccattttgg acgttgcggg cctggaagag ctttcggcgg cgatcaaaga
     8521 aggacggccg gatcttttgt cgcgcgcgtc gggcatcgga aaaaagacgg cggaacggat
     8581 catcatcgaa ctcaggacga aagtgcagtc ggtgaaagcg ggggcggtcg tgcaaaaaat
     8641 ggaagtcgat gaggacctta tcgaggcgct ttcgaatctc ggttatcgcc gcgacgatgc
     8701 gcgcgccgcg ctcgcgaaag tcggtgcgga agcgcagggc atcaatgacc gccttcgcgc
     8761 tgcgctggga atattgggga agaagtgact ccgctttctt gcttgcgagg gacgggctct
     8821 cttaagtccc tcgcaagtaa gaaagcttcg tctatcatgg aaacgaagaa gggcttattt
     8881 tacttctctg tctttgccca aaacggaatc ctttgacttc ttcgtgaaca aaagaatgtt
     8941 tcgtatcgtt ataggatttg tgcatttgga aaaaatcata ataaaatgtc tcaaaccgca
     9001 acggataagg cccgtccctt ctttattttc cggttatttt catcgccgga gcagggctgc
     9061 gtcccggaaa ataaccggaa aatgccccgt acccggcacg agtgcgcaca tcctattgtg
     9121 tagaaatgat ggaaaataat cgcgaaaaaa caaaaaagaa gtttggcaac gttttttatg
     9181 tagggctttt gggttttttc ggcggcataa gccaggatat gttcgtccct attctgccgc
     9241 tttatctcgt gaacattctt gggttcagta cggaattcgt cggctttgcc gagggactgc
     9301 tgacggtcgg cgtgagcgcc ttccagttca tcgccggatg gctttctgat aagttcggca
     9361 aacagaagcc gatcgttttt accggatatt ttctctctat ggtgagccgg cccatgctgg
     9421 cgttcgtgac cggcggcgcg gcgattatcg gcctgcgttt tatggacggc gccggcaagg
     9481 gtataaagga ttcgccgaaa gacgtccttt tggccggttc caccgtgccg gaaaacaggg
     9541 gaaagggatt cggtatcgcg cgcatgcttg atacgttcgg ttcggtgatc ggccccatca
     9601 ttttattcgt cttgctttat cttttccgcg ataatgtatt gaagtatcat tatattcttg
     9661 ttttttgcgc cgttccgctt cttgcgacgc tcggcatcct tgtcgtaaaa gtaaaagagg
     9721 tttcgcccgc ggttcatccc gaaatttccg aagaaaaatc cggaatggca aaaaaagaaa
     9781 agagcgcgtt ccggcgggaa aagaacggaa aattacctgc aacattttat atttttctta
     9841 ccatcatcgg actttttacc cttagcaatt cgtccgacgc gtttttgatt ctccgcgcac
     9901 agaacgtcgg tgttacgttg cttgcgattc cgctcgtcta cgcgcttttt aatttcgtct
     9961 atgcgtccgc gtcggtgccg ctgggcagtt tgtccgaccg gatcggcagg gaaaaagtga
    10021 tcatcatcgg ttgggtggcg tacgccgtcg catacatcgg ttttgcgctt gcgaaaacgc
    10081 cgctcgacat ctggtttatc ttcgcttttt acggactcta ttacgcgatg accaagggcg
    10141 tggcgaaagc gttcgtcgcc gatctcgtcg gagcgccgca tcgcggccgc gcatacggga
    10201 tttacaacat gaccatcggt attttatcgc ttcccgcaag cgtcatcgcc ggatttcttt
    10261 ggcaaaagat cgatcctgcc gcgccgttct atttcggcgc catcatagcg gctatcgccg
    10321 ttgtgttgtt ggcgttgttc atgttttatt ccgcgaaagg cgcgcgccgc ggcggacaaa
    10381 ggaacgtcct ataggccgct ctttatattt tgaagcgggt tttgtgcaaa tcaggatgta
    10441 atacgttata atagaaaagt cgaaccgaag catttcatca taaaaaataa aaacatgatg
    10501 gcgcatatac cgttcaacgc ggataaaata aaaaaagaag acatcgacaa ggaaatatta
    10561 cggaccgcca ttatcgccga gctggatgcc gtgaatttgt atgagcaatt ggcttcagtg
    10621 accgataaca acgatctgaa aaaagttttc gccgatatcg ccggggaaga aaaaacccac
    10681 gtcggtgagt ttcaggccct cttattgaag ctggatcggg aacaggcgga agcattggaa
    10741 aaagggaaaa aagaggttga ggcattgacc ggataaacag caatcatcgc cgccatgtcc
    10801 ataaaaagaa aaaagatcac gagttcggcc tattttatag ggatgaacgg gaacggcatt
    10861 gtttcgttcg gctcgggcca gccggacctt ccgccgcctt cgacaatatt caagcatgcg
    10921 ggtgatgcgc ggcttttcag gtatgggctc atcaaggggg agattgatct gcggaaggcg
    10981 cttgcgaaac agtatcccga tgccgcaccg gaggatttcg tcgttacgaa cggcgcatcc
    11041 gaggcgcttg atctggtttt tcgggcgatc catcggatgc acgggaaggc gaaagtgctg
    11101 gtatgccggc cgtattatta ttcgtatccg ccgatcgtcg agcttgcggg gcttgagccg
    11161 gtctataccg acctcgaaga cggaaagatc aacctccgcg attttgaaaa aaaggtaaag
    11221 agcgttaaag cggtgttgat taattctccg tcgaacccga ccggccgcgt cgaagacctt
    11281 gcgacgctgc gcgcgataga aaaaatcacg gcgaagcgcg gcgtctacgt ggtttcggac
    11341 gaggtatata aagatctgat ttatgagcgc gagaattacc ttatcaaggg caggcacgtt
    11401 attacgatca attcattttc gaagacgtac tcgatgtgcg ggctgcgcat cgggtatctt
    11461 ttcgcgcgcg acccccgcat cgtggaagcc gttgtcgccc tcaaagtgca tacgtcgatg
    11521 aacacggatg tccttggaca ggagatggcg ctccgtgcga cgaaagtccc gaggagtttc
    11581 atcaaccacc agacagcgat atggaaacag cgccgcgatt atatttacgg cggactgcgt
    11641 gcaatgggat tcgacctttg gaagccggag ggagcgtttt atgtcctgcc gaagataaaa
    11701 aattcgggcc gcgttgtgga ggatttatat tacgatcaca aggttatcgt ctacgacggc
    11761 gcatggttcg gcgcgcccgg acggatccgc ctttcctatg cgcttgaact gcccaagata
    11821 aaagaagggc tttcgcgcat cgcgaaatac atcaaaggaa aagaaagctg gctgaagtag
    11881 gatttttatg acggtcgttc aggacaagcc ttcctgaatt ttttccagaa attttcttct
    11941 gaaaaatccc ggtgggcaag ggtgttttca tccgcgaccc ggaatcagga tataccagca
    12001 tcatcccatg aaaatacaaa acttcaagga actggcgaag agcgaactgc ggacggcggg
    12061 gctcagggtc gcggaggcgg ggcttcaggc gattgatacg gaagcggtcg tgcggcggag
    12121 cgtttcgtac ggcgcaggtg atctgaaaat tttcgacaag aattttccca tagcggagga
    12181 cggcaagttt atcgttgtcg gcgtcggaaa gtgcgcgctc gaagcggcgc gggcgctcga
    12241 aggcattatg ggcgatcgta tcacgaccgg cgccgtgatc gacgtccacg aagggatgct
    12301 caaaaaaata aaaacctatc gcggcacgca tccgtttccg acggaggcga atgtcgacgc
    12361 gaccagggag attatagatc ttttacgggg tcttacggaa cgcgattttg tgttctttat
    12421 catatccggc ggcggttcga cgcttctgtg ccagccggaa aatttcacat gcgtggacga
    12481 gcgggaattt ttgcgccact tattcgagaa gggcgcgacg attgaggaga tcaatacggc
    12541 gcgaaagcat atttcgcgcg cccgcggcgg gtatcttgcg gaagtcgcat atccggcgcg
    12601 cgcggtctcg cttatttttt cggatgttcc cggcaacaat cttgaattta tcgcatcggg
    12661 accgaccgtg cgtgatacga cgaccgtagc ggatgcgcgc gcgatcatcc ggaagtacga
    12721 cggcgaggaa cgttttgccc atatcgaaga caatctcatc gagacgccga aggacggaaa
    12781 atatttcgcg aacgtcgcga atttccttgc cgtggacaat atgacggcgc ttgccgccat
    12841 gcaggatgaa ggaaaaaaac tcgggtttgc cgcggccatc gtaacggcga cgctgcaggg
    12901 cgaggcgcgc gatatcggcc gggacgttgc gcgcgcaatc cgcggcgcat cgccgaaaac
    12961 aatattgctt tacggcggtg aaacgacggt gaccgtagca aacccgaacg gcgcgggcgg
    13021 ccgcaaccag gaagtcgtca tgggcgcgct gcccggtatc ggagaagggg agcttgtgat
    13081 gagcgttgcg acggacggga aagacaacac tggtttcgga ggggcgttat gtgatacaat
    13141 aacgaagcag gctgccctgg agaaaaatct ttcaccggat gtttttttgg aagagaaccg
    13201 gtcgtacgat tttttttcgg cggtcggcga ttatctcatg atgggggaca ccggttccaa
    13261 tgtttcggat atcattatta cgatcaggaa ataaatttcc ctctctgtca tcatccgtga
    13321 agacggacga cccgcgcttc ctgttttttc ttttaaaatt ttcaaggcat ggatccttga
    13381 cggagtttat cccgtaccac gatacgggac gaggacgacg gataggatag gatatggacc
    13441 atggcgggta tgcagcgata agaatatcaa aggcgaataa tgttttatat ataaagagaa
    13501 aggtattaca atataataat aaaataaaat ggcaaagaaa cttaccgttc cgttcgaaga
    13561 aatagggatg aaagacctcg gcattgccgg aggaaagaat gcgtcgctcg gcgaaatgat
    13621 ccggacgctg ggagcgaaag gcgtgcgggt gccgagcggt ttcgttatca cggccgaggc
    13681 ataccgctat tttttgaaat ccgcgggact cgatgttttt ataaagaaaa cactggaagg
    13741 ccttgatacg aaaaacctga aagatctcgc gcgccgcgcg aaaatagtgc gtgaaaaaat
    13801 aaaggcggcg ccgttacccg gtgaccttgt gaaagagatc aatgccggct atcgcgccat
    13861 ggaaaaacgt tacggcagga acatcgacgt cgccgtgcgc tcgtccgcga ccgcggaaga
    13921 tcttccggga gcgagttttg ccggcgaaca ggaaacgtac ctcggtattc gcggcgcggc
    13981 ggagctcgcg gttgccgcgc gcgcgacgat ggcgtcgctt ttcaccgacc gcgccatttc
    14041 gtaccgcgcc gacaagcatt tcgaccatct ggatgtcgcg ctgtcggtcg gcgtgcagaa
    14101 gatggtgcgt tccgatctcg gtgtttcggg cgtgatgttc acggtcgaca ccgagtcggg
    14161 atttccgaat gttatcgtca tcaacggttc atggggtctg ggcgaaatga tcgtccaggg
    14221 agaagtgacg ccggacgaat ttctggtatt caaaaagaat cttgcgaacg acgcgttttg
    14281 tccgatcatc gaaaagcatc tcggcgttaa aaataaaaag atggtgtatg cgcccgcaat
    14341 gcacagcatc aagcagacga aggttgtttc gacgagcatc gccgatcggc gcaattttat
    14401 tttgaccgac gatgagatcc tcctgcttgc gcgctggggc gctattatag aagagcatta
    14461 tacgaagcgg agcggccatt ggacgccgat ggacatcgag tgggcgaagg acggcaagag
    14521 cggcgagcta tttattgttc aggcgaggcc ggaaaccgtg caggcgagcc gcgatttttc
    14581 gaaggtgaag gaatatcatt taatgggatc ccgtactgcg ccgccgcttc ttcgcggcat
    14641 ttccgtcgga tcgcgcgtcg cgtcggggca ggtgcatatc attttgaatc cgcgggatat
    14701 cggcgctttc aagaaaggcg aagtgctcgt gacaaccatg accgatccgg attgggaacc
    14761 gattatgaaa atggcggcgg cgatcgttac cgataaaggc ggccggacgt cgcatgcggc
    14821 aatcgtgagc cgcgagctcg ggattcccgc ggttgtcggc accgaagcgg cgacaaaaaa
    14881 actgaagaac ggcgattgga taacgatcga cacgacggga agcgacggta ttatttacgg
    14941 cggaaaactt ccgttcaggg tcattcagca cgatctgaaa aaattaccga agccccgtac
    15001 cgaagtgatg atcaatgtcg cgatgccgga tacggcgttc gagaattcgt tttatccgag
    15061 cgcgggtgtc ggccttgcgc gcgaggaatt cattattgcg tcgaagatcg gcattcatcc
    15121 gctggccatg atcaatttcg cgacgcttcc agaacccttt aagcggcgca tcgaccggat
    15181 gacgaacgac gcgtccgaca aagtccagtt ttatgtcgat catctcgcgt atggcatcgc
    15241 gaagatcgct gcggcattct ctccgcgtcc cgttatcgtc cgcttttctg atttcaagac
    15301 gaacgaatac cgcgcactcg taggcggcga gctgtacgaa ccgtacgaag agaatccgat
    15361 gatcggctgg cgcggcgcgt cgcggtatta cgatcctaag ttcaagccgg cgttcaaact
    15421 cgaatgtctg gcgatgagaa aagtgcgcga gcgcatgggg cttacgaacg tgattccgat
    15481 ggtgccgttc tgccgcacgc cggaagaagg gaagagaacg cttgaaataa tggaggaagc
    15541 ggggcttcat acgcgcgcca tggcgaagac ggcggaagcg cggcggaagg cggtgccggt
    15601 ctatgtcatg tgcgagatcc cgtcgaacgt catccttgcg gacgaatttc ttgcgatttt
    15661 cgacgggatg tccatcggct ccaacgatct tacgcagtgc acgctcggcc tcgatcgcga
    15721 ttccggcatc gtggcgaagg tggcgaacga gaacaatccg gcggtcaaac ggtcgattga
    15781 gatcgtcatc gcgaagtgtc ttgcgaagaa aaaatacgtc ggcatttgcg gccaggcacc
    15841 ttcggattat ccagaattcg ccgaatttct cgtggagcag gggatccaaa gcatttccct
    15901 gaatcccgat accgtcgtgc cgacgctggt gaaaatatat caaaaagaaa aaaaattaaa
    15961 acgccgctaa aaaatgaaaa tagctttttt tgagcgcgaa gagtgggagg agtccgtcgt
    16021 ccgtaaaaat tttccgaacg atgaaatatt gtttttgggc ggtccgttga acgatgaaac
    16081 gattctgcgg cagacggatt atgacgtcat ttccacgttc gtcggctcgc gtatcaccgc
    16141 gaagacgctc gccatgttct cgaacttgaa attcatcgcg acccgttcga ccggttttga
    16201 ccatatcgac ctcgatgcct gcaaggcgcg gggcattacg gtcgcgtacg ttcccggcta
    16261 cggcgacaat accgtcgctg aatttgcgtt cggactgctg ctttctctta cgcgaaaaat
    16321 atatcaggcg gccaatcgga tgaaagaaac aggatctctt tcacaggagg gattgcgcgg
    16381 tatcgatctt aaagggaaga ctatcggcat tatcggcacg gggcgcatcg gccgcgaaat
    16441 gattgcagtt gccaagggat tcggcatgaa agtgctcgcg tatgatccgt tcccgaatgc
    16501 ctcgcttgcc tcgacgcttg gttttcggta cgttcccctc gaggagcttc tcgcgggttc
    16561 cgacgtcgtc tcactccatt gtccgcttac gaaagagacc atgcatatga taaacgcgaa
    16621 agcgatggcg cagataaaaa aaggcgccta tatcgtgaat accgcgcgcg gcggccttat
    16681 cgaaaccgaa gcgcttctca ttgcgcttga aaacggcacg gtcgccggcg tcgccatcga
    16741 tgttttcgaa ggcgagaacg agaccagaaa cgaattcgga tttttagaga gcgaggccgc
    16801 gacgaaggag gcgcttgaaa tcgtccttga aaacgaaatg ctgatgaaaa tgccgaacgt
    16861 gcttatgacg ccgcacatcg cttttgattc gaacgaagca ctcgcacgga tattggacac
    16921 caccatagac aatatcaaaa agtttatttc aggagaaccg gccaaccttg ttccgtagct
    16981 tcccattccc tccatcgtcc ttgacgccgg tcgaggatgc atgctttgaa aaatcaaaaa
    17041 gagagggctg gaagcataga tcctcgtccc gtgggatagg cgaagccgcc attccgcggg
    17101 gcgagccttc gcgagaacga cagaaagacg ataaagcaat aatccttatt ttttaaatca
    17161 taaatgcgca tcataaacgc caccgtgacg aaaattttcg actcgcgcgg cgagccgacg
    17221 attgaaatcg cgctggaagc taaagacgga accaaaggaa cggcgcaagt gccgtcggga
    17281 aagagccgcg gcacgggcga ggtaagcgtt gtttcattcg atcgcgctgc gcggacggtg
    17341 cgcggtatcg tgccggcgat gcgccgcgcg aatttttcgt cacccatgat cttcgaccgt
    17401 tttttgcgcg tgcaggacgg tacgcgggac ttgcatcgtc ttggcggcaa ttgcgcactc
    17461 gggctttcgc tcgcattcgc gcgcgcgatg gcggcggaaa ggaaacagga actgtgggct
    17521 cttatcgaag gattttattt taaagaacgg cctgccgcag cgcgtccggc gcgcccgatg
    17581 atcttttcga acatcatcaa tggcggcgcg cacgcagcga cgaaccttgc catccaggaa
    17641 tatatcgctg tcgcgaagcc ggagggggca tcctcggaaa cggttgcccg cgttatcgct
    17701 ttataccgcg aagtgggcaa tgcgctccgc cgccggacgg gattgcggcg tttgcctttg
    17761 ggcgacgaag gcggttatgc cgttgatttc aaagataacg gcgcaccgct tgcgcttctt
    17821 gccgcaagca ttaaaaaact ccgttttgat aaaatattct cgcttgcgat cgatgccgcc
    17881 gcgtcggaat tttataagaa cggaaaatat aatttcgaag gcaaaaaatt atcgtccgcg
    17941 gcgatggcgg acgtgtacgc ttcgtttcgc cgccgcgcgt cgtcgctcat gtccgtggaa
    18001 gatccgtgcg ccgaaaccga cgcgaagggt tttgcgctcg cccgcgcgcg attgcccggc
    18061 gtttggatcg tgggcgacga ccttaccgtt accgacgccg cccgcatcgg acgcttcatc
    18121 gaagacggcg tcattaccgg cgttattatt aagccgaacc agatcggcac gctctccgca
    18181 acctgtgacg cgatcacggc agcgcaaaaa cgcggcgcgc gcgtcatcgt ttcgcaccgc
    18241 tcgggcgaaa ccggtgacaa tgccatcatt catatcgcga aagcgtgttc cgccgacgcc
    18301 gtgaaaatcg gcgctcccgc ccgcgaacgg cttctgaagt ttaacgaata cgtcaggatc
    18361 ttccgttgaa accagatcgg cattttttct tttgtcgtcc cctcgaagga ggggatctat
    18421 gcttccaatc ttaaattcgg aaagcgtagg ttctcgcctt cgcgaggacg atagaggaga
    18481 cgaattccct ttttttgcga cggcattaca caggcttgct tgtttatttt atatgtgtta
    18541 atgtatcacc agttaatttt taaaatttaa taaaggaggt aaaaaatgaa cgagtgtctt
    18601 gatcttgatg gttcactgga tctgtttcat aattcagtgg ataaactttt cgatttattg
    18661 aaaaacggca ttaatgattc tcctgaccgg ctttacggtt cactgaaaca gtttgatgat
    18721 tcactaaata aactttcttc ttcagtggat cgactcagcg gtttgctgcg gaatcagaca
    18781 ttttcgtcgg caccgagccg aatcaatagt tcattggatc ggctttgcga aacattaact
    18841 tgggttgata aagcaccgga gttggatggg cttcgcgatt cgctgagcag ggtttatgat
    18901 ccgccgaaga tggaacgatt taatagcttg ttggacagaa tgaatgattc gatggataag
    18961 cttcgtaatt cacagcaccg gctttgcgac gtattggatc tattgaatag gcgaatgcgc
    19021 gatctgcata atcaggaatt agagcggatg cgcagacaaa aaagacttta ttatgagcaa
    19081 agttatcaac cttctcaccg taggatcaaa gagcaaccag aatgtgatga ttcccattta
    19141 ttatgctttg tgttagctct cgcaattcta tagcagtttt tagctgcttg cgttcatcgc
    19201 aagcagcttt tattttttaa tgaatacatt aggatcttca gctgaaggat aatttagtgt
    19261 tttttgtcat tttggaaaat gaaagctttg aatgtttatc cagcgttcgg ataatccttt
    19321 ttagaacatg gatcctgaat attttttgcg gatttaaaat ttcaggatga cagaagaaaa
    19381 aaagcggatt gcaaaagcaa aaaataaaac cccttttcgg ggttttattt tctattctgt
    19441 cgtttttccg tcaaagtggt cattttcttc gtctgccatc gcgtccgttt tcgcgggatg
    19501 aatggtgcca tccttatgct ggggcgggga gtagagcgta taaagtttca tcgttttgtc
    19561 gggcgatgtg ttttttacat tatgctccgt gcccgcgggc acgagtatgg cgaaaccgtc
    19621 gccgaccgga tgttccgcgc cgtcaagaat cgcggttccg gttccctcct cgatgcggag
    19681 aaactgatca acggtatgta cctcgcttcc gatttcctcg ttcgggagca ggctcatcag
    19741 gacgagctgg ctgttttttc ccgtgtagag caccttcctg aaatgcgcgt tgttttttga
    19801 ttcttcttct atgtttgcaa tgtatccttt cataggatta ttttttttat agttttaatt
    19861 ttttaaagag agcgaccttt tccggtattg taccacatcg ggaagattgc ggcgaaacat
    19921 tgcattttta cttattccgc ataaaatagg tattgttcac cgcaggaatt catccgcccc
    19981 tatagctcag gcgggagagc ggctccctct acctatctat aaaaataaaa attttatcct
    20041 atcggttcgc tcattaaaaa taaaaatatg atttcttctg ttcttgccat aatgccccta
    20101 tagctcagtt ggtagagcag ctccctctta aggagatggt ccgaggttcg aatcctcgtg
    20161 ggggcactat ggccggaacc atcatatttt tattctcaat tcactgccga tggtaaggag
    20221 atggtccgag gttcgaatcc ccgcggggcg ctatgatacg gcagtcagct gaaccgactc
    20281 tatattaaaa ataaaaaaaa ggagccctta tcgactgcgc gcataaggcg cagtgcgggc
    20341 tccttgcttg tatttaatgt cctttagatc agggacaggg aaccggcctc ccaaggtcgg
    20401 gaatcatatt cccgtccgca acttccggcg catagcattt gtttggaggt aaaaatcctc
    20461 ctggttgcgg cggttgcgac catgcgctcg tctcctcaat gggtggaact tcggtcacat
    20521 aatagaaccc gccgccgtta tacggtccat aatatgtggg taccgaaatt atgtaatatg
    20581 gccaccagca tatattgaac ccgcccacaa cggggattct gacgaggaaa gcgccaactc
    20641 tggcacccat tggcacctgg ccggtataat aataaccgcg atagccgccc ggtccgatat
    20701 agaacctgcc gccgttatac ggttgtccat aaccgttgcc gactcctcca tttatgagac
    20761 ctccgatgat tgctccgccg ataaacgcgc cgatcattgg cgcccatccc caatgggcac
    20821 gatgatgata tctacgatga tggccatagt gatcgcgacc atgataaaac cttgctgcgg
    20881 ctgtcgtaga aaaactcgta gaaaaacaaa gaacggacaa tatcattatc gccaagaata
    20941 ttcttttttt cataacatgc ctcctttttg cgccagcggg ctcccggatt atccgggttt
    21001 gccatagcgg acagaggccc cgtaaagagc cgctctccgc tacggcaaat tataagtcag
    21061 tattttaaag gtactgtgga aatcatagca cactgacata ttatagtcaa tttttttgag
    21121 gtgaattcgc gcgctaattg agtaatctct cgcgcctctc ttttctgtcg tcattgaccc
    21181 ggtcgaggat ccacgctttg aaatcttcgg attggatacc ctctttcaag ggaacgacgg
    21241 aggggaaaat gtttcgacag aatcggcgct gactatagat agtgaggggt tgctttttga
    21301 cataaacaaa tcctgaaata tctctctggc tgtggtatca tatttccatg acgccgccat
    21361 ccgacgaaaa gccttacgag aagcttccga ccaagaaaca ggtggtcgcg ggtttcgtgg
    21421 tctaccggcg gacggcggac ggtatcaaat ttttattgct ttatcgccgc ggcaattact
    21481 ggaattttcc gaaggggcat ttcaaatcgg gggagcggag catcgatgcg gcgcttcggg
    21541 agctcgagga agaaaccggc atcaaaaaat ccgaactgcg gattgtgccg aattttcgcg
    21601 cctatgaacg gttttatttc cgcgtcggga acgaggggat ttacgacacc gtcattcttt
    21661 ttctcgcgga gacccataag gccgacatca agatcgtggc gcgcgagcac agcggtttcg
    21721 catggttcct gtatcgtgat gcgttaaata ttgttggcaa aaaatacggc gatacgcgga
    21781 aagtattgaa acaggcaagc gactttttac aggaacgcag cgcgcgctat cgcggcacgc
    21841 gcaatcccgg ttccggagcg ggcaacgttc cggcaaagaa aaatcccgct gcataagcgg
    21901 gatttttaga aagcgaaatt ttgatgtttt tcgttttgca aggagatcct cgtcttcacg
    21961 aggatggcta aaacaaaagg aacgaattct atgactttac cgtttcagcc acctttttga
    22021 aaacatcggg ttcgttttcg gcaagttcgg cgaggatctt ccggtcgatg atgatatttt
    22081 tctttttcag cgcggcaata aaagcgccgt aggtcatccc ttcgggtctt attgcggcat
    22141 tgattttcac cgtccataaa gcgcgaaaat tacgcttttt ttctttgcgt ccctggaacg
    22201 cgtgcgtcca tgcgtgaagc agcgcttctt tcgccgcccg ttctttcgat ttccttcccc
    22261 agcgaaaacc tttggcgctt ttcagcacgc ttctccggcg tttattggcg atggtacctc
    22321 tttttactct cggcatggcg ttagtagttg attagttttt tgagcggata atcgagcgag
    22381 cgggtttttc gccggccttg tatcgcccgt ccgttttttc tcgttcgaaa atgatcgacg
    22441 gccatcgggc gccggaccat tttcccgttt ttcgttattc gtatccgttt tgaaacactt
    22501 tttgcgagtg acatcgtttt ggttggcttc tccttaaaga cagaaagaag tatacctgaa
    22561 cgttttatat tttgcaaata tttgtatgcg cccgtccgtt tttgagacgg attccggcat
    22621 actgcggaca tggaaatatc cgcagtatgc tataatgaat caattatgac gcttcttatt
    22681 aagaacgttc gtattgtggg aggcgccggt tcgcttcgcc cgcgctccga tgagacaagc
    22741 ggctcttcaa agacatccga tattttcgtt gccgatgacc gcatctccgc catagggaat
    22801 tttccgaata aaaaagcggc gacgattgtg gattgccagg gcgcgtacgc aagtcctggt
    22861 tttattgatg tcaatgctga atcggatcat tatttcacgc ttttcgacca tccgagtcag
    22921 gacgattttt tgaaacaggg cgtgacgacg attatcgggg gaatgagcgg atcgtctttg
    22981 gcgccgcttc tttacggaag ccttgaatcc ataaagaaat gggccaataa cgaaaccgtc
    23041 aatgttaatt ggcataccat ggcggaatta ctttccgcaa tcgaccggcg tccgttcggc
    23101 gtgaatttcg gaaccatggc ggggcacagt accatccgcc gcgcgattat aggcgatgct
    23161 atccgcaatc tcaccaggaa cgagctcggt gttttcggcg gcgtgctcaa aaaagcgatg
    23221 caagaaggcg cattcggcct ttcggcgggg cttggctatg tccatgcaag ggcgacgccg
    23281 tacgcggaac tgcgatcgct ttcgaatatt gtgaaggatt ttcacggcat ttatgcaatg
    23341 cgattgcggg attccgttcc gtttacggca tcggtggcgg aagcgatacg gcttgcgaaa
    23401 gaaacgggga tgccgatcat catcaataat ttcgcgccgg tgacccgccc gaatttttcc
    23461 gaaaaaaatt caggaggaca cgccggcata acgcaggaat atgccgcggc gctcgcgctt
    23521 atcgacggtc ttcccgacag cgttgatctt tatttcgaca tcagtccgtt tcccgcggcg
    23581 ctcgcgccgt tttatacttt tcttccgttg tgggcgcaga cgggaggatg gaaggcgatg
    23641 ctccgtaacg tacaggacga atggcttttc ccgcgcatga taaaagacat gccgccgatc
    23701 gacgaggatc atttcacgat cgcgcaggcg ccgagcaata attttctcgt gggaaggact
    23761 cttcgcgaca taagggaaat gtacgaagtg aaggatgcgc gcgaggcgct cctgcatctt
    23821 atggcggcga tggatatcca cggcgtcgcg ctctatggaa atatcgacga aggcctgctc
    23881 atgaaagcga ttgcgagcaa aagaagcctc atcgtttccg gcgcgccgag tttcggcgga
    23941 acggcaaaga tcgcgcagct gagatcgaaa cggacaacgg aaacgttcct gaaattcctt
    24001 tcgcttgccg ggcatgacgg cattatgccg cttgacgagg cgatacggaa aataacgcgg
    24061 acgccggcgc ggaaattcgg ttgcgccgga cgcggtgaaa taaaggaagg caatttcgcc
    24121 gatatcgtat gtttcaggga cggcatgata aaatatacga tcgtgaacgg aaaagtcgcg
    24181 atagccaacg gcgagttcca aaatgttttt gcgggaaagg cgttgcggca cggaaaatga
    24241 agacgaccga taagtcaacg ctatctataa tttcataatc ttttaatcat ggcattgaaa
    24301 aaaaaaggac atgctccgga ttatttcctg atggcgctcg tggttgcgct taccgttttc
    24361 gggctggtca tgctcgcgtc cgcgtcgtcc gatctcggaa aggtgcaatt caatgacagt
    24421 tattattatc tgaagcatca gattctttac ggtctttcga tcggtatcgc cggattttgc
    24481 tgcggttatt tcatcccgta tcaaaaattg aaaaagattg cgctgccgct tctggttgtg
    24541 agtattattt tccttgcact tgttttcaca aaacttggtt cccttgtaaa cagtaccaac
    24601 cgctggctcc ggttcggccc tttgagtttt cagccggcgg agctgatgaa attcacgtat
    24661 cttacgtacc ttgcggcgtg gcttagcaac gtaaaaatga aaagagcatc gaatttcctc
    24721 gaaggactcg tgccgtttct tgccgtttcg ggcgtcatcg ccgcattgct tattctccag
    24781 ccggcgacga gcaccgtttt cattcttttg ggatcggggc ttgttgtata tttcatgagt
    24841 ggcgcgccgg tgaaatatat atttggaact attctcatcg ccgtgctggc cgtcggagcc
    24901 gtgatcatgg tgacgccgta ccgccttgcg aggattacgg ggttcctcca tcaatcccaa
    24961 aacacgcagg ggcagaacta tcaggtgaat cagggtctga tcgcgatcgg ttccggcggg
    25021 ttcatgggac ttggatacgg tcaatccgtg acaaaagggg gatatcttcc gacgccgatc
    25081 gacgattcca tttttgccgt gatcgcggaa gagctcggct ttgtgggcgc cgcttcgctt
    25141 atcgtgcttt tctttttgtt cacgatacgg cttttctggc tttcgaaaaa aacaggcgat
    25201 cgtttcggga aactgctgtt gatcggtttc ggaacggtgg tcgcgctcca ggcgttcgtg
    25261 aacatggcgt cgatatccgg cgtgatgccg ctgaccggtg tgccgttgcc gttcataagt
    25321 tatgggggta ccgctctggc ggtatttctt accataagcg gcgtcgcgtt gaatgtgacg
    25381 aaatacaact gaagaggttg taatgatgca gtgttctaat gttataatat tccaatggtt
    25441 gagcgccgta ttttcattac aacattacaa cattaaaaca tgtctgctat tcgtatcgtt
    25501 cttaccggtg gcgggagcgg agggcatatc tatcccttgc ttgcggtttc ggaaagtatc
    25561 cggaaatggg cggccgatgc gaaccggagc gttgcaatca cctatatggg tcccagggat
    25621 tcttacgccg atctgttcgc ggagcgcggc attgcggtgc ggcccgtcgt ttcggggaag
    25681 atccggagat atttttcgtt catgaatttt cttgacgtgc cgaaattttt tcttggattt
    25741 ttcgaggccg ttttcaagct ttacttcatc atgcccgacg tcgtgttttc gaaaggcggc
    25801 acgggcgcgc tggccgtcgt ccttgccgcg cgtttttatc ggattcccgt cgcgattcac
    25861 gaatcggacg taaaaccagg tcttacgaac ctcgtatcgt cgcgtttcgc aaaaaaaata
    25921 ttcgtaagtt tttccggcgc ggcggattat ttcgatccgc ataaaacgga ggtcacggga
    25981 actcccgtgc gccgcgcgct tctggaaaac cggagcacaa aagatgccgc gcgcgaaacg
    26041 ctcggcttcg cgtcgtctac gccgcttatt tttgtctatg gcggatcgca gggatcgtcg
    26101 aggattaatc attgcatcct tgaaaatctt tccgtgctta tccgggaaac gcagatcctg
    26161 caccagaccg gcacggcgaa ttttcccgaa gtggaaaaac tttcgcgggc ggcgctgatt
    26221 gacgcgtcgt tcaagaaccg ttatcgcgcg gtgccgtatc ttgccgatga tctccgcgcg
    26281 gcgttcgacg ccgccgattg catcgtcgcg cgcgccggca gcgcgacgct tttcgagatc
    26341 gccgcgttcg gagttcccgc gatttttatt ccgcttgccg aatcggcgaa cgaccatcag
    26401 aaagcgaacg cgtacgcgtt cgccgaaacc ggcgcggcgg tcgtcatcga agaatccaat
    26461 cttttgcccg gtatcttcat caatgaagtg cggcgcattc tttccgatgc gtcgttacgg
    26521 gagaaaatgt ccgcggcggc gaagcggttt tttattcccg acgccgccga taaaatcggc
    26581 gaggggatat taaagctggc gataaaccgt tcgtgacgga ttttacccca tcctggcact
    26641 tcaccttacc gttatagata ataagcaatg cgcaacaagt aggaaaaata ggaagtgtgg
    26701 gttataatgt ttttagcggc ggcgcgcaga accttcattg caataatgcc ctctataatt
    26761 gaagtcaaaa acctggtcaa aactttcggc gatttcaccg cggtgaacga tgtttctttt
    26821 gacgtgcaaa agggcgagat tttcgctttt ttgggaccga acggcgccgg aaaaacgacg
    26881 acgatcaaga tgctgacaac gcttcttaaa ccgacaagcg gagaaatttt ccttgacggg
    26941 aagaatatca tccatgacca ggatgcagcg cgccgttcgt tcggcatcgt ttttcaggat
    27001 ccaagtttgg acgacgagct taccgcgtgg gaaaatatgg aatttcacgg cgtgctgtac
    27061 catgtgccga aagcgatgcg gcggaaaagg atcgaagagc tgatgaattt cgtcgagtta
    27121 tgggatcgta aagatgcgct cgtgaagacg ttttccggcg gcatgaagcg ccggctcgag
    27181 attgcccgcg gtctcctgca tcatccgaaa gtcctttttc tcgacgagcc gacaacgggg
    27241 cttgatccgc agacaagaaa tcatgtctgg agctatattg agaacctcaa caaagaagaa
    27301 ggggttaccg tgttttttac tacgcactac attgaagaag ccgaacgcgc cgcgacgcgg
    27361 gtcgccatta tggatcacgg agttatcgtc gatcgcggaa cgtcggatga actgaaacag
    27421 cagacggaca gttcttccct tgaagacgcg ttcatcaagc tgaccggacg cgacctgcgg
    27481 aaagagtcgg cgggagcgga tgcgatgcga acgcacgcga aagtgtgggg gagaagataa
    27541 aaggcgtgcc gtcgcggcac gtcgtccccg cggaggcgag gtccatgctt tgaggaaata
    27601 aaagacggga agcgcgggtc gtccgccttc gcggacgatg acaaagaaca agtg
//
LOCUS       DAIDDT010000002        26689 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_2_length_26689_cov_6.292800, whole genome shotgun sequence.
ACCESSION   DAIDDT010000002 DAIDDT010000000
VERSION     DAIDDT010000002.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 26689)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26689)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26689
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2_length_26689_cov_6.292800"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    239..248
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6583 a         7120 c         6392 g         6584 t
ORIGIN      
        1 acctgattct gcttcagatt ccgatcataa tacgctccaa aaataatacg cgcgccggga
       61 tccgcggtct gcgcaaccac ctttgcgata tcgttcactt cggtcatttt gagatcgcgg
      121 ccgcccgaaa taccgagcag caccgccttc gcgccttcag gattcgtttc caaaagcggc
      181 gaatgaagcg ccatattaat ggccgcgacc gcgcgatcct ggccggacgc aattcccann
      241 nnnnnnnncc ttcgggattc acttcgagca acggagagtt gagcgcctgc tggaccgcgt
      301 ttaccgccct gtcctgcccg ctcgcaatgc ccacgcccac gatcgcaacg ccggcatctt
      361 gtacgacatt cttcacgtcc gcgaaatcga cgttgatgat gcccggctgc gccacgactt
      421 caacaagtcc gttcaacgca ttacggagca cttcgtcgat cgcctcgaat gccttcaaaa
      481 tcggcgtgtc cttgccgata atggaaaaaa tgcggtcgtt cggaacgaca atcagcgcat
      541 cgactttgtc ctttaatttc ataagtcctt cctgcgcaat ccgctcgcgc tggcttcctt
      601 caaaactgaa tggtctcgtg acgaccgcta tcgtaagcgc gcctgattgc ttcgccgctt
      661 ccgcgagcac cggcatcacg ccggttcccg tgccgccgcc aaggcctgcc gtaagaaaaa
      721 caagatcggc tcctttaacc agttcaataa gctccgattt attttcctcc gccgcctgcc
      781 gcccaaggtc ggggttcata cccgtcccca atcccttcgt cagatttttc ccgatgtata
      841 atttctgctt gacattgcac tggtcgagat cctgatggtc ggtattgatg gcgacaaaat
      901 caacgccgcg gacgaaatcc tttgccatgc gcgagacggc attgccgccg ccgccgccca
      961 caccgatcac tttcacgttc gccgaaagcg gtctgaattt ttctccggta ttttttttgc
     1021 gaacaacctt gcgaacggtt ttctttttca tttctttaaa ttatagcaaa acgattggtt
     1081 cccgcaatag cgattatcag tcatcctcgc cctctcaagt ttttctccga aaaattttag
     1141 cgggagatga tccatgcttt aagaaagtaa gatgggaagc atgagccgtc caccggagcc
     1201 tgcccttgac cacaatcgag aacggacgat gacagagagg ataagtaaaa gtttttacgg
     1261 cgcaaagtac cttatgaatt tttttatgcc gtgcatgctg ccgaaaccag tttgcagaag
     1321 ttccggctgc gccgattttt ccccgtaaat cccccataga gcaaggccga acgcgcacac
     1381 gaactcggga tcctcgagcg actccctgaa cgcccctcct tcgttctgcc attcatcggc
     1441 gacacagcat cctatctgcg aggtcatctt cacttcctgt ttcacaagat ccgcgaggcc
     1501 cggcattttc acgccgccgc ccgcgagcac gatgccgccc ggcagattgc cgtatttccc
     1561 cgcgatccgc agttcgttat tcacgaagtc aaaaatttct tcgaggcgcg attctattat
     1621 ttccgcaatg aaacggagcg aaaccgatcc cttcgcttcc ggaatgaatt ttttgagttc
     1681 gatggaatcc cgcgcgccca cttctttcgc cagcgcacag ccataatgaa gcttgatact
     1741 ttcggcggca tctatcggaa tcttgagacc aaccgcgacg tcgtttgaaa tattcgccgc
     1801 gccgaccgga aatttcaccg caagcaccag cttattctct tcatagacgg ccagtcccgt
     1861 cgtgccaaag ccgatatcga cgagcgccgt ccccagttct ttctgtttcc tgctcaaagc
     1921 cgcgcgggcg gcgacgagcg gcgaaaaaac aagaccgccc acgtcgccgc ccgcaagctc
     1981 caccgcgtga atcagatctt tcacgtgcgg cgaaaaggca tcgataatta aactctgcac
     2041 ttcaagacgg cttcccgtaa gactcaaagg atctaagata tcacccatgc cgtccaccat
     2101 gaattctttc gtaagcgtgt gcacgatcat ccggttctgc ggtaagttga ccgcctgact
     2161 cgcccgcacc gcgcgatcca catccgcctg ataaatctgc gcatccgcgc tcgagaccgc
     2221 aacaatgccg cgcgaggatt gcatcttcgc ctgcgaagtc ccgatactca gataaatatt
     2281 cctgagcgcc gcttttgaaa ttttttccgc tcccgcgagc gcacgccgta tcgcttgcgc
     2341 cgtttcggcg atgtccacga ttgcgccgtg ccgcatgccg gccgacggtt ccttggcggc
     2401 atataatagc gtagcgcgtc cttgccctcg ccgcgcaaca acgattttta tcgaagaggt
     2461 tccgatatca agtcccgtga taatgtcgtt atgggcgatg cctttgaact tcatgccgta
     2521 attatatcaa atggatgcaa tttttcaaaa ccgcaacggt gcgccgcctt cattcaaatt
     2581 cgatttgccg tcctcgtcct atattatcgc ggtgcgggat aaattccggc aagcccgcgt
     2641 tcaagttttt ctccaaaaaa tttcggcggg cgtcagatgc ccgaaaatac ttttttttaa
     2701 aacggcttta aaaaaagtat tttcggaagg aaagaaaaga tatcctgcga ataattcagg
     2761 atgtctttcg cggacagtga gaccgtcaat aaccaaaaaa aacgttctgt tttcttcgtg
     2821 ttttgcacaa ccgaagattt tatcaaagct gcgcaagaat ttttttctcc agccgttcca
     2881 ttttttcggc ggacgatttt cgtccatggg aatacccgag aagatgcagg atgccgtgga
     2941 ccaaaagcgg cgccgcgcgt tcgggagatt tcttcaggat atctttattc agatatatct
     3001 cgccgagata ttttttttcg gtttccggat gcgggaacga atgcggctcg ggaaaagaaa
     3061 gaatgttcgg ttccgttttc ttttttatga atcgcgcttt caaacgcgct atgtcatcgg
     3121 ttttaagcag aaaaatatcg agtgtaacat tacgctcgcg cattaatgta agtattttct
     3181 cggcaagaag atgaaggcgg cgttcggtgg tcatacgacg aattattgca gctttggaaa
     3241 attatcgaaa atcgattctt ctctgtcgtc cccgcggagt caaggatcta tgcttcccat
     3301 cttttgtctt aaattttcaa agcgtgaatc ctcggccaag ccgggaacga caaagaaaaa
     3361 cttttaagga acccttacaa caatttttct tttattatct cccccaccag cttcccgtcg
     3421 gcgcggcctt tgaccgtttt catcgtctcg cgcatcagcg cattgaaatc ggaaaatccc
     3481 tgcgccatcg catcatcaac aattttcgca atcgccgcgc gggaaagttc ttcgggaaga
     3541 tacgttctga taatagcaag gcctgtttct tctttctcca caagatcgtc ccggttcccc
     3601 tgccggaaaa gctcgatgga ctccttccgc cgtttcgctt ctttttggag cacggcaatc
     3661 gcttccgcat ccgtgagcgc cgcgccgggt tcctttgctt ctttttcttt ctgagcgccg
     3721 ttcaagccgg aaaggatgaa acgtaatgtt tcaagacggt ccttttcgcc cgccttcatc
     3781 gccgatttca tatcctccct gatctgttca taaagcataa aaaagttaat gacgaaaagt
     3841 ggatagtgaa aagtgaatga atgcgcgaag cgaataccta cgtttttagt ttttcacttt
     3901 tcatttcttt aaaccaaccc cagcttcttc tgccgttcaa gttctttttt cttcgcgtcg
     3961 cgatgaattg ccgtcaactt tcttttcacg cggctcgtgg cgcgcgtatg gaacttcctc
     4021 cggcgcattt cgcgcaaaat accgctcctc ttcacccgtt tcgtgaaact gaaaagcagc
     4081 gaatttcccg attccccttc tttttttctt acttccacgg ccataataag aaaagtgtag
     4141 cggaaaaata ttttttgcgc aatagcgcgc gctcatcgca tggcaatagc ggcatagtaa
     4201 gatgccgttc ctctttgaga gaataaagtg acaaaaacct taaaatgctt ttatcaaaac
     4261 aactgttttg ataaaagcat tttaatctgc gaaccatcag aagatcttta tgcgcacgtt
     4321 ggaaaatttt tcagaaaaag catatactca tttcgttgcg gcggcgcaaa acagcattta
     4381 aaaacatcct ccgatgaaaa acatcccgaa tcatatcgcc atcatccccg acggcaaccg
     4441 ccgttgggca aaaaaacacg gcatcatggc gtggcggggc gtcgaggaag gcgcgaaacg
     4501 gttcgaggaa accgccgaat tcctgttcgg ccataacgtg tcctacgtca ctttttgggc
     4561 gggatcggaa gataacctca cgaaacggag cagaatcgaa gtgcgatttc tggtctccct
     4621 cctccataac tggcttttaa aaatggcggc attagatacc ttcgtcaaaa acaaaataaa
     4681 attcagagtc atcgggcgct gggacacgat ccttgcgaaa gacaaaaaaa cggccgcgct
     4741 gaaaaaactt atccgcgggc tcgagaaaaa aacggaacat ttccacggga aaaatctaac
     4801 cctactcttc ggctatgaca gccggcagga aatgatggag gcaatcgaaa aaataaagaa
     4861 tggcggcgca aaaaaagtgg atgagcgcgc cgtacgcgaa gcgttgcgga ccggcatatt
     4921 gccgccggtc gacctcgtca tccgcactgg cggcgaaccg cactggtcgg cgggattcat
     4981 gatgtggcac gccgcaaacg cccagctgta tttcacccgg acgctctggc ccgatttcaa
     5041 aaaaaaggaa acagaagcgg cggttgaaga tttctcgaaa agggaacggc ggtttggaaa
     5101 ataaaaatat ggctgtcatc gtcagccctt gatcgcagcc gaaggcggac tccggcagat
     5161 aatcgcgcgc tgcccgtctt attcttcaga gtgcggatcc tttgcccgcc ggaatttttc
     5221 tccgaaaaat tcaggcgggc tcggccggat ccggaaacga ctgaaatatt tttcctttca
     5281 ccgcgaaagg aaaaatataa cgagccccag ggcgcaggac atcgcggaaa taatccagaa
     5341 ccgcatcgtg atctgcgact ccggccaacc caaggcctcg aaatgatggt ggattggcgc
     5401 cgaacggaaa acttttttgc cgcggaattt tttggaaacg atctggatga tcaccgatgc
     5461 cgactcgata acgaggataa gaacgaaaaa tggaagcaac gcggcggtgt tcgtgagcat
     5521 cgcgatggcg ccgatcgtaa tgccgagaga catcgaaccg gtgtcgccca tgaagaattt
     5581 tgcaggataa atgttgtacc acaggaacgc gagcagggcg ccgatcatcg cgccgccgaa
     5641 cgcggcaagg tcataccggt gcagcacgaa cgcgaccacg gccagtgcga caaacgcgaa
     5701 aagcgacacg ccgcccaaaa ggccgtccaa tccgtcggtc tcgttcgccg aaaaagcgga
     5761 agcgaaaatg ataaaaataa agatcgggat ataccatgcg ccgattgtga aattcccgag
     5821 gaaaggaatc tgtatcacgt cccacccaag acggaaataa aaccaccacg cgccgacggc
     5881 ggcgagcgcg atgtaaatga tcgatttata gcggatcttc agtccgccgc ccgatgcgcc
     5941 acccattttc aaaacgccga cccagtcgtc gatcagtccg agtatcgcgg caataaaaag
     6001 cgcggcgagc ggcagatagg tttccgcgcg gtttataaaa ttgagatacg tccacgggcc
     6061 gtcaaaaaac cggctcataa cgccgaacag gatggcaagc ccgagcacgg ttccccagat
     6121 aatgacgccg cccgcggtcg gcgtgcctgt ttttttctta tgcagttcgt aaaaaacagg
     6181 cgtatccttt tcctcacgca actgcttccc gaaattatgc tttttcagaa aacggtacca
     6241 gagcggcgtc acgaaaagcg ccacgacgaa cgcgatagcg gacaatagaa ggaccttaat
     6301 tgcttccgtg aagatcatag ttatttcaca ttgctttcga attacttaaa agtaaaattc
     6361 aatagcgaag atttggacat tataaaatac tttcgagcaa aaaccaagaa cgcaaacact
     6421 atctttctct cctgttatta tgcgtcatca cgcaaaatgc aatagcgcgg gcgctacttc
     6481 gcctggcctt tgaaccaatc gtacagcttt tccgtattcg cgaaacgatc ggcctcgttc
     6541 gtgccaagca cgataaccaa aatcggacga ccattgtcgt taaaaatgga aagtagattg
     6601 ccgtccgcgc tgttcgtata gccagttttc ccgccgataa aatccgattc gccggcgaaa
     6661 tcattgatac tttttacgga tgttacggtt cccgtattga gctcggtggc attgaactgc
     6721 gccgtacgcg tgatcgcaag gatttccgga taatcggaat atattttctg cgcaaggaga
     6781 agcagatcgt ccgcagtcga cttattattt cgcgaaaggc cggaaggatc gtcgtaatgc
     6841 gtactcacca tcccccacgc aaccgcccgc gcattcataa cgttaatgaa attcgtccgt
     6901 ccgcacacaa ggcgtccgca cgcatcggcc agcgcttccg ccgcgacatt actcgaaggc
     6961 ataagcaaaa aatggagcag atcgaacacg gtatatacgt cgcccacgcg gagcgttttt
     7021 tcttccggat acgccgcgaa cgcatcgggc gtgatcgtca ccttctggtt catgtcgaga
     7081 ttatccatca ccaccgtcgc cgtcataagc ttcgtcaccg aagcgagcgg ccatcgcttg
     7141 gcgtcattat cgccgaaact tatcgcgccg gtctgaagat cggcgaccag tgccgcttcg
     7201 taatgaagcc ccgagagagc atttatattg ttttgcgcgg tggaagaatt tccggaagcc
     7261 gcgccggaat atcccgcagc tgcatccggc acggaagcgg caaaggcgct cttatccgcg
     7321 ccggagatac ttccatggga aaacgcatcc ggcacagcgc cggatccgga agcgacattc
     7381 tccgaaatca tcgcaagcga acccgccgcg gctcctttcg ccgccgcagt attcgagttc
     7441 atttcatatg ccacgcgcga aaccaggccg acagcgataa tggcgataaa aaaaatcaat
     7501 ttcttgtaaa aatctttcgt atttatcatg attgcggcga cggcggcgcg cttgccgcgt
     7561 cgaagatatt caacagctcc cggaattttt catagtcggg aaggtcgtcc cttttttgta
     7621 ctcccaaatg cttcatcagg tcaaatgtcg cgcgatacaa aaagcctgac gcacggtcgg
     7681 gatcgggaaa acgttcgaca aggccgcgca tcgacaagct ccgcagaatc acgctcgaat
     7741 tcacgccgcg gagatattct atttttgcgc gcgagagcgg accgaggtac gttacgatcg
     7801 caagcgtttc ggtgctcgcg ggcgtaagat cctccgtcaa cgcttccttt acgaatcccc
     7861 cgacgatcgc gttccactcc ggcttcgtcg caagctggat cttttcgccg tccgaaaaaa
     7921 gcatgagtcc gcgcgcggga tcatccagtt ttttcttcaa tacctcgata accgcatcgt
     7981 aatctccttt ttcccacgcg agcgccgctt ctatcttttt ccgcgcgagc ggctcaccgt
     8041 gaataaaaag caacgcttcg agtgacgcaa gttttttttc gagatcgtcc ataatgtaat
     8101 ttatttcttc tgttattccg gaaaacgctg gtatgcgagc gtttatccac gatccgggtt
     8161 tgtatttaat ttgaattgga tattgaacag aaaaacatta atgaatctgg atcctgggta
     8221 aatcgcgcag acgcgatttt ccaggatgac agcgcggatc agaaccgggc tgtcactttt
     8281 ttttcaccat tatatcagaa aaatttccga tttgctcaag ccggacgagc tgttcgcgcg
     8341 cgagatgcag caccgcaagg aaaacaataa tcatctccga gcgggactgc tcgccggaca
     8401 tatttttgag atcgaacccg ccttctttta tgacgcgcgc aaggacttcc tgtatctttt
     8461 cctcaagcgt gacaatcacc cccggtatcg tctgctcttc gcgttcgtag gttttgatcg
     8521 cttcgaaaat attcgagaga gcatttccca agtgatgcgc atcggtcttc gcaccgggag
     8581 aaaaaacact ccggccctgc gcgggccacg tgccgcgccc gagaaaatag ggcctgccgt
     8641 aagcgcgatg gccttcccgc cagagtccgg cgatcgccct gaacgccggt cgcaggtcgc
     8701 ggtagatcga aagccgcgtt tccaaatctt tgatgtccgc ttcttcttcg acggtaagcg
     8761 gcaaattcgg caggagatat ttcgatttca aaaaaataag cctgcttgcg accgctatga
     8821 aatcggcgag cagccgcagg tcggcgcgga acgcttgctc gttctccgaa agctcaccct
     8881 ctgcagtgtt gtttgataaa atttggaagg gcgggtctga cgaaccatcc gcaacgttgc
     8941 tctgtaaaat tccggcggac gaaacgcgcg aaagcggatc cgcatcctgc ttccggattg
     9001 cgtcaaggta tttcaaaaaa tcatccgtta ccgcggccat gcttacctcg ttcacgtcga
     9061 gtttctggtc ctcgataagc tcaagaagtt tttccaacgg cccgctgaat ttttcaagcg
     9121 tgagttcgta catggataat catagcgaca ctttatgaat aaacattatc tgtcatttca
     9181 ttatagaaca aaaaactttt tatttacgac gacgcatagg agaaaaatca gccttccaca
     9241 aaacaccgtg gccgcatgct catgcataac atcattcacg cgagaaagtc aatgaaaacc
     9301 gcgtccttga cttttttcat tgcgcaggtg tataataccc ttaatgtaag tgttctcggt
     9361 aagtttcgcg cgcgtattcg tcagagtatt gcggcgtccg tctcaaaaag aggcggataa
     9421 acccgccaaa ggggagaagc gtcgaagaag caggtcgcct tacaggatat tagcaattta
     9481 gaacttacga aattggatgc agttcgagga gcgcaaggaa aataacgcga gcaatatgct
     9541 gatattgtga gcgctatttt gcgtagcgcg acaaagaaat gcgccaattg cgtaagttct
     9601 ataaatcata tggcaacaaa gttatacgta ggtggtttgc cctactcgac gacgcaggac
     9661 gagcttcaaa acgcattcgc gaaagccgga accgtaacgt cagcgaacat catcatggat
     9721 aagatgacga atcgctcgcg tggattcggt ttcatcgaaa tggcgaccga tgaagaagcc
     9781 cagaaggcga tcgagatgtg gaacggccag gacttcgacg gaaggaaact taccgtcaac
     9841 gaagcgaagc cgcttgaagc ccgcgccccg cacaccggcg gcggaaggga cggcggagac
     9901 cgttttcaga gaaggcagag ctggtaattc attttaccgc ttataaaaat cccccggctt
     9961 cggccggggg atttttaatt ttacccgata tccggcccga tactccggcg gaatatttta
    10021 tcgttccatc ccgaaacccg aaccaaaaaa atttaagaaa aaattcacgg atcgcgcgcg
    10081 cggcggaaac ggaacaatga acaccgcgga ttccatgcat tgaaatacct cgctacccat
    10141 tccgtcgtcc tcgcggagac tcgccctatg gagtgacgac ttcgcctgct ccatagggcg
    10201 aggatccatg ctttaagaat ttaagacggg aagcgtggat cctcctcttc aggaggacga
    10261 cagaggggaa aatgcctcga taaatcggta cttgatcata agctcataag cagtgagagg
    10321 ttattatttc atgatttcat tgaacttttc gctccaattc ggtactatat tcctattgcc
    10381 gacgtagctc agtggcagag cagggctttc ataaggccaa ggtcgcaggt tcgatccctg
    10441 ccgtcggcac gtcagaagtt cgttggtccg cttggcggca tcacggcttt ttgcgccacg
    10501 tcacaggtcg cacaagcaaa ggagaaataa aaaggccatt ccattatatt aggacttgcg
    10561 catttgaaaa aacacaagaa aatgcctcgc actcggcaca agcgcgtaaa tcctataaga
    10621 ttaagattat gagtaagctt attctcaaac aaaacccgac gctccgcgat tttcaggatt
    10681 atgtcgcccg catgatcaaa aaacgcggct tcgacaagaa aaccgttccg gaattattca
    10741 tgctttttct cgaggaatgc ggagagatgg cgaaagccgc gcgcaaaatg cgaaagatga
    10801 aaacagacac gaactccgag cggttcattt tcgaccatga ggccgccgat gtttttatct
    10861 atctgctgga tatctgcaac cattacggcg tcgatcttga aaaagcgttc cgcgacaagg
    10921 aggcaatcaa taaaaaacgg acgtgggaat aacggcggcg cgggacacaa aacacggcgg
    10981 gacggaaaat aatcgcatga aaatttttgt aaaagcaaaa ccttcttcga aaaaagagac
    11041 ggtgaccccc attgacgatt cccatttcat aatcgcggtc aaagcgccgg cgcgggaagg
    11101 caaggccacc atcgcgatcg ccgaagcgct cgccgcgcat tgcaatgtcc ccttttcccg
    11161 cgtccgcctc gtctccggat tctcaagcaa ggaaaaagtc ttcgagatcg tataatagcg
    11221 cgtccgccgc agaaacaata ttctctcgcc aagccagaga ccatactttc gccgatctgg
    11281 gcttcgccca aagacgataa gcacggaata ctgataaggt gctcgcctcg cgccgtccat
    11341 gcacggggca agcagtaact gaactcctat aaatacactt ttatggctca tcgccactat
    11401 ttacgaaatc attatcaaat ggttctaatc gcctcataat tgatacctcg tatatccaat
    11461 cgatgtaatc gagcgccttc gtttcaactt tgtcgcctct cacatggtct acgtctattg
    11521 tctttccgac aaaagctttc ttgagaacaa tttttagggc gtctaaaaaa tcctgagtca
    11581 aaccgcaatg tctcttaagc gcctcgacag ttagattctt agtgtaactt gttggacttt
    11641 ctttaaagcc ttccggcgaa acattaatca tccacgatat ttcatcaatg tgcactactc
    11701 tgtttctcat attgatcaag tccttcagtt tgtcaataag tttactgtac tcttcaaatg
    11761 gtaattggcc gcagaatgtc ttaaatagga attccaattt actcttcaaa tttttcggtt
    11821 gttctttata ttttccgtac tctctattac cagcaattgg atcaggaata cagaggagat
    11881 tttctataat cttatcgata gtggcctcca cgaaaagata caaaaacaag tacgccagcg
    11941 ctatttcact ggcgtatgtc cgctcagtgc gctcgctgat ttctttctgg tgaatgatat
    12001 taatctgcac ttgaagtttt ctaaaccaga ggtcaattgt tgaattagta actttaccca
    12061 taggtttttt ataacaagct cttcgtaaaa ctggagcggg ataccagaat cggactggcg
    12121 cctcaacctt gggaaggtcg cgtactacca ttatactaat cccgcattat taatgtatcg
    12181 cgcaggttgg cttggaagat tgctctgcca ctgaaccata cccgcattat tttcctaatc
    12241 gctgaccgcg gtcgaactct gatcgggaac gataatgatc atcgtttcgc cgggcttttt
    12301 gtaattgaac cgtgaacgaa gttccttttc cagattcaac ggattcgcaa gatactggac
    12361 gtcggattgc aagttcgcca catcgacctg cgccgacgta aggcgcgcct tgatgcccgc
    12421 aaggctctgg cttaactgtt gctcctgtcc ccaaaaagaa tacgctttca taccggcaaa
    12481 gacgaggatg acagccaaaa taatagcggc ggcgattttc atcgtcatca ttatcgcaca
    12541 gaatttattt tttgacatcc gctatcgccg caccgtcgga tagcgtcccc gcgaaggcgg
    12601 agatccatgc tcccgatttt ttctttattc atttctaaag cgcggatccc cgaccggagt
    12661 cgaggaagac ggaaaagacg ttttgacgga ctgcgaataa ttttaaacac cgtcactttt
    12721 tcaatatctc gcggaacact tcgggcgaga gctcgacgcg cgcggtgcct tcaagccgtt
    12781 ttttgccgcg ctgctgtttt ttccagagtt tcattttgcg cgtccggtcg ccgccgttct
    12841 ttccgaaatt accgagttct tttttcatcg cgggaatcgt ctcgcgcgca attatttttc
    12901 ccagcgcgct cgcctgcagc gcctgcgaaa attgttcctg cggcaaaagc gtcttcagcc
    12961 gttcgacggt ctgccgcccc tcgcgctcaa tatctttctt tggcacgatg cgggtaagcg
    13021 ccggtacaat ctcgcccacg acgacaatct ccaatttttc aacctccgcc tcccgctccc
    13081 ccgccagttc gtacgtaaaa gaagcgtagc cctgcgaaac cgatttcagg cggtcgtcga
    13141 aatcgcggat gagctcgcgc aacggcatca acgcttcaat caaaagatta tcgccgatgt
    13201 tttttatttc cgctatctcg atgtcgaata ccgtctgcaa gcccacgacc tggctcagat
    13261 actgctgcgg cgcaaggatc tcgagcgcga cgatcggctc ccatactttt tccggcttgt
    13321 ccggaaaatc ctgcggctgt tttattattg tttcctgccc gttgtttctc acgcggtatg
    13381 cgatggaagg aaaacttgtc agaaattcaa gattgtattc gcgcgcaagc cggtcggacg
    13441 tgatttcaaa atgaagctgt cccaaaaaac ccactttcaa accgcgcccc aacgcctcgt
    13501 tcgcatccgg ttccaggttt aatgccgcat cgttcaaccg caatttttca aacgcctttt
    13561 tcaagtcgtc gaatttcgtc gacccgtcgg ggtaaaacga aatgaaaacg accggcgtcg
    13621 gatctttata gccggcaagc gcttcctttt tttcctgccg cgcatcaatc gattcgcgat
    13681 gggcgatgat ggtatcccct atcttcacga gcgccggctc tttcaatccc gtcgcgatat
    13741 agcctatctc gccttcggca atcgcctgcg acgcgataag attcggcgaa aaaaacccca
    13801 cttccttcgc cttgaacaca gcgccgcttg cgataagctt catgtcttcc gctttgaagc
    13861 ctccctccgc agttttttca ggcaaaattc gggacggcaa acttccaaaa acgcgcacga
    13921 acgcgataat gcccttgtgg tcgtcgtaca gcgaatcgaa aataagcgcg cgggataatg
    13981 cggcatgcgg caatgcaata tcttttccgt ccgccatgcc gccgttcacg cttatttttt
    14041 tcggcggcgg cactttttca acgacggccc gaagcagttc cgcgacgccg gcgcccgtct
    14101 ttgcggaaac ccgaaaaacg ctttcttcgg aaacgccgag caatgcggcg gtcgagcgga
    14161 caacattgtc tatctgcggc gcggcaaggt cgatcttatt gaccgcggga ataatagtaa
    14221 gccccgcttt ttgcgcggca cggagattgg aaagcgtctg cgcctggatg cctttcgttc
    14281 cgtcgataag caggatcgct ccttcaaccg ccatcagcgc gcgcgaaacc tcgtaagaaa
    14341 aatccgaatg gccgggcgta tcgataagat tgaggatata cgaccgcccg tccatctcat
    14401 agcgcatccg caccggcgcc atttttatgg taatgccgcg ctcgcgttcc aaaggaagcc
    14461 ggtcgagata ttgcggctgc atggaacgca tcggcaccgt tcccgtaacc tccagaagcc
    14521 ggtcggcaag cgtcgacttc ccggaatcga tatgcgcgat aatgacgaaa tttctgatgg
    14581 tattcatgat attcatatag taaactatcc cgggaatatc cgcgaaaaaa ataaaaaaaa
    14641 tctgttcttt ctcgtcgttc ccgcccttaa ccgggtcgag gggcatacgc cggcttgccc
    14701 gcctaagttt cggaaaaaaa cttgggcggg cggggatcca tattccggaa aattaagacg
    14761 ggaacccttc ctcgcctccc ctgtccgaag agaaaaaacc agaagcatgg gtcgtctctc
    14821 ttcagggacg atggcaggag gagtcagatg acgatagaaa agattgccat catccgtttt
    14881 ctttctttct ctctgtcatc gtccggcttg gccggacaat ccacgcttcc cgtctttttc
    14941 ttttctttta aaatccgcgc ctcgatagcc gaaaacgatt ttcgggacga cccccgaagt
    15001 gtcgtcaatt tttcaggtac ttccggaccg cgataaaact cgagctcgcg ccgatcacga
    15061 cgccgaaacc aagttcataa aggaacagtt ggtaaagatg ggtataaaaa tattggaaga
    15121 gatcgaggcc cggaatgaaa acattcaaat acggcgaaac accataaacg atcggcacca
    15181 taatgatgat acttgcaacc gccgcgatca tgccggcgat catgccggcg atcacgtagg
    15241 ggccgcgcac cagcccgttt gaagccccga ccacgcgcat cacgccgatc tcgtcgcggt
    15301 tcgaatatat ggcaaggcgg atggtattga aaacaacaag tccggcgata agcgtaagga
    15361 aaatgatgat aatgatggcg ccgctctcga cgtctttcac aatcgtcgca aggcggttga
    15421 tgacatcctg gttctgcgta tagctcaccg tatcgatata ttgcgcaaga tcgggcgatg
    15481 cgaggtagct tgcgatagcg gcatactggt ctggcttttt cgccttgata ttgagcgacg
    15541 cctcgagcgg attcgtgttg agctcgttga tcgcctgcgt aatcgtcgga tcgttcgcat
    15601 ggttcgccgt aaaattagca agggcgttgt cgcgcgaagt gtaatcgacc gatgccacgt
    15661 ccggcattcc ctgcaacgac tgctggatat tcagtatctc gtcttcggat gtgttcgtgt
    15721 tgaaatagac gctgatgtcg atcttatctt cgatggaggc cacggcgcgg ttcgcaacca
    15781 cgccgaacat gatcaaccct ccaaaaacaa ggagggcgag cgtcataatg gcgaccgtcg
    15841 tcgtggaaag ccatccgttc cggcggaagt tatcgaaacc gaaatgaaaa atgcgcgcaa
    15901 tgatggtgaa catgttttaa ttagaattta gaaattagaa tacggaattt agcataaaaa
    15961 atgaatagca gaatttggaa tttagatgat tttttgtctt ctgttttcta tattctaaat
    16021 tctggattct atattctatt tatatcagga tatatttccc ttttggatcg tcgcggacaa
    16081 tccttccctt gtccatggtg atcacccttt tctccagatt attgacgacc tccttgtcgt
    16141 gcgtcgccaa aagcaccgtc gtgccgagtt cgttgatttt ttccagaagc ttcacgatct
    16201 caagcgtatg gaacgggtca agattgccgg tcggctcatc ggcgataata acttccggcc
    16261 ggtggatcat ggcgcgggcg atcgcgaccc gctgcagctc gccgcccgaa agttccttcg
    16321 gaaaattctt catcttgtcg cccagcccca ccatgtccaa aacctgcggc accagctctt
    16381 ttatctcgcg cgaacggcgg ccggcgacct caagcgcgaa cgcgacgttt tcataagcgg
    16441 ttttcgtcgg cagaagccgg aaatcctgga aaacaacgcc gatgtgccgc cggaacgcgg
    16501 gaagatcctc gtccttcaat ttattcacct cgtattggcc gaaaaacacg cgccccttgg
    16561 acggtttgtc ttcgccgatc agaagcttga taatggtcga ttttcccgca cccgaccgcc
    16621 ccgcgagcga cacgaactcc cccacgccta ccttgaaatc gacttcctca agcgccggtg
    16681 ccttgccgtt atagtttttt gaaactccct gaaaaacaat cattattatt ttatagagga
    16741 tgcttgaaaa ccgcacattt ctgcaatgct tctcttcctt gtcagaataa accattaccg
    16801 caaaaaactc aacacctgcg ttgcagcatg gaaatatttc catttattca aaaccaaaca
    16861 ccctgccgca aagcggacgg ggtgttttaa agaaaaatct ttctagtctt tccgcgcgat
    16921 cacggagcta cttcctgcgt ggtcggattt gcaacgataa cggtctggtt tcctgtgatc
    16981 gaaccgccag gcccggagca ggagaggatg tacttagtcg tcgcagaagg atagatggga
    17041 taagaggatt cattagtgcc gacggaaatt tcattcaaag tgcatgacgt cacatttgaa
    17101 gtggaccact gaagcgtcgc atgttgcggt aagacgattg tcgggttgct catatcatcc
    17161 gccagaaaag tgtttatcac cggcgtggcg gcaacgcagg atgttccaga aagagtgtaa
    17221 cctaagttgc aagtgatggc gcaggaagga gacggactga ccgttccgtt ggtgacggag
    17281 gaagggctgc agacgggttg tgtagaagca ggttgtgtag aagcaggttg tgtagaagca
    17341 ggttgtgtag aagcagcgac cgcataagga atattcaggc ctccggtaac gctgaattga
    17401 acagaactat tctggtacga agcgccatga ttacaagtgc cattattaca agcagcaaca
    17461 taatatagat actcagtact agtagggaca agaatggtat gcgcaattct tgcagtacga
    17521 tcataataag cgtaacatct tgtctgacca tgacgattcc tatagcaact ccttatctct
    17581 cctccactag aataagtagt caaaatatga gcaggaataa tgtgagcgac acgagcgcct
    17641 gtggatatcg atacgcttaa atgaacagta taattgccgg gcgttgacgg tgccgcaaac
    17701 gagagagatg aaaaagacgc agaattttcg ccatagtaat cggaactatt caagaaatac
    17761 cccgtggatc cgtttgcggt ggcactgtaa gaaatactgt aacccgaatt agcacaccaa
    17821 gatactgtcg cgctgccggt aacggtgatg ggcgatccgg gatcataaga ggacttatct
    17881 aaatttgcgg taaaataatt ctgtacgtta tcgccactgt tgctgttgat cgacgacggc
    17941 gaagataccg caaacgtatc cgctttcgcc ggtctcccga acattataac gccgcccgcg
    18001 ataagaaaga acatgatcat catcgttccc gcggaaccca ttgcgccttt ttttcttctc
    18061 aaaagtagga aaagagcgag tgccgcgagg aggatgatta gaacgatgaa cccgatggtc
    18121 gtccatacgg aagcggcatt ggcaggtttc gttgcaacgg aaaaatcgtt ctgtgcgagt
    18181 atcgtcccgc ttgcgttcat gatcgcgaca accgccgtcg gattttgaca gttattcgtg
    18241 atggcgaccg aaatattctc caatccgttc tgctccgaaa gcggcatcgc atagggcgcg
    18301 gcgcacggat tgccgttgcc atcgttcacc tgaagcgcaa gcgtcgcggt cgtcgtcgtc
    18361 attcccaagc gggaacccgg gaacgaatcg gccgaaccgg accagaggaa cgatattttt
    18421 gccgtgtcgc ctttctggta cgaatcctta tcgaaaacga cgttctggat ggtcgcgctc
    18481 tccccgtgca gcacataatg gaacgtcacg ctgttcgaga tggcgtttcc cttgccgtca
    18541 aggaatgtca agattgcgtc ataagcctgc ggaaccgttg ctttaggaat cacgaaagac
    18601 gcggcggtag tggtggcggc gaaaaaggct atgggatcct gattttgagt gccgacaacg
    18661 gcgccgaatg cgtcgcggta atgcgtaacg aaagaaggaa cggcgcttgt gttgctctga
    18721 aacgagctct gcacatcgca gtgagcaatg agatcttccg acggattgac atcaacgccc
    18781 tgagtaagcg tatagcgcgt attcgaggcg tcatcgctga ccgtcagata acacgagtcg
    18841 gaaacgatag cgagagactg tcctgttccc tggaatgtta ctgttccggc gggcgtcgaa
    18901 ccgaaaggaa aaccgtcttc gttttttgcc tctacgacaa tcagatactg gcccgagaga
    18961 aagctcggcg catcataggt gacggtgata ttcttttggt cgttcgccgt aagggaaacg
    19021 acgtcgctat atatcttttc gtcggcgagg atctgggtgc tcgagttcac ctgctgtatc
    19081 accattatgc cgtactttat ctgtggctgc gctcccacgc ggttcgtaag atcgaaattg
    19141 acggtaatct ggtttccggt ctgacttacg atcttcgcgt tctgaacatt gaccgtggca
    19201 acggtcacaa cctgcgccgg cgtagaagtt gccgtcgtcg tagcagcgaa actcacgcct
    19261 gcggaaagga acaatgacgc gaatactatc gacaaaatta ttttatttct catattttta
    19321 tttatatatt ttattgaacc gagccaaaaa aacgcgactt taaatttata gtaaaagtat
    19381 accatatcaa aaagcacggc ctgtgcataa cttttagaac ggaaccgcgc aagaaaataa
    19441 aaaatcgttt ttgccgcatc aagaaaaata aaaaaacccc gattgctcgg gggtttttgc
    19501 cggtaatccg taagccggat tctgtgcctc cgatttgcat cagagcgacg atcatttgtc
    19561 ttggccatcg attgctcttt ggctcatgcg gcaccccatc gttgcccggc aacgatgata
    19621 cggccttgca cccggtaagg attttgccgt ttcacctccg cttttttgtc cctatggcag
    19681 agttagacca tctacgttgg gataggggtc ctccttcggc tttcgcctcg agacgtcact
    19741 gctcgcacct ctacgcttgc gcgcgacggc tgttagccgc tacctcatgt aactcgaaat
    19801 ttcgagtgtg gtgtccggac tttcctcccc ttcgatttaa tgaaggagta atcgtctgga
    19861 ttgccggctt ttgcatagta ccattttcat gccataaacg caattttttc tttccatagg
    19921 cctcgcggag gctcgccccg cggaatggcg gcttcgccta ctccatagag cgaggatcca
    19981 cgcttttaaa aaaaacggaa agacagggag cagagaccct ccctccagga gaacggcaga
    20041 aaagcgtgtt atcataaaga aatgaaattt tttatctcct tttcccacgc cgtctatcgc
    20101 ggcatatgcc ggccgattat ttttcgtttt gattcggaga acgtccatgt tttttttacg
    20161 aatacgggcg aatggatggg gcgctcgcga ttgatgacgg ggatattcca tgccgcgttc
    20221 cgcgttgaca atcccgcact cgcgcaaacc gtagcgggaa tccgttttga aaatcccgtc
    20281 ggcctcgcgg cgggcttcga ttaccgcgcc cagctgacag acatactccc cgcgatcgga
    20341 ttcggtttcg gcacgatcgg caccatcaca aaccggccct atgaaggcaa tcccgcgcca
    20401 cggctcggcc ggcttataaa atcccattcg ctcatcgtaa ataaagggtt caagaacgac
    20461 ggcatcgatg ccatgctcga aaaatcgaga cgattcttat tgcgtcggcc tcgccctgta
    20521 gcgcggcacg ggataaactc cggcccgccc cgcggaatgg cggcttcgcc tgctccgcag
    20581 ggcgaggatt cacactttaa agaatttgat cggacatttc cgtttccggt cggattaagt
    20641 ttgggaaaaa cgaattcacg cgccgcgatg acgcaggaag aagcggtcga ggatgttgcc
    20701 gccgcgttcg aaaaagccga aacatcgcgg ataccttttt cgtattatga actgaatatc
    20761 agctgtccga atttgtacgg ctcggtaacg ttctatccgc cggaaaatct ccgcgcgctc
    20821 ctctcggcaa ttgctgcggt taatctttcg cggccgcttt tcataaaaat gccgatagaa
    20881 aaaacggatg atgaaacgct cgcgatactc cgcacggccg ccgaatttcc cgttgccggc
    20941 gtcattcttg gaaacctcca aaaaaaccgg catgatcccg cattcgtaaa aaaagaaatc
    21001 gaaaacgccg gcgccggcca cttcagcggc gttccgaccc gccggcgttc cgatgagctt
    21061 atccggctca gctaccgaga gttcggtaaa aaactcgtga ttatcggctg cggagggata
    21121 ttttccgccg aagacgccta ccgcaaaatc cgtctcggcg ccacactcgt gcaatgcatt
    21181 accggcatga tttatgaagg cccccaactc gccggcgccg tgaacgccgg ccttgcggac
    21241 ctgctcaaaa aagacggatt taaaaatatc tctgaagcgg tcggcgtgga tgcataaaaa
    21301 cgcacaaaaa aacagcaata aatctgtcat cgtccgtgaa ggcggacgac ccgcgctttc
    21361 cgtcttaatt tcctaaaaca tgggtcctcg ccctatggag tggcggcttc gcctactcca
    21421 tagggcgagg atgacaatgg agcatacact aaaaaacaac tatctccgct tccgccattc
    21481 ctctattttc ttatgatcgc ccgatagaag cactttcgga actgaccata ctgcggtttt
    21541 ttttgattcc gattttcttt tcctcccgga tttttccggg aaaaattctg gaggccgcgt
    21601 ataagtcggg aacgaaccgt ttttctcctc aagcgattct tctttgccga gaaaccctgg
    21661 cacgaaccgg cttaccgctt ccatgacggc aagcgcggga atttcgccgc cggacagcac
    21721 gtaatccccc atcgaaattt cctcgtcggc aacatgttcc gccacgcgtt cgtcgacgcc
    21781 ttcataacgg ccgcaaatga aaatcagcgc gtcgtacttc gcaagccgcc gcgcgctttc
    21841 cgcgtcgaat ttttttcccc gcgtggaaaa aagaataatc cgcacttttg atttttttaa
    21901 gcgtgaatcc ttgccttcgc gaggacgaca taagaatgtc gcttttgact taatgtcttc
    21961 cactgctcta tagatcggcg gaagctggag caccattccc ggcccgccgc cgaacggccg
    22021 gtcgtccact ttcgcgaacg gttgccgctt gcccgctatt tttttgccgt accgcgcatc
    22081 cgaaaaatcg cggaggtcgt gcgtcgcgac ggaaacaatc ttgtttttca cggcacggcg
    22141 aaacagcgat tcgttggtat acgaatcgaa catgcccggg aaaatggtaa tgatatggaa
    22201 tttcacaaag aaggtttgga actctctgtt ttgtttccgt cgtcctcgtc ccgtgccacg
    22261 atacggaacg gggacgacgg aagagctgga ttattaattt tattcgggct tctttttgag
    22321 cgattcaccg atcttgcggc gcatttcctc gatgcttgcg gacggttttt gatattcttt
    22381 cttcggctcc ggaaccggct gcgacgcgga ttctttattg gtatcgtttg cgcgatcggc
    22441 cggcacatta ttgactgacg tcgacgcaat ggcgcgcggt ttttcccgtt cattccgccg
    22501 cccgccggtt ttcaaaacgt catacagatg cttctcgtcc cgccggccga ttttttcttc
    22561 cagaatcgcg tcgctttttg tttcccattc cgaagcgatc ttcgcgtcaa cgaccgcgcg
    22621 cggcgttccg tattttttct gcgtattttt cacaatgaca tcgcggtaag aaacaagggg
    22681 cttcggctca ggcggaattg tctccgcgga aaaaggactg gatgccacgc cgtcaatcat
    22741 aagctttata taaatattcg ctttccccag gttcacgaga tcgttttcgg taaattccgg
    22801 cataaattct ttttcgagga attccgcgtc ggcgccgccc acgcgaaaag cgataatcgt
    22861 gccgacgttc ccgaacaccg catcgcgcac cttggtgttg tcggcctcca caagctggct
    22921 gatatattga tgcgcaagga tgagccccag ccggtatttc cgcgcttcgg aaagaatgtt
    22981 cgcgaacgat tccgtggcga aattctggaa ctcgtcgacg taaagaaaaa aatcattgcg
    23041 cttgctttcg agaatatcca cgcgcgacat cgccgcgagc tgaagccgcg tgatcatcat
    23101 cgcgcccaga agccgcgaat tatcttcgcc tatcttgcct ttcgaaagat tcacgatgag
    23161 gatttttccg ccgtccatga tggcgcgcat attgatcgtc gagcgcgatt gccccatgat
    23221 attccttatc agcggattgg aaatgaactg tcccacttta ttctggatcg cggcaagcgc
    23281 ttccgtctca agccgctggg tatatttcgc aaactcgttc gcccaaaaag ccttgataac
    23341 gggatcctgc aggttatcca cgatcttctt ccggtacgcc ggttccgcga acatccgcat
    23401 aatgccgagc agcgtcgaat cgggaaattc aagaagcgca agaatggtat tgttcagaat
    23461 atattccatg cgcgccgacc atgcatccgg ccagatcttc ttgaacacgc ccatgatgcc
    23521 cgacgccacg agatgccgga attcgccgtc cacctgttcc atcggattga acgcgatggg
    23581 attgtcggta tccgacggat cgaaatagat cacgtcatcc agccgctctt ccggaatgaa
    23641 atccagtatc ttttccgcgg attccccgtg cggatcgaaa aatgccacgc ctttcccggc
    23701 gcggatatcc gcgatgatca tattttccaa aagcgtcgat ttcccgacgc cggtttttcc
    23761 gactatataa acatgccggc ggcgatcgtc gagcttgatg ccgaattttt tccgctggcg
    23821 cctgaaatta taaaaaccga gcggcgtgat atcgggagca ttgtcggaca tatgcgtatt
    23881 ttaacacaga aaagaaaagt cgtattcttt gacgcctgcg ctatcgacgc cactccgtca
    23941 tcatcacaga ggcgagggcc catgttttag gaaattaaaa cgggggaagc gcgggtcgtc
    24001 cgccttcgcg gacgatggca agaaagaagc caaagaataa gaaaaattat tcttccacca
    24061 tcggcaaact ggacggaggg ccgcctttgc gcgaagaaac cttttccaat tcggtcgtac
    24121 ccacggcggc aatcgggaag tgatatatcg ttgccaactc ctcggtattc aaaatggggg
    24181 gcttgcccgg gctgaactca atatcgcgat acctttcata gaccatccgt ttccgccagt
    24241 tattcctttt ttccttggat aatcccttga cggacggcag cgttttcgaa tccggcttga
    24301 aagaattgag gttctgcgtg ctgaactgtt tgagaaaagc gctgaccgac gaaatattat
    24361 ctcttttaaa cgtttcgcgt ttatcgatat aaataaaccg gatggtcgtt tcaaaagcga
    24421 tcttcgatat tttttcccgg ataccgtcaa tgatgtcttt ggtcgaggga ttcgaaagaa
    24481 gcaatttcat attcatggct ttctcttctt tacgcttcac ttcctgactg ggatgctcga
    24541 aaggcgcggc tatcagctcg tcgagcgtaa aacccagcca ttggaaatcg gcaagccatc
    24601 ctttcttttt ttcttccacg atgccgagaa gcttattgat gattgcctcg ccttcttttg
    24661 cccagtcgcc gcccaccgga cgcacgataa gctgaatcca catctgttcg ttatccttga
    24721 gcttcgccat cgcttccatg atgaacgcaa cggtatcgat gcgctgttcc tcgaccgctt
    24781 cttcgaacat cggataggtg cggataggat aacagttctt attgccgagt atccattcgc
    24841 cgccgaaaat atcgaaatca ttgttcggca ggatattcgg catctgcgcc acgtaattat
    24901 ccgcttcgac gatttcggct tccggatatt gcgcatagat cgccgattcc atcatgggac
    24961 ggtactgttc gggaacgcgc agataaaaat ggctttctcc ggcgcggccg accagctcga
    25021 acgacatgaa atagtccaat tccccttccc agtatttttc gaaaaatcgg tgcctggctt
    25081 tatgcatggc gtgcgcggcg gaaaaaacct gctccatcgc cttcggtgtt ttcagaatgt
    25141 ttttcggaac ctgtatctca agcgttttcc agttgatttt tttcagccac cggatatgga
    25201 tatacagcat ccaaaactcc caaaaaatga ttgccgcgat aacgggaacg gcaatccacc
    25261 agacggcgcc ccaaacaacg gcaatgctgc gcgcgattgc caatgcctgg gagaaaataa
    25321 acgaaaccat ggcatccata tcaaaagtat atcatggagt tgccattgtc cgcatgcgtg
    25381 gatagccaac actcccttat tctaaaaccc gtgccaaaag caggccagcg gggcgagtaa
    25441 tatcctcaat cgcttacgat tcccgcacgc aataggtgcc gtcggaaaga cgttcaaaga
    25501 atttcttgtt ctgaagattg ataagaatcg tattctgccg gaaggaatat ttttgattga
    25561 catgggaaac cacgtcgctc gaatgaaggg gtcctttctt tttcaaaacc ttcgcaattg
    25621 cctggcacgc ggtgcccgtt tcatagccat attcccgcaa gccgtacatg ccgcggccta
    25681 tcagcgcaaa acggtcgtct tttataagct cattgtgcac cgtcggtgtg agcgccttat
    25741 ggtcgtcaaa acgggctttg ttgatatggt cggcgatcgc ttcgaaatgc aacggctctt
    25801 ttttcttttt catgacgaga taaaccttgt cccgtatcgt cgccggattg atttccggcc
    25861 attcgcgaag ccccacatcg ccatatggac ttttcttgat gctctttgaa atggcaaggt
    25921 aactttccgc ggcagtcgaa agcgccggtt tcattttcaa gaaacccgcg agcgtcgcgt
    25981 catacgaacc ggaaaggact ttttccttgc gggaccgcag ataattcgtc caactgtcca
    26041 cgaacgcgaa cgccgccttc atatcttttc tggtaatgta ataaaatggg aaataatcgt
    26101 tatctcctga aaaaagattg aaaaaacccg aggcctcgcc aagaaaatcg atctgattgc
    26161 cggtcgcgtc ggtcaaaaag accgttgcat attggacaac cgcatccttt ttcatcgcgc
    26221 cgccgttttt tgtgatatgt tcgccgatct ttttgagcga agcggcgaca tcgccgtcct
    26281 tcaaaagatt ttccttcgcg agcgatattc ccgcacactc gatctggcgg atccgttcgc
    26341 gcgtgacatc catccgttcg ccgatcgccg caagcgtctc tcctttcccc tctttcttat
    26401 taagaccgaa acgtcccact aaaacatcct tctgccgcgg cgtaaggttc gcaagcgcgg
    26461 tatttattgt ttttgatatc ggcgccataa aggtgtcttt aatatttgaa agtattatat
    26521 actataacga tttattttgc aagtggcgac gtggtaacca ttcactatct atagtcagcg
    26581 ccgattccgt cgaagcattt tcccctccgt cgtcccccga aggaggaaat ccatactccc
    26641 gatccgaaga tttcaaagcg cggatccttg acctgtggag tggcggcgt
//
LOCUS       DAIDDT010000003        24150 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_3_length_24150_cov_6.434055, whole genome shotgun sequence.
ACCESSION   DAIDDT010000003 DAIDDT010000000
VERSION     DAIDDT010000003.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 24150)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24150)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3_length_24150_cov_6.434055"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5695 a         5966 c         6634 g         5855 t
ORIGIN      
        1 atctcgggct cggccagcaa agccttgagc gcgagcggcg gattgcaggc gccaaggatg
       61 atgtagcgct tgaagtccac tccgagcttc tccttcatct tttccttgat gtcgatttcg
      121 gagagaatac ggcgctgccg cctatttatc atgctttaaa aataaaaacg aaagaccgta
      181 ctgttatttt cgaggttatg aagcatttgg agccgggcaa agtgcgcgca atcgcgctcg
      241 attcaaccga cggattgagc cgcggcatgg caatcgaaga tatggggaag atgatcgagg
      301 tgccggtggg agagcgggtg ctggggcata ttttcgatgt cgtcggaaac tgtctggatg
      361 ctccggaaga atttccgtct tcatcaaaaa gcgaacccgt ccacggcgta ccggcttcgg
      421 atggaaaaaa atttttgccg atccaccgtg atgcgccgac ctttaccgag caatccacga
      481 aaaccgaagt tttcgaaacg ggtattaaag tcatcgatct tatctgcccg ttcatcaagg
      541 gcggcaaggt cggtcttttc ggcggcgcgg gcgtggggaa aaccgtgctt ttgcaggagc
      601 tcatccataa cgtcgcggaa gtcggcggcg gcgtttcggt ctttgccggc gtcggcgaac
      661 gttcgcgcga agggaacgat ctttataatg aaatgaagga ttcgggcgtc atcggaaaga
      721 ccgcgctcgt gttcggccag atgaatgaag tgccgggcgc ccgcgcgcgc gtggcgctgt
      781 ccgcgctcac gatggcggaa tatttccgcg acgaggaagg gaagaacacg ctgcttttca
      841 tcgacaatat tttccgcttt tcgcaggcgg gcgccgaagt ttcgacgctt ttgggcagga
      901 tgccttcggc cgtgggatat cagccgactc ttgcgtccga gatggcggag cttcaggagc
      961 ggatcacgtc gaccacgaaa ggttccatca cgtccgtgca ggcggtttat gttccggccg
     1021 acgatatcac ggatcccgcg cccgcgacga cattcagcca ccttgattcc acggttgttc
     1081 tttcgcgcgc attgaccgag atcggcattt atccggcggt ggatcccttg tcgtccaatt
     1141 cgagcgcgct cgatccgaag atcgtcggcc gcgagcatta cgacgtcgcg cgcgaagtgc
     1201 agcagatatt gcagcgccat aaagagcttc aggatatcat cgcgatcctt ggtatcgagg
     1261 aactgtcgga tgccgaccgg aagaccgtcg accgcgcccg caagatccag cgcttccttt
     1321 cccagccgtt tgcggtcgcg gaaccgttca ccggccgtcc cggaaaatat gttccgctca
     1381 aagacactat aaaaagtttc cgcgagattt tggacggcaa atatgacgac aagccggaag
     1441 aggatttcta tctgaagggc gggatagagg aagtggggag ctgattcctg cattctctgt
     1501 cgtcctcgcc ctatggagtg gcggcttcgc ctactccata gggcgaggat ctacgcttga
     1561 aacgtataat acaaaaagca gaatggagaa agagaagatg gaagcatgga tcctcgaccg
     1621 gggcgaggac gacggaaaaa gaattttaat ttttaatcgc taataacttt tcacttttca
     1681 ctattaactt cttcgcgctt ttcactaata acttttcact tctcatgact ctcggcgttt
     1741 attcattgga aaaagtcctc tatcgcggcg aggcggtttc ggtgaattgc gcgaccgcga
     1801 tgggggaaat cacgatcctc gaccatcacc agcaaatgat ttccatttta aaaaaaggcg
     1861 tgatgaaaat agttgacaat aatggtaaag agcattatat taatgtcagc tctggtttcc
     1921 tggaagtggg ttccgggaac gaaattaaaa ttttaataga agaggtattg taaggagggc
     1981 accgtgaaga aagaatatga aggccgttta aaattgtcga ttgaagaccg caatctagta
     2041 aggaaacttc gaatggacgg tgttttggat gatgcaaaag gacattttct cgaccagaat
     2101 aatggaatta tcctggtaac gtgctccgac ggggatcaat tcttcgatat ttttgaacac
     2161 caggcccaga tgcaggcggg ccaacagcct gccgatccgc gcatccatac ttttggatgg
     2221 catggcgggg cactcgcctg tgcgccctgc tcgccgataa acgagagaaa aaaagccgat
     2281 ctggttttcc tcgaccagat caaagccgct cgtaaaatga aaaacatcga catggtggcg
     2341 ctttatacgc acgcaccctg cggtgcggca agcgggaacg gtgtgaatct cgtaaaggcc
     2401 tttgccctgc aagccggagc aaaggtaaaa ataaaaacct tgaacagggg catcaaggta
     2461 gcttgttttt tccatgttga ttacgggaat ggcgtaaaga gaacgtattt tttttcgcgc
     2521 agaaagtggg atgcatgggc gcgcgagaac gcgaaggaag agcgataacg gctgttatcg
     2581 tcgcagaggt atgaagtgat gcttgatgct atctaagata ataatttcgt cattttcggg
     2641 ccgcacattt tccgtgcggc cctttttatt tttattttcg cgccgctgtc gaaaataagc
     2701 aggatttttg ccgtttcccc tcgcggagga ggatccgcac ttccggtctt ttcctttgaa
     2761 ttttcaaatc gcggatattc agtaaggccg atgatggcgg gaatggaaat atggatttat
     2821 gtttgcgtga agacgacgga acggaaaccg gacgacgccg gattacaaat cgttgcccgt
     2881 atacgaaaga ttgaagctct tgttgatgag gggaaaatcg ggaaccatat tgccctggcc
     2941 cgcataaccg aggatggtcc agttcaaaaa agaaggatcg cggacatcca cgccgcttat
     3001 ttcgccgcgc gggccggtcg ttatgaaata gacgatatcg ccgcgccatc cttcaacgat
     3061 gccgaccgca gtagcgtctt ttttgagatt gattttatcg attgccgccg cggcagtccc
     3121 gtcgggaatt tttttcaaag cttccgtgat aagacggtgg gatgcgtacg cttcttcaat
     3181 gcggacatgg aaacgcgcgt acacgtcgcc gctttgatag aggggaattt tcggtataag
     3241 ccggtcatag gcggcatatg gataatcgat ccgcgcgtcc ttgttgatgc cgacggcttt
     3301 tcccgcgacg ccgataacgc cgtggttttt cgcctcatcg tacggcgtca cgccggtttc
     3361 tttcaggcgg ttgataagcg aagaactttg ctccgcgacc gcaatcactt ccgaaaaatc
     3421 ttttatgata gcgtcaagtt cgccgatgag tcgctgtttc gttcccattg cgatgtcttt
     3481 ggtaacgccg ccgatcgtat tgacgcctct caaaaaacgg cttccggtcg tccgttcgtt
     3541 gatctgcatg atcatttccc gcagccgcgc cccgtgcgct ccgccgaaat tgaatccggt
     3601 atcaagcatg atggcgccga tgtcgccgaa atgattcgcc aaccgttcca gttcggcata
     3661 gaccgtcctc aaatagcgcg cccgttcagg gacgtcgatg cccgccaatc gttcgatggc
     3721 ctgcgagaac gcgagcgaat ggctgaacga gctgtctccg gaaatttttt ccgacaagcg
     3781 gagttttccc tcaagcggga gaatcccgaa taattttacg ctgcctttat gggtatatcc
     3841 gagccgcggt tcgagcagga cgatttcttc tcccgcgacg ctgaaccgga aatgccccgg
     3901 ttcgatgata ccggcatgga taggccctac cgaaatctca tagatgcctt ctcccgcgac
     3961 tttttggaaa tcgtatcgtt cgcgcgcgat cgccggtctt gtttgccagt tgaaatcatt
     4021 gcgcaaagga aaaacgccgc gcggccaatt ttcatgcagg atcatggctt cttttttcgg
     4081 atgtcccgcg ggatccagtc cgaaaaaagt ttttattttt cgttcgtagt tccacgcttc
     4141 gtggatgaac ggggagaccg acgggaactc atccgcattt tttacgcata tgaacggaac
     4201 aaggaaaata ttttctttcg gcgcgccgaa gacataccag attttaaaat aaccgctttc
     4261 atttctttcg tccgtcgcgg tgatgatttt gagcgacaat gcttcgtccg cgtacatctc
     4321 ttttatcgtc gcgacgatgg tatcgatagg cacttcaaaa gtagccgtat tttcggacgg
     4381 gtcaaaatgg aaattttcgg gaagatattt tttcagtttt tgggggatgg cgtgcgtcat
     4441 agcggttatt aataggctgc ggcggcatta tgcagcaagg tgataaggaa cggcggcaga
     4501 gagaaactaa agcagatgaa aataatgacg agcgcgagcg gcggaatgag gatccaataa
     4561 ttccgttctc cggaaccgga accgacgtca tccggccgtt cgccgaacat catcgatgac
     4621 gtatgtttca ggaatccggc gaagaccagc gcgaacgcaa ggattgcgac ggcggtaatc
     4681 acgggatgca tgccaatgcc gccggccaaa atggaaaact cggtcaaaaa tatgccggac
     4741 ggcggcacgc cggtgatggc caggaatccg acgatgaaaa gtatgcccgt tatcggcagc
     4801 gccgcaagcg ccccccgtac ctgttttatt ttggtcgtgc tgtatttcaa aaagatattt
     4861 ccggaggaaa ggaacagcgc cggtttcacg agggcgtgat agaccatatg aagaacggcg
     4921 gcaagcgcgg ccagtccgcc gaatccgaag ccgagcgcga caattcccat attttcgatg
     4981 ctggaatatg ccagaagccg tttgtaattt ttttgcgtca agatggtgaa tgcggccaaa
     5041 ataatcgaag caagcccgaa ccagatcaga agcgtctggg aaaagtgtac gccgaggacg
     5101 ctgtccgtga ctgccttgaa gcggatgatc gcgaggaatg ccacgttgag caggacgccc
     5161 gaaagcaaag cgctgatcgg aaccggcgct ttgctgtgcg cgtcgggaag ccatgtgtgc
     5221 atgggcacca ggccggcttt cgtgccgtat cctataagga cgaatataaa agcgattttc
     5281 gcgacggcgg gattaagatg cccggcattc gcaaaaagca ttccccagct gacgagtccg
     5341 gttccgtgga tggacgtgaa gtagagtagc gttccgaaaa aaccgagcaa caaaccgacc
     5401 gaattgacca tcagatattt ccatgccgct tccatcgccg acggcttatt gtaaaaacta
     5461 atcaggaatg ccgtcgagag cgtcgttgcc tctatcgaaa tccacatgat gatcggattg
     5521 accgtggcga cggcaaagaa catcgccaaa aggaacaggt tgaacaaaat aaaatattgt
     5581 ttgactctgc ggaagccgat gatatttttt gcggtctctt cccggagata tcctttggaa
     5641 tagaacgccg tggcgactcc gaccgcggcg acggcgagca tgatgatcgc gcccagcgcg
     5701 tcgacggaaa aaagcggcaa aaaactgtac gttccgaaag aagcgacctc tatcgcgatt
     5761 gcgaccgccg ccccgaacga gcaaagcgaa gcggcgatcg aaacggtttc gattgttttt
     5821 ctttgtctgc ttgcgatgcc cgtaagcgcc gcaacgaaca atgacgcgat gataatgacg
     5881 atggccatgt gattattctt cttttaaatg tttcatcacg gtggtttcga acgagccgaa
     5941 atggcggtag atcatcgacg cgaatatgga tgcgatggtc acccagatga caagattgaa
     6001 cataatgccc gcctgaagcg ccggcgattg ttccaatccc gagagaacgg aaaacgccac
     6061 aatcccgttt tcaagagaaa gaatgccgac catctgggat aagatgccct tgcggttgat
     6121 gataagcagg aacgctatca gcatgacggc gatggcgagt gttatcatcg gcgcatggtc
     6181 cggcatgatg gcgaccagcg gcgcgaatat ccgcgaatgc gcgagcgccg ccagaagcgc
     6241 aacggccaaa aggctgagcg gcacgttcaa ataagtgctg gcggagaagg tgaggcggtg
     6301 cttttttatc aatcgcgtga agaacaaagg agcgaagacg actttgacgg caaaggtcag
     6361 aagcgcgatc atggcaagcg acacggcgtg cgtttcgaaa aaagaaccga ataggatcag
     6421 ggttattgcc gcggattgta tggcgtaaag cgcgatgacc attgagtttt ttctcgtcac
     6481 gtgcaaaaaa acaaccgtga gaaaaacgat gattacgaag aatgataaaa agttatatgc
     6541 catgattaca ttattaccat gccgattgcg ataatgccca ggataaacga agtaagaagg
     6601 aggtcgggaa gccggaaaaa tctgaacttg gcgatgctcg attcaagcgc ggctattgcg
     6661 gcgcaaaaaa caaggatttt cagcgcaaaa attcccagtc cgaaagctat ggcgcccatg
     6721 cttggcgaaa ttgcgatgcc ccacgggaag aacaggctcg atccgagcgc gatgaagatc
     6781 gcgagcttgt tggccgacgc ccattccatc agcgcgagct tcttccctga atattcaagg
     6841 atcatcgctt cgtggatcat cgtgagttcg agatgggttg ccggattatc gaaaggaaac
     6901 cgcgccgttt ccgcaagaag gacaatcagg aatcccgtga aagcgatgaa tatcggaagg
     6961 aaaaaagaat gtccgtttaa ggatatcgcc gcggcgatgg aaaataagtt cgttgttttg
     7021 gcgatgacgg cgagcgcgaa cagcgaaaag atcaatccgc cttccgcgag cgccgcaacg
     7081 gtcatctcgc ggctcgaacc gaagccgccg aaagagcttc cggcatccat gcctgcgagc
     7141 gccagaaaga acgtgccgat tgcgaggagg tagacgagcg ccagaagatc gccggtgaac
     7201 ttattggaca ggaacgcggc gaatgtcggg atgcccgccg cggcggcgag cgttaccgca
     7261 aaaacgatat acggcgcggc gcggaacagc cacgatgcgt cggtgctgat aatttcgtcc
     7321 ttgtgaagaa gtttccagat atctttatac ggctggaaaa ttcccgctcc tgcgcggttt
     7381 tgaagcaccg ctttcatttt cctgatgatg ccgagcatga gcggcgcgat catcggtaaa
     7441 agaaataatt gcaatgtcca aagcaggatg gtgatcatgg cttaaaataa agatgccgat
     7501 aataacgccg cgagcaggac gatcagcatg tagagaatat acgcattgat gttgccgctt
     7561 tgtatctttt ttacgtatcc ggatattttc atgaaaccga accggatcgg atcgtagaat
     7621 cgcacccggt aaaaatcggc aagatgcatg gtcgccgttc ttgatttcgg aacatagcgc
     7681 atcgaggcgt cgttgtattc tatgtcggtc tggaaactcg gtttcaatat tccctggaag
     7741 atcataataa tggatcgcga gaacccggtc gccgttattt ccatgcgggg cgtgagatcg
     7801 gtgccgcaat cccacgtggc gccggttttg atattttgtt tccggttgac gagatatttg
     7861 atcgcgacta cggaaagggc gagtgccaga atgatgatgg cgaacagcgc cggcgcggaa
     7921 accgaagcaa agccgttcac cgcgattgtc tgggcgggat gaaccgcaac aatcgatgcc
     7981 gcgtcgcgca agccggaaat attttttacg acggcattca ggatggaaac gatggaaccg
     8041 gaaaacaggc cgaatacgag cgacaggagc gcgagtcccg ccatgccaaa ctgcaaagaa
     8101 aatcccgatt ccttcgcgca tgcggcttca tggcttcggg gacgggcgag gaatgtcgcg
     8161 ccgaacgctt ttacgaaaca ggcgagcgca aggccgcttg taagggcgag cgttccggcc
     8221 gacaggatga agatccagac gaccagggaa gagaacgaat aaatacccgc gaagagcgac
     8281 tggaatgtca gccactcgct gaaaaaaccg ttaaaaggag ggagcgcggc gatggccatc
     8341 gatcctatga gaaagaaaaa ggcggtttcg ggcatccgtt tcatgagccc gccgtattct
     8401 tcgatatttc ttgtgcgcgt tccctcgata acggagccgg cgctcaaaaa taacaatgat
     8461 ttaaagacgg cgtggttcaa agtgtggaaa agcgcggcga tcatgcccaa tgccgcgagt
     8521 gccggcatcc cgagcgacga gaaaacaagc gcgctgccga tgccgagcag gatgatgccg
     8581 atattttcga tgctgtggta tgccagaagg cgtttgatgt cgtgttcggt cagcgcataa
     8641 agcacgccca gcaaggcgga tacggatccg atgacgagta tcgcgacgcc ccaccataac
     8701 ggcgccgtcg taaaaacatc gaaaaataat ttgatcatca tgaatatccc cgttttgatc
     8761 atgacgccgg acatcaatgc ggatacgtgc gaaggggcgg cgggatgggc gtcgggaagc
     8821 cagatgtgca aagggatgat gcccgctttc atgccgaaac cgatgaatga aaaaataaag
     8881 atcatgtttt tcgcgaacat gggtatggcg gcgctgttcg ctttgataac gccgaagtcg
     8941 aacgatccgg tgtaccggta taaaagaagg aatgcgagca tgatgaaagc ggtgccgatg
     9001 tgcgtcatga tgaaatagag ggatccggct ttgatatttt tcgggtcgtt ccggtcgtag
     9061 atcaccaaaa aatacgatgc gagcgacatg atttcccaga cgataagaaa caagattgcg
     9121 ttggatgccg taaccaccat cagcatgccg gcgatgaaga aatggtaaaa aaatcccagg
     9181 gtgccgatgg tgtatttttt ataataatgt ttgacgtatc ctatgccgta gatcgaagcg
     9241 aaaagcgcca ccaatgaaat gataaaaata aaaaacgcgg acagcgcgcc gacggcgaac
     9301 gatatcgata aaaaaggaga gagcgccgcg gcgatatgga atgataatgt cgttccggaa
     9361 atgaatatgg aaagcgcgaa aatgatgccc cagagcgacc cgccgacggc gaacgcgctg
     9421 ctccaaaaat ttgcaagcgc gtcatttttc ctgaacgcca atgcgcccaa ggctcccgcg
     9481 gcgaagaatg aaagcagcaa taaaaaaccg ccttgcgatg caatggatgc gatcatgata
     9541 gtgaggctga ttttttatgc tcgatatcgt tgaggatcgc caaaaggtgg cggaggatga
     9601 ttttggggga aggaggatcg cccgggatct gataatcgac gggaatcacg ttatgcgcgc
     9661 cgtccaaaac cgcatatgat cctctataaa taccgccgtc gattgcgtca tcgccgaccg
     9721 ccacgacgat cttcggatcg ggcgtcgccc ggtatgcgcg gaccagcgct tcgcgcatat
     9781 tccttgtcac ggggccggtc accataagca tgtcggcgtg tcttggtgaa gcgacaaaat
     9841 ggatgccgaa gcgttcaatg tcgtaaaaag cgttcgaaag cgcgaccagt tcctgttcgc
     9901 aggcgttatc ggatccggcg tcgacctgac gtatcgcgag cgacccgcgg aataatttct
     9961 tgacgcggtc gcttattttt cctccgagag cggcgacctc gtctttttcc atagcgctgt
    10021 cacggaacgg gatgacgggg cttttcgtcc ggaatatttt tgtgagtagc ctcaacatgg
    10081 attgaatgcg gattgaatta tgataatgcc gcacattata atttgcaaga aaaaaataac
    10141 gattttttta aagcgtggat cctcgccctg tggagtaggc gaagtcgcca ctccataggg
    10201 cgagtctccg cgaggacgac aagaaaaggc aatgacccgc gcttttaaat gaccgcgttc
    10261 ggcggcgccg aaaaacggga agggccatga gggcgttttt aaacaacctc gttttctttc
    10321 agtgtgaacc gatagggttt ttccgcccat gtgcggccgg cgaaattaac gccgattctt
    10381 tttcgcgcgg caatggtccg tttttttatt tttattccgc gatcttcgaa ccataagccg
    10441 gtttttttgc aggcgcgttt tccgttttgc gcatcggcga tatgcaggaa ttttgcaagc
    10501 cgcgccggac cattgacaat aattccctcg ccggttttcg ggtcattata ttttccggcg
    10561 cgcaaaagcg ccgccgcggg ataatccgcg gggccggtca cgatattaac gagccaatgc
    10621 atgccgtata cgaaataaat atagaaatgt cccggttcgc cgaacattat ttccgtccgt
    10681 tccgttctgc cgcggctcgc gtgcgatgcc ttatcgcgcg gtccgtcgta cgcttcgacg
    10741 tcggtgatca tgagcgcgat ccgtttgccg cgccacgatc gcacgatata ttttccgata
    10801 agatctttcg ccgcgacaat cgtcggccga ttgaagaatt tttgcggaag tgttttcgcc
    10861 atgaaatcca atagtataat cattgagagt atatcccaag aggaaatctt tcggtagcgc
    10921 ggttatttcg cggaatgccg ccgattccct gaaacgcgac ccgcttgatt taccccgaaa
    10981 gaaaggatag aattttcgat aaagatgcgt tggtattcat ttatagaacc tgctttaaaa
    11041 gtaagacaga gagtttgctt aaaaagttat ttttgagaca ggttctcatc aatgcggtcg
    11101 tagctcaatt tggttagagc accgccctgt cacggcgggg gttgccggtt caagtccggt
    11161 cgaccgcgcc cctttgggca cgtttgagat accgctgggg ccttcatggt tccagcgggt
    11221 ttgcgacggc cgatgctctt taatctccac cgggcagcct ctaaagcgat aaatactaca
    11281 acttgtttcc ctgccggtcc ttgccgcagg gtccatgcag gaaagtccga aaacctttgt
    11341 tttctgatat accgacctca tgagacgggt tctttttgca acaggaggat caggaagcgc
    11401 cgcctgcaag tcatagactt caaacgcgtc cgcccaagcg cgcataaaaa taaaaccgct
    11461 ctccgatggg cggttttatt tttttctttc ggatatcggt ccggcggcat aacggaattt
    11521 caaaataacg aaacaggatt ctttataaaa tcatcggtga tgaaaataca aaaagaattc
    11581 ccgatatggc gcattcaaga aaatataata acgcaatgcc ggacgcaaaa aacaggaacg
    11641 agctgaaaaa tccggcgcgt gcggcggaca ggggatttaa aaaacggcga tagatgaaaa
    11701 acgggataac gccgattgtg atatatgaaa tcgcaagcgt tatgatggcc ataagcatat
    11761 ccgccgctgg aaatatcctt attattattt catagcccgc aagaccgggg cgcatgctgc
    11821 cggaaaagat ccacggcgca agcgcgaata aaataattcc cgttaaaaac cccgcgatga
    11881 tgtcggcgta attccgtttc atgaagaatc ccgctgaaaa gatttttgga ttcttccttt
    11941 ttctcttttt ccgtcgccgc gcgggcggat cgccgcagaa cggacttcga aatagatttt
    12001 cttgacgcgc ggactccctg ctttatcgat tgcgcggatt atgaaaatat ttttgccgag
    12061 ctttgtgaaa gtaacattat cgccgctcga aaccggaacg tcgttcatgg tcgccgcgat
    12121 tgacgcgacg cccgattcgc catcaacggc gctgaacgat atcgttgccg tactgtccag
    12181 aagcggcgcg cgcggaagcg cggataccaa gatgaccggc ggcgtgaagt catttatggc
    12241 gtccggcgcg gaaagaattg cgtcgggatg gcgtattttt tcaaatttcg gtcggctctt
    12301 gcgtcccgtt tttaggtcaa tattttttcc gaacccggaa aaatcaagtt ccgcaatcga
    12361 actcgtgccg tcctgcggca ccgaaagata ggttgcctgc cggtcgatcg cgccgccgcg
    12421 ataacctttc actttcatat cgaatgttcc ggatgccgtc gcttgattga ttgctctgta
    12481 attatttccc gccggaacga gaataaaagc gttgtcgccg agcttttcgt attcgcttcc
    12541 agggatgccg agcgcgaccg ttcccgacgc cgttatgccg gtatattggt tttcggcgtt
    12601 gtacacgccg agatcggccg aaccgtgcgt gctgaattca agggttgtct cgccttccga
    12661 tgatattgtt gccgattttt gcggcgaaac gcaatcggcg gtggatgccg aaatgccctg
    12721 cggcaggggc gccgtgacgc cctcgattat attttttatg agcgtgaccg gccgcgcatc
    12781 gctcgtgagt cccgtatggt cgatgccggt ttcgccgctc atcacgaaat aattatgaaa
    12841 ttgattcgcc ctgtccatcg cgctcgtttc cggcacggtg ccatcgccgg ccgcgcgccg
    12901 aagatttatc acgccgtcat cataaagatc aaatcccgta atcgtcggct ttccgcagcc
    12961 gacgatatta tagacgtccg gcgcggcgat cggatgatcg tcaattgcgt cgtggaacgc
    13021 atccgcggca ttcaaaagtc cggcgttacg gcggtcggcg ctgtcgcttg tcatcacgtt
    13081 cgccgtcgcg tcataatcaa gaaattgttc gccgccgttc cgaaaatcct gtacgtatcc
    13141 gccattctcg gcgacgtatt tcctgctcgg cagtaattga tagagagaag gcatattctg
    13201 ggcgattttt tttatctcat cgccgtttac gaccggaata cccagatcgt cgccgtaatt
    13261 caatatcttg aacgtgtccg gcgcgccgag ctggggaact cccgcgagta tcactttatc
    13321 caggaacggc gcgttcggaa tttccgcgag gtattttttg agcaccagtc cgcccatgct
    13381 gtgcgcgatg atgtctatct ttcctgtcgg cgacgccgcg gccgcctgtg cgatcttcgc
    13441 cgcgattgcg ggaatggcgc ggttgatatc gagccgccag tcgtacggcg ccgtaaaaag
    13501 attccggttt tcaacgtagc cgtcgttttt gaacgcaccg atcaggttac cgtaaaaatc
    13561 atcgctgaaa aggacgatgc cgccgaccgt aatcgccgcc gccgataata ttcccgccgc
    13621 gcgtatcgtg ctttgggcat cgtcgccgtt ggtattgagc atcagcgcat caagataatc
    13681 atccgacggc gatacgatca tcgtgtcgac gttcggccat gcctcgcttc cgtcggacgt
    13741 tttttgaagc gccgtccccg caatgccggg aataatgacg acgggttcgc gcagcgcgac
    13801 cgtgaaatcg gtgttccccg gaacgccgaa ttcctgccag ccggatacgg cgcccgtgag
    13861 aacattttga agccgcgcgc gccagtgata gcctccgttc gcgagtccat gcattgcaac
    13921 ggcactgttt tgcgtccaaa gaaaaatatt tcctgccgcg gtcggtacgc cggtgaacgc
    13981 cgcgttcgtc ggccggacct cgacctgcag ccgcagcggt ataaaaactc tcgacggaaa
    14041 tgagatgccg agtatcgccg ttcccgccgc gagcgtgccg ccttcgagga tcgccgttgc
    14101 ctcgtcggac ctgtattggt gcaatgatcc ttgcggaaac cggaatatag tgaagaaaaa
    14161 tccgttcccg gttgccggcg atatatttgc tctattaata gatgggcgct gcggcgaaaa
    14221 gcttgcgctt aaagcgctgc tcgataacaa cgcaatagcg aacaatacaa aaatgttccg
    14281 tgtgaggtat tttaccgttg caacaggttg tatcatggga ttgctcccag taaaacacac
    14341 tgccgattaa aactatttaa agaaacgttt cgtaagtctg tttttgaggc ggactctatt
    14401 gcttcggctg cagaggggat tttttcggta ttataaagga gaatagacgg cggatgatat
    14461 aaaaaagtat ttcaaatacg tttttttgtt tctcataatc atggaatccc gttataatga
    14521 gtgcatggag gaaaacgatc aaaaagagaa gaaagagaaa ggaccgcgaa agagatgggc
    14581 cgcgcggctt tttagaatcc tcggtccggg actcatcacg ggcgcggcgg acgatgatcc
    14641 gtcgggcata gcgacgtatt cccagaccgg cgcgcagttc ggctacggcc agctttggac
    14701 ggctcttttt ttgtatcctt tgatgacggc cgtgcaggag gcgtgcgcgc ggattggcgc
    14761 ggtgacgaaa aaaggcatcg cgtcggtcgt aaaagagcat tacggccgga caattttgta
    14821 ttgcgcagtt tttctcatcg tacttgcgaa cacgatcaat atcggcgcgg atatcggcgc
    14881 gatggcggcg gccgtgcgcc tcgtcgtgcc gattcccttc gcggtcgcca cgcttttttt
    14941 tgcggcgctg atgctcacgc tcgagatatt tatttcatat aaaaaatatg cccgcatttt
    15001 gaaatggctt gccgcgagca tgctggtcta tcccgctaca ttgcttttga tcgccgcgcc
    15061 gtggggcgcg attcttcgcg ccacgttcct gccgcgcatc gagctgaatg ccgctttctt
    15121 tttcgtcatt accggcgtca taggcacgac gatttcgccg tatatgtttt tttggcaggc
    15181 gtcggaagag gtggaggaag aggtcgagca ggggattgcg aagcggcatg cggttgacaa
    15241 aggttttata aaaaatatgc gcatcgataa tgcgctggga atgtttcttt cgcaggccgt
    15301 catgtggtgc atcatcgtgg ttacgggcac cgtccttcac cggggcggcg tgacgaatat
    15361 cgcgacggct gccgatgccg caaaggcgct cgcgccgctt gtgcatactt ttcccgacgc
    15421 cggattcctt gcggagatcg tttttgcgat cggcatcgtc gggctcggcc ttctttcggt
    15481 gccggtgctc gccggttccg cggcgtacgc cgttgccgag gcgttcgatt ggcatgaagg
    15541 gctttaccgg aaattgcgca aagcgcacgg gttttacggc gtcatcacgg ttgcgacgtt
    15601 cgtcgggctt gccattaatt ttgtcggcat caatccgatg aaagcgctta tcgtcgccgc
    15661 cgttgtgaac ggcgtcgttt ccgtcccgct tatttttctc attgctttta tttcgtcgaa
    15721 caaaaaaatc atgggcgaac acgcgagcgg ccgcctttca aaaatattcc tttggatttc
    15781 ttttgtcgcg attcttattg cggcgattgc aatgttcgcg acggtattat aggacccggt
    15841 taagaaaata aataaaatag ggtaacctct cgctgcctat aaccagcgcc gattctgtcg
    15901 cggcattttc ccctccgccg tcccttcgaa ggaggggatc cgtgcttttc gtcttagttt
    15961 gtgagtttta aatcgtgaat tataaaattc ttaaagcgtg gatcctcgcc ctgtggagca
    16021 ggcaaagccg ccactccata gggcgagtct ccgcgaggac gacagaaaaa gcatatagat
    16081 cctcgaccag gccgaggacg acggaaaaga tgggtaccgg cgcgtacgtc ctctatttat
    16141 tttctctcgg cgagcgtcac ggcggcgtcg aactgttttc cgccgcggag gacgacgagc
    16201 gtgattacgt cgccgacgtt tttgttttcc aaaagatcct ggatggggcg ggtgcgatcc
    16261 agtttttttc cgtcaagcgc gaggacgata tctttttcct tcaacccggc tttttccgcg
    16321 ggactgccag gaacgaccgc gctgtcgcgc gcgctttcgc ggacgacgag cgcgccgtaa
    16381 tcgatcggca gtttcatttt ttccttcagc gtgtcgtcga tcatgatata gcggaggccg
    16441 aggagcggcc ggcggatgcg cccgtaattt ttcatatcgt cgagatcgcg tttcgcggcg
    16501 ttcacgggga tagcgaagcc gatgctttgc gcgccggaaa caatggcggc atttacgccg
    16561 atgacaagac ccttcgcgtc gacgagcggt ccgcccgaat tgccgggatt gatcgcggcg
    16621 tccgtctgga tgaggccgcg catttcctgc ggcgggtgat cggggtccgc ctgcgccata
    16681 atggagcgcg agaggccgga aacgatgccg agcgacaccg tatttttgaa aatgccgagg
    16741 gcattgccga tcgcgatcag gctttgcccg agctgcaact ttgtcgcgtc gccgagtttg
    16801 aggaagggaa gattctctga tgggatttgc aaaatggcga catcgttgat gggatcgcgg
    16861 ctcaatatca ccgcgggaaa tttctgcccg tcgttcagta tcaccgtgta ttccgcctgc
    16921 ggatcgctga taacgtgctt gttcgtgaga atgatgccgt tcgcgctcac aatgaacccc
    16981 gaaccgccgc cgacttccac catgccgcgt ttgtccacca tgaaatcggg aatatctatt
    17041 ttttcttttt cgacgggctt attgttcgcc gcgccgtctg cggcagggga ggattcctgc
    17101 gacagctctt tttcaagatc ggcaagatgt ttcgcgatgg cgatcgaaac caccgccggc
    17161 atcacctttt tgattatcgc aatcgttttt ttgtcgttca tgttcgatgt tccgttgtta
    17221 gagtgtcgta attgttctca tgaacctatc ggacattaga acattcaaac attacaacgc
    17281 gcttctttgc ttcattatag catacttccg tgaaaatcgg cggttttctc gctgaaaaat
    17341 ccttttagaa tcttttaatg ttcaatatgg cagaataaga caggttccgg gactcatcat
    17401 aaaacagatc gctcgggcgc ctgattgcgg atgttttaat atcgtcgtat tataaaaata
    17461 acacatccgc tctctttgct tataaataaa tagcatgcgc atattgctca ttgaggatga
    17521 gaagggtatt gcggatttta ttatagccgg gctgcgcgcg gaacggttcg cggtggattg
    17581 ggcgagtacc gccgaaaaag gactgcttct ggcgaaagtc aatccgtatg atgcgggggt
    17641 cttcgacata cggctttcgg acccgtcctc cggcgcttta ccttcccatg ttttgtcggg
    17701 caaaaccgtc ggcgtttcgt cccataaaat ttcggatggc aaagtctggg gcggcgagcg
    17761 tgacgggctt tgtattctgg aagaagtgcg caggaaagga aaaatatttc cgatcatcat
    17821 gctttcggtg acgggcgacg cgcagacgaa aatcgacgcc ctgaacctcg gcgccgatga
    17881 ttatctgacg aagccgttct ctatggcgga acttatcgcg cgcatccgcg cgctcttgcg
    17941 ccgggaaaag aaaataacgg agcaggttct cgccgttgcc gatcttacga tggacacgcg
    18001 tgcgcatacg gctgcgcgcg gcggcaagcc ggtaaaactg aaccgcaagg aattcgcgct
    18061 gctcgaatat tttatgcgga atcccggaac gatgctcacg cggagcatga ttctggatca
    18121 cgtgtgggac atgagcgtgg atccgttcac gaacacggtg gatgtccata tgcggttcct
    18181 gcgggagaag attgatgacg ggcgcaagaa aaaattattg aagaccgtgc acggctacgg
    18241 atataaaata gaggaatgag gactacgttt ttcctcttcg gaagcagggg ctctcttaac
    18301 tgcttccgaa gaggaaaaac tccgtttgtg gcagaagcgg aagcaggggc tctcttaact
    18361 gcttccgaag aggaaaaact ccgtttgtgg cagaagcaac ggaatggctt ttttaaaact
    18421 cctttgcgaa taagaaacgc tttgtccggc gaaaggaaat atgaaaacgg attttttaat
    18481 ataataagtc ttaatataat aacgcggtga ataacggatt gcttttgcaa tatgtcgtta
    18541 tcggccttga tctggcggtt gcttttctgg tgcttgttga ccgtccgaga aatccgttca
    18601 accgctcgtt tttcattttc gctttcggcg ccgcgctatg gatcttttgc gcggacatgg
    18661 tttcgctcgg aaataacctc gcgttcttca ggccggcatt atgcggcgcg gagatcatga
    18721 tcttgggttt cgtccttttg gcgaaatttt ttccgggcga caaagaagac aacggaaaaa
    18781 taacggggaa ttttttattt tttctccttc cgtggcttat cattttcatc cttactttct
    18841 cgcaactcat catccgtttc gccgatatca ataacgacgg ctacctcgat gcggtcggcg
    18901 gcatcctcgt tccggcgcgt tttgcggtga tggcgggata tcttctatgg agcagtttca
    18961 tatttttcgg gaagtaccgg aaccttgcgc cgtccgcgcg tgtccggaat ttttcttttg
    19021 tcgccggcgc gggcttattc gccggcgcgg tgctttcgtg cgatgttttc tttcttatgt
    19081 tccgcatttt cggcatggcg atgatcggca gtctgttttc cctttttttc acgggatgca
    19141 tggcgtacgc gatcatgcgc taccggctca tggatatccg gctgatcgta aaaagagggg
    19201 cggtctatgt tatcgccata ttcatggtcg ccgtttttta tttcggagca tcttttttcc
    19261 ttcaggaatt tcttcatgat aacgacgccc ttacgtatcc ggcaagcgcc gttgcggccg
    19321 ctgtcttgtg cgtgttcggg tttctctacc ttaaacgcga attcgaggaa ataaccgacc
    19381 gttttttctt ccgcggtgaa tatgattatt ttacggctgc gcgcgacctg aggaatgcat
    19441 tttgctccac gattgccctc gatgattttc tcgggtccgt aagcggtgtt ttttccgata
    19501 ccatcaagcc ggaacggatc gttttctttc ttgataccgc gcatcagccg tattttttcg
    19561 acggcgtttc gcggaagccg ccgccttccg ccgccgaaaa ccgttacgga gaactgatta
    19621 aaaacttccg tgcgcttcct tcggaacctg tttttatgga agagctggga cgcgccacga
    19681 acgatgtcca gccgcggcgg aggaaaaaat acgaatcgct ttgcgtcgcc gcaaaaagag
    19741 cgcgcatcgg cgcaatcatc ccgatttttt cgaaagaaaa aataagcgcg attttattgc
    19801 tgggcgataa gcggtcggaa gttccgtttt cttcgaaaga cagggaatta ttgtcgatcg
    19861 cttcgcatca ggcgggcatg gcgatcgaga atgcgatgct gtatgcggcg ctgcgccgcc
    19921 atacggaaga acttgaacgc cgcgtttacg aacgcacgga gcggataaaa aacatgtacg
    19981 acgggcaggc gcgatttttg gcggatcttt cgcacgaatt ccagacgccg atttcgatat
    20041 tgaaagggaa tatcgaacgt ctggagaaaa taaacagcac gaaaggcagg gacgaatttt
    20101 atgcaatgga aacgacgctt gaccggcttt cgcgcctcat ccgcgacgtc ctgggtctgg
    20161 caaggttaaa ttcttctaaa acaaaccttg cgaagaagcg ggtcaatgtg gaaaaattga
    20221 tcgaggaggc gtacgatgac tgtcttactc tcgcgaaaaa caaaggcatc gcgttgtcgt
    20281 tctcaagcga gaaatgtttt atttgcggaa gtgtcgataa attaaaggaa gttcttttga
    20341 acctgatcgg taacgcattg aagcatacgc ccgccggcgg cgccattatg ctttccgcga
    20401 aagccgtcga tgacgaggtt gccctcgcgg ttgcggatac cggatcgggg atttcccata
    20461 aaaacctccc gcatgttttt gaaaggtttt ataagattga cggcgcgggt tttacgggaa
    20521 cggggcttgg tctttttatc tgccgccaga tcatcgaggc gcataacggc acgatcattg
    20581 cggagagcca gccggggaac ggcagccgtt ttatcattca cctgcctgcc gcgccgttac
    20641 gcaatcggaa aaaatttgat ataatataat cattcatggc gcaagcaata atcgagacca
    20701 aagaaaagag cggcatctac cttcaaaaag aaggggacat gattttggac ggcggggata
    20761 agacgtacgt tttaaaactg cgcgatctgc cggcggaaga aaagccgcgc gagaaactga
    20821 tcaagcacgg tccggcgaac ctctccgtcg ccgaactgct tgccatcatc ctgaacgtcg
    20881 gtacgaaaaa agaggatgtg cttgcgatgt cgcgccgcct gctcaaggaa tacggcgaca
    20941 gtatcatcgt caatcagaaa gatccgcaaa aagtgagcga acttttggga attcccattg
    21001 cgaaagcatg ccaggtggtc gcatgtttcg agctggggcg gagatttttc aagatgccgg
    21061 acggcggcgc gccgatcgtg ctccggacgg cgcggcaggt gtacgaccat ctgaaagata
    21121 tgcgcgacct gccgaaggag catctgcgcg ggctgtatct ggacagccat taccagctta
    21181 tccatgacga agtgatctcc atcgggagct tgacgtcgaa catcatccat ccgcgggaag
    21241 tgttccggcc ggcactggaa tattcggcga cggcggttat cctcgcgcat aatcatccgt
    21301 cgggtatcgc cgcaccgagc gagccggata tcgcgatcac gaagcagatc atcgaagcgg
    21361 gcaagatcct cggcatcagt ctgcttgatc acgtgattgt cgcgaaaaat aaattcgaaa
    21421 gcattcccgc ggagtacgag tgataccgat catcgtttaa gcaagttttg tttcgaccgc
    21481 cgtcccattt tggcggccgt caagatggga cagcgggtcc gaaaaaggtg atttttatga
    21541 ggtcatctat ttttacggcg catgatgata tataatggct tttatggttg tttactcttc
    21601 ggtcagaaag gcatataaga agcgcgtggc ggtcacggat ttttcgcttt cggtttcggc
    21661 gccgatattc ggatttttgg gcgcgaatgg cgcggggaaa acgacgatta tgaaaatgac
    21721 cgtcggactt ttggcgccaa cgagcggcac gattatgatc gacggacagc cgtcatccgc
    21781 gccggtttcg aagaacaaga tcgggttcat gcccgaaacg ccgtattttt acgaaacgtt
    21841 cacggggatg gaatttatga aattctgcgg agaactttac ggtcgccgcg gctctgcctt
    21901 caattcgttc gcgggcggac ttttgaagaa agtcggcatt tacggcgcgc gcgattatca
    21961 gatccggacg tattcaaagg gcatgcgtca gcgtctcggg ttcgcgcagg cgcttgtgaa
    22021 cgatcccgaa tatctttttc tcgatgaacc gctggacggc cttgatcccg tcgggcggcg
    22081 cgagatgaag gacgtgatgc gcgatctccg gcgcgacggc aagaagatat tcctcaacac
    22141 gcacattctc tatgacgtcg aagagatgtg cgaagagctc ggcgttttgc atcgcgggcg
    22201 tcttctctat gccggttccg tcaaggaatt ttgcgcggga aaatcgctcg aagaacggtt
    22261 cgtcgaagtc gtcggagcgt cgggttctca agagttgtag tgccggagtg ttctatggtt
    22321 gtcattgaaa atacaatact gcgcacaata aaacattaga acattagaat attttatcat
    22381 catgcatact gtccttgtca tcgcaaaaaa tacgttcaag cttgagatcc gcaacaaaat
    22441 actctatgcc atcctcgggt tcggcgttct ttatgcggtc gtgatgcttt ttctgtcgga
    22501 tctcgtgctt caggagctcc ccatggtgaa aagtttcggt cttttgggaa tttatttttt
    22561 caatgttatc gtggctcttt ttctcggcac gacctcgttc cataaagaca ttgagcggaa
    22621 gatcgtttat ttcatccttg cgaaaccggt ctcgcgggcg caattcgtca tcggaaaatt
    22681 cacggggctg tgccttgtcc tactctcatc gagcgtcatg ctcggcgccg tatattgcgg
    22741 cgtcatcgca tacgcgcacg gagggttcga cgccatgggg cttcttgcga tcgcgatgca
    22801 attccttgaa atggcgctct ttctcgcgtt cgcgattttc gttacgacgt tttcaagttc
    22861 gctcttgtcc atcgtctaca cttccgccgt gttttttgcg gggcatatcg tgagctcgat
    22921 ccttgtcgat gcgaaggcga tcgggatcgg cggtttcaag cttttggccg tcgaggcaat
    22981 gtattacgtc cttccgaacc ttgagaaatt caatatccgc gatttcgccg tgcatcccgt
    23041 cgcaacgccg ttcacttttt tcttcatggc gttgtgttat gccgccgcgt atatcgcgct
    23101 tcttcttttt gccgccatct ggattttcga gaagaaggaa ttataaaaga tgttttagtg
    23161 ttttatatgt tatggcgcaa cgaagaacgc agcgaaaacg ttgtatcatt acatcatgag
    23221 aacattgcaa caccttttcg cattatgaaa accgacattg catttaaatc gatagtcgtt
    23281 gccgcgcttt tcgccgccgt tatttttttc cagtacggct atgacgcgac ggcggttgca
    23341 acggcagcag ggaccgcgcc cgtgcagctt tctcccggct ttgtccgcac cgtcgatatg
    23401 gggttcaatg cgcccatcgc gtcatttgcg tggatcggca cgatgccgga tatcatcgat
    23461 cttttcctga acggcaagac ggaatatttt tccggccttg atttcgtgac gggcgtcgat
    23521 ccgaagctga gctacccgta cgctttttcc gtcatcaccg ttcccgccgt ggcgtcgtat
    23581 ccgaatgccg tgcagacggc gaatgcgata ggcgcgcgcg ggctcgcggc ggccgatccc
    23641 gactggcgga taccgtttta catggcgacg aattattttc tctatctcaa ggacaaggaa
    23701 gacgcgctcc ggtattacga cattgccgcg cggacgcccg gcattccgcc ggtctcgcag
    23761 cgctttgcaa ttaatttcag catcggattt gggacgcgcg cgcagatgga gaatctttgg
    23821 aaaacgatcc gcgacacgac aaacgacccg tccatgaaac agcgcgcgca ggcgtatatc
    23881 gaccggttcg atgcgcttga ttatcttgaa gccgcggcga agcaatacaa aacggagtac
    23941 ggcgcgtttc cgaaaacgcc gaacgatctt gtggcgaaac atatcatccc gcaaattccg
    24001 caggacccgt tcggctatgc gtttattttc aacgccgacg gtacggcgac cgtcgacatc
    24061 gcgcacccgc cggccgtgat cgggcagtga cgggcttttc aggagatttt cgtccgcgta
    24121 agtaaaactt aatatttgat ggtatattta
//
LOCUS       DAIDDT010000004        23998 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_4_length_23998_cov_6.257849, whole genome shotgun sequence.
ACCESSION   DAIDDT010000004 DAIDDT010000000
VERSION     DAIDDT010000004.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23998)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23998)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23998
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4_length_23998_cov_6.257849"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5971 a         6488 c         5721 g         5818 t
ORIGIN      
        1 gccggactac atcccggaaa ataaccggaa aatgccccgt acccggcgca cgtgcgtacg
       61 tcctaaaaat aaatttatgc cgacccatta cgaaaatcta aaaaaaataa acgaaaagga
      121 cggcgtaatc gctttccagg cggatatccc actcgcaatc cttgaaaaaa atataggcga
      181 ggccgtaatc cgcgaagccg ccgattttgc gctcccggga ttccgcaaag gcaaagtacc
      241 ggaacatatc gtccgccagc acatcagcga aatggacctt cttgaagatg cggcgcacgc
      301 agaattccgt gacgccatcc gcgaaataat cgccgacgaa aaactttcga tcatcggtgc
      361 gccgcaggtc acgattacga agatcgcgct caaaaatccc gtcactttca cggcgcggtt
      421 cgccctttat ccggaattca cgctccccga ttacaaaaaa atcgggcgcg gcatcgcgga
      481 acgaaaagaa ccgtccggcg ttgatgaaaa agatatcgat accgcgatcg cgcgcatcca
      541 aaaaatgacg gcaccacggg aagatcgcga aacagacacg gaaaaagaaa aaaacgccaa
      601 gggaagcggc acggaagatg aagataaaaa ggaaaacgct cccctcccgc cgctcacgga
      661 cgatttcgtg aaacagttcg gaaattttga aaacgtcgcg gcattccgcg cggaggtgaa
      721 aaaacagctc gaacaggaaa aagaaacgca gatcaaagaa tccaaacgcg aggaaaccgt
      781 caaaaaaata atggcatcat cgaagattaa aattccggcg ctgcttatcg acgaagaatt
      841 ttacgatttc atggagcggc gcgacgcgga acttgaacgg gcgggcatgt cgcttgagga
      901 atatctgaaa gaaatcaaga aaacggagaa tgagctcgaa caggaagagc ggcaattgat
      961 cgaggaccgg ataaaaatga gtttagttat gggcgaaatc agaaaaaaag aatctattga
     1021 agcgaccgag gacgaagtcc ataaatacat tccctcgctc aaataccgct atcccgaccg
     1081 cagcgaagag gacatgcacc ggactgcgga ggcatacatc atccaggaaa aactgttcga
     1141 tatgctggaa ggaaaaaacg aaaccggcga tgctgttccg ctttcataag aaaaaattta
     1201 tagccgtatg gattgagagc agaaagtcat aggttttctg gtagatgata aggtcgtcga
     1261 acattgccgg aaagaaattt cgaaaagtta aaaaacacaa agaaagaagg ttcactctat
     1321 tgaataacct gattttcgac tgtatccggc ggggagaaag aaagaaagtt aagagggaaa
     1381 gaagaagaga agagaaaaga aaagaaggaa gaaaaaccaa agggacaaag agacgaattt
     1441 tatttagtcc ttgatgcaac gggcagaaaa cccgtaggcg cggtaatcgt aattcgcggg
     1501 gaccacgaag ctcgagtgga aggcgaagtt gtaggcgttc gtgccgtcga tggaagacga
     1561 ccagtagtag ccgctcgcgc cctgaccagc aaacaaagcg tcggagggat cgcggagacc
     1621 ggcatcggtg agtttgaggg ttgtgttgta caaagccgta tcgcatcccg aggagcagga
     1681 agcaaggccg acggcgctca cccaattgct ccattgcgtc tgggctggga tgctccatcc
     1741 gatcgggcag ggattgttga tggcggtgct ggtaccctgc cagaggttgt cattctgggg
     1801 ggaacgccag tcataagggg aattgggggc ttcgatgaag tcggcattgc cgggaacatc
     1861 ggtgctactg agggtggttg ttgcggtgct ggtcgttatc tgatggccgt ccgtcaggcg
     1921 tccccattgg tagagccatc cataggcgga ggaatcggcg gcggattgtg caatgttagc
     1981 agtgccgaga ttactggcaa gccagcaatt gccgtccgcg ccaaggacgg ttttatatat
     2041 ggtggtatca ggagcggttc ccgtaacagt gctaacgcca caagtgaaga tcagtgatgc
     2101 gctggtccct acagtaaatt ggggagattg cgcttggaat ttctgcgatt ccgcattagc
     2161 ggagacttcc caataggtgc cgtcggtttg ataggtgtaa tagaagcccg atgaggcggt
     2221 attgatagga tctacaggaa ggacggggag cggagaacct atggatatct ttgtcatatc
     2281 aacaggaatc catccggtac tgtcggattt tcggtaggtg gatgatgcgg cgcaatgggt
     2341 cgttcccgtg atgcccgaac agcttcctcc cgttgtccct acggtagggt caagcaggga
     2401 gagataggag gtcgatgtgg cgccaggagg cgtaggggaa acatcattga aataaagctg
     2461 tgtcgccgtt ctgatgtttt ccaggtcgga aagacggttg ctgtccctgg cctgcttcat
     2521 aagttcagaa ggattcatgg taatgatgac cgctactgca aggacggcga tgatggcgat
     2581 cacgacaaga agctctatga gggtgaaggc ggaacgggaa tgtattcttt ttgttgcatt
     2641 catggtcttc ataagagaaa gagaagatgg agcattattt agatgagact gattcgtctc
     2701 tcgctcgtga taaatatttt ccttttttga tacggtcttc acaggaaaga tgttcatgga
     2761 aatgacgata ggtttgattt attgtttatt acgactgaac gactttttat attttatttc
     2821 attaatttta atatgtttta ttttttatta ggatagctta cgttattatc tcttcattat
     2881 tatagcatag atgggaaaag aggggctgtg cataagtcgg gggaaaaaga agttaactga
     2941 taattatatt tttttcagcg tcaggtttat gacaagacgg ccgggcggat cggccgattc
     3001 gtattcgtag gtcggaagat tattttcggc gaaccggaaa ccgatcggcg ccataatgtc
     3061 gaaccggtag tgccgcgcgg tgcccgcctg cttttcaaaa atgaattgat agctcatgcc
     3121 gtccgcgggc ggcaaaaaca gccggtgcga ataatcgaag gaaaccgtcg tcgtattccc
     3181 cgccatgacg cgcgcccacg tcgcaaaaac ttcctttccc gattcttcgt gcgacgaaac
     3241 cggatacccg aaaatatttt tctgcgtcga cgacagcgcc gacaccatcg gatcggtgct
     3301 gtatcccatt ttcgcatacg caaccggcgg cgcgatattt ttcgcgaagc cgccgctctc
     3361 attggataag gtcgacccgt cggggacgaa cagctggaga taatcctggt tcggcgcgcg
     3421 gtaccaccag tacggactct ggttgccgtt atgcttccgg ctgatgataa cgttgtcgct
     3481 taccgtgccg tccgcgttaa tctgactttc aagatcgaca ttttgcgcga tataaagatc
     3541 cgacttttgg cccacgatgt tcgcgtccac gacggcgaga taatcccctt caaaatcctg
     3601 cggcaagacg gcgaccgttc cggcggcgtt atattgctcg aaaaaatttt caaacgccgg
     3661 atttttggcg tagaacatga catctttgcc gtttatccag tccgtcgcca tgggaataag
     3721 cgcctgcttt tgggtatcgc tcagcgccgc tattttttgc gtcatcgcct ccataagttc
     3781 cttaagcacg ctcttcggat aggtcgcgcc ggtcgcctgt ccgttttgca ctattttctg
     3841 aatctgaaca aggaacgtag cggcatcgaa aaccgttcgg gtactcgaaa cggaaatcgg
     3901 gccggtcagc gacaagaggt cgcttatcac tttcggagaa acggcgatca ccgcgtcgaa
     3961 tgtttctccc ccgcccgccg gaattttgct ttgtaaacca cctgaaattt tgttctgtaa
     4021 aatttcggat ggtgaaattc gagagggcac gccgttcgcg gtaccggaat ccgaagatga
     4081 cgccgcgtat aatcccgacc tctcgaaaaa cgaaacggtc tccgacgccg aatccgcgaa
     4141 atcgaaaaac cagtttgcgt cggcgggacg aaaacgggaa acttcggcct gtaaaggcaa
     4201 tggaggaacg atattttctt tgaatgtcat atcgacatcc gccacgtcat gcacggcgat
     4261 gtccgtgata ttcccgccct gaagcgtgac gtccgcataa cttcccagaa aaccgccggc
     4321 aggacggatc tccgaaggat tctggaaaag cacgagcacg tgatgcggcg cggacgcctg
     4381 gagccacggc acgaacgcat ccaaaaaatt ttcggtattt tccattttcg tccttaaaga
     4441 caagagatcc gttcccgccg gcatggcgct tcccgcaagc gtgagtgcgc cggaaatacc
     4501 gccgctttcc gcgtcgatcg cgtgcaatgt accgcgaatg tccgccagtt ccgcgacaag
     4561 attgccgccg ttgcgggtcg aacaaaaatc gaaaaacgat gattcaagcc cgttcaattc
     4621 atccgaaagc gacgaaagat tattcgagat atcgacaaag gaatggaacg gtccgccatg
     4681 ctcgaacaag aaaccgaatg cgccggcggc gtttttgagg tcaagcgaca atccttcatt
     4741 cgatgccgaa aattcacgcg ccgcatcttt caaatcgaaa ttctgcaaat cctgcgcgcc
     4801 cgcctcaaga ctgccgatac gggtcgaaac aatgcggacg gcgttcaggc gggcggaata
     4861 aaaaagaacg gcgcatcctc cgataaaaaa aataacaaga ccggccattc cccagacgac
     4921 gccacgggga agcgaacggc ggccttcgtt ttgaaattcc tccgccggcg ttgttttttt
     4981 agcggcgcgc cctgttcgcc cgccaaaatg ccgcgcaccc gtcgagcggg taaaaaaatc
     5041 cctcacgccg ccatggcggc tccgcggttt tatgtcgtcc attgcgactt ctttcgcgcg
     5101 cattgcttcc ggcgatttcg gcggcctcgg aatttccatc cgttccgctt ctttttcaga
     5161 tcgatgatcc atgatccgta ttttaccata cccgcggata tccgatgttt atatttttgt
     5221 catcgtccct ggccaagccg gacgatgccc gcgggcggat atcaccttat gctataattc
     5281 cactatgctc aaaaaaatat caacgcgact tgccttgatg acactcggca tcgtctgcat
     5341 catcgctttt agttttatgc tgtgggcggc gaacaccgat tccgcgatta tggacgagct
     5401 tgcgcatatt ccggccggct acggctacgt acacaatctc gattaccgtc tcaatcccga
     5461 gcatccgccg ctcatgaaag cgcttgcgat gctgccggtg cttttcctga atccgacgtt
     5521 cccgaccgga agcgacgcat ggcaaaacca ggtaaatgcg caatgggcgg tcggtacgga
     5581 gttcctctat caatcgggca atgacgccaa cgccatcatc cggaccgcgc gcatattccc
     5641 gatactcgtc accattctcc ttatcatcct catctatttt ttcgcgcgcc gccttatggg
     5701 cgactggtgg gcgctcctgc ccgcgacgct tttcgcgctc tcgccgaacg tgctcgccca
     5761 cgggcattat gtgacgaccg acgtcggcgc cgctttcggc atcgcgctcg cgacattttt
     5821 tttcctgaag ttcatcgaca cgccttcaac caggcatctt tggtacgcgg gactcgcttt
     5881 cggcatcgca cagcttatga aattttccgc gccgctcctt gtcccccttt tcatatttct
     5941 cgccgccgtc ctctggatcc gcgatgttgc cggtcactgg aacggaagtg aacatcgcgc
     6001 gcggcttaaa aaattcatcg ccgccggctg gtattattgc cgcaacgtta ttttcatttt
     6061 cacaatcggc tatgttttta tcgtctatcc tgtttatttt attttcacgg cgcattatcc
     6121 ggcggcaaaa caggcaagcg atacggtatc catcctgaat tctttcggcg gcggcccgac
     6181 gcccgcggga cagtcctgcc acggctcccg ctgtcttgcc gacctcgata tttggatgac
     6241 tcataatccc gtcctgcggc catacgcgca atatatgctc ggcgtcctta tggtgctgca
     6301 gcgcgtctcc ggcggcaaca ccatttattt cctcgggcat gtcgtgggaa gcggcggatg
     6361 gatctatttc cccgtgctct atcttctcaa agaacccatt cccacgctca tcatcgtttt
     6421 cctcgcgctc gcgctcgcgc tgtggggtat cgccaaaaaa acaatcgccg cccgcggtcg
     6481 cgtgaaaaaa atattcctcg actacctcgg cgtgaacttc gcggaattcg caatgctgtc
     6541 attcatcgcg ctctattggg gctacagcat gcacagcacc ctgaatatcg gcatccggca
     6601 cctcatgccc accatcccct tcatctacat cctcgctgcg ggcgtctgga aaagatggat
     6661 tgcgaagata tatttgccga ccggagtgtt cacgccggca tcgcttgccg ccgccgcgcg
     6721 atcgttcttc gtgtccgcga taaaatatat ttttctcgtt atcctcattg cgtggctctt
     6781 tattgaaacg gcattcgcgg cgccatattt cctttcctac ttcaatgaat tcggcggcgg
     6841 cgtcgcgggc ggttatcatt acgtcaccga ctcgaactac gactggggac aggacctgtt
     6901 gcgcctccaa agctgggtcg ccgcgcatcc aaaggtggat aaaatcgccg tcgattattt
     6961 cggcggcggg aatccgtcgt acgctttggg cgcgaaagaa gtcgactggt cgtcgtcaaa
     7021 aggcaatccg gccgatcacg gcatccattg gctcgcggtt tcggtaaaca cgctcgagct
     7081 tgccacgcaa ccgctcggtc aaggacaaac acgcaatgct tccgacacgt atgcgtggct
     7141 taccgcactc cgtcccgtga aatccggcat gggcaacgtc cccgaacctg atttcaaagc
     7201 cggaacgtcg atcttcatct atcatttgta acctgctttt cttcgtcgtc cgatattctc
     7261 ttttccgtca tcctcgacct ggtcgataat ccatgcgagg acggcaaagc gaactgatat
     7321 ttttattctt cttacagcac gccccaggtg tgaagctcgt cgctttcttc ctgccatttc
     7381 ccgccgatgt cggtgcggta aaacatgttc tgcagcatga tattgtcgat ccgccgatac
     7441 gcctgccggc gcgcgtcttc cacagtcgaa ttggagccgg tcacgataag cgaccagccg
     7501 gtcttgccgg caagcttcca gtcgttatcc accagcttca cgtcgcccag atggattcct
     7561 tcaatgtttt gttttttaaa aaggatcgac gaatccttat aaacctgaag cattttttcg
     7621 tcatgatacg ggaacggcgg cgtcgcgatc acaacgccga tctggaaccc tttctgcgtt
     7681 ttcagcggat acggctcgcc tttcgcgagc gcgtataaaa attctcccat gggactcagg
     7741 atgccttcca tctggatact gatcgtggga tagccgaaac gggacgtgaa ctcaagagga
     7801 taaataccct tcccgttcac gatgcaattg atgtcgatat acccgacata cttgctcgca
     7861 cggagccgct cgagcattct tccgagcgtc gcgttgaaaa aataattcgg ttccgtccag
     7921 aacataagcg tccccatctc gcccgtataa gggccgatgt ctcccggaaa aagtttttta
     7981 tgctcgaagt tgacattgac cggcgtgatg aaatcctggc cgttgaaaaa cgctcccacg
     8041 gcgatctcca cgcccgaagc gaacttctgg agctggaacc gcttgatttt gcgcgaccag
     8101 ctttttctgt tgtgctcgaa cacttcaaga agatccttgc cgtcctcttc ctgcccgata
     8161 aaaaggaggt tcttcatgtg cgaatcaaaa ctcccttccg acggcttgaa gatataccgc
     8221 ccgggcgtcg tttttaagaa ttctatcgcc gcgccgaagt tatcgaaatc ccagtgcggc
     8281 agcgtaagca tgccgaccga tttcatttcc tgctgtccga actcgcggtc gtattcgagg
     8341 cggtcggtat aggcgcttcc gccgatgacc agcttcccct gcacgcgcag gtcgtcggcc
     8401 gtttttccga ggttgtgttc cccgtgcagg acatcatcga agacaacgac atccgcccag
     8461 tcgacggacg tccgccagtc atcggacttt tcgacaaaac cgtcaccgac atcctggtat
     8521 tcctcttcct cgatatacat cctgacttca ttcccttcct gcttcacctt ccacgccaga
     8581 tcggtgccgt cgccgtcgat ggaaataaaa aggaatttct ttgagaattt ttccggtacg
     8641 gcgggatcct gcgtctttgg cgacgctcca tcaaatatcg gaacagattt tatctcttct
     8701 tcgctttgca tgattttttt atgttacagg atgtgcgcgc ttgcgccgag tacgaggcat
     8761 tttcttcttt gtcatcgtcg ggcttggccg gacgacctat gctttgaaat taagaaaaaa
     8821 aatgaaagca tggatcctcg ccttcgcgag gacgacgcaa taagcattgc gccggatggg
     8881 aagcgcgggt cgtccgtcgg aatttaccct ccgcaacgat cgaggccgga cgatgacaga
     8941 gaagaaattt tcttatgatt ttctttcaaa cgcgtacgtc ttattttaat actcccgtaa
     9001 gaataaacgc aatagtcaat gacggcattt caacgcctca ccaccgaaaa aacatgggtc
     9061 ttataaaacg attcgggcat gaattccttt ttaaactcca atccccctcc ttcttcaacg
     9121 ccgcccatgt ccacctcgcc gtagccatgg ctcttcagcc atgcaagatc ttcgagataa
     9181 agcatcgctc caaggttggg catcgaataa tcatggatgc ctatggcgcc gtaaaaccgg
     9241 tcgctattgg ggatcatcca tccggcgttg atcccaacgg cctttccgtc gacgataaaa
     9301 acccgggcgg tcgatgtccc ttcgaaattg ccgtcgatca cgtgataata ctccgcacaa
     9361 tacgccctgt cgtgaacctt ccttttcttt ttccagacat caatgattgc gcgcaagctt
     9421 tttttatctt cgaatttctt cgcgtccgca accgtgaccg tatgatccct gtaaaacccg
     9481 ttcctttcct tccgtaaaaa tttatagtgc ttgccgggca gggtaatatc gaacgtcttc
     9541 atgttcataa ccggccaaac aagcgtgtaa tttatggggt tggcatgcgc gcgctccggc
     9601 aatgcccgca acagctcttt ccgcagcgac gtttcgagtt caagccaaac tttctttata
     9661 tccggcattt cgcggaatat ttgcccgata tactcagcaa gaagaaacac ccggcgcccg
     9721 ggagacgcaa caggaccgga gaaaacgcgc gcttctccat cactcgtcat ggtaagaagt
     9781 ccttcgccgt gatcacccgc cgcaaacaca tccctctcgc cgtcatcggt ataaaaccgg
     9841 taccaatcaa aattatgctc gggggcatag ccgaactttc gaattgcttc ttcaatgcgc
     9901 ggccgctctg caatgacatc atcaaaaaaa atcatcgtga ttgcctatat tagaaataat
     9961 ttcccttgct gtcatcaaga gttttataat caatcaataa ttcatcccct ttcttaatcg
    10021 gccgtacggt aacgaaatca tcgccgtcgt ttatcgaaac gatgttggga ttttttgaat
    10081 gatttaaaaa taccgccata tcccatattt tgaatccgta acgcggcaac gtatatttat
    10141 tttttacgga aagacagtag gcatcaatca gcgcgcgcac ttcccgcggg atttttttaa
    10201 actcgctttt atccacatca atccagtcct cctgccctgc cgcaaaagga tgcgttcctt
    10261 tgggaatgtt ccgtatggcg aaaacaccga cgccttcaat atcggacggc gcaagcttgc
    10321 accacgtatc ggcaagatct tttaataacg cgcgtttgtg cgccacataa tcctttttca
    10381 tgatagatag tatgatacaa tagtatcata ctcttaagat tttctgccat gaaaaaagac
    10441 gctatcatca acgatctcga aaacaatatt tattgccgga tacaacgatc gccgatccac
    10501 ggcgtgggcg ttttcgcgat aaaaaacatt ccgaaaggcg tgaatccatt cgtcacctat
    10561 ggagacgtcg acgtgatccc cgtgcccgag aaagagataa tggaaaataa aaaaataccg
    10621 gacgccgtga aagaaatggt aaaagcattt tacgtcatcc aaaatggcgc tatttactgc
    10681 gatgcgcgga gcctgaacga gatcaatatc acctatttcc taaaccattc gaacgctccg
    10741 aacctcgacg tcgaagacgt caacgaagaa agccttttca ccgccaaaag agacattgcc
    10801 gcaggcgaag aactcacgtc cgattattcc caatacagcg attcatattc tttaccgtaa
    10861 ataaaaatac tgcaaccact ttatatgttt ttaatcccct cttcgtggta caccgtcaaa
    10921 aaaacggaga agaaagggcg cggcgtattc gccacgcgcg ctattgaagc cggcacggtc
    10981 atcggcgact atctgggaac cctcatcaag ccggacagtg ccgatgaaaa aaaacacggc
    11041 ttgtacgata tggcaggagg aaagaaatat gatattttag ccgaccccgc aatagaggga
    11101 attcatttcg tcaatcattc atgcgcgaac aactgcgata tctacccgta tcagggacac
    11161 atgctgctct ttgcattacg taaaatattt aaaggagaag aactctccct tggctactgg
    11221 atgtatgcgc cctatgatac cgaaacaacc tgcgacatgc atgcgtgtta ttgcggaagc
    11281 gagctgtgca cgggaaccat gcaccacgcc gccatgaatt tcgacctgtg ggaaaaatta
    11341 gtcaaaaaag aattcggaca gtggtatcat aagatccccg gaaagtacgg cgatcggctt
    11401 ttgccgcttc ccgcatatcc tgcctccatc agggatgaca agaaaaaaat atatgactat
    11461 aatcttttcg gcgccgcaac caaatttcct gaaaaataca aagacgcaac actccccacg
    11521 atgaccgaac tccggaaaag aatcagaaaa acaggacggc gtctttcgtt cccctctctc
    11581 aagataaccg tttatggaat ccgggacggg atactcctgg cgaaaagagc gcacgagcat
    11641 attcgattat cccaaaccag tccgcgccgc cgcgcgacgc tatcgaaagt tcaatgctga
    11701 acttcatttt tcgattctga tccgcgcctc atcatcgaat tcgtaataat tttccgtcaa
    11761 ttcctcccct gcctgaatgt ccctcagcgc atacatttcg cccatcgcat cgtcgcgcga
    11821 cggatcctcc ccgacattcg gatcatcggc gtgattcacg aaacgctcgt catcaggatt
    11881 taatgtatac aaattcgcct ccctatcgaa gtacgcgtaa ttcaaaaatt gttcccgcgc
    11941 cgcgtcgggc aaagcatcga ctttcccctt cggaatttca aggtcgaatc cctcgataaa
    12001 ttttcgcacc agcgcaccct tcggaatgaa ctgatccgca aaacatccga tgccgtggat
    12061 ggagctggaa ccgattttcg tttttataag aagcatgatt taaaaaaaat taaaataaaa
    12121 aaatcctaac gctcaacgct aacttcccaa ttcttgaatc tattttctaa taaggcaatg
    12181 atttcacgaa agatatgaat tcctgcgacg cctcggacgg cgcgcatcgt tttttaaccc
    12241 atgctgcggc tttgcgctcg gtcatagttt cgtcatcata aagcgcaagg acttccgcgg
    12301 caagccgctt ttccgcggca taatacgcat ccacattttg tatcgccagc tttccattct
    12361 tgaactccac gatcccctgc tcgaaaagac tattaagatt ataaacctcc gccttgatgt
    12421 atacgtcagg ctcgtttttg aggcacatca caagccccat taatgtcgcg gcagcccatt
    12481 gctctttgtc gccgaaaaaa ccgtcttcca gcatatcggg aatcagtgcg cgccatacta
    12541 tttccgcctt cacctcgtcg atgatcgtga gcggcctcct cccgagacgt cggccctgct
    12601 cggtttcgga tggatgcact ggatggctga actcgtgaag catcgtctcc gtgcgcgaca
    12661 tcttttcgag atagtcgatg tttgcttttt ccggcatacc ggaaaacact ttatccgcgc
    12721 cttcgataac attccgatat gccggaaata agcgatcgta tcgcctaatc tcttcatcca
    12781 gaaaaagcga atagaccgga tcctcttctg cggcagcatt aaacgtaagg ttagcgccaa
    12841 aaccgccaag aatgattgaa ctttttacgc ccattttccg gacattcccc gcgaatgccg
    12901 ccgcgccgat aacgccgagg ttatccgcca tgatatccct cgcgcgcgca aagtccttcg
    12961 tttcgcgatt cgcttccgcc gtggcaatgg aaaccttaaa ctcaggatcg agatacggtt
    13021 ctttataata tcccgttccg gacgctccta aaattatcgg aaaatcagtc gcaacgactt
    13081 cggcatagcc ccgattgatc tcatcctgaa tccgcgcggc ttcttcggta tttttcgatt
    13141 cgtaaaatcg tttcagaact tcgatataac gcgccatggc atcgccgccg ggacgttttt
    13201 tccatgcggc gtccttttca attattgccg taaaacgttg tgcgataaat ttcgcttcgt
    13261 gcggaaaaag ctcggcgatc gtatgcgttt tcaaatcccc cggaagatgc aaccccgtcg
    13321 gcgtcgccgc ttcgggcggg tttccgctca actgggaata tgccaggaac cgcacaaaaa
    13381 gaggatcggc cttcgctgag gcatcgagca ggtcgacgat aagatcgcga agggacggat
    13441 cgatgccgag ttcgcctagt tttttgggat ccgcttcacc caaaagtttt ttctccgcca
    13501 acgtttcgat ccgcttcacg acatgcgccg cgaccgtaag caatacctcg cgccgctgtc
    13561 cgcctatttc ttcaagttcc gaaaaccaat tatgcgccag cgcgtgaagc aatttttcct
    13621 cattcaaatc ctcgatggac ggaaccgctt ccttcagctt cggaattttc ttaatgagat
    13681 aatcgcatac cgcctcccgc acgttttccg tgaattcgta catcgtatga tacggcttca
    13741 ccttttcata cagatctttc cagttctcga tttcgccgtc gggacgttct tccggctcca
    13801 aaatacggag cgttttaaga atgccgcgca aatgcggttc gggaccaatt agtttttccg
    13861 gcggtttttc cggttcgttc gaccggattt cttcgggagc attttcagaa ttgcgaaatt
    13921 tattcatccg tagtcctata gcccgtccca aaagcatctt ttcaggcagc gtccgctggc
    13981 ctgcttttga gattggtttc aattatatta ataattcacg gcggcgttta cacgtcaaga
    14041 ttctgcaccg ttgcggcatg catctgaata aaattcttgc gcgcgggaac gtcgtcgccc
    14101 atcaggatat cgaaaaactt gtcggcggcc tcggcatcgt caaccgtcac ctgtttcata
    14161 agccggtgtt cgggatccat ggtggtttcc cataattgaa tggcattcat ttcgcccaaa
    14221 cctttataac gctggatgct cggcgtcctt ttcgaaccgg cggcttcccc ggttccctct
    14281 tctgttgtct cgacggcttc ttcgccgccg tcttccgttg ctgccgcttc ttttttcttc
    14341 gccgccgctt ttgatgccgc tttcgcgggt gccattttcg ccatctcctc cattatttta
    14401 tctttctccg catccgagaa cgcgtaacgg accgttttcc cgagctggat gcggtacaac
    14461 ggcggctggg cgatataaat atagccgccc tcgaccacct gtttgaaatg acggtaaaaa
    14521 agcgtcaata aaagcgtgcg gatatgcgcg ccgtcgacgt cggcatcggt catgatgacg
    14581 atcttatgat agcggagctt ttcgagagaa aactcatcgc cgatcgcggt gccgatcgcg
    14641 acaacaagcg cccgaatctc ggtgttcgca agcatcttgt cgagccgcgc ccgttcgaca
    14701 ttcaaaattt ttccgcgcaa cggcagaata gcctgcgtgt ggcggttcct cccctgcttc
    14761 gccgtaccgc cggcggaatc gccttcgacg ataaaaagtt ccgattcccc ggcgtttttc
    14821 gaaatgcagt cggcgagctt ccccggcaag gtgagccctt cgaaggcgcc tttgcgcaaa
    14881 accgtttcgc gcgccgcttt cgccgcaagc cgcgctttcg ccgtcaaaag aacttttgca
    14941 atgattgctt tcgcctcggc cggatgcttt tcgagaaatt ccttcaacgc ttcgttcacg
    15001 accgattccg tcgcggtgcg cgcttcaacc gtccccaggc gattttttgt ctgtccctcg
    15061 aactgcgggt cgccgacctt cacggaaata atggcgacca gcccctcacg catgtcgtca
    15121 ctcgtaagat tttcttcttt ttctttaaga tatccttccg cacgcgcgta ggtattaaga
    15181 gagcgcgtca atgccgaacg gaaacccgtc agatgcgtac cgccgtccgg cgtataaata
    15241 ttattcgcga aactcatttc ctggaccttc gtgtcgtcga tgtacagaaa cgccgcttcg
    15301 acgtttattt tctgctgctc tttctcgacg taaaaaatat cgtcgtggat ccggttcttt
    15361 ttctgggcaa gatacttcac gaacgacagc acgccgcctt cgaaataaaa accgtaaaaa
    15421 atcggcacct ccggccgccg gtcgaaaaaa ttgatgcgga tgcctttcgt aagatacgcc
    15481 tgctggcgca accggtcgaa aatcttcctg tcgttgaatt cgatctcggg aaatatctgc
    15541 gggtcgggag aaaaagtcac tttcgtcccc gtctttttcg acgcgccggt ttttttcact
    15601 ttatagtccg gcttgccgcg cttgtattcc tgtacatacg cgccgccgcc gcgttccact
    15661 tccaccttca tcgtttccga aagcgcattc accaccgaaa cgccgacgcc gtgcaaaccg
    15721 ctcgaaactt tatacgagcc gctctcaaac tttccgccgg catggagcgt ggtcaagacc
    15781 gtttcaagcg ccgatttttt cgtctgggca tggatatcca cgggaatgcc gcggccgtca
    15841 tccgtcacgg aaacccggtt gcccggcaaa agttcgaccg taatattctt tgcatacccc
    15901 gccatcgcct cgtcgatcgc gttatcaacg acctcccaga tcaaatgatg caggccatcc
    15961 aaccccgtcg tgccgatgta cattcccgga cgcttccgta ccggctcaag gccttcaagg
    16021 atgataatat tttcagcgcc gtacgatgca ttgcctttgg cggcgtcttt atcgtccgtt
    16081 tttttagctt ttttttcggg cattttggtt caaaaaaaga tgccataatg gccgcttttt
    16141 atatctctat tgtagcatag tcgagatcag aaatccataa ccgtcgtgtc aggctttcat
    16201 tggttcgcct gcgcttcccc tccgtcctgt catcgtctca tcgtctggcc tgacccgagc
    16261 gacccatgct ttgaatttta attctttctc tttttctttt ttaaagcata gactctcgcc
    16321 tccgcgagaa tgacagacgg gattatcatc gttcgtccga aatcagagcg gctccggtta
    16381 cggatatcgg atcatcttct tacgcagcgc cggtcgtgcc gaaccctcca cgggttttgt
    16441 ttttcatctc gtccgtttcg tcaaactcgg cgcgtgaaaa tgatacgaac atcccctgcg
    16501 ccacccgatc cccgcgcgcg accacaaccg gcccgtcggt aaaattcaaa aacgccgcca
    16561 tgacctcgtc gtcatcgccg caataatcgg gatcgataat gccgacgccg ttcacgggcg
    16621 caagcccctt tttatgcgtc gaacttctcg tggcaagcag gaggaaatgt ccgtgcggcg
    16681 cctcaaccgc gacattcagg ggaacgtagc cgatttttcc gggttcgata accacctttt
    16741 cgcgcgccgc gagatcgaat gccgccgcgc cgtcggtatg gtacttcggc aaaggcaaac
    16801 tcttatcaaa ccgcttaatt tttattttca ttgtaatggt tgtaatgttg catggttctg
    16861 atgtcctggc gcgtttctca ttgtaacact ataacgctag aacgctacca tagctataca
    16921 taaaatttct ttttcatctc ttcctcggcg ccggcctcga actcatcctt cttgatgcgc
    16981 gaccggagcg ccgcttcctt gaggacgtca tcggacatgg cgccgatatc ctcgattctc
    17041 gctataagat attcgcgccc gttcttcgcc ggatcgtcaa gcacttcttc gagcagcgcg
    17101 cttaaaagcc accccacgcg cggcccgggc gcgatgcgta aaattttcat cacgtcctcg
    17161 ccgttcacgt tgagcatctt gggcgataaa ggatcgtgtt tcactttttc gatcataaaa
    17221 agcaggtgcc gcaatttata cgggatcgcc ttttttacgc ctgaaccgat ccggtctgcc
    17281 tcgcgcacct tcaggagatc gtcgatattt tccggcccca cgcgcacgag gaaccgccgc
    17341 acgccggcat cggaaatttc gccgacgtta taatagaaca tgtgccgccg cacgagatgc
    17401 gcgaccttct ctatgaattc cttcggaaac cggagccggt cgagcgcccg cgccgccatc
    17461 ttcgcgctga tatactcgtg ctgataaaaa gtggcatcgg cgcccgtgcc gccttttgcg
    17521 cgcgttttgc cgatgtcatg caaaagcgac gccatgcgca cctcaagcga ataatcctgc
    17581 tttgccgcgt aatcgagcga acggacattg tgctcaaaaa tcgtataaac atgatgcttg
    17641 ttctggtcga taccgatccc ctcgcgaagt tccgggagga cgtactctaa aagattgagt
    17701 tcttcaagaa aaagaattcc ttttgccgcg cgtgacgccg tcatcatttt cacaagctcg
    17761 tcccgcacgc gttctttcgc gataaccgca agttcgcgcg aacatttctc tatcgcgcgg
    17821 cgggtgttca attctatttc gaaatcaagt tctgtcgcaa accgcacggc gcgcatcagg
    17881 cggagcgcat cttcggaaaa ccggtcttcc ggcgccccga cggtgcggat gattttgttt
    17941 ttaagatcgg cgcgcccgcc aaaagggtcg atgaggccgg aaatggtaat ggccggatcg
    18001 ctttgcctgt cggatcgctt tgcctgttct gatgatttat ccaccattac gacattttca
    18061 gcattgagaa catgtacaac atcaagcgcc atcgcgttga ccgtgaaatc cctgcgcgag
    18121 aggtcttctt cgatggtttt cgcaaatttc acttcgtcgg gatgccgctg gtcggtgtac
    18181 tttccctcga tgcgatacgt ggttatctct attactttca acgccggatc gtccgagccg
    18241 gttttcacgc ccaccgtgcc gaagtcattt tcatacaccg tcgccggatt gtccgcaggc
    18301 gcgccttcga aaccggagaa tattttttgg atatcgtccg gcttcgcgtt cgtcgcgata
    18361 tcccaatctt tcggttcaat gcgcccgccg cgcgcggcgc caagtaaaag atcgcgcacg
    18421 catccgccca caagatgcgc ctcgaatccg gcgtcccgta aaacccgcgc aaccgaccgc
    18481 acttcttccg gcacgtctat tttcatggct ttaagtgtag cgcatgcggc gctattccga
    18541 aatcccggca tgcctcggga ttttttgcat aaaaatacga acggcatata ataaatggca
    18601 tggacatcca aaaaaataag ggcgttacgt ggatcaatat cgtggcgccg acggcaaaag
    18661 acatcgcgtg gctgaaaaaa gaattcgcgc tccatcccgt gatcgccgaa gaattgagta
    18721 atccttccgt ccgcggtcgc attcaaatat tcaaaagcta tctttttttt atcggctatg
    18781 tgcccgtcta taacaacaaa gacgaaacat cgctccggat ggaaattgat tttgtcatca
    18841 cgaaggacac ggtcgtcacg ctgcaatacg aaccgttcaa agaagtattc cagaactttc
    18901 gtctcgacgg cgaagaatcc tcgcttgaac tgatgttcaa gattatggag cacatcaccg
    18961 ttttcgagga acgccagctc cgccacatgc gggaaaaggt ggaagccatc ggcacggaac
    19021 tcttcaaaaa taaagaggat gaaattttca gaaaactcac ctatctgaaa cgcgacgctt
    19081 ccgaataccg catcatcgtg aaactccagg aacccgccct ccgcgccttg ctttcaaaag
    19141 gcatgaagtt ctggggcggc gacgcggaaa tatacctgaa cgacatcctc ggtaatcatg
    19201 ccaatatcgt gaatcagctc gaagattgcc gcgaagccat cgccgatttc gaagacacca
    19261 ataaccagct tttgaacgcg aaggtgaacc gcaccatgaa aacgctcacc gtgctttcat
    19321 tcgtcacctt ccctttcatg ctcatcgcca cgctgttcag tataaacgca aaggatacgc
    19381 ctctcatcaa cagcgcggga ggattctgga tcatcgtctg cgccatggcg gcgggagtca
    19441 ttgcgcttgg tatttatttt aagaagagga attggtttta gtgttttaaa cgcgccattc
    19501 tgtcatcccg aaaaaaacac gaatcttcaa acgttcatcc ggaagccacg ccctctttgc
    19561 cactatggat cccggattgc gggcaaagaa gcccttgtcc accggaattt tccagaagaa
    19621 gatttcttga aaaatcagga aactttcccg gaatggcaag aatgaaaata aaaaaactta
    19681 acactgcccc tgccatcatg ctcacgcttt acaatacgct ctcgaaggaa aaagaagctc
    19741 ttccggacgc tgaaagaata aaactgttcg tgtgcggccc tacggtgtac gattatgcgc
    19801 acatcgggca cgcgcggacg tatatttttt ttgatttttt cgcgaaatac ctgcgatcgc
    19861 agggatatga cgttttttac ctccagaata tcaccgacat cgacgacaaa atcatcaagc
    19921 gcgccgtcga agaaaataag cagccgctcg agttcgcaaa attctttacg caggcctata
    19981 aggacgacat gcgatccctc ggcattgaaa tgacgtcaga taaaaacgag gcgaagcgcg
    20041 aagggaaaat actgtacgcg gccgccacgg cgttcatgcc ggaaataatc gatcaagtgc
    20101 aacggcttat cgaaaaagga tatgcctaca gaatagacgg cgacggctgg tattttgata
    20161 tttcgaaaga tgcggattac gggaaactct cggggagaaa cacgctgcaa gcggaagatg
    20221 ccgtttcgcg cattgacgaa gcggtcggaa aacggaataa gggcgatttc tgcctttgga
    20281 aattttcaaa atccggagag ccctcatgga aaagcggtat tggcgacgga cggccgggct
    20341 ggcacatcga ggacaccgcc atctcggaaa agtttttcgg cccgcaatac gatatccacg
    20401 gcggcggcat cgatctaaaa ttcccccatc atgaagccga gatcgcccag caggaatcgg
    20461 catccggcaa aaaaccgttc gtaaaaatat ggatgcacac cggcgcgctg cttgtcgacg
    20521 ggaaaaagat gtcgaaaagc ctcggcaatt ttataacgat acgcgcattc atggaaaaat
    20581 atccgccgga ggttttgcga tggatgacgc tcatgcataa ctatcgcatg ccgttagatt
    20641 acaccgaaaa aatggctgaa gatgcaagga ccaacctgaa cacgattcga agatttttat
    20701 ggaaattaga attcaattta aaacaggaaa ctttcaaaac aaaaagtgtc ttgttggatg
    20761 attacgatta ttcgaaattc gatgcgttca ttgacgacga catgaatact ccggaagcat
    20821 taggttttgt ttttaaggcg ttgaacgacg ccaatgaacg cctttcccag ctcgatgaaa
    20881 acgccttgcg tagagcgatc gatttcatta aaaaagtatt aaaactgttc ggcgtgaacg
    20941 ctgattatcc cgaaattcct caagaaataa atgaacttgc cgaagaaatg gagcaatgca
    21001 gagttaataa acagtttgag cactccaatc gcttgcgtga aaaaataaat gcattaggat
    21061 ataaagtaga aaatacaaat acgccgttcg aaccgtttat atatccggga tgaattgatt
    21121 gatgccaaac atcgctcacg ttcattgctt ttcctgttgt cctcccgaag aaagaggatg
    21181 acagaaatgg ctgcttttat cattcataat ttaccattca taattattcc tccatggcaa
    21241 cattaaaaaa atcccaactg aaaatcccgc cgcagaacct cgaagccgaa caggcggttc
    21301 tgggtgccat ccttatcgac gagaatgcga ttttccgcgc cgcggatctt ttgatccctt
    21361 ctgatttcta tgcgccggcg cacgaaacca tttacggcgc catccttgaa ctatattcga
    21421 aacatcagcc gatcgacgtc ctgagcctga ccaaccggct taaagaaaag gaacaattaa
    21481 aagatatcgg cggctcggcg tatctcgccg atctcacgag cgccatcgcg accgcatcgc
    21541 acgtggagca ttacgcaaag atcgtaaaag ataaaaaagc attacgggac gtcgtgaaag
    21601 cctctgccga aatcacggaa gatgtttttg agtcgtcgcg ggacgttgac gacattctgg
    21661 acgatatcga acaaaagatc ctcgccatct cgcaaaaatc gcttccgcag aattttgtcg
    21721 cgctcaaaga agaactcgaa accgccctaa aacgcatcga aaatctccat agcggcggcg
    21781 caaagctccg cggcatcacg accggcttta cgcaactcga caacctcctt tcaggacttc
    21841 agaaatcgga gctcattgtc ctcggcggac ggccgtcgct cggaaaaaca tctttcgccc
    21901 tcgacatcgc gcggcacgcg gcgcgcgcgg gacacacggt cggcgttttt tcgcttgaaa
    21961 tgtcgcgcga agaggtcatc gatcgcgtca tttcggcgga atcgacggtt cctctgtggc
    22021 gcatcaggac cggccgcatc acggacgata tggatttcac gatgatccag ggcgccatgg
    22081 acaagctctc aaaggtaaaa ttctttattg acgattcccc caccctgaat atcctccaga
    22141 tgcgctcgat ggcgcggcgg cttcaggttg aacacgggct cgatcttctc gttgtcgatt
    22201 atctccagct catcaaaccg cgcacgaatt ccgacaacgt ggtccagcag gttacggaaa
    22261 tttcccgcgg attaaagtcg ctctcccgcg agctcaaggt gccggttctc gcgctctccc
    22321 agctctcgcg cggcgtggaa caacgcgacc acaaaatgcc gcggctttcc gatctccgcg
    22381 aatcgggttc catcgagcag gacgcggacg tcgttatgtt tatctaccgt aaagaccgcg
    22441 aacatggcgt cgcgatcgcg cctgaagaag aaggcgtcgc gcagattctc gtacaaaaac
    22501 ataggaacgg tccgacaggc gaggtcgacc tgcgtttcga cgcggaacac gcaagtttca
    22561 agaatatcga ccgtcggcac gaggacaccg gaagcgacgg agggttcgat atcagtttat
    22621 aaacgaagta atgtaaatta ataaggatgt cctcttctct gtcgtcctca cggaggcgag
    22681 ggtccatgct ttaagaattt cagaaaagat tggaagcata gatccactcc ttcgaaggga
    22741 cgacagaaat cttttctatg agggcgataa aatattcccg tctctacaga aataaatata
    22801 tcattagtat ccatgaaact ccccgatccc ataaaaaacg tcgttgacgg gctttcgcgg
    22861 cttccttcaa tcggcccgcg ccaggcaatc cggctcgcat tttatctcgt gaacgagaac
    22921 aaagaggaca tccacgatct cgccgtcagc atcaataatc tccgcacgct caagatctgc
    22981 gaacgctgtt tttttatcca ccaaaataaa ggcaatctct gcgacatctg cgcgaatccg
    23041 acccgccggc agggcgtcat tatgattatt gaaaaagaaa ccgaccttat ttcgctcgag
    23101 aataccggaa aattcaccgg ccgctattgt atcgtgggac ccatacccaa aaccggactg
    23161 cttgaggact ggcaaaaaaa gcgcatgcag agcctgaagg attttatagg aaaaaacgct
    23221 accgagggcg ggctcggcgg aaaaaccgac gagattatct tcggcttcaa tccgaccgcg
    23281 ctcggcgacc tgcaggcatc catcctgatg aaagaactcg ccccgctcgc gaaaaaaaca
    23341 agccgcctcg gccgcggcct tcccaccggc ggcgaaatag aattcgccga cgacgaaacg
    23401 ctcggcagcg cgctggaccg gagatcgtaa agagaaattc ccgaaaacag aactcccgcc
    23461 tcagcaatgc gaatgccgac gccgccttcg tttcactttc ctttttgtgg accaaccgag
    23521 aattgaaccc ggacctcagc aatgcgaatg ctgacaccgc cctgaatttc attcctttat
    23581 ttgtggaccc agggagaatc gaactcccgc ctcagcaatg cgaatgccgc gtaataccac
    23641 tttactatgg gcccatgctt atctattgta tgaaattcag ggcgggataa caaattgatt
    23701 accgcgcctt tttggcgcga gtccatttta ctattggccc ttatgggaac tgaataaata
    23761 atagtaagtg aaacgagagc ggacgacaat tgattcgctc gcttttttac gaagctcact
    23821 ttactatgag tccgatattg tacagtataa ggattaaaac ggaaaattca agaagaaaat
    23881 aatacggatt ccatgttctc cataaataaa tcaaatcgat ttacgggaga tgatattccg
    23941 cataaaatgc gacagagggt cgaagggatt acgtttggca ttgagaaaaa tgtaaaga
//
LOCUS       DAIDDT010000005        21334 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_5_length_21334_cov_6.661288, whole genome shotgun sequence.
ACCESSION   DAIDDT010000005 DAIDDT010000000
VERSION     DAIDDT010000005.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21334)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21334)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21334
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5_length_21334_cov_6.661288"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    19344..19353
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5161 a         5705 c         4745 g         5713 t
ORIGIN      
        1 cttttcttgc ggcttccgcc attttttccc tttcctggtc atgttttaaa taatattcta
       61 ttttactttt cagttcttcg gcgtcgttaa aatacgcgac cgaatccccg aataattgtc
      121 gcgacaaagg gatatcctcg gcgacctgga atgtccccga cagggctatg tcgaatatcc
      181 tctgcgtagg gccggtatac ggttcctttg aaagccatag cgtcccgatg gcaatcttcg
      241 ccccgctata gattttattc aatgccggag ttgaggccac gccgcagtaa ttgcttttca
      301 gccaaggata cttcgtaaac ccatcctccc agccatagcc gtatattttt atcttaaatt
      361 ctttcaaaga atccatcaca tcggcgcgcg ccggaatata ctttcccaca aaaacgatat
      421 cggcggtttt tggccggttc ggcaaaggga agaaaatcgt tgcatccgac gcaagcggca
      481 aaagccgcac ccgttccttg tttttctttt cccgcaacgc gtcaatccac gtgccgttgc
      541 aagccataaa aacggaatcg aaataatcga acagcggctc ccatgaagca tcccgtacgg
      601 ttgattcgcc aagccagagc actaacgtaa tatttctctt tttcaagaat ttcaaaaccc
      661 atgccgatcc gggaacccag atatacacgc agatatggtt cgcatgcttt ttgcttaaaa
      721 aaattttcag gcacaactcc gcgtccgaca aaaacctcct tgccttcttc aaaaacggaa
      781 ataaaaaagg gaaattcttt tgcacataag attttgcccg ttcaaaaaaa gaaaccagcg
      841 cgtcacggtt gccgcccatg gcggccggaa acgcatttac gtaaataata tcggcggcaa
      901 tcccaagccg gcggcacgcc aaataaaaat tcttcgccca atccccgtac caagcggatc
      961 cgatgacggt cactttgaga tcgttctttt tcatattcat atgcgcggtg cgtccccgta
     1021 ttgatcaaac gggatcaata agttttctcc gatttttttt acgattgccg catcaagaac
     1081 agcgctcccg ccggatttct ttttctccgt aatcgctttt cttatagcat ggataagccg
     1141 ctcgtaatcc ccgtccggca cgacaaatct tttgccaaaa agatacagcg aaccgtcgtt
     1201 ctcgagataa aaaaggcata accgttttgc cgatactttt ttgtattccg cgaaccgccc
     1261 gcggagatcc cgggattgca tccatgcggg aaacatcagc cgcaaggcct caagaaacgt
     1321 gattttattt tcaatccaga cttcccatgc catagagcgg tttgcaacgc tggaatccca
     1381 ggcataaaac tgtttctttt cgaaaaaatg ggcaaaatcc tctctcatag cgtcttcaat
     1441 gaccgcgctc ctttgcgctc ctctcggtta tccgtataat gtcacggttt acctcgacat
     1501 ataaattgag tccggccgcc atttcgtata ttttatccat agaaggatag gttccgtccg
     1561 atccgaagga atgagcgtca tctatcaaga taacgctccc gggtttccat ttcgcaaaac
     1621 acgttttcag ttcttcggtg atgggacttt ccttatcggc gccggcagtc cgttcgccgg
     1681 tttccatttc gcaataatgg gcgtcaagcc aatagatgac cggaacatcg agtgaaagct
     1741 ttttcagaaa caaagaactg tcatcatgaa aaagatgaat tttgggatca cggaaattat
     1801 ggagcgcttt ttcgtaaaga acatcgtcaa gctccaccga ataaagatgc ttaaaatatt
     1861 tccggagccg ccccagtgta tacccaagat tggtgcccgt ctctacgaaa gtagagagtt
     1921 taaacttctt ggcatatctt ttaatggtgg atattttata taacgggtgc cccttggggc
     1981 ggataaaacg agtaatatac gcctttcctt tttggatagg cctgtacaat ggatgggaac
     2041 gaatgcgatc ttttatgctt tttatattca tgatttttta tctgattatt ttcaacagta
     2101 tactatgaat ttaatattct tgaaatcttc cttatgattt ttttcggcat cggcatagga
     2161 ccgcataata tatggaatcg aatatcggca tcccggcaca gttccacttt ttcggaatga
     2221 gtaaccggca gaatagggga tgtcaaaata ctcgcgcagt tgctatcaat gtcctgcgta
     2281 tgcgtcgcat catccccaaa tccgatatta ctgacaagat tcacgttcgg aataatcgac
     2341 aatccgcgct tgttccagat ggaaaagagc aattgggggt cccatgtggt gatcgcctta
     2401 tcacccttca cgcgcttaaa atatttttcc caatagttcc tttgcggctc ataatgaaaa
     2461 taatattcca aagcattttt gacgtcctct tgcttcacgc tgtccaatgc aaaatcatag
     2521 atgtcccatg ttcttttcca tattgcatac ccccacaaat gagggaacgc ggaaaaataa
     2581 tacgaaccgg cgccccattt tttgccgccc tggaaattat tgccaccgac gagcgccacc
     2641 tcttcgttgt cccgatagcg ctcaagcatt gccgcgcaat aagggaaaaa atcaggatga
     2701 ggcatgcaat catcctctaa aatgatccct tcctcgacat tatcgaaaaa ccatgagatc
     2761 cccgaaggaa cccctttcgc gcagccaagg ttcacgtcgg aatatttccg atgtacctcg
     2821 cattcccaat ctatctgttc ggttatcttc cgcgcctctt cgcatttctc tttttcgcca
     2881 accttccctt cccgcggtcc gtcggcgacc acgaacagtt tccgcggcct gacttttctt
     2941 atctcgtcaa aaacaacccg cgtggcgccg ggacggttga aaattatgaa aaggacaggg
     3001 acgctgaatt ccggcgtgtt ttgcatgaag attttattca cgtcaataag cgataaggta
     3061 ttcttcaaag agcggggtta atggattggt gtgcactatt tgaaatttat aatccgggtt
     3121 tatttttttg atatattccg gaatctcaaa aaaatccctt cccgagtggt ataacgcaat
     3181 agaaagaacc ggcttatctt cggagagaac ccgggcggcg ccttttacgg catcaaactc
     3241 agccccttcg atatccatct ttatcaagcc gatcctcttg cgattgtttt gctctttcag
     3301 gtcatctatg gaaaccaacc gcactttttt atcgccctta tcgccgcttt cggaaagaaa
     3361 cgccccgatc ccttcgccgc gcaaaaacgc ttcccctgac ttatccgaaa cgcctgcatt
     3421 aataacggtg atgttcctca tatcgttcag tttaatattt tcttccaaaa tgccgatatt
     3481 tttttcttcg ggttctacgg caaggatggc ggcagggcta tatttctgga gcgcgtagca
     3541 actatccccg ataaatgcgc cgcaatcaat aaaaagcgaa cccttgatcc cttcgcgggc
     3601 ttcggcgggg ataagatcca tcccgcaatt atttgccaaa acgccatatt cgaaccccgg
     3661 cactcccgtc ctgaaccccc gataatcgta catcccctct ttctttctga tattatcgcg
     3721 atgcgccttt tgtgcgcgcc ggaatagggc ataggcatcg cccggaaaaa catttttcca
     3781 gggaatataa tcttgataag caaattgaaa atgggccgta aggaaccgct cgatctcttt
     3841 gtgcgaatct tcgtcaaaat aattcatatg cagggaaggc cggcgctgaa gaatatagtc
     3901 aacataatcg atcgcaaatc tcttttctcc cccggcaacg ggatatttaa ggagggtctt
     3961 gcagtgtaat accgacttgg ctgtctgctt ctgttgatac gtcaaccggt ccgcgataaa
     4021 acgccaccag aaagaaaggt cattcttctt tttgaattct gcgtacccgg cgctttccaa
     4081 gcccaatgcg ctgccggcat attctttcgc aagccaatct ttataaaaaa ctacgctatc
     4141 catgtttaaa aggataatcg ttcaaaaaaa taaaaacaag taaaacgccc gccgcaagaa
     4201 ttgcgacggg cgcatagtaa aaccaatacc ttggctctta tcatttcgtc gcgataggag
     4261 cttctgtcga ttgcacgccg tagagcagat gatcataaag ccagtgacgg gtttcatcac
     4321 cgagccgctg aaaaatgatt tcatcttcac ccaggaagat accctcatcc agcattttct
     4381 ttgcaaatcg gccgcccgac ggcaggaaag taaagtaatg cgggtttttc gcgatcggca
     4441 gcggtaattg atcaggtccg gaatacccca tggcatgaga aagttcaacc tcccatcgta
     4501 cggcttgatc gccatatccg acagctgcga gatgccgctt tgtaagatgt cccagcgtgg
     4561 cccacgtcca ttcatccttg ggaagatata tgaacttccg tgcgagcgta tggccttccg
     4621 gcagtttcgc aagccagttg aaattgcgct ggagaataac catctgatcg caataccgct
     4681 cattctccgt aaaaccaaga tgacaaatcg tcgcactaat accgtcgaac aacggattat
     4741 gactctgatt gcggacaagc gaccgaacgg attgctcaat tgaagcaggc cctccgtccg
     4801 catgggacaa aacactccga ttgaaaaacg tatcgtccaa gtcatcgttg cccccgtggt
     4861 agaaaccata tttcgaagtg atggcgccca tctgcgtccc tccataggga gaaagaatgg
     4921 atgtccacgc aatctccgga ttaagacgga cattcagctc gagcgtctgg atatccgtct
     4981 cgatatcact gaaaggcact ccaagaatct gttccgtgcg aagcgcgaat ccccggtctt
     5041 taattttttt gataccgtca acaatgaggt catccggcat cccgcgcgag agaacaaaac
     5101 gccgaagaaa atcatttcct gactcaatcg ccagggtaat gcccgtacat ccgccttcac
     5161 gaaaaaggtc tatccgccgc tcgtcacgcg taagttcgag acgaatctgg caatgccatg
     5221 gaatcccgac tttctccttc cactcgcgaa caaattccgc gagccaggca atgttgagcc
     5281 cgaagttgtc atcctgaaaa taaatcatcg ataatggcca gcgatttttg atcgcgagcg
     5341 cctcattaat gatttgcgga atcggccgaa ggttcagcct aaatccgcca tacatcttat
     5401 tgtacgcaag cgcgtagcaa taagagcacc gaaagggaca tccaagagag catatgatgc
     5461 ttttgatcgg gctttccgca agatgcggat aagcattgta agccagatcc ctgcccggaa
     5521 ccggaaaccc ttccgccatt cgcgtttcgt cgaaatggat tccaggcaca agctcgcctt
     5581 tgagtaaacg ccggaagtta cggaatgcct cgcccttaat gacccattga gcatgcttcg
     5641 tgcattcagc cgaaaaatac gtcgcatgcg ggccgccgat tacaacgggc actcccatct
     5701 tccgaacttc atcgcaagcc atgaaagatt gtcgatgata tccggtccaa atcgagaacg
     5761 ccacgatgtt cggcctgaaa aacctgacct cgtgatacaa cggttgaaaa tcaaagtcat
     5821 ccacgagaac gactttcacc tcccatccaa gctgctcggc aagtcccact aaatgaagaa
     5881 tgcccaacgg ctcaatggga gagtttttcc ctactataac gatcttattc acggaacacc
     5941 tccttttatt ggtgataaaa tgtgtcaaag aactacattt aataataaaa cattcagaaa
     6001 atattgcaac ctcgccatta ttcaaagata cggaaagaaa tgcccgtaag gctatgtgcc
     6061 gcaataataa aacttctagc aaaacacata aaaagagcag gaagactttc ttctcatata
     6121 aaaatcctgt tcttctttat ataggcaatg tccataattc tctacatcct tatccatcct
     6181 tccggaacga tgtctatctg gtcccctttc ggggacgggt tccatttttc tggcgcaacc
     6241 actattttat cgggattgcc gttcagccat gcgccccacc aggaaaacgc gctgttcacg
     6301 atttgaatat ttttattcac tgctttccgc tgccacgatg cgcttcgcgt attcctcgta
     6361 ccacggcagc atctcctgga tggcgcagaa aaacatctca taatcttctt caatgtcata
     6421 gtcatggaca agcacattcc gcacggcgac gctccgcgcg caccgcgcgg caagatcgtc
     6481 cgaaatgata cctaacggaa ctaatttgat aaatgaatcg cgataggtat caggcgtttt
     6541 cttcgtcttc gcgataagga tcttcgcgtt gatatcgctc gcaatctcga ccatcagctg
     6601 gaaatttctt tcggaagcgc ggatcagcgc cgtatccgcc gcaaactcgg caaacggccg
     6661 cgcggtccac gcgcgaagtt cgacgatcaa tgcgccgatc tgctccaatt tcttctggat
     6721 aatttcattg ctcataggta ttgttccgcg agcatcgcgc gccgcgcgcg gcggaatcgc
     6781 gccgtatcct gataattttt ccatgcgcga aagatgaaat cgtaaaaatc atccgcatcg
     6841 ccggaaaaca acgagccgcc ggtcgctatt tcgtattgca acaaaggcgg tacggaaaaa
     6901 gcgtcaacga ggtcgatgtt atcttcggaa acgccgagta atcgcgccgc ttgttttata
     6961 tattcctctt tttcggcaaa cgaaaacgca tgcgacgcaa aaacggcgac atcgtagtcc
     7021 gagttttccg tcgcatgcgc ggacgcgcgc gaaccgaata aaataaaaag cggctgatgc
     7081 atctttttat tataacaatt ctctacatcc ttatccatcc ttccggaatg atgtctgcct
     7141 ggtccccttt cggggacgta ttccattgtt ccggcgcgac cactatctta tcgggatgcg
     7201 cgttcagcca tgcgccccac caggaaaacg tgctattcgc gataatattg tgcttgcaca
     7261 gcgacatcag ctgaagatga aatgccgctt tcggaccggc gcttgcgtcg tcgagatacg
     7321 taaccgaata gggaattttt atattctcgc ggcaccatgc gctgtcatcc gaaaaaataa
     7381 agaaatgcgg cgcggaaaca tggtccgcca tataagcgac cgcgcgggaa taatacgaaa
     7441 gatcggtgcc gcccgttatc tttttcacgc tttcgaattt tacgtaatcg ccgcggcgca
     7501 cgtggagcga aacggaattc gaatttctaa ttttttccgc aaccgccgcc gcttcgcccg
     7561 caagctcacg cttgaaacgg aatgcgcgcc ggacatcgtc cgcattgtcc gcaaaatatt
     7621 tttcgctctg ccaataaccc cataaaagaa aattgccttg cgcgcgaagg acttccggat
     7681 cgaagtgatg ctcgttcttt tcttttatta tctttcgtcc gaaaaatatt tcccgaattt
     7741 tcataaacgc aatatcaagg ccgagccaaa gcccgggaac cggaaatttt tcagaaagtt
     7801 ttgacagggc ggtaaaatgt ggtttattct ccttgatttc gtcggacgaa agcgtaaaaa
     7861 tatcgagatc gaacacgcgg tacgtaaatt ccttgcgcgg aaaacggtcg ttcaaaaaag
     7921 tcgtatcgag aagaagctgg gcattatttt ttttcgcgag atgcagcccc agcgcatact
     7981 ggaacatctg attgcccaat ccgccgaaca ggaaaacggt tgttgttttt tgagccataa
     8041 tggtcaatac cgtcactctt taaaaacaga tattcttaag cccgtattta gacactaaaa
     8101 aaaccttcta aatctttctt tgtaaatccc gcctcgcgga taatagcaaa aagcgtacca
     8161 atcggcagat caccaggatg ccgcggcacg accgcgcgac gttttgtgac cggttcataa
     8221 aatatgaaat ggcttcctgc cgcataatcc aattaaaatc ctctcgcttt aagaaagatg
     8281 atgaccctcc gcgccgtaag tttaggcaat tttgacataa tcaaattcaa cggaagcgat
     8341 aaaagtttcc gcatcggtcg gtgcctgttc gccgtgcttc cgcaagctag aaagataagc
     8401 gctgattgct tcgcgcgcca tttttttcgc cccatccaat gtccgcccgt acgtcacaca
     8461 ccctggtaat gccggcaccg taacggtaaa accgccttcc ggttccgacc gaaaaagaag
     8521 attgtagtga agctgcttca tattaaataa agtatattgg ttgaaaatat aagtcaattt
     8581 ttacggtcta ccacatacca atcaatcgtc tttttaagcc ccgtttcgaa atccgttttc
     8641 gccttgaatc cgaattcttt ttccgcgcgg gacacatcaa gcgaacgccg cggctggccg
     8701 tccggcttcg tcgcgtccca cctgatctcg ccgtgataat ccatcagacg gcatatcaat
     8761 tcggtgagat cttttatcga gatttccatc cccgaaccga tattcaccgg ctccggcttg
     8821 tcataacgct ccgttgcaag cacaatccct tccgccgcat cgtcgacgta aagaaattca
     8881 cggcttgcgc ttcccgttcc ccacgcttcg ataaacggcg catgcgcgat cttggcatcg
     8941 tacacttttt tgataagcgc cgggatgacg tgcgagcttt tcggatcgaa attatccccg
     9001 ggtccgtaca aattcaccgg caaaagatag atggcgtcaa agttgtattc ctgccggtat
     9061 gcctgcgcct gcacaagcag catttttttt gccaggccgt acggcgcgtt ggtctcttcg
     9121 ggatatccga cccagagatc gtcttccttg aacgggatgg gagtgaactt cggatatgcg
     9181 cacacagtgc cgagagcgac gaattttttc acgcccacga gacgcgccgc ttccactaac
     9241 tgcacgccca tcatcaaatt ttcataaaaa aaacttcctg gatgctcgcg gttcgccccg
     9301 atgccgccga cgaccgccgc aagatggata ataatgtcct gtccgcggac tgccgcttcg
     9361 caattttccc atttccggat atccagctcg gacgaccgcg gcacggaaat attttcccgc
     9421 ggaacgccgc gcgccacaag cgcgtccacg acgcggcctc ccaaaaaacc ggctccgccc
     9481 gtcacaagaa tttttttgtt ttgtaaatca attatcatgg aagtttccgc taaaattatt
     9541 tctccgcgtt tctcaattcc ttgcgcgcca gggaaagatc gtaagccgcc atttcgcgga
     9601 cgagatcgtt aaaagaggtc ttcggcgtcc atccgagttt catctttgcc cttgtggcgt
     9661 cgccgtgaag cgaatcgacc tccgccggcc tgaaataacg cgcatcgacc gcgataatct
     9721 ctttccctgt tttcatgtca acgccgacct caccctcgcc cttccctttc catgccaacg
     9781 cgatgccaaa ctctttggaa acagcatcgc aaaattcacg gacggaatgc tgttcgccgg
     9841 tcgcaataac gcaatcatcg ggatctttct gctggagcat cagccacatc gcttcgacaa
     9901 aatcccgcgc atggccccaa tcgcgcttcg cgtccagatt gcccagaaaa agcttatcct
     9961 gcgatccgac gctgatgcgc gccaagccgc gcgttatctt gcgcgtaacg aacgtttctc
    10021 ccctgcgcgg gctttcgtga ttgaataaaa tgccgttcgc gacgaacatt ccgtatgcct
    10081 cgcggtagtt cacgccgatc caatacgcat aaagtttcgc gacgccatac ggactgcgcg
    10141 gatggaacgg cgatgtttcg ttttgcggcg gcggcgtcga gccgaacagt tccgaggtgg
    10201 acgcctggta tattttcgtt ttcttttcca atccaaggat acgcaccgct tcaagtatgc
    10261 gaagcatgcc gagcgcgtcc gattgcgcgg tatattccgg cgtttcgaaa gaaactttca
    10321 cgtggctttg cgcggcaaga ttatatattt catccggctg taccgtctgg atgatggaaa
    10381 taatattggt cgcatcggtc atgtctccgt aatgaagcac aagacgccga tccttttcgt
    10441 ggggatcctg gtaaaggtgt tcaatgcggt cggtgttaaa actggaagaa cgcctcacga
    10501 ttccgtgcac gtcgtatcct ttttcgagca gcagttcagc aagatagctt ccgtcctgcc
    10561 ccgtcacgcc ggttataagc gcttttttct gcgccgcttc gcccgccgtt tcttgttttt
    10621 tattttttgt ttccatagct gtattttatc acaaagaaaa atttttcata gtcccgttga
    10681 acagattagc tttgtaacct tcttcgtcgt cctcgccctg tatcgtgtgg tataaggtga
    10741 actccggctc gccctatgga gcggcggctt cgcctgctcc gcggggcgaa gaaaataatt
    10801 ttcgagggtg gccagattcg ttctattgcc gcaaaaaaag cgtatactaa aaaaatccca
    10861 tgatcatcct tctcacgggc aacggcaaag gcaaaacgac cgcggcgatc gggcaggcgg
    10921 tgcgggtttt gggacgcgga aaatcggcgt tgattatcca gtttatcaaa ggtagctgga
    10981 aatccggcga agacgagttt gcggcggcgg caaaaattcc cgacagccga tttgtgatcc
    11041 gaaaaatggg acttggtttt gtgggaatag cgggcgatgc attgccgatc gagcaacata
    11101 aagaggccgc gcaaaaagcg ctcgatgcgt tcattgaaga aaagaaaaca ggcctctggc
    11161 atttgatcgt gctggacgag atcaatgtcg ccgttgcgct cggccttctt gcgccgaaaa
    11221 aagttttgga cgcgataaag gatttccccg aagacaagat cctgatgctc accggccgcg
    11281 gcgcgccgca ggaattcatc gatgccgccg atatcgcaac ggaaatgaaa gaaataaaac
    11341 atccgttcca aaacggtaaa gaaggaagag agatggtgga attttaatac tgctgttttc
    11401 gtcttctcga aagagacgtc tcccttctgt cgtccctaca atccttctgt cgtcctcgcg
    11461 aaggcgagga tccatgcttc ccgtccgacg gtgtcgtccc atcgaagaag gggatccatg
    11521 cttcccgtca taaatccgtg agttttaaat cttgaatcgg aaaattcaga aagcgtggat
    11581 ctccgccgga gtttatcccg agttttcgag gggagaggac gacagaaaag aaagaaagcg
    11641 gacaaaacac aaaattttta cgccgctatc ggttttatct tgaatggttt cagaagatta
    11701 tagaaatcgt cgtgctcgag cgtttctttt tccataagta cggtcgcgat tttcgtgagc
    11761 gcgggcttct gcgccttcag aatcttttgc gccatctcga aagcgtgcat caggaacgcg
    11821 cgcacctgct cgtcgatctc cgccgcaacc ttctccgaat aattcttttc cgtggaaatc
    11881 tcgcgcccga ggaacagcag ttcttcggtc ttgccgaatg tcatcggccc catatcgctc
    11941 atgccgtaac gggtaacaag ctggcgggcg agattggtcg cctctttcag gtcgttcgac
    12001 gcgccggtag agacatcgcc gaacgtcagc ttttccgaag cgtagccgcc catcatcatc
    12061 gcgagatcgg ccaaaaattg ctgccgcgtt ttcaggtgct gatcttccat cggaagcttc
    12121 atcgtatagc cgcccgcgaa cccgcggctc acaatcgata ctttctggac gggatccgcg
    12181 tctttcaccg ccgccgcgac gagcgcatgg ccggcttcgt gatacgccgt gatctctttt
    12241 tctttcggcg agatcacgcg gcttttccgt tccggcccga ggattatttt ttcagacgcc
    12301 tcgaaaagtt cgtcctgccc cgccgctttc ttgttgcgcc gcgcggcgat gatcgccgcc
    12361 tcattcataa ggttcgcaag atcggcaccg gaaaatccag gtgtccgcac cgcaactttc
    12421 cgcaggtcaa cgtctttctc aagtattttt ccctgcgcgt gaatcttcag aatcgcttcg
    12481 cggtcattga gatcgggaag gtcgaggatc acgcggcggt cgaaacgccc cgggcgcagc
    12541 aacgccggat cgagaatgtc gggacggttc gtcgcggcaa gaatgattac acgcgtatcg
    12601 cggtcgaacc cgtccatctc gacaaggatc tgattcaacg tctgctcgcg ttcgtcattg
    12661 ccgccgccca atcccgcgcc gcgctggcgt cccaccgcat cgatctcgtc aatgaataaa
    12721 atagcgggcg ccgcgcgctt cgccgtcgcg aaagcgtcac gcgtgcgcga tgcgccgaca
    12781 ccgacaaaca tctcgacaaa ctccgatgcg gaaatgtaaa aaaacgggac attgctctcc
    12841 cccgcgaccg cgcgggcaac aagcgtcttt ccggttcccg gcggccccat aagcagaata
    12901 ccgcgcggga tgcgcgcgcc aagatccatg aattttttcg gatttttcaa aaaatcaaca
    12961 acttcaataa gctcctcttt cgcctctttg aggccggcga cgtccgtgaa catcacttta
    13021 tccttgaaat tcgaaagatg aagattggaa cgcccgaaat tgaacgcctg attcgcgccc
    13081 gcgcgcgacg agcggaacat catccaaaaa ataaaaccga taataagcaa cgggagcacg
    13141 gtcgggatga ggagattcat ccagaatgcc cacccggact gattctggac ctgaaaatca
    13201 acgctctgca tcgcggtcga actgacgccg agattcttca atgtttccga aatccccgat
    13261 tcggtttcct tttcggcgat cgcactgctg ccattcttca ttttgatcgc gagatcgttg
    13321 ccgttcacca tgatctgcgt gacctggccg gaatttatat cggtcgtcag ctggttgaga
    13381 ctcagggtct gcggctgctt cgccggcgtc atgaaaagag aaaaaagaag cgcgacaaaa
    13441 ataagcgcga ggaccgccca gaggatgttc ttaaaaagat tggacataaa agtgaaagta
    13501 aagatttagt gcgacaggtt tcagtatatc aaaagtggaa aattccgcca tatccgcgcg
    13561 tcaacgtcct tacaatgaac tcgactccgg acgtccccta atggtgttgc gatttttcct
    13621 taattcatta tgatagaaag gcgtaaagca tcatggtgcc tctagcttag tggtaaagct
    13681 ccgctctgtg gcagcggcga tacgggtccg attcccgtga ggcacccctg acaggtctta
    13741 atataacttt ctccttactc gcgcgaacct tgcgtatcgg tgccaccgtt tccgctgaaa
    13801 tcgcttcctg cgcccccgaa tcagtcacgg cgccgaccgc ttccaatgcg agtatcacgg
    13861 gtgctttcct tgcttctgcg attcgctttt gggtcaccct gaactcgttc cagaaaaggc
    13921 cgaggtagag ccgcttgagt tcgtagggtg ccttctcgta cgccttgccg atatcccgaa
    13981 tgagcatgag aatttcctga atcacgtcgg tcttgaggtt tcgcgaccgt tccactttat
    14041 gtatttcgtc gtcgatattg tcgatctggc cgcgaacctg gttcttgatc ctcgtgaacg
    14101 cgtcgtccgc caagacgccg ctcaggagct tctcctccgc gaccataagc ttcttttcga
    14161 ggttcaactt gaacgccatg agtcgttcct tttcctccga gactccggct tttttcttat
    14221 catagagttc ctttgctttt gccatgacct tctcgataaa agctggtgag aactgcagcc
    14281 ggtcaaaccg ctcctgcacc tgctgctcca agtcccatgt atccacgtat ttgtcagtgc
    14341 acttgattcg atcgccggaa cggttgcagt ggtaatacga cttccgtttc ttgaaattat
    14401 gctcggcggt gtaccgctga ccgtccaggg cgcaaaatac gaaaccgttc aggaggaacg
    14461 cgtgcttgcg cttgcggcat gcgtaccgat tattctccgc aatgacctgc tggcacctat
    14521 cgaaaatttc tttcgtgatg atgggcacat gatttccttt cttgcttttc ccaagccagc
    14581 gcatctctcc atagtagaaa gggcatcgca gaagcgcaaa catcttgctc cgcgcaatca
    14641 tcttccctgc cagtgtgacg agtccttttt tgtacgtcat atcccgcacg tctaccactg
    14701 aatagtcgcc ggtgctgtat agcttgaata tttcggcgac gagcggtgct cgcaaaggat
    14761 cagtgacgac gatccgcttt ttgggattat cagagtctcc cgcgttcatg tagcctatcg
    14821 gcgccttggt cggccaccat ccctcgtcaa atttctgatc taagctctta agcgtcttac
    14881 gggatgtgat ctgcgactgc agctggttca cgcccgcgat aaccaagtcc ataaagtttc
    14941 cttctggcgt gtcctccata cccggctggg acaccgagat aagcttcaca ccggccttcc
    15001 gaaggagcgc ttttattgtg agatgatcat tcgcgttccg tgctagccga tccgtatctt
    15061 gcacgaagac ggcgcgaata cttttatcct cgcggatacg caagagaagg tcctggagac
    15121 cgggacgatt catggtagtc gcgctcctgc caggatcccg gtaaacgcag ccctccgcga
    15181 gccggtagat tccgccctcc tcgattgccc gctcgcaaag ccggagctgg gttttgagcg
    15241 aatgccgctc gtcgccggct tgctcgtcgg ttgaaactcg acaatatatc aatgctttat
    15301 ccatgtgtgt tttgtttaaa aagaagaaat tataccatcg cgcagccgca ttattctgcc
    15361 atcgccaata tcaaaagtct gtgtgctcag ttcttcgaaa gacgcttgtt cggccgccat
    15421 cgacgcgtcc cgctgcagcc gctgattccg tttgtacaaa tacaaaacaa tgaatgccgc
    15481 tcccaccgcg attaatgata cggtcataaa cacgaaggga ccttttcctt ctccccgtgg
    15541 aagttccgca tcctcccgct ccgcgcgatt tttattattc accgcttccc gcagcctgtc
    15601 gcgatgccgc cagtaatatg tccgggcccg ttcacggtgt tgcaatctct gcttctcctt
    15661 cttcgcgcgt tcctcagaaa actcttctcc ggaatccgct gacagagaaa tgttctcttc
    15721 aagtacgttt gtcatataaa tgtgttggat ttgttttacc gacctttgat ggacacgatg
    15781 ccgccgttcg gctgccaagt caactgttcc actggcactc caaagcccca tgttttttat
    15841 tttttagctc gcagacatta ctcattctgt tatagtttct ttgagtgctc tcccgaattc
    15901 cgagccaaga tggcgccaaa ggaaatcaat catccgcgga tctccttcta tcgccttctt
    15961 cacgatcaac ttggccagca actcctccca gctcatgcgc tgacccggcg gcactttctg
    16021 caactccgct tttatccttg cggtaagcga gatgctaccg cgcggcttac cctttggatt
    16081 ccccgagcac ccttgcctga atctgcccct ttcatccctg atattacctg taaaagcagg
    16141 tagctgatgc gccgacatat cttttgtttt catcttgatc atagctatta atcccgtcca
    16201 tgcccgaatt tcttgcaatg gctcaacgtg gttgctctga tgcccgccca cggttgagta
    16261 atacggcctt tgttccagaa aatttctcga accttgcgat gataacctct gtgtagaatg
    16321 gatcgatctc tatcgcccga cacttccgtt tcataatctc gcacgcgatg agagtgctgc
    16381 ccgacccgcc gaacggctcc atgacgatgc cgccccgcgg ggaaagaatt ttcgcatacg
    16441 gcacgagaat ctgcactggc ttcgtgccaa agatcaggcc ttgcccagag gattttcccg
    16501 tttcggcgga ccaggtgaca tggtcactga ctcttgccca ccgcgtatgc tttcttttgt
    16561 cccatgcgct ttggcctttc tggccgtaca gacagacctc atacgtatct aaaagttttt
    16621 gccctttcgc cagaaggtaa tcgtccaact ccttttcgta ttcctcattt gaaactccgt
    16681 ctccggccag cggcgcgatg tcgtactttg agaacagtct gtgcggcgcc gaaaatccct
    16741 ggctccggtt cggcagccac cagatcacca tgttgcggat tttccagtac ttctccaacg
    16801 catgccacaa ttccggcgtg tttttccagt tctcgaacac catcacatta gctcctttcg
    16861 gattctggaa ctcgttcgca attgaaagcc agcggtcgta aggcgggacg ccgccccgtt
    16921 ctttcaaacc gcggtattcc cgaagtcctt tatatccgaa tcctcctttg gttttcgtct
    16981 tgaagccata cttatgcgag ccgactttcg tatcgcccat attcgcgcct attttataag
    17041 gagggtccga aaagaggaaa tcaaaccgtt cgtccccaag cagccgccgc cagttttcct
    17101 tcttcgcgca gtctccgatg agaagccgat gctcgccgag ctgccaaagg tctcccaccc
    17161 tcgcgtgctt cggcttgccg accgccttcc gatattcttt ttcaaaatcg aacgtctcag
    17221 tggttgcgac tccgaaagtt tcgtccagct cgtccgggct ccagccgaca tctttgagca
    17281 aatcttcgct gaaattcgcc agtaagtccg tatcccagtg gccttgattt ttattcaaac
    17341 ggagattcag ctcgttcacc tcctgcttgg taagttttct gtcgggcacc cggacatcca
    17401 cttcctgcac gcctttttcc cgcaagagcc ggaggcggaa attcccgccg ataacctcgt
    17461 tattcgtatt gatgacgagc ggctcggcca gatcgaattt cgccaaactt ttattgagat
    17521 ccgcttcctc tttttcgctg gcctggcggg gattacctct gaaaggaatg aggtcgctta
    17581 cctttcttcg cgaaggacgc cagattatct ttttttcggt tggttgcttc atgaatgttt
    17641 tatttcgcgg tcttctggct taggcctgca attctcaaac tcgacctgcg caatcaaaat
    17701 ttccgccagc cgttccgcag ctgcttcaat cgctttgctt tccgcttctt tatttttttt
    17761 gttctcctgt tccatgatgt ttttcgcatc ctcatcatta cacaggaatt tatcatgcag
    17821 cacatgggac gcttggtgta tgacgatatt ctcaaggcac gacgccgaag ccagggcagt
    17881 ttccgttcaa gagcggcctg cgacgtgcgt aatgtctacc ccaactaccg cagctaagat
    17941 tttcattgcg gttcctttgg tctttcagat agaaaaacag ccgcccaacg ggcggctgtt
    18001 cctgctacga atttccctaa tttaccgatg tcgaaagttt ggaaccaagt aatttttcaa
    18061 ttcttttctt ctccatatca acgatgcgag agggtatcgg attaaatcct aattttttga
    18121 cgattttatt ggctcctttg gtaggtaatt cttcctcgtt ccttaagtaa aagaaaataa
    18181 ggtttcctaa cagtttcttg taatcatgcc ttgcaagtcg tccgaattta aatctatagg
    18241 cggggaaccg tttttgggcc gtttgtaccc cgcgaagctt tttcttcact tcggaaatag
    18301 gcgtgtcagg atagattttc aagacgacgc agtgatcaaa gttctccatg atatcgcgat
    18361 aggcccggga atgatccagc ggcctccggg ttttcaagag ttcaaagaaa tcactatcca
    18421 tcattcgaaa cagatgaccc ggagaagtaa tcggatgcaa ctccggtggc cgcgtcttct
    18481 gcggaagatt tctggaagaa atgtggccga ctgaattgta aagaatgtaa ttttctaaat
    18541 cccatttcaa aataggaaac aaaaaaggca acggccccaa gcagctttcg gttaaacgtt
    18601 ccttcaaggc gggaaatttc ttcttcaccc actcggaaat ttcagacctc atacgcatct
    18661 cctcaacaat gaacttcctg ttttctggag cgtaatctat gattatcggc atcaacgctt
    18721 gcgccgcctt gccggaaaat ttcttatgta actcctccct tactttcacg ggatcaatgc
    18781 cctctgtatt cctgatgctc tcgaccactt cattgaagac gggaatgaat ctaaatatcg
    18841 tcataaatga atacagtgat gggtctcctt ttccgatctc gtattcgggc gccaaaatat
    18901 ccataacttt ttgataggct tcctcttctt tcggtgtaag cacctccgct ttttccgaca
    18961 gtttccgttt ttcaatcaag gcggtttcct ttttgcgctc aattaatgtc cgatgtaatt
    19021 ccttcaggtc atgaggagtt tggtaatctg ttttaacaaa attaaactcc ttgagcaggt
    19081 caggggttat tttcacccgt aagcctttct tattttcggc aaacataggg ctccgatttg
    19141 cctccttgca gaagtcagtt atgttcatgt cgcatctttg ttaatctttt tactaaaagt
    19201 aacgcaattt tcagcttgga tatccgaatg accggagaac ggcaagccag cttaaggcca
    19261 gaaaatgatc taccgaaagt tcgggatatg ccgcgaggag attccctcct ttctcttcgc
    19321 tctcgaaaaa taatttatag ttgnnnnnnn nnnttatagt tgtctctcat tttcagaata
    19381 gaggaatttt tagatgaaaa caaaatactg atacgctttc ggtataacac ttgccccaca
    19441 ccacccgtgc gcgcgttata aagcttttta ttttttgctg atctaagaag atcggcaaaa
    19501 ctcccttgta ttgaaattta taaaaggtta tgcagggcac gccatatcgc gaacatcgcg
    19561 taggtatcaa ggccgcaata ggtttttaag ttggcgatgg cttcttcttt ttctttctcg
    19621 ttgaaagagc cgttcgtgat cttgttccac gtttcggccg cagctcctcc ttcgtggatc
    19681 gcaagttcct tataagaaag ttcggggacg agaacgggta ggattttctt gatcgaagaa
    19741 ctgcccctga aatccttgtg gacgtgatat tgttttttga aaatatcttc caagtcatag
    19801 acgcgggcgt tcacggaatc cataaacgcc ttggccgaag gaactctcgc ggcgatctcg
    19861 atattccttc cgcactcgaa gcttttatgc caaacgatga cgctccccgt ttcgcctatg
    19921 tgcttttgca aagattccgc gaacggttcg ctcggatcgt cggctccggc gtagagaaaa
    19981 tcataatttt taggttcgcc tttttgagat tccaaaacct gaacggaata ctggaacggt
    20041 atctgattga aaggagaata cccgtcgaag cgcgggatcg ccgacgggaa cgtttcgtaa
    20101 tccaagaaat acagagggaa aaccaaagtt ttcagatcat ccgcgatctt ttccttgtct
    20161 accaaaaccc tatcggaaac ataggcatca acttgcccct gttgaatctc gcttagcttg
    20221 atatgttccg gaatcttatc gagatggaaa atattgccgt ccaccagctc tttcagcttc
    20281 gccttactgt ttccaatgcg ggcgatgtcg tggatgccgt attccggaac gtcgagattg
    20341 gaatgtttaa aggttgagca atggcttgat ctgcctttat agatacagca gcaagaaccc
    20401 ttcggttccg cttcttgcga gagatatttc aatgccgtcg ccgcttccac ttctatgcca
    20461 tttataagat tatccacttc atggctcaca tcgacgatct taaaaagttc ttccaagttc
    20521 agctcgccat gccggacata ctcggcattg agatggataa gatacgcctt atctattttc
    20581 acgccgcatt tcttaagcag attcacctga aacgtgagat cgtgataatg caccttatca
    20641 tcaatgctgt tcgtcgattt tacttcatag atcgaatatc cctgcgttgc gggatcgtat
    20701 ttcaaaatat cgacggcggc caagtagtta tctttgacga aaagcggctg aaacagcact
    20761 tcctgtttct tcacgagata gtcctgcgtc gccgcttgcg ccttctcgtc cctaccctcg
    20821 atcaagatgc cattggggaa taattttcga gccaccagct cgacatcatt tccggtttcg
    20881 ataatggact tctcgaactc tgacaactcc gattcaaaat atatgtccgg cttgtggatt
    20941 ttataccaaa catttttagg acattcgcga tatagaatat aatcggtttt tgagagcgtg
    21001 atcggcatgc gcggattatt tattcttgcc gatttgaaga tcggctactc ccttttatag
    21061 ttttataacc tataaagaaa gtcctagttt atctctcctt ttagctaatg cttttttacc
    21121 tccttctaac acttcaataa tttcgtcctt aattattggg ttctcaatag caccagaaag
    21181 atatttaaaa ttcgggtttc tgcctccatg tttattcgat acaatgactt catcgctatc
    21241 gccgcataca acaaggtttg cattgtgagc cgcaacaata atttgtcttt ttattttctc
    21301 tttttttata aaatcaacta tgtgctttga gatc
//
LOCUS       DAIDDT010000006        19412 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_6_length_19412_cov_5.809723, whole genome shotgun sequence.
ACCESSION   DAIDDT010000006 DAIDDT010000000
VERSION     DAIDDT010000006.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19412)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19412)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19412
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6_length_19412_cov_5.809723"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4855 a         4520 c         5236 g         4801 t
ORIGIN      
        1 ccttgaaata tcctgatccc gatattcttt cgttgcaatg cgcgctttcc gctccgaaaa
       61 ttcctcgcaa tccccgcggg tctattttcg aggcaggttc tataagtgga tgtggttttc
      121 cgtcgtcttt gcgaaggcga tagaagcgat tttgtccgcc ttttttattt ttttctttgt
      181 tctataatga aaagatgaag attcgctgga agaaagtgac atggtattcg agatattcgg
      241 cactcgcact ttttgtcata ttgccgttcg caggttttta ttgcggcgca caatacggat
      301 tattacaggg atatatcaat ggcgttgacc aggttttatg gggaggaaaa gtattgacgc
      361 tgaccgcggg gaatggttat tacggaaatg tcgcggcatg ggttgtcgac cgcggcaacg
      421 gtttcactat ggcatatccg cttgatttcg atgcgcaaaa taatgacacc ggtgcgctgt
      481 ccgcggactg gcggcttggt gccaatggcg cggcgggaat aaagatgctt accgtcaccg
      541 ttccgcgcgc attcgagccg cagacgaatt tcggcggcgc tacggttatt gtcgggcgga
      601 gcggcagcaa aagcgcggtg gcgcagtgtc ttatcgccga tccgaatgcc ggcattgcgt
      661 cgacttcgac ggaaagcata aacggaatca gatttttcgt ttcgaattcg accggcgtcg
      721 gtgcgggtaa ttattacgag acaacgagct atcgcacggt ccgcgcggga caatgctatg
      781 ccgtcgaata taccatccat tcgacgcaaa tcatgaatta tccggcattg tacggccttc
      841 gcgcgttcga taaaatgaaa atccagtcag tgcttcaaaa aatcgtcggc acgttccgtt
      901 ttgaataaaa cggcaagcgg aacgccggtt ttttaaaaat ataaattcat ggcaaagaaa
      961 aaaataacaa cgaagtggtg ggcggttgcc atcgttatct tcatggtttt ttcaatgatc
     1021 cttttcgcag ccggaggaat tctctcggcg ttgtccggcc gatagagggg ggctgcgcgc
     1081 ctccggttgc cgccgttatt ttccatggga cgctttcacg atcggatcca tagtgctttc
     1141 cattattttt tttcgatggt gtgccatttt ttcccgatgc ggtggtcgtc ccgctcaaga
     1201 ggcgggttat aaggattttt gcgtgaccga aaaagctttc ataattggat caagacctcc
     1261 ttccattatt ttttctatgt tatgaaactt tttccccaac ctatgatcgg tcacacggtc
     1321 gtcggggaaa ttataggtgc ggattttttc ggaacggtcg gcagtgccga tctgttcacg
     1381 gcggagatcg cccacgttac cggtggcttt ctgctgttct atttcataaa gtttcgcacg
     1441 gagcacgtcc atcgcttttt cgcggttggc atattgcgag cgttcttcgc gggatcccac
     1501 gacaatgccg gtcggtttgt gcaggatgcg cactgccgtt tccactttat tgacgttctg
     1561 tccgcccggt cctcccgcgc gcgagaacgt cacttccaga tccccctgat tgatattcac
     1621 ttcctgcgct tcgacgaccg gaagcacggc gacggaagcg gtcgaggtat gcacgcggcc
     1681 tgatttttcg gttgcgggga cacgctgcac gcggtgcacg ccggattcgt gtttcatggc
     1741 gtcgtaggtt ccctctcctt tcatttccgc ggtcaaactt ttatagccgt tgatcgccgt
     1801 ttcgttctta tcgagaacgg tgaacgtcca tccgcgcgtc gcggcgtagc gctggtacat
     1861 gcgcgccagg tcgcccgcga atatggcggc ttcgtcgccg cccgcgccgg cgcggatttc
     1921 caatatgatc ttgttcgttg tcatggttga agttaacagt aaaaagtgaa aagttttaga
     1981 acccgttcat aatctcatgc cgaagcaaaa ttcatgaatt tttgccggca cgcattgctt
     2041 ttatcggata ttatccggtg agcttatgaa caggttcttg ggtggcgcaa caacaggtct
     2101 gttgtcctcg cgaaggcgag gatcgacgct tccaatctta tcttatttcg tgaattttga
     2161 ataatgaatt tctatttttt aaagcacggg ttcccgccgg agtttaccct gtgccacgat
     2221 acagggcggg aatggcaggg agtttgtttt aagcgcgccc tttcctgaat aacatcaaaa
     2281 gaataatgag cgtgaaagtt gtaagcgcca tcgtcgggat cgtgacgtag ccgaactcga
     2341 tgaaatagcg gaaggtacag cttggtcccg cgcccgccgc atcacaggaa atgaacggac
     2401 tgccgccgta ttggaggtag gagtgatagc cgcttatcac ggctcctatg acggagaacg
     2461 taaggcagaa ggcgcggata tgtttgtctt tcacgaatgc cgccgcggca aggacgaatg
     2521 cctgcggata aaggaatatc cgttcccacc agcagaggac gcacggcgcg aagccggcga
     2581 tttcggaata gaacaaactg cccagcattg cggtcatggc gataatgccg gaaaaaagaa
     2641 ccgcgttttt ttcaaaaaat tcagccgcac ggcgcgtgaa ggaactttcc tcttttttcg
     2701 gcgtgatgag gataaaaaaa aggaatgcgg cgaaaaaatc gcagagcaac gtcccgaacg
     2761 acaagaggtc ggtgaatgtt tggacaaggg gcgtcatggg acgtagggag ttatgaatac
     2821 ttgggatatg ctcgaaatat taaggaagtc tattgcgttg aagtcggcag ggggcagccg
     2881 gttttctgtg cgagcgtggc gagcgggact tcgcccgtaa gacgcgagcc gtctttgaac
     2941 acccacgtgg gatagctggt aacggaagcg tccttgcaga tctgcaattg cgcggcgccg
     3001 tccggcgttg aacactcgac atacgggagg aatttcacgg catcgccgaa catcgctttc
     3061 tggttctggc agtgcggaca ccagaacgcg ccgtagaacg tcgcgccgct cgctttgatg
     3121 cattgcgcaa aagtatcgta ttttccgttc ggcggcggaa gatatttaag atagacgaac
     3181 gccgtcgcgg cgacagcgag agctatggcg acccacatcg ttattttaag ttttttcatt
     3241 tgccgcgcat gggcgggatg atggtgcgtt tcttttttca taataaaaaa gaatggatat
     3301 aatatttttc aatggtagca gaaaaaaaat aaacttgcaa aatagtcatg attatatgat
     3361 aaatgataaa gaatgaaaaa agaaaaacgc tttatcatcg gtatcgacga agtggggcgc
     3421 ggcgcgcttg cggggccggt ggtcgtcgga gccgttatga tgccgtgcgg taaaagaatc
     3481 ggatttccgc ggcgtttggg gaaattaaaa gattctaaaa aactttccgc gcggcagaga
     3541 gaaagatggc ttggatattt caacagcagt ccggatattt tttctgccac ggcccgcgtc
     3601 tatccgcgcg gcatcgaaaa aatgaatgtt tcgggagccg cgaatcgcgc cgcttcgaag
     3661 gctttcggga aagtgagcgc cgccgcgccc ggagcatggt ccgtattaag aatctatctt
     3721 gacggcggat tatccctggg aaacgggaaa gggggcacaa accggaaaaa tttaaaaaaa
     3781 gaaaccaaga tcgcgagaac agttataaaa ggcgatgaaa aaataacggc gataaaaatc
     3841 gcgtcgatta tcgcaaaagt ttcgcgggac cgttttatgg tgcgtctcgc taaaaaatat
     3901 ccggcgtacg ggttcgatat ccataagggc tacggcacca aagcgcactt tcgggcgatc
     3961 cggaaacacg gcgcgagcat cgtccacaga aagacatttc tttgaggaaa cgatttttgg
     4021 tcttcaaaat aaaaatactt ttatcgaaac gatcgtttcg ataaaagtat tttgaagaca
     4081 tatgcgctgt ccctccgggg attaaaagat tattgacgcg ctatattctc ctgtctcatg
     4141 gattgtccgt cgaaacaaaa acgcgccatt ggaacgcatt tttgcggcct ttttattttt
     4201 ttaaaatcac ctataataac aaccacatga gattcaagac tgaatttgag cgcgctacgg
     4261 cgctgttctg ttctcttgct gaagcatgat aacattttta tgaaaattct cttttttgct
     4321 gtttcggtgg tattgcagat cttttttctt tttttcagct tcgttattta taaaacgatt
     4381 gtttccgctt tcggtgtcgg aggatggctt cttttggtcc ttcttttggc gttttcgctt
     4441 tcattcgccg cgacggcgat tctcgcggcg cgctataaaa ataaactggt gcgatggttc
     4501 cattttttgg gcatgtattg gcttgcgttt atcgggcctc ttttcctcgc gtgcatcgcg
     4561 ttcgtgttca tgaaaaccgc cctgccgttt ttcggatgga acatccattc cgttaccgcg
     4621 gggcggatat cgtttacggg cgccgcattg ctctatatat acggcgtatg gaaaggcgcc
     4681 gaagcgacga cgacgcggat cacggtggcg atccctcatc ttccggcgtt ttggaagaac
     4741 aagaccatcg cgttcataag tgacgtgcat cttggcaacg aatatggcgc gcggtttgcg
     4801 ggggagatcg ttcggaagat agcgtcgctt tcgccccagg cagtttttat cggtggcgat
     4861 cttttcgacg gagtgaagtg caatcccgac gtccttctcg cgccattcag gaggttgcgc
     4921 gtcccccatg gcgtatattt tgtgagcggc aaccatgaac atatccgcga caacggcctt
     4981 tttttaggcg cgatccggcg cgcgggaatc atcatcctga acaataaaaa gaaaaccgtc
     5041 gaaggaattg attttctggg tgtcgatttc aaagacgccc ataaaaagaa gaattttgaa
     5101 aaagtactcg cggagcttgc gctcgatccc gcacggccga acatccttat caagcacatt
     5161 cccgataatc ttcatgttgc ggagcgggca ggggtgtcgt tccagatttc cgggcatacg
     5221 catcacggcc agatatttcc gctttcctat ctcacgcgcg agatttataa aggatatgat
     5281 tacggcctga agcaattcgg aaaaatggcg gtttatactt cgagcggcgt gagcgcgtct
     5341 ccggttcctt tccgcatcgg aacgaaatct gaaattgtcc ttattgaatt taagaattta
     5401 gaatccagaa tataaaaaat aaaatagaga atatagaaaa acactgctct cggtcatatt
     5461 ctctattctt atgtttcatg gttcagaaaa aaaataattc gcgtggtttt gcctggtttg
     5521 cggtcatcgc cgtcgcggct gctttggcgg ctatcgtatt tatcgcagta acctcatttt
     5581 ttccgcggat cggaacggcg ccgttgaata atggcgtttc cggagtggcc gtatcttcgg
     5641 gcgtcatgac ggcggccgga acgatgccgc gcaccgtttc cgccgcgatg ccgtcctcaa
     5701 cagcatcctc gacggttgca agaaaaatcg caaagccgcc gcatatcgcg tcaataaatc
     5761 cggggaatgt tccgtcgggc gttgcgacgg ttccgcttcg ggtttccggt gacatggctg
     5821 ccgcgccgac gctggttccc gtcgcgcaat cttcgaccgc cgcgatttcc gcatcatcat
     5881 cgaacaatat ttcgagtccg ctgccgcccg aaatgtccat caatcccgat gcgatcgtgg
     5941 ggatactgtg ctatttcaac gttacgttca ctaatccgca gaacggcgcg attgcaaccg
     6001 ggtcgcagga agaagtaagg ggttcgggtg tcatcatcga cccgcgcggt tatattctta
     6061 cgaaccgcca tgttatcgag cagcccgaag cgaccacgac ggctcttgat gcgaacggaa
     6121 acaaggtacc cgctgtcgtt gattattcgc tggatcattg cgaggtcgga caggttgcgg
     6181 cggattccca tctcccgacg ccatccgaga ttgaatcgct gaacccctct atccaggttc
     6241 cggtactcgg ttatacggcg caacccgttt acatatcgtc cacgtccggt ttgagtgtga
     6301 acgagatcgc cgccgctgat tttgcgattc tcacggtcac gggaattacg aaagcgggac
     6361 aggccttcgg aatgacagcg gtgccgtcgt tttttccgta tgccgaactt tttcccggcg
     6421 cgcaatatct tacgcccggc acgcaggtcg tgacgtacgg tttccccggc gatatcacga
     6481 cggagcaggg aaattcattt gagacgttga ctatgacggg aagcgtcggc acggttacgg
     6541 agatcgaggt cggcgatctt ttttatgccg atacgccgct catcattctt acggatatgg
     6601 acgttgcgca cggccgttcg ggttcgccgc ttttctggag gggctacgtc gttggattgg
     6661 tcacttttta tatcggcgac aaccaaacga gttccggttc cgtcgcaagc gaagcgattc
     6721 tcagggcctt gcgtttaacg gattacaatc cgatttacga ccgataagaa catatacgaa
     6781 gaatttcggt gttaagaata cggattgtct gatttttcct tacggtttcc gccgcggggt
     6841 tatccgtcgc taagcgtcgc ttgttgcctc gcgcggcaac ggatgggata atgtctttat
     6901 gataaaagaa aaaaatcttc cggcgttaaa aaagcgcgca ttgaaaatca tccgcgttct
     6961 gcggaaacta tttcccgatg cgaagatttc gcttgactat tcgaatgact gggagctttt
     7021 ggttgcggtt gagctttcgg cgcaatgcac cgacaaaaaa gtgaacgaag tgacgcggac
     7081 gcttttcaaa aaataccgta cgctgggcga ttatgtccgc gccgacaggc ggacgtttga
     7141 acgggatatc cgcccgacgg gattttatcg cgcaaaggcg aaaaatattc tcgcggcggc
     7201 gaaggtcgtg aaagaaaaat tccgcggcaa acttccgaag acgatggaag cgatgctcac
     7261 tatccccggc atcggccgca agtcggcgaa cgttattctg ggaaacgctt atggcattac
     7321 ggaaggcatt gcggttgata cgcacgtgaa gcgtctggcg cgcgtgctcg gcttatccgg
     7381 cgagaaaaca ccggaaaaaa tagaacgcga tttgatgcgg atcattccga aaaaggaatg
     7441 gcttcgcgtg acgtacctct taatagagta cggccggaac tattgcgtcg cgcgcccgca
     7501 taagcatgaa ttatgtccgc tgacaaagat tttgaaatga ggcggctttt ttctccccgc
     7561 gcggccgccg cggccgcgcg gggagaaaaa agaacgatga aagtccggag aacgccgcgt
     7621 tttccgccgc cgcgccgcgc gcagcggcgg ccgcatgcgg cgcatcgtca atagctgcct
     7681 gtggataact cttatttgaa gtttacggcg aagtgctata atggtttttg aaagaaaaac
     7741 cgctttccgg tgtgaggccg gaaagcggtt ttctgatata atagaaccta tgaaatcggc
     7801 gcatttcttt gttgcgctgc ataaaatatc gctcacaata ccggagtatt gctcgcgata
     7861 tcttgttggc gcgctttgaa ctgcatccga ttttacaggt tccatgaaaa acacaaaatg
     7921 accgataaga aattcgaaat agtttcaaat aataaaccgg ccggcgatca gccgaaagcg
     7981 atcgcatctc tggttgcggg tctcaaaaaa gggtttcgcg accagacgct tttgggcgtt
     8041 acgggatcgg gcaaaacatt caccatcgcg aacgtcatcg cggcaatcca gaaaccgacg
     8101 ctggtcatcg cgcacaacaa gacccttgcg gcgcagctga cgaacgagtt ccgcgaaata
     8161 tttccgcata attccgtaaa ctatttcgtt tcctattacg attattacca gccggaggcg
     8221 tatatcgcgt caagcgatac ttatatagac aaagaagcgc tcatcaacga cgagatcgat
     8281 aagctccgcc atgcggcgac gatggcgctt ctcacgcgcc gcgacgtcat cgtggttgcg
     8341 tcggtgtcgt gtatttacgg tttgggcgcg cccgcggcat atgcggaaaa tattttccac
     8401 tttaaagtgg gcgacaaaat cgaccggaaa acgtttgcga gaaaactggt tgaactgcaa
     8461 ttcacccgca cgaacgccga cctgaaacgg agcacgtacc gcctgcgcgg cgatcattgg
     8521 gagattatgc cgccggaccg cgaggcgatc tatgactttg agttgggcga tggcatcatc
     8581 aaagccattt acgaagtgga tcccgtcgcg ggattccggc ccggaaaaaa tccgacgctt
     8641 gcggaggttt atatcgcgcc ggcgaaacat tttgtgaccg gcgcgcccga ccgcgaccgc
     8701 gccatcaaag ccatcaagca ggaactgaaa gaccagctcg cgcttttcga taaagaaaaa
     8761 aaatttctgg aagcggaacg gctcgatcgc cggacgaagt tcgacatcgc gatgatgcgc
     8821 gaagtgggat actgccacgg catcgagaat tattcccgtc atctttcccg tcgctctgcg
     8881 ggcgaaccgc cggaaacgct cttggattat ttccttgcgg gcaacagctt gtcgggcggt
     8941 gcgcataaaa aaggcggcgc attcccgcag gccgattttc ttaccgtcat cgacgaatcg
     9001 catatgaccg tgccgcaatt gcaggggatg ttcaacggcg atgcggcgcg caaaaaaacg
     9061 ctcatcgagt acggtttccg tctgccttcg gcggcggaca accggccgtt gcggcttccg
     9121 gaatttgagg cacgcatcgg tcagaccatt tatacatcgg cgacgcccgg gcaatatgaa
     9181 ataagaaaga gcaaagaatc ggttgaaaaa atccggcatc gcggcgttaa aatttccgtc
     9241 gaagaaacgg ggatcgtcca gcagcttatc cggccgaccg gtcttgtcga tccaaagata
     9301 accatccgtc cggcgcgcgg gcagattgat gaccttatgc cgcgcataga agaacgcgtc
     9361 gcaaaaaaag agcgggtgct tgtgacgaca ttgacgaaac agatggcgga agacctaagc
     9421 tcatatctcg aagaaaaagg agtcaaagtg aattatctcc atagcgacgt gaagacactc
     9481 gaccgcatca ggattcttac cgacctgcgc aagggcaaga tcgaggtttt ggtcggcgtg
     9541 aatcttttgc gcgaaggctt ggacttgccg gaagtgtcgc ttgttgcgat cctggatgcc
     9601 gacaaggaag gatttttgcg gagcgagacg tcgctcattc agacgatcgg ccgcgcggcg
     9661 cgcaatgtgg aaggagaggt gcttatgtat gccgaccata tcacgggctc catcgaccgt
     9721 gccgtgaaag agaccgaacg tcgccggaag atccagcttg cgtacaacaa aaaacacggc
     9781 atcacgccga agacgatcgt caagaaaatc tccgacattt tgccgatcgc cgacgccgtg
     9841 cttgcgctgg aaatgaaacc gataccgaaa tcgaagaccg cgctcaaaga attgatcgcc
     9901 gagaaggaac gtgaaatgcg cgcggcggca aaacacctcg attttgaatt ggccggaatt
     9961 ttgcgggacg aaataagagt actgacgaag aaggcggaag aataggtttt aaatatcagg
    10021 ggcgggaaaa gggtgcctgg cgccattatc ttttctttcc tcgaagaaag aaagatgtag
    10081 aaaaataaat aaggaaatga ttgaaaaatc agaagaagaa gttaagcaaa aattagaggc
    10141 gctatttaaa cattactggt tacttttaaa aagtggacga gtacctttat tttccttgac
    10201 aatgcgtttc gcttcgctat aatggaacga ccccttaagg aaggggccgt tctttgaaaa
    10261 ccgaatagca ttaaataaaa accctccaaa attttgccaa acaaaatcga agagggcaaa
    10321 gagagttttt tagttgtccc aatatatttc ttttcacggg attttcttgg aacctatttc
    10381 aaaaatagtt tctggaagaa aatgccgcac aagagtgaaa ggctcttttt aagaggattt
    10441 gatcctggtc caggatgaac gctggcggtg tggataaggc atgcaagtcg agcgggatcc
    10501 attttgaaga agttgcgatt acatcaagac gacggattta tggattcagc ggcgaacgag
    10561 ttagtaacac cttggtacgt acctcgaagt cgggtatagc tttccgaaag gaagggtaat
    10621 ccccgatatt gtcgaaagac gaaagtcgca agacgcttcg agatcggcct aggcctgatc
    10681 agctagttgg tagggtaacg gcctaccaag gctatgacca gtaggggagg tgagagcctg
    10741 gcccccaacg acggaactga gatagagttt gtctccttcg acggcaacgt tgaagtaaaa
    10801 ccctgctcat aacggtgaaa cccattgcgg tggtttggaa atgtcaaata tctttttatc
    10861 attatttaac ctccgaggtt ttataatgtt tgacatgaag caaaaccgcc gatctcataa
    10921 acaatggtca accccgtggg aagttcatag tttcctgttt tcggaagcgt atgtggctgg
    10981 tttcttggat ggtgacggtt cggtggtcgc aacggtaacg cggcagtccg agcgatatcg
    11041 ctttccttgg agaatgtatc tgagaataaa ttttacgcaa cacattcggc ataaagcaat
    11101 gctggagaac ttgcaggaat ttttaaacgg atacgggcat atacgtatga taaaaagtca
    11161 tcatttggcc gagcttgtca tacaagatcg cggtcaggta aaagcagttc ttgaacgttt
    11221 gattccccat cttattttga agaaaggaca ggcacggcgc gcgctcgcga tcatcgcaat
    11281 ctacgaggcg agttttaaaa gcaagaatgg caaaagcttt ttatcagaaa aacaaagaaa
    11341 cgaggttttt gccctgattc aagaaatcag aaatctgaat tcgagaaccg gcggcaagag
    11401 actcaaggaa ttatgtaacc ccgtaacgac ttctccagaa aatggaggga gtcaggaaat
    11461 gtgatgttat tatttcttgg ctaagacgca ggcccccgaa accgaattcg gggtgaaggt
    11521 atagtctaca ccatacgtaa gtatggaatg atgtgacggt ccgtactcct acgggaggca
    11581 gcagtcgaga atattcgtca atgggcgaaa gcctgaacga gcgacaccgc gtgcaggatg
    11641 aaggccttcg ggtcgtaaac tgcggtaaat aagtaacaat gcaaatgagt gcttattgga
    11701 aagaggtggg taactacgtg ccagcaccag cggtaatacg tagacctcaa gcgttatccg
    11761 gatttattgg gcgtaaagcg cgtgtagggg gttttgcgcg tcttctgtta aagcccacgg
    11821 cctaaccgtg gaagtgcagg agatacggca tgactagagg atgttagagg tgcatggaac
    11881 acacggtgta ggggtgaaat ccgttgatat cgtgtggaac accaaaggcg aaggcaatgc
    11941 actgggacac tcctgaccct gagacgcgaa agcgtgggga gcaaaaagga ttagatgaaa
    12001 gctgatgccg gtcgcatgat cggcggcagc gtgtctcttg tatcggtaac gatacatgac
    12061 aacttggcta tatgctggaa catctgcgaa tgtcgaagta cccgaaatta tcgaagggtg
    12121 acaatcttcg aacgatgcag acaatcagca ggaaactcgt attttggaaa atgggatatt
    12181 tgcatgtcaa aatcattccg gatagccgga ctttccattg gaatggacca cgcgctgatg
    12241 gctttccagt tatggtctca ttgctgatat aaaatggaat aggtttgttg gcataatcgc
    12301 aaaaaatttc ccacagggaa gtccggggct gacaaagccg ccgaacttcc cgcccatttt
    12361 ccaaaatatg aggatcctca gagactacat gccgagctcc tttagtagtt tgccgattcg
    12421 ctctgggcga atccgcgaac cgaaggatga agatatagtc cgatctccgt ggcgacacgg
    12481 agagacaggc agaaatgccc tgtcccgccg ttatctctat aaaattaata tagagcgtgc
    12541 ggcgcgtaac aataatgacc cttgtagtcc acgccctaaa cgatgcctgc ttgctatttc
    12601 gagtatcgac cctcggagtg gcggagctaa cgcgttaagc aggccgcctg ggaagtacga
    12661 gcgcaagctt aaaactcaaa ggaatagacg gggactcgca caagcggtgg agcatgaggt
    12721 ttaattcgat agcaaacgag gaaccttacc agggcttgaa atacttactg acagccggtg
    12781 gaaacatcgg ccttctcaca aggacagtaa gcacaggtgc tgcatggttg tcgtcagctc
    12841 gtgtcttgag atgttccctt aagtggggta acgagcgcaa cccctattgc gtgttatatg
    12901 tgtcacgcga aactgccccg cccaaagagc ggaatgcgga attttgaatc gagcagaaaa
    12961 caaaaactgc gcctttctta tttttaaatc gtattctgtt tttcgggcgg ggaggaagga
    13021 ggggatgaag tcaaatcagc atggcccttt gatgccctgg gccacactca tgctacaatg
    13081 gtcgggacaa tgggttgcca acgcgcaagc gggagctaat cccatcaaac ccggccccag
    13141 ttcggattgg gggctgcaat tcgccctcat gaagccggaa tcgctagtaa tcgcatatca
    13201 gctatggtgc ggtgaatacg ttctcgagtc ctgtactcac tgcccgtcaa gccaagggag
    13261 ccgggaatag gcaaagtccg cctcggcgga ccagtctagg ctcggtgaca tgggctaagt
    13321 cgtaacaagg cacgcgtagc ggaagctgtg cgtggatcaa ttaaactatt tttcaataac
    13381 tcaaacgttg aagaataatg tcgaacgcgg ataatgcagg cgatacgcag acgtacgcgg
    13441 aagcaattcg gcgtctgtct gcgtttcgcc ggcgaaagtc ggcgtgatat atcggggcag
    13501 ctaaaaagct cttttgtaat gcgcagcaca ccctattgaa ataggactta cgcacaagcc
    13561 gttgcggggt ttgcaaccga aattcatgaa tttcggccgg cacgcattga ttttatttga
    13621 tattatgcgg tgagattatg aataggttct cagtcctatt gaaagatgac gggttgcgta
    13681 ttataatggc atcatgcccg aagaattaaa gagaatcatg gttatcgagg acgacaaatt
    13741 tttgtcttct ttgatgaagg tgcgtctgga gaaagaaggg ttcatcgtta tccaggcgtt
    13801 tgacggcgaa gaagccgtcg agcttttaaa aaccgatcgg ccggatcttg ttgtcttgga
    13861 tcttatcatg ccgaaggtga cggggtttga agtgcttcag gcgatttcca tgactcccgg
    13921 actcgaaaga acgccggtca tcatcttgag caatcttgca caggattcag acatccaaaa
    13981 agcgaaggag ttcggtgcgc ggggatattt cgtaaaagtg aaaatatcgg ttgaggatat
    14041 cattaaaaaa ataaaaacat tggcgggatg ataaataaaa agaaagcatc gttcgatatt
    14101 ttatagcgcg atagagcaac gtaatcacca ttttttaaaa tataatacta tgattaccga
    14161 ggaaatctta aaaaaaataa agccgggagc gaaggtgaag gtctacgaag gaaagacgcc
    14221 gttccagggc gtcgtcatca gccggaagca cgggcttgag gcgggcggaa cgttttcggt
    14281 ccgcgcgatc gttgcgggcg tcggggtcga aaaagtgtat ccggtgcatt cgccctccat
    14341 cataaaagcg gagatcgttt cgagtccgaa gaaggtcaag cgttcgaagc tttatttcgt
    14401 aagggatgtt tcaaacgcgc ggataaggca gaagcttgga atgtcgcttt aaaagcaatc
    14461 gtccattttt atgaaacggc atgctggttt atggttggcg ctgattatca tggctgcggt
    14521 cgcggccggt gtttttgttt atccgaaaaa tttcggcgcg caggagcgtc cgtggcagct
    14581 cggctgtgat cttgtcggcg gcgcgaatct ggtttatcag atagatcttt cgaaagttcc
    14641 ggcagccgac cagggttccg tcgttgacgg acttcagggg gtcatcaaaa aaagagttga
    14701 cctttatggc gcgagcggat cgcaggttta taccggaaaa tcgggcgacg atactcaatt
    14761 atacgtgaac ctctgcggta taaaaaattt gagcgccgca atacagcaga tcggcacgac
    14821 gccgtttttg cagtttgcgg aagttgatca atcgccgtct tcgacccagc cctatattcc
    14881 gaccaatctc acgggccagt atatcacggg cgcgcagctt acgttcgatc cgacgaccgg
    14941 cgcgccgcag gttgcggttg atttcaacag cgaaggcgca cagatcttcc agaaaatgac
    15001 cgctgcgaat atcgggaagc cgctggctat ttttttggac ggccagctta tcgaagcgcc
    15061 gacggtgaag gaggaaatcc ctgacggaaa agcggtcatc agcggcggca atatgacggt
    15121 tgatgcggcc cgccagcttg ttgagcgctt caacgccggc gcccttcccg cgcccatcac
    15181 gctcgttgac cagcggacga taagcgcgac gctcggcggt aatactttga aacaaataat
    15241 catcgccggc atcgtaggaa ccatcctggt gcttcttttc atgctgttgt attacgggat
    15301 attgggtatt ttttcatcgg ccgcgctcat tatttacatc gcgcttacgc tcggcatttt
    15361 caagatcatc ccgatcacga tggacctggc cggcatcgcg gggttcgtgc tcaccatcgg
    15421 catggcggtg gatgcgaaca tcctcatttt cgagcgcatc aaagaagagc gtaaaaaggg
    15481 gctgccgtat tcttcggcga tcgaggaggg tttccgccgc gcatggcctt ccatcaggga
    15541 ttccaatact tcgacgatta tcaccgccat tattctttat tatttcactt cggatttcgt
    15601 ccgcggcttt gccctgacgc tcctcatcgg cgtgctggtg agtttattca gcgctatcac
    15661 tacgacgcgg cttttcctgt tcgttttttt gaaagagaaa aaaccggcgg taaaatcgcc
    15721 cgctgtcaat aattaaaatc actgataatc atgcttaaca tcgtcggaca taaaaaaatc
    15781 tggtttgcga tttcggggac gcttatcatc cttgccgttt gcgcgatgat atttttcggc
    15841 ttcaaacaga gtattgattt tcagagcggg atgcagtggg aatttaccgc catagccgac
    15901 catccgtctc ttgcggatat gcagaatatt tttgcgaata atctcggcat ttccaacgcg
    15961 caaatcagtt tggacgcggc aagccagaca ttcatcgttc gtttcggttt gatagacgag
    16021 gcgacgcatc aaaaattgga aaccgcgata aaaaaccagt tgccgtcgtt tcatgaactt
    16081 ggattttata ctaccagccc ctcgacagtc gcatcgctcc ggcagaaatc gatcatcgcg
    16141 tttcttctcg tgatcatcgg catttccctt tacatcgcct atgctttcag aaaggcgtcg
    16201 cggccggtga gttcgtggaa atacggtttc gtcgcgcttt ttacgctgtt ccatgacgtc
    16261 gtgattcctg cgggcgtgct cgcgctgttg gggcatttac agggcgtcga gattgacagc
    16321 aatgccgtcg tcgcgctgct ggttgtgatg ggtttttccg tccatgacac gatcgttgtc
    16381 tttgaccgca ttcgtgaaaa tcttcttctc gaccgcggca ggacggcgtt caaaacaatc
    16441 gtgaacaaca gcgtgaacca gacgatggcg cgttccatca acacatcgct cacgctcatc
    16501 cttgtattgc tcgcgcttta tttcgccggt cccgcaagcc tgcacgcgtt cgttattatc
    16561 ctgctcgtcg gcgtcacgac cggtatctat tcgtccatat tcatcgcaag tccgatcttg
    16621 tcgcttgccg ctccgaaaga agcgaagcgg tgacgatcgc gttgcggcga tgattttata
    16681 ttagaagaat tttccgtccc ccccctcgcc ccgtggagta ggcgaagccg ccactctaca
    16741 gggcgagggg gttttcaatc aaattgcgaa tcttgttccg tcagcgccgc ggggaagaac
    16801 ccctcgcgtc tctctttttg gtcgttttcg cgaagtcaag aatctatccc tgtccgtcgt
    16861 cccctcgaag gaggggatcc atgcttccaa tcttaaattc ttaaagcatg gatcctcagc
    16921 caagctgagg acgacagagg gggaatgctt tgacggaatt ggcgctgatt acgggcggcg
    16981 agggttattt tttgttgaca taatacgagt atctgttcta tcattttcgc agtgctattg
    17041 aaaattaaat aaaatataaa agggaggttt tatgaagaaa gtagacgagt tctacatttt
    17101 tgacgccatc catgaacgtt ttgtgccggc gtggtacgag ctttcttttc ggaagaagct
    17161 gacggaaggg gatattagat gtgatgcgcc ggtttttgta ttgcgtatct atcgaaaaat
    17221 aatagatacg cttttcaaaa agacggccga gttggcgcgg ttagatgttt ggatgccgct
    17281 ccggtcgaca tttctttttg aaagagtgcc gcggcggaaa gatgaaggaa tgttccacga
    17341 aatagttatt cctttgccgc aggtagtgtt tgaaacgaat aatagctgcc tgacgtgcgg
    17401 cggcggcggg aaggataccg atattggcgc aatgtgcagg atgtgcgacg ggacgggtca
    17461 cgtccttgag tatcgctggg acgatgcgcg catcttgtgt caccgccttg agattctcct
    17521 ttcagcgtta cggcatattg attgctgtgt atctggtacc gcgccgcaat tgttcaccgt
    17581 gaaaatcttt ttcggtcaag gtaggccgct tggcagctgg atgcttggcg gcgatgtcac
    17641 gccggcgttt gccggatggc tttcatcgct tcctgtgcgt gagaatataa aagaagcgac
    17701 tacggcaatg atagtcgcgt ataattatat cgttggcgcc cacatgaaag aggacgactc
    17761 tggttttcgt acatgggcgg aaaaaaacgg gggattttat attcaatgtc ccggcaatgc
    17821 gtgtaatatt tatccatccc gcgttcgtgc tgattttcag agcttggcgg cgggcaacta
    17881 tgagggctat gaatgggagt gtcacaatgt cgacagtccg ctccaacaac tgacattgct
    17941 tgcggggctt gccgcgcttc atgatctggc gcgaaaagcc gcccacgatg actgattttt
    18001 ttgaatccat ttgggactgc aatatattcg cagtcccttt tttatgccat gcgggttttc
    18061 gcgcgccgcg ccggcctatt tcattctccg cgattttcgt ttatagtatt ggtaattaca
    18121 acgggtctcc catgaaaaaa gattttaaaa aaataacttt aaagaacggt ttgcgggtgc
    18181 tttttgtgaa ccgaccggaa agcttggcgg cttccgttct tattttagtc gaggcgggtt
    18241 cggaatatga aacaaagcgg atcaacggac tctcgcattt tttggaacat atgacattca
    18301 agggaacggc gaaccgtccg cggcccatgc agattgcgga agaacttgcc gcgctgggcg
    18361 cacaatcgaa cgcgttcacg tcgcaggaat acacgggtta ttgggcgaaa gccgaaagcc
    18421 ggaaattgcc gaagatactc gagatagttt cggatcttta tctgaatccg gtttttagtc
    18481 ctgatgaaat agagaaagaa agaggcgtga ttatccagga gcttaatatg tacgaggata
    18541 cgcccgcacg gaaagtgcag gatatttttc tttcgcttct ttacggcgac cagccggcgg
    18601 gatgggatgt cggcggcgaa aaagaaataa taaaaaaact taccaataat gattttgcgt
    18661 catatcggaa ggaacgctat gttgcgccgg gaacgctcgt cgtcgtcgcg gggaagttcg
    18721 acgaaaaagc ggcgatggcg cagataaaag attatttcgg aaaacttccg cgccgtcccg
    18781 taaaatcgaa aacgaaaacc aaagagagcc agaagaatcc gcaggcgctt cttaagttca
    18841 aaaaatcgga gcagagccat tgcgtactcg ggttccgcgc gttcgatctt ttcgacaagc
    18901 ggcgctatgc aatcgaggtg cttgccgacg ttctgggtgg cgggatgagt tcgcggcttt
    18961 tcaagcgcgt gcgcgaagaa cttggcgcgg cgtattacgt gagagccgat gcggatcttt
    19021 ttctcgatca cggcattttc gccgtttcat cgggcgtgga tcacgaaaaa atagatatcg
    19081 tcatcgccgc gattcttgaa gaatgcggaa aattgaagga cgagcttgtt cccgcagggg
    19141 aatttcaaag gacaaaagat catcttatcg ggaatctcat tctcggactt gagacgtcgg
    19201 atgaactggc gagttattac ggcggacagg aaattcttgc gaaagcgatg cttacgccgg
    19261 aagagctgat aaaaaaaata aatgccgtga cgcccgaaga cgtgcggaac gtcgcgcgcg
    19321 caatcataaa agacagcggt ttgaatttcg cggtcatcgg tccgtataaa aacgaagagc
    19381 gattcgggaa actgttgaag ataaaataag ac
//
LOCUS       DAIDDT010000007        17337 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_7_length_17337_cov_5.861298, whole genome shotgun sequence.
ACCESSION   DAIDDT010000007 DAIDDT010000000
VERSION     DAIDDT010000007.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17337)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17337)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17337
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7_length_17337_cov_5.861298"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    2018..2027
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4521 a         4014 c         4120 g         4672 t
ORIGIN      
        1 gataatataa gcaaacctat cgcgtccgtt cccgtatctc tctttgtatt ttttcgatga
       61 accgttccgt tttcatcgcc gtttcttccg cggatgccgg ctccgatgac gataatgatg
      121 cggatttccg gcttcgcacg ttcaccattc cttcatgttc ttctttttcg ccgacgacaa
      181 ggatgtaagg gattttcatc atctccgcgg cacggatgcg tttgcccaat gattcgtcgg
      241 gttcgctcca ttcggcgcgg atatttttcg cgaggagcgt ttcgtatatt tcttttccgt
      301 acttcgcaat tttttcgctg accgggataa cggtgacctg cgccggcgcc agccagacgg
      361 ggaacgctcc ggcgaagtgc tcgatgagta ttcccataaa tcgttccaaa gaaccggaaa
      421 tcgcccgatg aatgacaacg ggccgttcgc gttcgccttt ctcgttcacg aacgaaagat
      481 cgaatcgttc gggaaggtta aaatcgcact ggatggtcgc gagctgccac gcgcggccga
      541 tggcgtcctt gaacataaaa tcgagcttcg ggccgtagaa tgccgcttcg ccttcaatgc
      601 gcttataagg aagtccgtcc gcttttgccg cttcttcgag cgccgtttcc gccgcattcc
      661 agttttcttc ggtgccgagg tactttgatt tatcatcgcc gcgcaccgat aagctcaccc
      721 agtaattttc gagcataccc atcgtcgcgt aaaattcctt gatgatgccg gcaatggttt
      781 tcacttcgtc cccgatctgc gaaaccctgc agaaaagatg gccgtcatcc tgcgtgattg
      841 cgcgcacgcg cgtgaggccg ctcaactctc ccgatttttc gtcgcgatag actgttgcag
      901 gttcgaaata gcggacgggc atgtcgcggt agctgaactg gttgtcggca aaaagctgca
      961 tatggtgcgg gcagttcatc ggcttcatga tgaatttttc ctccttgcct tgcacgcgga
     1021 acagttcgtc gccgaatttt gcggcatgcc ccgaagttat atacagtgat tctttcgcga
     1081 tgtggggcgt ccctacgcgg gaatatccct tgtaccgatg gaggtcccag agataatcct
     1141 cgatggccct gcggataatc atgcctttcg gctgcataag aggaagtccc gcgccgataa
     1201 gatcggagat ggtgaaaagc tttaattgtt ttccgaggac gcggtggtcg cgttttttcg
     1261 cttcctccat gagcgcacgg tattcttcca gttctttctt gcttgcgaat gcgagaccgt
     1321 agatgcgttg gagcatcgga tttttttcgt cgccgcgcca gtatgcgccc gcgatacgat
     1381 cgagcataaa agcatcagcc ggaatttcat tcgtggtcgc gatatgtccg ccgcggcaga
     1441 ggtcggtaaa aatagccgcc gattgccgat tatcagccgc cgacagaagt gttttataga
     1501 tggaaatttt ctctcctttt tcctcaagtt cttttatcag atcgagtttg aacggctgat
     1561 ctttgaatat tttttttgct tcggtcacgc tcaccgtcgt accctcgaaa acgagatttt
     1621 tattcacaaa attccgcatc gttttttcga gctctttaag gtcggccgga gcgaccgctt
     1681 gcgggagttc aaagtcataa taaaatccat gctcgataac cggtccgatg ccgagttttg
     1741 cgccagggaa ttttttaatc actgccgcgg caagaagatg cgccagcgaa tggcggatat
     1801 actccagttt ttcggtttcc tccgttttcg tttccgcttt catgatattt tattctaaaa
     1861 caattccgct ttttttgaaa gcagtccgtt ttctttcttc cgttctgtca tcgtccggct
     1921 tggccggacg atccacagtt tgaaaaatcg acataattct tattatgtcg tcctcgcgaa
     1981 ggctcgccct atggagtggc ggtttcgcct actccatnnn nnnnnnnggg cgacgatcca
     2041 tgctttgaaa aatttaagaa ttaagattga aagcatgggt cgtctctcgg agtttaccct
     2101 cggcaacgat cgaggaggga taacgcagac cggattccat tttgaattcg catccgtgtt
     2161 ataataaaat catggaaaaa attcaagaaa agagtggctt ggttttttat gaaaagggac
     2221 cggctctgtc agaaatgcga tctttcttcg cattgcggga aaacaaaaat cagagcattg
     2281 atcgtcgcgt aacgtcgctt catggtttgt cggtattttg tacggggcag gcaatgctga
     2341 tcaccgtgct ttcgctcggc ggcgccattc tcggggcgac ggcgctcgcg gggtttctga
     2401 tagtctatca gatcagaagc gcgaccgatt tcgcagattc ggcgaaagcg gtgtttgctg
     2461 ctgacgcggg cacggaatgg gcgcttgaca gctatttcaa tcccaatgac ccgccggcac
     2521 cgacttcgtc tttgccattt tcaaatgggg cgtcaattgc cgtagtggaa tgcaacgacg
     2581 gttcaggcgt gcaatacccg ttcggacctt cccctgccga ttgcaatgat tcaaatccga
     2641 aaatatcatt atcatcagga tacgcgcacg tggtgggagc ggcggctggt acgaggcggg
     2701 catttaggga aatgtttaac gcgccgcaat aagaataaga aggtgatggt tattaaagca
     2761 ccgacaggta tttttgttat tttcttattt tttgtttctg tcatcctcga cttggtcagg
     2821 gatccacgct tttgaaaatc aaagattaga agcatagatc ctcgtcgccg tgagggcgac
     2881 agaaaaagaa caagagagaa taataaggca tattacttta tatgaaatca tctgatatca
     2941 ctgaaccacg ggcgcgtgag cggatcgcgc ggctcaggaa ggccatcgac catcatcgtt
     3001 atctttatca cgtccttgac cgcgaagaga tttccgatgc cgcgcttgat tcgttgaaga
     3061 acgaactttt cgacctcgaa aaccggtttc cgcatttgat aacgtccgat tcgccgacgc
     3121 agcgggtggg aggcgcgccg ctcaaggaat tcaaaaaagt gcgccatggg acgcggatga
     3181 cgtcgttcaa tgacgctttt tccgaagagg atatggcggc atggttcgag cggctggagc
     3241 attatttaac gccgcggacc aaaacggaaa gtcgtccgcg cggcttcacc cgatccgtcg
     3301 gttccgctga atctgcgccg atgttctatt gcgagcttaa gatcgacggc cttgccatcg
     3361 aacttgaata tgaaaacggt gtttttgtcc gcggttccac gcgtggcgac ggcataacgg
     3421 gggaagacgt cacgcagaat cttcgtaccg tcgatgccat tccgttgcgg ttttcggacg
     3481 ccgacactgt tgcggcgaac ataaaaaaag ccggccttga tccgaaatca tacatactca
     3541 atccccgccg tcttgtcgtt cgcggcgaag tttttctcac taaaaaagaa tttgcgcgca
     3601 tcaacgccga acagggggaa aaaggcgcga agccgtatgc gaacccaagg aacatcgccg
     3661 ccggttcggt gcgccagctc gacccgaaag tgaccgcgcg ccgccggctt gattcttttg
     3721 aatacgacat cgtgaccgat atcggccaga cgcgccatga cgaggtgcat gcacttttga
     3781 aggcattcgg tttcaagacg aatccgaaca acgggctggc gaaagatatg agcgagattt
     3841 tcgcgttcag ggatcattgg gcgaagcagc gcgagcggct tgattacgag atcgacggca
     3901 ttgtggcaat cgtgaacgac aataccgttt tcgcgcgggc cgggattatc ggcaaagcgc
     3961 cgcgcgcggc gatggcatac aaattctctc cgcgcgaagc gacgacgatt gtggaagaca
     4021 ttaaaatcca ggtcggtcgc accggcgttt tgacgcccgt tgccgtgatg cggggggtga
     4081 acgtcggcgg cattacgatc acacacgcga cgctccataa tgccgacgag attatgcggc
     4141 tcggcttgaa gattggcgat acggtgatcg tttctcgcgc cggtgacgtg atcccgcagg
     4201 tgacaaaggt tctcgccgat ctccggacgg gacgcgaaaa aatattccat atgcccgacc
     4261 ggtgtcccgt ggacggcgcg aaggtcgtgc gcgacggtgt cgcgtatcgc tgttcgtcgc
     4321 ccgtctgcgg cgcgcggcac cgcgaatcgc tgtatcattt tgtttcccgt cccggattca
     4381 acatcgacgg cctcggtccg aaaataatag accggtttct tgatgaaggg ctgatctcag
     4441 atgccgccga tatgttcacg cttgaaaaag gcgatatcgc ggcgctcgcg cagttcggcg
     4501 aaaaatcagc ggagaatatc gtgaacgaaa tcgcggcgaa aaagaaatta ccgctcgcgc
     4561 ggtttctgta tgcgttgggc atccttcatg ttggcgaaga gacggcgaat gcgctcgcgg
     4621 aacattatga tattatgaaa tcacggcacg ccgctatttc gataacggat attttgaaga
     4681 agttccgcga tggttcgctt gaagatttgc aaaaaattcc ggatatcggg ccgaaagtcg
     4741 cgcagagcat tcatgaatgg ttccgcgaac cgaggaacgt gaaatctttg gaacggctgg
     4801 aggaagccgg cgtcatcgtt acggtcgcgc cgcgccgcgc cgcgaacgcg aagtttgccg
     4861 gaaaaacttt cgtgttaacg gggagtttgg catcgttgag ccgcgaagaa gcgaaggaaa
     4921 agatccgcgc gctgggcggc gacccgtcgg gatccgtaag cgcaaaaacg gattacgtcg
     4981 tcgcgggttc cgaacccgga tcaaaatacg aaaaagcgaa acgatggggc gtggaaattt
     5041 taaacgagaa acaatttttg gaacttttga aaaatcattg aaacgggttc cttgcgtttc
     5101 atattttctt tgactttgcc cgtttttcgc gctttaatga gtctatgttc cgcaaaaaga
     5161 tcggcattga tttgggcacg gcgaatacgg tggtgttcgt gcccggtagg ggattcatca
     5221 ttaacgaacc gaccatcgtt gctttgatgc gccccgataa taccgtgctg gcagtcggaa
     5281 gcgaggcgaa agaaatgatc ggcaggacgc ccgatgagat cgtggcatac cggccgctca
     5341 aggacggcgt catcgctgat tattacatta cgaaggcgat gctttcgtat ttcatttcaa
     5401 aggcgacggg ctggctgaat atagtgaagc ccgatgtcgt tatttcggtg cccgcgggca
     5461 ttacgcagac ggagcggcgg gcggtcatga acgcggcgcg cgaagcgggc gcggggaacg
     5521 cgtttattgt ccgcgaaccg attcttgcgg cgctcggcgc cggcattccc atcaacgcgc
     5581 ggtcgggaaa tatgattttg aacatcggcg gcggcaccgc ggaacaggcg gtggtcgcgc
     5641 tgggcaatat cgtttcatgg aatagtttgc gcatcgcggg gaacaagttc gaccaggcga
     5701 ttatggatta tatcaagaag aaatatgcgc ttgcgatcgg tgaacagacc gcggagaatg
     5761 tgaagatcga aatcggttcg gcgataccga cgaaagaaaa actggaatac caggtgcgcg
     5821 ggcgcgatct tatcacgggt ctgccgaagg acatcgttgt caattccaac gaaatggcgg
     5881 aggcgctgaa tccgctgctg ctcgatatcg gcgcgtccgt ccagcaggta ttcaacgaaa
     5941 cgccgccgga gcttgttgcg gatatcatgg aaaaaggaat tatcctttcg ggcggcagcg
     6001 tccagcttca ccatcttccc gagtttatgg agcggtatct cggcgtgcag gcatatgtcg
     6061 cggaggatcc gcttttctgc gtcgcaaaag gcaccggcct tattttgaac cacttggaca
     6121 cgtataagcg gacgttgttg aacaagagat aaacaagcgg agtaaaaagt ggataacgaa
     6181 aagtgaaaag cgcaggtatt cgcctcgcga attcatttat tattcatttt tcgcttttcg
     6241 ctttttactt ttttccatgc tcatcggaca taaaaaaata atcgaagatc ttgaggcgct
     6301 cgttaaaaaa gacgctttgc cgcacgggtt tatttttacg ggtacggcaa tggcgggaaa
     6361 acggacggcg gcgctcgcgt tcgcgaattt tttggagtgc ggcgtttttg cgccgccggc
     6421 gggcgatgcg gttttgcggg atgcgaaaat catcgaccgt gcgtttatga aactgctcga
     6481 tcccgatgcg tcgggaaatt ccatcggcat tgatgcgtcg cgcgaaataa aaaattttct
     6541 ctggcaacgg ccgaacgcaa gcgcgcgccg gacgctcatt atcgacgatg cggcgatgat
     6601 gacaaacgag gcgcagaacg cccttttgaa agtgaccgaa gaaccgccgg cgtcgtcgct
     6661 ccttattctt atcgcgtccg atgcggaggg attgcttccc accattcttt cgcggctcca
     6721 gaaaatttat tttggaacgg ttccggaatc cgacatcgcg gcatggcttt caaaagaaca
     6781 tggcgttccc gcggcaaaag ccgcaggagc cgcgaaacaa tcatccggga agccgggttt
     6841 cgcatggcgg ctgatattcg ataaaacgct tgcaaaaaat atcgcgtatg ccgaaaaatt
     6901 cctgaattcc acggaagcgg cgcggcgcga tatcgtgaaa aaaataatcg agccggatga
     6961 tttcaatatg cgcgtttttc ttgatgccgt tatatttgtt cttgcgcggg ataattcatt
     7021 ctccaaaacc ttgccgcaaa gttcagcggg gcaaatgaaa cgtaaaatcg ttctctggca
     7081 taaaacgctc gcgctttacg ggaagatcgc cgatttcagt ttgaatccgc gattacagct
     7141 ggagaattta ttgatggagt gatgagataa gcgacgagag gtgagtgatt agtaatgctg
     7201 tccgaaaact gccccctctc tgtcatcgtc tggcttggtc ggacgagatc catgcttccc
     7261 atccggcgca atgcttattg cgtcgtcccc tcgaaggagg ggatccatgc tttcaatctt
     7321 ttttctttat ttttttaaaa tgcgggtcgt tcgtcttcac ggacgatgac aaaaaaataa
     7381 aatatccggc caagccggac gacagagggg aagcgttcaa acagctttac ccggatatga
     7441 caattcaaaa taaaaccatt acaataacat tatgaccttg tcggaacaat acataaagga
     7501 tctcgaaacc gcgcttacgg cggcgacggt gaaactgaag caggattttg cggagatacg
     7561 cggcaaccgg ccgtcgccgg atatggtgca gaatatttcg ctttcgcttt atgaccagcg
     7621 gatcacgatc cgcgagctgg gctcgttgag catccttccg ccgcggacga tacagatcgc
     7681 ggtgtgggac aaagatgccg ttgcgccggt cgtgaaggcg attgaagccg cgcaccttgg
     7741 acttacggcg acgaatgacg gcaatactat tcgcgcgacc ctgtcgccat tgggggacga
     7801 gcggcgcgaa gagatgatga agcttgtaaa aaaaatggcg gaagccgtgc gtatccagat
     7861 ccgcggcaaa cgcgatgatg cgatgaagcg gatcagggac ggcgagaaca acaaggaact
     7921 gacgaaagac gacgcatttc gcgccaaaga aaaaatgcaa aaagcggtcg aaaaagcgaa
     7981 tggcgatatt gaatcggcgg tggaagggaa gattgcggaa ttaggggaat agacgtttca
     8041 aatatttttt actccatagc atccaacagc acgtacaagc cgtttacatc ccgccatatc
     8101 cgtatgccat cgggctcggg tttaatcgcc aatttaaaca tatttgcgcc acgggccata
     8161 ttttaaatat aagtcaagcc gatgtttttc taagggttct tttgcggttt tgagcggtgt
     8221 ccccaccagg aatcgaacct acgaccatct ccttgtcatc ggcagggagg atagaatgaa
     8281 tgtgccccgt gtaggaatcg aacctacgac catctcctta aaagggagct gctctaccaa
     8341 ctgagctaac ggggcattaa gggcgcattt attatagccg agcgaaccga tgagtaaaat
     8401 ttttatttta ctaaagggag ctgctctccc gcctcgtcga gctatacgca cgatgtaagc
     8461 aaataaataa tagaggaaaa cgcgacggtt ttcaaccccg ccggaatggt ttatacttta
     8521 tcgtaatgaa cgaatcttcc ctttcgcccg ccgcggccaa tagcgaaccg cctgcgcccg
     8581 cactgtgtcc cgcgtgtcat cagccggtca agccggaatt ttatttctgt ccgaactgcg
     8641 gaaaaaattt acaggaaccg ccgctttcgg tatcggtggg cgcgcagacg ggactttatt
     8701 ttatgagcat cattacgccg ccgatgtgtt ttttgacgat cggcgcgtgg cacggcatgc
     8761 aatatctgaa atcggccgat ctgcgcgcga agcagatcgg catcatcgcc gtcgtcctga
     8821 tggttgtttc aaccgtggtt acggtctggc tcgtttacgc attcacggtc caacttatgt
     8881 cattggtatc gtctttgagc ggagggcttt tgggcggatc gggaagcgga ctttaataat
     8941 tttaattcgg atgattggcg gacaacctgt tatttttttt gatttcctcg ctttttaaaa
     9001 ccttgaattt catttgtaaa taagccataa tgcatcaaga cgttttttta ttgcgggatc
     9061 gtctaatggt aggacagcgc cctctgaagg cgtttatctt ggttcgaatc caggtcccgc
     9121 agcagaccgc aaaacggttt aggatcactc gtggttttct gtggatgacc tcgatcaatg
     9181 aggacttgct gaaccttatc ccaaagacgc ttctcgataa gcggagtatg gcgaccttcg
     9241 taagtttctc ccttatatat aaaaacaccg taataaaatg ggttggctag gatgaagcga
     9301 actctgtcct tatgaagcgg attgccattc gtgcttttga cgccctgttc aaaaagaaaa
     9361 cgagaaatat cttcaaggcg agatttacct aagacgtaca actcgaaggc ggctctgata
     9421 gctttggatt ttcgacgatc gacgacaatg gttttcgcgc ggatatcatt cagatatccg
     9481 acgggagcag taccaggaaa atccccgtgc cgcgctttct gcctcagccc tctgctcgtg
     9541 ttggaactca gatcgcggat gaactggttg gccatgccga actcaacgga catcatcaag
     9601 acattatcgg tcggacgata gctcttatct ggcatgagga tatgttcgat cattccctgt
     9661 tggagcatcc aactgatctg gccgccgtcc acgggattgc gggctaacct atcgagcttc
     9721 caacagacga tgcctttggc ttcgcctttc gccatacgat ggagcatctc attgaagacg
     9781 gggcggccag gcatcttagc agacttcttc tcgacgagtt cctcggctat ctcaatatgc
     9841 tcgtctctgg cgagttcgcg gagttcaacg agttgggctt cgatagacaa aacctgcttg
     9901 tcttcgacat cggtgctttt gcgggcgtag aggaaatagt taggcataaa tggaagtgta
     9961 gacttattat tctctataca cttatcttac gacaggcgat atgatatttg caagggcatc
    10021 aatgaacaca ttatctaaac ggtttggcaa gaaagtcaga aaaacccgcc ttgataaagg
    10081 tctatcccag ggcgacattg cacggcggct gaaattgcat cgctcttaca ttagtgggat
    10141 cgaacgagga gtccgcaatc catcactagg cgtagttgaa aagattgcta aagcattagg
    10201 aatttccgtt cgtgaattac taaaataatt ttttattatg ctagatagat ctataacaag
    10261 tttggcgttt ctcaaagtaa attgggatga aagacatagc gatcacatag atagctttct
    10321 tcctcttttt tctaacttaa ttcatataaa gcattatgaa aaaattgagg tagatacggt
    10381 aaaagtagat tttgaaagcg agtacggatt atctattccc catcatccca taatggctat
    10441 attgggtcgt ctcaggaaga gaggcttcat tacaaaaacc gctgaaggat ttttaccgaa
    10501 ctacactaag attgacggcg gtaattttga aacaatcagt gcagatcaac gacgtaaatt
    10561 aaataaactt attttatcga tcatcgactt cgcaaaaaaa tacgatgtta ctcttgaaga
    10621 gaaagatgcg gaaaaagcac tattaggatt ctttagagaa aatgaactaa gtattttata
    10681 cgctacggac gaaggagaga tactccccga tgtagaagtt gataaatcaa ctaagttttt
    10741 agttgcaaaa ttcatccatt cggcgaaaac atctgaacca gaactgtttg ggtttattgt
    10801 cgatattgcg atagggagtg ctctggcggc gaccgtagta tatggaaaag atttagcaga
    10861 gcatttcggg ggtaggatga aaaacctaaa cctatactta gatgctgggt atatattctc
    10921 tctattagga atggatgcca gagaaagcaa gcaagcgtat gaagaactta ctcaaaatct
    10981 aattaatgat ggcgctaaga taaagatctt tgaacatacg tatgacgaga tgatggctat
    11041 cttcagtaat gccctgcatt ggatgaacca gaaagactac gagtggagta aggcgagtag
    11101 agttttaaga tacttcatca caaatgattt taccgagaca gatgtcgaga tgtttattgc
    11161 tcacgtgcca caaattttag aaaagtttaa gattgaaaaa gttcccaagc ctgaatatac
    11221 caaaaatata gaatatcaaa ttgatgaggt tgcgcttaga gaaataatag ttgccgccta
    11281 taaaacagat ccatattttg atgaaaatca aaaaggagaa actataaata aagatataga
    11341 gtcggtctat tcaatatgca aattaaggca gggcaagata gcatttacga ttcacgacgc
    11401 ttcccatata ttcgtaacga caaataagct cttagccaag ctcagtagtc gagttcaaac
    11461 tagtgaaagt aatccatttt ctattcctcc gtgcgttaca gacgtattca ttggaacgct
    11521 tttatggctt caaaatccag ataaagcagt gacactcaat gaaaagaaaa ttatcgccga
    11581 tgcctatgcg gccattcaac cagatcaaaa tctagtaaaa aaatatttag ctcagataga
    11641 aattctgaaa aaagataata atatttctga aaatgattat ctaattctta ggacatctag
    11701 ggtggctttc aatcttttga gtgaagcaac caaaaatgat gttaataatt ttgtgcctaa
    11761 aacaaccacg caaattctag atgaaataaa caaagaacac atttcatcat taaaagaaga
    11821 ggttgaaaag gaaaagacat ctcacgagaa agccctagaa tccctagaag agcgcgatgt
    11881 aacaatcaaa caaaaagatg agcaggcaga aaccctgaat aagaaatacg aaaagctttt
    11941 ggattttgta gcaaattctc taacttgggt aatcttccca ataatagtag cgggattaat
    12001 tatcgcgcta ataataaatc aatttccttc atttattcct aatctcttgg tacggatcat
    12061 agcttttgtc ataataagca tactgaccct actgggtatc agtataaaaa atacgagggc
    12121 gtggctaaat aaaacaataa aaagattaat catagaaaat attctagaaa agtagtctat
    12181 taatagttaa aaagatctcc ctccaaaaaa tcttcggctc gaacatactt cttaaaaacc
    12241 gtcgtatcga atttatcccc accccaccat acgcttcgct ccgcgaagcg cgcgtgaatt
    12301 tttctgaaac tgacctaagt tttatagctg ctcatctggc ggacgttgcc cgaacgtatt
    12361 ttatggcaaa ttcctgatcg ttttacccct tcaaataccc cttcaattgc ccttcgccaa
    12421 tttgaagggg tagcaaactc ggattgcccc gtatcttctg gcttttttga cccactgact
    12481 cgcaaatcac aagtcctact tcactctgtt ttaccccagc attcacccca gcatgcgcct
    12541 accctcggaa tgatggggta gcaagtccag atcaccctgc aaactctcgg ctttttgagc
    12601 cacctatcat caaatccaaa gtcctacttc actccgctag accccctccg aaatggtgtg
    12661 ctcagcccct cggtctgctg acctcggggc ttcgcgtatg gcgcgcccaa attcctcccc
    12721 cacaaaaaac gctcgaggcc gcgtggccga agagcggtta aatcgaaacg gttttaattt
    12781 tctttagctg atttgtatat tctcctgata aactgcagtc ttccgcacct tgcaaaatta
    12841 catccatgta ttcctgacta ggtaaaccag acttcctatt agttccataa tatacaatag
    12901 cttttagagg ttgatccccg ttcgtaagga cgttcaactc tttccgatta taggattttg
    12961 ggaatccttc ataccgatca agggttataa gatttgcttc tgagatctca aacaatccac
    13021 cactaaccat ctcaccacga atctccacaa tattcgcccc agcacccttc catttcgatg
    13081 aatatccatc aaaaataagc ttgtagtcag ggaggtcagc tctgcctaga aaggtacttc
    13141 ctgggcatcg atcactcatc tgtctatggc tcatatttga accataagca aaatacttca
    13201 tcttcggatg ttatctaaaa atgccaaacc ttatggtagg cactatgggc tagcttgcca
    13261 gccctcatag ctatctcatc agctccccaa cccttagtct taatatgatt ctcaaattcc
    13321 tcgataaaag ttttaacgtg cggtaagtta gcaaaacttt ctttgttctt tagtatctct
    13381 attttttcaa gcggcgtttt agcttgtagt ttcgagtttg tatgtaggct aataattagc
    13441 aagtttccga tgttatcagc tacatcagga tcaggcaatt ctccccgttt agagtctgga
    13501 taaaaatgct caacgttgaa acttcgccta aatccttttc tgtccaagaa aaatagctgt
    13561 gttttttgtc cacccactct atcaaaatta tttatgcgat caataacgta actaataagt
    13621 ggtaggtcgg cagaggtgct atatgaaagt tctttgaaat taggctcaaa ctcttcataa
    13681 ctagccttct tgtcttgaag gatcttaata agttcattgc aattcttttt aaaatccttt
    13741 gcattgttaa atttcggagc gaagtccatg tacgttttct cgatttcgtt acccacacgc
    13801 tcacaaatga cattgttaac gaaatggtat ttctcgaagg tttctataag agatctgagt
    13861 aatttcgcca tttcagcgtt gtctccatat ttttcctgat atgcttttac ggccgaataa
    13921 ataatcgggt agggttgcgt aacacggaaa agccttaagg cttcgatact tgaataaata
    13981 tcgtggcaat aatgttgatt tctagagatt cccaaacatc catttgcttc gaaataactt
    14041 ctcagatctt cctctcgccc ctcccgtaag atggcataaa aagcagaaaa atcactgagg
    14101 tcttttacaa aaatatctgg tttgagttct gaagattgcc tctggagatt tttgaaaata
    14161 tctttctttt ggacagctcc ttttctggca actagataat atttcaacat tctcatcatc
    14221 gtggtatctg cgttttctac tatttcatcc cacgtttctt cgatacccgc aatttcggcg
    14281 gagaagaggt ctgcctttaa aagatcggcg gcattaagtt ccaggccacg cgcattcgct
    14341 ctttcaaaaa gatcgaacac gtcaagatcg ccatcgacat ctatcttgat gacaaacgat
    14401 ccatagagct tttttaacaa ggttgatata tcttcaagtt tcttaagcga cgatacccgc
    14461 tcacggaata attcgtatac tggtttgatc ttattaatct gccgtttaac aaatttcttt
    14521 cctagcttca caggaaaatc tcccttccaa tcgtctctga tgatgtactc aaaaatatca
    14581 cgcaccgcca ttgatgcgcc caacctcgct cccgtcattt caccagtagt agaatcgcta
    14641 aaagttaatt tttcggtaat cttattagca agcttatttt cctttatagc aattgccctc
    14701 gcacgacatg caataagtag taaacaaata gttgttatcc tctgttggcc atcgacgata
    14761 gatatctcgc ctcgattcat attttcaaca tcgagcacaa tattacctaa atatatcccc
    14821 tgatctgatg agataacgtc attccataag tcgtccgctt cgtctttacc ccaatcaaaa
    14881 ccacgctggt aggatggaac taaaaagcgg gcgttaccaa tgtttagaag ctgttcgacg
    14941 gtttgtcgtg atggagttat ggccataatt accttctatg ttatcagaaa aaagctcttt
    15001 tggtaaaccg agttcagggg gctataatcg cttcttgccc gcgcaatggg agggctgggg
    15061 caagggcaag aaaccgcttt cgggagggtc agacacacaa agacgcagga tgttcctatc
    15121 agacaggaat agggtcgtaa aacgtggaac taccacctct gagcgcgccc caaaggaggg
    15181 gttgggggag gaatttgggc gcgccatacg cgaagccccg aggtcagcag accgaggggc
    15241 tgagcacacc atttcgaagg ggtctagcgg agtgaagtag gacttgcgat tggcggttcg
    15301 gtggctcaaa aaggcgagga gatacggggc aatccgcacc tgctacccct tcaaattgac
    15361 gcagggcaat tgaaggggta tttgaagggg taaaatgatc aggaatttgc cataaaatac
    15421 gttcgggcaa cgtccgccag atgagcaggg tctcgctctt gcgagacgag cgtttcgctc
    15481 gaactcttct ctcatacccc atcccgtgag ggtggggttc ttgataataa cccgtgcaaa
    15541 ctaaaggata gtagttacct tttcataaag cattggcaaa caatcctgcc tcattctgaa
    15601 tttcgttcca tgattgtggc ccgtgcgggc atcaaaatca acaagtacct tgccctcttc
    15661 taatgctttc aaaaatccat cgaagttaaa tttttgaagc atcattactt tcgtatattt
    15721 gcagtattct tttttgcgtt ccattttcac atctgcttga acataaaaag cattgaggag
    15781 cttggttccg gctttgtgtt ctaaatcatc aaatccccaa tatggctgag gatcaagttc
    15841 tcccaatcct gcccgctttt tgactaattt aagccaatct ctatgtctcg gatcgacact
    15901 tttcgcgtca aatgaaatta aaatcttttt ttctttgcga ttaataacaa ccttaaagcc
    15961 gcgatcactc cgcgattgtc catgaatcgt ttgacggaaa ctcatctcat ttttgccgta
    16021 ctttttatca gcttccccat ggggccatcc gtatagtggt aatagaactt gtggcacaaa
    16081 ttttactgct cgcggcgatg gttcggtatg gaatagggtg gtaagcgatg tagtatttat
    16141 tctttgcgtt tttaattccc attctgcggc gttaggaatt ggcaagttat tttcttttat
    16201 accgagtaaa tcttcaagcg tgtttcctat acctccggca ttacctcgcc gtgcatttgg
    16261 tatccaatcc atcgcggcga tctctttgag ctttgcgatt aatgccggct ttgtataaat
    16321 aacaggtttc tttgccatga ttataaaagc cgagcttgag atttatgctt tttgattctc
    16381 ttgtcagcca tttcgcaata ctcgggcgaa agttcaatgc cgacataatc ccgcttaaag
    16441 tttaaagctg aaattgccgt cgtgccggaa cccataaaag gatcaaggat gatttttgcg
    16501 tttgttgagg aaactattcg atcgatcaaa gctacgggaa aagcggccgg atggggatta
    16561 ttcatttctt gcgtaaattc ccaaacatcg ccgtgagagt ttgccttagt cgccagttcg
    16621 aatttcggtt tggcgataag ataaataact tcgtaagtcg gcaagaaata tccggcgttg
    16681 aaattgagac ctccttttcg cttccaaatg atgatctggc gaacggggaa gccattcatg
    16741 atgtcttggc ggtcttgcaa taagccacct tgcacgcgcc atttatggtt atagaagatc
    16801 gccccatcat cgggaataat tcgcatcatt tccgttaagc aatctctctg ccacttcaca
    16861 tattcatcgt gtggcatgca atcgttgtga tgactatatc ctttttgcaa agcagcatta
    16921 gcccactttc cgccgcgccc atctttcatg ccgttgccag tggaattttt gaggttatat
    16981 ggtggcgaag tgataacaag atcaactgcg ccatcgggta tctgcttcat aattccaatg
    17041 gcatcaccgc ataaaatttt attaacaaaa tcctttgaag atttggtatt tccccgtagt
    17101 tccttttcag ggacaagtac tcggtttttt gcgattatcg attggctcat aattaaaatt
    17161 aagaagtttg tttaacaaga ggaagtttct ctaagatttc atgtacgaga cccaattctt
    17221 tagccttttc tgcgcttaaa atactgcctt catgtaacaa cttgccaatt tccttttccc
    17281 ttcttccagt ttcctttgaa aggatgccta tgtgtttctt taataattct ttaaata
//
LOCUS       DAIDDT010000008        16847 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_8_length_16847_cov_5.763327, whole genome shotgun sequence.
ACCESSION   DAIDDT010000008 DAIDDT010000000
VERSION     DAIDDT010000008.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16847)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16847)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16847
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8_length_16847_cov_5.763327"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    12206..12215
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14857..14866
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3968 a         3932 c         4424 g         4503 t
ORIGIN      
        1 ttattgcctt attttgaaac ccttagtcga aaaccagaaa aaactataga acaccgtaat
       61 cgtgattatc aaaagaaagg tcgaaataac aatctcaccg taggtaaacc cattgtaata
      121 acttggatcg gttgaggtgc tttgtccgta gagtgtcagg caagttgagc tgctaaattg
      181 gaacgccccg ctatcgctcg aactagcggg atttaattct ccgccattca agaccatcgg
      241 gttcgtatag ctgcatgttg tggttgcgtt tgtcattatt ttggtggcgt ctaccgcctg
      301 gcggctccac gccttcattt taaaccccgg cgtcaaggtc gatccaaacc ctatgtgttg
      361 ataactcaaa aataagcaat aaaatccgaa cctcaaaaca atctagctat ggcatgactg
      421 gacggcggaa acgaaccgcc gtatagtccg gcgaggtcga taaaacagta taaaacccat
      481 tgtcccgaaa gggatcagcc gaaaggcatg atgcaaccaa tgggggctcg taattctcac
      541 ttttttcgag tagaatgacg cggggcaatc ttttagattg tcttccggaa agcccacctg
      601 agttgcatcg gtgggctttt acgttaaaaa ataaaataat gaaaataaac gatgcaatag
      661 acctgtattt gggttctcgt catcagaacc ggaaagcaaa aacggttaca caggatttcg
      721 tccatctctc cgatttcatg ttctacataa gggatctcgc ccgcgaggta gaatcaataa
      781 cccttgaaga atgtgagagc tggctggctc tggtgaagaa gttgggatac gacgacaata
      841 ctcggcaaaa gaaagcgata gcgatcaaaa tctttttcga gcatatgttc aagcgtcgca
      901 tccaagtcag cttcgatccc gatctcattc cggttccgcg caaggttttc aagtttccgc
      961 gcgttgcaac cgaagaacag tatgagcagc ttctcgcggc ggtaaaggat caggggaagt
     1021 gcggcgaaat tacctacgca gtccgggaca aagcgatcct catgcttatc cacgacacag
     1081 gggcgagaaa tggcgaaatt atttctctcg atatggatga tctcaactta gaaaagaaat
     1141 cagcgcggat caaaacagaa aaatcgcgcg gggtcgtacc gtatcgggaa atcttttgga
     1201 gcaatgcaac gaatgagcag ttgaaaaaat gggttgcctg tcgagagaag tttatcgcaa
     1261 cgacggaact cgaagaacca aaaactttgt ttttcggaat caaggccgga tgcaacgcct
     1321 catatcattc cgctaaggga agaaggttgc aagtcagcta cgttgcggaa ttattccgca
     1381 aatactcgaa cagggcgaag ctgccgatca ttaacgcaca ttccttccga catctcttcg
     1441 gtcgcgatct ggcgaaaaag aaggcaaaca atttcgtcat ttcgagcatg atgggacacg
     1501 cccgcgttga gagctcgtat ccgtatacga tgctattcgg aaaggacagg gaagatgcct
     1561 accgcagttt acgaggggaa tagttatttt caagcatgtc atagcaaagg agtttataca
     1621 caaccgacgt ttagtacgcc agggggctgt gctatactcc aatggtaact taaaactttt
     1681 aatattttga tgttttccgg aattttttaa aattatgaga gccccggtga gaactatggg
     1741 aatcgaaata atcggataag gaatattctt tctgccattc cggaaaacgc gcgtcttcgg
     1801 gcgtttatcc ggaatccatg attgatgctc tgaagttctt attctttgat ggaaaaaatt
     1861 ccggcacgaa ccggaatttt aaattcgttg tcatcctcgc ggaggcgggg acccgcgctt
     1921 tgtaaatttt cctatctatc gttctcaaat tggtcgagaa gtcacgtctt aagaaaaata
     1981 ttggaagcat gggttatccg ccttcgcgga taatgacaaa agataagaaa ggccatcacc
     2041 gatatggcgg ctttggctac gaaatttcat tttttcataa tttttgatat actccacata
     2101 tcaatatgag cacctatcaa cccaccatcg gtctggagat ccatgccgaa ttaaagacgc
     2161 ggacgaagat gttttgcgac tcgctgaacg atccggacga gcgtcatccg aatgtgaatg
     2221 tctgtccggt atgtcttggt catcccggga cattaccggt cgccaataaa aatgccgttg
     2281 aggccgtcat taaagtaggg tgtgcgctcc agggaacgat caataaagaa tcgaagttcg
     2341 atcgcaagaa ttatttctat cccgatctcg tgaaagcata ccagatttcc cagtacgata
     2401 agccgatcgt ttcgggcggg acgttgcgcg gcgtgcgtat catgcgggtg catctcgagg
     2461 aagatacggg gacgctgggc catggcgatc atgcgagtct ggttgatttc aaccgcgccg
     2521 gcgtgccgct tatggagctc gtgaccgaac ccgaccttcg cagcgcggag gaagtggcgg
     2581 cgttcgcgcg ggagctgcaa cttattttgc gctacctcgg cgtttcggat gcgaatatgg
     2641 aaaaaggcga gatgcggatc gaggcgaacg tcagtctgga tatgggtacg aaggtggaat
     2701 tgaaaaacat taattcgttc aaggcggtcg aggacgcgat cgcatacgaa acgaaacggc
     2761 aggaagaagc gctttcgcgc ggcgaaaaag tgcatcagga aacgagagga tgggacgaag
     2821 tgaaacggaa gacgttttcc cagcgctcga aagaagaagc gcacgattac cgctattttc
     2881 ccgatccgga cctgccgccg ttcacaaccg acgttttcga tatcgagaaa ttgcgtgcgg
     2941 aacttcccga gctacccgcg gcgaaaacgg agcgattgaa aaaagagtat gcgctcaccg
     3001 ccgcgcaggc ggagcttttg gcgggagatt tggcaatcgc tgaatttttc gaggatgccg
     3061 tttcggaatt gcgggcggat acgcccaacg caaagacggc aacgctctat aattatttca
     3121 cgagcgatct ccgcggactg atgaatgaga ccggcgtacc gctgagcgag ataaagattg
     3181 atcccgaaca tctcgcgcat cttgcggtgc tggttgacgg cgcaaagatc accagccgcc
     3241 aggcgaagga tattttgaaa aagatgtttg agaccggcct cgatccggaa gacattataa
     3301 agaacgggaa gatcggtctt tccgatattg gtgaaattaa ggcggttgtt ctcgctatca
     3361 tcgaacaaaa cccggccgcc gttgcggatt acaaaaaagg caagaccgcg agtctgcaat
     3421 ttctcatcgg ccgcgccatg ggcgcccttc agggccgcgc ccgcccggat atgctgaagg
     3481 aggttttcga gaagaattta taagggtgga tatcggtctt tgtttttttg tcgtcctcga
     3541 cccgcggagc aggcgaagcc gccgctccat agggcgagga cgacggagga aaaataagat
     3601 attctcctta cgggaacggc ggaaagaaaa gatagttcaa tgctgatttt taaatgcgcc
     3661 ccaggttcat tttgtctttc tgatctttca cccaccggat tcttttgtct tttttgaacc
     3721 acgtcatctg gcgcttggcg taggcaacgg tatttttatt tatgagtttt tcggcttcgc
     3781 ccggcgaaat ttttccgtca agataatcga tgacttcgcg atagccgatg ccgtcgaatg
     3841 ttttgcaacg ggagccgtat ttttttacca aacttttcac ttctttcaca agccctcctg
     3901 attttttttt cgaaaaatcc gcgcgggcgg gtccgttccc gaacatttcc ctggtgcgtt
     3961 tttttatcct tttttccaaa acttcttttc cgggaaagat accgatgtgc acggcatcat
     4021 agagcggagg accggatttt cgcgtttcgg aaaacggctt ttttgtcgcg actgcgatct
     4081 caagcgcgcg gatcacgcgg cggggatttt tcggatcgac gatatgcgcc gcttcgggat
     4141 cgagcgctat gagtttttga aaaacggccg ggaggccttc tttttttatt tccgtttcga
     4201 gccgtttccg caggcgcgga tcggctttta cctcggggat tgtgaggttg tctatcacgg
     4261 ctttgacgta caatcccgta ccgccggcga gaatgggaag tttcccgcgt ttgaggatag
     4321 catcgattgc gcgcgttgcg tcgcgcttgt aatccgcgac ggaataatcc tcgtccggat
     4381 ttttgatgtc aagcaaatga tgtcgtatgc ctcccgagat gtaatcttct gattgacctt
     4441 cttttttctt ttttttgtat tcttttttct gtattctata ttctgtattc tgccgcaacg
     4501 gctttgccgt gccgatatcc atgccgcgat agatctgccg cgagtccgca gaaacgattt
     4561 cgccgttata ttttttggcg agcctgattg cgagcgcggt tttcccgctt gccgttggtc
     4621 cgacgatggc gacgatcttc tggttcattt caaaagaagc aacgaatgtt tgtttatcgg
     4681 tttattcggg aaaaaacaac ggatttattt tcgccgttgt cagcagttct ttttttaaag
     4741 gcgggttctc cacgccgcgg tcacgaaaga ttttcaacga gtgccatttc gagcgggacg
     4801 gcggcgaacg gggagtagcg catttccgaa taggcgcgga tgagggattt tatgcattgt
     4861 acgaggaacg cggcgtcggc ggctgccgca agttttttga gtgcggcaat ctcgtcgcgc
     4921 gtaagttcct tttcgaacgc ggcgccgagc gccggatcta tttttacgga aaggactttg
     4981 cgcaggtagt ggatgaggtc tttcgtgaag acgacaagat tatagccgtc gtcctgcagt
     5041 tccgccgcgc gcgccaaact gcctttcaaa tcttttttaa cgatatcttc cgcaaaggca
     5101 tgcagcttca ggatgccggt gcggccggtg atgcgttcga ccgccgtgca atcgatattt
     5161 gcctggagcg acgcgatctg gtcaaggagc gattccgcgt cgcgggcgct gccttcggcc
     5221 gtcgcggcga tgagttcgag cgccggttcg tcgatcttta ttttttccgc tttcgccatc
     5281 gccgccagtt ttttcatgat atcggtttta ccgagctttt taaaggggaa acgctgggcg
     5341 cgggatgtga tggtcggagg aagtttttcg tattcggtgg tcgcaagcac gatcacggcg
     5401 tggcgcggcg gttcttccaa ggttttcaaa agtgcgttga atgccgcgcc ggtaagcata
     5461 tgcgcttcgt cgatgatgta tattttatag cgcgccgtcg caggcgatac cgtgatattt
     5521 tccttgaggt tgcggatttc atcgatgccg cggttcgacg ccgcgtcgat ctcgatgaca
     5581 tcgaaagaat tctgcgcgtc gatctgcgtg cattcccggc aggcattgca gggttcgccg
     5641 agttttgcga atacggggtc agtgtgccgt ttttcgcaat tcaaaagttt tgccaaaaga
     5701 cgcgccgttg ttgttttgcc ggtcccgcgc ggtccgtaaa aaacataggc ctgtcccagc
     5761 cggttgttcg tcgcggcatt cttgaggatt gccatgttcg tttcctgccc caggacatcc
     5821 ccgagttttt tcggacggta tttgcgataa agcgcttgtg ccatgtttct attatagcgt
     5881 tttttttatg gaagcgtgga tcctcgccct atggagtagg cgaagccgcc actccatagg
     5941 gcgagcctgc cgcgaggacg acggaaagaa agagcattgt tgtacttctt gacggctatt
     6001 ttatagccgc tcatcgcttg cttttctttt tcgacggatt ttctttccag tgcttgagcg
     6061 cttcttcgag ttcggcaacg gattcgggtt ttttgttcat aaggaacgtg cagtcgggat
     6121 agcgcgtgca ggcatagaac ggcttgccgc ggccgcgtga ttttttcagg acgatgtcgc
     6181 cgggtttttc cttccggtcg ggcgaagcga ggcaattcgg acatttgaat ccggtcttgt
     6241 cccatatttt cgcgatgcct ttgcattgcg gataatcaat gcagccgagg aagaacccga
     6301 aacgtccgcg gacgactttc atcggcttgc cgcagatcgg gcaccgttcg tctttcgttt
     6361 tttcttcaag ctcttttatc ttcgccgctt cttcaggcat ggggagcgtc actttcacgc
     6421 ccggctcggg acacgcgagg aattttccca tgcggccgaa ttttatgatc atcggcttgc
     6481 cgcaatgcgg gcacggcgtc gtcgaaattt ctatctgttt ttcgaccgtc gcttcttttt
     6541 cgtcgagatg ttttttaaac gggccgtaaa attccgcgca cacgtccgtc catttcactt
     6601 tgccttcggc gatgtcgtca agctcttctt cgacatgcga cgtgaatttg atatccacga
     6661 tttcgggaaa attttcgaca agcagatcgt tcacgagcgt gccgatttcc gtaggccggt
     6721 atttcttgtc gattttttcc acatactcgc ggtcctgaat ggtggacaag gtcggcgcat
     6781 aggtggacgg ccgtccgatg ccttcggctt caagcgcttt gacgagcgtc gcgtcggtat
     6841 agcgcgcggg cggttcggtg aagtgctgaa tcggcgttat gccgtcctgc cgcacgtttt
     6901 ccccttgttc gagtttcggc aggcgtcctt cgatctcatc cgccgcgtcg tcgtctttgc
     6961 cttccgtgta gacgcggatg aaaccgtcga atttcacgac ctgtccgttc gcgcgaaaag
     7021 tttctttttt tgccgtgccg gcctcgatgt ccaccgcggt ttgttcaagg agcgcttctt
     7081 tcatctgcga tgcgatcgtg cgtttccaaa tgagatcata gagccgttcg ctgttttttt
     7141 ctttgaggtc gagttcgcgt cccgatgccg cgcggaggtc ggtcggccgg atagcttcgt
     7201 gcgcttcctg cgctcctttg cttttcgtcg cgtagcgccg cggttcggga agccggtaat
     7261 cgcgtccgaa ttctttctcg atgacgccgc ccgccgcttc aagtgcggac gcggccaggt
     7321 tcacgctgtc ggtcctcata taggtgatga agccgttctc gtaaagccgc tgggcgagca
     7381 tcatcgtctg cttcgccgag aaaccgagtt tgcgcgccgc ttcctgctgg agcgtcgaag
     7441 tggtgaacgg cggcgcggga tagcgtttga cgtctttcac cgcaatttcc gcgatgcggt
     7501 acgcggcgcc ttttattccc tctataatat ctttcgcttc ggcttcgttt ttgatgccca
     7561 tcttgccgac cgactttccg gaaatcgaga aaagcgtcgc ggagaaaaaa gtttttcctt
     7621 tttcaggaga aaattgcgct tcgatggacc agtattcctg tttatcgaac gcattgatca
     7681 ggcgttcgcg ttcgacgacg agccgcacgg cgacgctttg cacgcgcccg gccgaaagtc
     7741 cgtagcgtac tttgcgccaa aggaaagggg acagaccgta gccaacgagg cggtcaagta
     7801 cgcgccgcgc ctgctgtgcg tcgacgagat tgatgtccag cgcgcgcgga tttttcaaag
     7861 cttcaagaat cgcatctttc gtaatttcgt ggaaagcgat gcgctcgata ttcttttttt
     7921 ccggtttctt tttatttatc ttattcaagt ccagcgcttg tatgaggtgc catgcaatcg
     7981 cttcgccttc tcggtcttcg tcggttgcga gaatgaccgt gtcggcgccc gcggcgagtt
     8041 ttttcaggtt cgcgacgacc ggtttcgctt ttgccggaat ttcgtactgc ggctcgaaat
     8101 tgttttttac gtcgatgccg gtcttgcttt ttggaaggtc gcgaatgtgg ccgtaggacg
     8161 attccacggt gaagtccttg cctaaaaatt taccgattgt tttcgctttg gtcggcgatt
     8221 cgacgatgac gaggttcata aaatgagtga aaagttaaaa gtgaaaagtg aaaagcgtga
     8281 agaagtgaaa aactaaaaac gaaaagttat gaattaaaaa ttgaaaagag aagtatccat
     8341 aacttttcac tttatcttat atcctcccgc ggtttcctct attatttcct taatgaggag
     8401 aaaactcaat gcctggttcg cggttcgggg ttccagttta gccatcgcgg caattttgtc
     8461 aacatcggcg ccggcggcgc atgcgcggag cgcgttcaaa atcgatcctt cctcgggcga
     8521 ccagccggtt tttttcgcgc cggaaaattc cccgtgcata ataccatatg cttccataat
     8581 atgctccggt ttcgcgacca gctcggcgcc attccttata agatgatgcg agccggcgaa
     8641 gttcggatgc gcaattggac cgggaacgat gaagacgtcg cggttctgtt ccgtggcaaa
     8701 acgcaccgtc gcgagcgcgc ccgaagattc cggcgcttcg atgacgagta tgccttttga
     8761 aagtccgctg atgatgcggt ttcgttcgag aaaacgatag ggcagcgcgt ccgatccggg
     8821 aggatattcc gaaatgaccg agccgccgtt ttccagtatt cttttcgcaa gccggtcgtt
     8881 cgcgcgcggc gtaatgacat ccgcgccgct tgcgagaacg gcgaccgtga tgccgtccgc
     8941 gtcaaggcat ccaatgtgcg ccgccgcatc aatgccgaac gccaatccgc ttatgattac
     9001 aaacccgtgc tttgaaagtt cgcccgcgaa ccgctgcgcc gttgctttcc cttcgccggt
     9061 ggcttttctt gttcctatga tggcgatggg aattttttcg gacgaaggaa ggttgccgcg
     9121 aacataaatg ccaaatggag gatttgcgat ttccttaagc aagggaggat attccggtgt
     9181 gtttaaaagg ataagccgca tgttcgaccg ttccagtttt tcccattcct tttccggatc
     9241 ggggagaagg ggacggttgc gcaggcattt ttggtacgcg cattgccagt cgccgccgca
     9301 ggcgtcgccg agctttttca gctttccgcg gtcgccgttt tcctcacggg cgagcgcgtt
     9361 ataatagagc gcatcatgca tcggttctca ctgtagcgtt tcgcgtgaaa aagaaaagaa
     9421 acgtttaatg tttttataat gattttctct gtcattgtcc gccctctttc tgtcgtcgtc
     9481 cggcttaacc ggacgaccca ttttttgaaa ataaaaagat aagatggaaa gcatgtgccg
     9541 cctgcctgaa tttatcctcg gcggagttta ccccgtactt tgatacggga accctgggcg
     9601 gacgatgaca gacgcatttc aaaattctct ttatgctata ataaaaagcg atgaagaacg
     9661 ggtttaaaaa atacgcatgg ataagtttcg gggcggtttt cggcgcgatc gccattacgg
     9721 gcgcggcgtt ttattttctt gcgggagata tcgcttcccg gtcaagcgcg atagcggccg
     9781 atcggacggc cattgcgcag caggataatt cccttgccgc tttcacggag gtaaaaggcg
     9841 acgcggtcca ggcggcggcg taccaaacgg cgatggatga acttttgccg acgtcgaacg
     9901 gtctcattga tttcccgcaa tggctcaaga atttcgcggc gacctataat gtcaccgata
     9961 atttcgcttt cggcgatatc attacggtaa ttacgcgcta tgttccgggg gaaatcggtt
    10021 tttcccttac tgcgcaagga agcgaaatcg gcgttttctc ttttctggaa aatctcgaat
    10081 ccaaggcgcc cggttttttg cttgaatttg attcagtgaa ttttaccgaa aacggatctt
    10141 catccaaggc cgtcatcggt ggaaagatgt ttttcaaatg acatggaatg taattccgta
    10201 atcgcgccat gaaaaaatta ttcaaaaaat atccggaagg gacgctgatc gtcctcgcgg
    10261 tcattttctc cgcttttata ttctattatt ttatcggggg gatgatagtc gccgcgcgcg
    10321 gcatcgacaa tgttttcacg gtcaataaaa acgccacgga aaacatgacc ttcaaccttt
    10381 ccggagcggc gaaactggat ctgcggggat tggcgcaata agatttttta tatatcgatt
    10441 tttcgtcgtc ttagtttttt ccgtcctcct cgcgaaggtt cgccctatag agtggcggct
    10501 ttgcctattc cacagggcga ggatccatgc ttccgatctt tttttaagtg cgggttatca
    10561 gccaagccga ggatgacgga gaagaaatcg gtttttgaag gcttgatctt tcgtccggtt
    10621 gcgaaatcaa aaatttcctg tttaaatgga gggcatgcgc ggttagctca gtggtagagc
    10681 gcttcgttga catcgaagag gtcagaagtc cgatccttct accgcgcacc atcaaagctg
    10741 tttcgatgca tccgaatgct ggatttctaa atccgacagt atgcgcattt tgatttttcg
    10801 aaaattttga gtatgataaa ccagcatcca cgggcgggcg gctagttcag tggtagaacg
    10861 ctgtcctgat aagacagagg tcataggttc gattcctatg ccgcccacat agttattttt
    10921 ccttcgttgc cgttttgaag acagaggtca taggttcgat tcctatgccg cccacaagaa
    10981 aatttagacc tgccgatagt tcgaaatttc aaaatagcgg aatatctcca tggatccgga
    11041 aataatttat gaggatgaaa attttctcgc gatcgacaaa ccggcggggc ttatggtgca
    11101 tccggcacgg gtgagcgcga aaaaaatggc ggcgggggaa aaagagccgg cgctcacgga
    11161 ctggcttgta aaaaaatatc cgcagataaa gaatgtcggc gatgatcctt cgctgcgacc
    11221 ggggatcgtg catcgtctcg acaaggaaac atcgggcgtg atgcttgttc cgaagacgca
    11281 ggaatatttc gaatatctca aatcgctttt caaaacgcat gcgataaaaa aaacgtacgt
    11341 cgcggtcgtg ttcgggatcc tcaaaaaaaa cgaggggatt atcgacgcgg cgatcggcat
    11401 aaagaacggc acgctcaagc gcagcgtgcg ctcgaaaaaa atggcgaagc cggccgtgac
    11461 ggaatataaa gttgtgaaaa ccatgcggct tcccggcgcg gataataagg aagcggattt
    11521 ttccctgctt gcggtaacgc cgcttaccgg ccgcacgcat cagattcgcg tgcatcttgc
    11581 gagcatcggc catccgatcg tgggagaccc tttgtacggc ccaaaaaaac agccggcatg
    11641 ggctcgtcct cccgcctttt cggttgccga aaaagatagg aggggaaatc ggctgatgct
    11701 ccacgcgcgg tcgttggaat tcgctccgga accggggcgg gtcatgaaaa tggaagcgga
    11761 gacgcctttt ttcctgccat agtccgccct caatcgttat cgtccggatt ttttctgtca
    11821 tcctcgcgaa ggcgaggacc catacttcaa atcttctctt tttctttcta aagcataggt
    11881 tcttagccaa gccgagaatg acagacggga ttgtcatcat ttctttttct ttctctctgt
    11941 catcgtccgg cttggccgga cgacccacgc tttgaaaata aaaagagaag acaggaagca
    12001 tgggtcgttc ctcttcaggg acgataacag ggggaaatga gtaaaagagc cttttttcgc
    12061 ggataatgat gcggaggtac ttatccacag gttcttttgt tgacaggcgg cgatatgcta
    12121 taataattta aattatatga aaggtcgaac aggaaaagat aaaaagtaat taaaaaagat
    12181 gaataaaata agcagaataa aaaatnnnnn nnnnnagtta ggtgggttca cccttattga
    12241 attgctggtg gttatcgcga tcattgcgat tttaattgtg gcagtcgtga tcacattgaa
    12301 cccttcggag cttttgaagc agtcaaggga ttccaaccgc ctttccgata tgtcaacact
    12361 gaaaaccgcg attgcgcttt atcaggcaga tgtcacaatg acgacgccat taggaacggc
    12421 aggtacatgc tataattctt ttggtattaa tgcatcgtgt acttccacta tacctactgg
    12481 agaatttctt tctgttcttg cgataaatgc gtctacgtcg agggttgttg acggttctgg
    12541 ttggattccg gttgatttca atttaatcag ctcgggcgcg ccaatttccc agcttccgat
    12601 tgatccgata aatagtgctt cctatttcta tggttttgaa gcaagtggta cgtcaatgtt
    12661 caagcttacg atgaagacgg aaagcgcgaa attttcggcg agtggtaccg ctgacgttga
    12721 atcaaccgat ggcggttctt tcaatgatac ttacgaaaca ggacctggcg tggctacatt
    12781 gtagtcatcc tttcttttcg caggtgctaa aaaatccaaa atcccccgaa agggggattt
    12841 tgggtgttat gttaatggag cgccgtccat tatggtttca tttctttctg tcatcctgga
    12901 agtcacttcg actatccagg atgatagatt tattctcttt aataattcaa gaaatgcaaa
    12961 atacgaacct ggatcctgga cgcctcgacc aaggcgttga ggttccggga tgacagagtg
    13021 gatggggatt agcatacttg cggactcaaa attctagaat gacagaacaa tcgctttcgt
    13081 cgaaataaca cgatatgcta tcattattcc atgagtattc ctttgccgtt ggcacaactg
    13141 aagaaactgc tggtagacga aagcgttctt acgggtgagc agtttgatgt ggcatccgtc
    13201 gaagcggatc ggaaaggcca gtccattctg gatgtgctcg tcgcgaacgg cagcgttgag
    13261 tctgattatc tgaataatct cattgccagg atattgggcg tcccgcgtgt taatttttca
    13321 catattccca tcgacaatac cgttgtgagg attattccgg aaaatatcgc gcgtgaacgt
    13381 gagacaatcg ctttcaaaaa agaggacaac ggttcatatg acgtcgccat ggcgaatccg
    13441 tcagatctcg aggcgatcaa attctttgaa cagcggcttc atgcgcggat caatccatat
    13501 tttgcgactg agaatgatct gagggaaggt tactccattt atggatatgc gttgggagaa
    13561 gattttaaaa aaataataag agaaaaagct gcggaggcga ttatgagtca ggcgaagaat
    13621 atcaaagaag cagcagctga tattcccatc gtcggcattg tggaaaatat cctttcgtac
    13681 gcgctcgcgt cgcgcgcgtc ggatatccat attgaggtgc tcgaagaatc tatgtttgta
    13741 cggtaccgca ttgatggcat cctgtatgga gttatggagg ttacgaaggc agtacatcct
    13801 gcgattgtgg cgcggctcaa actactcgct ggtgcgaaga tcgacgaaca ctatgcccca
    13861 caggatggcc gttttaggta taaggtaggt aatcagacca ttgatgtgcg gctttctgtg
    13921 attccaacgt tttacggcga aaaagtagaa ttacgtcttc ttgagtcatc gcaaaaacca
    13981 ctttcattga aagacattgg tatcatggag gaagacgaaa aaataatact gaggaatata
    14041 gagaggacat acggtatgat tctttcatgt ggtccgaccg gttcgggaaa aactacaacg
    14101 ctttatgcga tgatgaatat tttaaacaag cctgaggtga atatcacgac tatcgaagat
    14161 ccgattgaat ataatatgcc gtatgtaaac cagacgcaga tcaacgttca ggcggggatt
    14221 tctttcgctt cgggacttcg cgcgttactg cggcaggatc cgaacataat catggtcggt
    14281 gagattcgtg acgacgaaac ggcggacatt tctgtgcaag cagcactcac tggtcattta
    14341 ttactgtcct ctcttcacac gaacgacgcg ccgaccacaa tccctcggct tttcgatctg
    14401 aaagtagctc cgtttctcgt aagttccgtt ttgaacctta ttatcgccca gcgcctcgta
    14461 aggaaagtgt gcgagacatg cgtatattcg tacgatcttg atgaggagat gaaagatatc
    14521 attaaggagc agttcgagga actgggcatc gccactgagc atatgaaaat accgaaagca
    14581 ttttatcgcg gtaagggatg tgccacctgt ggcgagacgg gctatcgcgg tcgtataggt
    14641 attttcgaag tgcttgaagc aacgaatgag ataaaaaaaa taattatgag tccggatttc
    14701 agccttgctg aacttcgcgc gcaggcgcac gctgcgggga tgaagacgat gtttgaggac
    14761 gggttattga aaatagaact tgccattacg acgattgatg aagtaatgag ggtgataagg
    14821 gaataacgtg atgcccatat gagtgagtgt gaatgcnnnn nnnnnnccca cgacccatgc
    14881 tttgaaaata aaaagataag atgaaaatca tgtgccgccc gccggaattt gtcatcgagc
    14941 gcgatcgatg acggatgatg acgaacatgc gttttccatg atgacggata ataatgtata
    15001 ataaaaaaat acaaaacgat ttctcgctac catattctca attctcatat taaattctat
    15061 taactttcat ttcttcccat gctttatcat tattcggcgg tggcggtatc gggcgagctc
    15121 atcgagtccg actttgaggc ggatagtttg gatcaggcgc ttggattcct gaaagagaac
    15181 aacttgcggc cggtatcggt gcgtcctatc aggcaggtga aagaaggctc tttccagtcg
    15241 cttttcggcg gtatttcgat taccgataaa gtttttctta cgaagtatct cgcactgatg
    15301 cttcgggtcg ggaccgacct cctttctgcc atcaatattc tcatcgcgga tttcgaaaaa
    15361 ccggccgtga agaactttct tttggaagtg cgcgaaaacc tgaccaaggg caagccgttt
    15421 tataaggcgt tcgaggcgcg tgcgaaggtt ttctcccccg tattcgtgag tttaattaag
    15481 gcggccgagg catcggggaa tcttgagcag actttcgaga ttctttccat ttccaccgcg
    15541 cgggaggcgg caacgcgggg acgagtacgt ggcgcgctca tatatcctat cgtactcatt
    15601 atcgttgcgt cggcaattat gactttttta ttcacttttg cactgccgaa ggtggccgct
    15661 gttttttcgc aaagcggtat caaccctcct gttttttcgc gcgtcgtttt cgcgtttggg
    15721 ctttttgtcg gaaataatat cgcgatcatt gcgacggcgg tgattctggc tatcggtgga
    15781 ttaatttatt tcttttacaa aacggaaact ggcgcgcgga cgttcagccg gattatgctg
    15841 tggatcccgg gcgtgaatac catttataaa gagcttgcta ttcagcgaat ggcttcgacg
    15901 atgagcgcgc tcacgaaggc gggccttccc atcatccaga cgatcggtat tgccgccgat
    15961 acggtcggtt tgcgggaata ccggtacgcg ctggtgcgta tcgcgaacga aggccttgcc
    16021 aaggggctta cgatcggcga agcgttccgc cgcgaaacgg tatttccgaa aacgcttacc
    16081 aacctcatcg cgatctccga gaaggcgggg catcttgaag aggtgctggg gacgctcgcc
    16141 gatttttacg aagatgatat caattcgagc atccagcaag tgacggcact tttggaaccg
    16201 gttctcctgc ttgccatggg cgtcatggta gccgcgatcg cgcttgccat cattgttcct
    16261 atttatcagt tgacatcatc gttttaaggg aatggtgaat aagcaggcga taggcaatta
    16321 gtatttgtca ttttatggac ctttttcaaa aaaatataac caatcgccgg gcctttacgc
    16381 tcattgagct tatcatcgtc ataggcctta tggtcgccat aagcctattt gtttttccgg
    16441 cgcttttcgg caggcggaac acggccgata tgcaaaatac ggcgcagcag attgcgtctc
    16501 ttttgcgcca ggcgcagaac gattccgtat cggggaagca gggggtgtcg tggggtgttc
    16561 attttgaaaa cggaacgtcg accgcgcctt tttacgcgct tttttcttcg gcatacggcg
    16621 ccacgagcac cgtggcgcat tatgcattgc cgccgaccgt gggatatgtg acatccacgc
    16681 ttgcggcggg atcgtcgctc gatatcattt tttcacaggt ttcggggaac gcttccgctt
    16741 cgacgagcgt cggattgtat cttttaagcc agccgtcggc gcaatcaatc atttcgatcg
    16801 cgtcatcggg agcggtgagc tattaaataa aatatagaat ttagtat
//
LOCUS       DAIDDT010000009        16401 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_9_length_16401_cov_5.696827, whole genome shotgun sequence.
ACCESSION   DAIDDT010000009 DAIDDT010000000
VERSION     DAIDDT010000009.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16401)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16401)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16401
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9_length_16401_cov_5.696827"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    12537..12546
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3775 a         4671 c         3914 g         4031 t
ORIGIN      
        1 agccgccact ccacaggtca aggatccgcg ctttgaaatt ttcgtaccgg gagcatggat
       61 tccctccttc ggggggcaac ggaagggaaa atgcttcgac ggaatcggcg ctgattatag
      121 tgagaagtta cagaataaga aatcgacttc tatggttctt tattgcgcca gccagtgtaa
      181 ccgcttccgc aatttattat tgatcgccga attgcttttg tcgaaatatg cctccaggaa
      241 cggaatgaag taacggaaaa gggcgccggg atggctcgcg aacgccttca tgtccacgga
      301 aaaattgaaa tccgcaagaa tcgccgttat gggaagatgt tcaaatgccg tacccgcagc
      361 gcggaacggc atttcaaaag gcatgccgta cggcgcattg acataaaccg atccgccgat
      421 tttcgatctt ttcactgcat cgaaaaacgc atccattgcg ggctgaatcg tttttttgaa
      481 cacgtgcgcg gcgggatcgg acattgcatt cgcgcaataa gcggcataca gttcgcgcgc
      541 ggcggcggga ctcacgtgaa aatcgcggca gacggcgtca aagatcaaag aaaacgacca
      601 tgcgaatttt tcccgataaa gcaccgcatg cccgccgggc gccttggtaa gaacgaaaag
      661 cgatgccgcc tcatcgtccg cgataataag cgcgagcggc agcggccgga accgcgcgag
      721 cgaggaaaga cgcacttgag gagattccgc gaaaaaaaat ccttcatgcg cggcgaagag
      781 cggcttcagt tcttcgaata gttccctctt tgtgaatata agttcaaaga ggagcgctat
      841 tttcttgccg gaacatccga tgggactttg gaccgcgcga ttatcaatct tgaaattgct
      901 tgcctttgaa gaaacgagaa tcgtatggat gtcgccggca ttgagccgat gcgccgcctc
      961 gctccggcat tgatcgaaaa tcatcgtcat cgcctgcgca atcagattct cgaactcaac
     1021 catcgtaatt ttcccgttca ccaagcgctc ttcgcgcgaa agatcgagcg gcaccggaat
     1081 ggtcgtcgca agcgacgaat ctgcaaccgc cataatcctc cggtacgact tgaaaaaata
     1141 atgcccttcc agcgacccgc gcgcgatttt tcggacgggc gatctgaaaa atttcttgag
     1201 atttattttt ttctccagct tttcgaaaat cagattacga tcttcgtcga tgctcataaa
     1261 aagcccgctc gtctccgtag gcgtaatctc cagaataagg aatttttctt tggggcggaa
     1321 aagattgagt gccacgtttt tattcattct accacgccgg cgataacgca gaggatcgcc
     1381 aaaatacttc attctaaaat tgctcttatg cgccggacac ccgcagaaac cgcttcttct
     1441 tttatgattt ttatttttcc gatttcaccc gtatgcgcga catgcggccc gccgcagaat
     1501 tcgagggaat atgccttttc gaatccgggg ttcgccggcg tcgcggattt cggcccaatg
     1561 gaatagaccg aaacgcgatc gggatatttc tgcccgaatt catgctcggc gccgagtttt
     1621 tccgctgcct cgcgcgcgag ttcgctccgc accaccggca gatcttccga aatcttctcg
     1681 ttcacgatcc gttccgcttc tttcttttgc tcatccgtca ttttcccgcc gtacgaaaaa
     1741 tccatacgga gccgctcgct cgtgatattg ctcccccgct gttttacctc cggcccgagc
     1801 acggtctgca atgctttcaa taaaagatga tgcgcggtat gcaaccgtgt cgtctgctcc
     1861 gaatgatcgg caagcccgcc tttgaaaacg ccggcggatg cggtccggga aagatcgcgg
     1921 tgtttctcca tttccgcatc aaataatttt ttgaactttt ctttatcgac aaatattaaa
     1981 cgtctcgcca gctcttcttc tataagttca aacggaaaac cgtatgtttg ataaataaag
     2041 aatgccttca ccggatcgac ttcattcacc gcaaggattt tatctccttg ctccggagga
     2101 atcatgctaa cgtgtccact ccccataatt ttttcaagtt cctttaatcc ttctttaaga
     2161 gttacgcgaa atttcttctc ttccttttcc atttcatcaa gaatacgatt cgcatgctcc
     2221 gccaagccat gtccttgtgg atacgcagcc aaatattttt ctataaaagc ctgagcgaca
     2281 gaagaaagcg aactctgatt ggaggttttc ccttgagttg ctcctaagcc atctccaaat
     2341 cggatcgcgc ggcgaagcaa gcgacgaata agatatccac gtccgacatt cgacggagaa
     2401 acgccatcag caatcaaaaa aaccgctgct cgaatatgat cgagaattat tcggaactct
     2461 ttttcgttct ctccataaga ttttccggtt tcagcttcta aaactttccg ggcattttcg
     2521 aaaatatcga tattaaaaat atccggatca ttatttgccg cagcggcgat gcgttccagc
     2581 ccgccgccga aatccacgtt gcgctgcgcg agcttttcga aagaaccgtc ggcgcttttt
     2641 ttgtattcca taaaaaccga gtttcctatt tcaagaaaac gtccgcaatc gcagttcgga
     2701 tggcagtgcg ccccgaactc cagcttatgc accggcgtgc cgtccgcggt gaattctccg
     2761 aaatcgtaaa acacttccga atccgggccg ccgggttcgc ccgcgggcat gttcgaaggc
     2821 gcgcccgcgc ggctccacca attttttttg acgtcataag aaaatatcct gccgccctgc
     2881 attcctttct cgcttccttc tttttccgag ccgaaatcga catactttgc ctcaatacct
     2941 ttttgcgcga agagttcctt ccatctttcc gtcgattctt cgtcgcgcgg aatgccggtc
     3001 gcggcatcgc ccgcgaaaac cgtcacatat aaatttttcg gatcgagccc ggcgcctttt
     3061 tccttgtcgg tcaaaaactc gaagaaccat gcaagctgat catgcttgaa ataatcgccg
     3121 aacgaccagt tgccaagcat ctcgaaaaag gtcgtgtgcc ggttgtcgcc gacttcatcg
     3181 atatcttcgg cgcggaaaca tttttgcgcg tttgccaatc gcttcccttc cggatacggc
     3241 tggcccaaaa gatacggcac aagcggctgc atgccgcttc ccgtgaacag cgtcgtcgaa
     3301 tcgttttccg gcaccaagga cgccgacgga ataatcacat gcccgcgcgc tttataaaat
     3361 tcaaggaatc ttttacggat ctcggaagaa gtcattggat taaagattct ctcctctgtc
     3421 gtcctcgcga aggcgaggat ccatgctttg aaaatccaaa ggaagaattg gaagcgtgga
     3481 ttctcagcca agccgaggac gaccgaaagg aatttaacac gatataagga tgataattat
     3541 ccccgccctt tttcattgct cgctatttac accagattat caaacccttc gagtgccggc
     3601 gccttgccgt gttcgcggat tttttcgagc gctttcgcct caatctgccg gatgcgttcg
     3661 cgcgtgacac cgaacgcttt tcccacttct tccaaagtat ggcttacgcc gtcatcaagc
     3721 ccgaaccgca ttttcaaaat atcctgttcg cgctccgaaa gatcgatcat aatttcttta
     3781 ataagatcgc gcaacatggc gcgcgcggtc aattgcgacg gcgaaggatt tttctggtcg
     3841 ggaatgaaat cggaaagcgt ggaatcgtcc tcatcgccga tcggcgtttc aatcgacagc
     3901 acgtcctgcg aaattttctg gatgtgccgc accttatcga tatcaatccc catttcggcg
     3961 gcgatctcct ccacgagcgg atcgcggccg agttcctgga tcagctggcg gcgcgtctgc
     4021 gtatacttcg aaatcgtttc caccatgtgc acgggaatac gcaccgtccg cgactggtcg
     4081 gcgagcgcgc gcgtgattgc ctggcggatc caccaggtcg cgtacgtcga aaatttgaag
     4141 ccgcggcggt aatcgaactt ttccaccgcc cgcgaaaggc cgatattccc ctcctgtatg
     4201 agatccagaa tgctcaaatg cggcgtgcgg ttcacgtaac gcttcgcaat cgaaaccacg
     4261 agccggaggt tcgccttcat caatcgctgc cgcgcttcct cgtcgccttt ctcggaacgt
     4321 ttcgcaagat cgcgctcttc atccttcgtg agcaacggcg ttttcccgat ttctttcagg
     4381 tacatctgca ccgcgtccgg cacgtcgctc ccaaacgcca tttcatcgtc aagcgtttcc
     4441 ttctcgacgg tctctttcga aaaatcaatc agcgcggagg tctcgatcac cttgatgccc
     4501 gctttctcga gacggtcata aatctcctca agcagcgcca cgctgtcttc tattttagga
     4561 aaataataaa gtatctcatt gtcggtcacg aacccgcgcg gacgcccgcg ctcgatgagt
     4621 tcgttgagtt ccgattcatg gatccggaca ttcttctcga atttcgtttc cggttttgat
     4681 tttattttcg gtttatttat cgccggcgcc tgcgtcttta cggcaggatg ttttgataat
     4741 tttaccgccg gattttttgc cggatttttt ttggcgctct ttttaacctg cttcggcttc
     4801 ggtttatgaa aaggtttctt tttgtttatc ttcttggctt ttgttttgat caagcgcttc
     4861 gcctgtttct tttccgcgcg ccgggcgtgt ttttgacgct gcgccccggc tttacgcgcc
     4921 tttttgttct tttgtgatat attcatggag gattctgatt acccattatt ttactttaat
     4981 ttaaagatta tgcaagccga tttcttgtca tcgtccgccc ttgattgcgc tcaatggcgg
     5041 aaaccttccc tttcccgcaa tgcttttttc aaaacgactg ttttgaaaaa agcattgcgg
     5101 gtatccggca tccgtaaatt tttttcggag aaaaacctgg gcggacaagt attccgttcg
     5161 ataagccaaa aacaagccgc aaggatggca gaaagcagaa cccgtctcaa aagcaggctc
     5221 cgtcggcctg cctgaaaaac tgcttttgag acgggttcta aaccaatagc cgctcatcgc
     5281 cgttccctgt cggcaagttt tttaattcct ccaatgcggc cttcaactcg gcgtcattgc
     5341 cgctcgcttc ggcgttcctg accgcaagct tgagaatatt ccttcgctct ttaatatact
     5401 ctttcataag ttcggttttg agcgttttta tttccgcatc cgatgactcg ttcgatgcgc
     5461 ggagaacgat agtgccgatt gtctcatcaa gcgccggatc gtcgctcttt cgcgcaccgc
     5521 ttttcaaaat ctccaaaatt tccttttgcg gcggcgcgag ataatcctcg caatcgtcaa
     5581 aaataccgaa atcgtttttc gaaagcgcgg cggcaaacat ccgctctata ataaggtcgc
     5641 ggcgcgcaac cggcttcggt ttctccgata cttccgcctg tccgcctctt ttcgaataat
     5701 ccggcgcggt cgcaacggaa atttcttccg cttcctcttc caatactttg acgcccacgc
     5761 ccgtgcggcg ggagagttct ttcatccaga aatcgcgttc gacgggactc gcgatatctt
     5821 tcagcttttc caataccgcg cgaagcttca gaagcccttc gcgatcgctc agcgccgcgg
     5881 tatccgtcgg caaatatttc ctgaaataaa attcgggagc gggtatcgcc gcggcaaatg
     5941 tcttcaaaac gttttccgga tccgcctgcg ccgcatccgc tgcgtctttg aaatcaacga
     6001 acgtcgcgac cttcacggaa aaatcattcg cttccgcgag cgcgatcgcc cgttcggcgg
     6061 cgtccgatcc cgccgtatcg ttatcgaaac tcaaaacaag ctcctccgca aaacggtgta
     6121 atgtccgtaa atggtcggcg gtcaaagccg ttccggaact tgcgatgacg tttttaacgc
     6181 cggactgata actcatcaga aaatccatct ggccttcgac cagcaccgcc gattttttct
     6241 cgcggatcgc gtctttgctt ttccagaatc cgtacaaaag tttcgatttc tggaagatcg
     6301 gcgtctcggg actgttgata tattttccca tgtcgccgcg atcgagctgc ggcagaatcc
     6361 gccccgtgaa accgaccacc ttccccatat gattatggat cgggaacatg atccgcccgc
     6421 ggaaacggtc cagcatcaaa ccccgttccg tctttatcgc aagccccgcc tgcagaagat
     6481 cctgcggcgc cgtgcccata ttcagaaaaa acatcgacag cccttccggc tcgttgggcg
     6541 cccatccgat ttcaaattcc tctattattt ccttttttaa tccgcggtcg gcgcaatact
     6601 tttgcgcaat aggcgccgcc gcaaatgctt tctggaaaaa ttttttcgcg gtctcgttta
     6661 aatcgtacaa caaaccgctg aacttatatt cggcgggatt ttcatgtttc agttccacgc
     6721 ccgctttctc cgcgagcgcg cgcatcgcct cgccgaactc gatattctca tggcgcataa
     6781 gaaacgtaaa aatatcgccc ccgagcccgc aaccgaaaca cttccacgat tgccgttcgg
     6841 gcgacaccat gaacgacggc gatttctcgt ggtggaacgg gcaaagcccc ttgaaatttt
     6901 ttccggcggg cgtgagcacc atatatccgc ggagaaattc cacgatgtcc agcttgtttt
     6961 tgatgagttc cgtggtgggc gatgacattg cttcgattat atgtccgcgg acaaacggcg
     7021 gcaaatgccg catgaacaca cccgcgcgtc gttattcgcc cttaaatttc aaccgcaaga
     7081 aatatcatgt tataatggaa aaaacatgaa aaacatattc tatcgctcct tcgtaccgtg
     7141 gatcccgtgg gcggtcggac tgacgcttct tttcggcgcg atgtacggca taagccagca
     7201 ggtgctccgg cagtcggcga acgatccgca gatccagata gccgaagata ccgcggcggc
     7261 cataaacgcc ggcgaaccgg taacggtttt caactcgcag acaaaaaccg atatcgcgac
     7321 aagtttatcg ccttatctcg ttatttataa cgcgagcggc gtcgtcgtcg cttcaaccgc
     7381 tctattaaat ggcgcgccgc tcgcggtgcc gcaaggcgtg ctccaatctg caagcgcttc
     7441 cggcagcaac ggcgaaaacc ggctcacatg gcagccgcaa cccggcgtgc gaagcgccat
     7501 cgttgtcatt cccgtaaaag gggttttcaa cggtttcgtc gtcgcgggcc gctcgctccg
     7561 cgaaacggaa aatcgcgaag accatatcgg gctgatcgcg ctcgccgcat ggttcgtttc
     7621 cctcgccgcg atgttcggaa ctttcgccgt cattgaaatc ttaaagaaga aaagaatgct
     7681 tccggaatac tgacggaggt caaccggtcg ccgcgctcaa taaaaattca tcccgccctg
     7741 cttcagctct tttcttatcg cggcatgatc gggcaccgtc cgcgccacca acgcccagaa
     7801 ttttttcgag tgattgaatt ctccgcggtg gcaaagctca tgaacgacga cgtagtcctg
     7861 aactttctcc ggtaaaaata taatcttata gttaaaattc agatttcctt tccgtgaaca
     7921 gcttccccag cgcgttttct ggtttttgac gctgatgcgg cgataatgaa aaccatacgc
     7981 cttactgaat cgctcgattt tttccgttac aagcgccgcg gcggcgcctt tatgctttaa
     8041 ataatcttcc cggccgtaac gcgcaatggg acttcccctg aactggctga aaaaatgaat
     8101 ttttgaaaac agccacgaag ctttttcgcg gataaactgc tcggcgatgt tttctttgag
     8161 accggccggc gccgtgagca cgaccgagcc gtcgcaatgc accgcaagcc gcatccgccg
     8221 cgcgcgccgg ctctttcgca gcgtgtaaga gactttttta tcctgtaatg cgatctcttt
     8281 taaaacagct ttcatctttc cattgtccgc tccatccccg ccgccgtcaa tccttgctta
     8341 atgaagtttt aataaaacct catccgcttt tttccacatt gaggcgcaaa aattcgtatg
     8401 cgactttcgg cgtacttttt tatcccgccg gaagaaattt cggcttcata tttcgcgatg
     8461 agcgccccga tttgttcctt gtcaattttt tccgccatga tccttattgt tcctcatgat
     8521 agccgatttt cggaattttt gctgcggtaa aatttttatc atcgctatcc gttcggggcg
     8581 gtgtaaatcg taaaagctct atttctcgaa cgtcaggcgc gcaagacgga ggatatcgta
     8641 cgggatgcgg ccgtcgaaat gatcgaagac gggcttgagc gggatcgcgc cgagctcgcg
     8701 catcgcggcg tggatatcgc gcatttcgtt ttcttcgatg ctgccgcggg cgatgtgcgc
     8761 gggcgcgggc ggcggcagat cgccgcgcat ctgtaatttt tccaagtgcg ctatgatcgt
     8821 tccttcggtg aatgaccgcg cggcggcgat ttcgaaaacg gtcttgccgt ttttcacgag
     8881 cgccaacgtt tcgagatatg tgttcggctt cggcggtttc accggcatcg cggcatacgg
     8941 cgtcgccgag ggttcgatat tgccgccgca cgcgcggata aattgccgtt gcatatcttc
     9001 gattttttta aaatccatgc cacggaattt cagtaatgcc tcatccgaca ctgcgcgaaa
     9061 cgcggcatct ttcgccagaa tatcgggatg cacttccagc gcgcgttcgt tcaaaccgag
     9121 caagtgcaaa cccgccagcg tccgcaaacg cgagagcgcg acatagccct gcccgtattc
     9181 gaacgcagcc gaaagatcaa tggccgccgc gtcgagcgac atgccctggc tcttatgcac
     9241 cgtaatcgcc cacgcgagcc ggagcggtat ttgcgtaacg gcagcgagtg tctgtaattc
     9301 gatttctact ttccagtcat ccggttcggc gatgatcgat ctgccggcgc gcgttgcgac
     9361 gaccggcaaa ccgtcatcat gccgaaaccc gacgaccgtt cctgtcgtgc cgttcgcata
     9421 ttttccttcg atgctattct tcgtgaacat cactttcgcg ccgactttga gccgcagcgt
     9481 ttccggcgaa aggcagccgc gcttcaactg ttcaacaagc ttcggctggc cgcggctttc
     9541 cattttgaaa acatgttctt cacctgcaag cttcgcaagt tcccgctcat tcacgcggtc
     9601 aacgtcgcta ttgtgcgaaa aaagtttcgt gatgccgtcg tgcaccgttc cgagccgcgc
     9661 gccgcgttcg agaagtttgc ttttatggcc gtcatccagc gaccccgcac ggagcgcgct
     9721 caaaatttcg agaaatgccg catcttcctg ccggtactgc tccgaaagat agcataccgt
     9781 cggaacaagc gcccgccatg ccgccgattc gtacgcgaat tgcgcgccgc tcgcggccgt
     9841 attcgcaaac gcaagccgcg catcaggatc ttcgttccgc cgcacgatcg gcggaagctg
     9901 aaaaaaatca ccgaccaaaa taacttgcat accgccgaac ggttcctgcg aaccgcgcac
     9961 cgcgcggcag acggcgtcca ccatatccag cgtttgcgcg gaaagcatgg aaacttcatc
    10021 tataataagg acgcgcgcat tcgcgatccg gcgcgcggcg cgcttattgc cttcgatcgc
    10081 gcgaagatcg tgccgcgtaa gcccgctctt gatgccgatg ccgctccacg aatggatcgt
    10141 catgccgccg atatgggtcg cggcaatgcc cgtcgacgcc gtgatcgcgg gcgcgatgct
    10201 ccgctcccgc agatacgcga cataccggtt cacgaggtgc gtcttcccgc tgcccggttc
    10261 gcccgtcaga aaaacatccg cgccggtttt caaaatttca aaagcttcct gctgagtcat
    10321 tgttcaatat tgtaatgcaa atgcattaat aatacagctc acttattaaa atgagattaa
    10381 aaccgcctat tgaacgacaa aactgaacac cttatttttt tatatctcct ttcaattcaa
    10441 aatgataaat gagttgaaaa atttatatgg cgccggtagg atgaaggggt agctagcgac
    10501 aagggtgcgc gacgccagcc atcaatagcc acggcattcg cgcgtgctta gctcggtggc
    10561 ggaggaaaaa attcttattt tctcattcta cgtcccgccg cggttttatg atagcgcctt
    10621 gtcggataat gagcgcagtg gcagagcgtc tcgtttattc atcccgttag agatccttat
    10681 ggacgtttag ctcagtggca gagcgtctcg tttacaccga gaaggtcgtg ggttcgatcc
    10741 cctcaacgtc cacaccaaat aagtctctaa cggaacaagc cttatacgaa atactgacag
    10801 gctcggtctg gacgctttat gaaaaccaat gcttttccca tttattttct tgcaacgcag
    10861 aaactgatcg cactttaaac ggggtaaggg aggaaggccg gggactcgaa tcctcaacgc
    10921 gcgcttttta tatcctgtcg tagcccgctc cggccagacg aaggcgggag tctgctcaac
    10981 agctgcttct cttccgctgg attgagttta tagaaattct ccataagttt cgcgacaaga
    11041 acgggtcccg taccgccggg cgtcggcgta tagaaaacgg gcgactgacc accgtctacc
    11101 ggcaaacgcg cataatcgaa atcccccgac agcatccctg tctcttcatg gcatccgtca
    11161 acgggcgttt ggcgtcttgc atatccgaaa tcgattacgg cggcgccgga tttgagcatt
    11221 gccggcgtga taagccccgc cctgcccgta cctataatca caagatcggc atatttcagc
    11281 aagctgagat cgctcgtact gcgaagaaga acggtttcct tcgcgcggcg catgatccaa
    11341 tgcgcaatcg gccggccgat taaaaacccg aggccgacga tcgcggcgct atgcgccgca
    11401 agatcgtact tttgcgtttc gcaaattaat gcaataacgc ccactgcggg cggaagcgct
    11461 ttcccttgtc ccgcgtaaaa cgcgccaagc gcgcgccctc caagaacgtc gacgtctttt
    11521 tccggcggaa tggcattcaa aacggaatac cggttcaaat gatccggcaa cggcagttgc
    11581 acgatcacgc cgccgcacgt tttgtgcgcc gcgattgctc cgacctcgcg ccgcaatgcg
    11641 tcgcttttta tttcctccgg aaaccggtaa agccggaaat cgacgccgat tcttttcgcg
    11701 gcgttttcct tttgttttaa aaaacttatg gatgccggat cgccaccgac caataccgcc
    11761 gcgagaaact tcttcggctt cggaagctcc aaaagccggt ttaaaatatc attcgcgatt
    11821 tgtttgccgt cgatgatcat aaatgcagat attttttgct tgtcatcgtc cgtgaagacg
    11881 gacgacccgc gcttccaatc tttttaaatt ttcaaaacat gggttcccgc cggagtctat
    11941 cccgagtttt cgaggggcaa ggatgacaga aaatcggata ccgataaaag ggcgcctatc
    12001 tccccatgtc attatcgcac tttgtatttt ttacaaagag tcgtcacctc gcctttcacg
    12061 tttttattcc ggacaagcac gtcgtcaatc aggcgggcga ttattgccat ctctttttct
    12121 ttcatgccgc gcgaggtcaa cgccggcgtg ccgaggcgga tgcccgaagg atccatgggc
    12181 gggcggcggt cgtagggaat cgtattttta ttgacgataa tcccctgcgc ttcaagccgg
    12241 tcgctcgctt cctttccgcc gatgccgcgc gccgcaacgt caaccaaaag caaatgcgta
    12301 tcggtgccgc ccgaaataac gcgccatccc gattttttaa gcgcggccgc gagcgcccgc
    12361 gcgttccgca caatctgccg cgcgtatttt ttgaacgacg gatttagtgc ttcttttaat
    12421 gcaaccgcgg ttgccgccac ctggttcata tgcggcccgc cctgcaaccc cgggaaaacg
    12481 gcgcgatcga tcgatcgcgc gatatcgatc gcgttccgcg ccgcgatctt cgaatcnnnn
    12541 nnnnnnaatc gcggcgcgcg aaaatcatgg cgccgcgcgg cccgcgcaaa cttttatgcg
    12601 tcgtcgtcgt cacgacatcg gcatagggga acggcgacgg atacgcgccg cccgcgacaa
    12661 gaccggcgaa gtgcgacata tcgaccaaca aatacgcgcc gacctcatcc gcaatggcgc
    12721 ggaatttttt gaaatcgatg atgcgcggat aggcggtgaa tcccgcgacc acgatgcgcg
    12781 gcttttcttt tttcgccatg cgccggatcg cttcgtaatc gagcagtccg gttttctcgt
    12841 ccacactata aagaaccgaa ctccagaatt ttcccgtcgc ggaaacgggt tgtccgtgcg
    12901 tcagatgccc gcccatgtcg agccgcatgc ccatgatctt cggcattgga atttttccgc
    12961 actccgccgg tttcggcgga cacgcaagcg caaggcatac cgcaaggttc gcgggcgaac
    13021 cggaatacgg ctggacattc acgctccaat gcgcgggcga cagacggaaa agttcgagcg
    13081 cccgcgcgcg gcaaagattt tcgagttcgt ccacgcattt attcccgccg taataccgcg
    13141 cgccgggata tccttccgcg tatttattcg taaaaacaga ccccaaagcg cggaggacat
    13201 cccgtgaaac gatattttcc gacggaataa gatcaagcgt cgtttcctgc cgctttgttt
    13261 cgtcccgtat caatttttca acaataatat ctttcatgat gttctcattt tatatcaaag
    13321 cgcgcccaaa gaaaaccgcc gtaatttgtt tttatatgca aaccggcgtt ataattttcc
    13381 cattatgatc ctttccgatc gcgacataaa aaaagcgctc aaagaaaaac tgatttttat
    13441 agatccgctg ttcccccgcg ccatccagcc cgcatcggtc gacctgcatc tcggccccga
    13501 ttttctggtg ttccaggcgg aagaacacac gtgcatcgat ccgagagaac cgctcgacca
    13561 tatgatgaaa aaaatgaaaa tcgacgcaaa acggcaattc atcatccatc cgggcgagtt
    13621 cgcgctcggt atgacatacg aaatcgtagg cgtgagcagc agcatggtcg gacggcttga
    13681 gggaaaatca tcgctcggcc gcatcgggct tatcatccat gccaccgcgg gctatatcga
    13741 tccgggcaat aaactcaagg taacccttga actccacaac atcgcaaacc ttcccatcaa
    13801 gctctattat aaaatgccga tcgcgcaaat atcgttcagt cccgtttcgt cgcccgtgga
    13861 aaacccttac ggcaaagaac ggctcggcag caagtattac ggcgccatga aaccgcaggc
    13921 atcgcagtat tggaaaaatt tcaaaaaaaa taacgactgg ataacattcg aaaaataacg
    13981 atatggaaaa agcgaatttt catgcctata agcccgattt cccgggagaa cagtggacag
    14041 aaggagaacg tctcatcctt tcgcccttcg caagcagtct cgacgaaccc gttgccgtcc
    14101 tgcgcaatct tccgccggaa atcgtgggag cgttgtgttc gcgcgcaagc cgcgtcagcg
    14161 gatcgctcat ccgcgcgctc cacgatgaat atttcaaacc gatccttgac ggcgaccccg
    14221 aactcgcaaa agagcttcgc gcgaccatcg agtttttccg cgtgcgcggc tttaaaaata
    14281 tcctgaacaa tcagcgcgcg caggcattct atgcgaaatg gctttcccaa tacggcgacg
    14341 attccatcgc gcaaataacc ggcacgcata tcgttttttg gggaatctcg caagtcgcca
    14401 tgaaatacat cgaagaccag cgtatcggcc tcgaacctat cgaaaaatcg acgcgctatg
    14461 tcaatttcgg cgagaagatc ggcggcagct atctttatta caccccgaag cccgatcttg
    14521 aaacggcaaa attactcgat gaataccgcg caatcatgga cggtcttttt gaatcctata
    14581 acggcctggt cgcggatctc acggcgtggc ttaaagaaaa ttatacggaa aaagatgcgg
    14641 tactcgaaaa gaaatcattt gacacgctcc gcggcattct tcccatggca acgatcgcgc
    14701 aagtcgcgtt tcgcggcaac gcgcaggcgt ttgaatacct tatcaaccgc acggcggcgc
    14761 acgaactggg agagttccgg tggatcgcca gcgcgttaaa aaccgaactc gataaagaaa
    14821 tcccctcgct ccttctgcgc gtcaacgacg agaaatccgg ccgctaccaa tcctatcttg
    14881 ccggccgccg cgcgaacacc gtaaaagccg cgcggaaaat tacacctgaa ctctttaaag
    14941 aaataacgcc ggcggcgcat cccgtcgtcc ggcttgcgga atacgatccc gaaggggaaa
    15001 acaaaatcat cgccggcatt cttttttctt caagcgcgga aagctggatt gcatgccttg
    15061 ctgccgcgcg gcgcctttcc gaaaaagaaa aacaggaaat catcgatgcc tacctgacgg
    15121 gcagggatgc ccgatggcag aaagtcggac gggcgctcga aaactcatac gtgcggttcg
    15181 atattcttat ggacagcggc gcctatcgcg acctgcaccg tcaccgcatg ctgacgcagg
    15241 aacgccagct tttttccgtc gcgcacggct atagcgtacc gcaagaaatc gtcgatgccg
    15301 gccttgaaga ccgttatcgc gcggcgcttg attccgtcgt gccgctcttc aaaaaactgg
    15361 aaactgtttc tcccgaactc gctcagtacg ccgtgccgct cgcataccgc gtccgcttct
    15421 atcaatatga aaatctccgc cagttcttct gggaagcgga actgcgcacc ggcccgcagg
    15481 gccatcccca ttaccgtttt attgaacagg aaaaataccg cttgctcaaa gaaaaatttc
    15541 cgcatcttgc aaaggccatg cttgtcgaca cgaaccaata cgatttcgcc cgccgcggcg
    15601 ccgaagaagc gataaaggat aaagaagaga ggatcaaaca gaaaattcgg gggcgttcat
    15661 aaaaatcccg ggcttaatat cccgggattt taattttttt cgctcttcgc ggacgcgcaa
    15721 acggcaaacg gctatcgctc aatattcagc gcaccgcaca ccgcatccca cactttttcg
    15781 ccgacatcat caagggaaag cagggttccg ttttccacgc attcgataac cgtaaaatct
    15841 ttcggaaaaa gttccgcaat ttcaagataa actctttctg cgcgttcaag gtgggaaatg
    15901 tcactctcgt gaaggtcgcg ttttttgccg ccgatatacg cgcggtcgcc ttttttatcc
    15961 acaaacccct gggcaattgc cgccggcaca tgaagaatga tattgagatc cggcttggga
    16021 atcccgagcc tgccatactc aagatcataa agccacctaa aaaaatcctg cctcttcttt
    16081 ttatcatcaa tcttccctcc ctgatggccc atattggaac cgacgtagcg gtttgcaagc
    16141 agcgttttcc cttcggcaag ccattgcttc atctttttct cgctcgcatc aaaacggtca
    16201 agcgcataaa aaagcgatgc tttttcgggc ccgatttctt taagcgtacc gtacttcccg
    16261 ttgagatatt ccgtgacgaa atacgacgac ggctccccat atcgcggaaa gtcgagcttt
    16321 tccacggcga acacctcctt cagtttcggc aacagccggt tgaactgttc cgtcttgccc
    16381 gaaccgtcgg ttccttcaat c
//
LOCUS       DAIDDT010000010        16122 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_10_length_16122_cov_6.043565, whole genome shotgun sequence.
ACCESSION   DAIDDT010000010 DAIDDT010000000
VERSION     DAIDDT010000010.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16122)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16122)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16122
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10_length_16122_cov_6.043565"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3880 a         4393 c         3939 g         3910 t
ORIGIN      
        1 ttattaaatc aatgaaaaag tccgtttttc tcgcattctt ttttctctgc atagtgagtt
       61 atggaggaag tgccgttgcg gcgcgcgcgg caggacagca aacgctctcg cccgcggaaa
      121 cggcggcgct gaaccggacg cttgaaaata tgaaaatgac gttgctccag atgcaggacc
      181 aacttgccgc caagcaaaat tcgaacgggc aggcatcggc aaacgggtcg ccggcacaaa
      241 ccgcaacggc ggcgacggcg gtcatctccg cgcaggatgc ggcatccctc aggaacgtgc
      301 tgactgcgct cggtgtcgtt ctttcaagtc ttcagtcaaa cgccgtgata aatgacagcg
      361 ttatgaccat gaaccagaaa accgtcatgc gttccgtatt gaacggcatc ggcgcgaacc
      421 tctcgtccat gaataatatg cttgccggca atccggcgcg cggcgtgagc gcaataacac
      481 aggcctcggg agtatcggct ccgatagttg cgcgggttcc gagccggtcc gcatccggca
      541 acgccgtttc cgttccgcag gtttccgata acgcgaaccc gggattggcg cagattgcaa
      601 caacggcgcg atggagcaaa tggagctggc ctatcggcat tgccatcgtc gtgattctcc
      661 tcgcgtgggc atggttccgc cgcgaaaaga aagcgcagcc cgtcgcttta aaaaacaacc
      721 gaagcccgaa aaaggatgcc gtaatagcgc aatcttcggc gggctcggga acgggctcgg
      781 gcgcgaaaca gaatactgtc ccgcagaatt ttccgaaaga acagcatcca tcgccgaaaa
      841 tcgaatcaaa accggccatg tcccctcttt cttccgtcgt gatgggaacg cccgcgcaat
      901 cgcaaccagt gatgccgcag aaattccagg aacaaaaaaa agcgacggga taatccggtc
      961 ttcggttgct tcggttccgt gctaaacaaa aagtccgctt cagagcggat tttttgttta
     1021 gatttttttc gcaaggccgc gacaacttta tcattgcccg gccgcggcgg ccgaacattt
     1081 ctctatgacg atgggcgcgt ggttagattc gcggttcgcg agatagccaa tgccaaagct
     1141 taatgatgca gtcaggaaaa tgatcgcgcc gaaaacaatt tcttttttat aatagacatg
     1201 ccaattctta gcccattcta ttttttcgtg aaaaaatctt attgccatga tttcgaaaaa
     1261 tcatagcaaa atataaatta aagaaagatt aaaaggtttg tcatgatccg tccattcacg
     1321 gcacgagata agctccgctt ctgtcgttcc cgcgaaggcg aggatctaca cttccaaaat
     1381 ttagaagaat aaaacaggaa atacaggccg tccgccggag ccctcctttt ccggctacaa
     1441 ccataagtta taaaggacga acgatggcaa aagaatcaaa aactaagata tttttcccac
     1501 tgctcgtaaa gcaggcgcgt ggcgcggatg tcgccgacat tatacttcgc gatctcaaga
     1561 aattttttct tcttgaaaag cggtcctacg tcgtcgccgg tcacgccgcc ggacttcgga
     1621 ctcgggatgc cgaacgcgcg cgaccaaaga tgcaaacttc ctttccggcg cgtcgcgccg
     1681 taaaaactaa gctgttccgc gagatcgata tgaagtgcat cgggattatt ctggtaaaga
     1741 taccgtccgc gcatcagatc cttcgtcggc cggatgcctt tgatcgccga tcgcgtgaaa
     1801 agaaacggca tatcgaacgc cctgccgttg aacgtgatga acacctggta gcgcatcgcc
     1861 agctcccaga atttttgcag catttcctct tccgtcatct gcttgaacga aatgccgtcc
     1921 tcttttatct ctttgttctt ttgtcccggc gcctggtaat acacaccgcc ttcgtttttg
     1981 tgataatcga gcacgccgat cgcgacgatc tcccccgtca aaggcgaaaa tccgaggccg
     2041 ctcttaaggt cttcgagcgc cactttatat tcatcctcgc tgttcgaatc tttttttatc
     2101 cagcgggtaa gattttcctt cgtatcttta tcgagcgaat cgaaatcctc gccgatggtt
     2161 tcgatatcga tgacaagctt attggatggg gtcatatgaa tatggaatta tttttttccg
     2221 cgcccaacgc ggcgttgttg acatgaaatt tattatagca taaaatggaa tgaaataaac
     2281 attaaggaac aaggaatgac tccccgcctt cgtaaggcgg cggtatgctc atttttcagc
     2341 ttatcgctgc gctctaaaga aaaatggcac atacaaaggc tataggatcg acaaaattag
     2401 gacgcgattc catcgcgaag cgtttgggcg tcaaaatgca gaacggccag aaaataatgg
     2461 tcggacagat catcatccgc cagcgcggca cgaaatatct tcccggcaac aacgtcctcc
     2521 gtgccgaaga cgatacgctc tacgccggaa aaaacggtac cgtggcattt acgacgaaaa
     2581 caaaaacgcg ttttgacggc acgcgccgcc gcgcgacgat ggtgcacgtg caataaacgg
     2641 gtttaaagat gcaataaaat accacgggtt tatttccgtg gtattttatt gcatcttatc
     2701 attatgccgt ccccgcgcgg aagcgaccgg cattagaata ctctttaaag caaaaaattg
     2761 cgcattgacg gaatacggcg ggaaaacaac gaataaaatc ttcttatttc cctgcggttt
     2821 ctttttccgc tttaatcttc aatgtcgcgg caactccctt tttgagatcg actgccacgc
     2881 tgtattcgcc gaattccttt atcggacggt caaggtcgat gtcaatcctt tctttcgtga
     2941 tgacgctatg ttcgcggagc gccttgagga tggtttcttt attgatggat ccgaacatcg
     3001 ccccggtctt gtcggtcttg agcgcgaaaa cgagcgtcat accgttcatc ttgcccgcga
     3061 tcgcctcaag atgcttccga aactcctctt ctttttcgtc gtgttcggcc ttcatcgttt
     3121 cgatgttctt caacgccacg gccgttgcct gctcggcaag cttgttcgga aaaagaaaat
     3181 tccgcgcata gccgtcgctt acttctttca cgtcaaactt ttttccgatg tttttcatgt
     3241 cctcaagaag gattattttc ataatgattg ttgtgtttgt tatgttgttt ccagcagcgc
     3301 gacggttgat cgagttttta ttgcgccgct ataacggtcc cttcttttat tttccgattt
     3361 ccgcattgag tacctgaagc gccttgtcga gctggggatc gcggttgttc tttatgtcct
     3421 cgtcggtaag cggtaccacg taatcgggca cgatgccgcc atgatcaagg atgcgcccgg
     3481 acggcatcac ccaatgcgcc accgttattt ttatggacga gccgtcggga agattttcca
     3541 gctcctgcac ggttcccttt ccgaaacttt tttcaccgac cagtttgatg ccccggtcgt
     3601 catgcaatgc ccccgccagg atctccgacg ccgaagcgga accgccgttt atcaggacca
     3661 ccatcggaat atccgcgagc gcgccgttcc ccgaagccgt gaagtcctgt tccggcaccg
     3721 cgcggccgac ctctttcacg accggcgatc ccggtttcag gaaatatccc gcaatgtcca
     3781 ccgcaacctc caagtaccct cccggatcgt tgcgcaggtc aagcgcgatg ccttttgcgt
     3841 tcgcggccgc cgccttttgg agcgcttggt aaaaaagcgc gtcggcatcc tgattgaaac
     3901 tataaagcga gatgtgcgcg atgtcccctt tcatttcaaa gtcaaccgtc ggaaccttga
     3961 tgtccgcacg cgtcatagtg atgtccttgg tcgcggtcca accggcgcgg aaaatcgtaa
     4021 gcgtcacttt tgttcccacc gcgccacgga tgagattcac cgcctgatcg acgcccatgc
     4081 cgtccgtcga ggacgcattt atcttcacga tccagtcatt cgttttcaat ccggctgctt
     4141 ccgcaggcgt gccttttaaa ggagcaatga tgacgatctg gctgctcgta ctcataccaa
     4201 gctcagcgcc gatgccgccg aaatctcccg taatatcctg ccggaactgc tgattatcgg
     4261 ccgggctgaa aaattcagag tacggatcgt tcaatgactg gatgaggccg ttgatggcgc
     4321 catgcatttt ttcttcgttg ctcacgtcgg cattattcag ataaagatcg ttcactttct
     4381 gccacgcgtc ccaaaaaacg ctgaaatccg cggccgtgga gctcgcgccg tcaaggttca
     4441 tatttgccac gccggtgacc gttatattct ttgaaacgct tttcccggaa gcaaaacccg
     4501 caaaaaaacc gccgcctatg agcgcacagc cgacgaccgt cgcggcggaa acttcaatga
     4561 cttttttcct gcttatgttt cttatgatat tttttatttt cataactctt attgtatgag
     4621 aagcggtttt aaaagtccaa ttttgcggtt tttattccat ctccgatgat ctattatatt
     4681 atatttaaaa cccatctcaa aaaacggact gtatgggcat gtcccgtcat tctttgaaag
     4741 gaaaactttg tatcacgcta tattccgcgc cggtcttgtc cgccggagtt ttagcaaaag
     4801 ctgaccgcgc aagctccgat tccataagat caagcgacgg cgcggagaaa ctgaactgca
     4861 ccggtttttc tatctgcggc agggatgccc gcgccgtttc tttggcgaat cgcgcaagcg
     4921 tgatatgtcc cgaaaaaatt ttcatctctc tttggaaaga aacgccgcgc gccgaaagcc
     4981 cgtcctcaag gacgcttttt atttttgcga tacgctccga cgacgcgcgg tcggcggcaa
     5041 gccagatcat ctttaccgtc cctggttttt cggaaaaaaa tccggcgccg cgcgcggcat
     5101 tgttgtgcgc ggaattttcc ccgcggccgc gcttcggcaa aaatggacca taacttattt
     5161 tttcgaacac aatctccggc ggctcaaaag tccgggatac cgccgccgcg gcatccgcaa
     5221 tcatgggaag catcgcatcg tccgtattcc ccaaaaaaga aaccgtgata tgccaatgct
     5281 cccgcggcac aaaccggatc cgcccgccgg aaaaatcttt gcacttcgcc tcgagctcgg
     5341 ccgatatctt ttttatggat cgcgcaaccg atgccggcaa cggcaacgct atgaataatc
     5401 tctttttttg catagtgaaa gtataacgca atgatgcctt cctttgcgag ccgccgcctt
     5461 aatgccggat atccgattat ttactcgtgc ttattttcag tttcccgttt tcggccgtta
     5521 gcgcgatcgt cccgttttcg tcggtgcgcc aaacaacggc attggtcgac gacgcgatgc
     5581 gcgcgagcgc ttcttttgcg ggatggccgt atttgttttt cgcgccgacc tcgatcacgg
     5641 cgactttcgg atccaccgct tcaagaaaag catcgcccga agaaaacttc gaaccgtgat
     5701 gcgccacttt caggacatcg gcgcggacat cgatcccctg cgccaaaaga tgatcctcga
     5761 cattgaatcc gatatcggcc gtcaaaagcg cgcggaatcc ggcggccttc acaagctcaa
     5821 caagcaccgt gtcgttcaat tccgcgctcg cgagaaaacc cggatcgggc gaaagcatgt
     5881 ctatcgcgcc gtctttataa tggatacggt cgccgccggc gagcgtgatg atgggaatgc
     5941 ccttcgaccg tattttttga aaaagcgccg gccattcgct cacgcccgcc gtatcgtcgc
     6001 ggccgttcca gatgaacgcg ccgatgcggt aatggtcgag catataataa tagccgttga
     6061 aatgatcgag ctgaggatgc gatatgacgg caagatcgat ataatcgtca cccggcggca
     6121 tcactttttc gatgctcgcg agcacgctgt catccggccc ggcatcggtc atcactttta
     6181 tatttccagg aaaaataata agctcgctgt cgccttggcc gacatcgaga aaatacgcat
     6241 gcgtattgcg gcgcgcggtt gcggtcgaaa aaatattcca ccagacgacc gcggcaagcg
     6301 cggcgaggaa aatgataatg atcgcgctga acgtggtgtt atattttttt gtcgccatga
     6361 tgataatgca gcgctataaa aaatacgagt gcgccgtaat aggcaaacac aacggccgtc
     6421 gaatcgaatt ttatcgggac cggaaccgga aggccggcga acagccgtat catcgcaagc
     6481 tcgtaaaaaa gaataaggcc ggcgagcttg gcgcaaaaaa acgcgagaga gtgcgaaata
     6541 aatccgaacg cggcaaggag gaacccgaaa aacatcgcga gcggcacggt cgaaagaatg
     6601 aggatattcg cgaagatcgc cgtcgccgaa aattgcccga aagcgatgat gagcaccggc
     6661 atcacggcga gctgcgcgga caaagtcgtg acggcgcatt ctttccattc gagaaaaccc
     6721 gcgccgttat aacggaaaag ttttttgagc ggcggaccga gatagacgat gccgagcaga
     6781 ctcaaaaacg aaagctgaaa accgacgctc gccataagca tcgcgggatc gtacgcgacc
     6841 atgaccgccg ccgcgaacgc gatcgcattc cggatatcgt agacccttcc tgtcactttc
     6901 gcgaaaagcg cgatggagcc ggtgattgcc gcgcggatcg ccgagggctg aagtccggtc
     6961 atcaaaacga aaagcagcat cagcgcgatg gaaataaaaa atgttttccg gcgcgggagg
     7021 aaacggccga acacggattc cgccgcaaga atgacaatcg tgatattata acccgacacg
     7081 gcgacgagat gcgtggtgct cgacagagcc atcgcgttct tgagctcagg actgaaattc
     7141 actttcgatc cgaacgcgat gccgcccaaa agcgcggcct gatcatccgg caggacggct
     7201 tcgaagttcc tgagcaccct tagcttgagg tcgataagct tttcgcgaag ccagaatccg
     7261 ttatggcgcg atattatttt tattttcggc gagaacacga cgggatcgcc tcccggcgca
     7321 tccggcgcgg cgatgatccc gccgatctga agcatgtcgc cgtacgcata atcgccggtc
     7381 ggcggcgcaa aaatctttac cgtaccggcg aaaggcggct gcacatccgc ggtaagaata
     7441 agaaatttct gcgacggctg cggctcatcc gaaacaaccg ctgaaaaaga aatataacgg
     7501 ttataaacaa tgtttatttt cgccgccgcg gcatcggcgc gccaatgata atagaatgcg
     7561 ccgaagagca tgagcacccc gaagaacaaa aattccttcc aaagggaaat ttttttgatt
     7621 aaaaccagca atccgaaagc gcacgcaacc gccgcgccga aaattatcgg aatattccat
     7681 cccatcgccg ccgcaaaaac gccggataaa aatcccgccg ccgcgacgaa cgccctatcc
     7741 cctatcccca atgccattcc gacatcctat aaaaaatgag gtgaaaaaaa gataaaggat
     7801 tccgcatttt tgctttcgtc cgaaagaggg gctcccttga ctccttcgca agcaggaata
     7861 ctccgtccgg cgcggaaaaa tccgggtact gaaaaagggc aacgcctcgc gcactgcccc
     7921 tttttgatat attccgaatt ccgtatccta ttactttact gcttgctgcc aggaattccg
     7981 ccggtggcgg ccctgatctg gatcgattgc gcggtcaaac tgccgtcggc gttctgcgtg
     8041 ccgccgatca tcacgtgcgt tccaggaacg acatcgctca acgatgccgc ggtcggcttc
     8101 acgacctgcg tcgaacttga gtaaagcacc acttcagaat ttccgttcgg gagctggagc
     8161 gtgatgctct ggctatcctt cgccgtcacc tggccgctcg caaatccgcc gccattgccg
     8221 ccgcgtccgg caccgccgcc atacgctctc gccgatgaag aagaaaattc ttttcccgcg
     8281 gaggtcgtgc ccgccgttcc ttttccgtaa aacatgcctg cgaaaaagaa aacgatcgcg
     8341 cacgcaacgc acaaacctat cgccaaccag ttctttttca tttttttcat tttgaaattt
     8401 attatttaaa acgacctttt atattttttt cgttattgcc gatttaacgt ctctattatt
     8461 atctgtaaat acggacgccc gtgctttaag tccgtctcaa aacattggtt tccgttttcg
     8521 cggaaacgac gaaaagaagt tttcaacata tcctcttccc tgattgccaa caactaattc
     8581 cccgtcacta ttcgtaccgc aacgcttcaa tcggattcag tcccgccgcg cggcgcgccg
     8641 gataatagcc aaaaataatg ccgatggccg ccgaaacgca gaatgcgaga atgatggaac
     8701 caaaagaaac gctcgttgcc attttcgcga aataggaaat gaaaaatgcg ataagccagc
     8761 ccaaaataat gccgatggcg ccgccggtga acgtaagcat aaccgactcg cccaaaaatt
     8821 gtttcgtgat atctttcttt tttgcgccga ttgccttgcg taatccgatc tcgcgcgtgc
     8881 gctccgtaac agtcgtaagc atcatattca taataccgat accgccgacc agaagcgaaa
     8941 tacccgcaat ggcggcaaga agcatggtaa aagtgcccgt cacgcttgat gccgcggcga
     9001 cgatatccgc ctggttcaaa acgctgaaat cagccagtgt cggatcggaa atattgtgcc
     9061 gcgaaagaag aaggctcgtg atatcgttct gcacctgcgt catcgccgcc tcgctctgcg
     9121 cttccacatc aacttcggta agatacgaaa cgctccccag aagaaaatgc tgcgcggcgg
     9181 aaagcggcac atagatcata tcgtcctgat tcgagaaacc gctgccgccc ttcgattggg
     9241 tcacgccgac aaccgtaaaa tcaaggtttt taatgcggat cgtctgaccg atcgccatat
     9301 ccggcgtcgc ggtcgcggcg gcgcttccga aaagatcctg cagaaccgta ggaccgatga
     9361 cggcaatacg attcgaggta ttcacgtcgg tatcggttac gaaaacgccc tgcgccatct
     9421 gcaaattgcg tacctggaga tatgccggca cgacgccgag gacggaaacg ttggtattcg
     9481 cacctttata aataacctga taacgcccgc ttacttcggg cgaaaccgct tcgacgttcg
     9541 cgacgcttgc gatcgccgcc gcatcgttgg gcgtgagcgt ctccgagctc ccccgtcccg
     9601 cactcacggt cgaaccgaag gtgcggctcg cacccggcgt aataataata agattcgatc
     9661 cgatggactg gatgctcgac tggatggttc cctgcgcacc ctggccgatg gcgaccatgg
     9721 cgatcaccga tgcgatgccg atcacaatgc caagcatcgt gagtcccgac cgcaccttat
     9781 tcacggtcac ggcgccataa gtttcttcaa taagatcttt ggtagtcatg gtagtatgta
     9841 ttttcctccc ctctgtcgtc cccgcaaagg cgggaaccta tgcttccggt cttaattttt
     9901 ttaagcatga atcctcgcct ccgcgagggc gacacaataa gcattgtgcc gcttttcgct
     9961 cttcactttt agttctcatt gatttcattg tccgcgattc tttttttatt atttttttcg
    10021 tcgcggacaa tattcccgtc aagaatgtgg atgatgcgat cggcgtgctc ggcgacgtac
    10081 aattcgtgcg tgataagcac gattgttttc ccgtggctct tattaagcga ctggaacgta
    10141 tgcagcacga ctgcaccggt ctttgaatca agattgcccg tcggttcgtc ggcaaggata
    10201 agcgcagggt cgttcacgag cgaacgggca atggcgaccc tttgcatcat gccgccggag
    10261 agttgattcg aatggaggcc ccagaatttt tccggaaaag cggaatcgtt caacgctttt
    10321 ttcgctatcg cttcgcgctc ttctttccct atgtcggcat aaatcaaagg aagggcgaca
    10381 ttccgaagaa ccgtcgtccg cggcaaaagg ttgaacgact ggaacacgaa tcctattttt
    10441 ttcatcctga tatcggcgag ctcgtcatcg gaatgcttgg cgatatcact gccgtcaaaa
    10501 atatactcgc cgctggtcgg cgtatcaagc gcgccaagaa tatgcattaa agtcgatttt
    10561 ccagatccgg aaggtcccat gatcgccaca aactcgcccg cctgaatggc aaacgacacc
    10621 ccctttaaaa cgacggtttc catggcgccg ttcgcgtata ctttttttat atctttgcat
    10681 tcgatgatat ttcccatatg ctgtaatgtt gtaatgtaaa tacgcgcatt catgcattga
    10741 aacactataa cttcttatcg ttccgccgct catccgccga atctgccgcc gcccaagatt
    10801 ctcccgaacg aacccgcgct tgaaccgccc gccgccgccc ctgtcgtgcc ggcggaagcg
    10861 cttccggaag taacggtctg cacgataaac ggatcgcccg cctgcaagcc ggaagtgact
    10921 tcggtcatcg tatcatttga aagccctgtt gttaccgctc tatttttcgg cggaacggaa
    10981 agcacgatgc cgccgttcgc gcttgcggca atgtcgctgt ccgaaaccgt tcctgcgggc
    11041 atctcgatat aactcgtcga accctgtgtc tttatggcag agttcggaac ggcaatgacg
    11101 tcggcgtgaa cctgggtgat aatcgccgca gtaacgctca tgcccggctt gatctggtcc
    11161 gtcgtatcgt taaagcttat ctgaatattg taatttacga cgccctggct taccgtcccg
    11221 accggatcca tctcgatgac tttcccggca agcgaaagcc cgcttatggc atcgaatgtg
    11281 agcgttgctg catcgccgac agcgaccttt gccgcatcga cttcattcaa agtaatcacg
    11341 gcaacctggc cttttcccac catgctcact gcgggagaag gaacagtttc accggcaacg
    11401 gcatccaccg ccgagattac gccatcaatc ggcgcccgga tatagcaatc gtcaagctgc
    11461 tgctgcgcat tctgcaccga aatctgcgcc tgctctatgc tcagctgctg ggattgaata
    11521 tcaagcggat caacgccggc aacaagttgc gccaaagtat cgctcgcttc gttaagcgcc
    11581 aacgtatcgt cattgatggc ggttttgtcc gccgcaacgg tatttatcgc gcctgcaata
    11641 tttgcattgt cattattgat ggtgttcgta tcattcgcga aattcgtctg gaaggtattc
    11701 gttatcgcag gaagctgaga aagaccgagg ttcgtcggat agttattggc gacgtaattg
    11761 agcatatcct tcgccgattt cacggttccg cttatgagct tcgccgtcgc gtacgtttcc
    11821 gaaaaaatat tgtcgagcgc cgcttcatca gcattgcggc tggtagtatg ataatcggca
    11881 agattttcgc tgtaagccgc tgcggcggcg gcataatgcg aaaccacgtc atcacggtag
    11941 ggaagcgcgc ccgcctggag gtaattcggc atcaagttta cataagcatc tggattgctc
    12001 tgcaatttat ccacctcggt cccctccacg aaatcctgca attccgtcat cacggtctgg
    12061 aagtcgacga aagcggaccc gagcgtatcg aatccgttct ggtaatcttt cgcaagggtc
    12121 gttgacgcgt tcaaaagatc ctcatcggct ttcgtcacgg catcctgatc ctgcgccaaa
    12181 gaaaccgccg taggcgcttc ctgaagtttc gcaagcgcaa gctgggctga ttgaagggac
    12241 agctgggcct gccgtagcgc cttttcttcg ttcgtcgtat caagcgtgac aagcagctga
    12301 cccgccgtaa catggtcgcc gacgctcacg gaaacttttg ttacggcctc cgtcacttgc
    12361 ggcttgatat cgagttccgt caccgcctgc gtctgcccgc ttcccgaaac cgacgcggtg
    12421 atcgtaccct gcgtcgcttt ttcgataaca tatttcgtga ccgtaatccc gctctgcgca
    12481 gtgttatacc aataataacc gcctcctacg aggataataa ctgcgagcga ggagattatt
    12541 ttatggaccg aaattatttt atatatttta gcgaacataa ttgaagaatt tttatttttt
    12601 tacggacatt atggcgtcgg ccccggcggc aacggtatgg atgacgaaac gccgaatact
    12661 cttataaacc gggcgcgtat ctggccatcc gcattcggct cgccgatcac cgcaaccgtg
    12721 tcgcctatat ttatcatggc ggccgaaatc gtgtcgttca tcttccggat aaccgtatcg
    12781 gacccgatcg ccacggagcg ttcattgttg tcgccgtccc ggatagcgat cgtagtgctt
    12841 gaaacgccga taaccgaccc cgcgacgccg tgcatattcg gcgcatattc atcgaatccc
    12901 tgcaacggcc ccctgcggac attgccgtaa aaattacggt aatagttccg ccccagcgaa
    12961 gaagaaaaaa gcgccttgcg gtatcccacc gcgacgccaa ggccgaagat caaaaatgcc
    13021 acggcaatgg cgagcatgac caaaagcagc cttttgaatt tttgcgacga taaaaattct
    13081 ttcattattt acggaaaggc gtgcatggac ctgttgagaa cctgtctcaa aaatagcttt
    13141 tcaagaagaa tccgcaagac ccgctggccc attttcgaga cagtttctat tttgaagcgg
    13201 accattgata cgcgcgatat gagcgggtat tcagggacca ttgcgatatc gaccaaagga
    13261 aaaagacaat ccctccaaaa aacaaagcaa tgggaatcgc cggaatcgac tctgcgacgg
    13321 aaagaaagaa atcgggaaaa ttcgcgagcg ccgaagaaaa atcggaaaaa ataagcgcgg
    13381 cgaacgagaa aaacccggac cgggaaagct ctgtggcggc gttctcaaac ccaaaaccgg
    13441 taagcgtaac ggatcccgcg acgcacgccc cgaaaccgac cttctttgcc aaaaacaaac
    13501 gccgttcata ccgttctatc cggcttatta tcttttgcgc caatcccggc gatagttccg
    13561 gcgcattatc aagaacggaa gcgtgttttt taggattact tttcatcgcc ttgactatag
    13621 tacgatgaga tacataaatt tggtgcatga ggaatagaat aatagaatat agaaaataga
    13681 atttcaccta ctatatgcct atatgctaaa tgctatattc tgccgtaacc tctcacaccc
    13741 ctcttttccg tcgccatcga ccctcgacca ggttgagggc aggctccggt cgaagatccg
    13801 cactttgaaa tattcggaag aagcatggat cccctccttc ggggggggac gacggagggg
    13861 aaaatgtttc gacggaatcg gcactggtta tagacagtga gaggttaatt ccactttatt
    13921 gactttccgt aaaaatcgcc ctataatctt cattatgaaa aaagaaaccc atcctgtgta
    13981 ttttaccgac gcgaaagtca tttgttcctg cggcaacgcc tttacgaccg gcgcaacaaa
    14041 gcccgaaatc cgggttgaaa tctgcggcaa gtgccatcct gtttacacgg gcgagataaa
    14101 actcatcgat accgccggtc gcgtggaaaa attcaaaacg cgccgcacga aagccataac
    14161 cgccccgaaa gaaaagaaag taagaaaaaa gaattaacaa ataaaaatcc ctgcaaaagc
    14221 aggggttttt attcggttgt attgttctga tgttggattt tctacactat aacgctctct
    14281 tcattacgac gtcatctcgg cgttcgtaat cgttcacgcg cctcagctgc gaggcgcggg
    14341 cagcaatctt tcgaaggcgt actaaatgta cgacggagaa agattgcggg cacccgcaac
    14401 aaagcagatg aggatacgtg gacgattaca attattaaac catttttatt tcaatatcca
    14461 cgccggcggg aagattcagg ttggtaagcg attcgatgat cttctggttc ggactctgga
    14521 tctccacaag acgcttatgg acgcggagct caaactgttc gcgggcgtcc tttttgacga
    14581 aggtggagcg gttgacggta tagcgtttca cttcggtcgg caacgggatc ggccccacca
    14641 ctttcgcgtc gttcctttcg gcggcttcga tgatctgccg cgcggaattg tcgatcaatt
    14701 tatgatcgta cgatttgatg cgcagtctca gtttttgcgg aagggccgat tcctgttttt
    14761 ttgccatgaa tttttagctg ttgtaattgc tgcagtattt tagcgttgta atgcggtgtc
    14821 cgtaattgaa tttgattttc aatataacat ttgaaccatt acaacgttcc cgctcttact
    14881 taatgatctt cgtcacgacg ccggcgccga cggtcttgcc gccttcgcgg attgcaaaac
    14941 gctgtttgtc ctcaagcgca acgggcgaaa tgagcttcac gactaatttt acggtgtcgc
    15001 ccggcatcac catttccatg ccttccggaa gcgtcacttc gccggtcacg tccgtagtcc
    15061 tgatatagaa ctgcggcttg tatcctttga agaacgggga atgcctgccg ccttcctcct
    15121 tggaaagaat gtaaacctcg ccttcaaatt cggtgtgcgg cgttacgctg ccgggcttca
    15181 cgatgacctg tccacgctcc acgtcttctt tcttgatgcc gcggagcagg acgccgatgt
    15241 tatcgcccgc cataccttcg tccaaaagct tattgaacat ttcaatgccg gtcacgacgg
    15301 tcttcaccgt cggtttcaaa ccgacgatct cgacttcttc gttcactttc actttgccgc
    15361 gctcggctct gccggtaacg accgtgccgc gtccttcaat cgaaaaaacg tcttcgatcg
    15421 gcataaggaa cggcttgtcg agttcgcgga ccgggtcggg gataaacgta tcgacagcgg
    15481 caatcagatc caaaatcggt ttcgcatcgg gatcttccgg cgatttcgta ttcaacgctt
    15541 tcagagccga accgcggatg acgggcagcg tatcgcccgg ataatcctgc ttcgtcaaaa
    15601 gttcgcggac ctctgactca acgagatcga tcaattcagg atcgtccacc atgtcgcatt
    15661 tattgaggaa cacgacaatt gaaggaacgc ctacctggcg ggcaagcaga atgtgctcgc
    15721 gcgtctgcgg catcggaccg tcggtcgcgg cgacgacgag aatcgcgccg tccatctgcg
    15781 cggcgcccgt aatcatgttc ttgatgtaat cggcgtgccc cggcgcatcc acgtgcgcat
    15841 agtggcgctt ctcggactcg tactcggaat ggtgcaatgc aatcgtgata ccgcgcgctt
    15901 tttcttccgg agcattatcg atttgattga cgccctcttc ttttttcgca agacctttca
    15961 tgaacaaaac gtgcgtgatc gcggcggtca aagtcgtctt gccgtgatcg acgtgcccga
    16021 tggtaccgat gttaagatgc ggtttcgccc gggagaattt ttctttttct gccatggttt
    16081 tattttaatt aaatttattt aaatttttta tttattctca ct
//
LOCUS       DAIDDT010000011        16010 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_11_length_16010_cov_6.343062, whole genome shotgun sequence.
ACCESSION   DAIDDT010000011 DAIDDT010000000
VERSION     DAIDDT010000011.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16010)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16010)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16010
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11_length_16010_cov_6.343062"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3715 a         3837 c         4275 g         4183 t
ORIGIN      
        1 gcggtttaag atattttctt atattttttt caaatgcgta ggtcctattt tttttaccgt
       61 tttttgctga atgggttgat ggtaccgtac acacgattgt ttttcaagtt tttcggaatt
      121 ctttttttgc gttcttcgcg tttaaggagc cgcgcgacgg cggcggaatg tgctataata
      181 gttgtatgga accgaaggat ccgaagcaat tcgggaatca ttttctcgtc gcgctcttcc
      241 tcgcgtcggc cgcgcttgtc gtttatattt tcctgccata cctttcggtg gtcgttttcg
      301 caatcgtctt tgccgtcgtt ttcaatccgt tgtatcgcgg tcttcgtaaa ctggtccgtt
      361 atgactggct tgcggcgctt atcacggtgc ttaccgttat ccttatcgtg ctggtgccgt
      421 tcagttttct tgcgacgctc atttttgggc aggcgatgca tctctacgga tggtttgcgg
      481 gcggcaatga agtgcatctt ggcgcgatcg tgaacggttt tctcgcgcgg catttcagcg
      541 gttttgattt cccgcagata gacatcacga attatctcag catggcgctg ggctgggttg
      601 tgcagaatct gctgccgttc gtcggcggtc ttgcgcagtt cgcgctcgtg ttattcctga
      661 gcctgttcgg aatgttttat cttcttacgg acggcaatgc gcttcgcgat aaaatcgcat
      721 cgcttattcc gcttgagcag cgctattcaa aacgcgtgat cgaccggatg gagcgggcga
      781 tctattcggt ggttggcgga tcgctcgcga tcgccctgat tcatggcgtc gaggccatcg
      841 ccggtttttg gatattcggc atcccgaacg cggcgttctg gggatcgctt gccgttgttg
      901 ccgcgctcat cccgatggtg ggaacttcaa tcgtggatat tcccgccatc gtctatcttt
      961 ttgcgaccgg ccatatcatc gccggcatcg ggttgttcgt ctggtggatc gtcgtttcca
     1021 tgaacggcgt cgataatctt ctttcgccgc aactcaagcg ccgcggcctg aacgcccatc
     1081 cgtttctgat cctgctttcc gtactgggcg gcatcagcgt gtttggcctt atcggattcc
     1141 ttatcggccc gctggcgctg gcatttttct ttgcgctgtt ggatatttat ccgttgcttt
     1201 ccggccgtga accggaaccg catgcatcag gatgattttg acggcggttt ttgatgctgt
     1261 tattaaaaaa ataaaaaacg acgcttcatt gtgaagtgtc gttatgtttt ggaaagagaa
     1321 ttataattct ctttcatcta aacctctcat ggtaaacacc tcctttttaa atttttttaa
     1381 aaatcattaa ataggagaat ttcccagccg cacgcctaag tgcgaggcgt ttttacattg
     1441 taacacggac ttcttttccc ttaaaatgga tttttcggcg gtcttatccg cttctgtttc
     1501 atttttctca cctccttatt aagttgttaa agaactggcc ggaatccgtc taaaaaagac
     1561 gaaattttcc agaattcccg cacccaaatt ctatttatag aatagcatat attgattcga
     1621 tgcgccagtt catttcattg ttctcgacct gggcgaggat tcacgcttta agaaacagcg
     1681 atgatgttca ttaacattat tctctttctg tcatttcgga aaacgcgcgt tttcgagtgt
     1741 ttatctggaa tccaggatta atttgaattg aataatgaag agggttcatc ctggatctcg
     1801 gatatttttc gcaaagcctc aaaattccga gatgacagac ccgaaaggaa aaacaatgag
     1861 cgattaagaa aaagatggaa aaaaagggag gcagtgtgag gggacgacgg agggaatgca
     1921 atgcttaatt ataagtaatg agttatctct tctttttcta actggcgaat gccgcgagaa
     1981 cgtcgggatc gtcaaggtag gggagatgca tatccataaa aaacgaaacc atgacgattt
     2041 caccgttttt gtttttaagc gcgtcgaaat tcccgtggcg ttcccaatga tcttttaaaa
     2101 agcgatacgt tcctgcttcg agcagcatcg aggtcggtcc cagtgaatgg taatattgct
     2161 ggtagaattc ggcgctcatg acgtaggcct cccgcttcgt gatgccggtc ataagcggaa
     2221 agtcgacgat gtcctcgtat tggaacgatc cgggaaattt tttcagttcg gcgagtcctt
     2281 ccggcgaccg gcttccgtgc actttttcat tgtttgacat cgatgccacg cccagtattt
     2341 tcatctgcgg gaatccgctt ttcaaataac gttttgccgc catgatagtc gaacaggtgc
     2401 cggtcgtcgc ggcgaagatc gacggcgttt cgccgagccg cgccagttcg cgggcaatgt
     2461 gatccatcgg ccacttatgg ccgagaacgc ttccttcgtg aatatattgg ttgatgaaat
     2521 gatattgttc cggatatttt tcggcgagcg cctgcgcgtg ttcgatggat gagacgccgt
     2581 cgggcgcata gatgatttcc gcgccggcag cgatgagctg attttgcttt ccttttggaa
     2641 ccgttctttc gatcacggcg gaaaatcctt tgacgtcaaa catcggcgca ataagacctg
     2701 atgccattcc ccaattgccc gatgtcgcgc cgatgaccat aatgtccttg ttgaattttc
     2761 ccgctttttc gtccataagc cacgccatga acgcgggcgc gcttttgccg ttatgcgccc
     2821 gcatctcggc tccgaaaaac gcatattttt tgagtatttt ttcaataccc gattcgcgga
     2881 accacggttc ctctttcgtg ctgtcgggcg tcaggatcgt cctgagcgga ggcaggcggt
     2941 gctccattat atcaagcagc cgcgcgcgca tttcgtcttt ttgcgattgc gtgagttttc
     3001 tcattttttg aacgtatttt taaaagtttt ttacgaaaaa ttttcgtgat gcatttactt
     3061 tatttcatca taccatccga tttctgtgtt gcaagcagtt gtactatccc gccgtggcac
     3121 gcgtgttatt tttcctgtat aataatcacc attatgatgg aatcaaatct gccggatacg
     3181 aagacgccgc cgggcatcca gccgggacgg cttattacgg ttgacggcgt cacgtacgaa
     3241 cgtttcccga ttaaagtgcc gcgcctcatc gcattcggcg aagatttgga aaaaatattg
     3301 acggaattcg tgaagccgca tttcaagtcc ggcgactggg tggcgctttc cgaaaagctc
     3361 atttccattt cgcagaaccg cgtgcggcat attgcgacgg tgaaagtgac atggctcgcg
     3421 cggctcgtgc aaaaaggagt tatgaagcat aaaaatatga cggcgtggga caatccagag
     3481 aaaatccagg tcgccatcga ggaagcgggc cggctgcgga ttattttggc gatgatcatc
     3541 ggcggcattg gcaagcttgt cggcatacgg ggaattttct ggattgttgc cggccatcgc
     3601 gtgtcggaga tcgacggttt tattcccgaa gatatgtatc cctacacgga atgggccgtg
     3661 ctgccgcccg ccgatcccca gcgcgactgc gaagaggtgg aaaagaaact cggcatgccg
     3721 gccgttaccg cggacgcgaa tttcattaac gtgaaagtgc tcggcgtgag ccgcggcgtg
     3781 gggctcgaca agaaaacggt gcgcctcgtc ctccttgata atccgctcgg gcagggaaat
     3841 aaaatgacgc cgttcgtttt ggtgcggcgg atcgatgaac aaaaaataac cgaaaaataa
     3901 caacattatg accatccgaa gccgcatcaa acggataacg ccgtggccgc tgcattattt
     3961 gtacagaaaa atttattacc tgccgcagga tgttcccgcc gcgctcggat tcctgtttca
     4021 taaaacgaaa tcgtccacca cgttcggcga gcggctttcg cttatcaaaa atttttatgc
     4081 catttcctat tacgtcgact gtccgcacac cgaaaatgaa atgcttgcca tcgcgcgctc
     4141 tatcttgaac ctcggggcgg acgttccggg cgccatcgtc gaagccggcg cgttccacgg
     4201 cggcagcacc gcgaaattca gccttgtcgc ccgtctcgcg aatcggccgt tccacgtttt
     4261 cgattcgttc gaaggcatgc ctgaaaatac cgagacgcac ggcaaaagca tttatggccg
     4321 cgaacatcat tttccgaaag gcagccatgc ggtcggactt gaagaagtgc gcgggaatgt
     4381 ccgaaaattc ggcgacgtga gccgtacgga attccataag ggattttttt cggacaccat
     4441 gcccggtttt cgcgatgaag tcgcggctgc ctgcgtgaat gtcgatcttg tgcaatcgac
     4501 cgaagattgt ctgcgcgcgc tctatccgct tacttcatcc ggcggcgtga tattttcgca
     4561 ggatgcgcat tttccgtgga tcatcgaact tttaaacgat gatgcttttt gggaaaaaga
     4621 gatcggcatc aaaaaaccgg cgcttgaagg attgggatcg tcgaagttcg tcgccattcc
     4681 gcatccgtaa ctttcttctg tcgtcctcgc attcgtgagg gccgcagggg gagcaatgcg
     4741 aattttgttt ttaccgatga gccttgatgg gattttcggc ggacattatt tattgattac
     4801 gactttcgcg atgccgaaaa tattcctcag cgaatagtgg gcggaaaaac tgatgtcgtt
     4861 gccgttgaga atgtagggat cgcgcatgag gtaattgccc ctccttccgc ttacaaggac
     4921 gacatagtgc gtgccgccgt aagccctgag gccgacgatc accggattgc cgcttgcaag
     4981 cgttgcatcg atggttgccg ctttgcgcgt ggcggttacg ccgtcgacat taatggtgat
     5041 taaaaggtat gccggataat atgccgcgaa attgcccggg tttgcgttga tggtcgcggg
     5101 cgtcacgtcg cgatagccgt aatgcgtcat caccatcgcc atggatgtga cgagacagcc
     5161 gtcgcttgcg agattgtagc gcgttccgtt cagcgcttcg ttgccccata gcgcgtcgcg
     5221 ctggttataa tagcagcccc atgcgtcgca gttggtctgg ttcgcgagaa gttttgaacc
     5281 gccggcattc tcagcgaatg tggagaaact tttaagttcc gcttccgcgg cggcgagcag
     5341 tttttcgtaa accgattctt ttgcgtttgt ctcggtcagc agctggcctt tggattgcac
     5401 cgtggcggcg agcgattgct gctgcgccgc aagcgactgt ttttgcgccg caagcgtttg
     5461 ttgtttctgt tgcgacgccg cgcgcgaatc ggcaagattg ctttcctgcg tctgcagtgc
     5521 ctgcatatca ttttggacgc cgtgctgcac ttgcaaggtt gcgttgatgc tttcccatac
     5581 ttggacgatg gtgcccgacg agagcagctg cattaagagc ggctgatcat ttgccttttg
     5641 aaggctacgg aggttttcgc cgagcgccac ctgatcggtc gcgaccgtct gctgcgtgtc
     5701 cgtgatcgct ccgcccagct gttgaatctg cagctgggtg gcattgatct cgtgctgtgt
     5761 aacggcgacc tgcgcttcca ctttattgcg ctgcagatcg agtgtattga tggcggcctt
     5821 gagggttttt ttatccgcgc cgacttgctg aagctctgcc tgatacgtcg cgatctgctg
     5881 gttgaggctg gtgatttgct gattgttcgc gtcaatctgc tgctgcagcg agctactgct
     5941 cgttgccgtc gtcgtcgtgg cagttgcgtg ggcgacgggg gagctccgta cagttgcggc
     6001 ggcaacgaat acggcaacga tgagcgccgc gcggagcgcg aaaaacccga taatatggcg
     6061 tatgtcgttt ttgatcatga gaaaatatta tcctaaccgg gaatagcttc tatttcaagt
     6121 acgggttctc ggcaggggat tttatccgaa acatatttat aggccgcttc ccgtattgag
     6181 cgccgcgcgt tttcttatat accggtattc atggatgata agcccgatca ccgcaatatc
     6241 gacgatcgta acgatccaga gaaacaacga ggaaccgcgg ctcaattggt aagattggta
     6301 aagcacgaaa cctccgaaaa ttataatgga ggcgggatat gcccaaagct tgttgcgcaa
     6361 caatgcggcg acgagaaata ttttcacggc gccgtgcacg ataaggatga cgcccgcgaa
     6421 ggtccgggtc tggatgacgc tttgcgcggt atgcaaaaaa agattagcca ggaaatcttt
     6481 cgggtcttcg cttaattctc ctcctatgaa aaagaggatt atggcattaa gggcggcggg
     6541 tgaaacaata aggagcgcga taccgcccat cagttccaga gtgccgtcaa ttcctttcag
     6601 aaggatgccg agatcgaata gttcgcgcca tgttttttct ttcattggat gatggttttt
     6661 gaccggttcc ccgccgcgaa ataatatatt attccggcga tgaggacgaa gatgacgctt
     6721 cccgcaatat ccgcaacatg atggacgccg ctgtacactc tcgcgccgcc gatcgcaaag
     6781 gcaagcaccc agaggatcgc gctcagtttc ctgctgtaaa accagacgac catcgccacg
     6841 gctcccgtga gcagcacatg atccgacggg aaaccgttat ccgccgcgtg ggcgatgagc
     6901 gggatgaagt gtccgacaac gaaggggcgc gggtcgtaat acagaagccc gccgatcctt
     6961 gaaaggacat acgcaagcgg cgcgatgata acgccgcaga cggccatact cttctggata
     7021 ttcctcggtt ggataaaaaa gaagactgcg ccgatcaaaa gcacgaaaag ataaagatat
     7081 tgggcgatga aaattacgat ggcgttcatg attatattat agcgcattat agcgccgacg
     7141 tatactcggc ggccagattt tatatcccag tgatggaata atatccattt tatatggttt
     7201 attgcgcctc acgcatagaa aaaggaaaat atttgatgac ggacttagcg agataaacct
     7261 ccttccaact gaagcaaaga acataattgt cttttataga cggagaactc cgaacttcta
     7321 tatttacttt taaagccgcg cacatccgat cagggagacg gaaaagaagt agggtatttc
     7381 tactttggaa cctttcgctt gagaacaaat tagtagcgca aatacagtat aaaacgccgt
     7441 ttgatgtgat cgcaaaagca cctaaaaacg gcgatttgaa cacaatgctg ggggactagg
     7501 agtcggacct agattcacgg cttcaaaggc cgctgtccta ccattagacg atcccccaag
     7561 gatgttttgt gatcgctcta tgttataata tataggagaa aaaggatagt gaaaagatca
     7621 taaaaactgc acattaatca ttcattttcg gaaaaggggg attttttcag ctcctttgta
     7681 aatgatagat gttccatttg cggcagaggt gaataaacat aataataaaa atatgaaatt
     7741 cgagttgccg cagctgcagt acggatatga tgcattagaa ccgtatcttg acgcggggac
     7801 gatggagatc cattatggca agcaccatca ggcgtacgtg aacaacctga acgccgcatt
     7861 ggaaaaatat ccgcaactgc cggatatgcc gatcgagaaa ttattgcagt cgctggacgc
     7921 gctgccggaa gatatccaga cggcggtcag aaaccacggc ggcgggcatt ttaatcattc
     7981 ttttttctgg acgtgcatgc gatcgccaaa agacggcggc ggaggggagc cgcagggaaa
     8041 aatcgccgat gccatcaatg cctcgttcgg cgatttcgtg aaattcaagg atatgttttc
     8101 gaaagccgct gccggcgttt tcggttccgg ctgggcatgg gttatcaatg ataacggaaa
     8161 actttcaatc gttacgacgc cgaaccagga aagtccgatc tcgaaaaatc aaaagcctat
     8221 tttagtaatt gatgtctggg agcatgccta ctatctgaat tatcagaacc gccggccgga
     8281 tttcatcgct gcatggtggc atgtcgttga ttggaccgcg gcggaaaaaa atctatgaaa
     8341 atagcagatt aaaagattat taaattagat ttgactttgc gttttatagg gattagcctt
     8401 gggcaaggtg atgatccgcg taacgacggg tcggaaattc cgcttgtcat acaaaacgac
     8461 cgctttagtg gtcgttttat gttttagaga attttttgct gtcattgtcg gcagtgacgg
     8521 acgatgacat atcccagttt tttttcaaag catggatcct cgcctccgcg aggatgacag
     8581 aaaatactgt tttaaggttt tctaacggtt tcgatttgtt ttcgcctttt cgccttcgcg
     8641 ctgggttgtg gtgagatgca atgcgccgtt ttcaatttcc gcctcaagca attttacccg
     8701 tttgccgttt tgcatggtcc agacgcccgg ttcgggattg agggcgcgga tcttccggaa
     8761 tagcacatcg gttttttgag catcgccgtt ctcggcatcc tttaaatctt ccggcgtgac
     8821 gaatccgtct tgagtttgga attttttcgt gactatcgcc gtgttttcat cttgcgggat
     8881 acttttcatt tcgccgttta tgaatttcgg catcatttca acgagcaatt ttccgccgag
     8941 ttttgccagc tctttttcaa gctgcagata agtttctccg gaagcgagcg gtatgcggcg
     9001 cgcggctaaa atttccccat ggtcggtttt ttcatccatc cggtaaatgg taacgccggt
     9061 ttctttttcg ccgccgagga tcgccgactg gatgggggat gcgccgcggt atttcggcag
     9121 gagcgacgga tggacgccga gtgttcccat ttttgggata tcaagcgccg ctttcggcaa
     9181 tatttttgcg tatgccgcga cgacaaaaaa atcgggatgc aggccacgaa gctggttcat
     9241 gaaatcatcg ccgatttttt ccggctgaag gatctcgatc ttgcttttcg cgccgctgcg
     9301 gtttgcgtcc tgtattcttt ttttcgtttc cggcgccgtc acgatttttt tccgtccgac
     9361 ggggcggtcg ggattgcaga cgagcgcaac gggcggcata tccgcgcgga taagagcgtc
     9421 caaaacaatt tccgcgaacc gcggtgttcc aaaaaaaatg tagttcatag tgcgatgcgt
     9481 tttatagtgt cggagtgtct ttaatagtgc gatgaatttt cattacaacg ctataacatt
     9541 acaaaacatt acaacattta agcattattt tttcggcgcc gcttcatgca ctttcttcgt
     9601 tcggtctatg aaaagtttcc cttcaagatg gtcgatctcg tgctggaaca cgcgcgcaag
     9661 gaatccccac gcttttattt ttaccgcttt gccgttccta tcctggccgc tcacgattat
     9721 tttctccggc cgttccacgt cgccccattt tccggggacg ctcagacatc cttcttcgaa
     9781 atcgatcgtt gcaccgcccg tcttttcgat tttcggattg aataccgcat agaatttcgt
     9841 tccgccttgc ggatccggca cttcggcgac gaacatccgt aggtcaagtc cgatctggtt
     9901 cgcggaaagg ccgacgccgt tcgctgcgcg cataatccgc ttcattttcg cgaggagcgc
     9961 cgtgatttcg cttttcttcc attttttgaa atcaaaatcc gccggttttt tccttagcac
    10021 cctcccttcg tctttgtttt caatgagaag aattttatta ttcattcttt ttgacgatcc
    10081 gttttttcgg cgcgggtccc tggcgcgcga ggcagtacat aaaatatgcc cctttatttt
    10141 tcacatcctt ccgttccgcg acgttttttg cgagctgcag cagttttcct tcgtcttttt
    10201 cctttgcaag ctttatatag agcgatgtat ggcttttatc tcccaggatt tcggcgacca
    10261 tgaggccggt catctgatat ttccgataga tatgggattc tttcgcccgc tgccgcaatg
    10321 tttttatata tttttcggac ttggtgggca ttgctctatt atagtataga acccgcctcg
    10381 aaaacagttt ttataaaagc aggaattccg gccggtccat cagggatggt ttaatgaaac
    10441 aaggacgctt gcggccacct ggcgcgtttt tcaacgggcg ttttcgcgcg ccatttcttc
    10501 cgcttctttc gcaatgtcca atccgcggag caggtaaagc agtccggtta tgattacggc
    10561 ggcgactatg ccgaaagtta tcatgccgat gccaataagg acgcctatgg cggccgtcgt
    10621 ccagacgagc gcggcggtcg tgatgccgcg tgaccggttg tcggttttaa tgatgagccc
    10681 cgcgccgagg aatccgatac cgacgacgat attcgcgata acctgaagaa acccgctgtt
    10741 tctggcaatg acgtcggcgg tattgccggt atttacggag acgatataag gaagtatgag
    10801 gccgaccatt gtgaaaattg ttgcgccgcc cgcgacgagc atttcggtgc ggatgccggc
    10861 ttctttgccg acgagctcgc gttcaagacc caagagcgcg ccgagaacga gcgcgaccgc
    10921 gaaccggatc atcatttgtg aaaaggtgag cattgtaata gtatgatacc ataataccat
    10981 aggacatacg catttgaaaa aaaatcataa gaaaatatat caaaccgaaa cggcttggcc
    11041 gattccttcg ttattttccg gttattttca tcgccgtagt cggactacgt ctcggaaaat
    11101 aatcggaaaa tgcctcgtac tcggcacaag tgcgtaagtc ctataaaatt gtcatgagaa
    11161 cgcttatcgg agatattata aaaaatgacg gcaaagaggt cgagctgtgc ggctgggtgc
    11221 aggcgcggcg cgatcacggg aaactggttt ttatcgatct ccgcgaccgg agcggattta
    11281 cgcaggtggt tttcgggccg gacgtcgcgc acgcgtccga actgcggctt gagtatgtgg
    11341 tgaaaataaa aggcggggtg aaaaaacgcc cgccgagcat ggtgaacgat aaaattccga
    11401 ccggcgcgta cgaagtcgga gcgacggagc ttacgatcct gaaccgtacc ggtgagatgc
    11461 ctttgcctat cctcggcgac ggatacgaca tcaacgaaga ggcgcggctg aagtaccgct
    11521 atctggattt gcgccgcagc cggatgaaag acaatctcat cgcccgccat gacgcaataa
    11581 aattcataag agattttctt tcggagcgcg gtttcatcga agttgaaacg ccgaatcttt
    11641 cgaagtcgac gcccgagggc gcgcgcgatt atctcgtgcc gtcgcgcatc cagaagggaa
    11701 gtttttacgc gcttccgcaa tcgccccagc agtacaagca gcttttgatg gtcgccggcg
    11761 tcgaaaaata tttccagatc gcgcgctgtt tccgcgacga ggacacgcgc ggcgaccgcc
    11821 agccggagtt tacgcagctc gacatggaaa tgagctttgt cgatcaggaa gaggttatgg
    11881 cattaaacga agaacttttg atcgcgcttg tcgcaactgc cgcgccccgt aaaaaaatac
    11941 aggagattcc gtttccgcgc atcacctaca aagatgcgat ggaaaaatac ggcaccgacc
    12001 gtccggatat gcggaaagat aagaacgatg aaaatcttct cgcgttttgc tgggttgtgg
    12061 atttcccgtt tttcgaaaaa acggaaacga ataccgcgcg atccgaagct tcggcgaaag
    12121 aatggacgtt cacgcacaat ccgttctccg cgccgaaacc ggaatttatg gatgacctgc
    12181 ttgcaaagaa aaatatccct gaaattttat ccgcgcaata tgacatcgcg atgaacggca
    12241 cggagatcgg cggcgggagc atccgcaacc accggcccga agcattggag gcggtttttg
    12301 aaatcatggg atttgaaaag gaaagtatcc ggaaaaattt cgggcatatc ctcgatgctt
    12361 tgggatacgg tgcgcctccg catggcggca tcgcctgggg attggatcgg ctggtatcgc
    12421 tgcttcaaaa cgagccgaac atccgcgagg tgattgcatt tccgaaaaca ggcgacggtc
    12481 gcgatcttat gatggatgcg ccgggcgaag tggacaaaaa acagctcgag gaactgggaa
    12541 tagagatcaa aaagcaatta taaaaagcaa tctccgcttc tgatctggtc gaggcgatct
    12601 ccggcggaca ccgtactctt aaaaaaataa aaataaattt ataaaaatat ggaacatcaa
    12661 aaatacgaac ggacattggt tatgataaag cctgacggca tccagcgcag cctgatcggc
    12721 gagatcgtca agcggtacga gcagagcggg ttgaagctgg tcgggatcaa gatgacggtg
    12781 ccgaccgtgg aacttatcga gcaacattat cttatcgatc ccgaatggcg tatgaagacc
    12841 gggctcaaga ccatcgagtc ctataagaaa aaaggcgcaa agcccccgtc cgaggatccg
    12901 cttgtgatca cggaaatcat tttgggcaat ctcaaaaaat acatgacgaa ggggccggtg
    12961 gtcgccatgg tgtggcaggg gatgcacgcg gtggggatcg tacgcaagat cacgggcggc
    13021 acggaaccct tgacgtcgag catgggaacc atccgcggcg acttcacgat cgattcgtat
    13081 gaagtgtcgg atatcgacgg ccgcgcggtg cggaacctca tccatgcatc cggttcgcag
    13141 gacgacgcgg aaaaagagat tgcgctgtgg tttacgccca aagaattaat cgactaccgc
    13201 cttatcaacg aagcgatgct ttacgatgcg gaactgagcg ggatactggg gcaataaatt
    13261 ttttttggag ctcattccga aaaacgattt ttttaggcag tgttcgcggg ttgttcggaa
    13321 attttatgaa actttgtttc atccgtcggt tcgtctggag gaagtaaacg cgcggacgcg
    13381 tttacttcct tatcgctgcc aacaggccgg caatttttgc cggcctgttt tttgaatcga
    13441 attctcatta cattaatgga acttgatgcg atacttcgtt tccgccccgg acgaagcttt
    13501 atcgcttgcg agggactttt aaggaagccc gtccctcgtt tccgcactag acggagcatt
    13561 ttaattcgga aaggagtaaa gagagcccct cctttccgaa ttaaaatgcg gagtccttta
    13621 agatatattt aatccgctcc ttgctatggt tattgaaata ggacttatgc gcttggccaa
    13681 gtacgaggca ttttccgagg attttgggag ccgtagtccg actacggcga tgaaaaccgc
    13741 aggaaaataa cgaagtaatt ggccaggccc ttgcggtttg aaacatctga cgtttttaaa
    13801 atattttttc aaacgcgtaa gtcctatgaa attcacgtgc gtatgcgcgt cgaataaaaa
    13861 taaccaatgg aaatatacga ggaagtcgcc gtcgataaaa agagaaaatt catccgtcat
    13921 gcgttgattt tcgcaggggt tttaacggcg ggcactgccg tatatttttt cgcgttcggc
    13981 gccggcagcg gttttttgaa aaataacgcg gccatcgccg tgcagtcgtt ccgtgaagca
    14041 ttcggtttgt ctttaagcgg aaatacaaag gaagttgcac tggagaatgt ttctgctgat
    14101 agttcgtcgt ctgtagaaat gagttcgtca tcaactgtga atgaaaatga tccggctggc
    14161 ggaacagccg atgcgaatat cgccggaaat gattttgcgc aaccaccggc aagtaaaaat
    14221 gccgccgccg cggtaagcgc gcaagccgac atacggcaat catccggcgt ggaaagcgtt
    14281 tccggtttgg aactggatgt cgtggcggca agcggcgcag tatcaagcac gatcatgccg
    14341 gatggcggtg atgttccggg gatgacttcc gatacttccg aaaatgcgac aggcagtccc
    14401 gattccgttt cggattccgt atcgggaacg gcatctttcg ttccgccttt cggagcgtcg
    14461 ttttcgacgt catcgctttt ggggagcaca tcttctttta acgggaggcc atcatcgccg
    14521 ccggcgcggc cgcgatgcgc gtttaccgcc gcgaatactg ccgcgccgtc gcatcggatt
    14581 attttgaacg agatcgcgtg gatgggatcg cctgcgcaaa gcggcgaaag cgcggcgggc
    14641 gcgggcaatg acgaatggat ggagataaaa aatgagacgt caagcacggt tccgctcgcc
    14701 ggctgggagc ttatcgacac ggcggaaaat ataacggtcg ttttcggtga tagcgaccgt
    14761 attgcgccgt catcgttcta ttttctcaag cgtacggatg acaatgcgat atccggtatt
    14821 ccggcggaca agatttattc cggcgcgttg ccgaatacgg gcgacgcgct cggcattttt
    14881 gacggcgcgt gcgcgcccgt ggatattttg gacgcgtcgg acggatggcc gggcggcgac
    14941 aatggcgcga agcggacatt ggaacgtaat cgcgccgatt tcggatggca tacgagcgcg
    15001 gatcccggcg gaacgccgaa agccgaaaat tcaacgggat atattgcgcc ggttgctgtt
    15061 gcgacttctt cggtgacgca aacgtcgtct tcggtatgtg ccgccaatga ttcttcgggc
    15121 ggtcttttga ggagtgatat taccgccgat ctatcgtccg tcgcggcgga tgcgtcttca
    15181 agttttgata tttccggcgg ttccggcggc ggcgatatcc agctatcgtc aacatcagcc
    15241 gtcgtattcg gcgacgcaac gacatcaacg gttgcgtcag gcgctgcgac gaccgccgga
    15301 gcgattgcgc atcttatgat cgtcgccgtg cagattgcgg gcgcatcgac aacggatgat
    15361 tttgtgaaaa tttataaccc tgaaaatagc gcggtcgacg tgagcggatg gaaattacgc
    15421 aaaaaaagcg gcacgggcgc ggattattca ttgagagaat ttccggcggg cagtgcggtg
    15481 ccggccggcg gcatttttac gtgggcgaat tccgcgaacg gattttcggc ggcgatcggc
    15541 gcggatgttt cgagtaccga gacgctgggt gcggataata gcgttgcgct tttcgatgcc
    15601 acgggcgtca tggtcgatgc ggtcgcgtgg ggaacgggcg tgaaccaata tggcgaaggc
    15661 gcggcgtatc cgaccgaccc cgcggcggac caggcgctca cgaggatttt tcaaaacggc
    15721 gcgattatcg atacggacaa taatgcggac gatttcacaa tccaatgatc cgtgatgagc
    15781 gttgaggtcg accggccatt caagggcggt tttgaatcaa cgcttctcag ttgtccccgc
    15841 ataaattctt taaagaaaat ttaatgcgcg gcggcataga atgaaagaaa aataaccaat
    15901 aaaaatattt tcatgcagaa tatcgaaaaa ccaaaaaaga cgggttcgta ttttattttc
    15961 gcggggatat ttatcgtgat ggtagcggcg gcggtcgttg tctttataat
//
LOCUS       DAIDDT010000012        15648 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_12_length_15648_cov_6.359964, whole genome shotgun sequence.
ACCESSION   DAIDDT010000012 DAIDDT010000000
VERSION     DAIDDT010000012.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15648)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15648)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15648
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12_length_15648_cov_6.359964"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4047 a         4242 c         3807 g         3552 t
ORIGIN      
        1 aacaatgttc tttgataatt aaatagataa aaaaataagg cctcgcggcc actcgccgga
       61 gagatatccg gtaattcttt aaaaggaggc aacaaatgct acaagatatt ttaattttcg
      121 cgatcgcgtt cgtcgcggca ggattaagtt cgatgtcggg cggcggcgcg ggggttattc
      181 tttttccagt cctcctttct atgggagtgt cgtatccact ggtcgccgcc gtttcatcgg
      241 tgaattccgc ggcatgggtt cttcctgcgg cgaggaatta tctgaaaggc gggaggaaaa
      301 tacattggcc actcgtcgcc atattttcaa tcgtgggact tatcggctgc tacgccggcg
      361 taatcttcgc gattgacgtg aatcagcgga ttctcgcaat cgtcgtgggt attatcatcg
      421 tcttcctggc agtatatacc tacttcagga aaggtttcgg acttagcgaa catcacgtct
      481 attcgcgctt ccgtcaggcg attgcatggc cgtttgcgct ggtcctcggg ttttacgaga
      541 cagtgttcgg ttcagcgaac ggcattatgt ttacatcgct gactgttcat acaaacgggt
      601 tcgattttaa ggatggactc gcccattact acctcatctc gttttcatgg gcattgttcg
      661 ccgcaatcct gttcattcaa cgcggatatt acgacattcc tatgatgatt ttcgcggcaa
      721 ccggatcggt tgcgggcgca tatctcggat caaggcatgt gcaaaagaaa ggcaacaaat
      781 ttataaagtt gcttttcgtg atcatcggag ggttgctcgg cctgaagatg attttcggat
      841 tataaaaata aagctggtcc ttcatggatc ggcttttttc tttttaaatt aaaattttac
      901 gaagtatatt tcccggcaac atgcctcggc ggcgcgggcg tggcgcaaca atgtgataaa
      961 ataaacccat gctcctcttt tcgctgatcc ttgcgcttgc gcccggcttc gcgtggctgg
     1021 ttttctatct cggcgaagat ccccgtcccg aaccgaaacg gcttatcgcg ctcgcgtttg
     1081 ccgccggcat cattgccgga cttttcgcca tcggcatcga attgatcttc aagcaggcca
     1141 tcagcgaaac cggcatcggc gaatactccg tcattgcgct catcgtattc gcattcatag
     1201 aggaattcct gaaattcggc gcggcatatt tcgccgtcaa taaggatccg cgcttcaccg
     1261 agcccgtcga cgccatgatc tacgccatcg tcgccgcgct cggattcgcc accctcgaaa
     1321 atatcggcac catcgccgcg atgccgcaac agaccgcgct tatcgccgcg atcttccaga
     1381 cctcgtcgct ccgcttcgtt ggcgcaacgc tcctgcattc cctcgcgtcg ggcgtcgtcg
     1441 gatattattg ggcaaaaggc atcatccgcg gcaaagtggt acgcttcctc gccttcggac
     1501 tcgcgctcgc caccgtgttg cacgccacat tcaattatct tatactgaac tatgagaata
     1561 tcgcttactc gatcatattc ctcggcgtcg tcggtctttt cgtactggtt gattttgaaa
     1621 aactgaaaga gaagccgatc gatatcataa gttgataaaa aatcacaaaa caaaccgtat
     1681 tataaaaaat aataattatc aatcgccatg gacgcatcgg aaaaaaattt ttttgaaaca
     1741 ctcgcggtcg cgaattcaag cgccgaagaa aattacggcg tagagttcgc gaaagccgaa
     1801 accggcgcca tagccgccgc gcgctcgaaa gccgccgaac cgaagatcgc ggaccaggac
     1861 gaggcggaag aaaaatggga cgacggcgag ccggaaggga agctcatggt tgacgtctat
     1921 cagacgccgt ccgagatcgt catcgaatcc gcgattgcag gcacaaaacc cgagaacatc
     1981 gacgtcagcg ccacgactga ttccgtaacc atcagggggt cgcgcaaacg ggaaaaagaa
     2041 gcgagagacg aaaattatct ttatcaggaa tgctattggg gaaaattcac ccgttcggtc
     2101 atcctcccgc aggaagtgga tccggaaggc gcgaccgtga cgttcaagaa cggcgtcctc
     2161 acggtccggc tccccaaagt gaacaagaaa aaaacgaaaa aactgagcgt gaaaacggag
     2221 taaaatccgt tttaaaagca ggccagcctt ttattctata caatagaata tacgcgcttg
     2281 tgccgcgcac gagacatttt ctctccgtcg tccccccgaa ggaaaaaatc tatcccctct
     2341 gtcgtcctcg cgaagaggag gatccatgct tccaatcttt tttcttaatc actcatcgct
     2401 ctcttcctca tcgctacttc tttattaaag cacggatctt cgcctccgcg aggacgacga
     2461 aatggaccgt aagaggttat ttccagacaa ttgcttccat gctgcttgca aaaaagcggc
     2521 gacactgtta tactcgaccc atcaaatgaa aagcggccgg tggaaatgca cgataccgga
     2581 cgccgccaat gaccgtatac tatcactacc atgagaaaat gcgcacaatg cgaaaaggga
     2641 tcaaaaatgg tcggccaccg gcggcttctc cgcggccatt acaatccgac caactggacc
     2701 cgtaaatatc ccaacatcca atgggcgacg atcaagggca ttcgcacgcg catctgcgtg
     2761 cagtgcataa aatcgctcca taaagctccg cataaaacca caaaagcagc agtcacgaaa
     2821 taaaacatat cccatgtccg gattcgaaaa gccaggaatc gatccattga tagaacgcga
     2881 agaggaaaaa gaagggatag aaaaacttaa aagttttgtc gaaattttga cggagccgta
     2941 ccgcgccgaa ggcatccccg tggataaaag cgggcgcatt gatatgcgca acggcgctta
     3001 taaggccatc tatcccgatg ttgaacagga ttttcaacgc acaagagaag gaggcaccga
     3061 cagcgcggcg cttttggaaa aaaaattaca caccgacggc gaaaagcttg agatgctcgc
     3121 ctatgccgtt ttcacaaaaa acctcagcgg gaaattcatc gtgatgcgct cggcgccgta
     3181 cgacgataaa aaacacaatg tcgacacgct gatattcgac cgcgaaacgg ggaacttggt
     3241 atgcgcattc gacgaggtcg gcgacacgac gggcgacgcc tacgagaaga aaaagaactt
     3301 ggtccagaaa cgtaacgttg acgatggcgg cgcggaactc aaatacgcac tccgcctcga
     3361 cgaaaaggac ggaaaaaaaa cagccgtgcc gggcgccgcg gaccatatcc ctcttttcta
     3421 tatcgccctc cctaaagaca ggatcgaaaa aggaataagg gaattcgcca aagggcagga
     3481 acaatcggaa ttcgaaaaaa ggctctttaa atattttatt gcaacgctcg gcgcccagat
     3541 acaaggcctt gaactttata gaaaacggct ggatcccgca ttgaggcaaa aacttgtcgc
     3601 attcaaaaag atagtaaacg acttagatca aagatacgag aaagagtaaa acccgtctca
     3661 aaagtagttt ttctaaaaat ggtccgcagg tcttgcgtcc atttccggct gcgcaccgcg
     3721 attccctcct cggaagacat gagtttctct cgttaggaga tcacgccgct cgctcggaaa
     3781 ttttatgaaa ctttatttca tccgccggtt cgctctgaaa aaatgaatgt gcgaacgcat
     3841 tcatttttta tcgctgccga cggccggcaa tccccgctgg cctgcttttg atacgagttc
     3901 tgggttcccg tagcttccaa tatgccggcg atcccgcaaa aattcacctt gcattcgtcc
     3961 ggccctgcaa gtattctgaa aaggtgctgg ataaatgcgg gctgtagtat accggcagta
     4021 cgtgcgcatg gggtgcgtaa agaccgggtt caattcccgg cagcccgaca tagtgggaag
     4081 tgctttaatt ttttcaaaac caacgcgctc gcttcgctcg ctgtcaaccg cacgataatt
     4141 ttttttccgt gcaatatggt atactgtgaa tatggaaaaa tatatatgga ctcccgacta
     4201 tagcgtaggc gtcacgctta tcgatgaaca acaccagcat ttcttcgaga ttgcgaataa
     4261 tatccttggc ttcgcggatg ggaaagcggt ggaaaaggaa atgctcttca cctatctcgg
     4321 agaactcggc gattattctt tttatcatct tggcacggaa gaaaagtttt ttgacgaatt
     4381 caaatatgaa ggtgcgcagg tacaccgggc cgcccatgac gttttccgcg ggataacgaa
     4441 aaaatatctt aacgacttgc gagatccggc atccgacacc gccaagatag cgcgcgcaat
     4501 ggcgatttat gcgggcgact ggctcaaaga acacatcctc gtaatggata aacaatatac
     4561 cgccgttttc cacgaacacg gattgcgata aaacaccgca tttttccgga tgcggtgttt
     4621 ttctttttcc ttttttgttt catttcacaa aaagattttc accgcccccg ataattttca
     4681 cggttgcggt ttttttgaaa cgaggcaaag aaacagaaaa atcggcgctc gaccattcaa
     4741 caaaaaatcc ccgctcacgg caggggttct ttgtaaatcc cgatagatct caggcgtaac
     4801 gccatgtggg tcggctcaat tgcgatccaa gaatcgcaat gattcgccat cccttttgta
     4861 attgtttgat ccgagatcta atacgggatc tgctttcagt atagcaaccg ttttccgcct
     4921 gtaaagtgga taactttccg cgcaaaaaag gcttttatcg aaacgattgt ttctataaaa
     4981 gtatctttgt cttttcaata tcctgtacgt agtacgtatc ctttcaaaat atccttagat
     5041 gcgctattat agatgtttct accgtatatt ccatgaccct gaaaaaaaga aaactgatat
     5101 tttatctcct ggtcgcatta tttttcgtca tcggcgccgc cgtcatcctt tatgcgcagg
     5161 gatggcggtt cgatgcggca acgtggcgcg cgagcaaagt cggcggcatt ttcgtccgat
     5221 cattccccga tgacgcccgg atatccctcg ataaaaaacc cattcaaaat gattcctcgt
     5281 tccttggacg cggcacgctc attgcaaacc tttttccgaa aacgtacgac cttgaacttg
     5341 aaaaaaccgg ctatgacgca tggcacgaaa acgccgcggt gtccccttct ctcgttgccg
     5401 cattcaaatc cgcggtgctc gtcccgcaaa atgcgacttc ggtcgcgcag ggacccgtac
     5461 aaaaattttt cgccgcgaat aatacggtag tagtccaaaa tacgaatggc gcgctgtcgt
     5521 ccgacggcct gccaatcggc aaaggaacgc ttatcgatga agcgaaggat ggcagcgccg
     5581 caatcataaa aaataatacg aacggaaaat attcgctcta ccaatccgga aacggcggca
     5641 caaccgacct gagcgccatc ctcgcgaaaa gcggcgcggc gaaaggcgat gttgcgagca
     5701 tctttttcga tccgcgcgat ccgggaatga tcgtcgcggc gggaccgtcc aagatatggc
     5761 ttatcgatac cgcgaatgca aatacaacgc tgattaagaa tatatccgca gccgcaaccg
     5821 caaaaatatc cgcaaaagaa attatcggcg atagcatcgc cgccggaaca aacatgatcg
     5881 catggacgga atcggaaaaa tcggcgcgtt cgtcggatat aatgatttat gatacgtccg
     5941 cggaatcgat agccgcttca tccaccctgc ccggacaaaa tagcgacctc aaatgggtga
     6001 aagacggcat gctcggcatc cttcagaacg acggcgaact ttatctttac gatacgggaa
     6061 cggacagcgt cacgcatatc gccgacgacg tgaaatattt cgcgccgacc gcggacggat
     6121 cgtccgtcgc ggcgctcgaa aacaaaagta tggagatcat cccgctcacg ggcaatagtt
     6181 acgattacta ccgcttcaat ctgccggata ttgcggatgc gcggcgcgtc atctggtata
     6241 aggacaccat gcaccttttc atagaatacg gcgaccgcgt ttcgttcctc gacctcaaag
     6301 acgccggcct cgacaacttc acgaccgtgg cgcagggcat ggcgccgttc tatgacgccg
     6361 acaacaacgc gctgtatctt atcaatcccg cccaaaatct tgtccgcttc gattttccgg
     6421 aatagaaaaa gatctgcgat ggcgtcgaca gggccttacg acgctgtctt tttgatcacg
     6481 cttttgtaat atttaaattt taaaaaatcg attctatgtt ctctcttccg tttcctgtat
     6541 actttgcatt atggattatc tcataacgga aacagccgga tgcgacggct atgaactttt
     6601 ggacagcggc gacggagaaa aactggaacg gtacggcggc gtcatcatgg cgcggcccga
     6661 tccgcaggcg ctctggaaaa aacggctgcc gcacaacgaa tggcaaaaag taaaagcgcg
     6721 tttcgccatc cccggtcttg tcgttaaaaa ttttacggaa caaaaatcaa ggggacgaac
     6781 ccgtgcggca gcgaacgcac aatgggaact cgcgtccggc gtcccgcaga aatggaaaac
     6841 cgatttcgcc ggcctttcgt tctggatacg tccgaccgca ttcaaacaca cggggctgtt
     6901 tccggaacac gcgccgaact ggcaatggat cggcgaaaaa atcagcggcg cgcggcgacc
     6961 cgtttccgtc atcaatcttt tcggctatac gggcggagcg acgctcgccg cggcaaaagc
     7021 cggcgcggcc gtgacgcatg tcgacggctc gcgcgtcgcc gtcgcatggg cgcgagaaaa
     7081 cgcggaggcg tctggattaa aagacgctcc catccggtgg atcgtcgacg atgcgcgcgc
     7141 ttttttgaaa cgggaaataa aacgcggaaa acggtacgac ggcatcatta tcgatccgcc
     7201 cgcgttcggc cacggatcgg acggcgattt gtggaaaata gaagatgatt ttttgccgtt
     7261 tcttgatctg tgtaaaaccg cgatgacacc cgcgccgctt ttctttttga tgaacggcta
     7321 tgcctccggc tattccgcca tcgcttataa aaacaatctc gaaatgctta tgcgcggcgc
     7381 gaaagggacg attgaaatgg gagaacttac catacgcgaa accggcgcgg acggacggct
     7441 gctcccctgc ggcatcttcg cgcgatggag cgaataaagc tccccgcggc aaagccgacg
     7501 gggcgtccgg aaataaaaac agcgaacaaa aaaatgatgc tgcttaaaat cctttacgaa
     7561 gacaaccacc ttatcgccgt ctggaaaaat gccggcgttc tcgcgcaagg cgaccgctcc
     7621 ggcgaaacgt cattaatgga tgacgtcaaa aattacatca aagaaaaata taaaaaaccc
     7681 gggaatgttt ttctcggact tctgcaccgg ctcgaccgtc ccgtcgcggg catcacgctc
     7741 ttcgcaaaaa caagcaaggg ggcaagccgc ctctccgaac agttccgcga acaggcggtc
     7801 aagaaaacat attatgccgt cattgaggga cggttgccga aagagagcgg ggcgctcgtc
     7861 aatatgatcg aaaaaaggga aacgatgtcc ggcggacgga aggcgaaaat aacaaaaaac
     7921 agcggcgacc gcgccgagct tgcgtacgaa accgtgcggc gcgggcagcg ctattccctt
     7981 ataaaaatac gcctcaagac cggcaagttc caccagatca gggcgcagtt cgccgcggcg
     8041 ggatgtcccg tcgcaggcga cataaaatac ggcgggcacc cgtccccgtt tctcacaaac
     8101 ggcacgattg cgcttgccgc atgcgaattt tccttcacga ccgcaaccga aaataaaacg
     8161 attgcactca aagttccgta cccgaaagaa tggaacgcgc cgacaaggga atagcgaaat
     8221 atttcgagga gaaatccacg ctttccagac aaaaaaaccg caccgctctc gggcgccgtt
     8281 tgtaatactg cttcgaaacg ggatctctgc ccatccctgc gcaagaggcg gcatattcgc
     8341 cttaaaacgg gataaaatta tttttctcag caagcaaata cttcctgtta caggagacgg
     8401 aattttaact ttcaaccacg ggcgttcgtt ccgaacgcgt ggtccatgga aaagaaaacc
     8461 ataaatccaa acgcggatcc aaaaagaaga ttcaacaaga acgacggcga tgcggtaata
     8521 atggactgcg gatcggcgat gagataaaaa ataacgaatc cagcgaaaag cgaaatgatt
     8581 gttcctgtcc acggttccca cgggaatgcg atccgggaaa aatacgccgc gcagggaatg
     8641 atggcaaaag caataatggc gatactcggc gccggctgcc agttcattat gaaaacgccg
     8701 agcaggacaa aaaacagcaa ttcaaaaaat ccgccgcggc gcgggaaaac gaatgcgaac
     8761 gctatgagcg ccgcgaaaat aagattgacc gatcccgccg gcgcgaaaga gaactgcagc
     8821 acgcaggcaa tggcgaagac gaaaattccg gaaaaaaatc gtaaattcat gttgtaatgt
     8881 tgtagcgttg taatgaacta tacgaaacat tacaacctga ttttatttct caatggccac
     8941 cgtctggata ctgtttatat catacggaaa atcaaccgtc gcttcctcga aaagattgtc
     9001 cgccgaaacg gcaacgtcgc ggaccgtgcc tatcggcgcg ccgtacggaa aagcgggatc
     9061 ggcgcttatg atgacgtccc cgcttttcac ggcagcggtt ttgatgatgg atcccgcctt
     9121 cggctccgta ccgcctgtca aaagcgcgtc atatcccgcg gaacccacgc gtacgggcat
     9181 tttgaaatcg ccgtcgaaaa ccgtctgcac gagcgcggta tccttaaaaa ctttttcgac
     9241 cttaccgata agcgtagtgc caaagaaaac cgccttgccc gccgtcacgc ccgactgcgc
     9301 gccggcattg acgagaagct cgttcctgaa attcatcgga taccgcgaat agaccatcgc
     9361 gcggatggtg cccgagggcg caacgggcaa ttgggattgg acaacctgaa gttccgcaag
     9421 ctgcgctttg agcacgatgt tttccgaggc caaaccgttt ggatcgatcg gaggttcatg
     9481 tgtttcgccg agccattgcc gcacgcgcca tccgtatgac ggcgcaaaaa aaataagcag
     9541 caaaaggatg gtgatgtaca gacttaaaaa ccaattcttt tttttcatag gcgccccgtt
     9601 cacgatctaa tactaatttc tcgcacctct gtcgtcctcg cgaaggctcg ccctatggaa
     9661 tggcggcttc gcctactcca tagggcgagg atccacgctt ttagaatttt ataatccgtg
     9721 gctcacgatg cacgaattaa gattggaagc atggatcctc ctcttcggga ggacgacaga
     9781 tctataagtc aaagattgat gacaagcggc tttaagggat tatcaagaag ccctcggtgc
     9841 gattggaaat catcaatgat gataccgagg ccgcgcgcga cgcaaagcag cggatcgtcc
     9901 gcaatcctga cgggaacgcc ggtctctttt tgcagcagat ggtcaagccc gcgcaggagc
     9961 gcgccgccgc cgcataacgt gatcccctgc cgcagggcat cgccggcaag ctccggcggc
    10021 gtttcctcta tggtttcatg aacggcatcg atgatcgtgc ggagtgattt tgcgatcgcg
    10081 gcgcggacat gattgttctt tatccctatc tcccgcggca atcccgtgct catgtcgcgg
    10141 ccacggatcg tcacttccat cctctcgtcg agcggaatcg cagatcccac ggcgattttc
    10201 gcgaactcgg cggtgggttc ccctatcgca agatggaact cgtcgcgcac gaaccggatg
    10261 atgtcctcat tgaatcgatc gccggcaact ttcagggttt ttgcgatcac cgtgccgccc
    10321 aaagaaatga cggcaatgtc ggtcgtgccg ccgccgatat cgacgataac gctcgcggcg
    10381 ggcgcgtcga tcaaaagccc tgcgccaagc gccgccacaa tcggcgattc cacgagatac
    10441 accttcgaac agccggcatt caaagcggca tcctcgaccg acttctgctc cacttccgta
    10501 agatgattgg gaatcgcgag caccgcgcgg cggtacgaaa caatcggatg cgattttgaa
    10561 cggttcaaaa gatgccgcag cacttcctcg gtcatctcga aatccgaaac gacgccgttc
    10621 acgagcggac ggacgacgga aatgtgcgcc ggcgtcctgc caagcatttt ttttgcatca
    10681 tcgccgacgg caaggatatg gttcgtcttc gcgttcatcg cgacgaccga cggctcgttc
    10741 acgacaatgc cgcgatcttt gaaataaatg actgcgttcg ccgtaccgag atcgatgccg
    10801 acatctttga aaattgaatc gagaaaagcc atgaaaaatt ttagtgttgt aatgttttaa
    10861 agttgtaatg aaaaatggaa cgcatgagaa aataatttaa gttcttgccg aaaacattat
    10921 catcttcacc gcttcgccta cgtccaccac ctttgattct ttttcgccgc ggcgttttat
    10981 ttccacctta ttttccgcga ttttcgggct cacgacaagg cgccacggaa tgccgataag
    11041 gtcggctccc gcaaacttct ctcccgccgt cataaccctc tcatcgtaaa gtacttcaac
    11101 gccggccgtt tgtaaagttt tataaatata ttcgccgtcc gcgccgttca gggcaagcag
    11161 gtgcgcgcgg aacggcgcga ccgattccgg ccacacgatg ccgtttgcat cgttatattt
    11221 ttcgacgatc acgcccataa gccgcgagac gccgatgccg tacgatccca tcacgacata
    11281 ttttttttcg cctttttcgt cgtcaaaaga aagattgaac gcttcggaat atttcgtgcc
    11341 gagcgggaaa atattgccga cctcgatggc tttttcctct ttgatgattc cgccgcactg
    11401 cggacatttt tccccgtctt tcaatttcga tatttccttg ttctcggcat atccgcattg
    11461 cccgcaaaca tagattgtgt cttcgccgat atccgaagca acctggaatt cgtgcgtgtt
    11521 gctgatcgtg aaatcaccgc cggcggcgac cgtgacgaac gcctgtaggc cgcaacgccc
    11581 gaagactttc ttgtacgccg gcaccgccat cccgtgataa aacttcataa gatcctcttc
    11641 cgaggcgtgg aaactgtaca tgtctttcat caaaaattcc cgtccgcgca gaagccccga
    11701 tttcgcgcgc ggctcgttcc tgaacttcgc ctgtatctga tacgccgcga acggcagatc
    11761 tttatatgag gaaacatatt tcgtcgcgat ctccgccata atttcctcgt gcgtccagct
    11821 cagattgaac gacggctcct tccccgctcc ttcggcaacc ttgaacacca cgtcgacatc
    11881 ccatcgcccc gtcgctttca gatatttctt gtcgtgcagc gcggccatca gcacttcctg
    11941 tccgccgatg gcatccattt cctcgcggat gatcgcctga atcttccgca tcacgcgcca
    12001 cccgagcggc agaaaagaat aaactcccgc cgaattttta taaataaatc cggcgcgttc
    12061 gagcagctgc gcattcaacg acacctcttc tttcggcgcg ttccgttccg ttttcgtgaa
    12121 taattccgac tggcgcatag cgaataatac atccatgata gcctgttttc tttattttgt
    12181 catccgcgcg gatggctttg ttgggcgcgt ttgaactcgc cggcatcgca cgccggaaga
    12241 agggtacctg acactgttct ccccgcgcgc gaaaactcgg gataagctcc ggcgaagatc
    12301 catcccctct gtcgtcctcg cggaggcgag gattcacgct ttaaaaattt tatgatgcat
    12361 gctttatgaa ttaagactca gtcggggggg gagaaaggaa agtctcttat attatccctg
    12421 ttccagttcc gcttcgataa accggtctat gtcccccgcg agtacgtcat caacccgcgc
    12481 cgtttcaaca tcggtccggt gatctttcac gagctggtac ggattcaaga catatgaacg
    12541 gatctgattc ccccattccg gttttacttt cgtccggaga ttgccgagct cggcgacttt
    12601 ttctttttcc ataagacgga acaatttcgc tttcaaaagt ttcatggcat attcgcgatt
    12661 ctgcgcctgc gcccgctgaa cctgcgacgt taccgtgatg cccgtaggaa gatggattac
    12721 ccgcacggcg gtttccactt tgttcacgtt ctgcccgccg ggacctcccg aacggtaaaa
    12781 ttcgactttc agatcctttt ccggaatctg cattttcgat tcctcgagcg gcggcagatc
    12841 gggcaccact tcgacgagcg cgaacgatgt ctcgcgcgaa tgtttcgtat taaaaggcga
    12901 tatacggaca aggcgatgta cgccggattc ttttttaaga taaccgtata catgcccgcc
    12961 gcgaagctcg acggtccgcg aacgcgggtt atcgtcgatg acttttattt tccatccgcg
    13021 attgcgggca taattttcgt acatcaacgc caacatccgc gcccagtcct ccgcgtcgtc
    13081 gccgccggct cccggaaaaa cggaaagtac ggcagcctgc gcgtcgtacg gttccgtaaa
    13141 aagcctgtcc agttccagtt ctttaaattt gtttttgatt tcgtaaaatt tcgcttcatc
    13201 gaattttttc cggagctcgc cgatgcccgt ttcgatcgcg tgataacgcg taagaatttc
    13261 ctgcgcctcg cccagttctt ttatcttgag atcggcctcg gcgcgattcg tccaaaacgc
    13321 cggatccgcc atggcggcgg ttaatgcact tacgcgcgat gctttctccg cgacctcaaa
    13381 gccgcccgcc taaccggcgg gcttcgattg caagctcttc aagttctttt gcgtaatcca
    13441 taaattcgaa tgattatttt ttttcgcctt cagacttcaa gaaagcggtt ttaattttac
    13501 tgttcctgca tacgtagcaa ttcccataac ttgcatacgc gttgccgcac gcggaacatt
    13561 cgccgtcctc tttttccggt aaaaggctct ctattttttc cgcatcatgc gcgggcggtc
    13621 ttttttccga cgcctgaggc ggcgtttccg atttttcgaa accgaagctt aactgcctca
    13681 taagtccatt atatcatatt ttgcctgcgc gcggcgagcg ggattttcaa acaagcccga
    13741 atttcggtag aatgaatcca tgtccatcaa agaaacggta aaaaaagatc ctcaaggcgt
    13801 gattgcgcgg gcgtatgttt tcgcagaaaa aacgcaccgt ggacagaaac gcaaaagcgg
    13861 cgaaccgtac ttcaaccatc cgcttgcaac ggcgaatatc ctgtcaagct ggcgcctcga
    13921 taacgcgacc atcgcggcgg gattactcca cgacaccgtg gaagatacgg acgtcacgca
    13981 ggatgatatt cataaagaat tcggcggcga aatcgcgttt cttgtcgaag gcgtgacgaa
    14041 actcggcacc atcaaatacc gcggcgcgga gaataaggcg gaaaatatga gaaaaatgat
    14101 tctcgcctta agccgcgacc tgcgcgtcgt ctttattaaa ctcgccgacc ggcttcataa
    14161 catgcggaca ctttcggcgc tgccgcccgc gaagcaaaaa agaatcgcac tcgaaacgaa
    14221 cgacatctac gcgccgctcg cctaccgtct cggcatgcag aacgtgagcg gcgaactgca
    14281 ggatctcgca ttcccctatc tccatccgaa agaataccgg tggcttcttg aaacaaccaa
    14341 ggaacaatat gaatcgcggc tcgcatatct tcaaaaaata aaacccgagg tcgaagccct
    14401 gctcaaaaag aacggcgtgg cgccgctcgc gatcgatttc cgcgcgaaac gctacagcag
    14461 tctgtataaa aaactcctgc ggcacgatat ggacatcggg aagatctacg atctcgtggc
    14521 gatgcggatt attgtcgcaa cggttcccga atgctatgcc gccatgggaa tcgtccacga
    14581 acaatggccg ccgcttccgg gacggataaa agattatatc gccatgccga aaccgaacac
    14641 ctaccggagc ttgcacacga ccgtcatcgg cccggatcaa aaaataatgg aattccagat
    14701 acggacgaaa gaaatgcacg aagagaacga gtacggcgtc gccgctcact ggctctacaa
    14761 gcaaaaatcg gacggaaaaa cgaactcccc gaaaaccgcc gcggaagaca tcgcgtgggt
    14821 ccggcagctg caaagctggc aggaacattt ttcgggaaaa caaacggaat ccgaagaatt
    14881 catgcggacg atgaagatcg atttcttcaa ggagcggatt ttcgccatca cgccccgcgg
    14941 cgacgccatc gatcttcccg ccggatcgac gccggtcgat ttcgcatacc acatccatac
    15001 ggacatcggc aacgcgtgcg ccggcgcgaa agtgaacggc gcaatcgtgc cgctcgatca
    15061 cgagctccgg tcgggcgatc ttgtcgaaat catcacgcaa aaagggaaac gcccttcgga
    15121 aacatggctt acgttcgtaa agaccgcaat cgcgcgcgac cgcatccgcg cggctatgcg
    15181 cgaaaaaaac cgtcttgtcc gcggcgcggc ggcacccacg cacgcggaac tcaaaatcac
    15241 ggtcgacgat cgtgtcggca tacttaaaga tttttcgacc atcatcgccc gcaaccatat
    15301 caatatcctc gccttccacg ccgaaaacac gaaagggagc cgcttctcgc tgaataaaat
    15361 agatatccaa tccatcgaca aaccgaaaat cgacaaactg atcctgaaat tgaaaaaagt
    15421 ggacggcgta aaagaaataa gctacaaact cgtctgaaat attgtcggcg cggattcttt
    15481 cccttcccgc gccgcggcga cgcaggaagg gaaagaaaat tttcatttta tatgttactc
    15541 aaatatcttt tctgcgctga aagtgccacc gcgatctttt tctaaaataa aaaagggcga
    15601 caatccatgt cgccccgtta aaaaaactat ttaacattaa ttcaaaat
//
LOCUS       DAIDDT010000013        15088 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_13_length_15088_cov_6.072280, whole genome shotgun sequence.
ACCESSION   DAIDDT010000013 DAIDDT010000000
VERSION     DAIDDT010000013.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15088)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15088)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15088
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13_length_15088_cov_6.072280"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3570 a         4263 c         3408 g         3847 t
ORIGIN      
        1 tgcatgaata tccataccac cgccgttaaa taaggcagtg actttcaaga aaacaaaatt
       61 gttttccgaa atagataaat ggcataatag acttccgagg gaaccttggg gagaagtata
      121 gcaaaaagga cttcttgatg tcaatacata tttgtcgccc tcgcgaaggc gacagagaga
      181 tttgattttt gacgattttt taaaaagctc ttatttgcct gcaaaacgct accgatcagc
      241 tgacgttttt cacgaatacc gtaagccctt tccgtttttt ataaccgcat ttttcaacga
      301 acggaaaatt cttccgttcg gtctcgcagt aaaccggcgt cccttttgca agcgccgcta
      361 tctttttcat catggcacgg ccgatgccct gtttttgata atcggggtcg atcgccatgt
      421 cgagaatttt cgtatcaagc gcgaagtcgc tcaatacccg cgcgataccg ataagttccc
      481 ccgcttcgtt ccgcgccgaa accacaatgt cgcaatgagc aagcgaccgc ttcatacgaa
      541 ccgcggaata ccggcgcgcc gttccccatc cgagcgcgac atacaacgcc gccgcttcgt
      601 cgggctttac cgattttttg tcggttgaaa taaatatgca ggacataaaa tccattatat
      661 caaaacggaa atattcaaaa aaatgaaagt atggatcctc gctcccggaa atttttcgga
      721 gaaaaacttg aaaaagtcct ctctcgcgga gacaacggaa aaattttcgg acaactttac
      781 cgatagctaa atttatagat gctaaatttc tttttccaat tcttccagta tctttttcgc
      841 tttcgcgccc ggttttttcg gcgctttgat cataaaattc acgagcagat cgccgcggcg
      901 gtgcgtcccg atttgcggca tgccttcgcc cggaatgcgc aataattccc tgagattgaa
      961 tccggcggga atctcgatct gaatcttacc gcccgcgatc gtggaaattt ctactttttt
     1021 gccgagcaga agatcaagcg cgtgaagttc gcgcgcaacc acaaggtcgg ccccgtgccg
     1081 ctcgaaaacc tgatgcggcc gcacgcggat gcgcacataa agatcgccgg cggcggtgcc
     1141 gcgttctcct gcttcgccca tgccgttcat ttttatcagc tggttatcct cgacgccggg
     1201 caggatagtg accttgatct cgcgggttga ttccaccctt cccgatcctt tgcacacgtt
     1261 gcacggatgt ttcggcaact ctcctgcacc atgacacttt gcgcacgtct tcacctgcga
     1321 gaacgcgccg aaaaacgacc ggcgctgctc ccgtatctcg cctctgccgt cgcaaacgga
     1381 acatttttca aaactgcttc ccgcttcggc gcctttgccc ttgcattctt cgcatcgcac
     1441 gaacgtcctt atttttaacg tcttcgcggc gccgcggaaa acttcctcaa gcgtgatcgg
     1501 ttcctgcact tcaagatcgc tgccccgttc gtatgttttt ctccgcggct ggccgccgaa
     1561 cccctcgaag atgctctcga aaatatcatt gagatcgccc gcgccggaga aattctgcgg
     1621 atcgaaaccg aatccttccc agttcggctg tccgccgccc tgaaaccctc cccattgggc
     1681 gccattgaaa cctccggcgc ccgccgccgg tccggcactg ccgaaacggt cgtattgcgc
     1741 gcgcttcatc ttgtcggaaa gcacctgata cgcctcgttg atctccttga atttcgcctc
     1801 attaccgccc ggcttgtcgg gatggtgttc gtgcgcaagc ttgcggtatg ctttcttaat
     1861 ctcctcctct gacgcgtttt tcgcaacccc taaaatagtg taataatctt ttggcataaa
     1921 aataatttaa agtgatttca ttataatacc cgcatattaa aattttttaa agacatcctc
     1981 ctctgttgtc cccgtaaagg ctcacctgca tagagcctgt ctcggttttt ctccgaaaaa
     2041 ctttggcggg cggaaacccg tgcttcccgt cttgttcttt ttattttttt caaagcgtgg
     2101 gtccccggcc ggaaccggag acgacggaga aaaagaaatc aatattcaat gacttccttc
     2161 gtccatggtt cctctttaat ccgtatcaag cggaacacgc gaagtatttc gtagaaaata
     2221 aggagtagta actggtcgat ccagacgaag ataattcccg cgctccgcag taaaaagaaa
     2281 acagcaatac tccatccggc gagaaacgcg ccttggaacc gatctttcca cccggcaagt
     2341 gtattgacga taagccgata aacaaccgcg ctcacggtat cggacggctg aatcgcaatc
     2401 ttcatgcttt ttgaaagatt attaaggacg gccgacgcgc tttgtgttat cgccgcagcc
     2461 tgatccatgg ggctcatgct ttgaaacgcc ggcacggtat caagctggga acttacaata
     2521 ccattcgcaa attctccgaa agaacccgaa atgttgacgt tcggataaaa tgcgttcaaa
     2581 gtgcccgaca cccatccgaa aaacacgttg aaattctcct gcgagatgaa agcactgccc
     2641 ggctgccata aaggaagata aatcacaatc ataaaaataa tcgtgccagt caggacgttc
     2701 cctatcacgc ctctggttgc cttgaacaga cggatatcca tgccataatc gatctccgac
     2761 cggctcttga gatatcccca gaaaaaaaga ataaaggcga ttgcgccggc cgcacaaagg
     2821 aaacgccaat cgacggcata gaaaaatatg ccgagaaccg ccacctcgca cagtatgatg
     2881 aacgcgcggc gattcgtttc cttgcacagg agcgcctgga gcgccgaaac ggctccgaat
     2941 accaaaaatg ccgcaaagat ggcccatggc gacaaaccgt tcgcaagata ttcgcggtac
     3001 gaatatccca aaacgaatgc ggcaaggata cttgaagcgt caagcgctat caatttataa
     3061 gttccccgcc ttttcttctc aaccgaaaga gcaagcgatg ccctggcttg atcgataaga
     3121 ttctgtccct ggtccatctt gttagaaaat ttaataaacg ttttccgata tgaaacaatc
     3181 gtttgcaaaa acaaccgtca aatttgaaga ttccgtgatt ttaacaggat gtacgcgctt
     3241 gtgccgggta cgagacattt tctctctgtc atcgtccggc ttggccggac gatccatact
     3301 ttgaaaaatt aagaaaaaag attgaaagca tggatcccct ccttcgaggg gacgacgcaa
     3361 caagcattgc gccggatcgg gatcgtgaat cgtccgtcgg aatttaccct cgaccacgat
     3421 cgaggacgaa cgatgacaga ggagaaagtt tttatgattt tttcaaatgc gtatgtcctg
     3481 attaaatttt ctatcgggat tcgtttccgc catctttgtc gtcctggcta tcgccggccg
     3541 gaacgtcttt attatcttcg gttttattat cctcggcagg attttcctta ttgtagtacg
     3601 attggcctat tttttgaagc tccgcggaaa gcgcttcctg cgcgcgcatg atggcatcct
     3661 tatcttcgcc ttccttcgct tttttaagtt cctccaattt ttccgtaatg gatgttttca
     3721 cgtcggccgg caccttatcg ccggcgtcac gcattgattt ctcggcaaga tagatcgtcg
     3781 attccgcctg gttgcgtgca tcgatcagtt ctttcttttt cgcatcctcc accgcatgtt
     3841 cggcggcttc ttttttcaat ttttcaacct cgtccttcga aagattggtg gacgcttcga
     3901 ttttcaccga ttgcgtcttg cccgtaccct tgtccttcgc ggaaacattc agaatgccgt
     3961 tcgcgtcaat atcgaacgtc acctcgatct gcggcaggcc gcggggcgac ggcggaatgc
     4021 cgtcaagaat gaaccgcgca agcgtcttgt tgtccgatgc catcggccgt tcgccttgga
     4081 caatatgcac ctcgaccgag gtttgattat cggccgccgt tgaaaacacc tgcgacttcg
     4141 atgtcggcac ggtcgtgttt ttcggaatca tgggcgtggc aaccgcgcca aaggtctcga
     4201 tggaaagcga aagcggcgtc acgtccaaaa gcagaatatc tttcacgtcg ccctgcagaa
     4261 tacccgcctg caccgcggcg ccgagcgcca ccacttcatc gggattgatg ccctggtgcg
     4321 gatctttgcc gaaaagtttt ttcactgctt cctgcaccgc cggcatcctg gtctggccgc
     4381 cgacgaggat cacctcgttg atgtcggaaa ttttgaatcc cgcgcccttc ggcgctgcgg
     4441 ccgtaacggt ctctttggta agttctatag agtggtcgat atattcctga acaagcgatt
     4501 caagtttcgc ccgcgaaagt tttataagga aatgcttcgg gccgttcgcg tccgaagaaa
     4561 tgtacggcag atttatttcc gtttccatcg cggttgaaag ttcatgtttc gccttttcgg
     4621 ccgcctcttt caaacgctgg agcgccagcg agtccttcga aatatcgatg ccttcctgtt
     4681 ttttatattc cgcaacaaga tattcctgaa tcttcttatc gaagtcatcg ccgcccaaat
     4741 gcgtatcgcc gccggtcgct ttcacttcga ccgtatcgtc gccgacttcg agcacggaaa
     4801 tatcgaatgt gccgccgccg agatcataca cgacgatctt ctcatttttt tgtttgttca
     4861 agccgtacgc aagcgccgcc gccgtcggtt cattgataat cctccgcact ttgagtcccg
     4921 cgatttcgcc ggcgttcttc gtcgcctgcc gttccgcatc gtcaaaatac gccggcaccg
     4981 tgatgaccgc ttcgtccact ttctcaccga gccgcgcctc ggcatcggct ttcaatttac
     5041 ccaaaatcgc cgcggaaatt tcttcgggag aatatgattt atcgcccatt ttcacctcca
     5101 cgccgcccgt cgctcccgcc tgcacgtcgt acgaaagcca cgccttgtcg cgcgccactt
     5161 ccggatccga aaatttccgg ccgatcaaac gcttcaccga aaaaatagtg ttcttcggat
     5221 tcgtcaccga ctgccgcttc gcaagaagtc ccgtgagccg ttcgcccgat ttcgataccg
     5281 ccacgatgga cggcgtcgtg cggttgcctt ccgcgttttc aaggatcttc ggctcgccgc
     5341 cctccatcac cgccaccgcc gaattcgtcg tcccaagatc gattccaagt attttagccg
     5401 taatgtttat ttaaaattag attaattttc gacgttttta cttatgagtt gaacaacaat
     5461 agcagtagtt ccccttgagt agtaagcatc cgcaactggt aaaataccca aagctgatac
     5521 ttgcctttct ggtaaatcat cttctggttg atgatcacga ggaattatca acggcaagat
     5581 ctttccagat acgaacaaaa ccaacgcgac tattcctaag ataaaattcg gcatttttat
     5641 gttttatctt ctttcccctt gcttactgcc actctcgccg cacgtattat cttatcatag
     5701 agtcgatatc ccgcctcgat ctcttccgcg acggcgcccg gcggctgttc cgattcgact
     5761 tccgccatcg cttccatgac cgccggatcg aacggatcac cggacgtgat gggaattttt
     5821 tcgacgcccc gttgctttaa aagattttcc atctgcgacc ggataaggcg tatccccttt
     5881 tcaatgcctt cggcgcggga atccggcttt gcatcgaaaa gctgttttcc atttatccgc
     5941 aacgcgagat caaaactatc aagcacgcca atcatatccc tgataattcc ttcaatgccg
     6001 tacttcacgg cgtcatcaaa atgcttccgc tcgtcctttt tataattcgc aaaatccgcc
     6061 tgcgcccgct gccagccggc gagatattcg ctcttttcat cattacattt ctgtaattgt
     6121 ccgctataaa actcatttac aatttcccac tcctctttat tttcttcggg aattgaactg
     6181 cagttttgtt tataaaattc gccgaatgtc agtttttttt cttcgtcagc catgatttga
     6241 ttataatctt ttgaatatgc gtagaccttt ccattccttt ccgtcgtccc cgcggaggcg
     6301 gggatcaacg cttccaagtc ttttcttctt gatcgctcat cgctctcttc tccatcgctc
     6361 cttcttaaag cgcgggtcct cgaccaggtc gaagacgacg gaaaaattca caaacttctc
     6421 agcatcctta tcgtcttccg ataatccatc cgcttcggcc caatgacaaa gatcgaaata
     6481 gcggcgccgc ggatctcgta atttccgccg accacggaaa gattctcgct ctttgtcacg
     6541 ggactttttt tgccgataaa aaccttcggt ccgtttccta tcttcttcgc ggcgcggggc
     6601 aggcgttcat ccatcgcttc gaaatcgcga atgacggcgc cgatgccgtt gcgatcttcc
     6661 caatcgaggt tctccacgag ataaggaagg cctgttttat aaaccatatt atcggcgaga
     6721 tccatcgcca ctcccagaag cccgagttcc aacgaaagct tcccgaggaa gtcacgccac
     6781 gcgcattttt cgaaaagctc cttcatgtcg ccgctcctcc gcgaaaacgg cacatccagc
     6841 gcacgctcgg cgaaaaactc gtagccacgg tcggtcggcg atcgtcctgc cgaatgatgc
     6901 agctgttcca gaagcccgtc ttcctcaagc gcttcaagtt cgagccggat catcgcgggc
     6961 tttatgccga aatcgtagtt tttatacaac gccgccgaac tgaccggctc gcccgtatcg
     7021 ataaattcct cgatgaccgc ttcaagaatg cgcgctgttc ggtcgttcat atcggttctt
     7081 attatagtgc cattggcaat catgtcaaga gattgccaat ggcgtttcct ccgtcgtccc
     7141 cgcgcaggcg aagatccacg cttctgtttt tggtgtttgg aattcaaggt ttgacttgcc
     7201 atcgaaaatt ggtacttggc gctttctgtt cgctttccgg aaaaatatcc gaaagtaccc
     7261 atggcaaacg ttcaatcaag tccaatggtc aaaattccga ttgaaatgca aaagcatgga
     7321 tccccgcctc cgcgggaacg acagatgaat ttatcaaagc atctaatatg tcctcatatc
     7381 caccagcagt ccgttccggt ccaaaagcag gacggtgtca tggatcgggt caatgacatt
     7441 gctccccgtc cagacgcgcc actcgttcga caaaaaatcg gcgtcgttca catgtttggc
     7501 gaaacacccg ccataattca ggttatcaag atagctgcgg caggcataat cgttttgcgg
     7561 aatcgaagca ttattcatgt ccggaaacgc gcacgaaccg agagaaagaa tgtattgctg
     7621 gcataggccg gtaaacgacg caatatccga ttggcgggcg taaggacaat ccatcggcaa
     7681 cgccggattg aaaggattca cgttctgcac atagccgatg cattcgttca accggagatt
     7741 gaaatgagaa gtattggaat agatattgag cgtttggccg ttcgaaagaa gaatatcggt
     7801 cggcggggta aggccggaag gatcgtagag gttcaccgcc gtcggaatat attcatcgcc
     7861 gcggtttgat tttatctgcc agcccgtcac atcgatggtc gtcgtggact ggtcggaatc
     7921 ggaaagcgat atttcgccgt acgaataaat atttcccgga gacaacgtgc cgaattttat
     7981 tttatggaaa aagggcgaaa gctgcgccag cgtatatccg gaaggaatat cggcgggatt
     8041 gatggacgac gccggcacgg ttacgctatt gcccgccgaa ggattacctg cgccctcgcc
     8101 gcccgaagcg cgcgtaaaag acggagacgc cgatccgatc gtgcgaacgc cgctttgcaa
     8161 tgtcgtgctt gacagatgcg gcataagcgc ggaaacggat cccgccagtt ttccgaatat
     8221 atgagaactc gcgacataaa gaacgatacc gaacgcaaca acaataaaaa gaataactaa
     8281 tattttttta cccatgtttt ttgccgccgg cgggcgatgg gcggttaacc ctgttgatcg
     8341 gtgacgatgt tcactatttt ttttgcgaca tagacgatcc gttccggttt tttgccgccg
     8401 agcgcggata ttatttttcc gcttgcgagc gaaaggtctt tcgccgccgc ttcggaaatc
     8461 gccgcatcga cattggcaag cgtatctttt actttgccgt ttacttgtac gaccagcgta
     8521 aaagtatctt cttttatcat cgactcgtca tacgacggcc acgcggaaca atggatggat
     8581 gttttattgc cgagtattgc gcgccatatt tcttccgtca tatgcggcgc gaacggcgca
     8641 caaagcttca ggaaaatttc gaaatcttcc cgtgacggcg cgttatcctc gaacgaacga
     8701 agagcaatca taagcgcgct gaccgccgtg ttgtaatgca gctgttcgat atccccggta
     8761 acttttttta ttgtcttatg gatgagcgcc gcaaggttcg cgttttcccc ttttttacct
     8821 ttcccttctt tatttcctcc ttttgtatcc tgcatactga atgctccatt ccgtattccg
     8881 catgcttccc ctaatttcca cacgcggtgt aaaaatcgat cgatccccaa aatgccgccg
     8941 tcgcgaaaat ccccgccctg ctcgaacggc gcaagaaaca taaggtacat ccgcagcgtg
     9001 tcggctccga aatttttaat atacgcgtcg ggattgatca cattcccctt tgatttcgac
     9061 atcttcgccc cgtccttgat aaccagcccg tgcgcccgga attttttgaa cggctcttcg
     9121 aaatcgaaca tctccccgtc gtgcagcgcc atcgcgacga accggacata taatagatgg
     9181 aggacggcgt gctcggcgcc gccgatatac atttccgccg gcagccactt cttcgctctt
     9241 tgcttgtcga acggctcttc gatattttcc gtgcttaaat aacggagata ataccacgcc
     9301 gaatcgagga acgtatccga aacatccgtc tcgcgacgcg ccgcactgcc acatgcgagg
     9361 cacttcgttt cgtgaaaatt ttttacggac gaaagaggcg actgccccga tcccgtcgga
     9421 cgaaaatccc tgacggacgg caatattacg ggaagatcgc tttccggcac cgcataccag
     9481 cccggcatgt ctttattctc gccctttttt gccgctgcac aaattgcgca ataaatcatc
     9541 ggaatcggcg gcccccaata ccgctggcgc gaaatcagcc agtcgcgcaa acggaactgc
     9601 gtcttccgct cgccgccgac aaatttcgta atttcccatt tcgctttttc cgaatccatg
     9661 ccgtcgaatt ttccggaatt cactaaaatg cccgtttccg tataaatcgc attttcttca
     9721 tgcgcgagcc gcgcaagcca aatatccatt tcagcatggg tcatattcgc cgcggtaata
     9781 tcgcccgagt ccgcccaata cgtttcgtgc tcgtccgatt cttccaatgc gatcggcacg
     9841 cgctcatcat ctcccaaatc aaatacgact aatgtcgtat aagaaaccct gttctggtct
     9901 ttatgcgccg caaaatactg agcacgcacc tccccgccaa taacccgtgg atttattata
     9961 tcgttatagc ccgtctcttc tttcacttcc cgccgcgccg cagtgataac atcttcgcct
    10021 tcttcgattc cgcccatagg aaaagtaacc catggatgct ttttccagcg caagcataaa
    10081 accttgccgt ttcgaggatc acggacaaac gcatgaacat tccggcgctc cacgattttt
    10141 ttacccggca cgggcggatt ccgtttatcg atccgctcgg gaataataac ttcccttatc
    10201 ggtaatttat atttcttcgc gaactcaaaa tcccgttcgt cgtgcgccgg caccgccata
    10261 atcgcgcctg tcccatacga gcccaaaacg taatcggcaa cccagaccgg aatatcctcc
    10321 ccgtttgcgg gattgatcgc tttaacgccc ctcagtgcaa cgcccgtttt ttcacggccc
    10381 gcagcgctcc gctcttcgtt gtttttcgtt ttcgctttct cgatatattc ttcgacttct
    10441 tttttgttct gtattttata ttctaaattt tgtattaacg ggtgctccgg cgccagaacc
    10501 agatacgtcg ctccgaacag cgtatcggga cgcgtcgtga aaacttttat cctgtcttcc
    10561 gaatcagaaa tcttgaattc tatttccgcg ccttcgcttt ttccgatcca gtttttctgc
    10621 gcaatcttca ctttttcaga ccaatcaagc gcctcgatat tttttaaaag ccggtcggca
    10681 tactcggtga tcttcaaaaa ccactgctcc aactcccttt tctcgacgac cgtgccgcac
    10741 cgctcgcatt ttccattctc aacctgttcg tcggcaagca ccgtcttgca ggacggacac
    10801 cagttgactg cctgtttttt ccggtacgcc aggccgttcc tccacatctg cacgaaaagc
    10861 cactgggtcc atctataata ttccggatcg tacgtttcca gccgttcgtt ccacgcaaaa
    10921 ccgtttccga tcgactgaag ctgggcgtaa aaacgcttct ccgatatctt cgcctggtcc
    10981 gccggatgca tcccgatctt cagtgcgtaa ttttctgaat gaatgccgaa accatccaag
    11041 ccgatcggct cgaacacgtc atagccctgc atccgcttaa ggcgtccgta aatatcgctt
    11101 cccgtgaacg catacatgtt ccccacgtgc agcccttcgg cggacggata cggaaacatc
    11161 atcaggttga aaaacggctt ctttgccgca tccaaatccg gctcatagat tttctcctca
    11221 agccacgcct tctgccattt tttttcgatg ctgcccggat cgtatttcga cattatggct
    11281 ttattataaa acgatgagag gtttttatca aatttaataa tgccgaagaa aatatattcc
    11341 gttgcccttc cgaagaggaa ggttaatgct tcccattccg aaatcctcaa agcgtaaatc
    11401 ctcgccctgt ggcgcaggcg aagccgccac ttcatagagc gaggacgaca gaggaaataa
    11461 taaatcaata ctttttcaaa tcccgaaaat ccgctttgcg tttttcgcga tggcgtccgc
    11521 cattgcttcc gtggaaatat ttttcagcgc cgcaagtttt ttcaccacct cgataacata
    11581 caacggttcg ttacgtttgc cgcgatacgg cacgggcgcg acatacggcg catcggtttc
    11641 ggacaatatc cggtcaagcg ggatcatttt aattatttcg tcgtaatcgc cggtattttt
    11701 tcctttccgc ggcggaaaag tgatcacgcc gccgaacgtg aacgaaaatc cgaacgccaa
    11761 aagttttttc gcatcctcag cggtaccgga aaaaaaatgg atcacgcccg gagaagcggc
    11821 aagagatccg tcatcgccca tgcttttgcc ggcagataag cggcgagaag caaggagctc
    11881 tatcaaatcg gaaaacgcat cacggcagtg gatcatcagc ggctttttga cctcgcgcgc
    11941 aaggtcgatc tgcgcgatga atacttcttt ttgtttttgg atctgcaatt ccctgctttc
    12001 cgattcgtta aaatggaagt aatcaaggcc gcattcgccg atcgcaacgg tcttcggatg
    12061 catggcaagc gcacggtaat aatcatggtc gaaaatttct ccacgggaaa cggatgcgtg
    12121 tgccttttcg ccgccgccaa gctcatccgt atcgtgaaaa gattcgcttg tgtgcgcggg
    12181 atgaagcccg acggcggcgt atacgccgtc ttcatattct tccgcgacct ccacggcgcg
    12241 gcgcgacgta tcgcgttccg tcccgacgtt catcatccgc acgccgcctt gcagcgcgcg
    12301 cacgatgact tctttgcggt cggcgtcata cgccgcaaac tgcgcgtgcg tatgggcatc
    12361 gagaaaagga aatgatggca tcgtaaaaat tattcgctat ctatgctttt tctgttatcg
    12421 ccgcttcatc caatttttat ttttcggcgc ccgcgcccct ctgttgtcat tagcttggct
    12481 gaggacaata aaaaaacaaa gacatcactt tatccgcgga aaaagcgttt ctattttttt
    12541 tgcgcggaga acattccctt cccgccggat tgcgccggtt atcttcgccg acgtttcggg
    12601 aaggaacggc aagaggacgg ttgcgatacg ttcgagcagc atgacaagat cggatagcat
    12661 ccggcggcgg gcggcgtcat ccgataccgc ccaaaccttt ttctcgttta taaaatgatc
    12721 gccgaatgaa atcgcgcgcc ataccgccgc gagcgtctcg ttaaatttga atccgctcat
    12781 gctctttatc acgccggcat gcatggcggc aaccgcctgc tcaaacgcac cgtcaaacgg
    12841 atcgcatgcc aatgtttctt tttcggcaag cgtcagaacg cgggcggcga agttaccgag
    12901 gccgttcgca agatcggcat tataccgctc tttcattttt tcttccgaga aatcgccgtc
    12961 ctggccgtac ggaacttcgc gcaagaggaa ataccgcacg gcatcaacgc cgtagcgcgc
    13021 ggaaagttca agcggatcga tcgtattgcc gagcgacttc gagattttga cgccgttcgc
    13081 ggtgagaaat ccattcgcga taatctgccc gggcagcggc aatcccgccg ccataagcat
    13141 cgccggccag atcacggcat gaaaacggag gatgtccttg ccgaccgcgt gaacatcggc
    13201 aggatgttct tcccatcctt cgatgccgac cgaagaaata taattcacaa gcgcgtcggc
    13261 ccacacataa atgacctgat gcggatcctg cggcaccgga atcccccagt cgcgctgctc
    13321 gcgcgaaaaa ctgatatcct caagtcccgc ttcgacgaaa cttttcactt cctgaaaacg
    13381 attcgcgggc gcgacgaaat cctggttctc gttataaaaa tccaaaagcg gcttttggta
    13441 tttcgagagc cggaagaaat aattctgttc ggaaacgaat tcgggttcgg tcttgtgatc
    13501 gggacaaagg ccgtcaatca gttcgtcctc tttcatgaac gcctcatgcc ctttgcaata
    13561 aagcccttcg tatttcccga gataaatatc tcccgccgca tcaagccgtt cccaaaaatc
    13621 cccgacggtc gcgctatgcc gcgcttccgt cgtccggata aaatcggtat tcgatatgcg
    13681 aagccgttcc catgccgctt cccatattcc ggccatccgg tcggtatagg cggtcagttc
    13741 ctcgccgttt tttttcgccg catcaaccgt tttctgcgaa tgttcatcga cccccgtcaa
    13801 aaaaagaaca tcttcaccct gttgccgccg ccagcgcgca atcgtatccg caatgatcgt
    13861 cgtatacgcg ctcccgatgt gcggcttgtc gttgatataa tagacgggcg tcgtaatgta
    13921 aaattttcct ttgttcataa tgtaatgttc ttacttttgg cagcgagacc gcagggaaaa
    13981 tttactttcg ttgtgtgcga gcaaaccaaa taaaaaaatg ttgcgtatcg tggtattttt
    14041 tacgcttctt tatgcgccag gcgtttttga tacgaccaat cctcgacgac ttcctggaac
    14101 acggcggctg ccggaatggc gatcacgact cccaaaaaac ctcccacctc tacgccaatc
    14161 agaacggcga cgatcacgat cacgggatgc aatcccaccg aacggcggtt caagagcggc
    14221 accaggacat gcgcctcaaa ctggtggatg atcaggaaaa agataagcgc gtaaatcgca
    14281 agcgggaccc ccaccgtaaa tgccgaaacg acgccgagcg cgccggcaag caacggcccg
    14341 acaaaaggaa taatctcgaa aaaagcgcca agtaatccga taagaaaagc gtacggaacg
    14401 tgcaaaacgg tcagtcctcc ccagatgagc aatcccataa tcacgcttaa aaatatctgc
    14461 atatggaacc acgctcccat ctttttccgc gaacgctcgt aaatacgcag cgtcgacact
    14521 tcataatccg gaggcgtcac cactttcagg aaccgttcga cgccgtcgcg gctcaggcat
    14581 aaataaaaag aactgacaat caccgcaagc gcgagcccga cgccgcccaa agcttgcgaa
    14641 aagaaaccaa agggcgatgc gccgctggaa aagatctgcg aagaaagctg gctcacgaaa
    14701 ccgcgcgccg tgtccacgcc gttaaagccc atgaggaaac ccagcccgct gtccgttttc
    14761 tcgagcgccc ggagcgccat gttcaggtcg atgatggtaa gcggcagcac cgtataaacc
    14821 agaatgacga ttgccaaaac cgccgccaaa aaaataatga tgacgctgat gctgcgcggc
    14881 aaaccgatcc gttccagctg gctcactacg ccttcaagcc ccgatgaaat gacgatggca
    14941 agaaaaagcc cgagcagaat ttcgcgcccc aaaaacaaaa ccaccacaaa ggaaacgaaa
    15001 ataaatatcc gccaaaggct gccccatgaa atttctaatg attgtttcat agatgcaaaa
    15061 tagaattttc tcctccgtcg tcctcgcg
//
LOCUS       DAIDDT010000014        15013 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_14_length_15013_cov_6.660686, whole genome shotgun sequence.
ACCESSION   DAIDDT010000014 DAIDDT010000000
VERSION     DAIDDT010000014.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15013)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15013)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15013
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14_length_15013_cov_6.660686"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3988 a         3824 c         3671 g         3530 t
ORIGIN      
        1 gttaataatt atttaatacg tccttgctat ccctttgtca tcctcgcgaa atcaaagatg
       61 atagcttact ctgttactac gatatacccc accgggcctt tcgccgaact gccgcaggaa
      121 acgcaataaa aagtaaactt gccggacata gtcgcctgaa actgaatttt ctgcgtcttt
      181 cctttcgtaa gcaccaccgc aagaccgtaa tctggctggg tgaaatcgta atttttatct
      241 accgccgtta tttcgaggtc gaccgtatcc ccttttttca cgataacggt agtaggaatg
      301 aactcattat tatccgcact gatagaaaat tgcctataac tcgcgttgct tgatggagcg
      361 gcggtgcctt gcacctgtgg gacggcaaca ttttgaggaa cgttcttatc tccttcgttc
      421 ggcacgacaa tattcgcggg aaccggcgcg ctggttgccg attgcggaac cgaaccgctt
      481 gccgcggccg gcgcggcgga accggaattc ttcggcggcg gattattcgc gccgggccaa
      541 aacagaagaa caaacagaag aacgataacg atcgctcctc cgatgatgac tattgttatt
      601 ttatgttttt cccacatagc gttgataata ccacatgaaa tctgtctcaa aataggccgg
      661 cggatttttt gtaaaatcta tttttgaggc gggttcttgt tacggacacg ttaccgtggt
      721 ggtcgcaaga gcgccgcttg ttatctgaac ccgataacag gtccccgtac tcgtatcctt
      781 caggatcagc ccatacgacg ccgaagaaat atctatatct cctgacgcaa ctgttaatgc
      841 cgagcctggc gcggtggtgc cgattccgac attgccggag gaattaatta tcatcgaaac
      901 aggattattc catgtcccgc caacaaactc aatgctctta ctggaagcgt taggtgaaat
      961 aatcaaatta cctacaccat tcgttatcaa tacatctccc gcgaccgcat ttgctaagct
     1021 gcctgcaaat ggcatgatcg taaaatcgcc acctcccgtc gcgtcgttct gaatattcaa
     1081 agcctgataa taggaactat ccgatctatg aatatccagc gccgcccccg gactcgtcgt
     1141 cccaatcccc acatcgcccg ttgatgaact gatgaaaagc gtcgagctcg cggaaagcgc
     1201 cgtgcccgcc gcattccagt aaggaacata aaatgccgtg ccggtcgcgg aaacccccca
     1261 tggaaggccg ttccaataca gattaccgcc gacgttatag agccgatccg cggtcgtagc
     1321 gggagcggaa gatggctcga agtaaagccg cccgttgagg tcgagcgctt cctgggggct
     1381 tgcggtgccg ataccgacgt tcgtgccgct gaaataaata ctctggttga cggaattcca
     1441 tatgttgccg gcgacgccaa gattataggt cgccgttgac gacggaagaa tattcgaatc
     1501 gaacgtcgcc gccgaaacgc gcgcgatgga ggtctggagc atctgggaaa acgcctgctc
     1561 gatattcgac gtgttgtacg cgatcgtgaa agcgacgctc accgaatcgt gcgacggcgc
     1621 gttcgcgcgc ttcgtgaacg tgagatcgga aacggtcacg cggttcgacg tgagcgcctg
     1681 cggcgtcccc gtcgccgtct gctgaagata gacggttccg ttctgcaatg aaatataagt
     1741 cggatcctgc gcatccacgc ccgtataaag cttgagcgtc gaagtcggcg tatcctgcgg
     1801 tatgtcgatc aagctcgatt gctcgacgta atactgtatc ttctggagca aggcctggct
     1861 ttgcgttccc acctcaaccg ccgacgattg ctgcacttgc acgcgcgtca cggcgatcag
     1921 gatggtgatg aatgcgacgc tgatgaacgc aaaaataccg agagaaataa gaagctcgac
     1981 gagggtgaac cccatatgaa ataccgaccg atgagaacgt agagttttat gatgacgaat
     2041 actcattttt ttattttctt atattccaaa aaaggatcgc atttctgacg gggcggatgc
     2101 ttttcgattc gtacgtataa ttttattata gcacacgctg aagaacaagt tttcaatatt
     2161 atgggaaaaa cctccttttc acgctccttg tcattgtccg tgaagacgga cgacccatgc
     2221 ttcccgtctt ttcttttgat ttttaaagca tagattctca gccaaactga gaatgacaga
     2281 cgggattgtc atcgtctgcg attgatgacg agagctacgg ataagagtta aatataccct
     2341 gctatttctt gaccctgtct atttccttcc ttcttttcgg cgttacccag aaaccgagcc
     2401 gatattttcc gccgagcatg aggcgcgttt gcgcttcgat ggcgggaatg gcgccgaacg
     2461 cgacaagcgt gaccggcacc aaaagccact gcaaaaaata aacgccgtaa tccccaatcc
     2521 tcaatccgcg cggcgcgggc ggtaggacca aaaagctcag aatcgccgaa ctcgcgatgc
     2581 cgaccatgga aagttcgatg ataaagcggg taatatgcgg caagttatag gaaagaagcg
     2641 tttcgttgaa atgatggccg ccgaaaaata gcggcaacca gccgagtgcg aagatcataa
     2701 gcgcattggt cgcccacgaa tggtatcctt cgatggcatt aaacgtccaa taccaccgtt
     2761 tctttttcgg gatcgttttg tccgcgcgga acccctctaa tatgtaagga acattctcgg
     2821 atccccacgc ccagcgccgc tgctgccggt aaatattatt taacgtcccc cagaacgttt
     2881 tcgccgccgg tgcatccatg gaaaccggaa aaaagagcgg cacgacgcgg aaatcgccgt
     2941 ggtacttgaa aaagccctgc caaaatatcc gggaatcctc ggacacgatg tctttttgcc
     3001 agaagccgat ctcggaaagc gtcgcgagcg gcatggattg cgtggaaaac gaggttaatc
     3061 gctccggccg cgactgctgc atcatctgcc aaaaggaaca ggaaaacgaa accacgcgcg
     3121 cgaacgcgcc gctttcgtaa atgttgttcg taaaaagcgg aatcggctga taaatggagc
     3181 gtgacggatt ttccgccgtc aagaaaacat atgtgagacg gccgaaatat tcggggaaaa
     3241 tctgcgtatc gatatcgaaa accgaaacca aaacattgcg ctcgtcgatc ttcataggat
     3301 caatgatgat ttctttcgcg cgcgcggcgg cccacgcttc gttcgatcct tttccgggaa
     3361 tctcgccccg aagatctttc ggatgcaccg taacaagaaa cgccccgaaa gccgcggcga
     3421 attctttttt tattttttct cccgtttccc gcgccgtctc ccccccccgt tcttcgagcg
     3481 caagcacgac cgcgattttt tccatgggat aatgcgcgcg cgccagacgg ataaaacttt
     3541 cgcggacgac ctcgtacggc tcgttataca tcggcaaaat gacgagatgc tgcacgccgc
     3601 gccagacgga aaacgagtcg gctaccgaaa acgctggatc gaaaagtttc ttttcccccg
     3661 ctgcttccgt ttttcgtttt tccaattccc ctatccaatc gattttcatg ttctttttca
     3721 tttccgcgaa cgtcgcgttc agatgaaacg aaaggtagat cgttttcaaa agccagtaga
     3781 tatcaaaaag gataatgaaa aacgaaaccc ataccggcag gagccatgaa aataaaaaca
     3841 tcaaaacaag cgtaagccac gccaaaattc ccgggatcat ttcatagaaa cggcgcatgg
     3901 tgataaatag aatatagaat ataaagcata gaccacggaa tttaggaaat acctcctccc
     3961 tttcgatctt cctgctctat tctcaatgct atattcttct tttttattgg tctttgaaaa
     4021 aaatcggtct ttcctatatt atatcatcac gctcgctctg tcatcgtttt ccgcgcggcc
     4081 tcatcgtcta atggttagga cggcgccctt tcacggcgta aatcggggtt cgattccccg
     4141 tggggctacc agattccttt gaatttactg tgttgttgca tgacttttcg aattgccgaa
     4201 aagtcttata tgtttctgaa cttatacata ctgcgaggct gattaatgcc ccgcgcggcg
     4261 gccgcggatc actgaaccgc gatccgcggc gcaacccgat aaaactgata aaaatacgat
     4321 acgccatccc ctgccggaca ataacgcaat acccccgaca aaatccgttt cgattcgatc
     4381 gctttcagct gataagcgcg gatcgaaacc ggataatgat cgggggtatt gttggttatt
     4441 gcttttgcag gcaattatgg caactgcgga agcggaagat cgagcggtgg gcacataagc
     4501 tcattgatcc acctcttcgt cgtcttatag acgtagcccg taggggtcgt ttcattcgcc
     4561 aatccataag gaatccacgg caagagaacg gtttctttat acttcacctg gaattgttcc
     4621 actgcatgat acgtgagcct tccataataa cccgttatcg gaagattggt cccgagattc
     4681 tgattgagga ataattgaag cttcttcacc tgagttgggt cgttatcggc tcccaatctg
     4741 atataggaat caagataaac gccgcaactt atagaagctc caagaacctt accgatgcgg
     4801 ccttgaataa gatcaatcga cgcgttcgcg gtccccattg tggtacccgt gacggcacgg
     4861 tactgcacgt agaccgtttt ctggcctgaa ccagaggtaa taatccacgg caatgtcgtc
     4921 gaatacggta tccatgtcga actcgaaaaa tcggaagtgt tcgaaacttc cattgtgtac
     4981 gcttccgttc cgtaaagcga aagcataacg ctggtcgtcg cggtcgatga cgcgccgccg
     5041 ttgatcgtta aatcatacgc cgagccgcca cctgacacta tcacgctacc gccgcaatta
     5101 ccgctgcagc ctggagaagg gttaaccggc tgaggcgtcg gcgcactcga cgtcgcaacc
     5161 gtgatggtcg cacttgcact cggatcaggc gtcccattga cgtccaccgt attcacgata
     5221 gtcgtgccgg aagacgcatt aacaagcgcg gtgacaatca tcgtagcggt agaccccacc
     5281 gccatattcc ctaccagcca tgttcccgtc gaagacgcat aactgccttg cgaggtagtt
     5341 gcgctgacaa aaacaagacc cgagggaaac gtattggtat cggttgccac aacattcgtc
     5401 gagctcgccg gaccgggatc tgaaattaca atcgtgtaat gaaccgtatt cccttgataa
     5461 ggagtggcat tgtcaacgat cttcgtaacg gcaagattgg cggagggcgt tgaagtcgtg
     5521 ttaccaccac aattactcgt gcaagctgga gtcgccacta tcaaagaagc ggctgccgca
     5581 ttgttgccca tatcgggatc ggtcgatgac gccgattcgt cgacggtcgc cgtattggtg
     5641 atcgttccgg atgacgcgtt cacgatggcc gcgatcgtaa gggtcgcggt tgagcttgcg
     5701 ctcatgtcgc cgatcgtcca tgtccccgtc gagctggcat aagagccgcg cgatgtcgtt
     5761 gctgaaacga acgtaagtcc cgccggcaaa atatccgtcg ccatgacacc ggtcgaagtc
     5821 gcaggcccga gggccgaaac catcaccgtg taatccaccg tggcgtcttg atcgggattt
     5881 gtgttattga cggttttcgt aatggagagg tcggcccgag gagtcgatga cggcgtggaa
     5941 gttgaagtac tggttgaagt actggttgaa gtactggcac cggtaaagat gctcgtaccg
     6001 acactcgcac tcgcgaacgc gctggaactc gcggaacttg tcgcgtcaag agtactggac
     6061 gaacttgaag tcgtcgtaat ctgcgcgctt gaagtcgcga tcgacgtcgc actggtaatg
     6121 accgaaccgg tcacgtcgat aatagtagga ttcgtcgaac tgctgccggt gttaaccacc
     6181 tggacgcttg atgtcgcagt tcccgaagca gtggaacttg ccgagccgtc cgcggtggcc
     6241 tgatcagaag aactgcctcc ggcgatgctc gcggaactcg aagtgtttga ggaaacactc
     6301 gaagtggcgg cgttggtcgt gtcaatctga gtactgctgc tcgtagtact cacatagaca
     6361 taatcggtcg ccgatgccgt agagctcgaa ttcgaagaat cacttgctgt gacctgatct
     6421 gaatgcacga tagaaacagc gaataatcca tttcccccga ctaaggaacc tattaaggcg
     6481 cattggatcg tgacgatccc aataaccgcc aaaaaccggc ttctcttcag ggaagaaaac
     6541 tttctggcaa actttttcat ctgttttttc tgtattattg atttttccga ctcttttagg
     6601 tgttaaaatt taaaagagcc cgaaaaatat attgtcaaag agctggggaa aaccacaaaa
     6661 caactgttat atagcttaca atacgaacag ttatttgtaa agcgatatct gttgacaaaa
     6721 taatataacc atgtatactg ttgtcagttg atttgacaat aaaaaaataa aaaagggaag
     6781 aggaacgcgc atccgcggta cgacatcatg cttccattta acatttaaac ggctcccgga
     6841 taatcttccg gcggtcgaaa ggaggtttta tgaagaaaat tttcttttta ttgcttgcga
     6901 taacattggc gttatcgttt gcagtagtgc cacccgcggc aaaatcgacg tttgccgatg
     6961 tcacggtaac ggcgagcccg tcgtcgtcat cgacgtcgac gtcatcgggc accggcacag
     7021 cgacggcgaa tccgacggcg acggcaccaa cgagcgtcac ggtgaagaat cccgtgacgg
     7081 tgtcgtttcc aacgacaccg ctgaatgtca cgccagcgac ggcggaaggg atacattgct
     7141 tcgtcttcac gcccgcaaga atgacggtcg ccgaggcctg cggtaaagcc ggagagatgc
     7201 cgaatggcga cggcagagga atgttgacta ttctctacca agggaaggac aacattgccg
     7261 cggcaatggg aggaacaatc ccatttggcg acttcgttga acaaatcgaa gtcaacggca
     7321 aagggaatat gtacatgcat cccttgttaa aggcaatctg cgcgcttgcg aatcacggcg
     7381 caaagactgt gatcgttgat ttcacttcgt catccatgac ggagacagca gcggcgggga
     7441 tcggcatggg aacaggaaca aatgtttcct tgcttggaat aacgatagga aagacgtgga
     7501 caatggcaaa aacgttgttg cacgtcatag cattgggttt tagcaatgct atcggctcca
     7561 aggcgtctaa ataatctttt tattttacac taccccgaag acaattcgtc ttcggggtat
     7621 ttttttgcga cgaaactgaa gaaatgaaaa aacgtatgaa aaatgacggc tgttccggct
     7681 acaaagacga acagtggcgc ttcccgacta ttcagcaaga aagaggaagt tcatcccctc
     7741 tgtcgtcctc ctgaagagga ggatctacgc tttccatctt aaattcggaa agcgtggatc
     7801 ttcgccggag tttatcccga gttttcgagg agcgaggacg acggaaagag cacgcgagag
     7861 gttaccgacg agccccttta caaaatttta gaagcgtgta taatattgac acttgccgat
     7921 aaaacgacga gcgctctttg tcaaataaaa acggcctaaa agccgtcaat ataaggagaa
     7981 gaataataac gaaagaaatg aaaggccgat ggacgggcgt ccttgccggc aaataaattt
     8041 aattaacgaa agaaatgaaa ggaggaaaaa gaaatgaaag gaaaatttat ttttttggtg
     8101 attgctatgt ttatcgcctt caccgtaccg gcaacatctg cgaatgcggc cgataacggc
     8161 tcacaaacga cgcaagcggc cgcattgcaa aacgcggcgt ttgccaacgc gaaaaccgcg
     8221 gcgcaactga acgcgaccgc aacggcgttg aaaaaagctg cggcgcggca aaaagctgcg
     8281 gcgaacgaca acgcaaaaac agctacaaac ctgagcaggg ttacgacaac gcaaaaagtt
     8341 gtcgctgcaa aactggttgg aacgtcgaaa acgctcgcca atgccaccgg cgcgaacgca
     8401 aagacggcgg cgcaactgaa cgcgaccgca acggcgttga aaaaagctgc ggcggatcaa
     8461 aatgcgaccg caacggcaaa cgcgaaattg gtcgcaacca tcaagatgat tgtcttgatt
     8521 gcggccgcga tcatcgcgac ggccgcaata actctgatcg ggctaatgat caggggcaca
     8581 agaaagaaaa ataagcaaca ggaagagacc gcgaaaacgc cggcgatggc aaaccgcgaa
     8641 atacttatcg accctacggc ggaagagttg aaaaggatcg ccgtcggaag aggtgagact
     8701 gaaataaaat tcaatcttcc gctgaaagaa aaaagtttta gctgtatcgc ccatattgcg
     8761 gacggcgaaa tctacgtgag ttttttaggt gatcctacaa gaactctcgt gggatgggat
     8821 acgagaagat cccatgcggc gaagctgata aaaaaagcaa gcgaactcgc cgcttaaaaa
     8881 taggccgggc caccggcgga atataatttc aaaaaacatc tcaattcaat aattaatttt
     8941 aatttgaacc aaagatgtct ttatgaaacg tgtaatccgc aaagtggttc gttgcccgtc
     9001 aaaagggacg ttcgcatgga gcgaagaaaa gccgataatt tgttttaatc gtcgaggttc
     9061 tctactcccg attgttcggg aaaggaggaa taatgaaaac gtacgaaaag gcgataataa
     9121 ccctgctcgg gattattgcc gcatcactgt tcgtcatcgc gggctgtctt ttttatcttg
     9181 cgttacattt aagcgcatac gcgccaaatg caatgcagaa aacaacggca ccgacgacga
     9241 cagcgaccgc gtgcgtaccg tgttgtccgc ctgcggtaac gaaggcgata aaaccggtca
     9301 agtatgcgac gcgtcctgcg aaaaaaataa gacgggttgt gataaagcat tggaaacccg
     9361 tcgcgtacta ccgtcattac gttaagccga taaggcaaaa cgtgataaaa gcagtgacga
     9421 aaccgaagat tgcaataaaa acttcggtga cgaaaccgca agcggtagtg atagcagtaa
     9481 agaaaaaacc ggcaataaag gcaaaagcaa agtcggcaat aaaaaaagaa acagcgacga
     9541 cgacgcagag tgagtggaat gattgcgcgg aaccgaaagt gttcccgcaa cgatcatgta
     9601 aactcacgcc acaacaagag cattacattg ttgcggagtc gaaggggaag attaccataa
     9661 cgcagttaca aaaaaaccgc gcaatgaata tgtctttctg tgaaattttg aaatcgagtt
     9721 cttcttctcc ttaagctaac gcaatttagc accacatccg gacccttttt aaaaataaaa
     9781 agggtccgtt ttttattcca aaaaagaacc gcggcaatgt gcaaacaaaa aaagaaaaac
     9841 atctctcctt ttgcgggaac agcaaaaaaa gcgcaagcgg cgcggcgcgt ggattatttc
     9901 aatatgttat accgcggtac cccgtcgtat aagcatcaaa aaaatcatca aatcgaatct
     9961 ttataaaacc gtgattgccc tcgaaaaaag atactggttc agataaatga aggacgcggc
    10021 ggcaataacc acgaccatgc cgcccatgat gagctgcgga tgctggatgc ggatctttat
    10081 gaaaatattg aggctgagcg cgagcaggaa aaggacaagg atgccgaata ggagatattc
    10141 aacggcaccg tacggccccg tcagcaatcc ctgccagaac gacgactgcg gtgcgcgcaa
    10201 cgtgttttgc gacgcctcgc tcgtacccaa aacggcgggc gccgatctta tggcggcaac
    10261 cgcgatgaaa tcctgcaccg acgtcgtggt ttccgaaatg ttcgatgccg tcacgaccac
    10321 gggcgctatt tttacaggag tcgctttagg cagcgacgcg gaagcgacga aaggcgcgct
    10381 ttttttcggc gcggacgccg gtacattttt cgacacgggt accgcggatg cggaaacgac
    10441 ggacgcatca ggcgtgttct ccgcaagcgg cagcgcgaga gccggcgtac cgaaaagctc
    10501 tacaacgtat atcgcgggat ggccttcata ctccccgtcc gccgttgcaa taccgatctg
    10561 agtgaaattg ccgttcaaaa tgtttgcgcg atgttccgga gaattcatcc atgcagtcgt
    10621 cacatcctgc gaatcggaaa aatcgaccgc gagattctcg ccggcatacg aaaaatcata
    10681 gccgacgctt tcgaaccaat accacggcgt aatgccgtcg ggactcgtat gcgcgaaata
    10741 ccccttggaa gccatatcgt ccgctttgct ttgcgcagcg gtccgaagaa gcggactcac
    10801 ggtaagcccc ggaaggttat tgtcgatacg gttctggttc gtctcatcca ccaatacatt
    10861 cgaaatgatg acaccggaaa gctgcgggtg cggcgccgcg aaacgcaagc cggcaagaaa
    10921 gacgccttca gaaacgaaaa gtacaataaa aaagaaaacg acggcatgcg tgcgcaggat
    10981 atggggccga tggtcgttcc actcgtgcgg aataaaatat tccttcaaaa atacaattat
    11041 cttttgcaca taattattgt gccataaatt aaagatttac gcaataattc attaatattt
    11101 gaaggggtaa accagtgtca aaaatcacgg attttgggta agatgggttc taagggatcg
    11161 gtacagagat accaagattc agttctgtta aatttagcgt gatgatccat tctccttctc
    11221 ctacagccag gaaagtgtca atcggccttc tcgcagccgc attggtcgtt ttttttgcca
    11281 tgatggcatg gcaatccgtt gcgccgcaac aggacacggg ttctttgcca ataaagcagc
    11341 ctccatctat ccttcccgaa gcgacctcgc catcgtcgcg ccgctatctg aaagtgccaa
    11401 tcctgatcta tcacagcatc gcgccctata aggcggatat gtcgccgctt gtcagggaat
    11461 acaccgttcc tcccgaatcc ttggccgaac agcttcagta cttgcagcag aacggatata
    11521 ccgttatctc tttcgaatcg cttgccgatg caatggtcga tgcgagcgca acgctcccgt
    11581 cgaaatccgt cgtgctcacc ttcgacgacg gatgggaaga ccaatatcgc gatgcattcc
    11641 cgctccttaa aaaatacggc gatactgcca ccttttttgt cttcacgaac ggcatggata
    11701 ccacggactt catgacatgg gaccaactga aagaaatgca ggccgccggg atggatatag
    11761 agagccattc cgtgtcgcat ccctaccttt ccgccatcac cgataaagcc gaactttgga
    11821 aggaaatcga ggggagccgg gaaatcattg aatctcattt aggccgtgcg cctgacctct
    11881 tcgcttatcc tttcggcgat tacaatgccc ttgacatcgc gatgctcaaa gcagccggtt
    11941 accgtgcggc gcgcaccgat ctcggatggg cgctcccgac tgccaccgca aacctcgcga
    12001 cgaccacaga cgcactttac cggcttccag gtgcccaggc aacggatgat ttcgaaagat
    12061 ttttgtcgtt aataaataaa tgaatagcgg cctctgaatc ccgggctgaa attgtaacca
    12121 cgacggaaaa tcgaaccttt tccgtggaag aagatcgtcg aaaagaaaaa tgccgtagaa
    12181 cacgaagttg cgatggttcg aacctccgaa aaatcaatca ctgccaatca aagattggat
    12241 ttgtcccgaa aatttctaat actgcgggag ctggaggaat cggacctccg atcgttgttt
    12301 tggagacaac cggtatacca cttacctaag ctcccgaggc attattatac taatggaaaa
    12361 atataaaaaa caaaacaggt cttttgatat tatcacaaaa agcggactta aaaatcccgc
    12421 gcgcgggatt tttatgaaca ctctgcttct ccattaatta ataatttaat ctcccctatt
    12481 ttcccgtgat cttcgcctct ttgtgcggca atgtcttgcg gcaccaatcg cagaattttt
    12541 tgagctctat cttcctttcg accgacttct tatttttatg cgtataataa ttccgccgct
    12601 tgcagacgga gcacttcaat actattaaac tgtccgagcg ttttgccttt gccatgaaaa
    12661 cgatagtact ataagacccg cgaaatagca aatttttctg aaaacaacag cactttaatc
    12721 tctgattgtt gagccacctc ccggatttga accggggacc ttcgctttac gaaagcgctg
    12781 ctctaccaac tgagctaagg tggcgtttct gcttcctaag aatttatcag gtttttttta
    12841 tttttacaat gcgatgctga cgttatcccg aagcttcggg ataacgaaaa tatccggaac
    12901 caatgctctt ttttaaaaaa ggcggcgaga gatacccgcc tttgaaagta cgcactacaa
    12961 cttcttgttt ttgccaaggt tccgcaaaaa atcattctat aaaaatacct ctctccgaga
    13021 gattgataat ttccccatcc tccactcatc cactcgtcac tcctcctcaa tgccgttgaa
    13081 cagttttatc ctgtccgtaa cgatcttctg tatttcaaaa accgccgccg gaaggatttt
    13141 atacggcgcg atttcttcgg gattattttt caatcccgct tccacgccgc ggcgccaggc
    13201 aaggcggagc tcgctcgaaa catggatgat cgtcatgccg gatttcgccg ccgcgacgaa
    13261 atcttcgtcg gccgtgcccg atccgccgtg cagcgtcata tattttccgc acgccgccct
    13321 gatcgctttg atgcgttcga tatcgagccg cttatgttcg gcgccggtca ccattgtttt
    13381 taaaaggccg tgcatattcc ccactgcagg cgcaagaaca tcgataccgg tagcctgcac
    13441 gaaccggacc gcttcttccg gcgtggaaag cgcgatatta tccggccgct tttccatcac
    13501 ttccgacgat gtgccaatat aaccgatctc gccttcgacg acgatttccg gatcaatcgc
    13561 gtgcgcgatg cgcaccgcct ctttcgtctg cgcgatattt tcttcgatcg gcagtcccga
    13621 accgtcaaaa agaatttcat cgaaacctgc cccggcggcg gccttcactc tctccaatga
    13681 atgcgtatgg tcggcgttca ggaaaatcgg ataatcatat tcttcccgca tgctccggat
    13741 aagcgccgcc gcccggcgca cgccgataaa atcccgctct ccttccgaaa cgccgatgag
    13801 gataggcgtg ctgtcgtgcc gcagtgccgt aatattttgg ccggcggcga aaattccgtt
    13861 gagtgcggca agttcggaaa tattaaaatg gcctatggca acccgccgcg catccgcatc
    13921 cgccaaagat tcacgtaatg ttttcattga tatgatatgg tgattacccc cctttttttt
    13981 gaattgctcc ttatatcggt ttcggtttat aatttttcgg cgcttttttg agcagcgaaa
    14041 gcagtgcctg ttttttcaaa agtcccgtct gtgctccgaa gtattggacg acgtgcattg
    14101 attcgatcgg cgcccagcgg agcgcgtcat agatcggaaa gccgtagatc aaagcgcaaa
    14161 gaaaaccggt cgaaaaagcg tcacccgcgc ccgtgcgcga aataggcggc cttggatccg
    14221 gatacggcgg catgaaaaaa cgatggccgc cgtccgaagc gtacgctcct ttcggtccgt
    14281 ccgtgatgac cacgatcttc ggcccgagtt tgtgtaattt ttcaaacagc ggcacgatgt
    14341 ccgatttttt gcttcccgtc accagttccg cttcctcgcg attcacaaaa agaacatgcg
    14401 tcagacgata gatctctttt aatttttccg gtccgaactg cagctggaac gtgccgggat
    14461 taaaacccat tttcacgccg ggatgttttt tcacgaaatc ggcaactttc ttatgaaacg
    14521 gcagagtatg ttcgccgatg gacgaaaaat agagccaatc ggcatgacgg tcgatgtcgg
    14581 ccggctcgaa ataatcgtaa tcgttgtgct taatgaggat tgtccgctcg gccttgaaat
    14641 tcaatacaaa atgatagttc gtcggctttt gtttgttcaa tttcacgaac ttcgtagcca
    14701 cgccctcttt ccggaaaaca ccgatgatct ccttcccgta atgatcgccg ccaaccgcgg
    14761 taagaaatgc tgtctgcaga ccgagacggc tcgcacctac ggcgacattc gcggcattac
    14821 cgacgccggg aactatcgtg accgattcgt acggaatctt gtccgcgaac ggcatgcata
    14881 attgggggtg ttcacggtcg gcgccgaaac gcaccgacgc cacctgtagc gttacaaagc
    14941 aatcggtcgt gcaatcgccg atgcaaacaa gatcgtattt tgatttttgc ggcatgtttg
    15001 aattgtgcag gat
//
LOCUS       DAIDDT010000015        14896 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_15_length_14896_cov_6.318510, whole genome shotgun sequence.
ACCESSION   DAIDDT010000015 DAIDDT010000000
VERSION     DAIDDT010000015.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14896)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14896)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14896
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15_length_14896_cov_6.318510"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3799 a         3870 c         3324 g         3903 t
ORIGIN      
        1 atagtaaaaa cgacctttgg tttatttacg gtacacaact catcgtttat agtgtatcgt
       61 aaataaggga tttttcaaat tccggcgccc tgcttccggt tccctgccga aaaaatatac
      121 cgcgccctcg tagttcaacg gatagaacag cacactccta acgtgcggat cgaagttcaa
      181 ttcttcgcga gggcacatgc atatgagacc gatcgagagg cgtcaatgcc tgctttttga
      241 agctgcccgc cgagattgca catcatacat tgactatgtt ccatcgaaga catctgttat
      301 aatgggaaga tgaacgaccg gcgaaaattt gcatggttgc ggatcgcctt cggagctatc
      361 tggggcattg atgcttattt caaatggcag cctgattttt ttgcgaactt cacggattat
      421 ctttcgggaa gcctcgatgg ccagccgcag cttgtaaaag attggattaa tctctggatc
      481 cgtatcgcgg gagtgaaccc gcatattttt gcgctgtttg tcgcgatcgc ggaaacggcg
      541 atcgcgctcg ccctcatttt cggagtagct tcgcgcgccg cgacatgggg cggtatcgca
      601 atgaacctgg tcgtctggtc caccgccgag ggattcggcg gtccctacgc tgcgggatcg
      661 accgatatcg gcacggcgat catttatgct cttgttcttc tcgccttgtg gttcggccat
      721 gcatgggaag atttatccct ggaaaaatgg cttaaaaaac gaagctttgc aatgaaataa
      781 taaaataccc ttgtgatgag ggtattttat tattttcggg aataatggaa aaagggtaag
      841 gttatttcga taaaaatccg gcgattgatt tgcgttttca atgagatggg tcaatcgcat
      901 ggcttatgat tgcgaggtta cagctcttat gttctttgcg ccacgcttcg cacttgtccg
      961 cccattcttt ctctttatag tgcatcccgc attcggggca ttgatgcggc ttttcatcct
     1021 cttttttttc tctacaataa tgatgattca taaaattaaa aatttggttt tagatcccgt
     1081 ctcaaaagca tgccggcggg aattgcggga aatttccgaa cgagcgccgc gatctcctga
     1141 caaggaatat ccgaggtatt tcgaggaagg aatcgcggtt cgccgcagga aatttccgca
     1201 agacccgcgg actttgcttg aaaaactgct tttgagacgg gttctcatct cattctcatc
     1261 tcatcggata cgcagataaa ataatcagcc gcgcggcgtt acttcattac tccccatgtc
     1321 gcgggatcaa actgaccgtc ggccttgaag aaaaacgcgc cgcgcaagcc gtattttttc
     1381 gcgagcgcga tctcggcctt tgccgactgc gcgtcgggaa atgaagtaaa aagcgtgttc
     1441 atgccggcgg ccgaggaaga cgccactatc gcgggttcgg tggacgaaac attgaacgtc
     1501 aggctgtccg gaatgccgtc aacgacggtc gtcgtcgcat acgcaaagcc gagttcgccg
     1561 gcgctgttcc tggttggcgt aatacctacc ccgtccgcgc gatccatcgc tccgaaaaaa
     1621 gtaaaactcc gcaggcgccg gtacgtcgta acgccgttct tccatgatac ctcatattca
     1681 taaccgtacg tcgggatacc gagcattatc ttttttcggt ttatcgacgc aagcgccatt
     1741 ttcacgacct tcgccaccca gacgggatcg gcaacgggcg cgtaaagatt cccgtttcct
     1801 ttcgacgcat caagcttgac atcaatcgtc ccctggtcgt acgccatcac gcggacctcg
     1861 tcgcaatatt tattaagcgc cgcgtaatct tccgcatact gcggcgttgt cgttgacgga
     1921 ttcaaagaaa gcgacgaaag cggcgtgcgc ggaacaatcg tacacgcgag tgttttcccg
     1981 agcggatgca ggcgcatcgc aagcccttgt aaaaacagcg taaaatacgg ctttgtcgaa
     2041 gaagtcatgc tttcgtaatc gatatcgatg ccgttgaatt tttccgacac aacgagattt
     2101 tttatcatat cttcctgcgc ctgtctcgtc ttcgctttgg aaagaacagc gtaaatacta
     2161 tcgccgttga accacgcgat ggtgggggtt atttttatcc cgagttcgcg caaggcggaa
     2221 aaccatccgc tccacgagcc gttgccgatc ctgaggtcgt ccttaagatt cccgccggaa
     2281 acgatctcat aggaaaacgg gcttatctcg ttcaaagaat taagatgcag agaaatatcc
     2341 gccgcgcccg attttgcttc ccagaacggc agccacacgc cgtagaggaa cggcgttttc
     2401 gcggaaaccg ctgatgcgaa aaacaacgcg acgccggcgg cacagaaaaa aatcgcgata
     2461 taaattgttt ttgttttcat tgcttttcat tatcgcatcg gcggacaata atgcaaagac
     2521 tccgcattcc tgttcgaaag gaacgggctc tcctgaatct ctttcaaaca ggaatgccat
     2581 gtcggcgaca ggatcgaaga agtaaaaaag gccgccttcg cgaataaaaa atgaattccc
     2641 ttacattccg aatgtctggc cggtcgcaag cgtgaaaaga aaattaatga ccggaatgat
     2701 aagctggaat ccgaaaaagg cgaagaaaag gacaatgatc aacccgtacc gctcaaggaa
     2761 cgcggcagcg cggaacgtcg cttcggtctg cggcaacgcg gcgaataata cccgcgaacc
     2821 gtcgagcggc ggaatgggaa taaggttaaa aacgccgagc atgatgttca tatacgcgat
     2881 aaggccaagt aattcaataa aaattgccga aacgttaaaa taaaaaaata ttcttgtcgc
     2941 gataccgaac acggccgcga gcgcgaaatt cgtgaacggt cccgccagcg cgattttcac
     3001 ggaaccgccc cgcggatctt tcagattccg cggatcgtac ggcaccggct tcgccgcggc
     3061 gaggaaaaaa ctgccgcccg atgcgatgaa aagcgtaagc ggcatgataa acgaaccgaa
     3121 aagatccata tgtttcaaag gattcagggt cagccgcccc gcgcgccgcg ccgtatcgtc
     3181 ccccagccgg tctgcttcaa gcccgtgcgc gacttcatgg agcaccgcgg aaaaaataag
     3241 cacgatgaat tcgaaaatgg tgagtacgac cgtggttccg ttcatggatt tcattatagc
     3301 aatattgccg tcgttttgcc attttcaccg gaatcaatat caaaaacaca cgttcggcat
     3361 aaaattgccg tcaaaaaagt ttgacaaaaa ataataggaa tgctatattt agaacagttc
     3421 ttgaatggat attccatgca tgggcacgaa aatccattcc tgtccgataa aaaaagacag
     3481 aacaattgaa tatttaaagg gaggatgttt ttatgaaaaa atcggcgata agcgatacgg
     3541 attttgtttc gacagtagcg atagcgacgt ccatgttcgc tgcattggca ataataatta
     3601 caaccgataa ctcttttgct ataaaggtcg tactatccgc cgccgcatgc tcatgtataa
     3661 gcattgctgg ggttatgtat atcagaaaaa tcagaatcag gcatcaattt aaaaaaaaat
     3721 tcgggtttga tcctggctca gttccgaaaa gttgcctcgc ggaatcaaaa gtggaaaaca
     3781 agcttttgat tttcataatg gagattaaaa aggcgcgcga taaacagagg agcgcttacg
     3841 atatcgatgg ccggtttcct tttttggtaa aaaagaggag cgataaggag ttatgcgaaa
     3901 gagaaaaaaa attccgcaag gcgtatcgcc tcgccatcct tttcgatttt cccgttggca
     3961 gggtcatgga ttacgccaaa ctcaacggta ttgaaatata aagatattcc cgatctttat
     4021 attcacgccg agggcgggtt tgcttttgca gccctccctc ggtatttttt ttatctgaga
     4081 aaacacatac atcaacggtg tacgcgcgga agaacacgca acgacacccg gacgattata
     4141 atattttcca tcaatttacc cgtctaatta agtttgctac tttagataaa ttcttatccc
     4201 cattatttga aggcaaaagc catcaatata atagtcaacg cattaagaat cactaatgtt
     4261 atagctgttc ctaagcgagt tggaagtggt tttataaaaa aatcgtgtaa attatggcca
     4321 caatcactgc gccaataaag tttcctctta ccggcctttc atgattacgt ggatattata
     4381 catccgaaag ccagtgttta cgaaattcaa tccatcccgc gaaaatttta gggaaaatta
     4441 ccttagctgc aactgcgtgc tccccagtac agaatcttcg aacttttttc cctagacaag
     4501 ttacaggagg aagtatcgtc cgggatcgtt atggattaat tttaggaaaa agattcgcac
     4561 aaaataaggt gggagaaaat gacggaaggg gaaataaaaa tggccacatc tttaatatct
     4621 ctttacttat tctttatcag taaataccac aagtctattg gagtaactct tggaaatttt
     4681 attccaagcc ccttcacagg taatgagatt aaggtgcgct cctccatcat tcgagttaaa
     4741 cacgtttggg acgttttcat tccagttata agtcagaacc ttgcgcacga taaaggcagt
     4801 agttatccct ccttcgctct caatgtatag ctgatcacct gggcgtaatt tgtataagtc
     4861 atcaaatacc gccggtatgt catctttcca gccgtaatgt ccgacaataa tagagctccc
     4921 agtttccccc gggcgcgggc tgaggtcgaa ccacgctgcg tcagtgggac cttgcggaac
     4981 tcccactgct ccctgaggag taagccctgt atattctatg gcagcatcaa cgccgatttt
     5041 tggaatcttg agacgaaccg gaagccctat ggtgggttcg gtcgatatgt cgacgactaa
     5101 attttgagcg gtctgatttt tgataaatga aatcggttta gcggcgaatc cataagcggg
     5161 tacgtcgggg acgagaaaaa aaataatcat tgaaaaagac gcgatgacca caatggcaag
     5221 gacgatcaat agtaatttga atgagatttt cgattgcatg gatttttaag gactttacgc
     5281 atgaaggatt ccgcaaggcg gaatctttcc ctaagagaaa tgtcccttat caccgggcag
     5341 tcaaaaaaca ccattgcggt acaagtgcat cggggactcg aaaaacttaa taaacttatg
     5401 caaccggact tcgaggaaca tccggcaagt ttccggaaag aagtcgcttt ccccccatcc
     5461 agaacaaaat cgggcagttc ctcatgagta cgcctttgcg gaatatcgga gggagaccct
     5521 actgtttttg tctcgtatcc caatttcgca aaaatatcag cgatatattg ctcaaactcc
     5581 catggctcca tgcctcgtaa ttgccagatc aaaccgcttt tattacggcg ccaagagatc
     5641 agcttatggc tcgattttcc ttttgaagtc atcaagaaaa acagaaaagc gattaagcgc
     5701 gaaatcgtta aggttcgaac ctcaaaaaac gtaataaaac gtaataacgg caatgcgtat
     5761 atccgatttt atccaaaaat ttcctctgtt gtgcgcggga gaggactcga acctccaagc
     5821 ccttacgggc gctaccacct caaggtagtg cgtctgccag tttcgccacc cgcgcaagat
     5881 accacgaaga ttttaaacca tccgaaaaaa ttcgtccaat gctatttaaa tagacataat
     5941 tcttattgtg ctgtccccgc ccgtatcgtg gtacggggaa ctatgggcgg acgatgacag
     6001 gtgaaggata tgatgacggc aaatttactt tatgactcgc ttataaaatc aaaatcttct
     6061 cctttcaaca cgagtttcac tttgccgccg cggacgaggc gcttcgtaag aatggcttcg
     6121 gaaagcggcg cgcgcagctt ttcgtcaatc acgcggcgca atggccgcgc gccgaacgcc
     6181 ggatcatagc cgagctggac gagttttttc accgcgctgt cgtcgaattc caataccatt
     6241 ccctgatgct tcaccgtttc cgaaagttcg ccaaggttca gccgcacaat cttatcaagc
     6301 tccgccggtt cgagattttt gaaaataatg atcttcgaaa aacggttgag aagctccgcc
     6361 ttgaaaacgt cggtaagccg gtgtttgagg tattcagcga tgtccgtcat cgactcccct
     6421 tttgaaagcg actcgttgat gatgtccgaa tgcgcgttcg aggtcgcgat gatgatggta
     6481 ttcgtaaagt ccaccgtgcg gccgagattg tcggtcaacc gcccgtcgtc aaaaacctgc
     6541 aggaataaat ttaaaatatc gggatatgcc ttttcgaact catcgagcag cacaaggcag
     6601 tacggctttt cgagaaccgc atccgtgagc gctcccttga cctggccgtc gggcgacccg
     6661 atgaaacgga aaaaactttg cttgtcctga tattccgtca tatcgaaccg cgccatcatc
     6721 ttctccgaac cgaactggat gcccgcaatg attttcgcaa gttcggtttt tccgacaccg
     6781 gtcggcccta cgaaaaggaa acttgcgata gggccgccct ggcgcgtaag accgctccgg
     6841 tactcgcgca atgcctgcga aaccgccttc actgcttctt cctggtcgac cagccgttca
     6901 tggatgatat cttccatatg cataagcttc tgcacttcgt cgccatcggc ttcatgaagc
     6961 ggaatatttg ttttcgcctc cgcgatcgcc gtcacgcggt cgggacccag cattttttct
     7021 ccgcggcgtt ccgcgtcaat cagcgcgctt ttcaaaagct cttcggcgct cgacggcaaa
     7081 aacttattcc tgaaatatct tttcgcaagc aggaccgcgc gcttgatggc gccgaagctg
     7141 atcgtgatcc gcgtcccttt ttcgaggatc agactttcgt acgaaagaag tttttccgcc
     7201 tcggcgatag tgatttcatt caccggtatt gtttcaaatg cgcccacgaa atcgctgcgc
     7261 ggctcgacga gctgcttaaa ttctttcgga tacgtggaac cgatgatggg aaacgtatta
     7321 ttcataatga cgggcatcag ggcgtcggcc gcggaaagat acgcagtgcc ggacgtcttt
     7381 acgagattat ggatctcggg aatataaagg atgatattgc ccgccatata aacttcctcc
     7441 acgatgcgcc gtatccgtgc gttcaattct tcgggcggcg caccggcaac gagattctgc
     7501 agttccaggc cgacaagccg cctgtcgaaa agcgcgctgg gaactttatc gttcgccaga
     7561 tgttcggcaa gatgccgcat catcgcttct tttccgatgc ccgcttcgcc cacgagaata
     7621 acattcgggt tgatgggacg cgcaagcgtt tccaccatcc tctcatattc cgccgcatgg
     7681 ccgacgagaa atcccgccgc tccttcgcgc gcgagatcgg tataatccgt gctgtacctg
     7741 tcaagctcgg gcgtcggccg cgacgtccat gcgcgattca tgatgcggtg cttgatgtac
     7801 cggtgcgatt ccgtcgtaaa accgccgagc accgccggca gacggttcag tatcccctga
     7861 tgggccatgc ttaagataag cgccgtctcc agatcggcgg catcgatcga aaaagtattg
     7921 aatatgcgcg cgatatactc gccctgcatt ttcccgagtc cggcaaaaag atcggcaagt
     7981 tcgataaaat catgtcctgc gacgagcgct tctttgaacg cggcgacgac gagcgcttcg
     8041 gcatcggcgc gataatactc cgccgtcccg cggtctttat ttttgctttc aagaagcaac
     8101 ccgtccaatt tctgtctgaa ctcttcgggt gaaacatcaa gacggcgcaa cccttcttca
     8161 atctgcggat agccgataag ctggcgcgcg gtctccagaa aaaaatcctc cttcgtgatg
     8221 atgctccggt cgaacgcccg ctccagaacg gaaaaagtcc gcggccgcac gaccttcgcc
     8281 aaattgattg ctccttccgc gggaagttcg atgaacggga tatctgcttc gccgtgatgc
     8341 ctcagccggt cggcgaaaaa aaggcacagc aataacccga tatagcgaaa accgcggagg
     8401 tcggattgga agagaaaaat cgccgtcgcc gcgatgagcg tcagataaaa aacataccgt
     8461 atgatgcgga taagaatcct ccccgcgagc gtcataccga aacgcggatc ggaaaaaccg
     8521 acggtcagtt ttttttcttg tttttgggaa gcaacagaag tcatattggt attgcagcgt
     8581 ttggatattg taatgaaaat aggtatcgag atgctccatt atcagaacgt caggacgggt
     8641 tcatcctcgt accgcaagaa taataagtac ggccggaatg gcgatccaga cgagatataa
     8701 aaaaaggaag acaatcgcga acacaatgta aacggcgccg ccgatcgcaa cccttatcga
     8761 acggaagata atcccgagaa tcctccccac gatgctgtaa tctttataga gcggatcgaa
     8821 aaaatgccga agcgtaattt taaccgcgaa cgatttatcc atttccgtga gcacggaaat
     8881 gaatttgtgc gctataaaac gggttccgtc gacataccag tgatgaaaga aatcgacgaa
     8941 ccgataaaga aaacgctgga tgatataaat aaaagcgaag ttcataaaag tattggcatg
     9001 gtgtagtgtt gttgtgccgc atatccgatt ttccgataga atactataac attacatcat
     9061 gacaactcca tttaaagtat agcagaaatc ggataatcaa gcgcatccgc gcagcacgcc
     9121 gtacggtcat agaacccgtc tcaaaagcag ctttttaagc agtatccgca ggtcttacgg
     9181 aaattttatg aaactctgtt tcatccgccg gttcgctctg agaaaatgaa tgcgcggacg
     9241 catttatttt ctcctcgctg ccgacggccg gcaatccccg ccggcatgct tttaggacgg
     9301 gttctaaaac aacgctctct gcttttcttt tcccggattt ttgttttctt ttcgcgcgga
     9361 gccgatggaa aatacggggg tcgcgattac gggaataaaa tcgcccttaa tgatattcgg
     9421 caatttttta aaatcttcct tcaaaagaag catcatctgc tgcggcgacc ggagcgccgc
     9481 cgcgggatgc aacacgggaa aaatgagata atccccagcc ggtgattctt cgccgggacg
     9541 acgaaaggca ccttcattct ctgcttgctc aaacggctcc cggggaacgg caaacacccg
     9601 gccatggtcg cgcatcatct tcgcatcggg aagaaaataa tgcatcgcga accggccgag
     9661 cgtaacgata accggcggac ggatgatggc gatctgccgc gcgagatacg gcttataagc
     9721 cgcaatttcc tccggcagcg gatcacggtt atccggcgga cggcgcttta cgatattcgt
     9781 gatataaacg tcttcgcgct tccaaccgat gtttcgcatc atcccgtcca acagctgtcc
     9841 gccacggccg acgaacggcc ggcgcaaacg atcttcgttc atgcccggcg cttcgccgac
     9901 gaacatcacg gcgcaatcag gatttccctc gccgaacact aagttcgatt cgcgcaacgg
     9961 caacgacgca tccgccacca tttccgcctc gagtttttta agctcagaaa atttatccgc
    10021 cataaaaaca gtatagaacc cgtctcaaaa aaaatgaagc tccgcgcgct cacgcggcgg
    10081 ggtatagcac tcgtttcaaa ccgccggaac ggtttgcgcc gcaaagccgg cggcgcgccg
    10141 ccatcccgca aacttacgca cgtgatatta gtgagcgaag agaacctacg gctgcctatc
    10201 ggactatctt gatataagtt ataggctttt aaatacagcc aattcgccgc aatccgtttc
    10261 ggcatttaaa tcgctgcgaa tttccacaag cgttccctcg actttttcca gccgtacttc
    10321 gactttccct atccgcactt cgagtttttc gagccgtcct tcaattcttt ccaatcgtcc
    10381 atttattaca ataatctcgc cttttatccc gtttatttct tcaaaaacaa tgccgaattt
    10441 aaaatcaaca atcgcaaacc gttcattcat ctcttcgtga agatcatcga tctttttatc
    10501 cagcgcggca tatccttcca aaaccaagtc gagtttgtcg tcgcgttctt ccaaaaggac
    10561 accaatcttc cttatttcga catcatggct ttccaaaatg acgcctatct tttttatttc
    10621 aatatcatgg ctttccatga ggacaccgat cttccttatt tcgacatcgc gatcgtccat
    10681 gcgagaacct attttcttca cttcgctgaa aagttctttc aacccgatat tattgccgcc
    10741 ggaatttttc tttttcattc ctccattcta tccttccgat acctcccgtc aaaatgtctt
    10801 taatgatttt ttaatcaaga ttcattcttt tatttccgca ccagacggag catttttatt
    10861 cgcgaaggag caaaaagaac ccctcctttg tttccgcatc agacggagca tttttatttt
    10921 tgagggactt aagagaaccc gtccctcgtt tcttatttcc gcatcagacg gagtattttt
    10981 atttttgagg gacttaagag agcccgtccc tcaaaaataa aaatgcgaag tcccttatcc
    11041 gtgccgcacg acgacaatat cgccgtcccg cacttcgtat tcctttccct caagccgcac
    11101 ccatcctttc tgcttcgcct gcgtccagcc gccggccgcg agtaattttt cgcagtcaat
    11161 gacttccagc cggatgaatt ttttctcgaa atcggtatgg atgacgcccg ccgcctgcgg
    11221 cgctttcgtg ccattctcaa tcgtccacgc gcgcgtctcg tcctcgcccg tcgtgaaaaa
    11281 acttgcgaga cccagaactt cgtacgcttt tttgataagc tcaggaatct cccctccgga
    11341 accttctgcc gcggcggaaa gatctgcgac gacaaatccc acgccgcagc ggtctgaatt
    11401 ttgttccgca aactttttgg gggcaagcga tgctatttta tttttcaacg catcaggcgc
    11461 ttctttttct tcgccgttca ataaataaat ctgccgcttc attgtcaaca agttcagatc
    11521 gatcatcctg atatttttgc ggatgctttc catacattcc gcgtccgcaa ccggcagatc
    11581 gcctttttca agacgcgctt tcagattttc caaaagctcc ctgtcctcac gcgcttcctt
    11641 cttgccgctc cgtgcttccg cctcggcttt ggtaagccgc ttttcgaccg tttcaagatc
    11701 tttcaaaata agctcggtat tgataatttc cagatcgcgc aaaggatcga cggaattctc
    11761 gacatggatg atttctgcat tccgaaacat ccgcaatacg attacgatcg cctgcgtctc
    11821 gcggatgtgc gacaggaact ggttgccgag tccttcgcct ttattcgcgc ccctcacgag
    11881 tcccgcgatg tccaagaact ccacgaccgc cggaattttc tttttcgatt tactgagatc
    11941 gttcaatttt tcgagccgct catccggcac cgcaacgacg ccgacattgg gatctattgt
    12001 cgcaaacgga tagttcgcaa tcgtcacatc ctgtttcgta ataagtttaa acagcgtcga
    12061 tttcccgaca ttcggaagcc ccacgatgcc gatggatagt tttgccattg caccactata
    12121 acatacatgc ggataattta tgaagtttcg cgcggccata tggccatatt caacgcgttg
    12181 cggaaatttc tccatccttc gcgttaccgg caagaaaacg gcgttaagct cggagtaaaa
    12241 taacaaaaaa agcgctcctt ttcaggggag cgccttgata ttctttcaaa taaaactcaa
    12301 tcatattttc aaccgaatac tttgtcgaaa atatgattga cgtgctgtaa tttttttgtg
    12361 agattgaaac acgattttaa atctttctcg ctaacgaacc gcatgacgat atcgttggcg
    12421 agcaaagctt ccaaaaaatc aaaatctaca gcaagccctt tcgcctgatt ctcccagcat
    12481 cccaacgcaa tgtcgcgaat caaggtatgc gcctcttccc ggggcaagcc gcttttttcc
    12541 gcaacaagca tcataacatc ctgggaaaag acaagcccct tggtgtgatc aatattaatc
    12601 agcatcctgc cgggaaaaac cctcatcttt tcgatgatgc cggtaagccg gtctatggca
    12661 taatcaagga gaatcgaaga atcgggaata atgactctct ccggaccgga attgtccaaa
    12721 ctgcgctcat cccagcacgt tgcaaggttt tccagggcaa cctggacatt cgcgcgaacg
    12781 acacgcatca ggcccgacaa attttcactc cctatcgggt tcttcttgtg cggcatcgcc
    12841 gatgaacctt tctggttcct gccgaacggc tccataacct cgccaatttc cgtttttgaa
    12901 agcaacctga gattggtggc tatcttgccg attgtggcgg caacgatacc taacacggac
    12961 atatactcgg ccgcgatgtc gcgcgcaatg atttgcgtgg caatgacagg tttaagccat
    13021 aattcttcgc aaaccaattc ctcgattttc ggatcgatcg tatacatgcc gaccgctccg
    13081 cttatcttgc cgaccgcaac aacgctacaa agcctgataa gcctgtcccg atgccggtga
    13141 agctcatcat accaattagc cagcttgacc ccgaacgtaa tcggctcggc atgaacgccg
    13201 tgcgtgcgtc cgatctgcgg cgcgtactta tactcaagag ccttttccct gaccgcatcc
    13261 ataagtttca tgattccctc gatcagcagc ctgacgcttt cgcgcatctg catgctcagc
    13321 gccgtgtccc cgacgtcgta tgatgtcaat cctctatgta accacggacg aagctcttcc
    13381 ggaaactggg gcgataaatg ttcgagaaac gcaatcacat catgtcccgt aatctctttt
    13441 tcaatgcggt tgatttcctc gggatcaatg cgaatttccc ccgcaaaatc ccaagaacca
    13501 tcgtatataa gctcgcctat atgctgcctc gccctgagta cggccagttc caccgacaac
    13561 catcgcaaaa atttgttccg ttccgaccag atgccggtca tttctttcct gctgtacctt
    13621 tccaacattg atttcctccc ttgcgcattc cgcgctttta agatttttat ctttattcaa
    13681 ttttaaatga gctgaaaaat gaccgttgaa cggtcgtttt tgtaatgaaa atattaccac
    13741 gcacgcggtg gatagtcaat gattttttgc tttcagaatc agcgccgccg ttttgtccgc
    13801 gccgattttt tccgtatcaa tgataatgta atctttttta tcaaggtatt tttggcaata
    13861 accataaagt ttctcggtta atttcccgct caaattgcct tgtccgaatt cccctttccg
    13921 ttttttattt cttttcagga ccgatgccag cggcgcatca agaagaaaaa cacgggctcc
    13981 cattttcttc aaagaaagcg ccagccattt atcggttttt cccttatcgt atatcgtggg
    14041 cggcgccact tcgataacca cgttatatcc ccgtcgcacg tattccttcg ccaaaagcac
    14101 ggctgttttt ttccctaatg ttatatgggc atcgctttct ttccaactgc caaaacacag
    14161 gcacgtcatg tcccgcaata tgtccacgga aattctcgcc gttttgccgg gtaatttcct
    14221 gcaaagtttt ccggcaaccg cggtttttcc cgttcccgca ggaccatata acgcgatgat
    14281 tattttattt ttttgcgcca ttttttaata tcgcttttat ttttatgtca gaaaattcca
    14341 gaacggcggg attgtccaat atcagctccc actttttttg cgcgaccgga agatcttttt
    14401 ctttccgata atcgacgcaa aaatcgaaaa gtccttttcg cgcattcctc acgtactcaa
    14461 tatgcctgaa ctggtcggaa agctcccgcc acggatacag caccaatccg ccaagcccga
    14521 agtgcttcat aagagcggta tccgcgtcgc ttatgaccgc cactgcttcg tctttcacgt
    14581 ttattttatt tttcctgaaa tattctttta tggcggcgat tttggtcatg aatttcaccc
    14641 cttcgcaaac cgcgtttctg tggctgatga tgcttttcac ttttatcgcc ttcccttttc
    14701 ctttttcatt gaactctttt tcttctccga cgcacccgtc gaattttccc ttctctccca
    14761 ataattttaa aacattatcg gcgataattt ccccgtagac tctcaaattt gacgtaagaa
    14821 agataacctt aattttattt tttctcttta tattgcccaa agcgcggaca agcggctcat
    14881 tgatgagctt tttttc
//
LOCUS       DAIDDT010000016        14781 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_16_length_14781_cov_6.739799, whole genome shotgun sequence.
ACCESSION   DAIDDT010000016 DAIDDT010000000
VERSION     DAIDDT010000016.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14781)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14781)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14781
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16_length_14781_cov_6.739799"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3614 a         3948 c         3439 g         3780 t
ORIGIN      
        1 caaatagata gggcgttatt aatcggtcgt tgccgcgtcc cctccgtcgt ccttgcgaag
       61 actcgcccta tggagtggcg acttcgccta ctccacaggg cgaggatcca tgcttttaat
      121 cttctttctt ctacttaatt cttaaagcgt ggattctcct tttgaggagg acgacggaaa
      181 agcgtaggct ctcatcttct cgagaaagta agcttatgtt tattttcatt gtttttgcat
      241 cagcgcgctt acggcacttt gcgcaatctg gagcgcctga ccggaactca ccgatccgga
      301 aagcgcggat tgaatggcgg tattgaagat gccgtcgatt gcgccgctgt ccgcctcgta
      361 ccacgatcgc gccgtaagcg cctgaaaagc aaacgtcgca agatcgggat tgctttccgc
      421 agcgcttatc gcgattctct gcgccggcgg aaatccggta tccttcagat aaatactctc
      481 accttcggca tacgccgtca aatagaggac gaaatcccat gccggcagca cctgcccttg
      541 tttcgaaacg gcgagtccct ggtatttcgg atagtttacc gcaatcgtcg catccttcgg
      601 ctggggcatc gcggcgatgc cgatattcaa aaacggcgcc ttcgctttta tcgccgcaag
      661 cgaagattca taatcgaaaa tgatcgccgt tttcccctgg atgaaactct gggtggcgtt
      721 tcccatattg tcgttccacg tgtaatacgg cgacgcggca tccgcgaact ggaggtaaaa
      781 attgaacgcg gcgaggccgg tgcttcccgc gcccatatcg ctcgcgaatt cagccgaaga
      841 aaaatcggca ttcgtcataa tcgtgccgtt ttgcagcatc aaaagagaaa ggatatcggg
      901 cgcgtccgcg atacttgttt cactgcctcc caaagcagcc gcggcgcgga cgatctggcc
      961 ctggctattc aacacgcgta acttcgtcac atcggtttca aaatcatccc acgttacggg
     1021 cggcgtcgcg attcctgcgg aattgaaaaa atcgcggtta tagatcatgg cgagcgtgtc
     1081 gatggaaagc ggaagggcgt agatctgtcc gtcggaaaca aaatcctgcc cgacaacgtc
     1141 cgggaaataa ctttgcagtt tcgcaagatc gaattccgcc gcgaaggtcg acgtcggcat
     1201 gggcgcaagc gccggctccc attgtggcag gtcgcggttc ccgatctcga aaacatcggg
     1261 gccgttcccg tccgcaagcg cggacagcac cttgctttga taatcggcgg gatctatagg
     1321 agtataggta accgtcgtgt tcggacgata ggcgtcataa gacgttataa ggtcctggaa
     1381 aactttcaag ggctcggttc cccacacggt caatgttatc ttcggcgccg cctgttgttt
     1441 tttactgccg ccgaaaaaaa gaaaaagaaa tatgccgatg acaataatgc cgagagcggc
     1501 gaggatcatt atttgttttt gggtgaactt gggcatgaga agaagtaaaa agtgaaagtt
     1561 gatgaattcg caaagcgaat gtccgcattt ttcactttta gtttttcatt tttcactttt
     1621 agtttttcat ttttcacttt tagtttttca tttttcactt cttcaaatca ccacggttgc
     1681 cgcgatgcgg tcgccgctct tcatgtccat aatccgcact ccctgcgccg cgcgtcccgt
     1741 ctcccgcacc gttttcaatt ccgtgcggat catctgtcct ttcgccgaaa gcgcgaatat
     1801 ctcggcctca tccgtgatca cgtgcgcgga aacaagcaat cccgtcttcg gcgtaatttt
     1861 cgcggtcgca atgcccgaac cgccgcggtt ctggacctta taatccttga gcggcgtctg
     1921 tttcgcaaag ccgttttcca tgacaaccag cagtttcgcg tccgctcctt cttttattat
     1981 atcaaatccg gcgacctcgt cattcttttt tttcaatctt atcgcgcgga cgccggccgc
     2041 cgtcctgccc atcggccgcg cctgcttttc gttaaacctg accgactgcc cctgccgcgt
     2101 cgcaagtacc gcctcgctcg atccgtcggt gagtttcgcc cactgcaaat gatcgccctt
     2161 cttcaatgaa atcgcgatga tgcccgtgcg gcggatattc gtgaaatcgt cgagcgtcgt
     2221 ttttttcacg acgccctgcc tggtcaccat cataagatat ttccgctttt cccctttctc
     2281 ggaataattc acgattgcgt tcacattttc gtccgccggc agttcgagaa aattctggat
     2341 cgcttttcct ttcgatgtcc gcgtcgccgg cggaatctcg tacactttcg tctggaaaac
     2401 gcggccgcgg tcggtgaaga aaaggacatt gtcatgcgtt tttgcgctga aaaactgcga
     2461 aagaaaatcg tcatcggcga catccgaccc gataagcccc ttgccgccgc gccgctggac
     2521 tttgaacgtt tcgggcggaa ggcgcttgat atagccgccg ccggaaaccg tgacaatcgc
     2581 atcctcttcg ggaataaggt cttcgtccgc gaccgcttcc ggcgcatggg tgaccacccg
     2641 cgtccgccgt tcgtcgccgt atttttcttt cagctccttc acttcgtccg tgatgatacc
     2701 taaaattttt gcggggcttt tcaggataag catcagcccg gcaatcagcg cgcgcttttc
     2761 tttgagctcc tcgtctattt ttttatgctc cagcgccgca agagtctgga gccgcatctc
     2821 gagaatcgcc gtcgcctgaa gatcggagag tctaaaaagc ttcatcagat tcgcatgcgc
     2881 ctcttccttg tctttcgatt tcttgatggt tgcgatgacg cggtcgatgg catcgagcgc
     2941 gatgacgagg ccttcgagaa tatgcgcgcg ctcctgcgct tttctgaggt caaactgcgt
     3001 ccgtcggcgg acaatctcct tccgatggac gatataggcc tcaagaaaat cacgcagcga
     3061 caaaagctgc ggctggagcc cgccgctctg cggcacgagc gccagcatat tcatattgaa
     3121 attcttctgg aggtcagtgt gtttatagag ccaattcaaa attttctgcg gcgccacgtc
     3181 gttcttgagc tcgatcacga tccgcatccc ttcgcggtcg gactcgtcgc gcagatcgcg
     3241 gataccttct atttttttat cctgcgcaag ctcggcgatg gatttcacga gctccgcctt
     3301 gttcacctgg tacggaattt ccgtaaccac gatatcgaat tgtttgccgc tctttttttc
     3361 aacgatctcc gtcttcgcgc gcgtcaaaac cgcgccacgg ccggtgatat acgcttcttt
     3421 gatcgctttt atatcataaa taatgccgcc ggtcggaaaa tcggggcctt tgacaaactg
     3481 caaaagatct tcggtggtcg catcctggtg ttccgcgaga taaagaatcg cgtcgcatac
     3541 ttcgttcagg ttgtgcggcg gaatgctcgt cgccatgccg accgcaatgc cgaccgtacc
     3601 gctcaaaagc aggttcggca gtttcgccgg aagcaccttc ggttcgtcgc gcgtcgcatc
     3661 gtaattcggc atccaatcaa ccgtttcctt gtcgatatcg acaagcattt ccgcggcgat
     3721 cttcgagagc cgcgcttccg tataccggta cgccgccgga ggatccccgt cgacggatcc
     3781 gaaattcccc tgcccctgca cgagcggata ccggagagaa aaatcctgtg ccatgcggac
     3841 catggcgtca tagaccgagg tgtcgccgtg cggatgataa cgcgcaagca cctgcccgac
     3901 cacattcgcc gatttcctga acttcgcgct gtgcacgaga ccgctgtccc acatatccca
     3961 caaaatcctc cgatgcaccg gcttcatgcc gtcgcgcaca tcgggcaatg cgcgcgaaac
     4021 gatcaccgac atcgcatagt cgagatagga ttcctgcagc tcggtgatta tttccctttt
     4081 ttcggcgtcc atgattttaa ataaatttga tctcttttgt cgtcctcctg aagaggagga
     4141 tctccgcttc caatcttatt tcctgaatct taaatcataa atcgcggatc gtaaaattca
     4201 taaagcgcgg atcctcgccc tatggagcgg cggcttcgcc cactccatag gacgaaaaca
     4261 actgataaaa tcttcatcgc tactgccccg cggaaggata tccggcggcc ggtgttccct
     4321 gcggcgcgct gacggcaacg aacggattga aatacgcgag ccacacgaaa ataataataa
     4381 ccatacaaac gatggtcaca acgatagcga ccttccgttt cgtcggttcg tccgccgcgc
     4441 gaatgttttc caaaaaattt tttatcatgg tgccgtttat gtttttatgt taatagaact
     4501 tacgtacagg ccgctgtagt ttgaaatatt gtcttatgat tttttcaaat gcggaaattc
     4561 catttaactc tcctttatta tcacggggcg gcccttgtct cccgcaatat ctttctgttt
     4621 gaaaacgaat ttttcgacgg aatccgtttt tttgccaagc gccttcaaaa aatcgtcgtg
     4681 attcttcgca aaacacggaa caacgatctt cgcctcaagc tgttttacca tcttcgccgc
     4741 aacctccggc tcaaggtaat gtccgccgcc tacgggcaaa aaaagaacgt cggcctcgaa
     4801 aaattcttcc ataagcgttg cgttgagcgg ccgcgaaaga tgtcccaaaa aaacgaattt
     4861 catatcgtcc catgcaacga cataggccgt cttcagaaat ttttccgtcg attcctcggc
     4921 gaccggaaat cctgaaatca taatgccttt cgtctcgtac tctcccggga aagcgattgc
     4981 gcccgcggat aattcctccg ctatcgtttc ccgcgcggtg gacgtcagcg tcttcagtgt
     5041 tatatccgcc ttgaggcggt tgttctccgg atcgacgaga acgaccgtat ccccgctctg
     5101 caatctgaaa cacccgttcc caaaatatgc aacgatcata atagagccat tataccaaag
     5161 aagcggcgaa aattccagcg gcaattttat ctttcgcgca cattgcctta aatggcggac
     5221 tcaaccaaga acaggcaaga aagacctttt tctgacgagc ttttccgata atcgggacgg
     5281 tccggaatgg cagttcaaaa tttttgtcga cggggcttgc ctttgctctt gctaaaaatc
     5341 aaacttcctt tataatggct ttcatgcaac aagtggaaga aaacaagacg gttttgacgg
     5401 cgctggcgca gatgatgaaa accgaactga cggcgaccga ctggccgcgg gaaggcaccg
     5461 tggttgatgc gacgctcatt aaaaagcttt cccgtaaggc gtatttcgac ctgggccgct
     5521 tcggcacggg catcgtttat ggcgtcgaaa tcggaaatgc aaaagaaacg ctgcgcgcca
     5581 tgaaaaccgg cgatgcggtg cacgcgaaga ttgtcatgct tgacggcgaa gaaggcctta
     5641 tcgaactctc gctttccgaa gccggtaaac aaaaaatctg gcaggaagcg aaagatctta
     5701 tggaatcggg cgaagtggtc aaagcgaaga tcatcgcggc aaacgccggc ggattgatgg
     5761 cggcgcttcc gggcgagatc aaggcgttcc tccccgtttc ccagcttgcg atagaacatt
     5821 atcccaaagt gcaggacggc gaccgcacga agatcacgga agaactcaaa aaattcatcg
     5881 gcgaagaact gagcgtgaaa ataatagacg taaacccgcg aaccaataag atcatcgcct
     5941 ccgaacgcga agtgctttcc acgaacgtca aagaattgct ttccgcctat gccgcggggc
     6001 aaacggttga ttgcatcgtt tccggcgtgg cggacttcgg cgtattcgtc cgtttcgcgg
     6061 acaatcccga cattgaaggc atgatccaca tctcggaact tgactacaag ctcgttgaaa
     6121 atccgaaaaa cctcgtgaag atcaacgacg cgctcaaagc gaagatcatt gacataaaag
     6181 aaggccgtgt tttcctttcg ctcaaagcgc tcaaagaaga tccgtgggag aaagtcggcg
     6241 accagttcag gcagggcgat gtcgtggaag gacgcatcca taagctcaat ccgttcggcg
     6301 ccgtcgtcgc gcttccgaat aatttccagg gcatggtgca cgtttccgag ttcggcgggc
     6361 aggatgaaat gaaaaaagcg atcatttccg gacaatcgta tatgttcgtc attgatgcca
     6421 taaagccgga agagaaaaga attatgctga agatgaagaa ataggctcgc cataagcatc
     6481 gcgaacaaaa actcccgccg gcgggagttt ttaattatga taagatagtc gcctttgctc
     6541 cttgcttcat tcaaaatttc atccgactga aaaaatattg cgacgaaagc gaaaatacga
     6601 taagataaaa accactccgc gctggaagcg gtggataggc cgggctagct catcggtaga
     6661 gcgctgccct gaagaggcag gcgtgcccag ttcgactctg gggtccggca caaaagaaaa
     6721 taaaactccc cgcggcaaag cagacgggga gctttattta gtaaataata ttttttgatc
     6781 ctttcggcat caaaatcatt gcttggtcgg gccgctggga atcgaaccca gtctatctgc
     6841 tcccaaagca gacgtactac cggtatacta cggcccgttt tcgccgtaaa gcacagtgcc
     6901 cggctataac gagttccttc acaatatctt attgttgttt tagaatcaat gcgttgacgg
     6961 cattaatgcc ctcgtagctt aatggataaa gcaggactct tctaaggtct tgatgggggt
     7021 tcgattccct ccgagggcac aagttggaaa atggcaagtc gtttatatct tctcgcggat
     7081 tcggaaattg attgaaattg atggagtcaa aaaagaatat aaagtgctga aattttattg
     7141 cgactgggcc ttgcatgcag aaattgagga catggatgcg gttaaagaca tcattgatag
     7201 aattctcgcc ggagacaaag agaccatcat gcactttgtg cttgccttca atggattcca
     7261 cagagcattc attaagtttt tagcggccta taaatgtacg cactcaatct acgacaagcc
     7321 atttacaacc cacgccttca ataagctact atcacagata tataccgata cgcccctcat
     7381 cataaaaaca actcaaaaaa cgaagataat atggaaggga atcgccgatg aatccacctc
     7441 caactatggg ggcagtatca aagttgaaaa gattttatag ctatcgaacc tacgcgtgag
     7501 tcgaacacag aacccacgtc tttcaaggta aataggaagt cgggcagggg ttttgattca
     7561 gagaacgtca gggcacaaga atattacaaa aaaaggaaac aagaaataaa aaagcccgga
     7621 aaaaatatcc gggcctgaac catatcctct taaaagagga aaaattatat caaatcacgt
     7681 acgtcgtaca cgggcgcaac ctcaagcgga atatcttgca accgctcgac aatgctgtgg
     7741 agctcgggag gaacgactgt ccagcgatca ataaatgttt tcgcgcgatc ataatcgccg
     7801 gccgactgaa ggttcagata ttccgcgctc aaaagtatca tcttttcgag tgagcgattg
     7861 gaatcgatgc gaaacactcc gctcttcgcg tcataatgca tggcgccgta ttccgcgagc
     7921 caattgtact gcatgagatt gcctttggca tgcgcttcga cataaccatg cctaaaactc
     7981 tgcatccatc ccgaaacctc cgaaataata cagccggcaa ccgtcgcgtc gtcaatgatg
     8041 ccgcgttcgc ggaagtaacc gagaagccgc acgccaagca tgtcggcttt tgcttcctca
     8101 atacaggaat gtaaatcgcc aagcttcact tcaaacggca ctgcacggtc gccgtttctg
     8161 accatgctcg gacccaaacc atgcgacaat tcgtgggcaa gaacgaaaag catccttgca
     8221 tggaaattga aatattccat atcttccgga gcaagaatcc gttcggcaat gggacgattc
     8281 agggcttcga acttcgcccg catcatcgtg cttgaaaata ccttctttga acccttgatc
     8341 tcgtgcacgc gccggtcatt cggcaggcta taagcgataa atctgtaacc gaatgccgct
     8401 tcgccgccgc ggtagacgtc ggcgaccacc tcaagcggag tcacggcgtc tttcggtttg
     8461 aagttgaact ctccgctcaa tatgcggtcg aaatcgggga tgacgggtat gaatttcgcg
     8521 agcgccgccg ttgcgtcttt gtcaggcaag ccgataaacg ccgtaaatgc ggctttcaaa
     8581 ccgagaagtt cgtcgaaata tacttcataa ggaccgatgg tcacttcaaa gggatctccg
     8641 gttgtgtcga cccaggaaag gtcggaactg aaatagtcat tcgaattgaa aaacgactcg
     8701 gcgcgcagtt taagaaatga ctggagattc cccttgggca attccttcgc cgccgaccaa
     8761 agctcgttgg ccgcttttcg gagagaaacc ttgtaaattt tcgcatacgg aaacgcaata
     8821 agcgcattcc cgcgccggtc gatcgccgta tagctacttt caaacctttc tctgtcttcc
     8881 ggatgcgccg cgagccatgc attccactcc tctttcgtga aatcccgtgg atagaacgat
     8941 ccgaatttcg gcctgggacc gacgccggga acaaacggtt cgttgcggac atactgatcc
     9001 catggagatc cctgcacccc gaaatagcgg gcaaggtctt ttccttcggc atcgctccgt
     9061 aaggaaatgg cacgatacat ttcaaaatta ccgtgggcaa cctggcgaag atagatgtcg
     9121 gtcataatct ttgccgcacg gacacaatgc cgtaatgcac ggcgttcttt gtcgctgaga
     9181 gcggaaaggt cgggcttcac cggaaaaaaa cggaccttcc cgagacgttc cttaatatcc
     9241 ataaatcctc cttaatttaa gttttttaaa tttttaaaat actgccaata aagtataata
     9301 aaacccgata aagcgcaagg ggatgcgctg ggcgatattt attttaaatt ttaagacgaa
     9361 tccccacctt ctggattcga gaaatccgct ttaagataaa ataaaatatc agccgggcaa
     9421 tacccggctg atattaaatg gatgcggaaa gccctctttg attaacggga agaccagatc
     9481 tgggacagtt tttccgcatt acacgccttc accgtaacaa tactggtact tgcctgtacg
     9541 gtaatggcgt aaccggtatc cgatgccgtg ccgcccgacg gatctatcgg cgcacccttt
     9601 aaataagaag catacgttgt cgatgcatca aaacaattcg tgccagatgc aacatcacag
     9661 ccgccaccgg tattggccaa agcggtaccc gctgcgcatg ttcctatcat ggtgtcggtc
     9721 gaagtggaaa tgccagtcgg aagcgttcca ctattatcca gaatatattc atgcgtcgcg
     9781 gtcaaaagag catccacgtc cgccgtcctt ctcgcatcgc gggcctgcgc aagacgcaaa
     9841 gcgggattca aagcgacgaa gacggttacc gccaacgcca cgatcaccgc gataacaagc
     9901 aagagctcga taagggtgaa tccctttaaa tcatcaactg tttttttcat tttaagattg
     9961 ctgttttatt cccacgcatt cccggaatat ctcttggtct tgccgcagag accgatacct
    10021 cgcaagaata cgtggagcct atttttttcc aataattatc ttcacgtcga ccctttcgat
    10081 ctcgcttaaa ggagatcctt cgcttatagt atatcccatt gataacgcct gctcaagcgc
    10141 cgcgcggtaa gcgccgggga tatctttttt ataactcact accgtatccg catagttgaa
    10201 attattagca ttccccgtgg ccgccaccgt aaatccattt ttctcaagaa tgccctgcat
    10261 tatttgcgcc gcaccggaaa cgccgctgcc gtttaaaatc tcaatgctgt aagccgcgag
    10321 atccggcgcg ggcgaagaag aagacgcggt agttgtagta gtggcggcgg atatttcggc
    10381 ggcggaagaa acgtccgcgg tagtggttga aaccatgggg ataccttgag tctgaatgga
    10441 aacatttgcg gaggatgcca taatcgcagc gccgttcatt attttttcaa cctggtttct
    10501 ttgatacaat atccatccgt ttcccgcaag gacaagcaaa gaaagcccga gcatgacgga
    10561 ggtgacaagt tttccccgca gatgttcgct ttcgccgttc gttccgatag tttcgtactg
    10621 ccccgcccac ggttctttga cgatctccga aatgccgaca attttattgc caagccgatt
    10681 ttcctccggt agcggcacgg cgtttaatat gatattcaag ctggtttcgt cctttttgtc
    10741 gatatcggtc ttcaccgcca tcccggaaaa aaatcaagct cccctttttc gacatcccat
    10801 cccgcaaacg cggaagtttt ttcgcttaag atcttgaatt tccccgaaag aaaaacttcc
    10861 ttcgggaaaa aaccgaattt ttcttttaca aactcggaaa atttcacgat ctcttcctgt
    10921 gtcggttcga tgtcgaagct ctccacgaaa aacacaagcc cgcgatacga aaggaacgtc
    10981 aaaaacttta tcgctttatg gatgaccagc accggctccg ggcgtttctc gagcaggcgc
    11041 gccaaggaac aggcgatcgg ctccaccgcc tctattttta taccggaatc tttgacggcc
    11101 gccgaaagat ctttataaaa ttgattatca agcgaggcga attggacgat tttatttttt
    11161 ttatccgcgc tctcgagcac ttctttgaaa tcccagccga agtcgccgat ttcttccgga
    11221 acgtcttccg cggctttgat gcgcacgaaa ttcctttctt ctttggtatc ggcgggaatc
    11281 gtgatgccga acgtgaacgt cagatcatcg tcgacaatga cagaaaaaac gttgccgaag
    11341 tttttttaaa accaatgagt atcgtggcaa gcgtttcctt tatccacggc agttcctttt
    11401 tcgcgttcta ttttttgcga ctggaaagaa ccgctcgcca tggcaagctg aactgtttcc
    11461 ctggaaaaat aaagaatgcg ctttttaggg ttaaacataa tcaaagaaat tccgccttga
    11521 aaatttcggg tttcccttgt ctccctcgga gacgcagtcc acacgccgcc cgcgggacac
    11581 gtgacaccgt tcaaaattcc cctgaataat tatactcttt gaaacgtgcg cggtaaaacc
    11641 aactaaaaaa ataaggcccc ctttgagaga agggggcctt tataaggagg tgatatatca
    11701 gattttccga gtctccaacc ggaaaatccg ttctctgtat ttaaactatc agatccttaa
    11761 tattccgtca accgaatgat cggcggttga tacccctttt ttaaaaacca gcttcaaaga
    11821 tctatcgccg ctatatcctg atcctaaaaa atcgcttctt cccgattttt accgcctcgc
    11881 cgccacgcac gcgcaccatt tccgcggcat ttttaacaac cttttccgca atctcgaacc
    11941 ctccctgctc aataagccgc cgcgcttcgc tcctgctttt cataatgccc gccgcgagca
    12001 caatatcaag cgccgaaaaa ccgcctgttt tcaatatgcc gtctgttttc aatttcaatt
    12061 ccggaagatt gtccggcatt tctttccgcg aaaataattt ctcgaaattt tcccgcgccg
    12121 cctgcgccgc gccggcgccg tgataacgct tcacgatcgc gaacgcgaga agctctttga
    12181 gctctttcgg atttcccgag ctctttaatt ttttctcgaa tttccggatg tcctccatgg
    12241 atgcatcggt cgttttttca aaatattccg gaatcagttc gtcgcgcaat gtcattgcct
    12301 tcccgaactg attatcgggt tcatcctcga tattaatgac attcccccag ctcgtcgaca
    12361 tcttgcggcc atccaaaccg accaccattg cggttgtcat aatatcctgc ggcttttgac
    12421 cataccgctc ctgaaccttc cgtcccgcca aaagattgaa gagctggtcg gcgcctccta
    12481 tctccacgtc cgctttgacc gccaccgaat catagccctg cattaaagga tacatccctt
    12541 cgcggtaact gatttcttcg tgtttttccc accgctcgct gaaattgcgg cgttctaaaa
    12601 tctgctgaaa actgaaaatg tccgcgagct ctgaaatttc gcggaggtcg agcttggcaa
    12661 gccattcgct gttgtaacgc acttcggctt ttttgatatc aagaattttt ccgatttgcg
    12721 ggagataatt tttaagattt tccttgacgt cggcgcggga taaaaagggg cgttttttca
    12781 gtttgtcgga agggtctccg atctgcgccg taaaatcccc gattatcaaa acgattttgt
    12841 ggcccagctc ctgaaactcg cgaagtttcc ataatgcgac ggcccggccg atatggatat
    12901 ttgctcccgt cggatcaata ccgaatttca cgcgcaattt ttcgccgccg atgagccgtt
    12961 tttgaagatg ggcgcggtca atgacatcct cgatattccg cgagagcaat gtttctatct
    13021 gtccgttctt gttttccatc atgcctattt tagcacgaac gcacgcttgg caccgctctc
    13081 atgcgaatgt cctacctgcc ccgtatgatc gatttcactt tcagtttttt cgtcccgaga
    13141 tattccttga gataccgcac cgcttctttg gcatcaaaat cgcggcagga ataaacgtcg
    13201 attgccacat accgctcttc cggccacgta tgaaatgaaa tatggctttc ataaaggatc
    13261 acgaaacccg acagaccggt cgtgggatac tgcggggaac ttatctcgcc gatgaccggc
    13321 ttgcggaata tacgcatccc taattgtccg ggcatctcat cgagaagccg tttcatcttt
    13381 tttttgtttt caagcagtcc gctgtccacc tcgtacgcat caataataag atgcctgcct
    13441 tgaaatgttg tcatcgctca tctttatgat ttttaataat ctcgtcgatc acgccatacg
    13501 cttttgcttc ctccgcggaa agccagaagt cacggtcggt atcccgctcg attttcgcaa
    13561 gcgactgctt ggtgtgtttc gccaaaagct ggttgaggcg ctgcttggtc ttgataatct
    13621 gtttcgccgc aatctcgatc tctatcgcct gtccctcggc gccgcccatc acctggtgga
    13681 gaaggatctc cgagttgggc aaagcaaacc gctttccgtt ctgtcccgcc gcgagcagga
    13741 acgcgcccat ggatgcggca ataccgacgc acatcgtcgc aatatccgga ttcacgtact
    13801 gcattgtgtc gtaaatcgcc attcccgccg tcaccgatcc gcccggactg ttgatataaa
    13861 gtttgatctc tttttttgga tcctcgtgtt cgagaaacag cagctgggca atcaccgtgt
    13921 tcgccacggc atcgttgatg gtcccgccca aaaatatgat gcgctcttta agaaggcgcg
    13981 aatagatatc gtacgcccgc tcgccgtatt gcgatttttc gatgacggtc ggaataagat
    14041 attggttttc ggtatccatg ttgttgttat agtgttctca tgttgtaaga ttgcagtgca
    14101 atgcccgaat gcgatgcgct ttatttttta cgtcgcatca gccattcgca tcagtagaat
    14161 attacaacat taaaactttt tgataaaaac acaagaccat aaaagcgcac cggacgcagg
    14221 ggtgtcgagg atttatttat gaagaggatc ctctttatac accgttgccg ctttcgcaat
    14281 ctccgctttc gccgccacgg cattatccca ccccaaaatc tccacttttt ttccctgaag
    14341 ctctttgtag gtttcaaaaa aatgcgctat ctccttcaaa agatgcgggt tccatgcctc
    14401 aacatccgta atgtctttta ttttttcgaa acgcgggttc ttatcctgga cggcaagaac
    14461 tttgaaatcg gggtctccgc cgtcgatcat ccggaaaaga cccacggggc gcgcttccac
    14521 gagacatccc aatacaacgg gttcgcttcc tataataagc gcatcaagat gatcgccatc
    14581 ctcagaccgc gtttgcggaa taaaaccata atcaagcgga tagtaaaacg gcgaatagag
    14641 cacgcgatcc agtttgaaaa tcccgagccg ctcgtcgaat tcatatttat tattgcttcc
    14701 ccgcggaatc tcaataataa cgttgatggt ctccggcgct ttatcgccga gcggaacgtt
    14761 ttcccaaagg ttatagatat t
//
LOCUS       DAIDDT010000017        14698 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_17_length_14698_cov_7.042884, whole genome shotgun sequence.
ACCESSION   DAIDDT010000017 DAIDDT010000000
VERSION     DAIDDT010000017.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14698)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14698)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14698
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17_length_14698_cov_7.042884"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3743 a         3954 c         3391 g         3610 t
ORIGIN      
        1 gcgtaaccgc cacgccacag ggcagggacg acagaaaaaa aatcgcttat ttgtatttct
       61 tcagcgcatt ccgcgccgct tcaagctcgg catcctgctt cgcggctccc gcgccttcgg
      121 cgataagctt ttcgccaaaa tagacgccca tcctgaaaac gcgctggtgc gccggacccg
      181 attcgccaag catgcggtac gtcggcgtca ctttgagctt ttcctgtatt atttcctgaa
      241 gctcgctctt ggcatcctta taacttcccg tcttcaaaac ctcgtcaaga tgcttcatca
      301 cgaacttttc aacgaacggt tttgttttct tcataccctg atcgaggtaa atcgcgccga
      361 tcagcgcctc catggcattc gcaagaatga cctcgcgcgc gcgtcccgtg tctttctttt
      421 cgccgcgcga catcagaatg aaatcatcca gcgccatcgc ttccgcgacc ttcgcgagaa
      481 tctgataatt gacgagcgcc gcgcgaagca ccgtcagccg cccctcaggc tgtttgggaa
      541 atttaccgta aagcgcttcg cttacgatga gctcaagcac cgcatcgccc agatattcca
      601 gccgctcatt atgcggcagg cgccatttgg gacactcgtt caggtacgag cggtgcgtca
      661 atgcttcagt caaaagatcg ccgttcttga acgtcacgcc tattcttttt tccaggtcct
      721 ttgggttcat agctaaagat aatgcaagaa aaatttttga actgttatcg gcatcgtcaa
      781 aaattctctc tttgtcgtta tccgcgaaga cggataatct gtgcttccaa tcttttcttt
      841 caaaacgtga atccccgcct ccgcaagaac gacggcatta atactcaagg atttcaaacg
      901 gttttgccaa cgttcgccgc cggctcccat tctgcttttt tgctttcata gatcgacccc
      961 aaaacgccgt tcacgaaacg cgatgcgttc gccccgccgt aatttttcgc aagctcgacc
     1021 gcttcattga tggcgacctt cggcggcact tcgccttttt cggcgaacag aagttcgtaa
     1081 acgccgatac gcaggatatt ccggtcgatg atcgcaatct tgtccatagg ccagttcgtc
     1141 gcgttctttt taatcacatc atccacctcg ccaagatgtt cggcgatccc ctgcagggtc
     1201 ttccatgcga actccggttc atcgatgccg ggcgcgaatt cattgaggtt cctttcaaga
     1261 atttccgtaa tatccttttt ttggttgtaa aaatcccact cgtataacga ttggagcaca
     1321 accgccctga taagatgcct cgtagccata tgatttaaaa agaacggcgg gaaaactgcc
     1381 ggcggaacgg tccgaaaaac ttttatttcg ctccgcattg ggcgcaaatc cgatgcgccg
     1441 tcttcggcgc actgcaattt ttgcacacaa aaagccgctc ctttttcatg gccaaatggg
     1501 atcgccgccg gcccactttc ccttttgaat gatgttttac cggtacgcct gccataatga
     1561 ataatgcaga aagtataatg catgcgtcac cgaaaagtca aacttaaaaa tccgcgcata
     1621 atgatcattg tgtcgtcccc acggaggcga gtgcctacgc tttaggaatt tcgaattgaa
     1681 agcatagacc atccccttca gaaggacgac gaatacaaca cagaacagca accggaagat
     1741 aaaaacaata atctctaaca cttcggttgc ttgagatttc ttaaattaca atcaagatga
     1801 cgggcactca ccgtcgtttc caaattcaaa acatcttctt tgagcgatgt ttccttcggc
     1861 tgtatgccga gcgtaatggt aacacgcgga ttccattgat ccccggtatc atttccaaaa
     1921 agaacgacat taaaatactg gacattgaca ttgccgccag tgatatccaa cggttgccca
     1981 tcgttgatat tactgtctct tttttccaga gtagctccct gaaggtaata ttttattctg
     2041 ttcccttggg cgccggtaaa tgcaagaaat tgatacgtcc catagttact gctgttgttg
     2101 ggattgatat tgaaagaact cccggtccgg atctcgcgcg ccatttgttc cagtacggta
     2161 tcaagattgt tctcggcgcc ggcaagcgcc gttatctgcc gctgggtttt aagcgcgttc
     2221 acaaaactgc cgaccgcgat aagaatgatg accgaaaaaa gcgtgagagc gaccaaaagc
     2281 tcgatcagga gatacccctc ctgcgacgca aaatgcggcg cgccggagtg ccgcatcccc
     2341 ttcttcccta ttatattttt ttgcaaaaaa tttttcatac catagttcgc gccgtgttac
     2401 gcatattttt ataaatgcgg ttgccagtta taaaagtggt cctccaaatg gacattttga
     2461 tttgtgatcg taccggtact ccacgtcacg atggaatcaa cggtaatttc atcattcccg
     2521 ttgggggtga tttttatttc tctctgaaaa ttagtcggcg tgccgtttgg catactataa
     2581 ccataagccg gcaggttata accataaaga tggctatgag aatcgaaatc aagcggcaag
     2641 ttagaattag agatataagg aatgagctgt gtacaattgg tgatagtgga gtccaattca
     2701 aaatccttta tatttgcgaa acatgtaccc catcctccgg cgccgccgcc cgcggactca
     2761 tcaaagaccg tcatatcgtg atcgatcaga tttttcgtca gttcgatccc ttccgcggca
     2821 agataggtcg ctttcatgct gtcagaaata tccctttcgt agaaaaacga tctcgaaagg
     2881 agggacataa tacccacgaa accgaccata aggacgctga gcgccaccat tgcctcgata
     2941 agggactgcc ctttcctcat ttttgaccgc atcttggatt tcataaatgg aatcatgtcc
     3001 tatagcgtca atctgttgtg atttcaccgg ccgcattcac ggtaatggtt gacgatgcgg
     3061 tgccatcagc ggtaacaagt tcgatgttta acggattatt atctgaccag gaactgccgt
     3121 cggcgcttat gcgcgtcgta ggatcgggag gataaaaaag aacgagcgcc gaaccatttc
     3181 cattaaactt aacgcctgtc ttgatcggca cattccacgt cgcatcgcta tactgcgcca
     3241 tctgattatt gggaaaggaa ggtaccgaat ttaacgctaa cgcgctatcg ggacacggag
     3301 gagatccctg cggttcatag gtaaaaatgg aatatttatt acgggcatag tcgaaataaa
     3361 cgccgtaccc gcatgactgc gacgttacat acgttgaagc cgcgagttct ttcgcttgat
     3421 agataaattc cgatattttt gccgtctctg aagaaagggc gattgcgttc ctgccgacac
     3481 tgctgtaaac aacagccatt gcggcaagca tcgcggaaat ggcgataacg atcagaacct
     3541 cgataagggt gaaagctccg cgatcggaaa ataaagaatt attgtgcttc atttttcttt
     3601 attttaacat atttcttctc tgtcatcctc gcgcaggcga gggcccgcgt ttttgcttta
     3661 agaattaatt ggaagcatgg gtcatccgcc ttcgcggacg atgacagaaa aagaaatgaa
     3721 tttgatgacg gcaatgcatc acagccccat catatggata taccatccca tcagaccgga
     3781 tccaaaaaag aaaacaaaaa ccgcgccgag cgcaagaaac ggtccgaaag gaagggtgct
     3841 ttttatcgtc ttttttttca gcgcgataaa aagaagcccg atcgcggcgc ccgacacgaa
     3901 agccgcgccc gaaagaaaaa taatatcggg ccagccgaaa agaaatccga gtggaaacgc
     3961 aagtttgacg tcgcctattc ccattccttt cccgcgcgtg aaagcgacga gccccgcaaa
     4021 aaacaaaaat ccggcgacgg ctcccatgat gtgactcgac cagatgtttt cctgaatccc
     4081 gaaaacggaa gcgtacggcc cgaaaaacga aacattcgcg gaaccgaagt acgaggccga
     4141 aaaaccgaca agaaggatgg cgacggccgt taagaatatc gaaatctcgt tagggatgat
     4201 gccgaggcgg atatcgatga ccgaaagcag ggcgagcgcg aaaaaaaacg ctatccaaag
     4261 gacggtgagt gtccagaaac cgccgccggc aatgccgtaa aatgcctgta cgcgcgcgcc
     4321 aatcgcgaca aaaagaaatc cggaaaataa ttctataagc ggatacgcga agctgatgcg
     4381 cgctttgcag ttccggcatt gcccgccctg gatgatgaaa cttacaacgg gaatcaatgc
     4441 gtaccaccgg agcgttgcca tgcaatgcgg acaatacgac cggccgccga caaccttcgg
     4501 attgagcagg aaatgatcgc cgtcgtaccg caacgcaaga acataaccga aacttccgaa
     4561 aacgaggccg aaaagaaaaa gaagtataga tgacatcggt ttattataat acaaaattac
     4621 gtcgatcgat atcaacgcga tcggttaaat aaaaggtccg tcatcttgga cgaggacgtt
     4681 ttcgtccgcg atccaggatc catgtttatt tataatagag aattaattct ggatcctgga
     4741 tattcctcgc aaagcctcgg aattccagga tgacagaggg gaagttaaag cgtaaggcaa
     4801 taatatggtg cagcacagct accgcctgtc ggcgtattgc tataggtact tccatcaggc
     4861 gcagttgtcg aaggaggagt atatccttga aacgaagaac cacttttatt ttcaagtttt
     4921 gcccacagaa gataggattg ttggtcagtc gatacgccat acccatatac ataaccggca
     4981 gcagcgttgt ttgtcgggtc ttgcggcacg gcattgccgc cgataagagc aggcgtcaaa
     5041 tcagcagaag tgatagtttt cggatatctt ccgttttggg cataatataa ttcaagcgca
     5101 gtctggatcg agtgaagatc cgaaacacgg cgggcatcgc ggccgagctg ttcggtgggg
     5161 ccgataccga ccaatacgat agacgccaaa atagcgataa tggcgacgac aatgagaatt
     5221 tcaataagcg tgaaccccgt gcgcgatatt ttttttgcgc aaaatgtttt tcgcatgatt
     5281 tcgttttgaa agttttaaaa ttttcgactt tttatcgatg cttttattat atcatactct
     5341 gctcatccgc aaatccggtt tatccacagt aagtccgtca ttccgaaaaa taagacattg
     5401 aaacggactc ttcttgttta tatcccgttc agcggggccg tttccgcatc aaacggagca
     5461 ttcctgttcg cgaaggaatg ctaagaaaac tcctccttcg ttttctgcac cagacggagc
     5521 atttttattt ttgagggatt taagaaagcc cgtccctcaa aaataaaaat gtgaagtccc
     5581 tcactgaata ctcgccgtca ggcggtaaat cggaaccagg atcgccgcga aaagaagccc
     5641 gaccatgacc cccatcacga tcataagcac cggctggatg atatcgacaa gattattcat
     5701 gatatcgtcc gattcgcgcg tataaatgcc cgaaagccgc gtgaacatct cttcggtctt
     5761 gccggtggtc tcgccgacgg cagccatctg ggaaacgagc gcggggaaaa aattaggata
     5821 tttcgcgatg ctattggaaa gaagttcgcc ctcgcgcaca tcattcgcga tagtgcctat
     5881 cacctcttca taggacgtat tacccaccat gtgttttatg atctcaagcg cctgcgcaat
     5941 gggaatacca ccgtggacca gaagcgccat cgcattcccg aaccgcgcca tgatcagggg
     6001 aaaatagact tttttcgtaa gcgggaacgt tatcttgaac gaatcaaaaa gagcgatacc
     6061 ttcctctgtc tggaaataat cgactatgac gacgcctaaa acgacggccg cgaccgcgat
     6121 tgccggccac cattgcgaaa gaaacgtccc cgaactcaaa agcacctgcg tataccacgg
     6181 caatgcaacg ccattttccg taaagaccgt tcccacctgc ggaaatacaa acgtcaccat
     6241 gataaaagca accgcgacga agagcgcaag aataatggcg ggataggtga gcgcaccggc
     6301 ggccttgctc gccaaaatcc cttctttttc ggtatagtcg gcaagaaaac ccgcaatctc
     6361 atccatattg ccggttactt cggccgcgcg caccatttcc atatagaaac tcgggaaaat
     6421 actcccctgc cgctcaatcg cctgtgaaaa agaaagtccc gaatcgacgt cctgcgacat
     6481 agcggatagc gcgccctgga gcgtcgcatt ggcggtctgg gtataaagaa ttttcaaggc
     6541 attatccaag ggaagacgcg cctgaagcag tgttgacagc tggcgcgtga agacgatgat
     6601 atccttccgc cggggacgct tgagatacgc gacaatacga tcgtaccagt tcgcgcctcc
     6661 caccggagcg acgctcatta cgaaaagacc gtgcgtcgcc agaatatcgg cggcgctttc
     6721 acggctgcca gcttcaacga atccaacctg cagctcccct tcctgcgttt ttccctgata
     6781 tttgaatttc atttcgtatg agttctaata ttgtagatgt tgcagtgtaa atacgcgcat
     6841 gatgacatta ggatataaga acattacagc attataactt ttcaatggca tcttatcccc
     6901 tacgtcctct ccaataaaat tctcaactcg gaagggttca acgaataaag ctcagcattt
     6961 tcaagcgaaa cttctttgtt tttcacgaga tttgcaagcg accggttcaa agtaaccatg
     7021 ccttcctgca aacttgtttc tatgaccagg tcaatctgat agaacttccg ttcccggata
     7081 aggttccgga tcgccggatt cacaatcatg atttccgcgg cgggcaccct gcctccggaa
     7141 atccgcggca taagacgttc cgatacaatg gcgaccagcg tcgccgcaag ctgggaaatc
     7201 acctgttcct gctgttcggg cggaaacgaa tcgatgatgc ggtcaatggt ttgcgaagcg
     7261 gaattcgtgt gcaacgtcga aaaaacaaga tggcccgttt ccgccgcagt catggcggtc
     7321 gcgattgatg tcccgtcgcg catctcgcca atcatgacga catcaggatc ctggcgcaaa
     7381 atggtgcgca gtgccgaatg gaaactcaac gtatcaccac cgacctcgcg ctgagaaacg
     7441 atgcatttat cctgcgtaaa aagatattcg atgggatctt caacggtaat gatatggtcg
     7501 gatctcacgt ggtttacttc atctacaagc gccgctaggg tcgacgattt cccgtgtcct
     7561 gcaggtccca caagaagcac gaacccctgg gaaagccgcg cgaaatcatg aagcaatggg
     7621 ggaaggttta attcttcgat ggtctttatc cttgccggca ccaggcggag cgccgcggcg
     7681 aaatgccctc tctcgaaata aacattcacg cgaaacctcg ccttatcttc caggctataa
     7741 gcaagatcga tctgccgctg ctctatgaac tttatctttt gctccggcgt taaaagttcc
     7801 gaaataatcc cttccgccag ttcgggcgtc aacagcggct ctttttgaag cggcatcaat
     7861 actccgtcaa tgcgtaaggt gggatgcttc cctaccgcaa gatgcaagtc cgaagcgttt
     7921 tctttcgccg tcgtcgcaag aagatcgttt agtttttgct tataatcatc catacgaata
     7981 agtatagcat attggaatag aatatagaaa tcagaattaa gaatataaaa tcgatatccg
     8041 ttttcgttct ttctatattc tatattatcc ccttggtttc atcagtggga aaaacacggt
     8101 ttcgcgcacg ggcttgtcca taagcacggc gaaaagccgt tcggacatcc caaacccggc
     8161 ggttggcggc ataccgtatt caagcgccgt cagaaaatca tcgtccagtt gcattgcctc
     8221 cgcatcaccg ccctcgcgaa gccgcatctg ctcttcaaag cgcgcgcgct gctcgacggg
     8281 atcgttcaat tcggaatatc cgttgccaag ttcggtgccg cacgccacga tctgcacccg
     8341 ttcggcgata cccgggcgcg cagcggacgc tttggaaagc ggcgaaacgg caagcggcgt
     8401 attgaccaga aaacaaggct gtataagatg cggccggacc gtttttttat aaataagatc
     8461 aataagccgc ccgcggccca tatttttttc cggcgcaaga tgaagcgcaa cagccttatc
     8521 gtacagtgca tcgcgcgagg cagcgagagg gtcgatgccg ttcgacttct tgaaaagcgc
     8581 cgcataatcc agccgcggcc attttttccc ccaatctatc tttttgccct gccataccgt
     8641 cgtaagcccg ccggtggttt ttttgatcac ccgcttatac atccgctcga gcatgacgat
     8701 aaggtcgtca taattatgat acgcccaata aaattccata aacgtgaagt cctggaggtg
     8761 ttcgcggtcg ataccttcat tgcggaatac ccggccgatc tcgaatatct tttcgaaccc
     8821 tcccacgacc aattttttca gctgcagctc gagcgaaatt cttaaataaa aattaacgtc
     8881 caaaacatta tgatgcgtga gaaacggctc cgcctccgcg ccgcccgcca tcggttccaa
     8941 aacgcccgtc tccacttcca cgaactcttc gtcaatcaaa aactcgcgga ttgccgccca
     9001 gaacaggctt ttcttgcgga acatttcacg gacatcggca tgaagaacaa gatcgacgta
     9061 acgctcgcgc aggcgggatt ccacatcttc cagcccgtac cattccgtcg gcaacggccg
     9121 caacgatttc gcaagcatca caaccgaacg cgcctcaatg ctccgctcgc cttttttggt
     9181 tttgaaaagc ggccccgtta cggaaataaa gtcgccgcgg tcgagcgccg ctttcaaagt
     9241 tttaaaatcg gaaagattat cttttttaaa aacacactgg attgcgcccg attcgtcggc
     9301 gcaatcgaca aaaagaagat taccctggtc acggatgctc atcacccgcc ccgcgacgga
     9361 aatctttttc ttcgatttca tcagcgcgga gaaacgctcc agcgcttcct ttatcgcaaa
     9421 agaacgcagt gttttcgccg gatacggatc aatgcccgca tcatgcaaaa gatcgagttt
     9481 cctgagccgg tcgttgataa tatcgtcaag catgaaaaat gttccaatgt tccaatgtct
     9541 cgtatctcct cactacaaca acagatcatt acaacatcgc attactctat cttcactatt
     9601 tcgtaaacgc tctttcccga aggcgtcgcg accgtcacgc gctcgcctgc ttcatggttt
     9661 aaaaacgcct ttccgagcgg cgattcgtcg gaaatttttc cttcctccgg ctttgcctcg
     9721 tacgagccga caatggtgta agtaaatgtt ttttctccgg aacccttttt aaccgtaagc
     9781 gtcgaaccta ctttcgccgc gccgccgccg tccttgctga tcgtgatcgc tttcctcagg
     9841 agatcttcaa gctcgaatat ccttgactcg acgctcgcct gttcctcgcg cgcttctgaa
     9901 tattctgaat tttccgaaag gtcgccgtat tctttcgcgc gctgaagacg ctccgcaatc
     9961 tcgatccgtt tttttgtttt gagctcttca agctcccgcg tgaattcctc ccgccgatct
    10021 tttgttaaat aatgcttatt cataatagat atgccgcagc gctccaataa gagagcgtta
    10081 ttttttcatt acaacacccg gaacattaca tattttaatt ttattttata ggacgtacgc
    10141 gctggtgccg ggtacagggc attttccggt tattttccgg gatgtagtcc ggctgcggcg
    10201 atgaaaataa tcggaaaata acgaagggac gggccttgtc cgctgcggtt taagatattt
    10261 tcttatattt ttttcaaatg cgcacgtcct atcaaattac atccgaatcg cgttgctcta
    10321 ttatataccc gttcatccaa agacgccagc ggacagtaag agtttcccgt aacactttta
    10381 gataagccga tgccttgaca tgggatcgcg tgtcattcgc ccaatgcacc ggaatttcct
    10441 tgatacgata gcccatccgt ttcgcaagcg caaggacttc gacgtcgaat ccccatcccg
    10501 ttatccgcga agcgccgaat actttttccg cggcttcttc ggtgaacgcc ttgaacccgc
    10561 attgcgtatc ccaaagtccc ggcaacagta aaatttgaat aacaagattg ccggcctttc
    10621 ccggaatctg ccggtaccac ggctccgccg gatcgagcac ggcgcccttg accgcgcgcg
    10681 atccgattac gacatcgtaa taggtgccgt cggcggaaga tttaccctcc cgatttttat
    10741 aaggcaaaag tctggaagat aaaccgtcgc cggataccaa gccgtcagcg aatctttcaa
    10801 cttttttttc gcctccggtg aaaaaaaacg gcatcatttt ttcgaactga tcgatggacg
    10861 ttgaattatc ggcatcggca aagagccgga tattccccga cgccgcaagc attccctgct
    10921 tcaccgcgcc accctttcct ttattctctt tattgtcgat aagttcaaga tttttcacca
    10981 tctttgccat gccaagaacc gttgccgccg tattgtcttt cgagccgtcg ttcaccacaa
    11041 taatctcgta cggatacgga acgccgctca aacgacggtc catatcaatg agcgttttcg
    11101 gaatgcgtgc ctcttcgtta tatgccggaa taattatgga aaggtaaggt ttcgccatgg
    11161 caatgagttg tattatgaaa atttattttt aaaaccatcc tcacaaacgt aagaatccct
    11221 ctctgtctcc tctgccgtcg tcccgaaaaa caggatcatg cttccgatct tttgtcttta
    11281 ttttaaaaaa gcgccggtca tcggtcggag ccgcgaagac ggcaattttt cttataaaaa
    11341 ctattccgtc aatatgcgcg gtccgttttc cgttatcgcc accgtgtgct cgaaatgagc
    11401 cgtaagcgat ccgtcccgcg cggcataact gccgtccttc agttcccgta ttgcgcccgc
    11461 gcccgcggcg aacatcggct ctatcgcgat aaccatgcct tcgcgcaaca gttcgcccgc
    11521 gccgcggacg ccgaaattgc agacgtaagg atcctcatgg agcgcgcgcc ctatgccatg
    11581 gccggtcaat ccgtcaacga cggaaaaacc gttcttcttc gcgatgcgcg caaccgcgaa
    11641 accgatatcg ccaagcgttt tcccgggttg cgcttctttg atgcccgccg taagcgccgt
    11701 ttccgccgcc gtcataagtt tctttgcctg cccgctgacg ctcccgatgc cgaccgtaag
    11761 cgcggcatca agaaaaaaac ctttatgacg gacgccgcaa tcgagtttga ggacgtcccc
    11821 gtcctgcaat aaataaccgg acggaacgcc atggacaacg acgtcattga ccgaggcgca
    11881 caacgttgca ggatatccgt gcgcggcgcc tgccggacga taatgcaaaa acgccggctt
    11941 tgcgcccgcg cgcaaaatcg ctttgtaagc gaccgcatcg agttctttcg tcgcaacacc
    12001 ggcccgcgcg gcaattttaa gctcccgcag aacgtcgccg agaatccggc cgccttccgc
    12061 cattatgccg atctcctcag gcgtctttaa ggtaatcatt caatttaagg aatatcttgt
    12121 acggcgcggg tgcggcatca agaattctga tgcggtaacc gcgttttttc aaaaatccga
    12181 aaatgggttc cgtgcgttcg cggtattcct taaggcggac cgcaatgact ttcacatcat
    12241 caagcgtccg cttataaaac ggccccgcgc ataccggaca atatttcgca tgcgccgtcg
    12301 gataatattt cgtgaggagc gtatagccgc agatagagca tatcatccgc ttgctgtttc
    12361 tttccacggt tccggttggc ggcaggcgca attcaaaaac aaaaatattt ttctttccat
    12421 agtaccgctc caaaattggg aaaagccctt ccgcttcgta caccgtacgc ggcgaaccgc
    12481 tgaaaacgac gccgaaaccc gctttctcga tccgcttcgc cccgttcgca acctcgcgga
    12541 gcacgaagct cggcgtcata agaataccgt cgtcaaaaag cttccgctca tgcttgataa
    12601 tcttttcttt ttgccgcgcc ggatcatgca cgacgctttc caggaatttt ccgctgtcaa
    12661 aatgcgttac gccgagccgg gcggcaagaa gattagcctg cgttcctttt cccgctcccg
    12721 gcggcccgta taaaatgatg gcaatcttgc gcataaaaat gttggagtgt tgcaatgttc
    12781 tattatttat tcccgtgcca tatttttcat tgcaacattt ataacactac aacactacaa
    12841 cattttttac tattcaatcc cctcgtattc ccttaccgtc aactgcgaat cgacttgccg
    12901 catggtctcg agcgcgacgg cgacaacaat caggatcgcc gtgccgctga ccgtcaatgt
    12961 cgcgacgccg gtgaatcttt ggatcaagct cggaacgatc gcaataaagc ccaggcccgc
    13021 cgcgccccag aacgtgatcc tgttcacgac actgctgaaa aacttttcgg tgttctcgcc
    13081 gggacggatg ccgggaacga atcctccgct ccgctggagg ttcttcgcca cttcttcggg
    13141 attaaacgtg atggaagtat aaaaataaca aaaaacgaaa acaagaagga agtacacgat
    13201 attgtaagcg aactggtttg ccatgaatgc cgtcgcaaga tcattcacct ttacgccgag
    13261 agaggacgaa aaaatagtga ctatctgcgc tataaactgc gggaaaagca gcacggaaat
    13321 cgcgaaaatg agcggaatca tgcccgcctg attcactttg agcggcagat agctttgcga
    13381 cccgccgtac atcttgttcc cgcggacgcg acgcgcatag gcaacgggaa ttttccgctc
    13441 gccctcgttc agatagacaa cgccggcgat aagcagggcg cccgccgcca aaagcgcaag
    13501 ataagcggtc aaaagcccgg gcgtataggt cgcgaatgca gcgccgatgc ccgaaggaag
    13561 cttgcttacg atccccgcaa aaatgataag cgaaatgccg ttgccgatct tctgttcggt
    13621 aatgagctcg cccagccaca gcgcgatgat cgagccgcac gcaatgacga tcacattccg
    13681 gatcatactg aaaggatccg gacgggcaat cacgccctgc gcgataagca gggtgagaaa
    13741 tccatagccc tgcgccaagc cgagcggtac cgcaagcatc cgcgaatagc ggttgaattt
    13801 cgcctgcccc gccgcgcctt cttcgtaata catctctttg aacttcggga aaatgatcgt
    13861 caaaagctgc atgataatcg tcgaggtgat gtacggcccc acgccgagca tcacgatgga
    13921 aagattggaa agcccgccgc ccgaaaagaa attgagaaaa ccgaaaaact ggttcgtgtt
    13981 gaaataattc tgcaggcgcg ccgcgtcgat gcccgggatc gggattgcgg cgaacacgcg
    14041 atacgcgacc aaaagaccgg cgacaataaa aaccttgttc cgcaattctt tgatcttgaa
    14101 aatctgaagg aaacggttca tggagtgttg taatattaca gcgtgctatg ttgtagtgtc
    14161 cgaaatgctg taatgaaaac gctcactaca acaaaaccgt cttcggttga cggaacatta
    14221 caactctata actctatctt cccgccggct ttttctattt ttttcttcgc gctttcggaa
    14281 accgcaatgc cctgcacgga aacgggaaac gcaatagcgc cggttcccag tatctttatc
    14341 gttcctttga atttctttcc aagcagtccc gcggttttca aaaacgcttc cgtaacgata
    14401 acccgatctt tgtcttttgc gcccgatttt gaaccgttcc ggcgtgcaag cgcggatgac
    14461 aatacgccaa gattgagcac ttccgtcttt tcgtttttcg gtttattccg gaagccgcgc
    14521 agtttcggca gccggataat gagatcgcgc tccgccggac ggattctcct gcccgcgcgg
    14581 gatttttgtc cgttctggcc gcgtcccgaa taactgccgc gttttccgcc gcgtccgata
    14641 cgctgggatc ttttttgaat tgaggtgaga gaatgtagtt tcaagtctta attagaat
//
LOCUS       DAIDDT010000018        14156 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_18_length_14156_cov_5.355068, whole genome shotgun sequence.
ACCESSION   DAIDDT010000018 DAIDDT010000000
VERSION     DAIDDT010000018.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14156)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14156)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14156
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18_length_14156_cov_5.355068"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3665 a         3854 c         3444 g         3193 t
ORIGIN      
        1 gagtttcgcg gccttgtatc tcagtcatct tttggttaat caaaatgaaa cttagatttt
       61 cgaggttttc gaccacgctt tacccaaaaa acatattttg gtcaaaaaaa ataaaaaaaa
      121 tcggcattct tggattcgga aaggaagggc aatcggcgta tcgctttctg aaaaaaaacg
      181 gagcgttccg caatgccgaa ttttggatat tgagcagcga taaaaaaaca gaaattccgg
      241 cgggagcgtt tgcgaaactt ggcgatgcct atcttgaaaa tttagctcaa ggccgcttcg
      301 acatcatcgt ccgctctccc ggcgtaaaat atcacgcgcc ggaaatacaa gcggcaaaga
      361 aaggcggtgc ggtcataacg agttcgacga aacttttttt cgcatacgcg cgtcggaaaa
      421 cgaaaaatat tatcggcgtt acgggaacaa aagggaaagg aacgaccgcg acgctcatcg
      481 cgaaaatcct tacgcgcgcc ggaaaaaaag ttttccttgc gggcaatatc ggcgtgccgg
      541 cggtcgatct tatcccgaaa cttacggaca gcgcatgggt cgttctcgaa ctttcaagtt
      601 tccagctggc cgaccttacg gaaagcccgc atatcgccgt ggcgctcatg acaacctcgg
      661 aacaccttga ttggcataaa aataccgcgg actaccggaa cgcgaaagcg aatatcgtca
      721 aatgccaaac cgcgcatgac tttgccgtgc tcgcgcggga ttatgccgca agcgccgctt
      781 atgcgcggct cacaaaagcg cgcgtcttta tgttttcgcg gcgccccctc ccgactttta
      841 ctccgcaaaa ttcgggaggg cgcgcgccgg tcgcaaacgg cacctttgtc gagaacggaa
      901 atttttattt ttccgccgga aaacgaaaag aaaaaatatg cgcaacgaac atattacaaa
      961 tcccgggcga acataattgg gaaaatgccg gcgcggcgat cacggtcgca aaaattctcc
     1021 acatcccgaa cggcatcatc aaaaaaacgc tctcaaattt caaaggactg gcgcaccggc
     1081 tcgaattcgt cgcagaaaaa aacggcgtcc gctactataa cgattcctat gcaacgacgc
     1141 ccgaaacaag catcgccgcc atcacggctt tcaaagaacc gaaaatcctt atccttggcg
     1201 gttcctcgaa aggaagcgat ttcacgaaac tcggaaaaat aatttcgaag tcaaaaaacg
     1261 tgaaagcgat catcggtatc ggtgccgagt ggccgcgcat caaagcccgc atccgcaacc
     1321 cgcgcataca gctcgttgaa ggatgtaaaa atatggaatc aatcgtccgc gtcgcggcgg
     1381 aagtcgcgga gcccggcgac gccgtcctgc tttcccccgc ctgcgcatcg ttcggaatgt
     1441 tcaagaatta cgacgaccgg ggcgaacaat tcaaaactgc cgtcaaaaat ttgaaagaag
     1501 acccaaaaaa gggtaggatt cccgtatggc atcgccgata aacaaaaccg agctcgcgca
     1561 tcttgcgaag cttgcccgca tcgaactcgc tcccgacgaa gaaattaaac ttctgaaaga
     1621 cctcgccgag atcattgatt attttaagga acttgaggcg cttgatacgt cgcggatcgt
     1681 tcccatgacc ggcggtaccg atctcaaaaa cgtttttcgc ggagacgacg aacgcgagaa
     1741 caccgatcgc ggcgcgggcg cggagctttt tccggaaacc gaaaacggtt ttttggaagt
     1801 gccgcccgtg ttcgaataaa aaagagaatg ctaatctcta aagcgcgaat cctcgaccag
     1861 gtcgaggacg acggaaggag ataaaaaata taaaaaatga aagcattgca taatctcaca
     1921 atcaaaaaat ttcacgacgg gcttttaaat aaagaattct cggcgcttga agcgacgcag
     1981 gcttttttcg aacatatcga aaacaaagat cctgaaataa acgcttattt aagcttggcg
     2041 gaaaaatcgg ccgcgcggga agcggaagcg gcggatatcg cgctcgcaaa aggagaagca
     2101 atcggcgatc tcgcgggcgt gccgctcgcg ataaaagaca atatcctcgt gaaagggctt
     2161 ccggcaaccg cggcgtcaaa aatgctcgag gattacgtcg caagctatga tgccggtgtc
     2221 attaaaaaat taaaaaatca gcgcgccgtt tttctcggca aaacaaatat ggacgagttc
     2281 gccatggggt catccaccga aaactcggcg ttcaaaaaaa cgaaaaatcc gcatgatacc
     2341 gcgcgcgtgc cgggcggctc atcgggcggc tccgctgccg ccgtcgcggc gcacatggcc
     2401 gtcgcggcgc tgggcaccga taccggcgga tcgatccgcg aaccggcatc gttctgcggc
     2461 gttgtcggat tgaagccgac ctatggcgcg gtctcgcgcg cgggcgtaat cgcgatggcg
     2521 tcgagcctgg accaggtggg acctctcgcg aaaaccgcgg aggatgccgc gatccttttt
     2581 aaggcaattg ccggacgcga tcccatggat gcgacgagcg tcgaaaaaaa ctatttcaat
     2641 aacgaagcgc tctcgaatcc aaagctggag gatattcgaa aattatccat agggcttccc
     2701 gaagaatatt ttattccggg aacaagcaag gacgtggcgg acgccatcga aaccgccatt
     2761 gaaaaattga aaggactggg gctacggttc aaaaagatct ctttgccgca caccaaatac
     2821 gcgctttcat gctattacat catcatgacg gcggaagtga gctccaatct cgcgcggttc
     2881 gacggcatgc gatacgggag aatacggaac gcggagcgcg gagcgcaaaa attatccgat
     2941 ctttatctga aacagcgggg cgccggtttc ggagccgaag cgaagcggcg gagcatcctc
     3001 ggaacattcg tcctttcttc cggttattat gacgcctatt acgaaaaagc gcagcgcgcg
     3061 cgggcgctca tcaaaaacga cttcgacgaa gcattcaacg atgtcgacgt catcctctcc
     3121 ccggtcgcgc cgacgccggc attcatgatc ggcgaaaaaa taagcgaccc gctctcgatg
     3181 tatctttccg atattttcac gatccccgcg aacctcgcgg gtcttccggc gttgtcaatc
     3241 ccggcgggat cagaattgag aatgcaaaat ttagaatacg gaaaaaaaca tcaagcgaca
     3301 aaacttcccg tcgggttcca gcttatcgga aaaaaatggc gcgaagaaga cattctcggc
     3361 atcggacaat tttatgaaca actggaaaat taaaatatga cgaacacatt tgaaaaaaaa
     3421 tcataagaaa ttttctcttt gtcatcgtcc gtgaaaacgg acaacccgcg cttcccgtct
     3481 tttcttctta ttttcaaagc atgggttgtc cggccatgcc ggacaatgac agggggaaaa
     3541 tgccccgtac ccggcgcacg tgcgtacgtc ctataaaaat gaactctcaa aatcaaacca
     3601 tcaacataat ccggagccaa agcgcggcag ccatccaaaa aacgcacctg ctcgccatgg
     3661 ccgcttccgg tatttatata tcaaacctta cgcttcttca ggcgggaagc gccattattt
     3721 ccgcgatcct catcggatgc gcaatctatt tcatcataaa aacggactgg ctcaaaacgc
     3781 gcgtgaaccg gatacaggac gttgtcctta aaaccgacat ccccaaaaaa gaagctcaaa
     3841 aaacatgggc ggatatccag aagcattttt tcgcgggaga cgaaaacgat ctgaaaatcg
     3901 ccatcctcga agcggataag gttcttgacg acgcgctccg tagcgccggc atcaccggcg
     3961 ctacgttagg cgatcgcctg aagaaaataa aaccgtcgca gattcaggac atcgacgcga
     4021 tctggcaggc gcataaactg aggaatcaga tcgcgcatga aaacgatttc aaactgaagc
     4081 gcgaccttgc cgaacgggcg ctcggtgttt acgaaaaaac gcttcgcgat ctcaaagtgc
     4141 tggattaata atccgcgcgc tgcaatctaa gaaaccgaaa aaaataccaa cctattcaaa
     4201 actcctcgtc agaaaacaaa acagcgcctt ctggcgctat tttgttttaa ttgaataaca
     4261 gatatcgatc ctatattacg gcttcgttgt actcgaagca gtcgatgaaa cattttgggt
     4321 caaagcggaa ccgttgacgg cagtgaccgt aataagggcg gatccctgaa gataggttcc
     4381 cgttacctga accgttgttc cgataagaga agcaagcgcc gcgcggacgt tcgcatcgct
     4441 tgaatttttc acggctactt ttacgccata ggaagtcgtg ataacgtagg atgatctcgc
     4501 gccgttctga agcacgccgc ttatcgtcgc cgtgccggtc gttggcaccg aagccggcga
     4561 ggaagaaccc tgaacgcccg tcacaggaac agcaaggaaa gcaaggtttg tcagcatatc
     4621 cgcgctctcc gccgtaagcg acgccacctt atttttcagg tccgcattcg aggcggtaag
     4681 tgtctggact tgcgcagtaa gaccggatac ctgggacgcc gcgccggcgt tctgcccgct
     4741 tacttttgcg gagagcgcgc tattctggaa atacaagaaa ccggaaagtg cggcgagacc
     4801 cacgatgatc accgcctcta taatatccgc aattttccaa ccgcttatct tcggaggata
     4861 ttctttggcg ccggacggca ttttcatttt ttctatttta ttatctcccg ccgaagctgt
     4921 ttttttaccg aaaaaatttt ttgccggaga ttgcggcgag ccggtggcca taccggccgc
     4981 gcgttccggc tgcgccggag cgctttgcac gtcgctcacg atatccaacg gagaaaacga
     5041 cgtaagcgga gacgacggct ttgacgtaac cgcgcctgac ggcatggttt gcgccgcagc
     5101 cagcatggac ggtttctgcg tcatcggcgc ggcgggttgc gccgttgccg cgggctgagc
     5161 tttgtcgctt tcaacttttt tcaacgtatc ttcgacgtca atcgccgacc accccacgcc
     5221 cgtcaacgcc gactttaccg catcgcgcga aacgccaagt ttcatctgcg aggaaacata
     5281 ttccacaaga gaatcttgaa tcataatgaa tgatagctgc tatttttatt tttaaccaca
     5341 tagcaacgat gacctttatt ggtttgtatg gttttatttt accttttccg gaacaaaagc
     5401 aacagcagga gaaatccaca ggaacgaaac actatgcccg cctattaaaa gaacggcatt
     5461 gtttgcatcc gctcgccaat aataaaagaa atcaacccga agcgggcggc tcttttcttc
     5521 tgtaaagtac gaacctattt taccaaaaat ccatgaattt tgacatcagt ttaccccttc
     5581 aaatacccct tcaaattacc tgcgatgaat tgagggggta tttgaagggg taaatccggc
     5641 ggctaaagcc tcggattttt ggtaaaatag gttcgtactg cggatattca ctgcgaccaa
     5701 aacaaaaaaa ggatgattct gaatcatcct ttttttgttt tggtcgggcc gccgggaatc
     5761 gaacccggtc tttacgcacc cgaagcgcac gtactaccgg tatactacgg cccgttcaat
     5821 ttaaaacccg tctcaaaagc tgcttttcaa gcaggccgac ggagcctgct tttgagacgg
     5881 gttctatgcc agttcattta accttacttc ggacgaaaac tcaaccggta attccttacg
     5941 attctttttc cgttcttggc ggcggcgaat ataaagattt caaaagaaat atattttcat
     6001 tcaaacaaac cggaataggg catcgtcatt ttccaaaaaa ataaatcagg ccagaaaaat
     6061 cttcgacgtc atttttaaac gcgtcgaatt ccggcctgcg aacttcgata ccgttaatgg
     6121 cgtcaaggag gcgctctccg attttgcgaa aatcaacgtg caggtcacgc ctgacatcct
     6181 gaaaacccgc ctgcatagcg cgccgtatct cttgaaagcc gtcctacatg cgaagctcag
     6241 aatcagtgaa tgcaatattc gtcgttcgcg caagttcatc aatcgtcatt ttgtcgtcaa
     6301 gtttcttttt gctcgtgatt ctttaatgtt gtttttcatt tgccaaaaat caaatccgtc
     6361 aaaatttcgc cgtttgcggg ctgccgaggc cggtcacata atcgtaccgc ttgcgggcgg
     6421 cgcaataata tttacagtcg ccgttggatc cgctcgtaat atcgcgaaag aacgagcggg
     6481 cgcctgccga cgccttatct ttatagatcg cggcgagcgc aacggaatgg ccgagcgatt
     6541 ggattgccgc ccattgcggc gcgccggcgc tcgtgccgcc gacctcatac catccgttct
     6601 tcgacgaacc ggtggtttta taaaccggat atcccgattg cggatcggca ttgtacgata
     6661 cgtccggtat cgcccgtttg ccgccggctt tcgaaatttt gtatgacttt tgataacccg
     6721 gttcggtttc gtacgcgctc acgccgccgc cgctccccgg ccacgccgac tcggcaacga
     6781 atgttcccct gcttgaaaaa tcgagggacg tgccgccgac gcccacaaca ttcggcgatg
     6841 acgccggcca gcttgcgccc cagccgtcat cccctgacga tgcgaagaat gccgcacccg
     6901 aaacgctcgt gaagtgcgca tcgagaccgg tctcgtcaga aaattcggcg ccgccccagc
     6961 tcatcgagac cgcgacgacg tccttccgct tcgccgcgta gtcgatcgcg cccaaaaggt
     7021 tcgcgccgct ttcggttttc gcctcgacca gcaatatttt cgccttcggc gcgatggcgt
     7081 gcgcccactc gacatcaagc gacgtttcaa gcgcccatcc gctgtcggcc ttcgtcgatt
     7141 tcgccataat gtgtttttca aggcatttgt tttttactgt gcaggcagga agcttgaacg
     7201 cggcgtcgaa aacggcaaga tctttttcga tggcggcgtc gttatacgca tcgatgatgg
     7261 cgatcgtccc tttaccgccc gttgcgggca gcttataaat cttttttatt tcggcgggcg
     7321 tcacgcctgc aggcgatgcc ctcgccgacc ggaaaatatg caccggcggc cttcctttaa
     7381 aatcggaaaa tcgataggcc gcatcggccg tattattcga cccgcccgcc aaaagcaccg
     7441 ccgcgaaagc ggccgcggaa atcgcagcaa accattcaga atttttcttt aaaaaatcca
     7501 ggaaagcaaa cgccgcggaa aaaattttgt ttttcataat gccatcctac cgcttatggc
     7561 attaaaatta tcttaatagg attttcttta aaaccgcgag tgccgcatca atctcttttt
     7621 tcgccgtcgg gcggccgaaa ctgaaacgga cgctttcttt cgcgcggttc tttgatgacg
     7681 aagaaccgaa atcggaacca tggctacggc cgagcgccat gataacatgc gacggatcaa
     7741 gcgcgcggct ccggcacgcg gaaccggacg atgcggcgat gccctgcata tccaatcgcg
     7801 taagaatatc ctgcgcatca cgcccgggaa aataaacatt caggatgttc ggcaaggcgg
     7861 aaggcaatga acgcgcaacg gcggacgccc gcggcgcgat accgttcacc gccgctttcg
     7921 gataaatttt ttcgatccct ttccaaaaat aatcgcgcag gcgtgccatg cgttccgatt
     7981 ctttttcgcg cgccgctgtc gccagttcaa ccgccttcgc gaacccgacg attgaaggaa
     8041 cgttttcagt ccccgaacgc agaccgaatt cctgaccgcc gccggtgata atagaattga
     8101 gaatagagaa ttcagaattc agcggcgtgt tgttttgttt tccttttgaa tcccgagtcc
     8161 ctgcttttcc cttttctata ttctgaattc tatattctgt attcttcttc gtgtacaggc
     8221 acccggttcc tttcgggccg taaattttat gcgccgagag cgtcatcata tcaacgccga
     8281 gctttgccgc gtcacaatcc aaaaattgga acgcctgcac ggcgtcggta tggaagagtg
     8341 gaaaaaaatg tgccgcggca gcatagcgtt cttttttaaa atcatcgatt atcttccgga
     8401 tttcaaaaat cggctggacc gtgccgatct cgttgttcgc atacatcacg gaaaccaaga
     8461 tcgtgcggtc gtcgagcgac gctctcagcg cgcggagatc gatcatccca tttttatcga
     8521 ccggcagata tatcacttca acgccttcct tttcaaattc gcgcgcggtt tcgaggatgg
     8581 attcgtgctc gacggacaat aggatcactt ttgcgcgata atcatccgaa aacacatccg
     8641 gccgacactc gcgatctttt tcgaacaatg ccgacgcttc tcgcattttt cgcatcgcat
     8701 tgaccacgcc gcgcagcgca agattattcg cctcggtcgc cgaaccggta aaaatgatgt
     8761 cgcggaattc cgcgccgatc acgtgcgcga ttttctcgcg cgaagcgtcc accgccgcga
     8821 tcgcctcctg cccgaacgaa tgcaccgatc ccggattgcc gaaacgctcc gaaaaatacg
     8881 gccgcatcgc ggcaaacacc gacggatcaa ccggcgtcga agcggcgtaa tcgaggtaaa
     8941 ttcttttttt gcgagcgttc atgataatga tcataacgca tggcgctctt ttttgtcatc
     9001 tatgtcatcc atgaaaccgc ttctcgcctt cccgaaggaa aaggagggag cctgtggata
     9061 agtcgggggg aaaaaggaag gagaaagata ttctataata ctttactgcc cccgctgtcg
     9121 tcctcgcgga tgtcgaaatg aaaaacctat aataacgtcc cctgtttcgg aaacacaatc
     9181 ttctttgctt caccagcccc cttgtcggga acccaaagcg catgttcgtt ccgaaaaaga
     9241 tcataaagtc cctttgtgag acgcacgtcg ttcaggcaat aatctttcag ttcgtccatc
     9301 ttcccctctt tatagagagc gatcgcctcc catccgtgat gggttttttt tacgccgaga
     9361 ttcgcctcgg caagccgatc caaactgacc ctgtttcccg tcgccatttc aatctcatcc
     9421 aaaagatcaa cgcggtcttt tttccagaga tccacttttt caaaccttcc cgacgcgggc
     9481 gatgcaggcg tctcgggacg gaaacgttca aagtagagat tcaggacggg aatgtcatac
     9541 cggttactgc tgaaaccgac gatccggttc gcgccgcgga aaagttccat aagtccgccc
     9601 atttggtttt cttcaaacgt gaaatactta tcctgcgcgt acgaatacgc gccgacgacg
     9661 gaaattttga gcgccgcgaa attgtcccgc cccacgcccg ggtcggtgaa aaaattctgc
     9721 gtttcaatgt caaatacgac ggtgtccatt ggaaaaagta aaatgtgaaa atgtctgtcg
     9781 tcctcgccct atggagtggc ggcttcgctt attccacggg gcgaggattc atgcttaaga
     9841 aatagcgatt agttagtagg aagagagaac gattagtgat taaagaaaaa gatgggaagc
     9901 gtggatcctc ctcttcagga ggacgacaga gggaatagat cccctccttc gggaggacga
     9961 cggaaaaaaa gcaaaaatag caaaaaaaat taatgctcca atattttttt aataatttcc
    10021 ttcgtctctt ccggattgcg gacggcgatg caatcgacgc ccgtccggcg cgcggcggaa
    10081 tcattgccgc cggaatagag cgcgtcgccc acgaaaacca tatcccgcac cggcactttg
    10141 agctgccgct cgattgcacg gatgccgtac gccttgtcga tccctttgcg ggtgacatcg
    10201 atggtcgtca aaccgccgcg atgcacttcg aactccgtca gccgcttcgc caaaatgtcc
    10261 gccactttca cgcgcatcgg ctcgtatttc tttttccatt cctctttgcg ccgcacgcct
    10321 tctttctcgc cgcaatacgc gacgatatcc tgcccgagcg gcgaaaaaga cacttgcgtg
    10381 ccgcgatcct cgattgtttt tccgtacgtt ttttccggca gcacatactt cgtttctttc
    10441 aaaacatcgc ggaacgcttt tatgattttc gcccgttccc gcacggacag cgtcaaagta
    10501 tacacatttt tccagccgcc gtcataacgg taaaacgtgg tcgaggttgt gggaaacaga
    10561 aacatgtttt tcaaaagcgc cgcgggacat tttttgaacg gcgcaagcag ctgtttcttg
    10621 aaaagaccgt atttcccgcc gccgatcacg gcgacctttt ttaccgccag cagccggaca
    10681 agcaaccggc tcattgcggg atccataggc gctttgctcg gcgtcaacgt tccgtccatg
    10741 tcgaacacaa tgagcttctt ttttttcaat aacgcgggga cccggcggat cgaataaagt
    10801 tttttcattg ttgttattat accacttaaa attttcttcg ttgctgtcat cgtccgctct
    10861 cgattgtggt caagggcagg ctccgacgga cgacccgtgc tttaaaaaat taagaaaaaa
    10921 gacccggagt atggattccc tccttcgggg gaacgacgca ataagcattg cgccggctgg
    10981 gaaacatgaa tccccgccaa agtcgagtac gacgcaataa aaattgcgcc gattttgtct
    11041 cccgcggaaa atagagtaca atggattgcg ttatggcaaa aaataaaaag aaaaagaacg
    11101 ggaaaaatgg cggcgcggaa aatgaaaatg gcggcgcggg acggcgcgaa aaaacatacg
    11161 aaaaacatca gcttcatccc gacacgaaaa aaagcatctg ggtgataagc ttcatcggcg
    11221 ccgcagccgt gctcgcgctc gcggggttcg cctccgccgg tccgctgggc catttcatct
    11281 ataccatcct cactacgctc ctcggctggg gctatttcat cctgcccgtt gcgcttcttt
    11341 tcgcggcaac cgtcctgctc gtttccgaac ggcgaaaact gctcggcacc accatgatcg
    11401 gcgccgcgct gttcgtcctt tccgtcctcg gcctcatcga actttttaat ccgacgaaag
    11461 gcggctggct cggccttgtg ctcgggtcgc tccgcattcc tttcggccag attgcggcgg
    11521 tcatcatcaa tgccctgatc tttattatcg caatcctgat caccgcaaac gtcccgctcg
    11581 cgacatggtt cagaaaaaaa gaaaaagaaa acgatgaaaa cgggaaaccg cttgtcgtta
    11641 cgggcgccga agaaaaaaca ataactgaaa aaaagaaaga aagaaaagaa aaaacgccgg
    11701 aagaaaacgc cggaaaagag gttgatgagc cgcttgtcgt ggaacataaa gaattgacgc
    11761 ccgtaaaagg gaaaaaatcg aaaccgccgg tctttgcgaa ttacgttccg ccgccgctct
    11821 cgcttttgag aaccgaattg ggaaaaccga ccacgggcga cctccttgcg aacgctaaca
    11881 tcatcaagcg cacgctcgaa agtttcggcg tcgtcgtcga aatgggcgaa atcaacgtcg
    11941 gccccgctgt cacgcgttac acgttgaaac ctgcggaagg catgaaactc tcgcgcatca
    12001 ccgcattgag ttccgatctt tcgctttcgc tcgcggcgca tccgatacgg attgaagcgc
    12061 ccatcccggg aaaatcgctt gtcggcatcg aggttccgaa taaaacggcg gcattggtgc
    12121 gcctcggcag tttgatgcac tatcccgact tccaaaaaaa tgatcttgga tttgtgcttg
    12181 gacgcgatgt ttcgggcgaa ccgacgttcg gcaatatcga aaagatgccg cacctgctca
    12241 ttgccggcgc caccggttcg gggaaatcga tcttcgtcca ctccattatc atttcccttc
    12301 tttataaaaa ctcgccacag acgatgaagc tcgtcctcat cgatccgaaa cgcgtcgagc
    12361 tttccgttta cgaaggtatt ccgcatcttg tgagtcccgt cgttaccgaa aacaaaaagg
    12421 ccgccggcgt tttccgctgg gcgctcgcgg aaatggaccg gcgctatgac acactccaac
    12481 aggccggctg ccgcgatatc cgcagctata acggaagcca tgccgcagat ccgctcccct
    12541 acatcatcat cgtcgtcgac gaactcgccg acctcatggc aaccttcgga cgcgaagtcg
    12601 aaggatctat cgtccgcctc acgcaaatgg cgcgcgcggt cggcattcat cttattcttt
    12661 ccacccaacg gccgtcggtc gaggttatca cgggactcat taaggcgaac atcacttcgc
    12721 gcatcgcgct ccaggttgcg agccagatcg actcgcgcac catcatcgat accgccggag
    12781 cggaaaaact gctggggcac ggcgacctgc ttttcctttc tgcggaactt tcgaaaccga
    12841 aacgcatcca gggatccctc atcaccgaag acgaaataaa aagcgtggca gagttcatac
    12901 gcgaacacaa caaagctgtt ccgcgggaag cggaaagcga gataaatttc gaagccacgg
    12961 gtgccactat gaccggcaaa ccggaacgtt tcgacgaata tctcgaggac gaagacagtg
    13021 acgagctgct tgacgaagca atcgcggtcg tcgccgaagc aaagaaagca tccgcgtcgc
    13081 tcctccagcg ccgtctcaaa gtcggttacg cgcgcgcggc gcggcttctc gacctcatgg
    13141 aatccgccgg tctcatcggt cccggcgacg gcgcaaaacc gcgggaggta aacatcgaga
    13201 aaatagaaga aaggttgtcg tgacgaagcg acaagccgtc atccgcgaag gcggacgatc
    13261 tacacttcca atcttttcct tctgccttct acttttttaa ggcgtcgtcc gcgtcgcggc
    13321 gaggacgacg gagggaaata cagataccaa aatctcccga tttcctctat aatcacctta
    13381 tgaaccacga agacatccct tttgaaaaat tttttaccga ccggctgaaa cagaagggaa
    13441 tgagcataaa aaaactcgcc gagacgaccg gcatcgcccg cagccatatc gagaacatcg
    13501 caagcggtaa tttcaaagat atgccctcgg cgccgtattt ccgcggctat cttatccggc
    13561 tcggcaagat tcttgatttc gacggcgatg agcggtggga agaaatcaaa aaggagggta
    13621 tgctgaaaaa atccgggccg aaggatatgc ttcccaaaaa ccggtttatc aaaaaacatc
    13681 cgtcgaaatt catctgggcg ggcgccgttt ttgtcatcgc aatcgtctat attattttca
    13741 ggtttgcgag aatagcggga atgccgacgc ttacattaat cttccccgct gaaaatcccg
    13801 ctacggtatc atcttctgaa attaccctgc aaggaaccgt aaccaatgcc gattcgcttt
    13861 ccataggcgg cgaaacggtg ccgatcgcgc aggatggcac atggcaaaaa aacgttcttc
    13921 ttttccccca aaatccgaat acttttcaga tcaccgcgaa aaaattcctt ggcggcgaaa
    13981 cggatatcgt agagcagata ttctatgaac cggccacagg aacattctcg accggcacaa
    14041 catcaacatc cataaacagc tcaggaacaa tacagggaat acggtaaaaa taatccggta
    14101 tttaaagatc tgcttttata tcccgcgctc tcttttcttt tgtagtcgtc cggcct
//
LOCUS       DAIDDT010000019        13635 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_19_length_13635_cov_5.633943, whole genome shotgun sequence.
ACCESSION   DAIDDT010000019 DAIDDT010000000
VERSION     DAIDDT010000019.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13635)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13635)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13635
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19_length_13635_cov_5.633943"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3627 a         3144 c         3641 g         3223 t
ORIGIN      
        1 ctaattaaaa ccatgcgtat ttttggagtt accataccgg ataataaaag gatagaaatt
       61 tcgcttacgt atgtttacgg catagggccg acgttgagca aaaaagtgct tgcggccgcg
      121 aatgtggaca ttgataagcg cgccaaggaa cttaccgctg acgagatctt gcgggtgcag
      181 aatttcatcg agaaaaattt tccggtggaa ggggaattgc ggcaggtcat caagcgcaat
      241 atcggacgcc ttaaggaatt acaggcgtat cgcggcatac ggcatatgcg gcgattgccg
      301 gtacgggggc agcggacgcg tacgaactcg cgcacggtgc gcggaaacat cagaaaaacg
      361 gccggcagcg gaaagagaaa agtggatctt aaataatagg atagagaata tagaaaaaaa
      421 tcgattcacg ttctcaattc catattccat attctaaaat actaaattct aattaagaca
      481 tgggaaagaa aaaagtaaca actcaatctt cggaagaggc ggtaaaagaa aaggctgcgc
      541 cgaaatcagc ggccggcaag aagtcgagaa aagtgagcga aggaagggtg tacgtcaacg
      601 cttcctataa taatacggtc ataacggtga cggacaacac gggcaacgtt ttggcgtggg
      661 gatcggcggg atcgctcggg ttttccggtc cgaagaaagc aaccccgttt gcttcatcga
      721 aagtgatcgc ggctattgcg gaaaaaataa aagcgacggg gccggacacg gtttcggtta
      781 tcgtgaaagg agtgggcagc ggaagggatt ccgcgatccg gtcgctcatc gcccatggat
      841 tcaccattct ttccataaaa gacgcgacgc cgatcccgca taacgggccg cgtcccgcaa
      901 aaacccgcag aatttgattt tccccccgct attcgctatg ctttatcctt taataatcat
      961 ttaattttat tttcatgctt cgcggaccaa aagaaaaaaa agaacgggca ttgggcgtgc
     1021 gccttggatt caagggcgag cgcgctttgt cgccgaaatc cgcccttgtg cggaagccgt
     1081 accgtccggg cgtgcacgga ccgaaatcgc gtccgcgggc gatgtcggag ttcggccttg
     1141 agattaagga gaaaaataag tttaagctta tgtacggcgt tgacgagaaa aatatgaaac
     1201 ggctttataa aatggcgcag gccgtcaaag gcgccgccgg catgaagctg atagaatttc
     1261 ttgaacggcg gctcgataat gtcgtgttcc gccttggttt tgcgccttcc cgcgcggcgg
     1321 cgcatcagct tgtattgcat ggtcatatca cagtgaacgg caaaaaagtg aactcgcccg
     1381 gatttatggt gaaggtcggc gacattatcg gagtgaaaag cgagcttacg ggcgcgcttg
     1441 cgaaacaaaa agaatcgctg gagcagtacg atgcgccggc gtggcttacg ctccagcccg
     1501 ggaaaatgga aggcaaggtt ttgtcggttc ccaaagatac cgaggtgcct ttcgaaataa
     1561 atttattggt cgaatcattc agcaaatgat tgaacggttt tttgttcaat catccccgcg
     1621 gaggcgagga tccctatgcc aataagagag tgtggcaaaa aataattaac aaaatataaa
     1681 taatatggaa ttcactcatg tcagttctgc ggtttttata aaaacagttt ccgaagacga
     1741 aaaaaacggc gtttttgaga tcggcgggct tttcgcgggg tacggattga cggtgggaaa
     1801 cgctttgcgt cgcgcgcttt tgtcgtcgct tccgggcgcg gcggttacgg agatcaaagt
     1861 gaagaatgcg ccgcatgaat tttcgaccat cccgggcgtg caagaggata ttgtggaaat
     1921 tttactcaac tttaagaaac tccgtttccg tttgcatacc gacgagccgc aggtgttgac
     1981 gcttaaaatg aaaggcgacc acgaggttac gggcggcgac atagagctta attccgaagt
     2041 cgagcttatg aaccccggcg aaattttggc gcattgcacc gcaaaggatg ccgtgcttga
     2101 tattgaggtc aaggttgaac gcgggctcgg ctacgtgccc gttgaggcgc gtaaagcgga
     2161 gggcggacat ctttccatcg gcaccatcgc cattgacgcg atttttacgc cggtgcaaag
     2221 cgtaaattat acagtcgagg atatgcgcgt gggcgatcgg acggattata accgcctgcg
     2281 gattacggtc acaaccgacg gcacgatcag cccttcttcg gcgctccata aggccgcgaa
     2341 tattttgaaa gaccatttcg ataaagtttc cacggtggag gtgcaggagt tcgaatcgtc
     2401 ggagacggaa tcggaaaaac ccgccgtggc gaaaaaaatg aaaaagacga aaaaataaaa
     2461 tagcgtaaat agaatataga aatcagaata gagaatagta ggctgaaaaa gaattaacct
     2521 gcattttaaa ttctataata ctgatttttt aaaaaacaat gagacactta gcaaaaggta
     2581 aaaaattcaa tcgtttgcgg ggagaccgca tttcttttat gcggaatctt gcgaacgacg
     2641 tcatccgagc ggggagcgtc gaaacgaccg aagtccgcgc gaaagcgatc cggccggtcg
     2701 tggaaaagct cgttacgctt gcaaagaggc agacactggc aagccggagg ctgatcgtac
     2761 agcgcgtgca taacgcaaag attgcgcaaa agctttacga agagcttggt ccgcgctatg
     2821 ccgaacggaa aggcggttat cttcgcattg caaagctcca aaaatcgcgc aaaagggacg
     2881 gaaccaaaat ggcgaggata gagttcgtat gacgaattga tcgaatgttc gaatgttgta
     2941 atgtgcatta cgctacaaca ccaaaacatt ttaaacacta taacttttca acatggatta
     3001 tcatattgac gcaaaaaata aagtattggg ccgcacggca tcggaaatat cccttatttt
     3061 acagggcaaa aaaagcgcgt cgtatgcgcc gaaccgcgtt tccgacgata aggttttcgt
     3121 ttcgaattgc ggcggcatcg tcgttacggg caacaaagag atcggcaagg tgtattatca
     3181 tcacacgggg tatgtcggcc atttgaagga gctgacgtat aaacagaaat tcgcgaaaga
     3241 tccgaaggcg gtgctccgcc aggcggtgcg taagatgctt ccgaggaatt ttttgaccgc
     3301 ccgccgcatg aagaacctta tttttgtcga agacgcaccg actgataaag taaaaaaatc
     3361 ataatatttt tcaggaaaga ataacaccaa cctttaattt ataatcatgg cacttgaaac
     3421 agtaaaaaaa gaaaaaaaga ttcctgcggc gcggatagcc cgcgagccga aaccggctcc
     3481 tgcggtaaaa acgccgcatg ccgccgttca cgctatagcg ggcgcggaca agtccgtagt
     3541 aaaaaaagcg cgctataccg aagcggtggg acgccggaag acggcgattg cgagggtgag
     3601 aatttttccc ggcgacggga ccatgaccgt gaacgataaa gacgtgaaga aatatttcgt
     3661 ttcggcgcgt cagtgggtta ccgcacgcgc gccttttacc gacctgaaga tcgtaacgtt
     3721 tgatgcgagc gtccatgtcg tcggcggcgg tatttgcgcg caagcggaag cgatccgtat
     3781 gggtgtcgcc cgcgcgcttg tcgaaaaaga tcatgaatgg aaaaaacgcc tgcgcaccgt
     3841 cggtttcatg acccgcgatt cccgcatggt ggaacgcaag aaatacggcc tgaaaaaagc
     3901 ccgcagagct ccccagtggg ctaaaaggta aagcaccacg aattctgcat atagtcaaac
     3961 agctccgaga atatcggagc tgtttttacg catctgttca gaaattgaat atatgatttt
     4021 tctgcgaagg aggaaacttt gtcaaaaata attttgtgtg aaaaagatat gcgtgcagaa
     4081 attggataga acgctatcaa tggattagaa atataaatga aaaacagggc ataggagcgg
     4141 cctttgcggg aaatagcgtc agattcatcg atgcgccgaa gcaaaccttg caaccacacg
     4201 gggccacgtg aggagattcg gagccggacg ggattgtttt cccaaaggaa aagagcaaaa
     4261 aaggggcgat aaaactgaca cgaattgtgt ctaaaaaata ccaaactctt gccgcgcatg
     4321 cgacaggagc tctaccggcg ttggattaag ggcgacaccg ttgccgatct gtcgcgccat
     4381 tacgatattt cccgtcctac cgtgtaccgg acgatcaaga aggcgaggat ggacatcttc
     4441 atcaatctgc cttcgatgaa ctaccggtat cggaagatcg aatacggctt gaaaaagttg
     4501 agtaagatag aaaagatact ggcgaaaaag cttgctagga gagaacaccg tctcaagcga
     4561 tacgagaagg ccgctcccgg cgagatggtt catttcgaca cgaagaaact acccgtcatg
     4621 tacgaggaag cgatcacgca gcccaaagaa tggcttcatg tggcgatcga tgatttctcg
     4681 cggatgcttt tcgccgacat cctccctgac aaaacgggat attcttcagc catctttctc
     4741 gaggaaacga tccatgcgat gccgtttcag atcgacgtgg catattccga caacggatcg
     4801 gaataccgcg gcagacccga ccatcccttc attgcagggc ttgcgaggca tcatatcaat
     4861 cagcagcata cgaaaccccg ccatccgcag acgaacggca aggcggaacg ggtcatcaag
     4921 accatcatga ccgagtgttt tgctcgcagc ggcagaacct tcgcttcccg tgatcatcgc
     4981 aggaaatttc tctacgcttt tgtgcattga tacaatacca ccagacctca tgaacccctg
     5041 ggcggcgtgc cgccgctcca gaggatcgaa gcctatcttg ccagagtcaa aaatgacgca
     5101 aaatgaacta acccgctaag aatgtacacg gccgcttgac aaatagatag catatgttaa
     5161 catatagaca gtttcttgaa aacttaattg tgtttgccat aacagagagt gacgcttcct
     5221 ttgatgaggg tctttgtctg ttatggcaaa ccgggaatcg tcccggagcc ctctgacaaa
     5281 gaaaggagga ggttttatga agaaaataat agcaatagta ttattagcag taataatgtt
     5341 tttgtattca ttgccggcgt tcggtggaca aactcccacg ggcaatacga ttaccatagg
     5401 aacgccgcag ggaagcgtaa taatgaagaa tttttataag accgcagcga tagtttctcc
     5461 cggatcaggc gctctcattg tcgataatcc tgcctatgat atcagttatt ataccggcga
     5521 tagttcattt ataatttccc tgaaacaacc tcttaccatg ccgacgcgcg cggcggcatc
     5581 agaggctttt atccaggtat tggggattag cgagcaggat gcgtgcaagc tgaaagtaat
     5641 actcggagtg ccgataagct tcgatccgaa aaatgcaggg ataaattttg gattgagctt
     5701 ttgcggaaac tccgccttac cccaataaaa gacgatattc ttaatagggt tctcttgaaa
     5761 ttaagttgaa acgcggtaaa atagaaaatg tgaaagcaat tattaaacgt cataccttaa
     5821 tcaaagaggt aaagatgtat gcagatagta ttgatggcaa agccatctac tttacggatt
     5881 tcgtatctta tctcataaag aaaattaccg accataaacg ggtatatccc ctttgggata
     5941 aaaaagattt agaccgtgat ataacaaata ccgcgcttga ttgccgcgag aaatatataa
     6001 taattatgga ttttcattat ggaactatta attaaaggag aggtcataat aattttgcca
     6061 gacggaacgc tatttattga ctggtttgga ttgcctgaaa ctatctttga aaaatatccc
     6121 aaaattgtag ccactatctc tggcgcgata ggtgggatag tagttctgat attacaatat
     6181 ctcctccatc tattatctat tatctattat ctattatatc tattatctat gaaaacccta
     6241 cccaacgata aaaagacgcg tttcaagcag gttcgcacca taaccgacat gacgtgcaag
     6301 ttctgctatt ccgagaacgt aacccgctat ggaacgcaaa acgggatata gtagtatttt
     6361 tgccgcgact gcaagtataa gttcggcgat caggatactt tacacggcag acacacgccc
     6421 accacagaaa taggggcatc gttgagtatg ttctttgacg gatggtccct tacgaagact
     6481 agccaactac tcaaagagat atatttcgat aaggtagacc cgtccacgat atatcactgg
     6541 gttatggaat actcactgga agcaataatc attttgaagt ggtacaagct caatctcggc
     6601 aatgtatggg tcgtggacga aacagtcata aatatcaacg gcaaaaatgt ctggttctgg
     6661 gatgtgatag acgaaaagat gtgctttcta ctggcatcgc acatatctgc cacgaggact
     6721 atcgaagatg ttgctattgt aatgaatagg gcatacgaaa gggcgggcaa gtaccccgat
     6781 accattctct ccgacaagct accagcatat ccgaagggca tagcgaggat atttggcaag
     6841 cagaccgtcc atctccaatc gcacgggttc ggctctgcca cgaacaccaa tctcatagag
     6901 cgggtttcat agcacattac acattaaaag aacggacgaa ggtattgcgc gggttcaagt
     6961 tcgtaaagac caccgcattg attttggacg gctttatcat caattacaac tttttccgtc
     7021 cgcatatcac cctgaaagat accacgcccg caaaagaggc gggggtaaaa ccgctcttta
     7081 agacgtggga aggattatta cgagcaatat gacatacact aattttcaag agaacccaaa
     7141 aacgtactaa cgcgctaaga atgtacatca agtgttgact tcccgtaatg ctttgtataa
     7201 actgattaag ttatataact attaccataa agtaataaaa caatgcctcg tatggcggac
     7261 cataagccgg atatcgcaaa acttacttcg cttgcgttca tgttgggacg gctggtgcgg
     7321 gaaggattgc gcgcaaaacg gggaactcct tccggttcgt tgctccggtt tgaggttttg
     7381 tattatgcaa agagccatcc caagccgatg atgctcgata ttgcgcgcta tctttgcgtt
     7441 acgccgcccg cgacgacgct ccttatcgat agtctggtaa aagaaaaatt gatagtaacg
     7501 atccttgacg aacacgaccg ccgcatcgtc cgcattgatc ttacgagaaa gggagaccgg
     7561 tttattgcgc gcggcattca gggaaaggag gacagcttga agaaactttt ttcggttctc
     7621 aacggggaag aacgcgcgcg catggccgct cttttggaaa aaataatcgg cggcaaaacg
     7681 gaaaaccatt aaattaatat gaaggatttt ttttcaataa aaaaattgat tgcggtgatc
     7741 gggattttta ttcttgcggc cgccggcgct gccggagcat ggtggtatat cgcggcgaac
     7801 cggccgccgt cgttcggcac gacaaccgcg aagcgcggga acgtggttgc gtcaatcgat
     7861 gaaccaggta atgtccttac ggacgacgac gtcactcttt cgttccagga gagcggccag
     7921 atcgcgacgg taaacgtgaa cgagggcgac gccgttccgg ccggaaaagt actcgcctcg
     7981 cttgaccagt cgtcgcttcg ggcgggattg gaacaggcgg atgccgggct cgcggcggca
     8041 caggcaaagc ttgacgagct tgtgagcgga acgcggccgg agcaattggc gatcgaccaa
     8101 gccgccgtta cgaatgccgt ggcggcactc ggcgccgatg acggaaccgc gtactcggct
     8161 gcggatgatg ccatccataa ccaaacggat aatttattcg aaaatccgat aaatgagggc
     8221 aatccaatat tttttatttc catgtccgat tcgcaaacgc agattgatat cgaaaatcag
     8281 cgtctcgtca tcggcgcggt gcttgacggc tggtatgcgg cattgagcgc gccgagttcc
     8341 acgccagttt ctctttcgtt ggtcgccaat agcgcattac agcagataca atcatacctt
     8401 gacgcgattg cgcgcgcggt ggacggcgca acgccgagtt ctgcactgac atcaaccgcc
     8461 cttgcagggt acaaagcgga tgttgttgcc gcccggacgg aagtcggttc ggcgatcacg
     8521 gcgttgaccg ccgcggagtc ggctttggaa accgcgcaaa accagcgtgc gctcgccgag
     8581 gccggttcga cgccgcagga tattgaagtg cagaaagcgg tcgtgcagca actgcaggcc
     8641 caggttgcga gcgcccaggt cgcggtggat catgccgatc ttaccgcgcc attcgccggg
     8701 gtcgtgcgga atttgagcgc gagggtcggg caggtggtcg catccggcgc ccccgtactt
     8761 tctcttacga ataacagcgg gttgaaaata gaaacgtatg tttccgaagg tgatgtcgca
     8821 aaagtgaaga ccggcgatgc ggcgaacgta acgcttgatg catacggcac ggggaccgtg
     8881 ttcggggcga ccgtaacaac cgtcgattcc gcggaaacgg tcgtgaacgg cacgccggca
     8941 tataaagtta ctttgtattt cacggctccc gacagccgta taaaagacgg catgacggga
     9001 agcgttcata tcatcaccgc ggaacatgac aatgtcgttg aaattccgtc gcggctcatc
     9061 gtgaacgacg gggacaaaaa ttatgtcctg gtgaaaaatg gcggcatggt cgaaaagcgg
     9121 gaggttgtta ccggcctttc gggcgatgac ggcatgacgg aaataatttc gggcgtggcg
     9181 gcgggcgatg ctttaataaa tttttaatct tatggccgaa cataaacaac aggcggaatc
     9241 aaaaaataat tcccgcggcg gcaaccggac gatcatcgcg atcacggcgg tactcgtcgt
     9301 tcttggtctt gcgggaggcg ggctctactt atatgtatcc gcgcaaagcg tgtatatcga
     9361 ccagtcggtc atcaaggcgc cactcatcaa tctgtcgccg gtcaattcgg gcgttttgca
     9421 ggctgttttc gtgaaggcgg gtgatacggt aacggccgac gaacccgttg cggaagtcgg
     9481 caatgagatc gtgaaagcgc agacggacgg agagatcgtt tccgtcgacc agaacatcgg
     9541 agaattcgag aatgcgctta cgggacaggc ggttgttgcg acgatgattg atacgacaca
     9601 gcttcgggtt gtcggccatc ttgatgaaaa caaagggctg gcggatgtgg cagtcggcga
     9661 tccggcgacg tttaccgtag acgcatttgg cgcaaagcag taccggggca tcgtggatga
     9721 agtgagtccg acgtcgcggg cgagcggcgc catattcgac atttccgacc agcgcccgac
     9781 gaacgaattc gacgtctatg tgcgtttcga tcctgaaaaa tatccggaac tgaagaacgg
     9841 catgtcggcg cgcatctggg tttataaaca ataggattct tttgcgctat gccgaccaac
     9901 gagcaaagta gcacgtggcg ctgggtggtt ttgggaaccg tcattttcgg aacgttcctt
     9961 ggacgcctcg atcagacggt cgtcaatctc gcacttccga aaatcatcgg cgatttcagt
    10021 atttccgtat ccgatgcggc gtggatctcg accgcctata tcctggcgaa tgccgttttc
    10081 gttcccgtat ggggaaagct cggcgatacc gccggccgga aaaaaatata tctcatcggt
    10141 ttcatcggtt tcgtcgcggc atccgccttg tgcgccgttt cgtggaattt gagttcgatg
    10201 atcgttttcc ggattatcca gggtttcgct gtttccgccg attatccgac cgcaatggcg
    10261 atcattgcca taacgttcaa cgacgctaaa gagcgcgcgc aggcgctcgg catatggtcg
    10321 gcggcattcg ccgtggcttc cgttttcggg ccgcttatcg gcgggccgct tatcgataat
    10381 ttcaattggc gcgcggtgtt cttgattaat attccgcttg gccttatcgg tttgtttctc
    10441 gcgatcacct ttatcagcga atcggtggga ataaagcggg cagtcaagtt cgactggcgg
    10501 ggttccgtga cgctcggcgc cgcgctttcc actttggttc tcgtactgga tcaggggcag
    10561 acatggggat ggatgtcggc ggcaagcatc atctgctatc tttccaccgt tgtttttacg
    10621 gctattttta tcatgattga acagcgtgaa ccggaaccga tcgtcaatct caagtttttc
    10681 aagatcggca cgttcgtgag cacgctgacc aataatttca tcgttttcat gggtctcatc
    10741 ggttcgatat tcctcattcc tattttcgcg gaaacgtttc tcggctatga tgcgacgcag
    10801 acggggtatc ttttcattcc gcttgcaatc gccatgatgg tcgccgcgcc gatcggcggc
    10861 cgtctcatcg gaaaagtgaa accggcgtat gtgatattcg cgagcacgtt cgtcgcgggt
    10921 atcgcaatgc tcatgctttc ggctcttgat ccgcgttcta cggcgcttca gatctccgtt
    10981 ccgatgtcca ttatggcgtt cggcctcggt ttcggcatgg cgcagcgcac gaatctcatc
    11041 gccgtcgcgg tgccgcacaa ggatatcggc gaggcctcgg caatccttgc tttggtgcgg
    11101 aatatttcgg gcgcgttcgg tgtggcgatt ttcagcacgt tgcttaacaa taccatcaat
    11161 gccaatgttc ttaaggtggc gcagaacagc gcggtgcgtt cggcgtcgcc gcaggcgata
    11221 cagcagttta tcggacttat cactttaaaa gcgcaggtgt ccggttatgc gtttgttttt
    11281 gaagtcgccg cttttattgt tatgataggc gcgtttgccg ccttttttat taaagttccg
    11341 aaagaagcgg tcacgcgcaa tcacgtcgtc attgcggatt agccgcggag cgatcatcgc
    11401 ggcgcgtttt cgattgttat gcggcgtgct taaaaatacg ttttaaaaaa gtccgacagg
    11461 catcgccgcg gactttttta aatttgagtg cgctcatatt atccgaaagc gaaaacttcc
    11521 gaatattatt tttccataaa cacatcttct tttccgaaac gggatttcgg gaattgcaga
    11581 tacggatcat ccgatgagcg gaagccggcg gcgagaagca cgcacgaagc aagaccgagc
    11641 ggagcaagac cgagtatttc gtcgaatttt ttcgtgtcga atccttccat tggcgtcgcg
    11701 tcgatttcgt tttccgcggc ggcggcgagc gccatgccga gggcgatgta tacctgtcgc
    11761 gtggcccatt cgatgcgttc cggttccgac cggccgacga ctgctttcat aagcatggtt
    11821 ttataatcgt tgagcgatgc cggatccgcg ccgcggtctt ttgccactgc ggcaagatag
    11881 cggtcgatga aagattcatt gatgtccgtt cggacggcaa gcacaaaaag atccgacgct
    11941 tcggtgattt gcggctggtt ccatgcggcg ggccggagtt tttcgcgaag cgcggaatct
    12001 gtgacatgga cgaatttcca tggctggatg ccgaatgaag aaggggagag gcgaagcgcc
    12061 tccatgatga tggcgcgttg ctcgggcgta agcttcttcg agacgtcgta gcgtttcgtc
    12121 gcgtaccgcc aatgaaggtt ttctatagtg gatgccattg ttttttattt tatttaaagt
    12181 ataaggctga tatttttccg gtcaagcagg gaaacgatgc ataggtgcgc attgcgtgcg
    12241 tatatcctat aataatagaa agaaaggtcg aatcaaaaaa acaattcaat tatctatggc
    12301 taaaaaaata ggacgctttg tcatcgccgg ttttgcggtg attgcgtttg tcgccgtatt
    12361 cgttgtagcc cgttccgttt atgccgatga taacggcaat aatggaaatg gcagtgacac
    12421 tccgtcaacg caaatggaaa atcatggcaa cgcggtaagt cagtttgcgc aaacgcaaat
    12481 ggaaaatcat ggcaatatcg taagtcagat tgcaaatgaa aacgaaaatg gcttgacgtc
    12541 gcaggatacg agcgagtcgg tttccctgaa atcaaacggt gatttttcgg tgacgggggt
    12601 aaaggtgaat tccgttgacg catcttcgac atccgtgaac gtgacgtttt acggtttcgt
    12661 aagaacgata agcttggcgg gggccgttat tacgggagca gggcagacga ttgcggtaag
    12721 cgacatccag ccgggagata tcctttcggc gagtggcaat ttcaatgagg caacacatgt
    12781 tcttaatgtt gcgagcgtcg tcgacgtgtc ttctatcagc aaggctaacg cgggcattca
    12841 ggctcagatc aacagccttt tgcagtttgt gcaacagttg caggcgcagc ttagtgcaat
    12901 gctcactaaa tagagaaaat gccgattggt caaaaaaata atcccttcct attgagaggg
    12961 gattattttt ttgcctgaga ttttgaatgc actattgtct ttggaatgct ttccctggtt
    13021 gccacggaac accaagccac ggaagaaacc agcgatcaag tccaagccat ccggcattgc
    13081 gccatgccag gatcaaaaat agctggataa gaagcaggag tggattcacg ctcacggttc
    13141 ccgcaaaaag atagttaaga ttcatgaacg cgccaaaaaa tgccgcgatg ccgataaaag
    13201 cgccgaagat aagtccgagg ccgaccgcga cttctccgta tgtgatgaga tatgaaaaca
    13261 gaacggcgtg gggcattgcc gcatttgtaa ggaagtagcc gtaccatgag gaaacgcttg
    13321 gatgggcatc tcctgtgttc gcgactgcgc ctgcgaagaa tcctcgaatg gcgacgcccg
    13381 cttgcgcccc gatccacgtg gggtttatca ttttttccca ccctgccatg agccatatat
    13441 agccgctata gcagcgaagc ggaagccata gccacgccat ccgtgtgtcg gcgaacaaaa
    13501 accgcgagat attcggttcg gggatttgga tcgagtgttt ttgtattgat tgcattttaa
    13561 caatagaatc ggggtcgtgc gctgctctat ttatcggagc tgtgcgagaa cctgtttgat
    13621 atgataatat aatat
//
LOCUS       DAIDDT010000020        13273 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_20_length_13273_cov_5.951652, whole genome shotgun sequence.
ACCESSION   DAIDDT010000020 DAIDDT010000000
VERSION     DAIDDT010000020.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13273)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13273)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13273
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20_length_13273_cov_5.951652"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3186 a         3651 c         3080 g         3356 t
ORIGIN      
        1 gtcatcgtcc gcgaaggcgg acgacccatg ctttcagtct ttatttctta aagtgtgaat
       61 ccccgccgga gttcctcccg agttttcgag tggcgaggac gacaccatga gaatggcatc
      121 gtttcttagc ttatatattt ttgaacatcc tgtctatttt ccctttcagc gatccgtcga
      181 attgcgcctc gccgtccacc ataatcttca ctcccgcgac aagctccggc atgatgacac
      241 cataaacttc atcttcaggt tttgcaaaat ttttgagcaa tgcccgcggc ggctgtttga
      301 gtccgcgcgc cgacgccact gttatctccc gcacgccgtg ctgtttgcgc atcaggcgtt
      361 cgctttcccg cacgatcttc ggcaggtatc gtccgtctcc gttcctgcgc accaatgcaa
      421 gaaaattttt cgcgacggcg tcgcttttcc ccgcctcggc aaccgccatg gcaagcgctt
      481 ttgcgtaaaa taatacggga tatttcatac tataatggtt cgttcaaaat gaaaatccgc
      541 tccctctgtc attccggcga aggcggaaac ctgtgcttcc aattttaatt tcttaaagcg
      601 cggatccccg cctccgcggg gacgacaaaa aatgttttta ttgcacttct ttcaccgctc
      661 ttgcaatcaa ggcatcgtcg attgccgacg gatcaagctc gaccattttc acgagcgatt
      721 ctttcacgag cgattgcgcg ccgcgcaaaa cctcccgccg cgatgcatct tccttgacgc
      781 gaagcacggc atccatgtct ttcaacgccg ccgcctcctt ttctttcgct ttcgcgagaa
      841 gtttcgcttc gaggactttc gcgtcattct ccgtcttccg caaaataccg agcgcctgcg
      901 cctcggcttc ttttattttt tccttgcttg tttcgttcgc ttccgaaagc cgcacgtcgg
      961 ccattttcgc ttttacaatc ccttcctcga tcttctgccg ccggtcgcgc aacagcttca
     1021 gaagcggctt gtataaaagc agccataaaa cgaaaagcag cacgaaaaaa tccaccgcct
     1081 ggcttataag caggcgccaa tctatcccca attggctgaa catttcttgc atgatgatga
     1141 atttttcctg ttatcgtccg cgaaagcgga cgacccgcgc ttcccgtctt ctcttttaga
     1201 aaacgcgaac tcccgaccgg agtcgcgcat gacgaaaaaa tggttgcgcg gattttttac
     1261 acgaatttga tgatgagcgc tatgaccagc gtgtaaatgg cgatagcttc cgcgaacgcg
     1321 atcgttacga ttgcgaacgt ctggattttt gaacttgcct cgggattccg cccgattgcc
     1381 tccaccgaac ttgcaccgat caaaccgatg gcgataccgg gcccgatggc tccgaaaccg
     1441 accgcgatag ccgccccgag aagccgcata gtgttgagat ccattgttat attttgattt
     1501 ccattgattt tactttcctg cgacctttcc ttcgatcacg catgccggcg cgtttcttct
     1561 ccgtgttcgt cgacaatcgc catactgacg aaaacaagcg tgagcatccc gaagatgaat
     1621 gcctggatga aaccgacgaa tacctccagg aatatgaacg gcatcggcgc gatatacggc
     1681 acgagaaacg ccatgatcgt cagaagcact tcgcccgcga aaatattgcc gaacagcctg
     1741 aacgagagcg agataatttt agcacattcc gagatgagtt caaggatacc gacgaaaaac
     1801 ttgatcggac ttttgaaatt gaaaaatacc gacaggcgcg accgcaggcc gatcgtcagg
     1861 atgcccatga cgttcgcgaa aatgaccgtg atcagcgcaa gcgcaatcgt aaaattgagg
     1921 tcggctgcgg gcgaccggaa aagcggcacg gtttctccgg cgcgcgtaac gatgagcgat
     1981 cctacgccgg gcaaaaggcc gagccagttc gaaaacagca cgaaaaggaa tatcgtaagc
     2041 acgagcggga gatatttttc cgacttgcga cggtcaagca gtaccgaatc cacgatgtct
     2101 aaagcgcctt ccattatcag ttcggcgatg ctttgaagag tattcggtat caacgaaagc
     2161 cgccgccgga tcacgaccgc ggcaacgcaa aaaataataa aaacaagcgc ggaaagcaac
     2221 agcgcgttcg tgaccggaaa tccggctata ctgaaaatct cttcggcgcg gatggaaata
     2281 tggggagaca tgttttatta aaagtccgtc tttgaagatt atcttgaaaa ataaggcgtg
     2341 tcccgtccgt tttaacaaaa aaataagcgc ggacaagccg ttattaagtg gagtataaca
     2401 gaaaaaaacg cctgaatgca agaaaatcct atccggacaa gatacaattt acaaactgct
     2461 ccctatttcc tctacaacct tatcgagcgc catggaatgg gatcctttca cgaggacgag
     2521 gtcgcctttt cggatgagac cctgcactgg ttttttcgcg tcgtcggcgg tgtcgtatga
     2581 aagaatatcc gcgcttttca taccggatgc ctttgccgcc gcggcgataa atttcgcgcg
     2641 cgggcctacc gtcaccagaa catcaagaaa ttccgcggcg gcatcgccga catattcatg
     2701 cgcctcggga gtatattttc ctatctcgag catatcgccg agcaccgcga cttttctttt
     2761 cgccggcaag tcgcgcaagg tatcaagcgc cgcatgcatg gaaagcggac tggcgttata
     2821 acaatcattg agaataaggg aatccttgac ccccggcaaa agctgcatgc gccccggcgc
     2881 cggaacataa ttcccgagcg cttcggaaat atcgacgagg ttcatgccga acgcgatacc
     2941 gaccgcgccc gcggcggcgg cggcatacgc ctgcgctttc ccgaacgcgt atgccacgcg
     3001 caccggcacg aacgcattct gatattcaag cttgaacgaa atgccgagcg gcgcgccgtc
     3061 gcggatgacg ttctcaaacc tcgtcatacg gacatccgct tccctgagga aaccgaagga
     3121 catgacccgc gcgcgggtgc gatttttcaa acgcatcacg ctgtcgtcgt cgccgttcag
     3181 aatcgcgtac ccggccgcgg gaaggcactc gataagccgt cctttttcgc gcgcaacctc
     3241 atccgcgccg gaaaaaaatt cgacatgcga cggaatatcg ccgatggcgg tgatgacgct
     3301 tatttccggc cgcgcgattt ttaacagatt tttaatgtcg ccgggacggt caacggcata
     3361 ttccaaaatc aaaatttccg gatagcgatc cgtttttttt ataagccgcc atgccgaaaa
     3421 cgcgagtact ttcatccaga aaaaaatcct gcggatcttt tcggcgcccg cagccatgtc
     3481 ccgctcgccc gccggaccgc cggtttttcg gacgggctgc ccgtggctca ccagcgaaag
     3541 atcctccgcg ctccaatctc ccagaatggc aagcgaaagc ccgaactcgt tattcaaatt
     3601 tcccgatgat gcgcgcaccc ggtgatgttt cgccagtacg gctgacagcg cacgcttcgt
     3661 tgacgtcttc ccgacggttc cggtaatgcc cacgatcttc ggttcataac gcgctatggt
     3721 gaggcgggca agccactgta atttcacgcg caggattttg gtcaagattt ttttcatttt
     3781 cgataacgga gataaatata tttttttcgg cgccgcacca ctatgatttt cccgcttcca
     3841 tcttaagacg tacgcgcttg cgccgagtac gagacatttc cccctgtcat tgtccggctt
     3901 ggccggatga tgacagaaaa aatttttctt atgatttttt taaaatgcgt atgtcctaat
     3961 ttatttttga aacgcgccgc cgggtcctta ttgtccgcat atgaggtcgc ggcagtttgg
     4021 tattacgtcg cgcgtcgtcc ggtccggagg aatgttgtaa taattcacga tatactgggc
     4081 aagctgttcc catgccggaa ccacggtttc cgccgcaagc gacgtaacgg gtatggtgtt
     4141 caatctgata aatgctacaa aacgcggatt ggacgcaggg ccgaaaccga tatatgaatc
     4201 ggtcaattcg ttcaagtatc cgccgccggc agggttagga atgaacgcgc tgcccgtttt
     4261 ccctgccata gcataaccgt tgatattggc aacctgagcg agatcgacgg catcgaccat
     4321 catctgcgtc acctgctccg ccgtgttcgt gcttatcacc cggcgtatct cctgcggctg
     4381 caatcctgca ttaagatagg ggcgcatcaa tatccccttg tccgcaatgg cggaaaccgc
     4441 gctcacaagc tcaatgggag aaacggcaac tccctgcccg taagcggcgg tgtcccaatc
     4501 gaccgcaggc gctcttggag taagctggct gagattgccg cccacctctc cgggcagatc
     4561 gattccggtt ttctggtcaa ggccgaattt tttaagatag ctcagaaaga tatcgttgcc
     4621 ggtagtgttc tcggcccaga tagcgccggt atttatagag tgctcgatca cctgggtcat
     4681 ggtggtgccc ggaccatatg cgccatgcgt ggtcagattg taattggtga tatgcgccct
     4741 gctgacgtcc acgtatccct tatcgtcata ggttgtgtcc ggcgtgatct tccccgaatc
     4801 tattcccgcc gacatcgtga taactttcat aatcgaaccc ggttcataga ccgtctgcac
     4861 gttggggttc aagaaatccg cgatcgacga cgagctgtaa ttgttcggat cgaaatcggg
     4921 cgtgcttccc atcgcaagga tcttccccgt ctgaggatct tccacgatga cgctgccgcc
     4981 cgacgcattg ttcgccgtaa ccagattgtc gagaattttt tccgcctcga tctggatatt
     5041 cggatcgatg gtcaggttga tatcggttcc ctgcgcgatg gctttcgtat ccgatgccgc
     5101 acccgccagc gtattattat aaaattcctc gatgccgtaa cgcccgcttt cgccgtcatt
     5161 cgtcgcgttc ggtccgacat agcccagaac ctgcgacgca atcggaccca gagggtaaaa
     5221 acgctccgac gccgtatcgg tataaatccc cttgatattc aaattttcga cctgcgacgc
     5281 gacggcaggg tcggcttttc tcacaagcgc ttcgtaggaa tcattcggct tcgacaagat
     5341 tttaaaaagc gaatccgccg gcatgttcaa aatcggcgcc agcgtgtccg ccgcttcccg
     5401 cggatcaagt atcgccttcg gcacggcata aatgatcgga aaattttcat tgatagccgc
     5461 cgggagtatg ccgccgttct cgtccgtcaa ataaatggcg ccgcgatttg cacccgcatc
     5521 cgtcgatgac agcatctctg acgcggcgcg cgcggtgtaa tagccgccct tcaccagctc
     5581 caggttataa aggcggaaaa gcaggacggc ataagcgaga aaaaatactc cgatcaatat
     5641 tgagaagcgc gccatttttt atcgttgaaa aattgcccgg aaattcgttt ttgccgcgga
     5701 ttgattttaa aggttttcgg atagccccgt tgagaaatat tgcggatttt tttcttccac
     5761 caaaccgttc gcttgggcga acgccgcaac cttattaccg tcgagcgccg cgataatggt
     5821 gttgttaagg ccggtatctt gcgcgctcac ggcatcaagt tccgtcttta cggcggcaat
     5881 gttatggctc aggttcaccg tactgttgta aagcgtcacg aggaaaaata tcccgccgag
     5941 cgccgcgata cccaatacgg cgataaggcc attccaaagg ctgttgttct tgttgggttg
     6001 gatataagtc atagatgcgt tgtaattgtt ataaggttgt aatggtaatg ttgcggcgaa
     6061 atagtaaaaa tgatccagtg ttctattgtt gtaatgttga aaattttcat ttaaaacact
     6121 gcatcattac atcatcctga ttgcccttag ttttgccgaa cggctccgcg ggtttttctt
     6181 tatttcctcc ctggaagcgg tgagcggttt tttcgtaagg atcgcggctt tgttttcttt
     6241 cgccaatttc cggaatgccg ccttcacaat gcggtcttcg atcgaatgga aactgatgac
     6301 gaccgcacga ccgccgggct tcaggatctt ttccaaaccg ccgcaaacgc gttcgagatt
     6361 tcccagctcg tcgtttgcgt agatccgcaa cgcctggaac gtccgcgtcg ccggatcgat
     6421 tctcccccgt tcatatcccc gcggcagggc gttccgcacc gtttccgcaa gctctcccac
     6481 aaacatgatc ggttttcgtt tttcgcgttc cgcaatcgcc cgcgcgactc ttctcgaaaa
     6541 acgctctccg ccgaattcaa aaatgatgtc cgcaatctct ttctcgggca tggcacggag
     6601 gatctcccgt accggcttcc gcgaatcatc gtacgtcatg agcagcggct cgcccgccga
     6661 tgactccccg aagctgaatc cccttcccga ctgcgcgagc tgttcggaag aaaatccgag
     6721 atcgagcagc agtccgtccg cttttccgat ttttttctct tcgatgatct ccggtaaatc
     6781 ggcataattg ccgtgcagca atgaaacatt ctcacggctg ccgatacgca ccttgcatgc
     6841 cgcgagcata ttttcgtcaa agtccagccc gatgaatctt ccgcccggca tgatcttcgc
     6901 aaggaccgct tccgcgtgcc cgccgccgtc gaccgtgccg tcaatgatgc aatcgcccgg
     6961 tttcggatca agcaatgcca tcacttcttg taaaagaaca gggatatgag acataaaaaa
     7021 attttaaatc cgcgcttccc gtccatcgcc ctcttgaaga aatcgccctc ctgaaaaaga
     7081 ggatcaatgc tttcagtcta taattcccaa agcgtggacc ctcgcctccg cggggacgac
     7141 agagaaaact aaattcccaa ttcgcctaat ttttccgcaa tcgcctccga ggaattttcc
     7201 gtcttcgtct tgtaggattg ccacgccgca tcgtcccata tttcaatgcg attatataat
     7261 cccgccacga caatctcttt tttaaggccg gcatatttcc tgagataatc gggaatgagt
     7321 atccgtccct ggctgtcgag cgcgacgtcc gttgcgccgg caagcatgag ccgcgcgaac
     7381 gcgcgcgcgt tcgcctgcgc gagcggaagc gcaaccagct ttttcgcgag cgcatcccaa
     7441 tccgcattcg taaaaacaaa aagacagtta tcgattccgc gcgtaataat ggcgccggca
     7501 actaatgcat cgcggaattt cgccggcatc gccatccgac ctttgctatc taaattgtgt
     7561 ctgtattctc caagtaacat gatagtgaaa cgattaagga ttagttatga gcgattggtt
     7621 tttttgtcac cccgaaaaac atgatgcttc aaacgtttat ccgtgatgac aggatgagca
     7681 cccccgtcac ttctgcacag aaagtggcac ttatacccag aattatccac aaccctccac
     7741 ttcgcgccac tttcatttaa taatagtcca ctctgttaca atgtcaaaat atgccgctca
     7801 aaaattcggg cggtgttata agtgttgatt tatctatgtt ttttgaaata acctacttaa
     7861 aaaccttcga gatatgcaca gcatatccac aaaacaaaat aaagaggtct ccacttcgct
     7921 tatttttttc tccataaaat acttttatcg aaacgatcag tatttcaata tttttatttt
     7981 ttacgcgcga aatgcggtaa tatattcagt tttatcgcaa agaaaaaaat aaaaaacagc
     8041 ccgggttcca agctgttttt tttattcttt agatacgggc tctatttttt ccgcgtataa
     8101 aaatgattcc aattgacgcg cggaagcgga tgatattttt taaacttatg ttttgcttat
     8161 ctttcctccg attaaatgaa cggaggaaat gcttgctctg taaatttttt atttataata
     8221 cgccctgtct tcggtcctga aatcaagata ctgcaaattc ttgaatcccg gatcggccat
     8281 caagctctga ataaccggca agtcatcatc ggcgggaaaa cgcaaactga aataaatatc
     8341 cggaccgtca taggtcgtcg cctggatttc ttcgagaccg atatcgttca gccgcacctc
     8401 tttcacgttc aatccgctcg cgcgaagcac ttttatgatt gagataagat tgggaacgaa
     8461 gcgagcgggt aaaatggcgg cgccgagggg cgcgctactc tgcgaataat catgcaccgc
     8521 catgacgacg ctcccttcgg aatcaaaccc gtcctcgaac atgatcccgc tttcgtcaaa
     8581 ccaggagcat tgttcgtttg tttccatggc cattaccatc gttcccgtcg ccgtgccgcc
     8641 ggtattcgca tcagcggccg atcctttcgg cataaaacac cacacggcga acggttttcg
     8701 ttccgcaacc gttatcgtaa tcgtgcgcgc catataatcc ttttttatgt ccacgccgac
     8761 gaggctcgga acggacgcga taacgttcgc cggaatcgca tccggccatg caagcatgtt
     8821 ccccgtccca agcaataatg ctgtaaaatt atttttcgaa aaagcggagg agcggacgag
     8881 cgcaataatg gtatcgctcg caaccgttgt gtttccccgt acgtcgatat tttttatcct
     8941 aaaaaccggc gacttgaaaa cgatccagta agaaccgaaa acgacaaagc acgccgcaat
     9001 aacggacaac gcaatcacga tatatttacg ccgctgtttg cgcttttttt gcgacgccga
     9061 aatataatcg ccgacttcca taattttgtt atgttttaat ggtgcggcat tgccatgatg
     9121 agaacccgtc tcaaaagcag cttttcaggc aaagtccgcg ggttgtatgg aaattttatg
     9181 aaaccctgtt tcatccgccg gttcgctctg agaaaatgga tgcgtccgcg cattcatttt
     9241 ctcatcgctg ccgacggctg cgaaccgcga ttccctcctc ggaatacccc gcggcattcc
     9301 tcgtgcggtg tccgcgttgc tcgctcggaa atttccataa tcccgctggc ctgcttttgg
     9361 aacaggattc tacgcaccgc cgcgccgcga cgcggtacgg gaacgcctaa acactaccat
     9421 acgccttccg ttgctatccg atcacctttt tcccgacata tttttgcaac acttccggca
     9481 cttctattgt gccgtctttc atctggtagt tttcaaggat agcgatgaaa atgcgcgggc
     9541 tgggcagcgc ggtattattc aataaatagg catatttttt ctcaccgttt ttatcgacat
     9601 acttcacgtt cagcctccgc gcctgccagt ccaaaaaatc gctcgcggaa cccgtctccg
     9661 cccaccggtt cattccggga agccacgcct caagatcgaa cgcgcgatat ttccccgcgc
     9721 tcatgtcgcc cgaacaaatc tgcaggcgcc ggtacggcaa tccgagctcc tcgtgcattg
     9781 cgcccgaaat gcccgtcatc tcgtcctgca gcgcgttcgc tttttcgatg tcggcggggg
     9841 caaccaccac ctgttccact ttcatgaatt catgcacgcg gtacatacct ttcagctctt
     9901 tgccgtaact cccgatctca ctccggtagc actggctgta accgcatgtt tttatcggca
     9961 ggtcttcctc tttcaaaacc tcgtcggaat aatatgccaa aagcgacggc tcggcggtgc
    10021 ccacgagaaa ttttttatcc ttactcgctt cgccggtcga ttctttatcg ggagtggcga
    10081 tctggtagat ttcatcaacc ccgggatcgt attcaagccc tttgaaatat cccgacccga
    10141 aaagagcgga acttcggaca agcgtcggcg gaatcatcgg cgcatatccc ttctcgacca
    10201 tcatatgcac cgcatacatc ataataccca tcgcgagcag cacaccgtcg ttttttatat
    10261 agtacccgcg gaatccggcg accttcgcgc ctttttcaaa atccaggata tcgagcgctt
    10321 gtccaagcgc gacatgatcc ttcggttcga aatcgaattt tttcggcgcg ccccaatggt
    10381 aaacttcgac gttgtccttc tcgcttttcc cgatcggtgt atccggcgaa ataatattcg
    10441 gtataagcgc catcaactcg tcgaatttcc tctcgatgcc ggcaaaacgc gcttcttcat
    10501 aaagctgctt gaacttttct tttaactgca tcccctcttc gcggtccttg gtctttttct
    10561 gctcggcgcg gatcgcttcc gcggactgga tgagctcccg cctcgtttta tcggcctcaa
    10621 gcagcgcgtc gacaagagcc gggtcgatac cctttttcac cgcaacgtct tttataatat
    10681 cgggattttc cctgatgaac ttgatatcca gcattgctct ataatatcac acgaagaaaa
    10741 aatacgaaaa ttaatcctgc gctttcttaa taaaaacgga taaaaaaaac ggctccgtga
    10801 aatagggagc cgtttgaaca aaaagagcta cagaaccgtc gcggcaatct ctgcaagttt
    10861 actgcgcacg ctccgaggga acgtaacgtg gccgaaaagc ggctggcctt tcattttctc
    10921 gatgacgtag gtcaatccgt tcgacatcgc gtcgagataa gaattctcga cctggtcgat
    10981 gtcgccggtt aagacgatct tcgtgccttc cccgacgcgg gtgatcattt ttttcaagtc
    11041 cgcggggcgg aaattttggg tttcgtctac gatgacatac gttttataaa gcgtcctccc
    11101 ttggatgaaa ttgatcggtt caatttcgat attccttccc aaaagagggt cttttggatc
    11161 ggacgatctc ttcttctttg aaacttccgg ttcgctgttc cttgggaaaa ggcgcaggag
    11221 atcatgaatc ggacgtttcc atggagcaaa tttctcgtca agagaaccgg gtaaaaaacc
    11281 cagtggctgc ccgatctcga cgttggaacg atagacgata atccgttcat aaagtttttt
    11341 atctacctga cggattcccg caagaagagc catgagcgtc ttcccgcttc ccgcaacgcc
    11401 cgcgagcgtc acaatctcca cggccggatc cataagaagg gcaagagcga gcgcctgctc
    11461 tctgttgcgg ggctcaatat cctggcgctt ctcttctttt tctttcggct ttggtcttgg
    11521 ctttggaacg tgaataaaat gaggggtgcc ctcgctttct ttgtaaatgg aaaggcgttc
    11581 ctcgtcgccc gcagaaaaaa cgcaacattg attcggaata acgcggggaa tcgaaaccat
    11641 ttttttaaga tcttcgcgaa ggccgcctcc attacaaatc aaccggctca cgtcaccgaa
    11701 atctccctta tcgatcggga tatggataat gccggagtaa agatggctat ccggctcaac
    11761 gaccctgccc tttaggtaat cttccgaaga gacgtcatat ccgtgagctt tgacccgaag
    11821 cttaacatcc tttgtgacga tagatacggc cgtgatgtga aaattttttg agaacaccgt
    11881 gacgatatca tgctcgctgc cgcgggcttt caccttccgc gtccattcct ccctcagcgt
    11941 ccatttcaac gcaatgctga taatggaact gtaaggcgca tcatccggca aagaaaaacg
    12001 gcgcggaaca tatccatgat gatccacaag aataagtcct cccgaagccg ttttcacgcc
    12061 atgataaaga tcgctttctt tctcgcataa tgaatcgaga atcctgatgg cttgctgggc
    12121 atatgccgcc gctccgtttc cgccgtttat aagttcatca aggacagaag ccggaacaac
    12181 aacaatattg tcctgaaaac tcaggatgga tttcggatcg tgaagcaaaa gagatcggtc
    12241 aacaataaac acctttacgt tttttggctc aacttgtgcg tcctttccta ccataaaaac
    12301 ctccttttat attaaattgt gaagagcaaa taaccgaatt tattatacat ttttacaaaa
    12361 aataagcaaa tcaaaatcgc agcgccataa tggaaataaa atacgcggcg taatcgtgat
    12421 tccattgtcc ggcatcgcgg cgcataccgc ggaagggaag aataaaaaat gcgctcccaa
    12481 taaaatcgag agcgcgtaat atttatccgg cgatagcgac acgacctccg ccgccacaaa
    12541 cacggcaggt cacggttccg cttccgccac actgcgcgca attataacta tcaataccgt
    12601 attggcgctt gccggtaccg ccacaattga ggcattttat ctggcccttg ccggaacact
    12661 tattacattc cttccactcc ttccacttcc acctcgaatt atccttcaaa gttcccatta
    12721 aagttcccat aaaaccacct cctatttttt taagttatga agactgcact ggctttagta
    12781 tcagcaatta taatttgctt gtcaagcaat cttcgtccat aattgtttca tggacgacat
    12841 tgcggaaaac cggcgggcgc ggcacgatta tgaaatcgaa atggccgccg aagcaggcat
    12901 tgaactcacc ggacaggaag tgaaaagcgc gaaaggcggc cggtttcaga tcgcgggcgc
    12961 gcgggcgctt atccgcggcg gcgaagtatg gctcgtgaat gcgcatatcc ctccctatca
    13021 gccgaaaaac gcaccggcaa attacgaaga agaccgggcg cgccggttgc ttttgcggaa
    13081 agacgaaatt aaaaaattca cgggggcgct taacgaaaaa gggcgcatgc ttattcccct
    13141 gcgcgtctac ctcaagcgtg gtttcataaa aatagaactg ggtctcggcc gcatgaagcg
    13201 aacttccgat cggcgcgagg atctgaaaaa gcgcgcgcac atccgcgaga tgcggcaaaa
    13261 taaaatatag aat
//
LOCUS       DAIDDT010000021        13014 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_21_length_13014_cov_5.385561, whole genome shotgun sequence.
ACCESSION   DAIDDT010000021 DAIDDT010000000
VERSION     DAIDDT010000021.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13014)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13014)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13014
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21_length_13014_cov_5.385561"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3522 a         3535 c         2960 g         2997 t
ORIGIN      
        1 ctatgaaacc gaaaattccc gccgcatgcc gctcggacca aaaatcatat tcagtatcgc
       61 agatatcggt ctgtaacgcc ctgaggtctt cgcgcgcatc tattatttta caaccctcaa
      121 ttggcgcggt atttttagtg aattcttttt ttgcttcgcg agcacagcag aattcgcgtg
      181 catattcccg gagtttttca tctatcgcct tctgtagaat ctttatctct gccgaggaag
      241 ttggatgtgc aaatccaaaa cgatcaaaaa acaatttttg ctgttttgtc attgcttctc
      301 cgataagaaa atatactgct ccaagcatca tgccaaccag gactgaaacc ccaaagaaaa
      361 agatgacaaa tcccttcagc gtctgaatgt agtgcgtctc cctgccgatc atgagaaaaa
      421 aaacgaccat gccaaccatg agcactacgc tcactatcca aaactccctg ctttttctca
      481 tttcattgag atttttcagg ataaacagat catcttccat aaatcaatcc tccttattct
      541 ccttttcgga gaaattttaa tttttattaa gtttttaatg tgcataactt tcgacgcgcc
      601 gagcttaaca gccaagaatg ggatttatac cacccgaaag ctatgcctat tataggctat
      661 atataataat attgtcaagt ggcgcggagg gcggatcggc ggcacgaaaa taaatttttt
      721 gcattgatta attccgattt ttccgctatt ctttaggctt aacgaaggat taaagccgat
      781 gccaaacgat aatgtcattc tgaaattcaa taaggtttct ttcgagttcg gacacgcaaa
      841 acccatcctc aaagaggccg atttttcgat ccggcgcgga ttgaagttcg cgctcatggg
      901 acagaacggc gccggaaaaa gcacgctgtt cagcatgatc acgggcggcc tcaagcccga
      961 aagcggatcc atcgccatcg aaaacaaatt gagcatcgcg atcgcgaagc aggtaatccc
     1021 ccggaatcag atggatctta cggtaaaaga atttttcgaa tcgctgttcc cgaaaaaaat
     1081 atacgacatt gatccgcgca tcgcggaggt cttcgagatc gtccaccttc ctgttccgtc
     1141 cgaccggaaa gtgcgctcgc tctccggcgg ccagcaggcg cgtcttctgc tcgcgtccgc
     1201 actcatccaa aaaccggatc tgctgctgct cgacgaaccg acaaacaatc tcgacgccga
     1261 cgggcttgca caccttacgc agtttctcgt cgattacaaa aaaacctgca tcgtcatttc
     1321 gcacgacgcc gacttcctga atgccttcac ggacggcgtc ctctatctcg acgtcttcac
     1381 gcacaccgtc gaaaaatacg tcggcaacta tttcaacgtg gtcgcggaaa tttcggcgcg
     1441 gattgagcgt gaacggcgca aaaacgtccg gcttgaaacg gacatcatga accgcaaaga
     1501 acaagcgagt ttttttgcac aaaaaggcgg ccatatgcgc gacgtcgcgc gcaaaatgaa
     1561 ggaagcgatc gaaaagctcg aagaggaaaa agtggaaacg cgggaagagg acaagaccat
     1621 ccgtaaattc gcgatcccct gcgaagaaga cctcgcggga ccgattatcg ccatccatgc
     1681 ggtgtcgctc atggcgcacg ggaagccggt gaagaaaaaa gtgaatgtcg tattaaaaca
     1741 aaaaaatcat cttctgcttt ccggtccgaa cggcatcggg aaaacaacgc tgctgcagga
     1801 tatcgcttca gaaaaatcgg cgggtgcaat aataacgaaa ggcatccgcg tgggctatta
     1861 ccggcaggat ttctcgacgc tcgatcccga aaagacggtc tatgcatcgc tcgcggaagt
     1921 gatgacggcc ggcaccgaag aaacgctccg ttcggccgcc gcgggatttc ttttgaacgc
     1981 cggcgatctc aaacaccaga tcaaaaacat ttccgaaggg cagaaaggac tcgtcatgtt
     2041 cacgcgcctc gtactccagc ggccgggact gcttatcctc gacgaaccga cgaaccacat
     2101 caatttccgc catctgcccg taatcgccga cgcgctcgac aaatatgccg gcgcaatgat
     2161 cctcgtaagc cacgtcccgg acttcgtaaa aaagatccgc atcgacgaaa cgctcgacct
     2221 cgcggcgctg ataaagaaat aatgcgctcc ccgctgtcat tccggaaaat gcgacgcttc
     2281 gagcgtttat ccagaatcca gattcgaatt ttaattaaag agtattaacc tggatcctgg
     2341 atagccaaaa cggcttccag gatgacataa aaacatcatt atgcttatcg acaacatcat
     2401 cattaaagcg tcggcgggca agggcggcaa gggcgccgtt gccttcagta aaataaaaat
     2461 gagcctcggt cccaccggcg gcagcggcgg aaaaggcggc agtgtttatt gcgaggcggt
     2521 tgccgacctc ggcgcgctcc gacaattccg ttcaaaaaaa gttttcaccg ccgaggacgg
     2581 gcgggacggg cgcccggcat tccgcgacgg ccatgacggc cgcgacctca tattgttcgt
     2641 cccgcgcggc acagttatcc ataacctgac cgcaaaaacc gaccatgaac tttcgaaaat
     2701 cggcgagcgc gtgcaagtcg tgaaaggcgg gaagggcggg aagggaaatt ttctctaccg
     2761 gtcggcgacc aacacgtccc ccacgcaatt ccagtccggc ctgcccggcg aggaatgttc
     2821 cctacacctc gaactcaagc tcatcgccga catcggcctt gtcggcctgc cgaacgtcgg
     2881 caagtcgagt tttttgaacg ccgtaacgaa cgcgcaaagc cccgtggcgg attatcattt
     2941 cacgacgctc gaaccgcatc tcggatcgta ttacggcctc atcatcgccg acatcccggg
     3001 cctcatcgaa ggcgcggcgt ccggcaaagg tctcggcata aagttcctcc ggcacatcga
     3061 acggacaagt atcctttttc atttcatcga tgcgaaaacc gacgatcccc gccgcgacta
     3121 tgacatcatc cgcaaagaac tcgacaccta taatcccctg ctttcaaaaa aacaggaata
     3181 tgtttttatt acgaaaaccg acgccgtgga agaaaaactg tgcgcgaaaa caatgaaaaa
     3241 actcgccgcg catatccgtc ataaaaaaat atttgaactt tccgtcaacg atccgcaaaa
     3301 aatggcggcg gcgaaaaaac ttttgaacga gataaaggac gagatgattg aaaaataaaa
     3361 tcctatatcc gctccgtcgt tcccgcattc gcggggatga cggaaaagaa ataaaccaac
     3421 catggaaaaa acgtccgaaa cagcggcaaa gttcgccgca tcatacgaac ggctcaatcc
     3481 cgagcagaaa aaagcggttg atacgatcgc gggcaccgtc atggtcgtcg ccggccccgg
     3541 cacgggaaag accgaagtgc tcacgatgcg catcgcgagc attatggaaa aaaccggcac
     3601 gccgccggaa aaaatcctcg cgctcacgtt cacggaatcc ggtgtttcct ctatgcgcaa
     3661 gcggcttgcg gaacttacgg gcggccttgc gtatcgcgca acgatcacga ccttccacgg
     3721 cttcgcgaat aacgtcataa aaacctaccc gagccgcttt cccgagatca tcggatcgag
     3781 caatatcaac gaggttgacc agatccgcgc cgtaaaaaat atcatcgacg cgcttcccct
     3841 gaaagagctc cgtccgtacg gcgacaaata ttattatctg aagccggcgc tcggcgccgt
     3901 aaacgagctc aaaaaacagg acgtgacgcc ggcgcaattc gcggcattcg cgcgcgaaga
     3961 aaaggaagca accctgaacg cggatgatct ttatcacgag aaaggcgcgc ataaagggaa
     4021 aatgaaaggc gtttaccggg aagcgctctc acacgcggca aagaacgagg agctcgcgaa
     4081 aatatacgag gcgtacgaaa aatcgctccg tgcgacgaaa caatacgatt acaacgacat
     4141 gatcatgtac gtcgcccgcg cgctcgagca tgacgacaac ctcaggatta cgctccagga
     4201 agaatacgat tacgtgctcg tcgacgaaca gcaggatacg aaccgcgcgc agaaccgcat
     4261 tgttgaattc cttgcaggag ataatgcggc gcatccgaac ctgtttcttg tcggcgacga
     4321 aaaacaggca atctaccgtt tccagggcgc ctcgctcgcg aatttcgaat atttcaaaaa
     4381 acgctatgaa aacgtatcgc ttatttcatt aaaaaataat taccgttcca cgcaggcgat
     4441 cctcgatgcc gcgcaggaac ttttccccgg ctatcccgag cttgccgctc aaaaaggata
     4501 ctccgaaacg ccggttgcaa cggcagcgct ctcatctccc gaagcggaat attatttcat
     4561 cgcggaaaaa ataaaggagt tgctcgccca cggcgtgaaa ccggaagaaa tcgccgttct
     4621 ttaccgcgaa aaccgggacg ccgcacccat cgcccgcgcg cttgaaaaac agaacatccc
     4681 ctctaatatc gaatccgacc aggatgtcct gggcgacccc gacatcaaaa aactcttgcg
     4741 catcctccgt gcggtgcagc atttcggttc gccggttcag tttttcgaac tgcttcatgt
     4801 cgactttctc gacatcccgc cgcttgatat ctataaactg gcatccttcg cggcgaaaaa
     4861 aagaatgaac gtgtacgaca tcgcgcgctc cgaaaccttg ttcgatgaag ccggcgcaga
     4921 atcaaaggaa aaaatactcg agctctatcg caaactttcc gattggaaac gctctgcgga
     4981 aaatcacggc gcgggagacg ccttcgaaac gatcgtgcgc gactcgggat ttcttgccta
     5041 tctcttgggc cagccggccg ccacagaaaa gatcgcaaaa cttcacggtc tttttgaaca
     5101 tctgaaatcg ctgatcgaga acaggaagaa ttacactttg catgatttct tcgaccacct
     5161 cgatctcgtg gaagagcacg atatcgccat aaaaagcccc gaaacacgaa acgcgcccgg
     5221 gaaggtacgc cttatgaccg cacataaatc aaaagggttg gagttcgaat atgttttcat
     5281 cgtgaacgca atcgacggaa aatggggttc gcggcgccgg ttggaacaca tcaaacttcc
     5341 ccgcaaaatt tatgcgattc tggaggatgc ggaaaccaca ctcgaaaaag acgatgacga
     5401 gcgcaatctt ttctacgtgg ccataacgag agccaagaaa gaaatattca tcacatggag
     5461 cgggaccggc cgcaacggcc gcgaacaact gccatcacaa tttataaatg acataaaacc
     5521 cgagctcatc gcgccgtgcg acgccgaacc ttatgaaaaa gaatatgccg cgcaccgcga
     5581 ggtcgaattc gcgcccgcgc ccgcgcgcga accggagctc gccaacaagg aattcctgaa
     5641 ccggctcttt gaggaacgcg gcctttccgt cacggcgctc aataattacc tcgaatgccc
     5701 gtggcgctat ttctacgtca acctcatccg catcccggaa gcgccgaaca agcatctctc
     5761 ttacggcaac gccatccatg ccgcgctgaa aaatttcttc gaccggcaga acggcgatgg
     5821 caaaaagatc gcagcgaaag aatatctcgt ccagcgattc gaggaagcgc tagcgcgcga
     5881 acccatcgag gaaaacgatt acgaggaagc gctcgagaaa ggcagaggcg cgctcgcgag
     5941 ctattatgac gcctatcgcg atgcatggac gcaaaagacc atgaacgaat tcaagatcga
     6001 taatattgcc cttgctcctg ggataaaaat cttaggaaaa atagataagt tggaaatcgg
     6061 cgatggcggt aaaaacgttg tcgtctcgga ttataaaacc ggaaaaccga aatcgcggaa
     6121 ctacatcgaa ggaaaaacaa gagaaagcgg cagtggcgat tataaacgcc agctcgtgtt
     6181 ctatcggctc cttctcgata aaggaagcga ttataaaatg acgtccggcg aaatcgactt
     6241 catcgaaccg gacgaaaaag gccgccagcg caaagaatcg ttcgtcatca caaaagaaga
     6301 agtcgacgaa ctcgtgaaaa ccgttctcga tgccgcgaaa gagatcaaag acctcgcctt
     6361 ctgggaccgc acctgcgacg atccggattg taagtattgc aaactgaaga aagcgattat
     6421 taaaaatagt tgagcgcgat cagcaagccc ctccctctgt tgtcgttatc cgcgaaagcg
     6481 gataacctac gcttccaatc ttttaagagg tgatggaata aaaaatcgcc tcaaaattgc
     6541 cgacctctgt ttcactaaaa aagctgcgga cccgccggaa cttgtcccga aaatctccga
     6601 aggcgcatcg cctgagggta gatttacgtg gatcctgtct ttgcctgcga taaggttatg
     6661 caatgtcctc attcggagat aaacggaatc cacgcaactg caccactcgc ctttggctcg
     6721 caggcatttg cgggacatcc gattcctcgt ctggcaagaa caagatctta ctatataaac
     6781 gacgagctca tcatccacgg gcaagatcaa aataacaaaa atacgattaa aactatctaa
     6841 aattctccaa cgaaaacata aaacgaccac caaagtggtc gctttatgta ccatacagcg
     6901 aagcccgcca ttacgcagac tgccgtcctt atgaaccata atactattgc cccaatcaaa
     6961 gtcaattacc gtttaatgat tttttaatct gcataaaaac gcattaaaca atgatcccgc
     7021 cgcctaacat gtgcccgttt tttgaataaa aaaccgccga ttgtcccgct gcgacgaatt
     7081 tctgcggcgt gtcaaaaacc agcattatct tccgcttttt atccttatta tccttgccgc
     7141 gcgatgcgga atacattatg cgtgctctta caagcggctg gcggtaacgg acacgggcaa
     7201 gcaccggcac gccttcattt gttaattcaa ttgaatgagt aaataaagga tcaataaaat
     7261 gaacatccgt aagcttgatc tcttttttat agagcgccgg atgatcgctt ccttcggcga
     7321 ccactactgt attttttttc acgttctttt ccgcaacgta gaacggcttc tgctctccgc
     7381 ttcctttcga ctttaaactt ttaacgttat aacgtgtccc cagatgtcgc tgccctatcg
     7441 tatagaacgc cgcgccggca tgctccccta tcgtttcgcc tgttgtcgtc ataagcacgc
     7501 ctttcttttc cggaatataa ttcttcaaaa aatccgcaag cgataccttc cccaaaaaac
     7561 agatcccctg cgaatccttc ttttccgccg tggacagccc ggcttttcgc gccatcgccc
     7621 gcacctccgg ttttttatat tttccgatcg gaaaaaggca atgcttcaac tgctcctgcg
     7681 tcagcgtcca aagaaaatag gattggtcct tgtttttatc tctagcagct tgcagaacgt
     7741 aaaatgtaga atgcagggcg gaatctttct ttttcgcccc tttcccaact ttctgcgttc
     7801 cacttctttc ttcctttctc ctttctactt tctgcgttct gcgttccaca tagtgaccag
     7861 tggaaacgta atccgctccg gccgcacgcg ccttctcgaa aaacaatccg aactttatct
     7921 ccttattgca catcacgtcg ggattcggcg tgataccgcg ccggtagccg tccaccatgt
     7981 attccaccac gcgcttttta tactcctcct cgaaatccca aacataaaac gggatgccga
     8041 tatgttcggc aacgcgccgc gcgtcctcgg catcctgctc ggagcaaccg tccagattat
     8101 aacaacgcat aaaaacaccg acggcatcat agccatcgtt cttcaaaagc agcgccgcaa
     8161 cggatgaatc cacgccgccg gacatcgcca caaatacggt tttctttttg ggaaattttt
     8221 tcatatgcag cctaccaatc tgtcatcctt aaattttgag tccgcgaaaa atatccagga
     8281 tccaggatta attctctttt aattaaaaac tggattctgg ataaacgccc gaaaactcgc
     8341 atttttcaga atgacaagag ggtttacgcc cgttccgtgt attcgcccgt ttcggtattg
     8401 acgcggatca tgtcgccttc gttgataaaa agcgggacgt tcacttttgc gccgccttca
     8461 agcgtcacct gtttcgtgcc gccctgggcg gtattgccgc ggatcgcggg cggcgcttcc
     8521 gtgactttga actccatttt gatcggaatt tccactttga tcactttgtc gttaaacaac
     8581 gtcgtcgtca ctttcgtgtc cggcttcaaa aattttccgc gctcgcccat gagttcggcg
     8641 tcaagcgaaa aacggttctt cgtatttcct tcctcgttga accagtactg tccgcggctt
     8701 tcgtaaataa aaatcgccgg cttccgctct atttccgcct cttcaaacgt gtcgctcgcc
     8761 tgatagttcc gatccagaat tttccccgtc atcagattgc gtatcttcgt ctgcaccacc
     8821 gccttccgct gctgcatgcg caaaaaatgc gtctccagaa cttcgtacgg agcgccatcc
     8881 agaataaaca gggttccttt cgtgaggtcg gtataagaaa gcataggtat agatttattc
     8941 gtttattatg aaaagattat aggcgcaaaa atgccccgac gcaagaggcg gggcattttt
     9001 catagaacaa ttatcgaggt tccgcctcga taattttacg ctccggattc cggtgcggtc
     9061 tccctcggag cacgcgtgct tccttccggc tcttcaatct tcaggtttac atgggcattg
     9121 ttttttacgc ccacgatcct cagaagcaaa cggagcgcct gcgcggtcac tccttcgcgg
     9181 ccgaccacct gacccatgtc caactggttc acgtgaagcg agagcaaaac gccccgctca
     9241 tcaaccttcc gttcgacctt cacatcatca ggatggtcca cgagcgactt caccaaatat
     9301 tcgagaaatt cctgttccgg cgcggcagat atattcgcca tagattcact ggttattctt
     9361 tctttctttt gcccgatgat aaattttacg gttccaccat cggaccatcg accttttcgt
     9421 tttcaatcgg aattttttat ttcaaaaagt gcaaggtaaa atcgcggcgg cggtttctta
     9481 taccgtagcc gcaaccgcaa ccgacgcctc agttttccct ccggctttca cggaagccgc
     9541 cttcattttc acggcttttt tggagaccaa aatgatcccg ttttttacga gaagattatg
     9601 cgccgtcacg gtcggctggg cgcctaccga gagccaatgc aaaacgcgct cttttttgaa
     9661 cgccgccgct tttgtccgcg gattatacga tcccaaatct tccacgggcg gcgcgcccat
     9721 ttttgatttc tttacggccg taaccagccg atagctcggc tgatgttttt tcccgatccg
     9781 ctgtaatttt attgccaaca taataataga aagtataggg cacgaccatg ctccgcgtca
     9841 agtttaacgc gaaccgtttc aaaacagaaa gaatggtgat attatggata tattatggaa
     9901 attttctttt ttatcgtcat cctcattgcc ctcgccgccg gattctggtt tatttcccgg
     9961 acacttaaaa agcaatccga aaagaaaccc gataataatg gccaggcgct cgtcatgctg
    10021 caaaaccaga tcgcgagcca gcagaatcag atcaataacc aaatgaacga actgaaccgg
    10081 acattggacg caaggctcgg cgaatcgaca aaagcaatcc atatgcaatt cggcgaaagc
    10141 acgaaaataa tccgcgacgt taccgagcgg cttacaaagc tcgacgaaac aaaccggcaa
    10201 gtcgtcaatt ttgcagacca gcttaaaagc cttcaagata tcctcaaaaa tccaaaacag
    10261 cgcggcgtgc tcggcgaata ttatcttgaa gctcttttaa aaaatgtctt gccgcccgga
    10321 agctacgaga tgcaatatca cctaggcaaa gacgaaaaga caggcaatga cctcattgtc
    10381 gacgctgtcg tatttatgaa ggaccgcgtc atccccgtcg actcgaagtt cagtttggaa
    10441 aattataacc ggctcgcgga agcgaccgac cccgtggaac gcaaacggct cgaacgggca
    10501 ttccgcgacg atctcaaaaa tcgtattgac gaaacgtcga aatacgtcct gccggaaagg
    10561 aatactatgg aattcgcatt catgttcatt ccgcacgagg cgatctatta cgatcttctt
    10621 gtctcccagg tcggctcgac cacggtaaat gcgcgcgacc ttatcgaata cgcttttaaa
    10681 gagagacatg tcatcatcgt atctcccacg tcgttcctgg catatttaca aaccgtgctt
    10741 cagggattaa aagcgctcca gatcgaagag tccgcaaaag aaatccgtca taatgtcgaa
    10801 aaattactcc agcatcttaa gtcctatgaa agtaattttg tgaaaatcgg aaaccatctc
    10861 gggacaaccg tcgccgccta caccaacgca agcaaggagt tcaaaaaagt cgataaggat
    10921 atctaccgca tcaccggcga agccatcggt ctcgatattc ccgtaatcga aaaaccggaa
    10981 gagtatgagg acgaattata aatggcaccg cctctcagct atcctgtttt ggacggtcgt
    11041 ccgaaataag acagcagaga ccgcacaata gaaggaaggt cgcaaagaca aaaaaatccc
    11101 cgctctcacg gggacttttt gtatactcta tatcttcgca atctccgttt cggagtaaag
    11161 ctggcggtgg ccttttttct tccggaaccg cgttttggaa tgatatttga aaacgatctt
    11221 cgtccggccg tgaccgttct ttacgagttt cgcctccacg gtagcgccca caatatacgg
    11281 cacgccgatc gtgaccacgc cgccctcgca cttcaaaagc accttgtcga aaataacggc
    11341 cccgccttcg gcaatgccga gcttttccac tttcagcttt tctcccgcag aaactttata
    11401 ttgcttcccg ccggtttgta taatcgcgaa atcttttgtc atggaattag tttaaaagaa
    11461 aataagaaaa gattggaagc gtggatcctc tcccgagttt atcccgtacc acgatacgag
    11521 acggggatga cagaaaagaa atgcgccgat tgcgaaagtt ctgttatcaa ttgatgctca
    11581 gtatagccgc aaaggcgata aaaagcaaga cggcaaaaag cacggatgcc gcggcggtaa
    11641 tcttccccaa aaaccggtca tgttcctcca gttccgtcgc aataaaaaaa ttcatgacgg
    11701 taatgatgac gcccagaatt cctattgtcg ctatatccca tagattgcct attttcgtga
    11761 tcccgttcat atcgtcgaaa tgaatgacga gcggcgaacc accggcggcg gcctgcaatc
    11821 caaaatacgc ccagcaaaaa cctccgagcg cgagcgcaat agccgcgagc gacgcggcgc
    11881 tgaccgcctt atgtttcata attttttcca tcatcgccat aattattatt attaattact
    11941 tccgtttcaa taaccgcgga aatgaaagta gtttcccccg tattctaaat tctctattct
    12001 atattctaaa tgctctattc tatttctccc ctcttaaaat cgccgctgcc gtatccggat
    12061 cgacgatatt gatatcgatc tccgtcgaat attcaaaccc gccgaaaacg gatgttcgcg
    12121 gcaccacttc gatatgccag tgatgataaa aatatttgcc gtgatcaagc ggagcgccgt
    12181 ggatgaaaaa attaaagtcg ggatcgttcg catatttttt tactcgccgc aagaccgatt
    12241 gcaaaagcag ggccgccgcg cgaatgacat ccggcggcgt ttcgctgaac gcggaaatat
    12301 gcgctttcgg aaaaatactc acttcaaacg ggctttttga gacatacggc gcaaaggcga
    12361 cggcgaattt gttttccgcg acgatccttt ttttatacgc gcgctcttcg cggatgatgt
    12421 cgcaccgcac gcaacggcga tgcttcctga aatacgcctt cgatccggca agcgaacgcg
    12481 cgatatgcgg cgggatgatg ggaagcgtaa ggatctggta atggggatgt ccgattgaag
    12541 cgccgaccga cggcccccaa ttacagaatg tggcaacata cgaactgcgc ggatctttcg
    12601 ccatcatgcg atgccgttcc cggaacactt caaaaacctt tgccgccatg gcgggactga
    12661 ggtctccgaa atttttttta tggtcgcgcg taacgacaag gtcatgatgg ccgattcccg
    12721 cctttgcgtg ataaattccg tgcgataacg gcacggcgca ggaaagcgct tcgtggctca
    12781 gcgccggata tttatttgga agaaggacaa tccgccagtt tttctcgttc ggatacgcgg
    12841 cgatcggcga cccattgccg gatttttgaa gatgttcaaa ggggcatgcg cggagaggcg
    12901 tcggcatgcg cggcgtattt tttttatcaa gaaaaacagg ccgtttcgcg cgtcccggcg
    12961 cgataatggc ccaatccccc gatgcgatat cctggcgaag ttccgacatg aaat
//
LOCUS       DAIDDT010000022        12986 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_22_length_12986_cov_5.124487, whole genome shotgun sequence.
ACCESSION   DAIDDT010000022 DAIDDT010000000
VERSION     DAIDDT010000022.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12986)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12986)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12986
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22_length_12986_cov_5.124487"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3349 a         3527 c         3110 g         3000 t
ORIGIN      
        1 ccaccagata gatagagcag aaagttaagg ttaagaaaaa attaaacaac aattaaataa
       61 tggttaaaaa atctgaagag aaagaccgtc cacgaataaa gccgccggag atccggaggc
      121 ggcccgccga aaacgacagc acccgtctcg gaaacaggcc gacggaccct cgttcccaaa
      181 aattttcaac gggacgcgat aataaacatt caccatacgc tccaaaaaaa ttcggagggc
      241 agtccggcta tagccaaagg aataccggcg ggcagtcccg ctccgcgtct tccgaaagaa
      301 aattcggcgc gcggccgaac tcttcgtcgc gcgccggtgc gggcggttct tcgtcatcgc
      361 gcccgctgcc gtatgcaacg caaccggcag caacaagcaa tggcgacgct ctgcgcttca
      421 tcccgctcgg cggactcgaa gaagtcggcc gcaactgcgt gttctttgaa tacaaaaacg
      481 agattgtcat catcgatgcc ggcctccaat tccccgaaga agaaacgccg ggcatcgatt
      541 atatcatccc gaacgtcgcc tatctggaaa aaaagaaatc aaacatccag ggcatcatcc
      601 tcacgcacgg acattacgat cacatcggcg cgctgccgca tatccttgaa aaactcggca
      661 atcctccgat ctatacgacc gcgatgacga aagcgatcat agaaaagcgg cgggacgatt
      721 tcccgaattc gccgaaaatg aacatccagg tcgtgaagaa tcacgacgaa ataaagctct
      781 cgaaccattt caccgcgctt tttttcggcg taccgcatac cattcccgac gcgaccggcg
      841 ttctcttgaa aacgcccatc ggcaatatcg tccatctcgg cgatttccgc gtggactatg
      901 acgaagaggg tgttgcgcag gggcttgacg aattcgaaaa gatcgggaag atgggcatcc
      961 acagttttat ggcggaaagc accaatgcgg aaaaacccgg ccattccgtt tcggaaaaaa
     1021 tcgtcgagaa aaatctcgag acgattttca aagccgccaa agggcgcatc gtcgttgcga
     1081 ctttttcgtc acttcttaca aggatcgccg aaatcataaa aatctcggaa cggctcggga
     1141 gaaaagtggc gttctccggc tattccatga aagcgaatgt ccagatcgcc cagaccctcg
     1201 gcttcatcaa agcgaaagaa gggaccattg tcccccttga agaggtcagc aaactccgcg
     1261 acgacaaagt gatgatcctt tcgaccggcg cccagggcga atcgaatgca agcctgatga
     1321 aaatcatcaa cggcgaacat cgcaacctga aagtgaagcc gggcgacacg ttcatcctct
     1381 ccgcgtccgt catcccgggc aacgagcgga gcgtacagta tctgaaggac aacctcgcac
     1441 gccagggggc aatcgtgcat caatcaaccc tgatcgatct ccatgcctcc ggccatgcgc
     1501 tcaaaggaga tatgcagatt atgacgaaac tgatcaaccc gaaattcttc attcccatcc
     1561 atggctacta tttcatgcgc gcggcaaaca gcatttcggc gcaagaggtc ggcatcccga
     1621 aagaaaacac ttttcttatg gacaacggcc aggttgcgga aatgacgaaa accgctttta
     1681 aaataacgaa ggagaccgta ccggcatttt acgtcatggt ggacggcctg ggggtgggcg
     1741 acgtggaaga agtcgttctc cgcgaccggc gggcgctggc gcaggaaggc atgctcgtca
     1801 tcatcatgac gctcgaccac gacaagggaa aacttcttaa aaatcccgac attatttcgc
     1861 gcggtttcat ctatctcaaa gagaatcagg aaatactcga agagatacga aaacggatcc
     1921 gcgcgctgct cgcgcgactg ccgaactacg acaagcccga tccggattat ctgaaaacgc
     1981 tcgtccgcga ccaggtcggc cagttcctct tcaataaaac ccgccggagg ccgatgatct
     2041 tgccggtgat aatagagata taaacatacg agcgacatcc aatgtcgctc gtattccgtc
     2101 gtcctcgcga aaacgaggat ccacgctccc aatcttctct ttttgaattt taaaagcatg
     2161 gattttctcc tccgcgggaa tgacaaaaaa attatgttaa aataaactca tgaagccaat
     2221 cttagtttct ggcatccaac cgagcggacg attgcatatc gggaactatc tgggcgctct
     2281 aaaaaatttc gtcgacctcc aggattccga cgaatatcag tgttattttt tcatcgccga
     2341 tctccattca ctcacggaac cgttcgatcc gaaagaaaaa caacggcaga tactgaatct
     2401 taccgctgat tttttggcag ccggaattga cccgaaaaag tcgactatct ttcttcaatc
     2461 gcaggttccc gcgcacagcg aattaatgtg gatattaaat acgatcacgt cgatgggaga
     2521 gcttttgcgt atgactcaat taaaagacaa agcgccagaa acaataaaac agataaaaga
     2581 attaatcgct aagaatatga gcgatttaga ggccattgcg atacgggaag atataaatga
     2641 aacacaaatg aatcttacca atgtcggtct ttttgattat cccgtgctca tgacggccga
     2701 tatcctactt tacggcgcca atatcgtacc ggtcggcgac gaccagctcc aacatctcga
     2761 actcacccgt tcgctcgcgc gtaaattcaa tacaaggttc ggcaaaacct tcatcgaacc
     2821 gaaagggtta ctcacaaaaa ccccgcgtgt tatgagctta aaaaatcccg agaaaaaaat
     2881 gtcgaaatcc cagccggaaa gctgcctctt catcgatgac gaccccgaag atatacggca
     2941 aaaaatcgcc cgtgccacga ccgactccgg ctcggaaata atctatgatc cggaaacgaa
     3001 gcacggcctc agtaatctct tggatatatg cgccgcaatc accggaaaag aagtttcaat
     3061 gacagtaagg gaattctccg gaaaaaatta ttcggagttt aaaaaccaac ttgccaaaat
     3121 agtatcagat cacttcgccg ctttccgtga aaagaaaaag aagttgcttg cctccgataa
     3181 aaaattgaaa acaattcttt ccgccggctc caaaaaagcg gcggatgtcg ccgataaaaa
     3241 aatggcggat gtgaaaaata agatcggcat cgcgctttaa aaaatcgtcc gagccgtgga
     3301 tccgccgaga ttcgcacccg gttccctgtt tggcaaaagc gggattttac atgcataaac
     3361 tacggacccg tatccacggc tccaaaataa taatgaaaag agaattaaaa atatcttaaa
     3421 aacagaaaca gccacaaaag tggctgtttc tgcgccaaac gaggatttaa agtcggcagt
     3481 tacgcagact ttcccgcttc gttattatca taatcctcac atctaattcg tcaattctta
     3541 tttaattatt ttttaacctt taatttcgcc gattatccaa tcaaaacatc cttccttgca
     3601 cgcttttcgg ttttgaatct tccgccggct cttttccatt cgcgataaaa cgcttcataa
     3661 gcgttgtaaa tccttttttt tcgaaatagg cggtaatgac ggattcgtca taatccggcg
     3721 cgagcgtgga aagcgccacg tcgcccatcg gaacattttt ttcaagcacg accagttccc
     3781 tcgaaagatc ggcttccttg cggaacggtc ctaatttttt ttcgagcttc ggatccgatc
     3841 cgagattttt ataaatattt tcgatgctcc cgaatttttt taaaagttcc gtcgctgttt
     3901 tcggccccac gcccggcact cccttgatgt tatccgacgc atcgccggca agcgccttat
     3961 aatcgatgat cgcgcgcggc ggcagtccgt atttttccat caccgttttt tcgtcgtaga
     4021 tcgtcgtatc gctcacgccc ttattgaacg tctgcacgac aactttatcg ccttccacga
     4081 gctgaagcgt atcgcggtca ccggttaaaa tgacgacacg caaatctttt tcgtctttga
     4141 atttcgtcgc gagggtggcg ataagatcgt ccgcctcgaa tcccgccttc tcgaatgtcg
     4201 ccacgccaaa cgcgcggaaa agattgtgca tttcgataag ctgcgcaatg agaatatccg
     4261 gcgcgggcgg gcgctgcgct ttgtatgcgg catatttttt gtcgcggaac gtcggctcag
     4321 gccggtcgaa aagcgccgcc gcataatcgg gcctgtcttc gcgccataat ttcaaaagaa
     4381 tgctcgccac gccgtaaata gcgcctgtcg ggcttccgtc cggcgcggta aacggcggca
     4441 acgcgtgaaa cgaacggtgg atgaccgaat tggcgtcgac aagaaggagt ttcttcatgt
     4501 tttatttctg tcgtccccgt ctctcgaaaa cgctggataa gctccggcga ggatttatgc
     4561 tttaaaattt aaaatcgaaa gcatagatcc tcttcttcgg aggggcgact gccttatatt
     4621 attatttctt ttataattct ttcgttttct tttttgccgt accggaacgc cagctgatgc
     4681 gcgccgcgcg ggataacctc ccgcgcattc tccggccatt cgataaggat aacattcttc
     4741 gggtcggata gtatttcctt aaacccgaga tcgacaaggt cttttgtttt cttcagccga
     4801 taagcgtcga catgataaat attctcgatc cgcgatgtac ggttcacgaa cttcggatcg
     4861 cgtaccttaa acctcctgaa aataacgaag gttggacttt tcacgcggcg cttgatgccg
     4921 aacccgcgga aaaatccttt cgcgaacgtc gtcttaccgg agccgagatc gccgcgcaac
     4981 gtaaaaaccg ctgcggtttt tccacttttc actttcttaa agcgtgggtc ctcgcctccg
     5041 cgaagacgac acaataaaaa ttgtgccgat tttccacttt tcattttttt ggcaagcttc
     5101 tcgccaagca actcagtttc tttcgaagaa aagctgtgat aggttttcat tttctttaaa
     5161 ggcgtacatg ataagtcccg ccataagcgc ggcggtgccg acaagaagcg aggcgaactg
     5221 cacgccgctg attgacgtcc ggttaacggg aatattgaac ggatggtttt ccgacgcgat
     5281 atgaatttgc aataccggat cattcgcggc gcccggcgtc ggatcgccga aggcgaaatg
     5341 ctgcgacccg tccgtgccgt aattcacgcg attgaaactt tgcccgcccg cggcaccccc
     5401 cgaaaaactc gactggtcta ccaatacgcc gttcatatta tagagcgaca atgtatcatc
     5461 gctgttcttg agcgtcagct tcgtttcgct ccgcggaagg acaagatacc cgcccgccgc
     5521 aagctgatgc cccgaaagcg aaaattgttt cccgttcgcg gtcgcgatct tccatccgtt
     5581 caaattcacc ggcgcgccgc cgttattgaa aagctctaca aattcccctt tcgcgtccgc
     5641 tcccgccgga ttcggcagcc attcattgat gaagatcatg attatttaaa taataccagc
     5701 gttaccgtta aaaattaata aaatatccaa aaatcttttt gttatgaagt tctgtatttc
     5761 tgtcgtcctc gcgacggtga ggatccatgc ttccaatctt ctttttttaa tttcaaagcg
     5821 tagatcctcg accggagtcg aggacgacag agaggagccg ctttttgaat tacaacaact
     5881 cttccttccc ttttatgcca agatgcttat atgccgccgg aagcacgatc ctgcctgatg
     5941 acgaccgctg gatgaaaccg agcgtcatga gatacggctc gtaaatatcc tcgatcacgc
     6001 cccgctcttc cgaaagcgcg gcagtcagcg tattaatccc gaccggcccg cctttgaatt
     6061 tctcgatgat cgtcatcaag agccgccggt cctgcggctc aagcccgacc tcgtcgatct
     6121 caagcatggc gagcgtctgc catgccgcat tttcgtcaat cgctttcgtg ccgcgcactt
     6181 cggcgaaatc gcgggcgcgc ttcagaagcc ggttcgcggt gcgcggcgtt ccccgcgacg
     6241 cgcgcgcgag gattgcgacc gcttccggcg tgatatcaat gccgaaaagc cccgccgagc
     6301 ggttgaatat ttttttaata tcgtcaatat cataaaaccc gagccgcagg gtcgcgccga
     6361 accgcgaacg gagcggctgc gacaaaagat tttcacgcgt cgtcgcggcg atgagcgtga
     6421 acggcggcaa gtcgagagaa agcgtccgcg cgccggggcc cttgccgacc acaagatgga
     6481 gcttccgcga ttccatcgcc ggatataaaa cctcttcgat catcggattg atccggtgcg
     6541 cctcgtcgat gaaaagaatt tcgccggggt tgaggttggt caaaaccgcc gcaaggtcgc
     6601 cggttttctc gatggccggc cccgaggttg tcttgatcat cccgccgagc tcccgcgcga
     6661 caagatacgc gagcgtcgtt tttccaagtc cggcgggacc ggaaaataaa agatggtccg
     6721 ccgattcgtt tctcgctttc gcggcctcaa gaatcacccg caggttgttc tttatttttt
     6781 cctgccccac atattcatcc catgcggccg ggcggagcat gctgtcgata ttctggtcgt
     6841 ccttgttatg gttttgattt gctttcttca tgtgtggtat aatgtacttc ccgccgttta
     6901 atgatgatgt tcctgcttcc tgcggctaca acgcttcgtt tcaagagaac gccggaagga
     6961 cgtactgtgg ataaaatggg tattgcatgg gacttgacaa gctattttct ttatgctaac
     7021 atcccctaag gatcgggttt tggttctttc aaatctgggg gcggaattat ttctaaacac
     7081 ttcaggacgg atcggttccg gctgatccta tcctttgaat aattctaacc tcttttatct
     7141 tttgataaaa gagttcatgc aatcccttct atgaggggaa ttgcccccag gtttgagaga
     7201 ataacatcct taaaaacggc cgccagccgt ttttttatgt acgactcaaa aagtgaacat
     7261 gcggcgtcac cacgctatct ctcccgtcgc gcccacgacc tcgtcaaacg tcgtcgtacc
     7321 gatcaacact ttcaaaatac cgtcctcctg catcgttatc atttcctggc gcttcgccac
     7381 ctcgcggagc gcgacctcgg acgcttcttt caaaatagtc gtttcgatat ccgccccgcc
     7441 ttccagaaac tcgaatatcc cgatgcggcc tttgtagccg ataccgctgc attggtcgca
     7501 acccgccgcg gcagatacgg catactgttc gtatttcgat ttatcgattt tcgccggaag
     7561 ccgttcgaga aagcttttta ttttttccgc cgtcgccgca tcgggcggcg acggtttttt
     7621 gcaatacggg cacagcacgc gcaccagccg ctgcgcgatc acgagcgtga gcgccggacc
     7681 gatactcacc ggcttcacgc cgagattgat aagccgcgga acggcgccga cggcatcgtt
     7741 cgtgtggagc gtcgaaaaaa caagatgtcc cgtaagcgcg gcctgcaggg cgatatccgc
     7801 cgtttcaaga tcgcgcacct cgccgaccag aataacgtcc ggatcctgcc gcacgatggc
     7861 gcggaggccg ttcgcaaacg tataacccgc ttcgtcgtca acctgcgtct gctcgacgcc
     7921 ttcaatctga tactcgatag gatcttccag tgtgatgatt ttctcctcgg ggttattcat
     7981 gttccgcaaa aaagcataga gcgtcgtcgt cttgccggaa ccggtcggac cggtgtttaa
     8041 aataaggccg ttcggctttt tgaggttttt ttcgacaagc gttagattgt cctcgcggag
     8101 accgagttcc ggcagtccca ccatcgtcgc cgccggatcc agaatcctca ttacgatcgt
     8161 ctcgccgaac tccgcgggaa gcaccgaaac gcgcatttcg acctccttgt ccgcaagttt
     8221 tatggaaaac cgtccgtctt gcgcctcatc atggacattc agcttcatcc ccgaaagcag
     8281 tttgatgtgc gatataaaat tcgcgtacgg cgccagggga aattcgtcga aaatatcgtg
     8341 cagaagcccg tcgatgcgga aacggatttt cgctttcttt tctcccgcct cggtatggat
     8401 gtccgaggcg cgcatcgcga gagatcccgc aagaattatt tcaacgatga caaccaccga
     8461 cgtttttata aaatcaatgc ccgcaagctc gtttttgcac gcctcgatgc tggtgaggcg
     8521 cgcccgcaga tcgtcgagcc gcgtcttcgt aatgctcact ttgcccgtga tattttccgt
     8581 gacgggcttc acgaatttat aaaaacgcca tgccgaggcg agtcccgacg gcgaaacgac
     8641 gaaaactttt acgtcgtatt ttttatccct gagatccgcg ataatttttt ttgtcgcggg
     8701 aaggtccgga ttcacggcaa caatcgcaag ttgtctcgct cttatctcaa tccccgccac
     8761 cttcccgtcg cgcgcctcgt tttcggaaat caccttcaca gcatcgaggg acaccggcat
     8821 ttttccaagg tcggcataag gatatccaat ctttacggca agctgctgcg ccatgcgctc
     8881 ctccccctcg cgcctcaact tttcgatctt cgcccgcaga tcgtttttgt tcggttcgtc
     8941 ggccattgag aatattatac cgtaattttt tcttttatgt tgcggcgtgt tgatttttcg
     9001 tcggcatcgc ggctgtaagg atgcgcactt tcccgtcgtc cgcgcctccg cgaggacgac
     9061 ggaggggagc gcatgtccgc taaattttta tttttattca accgacgcta taataaaatt
     9121 atggcgggac attcgcattg ggcgggaata aagcataaga aagaaataac cgatcataaa
     9181 cgcgcgaagg ttttttcaaa actgctcgcc gcaatctctg ctgccgcaaa agccgatccg
     9241 gacccaaatt tcaatccgcg cctacggaca gccatcatga cggcaaaaga actggccgtg
     9301 ccgcaggata atattaaccg cgccattacg cgggcgaaag aatcaggcga gaacatcgag
     9361 gaacttgttt ttgaagcata cggaccgggc ggcgcggcaa tccttatcgg cgcgatcagc
     9421 gacaataaga accggacggt gcaggaaata aaactgatcc ttaaggaaca caacggaaaa
     9481 tgggccgaaa ccggaagcgt gcggtgggcg tttgaacctg ttgcgaaagg gtcggatgaa
     9541 tggaaagcaa agttccctgt cgcaatttcc gacgaagaaa aagaggcgct ctccgtcctc
     9601 gttgaagcgc ttgaagaaaa tgaaaacgtg caaaacgtat ccacaaacgt agaatatgga
     9661 aaagagaaca gggcataaaa aaccgcacct gcgccccgta ccgcattctg atttctatat
     9721 tcttaattct aaattctcta ttcttaattc ttatgattgc attaggtatt gatcccggaa
     9781 cgcggagaat cggctatggc gtcgtccgca agaacggcag cgaaattgtt tttgtcgccg
     9841 caggactgct tgacataaaa agcagcgacg acatcgcggc cctccgcgag acaaaaaaag
     9901 aaaccgaccg gcttatcaaa aaatacaggc ccgacatcat aggcgtcgaa aaactgtact
     9961 tcgccaaaaa ccagaaaaca gcgcttgcgg tcgcccaggc gcgcggcgtc atccttctct
    10021 ccgccgccga acaaaaagct ttcattaaag aatatgcccc caacgaagtg aaggcgggta
    10081 tcacgggcta cggtttcgcc gataaaaaag cggtgctgaa aatggtaagg ctcattctcg
    10141 gaaaacgcga ccttgcggtc atcgatgatg cgagcgacgc gctcgccatc gccatcatcg
    10201 ccgccggaga acgcctttta taaaaaccgt gtaattttta caatgcggcg cttccggaat
    10261 gggccaagaa tgtgccatcc ttcgcggata ggaatgtgga gtcccttgac aatcttccgc
    10321 ggtaaaatat actggtctta ataaatttta gaaaaggcag atttttcttt tttaaaggtc
    10381 gcttcatatt tgaaaaaaca tgacgcttgc gctggaaaaa ttaacactca aatttcgcgg
    10441 attgagcgac gaagaagacg aagatgcggg gctcatcaat gatgcccctg atatcgaaga
    10501 ggaagagatt gaagacgaag acgaagaact gtcgggcggc aagaaaaccg acgacgatgc
    10561 ggacgcaatg ggcgacgaag gataaaagta acacgataaa aaaaccccgc caaatgacgg
    10621 ggttttttta ttgctaaatt gcgcagatag ctttattttg ataccatcaa aatataaaaa
    10681 tgaacgaccg gccgcgcagg aaaatatggt tttggctgca aatcgcggga atcgtcatcg
    10741 cgtctctggc gataattttt tttatcgtcg tactctactt ctcgaaaacg ctcccctcga
    10801 tccaggaaat aaccacgcag caaataacgc aatcgacgag aatctacgac cggacgggaa
    10861 aatatcttct ctatgaaata tcgagcggcg gaaaccggac cgtggcgccg ttcaaccaga
    10921 taccgcaaac gctcaaagat gcgaccgtcg ccattgaaga ccagaatttc tataacgaac
    10981 ctgctttcga tataaaaggg atcgtacggg cattctttgc cgaccttata agcggcggca
    11041 tcgtgcaggg aggatccact atcacgcagc agcttgcaaa gaatgccttt ctcaacctca
    11101 accagacatt ctcgcgaaaa ctcaaagaat tgatcctcgc tataaaactg aaccggtatt
    11161 attcaaaaga ccagatttta acgctttacc tgaacgaaat accgtacggc ccgaatattt
    11221 ccggcgttga agcggcgagc gaggcctatt tcgggaaagc ggtggaaaat atagacctcg
    11281 cgcaaagcgc cgtgctcgcg gcgctcccgc aggcgccgac gtactattcc ccgtggggaa
    11341 gtcacgtgag cgatcttttg gcgcgccagc atatcgtgct ccagaaaatg tacgccttgg
    11401 ggaaaataag caaaacccag cttgacgatg cgctcgccga aaagatcgtg ttccagccgc
    11461 gttcctcaag cggcatcaag gctccccatt tcgtaacggc ggtccaggat tatctcgtcc
    11521 agaaatacgg cgaggatatg gtcgataacg gcggcctgaa agtcatcacc acgctcgact
    11581 ggacgctcca gcaggaagcc gaagccgacg tcgccgcggg agtggcccaa aatacaaaac
    11641 tgtatggcgg cacgaacggg gcgctggtcg ccgaagatcc gaaaaccggg cagatccttg
    11701 ccatggtcgg ctcgagcaat tatttcgaca cgaaaaacga cggcaatttc aatgtcgcga
    11761 cgcagggatt gcgccagccg ggatcgtcat taaagccgtt cgtttacctt ctggccttcc
    11821 agcagggata cgcgccgcaa accgtccttt ggaacgttcc cacggaattc agcaccgatc
    11881 cgtcatgtcc ttccattccc gattttgcga gcaccaataa aaaatgtttc cacccccaga
    11941 attatgaagg gacgttcacc ggaccaatca cgatgcgcga cgcgctcgcc cagtccgtca
    12001 acattcctgc ggtaaaaacg ctttatctcg tggggcttga tgccgccgtg gcgaatatgc
    12061 aaaatttcgg tcttaccacg ctcaccaatc ccgatgccta cggcctttcg ctcacgctcg
    12121 gcggcggcgc ggtgcatctc attgatctta tgaaggcgta ttccgcactc gccgccgacg
    12181 gcataaagca tgaccaggcg atgattctca gcgtacagga cgccgacggc aacgtccttg
    12241 aatcgtatac cgaccacagc gaacaggttt ccgatccgca gtcggtccgc ctcgtaaacg
    12301 acgtcctttc tgatgtgaaa gcaagaagtg gcctttttca aaacagctta tcccttaccg
    12361 tctttcccgg ccatcaagtc gccctcaaaa ccggcacttc gaacgattat cgtgatgcgt
    12421 gggcgatggg atatacaccg tcgctcgtgg tgggtgtttg ggcgggcaac aacgacaata
    12481 cgccgatgca ccaaaacgga tcgagtatcc tggcggcagt gcccatgtgg agcgcattta
    12541 tgaaccaggc gctccaaaac gcgccggacg agacattccc gagccccgcg cccgtcaacc
    12601 cgtcaaagcc gttccttgcg ggagattata tcgaaaacaa ccaaatccat tccgagcttt
    12661 attacatcga caaaaacgac ccgaccggcc ccgcgccgac agatccgagc gccgatcctc
    12721 aattcaaaaa ctgggaagcc ggcgttacgg catgggcaca gcaaaacgga atacaaaaaa
    12781 cattacagga cgcagcgctg aacgcgacct caagttactt tcctgaaata agataagaga
    12841 gggttagccc cctctacctc tcctccttct cagcgaggga gagatcccaa tttctcccct
    12901 ttccaatagg aggtgatgat gggttcccgc tcggaccgtc cccaaccata attaagggct
    12961 gtgatgatag gatgaaaatt gtgtcg
//
LOCUS       DAIDDT010000023        12376 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_23_length_12376_cov_6.532726, whole genome shotgun sequence.
ACCESSION   DAIDDT010000023 DAIDDT010000000
VERSION     DAIDDT010000023.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12376)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12376)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12376
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23_length_12376_cov_6.532726"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3249 a         2751 c         2913 g         3463 t
ORIGIN      
        1 gttttttgga attatattta tttacgtaag cataagatcc tcccgttcga tcatcggttc
       61 ggcttttaag aaatatcacc gttggatgac tgccggagcg atatgtttta cgttcggttt
      121 tttattggac cccgatgtta ccggattttt tgggatgaac agcaccccaa gcgagggcgt
      181 gcacgatctt tttcttttat tcgcggtgat aatttttgtt atcaccaatc tttatcttcc
      241 caaagaagcc tcggagtata tgaatataca caagaatgac aaatagcgtg cggccggttc
      301 gctcgttgaa aataaaaata ttctgtgggc acactagggc ctgaaccagg aaccggacgt
      361 gtagagccgc aaggcggata aaatgggttt ggtcgtcgac tactgcaatg tgcgcccgcc
      421 tggactcgaa ccaggaaccg atcgtgtata agacgattgc tctaccattg agctacaggc
      481 gcagcgctct gtctgtcatc gtccgcgaag gcggatgacc catgcttccg atcttaattt
      541 ctcaaagcgt gggtcctcgc ctgcgcgagg atgacagaat agagaagatt aatatcctgt
      601 tatttcgccg tgattgcgtc gaactttgcc atggattgcg tcgcctgcgc gttgaaatta
      661 ttgacggcgg cgacatcggt attgattttg ttaacaagcg cttgtacgct gccggcggtc
      721 gtcggctgtc cgacgaaccg gttgcggagc gtattaagca gtgcatcgag atcgttgctg
      781 taatttacga gctgatccac gagcgcgagg cggtttgtga tggcatcaag cgccgcctgg
      841 cgggcatcct gcgaattgat tgatgaaagc gcctttgcca tgtcgccgac ctgcgtagaa
      901 agcgcgatgg cctgattgcg gatctcctgg ctttgggcga caagctttgt cgtgagggtt
      961 aacgcgtccg tataatcgcc gcttttatcg tattggttaa cctgcccaag atcgttcgtt
     1021 gattgggtgg aaagcgttac tatattttcg gcgatggccg ctccctggag tcgcgcatcc
     1081 gtgaaatcct gcggaacccg gttgttttcc ccctgtaaaa atcttgcaac gccgtagccc
     1141 gtggtaacga gaataaggaa gacgagaatg gttttagtgc gacgggaaag gtatttcatt
     1201 atttcatagt tttaacagat gacggcgaaa tatgcaagcg cgcggatgcc gtttgaaaag
     1261 ttatttttgg gacgcgttac ggctgcggtg ttttataata tacgagagtg tggaggaaat
     1321 gggttctttt taaatttccg ttctgcgggc ttgagaagtc gcggcgaaga tattactata
     1381 agaaacagaa tgggcgttac ggggaactat gcgggtatcg tataacggta ttacctcagc
     1441 tttccaagct gatgacaggg gtccgactcc ccttacccgc tccaaatagg aagttgctgt
     1501 agcgaagtga aaaattgagt agtgttcatc tgcatttctt gaggctctaa cctcgcaaat
     1561 tcccgttgtg cttccggcag acgggtaaag atcatttcga agggttttcg taaggtaata
     1621 tctatcttct ggtcggacaa cacgaggctc gaaccaagac aggcaaaaat cgcgcggcgc
     1681 atatcgtcgt ctcctttcgc aaaccaaaca ccggcatatc gggcgaaatt aaatgtccgt
     1741 tcgctcatct cgagccattc ttccttttct ttgccctgca cattgagatc gtcttccaat
     1801 tttgattttt ctttaagcaa tgccgatttc atccggctgt attcttgcgc gttgatgtat
     1861 tcgccgtcgc ggttttcggg cgaggcatac ttgaacatca aagcgtcgag ctgttggata
     1921 atcgtaacca attgcttctg tttgtcttca agcagtgtct tgtaactggc ggcttcctct
     1981 ttccgcaaga agtaaaggtg cttgatcgcc cagtccttga acttttcgga tatcgtaatt
     2041 ctctgaatca gggtgtcgat ctgcttgttg aattccttaa gctcgacggc tttctccggg
     2101 caatgcttgt ctctctttct cgtgcagcgg taatagacgt agcgatgcac attgccgttc
     2161 cgctgatatt tgaatttttc ttccgccgtg atcgcacaac cgcacgatcc gcacttcatc
     2221 aagcccgtaa aggcgaaact tttggtatgc gggcgcggct ggctcggctt gcccaagaat
     2281 ctttggatct tattgtattg atcttccgtg attatcggcg ggtgcttgcc cttcagccag
     2341 ttttcgctcc ctttcggcca ttcgtaccag ccgtaataga aaggattgcc gaggatgcga
     2401 taaagttcgc ttaagtgaag ttttcgaggc tgacggccac gcgtcatggg catcgtgagg
     2461 ccgatcttgt tggcgagctc aagcaatttc ggagcggtat agtttccagt aagcatatga
     2521 ttgagtaatt gcttcaagag tccgaagcgc acgcgatcgg gcgcgatgtc ctgctcgccc
     2581 ttgagcttcg tcttgctgtt cacgtaaccc aacggcgcgc ggcccggacg ccagcccgtt
     2641 tcggcttttc ggttcattcc gcgcttcacg tcgttgctca atttgtccga cgaatacttc
     2701 gattgcgaaa gcgtgatctg gagcatcatc ttgccttccg gcgaattgtc gaaatagtag
     2761 gaacagaatt tgaggtcttt gatttttcct atgtcgagca aatggatcac ttccgcggcg
     2821 tcgatcggat tgcgagataa gcggtcggga tgccacgcga tgatgccttg cgcttcgcct
     2881 ttccgaatgc gcgcgatcat gtccgcgaag atgggccgct tcttcggctc gaatgcgctc
     2941 acggattccg gcgggagttt aatgatcttc aaatcgccga agcgtttgat cgcttcgtcc
     3001 gtttgagaat ccaaactcaa cacctgccga tcgccttcgt cggtcgattt tcggcagtag
     3061 aggaaatagg tcggttgttt tgtcagtagt cctttgagca ttcctttttt acagtaagcc
     3121 acggggcgag attttcaata cgggtaatgc ctcgacgttc gaactcgtct atcgcatcga
     3181 gaaaccctag cgtttcgggc atgatgccat gcttttccaa atcctccagc accgcgacta
     3241 tatcggcttc ggtgtaatac ttcaagagga tcacccgcgt cgtggccaaa aggcgcttga
     3301 gcgttttgct cgagaacatg cgctcgtatc cgtttgtgat gctagactgt tccattggtt
     3361 ttgtgattgt tttcgaattc ttccgcccat tcttttctga tcgggcggta gatcgcgtcg
     3421 aacatcgtca aaagcgcggt cgcttcttca agcgcctttt cgtcggagat gtcttccccg
     3481 aattcctccc gccaaatcgt tttgaattct tgcaatgctt tttcggatac catgatgttg
     3541 gcgccacagt ggcgtcaact taatgatagc ggcatgttca aaaatggagc cggaagccgg
     3601 ggataacttt acaaatatat ttatatttgt ggcgcggttg ctctatatat tatatttgcc
     3661 cctatttacc tgcatttccg ttatccacac atacgacggg aaaagctatt gacggctttt
     3721 gcgcgaaggc aaaattattg aaaaaaatgc tacaatttaa atatgcaaaa ggagggtgat
     3781 ctggggacaa aaggatggta tcgagcccta aaagttttct acatcatagc gaatattatc
     3841 ggactgcttt tggtagtcat tcttgcatat tctttggctt acacgagccc attccttttt
     3901 ctactatacg ggacattaat agttttgatt ttgctccgct tagtaagatc aattttctta
     3961 tacgtctata acgccacccc attttggaaa agttttttgc caaaagcgtg gctgattaca
     4021 atcggggtta tcgttttatt aaccattctc gctgcagtcg ggaatatgga aaattcgtcg
     4081 agtcctagta ccccatcgac ggcaaattcc ggcacaaatt caaattctca aggatatacc
     4141 ttgcagcagc tacaacagat gggggcacag ccggttgaac caactcaaaa ccaacaaccg
     4201 agcacttcca atcccacttc acaatattcc acttatacca acgttcgatt ttcctactca
     4261 atttgttatc cgacaggaat attgattcca caaggtgaat ccgcgaacgg cgacggccaa
     4321 caatttcttg ctcaaaatgg ggcggcgaaa gtccttgtat atggatcaaa cgccgtaggc
     4381 gaaacacctc aacaagaatt gcaactcgat gaaaactatt acctcaacaa taattcgctt
     4441 tccacaatta ccgatcaagc aataaatcaa acttctttta ccttctccgg atcatcgcca
     4501 tcggtagctt tttatgaaaa aactttaatg caaaatggac agtataaaac tttttccatc
     4561 caatataatc agcaagcagc ttcaatattc gccccgatag tatccgaaat ggtgcaatgt
     4621 ttcgcgaata cacaatagca ctgacttcag atggagaaag cctcgttcaa tccgagcgtt
     4681 gaaattcatg cgctctgcgg gatagcaaga acggatttaa ttgcggcgcg tatcctctgg
     4741 aatatggggc ttattgcgca ggccatctgg ttagagcaac aaagcgcaga aaaatatttg
     4801 aaagcgttat gggcaaaaac agcaactttt accagtgaaa acaattttaa aaattgccta
     4861 cggcaattcg gcagaaaaag cactacgcat ggcatcaaag aagtttttga agcgctacct
     4921 ttggatcaga aaaaacaaat caaacacgga ttgaatatcg ttgccatcga tcctctacga
     4981 tatgaagcat catttggatt cagttatggg gcttttcgat ctggcgaact atttatcgcc
     5041 gatgtgagaa aactgctagg agaaaagccg gttagccatt tgaaagaatg ggaaagatcc
     5101 gcaatgcgtc taaaaggttc tcctaaaatg gaaagtaaaa tgagacaagc tgcaaagtta
     5161 atctattcaa ttacgaatcc taaaacgaaa aaagagcagg ctcaagaaag gagacgattt
     5221 agaaaaatta tggatgatat agcgggggaa atctttgcac cccgagttta aaattgaggg
     5281 gttatttccc gaccgcccaa cgcccgtatg tccgcggtac gggtgaaccc ctcaatttta
     5341 cacctatgcg tcaaatttgt ccggttaaac ctaaatatcg aacgctataa cgccatactt
     5401 tttgagtttt tcccgatagt tgggatatcc gtaataaatt tggcgcattt caggcatcga
     5461 ctccactgac ggcatgattt tctggcgtgg tattaccttg accatcgcgc cgatcgattt
     5521 gaaaattcga acccgcttga ccttcttctc tagccgattc ttgccgcaaa cgataacgag
     5581 tgtatcgccc tttttcacct cacaatatct cggtgtcgca gcgcgagttt caaccacctt
     5641 caagccatcc ttaatctcca aaaaattatt tttatcgacg acattaaatt ttaagataaa
     5701 ttttttcatc ctgtgtcgat aatttcgttt aatagttcag ggaattcatc cgttcccata
     5761 tcctccatcg tgaaatgtcc atggcctttg atttcaacaa ctttaccgcc aagcgcactg
     5821 tgaaacatag ctaaacttct tttaccgttt tcgtgttcgt tatctgccgt gaacataaca
     5881 attttcttaa cgcgatcctt aatcgtagga tcgacggtgt aatcgtaaaa taattttttc
     5941 tcattattgc tcgttcctat tttccacggc gcgaccaaaa tcagttcatc tacttttttc
     6001 ttcgcatctc caagccagcg taccagaaaa gcggcgccac aactgtgtcc gattaggatc
     6061 gtatgttcat taatatcttg tttttcaaac tattttttat attcttcata gttcggtttc
     6121 cacggcgtag gcatcaacgg tatagtcgta tcaatgccac gcgtattcaa agattgctga
     6181 atccaaggaa tccaatgctt atcaaaagtc atatcggttg gcgacgaggg gcatccgtga
     6241 ataataacgc aattctgcat ggctacttta ttacttcgct aattttattt ttattgtttt
     6301 caaaaaatgc gggtgcgccg gaatggagcg gcgagggaag gctatcaagc ttgaaccagc
     6361 cgatctcgtc gaatttatgc ggctcgccat ttttcacttg atcgtggtcc acacggacga
     6421 caaaatcgag tgttagccag tgcgtctttt taccatttat ctcgcggaaa agatcgcggt
     6481 atcccatgaa tttataatca aggacgtcgg cgcagtattc ttcctttatt tcttttttca
     6541 gcgtcgtttc aacgctatcg ccgaattcta aaccgccgcc gcctggatcc catttgccgt
     6601 tttcatcccg tgcttttgcg cctcgtttgt tcaataaaac attgccatgt ccgtcgtggc
     6661 aaagatagca gacggtaata ccgatgtgat cgaagccttt ttccatgcta aaattatacc
     6721 cgaatttaac caatcggaaa attgagcttt tcttcgggaa atcctattga aacactaatc
     6781 cgccccaatc gtctcggcga cgtaaatgac atgttgcttg ttcgcagtga tcctctctgt
     6841 ccgttttcca tcttttccat cgacatggcg aacgaatgta acctcgccgc ctttctcctc
     6901 gagatcctcg gcgtgcatgg gaaaattcat gccagaaagt agaatccaaa tctcgccgat
     6961 cctatttcgg cgcctcgctt tgatgttcat cggctgtagc ttgatcatgg ttttctttta
     7021 tgcttgattt tcgacctttg ggctgatcgt cggggataag atagtatccg cgccgataca
     7081 gacgtaaccg ataattgcca tacttccggg cgtagtgctg cgataaatct ttcttcgata
     7141 cgatctttag cagatacaga ttgcagattt cattcagaat ttgatcctct cttttctgcg
     7201 ccatcgcaag caccttccag atcgtgggct tggcttttgc ccgtgccacc caccgagaaa
     7261 gattgatcgg gctttcctcg ttttccctta aatgttcttc tctttctttt cttatatggc
     7321 ttttctcttg ctttgtggca agaaaagcca atagattact tttagtaatc tgtagcggtt
     7381 cctgtagatt ttgtgtttcg gccgtaagcg tttcgagata cgaaagcttg tttcccaaga
     7441 tcacggaaag ctcggcgatc aggcgatcgc cgccttgcga agcgaattcg gcatgatcgg
     7501 aaacggataa agccttgccg cgttcgatag cgccctcgat tttctggatt tcatcctcca
     7561 attcccggac gggcgcttcc atttccgtta tttgcctttc tatcgcgtcg agatcggccc
     7621 ggcactgccg tatagtgtcg acgccggaaa acgcctcaat tttcgaggtt tcagacaatt
     7681 gaagcatggt cgcgcgataa tttggggatc gaaaaccacc cacccgtggt tttccagtag
     7741 gctctaatgt cgtggactaa cccgctccag tttcaaacaa gtttcatttt cccaaaaatt
     7801 tttgcgcgct tcgcggagcg aagcgtaagg tggggcggga gcaaggccga cggggtatcc
     7861 gccggcattt ttgacgggtt ctattgcgtt gcggcgttat ggtatattat ttatttatgc
     7921 aagtccattt tgaatagagg gaagaatata gcgtcaacgt gaagctgatc gacaatcagc
     7981 ataaaatgtt tctcggtatt atgaacgact gttatcgttc ggtttcggaa ggcaaagaaa
     8041 ataaagtttt ggacgacatt ttcgaggagc ttaccaaata ttcccagttt cactttcaaa
     8101 ccgaagagcg gtacttcgat gaatttcatt acgagggggc ggcggaacat aaggaggcgc
     8161 atcggatatt atggcgcgac atcgaggcgt taaaggaaaa aaatcgcggc gacgacgaag
     8221 ccaagctttt gttctaatag gttgattttt tagaggattg acttatccat catatttacg
     8281 gcatggacaa aaaatatact gcatgtttca acgagcatgg attgcggtag tattttcttt
     8341 tggtttgccg ctgttaataa aaataagttc gtgttttgaa gcgactcttt gttatggcaa
     8401 aggcgcgcgc attccctata gattttttaa aatagcaaaa agcgttacaa taaatacaac
     8461 cgacgtcggt taagcgccgg gcactatcat ggatatttac aaagaacagg caaaaaaatt
     8521 ccttcaaagc aattttttgg cggaaatcgc cacggttact ccgggcggcg aaccgcaggt
     8581 cgcgaccatt ttttacgtga tggacgatga tttcaatttc tatttcgtca cgcgccgctc
     8641 ttctaggaaa tatgccaacc ttgcgaataa taacaaggtc ggcatggttg tcacttccga
     8701 aaagggacct gaaaccgtcc agattcaggg cgaggtggat tcgtcgttta aggatattta
     8761 taaatttatt gccatactcg ggacaaaggt tgatttgttc aatttatatt acggtccgtt
     8821 tttgaaactt accggtctgg atttccaggt tttcaaggtg acggttcatt ggctccgtct
     8881 tttaaacctg caagaaggag cggatgtgta tcatcaaatt attcccgatt ttcgcctggt
     8941 ggaagaaaat aaagaaaata agaaggaata gccatcattg atggcgatcc ggctgtttgt
     9001 gcgccgttga tgtcgcgcgg cgtcatcctt cttcaaattt tgtagataaa cattatcaat
     9061 atcaatatat tccatggcgg aaaacgaagc accaaacgaa aaatctctca tatcggcaag
     9121 cgactatttc actttgatgg aaaatacgcc gcttactctt gtggtcttcg acaaggacgg
     9181 taagctgatt ttcataaata aaggcggccg cgaggaatat ttcatcaaag ataccgacga
     9241 catcagcaaa tggagctggc ttgcaacggt caaagagccg taccgcgaca tcgtgagggg
     9301 aaaatttcaa aatgcgctgc gcgggcaggc tgtcaatttt gagttcgaac acacgcccga
     9361 aggatcgaaa tacgcatggt gttcgggttt tatgtctccg atcaaagacg agcatggcaa
     9421 tgcgaaaatg atattttttt actctaccga tatttctcct ttgaagcgcg ccgaactgga
     9481 agcgaaggaa gagagggata tgtttaagac gctgcttgac tccactcccc tctgtatcaa
     9541 gtggtttgac aataaaggag gccttatttc cataaataaa ggcggccgcg aggagcattt
     9601 tctggaaaat ttaagcgagg atcagatcaa acactgggat tacatgtcgt gcatcgacga
     9661 agcgtatcgc cccgaagtca aggaagaaat ggatcttgcg ctcaaaggag cgggcagcac
     9721 ttttgaaata aagcatatcc ccggaacgtc aaaaggcacc tactgcgaat ctacgatggc
     9781 gcccgtgaag gattctaacg gcaatgtgaa atacatctta ttcttatcga gagacatcac
     9841 cgatcagaaa ttgatagggg aagaacgcaa gaaatcagag gaagaattga agaaaaacaa
     9901 cgaggaactg gaaatattca ataagatagc gatcggcagg gaattgaaaa tgatagaact
     9961 aaaagagaaa ataaaggagc tggaagaaaa aataaagccg taatgcattg cggaggcgaa
    10021 gatgacgttg gctggcgcgg gcagggcgcg caacgagctt cctgccgcat tttaaaaata
    10081 tggcggaaaa cgaagcacca aacgaaaaat ctctcatatc ggcaagcgac tatttcactt
    10141 tgatggaaaa tacgccgctt actcttgtgg tcttcgacaa ggacggtaag ctgattttca
    10201 taaataaagg cggccgcgag gaatatttca tcaaagatac cgacgacatc agcaaatgga
    10261 gctggcttgc aacggtcaaa gagccgtacc gcgacatcgt gaggggaaaa tttcaaaatg
    10321 cgctgcgcgg gcaggctgtc aattttgagt tcgaacacac gcccgaagga tcgaaatacg
    10381 catggtgttc gggttttatg tctccgatca aagacggcac cggtgccatg aaagcgatat
    10441 ttttttactc atatgacatc actgctatga agcgcgccga actggaagcg aaggaaaata
    10501 aaatggcgat gctcaatctg cttgaggatg cgcgcggctt cgaggaagaa ttggtaaaat
    10561 caaaaacgaa catagaggct caggtatggg aaaggacgca gcagctgaaa gaagaagaat
    10621 cgcggctcat ggcttccatc aacagtttgt cgctgggatt catcatggtc gacgttgcgg
    10681 gcaaggccat catcaccaat aaggcgattg agcgattttt tacgccggat ttggacgagt
    10741 ccatatttga cgccaccatc cggagtttgc ggggaagttt cgatctggat gcaaattata
    10801 aaaagatttt gagcgaaaag aagcagattg acgcaaagag cattcctttc ggcagcaaat
    10861 atctgagaat attcatggag ccgatcttta ttatcgacga cccggcattg atcggtgttg
    10921 ttgttttgat agaagacatc actgagcagg aacttttgga tcagtccaag agcaattttc
    10981 ttgccatcgc ttcccatgaa atgcgcacgc ctttatccat tatacggggc gacgccgagt
    11041 tgttgcttgg gtcagttgcg ccgtcagacg ataataaaaa tatcatcaaa tacacaaccg
    11101 gtatccgtca gaacagcatg cgacttctcg atattctgaa cgacttcatt gaagttatac
    11161 gcatggatga aaaacctctt gaactcaaga aagaaaatgt caatattgcg gatctggtca
    11221 atgatattat tgcgagtttc ggcaaggctg catcggaaaa aaacataacg cttgtttttc
    11281 aagcgtctgt tgtaccgctc cctgcagtgg tcgccgatct tagtaaggta agggacgtca
    11341 tcacaaacct tatcagcaat gctattcatt acaccgaaaa aggaggtatt accgtcgccg
    11401 tgaatccggt cgtgcgtgaa ttatcgccat ctctggtttt ttctgaaaaa atttcagagg
    11461 gtaaacattt cttgaaaatt tcggtaactg acacaggtat tggtattccg aaagaaaacc
    11521 aatcgatgtt attccaaaaa ttctcgacgg tctcgaaaac gtttttacat acgaaagaat
    11581 acggtagcgg tctcggtctt tatatcgcaa aaatattgtc cgaggcaatg ggcgggttta
    11641 tatggctcga aaaaagcgag cccggcgaag gaagcacatt tgccgtggca ttgccggcgt
    11701 cgtcatgact ggttcgcggt ttattgaatg ccgtggcccg atgtggtttt ttaaataaaa
    11761 ggaatcaaat ttatatttag aaacatggac aaaaaaacac ttattctttt gaccgatgat
    11821 gaatcggaaa tattggatat ttacggttcg gcgcttacaa atgcgggctt tgaggtggtt
    11881 acggcgtcta atggcatcga atgccttgaa gtggcggcag cgaaaaaacc ggatcttatc
    11941 ttactagacc tcaagatgcc ggttatggac ggatctgagg tgattatgaa actgaagctc
    12001 aatccggaaa ccaaagatat taaagtcgtt ttcctcactg cattcagtga tccaatgtcg
    12061 gttggcgttg atgaaagggc cgctaaagaa gcgggagccg ccggttttat aaagaaagga
    12121 ttgggactta atgagtttgt cgaaaaagcg aaaggatatc ttcagggata agaattttta
    12181 gtttctctcg gcattagtcg aggatatgtt ttttgtcgtc ctcgcaaaga tgagcccgtc
    12241 cgattttttc cgaaaaattt tagcgggtga gggcatatgc ttccaatatt ctctttttat
    12301 tttcaaagca tgggtcgtcc gccggagctt gtcctcggcg gagtttatcc cgtactttga
    12361 tatgggaaca ctgggc
//
LOCUS       DAIDDT010000024        11500 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_24_length_11500_cov_5.543290, whole genome shotgun sequence.
ACCESSION   DAIDDT010000024 DAIDDT010000000
VERSION     DAIDDT010000024.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11500)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11500)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11500
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24_length_11500_cov_5.543290"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2925 a         2894 c         2870 g         2811 t
ORIGIN      
        1 tgcggtgctg gtcgttatct gatggccgtc cgtcaggcgt ccccattggt agagccatcc
       61 ataggcggag gaatcggtga cggattgtgc gatgttggcg gtgccgagat tactggcgag
      121 ccagcaattg ccgtcctcgc caatgacggt atggtagatg gtggtgtcgg gagcaaggcc
      181 ggtgacggta ctttcgccgc agacaaagat cggcgatccc gcgcctacgg taaagggaga
      241 ttgcgcttgg aatttctgcg attccgcgtt ggcgaagacc tcccaattgg tgccgccggt
      301 cacataggta taatagaggt tcgaggagga ttgattcacg ggatccacgg gaagggaacc
      361 cagcagggag ccgaacgatg ccttcgtgaa attgacagga atccagccgg tgccgtcgac
      421 atgcgtatag gtggaagatg ccgcgcaatg aaaggatcct cccgagggga agcccagtcc
      481 cgaacagtcg gtgccggcgg tcgtcgtcgc cgtcggatcg ggaatggaga gataggtgac
      541 ggacgaagag ccgagggaga atcctatgaa tcctcctgtg tccgcgacat aggcattgag
      601 ggcggtcgtg agggcgctca gatcggaaag acggttgctg tccctgcttt gtttcaggag
      661 ttcggaggga ttcaggacca taacgaccgc caccatgagg acggcgatga tggcgatcac
      721 gacgagaagc tctatgaggg tgaaggcgga acgggatgct ttgtttgttg ttcttttcgt
      781 cttcatggga gggagaagaa aggaaatgaa agaagcgggg ttactctctc tcgtggggga
      841 ggcttacttt tttgatgcgg tgtctatagt catgatgggt tgtttgcgac gactgaactt
      901 attatattac aacttattta aagatatgtg ccttaattat tattttatgg cttgatctat
      961 tctattcaat cattcttcct ccgttatgcg gattacttct tctatggaaa ccaatccttc
     1021 aagcgccttt atgacgccgt cctggcgcaa cgtgagcata ccctggtttt tcgcttcttt
     1081 cgttattttt tgcgcggtcg gaccggcatt gatgacgtct tcaagcgcgg gcgtcatatg
     1141 gaaaacttcg aagatcgctt cccttccaag aatacccctt cctttgcaga tttcgcatcc
     1201 cttcgcgcgc gtgatcttgt acggttccgg atattttgcg cggacatctt ccggaagttc
     1261 cgcgagcgtc cttcggatga cgtcctgcat attctgcggc gccggttcag ccgccttgca
     1321 gttgtaacac agaagtccga cgagccgctg ggatatcata aggttcaaag aaaccggcag
     1381 aaggaacggc tccactttca tgtcgataag gcgcgggatg acgccggcgg cattgttggt
     1441 gtgcagcgtc gaaaggacga cgtgccccgt aagcgccgcc tgcacggcaa gtccggcggt
     1501 ctccgcatcg cggatctcgc cgaccatgac gacatccgga tcctgccgca atatttcgcg
     1561 caatcccgcc gcgaagtcgt agccgatctc cggcctcacc tgcgactggt tgacgcccgc
     1621 gacaaaatat tcgacggggt cttccaagct cacgatattc ctgctctcat tgttcaatgt
     1681 ctggagaaaa gcgtacagcg tcgtcgtctt gccggaaccc gtcggtcccg ttataagaat
     1741 catgccgtac ggcttcgcaa gcccttcttt cacaatctca agattccggt aaaggaactg
     1801 gaggttgtcg aaactcttca accccgtcga cggatcaagc acgcggaggg cgactttttc
     1861 gccgagcggc gtcggaaagg tcgccacgcg gaaatcgatg tctttattca atatttttgc
     1921 gcggaaccgg ccgtcctgcg gcactctcgt ttcgtcgatt tttaaacttg aaatgacttt
     1981 aatgcgcgag acaacgggct gggcgagttc gatgggaagc gatgcaacct gtttcagttc
     2041 gccatcgatc ctgaaacgga cacgcaatgt tttttcctgc ggttcgatgt ggatatcgga
     2101 cgcgcggccc tgcaccgctt cacggagcgt atcggcgacg attttgacca ccggcgcatc
     2161 ttccgcgctt gacgcgcttt caaggttcgc cactttacgg ccgccttcgc cttcttttat
     2221 attcaatgac tgcactgctt tcgcaatctc gctgcggtag ggggaatacc gcctcaaaac
     2281 atcctgccag tcgccgtaag aaatgagata gacgcccaaa ttgtaacgat tctgccgcgc
     2341 aatgaacttc accgcatcct gcgctttcgg atcctccgga tgcagcatgc cgacgatcaa
     2401 agcgttgttc tcgagagaaa gcggaattgt cgcataagtc cgcgccgttt cctccggcac
     2461 cgtgacaaaa agtttttcat cgatgctttg cggatcgatc ttcttataag gaacccttaa
     2521 aacggcgctt tttatttccg cgaccttcgc atcatccaaa agccgctgct tggctatggt
     2581 cgcctcgagg ggctctccgc tgatgagcgt atctctcttc aggcggttcg ccgccggcgc
     2641 gtcaagcagt ccctgctttt ttatctgctc caaaagttca tcgtcggtca tgatatgatg
     2701 ttctaatgtt gtaataaaaa aataaaacgc ttcattcatc cgacgcaatt cttattgcgt
     2761 cgtccgcccg aaggagggag tccatacttc cgatcttttt tcttaatttt tcaaaatgtg
     2821 aatcgtccag ccaaaccgga cgatgacagg aggaaacttt cttattattt tttttcaaat
     2881 gcgtaagtcc tattttattg cggaggaatg aacggattac tgcggccggt cgaggacgga
     2941 accgtcgaag acggaacata ttgcttcaaa gattggaaaa gcgggttctg tgtcaccgcc
     3001 gatgcatgga tcgtgattcc ggcaagagaa gtggtcgatg cgaaattcgc ggaaggcgca
     3061 acgataaccg attcgggtgc cgcgaaaaaa agataataaa ccgccccgac cccgactgcg
     3121 ataaacacaa gccatcctat aacgctcata atgctcccgc tgctgttttt ttcttcttca
     3181 atgacaatag ccatggtgtt atattttagt gttttaatgt tgtaattgtt ttaacgaaaa
     3241 gctcagcgct ctatcgccat aacattacaa catgagaact ttttaaggca tttggtaata
     3301 tgcgacgatg gtcagctcgt aattgaaatt atcctggttg ttcgccgcgt tttgcggtgc
     3361 ggactgggcg ggctgaatgg aaatattttt catgtcgaaa atcctgatgt tcgtctccag
     3421 ttccgaaaga aaattcttga aatcctcgta actgccgttc gcctcaatgg cgaaagaaat
     3481 gctgtcgaga ggcttcacga ttgacggcgc ggaaacgtcc atttctgtag tggaggacgc
     3541 tataacgggc gcaagcgcaa tattcatggt cagtatgttt atgttattag ttccggcaat
     3601 gccgtatatc tggttaaggg cgccggaaat atcctcttcg gatggaagcg cgagcgcgac
     3661 cgttcccgcc gtctggcttt cgctcttata actcgcaacc aattgctgca cctgctgcgt
     3721 cgtcgaagct tcctgtaaca aaatattctg ctcgctcgcc gcttttcccc ttaatgcctg
     3781 catgtcgcca tacgccggcg acacgacgtc gaaataaaca atgaaggccg caataaggaa
     3841 ggtgagggaa accagcatgc ctacgagtct tttggtggtt tgtttcatga aaaaattaag
     3901 ttcgtttttc cgtaacgcta tgcgttgtta ttatcggaaa gaatcgaagc gttcataaaa
     3961 agagtgacat tcgccgcctg ccatgaacca ttatttactt tcatgggagc gatgccggta
     4021 acggtgatac gcgacacttc cggcgacgat tcaaaaatgg aaatctgctg gttaagatcg
     4081 tcctgagtct tcgcgaccac agtgatcgtc gcctggcccg tggaagaaaa aacgatctgg
     4141 gaataatacg cattcgcctc cgtgttgctt tcgagccacg aaaggaatgg cgtgaaaaca
     4201 acatggttct tcagcaatac ctgcaaatgg gaaagttggg aataaaaatc aatcagggcg
     4261 gtctgatctg cggcaggaac cgtcgtgctg gcggaattta tttttgtttc aaggccggtt
     4321 atttgtgaat tcaaataagg ttcatatcct gcgacaattc cgaaataaat aagaatcata
     4381 aggaaaaagg cccccgcgga aaagaaaagc agacccgaag accatcccgg ggtctgggac
     4441 tcgtcatggc ttaagcggtc gataacttgt tgcggaagtg ccatagaatt atattttggt
     4501 gttccggcgt cgtaattgcc gcaatgaaaa aatgaacact gcaatattat aacattataa
     4561 tctttttatg cgaactgctt cattccgagg ccgagcgcaa cgctcataac tggattcaat
     4621 tccgggatca gcggctcgat agccgggtca tgctcaaagc ccgcgaacga atttgccctt
     4681 acgacaggaa gcgcgaattc cctttcaaag tacggcccta tccccaaaag gttcgcccct
     4741 ccgcccgtta tgatcatccg ttccgttttt tttgcggaag gaaactgcgc tttataaccg
     4801 aactgcgcct tcttcacttc atttattata gcatcaagga acggaatcat gattgtggat
     4861 aactcgcggt tcgggccagt acctaaaata ccccgttctt ttttgagctc ttccgcccgc
     4921 aaagcgttga tgccgaggct ctccgcaaga ttctgcgtga gcgttgccgc cgcaaagtcg
     4981 ctttgtacgt tccaggcaag ccggccattc tcaagaaaaa tgatattcgt ggaacggctt
     5041 ccgatatcca caacgatggt cgacgtcgga tcgccgccga aaatcctcgc caggctcaga
     5101 ctctcgatct cgagcgcccg aagcgaaaga cccgccgccg cgaacatcct ctgatatttt
     5161 ttgatctgct ccaaaggaac ggaaatgaga aggattttct gacgcatgaa cccctcgtca
     5221 tcctgggact ccccgacttt cagccagtcg agcgcgactt cggaaagcgg gatcggaaca
     5281 tactgctttg cctgatactg tatcgccttt tccatttcct gctggctcat attcggaaaa
     5341 tcaagcacgg tcatgaaagc ggaaaacggg gcaatcgacg cgacggcgtc tttcgtcgcc
     5401 gtccccatct cgcggatgac ggtttttaag agttccgtca cgtcggattc gaatatcttc
     5461 aaattactcg tttgcaatgc ctggtttgcg cgggccaaat aaccgctcga ttcaaggagc
     5521 ccgtaattca cgacaaaagg ccgcttgtcg ccctgtttca cttccaccac cttgatcgat
     5581 gtcgttccga tatcaacgcc aagagaggaa ggaaaaacag ttcttttaaa aagattaaaa
     5641 gggttttcca tgatcttgat atttcttcta ttatcatact atgaaattcg atttttttat
     5701 agatatgctg tttccgcggc gatgcctccg ctgccgcgac gcggtaaaaa acggcgttct
     5761 ctgtcccctc tgccgcgcag gcatcgcggt gcacgcatca tttttttgcg gacaatgcaa
     5821 agcgcggata ccaacagaaa ccggtcacga aacggaccct cagactggtt caggccgcat
     5881 ttcaaaaata tgccacaaag attttcccta tgttctcggc gccgcggcat cgtacggcga
     5941 tgaaaccatg cggacgctca tccatcatct caaattccgc ggcgtccgga gcgcggcgga
     6001 accgcttgcg gcgatcatgg cggaatatct cttgaaaacg gggatagatt gcgcgggtta
     6061 ttgcattatt cccgttcctc tttccgcaaa acgggaacgg gaacgcgggt tcaaccagtc
     6121 ggaactcatc ggaaaaatac ttgccgggca tctcgccatt ccgaattttt cacgggggaa
     6181 atcaggcggg catagcgcgt cactcgaaac aaaaatactt gtccgcatcc gccatacccc
     6241 gccgcaaagt tcaacggaaa acgcccgtga gcgcgagatg aacgtccgcg gctgctttgc
     6301 ggcgccaccc gattccttgc gcgggaaaaa tatcatcctt atcgatgacg taacgacttc
     6361 cggcgcaacc ttttttgaag ccgcgcgtgc cgtaaaaaac gccggcgcga aaaaaataat
     6421 cgcgcttgcg gcggccatgg cataggaaag aagaatatag aaaagagaat atagaaatcg
     6481 gatgttaaaa ttccccattc tatgcttctt ttggtatttc ccaaaaaaat gtgatacaat
     6541 aaaaccaatg gaagaagaat tcaaagttaa atggcatgcg ccggaattcg agcaccgcga
     6601 acggaatgtt tcgtggtact ggatatccat gatcgttgcc gcggccatca tcgcgttcgc
     6661 cgtctggcaa aaaaacttcc ttttcggcct ctttatcgtc atcgccgaaa tgctcgtcat
     6721 cgtgtgggga aaccggacgc cgcgcataat cccgttcctt ctcaccgata aaggcgtgcg
     6781 gatcgaaccg gggaagctcc atctgttctc cgaaatggaa agtttcagcg tcgacgaagc
     6841 gctgcatgac gaggaatggg ataccgtcat cttccatttc cacggcacgt tcaagctgcc
     6901 gctcatcata aaactgccga aagaaaagcg cgaggaagtg agaacgcacg tcaagaaaac
     6961 gctcaaggaa accgattacg aaccgtcttt cctcgactcg cttgaaaaaa taatcggatt
     7021 ctaatagtgt tgaagatgtt atagtgttgg gtcggtcggg gcgatcttgt tgtaatgcgc
     7081 aagatcatta caacactgca ttcttatcgc ttcacatgcc ctcgtcgttc aacggatagg
     7141 acgcaagatt gcggatcttg taattgaggt tcgattcctc acgagggcac caataaataa
     7201 taaagctgat ttttagaaaa attttgacgc gtcgcacgaa gcgcggctga atttttctga
     7261 aaaaaacttt gtcctgtcct ggcgcgtctt cattatgaaa ctcacgccgt agtaaaaggc
     7321 ggatctatga atatggtata atttctttag aaaggtcgac tttgttaaat tattaaattt
     7381 caccacttta ttattatgca tcacaaattt cgtaattttg ccataatctc cggtgctgtg
     7441 ctcctcatat cagtatcggt cgcttttgcc caaggaagac cggtttctgc cggcgcggga
     7501 atcaatttgg gaacggagaa ctcataccgc ccatccggtc catacgcatc aatcagctcg
     7561 acctcgacgc aaccttccga acaagattcc gcgcggattc ccgtacgggg acatgccgga
     7621 gcaggaaatc gtcccgccat ggttggttcg acgacaatgg gaggcgtgcg tgcaaccgga
     7681 cagccgtatg ccacacaggg acaagcgcgc ggggcagaga tgcgtcagat ggcgcaggaa
     7741 cgcatcgccg caattcagga caaagtaaaa caacggatcg cacaaaatct ttccggtatt
     7801 tttgatcgcc tcaatcaggt atggacgcag catttctcgc agttgcttga ccgctatgcc
     7861 gccatcttgc agaagataca agaccgtgca aatgcggcgg cggcgaacgg caaggacgtg
     7921 acagcaacga ataccgctat tcaagcggca caggcggcca ttgcgagcgc acgaacggca
     7981 gtagcggcac aggcggccaa gacctacgtg cccaatctct cctccgctac gacaacggtt
     8041 accgccgcca cttcgacacc ccaaggccag ggacagatta tacaggggct gaaagacgct
     8101 ttccagacgc ttcgcacgca actctttagc gacctttttg cgttgcgcga cggcccgatg
     8161 accgatacac gtaaagcggt acagagcgcc ttgcagacgc tcggcaaaat ccccggagtc
     8221 gatcagggaa acgccacttc aaccgaagca acctcgacga accaatagca gataattgaa
     8281 actactatgg attaccaaac aacgaagcaa ggatcctcga agacgttgtg gtatgtcggc
     8341 ggtgtagtcg ttatagtcat cgtcgcactc gccgtctggt atttctcctc gacacagacg
     8401 ccggccacca ccagtacgca aatcactacg agtacgcagg tctcggcaat acaacaaagc
     8461 cagatgccgc cactttccag cggcaataca accgccgaca tccagaatga tctcaaccag
     8521 acctcgaatg gttccgccgc gctcaaccag gatcagaatt ccgtcaacac gagcattcag
     8581 ggtctttaga acccgttcat aatctcatgc cgaagcaaaa ttcatgaatt tcaggcgaaa
     8641 gccgcgctgg gcgagaagag gcgtatcggg atacgccgac gaaccgtaag cggtttgcag
     8701 cataaattca tgagtttttg ccggcacgca ttgattttat tgatattatg cggtgagatt
     8761 atgaacaggt tctcagtcct cggccatgcg gccttgagct tcattatttt ttagctcaaa
     8821 gtaaaaccta gtgaaaaaaa agaacaagtc ctgtccccac gaaggcagag aggattgatg
     8881 aatacccgcc ccaatgggcg ggtattcatc ttaaaatgaa ataaaacaaa aatagataaa
     8941 gaataatgga cggctgttac ggaaataccg acgcacatca agcccatcac cgccaattgt
     9001 aatctctcac gcccctcttt tccgtcgtca ttgaccctca atcaggtcga gggcaggctc
     9061 cggtcgaaga tccgcgcttt ttctgtcgtc ctcgcgaagg cgaggatcca tgctttaaga
     9121 atttaagacc gggagcatgg atcccctcat tcgggggggc gacggagggg aaaatgtttc
     9181 gacggaatcg gcgctggttt atagatagcg aggggttagc gcccattccc ttctcaatgc
     9241 aatggttttt tgttaaagta ggttcatatg aacccgcatc atcgcgccgt acaacaattg
     9301 aaatattgga cggcaaaatt tttcattacc ctgtttgcga ccgtcggagc cataacgata
     9361 atcattttgt tcatatgcac aacacagctg ttgttcataa caccggttac agcgccgtca
     9421 aaatcctcgc caacggcttc atcaactccc gtaaaaaatg cagcttcttc ttcgtcttcg
     9481 cttaaaacat tttcatccaa accggctctg agcagtacaa ctctttccgc gtccaaaacg
     9541 gtttcatcag gatcggctcc cgccggattc cggggtccga caagcccacc gcgcgttata
     9601 ggcccatcgg gcccaccgcc tgactattga gaattacgaa aagaaaagat agaatacaga
     9661 atgtagaaat agctccgtga gcgttgcggg tatcgtatag cggctattat gcgaccttgc
     9721 caaggtcgag atacgggttc gactcccgtt acccgctcca gttttagaca aagttcattt
     9781 tcagaaaatt taaccgcgct tcgcggagcg aagcgtaggg tttggcggga tagaaacgag
     9841 aagacggttg ttaagaaaga ggttcgatcc ggaattttta aagaatatga cccgacagaa
     9901 atttaagccg cctttttctg cgtaagctac catatattct tcgttgacga gaatggcata
     9961 gatctcttct atcgctacgt ccgcattccc atcgagaata attttcctat atttatattc
    10021 tctacatacg aaaaagctcc gacgttatcg gagctttttc ggtatatcca aattttgaat
    10081 gctcaacgtt tagcccactg gggagccata gatatataat aatagagtgc cttagaggac
    10141 cgttctatgc ctctgtctcc cgcaacgaaa tctatcacac aagaatcgta cgaaatatta
    10201 cgcaacagca ttattcaact gataaggtta accaacaaac cttaaaacga agctgtttag
    10261 aacttattca taatctcatg ccgaagccaa attcatgaat ttcaggagaa aaccgcgctg
    10321 ggcgagaagg tgtatcggga tacgccgacg aaccgtaagc ggtttgcagc cgaaattcat
    10381 gaatttttgc cggcacgcat tgattttatt ggatattatg cggtgagatt atgaacgggt
    10441 tctaaaatca gaattaatga ttctggaaat atgctctcgt tatcgggccg aagtaaccgc
    10501 tcgccgggct tatgcctact ttcgcctgaa actctgccaa tgcactccgc gtaaggagcc
    10561 agaaatatcc tgtagcaccg gttacggcaa gcttttgagc ataacgtccg ctgttttgat
    10621 tgatgagaaa acgctgtaat gacgcgacgt cattgccccg cgaacccatg gtcaaatttc
    10681 ggaaaaacgt atttgatgaa cctgactgaa taccggcagg gaacgatatc cccctttggg
    10741 aagcctcttt ttcaagggtt tgcaatgtcg caagcagaga attaagttgg gcttgcaatg
    10801 attgtattga aggagccgca ctcaccgtag tagtcgccgt caccgtagaa acgccagtca
    10861 aagtaatagt gtgttgagct tgatacgaat tacctccggc agtgaaaaac tcaccatata
    10921 ttgtataaga gccgggagag cacgacgcta cgccgcaaag attccactgg agcgtcgaag
    10981 cataggggat tggagaaaca gatgcaaaat tacttgaaga ggttgcaaat gccatcgtgg
    11041 aaatattgac ggccgcgggt atttccaatt cgacggtttg attcgtcgta gtaagcgccc
    11101 cattattgat catgaaatct ggtaatggag ctggcaacgg agctgcgcca ccgccgctgg
    11161 cgacggcaac gacggtggaa gcggcgcatg tatatccctg aggcgtaaac gtgtagattt
    11221 ccgaataggt cgattgagtg atggttgcag tctgaatacc acttgaacaa cttaatgcca
    11281 ctgatccccc gctcccgttc gtggatatcc cgagcgactg aggagaggta agcgtaaagg
    11341 tgccccccgt cgaactcgac atcgtaacga cgaccgtccc cgaattgacc gtaagtaagt
    11401 ctgcgacaga tccagcagaa accgtcaaat tcattacggg ggaagttaag gataggatag
    11461 tgtttgtact ataggtaagc ggcgcagcat acgtttcttg
//
LOCUS       DAIDDT010000025        10811 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_25_length_10811_cov_6.417365, whole genome shotgun sequence.
ACCESSION   DAIDDT010000025 DAIDDT010000000
VERSION     DAIDDT010000025.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10811)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10811)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10811
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25_length_10811_cov_6.417365"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2887 a         2904 c         2670 g         2350 t
ORIGIN      
        1 aaacaattaa ttaaataaga acccgtctca aaagtaagcc agcgggaatg aaacaaagtt
       61 tcatagaatt tccgcacaac ccgcggatcc tgcctgaaaa gctgcttttg agacgggttc
      121 tctgtttata tgttggtatg gttaattagg tttatatgcc gaccttgcat ttattactac
      181 acgacagcaa atatcttttt cgggctcggg gtactggagg aaaagtcgaa tgaaaactcc
      241 ggtgtcccag cccgctatta cttactacac ataagaaccc gtctcaaaag cagtttttga
      301 gacgggttct ctacattttt ttcggaaacc cgttcacgga tccttaccgg ccgatgaacc
      361 gtatgccgcc cgcggtgatg gtgccgctgg acgatcgctc cccgaatatc acgacatcgt
      421 caccgacgcc ggaaacgccg cccgatgaaa aatatacgcg aggaccggcg ataaccgtgg
      481 atgtctggcc gccgtcatcc tgaatgacaa acccgtttgt cgttatctct ataatcatgc
      541 cgcgatgaat atcatcgaaa cgctccatgc cgaatccgcg gtaaaattcg ccgaggatag
      601 gcaagtcgtc gtgccgggcg gaatagaaaa gcgacttatg aaaagatgtc cgggtgatca
      661 ggaatccgcc caccacggcg accgcgacga tgcccgtaaa aagatatact gccggccgtt
      721 tataaacgaa agaatatcgt tttacaagca cggcaagaac aatcataaaa acagcaagaa
      781 gaaaaacgag cgcccacggg agcgatcgga gaatcgaata ccatccggca gcaccaaagt
      841 ccggcgcgaa ccataatccg gtttcctgga gcataaacat aatgaaactt gccagataaa
      901 gcagcataag cagcataata atgccgccca cggcaaaaag aacggcttgc agcacaaaat
      961 gccaccgcgg ctgcattttc gctttgccgc tccggatggc ttcgagcatc ttctgctccg
     1021 cctgttttgc ttcgccttga tttgaatgag aatccataat tttatatctt ataggacata
     1081 cgcacttgtg ccgggtacag ggcattttct tatgtttttt ttcaaatgcg aaagtcctat
     1141 attatagtac actacggttt gattattttc ccgagcatca gcttcccccg cttgatcctg
     1201 gttgcgatgg tcgaaacggg aacctgaaga atttgggaaa tcgttttgta atcaaggtct
     1261 tcgaaataat aaaggacgag cggctcgcgg tatttcgggt cgagcgatcc gagcggcgcg
     1321 tcaagcatcc gcctcatctc ttcgcgcttc gcatggtcat cggttttttc gtcggcgacg
     1381 ggatgcggaa acaggacgtc ggcatcaaag agcgagaaat tcgcccgatc cttcgcgctg
     1441 tcacgcaggg cgttcacgaa aatattatgc gcaatgcgat agatccatgg cgaaaaacgg
     1501 cgcgacggat cgaatccctg aatattcgta tacgccttta tgaaaacatc ctgcacgaga
     1561 tcggcaacgt catcgccgtg gaacaaaaat ttccgcgcgt atcgcgccaa tttcggctgg
     1621 tagcgaagca ccaaaacacc gaaggcctcg cggcctcctt tctgcacttc tctgcacttc
     1681 gcgcgcaata tcttcatcgg tctgttccac tgcttaataa tacggttttc ggggcattaa
     1741 agtttcaaaa aaaagaaatg cgtattgccg cggataaaat gaataacgaa ttatgcgccc
     1801 ttcacttcac ggtccggatg ttttttctcg atacgtccgt gcggcttcat gaaatggctt
     1861 gcgcttacgg cgatgaaata cacgaccgca gcgatggcgg cgatataaaa actcacggga
     1921 tatctgctta tgagcgcgag aaaaagtccg ccccacgtga ataaaagtcc gagaaggatc
     1981 gctatgccga tcgctctgcc cggcgtccgc gcgagatgct gcgctatcgc cgcgggagcg
     2041 atgatcaatg cgaataccaa taacacgccg accacctgca cggccgaggc gattgtgatt
     2101 gcgagcaata acatgaaaat tatcgaaagc atattgaccg gcacgccgcg cgcttcggcg
     2161 acttcggggt cgatcgaagc aaataagagc ggacggaaaa tcacggcaag aacagcgagg
     2221 gtcccgatgc cgaagataac cgtcaaaacg atgtctgcgc gcgttacgct taaaatggaa
     2281 ccgaaaagaa ctcccactgt ctcacttgcg gaactcgtgt acaaccgcag gaaaaggacg
     2341 ccgagaccca gggcgaacgc aaggatcatg ccgatctcga tatcacgccc gcggatgcgc
     2401 ttccccaacg cgcccatgcc gagcgcggcc gcggccgtga atcccgccat accgacaagc
     2461 gagctcacgc ccagcaatgc ggcgcaggtc gctcccgcaa aaccgatatg cgacaacgcc
     2521 tcggtcgcga acgcctgcgc gcgaagcacc acgaaataac caataagcgc ggcggtaatc
     2581 gcaatcagcg ttccggcgat aaaagcgttc tgtatgaatg tttgatgcag aagttctgac
     2641 atataaaaat gtttacgatt tatgaatcag gatatgaaga aacaccgggt tccatttttc
     2701 tttttttgca gattgcaagc attgttttat tccgttttaa ttttaacagg acgtacgcgc
     2761 ttgcgccggg tacgaggcat tttccggtta ttttccggga catagccctg ctacggcgat
     2821 gaaaataatc ggaaaataac gaaggaacgt gccttgtccg ttgcggtttg aaacattttc
     2881 ttatgatttt ttcaaatgcg taagtcctgt cttataactc cttctgtccc aacgcggcga
     2941 aagcaaccgc acgggcaaat ataccgcgaa tgacatggcg gcgatataaa aactgaccgg
     3001 ccagcttccg gttgccgcga gaaagatacc aagccataca taaacaagcc ccagtatgac
     3061 ggagagcgca atgacggtga ccggcgcatg cgcgatccgc gtcgcggtcg ccgcgggacc
     3121 gaccagcaac gcaaaaataa gaattacgcc gactacctgc accgatatgg aaacggcgac
     3181 ggcaaccatg acaagaaaaa taatcgcaag cgggcggacc ggcacgccgc gcgcttcggc
     3241 gacttcgggg tcgatcgaag caaataagag cggacggaaa atcacggcaa gaacagcgat
     3301 aatcgcgatg ccgaaaacag cggtaacgaa aacatcggcc gtgctgatgc cgaggatcgt
     3361 tccaaaaagg atgctgtagg cctgttccgc atagccgtta taaacggaaa taaaaagcgc
     3421 gccaagtccc agcataaatg tcgtcgtgat gccgatattg atatcttctt cccggaaacc
     3481 tttcgcgaaa gtcccgatac ccatccccgc ggcaatcgtg aaaaaaagca ggccgaagag
     3541 cggatcgata ccaaggacaa gcgcgcccgc ggcgccggca aaaccgatat gcgacaacgc
     3601 gtggccggcg aacgtaagcc ctctcgtaat aagaaagtag ccgacgcacc ccgcgacgat
     3661 cgcgacaagc gatccggcga aaagcgcatt ttgcatgaaa ggatattgga aaacggaaag
     3721 catattgaaa aataatgatt ggtttttgtt cttattgttc ttatctccct ctgtcatcgt
     3781 ccgcccttga tcgtagccga gggtaagctc cggcggatga cccatgcttt aaaaaaattg
     3841 aaagcatgga ttcccgcccg ccgaaatttt tcggaaaaaa ccagggcggg gctcgccttc
     3901 gtgagaacaa caacccgaaa aatagttatg tttccgcacc gagcacgaag atgcggccac
     3961 cgacccgcat gacctcaacc ttcgtaccgt aaagatcgga aagcgtttcg ccggtgatga
     4021 tttcctcggg aacgcccatc gcgccgcggc cgttcgccat ataaagcacg tgatcgatgc
     4081 tcggtaataa tggatttacg tcatgagcaa ccagcatgac ggtcacggcg cggcggcggc
     4141 aaatatccgt aaccaaagcg atgatctcct gcgcatgatt gatgtcaaga ttggaaagcg
     4201 gctcgtcaag caataagatt ttcggatccg tgataagcgc ctgcgcgata agcagccgtt
     4261 gctgctcccc tcccgaaagt tttcccacgg gcgcgccgcc gaactgttcc gcatccactt
     4321 cttttaaaac gtcatcaatc gccgcctccc gcttgcggtt tgcgaacccg atgccccatt
     4381 tgttcccgtc cagtccgaag cccacgacgt cgcgcgcgcg caatgcgaga tccgcttcaa
     4441 gaacgcggtg ctgcggagca tacccgatat ccttattgcc cctccgcggc gcggcgccga
     4501 gtaccgaaac gatcccgccg gctggcttca aaagtccaag caataatttt aaaagcgtgg
     4561 ttttccccgt tccgttcgga ccgagcaccg cgacaaacga tccgcgcggc accgcgaaac
     4621 tgacatcgtc caggatcgtc cggtcgccga gccggacggt aacgtctttc aatgagattg
     4681 cgatttcgtt tttgttttcc atgatgacag taaaaccagc gcgcttgttt taaataaaat
     4741 tatagaaccc atctcaaagg caggccagcg gacactgctt gaaaagccgc ttttgagacg
     4801 gattctaatg tccggttgcc tgcgcaaggc cttgttccaa agcgttcagc tcatccatta
     4861 tccatttttg atacgtggcg gaaggcggca tggtctcgga caccggaacg accggaatac
     4921 cgttcgcctt cgcggcatcc tccaggtttg tcgtaatggg cgtcaccgcc tggctgttat
     4981 aaatcaaaac tttcacctgt ttattgccga tctgattatc ggcagcgctc acgtcctgca
     5041 ccgacggatc attcccttcc gcaatcgcct tttcgaaacc gaacggggtc agaaccttta
     5101 accccatcgg ctgcgtctgg tacagataaa tggtttccgt gagcgcgacc ggcgtgccgg
     5161 catacttcgc tttgatcccg ttcattttat cacggatcgg cgaaagagag ccatcgaacg
     5221 ccgcaagatt attttcaaaa acggccgagg cggccggatc gtttttttcg agcgcatcgg
     5281 taatgctccg cgcgatcgcc acaatattgt cgatcccgta ccaaacgtgc ggattatcag
     5341 gcagcggatc cggcgcgata tttcccgcga taataacggt gcggttcgga ttcggcgatg
     5401 cggtaatgag cttgtccatc catgtgtcgt aatgaagccc gttctcgatc acgatatccg
     5461 cgttcgcaat ggcgacggca tcctgcacgt tcgcttcgta ttcgtgcggg tccacgttcg
     5521 ggtcggaaag aatgctcgtg accgtcgcgc gattgcccgc cagctgtccc gcgatattgc
     5581 cgtaaaaatt ttccgcggcg acgatgcgga tgacgccgct tcgggcatag ggctgcgata
     5641 aatttcccga tcgaatggca aaaaaaataa aaaaggcagc tatcgccgtc acggcgatta
     5701 ccataagagc aacggggaaa attttattca tactattgat tcccgatccg atattattta
     5761 cgagaacccg tctcaaaaac aggccggcac gctccgcgga cactgcccaa aaagccgctt
     5821 ttgagacgag ttctccatgt tattctataa tattgccaaa acggaatttc cgcaatttag
     5881 cgggcgtacg cgtccgtcaa cggcaacgtt atggtatttt tccattattg gggtatgctc
     5941 atcaaaacca tcgccatgca atcaggattc tggaaaaaac tgaaaaagcc cgtcatggcg
     6001 attgcgccga tggcgaatgt cacggacgcg gcgttccgac gaatgtttgt tgcgtgcggc
     6061 aaacccgatg tcttctggac agagttcgtt gcggtggaag gactgctctc aagaggaaag
     6121 gaacggctcc ttgtcgatct ttggtacgcc gaagcggaac gtccgatcgt ggcgcaggta
     6181 ttcggcgcga agccggaaca attcgaaaag gcggccttga tcatccgcga actcggcttc
     6241 gacggcatcg atatcaatat gggctgtccg gacaaagacg tcgaaaaaca gggcgcggga
     6301 gcggcattga tgaaaaacac cgcgcgcgcg aaacagatca tccgggcggc aaaacaggga
     6361 gccggaaaaa tgccggtctc cgtaaaaacg cgcatcggtt acgacaggga tcaaatcgac
     6421 gaatggatcc ccgcgcttct cgaagaaaat atcgccgcgc ttaccgtgca tctccgcacg
     6481 agacgggaaa tgtccgccgc cgccgcgcac tgggaactcg cgccgaagat cgcggcactc
     6541 cgcgatcgtt atgcgaaagg cgctatcatc ctcggaaacg gcgacatcaa aacgcccgac
     6601 gaagcgcgcg taaaaataaa agaaacgggc atcgacggcg taatgatcgg ccgaggcgtc
     6661 ttcggaaacc cgtggttttt ctcggaacgg gcgcccggtt gccgtgaacg gcttgcaggc
     6721 atggtcgcgc acgcggaact tttcgaaaaa atatacaaaa gcaaccgcac aaaaaaagac
     6781 ggcggcctca aaaacttcga catcatgaaa aaacacttca aagcgtatac cgccggattc
     6841 aaaggatcga aagaactgcg catcgccctt atgaaaacga aaaacgccga agaggtaaaa
     6901 aagataacgg aaaaattcat ggagaaaaaa tccaaaaaaa aaatttccga tatgtacatc
     6961 cgtgaacctt catgttaaaa tgcagtcatg atcatgaaag tatcaatcgt tattccggcc
     7021 tttaacgagg aaaaaaatat cgctgacggc atccgcgcgg ctttggcgca ggattatcct
     7081 catttcgaag ttatcgtcgt cgataacggc agcacggaca acacggcggc gatcgccgcg
     7141 caatttccga tcacggtaat tgccgagccg cgcaaaggca cgaatcatgc ccgcgaaagt
     7201 ggccgccggg tggccacggg cgaaatcatc gccaatttcg acgccgattg cctcccctct
     7261 cccgactggc tttcggtcgg cgtggcgcgc ctgaacgatc ggaatgtgag cgccgtttcg
     7321 ggaccttacg attattacga cgccgggcgg gtttccagaa taatattact tgcactgcaa
     7381 aaaaacgttt ttacgctggc gaacaaaatc acttctttcc tcaaccgcgg cggaacgatc
     7441 attgccggca atgtccttat ccgcgcttcg gcgctcgatg aggccggcgg ctataacacc
     7501 gcgatcgttt tttacggcga cgataccgat accgcgcagc gggtttccag gcacggtaaa
     7561 ataattttcg accgccggct catcgtgcga tcgtccgcgc gacgctttaa agctgaagga
     7621 actttcaaaa taatttttat atatgttttt tatttcttcc gcataacgct tgcgaacgct
     7681 gcgcataaag aaaaaaggaa ataatgtccg tctcaaaagc agcttttcag gcagcgtccg
     7741 caggttgtgt aagagatatt tgtccgctaa aattttttga agagaaaatt aagcggacgc
     7801 gcctttacgg ggacgacgga gaagtttttc cgacagcacg gcataataca aattctcaag
     7861 ctgcgtgacg ctttcattga tatcgtaacg cttgctgctc tccaggctct gccgacccat
     7921 ggcttccagc gttttttcgt ccgacagcat gacaagcgct tttttcgcgc aatcatgggg
     7981 atcctgcggt ttgaaaagca atccgttccc gtccgcgaaa tatttcgaag cgctgttcgg
     8041 ggcatcggca atgaggacgg gcgcgccgca cgccatggcc tcaagcacga ccatcccttc
     8101 catttccgca agcgacggca tgcagaaaat agtgcaggcg ctgtacgcga gcgccttatc
     8161 ttcctcgctc attattcccg tgaaaatgac cttgtcttca acgcccgcac ccttcgcaca
     8221 ctgttcaatg acgttcttct gggtatcgtc gccgacgagc aacacacgga catcggaacg
     8281 ggttttcaat atttcgggca gggctttcac gagcgtaacg acatcttttt caggatgcgc
     8341 caagcgtccg acgaataaaa tatatttatt ttcgcgcggc aagcggtatt tttcgaaaaa
     8401 cggcgcaggg tcgcgcggcc caaaaaaaga cgcgtcgacg ccgttgctta tcacggcata
     8461 acgcatgcgt gcatccatcc gcggcggctg ctcccgcgca aattcggtcg gataaataag
     8521 cgcgtcggct tttttatata cccacgaaag ataggctatg aaaatcgcgc tcaataatcc
     8581 gcggccgagg atctttggga cgtacaaaaa aacgttttcc gcttgcgcat gggaaccgat
     8641 gacgaccgca attcccattt ccttcgccgc tttcaaaaaa acaaaaccca tcacggacgg
     8701 caggatgatg tgcagcacgt cgatccgttc gtcccgcagc gcctttttcg cttccgcaac
     8761 ggtggcataa gcaaaataaa atttatgctc ggtgttcggc ataagcagcg acctgaaccg
     8821 gtatgtcttc gtgccgtcca cgtccccctg cgcatacggc actttcgccg caatgaaaat
     8881 aatggtatgc ccgcgcgccg cgagccgttc cgaaaaacgc aacgtggaaa ccgtgcttcc
     8941 gccggtcgta taatcaatgg catcgcacac catggcgatg ttaagttttc ttttttgccc
     9001 cgttgttttt ccatcgaaaa atccggcttg acgcgcggcg ccattctctt ttaagtcatc
     9061 gggtgtcatg attaaaaatc ttttatttct ttcttgtctt taaatttttt aacatccgtg
     9121 cgcttgtaaa aaatgatggc aaccacggca agcgccgcca cgcttataat aacggcaaat
     9181 tctttcaaat aatgcgctat ctggacgtac gcgccgccga aaatgatgcc gagtatcagg
     9241 aaaaacgccg actggaccat gctgaccaga aaacacgtcg tgaaaaaccg cttgtacgga
     9301 atcttaagcg atcccgccgc gaccaatccc gtcgtgccga taccgtgaac gatcttcgaa
     9361 aggacaagtg ctttgcggtg attttccttg aaatagtcgt tcgccttgtc tattttttct
     9421 ttcgtaacac cgaaacgatg cccgttgcgc ataagaaatc ctccgcccca ataaccgacg
     9481 ccataataga aagcatcccc taccacatcg ccgaagacaa cgatggcata cactatcaac
     9541 gggttcatta cgcccgtcgt cactaaaaaa ccggcgatga cggtcgtaat agggccttcg
     9601 aaaatcgcga aagggagaag gatgggatac cggtaggcaa gaaggagcgc caagacatta
     9661 ttcatgattg cggtaaaaaa taataaccgc ccgccgcgga cggcgggcat cctcgtcatg
     9721 atagcatatt tttactccat cgtcgcttgc atgttccgat ccctcccttc gcccgaggga
     9781 gacaggaagg gttcacgtct taatttcttc aatttcgctt tcaaagcgcg gatacggcaa
     9841 tgacggaaaa gatccgcgcc ggaattcatg aatttattcc tggctatgat caggaacggc
     9901 aatggcatct gaaaataatt tcctcctgct gtcccgcaaa agccaatgga taaaaagcgc
     9961 ggaacaaatg ccgattacgg cgccggcagc gacatcaaac ggccaatgga cgccgacata
    10021 gatgcgcgcg atgacgatca cgagcgcccc gataaaaaac cacagtccct gtttacggtt
    10081 gcggaaaaaa acgaccgtgg caagcgcgaa aaaccacgcg gcatggcccg acgggaatga
    10141 aggccccgcc gcattgataa gcggcgtaaa acccaggaca tcaaacggcc gcggataatg
    10201 atagaaaaaa tgaatgccct cggtgataag cccgcgggaa agaataatcg ccaaagcggc
    10261 ttcacagaaa agataccact tcccgcgccg gctcttttcg taaaaaacca taacgaaaaa
    10321 aataagcacc aaaaaatacg gcagatcctg cgcgcaaaaa acggcgaaac cgtcaagaaa
    10381 aaaattccgg cggctcagtc cgtaaataaa ttggaaaagc gattgattaa gaaaagacat
    10441 gcgttgtaat gccggatcgt tctaatgttc tcatgccgca aaacatccac aacgccatac
    10501 tgcaaccatc gtaacacgga acatcaaaac aacttcattt ccggatcatg gatattcatc
    10561 gcgggctcga aattccatga tgaccataaa atcagaattt tatcgtcatt ttctcacata
    10621 cggaagaagc gccatgaaac gcgcgcgctt aatggcttcc gcaagcatcc gctcgtgctt
    10681 cgcgcacacg cttgtatgct tcggactgat gattttcgcc tggcttgaaa taaaacgctt
    10741 caggagatcc acctccttat aatccacttc tctgatattc tgcgaacaga aaaaacactg
    10801 ttgtttcata a
//
LOCUS       DAIDDT010000026        10338 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_26_length_10338_cov_5.559205, whole genome shotgun sequence.
ACCESSION   DAIDDT010000026 DAIDDT010000000
VERSION     DAIDDT010000026.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10338)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10338)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10338
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26_length_10338_cov_5.559205"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2676 a         2652 c         2614 g         2396 t
ORIGIN      
        1 agggcgagtc tccgcgggga cgacagaggg gacaagggga caacggaggg gaaaatgttt
       61 cgacggaatc ggcgctgatt atagacagtg agaggttgcc gttttttgca tgttttgaca
      121 ggaatcttcc gacactatcc ggtattactt gcaaacgcat ttgcattttc cgttcgtgca
      181 tttacagacg catgtcctgc atgcgcacac cccgcgcgca caaccgcacg aaacggtccg
      241 gagcgttgtt ttcgatgacg caccggcttt cttttttgcc gcacggaccg ttttggacag
      301 cgtccctatt ttcttttttg tccgggaaac cgtcttggat gatgtcttga ttttcttttt
      361 tgtttttgcc atgtttttta ttttttatcg gtgattgata attttttata gacgaccttt
      421 tgtttccgtt ttgccgccga tcataatctc acataatctc acgcttcccc gaaaagatta
      481 tagtgataca caacgccgag atagagaccg gcaaggataa aaaatccgag aagccccgtg
      541 gcaatcgccc atcttgaacc tatccgcgga gcgagatatg ccacaagaat catataggga
      601 acccacgaaa tgatcgtgcc caccagcacg aaccatgcgt gcgcgccgac cgcttttccc
      661 ccgctcgtct cgccgatgaa ggcataagca acaagcgtga agacgggcat aagcgtcgca
      721 aatcccgaaa ggagacgcgc gccgttctct tcaagcgcga cgatgaggac ggtaaatatg
      781 ccgcccgtca ggaaatatat gaaataactt ttcatccacg gccacatttt tccattatag
      841 cggatcgcgg tacgcaagca aattcatttc ataaaataac ggttcgaaaa aaaatacatt
      901 acatgaatcg ttttttcgcg caaccggcaa acgccggtga tgcaaaaaaa taaaaccccg
      961 cagcggcgcg cggggtttcc aatgcaaagt tatttaccgg gactatcatc cgccgaagcg
     1021 gaacgcgaaa acacgcgttt aattccataa tatatcccgt cgaatgacgc tgcctgcggc
     1081 gtcacttcat atcccccttc gtttcccgtc gcgcacacaa ccataggagg atacgtcgga
     1141 ttgctgctgc ggatccaaag accgtatttg cggctgagcg ccttgagttc ttccgcgaag
     1201 gaaagtacct gctctttcct gctcggcgtc ttccacggca attcgggcca tgcgcattgc
     1261 gccatcctga gaatattttc aatctcgtgg cagcttatag gcataggctt tctgattttt
     1321 gccataagct cattttcggc gaccggcgtg aggcaatacg gcggcgacat tccgaatggc
     1381 gcgccgattt cattttcagt aatcgggccg cgcgcatccc accatgattc aggaccgcgg
     1441 acgacagcgt gcgcgataac atcgccgcgc atcaaaccga ttatcggcca acccgttccg
     1501 cgcgaaagcg cgatcgcaag aatataacat cttccgttga gacatccagc aagaatcact
     1561 tcgcggaaca ggtcccgata ttcttcctct tttccttttc cttccggcat gacaacctcc
     1621 tttttattat taatgaggat atataaggtc ttaagaactg tttacagcat acaaaaaata
     1681 aatcattttg tcaaaaaaat cggaagcggc cggcgcggaa aatgcataaa gcctcataca
     1741 gcggtttgaa ttcgcggaag aaaaatatat actgtcttta tggatcttct tgttttatca
     1801 aaagaaaaaa ccgcgctggt cgtcatcgat ctccagaaag gcattaccgc gatgccaacc
     1861 gagccgcacg atgccgccac agttataaaa aatgccgcgc agcttgcggc atcgttccgg
     1921 tcgaacggca tgccggtatt cctcgtgcgc gtaacaccgt cggcagacat gaaagacgga
     1981 cttcgaccgg ccgccgacga gccttttgca atgcgcggcg caatgcctcc ggactggagc
     2041 gtcattgttc ccgaaatgaa acaacaggaa tccgacttca tcgtcaccaa gcgccaatgg
     2101 ggcgcgttct acggcacgga actcgactta cagctccggc ggcgcggcat cgacaccatt
     2161 gttctttgcg gcatcgcgac cgacatcggc gtggaaagca ccgcgcgctt cgcgtatgag
     2221 tacgggtaca gccagatctt cgcggaagat gccatggcgg gacgttccgc cgaaggacat
     2281 cgccatgccg taaaggaaat ttttccgcgc atcggaaaag tccggacaac cgaagaaatt
     2341 cttgacgcga cgggataaga taaaataaaa gtccgcctga tgaaaaaggc cgcttcctca
     2401 ccgcgcgaac ggttcaaaat aaataaatgc ataatctcga acaaattccc ggcaattccg
     2461 gcgatacggc ggataacgaa aaaaatcctg attttaattt taccgctgcg gatgttttga
     2521 aaaccctccg cactgcatcg gggaaaccga gcattgaata tatcggcagc ggcacatcgc
     2581 attttcaggc cgaacccttg gtatacggcc cggacggcga accgctggtt ctttcggact
     2641 gggagtttga agtcgtaaaa aatctcaaag gaaaagaaaa tgcgaaaata aaaggaaacc
     2701 tggatcccgc cgctttcccg cgtttccttc aggagaaaga aaaatatatc gaccgttccg
     2761 cggaacttgg agagaatatg ttccggtttt cgctcgattt cgcgcggctt tgtccgaagg
     2821 aaggcgagtt tgataccgaa cttatggccg actacgtgaa ggcgctcgcc ttgatccggg
     2881 cgcgcgggca ggaaccgatg cttacgcttt atcactggcc gatgcccatg tttcttgtga
     2941 aaatcaacga aaacgaagat atcacaaagg gaggatggga aaatcccgag gccgcaaaac
     3001 atttccgatt ttacgtcgaa aacgtcataa agtttctggc cgacgaggac aagatccgcg
     3061 gcgtgctggc agaggaaaac ttcgacaaag aatcccagga taaattcctc gccgaaggac
     3121 tcgttaaata tttcctttcg ataaacgaac cggtcaatat agcgggactg aattacatcg
     3181 caggcatatt tccgccttac aaaacaggaa aaatcggcct cgcaaaaaag gtgatgcaaa
     3241 aactgattga tgcccacgat atgacacgcg accggctgaa aagcggcggc atgaaaatga
     3301 cggaaggccg tgagccgcag gtgggcatca gccatgcctg gaattatttt gacggcattc
     3361 tcggcggcgc ttttcataaa attttcaata aaggccctgc ttcaaaattt gaacggaata
     3421 acgagtacac ggattttttg gcgctgcaat attacttccg catgacggcg ccgttgtttg
     3481 ccaaacgcgg gagggactac ggagacaatc ctgctttcgg cgacatatat cctccgggga
     3541 tttacgagat gcttaaaaaa atgaataaag aatatcccca aaaggagata ttcattaccg
     3601 agtttggttt ttccgagaat aaagacatgc ggcgtccgta ttggatattg gaaactgtgc
     3661 gctatatcat tgaagcgaaa aaacacggca tcccgatcaa aggcatgctc ctttggtccc
     3721 tcgtgaacaa ctttgaaatc acgcttccct ctgaccagaa attcggcctc ttcgacgaat
     3781 ccgaacttaa tacgccgctg atacgcggaa cagaagggat aaaaagctgg gaggtctggc
     3841 agtcggctat cggcgcgatc gcaaaccctt cggaagaatc tcttaaaaag cttcaggatt
     3901 gttatgagac ggcgcagcga caatacagaa acgccggcgg aaagtatggg agcccgtccc
     3961 taaaataagg attgtttcgc cgaaacgaag cgagataagg cggaaaaatc gattaggaga
     4021 aaccggaatt tttttgttat cattacgcta tggtcaaaaa aacggagcta taccttcgta
     4081 atgtcatttt cggggttgcg gatagcgtag tttcgaccgt cggccttctt tccggcatcg
     4141 ccatcgccgg cacgccgcaa cgcaccatca tcctcacggg tatcgtatac gctttcgtcg
     4201 aagcgttttc gatggcggtg ggaagcttcc tttccgaaca atccgccgaa gaatacgaag
     4261 cgaaaggaga agttcctgac ggcggcccgc tcgccggaag cgctgcgatg ttcatttctt
     4321 tcgtgctggc atcgtttatt cccattgtcc cctacctcct attcggcccg gcggcagcgt
     4381 tatggctttc cgtcgggttt tcgaccgccg cccttttcat cgtgggagcg ataaacgcga
     4441 aaatcgtaaa agtgaacgcg ataaatcatg gcattaaaat gatgttcctc ggcggcgcgg
     4501 ccatcgttat cggcgtgatc gtcggaaaat tcatgaaagc gggattatga ttttaaagta
     4561 cggtttcccg actgtaaaaa tttttcggaa aaaaaccttg gatagacccg ccttccgttc
     4621 agtaaattca aatcaagagc ggggacgacg gatataaagc aggacttatg catttgaaaa
     4681 aaaatcataa aaaaaattct caaaccgcaa cggcacgagt gcgtacgttc tataaagaaa
     4741 gaactaaaaa tggagacacc tgatttaaaa acgttcgcgg gaaaaagcat caacgatatt
     4801 tttcgaggtc tcgaaacttc aaaagacggc ctgacggctc cggaagcggc gcggcggttc
     4861 aggcaatacg gccccaacga attcgcggcg aaaaaaaaga tacggcccgc gttcattttc
     4921 ttttcaaagt tcaggaatcc gcttcttatt ttattactcg cggcggccgt ggtcaccata
     4981 agcattggct ctgtttttga aggaaccata atcatcgtga ttgttcttgg aagcgcgaca
     5041 atcgatttta tcaacaccta caaatcggcg aaagcggcgg aagcgttgca ggaaaaagtg
     5101 agcatcacgg ccgccgtgat gcgcgcgggc gaaacagagg aacgggccat acggggcatc
     5161 gttccgggcg atgttttcgc gcttacggca ggcgatatcg ttcccgcgga cggaattctt
     5221 tttgaagcga aagatctttt cgtgaacgaa agcactttga cgggcgaatc gatgccggtt
     5281 gaaaaaatcg ttcaggacgg cgcggcgcgg acggttctta tggaaacgag cgtggtttcg
     5341 ggcgaagggc ttgccgtcgc cgtcatgacg ggcgcgcgga cgaaaatcgg aagcatcgcc
     5401 ggaaagctcg atcagccgga tacgccgacg gagttcgaga aaaacctcaa ggatttcagt
     5461 ttgttcattt tccgcacgac gattttcctg gtactgaccg tgatccttct gaatattttc
     5521 tttatgaggc gcgatctgct gcaggtgttc cttttcgccg tcgcgatcgc cgtcggcctt
     5581 acgccggagc ttctgccgct catcgtaacc agtaatcttt caaaaagcgc gctcgcgatg
     5641 gcgaagcgcg gcgtcatcgt gaaaaagctc gccgcgatcc acaatttcgg cagcgtcgat
     5701 gtcctgtgca ctgataaaac gggaacattg accgaagacc ggatcgaact cgtgcggtgc
     5761 gtggacggcc tcgggaagga aagcgatgcg gcgcttttct ggggccagct gtcttccacg
     5821 tatcgcacgg gcgtccgcgg tatgcttgat aaggcgatca tagaatacaa aaaatgcgat
     5881 atgcgcgcgt gggagaaagt ggacgaaata ccgttcgatg cggagcggcg tttcgaatcg
     5941 gtcgtcgtga aaaacggcgg aaacgtcatg atgatcacga aaggcgcgcc ggaagagata
     6001 ttgaaaaacg cggcgtcgta cgggttatcc cccgaagctt tctccacgaa aaatccggaa
     6061 gacgcgaaaa accatgcgct tactcccgta atgcgttcaa aaatggcggc tatctatgac
     6121 ggattgagcg ccgatggttt tcgcgtgctc ggcatcggaa taaaaaaagt cacgctcaaa
     6181 accgcctctt ataccaaaaa cgacgaaaaa agtctttcat tcgtcggttt tctcgcattc
     6241 ctcgatccgc cgaaaaaaag cgcaaaagcg acgcttgaaa aaatggaagc gcataacgtc
     6301 gcgatgaaga tcatcacggg cgacaatttt ctcgtgacaa aaaaaatagc gcaggaaata
     6361 ggactggcaa tacagggcgc gttcgcggga gatgcaatcg aaaaaatgga tgaagcggcg
     6421 ttgcggaaaa atgtcgagcg ggcgaatatt ttcacgcgcg tcgcgccgga agacaaggaa
     6481 cggatcatcc gcgccctgcg cgcaaacggg cacgtcgtcg catatatggg tgacggcgtg
     6541 aacgacgtgc tttccatcaa agaagcggat gtcggcattt ccgtggacaa cgcggtcgat
     6601 gtcgcaaaaa aaacggcgga catcatcctt ctcaaaaaaa gcctcggcgc aatcatcgac
     6661 ggcatcgtgg aagggcgtaa aacattcgcg aacacgttca aatacctcat gatggcgctc
     6721 tcctcgaatt tcgggaacat ggtcagcatg ccgatcgcct cgattgtcct gccgttcctg
     6781 ccgatgaccg cgccgcagat cctgttgaat aattttcttt acgattcgtc gcagctcgca
     6841 atccccttcg ataacgtcga gcctgatttt cttacgcggc cgaaaaaatt cgacatcggt
     6901 ttcatgaaaa aattcatgat cattttcggt cccctcagtt catgcttcga catcttcacg
     6961 tttgccctga tgtggtatgc gttccatttc gcggctccgg cattccagac gggatggttt
     7021 ttggaatcca tagcgacgca aacgctggtc gtcatcatca ttcggacacg cggtgatttt
     7081 tggaggaacc ggccggcggc ggcgctcatc gcaagttcat tcggcgccgt ccttatcgca
     7141 tggatcgtgc cgcatacgat attcggtaaa atgctcggct tcgtacaact cggcatcgta
     7201 ccgcttgtta ctatcgcggg catcgtggct atttatcttt tttccgtcga atccgtcaaa
     7261 aaatatttct accgcgcgta cggcgggttg atcgagaaac gatagttttt tacgcataaa
     7321 aaacggccta tgatggggcg ttttcgatgg tcatcgtatg gaaatccctc ttttgtattt
     7381 ataaaaaaga gaccgataat aacatcggcc tttccgggca tgcgccgcca attctcttat
     7441 cttcttcccg ttgcattgcc gaaacggcgc gggccggaag acggccgcga cggaccggaa
     7501 cgacggcgcg gataagcatt acttgttcca ctgggacgaa ccgaagaagc gcggccactg
     7561 aaccgtgatg aagggcgaga tggaaaaaaa ggacgatccg aaagcgcctc catgcgggcg
     7621 ggcggaagtt cgggaagctt tgaaagaggc agcgcggaac ggataaggcg ttctatgtcg
     7681 cgcacgtcgc ttttctgatc cggcgtggcg aatgaaatgg catgccccgc gccgcccgcg
     7741 cgcgcggtcc gaccgatgcg atgcacgtaa tcctcggaac tttgcggaag atcataattc
     7801 aaaacaagtt cgatcccctg cacgtcgatg ccgcgcgccg caatatcggt tgcgaccaac
     7861 acgcgatact ttccgttccg aaacccgtcc aacgcgtctt tccgctggcc aagcgaacgg
     7921 ttcgagtgca gctccgcgga agcataaccg aaactgcgga ttcccgccgc tatctttttc
     7981 gccccgtgct ttgtgcggga aaataccaaa atggaaccgc ggtattcccc caatattttt
     8041 tccagcagcc gcgccttatc atgccgctca acgaaaaaaa gttcttgggt gacttttttt
     8101 gccgtggtgc cgggcggcgc aatctcgacg cgcgtgggaa gcttcagata ttcctgcgct
     8161 atttgaacga tattcgccgg catcgttgcc gaaaaaagca tcgtctgccg ctcgcgcgga
     8221 aggacggcga gaatgcgctt caattgcgga gcgaacccca tatcaagcat ccggtctgcc
     8281 tcgtcaagaa caagaacgcc gacctgcttc agggaaatcg ttttctgctc cagatggtca
     8341 atcagccgcc ccggcgtgcc gatcacgatc tgcggattgc cacgaagcgc ctgaagctgt
     8401 cggccgatgg gttcgccgcc gatcaggacc gcggtcctga tgccgagcgg cgtccccacg
     8461 cggcgaaata catcgctcac ctgcaatgca agttccctgg tcggcacgat gacaagctcc
     8521 tgctttccgt tcagcgccgc ctgtatcatc ggcacgccga aagcaagcgt tttccccgtt
     8581 cccgtttgcg cgatgccgat gaggtccttt ccttcaagcg ccgcgggaat acatttttcc
     8641 tgaatgggcg tgggaaccgt aaagcgaagc ttatcaagcg cttccaatat gttcggagca
     8701 atcccgagat tataaaaacc tgaaaaagaa ttcattatgt ccgcatgata gcacagtaac
     8761 ggcaaaatag cgagacaaaa acgcgcaacc catcgccgca cattccgtcg tcctcgccct
     8821 atggagtggc ggcttcgcct gctccacgag gcgaagatca ctaagggcgg cgaagggacg
     8881 acgaagaaaa aatagcttgc tataatcgat gcttgattgt aagcggtgcg atggatttcc
     8941 aaaaagagat aacgcgatct ttaagaatta tcttaaaatc cttccgtaca acgcaagcgt
     9001 cttttcgatc atgatatccg gtctgaactt ttttgccgtc tcttcggcgg ccgatgccag
     9061 gcgccgccgc aaagcggcat ccccaagtaa aagatttatt ttttcttcgg cttcttcgac
     9121 cgtgccgacg ataaaaccgt tctcgccgtt acgtatgatt tccggcgcca cgcccacggg
     9181 aaaactgacc ggcaccaaac cctgcgacat cgcctcgatc aagctgagcg aaaatccttc
     9241 atagcgcgag ggtaagagcg caatgcctcc ggcatgattt ttaagcagcg cgggaatggt
     9301 gtctttgacc gcatttgcga taagcgtatg attcgggatg ttatttttca tccactgcac
     9361 cagtttttta tttttcgaca ttacgactga aagctttttg acttcgggaa accgctcgaa
     9421 aagccagata agacggtcaa cccctttcag cttcagcgta aacggatcct cccctatcct
     9481 gccgagaaaa acaagcgcag gctcatcttc cctattatta ttattgccgc cgggcgtctc
     9541 gaaccaataa tcctcgattg cgttatggat aacttcgatt ttttctgcag caatcccgac
     9601 gcttatcaga tcttttttca cgatagcgga tgtggcaatg atataatcgg tgcgctccgc
     9661 cgcataccgt tcgatggaag cgatatcatc gagcggccgc cgcgttttta tgttcagttc
     9721 gcggatcgcc cccgccttct tcaggcggag aaaaacatca ctgcacccgt tctcaagatg
     9781 atttgctttc ggaacggcct gaaggaatcc gacggtggtc gagccgaaat gcgacacgac
     9841 cggcttggaa aaacggagaa aggcaagcgg cgtgtacgtc gttccctgga taatatcgtg
     9901 acgcaatatc tcgtcgcgtt tttccaacaa ggaatagaaa aaaagaatat tgttgatgcc
     9961 cttgaaatga accggcgtgt ccgcgagcag aaattgcgga taaaaactgt ttacctctgc
    10021 gcctttctcc cggagtttgc aggcaataag atcgttgatg aagtgcgcgc cggtgcctat
    10081 ctttttgtcg ctatggatga aggctacttt cataatcgtt ataatcccgc tctatcatcc
    10141 gtcaaggagc aatggacaaa aaaacataaa cgcgggcatt ggcccgcggg atatgcttta
    10201 ttatcgattc agtatatgcg attttcaaaa ccccgtcaaa gaaataatga tatgacaata
    10261 ttgtttgtga tttttaaatt gaattgttat actttccgcc aaaggtcatt taaaaaaata
    10321 aaaaacttct gaatatac
//
LOCUS       DAIDDT010000027        10203 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_27_length_10203_cov_6.934327, whole genome shotgun sequence.
ACCESSION   DAIDDT010000027 DAIDDT010000000
VERSION     DAIDDT010000027.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27_length_10203_cov_6.934327"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2485 a         2418 c         2580 g         2720 t
ORIGIN      
        1 acccgatcga gggtcgaggg cgacggaaaa gaaggccgct ttcttataag cgaaggtcgt
       61 aaccagaaca agctttatta cgttctacat gatagatggc gaggaaaaac ttatagatcg
      121 cgccgtcggg ggagattcct cggcttttgg cgttctttat gatcattatc agccgatgat
      181 ttatcggttt acgctggtca aaacggggcg tcgtgaggag gccgaggatc tcacccatca
      241 gatttttttg tccgcatggc agaatgtcag gaactacaag catcgcggac atcctttttc
      301 aagctggctt tatcagatcg cgcggaatca ggtagtggat tattaccgtt cgaagaagaa
      361 taatgcgagc cttgatgacg gtgaaccgga gcttttcacg ccttccatgg cgacggatcc
      421 ggtcgatgtt tcggtgaagc ttgccgtgga gaaagttttt ctcgcgataa aaaaactgaa
      481 accggagcat ggcgatgtca ttatgctgcg atttgtggaa gatctttctt tgcgcgatac
      541 ggcgaaagcg ctcaagaaaa cggagggcgc cgtaaaactg atgcagcatc gcgcgataaa
      601 agaattgaaa aaaatattgg gcaattcgat cgcgcaatta taaccggaat taaaaaatat
      661 cggcgatgcg gtttcgcaac tataaatcaa tggaactttt tgaaatatta aaacagtgcc
      721 ggcttataaa accggacgag gcgtttaccg aacgctcgcg ccgcgccgtt ttggcgtcat
      781 cgcagtttga aagccagacc tcgatgggga ttttcaaggc gcgccgcttc attttccggt
      841 tcgtcgaaac gggcgccgcc cttgcgctcg cggggctttt tatcgtgctt atcatgggag
      901 gattttccgg aacggcaatc gctcccgtcc ggttctccgc gattgatccg aaggtgcttc
      961 gcgccgaagc gcaggcaatc gacgcgcaga tcaatcttgc cagtttgaat tattccgagc
     1021 ccgccgcggt cgcggaatcg acgcagaaaa ttgccgattc tctccataaa gacaaaaacg
     1081 acaaagcatc gagcatcgct cccgcaactt ccactgtttc tgttccctca agcacgaatg
     1141 acgtctcctc atctttctcc tcatctttct cctcatcgac cctttccatc gaccaggcgc
     1201 ttgaagaact gacgaaatag aaatttcatt ttctctgcca tcggccgttc ttttaaggta
     1261 aggtaaggat taagaaaaaa gaatggaagc atgaagaatg gaaagactgg aagcatggat
     1321 ccttctcttt ggtttgggag gacgacagat gagaagtatg aatcgtcctt ttcggcagta
     1381 tgacagaggc ggtttttgtg ctataatgga ttattatgaa aaatataagg tttgccgctg
     1441 tggcggcgct tgcggtattc ggttttgcgg tttttgcacg gttgcctatg gcgcgcgcag
     1501 cggacaactg cggtatttct aaggatgaca tcgccgcgat ccaggcaatc caatcgaatc
     1561 ccaacctcgg ttatcttgac gagataaacc aggagcttgc cgcgcgaaaa aatcttttat
     1621 caaaaacgat cacgtgcgca aaaaacgacg cccaatcttt gcgggcgacc atggcgggcg
     1681 cttccgtaag cgcggcggat gaaaatatta aaagccagct tctggggaat atagacgttg
     1741 ccatcgatta ttacaatctc gaacttggaa aactcaacga ttcggggata aaagagaccg
     1801 aacaaatcgc gcaggaagtt ctttcatggc gcgcgagcac ctatgtccag ctcgccggcc
     1861 gcgtcgataa ttttatttta tggtcggaga atcagaatct ttttgaaacc gcctcggcgc
     1921 ggatggcgca aataacgccg atggtttctt tcttgtccca gacgaacaac agcgaccttg
     1981 cgaacgcttt ttctgcggcg cagacgtcgt ttgccgccgc gaaaaacgag aacgcttcgg
     2041 cgcgcagcgc gatcgtgcaa tcattgtctt ccgaccagac gctcggcgtc atcaagcagt
     2101 ctttgcaatc gctttccgac acttatcaga attttttcaa cgtgagcgac atcatccaga
     2161 aactgctcac gggaacgagt cagagttgat gggaaccccg tcgtttttcc ttgacctttc
     2221 gttatttatc catgccgaaa acaaaaccct gccagtaaag ctgatgggga gtttcatttt
     2281 tccctttcgg gttacggcag gttaagcaag taaccgggat gaatgttttt aatcacgcta
     2341 agccggggaa gaaatcgatt tttatttttc ggcgcggaac gcccatggca cggagtattt
     2401 ccaggaaagc ggcgaccatc gcgggagggc cggaaacaaa gaagagccgc tcgcggtagt
     2461 cggggatttc ccgcgcaatg atgccgggac gcaagggtcc ggccgagatt ccggaagccg
     2521 gagcctgttc tctggtaacg caataaaccg tttttattcc cagttcgttc tccgcgcgat
     2581 ccaaaatgtc tttgtaggcg atttcttccg ccgtcttatt ggaataaata agcgttgccg
     2641 tgcgggcgtc tttcgtatcg agcatgtatt tgatcatgct gcggaagggc gtgataccga
     2701 tgccgcgatg aacaaaaaag aaaaaattgt tttttcatgg tacagcgtct tattatacag
     2761 gacgtacgca cgtgtgccgg gtacggggca ttttccgggc actataacca tagtactgat
     2821 tactgatact gtatgaaact cggttgaggg ctgagtttca taacctcggc gggcgtcatg
     2881 agatggccgg cacgggtatc gtttttataa aataatttga aaccggtgaa ttgcacgggt
     2941 tcgctttcga taaaatcttc atacgttttt atcttttgcg caggcggtcc gaagccgtcc
     3001 atgtccatga caatctccac ctgcggcaag ggggttatat cttgataatg cgtcaccatg
     3061 ggctgagtaa agcggtgtat cacgagaatt ttcggcggca gattattttc tttgacgata
     3121 ttcgccaggt aatcagccgc gtaattgata tccgatgcgt ccatggtgcc gatcacggtt
     3181 ccgggctttt cgccgttatg catggcaaat tcagggtcga gcgcgagatt tacctgcggc
     3241 aacttaagat acggctcaag aagcggaatc tcattttgca catcgcttaa tccgacttgc
     3301 acgtcaagga acacgatgcc gttcatttgg gacgccatct gaatggcttt ctctatctgg
     3361 ctcggaggca tgcggaggcg gtaatcgccg tccggtccag gattattctg agcggcaacc
     3421 gcgatataat cgagcgcggg tatcacgggc gttgatggat cggcagtttg ccatgcctgc
     3481 gtggtactcg cgagcatttg caatacctgc gccggcggat attcgccaag aacgcccatt
     3541 tgcgtcgaat agaaattacc gtaataggcg acaatacggt cgaatggcag caacgcgccg
     3601 ccgtcgggat agatggtttt tacgggccag ccgaccggca gggtcgatgt cgtgatgata
     3661 gtcgatgtcg gagtgaattt tgtcccctta ataaaggtca cctgcttgat gattttaaga
     3721 ttcgccactt gaagcagttt tgcgttatat gcggcgatat cgagcgacgg ctgcggcgcg
     3781 agatcggaag gcgttgttac ggtattcatc attgttgttg ccgaaagcgc cgccgtagcg
     3841 gggtcgttgc cgccgtcaat gacgatcacg cggggatagt ttatcgatgc aaaaacgccg
     3901 ccgataacgc cgaggaaaat aatgcccata acgataccaa tcctgatttt tttattcatt
     3961 attcattcgt atcccaacag cgaacttttt gaagaaatgc tcaaattgcg agtatcgacc
     4021 cgcgagaaac caacggaaaa tatgaagagg gttatattaa ggggtcaata ggggtcactg
     4081 tcagaacgat gccataaccg cactttacga ccaggaatat atggcggccg tgagagaaaa
     4141 actgaacatt atcgcggcgt tcaaggcggc gatggcgaaa ccggtgaaag aataaaaaat
     4201 cttgcagaaa tcgggaaatg ccattacgaa gccatacggg tcgagaaaac ggggtctcca
     4261 tgggtgcgct atagcttata agaaactgtt acgaaatcgg cgactcaatt tctggatttt
     4321 ccgtagcgct tttggatatc atacaacttc caaactcatt tctcaatctc gattttcatg
     4381 atgagcggat tcagctctgc gtgctcggcg ctgagctttg ctttgccttc tccgttcacg
     4441 gcaggatgag caataatccg ctgatcctct gtttcatggt cgccacgctc atcaagccga
     4501 agcttcgcgg tcgctttcgc gtcgtgatgc cccccacatc caggtcgcgc atgaggatgt
     4561 cgacgtattc tccgaccgag ggatgcagga gttctccgca gacgcggttg ccggcctccc
     4621 agacggtttt gagcgccgca tcgacatcgg cgccgtagac gattttctgt ccccgtccgt
     4681 cttcatgagt cggatccttt ctttggatcg ttctgaattt ccgaatagcc gcttttctcg
     4741 tcatctttgc cacccggcag accgcatcca atatcgctcc tttttctttt ctgtcgctcc
     4801 gccaatacgc cgctttatac tcctcgaata tgctgttttt ggtttccata gtcatgatga
     4861 ctacggtaac ccatttatga gctatccact cctatttcgg taacagtttc aatgagctat
     4921 aacgtgcgtt gattttttcc actgcgactg ctacactgta aacgatatgg taaaaacgca
     4981 acgacaattc acggcggtat ttcgcaaggc ggggaagcaa tatctcgctt ggatcgaaga
     5041 gatacccggc gtcaacacgc agggcaagac gctcagggaa gccaaagaga atctgaagga
     5101 agctctctcg ttggttttag aagtcaatcg aaccatctcc tctgcggaga attatggcca
     5161 agatctgatc cgggaaaata tcgcaatcgg agcataaacc tttttaccca atgaaacgga
     5221 aggatctggt tggccatctt ttaaaggagg gctgcgtcca agtccgggaa ggcggacggc
     5281 atacggtatt cctcaaccct cgcacgaaaa gcgtttccac cgtgccacgg cacactgaaa
     5341 taaacggata cctggcaaag aaaatctgtc gggaccttgg ggtcaatcaa ccaagaaaga
     5401 aataggcgtc ttgaaagtgg gttttcgtat tcgtatatga gatgcgaatt gcacgaaatg
     5461 ataccctatg accctgtaaa ataagggtat aggggtacaa aaataaactc ctaattgaga
     5521 gagggtcaaa atgccaatta tacaaaatcg gcaaaaaata agcggttcca agttggcacc
     5581 ggaagtgccg aattaggaca ccccgttttg gcggtgtttt ttgttttttc ttttttttct
     5641 tccggtgttt cgcgagcatt tcgtccggcg tgaatgaatc aagacatccc attggtcgcc
     5701 gactgagttt tgcttcaatc tttttaatac atctttttga atactttgat agatcgcttc
     5761 ctttcggaat atcttcgcga atcacgccgt tcagatgctc gatacttcct ttctcccatg
     5821 aatgataggg atggcagaaa tagatggtaa tattcaataa tttctcgaga tgtttatgat
     5881 gctgcaaaag cagatcattg tccgtggttg ctgtcttcat ctcggggaaa cgctgcttga
     5941 tacggagaaa cgccttttca aaatttctga ttgaaaccgg aaacactttc tcgatgaacg
     6001 gcttccgtat ttttcgatca accgcggtaa gcaggatgcc tttccctgat tttcccgaaa
     6061 cgatgaaatc gaattcaata tcgccgaccc ttcctctctt gtccgctatt ttcgggcgtt
     6121 tttcaatgaa tttcctgtct ttgagctttt ggtggcgtcc tcttcgagga cgccgtttct
     6181 ttttcctatg tacccaaagc cagttcgcca tctgcgctcc cgaagcgctt ttgagccatc
     6241 gatagatggc atctttcgat atcgaaggaa gatgcttttc tcgttttgtg acgcgtcggg
     6301 cgatgttccc cggtctttgt ccgtccttca ggcgggcaac gattgcctgt ttcagttcgg
     6361 gatgatcgat gatcttcttc ccttgatagt gcgcgtactt tctccggaca tacgttttgt
     6421 gctgcgcttt cttcgggcta taccgccttt tcactttgtt gcgccggact tcaagcgcaa
     6481 tcgtattcgg acttcgttcc atcagctccg cgataaaacg gagggtatat ttctttttca
     6541 agaatccttt gatctcgcgc cgatcgcttt ccgtcagttg cttccatgat ttttttgatc
     6601 attgtttttt ctcatattat cgcatttttc ggcgaccgac tgtcctaatt caaaagagaa
     6661 agcgggggag ggtgctcatt atggaaaagg acatcttggg cgaggggatg gagttgacga
     6721 aggtcactct cgaatcttcc tcagagagca attgcattcc gcagtgcggt cccgattgcg
     6781 gccccgcctg cattccggcg taagccggga ccaaaacatg gcgtcccttt tacgagggac
     6841 gctattatct tcttttaatg ccagtcggcc cattcggtaa ttttagatcg tggttttaaa
     6901 agcccttctc tgtgaaattt tttccgcatt cttttttcga attggaatat atcctcgttt
     6961 ggatttttca gatacttctt ccaatcggga aggagttttc tgttcaagcc ggcaagatac
     7021 ttatgtccca ttaaaatatc gttttcctcg aaatatttct tactgtcatt caatcggatg
     7081 cgcagttcat tatcgcagga aagttcgata agcgcgtgca tcaaatgcgg cgcaacgggt
     7141 atccccgagg taatatgatg cattatttca tgccataaat ataccgtcgc gtagtttttc
     7201 cattcgtcgg gatgagacca tgcgatggcg ttgttctcca cgtacgttct tccgcgccag
     7261 agcttcggat gcgagacata cacttttatt tctttattga tcttggatag atctattttg
     7321 gttatgtcgg taagatgcaa aagggaaaaa tcataattct tattccattg tttctctacg
     7381 gtcatcgcat atcgttccgc ttcgcggaat atctttttga actcggcggt tccatagatt
     7441 gcggaaagat taccctttag attggagaat gccatggaaa gccctttcgt aagcccatcg
     7501 tcaaaaataa gcggttccaa gttctgggac agaacctcat agagattctt atttgtatcc
     7561 caaaccttcc ttttcagctt taaaaaggga agataatcgg aatggctttt gagcgcgtgt
     7621 tgcaggacga agtctttatt tgcgacgaat ttcaacgtga tcattcttat aattataaga
     7681 acttgacaag aaaaagtaaa agcgttttct ggtcaatagc ttggaatagt ttcggcgatt
     7741 tttttgaacg gcgtaaggaa attgataccc atgtgactcc gctctccgtt gtagtaagcg
     7801 aggtaaatct tgatggcttt ccgataggat ttgcattcct tcggaatgcg atcaagacat
     7861 tcctcctgga tcgtgcggtt gaaccaacag tcttgcgtaa atataagaat tgtggcaata
     7921 tatgtaacag aaaaccaatg atggtttcta tggagcagta cttttaaaac aaaataaaaa
     7981 ggaggcgaaa agccatgaga gcaaattctt tgccgttgtt caacgtaccg gcggtaatct
     8041 cgagtcaccg aaccatattc cacgcgttcg atgagaaggt tatcgtggaa gctcccggca
     8101 atcttataag gaagcttgtc gaactctgtg acgggagcat taccttcgaa gaggtcatcc
     8161 aacgcatcaa aggagaatgg gacgaggaat cgatgagagg gcttattgag gatatgtttc
     8221 aaaaaaacct tcttattaac gcgcgcaatc tcggcgaaga agcatggaaa gccatagaaa
     8281 atccgatgcg ttttccgatg attatcggtg agaaggatgt cgtagaactt gttcgtgttg
     8341 caaccgaacg gcatcgttcc gattcggaca agtgtcctta cagggcgaaa acaagttcgc
     8401 acggatcgct gatcgctcaa cgtcaatcca ctcgttcttt ttcgggagct ccggtgccgt
     8461 ttcaaagcat cattaatatg ctctggagcg catacggaga gtatgccacg aaggatggta
     8521 gttcgcacag gacgagtcct tccgcaggcg cgttgtatcc gcttcttatc catgtcgctt
     8581 tatttaccga aacagacaag ctgcaaccct cggtgtatcg tgtgtgctat gactcttctg
     8641 ggaatgtcgg actgaagctt gcttcgaagg acgtattgca gttcgcgcgt tcatttctta
     8701 accctgcggg cattttgaaa ggtatccatg gagttatcgt gatcagcggg tcattctcca
     8761 taacgggaga aaaatacggg aatcggagca tgctgtatgt gccgctcgaa gcgggtcatg
     8821 ccgctcaaaa cattcttctt gaggcagtcg agcacgatgt tgccaccctt gagatcggcg
     8881 ggttcgtcga taagtttctt ggagaagcaa tcgatctgcc gctgggatat cggccgctca
     8941 cggcgatcgc attcggggaa aaggcaaacg cattggatat tgtagttccg aaatcgacgc
     9001 tggaggttga atgggcgatc cccgtagctg gccagtactg tccatcgttc gctatcgctt
     9061 cggcgcgagt gtcggctcaa cgcagctggt cgcacggaag agatgcatct ccgatcatag
     9121 cgcttaccaa ggccgttgcc gaagcgaaag agtgggcagc atgcgggcgt attcccgatc
     9181 tggttcgtgc gcgtttcggc gaacttgaat cggcggttga tccgcgagat atcatcaagt
     9241 tccatccggc gcagtatcgc gtgaaatatt ttccgttcgc gcccttcgat gaaaacaggg
     9301 aatacgcatg gacaaagggg tacgaggccg tgacgggatc aaaggcatat attcttgctg
     9361 atcacgtgta ttttccgtac ttccccgata cgccgtatta tgccttcgca aattcatccg
     9421 gatgcgccgc gcaccccgac gaacaaacgg ccatcgagac ggccacgctt gaacttgtcg
     9481 agcgcgatgc tttcatgaac gcttacttgt gcaagctcgc gctccctacg gtagaggaga
     9541 agacatttcc cggcgatatc cgaaagagga ttgccgatct ccaatcgctt ggcttcaagg
     9601 tgtggatcaa ggatcactcg ctcgattgcg cgcccgtggc atttgtgttt gctcaaaaca
     9661 aggaacttgc gtttactacc tgcgcttcgt gcgcaagttt tgacatcgcg cacgcggtga
     9721 gccacgcctt gatggaagtc gaagcgtccg tgctatcgcg cctccagaac ggaccggcga
     9781 aaccgacgag acccgatgaa gtcgtgatgc cgcttgacca cggaaggctc tacggacaga
     9841 aacagtattt ccgcaatgct gatttcctgg ttcattgcgg agatcgtatt tcattcggcg
     9901 agatcggcgg caacgtcgcc cattcatgga atgaactcct tgatcgattt gcctacaagg
     9961 gatggcgtct ttttgtgatt ccactccatc tctcggatga gtacggcggc aacggcgact
    10021 tgtacatcat ccgttccgtc gtgcccggca tggtgccgat gacgttcggt tatcggcaag
    10081 aaccggcagg catggaacgc atttacgcga ttgccgaagc aatcggaaac aaaaagcttt
    10141 cctatgggga gcttacgaaa tttccgcatc ccttcgagtg atcgctgggg cgggaagtaa
    10201 aac
//
LOCUS       DAIDDT010000028         9408 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_28_length_9408_cov_5.750080, whole genome shotgun sequence.
ACCESSION   DAIDDT010000028 DAIDDT010000000
VERSION     DAIDDT010000028.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28_length_9408_cov_5.750080"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2201 a         2627 c         2161 g         2419 t
ORIGIN      
        1 ttttttatct tcagcgaatt acagatatct ctttcttcaa aaatactatc cagacagccg
       61 ggtaaatatc gcttcgcttt ataagacatt attcgtatgg ttatatccga catgttctca
      121 attataatcc atttcccgtt attatgatcc actcaccgca atctgcccgg gatcacttat
      181 cactatttcc actttgccgc cataactttt tattgcgccg gtgatgtcta tttctttgcc
      241 ctgatattgc gaaacgtttt caaactttgg cgcgtccgac gcaaagatca ccgcgctgaa
      301 cggacagttt ttataatctt tgcaataatc gaaaaacacc atgccgccgg tcgaaatata
      361 aatatcgacc ggcgcgccgc gaacggtcgc cgtctcgccg atgtgagccg ccgcatcgaa
      421 ataaggataa acttttccgc caccggcgcc ggttgcgccc ggcgcgccgc cgcgggctgc
      481 gttttgccga ttattttccg ttccgttccg cgtgtccggc gcgccgtcac tccccctgtt
      541 taccgttaaa atattcttga tgatgtccgc gtactcgccg cgcagacgat cgtcggtccc
      601 cacttccaaa agttcgtcgt tcgcgaccgt cgccgactcc gtccagttat aactgcccat
      661 cgcatatgcc ttgtccgtca cgatcgcctt gatatgcatc aggccgtccg catgtttctg
      721 cgtttcgacc gcgatacccg cggccgccag ttcctgaatg accggctttt cgtatgcggt
      781 tacggcttcc cccgcatcgg tgatgccgcg cacatcgatg ccacgttgtt tcgcggcaac
      841 cagcgcgtcc gcgatattgc ttttcgtaaa aacgtaaacg gcgaaataaa tatatcgttg
      901 cgcgccgttc acaacatcaa tgatcgcctg atcgttcgcc cgcttgtcca gactgtaaaa
      961 aatcgccggt tcgtttgatg taaaagcgga agaaggataa aaatgtcccg tcaaaaatcc
     1021 gccgagtgcc gccgcggaaa caatagccaa cactatggcc gcttttttta ttgatacgaa
     1081 agcaacacca agcgctatgg ccgctttttt aatcagtgga acacgcatcg ataaaatcta
     1141 ccgcgcgcca cacgttttac aaagcggtct ttaagaaaat tttaatcaat tttcaactct
     1201 cgtctccttt gttatcctca ggcgtaaata tttttttctg tcatcctgga aaccgtcctc
     1261 ggttatccag gatccaggtt tgaattttat attttgggaa tcattaagaa aaaattaatc
     1321 tggatcctgg atatttttcg aagactcaaa gttccaggat gacagaatga aaaaaacgac
     1381 caataatcct acgcgatcat ttttttgaat tcctcaacca tcggaacctt ctcggcttcc
     1441 acgccgtaga gcttcgcgag atggaatgcc gtccattgcg ccgtattgat ggaagaaaat
     1501 atctttgcga tgcgatgtcc gccgcgaaac tcaacggcgg aaaccctgaa cccgcgcgca
     1561 gcgaaaagtt tctcgatcac tttcatcctt tttgcgatcc gcggatcgtc gtcatcatcg
     1621 cgcaaaaaaa taaaatggaa tttcgcggaa aggccgcggg ttcctttttt cacgtcgaaa
     1681 ccggtcatct cgttatgatt caattccgga aaaacgttcg caaacgcggg aattttcccc
     1741 gtttcgttca aaacgatttt ccaattccac gcgacaacgc cgttgcggcg cgacgcgtaa
     1801 ataacaggaa cgctgccttt taattttttc gccatttttt ttccgatagt ttcggaatgc
     1861 gccggagtaa gcatatgttc aagcgacgta acttctttca acgccgaccg ttcgcccata
     1921 agcgcgagta tggctttcat tgaaaacccg agcgccatac gcggctggat ggatccgtcc
     1981 ggcataacga cgtacggcac gccgaaactt ttcgcgagcg agagcagttc accgccggac
     2041 gccaccgccg taagcggcaa acgccgctcc ttcgcctcga ggaacgcgct gatcgtttcc
     2101 tcggtatcgc cggagtgcga actcgcgatg ataagacact cttttttatc gcgttcggaa
     2161 agcggcggca ggccgtaatc gttccaaatg acgatgttcc attccggttt ccacgcgctc
     2221 aaaatatccg ccgccagatg cgacccgccc atgccgcaca cgatgatttt ttttgcgccc
     2281 cgcggcagat gcgccgcatt taaaacatcc ggctcccatc cgaactgctt attgaaatct
     2341 tttatgtcct gtaaaatcat cgttccatta tatactttta ccgcggattt aaaaaaggat
     2401 cgttgcgccg gttatttttt cttattttta tttttatcac cgattgcatg gccgccgaaa
     2461 cgattgcgca tggcggaaag gaccttcccc gtgtagctcg gcgatttttt cgaacgaacg
     2521 cggaatttga aagcgtcctc tataacgggc agtttcattt tcattttttt accggtcttc
     2581 acggtccact cgccttcgcc ggtgtacgcc accgaaccgg acaccgtttt caagtctttt
     2641 ccgtaaatgc ggaacgcatc ttcgagccag ccgaccagcc gcgattcgac gacgctcccg
     2701 cggttataaa cctcggcaat tttccggaca tcaagacgga actttgattt tttcataagc
     2761 gcgaaccctt cggcgatcga ctgcatcatg ccatactcga tgccgttatg caccatcttc
     2821 acaaaatgcc ccgcgccggc ttcgccgaaa tattcatatc cttgcggcac ggaaaggtcg
     2881 cggaaaagcg gctccagata ttcgaatgtc ttcctctcgc cgccgatcat cagcgaaccg
     2941 ccatggcgcg cgccggcggg accgcccgaa aatccgacat ccataaagcg gattcccttt
     3001 cttttgaaaa tttttgcgcg gcggatcgtg tcttcataaa acgaattgcc cgcgtcgatt
     3061 acgatatctc cttttttaag gtgtccagca atgccgtttt tgccgaataa attttcatca
     3121 actccttttc cggccgtaat cataatccag acaatccgcg gcgattttaa tttcgcagca
     3181 agatcagcgg acgaaaaggc gcccgcgata ccttcccttt ccatttcgcg cacgggctcc
     3241 ggcgaccggt ttgcggcgac aacgcgccat cctttttcat gaagctggcg gaccacgcct
     3301 gcgcccatct tgccaaggcc gatgacgccg atttccttcg gcgcaacggg gatgttccgc
     3361 gaccgcgtcc ggtcgatggc gtcggcttct ccggcgatgg cgtcatggtc gggagcgtaa
     3421 tgccggagcg gcaccagctt cttttcccat gcggcgataa agggatcggt gaaccgccac
     3481 atcgcggtga cttcttcgct cgaaacaaaa agggtctgat cgcccgcgat gcaatccaaa
     3541 agcaattttt catattcttc ggtgtactgc gcatgagctt cttttttacg gatgccggcg
     3601 gcgggaacgt tttcgtcccg aaaatcaaac gcaagcgacc gctcctcggt ctcgaacgaa
     3661 tatccctttt ttttcgaaaa gaacgcaatc gtaatccctt ctttgggctc gagacggatc
     3721 acgatgcgat ttttttgatg attgccgtcc ggacaaaaac aaggacgccg atgacgcaga
     3781 atgatttcga tttccttacg cgctatcccc atgcgcttgc ctgattccat cacgacggga
     3841 atgccttccc atcgccgccc cgcaagatat ccttttatct tgaaatacgt ctcggtttgc
     3901 gaatccgccg caacggcttt tatggaccgg tagccgtcgt attgcgcgcg gaacgacgat
     3961 gccgccgctt cggcgacggt cggtattttc agcgtgcgca aaagttccgc gcgggcgcgc
     4021 cgtattgccg cggcgtcata ccgttccggt ttttccatcg taatcagcgc aagcatcgca
     4081 agaaggtggt tctgcccgac atcgcgcagc attcccacgc cgtcataaaa tccaccgcga
     4141 tcttccacgc caatgctttc aaaaagcctg atattaatcg cttcgataaa accgcggtcc
     4201 cattcccctt caaaaagatt gtttccgaac cggaatgcaa gaatattctg gagcatttcc
     4261 ttggcgagat aatggtcgat gcgataaatc tgctcttctt taaaaagcct ggcgaggcgc
     4321 gcgtccagcg ccttcgcgct tttttcgtcg ttcccgaacg gtttttccac cacgatgcgc
     4381 gtccagcctt cgtccggtcc gcacccgtcg gcaagatggc tcgcgtggat gttttcaaaa
     4441 atcacatcat aaaactgcgg atggaccgaa agataaaaca gtttgttcgc gcacatgccc
     4501 catgctttat cgattttttt caacgcatca cccagtccga cataatcgga acgatcgttg
     4561 aattcaattt tatggtactg cgccaattcc aaaaacgacg ccacgtttgc cgcggacgcg
     4621 ccgggcgcct tcacggcaag aatggtggcg atgtgttttt tcaggtcttc atcggtccag
     4681 ccgcgccgcg aaatgcccac caatttgaaa cgatgcggca gtttttttct ctcgtgcaga
     4741 ttaaaaagcg ccggaacgat ctttcttgcc ataagatcgc ccgttgcgcc aagcactacg
     4801 aaaatagtgg gaacgtttgc ctttttgttt tccataaatt taataggacg cccgcgctgg
     4861 cgccgctacg gtttgagaca ttttcttatg attttttcaa atgcgcacgt cctattttat
     4921 tcttaacggt ttattgattt ttattcgcaa tctccacctc gatggtatcc gggtcgattg
     4981 cggcaagcga aaaaccgtcg ggaatctccg cgtcggacgg ttttaacgat acgtcatgcc
     5041 atcctggctt gccgactgaa ctaagatcga cgacaacgtc cagcgactgg ggcgcgagtg
     5101 attcgaaact aaggttttgc ccctgaagcg tcagcaccgc ttcgcgcggg atgacatcct
     5161 ggatgacgta cgtcgaagaa acgttctgaa attcaagcgg tacggtgaat ttcttctgga
     5221 ccagggtgaa ttcagggacg aaaaaaagcc acacaataaa cgcaatcacg accgcaatgc
     5281 cgaaagaaag cgcgttccgc gccgtccact gggaaaaata actccaacgg ctcccggaaa
     5341 caaccgcgcc gtaaaacgcc gaaagttttt cacggaccgc cgtttcgtcc tcacaccgct
     5401 cgaatgcggc atggcgcgca atgtcgattt ttcccgattc tccggaaacg acgacaacca
     5461 gcgcatccga gcgttccgaa agtccgatag ccgcacggtg gcgcaaccct gattttccca
     5521 accgtttcag attttcggca agcggcagat gaaccccgaa ccttttcagt ttattgtctt
     5581 cgatgatcac ggcgccgtca tggccgggcg tcgattcgtc gaaaatgctc ataaggagct
     5641 cttcggaaat actgccgtga agcgggatac cgccgtcaag catgcgcgcg atcgattcct
     5701 tgcccggaaa aacgatgagt gcgccggttt ttgtctgcga taaacgcatg accgcgcggg
     5761 caaccgtcgc tatcgtcgcc tcggtcggcg gcacatgcct gcggaacttg aaaaaaccga
     5821 cgaaagaaaa aaggcggcgc aattctcttt gaaaaataat ggcgataaaa ataagaatga
     5881 ctccgaaaag cgactgcagt acaagatgcg tgagcggcag attaaaaaca atcgatgcgg
     5941 catacagcac gcccaaaccg aaaatgccgg ccgcgaccga aagcgaatgc gtttcactca
     6001 agaccctgat cacgacatag atcaggacag cgatgataaa aaaatcaatc cagtcgttca
     6061 cgcccagaga aacattcttg aagccgttca agaacgccac gtttgccaat agattgccgt
     6121 gcaatatcat gtttttattt tacaggattt cggaaaattc cggaaatcga atcggaaatc
     6181 gaaaacttac ggatgcccgg cattattttg tcaaacggtt gtccgcacag cttgccctga
     6241 atttgtcgaa cgggaaacgg atccgcactt ccggattttt ttaaaagcgt ggctccataa
     6301 tcataacttg gacaacaggg aaaagccggt tttaaacgtt tcgaacggtt tcaatcaacc
     6361 aaaaaaatag aaaaaactta ccggtggaac ccgagatcgg cgggattatg cgcgccggaa
     6421 attctttcta tcacaccgcc gtttttttca aaggcatccc tcatttcggc gacttcgtgt
     6481 gcgagccgga cggtttcttc gggcaattct ttttttttca tacgccatcg tgaccaataa
     6541 tcgataacca gtttgctgtc gccgaaaata ttttttacgc cgtcatggtc cgccactatg
     6601 agcgcgtaac gcaacgccag aagctcgccg taattgttcg tcgcggaaac gcggtcaacg
     6661 cgatgctttc cgaagcggtt cagttctttc gccgcaagcg ccttgtgaag caggtttttt
     6721 cctttttcgt cggtcacgct tatttcgacg ccgtttcccc tgccggttcc cgcatcgaaa
     6781 taaattcctt ctttaagttt tttcgccggc ttcgcatcgt agcgcgcgcc gctttcgagc
     6841 cattgtccgg cctcttccct gtttgaaaat ccccgatagc gcgcgccggc gatcccggaa
     6901 actatttttt cgcatgccgc ccagcttccg gcgatccctt ttatctttaa ggacggaata
     6961 atgtatgcgt aaaacttatt tttggtagcc ataattgata atttttttaa tgccggtcgt
     7021 cctcgcccgc caggattttt ctccgaaaat tcgggcgggc gagaatccat gctttaagaa
     7081 tttaaaagag aaggtttgaa acataagcca ttcgtctttg cgaacgacga taaaaaaata
     7141 aaaatttctg acggattatc gcctaataga tttccatggc aaaggtttcc cgtcgggaat
     7201 ctcgaaacct gcggacgccc tatggccgcc gccgccgaac cgcgccgcta tcttcgcgac
     7261 atccacggtg ccgtcggaac ggagcgaaac gacggtcatg cccggccgct tgttccagat
     7321 gatcgccacg ggaggaaacg cggcatacaa cgcgtgcccg atctcggatg catattgcgc
     7381 ggcattgacc gcatagattt catgtcccgc aaataatact ttctccgcgc ttttgccgat
     7441 gatgcgggct atggccttct cctcataaca cagcacgagc ctgccttttt caagatatgt
     7501 ttttcgtttt gaaatatttt caaaatcttt cgcaaattta tcccaaagac ggaaatcaaa
     7561 atcatagaga tcaagaaatg cataaacggc cttcgtgtgc ggcattttaa atttccaaag
     7621 atccatatcc tcgatcgtcc gtaaaagcca gggtactttt ttaccgggat ggaaatagcg
     7681 ccatgcaagc accgcccccg agtggttcaa cgaaaaaaga ggattgcaga cgagcgccgc
     7741 gctttctttc gcggaaatat gatggtcgat gatcgtcacg cgccggttat cccggataag
     7801 ttttttcagt tccgccgtcc ggttcggcgt aaaatctata aaataaagtt cctttccttt
     7861 gagtcccgcg ggaagcggtc tgccgcggaa gagcggcaca taatccgctt tatctttgaa
     7921 ttttttccat gccgcccatg ccgcgccgaa cccgtcggaa caatcgccgt gataaagcac
     7981 cgctatattt ttctttaccg ttcgctggtt gatgcgattt tcgatgaatt tagtcatgat
     8041 tttaaaatag gatgcacgcg cttgtccgtt tcgacttgaa gcattataac tccccgcacc
     8101 cgtcttttcc gtcgtcctcg ttccgtcctc tctgtcgtct tcgcggaaat aagaatcctc
     8161 cccctccgtc gtcctcgcga agtcaaggat ccacgcttcc cgtcttttct tcttaatcgc
     8221 tcatctctct ttttcttatc gttatttttt tcgaggcgcc gcttcgccgc agcgcctcga
     8281 tgcccgacaa tttttctccc gccaaaaaat caagcatcgc gccgccgccg gtcgagataa
     8341 aaccgaattt tttatcgaaa ccgtaatctt taaggaactt cacggtctcg ccgccgccgg
     8401 cgacggaaaa cgctttccgg tttttcacga tcgccgcggc gatagaaaaa cttccgtggc
     8461 gatattcttt tttgtcggtc atgccgagcg gcccgctcca gatgatcgtt ttcgcgccgg
     8521 caataacgcg gctgaacttt tttatcgtcg cttcgccgag atccacgatt gcgttacggt
     8581 gccagacgca gtcttcggga aggaaaatat ttttataccg cgcgatcgct ttcatttctt
     8641 tgcggtcttt cggatcgcgg tcgatgagcg actgctcgac atcgaaaccg cgcaggccga
     8701 gcgctgtgtt cgccggaccg ccgcccagaa gaaaccggtc ggctttattc ttgaaatacc
     8761 gtatcacgcc cagcttgtcc gccgccttcg cgccgccgat cacgaagacc atcggatgtt
     8821 tcggcttccg catcgccttg ccgaggaatg ttatttcctt ctcgagctca aggccggcat
     8881 aactcggcaa aaattccgtt atcgcggcga tggatgcgtt cgcgcgatgc gaaacggcga
     8941 acgcgtcatt cacataaaaa tcggcaagcg ccgccagctg ccgcacaaag cgcggatcgt
     9001 ttttttcttc gcctttcagg aaccgcagat tttccaaaag aaaaactgaa ccgcgcggag
     9061 agacggcaac gatttttttt atttccgcga accggaaatg cggcacaaaa atcacttttc
     9121 tattcaacag cagcgtaagt tcccgcgcgt ctttctttag gctcaaaagt ttttcaaaac
     9181 cggatggagc gccgttttta atcgttatat tttccggccg gccgcgatgg ctcactatga
     9241 cgattttccc gctcgtcttc aaaagcagct ttatcgtcgg aatgaccgcc ttcatccgcc
     9301 agtcatcctg cgtattgaaa tcaagccgca gcaacgccgt cccgcgaagc agtttcggat
     9361 ccgccgcgcg taaaaaatga atcatgatag ttcaatgata ccatattc
//
LOCUS       DAIDDT010000029         9157 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_29_length_9157_cov_7.330727, whole genome shotgun sequence.
ACCESSION   DAIDDT010000029 DAIDDT010000000
VERSION     DAIDDT010000029.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9157)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9157
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29_length_9157_cov_7.330727"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2529 a         2110 c         2179 g         2339 t
ORIGIN      
        1 ctttttttat tagtataata aagccagatg aaaaatctgg cgcatatcga attttatttt
       61 cccgaaaaac gcggcgtaaa aaacaagcga gaacttgctg gtttgatatt ggatatgatg
      121 agaaaaagcg gcagtatcgc atatgcggga catcgggacg aaaagagtct gcgtgaaaac
      181 attatgcggc acttgggcga cgcggatgcg gcgcgttata agccattatc ggaatctcaa
      241 aaaaagaaag tcctaagaca aatcgaagcg actatccgca aatgtagcgc cattcttcct
      301 attcccacca agaattacgt tttcgttttt ccttatttgc cgacaaaaaa ggatgcagcg
      361 tttgagggtg tcatgggagt cgccccgtac agttgcgtct ttcacgtctt cctttcgctt
      421 aatatggggt cgttgaaggc cttggcgaag accgtcgccc atgaattgaa tcacacgatt
      481 ttctattacc gccattacga ccatttgaat aactatacgc ttcttgacga aatgatcatt
      541 gaaggactcg cggaaaattt cagggagcag gttgttggcg ggacaccggc tccgtgggcg
      601 gtggtcctca cccgaaaagg agcttttaat gcattggaat ccttggacaa attactttct
      661 tcgaaaaacg ataccgtcca tcgaaacgta ttgtttggga acaaaaagta taaacgttgg
      721 acgggatatt cgataggata ttggatagtg aaagagttga tccgcaaaaa accgaattta
      781 tcttggggcg atattatgaa attaagctcc atggaaatac tgagaattgc tcaaaaataa
      841 agagaagcgc aatgacgatc attgcgcttc tttggcgtgg cagtgcaatt atttatcttt
      901 attgcacttg ccgctacatt tgccggaact gcactgttct tggcatcgga tcgtgccggg
      961 tgcaggcttc tgttccgcag tgactttcca cgagaacttt ttgggaattt tcattgcttt
     1021 cacctccttt ctgtgctcaa gttaaatgct aattaaaatg atgccatagt ttccgtttta
     1081 gcgcaacatt ggcaaggcgg ccagtgcggg tgaatgaatt ggggcgttta aggcgaaagt
     1141 tatcgctttg gcgcgtcaaa aaggggacgc gtccgtcccc agtgggctaa aaggtaaagc
     1201 accacgaatt ctgcatatag tcaaacagct ccgagaatat cggagctgtt tttacgcatc
     1261 ttgaatccat atcccttatc gaaaccttgc aaccacatcg agccacgtgg ggagattcgg
     1321 agccggacgg gaggttctcc gctccaaatg gaaaagagca aaaaagcgat actatcggca
     1381 tgaattgtgt ctaaactcaa ttctctataa cataatgtgc gcctctgcca ttatccgttt
     1441 tctttgcgca tatatgcccg cgcgtaatag gggtatgagg ggctacaaca tcgaccatct
     1501 tttgcgcaag agtatgcgga tgatgatatg gacgggatga ccgcgtggga atggtacgca
     1561 tgcgtcgatt cgatgacgcg cgcttcatcc actgcacgat gcgctttctt acgtttgtat
     1621 tatatcaaaa agtgcaggca ggtcagatta cgctcacata gacgcggccc cacatgatgt
     1681 ggtattgtta acttaatgaa aaataacgtt ttaaaaccat tcgttattat tctcagtgtc
     1741 tttgtcttca cctttggttt tttagcggtc tcgaaaacgc acgcggcgac gctctatgcg
     1801 agtagcacgc tctcaatggc gggcaattgc tattcggtat acccctccct caatattggg
     1861 tacgtgtatg ggaacgattt cgatgtttct gccactttcg gttctgtcgg cgtggcgtat
     1921 aacggcggtt tcctcacagt ccaagttttc tcggatcctg gctatacgtc gcagatcgct
     1981 gcgcggacaa cagcagatct gtacacttct tatcagacgg ggggaacgga catcacccgg
     2041 gacgtggggg ttctgccgtc cggatattac cgcgtcagcc ttggtgtcga atgcagtcct
     2101 tcggcgtcca cgatttcctt cacgggactg actctttctt atgacggaac gtggccgtcg
     2161 ctttctatcg cgtcgctcaa tcaataccaa tccgatgcaa caacaattat cgcggaggga
     2221 tcgataataa ctgaaccaac cgttgttttc gggaccgctc tccaatcgat aaacacgagt
     2281 acgttccagc tgcaggtgga agtgcaaccg gcggggagta atttcaccgg aatcccgacc
     2341 attaccagcc cttttgtatc aagtaacgcg acaacaagct ggacggggcc agacggcaat
     2401 tatcattggc aggcaagaat aatggattct gacgacgcga cgtcaacgtg gaaacaattc
     2461 ggttcgagcg caacctcgac tgactttacg atcaatacgc atccagtgcc ctttaaccaa
     2521 gctggcgatg ccgcttcttc cataggtagc tggtacagcg ataactggta ccagttgggg
     2581 agaggattcg gcggcacgat ccacgatctg attcttgaag cgtcggtgaa ttacggaaat
     2641 tattatcaag aaggtgattc gtatatcacc ctttcagaat ttttcgatcc gaactatacc
     2701 agtatggtcg cctcgacacc attattctct ggttttttgt cgacgataat gagcacgtca
     2761 accaatgaaa ctctcattag tggattgaac attattctca atccgaaccg ttattaccgc
     2821 cttgacactg gctatgaata tcaaaatggc tttgtcgtcc ttcggggaac gactgtattc
     2881 ggcaaggcga tgtacgatca attcatctac ggatcgggac gtgttgaaaa ttactatcag
     2941 ttttatccgt acatcgttgc gaataaaacc gcgccgccca cagtggtaac ttcctcgcta
     3001 gctcaataca aatccgatgc gacgactacg atagcgaaag gcggaacgac gaccgaaata
     3061 ccatcgtcct tgaaggtgtt ccgcaatcat cgtctacgga taaccttaag cttcaattcg
     3121 agctttcgac cagcactgac ccgaacaact ggttttcaaa tccgattatg gccatgagtg
     3181 gaccagttat gtctggacag attgcttcaa tgactattcc caacctgatt gacggctcat
     3241 atcactggca ggcaagagcg atggatactg ttacgaatgc cgcttccgat tggcaggaat
     3301 atggtggaac ggcggggaat gttgattttg tggtgcatac cgtaccgtta ttgacacagg
     3361 ttccatcgaa ttttccctct gtcgttcaaa caagtctttg gtcgcagcag atctatgcaa
     3421 atgggacggg cattctttct cctggtgctt gcggcccaga cattagaagc tgtggttgcg
     3481 ctatcacctc tttcgtaatg aatgctcggt attatggaat catggaagga attgacggac
     3541 aggacataaa tcctggttcg attaattctt ggctcgaagc gacaagcacc ggcaatctaa
     3601 tggccggtta cgattcccat ggcaacgtca aatggcctca agctgcagca tatgcccagg
     3661 atttttccac tggcataggt ttgcattttg acgggttgac gagcggtcaa gccgtgagca
     3721 cgttaaatca atatttgagc caagatgagc caacgatcct ttatgagcgg aacatcggcc
     3781 attatatcgt cgctgacggg caactcgcca cgacctacac gatcaaagat cctgatttct
     3841 acctcacgaa aacattgaat gataatgtcg cgaatactac cacaacacat gattatcaga
     3901 atgcttggac aaacctccgc cttttcagcg caaactttat atcatcgcat atcgcgcagc
     3961 aaggcatcta catgaatctt ggctctcccg ccgaactttt gatcaccgat ccgaacggca
     4021 accaactggg cataaatcca atcacaaata cgacgtataa cggcgtgaat ggcggagtat
     4081 acggttcgga aggcattagc gacggcagcc aagcaaatcc gtccgtgccg acggaaagca
     4141 aatacgcatg gatcccagat ccagtaaacg gcacctacaa tatccaagta atcggtaccg
     4201 gatcggggga ttacaccctt caaactttat cgtacgatgc gagcggtacg gcgcacactg
     4261 cggtagttgt cgggagcacg acgccggata aagagaacgg ctataacttg aatttcacgc
     4321 cgcaacaacc gcagaatatc gccctcgtgc cgcaggatac aacgccaccc gtcatctcgc
     4381 acagcgccgt ttcaccggaa tatctcctca attcttcatc gacccaattc atcttcagcg
     4441 cgaccgatcc cgacagtccg gtagctagta tcgcctcaac gctggacggt accacgatta
     4501 tgtcgccgta tactcttacg tttggtcaaa accaacttgg caaccacgcg attgtcgtca
     4561 ctgcgtccga tcccgccggg aatgccacga gcacgagcgt caactacaac gttaaatatc
     4621 atttcggcgg ttttctgcct cccgtactgg ccaatggatc gggcgtctac aacctcggca
     4681 gaactcttcc cgtcaaattt cagctcaccg acgcgaacgg caactttatt tcgactgccg
     4741 ttgctcgact tgtcgtaacc aatattcaga acggcattac gggctccacg cctatcacca
     4801 tcaccgtttc aacggccaat accggcaatc tttttcgcta cgacagttcc gataatcaat
     4861 atatttataa cttcaataca aagcagctga cttccggcat atggcagatc aaggtcgttt
     4921 tggatgatgg aaatagctat acagtgttgg tttcactgag atgaaaagaa aaactgtctt
     4981 tttcgtgatc gtaggttttt tggttgtagg tgccatggct tattattgga tggcaagacc
     5041 gaaatccttg atttcacttc cggctgcgat tacctcatcg gttgtattaa gtggatcatc
     5101 aactacccaa gtaaatgttg gcagcggggt atgtcttacc agtaatgaag ttgcgagtta
     5161 ttcggccaca agaagtaaag gaaatcctga ttttggtact gttgtaattg atgacaaatc
     5221 aactggcgtg aagatttcta atcttcagat tgaaaacgtg atatcgcaaa attatcatga
     5281 ctatgaatta catccttgtg ggctctatgt cgttaggcaa ttcaacttta atgaaagtag
     5341 ctgggaacct ctccccggat tttcagttga gttatggaaa tatgattata gcgggaaagg
     5401 gaatcgttta tttgttctcg aggctcttca ggggcaatcc accagcacgc tttccatcga
     5461 ttatagttat gatttcaggg taagtccgaa tgaatctttc attgcattag aacgaggata
     5521 tcaaggttct tctgattacg cgttagtaat aaaagatttg aataggttat caaacgtatt
     5581 tgttcttccc gtctctgata ttgcgaaaca gaacccgaat atcatcgctg atctcggctt
     5641 tgaagtcggc ggttggagca ttgatggccg ctatttttgg tttagcttcg cgcaaggtgc
     5701 gaatactacg ggctttgtaa gggtcgacag cagcaactgg gcgtaccaga cattcgctgc
     5761 cccgataaca acgatgggcg gtgacgcctt taatcctgat accggaatgg tgacctatcg
     5821 caccaatgca gcgccttgga ccggcagctc tgaaatcgac caacaatatc gcgaccaggc
     5881 gatgcaatca ggaaaaatca gcttattcta tatttacaat ttactcacaa aacaaagcta
     5941 tcttgtcgca accactaccg acccgacgta ttactatcag ccaaagtgga tctccgattc
     6001 tgtcttgcaa tacacgcccc cttccggggt gacgacgacc tataccatct tgcagtgatc
     6061 atgggtatgc cagtaaagtt caatggaaga aatagtaagt cgtcgatggt tgttcgtgta
     6121 aacgaaagac ggatttacta atctcaaatt ctatgaacca gagcgtcgca gtgggctaaa
     6181 aggtaaagca ccacgaattc tgtatatagt caaacagctc cgagaatatc ggagctgttt
     6241 ttatttgcat aaaaacaaac ataattttgt tttacgttct ttgaactcat acccttgcca
     6301 ttttctctca taggcaccag tttatgcgca taaacatgga cataagggaa accttgcaac
     6361 cacacggggc cacgtgagga gattcggcgc cggatgggga attgtgcgtc ccaaaaggaa
     6421 aatagcaaaa aaatgcggca aaatcggcac gaattgtgtc taaactcaat tttctatggc
     6481 attttcctgc gcatatcatc gctgtttttc ttgcgtatat gcgctcgtca cgcttttcaa
     6541 tataacacgc ggcatatact gatcaatacg gcgccgtcat acgcaaaaaa tcatcataca
     6601 agaggggctg aaatatggtt aaaaacataa cggaatattc atcgaaaaat acgtattttc
     6661 cctatttcat ttcacgctga atagagcgca tttcagaata tgcgcctttt tcgccttttt
     6721 atagccttaa aaaaggcaat aaaatacgcg caataacaag gaaaagctta tataaatggc
     6781 atattcaata atgaacgcgc ataagcatta aaaacggcag aaaaacggta ggaaaaagcc
     6841 aattttctgc atattttgca ataaaaacta tatcgcacaa aaaatgcgct tttcctcaag
     6901 ctttcggtcg attattggcg atcttttccg aggagtgaag tataacttta catctcgtaa
     6961 aagggtgtat catatctcta tgcgcatcat cggacacatc gacatggatg ctttcttcgc
     7021 ttccatagaa gagcgagaca atccgcgtct tgcagggagg cctattgtcg tcggctctga
     7081 tccggaggat ggcaagggac gcggggtggt ctccaccgca aactatgcgg cacgggtcta
     7141 cggtatccgt tccgccctgc cgatatcgac cgcgtggcga ttttccgagg ccgcgcgaaa
     7201 gaaaggattg ccgcctgcgg tattccttga tgtgaacatg cggaaatatg gcgaggtatc
     7261 ggaacgtatt atggagatcg tccggcggca cgtgctgccg gattccaaag gtatcgtcct
     7321 tgtggagcag tcgagcgttg acgaggcata tctcgacctt tcgttcgccg gttcgtacga
     7381 ggaggcggaa cgtgtcgccc gcgaaataaa aaacgacata aaaacaaaag agtggctcac
     7441 ggcatcggta ggcatcggtc cgaacaaact tatcgcgaaa attgcgagcg atcggcaaaa
     7501 accggacgga ttgaccgtgg tgcgcgaaga ggatgccgag gcattccttg aaccgcttgg
     7561 cgtccgcgtg atacccggcg ttggtccgaa gagcgaggcg gcgtttatga aacgaggaat
     7621 acgcaccgtg cgtgacgcaa aaaggatgac caaagccgga cttattgaga tgatgggcaa
     7681 atggggcgcg gagctttatc tgaaactgcg cggtcgtgac gatgcgccga tcgttctcga
     7741 atggataccg aaatccatcg gcgaacagac cacgtttaac gaggatattc ccgtgaatag
     7801 atcgctgaaa gatcggaagg cgcttgaagc cgcgcttgct gcgcttgcga aaaacgtgca
     7861 tcgtcggttg ttgcgcgaga tcgcggacgg catgccgttc acgacattcc gcagcatcgg
     7921 catcaccgtg cgattttctg atttcataac gaagacgcgc atcgtgacac tgaaagaacc
     7981 agtgaaggca tccagccctg tagcattgaa aacaattcaa tttcaagccc tacgactttt
     8041 tatgccattc ctcgaccggc gcgagaatcc gttgatcaaa gcgatccgcc tgcttggcgt
     8101 gaaggtggag cgattcaaat aaaccaaatt atccggtagc ggataatttt tcatccacgc
     8161 atcaaggatg tatattgagc ggatgttttt aaacggatca gcggtttacc tgtccgctgt
     8221 ccaccggatt gcgggaaagg cggtcaagat tccagcagac gacgccgtgc gcttcgctcc
     8281 gccgtatccg gtcgatcatg ccgttgaaga cggtacggcc ggggtgctcg gctgattgct
     8341 tttctataaa ctcttgggta atttctatcc cctctgtttt tgccaaagcc cgcaaaacgg
     8401 caagctggtc gtcaatggat cgcacttgct tgtcttcgac atcggtcgat ttacgggcgt
     8461 agagaaagta ggtcatagtt gtagtatatc acaaccagta aaaaatggcg tataatacaa
     8521 caaagcgata tacttacaga catgagcaac gaagctgaaa agctcggccg gaatctgaag
     8581 cgtattagaa aagaaaaagg cgtatcgcaa ggcgaggttg cgagtgcttt tggaatggat
     8641 aaggcattta taagcaatat cgaaaatggg aagacgaatc cgacgctggc gaccattgca
     8701 aaactcgcaa aagccgtagg cgtaccgatt gaagaattga tgaaataaat ttagccaaaa
     8761 actatggcca agagtaataa atacgatagc tacacgaagg atgagttgat agacgaactc
     8821 gaaactttga agaagaaaaa gtacgggttg gtttgggaca aaaagaactc gcaagagata
     8881 atcgacgctt ttgtgaattg ggagaatgtc ccagaaaatt ttgtaccaaa gcaatttcca
     8941 gttttaaaag aaattaaaaa taaggaaatt gaaacagaca aaacgaaacc agtgaatctt
     9001 ttgattgagg gagataacta tcactctctc gccgtgttga attttactca tcaaaataaa
     9061 attgatgtta tttatattga tccgccgtat aacacaggga atggagatac attcgtatac
     9121 aatgataaga tagtagacaa agaagacgca tatagac
//
LOCUS       DAIDDT010000030         9037 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_30_length_9037_cov_7.019308, whole genome shotgun sequence.
ACCESSION   DAIDDT010000030 DAIDDT010000000
VERSION     DAIDDT010000030.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30_length_9037_cov_7.019308"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    4593..4602
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2317 a         2329 c         2304 g         2077 t
ORIGIN      
        1 aaataatact gacccacggc catactttgt taaactttac ctgcccaata gcatccttca
       61 atcgtcgatc taatttttca tcagtccaat cttttggatt ccaatcagac gaaacgctgt
      121 attcttcttc aatctcatcc cagccttccg ggtccagtaa tctcctagct tcctttttga
      181 acaacgcctc tttttgttct cttgaaagtt ttcctatatc catgaattta ctttatgaaa
      241 tacccaatcg gcttcttgta gagctttgca aaacgctgta gctccacaac atccactcgc
      301 tgttgccctg attcgatgtt cgagatatgc gattgcggcc gccggatttt cttcgccacc
      361 tgcccctgcg tgagccctgc ttccagccgg gctttccgca agcgttcgac gaaataggcg
      421 tattccttgg tctgaacgga cttagtcata tactaaatcc tctatctaat ttgtcaatat
      481 ccaaagtttg gatattgccg cagacagccg acctgccgta taatgaagct attgatgacg
      541 atgtctatga ccccaaaggt cggcatcgtc attttttgta tcaaccatga tcaagcaagc
      601 aaaaggcgag atatggatcg gcctcgcggg tgtgggctac gccatgcgcg ccgaggagat
      661 cgaggagaag ccgaacggat cggtgagctt tgtgcgtctc gtagagggcc gcgacggcaa
      721 gcgcaaggaa cggatcagca caagaaaaga gcatatcatt ttcgtttcgg agaccctcga
      781 aacggagtga tcccctctat ataaatttgc ccctttcggg gagttatcaa cacttacgga
      841 ttggaaactc cttgacgcgc tccggtaaaa tgaagggaac aggcgtgtcg ccaagcagcc
      901 gggcgacccg gacattccct tcattttaat cgggaggacc gctaaggcgc acaaaatgac
      961 catgaatacc atcgccgatt tctatcgccc catccttacc tcgaaatggt tttatctcgc
     1021 catcgccgcg acggtgcttt catacatcgg cctcaacgag tccctgtccg gcttcgcccg
     1081 cgactatgtg cagttcggcc ccgccatcgc ctcgtttcat atccctctgc ttcgtttctt
     1141 tttcctctcc ttccgttatt gcgttcctct cgccgagatc gccattttga gcgatgcgtg
     1201 gtacgcgccc gttgttgagc cgaaatttgc ggataccctt cccgccggaa cgacctggga
     1261 gcagatctgc ctgaagtttc tggatgatga ccgcatcctc atcctcgccg ggcagtaccg
     1321 ccgtgaagtc ggctacgggg agatgggctt caccgacacg cgcggcaggg cgatccggcc
     1381 gaacggacag tggcaattcc tcaaggtgct cgcaaagatg aacggcgaaa ttgccatcgc
     1441 cgatccggag gcgagggacg catacaaaaa gcagaagcag ctcctgtcct catctctcaa
     1501 gaaatatttc cgcatcgatt acgatccctt ccatccgtac cgcgacggga attcataccg
     1561 cattaaaatg acgctcattc cgccggagga agcaaaagcc ggaccggaga tagataaacc
     1621 cgggctcacg gatttcgccg acgatatacg ggaggcgtac gaggagcaaa cccgcctccg
     1681 gtgaataacg cgggctcccc ggtgaaaatc gctttctgaa tatccccgat aaaacggggt
     1741 tttctttttt tctaacccgt ttccggtgaa tttcgccatc aaagggcaaa ggtcgaaaca
     1801 aaaaagacca ccaatgccca tgtacgatga ccctaatttt ttcttcagga catcagacct
     1861 cgctttggcg acggctattt ccgttttggg ggttgcgatc gaagcgatgc agcagacgga
     1921 ttccgagcgg atgaatttca tcttcacaaa atctgacaag ctcaccgaca tcgtgaaccg
     1981 cttttggcgc ggcgaattgc tcgtcgaacc gcaaagctac ttcaaccagc taaaggtctt
     2041 gaaaacgcgg atctaccgcc actaggatgc ttccgcccga ggccatacag gaattcagga
     2101 ttgtttggcg cgaagaattc ggcgaggaga tcgacgacga ttcagcgcgg gagaaggcga
     2161 cgaaccttct ctcgcttttc gccgtgatct ctcaagtcga agctccgccg ggcggcgata
     2221 accatgacga tgcaaccgat gaaaaatttt cttgacgggt acgcggaacg ctttcagaag
     2281 accgtcgaaa catcacagcc tccgatcagg ccgcgcgaca agaaaacgcg gctctggatc
     2341 gacaacgaat atttcgagaa gcgctatgcg gccgtattcc cgcagagcgt ctcactggta
     2401 tacgccgtgt tatgcaggca cgcgaacgcc cggacgcagt cctgctggcc cacagcgcgg
     2461 acgatcatga gcaaggcggg cgtcgcaagc cgcgagacgc tattcgcggc gatcaggacg
     2521 ctcgagacgc accgcatcat cgccgtgcgc cgatccaaag gccgttatcc gaacgtctac
     2581 acacttctcg actgtcgcgc gtggcttccg ccggacggtg ccggaaagcg tactgttcat
     2641 acggaagccg atagtacgat taccggaccg caacagtacg gaagcgaccc gtcgaacagt
     2701 acgaaaaata gcactccaat tcataagatg aaatcagaaa aagaaatcat ggaagatttt
     2761 tctttgaaaa agggaaacgg cgacctcgaa gggagtgcga tgtcggcgct cgatccgatg
     2821 acgcgcagca tcatcctcga ccatttcgaa gtccgcgaag cggaaggggc gttgaaaaaa
     2881 ctggtaaact ccggcagcat attggcaaac ctccgcatca aggaaatcgt ggattatctg
     2941 aggcgttcgg gggtagcaga aaccaagccc attcgctggc tataacacga aaacccatga
     3001 ccatgaacaa tgacacgaaa cccgtccatt acgtcctcta cgcccggaaa tcgtccgaat
     3061 cggaggatcg gcaggtgctt tccatcgact cgcagatcga cgagctgaag gcgctcgccc
     3121 tacggaacga gttcacgata gccgagacac gcttcgaggc gcactcggcg aaagcgccgg
     3181 gccgtccggt gttcaatctg atgctcgacg acatcgagcg cggaaaggtg gacggcatcg
     3241 tcgtctggca tcccgatcgc ctctcgcgca actcgatgga caccggacgg ctgatctatc
     3301 ttttcgatct cggcaagctc cgcgagatcg ttacgcccgg acagatcttc cgcaacacac
     3361 cgaacgataa atttttgctg aaccttcttt gctcgcaagc gaaacttgaa aacgataaca
     3421 agagtatcaa catcaagcgc gggctcaagg cgaaggccga tcgcggtatc tatcccgcgc
     3481 ccgcgccggt cggttacctg aacgatcgcg cgaccgagcg cggcagtcgc atcatacgga
     3541 aagatcccga aaggttcgct ctcgtgcgcc ggatcttcga cacgatgctc gaagggatct
     3601 acgcgccgct ccagatcatg cgtatggcga atcgggattg gggcttcacg atgccgaacg
     3661 gcaagaaact gagccggagc acgatctatg tcatcctcac ccgcccgttc tattgcggac
     3721 gcttcgagta tcccgtgggc agcggaatct ggcataaagg catccatgag ccgatcatca
     3781 cggaggcgga atacgaccgc attcagcgta tgctcggccg ttcgggcaag ccgcgaccgc
     3841 agacgcacta tttcgccttc actgcgatga tcaagtgcgc ggagtgcggc gcgtacatca
     3901 cggccgagga gaaaaccaag catcagaaga acggcaacgt ccaccactac acctactacc
     3961 gctgcacgaa gcggaagggc ttgccgtgct cacaaatgcc gatcacggaa aagaaacttg
     4021 cggatcagat cgaggagaga ttgcgcacga tcgatctgcc taaagggttc aagacgtggg
     4081 gcgtgcgttg gctcgaaaaa acggaccagc agaccaccgg ctacacggcg gaagccctca
     4141 cgaattacga gaaggagctc aagcaatgcg acgagaagat cgacgcgctg atcgacatgc
     4201 gtgcggcgca ggagataacg caggacgaat tcacgcgccg ccgggcggca gcgaccgccg
     4261 agaagcatcg cgtcgaggga ttgatcgccg aaacggaaga tcgaaaaaag aaatggctta
     4321 cgaaagccga tgagattctg gacttcgcga ccgcagcgcc caaaaaattc gccaaaggcg
     4381 atatcgagac cagaaagaag atgctccgca agctcggttc gaacctcctt ctaaacgaca
     4441 ggacgctagt gatttcaatg gaaaaaccgt ttgtgcctct gaaaattatc gccgccgaag
     4501 tgaagtcgat tcacaagcgg ttagaacctc cggaaacatc cgaaaaatct gaggattatg
     4561 agccattgta cgctcaaaat ccagtagtgt tgnnnnnnnn nncttctgtc attgtattaa
     4621 aattactata ccgcaatgct tttgcaagcg cattcagctt tacgaatagt ccggaaattg
     4681 ctaaaattcc ggcgttattt tgccgcggta gctcagtggt agagcgctgc cctgaagagg
     4741 caggcgtcgg gtgttcgatt cacccccgcg gcaccgaatc gaacgggaaa ggggtcgggg
     4801 aaacgggagt ttccccgtgg cggaaatact aaaaccgagg ggttttagag agcgaggagc
     4861 ttcgacgagc gatgtctgat tcttgccccc gcaacaagta aggtttttat tgatcgttct
     4921 atgctacgcg cgaagatatt gcgccgtctg ttaaagtttt ttcgtttcgc tgatttatcg
     4981 tcgtcatttt ttctctgtgt tataatgccg tcatgccaat caaagacgaa ataaaaagtt
     5041 ttttcaaagg cgatgtatcg gatgccgaag cggacaggga aaaatacggc agggattatt
     5101 caatattcaa agttgagccg cagattatcg tttttccgaa ggacattgac gatctgtgcg
     5161 gcctggtcgc gttcgcggcg aagaagaaag cggaagggga ggacgtatcc ctgaccggcc
     5221 gttcggcggg gaccgatatg accggagggc cgttgaacag cgggatcatc gtatctttca
     5281 cgaagtactt caaccatata aaagagataa ccgatcacta tgccgtcgtc gagccggggg
     5341 tgtatttccg cgatctcgaa aaagaactcg ctccgaaagg cctgctctat ccgccttacc
     5401 ccgcctccaa agatttgtgc gctttgggcg gcatggttaa caataattcc ggcggcgaaa
     5461 aaaccctggc atacggcaag accgaagatt acgtccggga actcacgctt gtgatgagtg
     5521 acggaaaaaa acacgtcatt aagccgctgt ccggaaatga actggagctg aaactgaaag
     5581 aacaaagttt cgagggcgaa atctaccgca agctgaaggc gcttatagcg tcgaattacg
     5641 atcttatcaa aaaatccaag ccggacgttt ccaaaaactc ggccggttat tatttgtgga
     5701 acgtatggga caaggaaagc ggtgtattcg atctgaccag gcttatcgtc ggctcgcagg
     5761 gcacgttcgg acttctcgcc gaggcaaaac tgggacttat caaggcaaaa aaatattcgc
     5821 gtctcgcggt tattttcctg aaggatctga cgtgggccgc tgatatcgtg caggagatgc
     5881 tgccgttcaa accggaaagc atcgaatcgt acgacgacaa aacgatcggc gtcgccgtga
     5941 aattctggcg tcagctgatc ggttcaatga aaggcaacgt gtttctgttg gcatggcaat
     6001 ttttaccgga gatgctgatg gtcgtccggg gcggtatgcc gaaaatgatt atgatggtgg
     6061 aaataaccgg cgacgacgaa gcggcgctcg caaaaaaact cgacgatata aaggcccgca
     6121 tcaagcaatt tgccgcggcg catgcgggta tccagattca tctcgtgaaa agcgaaaagg
     6181 aagaggaaaa atactggacg atccggcggc aaagtttttc tatgctgcat gacaatacga
     6241 ccggcttgga cacagcgcct ttcatcgatg atttcatcgt caagccggaa tatatggccg
     6301 agtttctgcc gaaggtcaac actattctgg atcaatataa aaaggatctc atttatacca
     6361 tcgccggcca tccgggcgac ggcaatttcc atatcatccc gcttatgaat ctcaaggatg
     6421 cgcgcgtgcg ggcgctgata ccggtgatat cggatcagat atacaagctg gtgatccagt
     6481 atcacggttc gatcaccgcc gaacacaacg acggcttgat taggagcccg tacctaagga
     6541 tgatgtacgg cgaggatatg gtgaggattt tcggcgaggt aaaaaatatt ttcgatcccg
     6601 agaatatttt caatccgggt aaaaaagtga atgccagtat ggactattcg atgaagcaca
     6661 tcggcggata aaatcgcggc gaacaaaaca atgcatccgt tcgtgtgttc gcccgcgtcc
     6721 cgccgtcgcg gcactatttg tacgtctcga agatcgtcgc gatgccgaag gtcatcggat
     6781 ggacggtcgc gttgctgaag ccgcaatttt cgatggcctg tttgaacgcg ccggcagaag
     6841 ggaatgctcg gatcgattgg ggaagataat tatagggtgt gtccgtatgg aggatcattt
     6901 tcgcgacggc aggcatgatg cggaaactgt aaaaatcata aaacggcgtg agccatttcg
     6961 cctgcgggta agtcaggtcg agacataaaa atctcccgcc cggtttgagc acgcggtacg
     7021 tttcgcggag ggctgccgat gtatccacga catttcttat gccgaaagcc accgtaacga
     7081 catcgaatac gccgtcgtca aaagggagtt tcgcggcgtc ggcaaggaca aaagaaatgc
     7141 ccgccgccgc ggtcttcttt tttgcaatgt ccatcgcttc ctggcaaaaa tcggatccga
     7201 cgactgaccc ctgtgccccg acttttttta caagcagaaa ggcgagtttt ccggtacccg
     7261 tgcaggtgtc gaggactttt tccccgcttt ttatttttgc ctgcgccacc agttttctcc
     7321 gccacagcgc gtccatgccg aaagaaaaaa gcgagcttaa aaaatcgatg gcgggagcta
     7381 tcgatgcgaa catattccgg acggtttcgg tcgttctggg tttttcgcct acgcgcagcg
     7441 atgcccgttc cggttcctgt cttttatttt tagtcatatt ttaaaatatt ccaccgcaat
     7501 gctccgttgc gtaaacgcgg ggaaatttat tttttttaat ttcctttcgc cgacgtgctt
     7561 tttagctcaa tctaatcaat tatggaactt gaaaaaagaa aagtccaatc gattacattg
     7621 acgaacgtgc ggccatttaa gaaaaatacg cgcggatttt tcaaaaaaat gcccgctatt
     7681 gatgaatatc aatagcgggc atgcttatcg ccggacgact ttcgatcgtc cggcatggtc
     7741 aaaaatcttg atttgctttt tcggttacgg cgccggagcg aaaccatttc tgaacaacgt
     7801 ctgcgtcaaa ggtgcttatc ataagcacct cttccctgtt ttccagaagt ttccgcgtcc
     7861 agtcggtaac gccgccgtcg gcgaactgca gccgggtgcc ggcactattc ttaccgaaaa
     7921 cccggaaaca gaaatctgaa taatattcaa tacctgtttt cctgtcgaga tcgaacgcga
     7981 aacgaacctc cggataatcc cgtcgaagtt ccgcaacaag cgtttcatcc agttttccga
     8041 gaaaatcgcg actttccttg atctcgtcaa aaaatgttcc tgtgacgtaa tgcgccattt
     8101 tttcaactat cggcatgaca tcacagtacc catcctcgaa aaagaattga aagtcccgat
     8161 tttgaatatc gcgcatgata tctttttcga tgcggtaatg cgcgataatc ttttcaaaaa
     8221 tccgaatatc ggagaaatga acttcgatat cgccgatgga gaaaccggtt ttatcgagga
     8281 cacgcaaaaa atcaagatat gccctgatgt gtctgcccgc cgcttcgaca agaaatgttt
     8341 cgttgttgtt atcggtgttt cttcccgcag tgcacattcc gaaacgccga aagtgctgtc
     8401 tgaaaaaaac aggcggcgat gattctactt taaacacccg atggctcgtg caaagatcga
     8461 ctcttgtcgg atttttttga ttttttgcga gaagttcttt tctccgtttc gcggcctcaa
     8521 gggcgagtgc caccgcggca tcgctgatta tttcaccgcc atgagatccc gaactaacgt
     8581 cgttttggct tattcccgtc agaacactgt tagttccgag agggaccagc ggagagagtt
     8641 ccactgcttt aaaccgaggt gaaagcgttt ttaagaaaag caggttaaat tcaaggatgt
     8701 cctgctggtc ctctaactca ctcggctgga aatgagaact ttgataatcc tcaagaattt
     8761 cacttggcgc ttgccctgca gttctttttt cgaaaatttc aaggagaaca gcttggaatt
     8821 ctatcaattc aagacgccca aggaaataag gcaagaaatt agttaatcta ttctcgtgat
     8881 aaattttccg ggtgcttgcg ccggtattga tggaaccatt cataaaaacc tccttttccc
     8941 ttttgaggaa gataagatag aagattaaat taagttgtag gttggcatgg cggaatcgcc
     9001 acgcgatact gattgcagaa tactatatct ttatagt
//
LOCUS       DAIDDT010000031         8818 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_31_length_8818_cov_6.012928, whole genome shotgun sequence.
ACCESSION   DAIDDT010000031 DAIDDT010000000
VERSION     DAIDDT010000031.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31_length_8818_cov_6.012928"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2310 a         2024 c         2263 g         2221 t
ORIGIN      
        1 ccttatacgc gcggaacttc ggcgcggcca ttccgggccc gtccacgtcg gcgtgttccg
       61 tgatccatcg caccgcaagg gcgcccgcgt tgtggtaggt ctcttcgagc gccggatcgg
      121 gattccccaa ccctatgatg agtgttttgt ccatgcttga ttattatatt gcatcttcta
      181 tataatgagt acacttacga cattatgaga aagttcttcg cgtcccttct tgaagtattg
      241 gaaatcgccg tcatcgccgt tgcggcggtg ttcattgtgc gaacgttcct tattcagccg
      301 ttcctcgtga gcggcacgag catgtcgccg aatttttcga acggggatta cgtgctggtg
      361 gacgagctta cctaccgcat ccggccgcct gagcggggag aggtcgtcgt tttccacgac
      421 ccgctggact atgggacgta tttcataaaa aggatcatcg ggttgccggg agaatcggtg
      481 cttgtgaacg acaataaggt tactattttc aagaacagtg catcgtgtac cggttcaaac
      541 gggttcgtat tggatgaatc gtacctgccc gcgggaaccg ttacggcgcc ggattcgggt
      601 aatgattgct atacgctttc atcgagcacc tatcttatgt tcggcgataa ccgtccggat
      661 agttttgatt caagaagctg gggggcgctt cccgcgaaga atatcgtcgg gctggtgcgg
      721 gtgcggttgt ggccgctcga cgccattcag gtgttcgccg cgccgaaata ctaattttgc
      781 cgttctcgaa accgttgagg atagccggct tgtagggttc atagagccaa agaaacaaaa
      841 aaagaagaat aagtttttca ccgctcacca tggcttcttt aaaaaataaa aaaagcaagc
      901 agccgcagcg gaagaagttt caggcgccca agggcatgca cgatgtgctt cccgttgacg
      961 cgccgtactg ggaaaaaata gaaacgacga tcaggggatg cgcccgcgag cgcggttttt
     1021 tgcgcatcga gccgcccgtg cttgaatttg cgagccttta ccataaagcg tcgggagaac
     1081 aaagcgatgt ggttgcgaag gagatgtatg tcttaaaaac gaaaggcgac gacgttttcg
     1141 cgctgcgtcc ggaatatacg ccgggagtgt gccgcgcata tctcgagcat aatttgagcc
     1201 ggcacggcca gccgcagaaa ttattttcgt tcggacccgt gttccgccat gaccggccgc
     1261 agctcgggag gtaccggcaa ttcacgcaga ccggattcga tatcatcggc ggattgaacg
     1321 atcccctcta cgacgcagag attatttcca tttatgccga ttttttggat gctttgaaaa
     1381 taaaaaatac cgtgctgagg ctgaattcca tcggctgccg cgtatgccgt cccgcttata
     1441 accgccagct gcaaagttat tataagagcc acgcaaaaga attgtgcgaa gattgcaagc
     1501 gccggctcgc gaccaaccct cttaaactgc ttgattgcaa ggatgcgtcg tgccagaagc
     1561 tcaagggaaa agcgccggat attctgaaca aactgtgcgt gagctgcagc gcgcatttca
     1621 aatcggtcat cgaatatctg gacgaagtcg gcatttctta cgtcatcgat cattatctgg
     1681 tgcgggggct cgattattac aaccggacgg tatttgagat attcgccgac ggaaaggaag
     1741 aagaaatcgg agcgatcgta gcgggcggcc gttacgacta tctcatggaa accatcggcg
     1801 ggcatctgac gcctgcggtg ggcgccgccg ccggcataga gcgcttgatc gccgtgatga
     1861 aagcgaaaga gatcgcgatt ccacgaaagc cgtccaaaaa aatatttctt gctcacgccg
     1921 gcgctccggc caagaagaag gcatttgcat tattgaaaga tctccgcgcg cagggcatta
     1981 cggtcatgga agcgctcacc aaggaatcat tgtcggcgca gctgaagata gccaataagg
     2041 aaaagatcgg cctcgcgctt attctcggac agaaagagat cttcgaaaaa agcgtgattc
     2101 tgcgcgacct tgcggcgggc gcccaggaaa cggttccctt ggaaaaaata gcggcagaga
     2161 taaagaaacg gctgtaggcg atataccaag cgttaccgtg ttgtcatggt cgaatgtcgt
     2221 aatgaaaaat tcaataaaca accctgtcat atgagaacat gagaaccatg ccgatgaacc
     2281 atgattgcct tttttgcaat attgcggcaa aaaaaattcc cgcatacgtc gtttacgagg
     2341 atgcccacgc gctcgcgttt ttggatatca tgccgcggac gccggggcat acggtgataa
     2401 tcccgaaata ccacgcgccg acgttacttg acctgcccga ccgggaattg acgccgcttt
     2461 tcggagccgt aaaagcggtc gacggattgt tatcgcaaaa actcaatcct gacggcatga
     2521 cgatcggcat caatcaggga aaggcgagcg gacaggaagt cggtcattgt catgtgcatt
     2581 tgatgccgcg ctggcacggt gacggcggcg gttcaatgca gtcggccgtt cataatccgc
     2641 cgaaggaaac catggaagca atattgaaga aaatcatcgg gtaaggtgtc tgtagaaaaa
     2701 tatatttaga gttaaaactc attgcgcgtt ctcgactttg tcaggagcgc gtttttgtgc
     2761 gaccattacg ttatgaaaaa agtttcttgt ccgtggtgcg acccttatct gcccagcgga
     2821 catattccgc gatggtggcg catgatcctt tcgcctgtgg accgcatggc gtcgcggatc
     2881 gaccgttttt taaacaatca ttttccacga tttttcgctt tcagcaatgc gaaactgtca
     2941 tcaggttttt ttacagtgct gcttttcacg gggttgttgc gggagcatcg ccttgatgac
     3001 gatcgcgaag atattttcaa tcgcaccatg gtgcttgtgt gcgcggcaca tacgcggggt
     3061 tggccggttt tttcacttga ttttctggga aagccgacga atttcttttc cgtgaaatta
     3121 aaaaacggca aaaaattcat ttttgaagca ctgccgctgg tgccgatcgg cgccgtcctg
     3181 aatattaatt tcgacgataa atctgttttc aaaaagtttt taaaggcgca tggttttcct
     3241 tatgctccgg gcggtctttt ttataccgaa cgcgccgcga tccgttttac ggcgcgctgc
     3301 ggctatccgc ttgttgtaaa accgcacagc ggatcattaa gccagcatgt cgtgtgcgat
     3361 ataaaagacg acgccgcgct gcgccatgct atccgcatcg tgcaaatggt cagttcggaa
     3421 tttattgtag agaggcatat tccgggcgat gtctatcgcg ttacggtggt cggtggacga
     3481 tgcgcctgtt gcctgcgtga aaggccgaat gtgacgggcg acggcatacg gaccattgag
     3541 gcattggtgc gggagaaaaa tgctgcactg tcgcgcgcac cggcgaaaaa aaagaatgca
     3601 acgttgcatg agattaatct tgatgcgcac gcggacgaaa tgcttgcgac gcaaagattg
     3661 tcgcgaataa gcgttttacc tgcgggcaaa aaagcgtact gccatgacaa gcttatcctc
     3721 gcggccggcg ccgacacgta cgatacaacg gacgctatgc atcttgaaaa tcgtgagtta
     3781 ttcctgcggc ttgcaaaaat actcgaagcc cccgtcgtcg gtttcgatgt tatctgccgc
     3841 gatatcgcgc agccgttttc atcgcaaacg tgcgcgatca tcgaagcgaa ctcgctgcct
     3901 tatatcgata tacaccatta tccggcgcac ggcgcgccgc gcgatgtcgc cggatggata
     3961 tgggatgaat gggaaaaagg cgcggcggca ttcatggcgt ggaataaaaa gactccgctg
     4021 acttgaccaa cgcggttcga gtttgctata ctttcatcca ttatggcaga tgatataaca
     4081 ataacggaaa cggaggaaaa taaagcggtt gcatatccga cgggcgaggc ctcgcgcgca
     4141 atgatcgacg cgggcgtttt ttacgggcgc aaaaaaagca agacgaatcc caagatgcgg
     4201 cagttcgttt tcatgaaccg cggcggcatc gacgtcatca atcttcaaaa gacggcggag
     4261 ctgcttgatg aggcgtcgaa attcgtaaaa gaaaaagcgc ggaacaatgg tacgttcctt
     4321 tttgtcggga cggaatcggc cgccgaagcg tccgtgcttg ccattgcgaa ggaattcggg
     4381 atgccgtacg taaccctgcg ctgggtcggc ggcgccatta ccaatttcaa agtcatatca
     4441 aaacgcattg agcatctcaa aaaaacgcgg attgaccttg cgacgggtgc gctcgataag
     4501 tacaccaaaa aagaacggct tgatatggaa aaggaagtga agcggctgga agaacttatg
     4561 ggcggactcg aggaaatgca gcgcgagccg gacgtccttg tcgtcgtcaa tcctgtcgtc
     4621 cataacgcgg cggtgcgcga ggcgaagcgg aaaaatatcc cgattgtcgc gcttgcgaac
     4681 atggatgccg atccggatat catcgattat ctcgtacccg gaaacgataa agcgaaatcg
     4741 agcatcgcgt ggtttttaca gaatatcgaa aaggcgataa aggacggcat cgccatgcgc
     4801 gcggcgccgg ctgcggagaa agcggcaacg caatccgaac ctgcggataa aaattaagac
     4861 aaacaaagag cgaagaatta tatcccgctg ttcgggatat ccggattaaa tccggttttt
     4921 cgcatcggaa tatccaacta tgacgcaaaa taaaaaagaa acatcaaccg aagcggtgca
     4981 gaaattacgg gaagcgaccg gcgccggcgt gatggattgt catcgcgcgc tcaaggacgc
     5041 caacggcgat ttcgacgcgg cagccgcgct tatccgcgag aaaggattgc tcaaaatgga
     5101 aaaacgcgcc ggacgcgaaa cgggcgccgg tcttgtgcgc acatacgtgc ataacgaacg
     5161 catcggcgtc ctgctcgagc ttcgcgcgga aacggatttt gtcgtccgct ccgaaccgtt
     5221 ccagaatctt gcccgcgaac tcaccatgca gattgcggcg gcggcgcccg cggatgcgga
     5281 ggaactttta aaacagccgt acatcaaaga cgaatcgaaa acggtaaaag atctcgtggg
     5341 agaagtcatc gcgaaagtcg gggagaatgt ttccatcgga aaattttccc ggatagaggt
     5401 ataatcagaa tatgttccag tttatcgtta ccgatatctt tatgcttgcc ataggcgctg
     5461 ttttttatct tatggcgcgc gctttgccgc gcattgaaga ggagccggca tcgcaaagga
     5521 aaaatttttt cgaccgattg gcgcattccg acgttcccga aaaagttgat gttgcgctga
     5581 ataatttttt attgaagttc ctgaggcggg tgaaaattct taccctgaag gttgataacg
     5641 ggttaagtaa aatgatgcac aggataaaag cggaagagaa cgccgcgaag cccgctatcg
     5701 attttaagga gatgatggaa cagaacaaag aaaacgatcg gtccgaaaaa taaaccgtgg
     5761 cggacgccgg atcggcaccg tgataattgc atccgttcat attgcaaata tcccggaaat
     5821 cattaaactc aataaggatt aaagcgcaga tggcggagtg gtcaatcgca tcagactgta
     5881 aatctgacgc cttcgggctt cgcaggttcg aatcctgctc tgcgcacttt tgtctacaaa
     5941 aaacaaaagg ctccggcgta agccagagcc tttcggttcc atatttccgc ttttcgacgg
     6001 attagtttat ttgataacca taacagtcga ggcgatatcc cagcccgcaa ggtcgttacc
     6061 gaaattacgc ttcagaatat ccgatgtctt atcaaaatat tcatcgtgcg ctttttgagt
     6121 atcaatgccc tcgggaatct ggtaagtgaa cccgatgccg ttctgatctt tagagacttt
     6181 tctgacgtag agctgaagat aattcggatc ttcagccgcc cactgttctg cgaattttac
     6241 gagatcctct ttgggaatct tggaagaaaa cttccaaaga cccatagccc agtttttttc
     6301 ttgcatagga atggaatgaa attgtgaagg aacggaaagg aatggaacaa ggcaattata
     6361 ccccaaaata ggatattatg caaattttta taaaataagg ctattttgct gatgttgcca
     6421 tcaacatcat tcgaacttcg tcctctcctg cgcacgctgt ttcaaagtag ggtttttcgg
     6481 gatgtttgcg atgcactgat actccggttt ggaaccaatc cgaaattaaa agcattccaa
     6541 tagaaaatgt tgtacattta aaccaactca tcggatatat caaaaaatat acagaggcaa
     6601 aaataagcga tagcgatctc caattagcca tagggaaatt atcctatgcg tgccagacag
     6661 ttgatatttc agccgcagac catataacaa atctccaaga acgccaaccg gccagataag
     6721 atttatcgga agaccgatct tttttaatgt tcaaagatat tatttacgga aagataagcg
     6781 ctaatttcgt tctattctgt gcacggattc gtattttctt cgttgaatgg gcataatgat
     6841 tagatggata atctggatga aatcatttca cagatcagcc tcaaattaat tgaggatgaa
     6901 ggttttgagg gactcgctga tcttgtcgtc caagttaaaa aactgaggga aatagaatga
     6961 atgacgtatt tctgttcgta tacggtacgt tgcgtcgaga tagccgcagc gatatgtatt
     7021 acttgttagc gcggcacgct gactttatag gcgatgcaac gtatcaagga aagctctata
     7081 aaatcgatta ctatcctggc gtcgtgccat cggaggaccc atccgacgtt gtgcagggcg
     7141 aagtgtatcg cctatgcgat tccgattttg tgctgtcacg gttggatcag tacgaagaat
     7201 gcggaccggg gttctcggag ccaacagaat acgttcgtga gattcagtat gttcgtcttc
     7261 gtagtggtaa aaatatctca acatgggtct acctttacaa tctacccacg aacgaccttg
     7321 cgttggtacg atcggggcga ttttcttaat accagagccg ctggcattat tcaagagctt
     7381 tccgttcccg tgtatggata aaaaatcccc gtctatgggg attttttatc catctttaga
     7441 agctatgaag tacattcaca agaatcgcat ttcttgcctt cgtgggcgtc atgatgacat
     7501 ttgtggcagt ggcaagtaca ttcattatgg tcgtgatcgt gttcgacgtt acattttgga
     7561 caaatcattt ttagatatat ttttagatat aatttatatt atatggcgac ctttttgagt
     7621 gctgaatcat ttcattatat catatagcag atgttttatt taattccctc ggccctgttt
     7681 gaaaatcgtc cacgcccgcg cacggttcat ccggcggtat gcttttgcac gattactgat
     7741 gatatgatgg gatatatcga ggagtaatac ggcacgccgt cttttatgcg gcagttactc
     7801 tgttttttat aaaaaaagag cctccggcgt tgcgaaaaaa tcgcgccaaa ggctcaagag
     7861 ggatccggtg ggtaccatca attttcagaa ctttctgatc gcaccgacgc ccgtgtagtc
     7921 attgatgtta tcccattggg gacggacaac gacgccgccg gaaacgcagt agccgacgcg
     7981 gataatacga ttattctcgt cgaagcttgc cgtgttggca tagctgttca gattcgcata
     8041 gactcctatt tttttataca acaggatgtc tatcgcgcat tgctcgactg ccgtcggaac
     8101 tttataccct ggcggcaaca tcgccttctg ctcagcgaac gttttattct ccgagcctgg
     8161 tatgacgcca ataagggaaa gataccaacg taatgcgagc gtcgttttgg tggcaaactt
     8221 ctggagtttg aaccaagtca gtggtacata agagtggaaa cagggtttct tttctttcgg
     8281 attaaacttc cgaagttcca ttatagtgat ggtgtccagg cccgcgaaca ggaaatgcgt
     8341 atcaccgaca atcttaccct tgtagaacgg gcacggcgat ttaaggattt tatcgggcaa
     8401 agaaaaattg ccgatagttt ttacctgttt tgacgttgta aaaattccgc atttttgcca
     8461 gtcggcgatg tcaaagtatg gcatcgccgg aagatcggga ttgttcgtta cattcatttt
     8521 cttttctcct ttttaaaatt atcggatgtg ttttctgatt gagacgttcc tcgcatcgat
     8581 aaaaaccctg cattttttca gttgtcaaag aacggataca ttataacaat gtatgacaat
     8641 gacgtcaaga cgccggaatt tggtttaatt ggtacatgct tctcatgata gcgaagttgt
     8701 accaacacgt tcatattatt ccagggatgg caatggaaaa ttgaatacac ccaacaatgt
     8761 ttgacattta caatatgaca ttttatgctt tcatcagtaa tcaagccatg gcggcttg
//
LOCUS       DAIDDT010000032         8813 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_32_length_8813_cov_4.816277, whole genome shotgun sequence.
ACCESSION   DAIDDT010000032 DAIDDT010000000
VERSION     DAIDDT010000032.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8813)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8813
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32_length_8813_cov_4.816277"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2735 a         1878 c         2352 g         1848 t
ORIGIN      
        1 tatggaaaca gccgtttata atttaaagaa cgaaaaagta ggtacggtgg aattgccgga
       61 ccgtgttttc ggcgcgaaat ggaatgcggc gctcgtgaaa caggtgtttg acgcccagcg
      121 cgcgaatgcc cggagtccgt gggcgcatac gaaaacccgc ggcgaggtga gcggcggcgg
      181 caaaaagccg tggcggcaaa agggaacggg acgcgcacgg cacggttcca cacggtcgcc
      241 gatctgggtc ggcggcggag tatcgcacgg cccgcgcaac gacaaggatt attccgaaaa
      301 agtgaacaag aagatgaagc ggctcgccgt ctttgcggtt ctttcgaaga aaatgaagga
      361 aggcgaaata aaagttttcg agaattttgc gattgaagcg ccgaagacaa gggtactcgc
      421 gccggcattg cggaatattc tggggatgag aaaggacgca aaaaaattcg acgtgcttct
      481 ggtcgcgaat accggcaacg agaacctttt ccgcgcttcg gcgaacctca cgaaagcgaa
      541 agcgttgaac gccgactcgc tcaatatcta cgacatcatg aaccacaaga attttttcat
      601 ggaaaaagga gcggtggcaa cgatcgaaaa gcattacaaa gtttaaaacg cgccactatt
      661 ccaaggattg aataatttat aacatcatga ttgattcttt tatcataaaa aaaccgtggg
      721 tgacggaacg ggcgaccgat ctcggcgcgc tgggccagta tgtttttatc gtgaagccgc
      781 atgcgacgaa gccggagatc aggaaagcga tccatgcaat ctataaagtg gatgttgttt
      841 ccgtgaatgt gacgaatcgt ccggcgaagc ggaagcgttt cggcggcatc atgaagggga
      901 aaaaggaagg atatcggaaa gcgatcgtaa cattaaagga agggcaaaaa atagagatac
      961 aataattaaa cgaaaagtga aaaactaaaa acgaaaaacg caggtattcg ctccgcgcat
     1021 tcattcactt tttgctattc actattaact tttcacttta ttccatgaaa tcctataaac
     1081 ctaccactcc gtcgcggcgg catatgacga cctcgaatta ccggggactt accaaagtcg
     1141 ctccgtggaa gccgcttttg gcaaaggttt cttcccgcgc cggaaggaat tcgcagggac
     1201 gcatcacggt gcgccatcaa ggaggcggca ataaaaaacg ctatcgcatg atcgacttca
     1261 agcagaaccg tatggaaaaa gcgatcattg aaacattgga atacgatccg taccgcacgg
     1321 cgttcatcgc gctcataaaa tatcccgacg ggacgcggag ctatattctc ggatgggaag
     1381 ggcttgcgac gggtacggta atcgaaacgg gggagtccgc gccgcttgcg gcaggcaatc
     1441 ggatgaagct caagaatatt ccggtgggtt atcgtgtttt taacgtggaa atgattcccg
     1501 gacatggcgg ccagctcgcg cgttccgcgg gatcgtacgt tgaagtgctt gccaatgccg
     1561 acaattatac cgatctgaaa atgtcgtcgg gcgaagtgcg ccgcgttccg tgcgagtgtc
     1621 ttgcttcgtt ggggcaggtt tcaaatgcgg aatggaattt gatcacgatc gggaaagcgg
     1681 gccgttcacg atggcttggc atccggccga cggtgcgcgg taaagcaatg aatcccgtcg
     1741 accatccgta cggcggcggc gaaggaagcc agccgcgcgg caccagaaag ccgaaagaca
     1801 tctggggcaa tattacggga ggccatagga cgcgcgataa gaagaagcgg tcgggcaggc
     1861 ttatcataaa aagacggaaa taaattaaac gaaaagcgaa aaacgaaaaa ctaaaaatga
     1921 agatattcgc tccgcgcatt cattcacttt tcacttttca ctattcactt taaacttttc
     1981 acttattatc atgtcacgat cacttaaaaa aggcccctac gtcgatccga agcttctttt
     2041 gaagattgca aaaataaagc ccgacagcgg cgaggttatc aagacgtggt cgcgcgcttc
     2101 ggaaatcgct ccggagatgg tgggatatac gttcggggtc cataacggga agatattcat
     2161 agaagtgaag ataagcgaag agatggtcgg ccaccggctc ggagaatttt ctttgacgag
     2221 aaagttcgtc cgccacggcg gtaagattca gaaggatctc gagatgaaga cggcggccgc
     2281 gcaaaaaacg gcggcagtgg cggcaaagaa aaaatgacga gaatggtgtc gcgaaaaata
     2341 taacatcgga aacgccgcga cgctgaaata ttacaatatt acaaatcatg gaaaagcaaa
     2401 ccgcacaatt gaattatctg aagattgcgc cgcgcaaagt gcggagcgtt ggcgatctca
     2461 tcaagggact taccgtgaac gaagccgagg ctcagcttct ggtgcagcgc cggcgcccgg
     2521 ctaaatcgct cctgaagctc ttgcgttccg cggtcgctaa tgctaaaaac aacaagcggc
     2581 tgaatgttga ccatcttttc atcgagagta tccgcgtgga caaaggaccg atgttgaaaa
     2641 ggtctttacc ccgcgcgcgc ggcagtgctt cgcccatcca taaagtgatg agccatgtga
     2701 cgcttgtgct tggcgtgaat gaaaatctga agccgcggtt taccattgtg gttccgaaaa
     2761 aagcgaaacc ggtacccggt caggaatcat ataaaggaag caggtcaaaa agagcgccgt
     2821 ccgtcaaaaa aggaaccgga acattggcgg aaaaggaaaa cgtcgagatg aaaaaatcgg
     2881 ggtttttgaa aaaagtgttc aacccgagcg cgcggcaaaa cagaggagat aaagtttaat
     2941 gaatagagaa tttagaattt agaatggagt aggaatacag aattccgagt ccgaccacta
     3001 aattctttat tctcaatgcc actaaagaca tgggacaaaa aataaatcca aattcattca
     3061 gattgggaat cgtccggaac tggccggtgc gctggttctt gaagggcggc tacgggaaat
     3121 atctcgaaga ggatgaagcg atccggaagg tcgtcaacaa aaagatcggc gaggccggca
     3181 tcgccgcgat agagatcgag cgcacgcagg ggagtttgcg cgtgttcatc aaggcggccc
     3241 gtccgggatt cgtcatcggc cgcggcggca agggaatcga agagctcaat atggcggtcg
     3301 cgaacgcgtt gcgcaaagtg aggaaaaaca aagcgccggt caatctgaat ctgaacgtgg
     3361 aagagctcaa gcgcacggaa gtttccgcgc agtatgtcgc ccagcagatc gcgtgggacc
     3421 ttgaaaaaag gatgaagttc cgccggacga tgaaaaaata tctggagcag gtccagcaga
     3481 gcagggacgt gaaaggcgtg aatgtatttc tttccgggcg gcttgacggc aacgagatcg
     3541 cgcggcggga atggcttgcc aagggttcca tcccgcttca aacgctccgc gccgacatcg
     3601 attacggcac ggcgacgtcg cgtacgaccg ccggtgctat cgggataaaa gtctggattt
     3661 ataaaggaga gatattcgcg aacgaagaaa aaaagaaaaa cgaggaaaaa ttacaaagag
     3721 aaagacgagg atagaaaagt tataatgttg taatgaaaaa tgcggcatga gaacgcctga
     3781 acattacaac gctacaacaa tcaatatgct tattcccaaa aaagtaaaac atagaaagtg
     3841 gcagaaagga cgctcaaaaa agcggaaggt tgaaacacgc ggcattgagt tgaattacgg
     3901 ccagttcggc ctcaaagcca tggggtcttc gtggctgacc tcgcggcaga tcgaggcagg
     3961 gagaaagacc atcacgaact ttctcaaaag gagcggccgc ctttggatac gcgtttttcc
     4021 ggacaagccg atcacgctgc aggcaccaga aaaaactttg ggcggcggca aaggatcggt
     4081 cgaccattat gtctttcccg taaggcccgg caggattatt tttgagattg acggcgtgcc
     4141 cgccgaaacg gcaaaagagg cgttgcgcct cgcatcgttc aagcttcctt ttaaatgcaa
     4201 agtaatcagt aaagaacaga gataagaaaa ttaaaatatt tttttaatcg ctgacgattt
     4261 ttatcatgaa gaaaaaagaa ctgcaagaat taaaaagcaa aacggccggt gatctcgcgc
     4321 atcttgcgcg cgaaggatct gagaaattac gggcgcttaa atttgacttg tcggccggaa
     4381 gggtgaagaa tgtgaacgag attcgcgcgc tcaggaagaa tgtggcgcgg atgcagacat
     4441 ttataaaaca aacgaaaaac gaaaagtgaa aaactaagaa tgcagatatt cgctttgcga
     4501 attcattcac ttttcaccaa tcacttttaa ctattgactt attatcatga ccgcatcaac
     4561 gaaccaaaag aaaataagga agatcacggg cgtcgtcgtt tccgataaga tggcgaaaac
     4621 gcgcgtgatt gcggttacgc gttcaaaaaa gcatcccaag tacctcaaat attacgaggt
     4681 agtgaaaaaa ttcaaggcgc atgacgagaa caacgaatac aaagccggcg acaaagtggt
     4741 tatcgaggaa acaaggcctc tgtcgaggga gaagaggtgg aaaataatac aaaagatata
     4801 aaggttgtaa tgccgtaggg ttataatgtt cccgtgccgc attttccatt acaactgcaa
     4861 cattaaaaca ttccaacgtt ataacaatca atcatcatga tacaggaacg atccatttta
     4921 aaagttgcgg acaacagcgg cgcgaaaatc gtgcggtgtt ttcggatact cgggggtaca
     4981 cggcggcggt atgcgggcgt gggtgatatc gtggtcgctt cggtgcagac cgcggaaccc
     5041 cggaaggcag tgaagaaaaa agacatcgtt aaggttctca tcgtgcgtac caaaaacgct
     5101 ctgcgccgca aggacggttc gtatgtccgg ttcgacgaga acgcggccgt ggtggttgat
     5161 gctaaaaaag aaccggttgc gacccgtatt ttcggaccgc ttccccgcga gctcaaggaa
     5221 aaaggatacg aaaaactgat gactatggcg gaagaaatcg tatgaaaaag ttaaagagtt
     5281 atagtgttgt aatgttctat cgatcgagga cggtctcgtt atagtgagca ttttcattac
     5341 accattgaga acattacacc attaaaacat tacgatcatg aacttaaaaa aaggagacgc
     5401 catcaaaata atttctggaa aagaccgtgg caagacgggg gctgttttta cggtttttcc
     5461 cgaaacgggg aagattgccg cggacgggct taatatgttc aaaaagcgat ccaaaccgaa
     5521 aaaacaggga gaaaaagggc agatggttct tgttccgcgt ccgtttccgg cttcaaaagc
     5581 gatgcttgtg tgcaaaaact gcaagcagcc ggcgcgggtc ggccatcgca tggagggcga
     5641 taaaaaagtc cgttactgca agaaatgcca ggccaatacg taaaatagaa ttcagaattc
     5701 agaatagaga atgaatacaa aagaacaatc taaattccaa attccgttct ctgttctcaa
     5761 tgctaaattc tatattctca atgctaatta attaagatat ggcaaacaat caaaaacaac
     5821 agaataatca gagggcagaa atccgcgcgt tcttggagaa ggtcgtcgtg gatgccggtg
     5881 tcgggcgtct tggccagcag ccgaacttcg aagaaaaagc tttgccgcag atcgtccgcg
     5941 acattgccgc ggtcaccggg cagaagccgc agatccgccg cgcgaaaaaa tccatcgccg
     6001 ggtttaaatt gcgggaaggc cagatcgtcg gactccgtgt caccttgcgg aaggcgaaaa
     6061 tggttgattt tttcgagcgt ttgattacaa tagtattgcc tcgcgtgagg gacttcggcg
     6121 gcttggatct aaaaacggtg gataacggcg gcgttctcgg cgtgggaata cgggagcatg
     6181 cggtgtttcc cgagatctct cccgaacagt cgccggtttc gttttctatc ggtgtgagca
     6241 tcgtgccgcg ggcgaaggac cgcgaacggt cattggaaaa attcaggcaa tgcggcgtcc
     6301 cgttcaaaaa agaaaaaaag cgtaaatagt tttcaatgtt tgaatgttct caacggtgta
     6361 atgaaaatac aaccatagaa cacaaaaatt acaacattcc aaccattcat taatcatggc
     6421 aaaagaatca gtcatcgcgc gggctaaaaa gcgtccgaaa tattccagtc gcatcgtgcg
     6481 gcgctgtttc cgttgcggca ggaagcacgg ctatatgagg gacttcaatc tttgccggat
     6541 atgtttccgc gaaatggcga ccaatcagca gattccggga ataaaaaaag catcatggta
     6601 aaaaaataaa cgaaaaatta aaagtgaaaa acgaaaaacg caggcattca cttttcgaat
     6661 tcacaaactt ttcactattc acttttaact tttcacttaa ttatgtacta cgatcttctg
     6721 gcaagaatta aaaacgccac catggcaaaa aaggaaaaga tgacggtgcc tttttcgaaa
     6781 atggatgccg ctgtcttgaa agcgcttgtt gaaaacggct atatcaaaag cgccgatgcg
     6841 gagatcatcg ggaaagaaag cgttatcgtc gtgcgccttg cctataaaaa taaagcgggc
     6901 tccgtgagcg attttaaaat cgtaagcaag ccgagccggc gtttttattt cgattatcgg
     6961 agcttgcgtc cggtaaagca gggctatggc gttgccgtcc tttcgacgtc gaaaggtatt
     7021 atgacgaaca aagaggcaag aaaacaaaaa atcggcgggg aatatttatt tgaaatatgg
     7081 taaataatag aatatagaat ataggagaca ggagacagaa tgagaatcaa tccacgtttt
     7141 ctattctcta tgctaacttc taatttctaa attctaatta agacaatgag ccgtttaatc
     7201 aaaaaaccga tcataataga aaagggcgcg accgttacgg aaaacggcgg cgttttgaat
     7261 tttaagggac caaagggcga gcttaccgtg gcgttggttt ccggtatcgg cgcgaagatc
     7321 gaaggagaaa atctctggat agaaaaaaag ggggacgaag cgccaaaggc gatcgccggc
     7381 accttatggg cgctttcaag gaatgccatg gaaggcgtga tgaaaggatt cgtgaaagtg
     7441 ctcgaaattg agggcgtcgg ataccgcgcg gtgcttgagg gaaaagacct tattttacac
     7501 ctcggatacg cgttaccggt ccgcgttaag atagcggacg gcgtcacgct tgccgtcgaa
     7561 aagaacgtca taaaagtttc ggggatcgat aagaatcttg tcggtcttgt cgcggcgaag
     7621 atccgcgcgc tgaagaagcc ggagccgtac aaagggaaag gcatccgtta tcagggagag
     7681 gttatccgga gaaaggttgg caagaaagcg ggaacagccg cgtaacgcgt aaaaagaata
     7741 aataggatag agaaaacgca gaagatagag aagataaaag aatccatcta aatcctatat
     7801 tctcaatatt aaattttaat taaaacatga atccaagaaa aaaactcaac caggtgagaa
     7861 tgcggcgcgc gcgaaagacg aaagcgaaga ttttcggtac cgccgcaatg ccgcgacttg
     7921 cggtttatcg cagcaaccgt tccatcgccg tccagcttat cgatgaccgc gcgggacgca
     7981 ccttgatttc ggcatcgagc aaaacgcttc caaaatcgcc ggaaaaaaag accaaaagca
     8041 cggcggcttt ttccgtcgga gagcttgcgg gaaaaaaagc ccttgaaaag ggtatcacaa
     8101 aagtagtttt cgatcgccgg tcttataagt ttcatggcag ggtaaaaaac atcgccgaag
     8161 gcgcaaaaaa atcaggactc gtcatttagt aatatcaaat aatctaattc atcatgttca
     8221 aaagaggaga taggccaatt aaaaaggaag agtttaagga aaaagtgctt gatatgcgcc
     8281 gcgtgacgcg cgtcgtcgcg ggcgggaaaa gattccgttt ccgcgcgacg atcgttctgg
     8341 gcgatgaaaa aggaagggtc ggcatcggta taggaaaagg cctcgacgtc cagcaggcgg
     8401 tcggaaaagc gaaggcggac gcgaagaaaa atatgctcac ggtcaatttg cgggaaggcc
     8461 ggactatcgc gcatgaagtc gatgcgaagt acagcgcggc gaaggttctc gtgaagccgg
     8521 cgagccgcgg ccatggattg cgatgcggcg gttccgtgcg tttcgtgctt gcgttcgccg
     8581 gcatcaaaga cgcgacggga aaatgtctcg gcaggacgcc gaataaactg accaatgcca
     8641 tggcaactat cgaggcattg aagaaactga aagcgtaaat aaaaaataga atatagaaat
     8701 cagaatttag aatagagcat acagaagata aaaaaataac caatctaaat ttaaaatttt
     8761 gctttctatt ctgatttcta aattctaatt aagacatgaa actacattct ctc
//
LOCUS       DAIDDT010000033         7859 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_33_length_7859_cov_6.752249, whole genome shotgun sequence.
ACCESSION   DAIDDT010000033 DAIDDT010000000
VERSION     DAIDDT010000033.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33_length_7859_cov_6.752249"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1889 a         1932 c         2117 g         1921 t
ORIGIN      
        1 caagagaatc gatacttgat tataagcaac aagaggttat ctttcataat ttttaccatt
       61 tttgttcttt ctattgtcat cgtccggtat tatggcatag aatgggttcg gacgaggatt
      121 ttgctgttct tgatttcggt cgacgggatc agggcgggtc gcgaggacga cggagcggga
      181 gtttcattta aaacaatttt cattctatcc atatccatga tctcattctc cgttcttaaa
      241 aattcaaagc gcagtaaggc gcggctcggc gttttgaaga cgccgcacgg tgaaatcatg
      301 acgcctgcct tcgtgccggt cgcgacgaac gccgccgtga aggcgttacg cagcgacgag
      361 attgcggcga ccggaacgca aattcttatt tcaaacacct accatctcca tctcatgccc
      421 ggcgagaaaa ttgtaaaaaa agccggcggc atccactcgt tcatgaactg gcaaaagccg
      481 ctcatgaccg attccggcgg gtttcaggtt ttcagtctgg gattcggcag ggatctcggc
      541 gtcggcaaag tcacgaaaca taaattgtat ccggcgaagc tcgagcgcgc cgtggagatt
      601 ggacaccagc cgaaatcatt gaaaataacg gacgacggcg tgcatttccg gtcgccgctc
      661 gacggcaaag aaatttttct cggtccccgc gaatcaatcg ccattcagga aaagcttggc
      721 gccgacatta tgtttgcgtt cgacgaatgc acgccgccga aagtaacgcg tgaatatatg
      781 aaagtgtcgc tcgcgcgcac gaacgtatgg gcgaagcaat gcctcgccgc gaaaaaaacg
      841 gagcaggcgc tttacggcat cgtgcaggga tcggcatatg aggatttgcg gaaggaaagc
      901 gcccgcgcca taaacgccat ggacggtttc gatggcttcg gcatcggcgg cgacctcggc
      961 gatgccgtgc agcatccttc ggcggcaaag acgggaaccc ggaccatcct tgcatggacg
     1021 ttgccgctgc ttgacgcgaa aaaaccgcgg catcttttgg gtatcgggca tatcgaagat
     1081 atcgaaacta tcatccgcgc cggcgtcgac acgttcgatt gcacggcgcc gacgcattac
     1141 gcgcgccgcg gcgtcgcgtt cacgagtcaa ggccgtcttg cgatgcaccg cgcggcgctg
     1201 ctcgacgaca aaaaaccgct cgacccgaaa tgcggctgca tggtatgcaa aaattaccgc
     1261 aggcattaca tcgcgcatct catccgcgcg aacgagatca cgggcggcgc attgctcacg
     1321 ttccacaatc tttatttctt caacgcgtac gtcgaaaaaa taagggaaaa gatcaaaaac
     1381 gggaaatttt gagattgact cactttccgc cgtctatcga cgcgcttact ctttatttct
     1441 gtcgtcctcg ctttcgcgag gacgacagaa attcttttcg ggagggcgac agaggggaag
     1501 gcgatggttt ggcttatcca cagttttcat ggcacttgct ttattgaata tgctataata
     1561 aaaaacaatg aagcagttta cccgacagcc tcaacccgaa aattataaaa accgctcgcg
     1621 agagcgcaac ctccgttatc ggacattgat ttttatcgcg gcgttttttg ccgtagcatt
     1681 cggcgtgtca ttgggcattt tccggtcgct cgctttcacg ggtcccacgt cgccggccgg
     1741 tgtcggttcc ggcgcgattg ccacggacgc ctcttataat atcgcgatcg gcacttcgac
     1801 cacgcaagcc ggtaccaagc tcctcgttgt cggggcgacc tcagacaaca ctacctacgg
     1861 tttccaggta ttgaacagtg cccagacacc tcttttcacc gtccgcgacg acggacaggt
     1921 ctctatccca aatcttaatg tatcgtcggg aactttcagc ggcgtcttta ctgggaccat
     1981 caccgctggc aacgtctctt ccggtcagtt cggttccaac accggcggag gcaattattc
     2041 ttttcccgcg gacgttggga ttgggacgac ggcaccagcc agaatattag atattaacaa
     2101 cgggttgact agcgcaggaa atggctcggt gagggtgtac ggctatcaag cgggtttcga
     2161 ggtttacaac gcagccggaa ctgctaattg gtattttgca aataatgatg ccgacagcaa
     2221 cagtctatat attggaagag gttatggacc aaatcaaggg atagcgccga atattactgt
     2281 gacgcaagcc ggcaacgttg gcatcgggac gacgagcccg ggggctccac tgcaaattaa
     2341 tcaaactaac tcgtccgtcg ccttgggcag tgccgctctg cgtatagaaa atccttcagg
     2401 atcgcagtcg atgctttatt tcagcatggc aggcgcccag aaggcatcga ttcgtgctga
     2461 ttcctcgggt aatctagtat taaatgctgt tagtggcaat atttttctca accatgattt
     2521 tggtggagag ccaatcattt actttcctag tgccattgat tttgccggcg gcaacgttgg
     2581 catcggcacg acggcacctg gtaacgtgtt agatgtgcaa gggggcaaca tagatgcttc
     2641 tggatatctt atggcgggtg gaacaaacgg cggagctgtt atgttcagcg ggcagactcc
     2701 ggtaaacggg aacggccccg gcggtatatc tttctatggt cgtcctcgaa ttggcgattg
     2761 ggatcaatgg ttgacgcttg gcgaaccaaa tgggacgaat gggggaatga tccttcaaat
     2821 tcaaggatcg gcaatatata caggaatttc ttctggcata accgttgcgc ccactcttca
     2881 caatacttta gataatggtt ccggcgatgt tggcatcggg acgacgagtc cgggatatcc
     2941 attgcaagta aatggctcaa ttgacgcaaa ctctccacca agcaattctg tggatatagg
     3001 tacgtccgcg gcggccaacg atacctatca gttaatagga acaggtgggt attggggaat
     3061 acggcaaggt acttcaaata attttaatat cgatatgtat aatagtgggt cgccaataaa
     3121 tgccttaact attacctcta atggcgaagt cggcatcggc acgacggcac ccgcctatcc
     3181 attggatgtt tctggtgtag gtcgttttac gagttccgtc gattcgtcat gggttggatc
     3241 ggttacagga aacaatggtg gaacaaatgg aatccttggt ggacaggtca ataatactta
     3301 ttggacaggc gttgcaggag aatcgagtgc gggtaacggc attggcgtct atggatcagc
     3361 ttcttcggtc ggcgttggcg gatattttat aaataccgat ggcggcacag gtctggtcgc
     3421 ttacagtacc gttgccggcg gttatggcat atccgccgta ggtagctggg aaggcgtaca
     3481 aggcgaaggt aaaaccggtg tgtatggtat aggcatcggt gccaattcct tcggcgtata
     3541 tggcatgacc aacggcaacg tggggataga tttcataggc gggaacggcg agtatacttc
     3601 cggtggcaca tgggtgaatg cttccagtcg cgcgctcaaa gaaaatttca cgcccgtcaa
     3661 tgatcagtcg atcctcagta agatcgtcgc cttgccgatc acgcaatgga actataaatc
     3721 ggacacgaag gcgctacata tcggtcccat ggcggaagat ttttacgcca ttttcggatt
     3781 gggcgataac aataagagca tttcaaccat cgatcctgcc agcatagccc ttgtcggcgt
     3841 caaggcattg gatgaaaaga tcaatacgca acaacaggag attgatacgc tcaaaacgca
     3901 gaatgctacg cttgtaaacg aggtgcaaca actgttaaac aaataaagac aacaaaatga
     3961 aaaaaacaac aattatcgtt ggtattatta ttgcgtttgt cgcaggttta gttggcggat
     4021 attttggggg atatgccgaa ggcggcgcgg cagttacggc attgtatgct tcgaagattg
     4081 ccgcggtcgg cagatttttt ccgatgcctt cgcaagtatt ttctttgagc gggcgcgttg
     4141 aaaatataag cggtgacacc attacgattg atgcgaactc cataacgcag aatccgttcg
     4201 ccgaagggaa ttttcccgca gtccgcacgg tggcggttac cggagcgacg gtaataactc
     4261 ggatggaaca aactgatttt gcgacgcttc aaaaagagat ggcggcgcag agattgtccg
     4321 catcaacggg aaccgtcggc gcgccgatca ctccgccttc gctgttcacc gaaacggaca
     4381 tagtattatc cgacataaaa gtaggcgata tgataatggt aaccgcggca agtgatatag
     4441 caaacgccgc gagtttttcg gcgaccaaga tccagttgtt gccagaaaac tccgctaacg
     4501 ttccttttcc ttcttcttcc gttcccgctg ctacccaaac tccagcacgg taaattaatt
     4561 aaaaaaggaa tgacagcgtc agatatccct ttacaataaa agggtatctg acgctgtttc
     4621 gtgtcctgtt tttttaaatt cttttaatct ttatgtggta tgctgtgttc tatgaggacg
     4681 gcgagattct cggcgtccaa gaaatgatgt ctgacgctgt tatcataaag acatgaccaa
     4741 gaaaatgaaa aacgataaaa aagggttgtc tgtttcagag cgccatttcg gtgtaatttt
     4801 ggaagatatc gatgccaagc tctatttggt gttagagggg catcaatctc tggatataaa
     4861 aatagataaa aaccatcagg agtttcagga attccgcgaa gaggtgaact ataagtttga
     4921 cgttgttttt gaaaaattcg atgaaattga tatacgtttt aaaaacattg atatacggtt
     4981 cgacgccatt gatatacggt ttaaagatgt ggacgcacgg ttcgataacg tggaagctga
     5041 gtttaaaaac gtaaaagatg aattacgtgt tattcacgac gaactcaagg aaaaacccgg
     5101 ccgtgatgaa tttactcttt tagaaaagcg gacaatggct ttggaaaaat caagaaaata
     5161 aaaaactctt tcgggaggca ggacaatagc gtcggcttcg acgttattat tcccaatgga
     5221 tggatgcttc gaatgcggcg aacttttctt tgaggagcgg cgcgcgcagg agatacgggt
     5281 cttttttgag gatgtccgct gccgcttcgc gggattgttt cacgagcagc gggttgcgga
     5341 cggcttccat cgcggcatcg gaaaatccag attgcgcgac gccgaaaaat tctcccggtc
     5401 cgcgcagccg caagtcggct tccgcgagct cgaatccgtt tttcgcgctt acgatcgctt
     5461 ttgcccgcgc gcccgccgcc gcgcttccgg cgtcggccat cagaaaacaa tatgaccggt
     5521 gcgcgccgcg tccgacgcgc ccgcggaact gatagagttg ggcgaggccg aagcggttgg
     5581 cgccttcgat catcatgatt gaggcgttcg ggatgtccac gccgacctcg acgacggagg
     5641 tcgcgacaag aatgtccgtt ttcccgtcct tgaaattttg catggccgct tctttctctt
     5701 tcggtttcat ccggccgtgc aggaacgcca cgcgcaggcc gggaaatatt tttttcgaaa
     5761 gtttttcata ttcttctttc acggatttca cgtcttgaag cgcccgcctg tccgccgctt
     5821 cgaacattcc aaaaccgcca gcggttttgg aatggacaga tatccgcatt cgccgcgaag
     5881 ccgccgccgc cgcgttctcc gcatcggtcg gttcgacgcg cgggcagatc acgaacgcct
     5941 ggcggccttc ttttatctgc cgccggatga aatcatacgc gcctgcgcgc tcacggaggc
     6001 cgacgatttt cgtttcgatc ggatgccgtc cggcaggaag ctcggtgatg agcgagcaat
     6061 caagatcacc gaagaccgtg agcatgagcg ttcgcgggat gggcgtcgcg gacatcgaaa
     6121 gaagatgcgg cacgatgccg ttttttgttt ttgacgccgc ctggagcgcg gcgcgctggc
     6181 gcacgccgaa gcggtgctgt tcgtcgacga cggcaaggcc gagctttttg aaagaaaccg
     6241 atttttcgat gagcgcgtgc gtcccgaaaa cgatcgcgac gccgccgtcc gctatttttt
     6301 tgccgagatc ttttttcgat attttcgcct cgacgtcgtt ttcgtaaaat attaccgcgc
     6361 cgcttccggt catgagcccg atcacgggct gtgtgcgcgc ggcaatggtt gcgcatattt
     6421 tcttcatcgt ttcgaaatgc tgccgcgcga ggatctccgt cggcgccatg aacgccgttt
     6481 ggatcccgtt ttcggcggcg atcacggccg cgagcgccgc aacgacggtc tttcccgaac
     6541 cgacgtcgcc ctgcagcagc cggttcatgg gacgtgtttt ttccatatcc tgcataatct
     6601 cccagagcga ccgtttttgc gattgcgtga gcgcgaaagg aagcgcggca agcattgctt
     6661 ttattttttc gatgtcggcc gcaacgcgcg gcgccgtgcg ctcggcgcgt ttcagttttt
     6721 gattgacatt atgcaactgc aggagaaaaa gttcttcgaa cgcgaacctt ttttttgcgg
     6781 cgagcgcccg gtcgatcgta tcgggaaaat gaatagcgcg gattgcgcgg gacaattccg
     6841 gaagccgttc gcgcgcaaga atgtctttcg ggatccattc gacaagcggg ggactggctt
     6901 cgaatatttt ttggataatg aaccgcaggc cgcgcgacgt gaggcctttt gtttcgggat
     6961 agacggggac aagccgcgcg gtatgcttcg gttccgcgcc gtcatgcgcg atctcgtacg
     7021 ccggattgct gaaatacggt tcgccttcgc gcgaacgcga tatttttccg gaaaggttcg
     7081 ctgtttttcc gacgcccatg gcattcgcga tgtacggctg attgaaccag acggcgcgga
     7141 tggagccggt gtcatccgtg atcagcgcct ccgtgatggc catgccgcgg cgccacgccc
     7201 gttttgtgtt tatatcgtcc acgacgccgc agatggtcgc ttcctgtccc ggttcgaggt
     7261 cgtttatttt atagatcttc gaaaaatctt cataacggac aggaaagtgc cagaggagat
     7321 cgcggacggt cgtgatgccg agtttttcga gccgacccaa aaaagcggac ttcgatccca
     7381 gcgcttcgga gagcggcgcg tcgagcgcga tgcgagccat aagtgcgcat agtatagcat
     7441 tttcagagag ctaaaaacag cgcggtttaa attcatggaa cccgtctcaa aagcaaagtt
     7501 ctctataatt ccctccgtct ttcctatcag gatggactat ttttgcgatg ggctttaatc
     7561 tgatagggaa acgccctctt agttcaatgg ataaaatatc tggcttcgaa ccagaagatg
     7621 taggttcgat tcctacagag ggcacaatgg aaataataat acgacgctgg tctattttca
     7681 cgcgccgaaa ataaaaatgg gagcctcaat gaggtgtttc cctatcgagt gttgcgggtt
     7741 gaatggcaag actccaaatg aagcagtcgc cgaatttaaa aacttatccg ctataggtgt
     7801 gtatttaaaa cattcaggcg tcccaccaca ataaaaacgg ccggaagcga caatccttc
//
LOCUS       DAIDDT010000034         7758 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_34_length_7758_cov_6.796771, whole genome shotgun sequence.
ACCESSION   DAIDDT010000034 DAIDDT010000000
VERSION     DAIDDT010000034.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34_length_7758_cov_6.796771"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2013 a         2105 c         1887 g         1753 t
ORIGIN      
        1 tttttaaaga ataaaaaaac gaggttaaac attatcatgc cggagaaaaa aaagacggtt
       61 aaaaaacaac tgcctccccg cgctcccgca aaaaaacggc cggttaaaaa atccgcgccg
      121 gtaaagagaa ccgtcagaaa gaaaaaaatc gcactacgca ttccgcgccc gcccaaaaag
      181 aaaaaaactg cccgtcttga aaaatcggaa tataatccca ttatttcccc cctcggggaa
      241 cacgcatggg aatcgggcca aactttcaat cccgccgccg tgcgcatgga cggaaaagtg
      301 catctcgtct atcgatccat aggagacgac ggtatttcgc gcttcggtta tgccgcaagc
      361 tatgacggcg aacatgtgca tgagcggcat ccccatcccg tcttttcgct tttgttgaat
      421 accaaagaac cgcagccgca tcggatctac acatcgggcg gcagctgggg cggcgccgaa
      481 gatccgcggc ttaccctgct tgacggcacg ctctatatgt tctttaccgc tatcgacgaa
      541 ggaggatttc cgcgcgtcgc catgacttcc atcgcaaaag atgattttct ttcgaaaaaa
      601 tgggattggg ccgcgacgaa aataatctct cctcccggcg aggcacataa gaactgggtg
      661 ctattccctg aaaaaataaa cgggaaatac gccgtcctgc acagtatctc gccagaaatc
      721 tccgtcgagt acctcgataa tctcgatttt caagataccc ctcatttgaa aagttttttt
      781 aagccggccg gcataagcgg cggatgggaa aaaagcgtgc gcggcatcgg cccttcgccc
      841 atcaggacaa aatatggatg gctggtgctg tatcatgcct caagcgatga ccacggctat
      901 gccataggcg cgatgctgct cgatatcgat caaccggaaa agatcatcgc gcgatcgaaa
      961 gaaccggtca ttgaaccgaa ggtatggtat gaaaattacg gatggaaagg aggtattgtc
     1021 tacagctgcg gcgcggccgt catcggcagc aacctctttg tttattacgg cggcgccgat
     1081 tccgtgggat gcgtcgcaaa agtaaatctc gaagattttt tgaatgacct tatggatgcg
     1141 cgcaaaccga aactcgccac agcgatgcga aaaaaataat ttccgtcaat gatagtcaag
     1201 aatgtttaaa ggtcggctcc cgtccatcat cctcgacgct ggccggaaat ctatgctttg
     1261 ataaatagat acaatcatta ttgtgtcatc ctcgcggagg ctcgccctat ggagtggcgg
     1321 ctttgcctac tccacggggc aaggatccat gctttcggtc ttttctcttt atttttttaa
     1381 aacacagatc ctcctcttcg ggagaggagg gcgacagagg ggaaattata aattcttgac
     1441 cgccaaaatt tttcggagaa aaactcgagc cggcccgttt tccatttgac gggcacacga
     1501 caaaccgcga gggcgacaaa ctaagacaaa gaagaagaaa atttacaaat aaccttatga
     1561 tcaccgttac ccgatcgagc cataatccga tcctgaaacc ggaccgcacg aaagcatggg
     1621 aaagccaggc ggtctttaac ggctgtcctg tccggcacgg cggaaaaatt tttcttcttt
     1681 atcgcgcatt gtcattcccc cactaccaca cggaagcagg ggttcaattg agcgtctcgg
     1741 atatcggacg cgcggaaagc gaggacggtt tccatttcac gaaacggacg ccgttcatcg
     1801 tccccaaaga acattgggaa cggtttggct gcgaagatcc gcgcgtgacg ttcttcgagg
     1861 gacgctatta tattttttat accgcccttt ccgtttaccc gtttgcgccg gagggcatcc
     1921 gcgtcggcgt cgccataagc gacgacctcg cgaccgtgaa agagaagcat ctcgtgacgc
     1981 cgttcaacgc gaaagcgatg gcgctgttcc ccgggcgggt gaacggaaaa atatgcgcaa
     2041 tccttacggc tcatacggac atgccgccga cctcgatcgg catcgcgtta ttcgaccgtc
     2101 cggaagacct ctggtctcgg gaattctggc agcaatggga aaaaaccatt ccggaccacg
     2161 ccgtccctct tttaaggaat ccactggacc agatcgaagt cggcgcaccg cccttaaaaa
     2221 caaaagacgg ctggcttatc atctattctt atatcagagg ataccgcacg ccgaaaccgc
     2281 ttttcggcat cgaagcggcg ctgcttgatc tcgatgatcc cacaaagatc gtcgcgcgca
     2341 ccgacctgcc gctcatggtc cccgaagaag aatacgaact atacggcatc gtaccgaaga
     2401 ttatctttcc gtcggggatc cttgaagaaa aaaagaatgt tctgatttat tacagcgccg
     2461 ccgacacgac ctgctgcgtc gcatccgtga atcttgaaga actcatccgc ttcacgaaag
     2521 atacttttga agcgagaaaa aacgagttcc aggtgcggtt cgagcgggcg cccgagaatc
     2581 ccattcttgc gccggacccc ggccggccat gggagacaaa agcggtgttc aatcccgcgg
     2641 cgatcctgat tgacggacgc gttcatcttc tttaccgcgc catgggcaac gacaatacat
     2701 cggtgctggg ccacgcctcg agccgcgacg gcatacattt cgacgaccgc gatccaagcc
     2761 ccgcctatgt tccccgcgcg tcgttcgaga tgaagctggt tccgaacggc aattccggat
     2821 gcgaagatcc gcgcatcacc cgcatcgacg atacgattta tatgtgctac accgcgttcg
     2881 acggacggca ttcgccgcaa gtggcgctca cgtcgatcct cgtcgacaaa tttctgaaga
     2941 agcaatggga atgggcgccg cccgtcatta tttcgccgtc cgcgttcgac gacaaggacg
     3001 cctgcatttt ccccgaaaag gttggcgaaa attatatcat cttccaccgc atcggcgaca
     3061 acatcgatat cgcattcacg ccgacgcttg atttcaccgg agaaacaacg cttgaagaac
     3121 agcaatggct tgaacggagg gacggtttct gggatggaag aaaagtaggc gtcgccgcac
     3181 cgcccataaa aacggacaaa ggatggatcc ttatttatca tggcgtttcg gaatcatcag
     3241 ggatctatcg cgtcggcgcc gcgctgctcg acctgaaaga tccgtcgaaa ataatcggac
     3301 ggacggatta tccgcttttc gaaccgcaga tgccgtatga aaaaaacggc caggtggcaa
     3361 acgtggtgtt tccgtgcggc aatgtcctta tgggagaccg gatattcatc tattacggcg
     3421 gggcggatac cgtcgtcggc gtcgcgagta tcgaagtggc gtcgcttttg cgcgcgctga
     3481 cataaagccg cctcctctgc cgtcctcgcc gtgcgtggta aaaccccgcg agaacaactg
     3541 aggaaattct atccgccagc gacaaggcgc gctattttca aaatcgcaat aagaacaatc
     3601 tggatggcga tattcacaag gatcaaaccg agcgccataa aacccaaacc ggcgaaaaac
     3661 gctgacgtta cgaaaaagag gacgataagg acaggccata tgtttttcag atcctgcggc
     3721 gacggcccga tcatggcgcc ggcgttaaaa aacagaaaaa tcatcaccca gctttgccac
     3781 tgaaacaggt ttatctggag caaaaatcgc aacggcagga taaaaagatc ccgccaatgc
     3841 tgccagccgg aaaattgcgg cagcacgaaa taatttccca aaagatagcg gttcacgaaa
     3901 aaaagaaaca gcagtcctcc ggctatcggc gcgaacgata taagaacctc gcccaaaacc
     3961 ggcagtttcg attttcgatg caccacgcgg ggctgcgatg aaaaaaccgt gaactcttct
     4021 atcttcgctc cggtcacgac gcaaagcacc gcgtgagacg tttcgtgcac aaaagcgcct
     4081 atgtaataca aaaaacggat gacgccgaaa ttcaaatacc gccagttcaa cgcattgctg
     4141 gcaaaacccg cggccgtgat gagaacaata ataattccga gatttatcat ggcgcgtaca
     4201 gttttcaaaa cgccgcttat ttttgtttct tacttcttat tattccccgt ctttgcggct
     4261 cggcatattt ctcttagggc ttgcgcgctt ggccgttgcg gtttgggaaa ttttcttatg
     4321 atttttttca aatgcgcacg tcctatctct tctctaaaaa attttaattg cattccgcat
     4381 cataaacccg cttccgaagt tccgaggtcg agtatccgtg atcgcgggaa ttataataaa
     4441 cttccatagg caaatcataa cccgtgaact ttttaccttt ccaatccgcg ccgataatac
     4501 ggatatcggg tttcagtttt ttcaataatt cataaagacc gctttccccg tcgtaaggta
     4561 tgatttcatc aatgtaacgg attccctgca atatgataag ccgttcggcg agcgacagga
     4621 ccggcttgtt tttctttttt ccgcggtaat ccgcaggagt gtttgacgga tctatttcaa
     4681 gcccgaccat gagataatcg cagtgctccc tggcttcttt cagcatcaaa acatgaccgg
     4741 cgtggcaaag atcgaaagct ccgcacgtga atcccttttt cataggttcc ataatagcac
     4801 aaatgttcga cgccgcacgc gccagtttct caaacctgcg caaaactgcg attaataaaa
     4861 agcggaaagg gagggattcg aaccctcggt accttgcggt acacagtctt tccaggactg
     4921 ccgtttaaac cactcacgca cctttccaaa tgaaaactgt tgtagaactc gtctcaaaaa
     4981 tagcttttat aaaggcaaag tccgcggatc tcgcggttct ttccggctgc gaagcgcgat
     5041 ttcctcctcg cactatgatt atagcgctcg tcggaagatc gcgcttctcg ctcggaaagc
     5101 cctcgcgagt tccgctggcc tatttttgag acgggttcta gtgttgaaat attgtaatgt
     5161 tctaatggca aattcctgca tagcaattcc ccgacactgc gacacgggaa cactacaaca
     5221 ttaaaacatt caatccccga ttattgtgcc ctgaaaatcc tttccgttca agaggtcgcg
     5281 aaaattcttg attttccgtc cgtcgatgat gattgctttg atactttctt cttcgccaag
     5341 cgcggcgccg acgggatcca cgggcgcgtg caatcccggt ttccatttcc gcggaacgag
     5401 cttccgataa ttccgccagc tcaattcggg caacggcttt gcgtcgggat gcttcgcatt
     5461 atccttttcg tacacatgcg ccggcttccc ggcgatgatg accccggaaa cgccgtaatc
     5521 ggcggcgatc tggatcgcca cgtaatcggt tgaccatccc ggccgccatc cggatgcgat
     5581 cgttatcgga tattcaagat gcggtatttt cccgcggcta tcgatcacaa ccggatccgc
     5641 gagttcgcgg aatacggtcc gcagaagatg cgcattgagg cgcgttgcat ggatgccgat
     5701 ccaatccttg tcgtcatcgg tgattttcac cactgccgac gcggcgtcct gaaaacggcg
     5761 cgccaatgcg ccgccgccga tcacgatcac aaaccgcatg ccttttttga tgaatggcgc
     5821 aagaaatttt ttgaaatctt tcaggaatgc ggtattgatc ccgtccggat ggacaatgga
     5881 tccgccgagc gcgatgataa ccgtttttcc aaatcgatat ttcatatatt ttcgtttcga
     5941 tgcttatgct gcgcgcggca aagtccggtc acacggatat tttctgcaat gcggcgatac
     6001 gctcttcgat aggcggatgc gtcatgaata atttatgcca ccacgaaacc tgccggccct
     6061 taaacggggt atcgatccac atatgcgccg tggaattttt cgcgctcgcc atcggcgttt
     6121 cgtcatgctc aatcttccgc aaagcggcaa taagcccttc gggatagcgc gtgagcagta
     6181 cgcccgaagc gtccgcgagc gactcgcgcc ggcgcgcaat cgcaagctgg atcagcatcg
     6241 tgccgatggg cgcaagaatg gaaaggacga ttgcaaaaat aaaaaaaatc tctcctgact
     6301 caccgctgtt gtttccgcgc cgtccgccca taccgccgaa aaaaagcgat tgcaaaaaaa
     6361 tgtcggcaag caaagaaata attccggcaa gcaccgcggc gacggtcgaa acgagaatat
     6421 cgcggttccc gacatgcgac agctcatggg cgagaacgcc ctgcagctcc gttttatcaa
     6481 gccgctggag cgcgccctgc gttacggcaa cggcggcgtg cttcgggtcg cggcccgtcg
     6541 caaacgcgtt aatctgcatc tcgggcgtga tatacagctt cggcatggga agccccgagg
     6601 caatgcaaag attttcgacg gtatcccaca gcatcggatt atctttcttt tcgatttcag
     6661 tggcgcgcgc catggaaagc gcgatgcccg cggaggaata ataactgatc aaggaataaa
     6721 cggcgctgaa aatgaccgca aaaataagga aactgtaatc gccgtagata tacgaaaaaa
     6781 accagccgat gcccgtgacc acggcaaaaa acacgatgaa caaaagccac gtgcggcgga
     6841 cattggaaga ttgataggtg tataaattag ccataaaaaa tatgaatgct ccttgtcatc
     6901 cctgtgcttc tgccattatc tgcccttttt ctgtcatcgt ccgtccttga ttgtggtcga
     6961 tggcaggatc cggcggacga cccatacctc atttcgagct tccagctatt agctattaac
     7021 tcgaagagag catggatccc cgcctccgcg aggatgacag aaggaaaatc gagcgcaaaa
     7081 aatctaaaat ttaacctgca cggcctgctt ctccgcgtcc gtgagatcgt tgccgaaaaa
     7141 atccatctgc ttgaaaccga acgcattcgc gatgatgttc gacggaaaaa cctggatttt
     7201 cgtgttcaag tcgcgcacca taccgttata aaaccggcgc gccgcctgaa tcttgtcctc
     7261 ggtgtcggtc agctcgccct gtaaactcaa aaaattctcg ttcgccttca attgcgggta
     7321 gttttccgca accgcaaaaa gcgacttcaa agcgcccgaa agctggtttt ccgcgcccgc
     7381 cttcgcgggg cccgtcgcct gcattgcctg cgaccgcgcc gccgtcaccg tctcaaaaac
     7441 gcccgcctcg tgcgccatat atcccttcac ggattcaacg agattcggga tgaggtcata
     7501 ccggcgcttc aattgcacgt cgatgtccga aaacgcctct ttcgcctgat tccgcacgcg
     7561 tacaagcccg ttatattcga ccaccagcca cagcacgatg accgccagca cgatatataa
     7621 ataaatcatg gattgatgat aacagaaaag aagatgacat tgcaaacctc ctaccttgtg
     7681 gcctattatg ctcttccgaa agctttgtaa aacaaggtag gtaagcaagg tagatagata
     7741 ataaacatag gtaagata
//
LOCUS       DAIDDT010000035         7507 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_35_length_7507_cov_8.004711, whole genome shotgun sequence.
ACCESSION   DAIDDT010000035 DAIDDT010000000
VERSION     DAIDDT010000035.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7507)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35_length_7507_cov_8.004711"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1842 a         1883 c         2216 g         1566 t
ORIGIN      
        1 attttattat caacgttatg gcaaaattct cttcgacgaa aagattttcg aaatcaaaac
       61 cgctgattgc gccttccaac ctgaacgagg tgcagcttgt ttcgtacaaa tggtttctgg
      121 aaaaaggatt aaaggagctt ttcgaggaga tctccccgat cagggatcat accggccacg
      181 agttcgaact ttatttttcc gactatcgct tcgacgagcc gaagttcgac gaggtaacgg
      241 cgcgttataa agaggcgacc tatgaggcgc cgctccgctg tattttaaaa cttatcaacc
      301 gccagacagg gcgcgaggaa tcgcaggaag tgtacctcgg agattttccc gtgatgaccg
      361 atcggggcac gttcatcatc aacggcgtcg agcgcgtcgt cgtatcccag cttatccgtt
      421 cggcgggtgt ttatttcact tccgtcccgt atcgcgggcg gcagcttttc ggcgcgaagg
      481 tgatcccttc ccgcggcgcg tggcttgaat tcgaaacgga cgcggacggc cagatcggcg
      541 tgaagatcga ccggcgcagg aaagtcgccg tgacggacat gctgcgcatt ttcgggatgg
      601 ataatgacgc gattttgacg gcatttgaag acattgatac cggtccgata aaatttatag
      661 aggcgacgct caaaaaagac gcggcgaagg acgccgcggc atcgtatgtc gaaatttacc
      721 gccgcttgcg cccgggcgat cccgccagtc ccgagaccgc gaaaaatctc gtgagcgcaa
      781 tgttcgagcg cgccgaccgt tatgatcttt cggaagtcgg ccgttacaaa atgaaccagc
      841 ggctcggctt ttcgcgggag aaaaaggaaa agcttttgga taaagaagat cttatcgcca
      901 tcgtccgcga gattatttcc tcgaacaata atccggatgc cgagtccgat gatatcgatc
      961 acttgggaaa ccgccgcctg aaagcggttg gggaactttt gcagggccgt ttgcggatcg
     1021 gccttgcgcg tatccgccgc ggcgtgcagg accggatgtc cacggaagac aaggcgaccg
     1081 tgatgccggc gcaacttttg agtttccggc ccattgtcgc cgtgataaag gaatttttcg
     1141 cgtcgtcgca gctttcacag tttatggatc aggtgaatcc tcttgccgag ctcgagcaca
     1201 agcggagaat ttcggcactg ggtcccggcg gccttacccg cgaacgggcg agtttcgaag
     1261 tgcgcgacgt acatcgttcg cattacggac gcatttgtcc gatccagacg ccggagggat
     1321 cgaacatcgg tctcatcagc tatcttgcga gtttcacccg tgtgaacgat ttcggattcc
     1381 tcgaggcgcc gtacgcaaaa gtgaaggacg gcaaagtgac aaaagaaatc gtatggcttg
     1441 acgcgaccga agaggaaaaa tacaagatta cgcatgcagg catcgcgcgt gatgagagcg
     1501 ggcacatttt agggacattg attgaggcgc gcgtgaacgg cgaaccggca acctgcactc
     1561 cgaaagagat tgatttcgtt gatgttgccc cgaacgaatt tatcagcgtc gcgacgtcct
     1621 taatcccttt tctccagcac gacgatgcct cgcgcgcgct tatgggttcg aacatgcagc
     1681 ggcaggcggt cgcgtctgta aaaccggatg ctccttatgt ttcgacgggc gaggaagacc
     1741 gggtggcgct tgattcgggc catgtggtcg tggcggaaga agatggcgtt attttggaag
     1801 cggatgccga tcatgtcaaa gtgaaatatg cgaaaacggt aaaaaaccac gttctcgaaa
     1861 aattcaagcg gtcgaaccag ttcacgtgta tttcccagcg cccgcttgtc ttaagcggag
     1921 aaaaagttac aaagggccag attctcgtgg acggtccctc catcgataac ggggtgcttg
     1981 cgctcggtca gaatttgctg gtcgctttcg tttcgtggga aggcgccaat ttcgaggacg
     2041 ccattatcct ttccgaacgc gtggtgcgcg aagatcgttt tacgtccatt catatcgata
     2101 actacgattg cgacgtccgc gacacgaagc tcggcgcgga agtcacgacc tacgacattc
     2161 cgaacgtttc ggaagataag ctgaaaaatc tcgatgagga aggcattatc cgcatcggcg
     2221 cggaagtgaa ggccggcgat atcctggtcg gcaagatttc gccgaagggc gagtcggacc
     2281 ttacgtcgga ggagcggctt ttgcgcgcaa ttttcggcga gaaggcgcga gatatgaagg
     2341 acgtttcgct caccctgccg cacggaaaaa tgggacgcgt ggtgaacgtt aaaattttcg
     2401 atcgcgagcg cggcgaccgg ctggatcccg gcgtcatccg gaaaatccag gttgagattg
     2461 ccgaacttcg caaagtccag gcgggcgaca agctcgcggg gcggcacggt aacaaaggcg
     2521 tcatctcgca ggtgcgcgcc gtcgaagaca tgccttacct cgaggacgga acaccggttg
     2581 atattatttt gaatccgctg ggcgtcgtat cgcgtatgaa tctgggacag attctggaaa
     2641 cgcatctcgg ctgggcggca tcgaagctcg gttatcgcgc aatcacgccg gggcttgatt
     2701 cggcgaccga agaacagatc cgcgaagaac tccgcaaagc gggcttgccg gaggacggca
     2761 aggcgaaact tttcgacggg cgcaccggcg aggcgttcca taaccgcgtg acggtcgggc
     2821 agatttattt catgaaactg aaccatcttg tcgaggataa ggcgcatatg cgttccatcg
     2881 ggccttattc tttgatcacg cagcagccgt tgggcggcaa ggcgcagttc ggcggacagc
     2941 gcttcggcga aatggaagtg tgggcgctcg aaggatacgg cgcgcggcat acgctgcagg
     3001 agatgctcac gataaaatcg gacgacgtga tgggccgcgc ggcggcgtac gaatccatca
     3061 tccgcggcga gccgatcagg gaaccgaacg ttccggcgtc tttcaatgtg ctggtgaacg
     3121 aactgaaggc gcttgcattg aacatccagc cgctttacgc gaacgcggaa acggatcgga
     3181 aagacgattt ctccgccatc aagattggcg tggcatcccc gcaggatatt ttgcggtggt
     3241 ctcacggcga ggtcatcaag cccgagacga tcaattaccg gacccagcgt cccgaaaagg
     3301 acggactttt ttcggagcgc attttcgggc cgacgaagga ttacgaatgc tattgcggca
     3361 aataccgccg catccgctac aagggcatcg tctgcgacaa gtgcggcgtg gaagtgacgc
     3421 gcgccgccgt gcggcgcgaa cgtatcggac acatcgcgct cgcatcgccg gtcagccata
     3481 tctggttcct gaagagcgtg ccatcgcggc tgagcttgat gctcgatgtt tccacgcaga
     3541 aactcgaacg ggtcatttat tactcggcgt atatcatcac ggacatcaag gaagagaacc
     3601 ggaaacaggc gcttgaggaa ttgaatcgcg agctcaaagg aaaactgaag acggtcggca
     3661 ataaagacaa gacggtgaaa ggggagatcg aggacgccgc cgatacgaca cgtgattatc
     3721 ttgaagatat gcgtatcggg cagattttga gcgagaacga atatttcacg ctgtcgcgga
     3781 agttcgggaa tatcttcaaa gcgggcagcg gtgccgaagc ggtacggcag attttggaga
     3841 atatggattt gcggaaagag gtacgcgccg tcgatgccca gctcgagaaa gcgaaagatc
     3901 ccatggcgca aaacaaactg ctgcggcgtt taaagatgct gaaagcgatg attgagaacg
     3961 gcacgcgtcc ggaatggatg gtactcacga cgcttccggt cctgccgccg gatctccgtc
     4021 cgatggtgcc gctggacggc ggccgttatg cgacttccga cctgaacgat ctctatcgcc
     4081 gcgtcatcaa ccgcaataat cgtctgaaga agctgcttga gatcaaggcg ccggatatca
     4141 tcgtgcgcaa cgaaaaaagg atgctgcagg aagcggtgga tgcattgatc gacaattcgg
     4201 cgcggttcgg cgtgcagcag ctttcggcgc agcgccggcc gttgcgttcg cttgccgaca
     4261 tgttgaaagg aaaacaggga cggttccgcc agaaccttct tggtaagcgc gtggattatt
     4321 ccggccgttc ggttatcgtc gtcggcccca agctcgcgct cgacgaatgc ggtctgccga
     4381 aacggatggc gcttgaactg ttccggccgt tcgtcatcag cgagatcatc aagcagggat
     4441 tggcgcacaa tatgcgcagc gcaggccatt tcattgaaga gcataccccg gaagtgcttg
     4501 cgattcttga agagatcatc aaaacgaaac acgtgctttt gaaccgcgcg ccgacactgc
     4561 accgtttgag cgtgcaggcg ttccgtccgt tcctcgtcga gggtcttgcg atcaagatcc
     4621 cgccgctcgt ttgcaccgcg ttcaattccg actttgacgg cgaccagatg gcagtccatc
     4681 ttccgctttc ggatgccgcg cagaaagaat cggcggagat catgacggcg ggcagggggc
     4741 ttttgaaacc gtcgaccggc gagcttgtcg ccgcgccgac gaaagatatc gttctcggca
     4801 tctattatat tacgcgcggc gatctggaat ccgaaaaacc gcggccgatg tcgagcgcgg
     4861 aagaagcgtc gcttgcctat aacgccggcg tgataggttt caagacgccg atctttataa
     4921 acgggatcgt gacgacctac gggcgtctca tctttaacca gtcattgaac ggcaaggttg
     4981 attttgtgaa cgaaacactg aatgccccca agctgaagaa aatcatagag cggattatcg
     5041 acgcgcacgg catcgacatc gcgcgggaag tgctcgaccg cgtcaaactc ctcggcttcg
     5101 agatggcaac ggtatcgggt attacgtggt caatggcgga cctggtgatt ccgaaggaga
     5161 aaaaggacat catcaagaaa gcggatgcgg aagtcgatct ggtacaccgg cagtttgcgg
     5221 aagggctttt gacgcccggc gagcgccgct cgcgcgtaat cagcgtctgg gacaaggcga
     5281 aaatcgatat cgcgaagctc gtttccgtcg tcctgtcgcc ggagaacaat atttatcaga
     5341 ttatcgactc gaagtcgcgc ggctcgtggt cgcagccgat ccagatgatg ggtatgaaag
     5401 ggctggtgca gaatccgaaa ggcgagacca tcgaactgcc ggtgaaatca tcgctcaaag
     5461 aaggactttc ggttctggaa tatttcattt caacgcacgg tgcgaggaaa ggaacgaccg
     5521 acacggcgct gaagaccgcg caggcaggat atcttacgcg ccgtcttgtc gatgtcgcgc
     5581 aggatctcgc tgtccgcgag ggggattgcc ggtcaaagga aggcatcgaa cttttccgcg
     5641 ccgacggcaa ggaattcaac cagagcttcg cgtcgaaagt tttttcgcgc acggcgctcg
     5701 atgatatcaa gataggaaat aaagttatcg tgaaagcgaa tgagatcgtc gacaaggccg
     5761 cggcggaaac aatcgaccag tcgaagctcg agtcggtcat cgtgcggtcg cccatcacgt
     5821 gcaaaacgct ttacggcatt tgttcgaaat gctacggcta tgacctggga agcaacgagc
     5881 cggtggtgat aggaacggcg gcgggcgtta tcgcggcgca atccatcggc gaaccgggga
     5941 cgcagctcac catgagaacg ttccatacgg gaggtattgc cggcgccgac atcacgcacg
     6001 gcttgccgcg cgtcgaggaa attttcgaaa tgcgcctgcc gaaaggccgc gcgatactcg
     6061 cttcgttcga cgggaagatc gacaggatag aggaaaaagg cacgctcaag atggtccgca
     6121 tcatcgccga cgacaaaaaa gcgaaagcgc aggaaatgaa tatcccgcgc ggcacgatcc
     6181 tgttcgtcaa ggcgggggac aaagtgcggc agggagacca gctttcggaa ggcaaccttg
     6241 atatccacga actttacgag cataagggcg tgatggaagt ggcgcgttat atcgtgaacg
     6301 aagtgcagaa gatctatatg tcggaaggcg cgccgatcaa caataagcac atcgaaatga
     6361 tcatccggca gatgttttcg cgcgtgaaaa taagcgcgag cggcgacgtc tccgacttca
     6421 tcatgggaga ggtcattgaa aaatcgaagt tcctcgaaac aaataaggcg gtcaagaaac
     6481 agggcggaac gcccgcgaaa tcggagcaga tccttctcgg cgttacgcgc gtcgccttgt
     6541 ccgcggacag tttcctttcg gcggcgtcgt tccaggatac cgcgcgcgtg cttgtgaagg
     6601 cggccgtcga atcgaaagtg gacaagttgc gcggcctcaa ggagaatgtc atcatcggcc
     6661 gtttgatacc gatgggaacg cgggaagcga tcgccgtcga tacgctcaac gagatggagg
     6721 aggaggaaac attgccgtct ccggtggcgg tgaaggagta aaggagtaga agagaagata
     6781 gagcgttttg tttttctttt ctgtcatcct cgcgacggcg aggacccatg tttttctatc
     6841 ttggtatttg gaactggaat ttaattttct attgagtatt tgatcggagc aaagatggga
     6901 aacgtgggtc gttcgccttc gcggacgatg acaagtggac gcttgcaatc gggcatttat
     6961 ctggtacaat acaaaaacta tggaagacaa cgaaaaagac aagaagaact acgaaatttc
     7021 ctttctcgta aaaagcgagg acgatattcc cggcgtggtt tcgtttttga gccgacataa
     7081 cgctgaggcg gttaccgagc cgcgcgccaa aaaacttgcg cttgcgtatg aaataaaagg
     7141 aaacaaggaa gcggtgttcg cgtattgcca ttgcagggcg tccggcgccg acgtgaagaa
     7201 tctcgagcgc gattttatca tgcgccacga tgtcatccgc tcgcttatca tttcgctttc
     7261 ttcaaaaaaa caggaagtcg tccggaggga tattccggaa agaaagccga tatcgccgca
     7321 gtattccgcc gcgccgcgtc cggaaatgaa aacgtcatcg cccgtgatcc tgagcaacga
     7381 agcactcacg aagaaaatag aggaaatact taaatagcaa aaagtgaata gtgaaaaatt
     7441 tcagaaatgc ttaattatta aattttctta aaccaaaact tttcgttttt cacttttagt
     7501 tttaaac
//
LOCUS       DAIDDT010000036         7484 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_36_length_7484_cov_6.295801, whole genome shotgun sequence.
ACCESSION   DAIDDT010000036 DAIDDT010000000
VERSION     DAIDDT010000036.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36_length_7484_cov_6.295801"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2123 a         1943 c         1784 g         1634 t
ORIGIN      
        1 atttgacaaa tatatatcct gtgctactat agaaacagtt tcttgaaaac ttaattgcgt
       61 ttgccgtaac agagaggaac tctttatctg gtaaagggtc tttgtctgtt atggcaaacc
      121 gggaattgtc ctggagcccg ctgactgaaa tgaggaggtt ttatgaagaa attaacggaa
      181 atagcaataa tagtaatagc tgtaatccta ctcgcaatgt ttttgaagga gatgggagtg
      241 tttggtccag tagtccatca tataattgtg gagtaagttt attttatacg ggctcttcgc
      301 aatcacgatt gcgaagagcc cgttttttta tgccgctttt tagcagaggg agaggagaac
      361 aggaaagtct ttttaggaga aagccccgtg gcatctttcg cgtttttttg ccatcaaaaa
      421 atttccgcaa atccgcttcc atggtaaaat tatggcatgg cattgcggag acgcgtggat
      481 aaacgtgcgc ttgtgcggcc cgtgtattcc ctcttgccat aaaatattat ataattggga
      541 taatgcctga cgtaaaaaaa ctgattgcgg gatttttgat attggcggcg ggagcaagct
      601 cgtccgttct cattttttcg gatctcaacg ccccgtccgc ttcccgggaa ctcgcggcaa
      661 ataccggttc ttctcccgcg cttggcaatg ccttcgcggc acagccgtcg gacaatacgc
      721 tgacaccgga aataaccaac agccccgata atctcacggg aaacctcact ggcgtgatat
      781 tgaacggcat tatggccgca aacccgcagg gtcccataac ggccgcgaac ggcgatcaga
      841 ttatgactcc accggatcaa aagacgatgc ttgcggagct ttccagcgat ccgtcattta
      901 aaaacttcaa agcacccgac tgggacgcgg aagccgcggc gcagaaattg aacatcgtaa
      961 gcaatgccac gtccgccacg atcgcgtatc tcacggattt caacaatatt tttcaaaaag
     1021 acattgcgca agccgacatc caaagcatcg taaatcagaa tcaaaatacc aatgccgacc
     1081 cgaattccct cgccgtcgta aacaacgcca taacggacgc gtttaacgaa gccgtacaga
     1141 taccgacacc ggtcaatctt gttgatttcc agaaaagctt tataaaattt ttgacgtacc
     1201 aaaaaaattc actgcaaacc gtccagaatt ataacgacga tccgataaag gcgtcgctta
     1261 ccctccaggc ggaaagtagc cggtaccagg cggcggtcag ccagtttgaa aacgcgggcc
     1321 aaaagattaa gaaccttgca ctggaaagcg accttaacaa gggaatttct tcgggagatc
     1381 cggatgccgg aaaacaagac agtacactcg ctttttttaa cgccgtcctt ggcatatcga
     1441 ccgcctacgc gatacccgta gaagttacct ctgactggat agaggcgctt aatctcatca
     1501 aaaccacact tacggcatcc gccacatggg aacagattgg ccttaccttg gaaaaatatg
     1561 ctgaacagct cgcgcttcag cttgttaaga acgcccttgt ttttgttatt caaaataaag
     1621 tgttggcatg ggtgaatggt agcggcgcac cgcgattcat aacgcagtgg gcaaacgcct
     1681 taattaatgt cgccaccgca aaagcgctta atacactgga tagcatgacg ccgcttgtct
     1741 gtccttcttt tggcccattg ataaatgcat cgcttaacgc gtcgctatca ctaaacacat
     1801 cattagaagg gttccagaca tgccccgcgc ccatgatggg taccgcccta caagatttta
     1861 atgataattt tgcaaacgga ggctggaatt cgtactactc catgctccag ccggaaggca
     1921 atttttattc ccaatatatg gacatatcgg atcagatgga cgcggcggca agcgctcagg
     1981 aaagtgccgc cacagcgcag gcaacggcaa accaggggtt cacgggcgat gcaacgtgcg
     2041 ccgatggaag caacccgaat ggatcgcatc agatctgcgt agatgcaatc ggcgctacga
     2101 ttcccacggt tgaccttagt aatagcgcca acctaacgct ttcttctcct tccgcgctca
     2161 gcttgggcgg cggcaacgca acacttccat cacttccgac gcaaggatgt tccgccggac
     2221 aagtaccgga aacaatacca aatggaggtt tatgcgccga tggaaccgaa ccgaccgtga
     2281 cgaccccggg gcaagcgacg ggccagatac tctccaaagc attgggggct aatatcagtc
     2341 tcattgtaaa cgcacaaaat ataacgggca tcatcacgtc agccgccgaa tcgctgatgt
     2401 cgaatatcat gcaaaccgcg gaaacgtctg ttatgaataa tataagcggc tacggtgaga
     2461 acaacggagg aatgaccggc atttacgaca atacattgtc gcagacgccc ggctggaatg
     2521 tgtcaatacc gctatcgtgc agtcccgcaa taacaaccac gcaaattcaa accacgcaaa
     2581 atagcaacgg caacagtaat gtgagcgcgc aattcagcgt cgccgcaacc ggggacaccg
     2641 accagtatgg caacgcgcca acctatatat ggagcgcgcc cggagcaaac ccttccgccg
     2701 gatacggcga cacgtttggc ggatcctata acaccacagg gattcacacc atcacggtta
     2761 ccgctccttt aatgacggac acgactcaaa cagttacgac aacctgccag gtcgtcgtaa
     2821 ccaccaacgc gccggtatca tgcagtccga cgatgcaaac cgtttatctt ccgataacaa
     2881 gagcgacatc gtccgtgagt gcgactgtaa ccgcatccaa cggcagtacc gaatacaacg
     2941 gcagtattat cgcgccgacc tacacgtgga ccattgcgcc ggacgccgta tcctcgttga
     3001 tctccggcaa tgaatcatca actttcaccg gaaccttcga cgcgccggga acctataccg
     3061 cgtccgtaac cgcgtccacg gacaacacat cggcaagctg ccagatcaaa gtggaacagc
     3121 aatcgaatac aacccaaccg taacaagcca aaacaacggg ggataaaaac atatggagca
     3181 tagtccggac aagaaaaata aaagaacctg gccaaagaac gggcttttga aaaagctctc
     3241 ttttgcgttc ccgatcttat tcgtcgtggt actggcggtg attatgccga ctcatgcggc
     3301 gcatgccggt attttgggga gcttaattaa ttttggctat aacgtgatag ggaaagttgt
     3361 gttctttatc ggctacgtca tttcttatat gatgggaatt tttatcggca tagaagcgtg
     3421 gcttatcggc gtgatactca atatgaacga caacgttttc caatccgcga tcgtccagac
     3481 gggattttct gcgtcgcttt ctctcgccaa cctcggattc gttttcggca tcatcattat
     3541 cgccattgcg accattctcc ggagccagac ctacggcgta aaacagatcc tctggaagct
     3601 cgtcgtgatg gcgatcctca tcaatttcgg cctcgtcatt atgggaacca tctttaattt
     3661 cgcggaccaa ttcacatcgt atttcctggc gtgcatcgat cctacaggcg gtgggtgtaa
     3721 cggcgcgaaa agcggcctct caagcgatag cgactttgcg accagtattg ccggcgcgtt
     3781 caatccgcaa cggagcttta cgtcagtaaa cagcgtaaat caaactattg gaactggagt
     3841 taataatggt ggcacggcga cgagcagcag cgtcttcgcc agttcaggga cggatatcag
     3901 taaaatgctt gttccgatat tttcggtatt ttttatcgcc gcatcgctta tcgtcatggt
     3961 tatcaccctc gcggcattta tcattatgct tcttatccgc tacgtatata tcgccatcct
     4021 cgccgtcctg ctgccgttcg catggatgct gtggatattc ccggaaacaa aaaataattt
     4081 cgataaatgg tggcacgcgt ttatcaagtg gacaattttt gctcccgttt ccctcttttt
     4141 catatggctt gcgcttacca cagcacacgg aataagcagt ggctacggaa ccggaaccca
     4201 aaacttcgct caatattata gcagcaatga taacgtctgg ggatcaatat ctggtttttt
     4261 tggtaatctc attactccta ttatccaaaa cttgctgaac gaaatcgtcc ttgtcggcct
     4321 tatggtcggc ggcatgattg cggcgaattc gctcggcatt acagccgcca acggggcgat
     4381 tgcttacatg cagaaaaccg gaaagggcgt cgggaattac gcaaaaaatt acgcaaaagg
     4441 aagagctcgc caggcggcaa cggcgcctct tcgtaccgag aaggcgcggg cggggcttac
     4501 ttcaatgcaa cagcgaaaag gcataaaagg actgccttcc cgctggatgg gaagactggg
     4561 cgaaagcatc gccgtaggcg gaggcgaaaa agcagccgcc gaacacgaaa aaggaataag
     4621 cgggctaaca aaagagcagg cattgaatca gcttacgaca tcgagcgctc cgcgcaggca
     4681 ggcgattttg aaacgcgccg aaaaagaagg atggcatgac gacgcgcgac tggatccgca
     4741 tcttgcggaa ggaaagatgg gtgaagcaaa acgatacggc aacgaaaaaa cattcggaga
     4801 gcttcgcgac aagagcggcc gttcattggt tgacgccata aaagcgaaaa agatcgctga
     4861 cgaaacaaag gatccggaca aaataaaaga agctgacgaa aaaatttccg gcgaagtgaa
     4921 aaagctcggc acaaaaaacc ccgacgcgct cgcttcgttc tttgtaagcg ataacgcccg
     4981 cgaaaaaata gccgacgaac tcggcaaaaa aggaattgcc gtaccggtaa cattacagcc
     5041 ggaatccatc cagggcgcgc aaaaagaaat cgtgaaaaca ctgccgacct tctcccagtt
     5101 caatgcatcg tcgcttctta ccgaaatctc caaaaagaat aatttgcctc aatttgaaaa
     5161 agcggcggcc agtcttactg ccgaggaaaa agaagcggct aaaaaagcat ttgaagaaaa
     5221 cacaacgctt atgaaatggg ttaaaaaggg ttcttaccgg acgatggttg acctcaataa
     5281 agcatatggt cttggcgtta aaaccgagga cgacgaaaaa gaaggaaagt cggcagggct
     5341 cgtggatcaa tttggaaggc cgttatcgta ataaatattt aaccaaggat tccttatgac
     5401 taccattacc acggctcaag cgagaaaaag atttacgctc cttcctcagg aactgcagaa
     5461 ttctattttt tctttcggga atgccgaaat tatagataaa atcggaatcg ataatcatct
     5521 ttccgaagaa aaaacggagg tggtggcaaa cgccgtatcg ctcgtccttt ttggatttat
     5581 ccatcaggaa gatcttgcgg cggaaataca ggaacaagcc ggtattccga aagaggcggc
     5641 tcttgctgtg gcgggtgaag cagatgaaaa aatattcaat gccataaagg acgtcatcaa
     5701 agaggcttac gctccggcta cggaagaaga aatgggagca acggcggaaa aaccgttgaa
     5761 aacagcgaat agcggcgggc cgatgatgat cgaagaaatt ccaatgaccg cgatgccggg
     5821 agcggcaacg gcggcgtctc aaaaacccgc accggcgccg aagtttccca taaaaaattt
     5881 aactgtggaa atgccgggag cgactccttc tgctgttccc gcgcctgccc ctattcccac
     5941 gatgccgaaa atcgctccgc aaatcttcgc cggaaacccg caaattcagg catcgttgcc
     6001 gctctcgaaa acccctgaga aaaaaccgga aactccgacg ccgaaaccgg tcatccttca
     6061 ggaagcggcg gcgttcgaaa caaacaaaaa atcggcggaa ttccatatcg aaacatccga
     6121 agacaaattc caaggattat cgaatattcc gcgcccgatg ccgcagaaag ccgccgtact
     6181 tgaatttggc tcatcctcaa aaacgccggt gtcgggctcg gttccttcaa agaattcccg
     6241 gccttcgcaa gatttcggcg ggcaggcaga agcgcgggca ggtatgcctc caaaaccggt
     6301 aaccggaaaa tacgaaggcg agttcaacga atttccttca ggaccggcgc ccgtcacgga
     6361 aaaaggcaga acgattacgg aaattacttc gtcttttcag gcctcttcaa aaaatccccg
     6421 gccttcgcaa gatttcggcg ggcaggcaga aaaaaataaa aaccaatctt ccggaggaaa
     6481 ttcagtaacg cccgccgcgc ccatgccgcc cgcgtcagaa atttccacgc catctaaaat
     6541 atccacagga agcgcggaga taaaaaattt cggcggcatt ccgcccatgc cgcagcatcc
     6601 gcgaatccag gaaaaggatc ttatgggaga attcgccgcg ccggcgcaga ttccaacgga
     6661 aaaaccagcg gataagaccg tgcccgcgcc ggcgccgttt atgataaacg cgataccggc
     6721 tcccgctccg caatcttctt cgcctattcc gccgccgcct ttgccggttt ttgggaaaaa
     6781 tatcctgcct tcacaaaatt cccgcttcgc ggaagcccct ggcgtagcac agggcggcgg
     6841 gcaagcagga aagtccgtat ccgacgtgac gaaattcact gcggaaaagc ccgctcgcgg
     6901 aattgtgccg ccgccgtcat tcgcaatgcc ggcacccgct ccccgaccgt catttttctc
     6961 taaaattttt aaaaaagaac tggcaaagcc ggccgtcgtt attcaaaaaa attattccga
     7021 agccgatacc gttccaaaaa tttcttcgcc gtcccaagct gatccgaaaa atacgggacc
     7081 gcaggccgtt tcgacaagtc cgacaaatcc ccaaaaaact ccttcgcctg catcgcttcc
     7141 cgatattccc gtcccgccaa aaaagaatta gaacccgtcc caaaaagaga ccggcggaac
     7201 tcgcgagggc tttccgagcg ggaagcggca taaaaaaaat gggctattcg caatcgtaat
     7261 tgcgaatagc ccattatgtt ttacacttcc cgccaatggc cgtttatttt tattaggttc
     7321 accttgaacc tagttgtttt aacagtacca gcaggaccga agaatgaatg gacgaccccg
     7381 ccgaaaaggt tccatacctc caaactgtaa ctcttcttat aaagaagagt catttctatt
     7441 gtacagcgat aaaacacttt ccggttgttg ttacattcgt tttt
//
LOCUS       DAIDDT010000037         7353 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_37_length_7353_cov_6.314596, whole genome shotgun sequence.
ACCESSION   DAIDDT010000037 DAIDDT010000000
VERSION     DAIDDT010000037.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37_length_7353_cov_6.314596"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1802 a         1784 c         2082 g         1685 t
ORIGIN      
        1 tatttataga atatttttcc ttttacaaaa atataggtga tggaatccct tcccggcgaa
       61 agaaaggttg tcttgttttt cgtcaattgt aaaaactgat ttttattgac gaaaacttcc
      121 cgcgttccga caagttgcaa tacctggaca tggcgcccgg gcatgatgga aatctttttg
      181 atgctattgt cggcataggg gaaaaaagca actatagccg tcaacgtgcc tatcaaaaaa
      241 accattatca agagatgtag tatggatctc aaaatcgcct ctcccttcga aattcccaat
      301 tctttttcga atctttgcac caatcctttc atcgctcctc cctttttaat attaaatttt
      361 aaaagaacaa aagcaagtat agtacacttt cgcattattg tcaagcgtgc cggaggaggg
      421 agtcgaaccc tcatgacctt gcgatcgatg gattttgagt ccattgcgtc tgccattccg
      481 ccaccccggc gttttgaacc cgtcccgtac attgataata attatttatc gcggtaaaat
      541 caaggcatcg cttcgattta taatgagcgg cgccggacgg caaatagtca caaatagttc
      601 aggattttta atactttttt aatggcacgg gttatcgcaa tctgcaatca aaaagggggc
      661 gtcgggaaaa ccaccacgtc cgtcaatctt ggcgcgtatc tcgccgtcgc ggggcggcgg
      721 gtcttgctta tcgatttcga tccgcaggcg aacgcgagtt cggcgcttgg gcatgaccgc
      781 gcggcggaca acgggagtat ttaccacggc atcaccggag aaaaaagcta cgaggaactt
      841 cggcgcgcga cggtcatcgc gaattatcat ttcattccgg gcgcgtcgca tctcgcgggc
      901 gctttgatcg agctggtgga cgaaccggaa cgggaatatt ttttgcgcaa attcatcaac
      961 cgcctgcggc acgagtacga ttatatcctt atcgatcttc cgccgagctt gagcctgctc
     1021 accgtgaacg gcctggtcgc ggcggacgag gttatcattc ccgtgcagac ggaatattat
     1081 agcctggaag ggctcggcca gcttttggaa actgtcgagc tcatcaaaaa caatctcgac
     1141 caccccatct gcgttgcggg agcgctggtt acgatgtatg accggcgcga acgcctgagc
     1201 cgcgatacgg taaaaagcct gcgcgatcat tttccgcatc acgtttttga aattgagatc
     1261 ccgcggagcg tcgcgctggc ggaagcgccg agttttgcga agccgatttt attgtatcgt
     1321 cccgacacga aaggcgccga tgcttatcgg agattagccg acgaggtcat cgcgcaggag
     1381 cggggttttg cgatacagga gggagtattc gcgagcaagg atttcggaaa tttaaacgta
     1441 ataaactgaa aagttttaat ggcgcggtgt tatggatgtc ataatgcaaa caattcaaaa
     1501 aaaatattac agcacttcga cattaaaaca ttaaaacgtc atcatgcttg gcaaaggact
     1561 tgaatcatta ataccgcgga agggaaacca gaatccgggc gggcagggct ccggaacgga
     1621 cgattacggg ataccgccgg tttttttgcc ggtcggggac agtatggatg atcccgcgcg
     1681 gtcgcttccg gtcgtttccg aagaactcga acttcccgcg ccgcccgaag aaaaagaaat
     1741 aacacgaccg gcggcgcaaa acgaactttc ttccgcagaa gaagactatg agcttcttga
     1801 gccggatacg cttcccgctt cggattcgcc ggcggttccg ttgcctgcgg catccgcgct
     1861 acgcaatccg gtttccggca cgccggcggt tttcgcgcat ggcgccgatt cagccatcgc
     1921 ttcggttttt tcggcggcga cggtagcggc agcagcaggc gcttcacgat catcgaagaa
     1981 aaaagacgat caagtgcagg aatcgatttt tcatatcgag gtcgatcgga ttgcgccgaa
     2041 ccccggccag ccgcggcggc atttcgacga ggccgcgctc cgcgagcttg ccaattcgat
     2101 ccgcgagttc ggattcatcc agccgctcgt cgtgacgaaa aaagagatcg aaacgccggc
     2161 gggcatcgat gtttcctacg agctcattgc cggcgagcgg cgtttgaatg cggcgaagct
     2221 gcttggcctc ccgctcgtgc ccgcgatcgt gcgaaaagtg aatctcgaac gtgaaaagat
     2281 cgagcttgcc attatcgaga atattcagcg cgaggcattg aatccgatcg agaccgcgcg
     2341 cgcgttccag cgtttacagg aggaattccg gatgacccag cgcgagattg ctgcgaaact
     2401 cggcaaaagc agggaaacgg tggcgaacag cgtgcgctta ctcgatctgc cggcgtatat
     2461 ccaggaggcg cttcagaaag gcgcgctttc ggaaagtcac gggcggctgt tactggcaat
     2521 cgacgaccct gcggcgcaga aaaaactttt cgatgacatc gcattgcgcg gacttacgac
     2581 gcgggacgtg aaggaacggg tgcggatcat cacgaagcgt ccgcgcgcgg gcggcgtcca
     2641 cgatataacc ggaggcgatc ttacgccgga gctcaaagcg atgcaggatc acctgagcgc
     2701 ggcgcttggc gcgccggtcg agatccatca gggcgccgcg aacggaaaaa tcatgatcac
     2761 attttattcg gaagaagaat tggataatat tttgcgacgg ctgggaacgg aggaatagaa
     2821 ttgagaaccc gcctcaaaaa tggtttccgt tattatttct gtttattttt taaatttttt
     2881 tcagaaaaac ccggaggatt tgcctcggga ttaatttctc tctataatct ttttacgcgg
     2941 cgccatcgtc tagcctggtc aggacgcatg gttctcatcc atgtaacacc ggttcgaatc
     3001 cggttggcgc taccaaatcg gatttcgtac cgtcatattt ccgggtaagc gtcgcaagcg
     3061 cgcgttcatt tcgcggttca atttttttta ccgttcatac tgctattatg gtagaataac
     3121 ggcatgttca aaaaaattct tattgccaac tggaaggagc atcccaagag cggacgagaa
     3181 gcggtgaaaa ttttcggcgc ggcgatgagg gcgaagacgc caaagaatat aaaaactgtt
     3241 gtttgtccgc cgttcgttta tctggaaaag tgcgcgagta cggcggtacg ccgcccgaat
     3301 gcgaaaggcc gcgcggcgct cggcgcgcag gatgttttct ggaaagacga agggccgtac
     3361 acgggcgaag tggggccggc gatgctccgg gcgctgggcg ttgcgtacgt cattatcggc
     3421 cattcggaac ggcggcgatg gctcggcgag accgatgcga tggtcaataa aaaaatcgga
     3481 gccgcgttgg gcgccggatt gcgcgtggtg ctgtgcgtcg gcgaaccggc gggcgtaaga
     3541 aaaaaaggaa ttgccgcggc gcaaaaattc gtgaaagacc agctcgcgaa agatttaagg
     3601 ggcatttcgt ccgataaaaa aatagcgcgt cgcgttgtcg ttgcttatga acccatctgg
     3661 gcgatcggca ccggcgacag tgccgatccc gccgatgtcc gcgcgatggc ggtatttatt
     3721 aaaaaacaac tgtccttcct ttttaaatcg ccggccgcca gccgccggtc gttcgttctt
     3781 tacggcggtt ccgtaaatgg aaaaaatatc gccgattatt tacaatacga agcagtggac
     3841 ggcgccttgg tcggtggcgc gagcttgaaa gcggatgaat ggaaaaaaat ggttgaagcg
     3901 attaaaacct tgcgctgaat gcgctgatgt tcttttgtag aacggcggga ataaaaacaa
     3961 attaataatt ataatgaaca aagacagcgt aaaaatatgg ttctgggttt tattcgatgt
     4021 tttcatctcc gtcgcgatcg tgaatgtggt tttcttcgtg atgcctgcgc tcgcgcaatg
     4081 gaaggattcg ttcacgccgg cgcgcaccat cacggttacg gcggaaggca aaacaacggc
     4141 aacgcccgat ctcgccgagc tttcgttctc ggtcgtttcg caggggaaga atccggcgga
     4201 tttggacgtg agtaataacg acaaaatgga tgcggtcatc gcgttcgtga aagggcaggg
     4261 gattgcgagc agcgatatcg cgacgacggc atacgacttg tcgccgaatt accagtacga
     4321 tgcaaacgca aacaggaatt atgtgacggg ctatacgttc acgcagacgg tttcggtgaa
     4381 agtccgcgac ttgacgaagg ttgcaagcat catcggcggg ttgacgccgc tcggcgtaaa
     4441 ccagatcggc ggcgttaatt tcacgttcgc cgatcaggaa aaattccttg cggtggcgcg
     4501 tgcggacgcg atagcgaagg cggaagcgaa agccgtctca atggcggccg atgtcggcgt
     4561 ttcgcttggc gcagtcgtga atgtcgggga gaacggaata gtgccgatat accagccccg
     4621 catggaagcg gcgaatacca tgatggcggt atcgtccgcc gcggtgccga cgatcgaacc
     4681 gggcacgcaa gacataacgg ataccgtgac aataacgtac gcgttgaaat aaattagaaa
     4741 ttttcattga taatccccgc cgttgatcgt agtcgagatc aggatccggc ccgcccgcca
     4801 aaatttttcg gaaaaaaact tgagcgggcg ggggcatacg cttcccattt cctcttttta
     4861 ttttcaaagc gcgggtcgtc cacggccaag ccggacgatg acagaaagag ggcggatgat
     4921 gacagagaga agggattacg gcggatgaat atcatcaaat ccatgaaacg aaacgatgtt
     4981 tatgcggcaa ccgcgatcgg cgcggcggtg gggcttttga tccagccgat ccttacgaac
     5041 ctttccagtt tcgcgatttt ggcgcacccg acggccgcgc tccggttggg gatattcgtt
     5101 ttcttcacgc tgttcgcgcc gcttgcgcta tggattcttt ttcttttcgg caagctggtg
     5161 ccggtagtat accagttcgg caaattcgcg gcggtcggca cgttgaatac attcgtcgat
     5221 atcggcgtat tcaatctcga aacattcctt tatgggacat ccgcgatcgg cattacgctc
     5281 ttcgcggtat tcaaggcgct gtcgtttctc tgcgggacga ccaacagttt tttctggaat
     5341 aaatattgga cgttctcctc ggcgcgtgcg acgcatcccg aaggcgccat caaggaaacg
     5401 aaagaggcgg tatcgttcta tattattgcc ggtgtcggct ggacgttgaa tgtcggctgt
     5461 gcgacgttcg tgaaatccgt cgagccgtcc ggcgtcgccg ttaatttatg ggtgaatgtc
     5521 gtcgcgccgc tcgcgggcgt cgccgcggcg ttcctttggg atttcgtcgg ctataaattt
     5581 ttcgtattca aagaaaagaa ataaccgcat tggttttttc gtagtatctt ttccgtcgtc
     5641 ctcgcggagg cgaggatcca cgctttctga atttaagacg ggaagcatgg attccctcct
     5701 tcgaggggac ggcagaaaaa gggaaagcga ggatgacgga atgaaatgac aaaagagcag
     5761 gtttgtgata tagtaaaacc atgccatcgc ttaaacaatc ccgcatcgta aaaaaaatca
     5821 tcgcgcatcc cgctgttttc ggggcatatc attttctgtg ggcgtggatg ggcgcaaaaa
     5881 tccaccggca tccgagcagg aaaatattcg tcatcgggat tacggggacc aagggaaaaa
     5941 cgaccaccat cgagctcctg aacgcaatcc ttgaggccgc cggaaaacgc acggcgctgc
     6001 tttcttcttt gcgcgtgaag atcggcgacg caagtgaaaa aaatatgtcc ggcaattcga
     6061 tgccgggccg cggttatctc cagagatttc tgcatgatgc cgtgcgcgcg cagtgcggct
     6121 acgcgatcgt cgaagtgacc tcgcagggcg tcgtcgcgca ccggcaccgt ttcattgact
     6181 ggaatatggg cgtaatcacc aatcttgcgc cggaacatat cgaatcgcac ggatcgttcg
     6241 aaaattatcg cgaagcgaag ctggattttt tgaaatacgt cctcaaaaaa ggcggcaagg
     6301 tttttttgaa tcgtgacgac cagcagttcg attttttcgc cgcagcgctt gcgggcatga
     6361 agaccgtcga atatgcgaag gcggatgaat cggcgatgcg ctatctcctg aaagcgtacc
     6421 cgatgcgcgc ggcgcaggac accgcgccgc cgaagtttat tttgagcgga ttcaataaag
     6481 agaacatcgc ggcggcggtt gcggtcgcga aagagcttgg catgggcgac tctattatag
     6541 agcaggcgct cgctcatttt gcgggtgtcg agggaaggat ggagttcgtg gcgtcggggc
     6601 attataccgc ggtggtggat tatgcgcata cgccggattc gcttgaagcg gcttatgcgg
     6661 cggcgagacc ggcgccgaac gcgcactttc cggaaccgaa gctgatctgt gttctgggtg
     6721 ccgcaggcgg tggtcgcgac aaatggaaac gtcccgcaat gggaacgatc gcggcgcgcc
     6781 attgcgacga aattgtcctg accgacgagg atccgtacga cgaggaccaa aaagaaattt
     6841 tggcggaaat aaaaaagggg attgatgaag cgggttttcc cgcggatcgc gtttatgaaa
     6901 tagcggaccg caaggaggcg atccggcgcg ccgtcggact gttgcgcgaa ggcgacgtag
     6961 cgatcggtac cggcaaagga agcgagggat ggatccgtct tgcggacggg aaaaaaatac
     7021 cgtggaacga aaaaaaggaa tttgaagaag cactcgctga aaaaagaagg gaaagataga
     7081 aggactattc gaaaactttc cttctgttat cgtctgaatt tctctgtcat catccggctc
     7141 cttttgtcat cgtccctgaa gagggacgac ccacgcttcc catcttctct tttttatttt
     7201 taaagtgtgg gttgtccggc caaaccggac gatgacggag aagagaaaga aaaaacagac
     7261 aattgcaatc tttcttttat agataaacgc cgattttttg atgaaaactg ataaaaaaat
     7321 aagccttgct cgataaatta acgagcaagg ctt
//
LOCUS       DAIDDT010000038         6653 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_38_length_6653_cov_5.184155, whole genome shotgun sequence.
ACCESSION   DAIDDT010000038 DAIDDT010000000
VERSION     DAIDDT010000038.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38_length_6653_cov_5.184155"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1667 a         1633 c         1748 g         1605 t
ORIGIN      
        1 ggcgtacact ttcctgtgta atacccctat gagcgcgagc tcgggggcgt ttggcacttg
       61 aaaggggtat gtcattatcc ggtttccttt gtcatcgttc ccgattattt tttaaagcgt
      121 gaatcatcga tcggaatcga gggtaacaga aaaaaataaa aaatcaaatg gcaaaacacg
      181 acaaaatcat catcaagggc gcgcgggaac ataatctcaa gaacatcaac ctcgagattc
      241 cgcgcgacaa aatgactgtt ttcactggtc tttcgggttc gggaaaatcg agtctggcat
      301 tcgacacgat tttcgccgaa ggccagcgcc gttatatcga atcattgtcc gcctacgcgc
      361 ggcaattttt gggacggctc gacaaacccg acgtggacga tattgaagga ttatcacccg
      421 caatctccat cgaccagaaa agccattcgt caaacccgcg ttccatcgtc gcgacgatca
      481 ccgaaattta cgattatctc cgcgtgcttt tcgcgcgcgc cggaaagccg tactgcccgc
      541 gctgcggccg cgagatcaaa aaactgacga acgaggaaat cgtggatgtc gttatcggca
      601 tctccgaaga aaatgacaaa gaaaacgccg cgggtgaaac cggatccgcg aaacgcaaaa
      661 tcgcgggaaa gaatcttatc atcctcgcgc cggtcgtgcg cggtagaaaa ggggagtatt
      721 accagatatt gtatgacttt ttgaaggcgg gattttccga ggtgcgcatt gacgggacgt
      781 ttcattctct ccgcgaccgc gtggaaattg accgctataa gcagcatacg attgagcttg
      841 tcgtggaccg tctgcctgcc ggtatggatt ttaaagaaaa agataatcgg ctgcggcttg
      901 cggaagcgat cgaatcggcg ctcaaatacg gcaataacgt ggtgaccgta atcacggaca
      961 aagaggtcgc gctgtccgcg cagttcacgt gcccgcatga cggtttctcg tttccggaga
     1021 tcgagccgcg gcttttttcg ttcaacagtc cttacggcgc atgtcaggaa tgccacggct
     1081 tgggtacgaa agacatttgg tccgatgaaa tctgcgaggt atgcgccggc aagcggctgc
     1141 gggaggaaag tctgaatgta ttgatagacg ggaaaaacat cacgggcgtt acggcgatgt
     1201 ccatcgagaa ttcttataaa ttcttttcga cgcttgcctt caaatcggca tccgatacgg
     1261 aagtcgctgt tgcgccgttg cgtgaaataa aaagccggtt gaaattcatg atcgacgtcg
     1321 gccttgaata tctgacgctt gatcgccgcg cgggaacgct ttccggcggc gaggcgcagc
     1381 ggatacggct cgcatcgcag atcggatcgc ggctggtcgg cacgctttat attctggacg
     1441 agccgacgat cgggctccat cagcgggaca acgcgaagct catcgcgacg cttcaggagt
     1501 tgcgcgatct cggcaatacc atcatcgtcg tcgaacatga cgaggatacc attcgcgcgt
     1561 cggatcatct tgtggacatc ggtcccgcgg ccggcgtgca cggcgggtat gtcgtagcgg
     1621 aaggccccat cccgcagata ttaaaagatc ctaaaataaa ttcgctgacg ctcgactatt
     1681 tgcgcgggac gaaatcgatc gccacgccgg aagtgcggcg taaggtgacg aaagatcagg
     1741 agcacttgaa gatccgcggc gcgtatgcga ataacctgaa ggacattaat gtcgacattc
     1801 cgctccggcg catggttgcc gtgacgggcg tttccggttc ggggaaaagc agcctggttt
     1861 atgacgtgct ctataaaaca ttagcggaca agttctatca cgcgccgcgc aaggccggcg
     1921 gaaacaaaac gatactcggc atggaatacg tgaaccgcgt gataaacatc gaccaggcgg
     1981 caatcggccg gacgccgcgt tccaatcccg cgacctatgt cgacgcatgg ggttatatcc
     2041 gcgatctttt cgccgcaacc gaaagcgcgc gtatccgcgg ctacaagccg ggcaggttca
     2101 gtttcaatgt ccgcggcggg cggtgcgaaa attgcgaagg gcacggcacg atcgcgatcg
     2161 agatgcattt tttgccgacc gtgtatgtca catgcgacgt ctgcaaggga aaacgcttcg
     2221 accgcgagac actcgaggta aaatattcgc cggacggctc ttcgaaaaag agcggtgcgg
     2281 ggaaaaatat ttacgaggtg cttaaaatga caatcgagga agcggtgaaa ttcttcggcg
     2341 acatcccgtg gctttacgac cggctttcga ttctggaaga agtcggtttg ggctacctgg
     2401 agcttggaca atcggcgacg accctgtcgg gcggcgaagc gcagcgcgtg aaacttgcgg
     2461 cggaactcgg caagcgtgat acgcgccgga cgctctatct tctggatgag ccgaccacgg
     2521 ggcttcattt cgacgacgtg cgccagctgc tcgtcgttct gcagcggctc gtcgatcgcg
     2581 gcaataccgt ggtagtgatt gagcacaacc ttgatgtcat aaagtccgcc gattgggtga
     2641 tagacctcgg ccccgaaggc ggagaaaaag gaggcagcgt catcgccgcc ggaacaccgg
     2701 aagagattgc gcggaaagcg ggaagtttca cgggggagta tctgaggaag gttttataaa
     2761 gatcgttgtt tatctgccgt cctcgctgag gcgagaaccc atgctttaaa aaaataaaaa
     2821 gagaagacgg aaagcgcggg tcgcccgcct tcacggacga tgacaataaa tttcatgcat
     2881 atctataaaa ctcttcccga aacgccgggc gtttatatta tgaaagacag ctttggccgc
     2941 atcctttatg tcggcaaggc gggaaatctc cgccggcgcg ttgcaagtta tttcgagcgc
     3001 ccgcatgacg cgcgcatcgc ggcgctcgtc gagaaaataa aaaatataga ataccggaag
     3061 accgatacgg cgcttgaagc gcttattctg gaatcaatgc ttatcaaaaa atattcgccg
     3121 ccgtttaacg tccgagaaaa agacgacaaa agttttttat acattgaaat caccaaagaa
     3181 aaatttccgc gcgtgctttt ggtgcgcgga aaagacaatg cgcgcggcgc gcggttcgga
     3241 ccgtttgttt ccgcgtccag cgcgcgcgcg gcattgaata ttttacggag gatttttccg
     3301 tggagcacgc acgatccggt gaaagtgatc gatccgggcg cgccaacggt ccgcgcatcg
     3361 aaaaccgcgc acggaacgcc gccggtcacg caggcgaagt ccgcaaaacc ggcgggacgg
     3421 ccgtgtcttg attatgagat cggcctttgt ccgggaacct gcatcggcgc gatcagccgc
     3481 cgggattatg tgaaaaacat cgcatggctc gaactttttt tcaagggaaa aaagaaaaaa
     3541 ataatagcgt cgcttaaaaa agaaatgacg gccgcaagca ggcagctcga attcgaaaag
     3601 gctgaaaaac tgcggcggca gctgttcgcg ctgcagcata tcatggatac ggcgctgata
     3661 ggggatggag aatctaaaat acggaattta gaatatagaa aaaatccgga atcaaacgaa
     3721 ccgaaatacc gcattgaagg ttacgatatt tcgaatattg cgggagatgc cgccgtcggt
     3781 tccatggtgg tttttgaaaa cggcgcgcca aacacggacg aatatcggaa attcaaaata
     3841 cggacgattt ttcagccgga cgatgccggt atgcttacgg aagttcttgc gcggcggttc
     3901 aagaataatt ggccgttgcc ggatcttgtt ttaatagacg ggggattgcc gcaggtgaat
     3961 gcggcgcggc gcgcgcttgt ccacgccggt gccaggattc ccgtcgtcgg catcgcgaaa
     4021 ggaccggaac ggaaacgcaa cgacatcatc ggcgccgtac cgaagggcgt ttccgaatcc
     4081 gtgctcgtcc gcatccgcga cgaggcgcac cgtttcgcca tcggttacca taaggcatta
     4141 agaagaagaa attttttgga taaccggaat aaataaatgg agaaaataca ggcagttatc
     4201 ttcgatatgg acggcgttat ttccgatact cagaaattgc acgcccaaat cgaatcggat
     4261 ttattgaaac aatacggcat tgctgaataa tttcgatatt atattacatt cagcaccgcc
     4321 cgcttatagg cttttgtaaa tcgatgccct gtgtctttta taatccttac tcctcctaat
     4381 ttaatcatcc tttcggtcat ttcttcatcg ccgcaaaaca tgataaattt ctttgctttt
     4441 ggttttccat agcgcgattt ctttaattca ttggttgttt tcctgccgaa ataatgaatc
     4501 atatcaccga ggattccttt cgcatcactt ccaagaatat cagacatcga acaataaatt
     4561 ccttttttaa cgggagtttt taatttataa gctatcaagg cgcccgttga aaatccaaaa
     4621 acaatcgagt ccgggtctat ttttgttttt gaaagcttta aaagagaatc accttttagt
     4681 tgtaaatcca agaattctac gtcgtatttt tgttttgcca tggaaataag ccaatgatat
     4741 ttgcgccgtg tttcgccgta tccgggaatg atataaaatt tcatattaat taaataaaaa
     4801 atagggggcc gctttggcgc acccccttac acttagagat cgaattctcc tttcttcacg
     4861 ccctgaatga aagcactcca ctcgtcccga gtgaacgaaa gggtcggact tagagaattt
     4921 ttggaatccc tgacgtccac cgtgtcgctt acccgaaccg caacgcaggt tttcatgggg
     4981 agaaacctgg tgattgacga agttttgaat ccttctgctt cgtgtggatt acgtagttct
     5041 ttttgagaag tattattgat cttcttcatt gaaagaatcc tccttttaaa gttaagtttg
     5101 ctattaacca tagcaatgga gatatgttta tttgtcaatt tttttattgg ttctacggca
     5161 ttttttatct acccgtttta cgccgatcat ctgctttcaa taatgtatcg gtatagtgca
     5221 cgatagtgcc ggcgcccatc atcacgagaa ctgtttttaa gccgccagtt tttatttttg
     5281 aacggccggc gtctgaaagg gaaatcaagg cgttcaatgc agtttttaat tttgccggat
     5341 cgcccagata agtgatagac ttgcccggct ggcgtttttg catcgcgcgg acgagcgctg
     5401 cggaatccag agaagacgcc tcatcgcggc cggcgacttt atagatggga agcatgacca
     5461 cgccgtcggc atcgtcgaat gccgattgaa attccttgaa aagcgccgta agccgccgcg
     5521 cctggtgcgg ctgaaagacg caaacgatgt tcgctttcga aaatttctcg cggaacccct
     5581 ggagcgccgc ggcgatctcc gccggatgat gcgcgtaatc gtcatagacg gaaaggttca
     5641 ttttgttttt tccatttccg atcttcaata cgccgcggta ttccattctc cgctccgcgc
     5701 cgtgatagcc gtgcagcgcg gcaaggacat ttttttccgc gaggccgagc gcgcggccca
     5761 tataatacgc cgcaagcgca ttcgaaagat tatgccgtcc ggaaatgatg attgccgcac
     5821 gcactttctt cgcggcagga tctcggagcg aataccagac gacgcgcaat tttttgcgtc
     5881 cggcaagcgc ggcaattttt gtcttcagcg aaaaacaatt tttgttatcg cggttgataa
     5941 ttaaaattcc gccatcgcgc atattcgcaa gaaacagtaa aaatgttttt ttgatatccg
     6001 cgagatcttt atagatatcg aggtgttcac ggtcgatgtt cgtgatgatg gcgctcgcag
     6061 gggagtaatg caaaaacgcg cggccaaact catcggcttc gaggacaaga tattcgcttt
     6121 gtccggcgcg gaaattccgt ccgccgtact cttttatgtt tgcgccgacg atgaccgtcg
     6181 gatcaaatcc gcccttttca agaaccagcg ccgtgagcgc ggtcgtcgtg cttttcccgt
     6241 gcgcgccggc aatggcgatg gtttcatgct ctcgcgtcaa ctcgccgacg gcctccggat
     6301 aggaaagcac gctcaggctt cggcccggtt gcgcgcggcg gcgcgcttct ttcaattccg
     6361 gattatcagg caggatcgcc tggctgtaaa ttacgagcgc cctatcggct gcgacgttgt
     6421 cttttttctg tcctattttt atcgtaatgc cttctttgcg cagcgccgca gtgatcgggg
     6481 attccgcgat atccgacccg gcaacgtccc atttctgaga taaaaaatag cgcgcaagcg
     6541 cgctcatgcc ggctccgccg ataccgatga aataaatttt attttttctt ttgggcataa
     6601 aaaataaaaa gagagaatac tataatttta taatttaaat gatatctctt acc
//
LOCUS       DAIDDT010000039         6272 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_39_length_6272_cov_6.789508, whole genome shotgun sequence.
ACCESSION   DAIDDT010000039 DAIDDT010000000
VERSION     DAIDDT010000039.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39_length_6272_cov_6.789508"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    6033..6042
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1298 a         1850 c         1470 g         1644 t
ORIGIN      
        1 aatcgtaacc cctcacgccc ctcttttccg tcgcccccga cccccgatca ggtcaagggc
       61 ggacgatgac agaaagagcg agaatgacag cggtgaactt taatgtcaga tgtccgcgca
      121 gcgcggacat ttgacatcgg aaatattatc gcttttttcc ttctataatc atctgcgcaa
      181 cattgctcgg cgtcggttcg tagtgatcga attccatcat gaatgaaccg cggccctgcg
      241 acatggaacg caattcggtc gcgtagccga acatttcgga aagcggcact ttggcgtcaa
      301 tcactttgag tcccggccgg tcgttgatgg cctcaatgcg gccgcgcttg gaattcacat
      361 tgccggtcac ctcgcccatg aaattctcgg ggacgatgac ctgtatattc atgatcggtt
      421 ccaatatcac gggcttcgcg cgcttcgccg cttcctggaa cgccatcgat cccgcaatct
      481 tgaacgccgc ttccgaagaa tcgacttcgt ggaacgaacc gtcgtaaacc gtcacttcga
      541 cgtcggtcat cgtatagccg gcgacgatgc ctttatcaag cgcttctttg acgcccttct
      601 ggatggccgg aataaattcc ctcggaatgg cgccgccttt aacttcgtcc aaaaacacga
      661 atcccgcgcc gcgctcgagc ggtttcacgc gcacgcgcgc atggccgtat tgcccgcgac
      721 cgccggattg tttgatgtat ttcccttcgc cttccgcttc gcccatgatc gtttcgcggt
      781 atgccacctg cggccggccg acattcgctt cgacgttgaa ctcgcgcttc atgcggtcga
      841 cgagcacttc caaatgcaat tcgcccatgc cggaaatgat cgtttcgccg gtttcgatat
      901 cgcccgcgac gcggaacgtc ggatcttctt cggcgaggcg gtgcaatgcg atgcccattt
      961 tctcctggtc ggctttcgtt ttcggctcaa tcctgatgcc gatgaccggc tcgggaaagg
     1021 tgatcttttc gagaataagc ggagcctcga tatcgcaaag cgtatccccc gtcgtcgtgt
     1081 ttttcaaacc gacgattgcc gcgatttcgc ccgcataaat ttcctccact tcctcgcggt
     1141 cgttcgcgtg catgcggacg acgcggccga tacgctcctg gttgtccttg accgaattca
     1201 aaacataaga accgcgcttg agaacaccgg aataattcct gaaatacgcg agcgtgccga
     1261 caaaaggatc ggtcgcgatc ttgaatgcca acgcggaaaa cggttcgttg tcggtcgcat
     1321 ggcgaaaaat ttccgcgccg gtcttcgggt catggccttt cacgggcggc agatccgtcg
     1381 gcgccggaag ataatcaacc acggcgtcca gcataaattg cacgcctttg ttctttaagg
     1441 ccgaaccgca catgaccggt acgaccttgt tcgcgatgac cccgacgcgg agcacgcgct
     1501 tcaggtcggc aagcgcgatc tctttgccct cgagatacat cgccatcagc gcatcgtcgt
     1561 tttcgacgat tttttcgatc agttcgtgcc gcgccttcgc gacctccgcc tgcatcgctt
     1621 cgggcaccgg caactcgatt attttttcgc cgtgctctcc ttcgaatccg tatgccttgt
     1681 tctcaagcaa atcgataacg cctttcagat ctgcctcgag tccgatggga agctggatgg
     1741 gaatcgcgtt cggcgtgagc cggtcaatga tggactggaa actcttcgta aagctcgcgc
     1801 ccatgcggtc aagcttattg atgaaacaaa agcgcggaac gttatattcg tccgcatacc
     1861 gccagttcgt ttcggactgc ggctcaacgc ccgcaacacc gtcgaaaaca acgaccgcgc
     1921 cgtccaggac gcgcagcgac cgctttacct cgacggtaaa atcaatatgt cccggcgtgt
     1981 cgataaggtt aatggaatgt tcgaacgcct tatctccttt gcgataggtc ggtatccaga
     2041 acgccgtcgt cgcagcggac gtgatcgtaa tgccgcgttc gcgttcctgt tccatccagt
     2101 ccatcaccgt gtcgccggta tgcacttcgc cgatcttgtg cgaaacgccg gtataaaaaa
     2161 gcacccgttc cgaaacagtc gttttgccgg cgtcaatatg cgcaatgatg ccgatattgc
     2221 gtattttttc tattggatat ttgcgtggca tattatgaaa aagtgaaaag tgaaaagttt
     2281 aaatccgctc ccctctgtcg tcctccgtga agacggaaga tccatgcttc caatcttaat
     2341 ttttaatcat taaagcgtgg atcctcgcct ccgcgaggac gacacgaaac gtgtcgccta
     2401 tcttgcgaag tgtgcgaacg cccgattcgc ttccgccatg cgatgcgtat cctgcttctt
     2461 ttttatcgcg gcgccctcgt tcttcgatgc ggcgatcaac tcgtccgcaa gcttgtatgc
     2521 catcggcttt ccttttttcg cgcgcgcggc ggcaaggatc catcgcaccg ccagcacgaa
     2581 ctgccgttcg ggccgcactt cgcgcggcac ctgatagttc gcgccgccga cccgctttga
     2641 cgcaacttca agaagcggcg atgcatgctc catcgctttc tcgaaaaccg cgatcggatc
     2701 ctctttggtc gtttctttta tcctgtccaa agcgtcatac atcacccgct cggcaacgga
     2761 tttttttccg tccttcatga tgtaattgat gaacctcccc acggcaacct tgccgtaaac
     2821 catatccgcc tgccgatttt ttttgtaaat atgtttctta cgcatgatga tgattattat
     2881 ttatgaataa attaacgctc ccctctgccg tccccgcgaa ggcgggaacc tatgcttccg
     2941 gtcttatttt tttaaagcgt gaatccgcgt ctccgcgggg atgacggaaa aacgctttta
     3001 cttcgctttc ggtctttttg ccccgtattt cgacctctgc tgttttcttc cttcaacgcc
     3061 cgtcgtatcc aaaacgccgc ggacgatgtg ataccgcacg cccggaagat ctttcacgcg
     3121 gccgccgcgg atcatcacaa tggagtgttc ctgcaagctg tgcttttcgc ccgggatata
     3181 tgccgtcacc tccatgccgt tcgttaaccg cacgcgcgca accttgcgca aagcggagtt
     3241 cggttttttc ggcgtcgtcg tgaaaacctt gaggcacacg ccgcgcttga acggcgaccc
     3301 cgaatcaacc ggccggttgt ggatcgaatt catatagtac gacaaagccg gcgccttggt
     3361 tttcgatttc aagggcttcc tgtttttctt tatcaattga tggatggtcg gcataatatg
     3421 cggcgcgcgg aacgtttatc gccccgagcg catattggaa aatactatgc gaaaaataat
     3481 ttcctgtcaa cgcccgctcg tggtggattt caaaaaaccc ttttcttctc tggctgaaac
     3541 gcacgagaaa acgcgaagcg tccaaaatat ttttaagctg aataaacttt ggtggaccca
     3601 ggcagaatcg gactgccatc tcctcattgc aaatgaggtg ttctaccatt taactatggg
     3661 cccgttatcg gaactcatct atatataccc tgatttaaat agattgaaaa gaggatttcg
     3721 gcgcgtgcgg atagccggta tggcttccgg aatgacaaaa gaaagcgggg cgacgcaccg
     3781 gcataataag aaaaaactct ataccgcatt ctccagtttc agcgacacga tctttgacgt
     3841 gccgtcttcg gcgagcgtca cgccgtagag gatctcggcg gcttccatcg tcgtgcggtt
     3901 gtgcgtaacg acgataaatt gcgtatgctt cgaaaaatcc ttgatcattc cggcgaagcg
     3961 gcgcgcattc cgttcgtcaa gcgcggcatc gatctcgtcc aaaacaagga acggcggcgg
     4021 actcacggaa accatggcga aaagcgcggc gatgccgatc aggctccgct cgccgcccga
     4081 aagcatgtca agcgacgtcg ctttcttgcg cggaagcttt atctctatcg cgacgccgcc
     4141 gtatgtctcc ggcgccgcct cttccccggc ttcctctttt tccgattcgg cctcgccttc
     4201 ctctccttct tcgcttttta ttttttcggc ttccggtttc aatactttca gctttgccgt
     4261 gccgccgccg aacatcacgc cgaaaaattc attgaacgcg tcgtttattt tataaagcga
     4321 cgcgtcgaat tccgtccgta tcttttcact caggtcgttc atgagccgca tgagatccgc
     4381 aaccgctttt tccaaatcct ccgattcttt tttcaaaaat tcgtaccgca cctcggtctc
     4441 gttcgcttcc ttcacgagca tttcatccac ctcgccgata ctggcaagat cgccgcgcaa
     4501 acggaaaatc ctccgttccg cttccgcaag cgaagcatgg ccgccgtccc cgtctacgaa
     4561 attcgcattg gcgtccgcca gtgcgaattc ttccatacgc cgccccgctt gttcaatctg
     4621 ccgcacaacc tcgtcgcgca taaggtcaag cctttctttt tcaagcgtat gttcgcggtt
     4681 ttgcttttcc cacagatcga ttttattttt cgccgtctgc acgccggcca tcgccgcctt
     4741 gaacgcgccg tagaacgctt cctggctctt ttcaagttcg gcctcttttg cgcggagcgc
     4801 cgtcaataat tcttcaagcc cgcgcaattc ttccgtgatc gcctcgaacc gcgtcttcac
     4861 gtcatccgaa acaacagccg gttttttttc ttccttaaca tgcagtacgc cgtcgatctt
     4921 ctcaaggatg gtttccactg ccgtctgaag ttccgtcata tctttatgca tcgccgccgc
     4981 gagttcgtgc cgtattttcc gcacgagatc caaaagcgcg tcggccggat agccgcccga
     5041 ttttttcacg ccggccgcct gcgccatttc catctgcgcc tccagccgcc cgagatcttt
     5101 ttgcagcgca ttccgttttt caaaaagctg gcgtgtctgt tccttcaccg ccgcaagatc
     5161 cttcttttct tccggctggt tcgcttcgac ctccgtttca cgcgcttccg cttcgcgcaa
     5221 ttcgctttca aagaccgcgc gagtctttgt gttttcagcg acaatcgctt cgaccgcttt
     5281 ccgtttttcc gagattgcgc ggagacggaa accgaaaaaa cgattctcaa gctcgcgcaa
     5341 ttccgcttcc atcgtttcac gtttttccca ccggctcgtc tgacgcttca gcgaccgcaa
     5401 gtgcggaaga atttcctcga taagcgcgcg cgctttatca agattgatgc ggctgttctt
     5461 gagccgccgc gcggcgtccg tcctttttat ctggtattcg cggaggccca gaatttcctc
     5521 gatcatttcg cggcgctcca tcggcgtcga ccgtataaaa agatcacttt ccccttgccc
     5581 gatgataatc atccccctgg aaccgaggcg cgccttcgcg aaaaaatcga taagatcgcg
     5641 gagcagtatt tcggatttat tgagaaaata tttactggtg ccgtcgcggc gcacttcgcg
     5701 cgacacggcg acttccttga aatcaaccgg aaaaaaatta ttggtgttct cgaaatgcag
     5761 acttgcctgt gccatgccga cacgcggccg tttctccgat ccggcgaaaa taaggtcttc
     5821 ggtctttcct ccgcggaggt tctttgcgtc acgttcgccc aaaagccacc ggagagcgtc
     5881 aacgatattc gatttcccgc ttccgttggg accgacgatc gcaacgatgc ccgccggaaa
     5941 ttccagcacg gtttttcccg cgaatgactt gaaaccgttc aattcgatgc gtttgaggaa
     6001 atgggacata tattaaacga aaagtgaaaa acnnnnnnnn nntgaaaaat tttaaattaa
     6061 taattttaaa atttctcact cataattttt cgttttaaat ttttcactat caacttcttc
     6121 acgcttttca cttttcatta ttcactattc atttttcaca ggctctcttc gtcgttctct
     6181 cgaaaaaagg ggtccatact tcccgtctta ttttctgaat tttaaatcat aaatcgtgca
     6241 tcgtaaaatt ccaaaagcgc ggaggcctgc cc
//
LOCUS       DAIDDT010000040         6227 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_40_length_6227_cov_6.239675, whole genome shotgun sequence.
ACCESSION   DAIDDT010000040 DAIDDT010000000
VERSION     DAIDDT010000040.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40_length_6227_cov_6.239675"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1576 a         1626 c         1474 g         1551 t
ORIGIN      
        1 atataaaacc gtcagaattt tttctatttt tctgatggtt tctttttgtt tcgaccttgt
       61 tttttttctt gaaaacggga aaaaaatgaa ataaggtgct tcaatctgaa tccctcgttc
      121 ttcacgtttt cgcgcgccgc gcggatatct tcccgtataa gatcggcctc ttcttttacc
      181 gctttgctta ctgcgcgcac gtcgcgcaaa actttaatga aataaacgag cgcaacggtg
      241 agcgccgcgg caacgacaat gaccgcaatc gtcgtcacga aaaaaaagat gtctgcatga
      301 accaacgttg tcatggaaat attatagcac aatcgattat tgttttgcac gcaaaaatca
      361 gcttcctgat aataaaaata aaaatgagcc tccggcgttg cgaaaaaatc gcggccgaag
      421 gctcaagggt tctaaaaaaa atgagatcag tcgaatttcc ggacggacgt gctcccgatt
      481 cgttgttcgc ggcgagtgtc cgaaacagca ccgatagaca gtccgttgga atcaatgtca
      541 ccgacgataa cacggactcg atccgaatcc acattcgaac agcgaacaaa tgttctcgca
      601 aatagctgcg gtgatttccg aaattcattg ccggctgttc atcaatcttt gcatcgcttt
      661 gtccttctcc gagaacggaa gttttgtttt tctgatcgcg gaaccgagga tggcgattgt
      721 ttcgtcgtat gttttccggt cgacgggata cggcgtcgcg tccttgccgc cgtgcgcaaa
      781 actgtagcgc gcgggatctt cgtatgaagg tttcgcgccg tagatgacct cgcctacgag
      841 cgcgagcgcg cgcacggttt tcggccccac gcctttcaat gacacgagcg actcgtaatt
      901 ttccggcttt gcatccgcaa cgaccgccag aaccttttcc agatatttac tcttcgaaaa
      961 atcctcatgc ataacgggat ggtatttgaa ttcccgcgtt tcaaggttca gaagcgtgaa
     1021 ctgttcgcca tgcggcattg cgatcgccgc cgactgcgaa agccgcgatg aatattttcg
     1081 gagcaacgta atatccttca tcagcgtcgc ataccctgcc tgcgcaagtt cggttgatat
     1141 ttcccgcgtt ttcaagcttg cccgcgccgt aaggtcgagc actttattga gcacgaccgg
     1201 cgacgaaatc cccgaatgcg gctcggaaac gaggtcattg atattttccg aataccagtg
     1261 ataacgccgc gccgaacgct tctcgcggtt cattccctgc tggacgaccg cccacgaacc
     1321 gccgcgcgag aaaaaaaacg cgtgatgata aagctggaac ccgtcctgca ccaaagcact
     1381 gtccactttc gcgctcattt tcgagttata gaccaatttt tccgcctgcg gtgcggataa
     1441 cgacagccgt tcgccccatg tccgtatttc gtccggcgtc tttcgcgacg tttttccctt
     1501 gccgccgcag ataaaaatac ctaaatcttt ttcctcgccg cgaatcgcct ccttcaatgc
     1561 cgctgtaaga attgtcgtca atcccgaagc gttccaatcg aacgcaagca cagtgccgag
     1621 cgactggaac catgccggat cggcaattct ttttataaat tcgtcgggcc cgtattccag
     1681 cacaagcgcg tgcgtcattt cacgtcccaa ttttaccatc cgctcgaaaa gccacggcgg
     1741 acaacgcccg ttatcaagcc cgaatgacgc aatgccgcga tggatcataa ctttattata
     1801 aactttccgc cgctatcctc aatgatctgc gtcgacgggg tgcggacacc ggaaacttga
     1861 cgccccttgc catccgatgc cctccgaaaa ttttggagtg gaaaaattta ggagggcttg
     1921 cattttttat gggctatggt atactcttcc cataacttaa tcgtaagccg gtagtagccc
     1981 tgagaggggc ttttttattg caaaatatga gtaaccatac atcggccata aaacttatct
     2041 caaaaagagt attttcgaag cggattttta tcctggcggt tgccacgtcg gcattctgcg
     2101 tcattcccgc cgccacaacc cgcgcggacg tgccgcttat agctgttccg agcgcatcca
     2161 cgtcgtcgcc ggccgcggcc tcgacaatgc ccgccgcagt gctgcatatg cggatcaccg
     2221 cctactcaag cacacccgac cagaccgaca gtactccttt cataacggcg aacggtacca
     2281 gagtccacga cggcgtcgtg gcgaccaatc ttttgccctt cggaacgaag atagagatac
     2341 catcgctttt cggcgataaa atattcaccg tggatgaccg gaccgcgcgc cgcgtaaaaa
     2401 acaccgtcga catctggatg tccagcacaa aacaggcgat cgatttcggc gtacactatg
     2461 cagatatcgt ggtcattagc cggcatggcg ccgtcggatc ggcgctcgcg atacaataga
     2521 aatttagtcg cccggatatt ttgggtcatg attgcgtgat tttctgacgg cgtatcgtta
     2581 gccggaaaat tgcgttggat gattaaatcc atcagcgtac gaacctctcc cgtccatctt
     2641 ttccgtcgtc ctcggtcatt aatcaggtcg aaaatctatg ctttctctgt cgtcatcgct
     2701 gaggcgagga tccacgcttt ctgaatttaa gattaggagc atggattccc tccttcgagg
     2761 agacttacgg agaggaaata tctcgatgga atcagtgctt gattatggac agtgagatgt
     2821 taccagccgt gcgcattgac aatatttcat tataagtctt aaattaccgt gataggaaat
     2881 tgttcttcat aacttaataa taaaaggagg ttgtgatgat tgccacaaaa gaaacatcga
     2941 aaaaagaagt gcctaattgt ttttgcgaag aagaattatg gtttgagttc aaaagcgatg
     3001 attttcgggc gcggcgcgag caatggaatt tcgaccggca gtgcgaacgc gcgttacttg
     3061 caaaaggagt caacgaccac actatcccga cagatgccgg acagatatcg ctcgtgattg
     3121 tcggcgcgat ttccctcgcc ttcaccggaa aatgctatat cgatttcaat agtatagcgg
     3181 gcaatgataa gtttgatatc gcgtggaatg ctctttggcg tacaaccgaa gatcatttcc
     3241 gacaacgggc aatacaaaaa gagaaatggc aaagtttcga ggcagcaatg cttcgtaatt
     3301 tcctaccccg gcattatcat acgccgccgg aacgacctta ctacgagagt agattcgagg
     3361 aaagatggaa cataaattat attgacagca tggattccat cggcaacctc cgtatttcgc
     3421 ttattaagca gggattgaac gactttaaat atctaccaaa gaaaattcgc tctttgattc
     3481 gcaagtcttc gacctgcatg caaccgctgc ttaataaatc cgcacaacag ttacagttat
     3541 agagaacaag aaaaacatac cccgttcaag atactattct tgcgcggggc tttttattga
     3601 tcgcgctcgc caccggtgtc gcccgcaccg tccgccgcgg tgcggatctg tccgtgccac
     3661 ccgccacgac ggcgaaactt tttccttttc ccgaaaaaaa tccgtcgcgt tcaagacctg
     3721 cgatgatttt ttccagccgt tcccgcggct gttttaattc ccgcacgaga agatcggtgg
     3781 aaactttttt ccgcgcaagg acgatgcgga gaattttacc gcgcaactcc ctgtccgatc
     3841 cggaaaattt cggctgccgt ttgtaatgcg cgctccgcat attcggattt tccgtccctt
     3901 tctttatggc gcgttcgccc agcatcgcgc cataatccat gagcgcccag taccattcgc
     3961 gcggattttc gcggtcgacc gtgcgcgcca cgatggccgc tatcgacgcg tcacgcacgt
     4021 tcttctttcc cggaaaaaag aaatggatga acacgcggcg gatgttcgtt tctatgaacg
     4081 gttccggcgc gttgaacgcg aacgccatga tggaaccggc ggttgccgtt cctattcccg
     4141 gcagcgattc gagaacagcg cgatggcacg gcaggcagcc gtaatatttt tcgacgaccg
     4201 cgccggcgag ccgccaaagc gaaagcgcgc ggcgattata accgagcccg gcccatgcct
     4261 gcaggacatc cgttgcatcc gcgcgcgcga gcgttttgaa atcgggaaaa cggcgcacga
     4321 acggttcgta atatttttcc acgcgcgcga cctgcgtctg ctgcagcata atttccgaaa
     4381 ccgttatccg gtatggatcc ttagttttcc gccacggcat tgaccgccca tgccggcggt
     4441 aataatccat gatgattctc cggaattgct tgtcggacat ctgcacaata tatcacgcat
     4501 taaaaaatcc cgcgatattt ttaacggttc cgaaatacta atacgaaaaa aaaacgactc
     4561 cgaaaagcag tccaatgtca agtacttttt tccgctacag aagatataca gaggataaca
     4621 acgcaaagcc cgtctcaaag atagattcta taaaggatgt ctctgagaaa agctttttaa
     4681 atgagtaaaa atgtgcactt gtcgctgtta ttcaatctgt tatctatccg attcttttga
     4741 aaatttcggc aatccccgct aaccttatct tgagacgggt tcaagaaact tggcgtagca
     4801 gcggtgaatg tgatatacta agccatagtt ataaaagtcc ttattaccga gaggaggtta
     4861 gtcgcctttt tctctaatgt tcgtgataaa ggtcgataat gctcagtcat aaaaaaatta
     4921 atacaactca tcagagttaa taaaatacaa tgaaaatcaa taaaagtgca ataactatat
     4981 atatatatat atatatagtt gcaatcgtcg cttcagttgc acttggtctt accggatcga
     5041 ctaccgcgct tgcggcggcg ggtccggcaa cggtaaatct cggatctgcg ggtaattttg
     5101 cgattttagc aaaaacagga atttcaacca cgggatccac ctcgattatc ggaaatatcg
     5161 gcgttagtcc ggcggccgcg agctacataa ccggatttgg attgataatg gacgcttcaa
     5221 atacgttttc gacatcggct cttgttaccg gaaaaatata tgcggcggat tacgcggttc
     5281 ctactccgac cacgatgacg acagccgtca gtgatatgga aactgcctat accgatgccg
     5341 ccggaaggac gaatcctacc gagaccgaaa tgggcgccgg aaatatcagt ggaacaacgc
     5401 ttgttcccgg cctctataaa tggagtactg acgtgacaat tcccaccgac atgaccctct
     5461 cgggcggccc gaatgatgtc tggatatttc agatagcggg aaatcttgat atcagctccg
     5521 ccgcgaaaat aatccttgcc ggtggtgcgc aggcaagtaa tgttttctgg gttgttgcag
     5581 gcaagacaac tatcggcaca acggcagtat tcaatggaaa tatattggat cagacaacca
     5641 tcgtactgaa taccggcgca acgctcaacg gtagagcact tgcgcaggcc gcggttacat
     5701 tgaactcgaa cgccgttacc gtgccagctt caacggaatc ggcatctgtg aattccgcaa
     5761 ctccggcaac gccggcagtt tccgcaatcc caccgtcagg gacaacaagt acttcggcta
     5821 ctcccgcaac atctgcggtt cctgcgacgc ccagcgcgaa cgctacgttg caagcccagg
     5881 ttagcgtatt attagcgacc ttgcattcgc ttcaggcgca gtatgccgcg cggcagcaag
     5941 gttccgtttc cgcccatgct tcatcttccg ctttcggcca gcaagtgcgg gttattgcaa
     6001 tgaatcttgg aaaaggaagc catggtagcg atgtaaaaac cctacagcaa tttttgattt
     6061 ctcagaataa aggtccggcc gctcaggcct tggctcacgt tggagcaacc gcttactttg
     6121 gagctctgac tcgcgcggca ttggccgagt ttcaggcgag cgcagggata agtccggctc
     6181 tcggaaattt cggagctata acacgggctt atctcagtgc gcattat
//
LOCUS       DAIDDT010000041         6185 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_41_length_6185_cov_7.544695, whole genome shotgun sequence.
ACCESSION   DAIDDT010000041 DAIDDT010000000
VERSION     DAIDDT010000041.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41_length_6185_cov_7.544695"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    489..498
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1540 a         1715 c         1432 g         1488 t
ORIGIN      
        1 ccgcctgctg atcggacggc tgatccgcaa aagaaaaacc atccggcaat atgagctcga
       61 gcgctccgtt cttaatgatg gtctccgaat tattggataa cgaaacggaa atgacgaacg
      121 gctctccgat aaaaacctgg tcgggttttg cgaatgcaac gccgacatcc acgttcggcg
      181 tccgcagaaa aacgaaataa acaaccccga cggcggtaag gacggcgaga acggcgaaga
      241 accacaacat gctgtttcct tttccgccgc cgccgtcgcc gctcggcgaa acgccggaaa
      301 tcccctcctc caccgcgggg gattccgtcg aagagccgta ttgtttctgc ctgctcgtcc
      361 aaattgaatt cggattataa tccgccatcc tttaataata tcccatttcc gcccgatctt
      421 gaagaacccg cccgccgcca gtaatctctc acgcccctct tttccgtcgt cctcgccctg
      481 tggagtagnn nnnnnnnnaa gccgccactc cacagggcga ggatccgcgc tttgaaatct
      541 tcggatcggg agcatggatc ccatccttcg ggggacgact gatggaaaat gtttcgacgg
      601 aatcggcgct ggttatagac agtgaggggt tagccgtcat ggttgctttt ttttagtttt
      661 aatgtattct atattcccac ggggacgcac tggcttcgat agcgatgcgc cgcgggtttg
      721 cgggctgatg gcaacggcat aatcgttgca ccaacaacaa ctgccaacaa caaagttgca
      781 gctcccgcgt tcgcttacgc gtaatgcggg gatcggctgg ttcgggcttc ttgaaccaat
      841 caccggtctt atataaggaa gctggcgcgc ggatgatgat tctacgccgc gcgcgagaac
      901 attgaatcgc attcgtttcg acttcggtca aactacgccc gtagacggcc caccggcacc
      961 atcgttagac gagagttcga ttctctccgt ctccacagtt cagaattttc cacaactgcc
     1021 gcggctacgc cgcggttttg tgtacgccaa ttgtatagat aatcaaacgg attttttaac
     1081 gcgagagtga aaagagaatt ttgtattttc aggtttgacc cgacatctcg gagaaaatca
     1141 cgcatagagg cataattttc tccgacacat atggtttctg cggtactttc ctgccaaaat
     1201 ccagtttcct tcacgacgga tggaaatatg gactatgaaa atggtataat gaatccataa
     1261 gcgccaaagg tcggagaaaa accgtacaaa ataaccatgg aagaaaaaat gaatatgcaa
     1321 ggcaagacat gcaagtgtgg ccaccatatg gcaaagcccg tgcttttgct tctcatagga
     1381 ctcgatatcc ttctgggagc actcggcgtt ttcacaagct atttcgtgca ggtcacatgg
     1441 ccgatccttc ttatcatcat cgcggtcatg atgatgacgg ggagacattg caagtgcgac
     1501 caggcgaagt aggtcgaaaa atcttttgcc gcaacgcaga aagaaaagaa tcgaatgaaa
     1561 caaataaaac ccctgctcac ggcaggggtt ttatttgtag ggcgtgcgca tttgaaaaaa
     1621 ataataagaa aatgcctcaa accggcacaa gcgcatacgt cctatcttgt ttgaaattta
     1681 aaaaaatgtc ccgtgaattt cattactcgc agcgtcgaag catgaaagaa aaatggcggg
     1741 atatttttcc gtcttttact tttcctcctt cactgtttcg acattcgccc gccgcagcag
     1801 ttccgcctgg agccgctccg cgagcgccga atcaatcgcc ggcaaaatgc cttccgattc
     1861 gttactcgcc tctttttcat cctcgcggcc tgcagtcaaa ataatgagcc ggcttacacc
     1921 aagcatccga aaagaaaaat cctgctcgat gtcgacgtcc tggatctgcc ggtacggaat
     1981 ggcatgatct tcgcgcgcaa taattccccg ctttatcttc agcgcatcct cgtcaagcaa
     2041 aaacacgtaa tttatataaa tgagccagct tatgaaaagc gcgaaaagga aaatgacaac
     2101 gcacagaccg agcgcgcccc atgcggcgag cgcggcataa cggctcatgt cgccgaacgg
     2161 cgtcgcttta agaaaaggca tcccctgtaa gacgaacaat aaaacagcga ttccgagaag
     2221 cgccatcgcc gcctgcgatc gttcaaaaac aaaaagccag aacgtttttc tgcccagctt
     2281 ttcgtatgtt cctgcggcgg ccggcgtttg tttttggata tcctcgtcca tggaattcat
     2341 tatagcataa ccatagtatc tttgatattc attcatggag cgttttctct ttccgcaaat
     2401 acttttatcg gaatgatcgt tccgataaaa gtatttcaag gcatccgacg tccataaaaa
     2461 ttctcatctc tttgacaacc gctcactttc ctttacaata agcatatatt tccatagata
     2521 tagcgtaact tttattttac ttccatacct aagccgagga tcaacaacca aataaccgct
     2581 cccgaactgc gcgtcatcga cgagaaagga aataatctcg gcgttttgaa gcgcgaggat
     2641 gcgatcctgc tcgcaaaacc ggatgaaggc atcgatctca tcgaaatatc ggcgaatgcc
     2701 gtgccgcccg ttgcacgcat tatgagctac gacaagtacc gctacgagga ggaaaaacgc
     2761 gaaaaaaagg aacggcttgc ccagaaaggc gcggcggcga aacaagtaca gatcagcgcg
     2821 cgggcggcgc ggaacgatct gcttctgaaa gcgaaacagg cggaaaaatt cctcaacgaa
     2881 ggacgcaacg tggaaatcgc catgcgtctc cgcgggcgcg aaaaatataa ccagccgtgg
     2941 gcgcggcaga agcttgatga atttctcgcc atgattccgg ttgacttcaa acgcatcacc
     3001 gaaccccgct tcggtggacg cggtatgaac atgcagatca cgaaaaaata atgtctgatg
     3061 cggcttcctg attcaaattc aaagtcggct tcttaaaatc cgcctcaaaa accgtcactt
     3121 atccgcgcgg aaaaaaacaa acggataaaa actgtttgat gcgaacgcta taaatattcc
     3181 gctccgattt cgcgcttcgg gacaagcgcc ggatcggtgg gaatagtgaa cgtgactatc
     3241 gtgccgcggt ccacttccga cgcgacagtc atcgcgccgc catgcgcggc gacaatgctt
     3301 ttcgcgatat aaagaccgag tcccgatcct tcggtctgcg atttcacggc attgtcggcg
     3361 cggaaaaatt tcgtaaaaac cttttccatt gattcggcgg gaatgccgat gccggtatcc
     3421 cgcacgctta ccgcaatgaa aggcgcatca tccttctttt ccgcttttac gatcacttcc
     3481 ccgttcgcaa cgttatagcg gatagcattc tctatgatat tgacgacggc gatggaaagc
     3541 tgcgccggat cgatcataac gcgcggcaac tcatccgaag gacggtcaaa atatattttt
     3601 atgcccgcca tccgcgcctg cggcaccaca tcgcccagga tgttccctat gaaatcaacg
     3661 atgttcgctt cctcgaaatt atagcccaca tgcccttcgt ccattttcgc gacaatcaaa
     3721 agctcatcaa tcctgcggag aaggtcgcgc gccgcaccgg aggcgcctgc caccataacc
     3781 ttgtccgtat cattcagtcc ggttgattgc gcaagcgact ggaacgccca gttgatattg
     3841 gtcaacggac cgcgcaattg atgcgaagct accgtcacga actcggtatt ggcgcgcaac
     3901 gccgcgattt cccccgtgcg atcacggatg atcttcataa atgcaagcgt tgcgccgcgc
     3961 tcttcggtga tcggcgccgt cgtaacgcgc agttcgatcg cgggatcgct aaaagaaaga
     4021 tccaccacct cgggatattc cccttctttg gatcggctca cgacacgcgg cgcaagcgaa
     4081 gaaaaaataa cctgggtaag cgtcgtccaa cccgcccgtt ccacgtcctg cggcgtgaat
     4141 gtatgcccta tcacctcctt cgcatttatt ttgaaaagcc gctccgccgc aggattgaaa
     4201 aaagtggcgt taaaatgatt gtcgtaaacg acaagcgcat cttcaagccc gctcaagaca
     4261 ctttgcaatt cattccgctc gatcgcggtc atttgattgg ttcgcgccga cgcatacaca
     4321 cccataaaaa agaggatgaa aacgacgcat aaaaatccag cttccaacaa ggcgaaaagc
     4381 ggcgacagga acgatatgcc gattgcgcat acagcgataa tgagcgcaag cgatacccat
     4441 accgttacga actcgcgccg cgcgatgata tttttgaatt gcgatgattt ttttttcatt
     4501 attgtttttt atgcagcgaa aaaaaataat cttatatctt ccgtatcccg atccacgatt
     4561 ccgcgccttc cattttgatt gtttcctccg cattcatctt atcagatcgc ccgatattta
     4621 tcatttttgc ggcgatctcg gttttcacga acgcctcgtt tttcgtgatc gcatcgcgca
     4681 ccggcggcgg aacatcaaac gcaacggcga taatatcttt cggctgcagc gccgccttct
     4741 gccgcaattc ctgcaccttc cggataaatt cgcgcatcat tccctcttcg cgcagcgcgg
     4801 gcgtgatcgc ggtatcaagt tcgacgtcat cttttatctt cgcatcgaaa acgatttctt
     4861 tcacattcac ctcgtcggca agaatgtcaa gaaattcttt ccctattttt aacttcccgc
     4921 ttttttgaag cgtggatcct cgccttcgcg aggacgacat aagaatgtcg cttttaactt
     4981 ttagccttgc cagcggctgg cgcactttta ttcccgccat ggcgcgcgcg gaaagcccta
     5041 aagccgccgt ctcacgcact gcctgcatcg acgccgttaa ttttctgccg cttatttcct
     5101 gtcgcttatc gcctgatgca ggatattcgt ccaaatgcac cgattctttt tctccgccaa
     5161 gccccgcata cagcatctcc gcgaaaaacg gcgtgaacgg tgccatgagt ttcgcaagcc
     5221 ccaaaaggac atagtgcagg gtcgcggatg ctgcctgata atcggcggca gtttccggtt
     5281 tctgcaaacg ccgccgcgag cggcggatat accaccgcga aagatcgtcg atcaaaatcc
     5341 ccgtctcaag cgccgcttcg cggatctcat aacgttcgag tttttgcgcg acgccgttta
     5401 ttgtttcatg aagccgcgca agaatccatc gatcgagaag atttttgctt tttaccgcct
     5461 ttgattggcc ggccgtcctg cgcgcgtacg ttttccagaa aataaacgaa tggtaaacga
     5521 tgaggtgcgt tttccggaac gatttcataa tctcctgctc gtcaaaatcc ttcggttcgc
     5581 cgagcggcgc cgccgtaaag aaataccagc gcacggcgtc aatgccatat ttttccatca
     5641 tatcccatgg attgacgata tttcctttcg acttggacat tttctgcccg aacttgtcat
     5701 tgataagacc gagagaaacg acattcctgt acggcgcggg ataatccatg gcggtcgcaa
     5761 tcgcaagcat ggtataaaac catccgcgcg tctggtccat cccctctgcg atataatcgg
     5821 cggggaaatc caatcctgcc cgatgctccg tttttgattt tccgtttttg attcgaacgg
     5881 acgcgaacgg aaaatgcgcc tgcgcaaacg gcatagcgcc ggaatcgtac cacacatccg
     5941 ccacttccgg cacgcggcgc atatccgccc cgcatttcac gcacggcacc gtcatctcgt
     6001 cggcatacgg ccgatggaga tcaacctcgc cgctttcgtt gcgcggcaga attttcacgt
     6061 caagcttccg gatttcggcg aaatctataa atacaccgcc tttcccctct tcgtctattt
     6121 tttctttttc cgtgatcgcg cgtttttcgc tccacccctc cgcggcgtgc gacagcatcc
     6181 agatc
//
LOCUS       DAIDDT010000042         6050 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_42_length_6050_cov_4.920141, whole genome shotgun sequence.
ACCESSION   DAIDDT010000042 DAIDDT010000000
VERSION     DAIDDT010000042.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42_length_6050_cov_4.920141"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1288 a         1720 c         1306 g         1736 t
ORIGIN      
        1 gcgcgggagc gtcctcacgt gcaattcccg tatctcggcg aacccgacgt actcgccctt
       61 ctgtgcctcc ttttccgcga tggcgcgctt ctcactccat ccttcggcgg cctggaaaag
      121 catccagatc ggatattcat ggctcacgat gagaatattt ttttctttat attttttctc
      181 gcaatcccgc aaaaattccc acacgcggcg gcgcacgtcg cgcaaagatt caccgccttc
      241 gggccgtttt tcaaaacgcg attcgtaggt cgagaactgt ttgtgatatt tctcgtcgcg
      301 atagccgtca aacgccggcc ctaagtggat ttcttcaagc cgcgcgtcga taagaatttt
      361 ttctcccgta aaaatttccg cggctatatt tcctgtctct ttcgtccgca gcacgtccga
      421 tgcgataata atgtcgatat gcttatgttc ccgcgcaagc gacgtcttca atttctttat
      481 cgccgccgta acctgtcttt ttccgcgcgg cgtcaaatga aacgcgccgc ctttcgaatt
      541 gatgatatca aaagcattat tttccgcctc gccatgccgc atcacccaat aattattttt
      601 cggtccgccg gcaagacggc ttagttcatc catacttccc gcgaccgacg catgcccgca
      661 ctttttacat tcccagatcg ggagcggcgt gccccaataa cgttcgcgtg aaagattcca
      721 gtcttttgcc tcgcgaagcc attcgccgaa cctgccttct ttcacgtgcg ccggcatcca
      781 gttgatattt ttattccgcg ccagaagttc tttccgaagt ccgctcatcc cgataaacca
      841 cgatattctg gcgtaatata aaatcgccgt gccgcaacgc cagcaatacg gatattcatg
      901 cgcatatttt tcaatactca gaaaattgcc gcgcgctttc aagtggtcga aaattttttc
      961 ttccgtttct tttgctttcg cataccggcc ttcaagctcc ggaacgtcct tcgtgaattt
     1021 tccttcttcg cttacggtat ggcgttccgg aaacccgaat ttttttccaa gcgcgtagtc
     1081 atcctcaccg tacatcggcg caatatgcac gacgcccgtg ccgtccgtcg tcgtcacgaa
     1141 atcagcggga tagattttat aagatttttc attcttcaac gaaggaacct taaaaagcgg
     1201 ctcgtattcc aaacgaacga gatcggaacc ttttatctct tgaaaattaa aaattcctaa
     1261 aaattcattt gcatttttat cccaaccgga acgctgatcc gtaatacgac tatcctggtc
     1321 atctcttgtt ctctttatga atatatcttt cgcaagaata tatttttcgc gggaatcaat
     1381 cttttccgcc acaacatatt caatgtcttt ccccaccgcc aaagccacgt ttcccggcaa
     1441 tgtccatggc gtcgtcgtcc atgacaaaat atatgtttta tcatccgcca taaaaggccc
     1501 gattttctgt ccggacttca atttgaattt cacatacgcc gcattgtcct ccacctcctt
     1561 atagccttgc gcaagctcat gcgaggaaag cgccgtaccg cagcgcgtgc accacggcac
     1621 cactttgtgc cccttgtaca ataatttgcg ctttgcgatc tgtgctattg tccaccaaac
     1681 gctctctata tattccggct cgtaggtaac gtaagcattt ttaaggtcca gccaatagcc
     1741 gatgcgccgc gtgatatttt cccattcgtc cttataaagc cacacctgct ccttcgcttt
     1801 tttattgaac gcctcgatgc cgtattgttc aatctctttt ttcgacttga aacccagcgc
     1861 tttctccgcc gccatctcga ccggcaagcc gtgcgtgtcc cagcccgcgc gccgcggcac
     1921 gtaataaccg cgcatcgtct tgtagcgcaa aataacatcc ttcacgacgc gcgcaagcac
     1981 gtgatgaata ccgggcttcc cgttcgcata cggcggccct tcgtaaaaaa tgaatttctt
     2041 cttcgccgcc gcggacgtgc gttttgataa attcttttcc aggcttttct caaaaatttt
     2101 atgctcgtcc caaaatgcca aaactttttc ttccagtttc ggaaactcga attccccgtc
     2161 aaggaaattc gggacatgat gcgccggagc gttctttgtc ttcctcgttg tttttttcgc
     2221 tgcgcttttt ctggtttctt tctttgccgg cattctctaa aaataccaga tttttcaacg
     2281 aatctcaaca ttatcgagat tctgcctcga taatgttttc ttcgggacgt acgcgcttgt
     2341 gccgagtacg agacattttc cggttatttt ccgggacgta gtccgacggg tgctcggcga
     2401 taaaaataac cggaaaataa cggaggaacg ggccttgtcc gatgcggttt gagatatttt
     2461 cttatgattt tttttaaatg cgcatgtcct attcttaaat cttttccggc gcctggatac
     2521 ccaagagacc gaggccggtt ttcaggacgc gggcggcggt ttcaataagc gtgagccgcg
     2581 cctggcggcg ggattcgttc tcgtctttca atatcggcgt cgtttcatag aatttgttcg
     2641 cgacggacgc aaacttgtat aaataatccg cgagcggact cgtcatgaga agctcgtttg
     2701 attttttgac ggcatcggga aattcgaaca gcttccgcat tagcgcaagc gccgcatcgc
     2761 cgtcaagcgc cgcaaagtcg cgtcccgcgt ccttcgcgtc ccgttccgcg tatcctgatt
     2821 ttcttaaaat actccgcatc ctcgcatagg tatattgcag atacggtccg ctgtcgcccg
     2881 tgaacgcaag catccgttcc caattaaaaa cgatatccgt catccggttc tctttgaggt
     2941 cgttatattt cacggcgccg atgccgaccg cttccgcaat ctcgtttttt tctttttccg
     3001 aaagttccgc gttctttttt tgaaccgcct ctttcgcaag ggcgaccgcc ttcccgagaa
     3061 tctcgttcat ggaaaccgtg cggccctccc tcgtcgagag tttcttcccg ctctcgccgc
     3121 gcacaagacc gtatttcacg tgccgcagtt cggcggaatc cagtttcagc acttttgcga
     3181 cggcaaaaag ctgctcgaac tggaacgact gctcattgcc gatcacatat aatatcttcg
     3241 ccggcttata ttttttgagc cgatagcgga gattcgcaat atcacgcgtg agatataaac
     3301 tcgcgccatc ggacttctcg ataagcgccg gcggcagatt gaacgcatca agcttgatca
     3361 ccatcgcgcc ctcgctgcgt tcggcaatgc cgcgctcgca caattcccgc acaaccggct
     3421 tcatttcgtt ttcaaaaaaa ctttcgcctg tccatacgtc gaaatcgaca ccgagcgctt
     3481 tataagaccg cttgagctcc tttatcgact cttccctgaa ccacttccat aatttccggt
     3541 tttccttgtc gccgccttcg agttttttga attcttcctg cccgcgcctt tcgagctccg
     3601 gatccgattt cgtttcttcg tggaaacgga catacagctt ctgcaatgct tcgatgggat
     3661 tcttcattac cgctttttta tcgccccatt gccggtacgc cgcgataagt ttcccgaact
     3721 gcgttcccca atcgccgaga taattccagc ggatcacggt ataccccgaa aactcgaaaa
     3781 tattggcgag cgattcgccg atgatcgtgt tccgtaaatg ccccacattc aaaatttttg
     3841 cgatgttcgg cgaggaatat tccacaatca ccgttttctt ttttccgctc tcgctcctgc
     3901 cgtaattttt atccgaaaaa acttttttaa attcttcctg cagcgttttt ttgctcaacc
     3961 agaaattaac gaacccggga gcggcggcct cgattttttc aaaaaaaccg gcgggagcgg
     4021 cggcctcgat tttcacggca agctcttgcg caaccgccat cggcgttgcg cccgcttttt
     4081 ttgcgccttt aacggacgca agccgcatcg cgacgttcgt cgagtaatgg ccgaatgttt
     4141 cctgttccgg cacggaaacg tcaaccggcg taccggcagg cagtaatgat cttaaaatcg
     4201 taagaatgga atctttcatg ggggaaaaaa attttagttt tgccgcctcc tcttgtcatc
     4261 gtccgcgaag acggacgacc cgcgcttccc gtctttttta aaatttttaa agcatgcgtt
     4321 cccggctcca ggcgaagatg acagaaaatt cgaaacgctc tatcccgtca gcgcctcacg
     4381 gagcattatg agtccccggc gatggcggct tttcacggta tcgatcggct cgcagagctt
     4441 ttccgcgatc tcgatgaact taagattttc gttataacgc agctcaaaaa cggcccgatg
     4501 caatgccgga agtttatcaa gcgcctttcc gatgccggtt ttgaggatct tttggtctat
     4561 tatgcgatcc agcgacacgc tttcttcggc atagggcgca agtaaagacg ccagcgcgtc
     4621 gtcgctttcc gccatacgcg aaaaaaacag cggccttttc ttttttatgg aatcaagcgc
     4681 ggcgtttttc gcaatcgcaa aaagccatgt tttgaatttt ttttcccggt cgaatttttt
     4741 caggtgtttc cacgcgcgca tgaacgtttc ctgcgcgata tcctctgcct ccgccgcatc
     4801 gcggacgtaa gaataagcga atttataaac ggcatccacg tggcactcga caagaatcgc
     4861 aaaagcgccg gcatcgcccg caagcgaatc tgtgataatt tcgatttccg tttttttaat
     4921 ttctttaaat gacatgtcta tatttacgat cgtttatgaa atttattctt ctctgtcgtc
     4981 ctcgactccg gttaaggacg gcccaataag aattgggccg cttttcactt ttcgtttatt
     5041 cttttatacg ttcccataag ttcggtttcc gcaagaagat atttttcgat ttcttttttc
     5101 acccgcgccg ccgaagatcc ggcgggaaga tcaagcgtgt ttgcgagcgc gtataaataa
     5161 aaaatatagc gatgtgttcc tttatgcggg cacggcgcta cgtaatgcgc gtttccgccg
     5221 ctcgtcgctc cttcggcgct tcccgccgga acgctctccg ctttgatgac cgccgttgcg
     5281 ggattgatat tccacaccac ccagtgcgta aaaccgcccg gcatcggcgc atcggggtcg
     5341 tgcataatga gtgccaaact tttcgcccca tccggaacat tctggatctg gagctccgga
     5401 ttgatgccgc ctccgtcgca cgtatatcgc acgggtatcg cgccgccatt ttcaaaatga
     5461 ggactggtaa ggatcataat tctcattatg ccacattttt attatttttt ccactctctg
     5521 tatgaggccg cccgcttttg cgctctccat cgttattgtt tatcacctcc atcaacgcgt
     5581 tgtgcggtaa acgaccggta ttcttcgcgc atcttcgcaa gcgcggtttc gctttttggc
     5641 ctcgttcttc ccggatagcg ccacgcgctg atgatcgtcc ggcgatcgtg cgaatcctcc
     5701 accatcaccc agatttcctg cgaccatgat tcatttttcg gcgctttatc gttccacgtg
     5761 cggcttgatt ttacgggggt ccccgccgaa acctgccccc ggccgcggtc aaagtcaaag
     5821 gcggcaacgg catctctttt aattttaaat ataagcgatg ccggctgcat cattgcgacc
     5881 gttttcggcg cgacgccttc ctcggtcctt ttcggcgcat tgagcacccg ccgcacccgt
     5941 gcttcggaaa gccgataaaa attcatcttc gcccgcgcgt gatgcgtcca cgaaacggtt
     6001 tttgattttt tgattgcaac cataaaaaaa tattgatacc gcttacgttg
//
LOCUS       DAIDDT010000043         6029 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_43_length_6029_cov_5.087534, whole genome shotgun sequence.
ACCESSION   DAIDDT010000043 DAIDDT010000000
VERSION     DAIDDT010000043.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43_length_6029_cov_5.087534"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1744 a         1434 c         1594 g         1257 t
ORIGIN      
        1 gcatgcctga tcaagaaaaa atcaaagaat ataaaaccaa actcgagcag gaacggttgc
       61 tcttggttga tgaaataaaa cggaacgaaa caccgacgga ttttggagac gatactgatg
      121 acgaatccga tgaaaccgaa aatttcggga accagcttgc catggcgcaa ggcctgaaag
      181 atcggttgaa tgaaatagat attgctcttg gaaaaattca atcgggggga tatggcatct
      241 gtgaaaaatg cggcaagaaa atagaaaaag aaatacttga catagaccaa gaatctcgtt
      301 attgtaagga atgtaaattg aaggaagaat aataaggcta tcggcttgta tttaaaaaat
      361 ttctggttat ttttttggat tatataatcg cattgcctcg ataaaaatat tcccatcaga
      421 acgattgttc tgatgggaat atttttatcg gcaaagaaag tctttaggat cttttaatcg
      481 ccggtatgat aatcgtaagg gatgatacaa aaccttgcga gggtctattc ggcggagctg
      541 gaaggtattg atgcgcggct tatcgaagtg gaagccgacc tgaatgtcgg actccatgct
      601 ttcaatattg tcggccttgc ggataaagcg ttgaatgaag cgaaagagcg ggtgaactcc
      661 gcgctgaaaa attccggcgt taaaccgccg aaccgcgaga accggagaat caccataaat
      721 cttgctccgg ccgacgtgaa aaaagcgggg tcgcaatacg atcttgcaat cgccctcggt
      781 tatctttgcg caacgaaaca aatcggcgag tttgaagcgc gcgacaagat atttcttgga
      841 gagcttgcgc tcgacggacg tttgcgcccg atcaatggcg cgttgagcat cgcgcagatg
      901 gcggcatcgt tgaatttcgc ttatgtgttt ctaccggcag aaaacgcgaa tgaagcggcg
      961 gcgataaaaa acataaaagt agtccctttg gaaaaactcg atgacgcgat tttctttttg
     1021 gagggaaagc ggaatattct tccgaaaact ttcaaagagg aggaagcgga aagaactatg
     1081 gcgcctgatt tttcggaaat aaaagggcag gaaaatgcga aacgcgccct tatgatcgcg
     1141 gcggcgggcg gccataatat cctgatgatc ggaccgccgg gggtcggaaa aagttttctt
     1201 gcgcaagcgc tcgcgggaat attgccggat ctcgcgacaa gcgaggcaat cgagatcacg
     1261 aaaatcttga gtgcgggcgg cctcggcccg cgcggcctca tccgcagacg cccgttccgc
     1321 gcgccgcacc aaacggcatc caccgcggcg cttatcggcg gcggcgcaga cccgaaaccg
     1381 ggtgaaatta cgttggcgca tcgcgggata ctttttttgg acgagctgcc ggaattccaa
     1441 aaaaatgcat tggaagcgct gcgccagccg atggagtcgg ggatggtgca tgtcgcgcga
     1501 gcgaagcacc gtttgatttt tccggcgaaa ttcacgctgg tcgcggcgat gaatccgtgc
     1561 ccatgcggct attatggcgc cgctgaccgc gcatgcacgt gttccgcgta cgaagtgata
     1621 aaatatcaaa agaaaatctc gggaccgcta ctcgaccgca tcgatctcca gataaaagcg
     1681 ggaaacgtga agatcgacga cctgcggaag ggagaacggc aacaaccgat gaacgattcc
     1741 ataaagacga aagtggaagc ggcacggaac gcgcaacgga aaagatttgc ggcaagaaat
     1801 ataaaaatag agacaaacgc ggagatgagc tcgcgtgaaa cggaggatat gatgaacctt
     1861 gatcctgatg ccgaaaaatt tttggggacc ttcaataaaa gcgcgatctc gccgcggagc
     1921 tatttccgca tcttaaaagt ggcgcgcacc attgcggaca tcgacggaaa agaaatggtt
     1981 accgcggaat atctcgccga agcgttcagt tacaggatac gggatgaaat atagtggacg
     2041 tctggtgcga aaaaaaacga gccctcgttg tgagggctcg ttttggagga ctattttcgg
     2101 tgcgattttt cagggagact aaaaaaataa ttactgacca agtttgaact ttatttgaac
     2161 gccaaagaaa atgccgtgtc cgatgggccg ctgccgctct tcgtcgcata tgccaccgtt
     2221 cccgattgtt ttgacggagc gcttgccagc atgcccgggt cttttgcttc gcccaaagaa
     2281 agaaactccg gcgcggacaa caggaaggaa ttggccgttt cctgcgacga cgtccccgac
     2341 aaaatcgggc ataagggagc gcttaccgac gcaccgtcgc ccgctccata agccacgttt
     2401 atcgcgcttt ccgtaacgga agaaactccc ggcgcgccgc tctcctttat aacgcccgta
     2461 acaccggaaa acgcaaagcc aaaaagcgaa gccgctacaa aaaaaagaat cgccatcaac
     2521 ggcttataag cgtccttctt gagttgtcca acaaaaatac taatagtggt attatagcat
     2581 gatggcaaat aaaactcaaa gggggggtgt ggaaaagtcg ccaacgctca atcccgccca
     2641 aaaagcagcc gcggaagcgc cattctcgcc gctccttatc gttgcaggcg ccggaaccgg
     2701 aaaaacaagg acgctaataa gccggatcgc ctatttaata gagcgtggca ttccgccgca
     2761 taagatctgc gcgatcactt tcaccaataa agccgcgaag gagatggctg accggaccca
     2821 tctcatgatt ggttcaaaaa aagggccttt tatagggacg tttcactctc tgggcgcccg
     2881 catcctgcgg gaagagtgcc gcgtactgga aagaaagccg aacttcacga tctttgacga
     2941 ccatgattcg ttcaatctcg tgaaaaaagc ggtcaaagcc gtcctgccga aaagcgaagc
     3001 aataggcgcc gaaaagaaaa aagaagcgcc ggcgatgttc gcgcaggaaa tatcgaaaat
     3061 aaaaaatatg gcgggcattg atccggtcgg tcgccctcag aaaaacaaga atccgcgttt
     3121 tgaaaaaaat caaacgaaca ggatggaaaa tatgaattcc caccggagct tgtcctcgtc
     3181 aacaatcgaa ggcgaggacg acggagggga tgggaagcat catggatcgt ccgccgccac
     3241 ggacgatgac aaagacgaga aaagtgcggc gacgcattca atcttccgaa aatacgaagc
     3301 cgcgctcatt gaaaataacg cattcgactt tgacgacctt atcgaaaaaa cggtggcggc
     3361 cctgaaaacc tatcccgaca tccttgaaaa atatcaaagc cgctacgatg ccattctggt
     3421 cgacgaatat caggacatca atccgcggca atacgaactc gtgcggcttc tcgcccaaaa
     3481 gcacggcaat gtgagcgtgg taggggacga cgagcagacc atttacagct ggcggtatgc
     3541 cgacatcggc ctttttctta atttcgaaaa acactggccg ggcgcgaatg ttttttttct
     3601 tgaggaaaat taccgctcga cgggaaatat catcaacgcc gccggcgcgg tcgtaaaaaa
     3661 taaccgcttc cgggcgccga aaacattatg gacgaagaac acgccgggcg cggcgataaa
     3721 acttttcgaa gcgtggggag aaaacgaaga agcggaatgg attgcgcggg aaattcaaaa
     3781 tgaaaaacgg cattcttatg tcatccccgc ccttgatcat ggtcgagggc aggcagaggc
     3841 ggagatccac gttttaaaag tgaaaaatga gtcggtcacc gttctttacc ggaccaatgc
     3901 gcagtcgcgc gcgatcgagc aggcgctcat ccggcataat atcccgtacc gtattttcgg
     3961 cggattgaaa ttctatgaac ggcgcgaagt gaaagatatc gttgccgcct tgcgctacgc
     4021 atccaatccg cacgacgcgc caagcagaga gcggctcgaa aaaaatcttt caaagaaaaa
     4081 atttgccgag ttcaaaagcg cgcttgcgaa agcaggcaat atgaaaccgc aggaaatcat
     4141 cgcgcttttc atggaaacat tccattattt cgattatctc aaggagtatt ttatgaatgc
     4201 cgacgagcgc gagggaaaca tcgccgagct tatcgcgttc gcgtcggaat tcgaaaacct
     4261 gagcgagctt ctggaaaaaa taatgctgct ccaggtggcc gacgacgaaa acaagaacaa
     4321 cggcaaagaa cggggatcgt cgccggaaac ggtgaatctt atgacgatcc atcttgcgaa
     4381 agggcttgaa ttcgatacgg tgttcattgc cggagcatcg gaaggcctcc tgccgcacgg
     4441 ccgctcgctg cgcgaagaac gctcgctcga agaagaacgg cggctgatgt atgtcgcgat
     4501 gacgcgcgcg cggaaaaatc tcgcaatcag tttttacggc gacccgtcac ggtttatcgg
     4561 tgaaattccc ggagagtatg tcgcgcttca gaacagcgcc gccggcgcaa ccgatttcac
     4621 ggacaccaac tcggccgcgg acgtcggtta tggccgtaat tttgaagaag aggattatat
     4681 agaattttga accatgcaaa aactcggaca gcattttctg aaaaataaaa aaatagccgc
     4741 ggaaataatc gacgcgctcg cgatgcgcgc gaacgacgtt gttttcgaga tcgggccggg
     4801 acacggagag ctgacggaaa aaatgaaaat ggaaagtgaa aagcctgaac ttcgggaaaa
     4861 taaaattaaa atcatcgcga tcgaaaaaga cgaaaaactt tatgaaacgt taaaaaaacg
     4921 gtatgaaaac gacgcgctta tcgcaatcat tcacggtgac gctctcgcga tactcccgtc
     4981 gctcatcgca aaaaacagag gtcctgaatc ggactatctt ctcgtcggca atattcccta
     5041 ttacatcacc ggccatcttt tgcgcattgt cagcgaactg gaaaacaagc ccgagcggtg
     5101 cgtattcatg gtccagaaag aagtggcgga acggattgcg gcgcgggaga aaataaaaag
     5161 agggacggcg gacgaaaacg agaaaggtgc gggcatgaac cggcttgccg caagcgtcca
     5221 gttctgggcg gaaccgaaga tcatcgcggt agtgccgaaa aaagatttcc ggccggcgcc
     5281 ggacgtcgat tcggccgtca ttcttcttgc gcggaaaaac agcccgctgc cgtgcgatgc
     5341 cgatcggtac tatgccgccg tgcgcgctat tttttcccag ccgcgcaaaa ccgtactcaa
     5401 taatatctcc gaagccatcg cccgcgcgcc gctgaatatt cgatccgcgc gccgcgccga
     5461 accgcaaaaa aaatcttttt tttcagacga aggaatggcg acgaaaaaaa aagaaacggt
     5521 tgcggcggcg cttgaagcga tcggcatcga tccgttatcc cgcccgcaaa atctttccat
     5581 tgaagatatt gcgacaatcg cgaaaaaaat tttttaaaag gcatttttat tgcgttgccg
     5641 tccgttatta tagagataaa aaactgttgc gcggtcccgt gtttattcca tcttaaggct
     5701 catccccgtc ccaaggggcg gggatgagtc aatcctctcc gccttcgcgg ggacaggatc
     5761 cgctctttat tgttaattat tatccctgcg gcacggaggg cggagaagct tctaacgcat
     5821 tgatgcggcc atgtggccgc ggggagcttt atttttgcgc tcttgcagaa gtatagatcc
     5881 tcgacccagt cgaggacaac ggaaaagaat gaatggtcga cggtttacgg tttctaatgc
     5941 cctaaaattt ttatttgaca aatatatatc ctgtgctact atagaaacag tttcttgaaa
     6001 acttaattgc gtttgccgta acagagagg
//
LOCUS       DAIDDT010000044         6007 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_44_length_6007_cov_6.298820, whole genome shotgun sequence.
ACCESSION   DAIDDT010000044 DAIDDT010000000
VERSION     DAIDDT010000044.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44_length_6007_cov_6.298820"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1559 a         1429 c         1688 g         1331 t
ORIGIN      
        1 caggtgaaag tgacgcttat cgcgaccggt tttagtggcg caggcagcca ggtgaactcg
       61 ctgttcggcg gcggacatta cggcggcgag cggcagacga tattcggcag gacatccgcg
      121 tcttcaggcg gtgcatcgca ggagagcgaa aatatttttg aaaaaaaaac tttcccgcgc
      181 gatgacgcgc gcgctgcgcg aaaggaaact gaccgtttcc gcgcggcgga agaaaccgcg
      241 gcgggcgctc cgtcaaaaac agcctcgttt tcggcgaagg acgaaaagtt cctaaaaaaa
      301 gacgaaaaaa aagataagga aaaagaatcc gaaccggaag aagccgaccc gtgggacatt
      361 ccggcctttt taagaaggag aaagaaataa aaaacatccc cgctaccggg gatgtttttt
      421 atcattttat gatgcgccca tgtttcattc cggcgttttt attgtttcga aaaacgcgcc
      481 atgcgaacgt ttgccgggaa ttcgcgtttt cccgatccgg acttctgata tttcccgcgg
      541 attcaaaatt ccggaacagc ggacgagcgt gtcgggtcaa tttatataaa aaatctcgtt
      601 ggtcggcgtt ccggcgggga tggcgcttga cgcttcggct ggcaggaggt aatggatgtt
      661 ttttgccgca gcgtacgtta cgccgtcagg aaggaaagaa tcggtgataa acatcgtggt
      721 ttcgacgggc atggggagtc cgcgcgtcat aacgccgccg gcattcacga caacatattt
      781 cggcatgctt gcgggaagcg cgttgatttc gtttccaatc gcgacataat cggcgttgaa
      841 agcgccggag acgttcgggt tgtgcgccca cgtgacgaaa taagcaacgt agccgccgaa
      901 gacggcgacg tacgcgagga atatccacga aataattttt gccaggtttt catggcccgg
      961 cagttttgcg acaagccatt ggtaaagcca tatgccgccg atcgccgcga agatcatcgc
     1021 aggcggacac ataagaatgg atcgcagggc atgaggaatg ccttcgtcgg aggccgcggc
     1081 cggcaaacca gcgagaacga tccatgaaaa aacaaggagg agcggaaacg ccggaagaat
     1141 gttttttcca tcatcgcgca ttgttttttt acgccggctt ttccagagag aaaagagtcc
     1201 gatcgcgatg ccgaggatga aaagaattcc cacgggccag aaaagttccg gcgcgccgct
     1261 gatattctgc cgccagttat tatcgccgtg gaaattaaac atcaatgcgg tctttacaac
     1321 gtttacggcg aaatcgcgga cgggatttgc cgcgttcgtg accgatattt ccgcggtgcg
     1381 tccgaagaag tcggaggggt gtttcaaaaa ataccatccg atcggcgccg ctgtgatgaa
     1441 aacgagcgcg ataaaaacag cgacgcgttt ccagaaatct tttcctttcc ggaaaaacgg
     1501 gatgaataag agaaaaagaa gcggcgtgat gcggtaggcg atataagtag agaagccgag
     1561 gccgtagaca atgccggcga taatagcgca ggcggttgcc gcgcgcgaag aaacggcgcg
     1621 tagcgctctc ataaaaaaat agagcgacca gacgaggaac agcggcgcaa ggatggcgcg
     1681 gaagccgatg cgggaaaaat tgatatgcca gaacgaggtt gcgaggaaga atgcggcaag
     1741 cagagcgatg gcggaaccgg aaaccgaaaa ccggaaattt aaaaacgatg gcttaacggc
     1801 ggatgctttt tggtccgggc cgtggtccga ttttttgcga tccgcttgct ctgtctcgcc
     1861 ttctctgaac agttcggcga cgaggaagta aagtccgagc accgtcaaca cgcccgcgat
     1921 cgcggcggga agccggatga tccacggttc ataaacgggg aattctttga gtatgacggc
     1981 gatgatgttc acatagagcc cttcgcggcc gttgtcctcg gtgtaaaata ctttgaactg
     2041 tcccgtgcgc gcgacttccg cggcgttatt gccgtccata gcttcgtcgg gatagagccc
     2101 cgggggcacg gttgtgaagt ggtaaaaacg gaagaagatt gctataatca ggataccgaa
     2161 caggaaaacg tatttttttg tattggacat acggtcatta taacaaatcg acgcaattct
     2221 tattgcgtcg tccccgcgga ggcggggatc catgctttct tttagacttt cggctaccag
     2281 ccatcagctc gatagaaagc gttgatcctc aaccgagttg aggacgacag aggagagcgt
     2341 tctcacccat aattcataat tattctcatc aatgccggaa gaaacttctc catttgaaca
     2401 tgcggcgctc gaggcggata tgaaacgcct tacggaagag atcggcaatc atcgcggttg
     2461 ggcggagatg aaagatgcgc ccgaaaaaca attactcaag gaatcaatca gggcgtttcc
     2521 gcggtttgaa aaaaacggtc cggcggccgc tgtgccggcg ccggttccac cgccaaaagc
     2581 gcagggcgct acgcagagcc ccttgccgga ctacgcgcaa agcgcggcgc cggaagtgaa
     2641 gcttgagata gaatatcttc tcgatcttgc gctgcaccag ggattcggga aggcgcttgc
     2701 cgaagcggac aagtcaccgt acttcgttca ggatgcattc cacgacgccc tggcggggaa
     2761 actgtatccg gaactgcaaa agagaggaat tataaaatag agagcgtttc ctctgtcgtt
     2821 cccgcggagg cggggatcca cgctttaaaa actcagcgat ttgagattta tgattcacga
     2881 aaaaagacgg gaagcttgga tattcgcctt tgcggggacg acagatggga agcaaaaatt
     2941 taacggttag gcattattat atgtatatta ttttctttat catcgcttgc gccattgccg
     3001 ttgtcgcgat tttttttatc gtccgcaatg ccgcgcgaaa acgcatcgac gattcgcttc
     3061 gttcggcgct ttttctcatc aaggttccga agccggacgg caacgataag ccgggaaacg
     3121 ccgaacacgg ggatttcaag acagaacttg cgcattttga acagctgctt gcgggacttt
     3181 ccgcgataaa aaaaccgttt gcgttcgaga ttgcggtgcc gcatgtgggc gaagagatac
     3241 atttttatct ttccgttccg aagctggcgg gtgagattgc ggcgaaacag atccagggat
     3301 tatggagcgg cgcaagcgtt gccttggtgc aggatgattt tactattttc gacgcgcacg
     3361 gcgcggcggc cgcggcgtat atcgcgcaaa aggaaaattt tgctttgccc gtaaaaacat
     3421 atgccgagct tggtgtcgat tctttcgagg cggtgctcgg cgcattcgcg aagatcaacg
     3481 aagccggtga aggcgcggca ttgcagcttg tcgtgcgtcc ggcggcgaga gaagcgaaaa
     3541 agaaagtcca gggatatatc accgctttga aaaaaggaga gccgctgaag aaagtgttcg
     3601 gccatgaatt tccgctttcg ttttccgagg tggcgcatgt tttgaatccg tcgaaagagg
     3661 aagaggaaaa aaaggaaaag atcatcgacg aagaggcggc gaaagcgctg caaatgaaga
     3721 tcgcgaagcc gcttttcgag gtgaatgcgc ggctcatcgc gtccgccggt tcgcaattcc
     3781 aggccgacga tattctggac ggtcttgccg ccggtttcag ccagtttgcc gcgccggagc
     3841 ggaacgagtt tcggatcgta aagcagcgta atccgaaaaa actcattgat gatttcattt
     3901 tccggtcgtt cacgcccgcg cagacgatga cgctttccgc ggaggagatc gcgagttttt
     3961 atcatctgcc gattgcgtcg accgagacgc cgcgcgtgaa atggctgaag tcaaaagaag
     4021 cgccgccgcc ttcgaacctt ccggcgaaag gatttttgat cggcgagagc gtgttccgcg
     4081 ggcagacgaa gccggtctac atgacggatg acgaccgccg ccgccatatt tatatcctcg
     4141 ggcagaccgg taccggcaaa acgacgctga tcggcaacat gctcatcgag gatatcaaag
     4201 cgggaaaagg gctcgcgatc atcgatccgc acggcgacct tgcggaagac gcgctcggat
     4261 tcgtgccgaa ggagcggatg gatgacgtca tttacttcaa tccggccgac atggagcggc
     4321 cgatggggtt gaatatgctc gaatataatt tcaaccgtcc cgaggagaag acgttcatca
     4381 tcaacgagat gttgaatatt ttcgacaagc tgtacgacct gaaaacgacc ggcgggccga
     4441 tgttcgagtt gtatctgcgg aatgcgctgc tgcttttaat ggaagacatg ccgaacgagc
     4501 cggcgaccct gacggaagtg ccgcgcattt tcaccgacgc ggcataccgc acccggaaac
     4561 tacagcgctc gaaaaatccg ctggttgtcg atttttggga aaaggaggcg tcgaaaacga
     4621 gcggcgacac gtcgctcggt aacatgacgg tttatgtttc ctcgaagttc acgccgtttc
     4681 tcggcaacga ttacatccgt ccgatcatcg gccagccgaa atcggcgttc aatttccgcg
     4741 aagcgatgga cagcgggaaa atattgatca tcaacctttc gaaagggcgc atcggcgaca
     4801 tcaacgcgtc gctgcttggc atgatcttta ctgggaaaat cctcatggcg gcgttgtcgc
     4861 gcgccgatac ggcggacagg gaaacccgcc gcgatttcaa cctttatata gacgagttcc
     4921 agaatttcgc gaccgattcc attgccacca tcctttccga agcgcgtaaa tatcggctga
     4981 accttattat cgggcaccag ttcatcgcgc agctcattga taagataagg gatgccgttt
     5041 tcggcaacgt cggttcgcag atcgttttcc gcgtgggcgc tgacgacgcg gaatttatcg
     5101 ttaaacagtt cgcgccggtg ttcagccaga acgatctcat gaacatcgac aacatgaacg
     5161 cgcatgtgaa gctgctcatc aacggccaga cctcgaatcc cttcactatg cgcatcggca
     5221 cggcatggtg gggcggcggc gaccgcgcgc tcgcggaaaa gctcaaggaa tattcgcgca
     5281 tgaaatacgg cgtcgaccgg caaacggtgg aggatgaggt ctatggaagg ttgaggggat
     5341 agattctttt ctgtcgttct cgcgaaggcg aggatccacg cttaggattg attgatggta
     5401 gaaatctgta tgcgggcttt taaatctctg cttgtcgcac caacggtcac gttaagcggt
     5461 cattggtgtt tttaaagaaa accgtcagat taaaaaatca taaattcaaa tttgacgcga
     5521 ttttaaaatc cgcctattat agcaacatgc gcattatgca cttacaatct gcggtttgga
     5581 aagaggggaa attctacgtt gcccagtgtt tgaacgtgga tgtttccagt ttcggcaaaa
     5641 ccaaaaaaga ggccttgaaa aatttacagg aggcgttgga gctttatttt gaagacaaaa
     5701 aacctggcaa aatcacgcgc gtggaaaagc cggaattgac cgaattatcg gtggcacatg
     5761 cctaaacttt attcttccac taaaataatc aaaaccctgg gaaaccgggg ttttgttttt
     5821 atatcgcaaa aaggcagcca tgctaaatat cgcaaatatg gcagttctgt gttaacagta
     5881 attattcctg ccgacagaaa acaggttcct atcggaactt ttaagtcaat attacggcaa
     5941 tcaaatttac aaatagaaga ttttgagaaa tagatttttt ccgctatttc tgaatatggt
     6001 atcattg
//
LOCUS       DAIDDT010000045         5943 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_45_length_5943_cov_6.822537, whole genome shotgun sequence.
ACCESSION   DAIDDT010000045 DAIDDT010000000
VERSION     DAIDDT010000045.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45_length_5943_cov_6.822537"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1466 a         1600 c         1436 g         1441 t
ORIGIN      
        1 atatatcctt ttgctgctcc ttttataatt ataaaaggaa atatcggaaa acacaaaaaa
       61 caagaatgca tgacagcaat gctttctttc gatgccttcc ttactgaagc aggcggctcc
      121 ggagtattat ccattttttc gaaagacgga taaaaaaacc aaaaccccct tgcgggggcc
      181 gtggccaaac agaatcgtgt gggacatgcc tcaatcgacg tcgtcgctaa ggtatgctcg
      241 cactcattcg ccggaatgat agcatgaaac ctttaaaaaa gcaaatggcg cgggtaaatg
      301 cgttatttaa gttgccgcag catttccgta accgaccggg cattcacaat gtcctcgcgc
      361 gtcgcccagc cgcggcgggc ctgcgcgatg ccgtattcga gataagcaat atgcgcggac
      421 gcgtgcgcat cggaatcaat cgccatttta acgcccgcct cgacgcattt ccggatatgg
      481 tcatccttca aatcgagccg gtcgggaaac gcatcgattt ccatgatcgt ccccgtgcgc
      541 ttcgccgcgg ttataagcgc atcaatatca atgtcgtatg ccggacggcg gttgataatc
      601 cgtcccgtcg gatgaaaaag gatatccgcg ttcgggttcg ccatcgcgcg ttttatccgt
      661 tcggtctggt ctttgcgcgg catattaaaa aaagaatgca ccgcaacgcc cacgacatca
      721 agcttcgcga gcgcggcatc cgaaaggtcg agtgatccgt cttttaaaat atcgcactcg
      781 ctccccttca aaatttttat ctttccggcg aattttttat tcagccggtc gatttccgcc
      841 atctgtttca ggagccgcgt ttcgtcgagg ccgtgcgtca tcgcgagccg ttttgtgtgg
      901 tcggtgatcg cgatatagcg caggccatgc gccatggctt ttttcgccat cgtctctatg
      961 gaatcggagc catccgtcca atcagtctgt atttgaagat cgcccacaag atcgccgtag
     1021 ccgatcagtt tcggcaatgt atggttccgc gccgcttcaa gttcgcccgt gttttcgcgc
     1081 agctccggct cgatataatc catgccgaat tttttataca gctcttcttc ggtcttgccg
     1141 gcgatccatt ttcccgtccg cgtttgtccg cgttgcaatc cgcggtggcc ggcaaacagg
     1201 ccgtattcgt tcagtttcca tcctttttgc gccgcaatta cgcgcaaagc gacattgtgc
     1261 tctttcgatc cggtgaaata attgagcgcc gcgccgtacg attcctcgga aaccacgcgc
     1321 agatcgacat tgatgccgga ggaaagtttt accgacgact tcgttgcgcc atgggcaatg
     1381 actgctgcga catcgggcat tgcgacgaaa aaatccataa tcggtttcgc attttttgaa
     1441 ataacgagca tatccgcatc gcccaccgtt tctttcctcc gtcgcaccga acccgcgacg
     1501 acaatctttt ccacgccgcg cagtttccgc agacgctctt ccacgccgcg gatttccggc
     1561 atcacgaaac cgagcgtgaa ccgcccgccg gaacttttcg cgaacgaaat tcctttcaaa
     1621 attttttctt cgcttttttc accgaaccct tccagcgcgg caatctttcc agcgtccgct
     1681 gctttttcca ggtcggcgag acttttaacg ccaagttttt cataaaggcg cttgatagac
     1741 ttcggaccaa gcccttccac tttcgcaagt tcggaaagat caacgggcgc tttttttttc
     1801 aatccttcgt aatacgcgat gcggccggtc ttcaagaatt cctctatctt ttccgcaatg
     1861 gaaacgccga caccctgaat ttcctcgagc gccttgatgc cgcctgtttt ataaattacg
     1921 gcaagttcct cttcaagccc gtcgatcgcg tccgccgctt tttcgtacgc ccgcggcttg
     1981 aaaggaacgt tctgcatcgc gagatattcc ccgatctcgc gaagcagtcc ggcgcacaca
     2041 tggtttgaaa tgttttgcat tgatactata tttcgctcgc gctcggcatg tgatctttta
     2101 taaccacttc ggtatgtgtt ggtttcggaa aaacaagatc gggtgctgtg ataagaataa
     2161 gcgccgcaat catggataat attcctgccg cgacaaacac cgcggtatac ccgagctctt
     2221 gcgcgataag accactgaaa agccccgcta ttcctgccgc gacaccgcct acggtgctgt
     2281 caagcgacca gtcgaaagag attctttcct tatcaagatg ccgcgaaaat attgccgacc
     2341 atgcgggaac ataaaacgca aatgcgattg cgtggactac ctgcactgca taaagctgcc
     2401 agacttcgtg gatccagctg aaagaaaacg cggcaagtgc cgcaagaaaa agtccgccga
     2461 caagcgcggt gaaatcgtcc ttttcgcctg gcgtcgtgtc gagaaaatta gcgataggga
     2521 tttcaagcac ggatttcagg atccaataaa ttgccgccgc aataccaacg gtcagcaaag
     2581 tcgcgccgac aactttttga atgataaaaa cagagaaaac cggttcgata aatccccatc
     2641 cagcaagaaa aaaaagatct gaaatcacga aatacttcac gatgcgccct actttgatat
     2701 tgatatcaat gcgtatcttc atgagataag tataacatag gacttacgca cttgtaccga
     2761 atacaaagca ttttctctct gtcatcgtcc ggcttggccg gacgccccat acttctcata
     2821 ttgataaata agccgaaccg aaagattgaa aacggaaaac ctgtctcaaa aataggacag
     2881 cgaggatgaa cgaaaatttc cgtaagatca gcggactttg cttgaaaact gcttttgagt
     2941 gaaacgggtt ctaaaacaaa tgcatcacgt cgcggaccgt aaggagcgcc ataagcgcaa
     3001 gcaggaacgc aaagccgatg ccgttcatcc agccctctat tttttcgggc accggcgacc
     3061 cctttatttt ttctatgata gtcatcacga accgcccgcc gtccagcgcc ggaaacggga
     3121 tcaggtttac caccgcgaga ttgatagaaa taatgccgag aaattgtatg aaatacgtcg
     3181 cgccgatctt ccccgtttct tcggcgacgc cgaaaatgcc gaccggcccc acgacgcccg
     3241 ccatgagcga cccttgcgtg acgagctgtt tcacaagctg gccaaacgcg gtgacggtaa
     3301 gccacgcaag gtcggcggtt accttcaacc cgtcccaaag cgccgcaaaa acattctcgc
     3361 cgggccggcc ggcatcctca agatacacgc caagcgcgcc ttcatttgcc gtcgtcgtca
     3421 cgcgcggtac gacgctgatg ttcaattccc tgctgccgcg caggacgtcg acattaatcg
     3481 tctgtccgcg atgcgcgttc acgaaattta taaagtcctg cgaatcggga gcgtttatcg
     3541 tcatagcgcc ctgcgcgaga ccggctcctt ttacgacgtc gccctgcgcg agaccggcca
     3601 ttgcggcagg cgatcccggt tcgatgtcgc ttatgaccaa cgccttcggc gcggacaggg
     3661 taagcgcgaa agagatcaaa agccagccga tgacgaagtt cataaaaacg ccggcgagca
     3721 gaataatgct ttttttccac gccggctggg cataaaaaag acgcttttta tcagggacat
     3781 tttgttccga attccgattg tccgattccg cattctgttt tttattcctc tctccgtcct
     3841 gcgccgcttc ttccgtcatc gcgaactcgc cgcgctcgcc cgaaattttg acgaacccgc
     3901 cgaacggcag ccagttcacg ctgtactcgg tttcactccg cgtctcgcgg gaggttcctt
     3961 tattttttat gcgcctccat gcgaatgcgc gcggggggaa tccgatgccg aactcgtcca
     4021 ctttcaagcc gaataatttt gcgcacgtaa aatgccccgc ttcatgcgcg agaatcagcg
     4081 ctgaaagccc gataataacg aaaagaataa tgagaatagt catagtgcgg attatatccc
     4141 atccgcgccg caagcgcaaa aaaacggctt gcggatattt ctaacgcccg ttttatgaga
     4201 acgccgccaa tgatttcatt cctcttccga aaattcttcc tcttccgcta ttttttggcg
     4261 tccgcgccct tatccttcca gataagtttg tccagcccta ttttttcggg aattttgttt
     4321 acgtacggaa tatatgggaa tgcgatgagt agcagcacaa ggaaacccaa tatttccgcg
     4381 ccgtcgcggt cctgatcagg atcgttcgcc agaacggtat tgtcgaggaa gccgagcggg
     4441 gcgagccacc aggcaccggg cggcagaagc cctccatttt cttcacgcag catgccgtat
     4501 tgtccggttg tcatattgag atcttccgct ttcgcatcca tgatcccggt gtcagacaga
     4561 aacctcgtca cataggtccg gttatcgctg cccgcgcttt cgccttgcac tatcgattca
     4621 taagttccgg tttgggcaag tttcgtcaac gcgcttaccg tcgcgatgac cggattcgcg
     4681 ttcgccatat cgagcgcgcc gttgttattg aaataatcct ccgcggcagc ggtatcggct
     4741 ttttgcgttg cgggatcccc gctttcgaac gcggccatgg aatccgcgcc gcccgtgatt
     4801 ttcagatatt ggctgaacgg cacggcgaca tacacctgtt cggtgtcgta cgtataggga
     4861 tcgatggtgt ccatgtaggt tgccgtatcc gaagtatggt ctatctcggc aaccagtgtt
     4921 tttgcgacga gcgcaggatc gtcgtgcgca acactttgta tggttgtcgg aataataaac
     4981 ggcgatttga acaccgcgcc gagaacaaga atggcgagca caagaaccgc gccgcgcaaa
     5041 aagagcgcgc gacgattggt tttcacgatc cggtaaggaa cttccttttt gaacggcacc
     5101 gcaatgccgc gcgctttaac aagaagatag tgcaaaaaga gaagcgccac tatcgcaagc
     5161 ggaatggcga cgatatggat gccgtaaatc tgggaataat tcaggttgtt cacgatcttt
     5221 cccaagccgc ttccgttata aagatcggat gcctggagca cccgccactg ggaagaaaaa
     5281 tcgccgcgta tgccatagcc gaattccgct tcgataagcg ccgcgaaaag cataagtgcg
     5341 ccgagaaccc atgtgaagcg tcgtggcgga cggtaacccg acgtgaaaaa gacaatgaga
     5401 agatgcaaga gaatgaaaaa gatgaacgct tgcgccgccc acagatggat gctgcggaaa
     5461 tacagtcccg ttgccgtcgt gagccaccaa ttcggtcctt tgaaagccag caccgttccg
     5521 gaaataatga ggacgagaag gctcgtcatg gaaaggaacc cgagggaata atagatcttg
     5581 ttggcgtacg ccggaatatc ctgcagcatc agatcggaca ggatattctt gaacgatttt
     5641 ggccggtcgg tttgatggtt catagatggt cgaatataag tatataaacg attacgaaat
     5701 gcggcaaatt accatttttg atgaccattt tcgataactg ctcgcctgca taaataaacg
     5761 gctgaccgtg tggtacgaag ttggaacgat tttaagtact tatgcttaag ctttatatta
     5821 catctaattt atgataaaat ggaggaaata ataaaaaata ggaaaacatg gaagaacagt
     5881 ggtatacaat aactggcggg atagatgtta acacagcgca taatttaatt acctatatta
     5941 acg
//
LOCUS       DAIDDT010000046         5937 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_46_length_5937_cov_6.649147, whole genome shotgun sequence.
ACCESSION   DAIDDT010000046 DAIDDT010000000
VERSION     DAIDDT010000046.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46_length_5937_cov_6.649147"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1496 a         1390 c         1587 g         1464 t
ORIGIN      
        1 attttaaaat aagaaaataa gcacaatgaa aaaaacacgc attgcgataa acgggttcgg
       61 acggatcgga agattatttt taaaatcggc atttgctacg gataacctcg agatcgtcgc
      121 cattaacgat ctcggcaatc tcgaaaatct cgcctatctt ttgaaatacg atacggctta
      181 cggccggtac gataaagcgg tggaagccga tcttgcgaaa gggaaccttg tcatttccgg
      241 cgccgaaata aaattcctgc agatcaaaga cccggcgcag cttccgtgga aagatcttgc
      301 tatcgatatt gtcgtcgaat caaccggact ttttgaatcg tacgagaagg cgatggtgca
      361 tgtgcaggcg ggcgcgaaac gcgtggtgat tacggcgccg gcgaaagacg ccgacggtcc
      421 tgccggcaaa acggtcctca tgggtgtgaa cgaggacgcg ctcaaaacgt gcgcgataac
      481 ttcgaacggc tcgtgcacca cgaattccgc atcgcctgtt atacagatcc tttcggaaaa
      541 tccgggtatc ctgaaagcgg tgctttccac ggtgcacgct tacacggcaa cgcaaagtct
      601 ggtggacgga cccgttcgcg gcggacacga tttccgcaag ggccgcgcgg ccgctcacaa
      661 cacggttccc gaaacgaccg gcgccgcgat ttcggtcacg cgcgcgatcc ccgggctcgc
      721 gggccgtttt gacggtgttt ctttccgcgt tcccactatc accggttcca tttcggatat
      781 cacgtttctt gcgaagcgaa aaacgagcgt tgaagagatc aaccagattc tcaccgaagc
      841 cgccgcatcg ccgcgctggc agggaatttt taaagtgacg accgacccgt tggtgtcatc
      901 ggatatcatc ggcgaaccgt acggcgccat cgtcagtctg gatctcacaa aggtcataga
      961 cggcgatctc gtgaacgtgt tttcctggta cgacaacgaa gcggggtatg tttcgacgct
     1021 cgtgaagcat gtgcagaaag tcgcggaaat cctgtaatca aaattttatc gtatgaccat
     1081 ttacataggc gcggatcatc gcggatttaa attgaaagag cacctgaaga gtgttttaaa
     1141 gaacgaggcg tatgatgtcg tcgacgtcgg tgccgtggtg tacgataaaa acgacgacta
     1201 tcccgattat gcgcggccgg tcgcgaagca tgtaggcgcc gcgccggaca ccgatcgcgg
     1261 gatcctgatc tgcggatcgg gtttcggaat gactattgcg gcgaataaat ataaaaatgt
     1321 ccgtgcggcg cttccggcat cggccgacca tgtgtatcag gcgcgccatg acagcgacgc
     1381 gaacgtgctc tgtctcgcgg ctgattttac ggatgaagaa acggcggacc agattgtaaa
     1441 aacatttctc gcggcaccgt tcgggaaaga ggagcgctat gcgcgccgtc tcgagaaaat
     1501 aagggaaagc gaaccagaga gataaacgta aagcgttgca gtgttgtaat gttttagtga
     1561 tggagcggcg gtttttcatt gcaacattga gaacattgca tcgttacaac atcaaagcat
     1621 ggatgtcatt accgtcatca actgtcccga tcgcgcgtgc gtcgaagaga aaatcgccgc
     1681 cgcgaaaaaa tttatgcgta ccgatgactg gatccatatt gacgtgaccg acggtatttt
     1741 ttcggcgcac gcgacatggc acgatcccgc ggcgttcgcg gcgatgggca ttccgttcaa
     1801 taaggaggtc catttgatgg tcgcgcgacc cgaagattat attgcggcat ggcgcgcggc
     1861 gggcgcgcaa cggatcatcg tgcacgttga aacgattacc gtcgcgtcgg cgcggaatat
     1921 cctggcggcg gccggcggcg aaaaaggaac cggcgcgacg gtgatgcttg cctcaaatcc
     1981 cgatacgccg attgaagcat taaatcctta tctcggttta ttctcggaat ttttggtgct
     2041 tgctgtccgt ccggggcctg cggggcaatc gtttgatccg tcggcactcg aaaaaataaa
     2101 gtttttaaaa cgcgaaggtg ctataatgat agaagtggac ggcggcatga atcccgaaac
     2161 ggccgcattg gtgaaagatg ccggcgccga tacgatcgca tcgggcgcct atattttcgg
     2221 aagcgccgat ccggcgaaag catatgaagc gctgcgggtt ctagagttct aatgttgtag
     2281 tgtcgtaata aagttcgcat gtcgttctca gcttggctga ggattcacgc tttctgtaaa
     2341 aataaagaat taaaatccat accatgaaag taattcttta tatggcgata acggtgaatg
     2401 gttttatcgc aaaggaggat gatgatgcca gttttatttc caaagaagaa tgggatagtt
     2461 atagcgccat ggtgcggacg gcaggcaatg ttattttggg ccatcggacg tatgatatca
     2521 ttacgaagca gccggaattc aaagagtttg agaatgtcaa agtcgtgata gtttccaata
     2581 aatcattcga gcctctcggt aaaaaacata gtattgccgg ttcgccaaaa gaagcactgg
     2641 gattattaag taatttcgag aatgttgtcg tggcgggagg tgcgattttg aacggttctt
     2701 ttttgaaaga tgatctcgtc gatgagatat atttggacat tgaaccaatt attctgagcc
     2761 aagggatatc gcttgtgaac ggatccgatt ttgaaaaacg tttgaaattg cttggcacga
     2821 aaaagatagc ggaaaatgag atacaattac attatcaagt cataaaaaaa tgaagagttg
     2881 taatgttgta gtgttatggt gagaatgcaa cgctctctgt cattacagta ttagaacatt
     2941 taggacacta caacatctat ggagcctctt accgaaaaaa aattgaaatc gctcgagttc
     3001 atggcgaatc gcctccgtga ggacgtgatt gccatggttt cgcgcgccgg ctccggccat
     3061 atcgcgggac cgcttggcat ggcggatgtt ttcgcggcgt tttatttcca tattttgaaa
     3121 cacgatccga agcatccggc gtggcccgag cgcgaccggc ttattctttc gaacggccat
     3181 atctgtcccg tgcagtatgc ggtgcttgcg cacgcgggat attttcccgt tgaagaattg
     3241 aggacgttgc gccggctcgg cacgcggttg cagggtcatc cgcatcgcgg cgctttgccg
     3301 ggcatcgaaa cgacatcggg gccgctggga tccggcatcg cgcagagcat cggcatcgcg
     3361 cttgcggcga aactggatgc caagaaatgg cagacctact gcctcacgtc cgacggcgag
     3421 cacgaagaag ggaacacgtg ggaggcggtc atgttcgcgg gcaaaaaccg cctgaataat
     3481 ctgaccgtgg tcattgaccg gaacaatatc cagatcgacg gcttcaccga gaacgtcatg
     3541 ccgctcgaac ctttgcgtga aaaatacgaa tcgttcaatt ggcacgtgct tgagattgac
     3601 gggcatagct tcgagcagat tatcgcggcg gccgccgagg cgcgggcgat ctatgaaaag
     3661 ccgacggcaa tcatcgcgca tacgattgcc ggcaaagggg ttgattttat ggagaaggat
     3721 tatttatggc attcaaaaac attcaaaccc ggggaggcgg aacatgcctt aaaggaactg
     3781 cggacattgg gcggcaagat aaaaagcgaa cacgaatgaa agatgtaata ttctcatggt
     3841 gtagtgttat agtgaaaata caacactgaa tcaacgctca gccatgagaa cattacaata
     3901 ttagaattag aacattataa cactacgacg catgcttaat cccgcattac atttaaatcc
     3961 gaaattattc gatgccgatg ccgaacagag gccgacgcgc gacggttacg gcgacggttt
     4021 ggtggctgcg ggcgatgcgg atcagaatgt cgtcgcgttg tgcgccgatc ttacggaatc
     4081 gacgcgcacc gaagcgttcg ccaaaaaatt tcccgggcgg ttttttgagc ttggcgttgc
     4141 ggagcagaat atggcgaccg tcgccgcggg tctgggtatc agcgggaaga tcccgttcat
     4201 cgcgtcgtac gcgacttttt ctccgggacg gaattgggaa cagatccgga cgacgattgc
     4261 gtacaatgat tcgaacgtga aaatcgcggg gcatcatgcc ggcatttccg tgggacccga
     4321 cggcgcaacg catcaggcta cggaagatat cgccattatg cggacgatgc cgaatatgaa
     4381 agtattcgtg ccgtgcgacg ccattgaagc gcggaaggcg accgaagccg ccgcgcatat
     4441 ctggggtccg gtttacctgc ggttccagcg cgaaaagacg ccggtcatga cgacgactga
     4501 aacgccttat gcgccgggga aagcggagat attctggatt ccgtcggccg gcattaaaaa
     4561 accggaagta tcgatcatcg tctgcggcgc tatcacttat aacgcgcttg ccgccgcgcg
     4621 cgaacttgaa aaggaaaaga tcggcgtgat cgtgctgaac tgccatacga taaagccgct
     4681 tgacgagaga aaaataatcg aatgcgtaaa aaaaaccggc gccgtcgtta cggtcgagga
     4741 gcatcagatt gcgggaggac ttggcggtgc ggttgcggag actctggcga aatatcatcc
     4801 ggtgccgatg gaattcgtcg gcatgcgcga cggttttggc gagtcgggcc agcccgccca
     4861 gcttcttgaa aaatataata tggacgtcaa gagcattgtc attgccgtta aaaaagctat
     4921 aaagagaaaa taacgctcct tttccaaaaa agaggctcct ccacggctca ttctcttctc
     4981 tagaggggga tctttttgaa aatacggcac ggcgcgaaaa gcgtcataaa tgccgtaaaa
     5041 aaacttttgt gaaaaaaata atgcgccgga cagcgcatta ttttaattat tgttcatttt
     5101 ttccgtccgg tagatcctgt ttttcccttc ttcttttgcc cggtagagca gtttatcggc
     5161 catatctata atttcctcga tgtttttccc ttcaataata tccgtcacgc cgccgctgag
     5221 ggttatgagc agaggggatt tttcttcgcc gacagcggcg taaaaaattt tctgcgcttg
     5281 ccccgtttct tcatcgtaaa atttttcgat aactttttcc gcatcggtgt cttgaaaaaa
     5341 gatgacgaat tcttctccgc cccagcgggc tacgatgtcg ttgtttcctc tggttatttt
     5401 ttgcagctct tctgcaatct ggcgcaagac ctcatcgccg gctgcgtgac cataggtatc
     5461 gtttattttc ttaaaatcat cgatatcgat aatcaaaacg gctcctggtt cgtgtttctg
     5521 ctctggatcg cgtctttctg gttcgggtgg ccatgtctta tcgatgatgc cgtttatctt
     5581 ttctaaaaat ccgtgtcgat tatccactcc cgtcaagaga tccttcgtag cgagtttgtt
     5641 tagccgttta ttttcctccg caaggcgctc tttatctgtt ttaagctgat tgttttccgt
     5701 tttcaattgt ccgttttccg cgttcaaccg cccgatttcc tgaatgaagg attccttcgt
     5761 ttcggtttcg gccggatttt ctatcgaatt ttgactttcg aatggttctt tctccatgct
     5821 taaatcatat tctattaatt tttttgtcaa atcgtcgctt tccggatgac gcggagatgt
     5881 ttttatgata ccataaagga aataaataaa ggaaataaat cctgcacaat tcaaaca
//
LOCUS       DAIDDT010000047         5815 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_47_length_5815_cov_6.010282, whole genome shotgun sequence.
ACCESSION   DAIDDT010000047 DAIDDT010000000
VERSION     DAIDDT010000047.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47_length_5815_cov_6.010282"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1471 a         1278 c         1521 g         1545 t
ORIGIN      
        1 atgataacat tataacaaat attttacatc aatggaaaat cataaaaaac attcgcttgg
       61 ccatcgcgcg ttcatttttt tcctttcaaa gcgcctcaag atcccgttct ttatgtttat
      121 acttgtggcg gtgttttggt ataccgaacg atggattccg tcgggtccat cgttttttat
      181 atggcagata gggcaggact atattacgaa gttttttctt cttatgagta tcgcgtatct
      241 tgttttagtg ctcgtgcgga cttacctgga atatcgctat tatacgtacg ttttcacgga
      301 ggaagcgttc gtgatgacgt ccggttatat tatgcggagc gaggttgcgg cgctgtacca
      361 tcagatccag aacgtgaata tcaatcgtgg cattctcgac cggctttcgg gcgtgagcca
      421 tgtcgtcatt tttatgacgg gttccgagag gggcaatacg cacaataaga tcgtcttgcc
      481 ggcagtaggg agagctaagg cgaaactggt acagaaggaa ttgctcgttc gcgcgcgcaa
      541 tcatatcgcg catgaatccc cgcggccgga gtagggaatc cgcataagat ttctttagaa
      601 cccgtctcaa aaatagcttt tataaaactc ccgtgggttt cgtggtgttt aatgaaattt
      661 tgttttgaaa aaaatcttcg cggtatttga tggcctattt gaggcaagtg tcgagtttta
      721 taaaaaagaa aagggctcgc cgcgtaaata tgcggagagc cctgtgaaaa aaatcgcacg
      781 atgcgatgga ttgaatggat caatatccgc ctcaacagag gcaaaaaata aaaatatatt
      841 ggtcgggcgt tcaaggttcc atttgctgcg ggacggacac ggacgccgag ccgtattctt
      901 cgaatgcgct gtcgataacg atcgcgcaga acctcacctc gtcgtcgagg aaaaagaaac
      961 acggtgcttc attaaacgtg ccattggcgc tacagctgaa ttcgtctcct gcgcagttga
     1021 tataaagatc atcgccgtgc ttgagacgtt cgggatgcgt gagaagcata atcccggtgg
     1081 cgaatacgcc gaggccggat tcgaaggtat tcataattat gcgtgcctta cgaatcgagc
     1141 ggcccctatg atgccatccg aactgagccg caatgacgag gaagtcataa ttcttctgtt
     1201 ctccgccgag tttccggaat attttttccg acttttcgga ttggcggaga tgtcgctgac
     1261 cgagctggcc atcacgatag ttgtagaatc tgccgttgcg agcctcctta atcctgtcga
     1321 gcaccatctg taccgcttcg ttgtaggttt tggccatcgt ctgccacttc gggacggcga
     1381 accatccttc ggtgttcggc ggaagcggcc gttcggcaat tgtctcgtcg gcgaaaccga
     1441 tgccggggaa aagctgatgt aagatgtttg tctgttcggt gatgcttttc ggtttgtagc
     1501 ctgagaagta tccataaccg gattccacct cttcttcttc ggcgaattgt gcttcttgtg
     1561 tcattactac ctcctctttt ttggaattac cggatgccct ccggttggag aaacgcctcg
     1621 atatgagacg agtattcaaa gggcttgcgc gacccgctga acactcatct catatacaga
     1681 cagataaaaa ccttgcattt ttttcagttg tcaaagagcg gatgtattat aactatgtat
     1741 agcagagtcg tcaatcgcgt tgcgcaaatt caatcttgaa ttgcattatt gacggcggcg
     1801 tttctgacga ctggattttc ggccgaaata cataatcaaa gcaacagatc tttcatgaag
     1861 gatctttttc cttgacaatc acagagcggc ggctatactt atgaagtaat tccaaagaca
     1921 gcaggttgtg ccggacacgg aaatcctgcc gaggaataaa cggaaggcgt tcgctttcgg
     1981 tttatttcaa cgacctttgg atgggtcgtt ttttaaataa tacggcgcga atccattgta
     2041 atccgcatta tccgtaccaa tatgaaaaca aaaattcaaa aaaccgaaga acttgcgcgg
     2101 gcgaaagagc ttttggaaaa gagccgggct ttgatattca ccgatttcac gaaagttccc
     2161 gcggaagacg tgcggcggct gcggcgggaa ctcaagaaat ccggcgcgca atttttggtc
     2221 atcaagaagc gccttttgtc cctgcttttg aaagagaagg ggattgccgc cgatcttaca
     2281 cagttcaaga cgtcggtcgg aaccgttttt tccgaaaatg acatcgagcg cgcgaccgcc
     2341 cccgcgttca agttcttttc cgcgcttgaa attcccgaag gatcgccgaa ggacatgtgg
     2401 atcaagcacc ttttgggcgg ctatgacatg gcgaagaaca gttcgttcga taccgcgcag
     2461 atcgttttca tcggaaagct cccgccgcgc gaagtgctgt tgtcccggct tttgggaacg
     2521 ctcgccggtc ccatcagatc gctgttatac gtgttggatg aaaaagtaaa aaggtcgagc
     2581 taacgcaagc attattattc caatggcaaa taatttagaa gatatcatta caaagataga
     2641 aaccttgagc gcggtggagc tcgcggagct cgtgcataag cttgaagaga agttcggcat
     2701 ctctgcggcg gctccggtcg cggcggcggg tgcggcggct ccggcggagg acgagaagac
     2761 cagctttaac gttcttttga aatcgagcgg cgaaagcaaa ttgaatgtca tcaaagtcgt
     2821 tcgtgaagtg acggcgcttg gcctcaagga agcgaaggat ctggtggatg ccgctccgaa
     2881 agtggtgaaa gagggactga agaaagagga agcggaagag ataaagaaaa aacttgaagc
     2941 tgccggcgcg acggtcgagc tgcagtaagg agaggaaatt attatccgag ccagctcttt
     3001 tttgtctgtt ttttttctgt catcctcgcg gaggcgatgg cagacccgca acaaaaccgt
     3061 cccgatgcgc atcgggacgg ttttgttttg tgttatactg tacactgata ttaaacaaaa
     3121 ggtcggttat cattttacgc tcatggaaga aataaaaaat caggaaaata aaagtttttg
     3181 gaggaaattc aacccgccgt tgaaagagct tgaggagcgg catccgaata tcagcatttt
     3241 ggaattgtgg tgggcgtggt attggcgatt ggcggtgctc gtgatcggcg tttgcgttat
     3301 tttcttcgtc attgcgctgg tactttcgtt gttcacgaaa taatttcata taggacttac
     3361 gcgtttgaaa aaatattttt aaaacgtcgg atgtttcaaa ccgcaacggc ttggctcgtt
     3421 ccttcattat tttcctgcgg ttttcatcgc cgtagtcgga ctacggctca gaaaatcctc
     3481 gggaaatgcc tcgtgcttgg ccaagcgcgt acgtcctata aaaaattgag attcatgcgc
     3541 atcattgcgc ttttaagcga cgatttcctt taatcgattg aagaaatcgt tattttaact
     3601 tatgctttta cagcaggtgt ttttaccgtt ttttcctgta tcgcttgcat tatggcgccg
     3661 ggcggaagga aaatttttaa ttgcgcatca tgccgcgtct ttagctcagc ggtagagcgt
     3721 tttgttcaca acccgcctgc cgaagcgtgc atagctcagt ggttagagcg ctccgttcac
     3781 atcggagagg tccctggttc gattccaggt gcgcgcacaa aatttcgcag cgggcaggtc
     3841 ggagaagccg caggtttgat ccccgtaaga cgcacccaat tattaaatga ctaaaaaaat
     3901 ggctaaaaat ttttccgata atttcagggc cgctttcagc gggttcaggg agacggtgat
     3961 tttcgaaaag agttttaaaa taatgctcgt cataaccgcg ctcgtggcgg ccgccatgtt
     4021 ttattttccc acttcgcgac tggaaaaaat aatactcctg ctcacgattt ttgccgtgct
     4081 tattctggag ctcatcaata cgaccgttga aaggattatg gacgtgatag tccccgaaca
     4141 cgacaacagg gtgagaatca tcaaagattt gatggccgcc atagtgctcc tcgcgagctg
     4201 cggcgccgct cttgtcggca ttttggtttt cctcccgtat attttgaatt tattcgcgca
     4261 ctgatgccgc cggcgtgcgg gcgcgcagga tgttttattc gaataatccc gaatttaagc
     4321 cggataaata agatacagcg aatagaagtt atataaaaaa taggcctcat tgctgaggcc
     4381 tataaaatca acagttagat agaagatgat actgaatgta gtataacata tccataattt
     4441 ctgtcacgca acacaagagc gcagttcgcg gatatgacgg gttccgggat atactgtatt
     4501 catgcaggaa tacgtgaccg aagcaatcgt tttgagaaag caggcggtgg gggattgcga
     4561 tttccgctat gctcttttta cgcgccgttt cgggaagatg gtggcgaaag cgaaaagcgc
     4621 gcggaaaatc gcatcgaagc tcgccgggca tctggagccg ggaaatatcg tacgtgcgcg
     4681 gctcatcgaa aggaacggct tgctgattgc ggatgcgctc aaagagacgc ggctcggcgt
     4741 atcgcttgcg gatctttctt ttttgtcgga tcttttacct gaaagagaag cggagccgga
     4801 tctttgggcg gcgtgcgccg ccggaaaatt cgcgcggcgc gatatccttc gcgcgcttgg
     4861 ctgggatccg gattatgccg catgcgccac atgcgattcc cgtgcaatat atttttttaa
     4921 tgtttccagt caggattttt tttgccggcg ctgcatcggg agctatctca agaacaagcc
     4981 gcgcgccgcc catatctatt ctttactata acaattagac attccatttt tgccgagctt
     5041 gattgaaccg gtgttttatc tctgagatga tgtagtataa tagtatgatg cgtaaaaaag
     5101 tggtgctgtt cgattttgac gggacgattg cggcgaccgt gcaggcgggt atcgatgtct
     5161 tcaatgaaat ggcgcgcgag cagggttttt cggaagtaac gccggacaac atcccgatgc
     5221 tcagggaaaa aggagtgcgg caagccgcgc aggaattggg gattccgaga ttaaaaatac
     5281 cgatggtcgt gagccggctg agaaaaggag tgcagtcgcg gattgccgcg atcgcaccca
     5341 tgcccggcat gaagcaaatt ctccttgcgc tcaagaagag cggctgtgaa ctcgggattg
     5401 tcacgtccaa ctcaaagagg aatgttctcg catttctcaa aaacaaccag ctggaaattt
     5461 tcgattacat caatgccggc agcggcgttt tttataaggc gcgcgcgata cggcatacgg
     5521 tcgaaagcaa cggcctcgaa gatcatgaga taattttcgt cggggatgag atcagggatg
     5581 ttgtcgcggc gaggaagaat aagatcacgg ttattgcggt cgcatgggaa actaattcga
     5641 aaaaaggatt ggaagaggcg ggcgccgatt tcgtcgccgg cacccccgaa gagcttgcgg
     5701 cgcttcttgc tgatttttcc gagatgacgt cggaagaaat ggaatacagt atttagtatt
     5761 tagaatatag aattctgaat tttacctact aaattctaaa tactatattc tatat
//
LOCUS       DAIDDT010000048         5445 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_48_length_5445_cov_6.194858, whole genome shotgun sequence.
ACCESSION   DAIDDT010000048 DAIDDT010000000
VERSION     DAIDDT010000048.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48_length_5445_cov_6.194858"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1538 a         1372 c         1304 g         1231 t
ORIGIN      
        1 cctctctgtt acggcaaacg caattaagtt ttcaagaaac tgtttctata gtagcacagg
       61 atatatattt gtcaaattta tttgcaaggg gtgatcgtaa aatgcgaaga aagcggaaat
      121 ggcttataat atccccatgc agtttcaagt tcctcaattc atcgagacgg aagataaagt
      181 cgtggggccg ctgaccatcc gccagttcat ttacgtatgc gcgggagccg ccctctgcgc
      241 gatccttttt tttacggtta cggggtggct tttcgcgatt ctttccgttt ttatcatggg
      301 gctgtccgtg gcgttcgcct tcattaaaat cgacggacgg ccgttcatca atatcgccct
      361 cgcggccttc ggattttatt ggaagccgca gatctatctt tggcagtcgg aacagcggac
      421 aaagaaagaa gaccatcccg gtctcggcgc cgcactagaa gacatcgccg cgggacttgc
      481 gctccataaa agctgggaga atctccaaac gaaagaaagg atttcgaacg tgcaaataac
      541 cgaccgttac caggtgttcc ggcggcagag cggcgaacag catgccgcga aaagagtgga
      601 ttataggtaa tggaaaaact gacgacctct ttttcccttt gtctgtattc gtcgtccccg
      661 cctacggttg aggacgacgg aaagaggacg ggcaaaaact acggatgaca ttccatgaaa
      721 actgtgcgaa aataaaaaga caacgaccat gcccatgcca tcaaaacagg attcggggaa
      781 ctcgctcgat cttgtcaata tcaaggaaat aaaagaaacc gccgtcatta tgaaggacgg
      841 aagcatgcac cagattatca tggtgggcgg cgtcaatttc gcgctcaaat cggaagatga
      901 acaaaatatc atcacgcagg ggtatcagaa cttcctgaac ggcattgcat atccgcttca
      961 gatcatcgtc cattcgcgca aagtcaatat cgggaaatac ctcgatacgc ttcgcgcgag
     1021 aaaagaagcc gagcaatcgc ccattcttca gaaccagata gacgaatata tcgattttat
     1081 cgacggcttc gtgcaaaaaa acgcgatcat ggaaaaaact ttcctcgtgg tggtgccgtt
     1141 ctaccctctg aacgcccttc cgaacaaaag tgccgtttcg ggcatgctgc cgttcttcgg
     1201 caataaaaag aaaaacgcga aagagacggc aaaaaatcag gaagaacagg aaggcgcgtt
     1261 cagggataat cttgtccagc ttacgcagcg gaccaatcag gttatcaacg ggcttctcac
     1321 tatcggcctc gacgcggaaa cgctgggcaa cgaacaatta atagaacttt tttataattt
     1381 ctataatccg cagaccatcg aacggaaaga aatggcgatc cagggcgcat tatcattata
     1441 atggtatcgc attctgccgt ccttgacctg gtcgaggatc cacgctttga aatttcataa
     1501 tgtatattca aaaaaaatta agaaaaggac tggaagcatg gatccttgcc tccgcgagga
     1561 cgacaaatac gggcgtatgc cagtaattat tatctattaa aaccatggat cggccgcaac
     1621 agcaagaaaa tttcgacgcc ataaaaaaaa tcatcgcgcc cgccggagcg caggtgttcc
     1681 cgaactatct caagatcggc gataaattcg cgaaaacctt ttttattctc tcttatccgc
     1741 gctacctttc gacgggatgg ttcgaagagg tcattaacct gccgaatctc ttcgatgttt
     1801 ccatttttgt gaaccccatc gacaccggca cggcgctcaa aaatctccgc aaaaaagcgg
     1861 gccagcttga atcgcagcaa aacgacaacc aggacaaagg cctggtgcgc gaccccctcc
     1921 ttgaaaccgc catccaggac gtcgataccc tgcgcgattc gcttcaacaa tccgaagaga
     1981 aacttttcga taccggcgtt tacatcacgc tctatgcgga taccgaagac gaactggcgc
     2041 ggctcgaaaa caagcttata agtatgatgg aggcgaaact tatttacata aaaccgactc
     2101 ttttcgaaca gctggaaggg cttttttcga tcctgccgac caacgaggat aaaatccagg
     2161 tctatacgcc gatgaacacc ggtcctattt cgtccttttt cccgttcgtc tcggaagatc
     2221 tcacatcgaa ccaggggatt atgtacggca tcaatctcta taataacaac ctcgtcatct
     2281 tcgaccgctt ctcgctcgaa aacgcgaacc aggtggtttt cgcaaaatcc ggctccggaa
     2341 aatcatacac gacgaagctc gagatcatcc gttcgctcat ggcgggtatc gaaatggtga
     2401 tcgtcatcga tccggaaaac gaatatcagc ggctcgcgga aacgttcggc ggcagtatgt
     2461 tcaatatctc gctttcgtcg catgagcaca tcaacccctt cgatctcccg ctcattccag
     2521 aagacgaatc gccgtccgac gtcctccggt cgcatatcgt gagtatcacc agcttgatca
     2581 agctcatgct cgggaaaatg tctcccgaag aggacgcgat tctcgaccgc gccatcacgg
     2641 aaacctacgc ttcgcgcgaa atcgtcgtcg acatggattt caccggcaag gaaccgccgc
     2701 ttttgggcga tcttgaaacc gtgctccgca atctcgaagg cggccgcagt ctggcggaaa
     2761 aactttataa atacaccaag ggaagttttg ccggattcct gaaccagccg accaatatcg
     2821 acgtttcgaa ccaccttatt attttttcca tccgcgatct cgaggaagag ctccggccga
     2881 tcggcatgtt cgtcgttttg aacttcatct ggagcctgat ccgcgcaaaa ctcgccaagc
     2941 ggcttctttt catcgacgaa gcgtggatta tgatgaaaaa cgaggacagt gcgacgttcc
     3001 ttttcgggct cgtgaagcgc gcaaggaaat attacctcgg cgtttccact atcacgcagg
     3061 acgttgagga cttcctccgc tcgccgtacg gccgccctat tatcacgaac tcgtccatcc
     3121 agcttcttct gaaacaggcg ccggcgacca tcgatatcgt cgtcaaagca ttcgatctca
     3181 caaatgccga aaggaacttc cttcttagcg ccgaagtcgg cacgggcatc ttctttgccg
     3241 gaaagaaaca tgtgacgata cagatcgtgc cgtcgtattt cgaggatcag atcatcacca
     3301 cgaaccccga acagctgctt gaagaaaaaa aagaagaggt cgcataggcg acaagcgaca
     3361 tggaacaagg aagagaggat tatattactt tccctgtcgt cctcccggag aggaggatcc
     3421 atgcttccaa tcttttcttt aacttcttaa agtgtgggtt ctcgccttat ggagcaggcg
     3481 aagccgccgc tccataaggc gagtctccgc gaggatgacg aaagggatgt tataaaaata
     3541 taaaggaaaa agctaatcca tggacgacgc gcaaaaaccc gaaccaaaga tctcgcttgt
     3601 tgaatttatt ttcctcgggc tttttattgt gcttatcgat ctcatcgacc tcgtcggact
     3661 tctcgtcggc ctgccgctct ccgacatcac cgacctcttc atgttcccta taacgcaatt
     3721 ttatcttttt atgaaaggcg tcaaaggaac ttatatgctt gtgggcaata tcattgaagc
     3781 gatcccgtat atcggcgacc ttccggttcg cacggtggtc tggtgtataa cttattacat
     3841 cgaccatcat cccaagcttg aagcgatggc caactttgcc ggaggcgcaa aaggaagctc
     3901 tgaagcggga gctatggcga gcgaagcaaa gaaagttgaa gaagtgggga aaaacgcgga
     3961 aaaaggcgcc gccacggaag tcggagaaaa agaaggaaca gcggaaacga aaggcaaaga
     4021 aacggaaaca gacaaaagga aagaagaagg tgaaaaaaga gaaactgaca agggagaaaa
     4081 agccggagaa gagaaaaaag aaattgcgcc cgaagcgctc ggcgaagaac gggaacctat
     4141 ggaaaagctc ggtcaagaac ttctcaatcc cacgcaagag caggaagaag cgcatgcggt
     4201 aaaagaagaa tcggaagccg gggaaacgtc gaaaaacaaa actgaatcgg tggaagatgc
     4261 cgagaaaaaa atgctgaaag cacggcaagt caaagaacaa ttagaaaagc tgaaaacgat
     4321 gccgacggca aatgataatg ataacaagga agataaagag gccgcataaa aattaagttt
     4381 gtgttatttg tgaggcggcg acaatagaga gagaaatcca aacaatcatc agggtaaccc
     4441 tttgctatct aaaccagcgc cgattccgtc gaaacatttt cccctccgtt gtccccttgt
     4501 cccctctgtc gtccccgcgg agactcgccc tatggagtga cgacttcgcc tactccatag
     4561 gacgaggatc catgtttccg atcttttctt aaatttcttt ttcttaaagc gcggatcctc
     4621 gaccggaacc tgccctcgac ctgatcgggg gtcgagggcg acggaaaaga ggggcgcgat
     4681 gggttacgca tcaaacaatg gactcttaaa aacaaaaaat ggtataataa agccatgaga
     4741 atctatcgtg cggtgttttt tgttttattt tcaatcacct ctttttttgc gctgtctttt
     4801 atttttctgg gaccggcgcg ggcgcagcag gcgacgccgc aattcctgtt cacgtggagc
     4861 gcgtcgaaca gctatgttcc gccttcttat aagggaaaag ttttgcctga acagcaatcg
     4921 aaaatcacgg catcattcga actggtttcg ggcggaaaga tagtgaatat cgagaatcag
     4981 cccgtctatt ggtaccttga cgacacgttg atcggcggcg gcgcgggaat gcaacagata
     5041 acattttctc cgttcggaat ggcgccggaa atggaaactt taaaagcgga attgccgaat
     5101 tatcccggcg gttatctcat ccatcaggcg accattcccg tcatcaatcc cgccgcggtc
     5161 attgaagccc cttatcccaa cggccaattt tccggcacga gcgccacggt cacggcactc
     5221 ccatatttct tcagtgttcc cgcaaatgat ctttctttca catggtcagt gaacggacaa
     5281 tcaggatcaa acgccgaaaa tcctgatgtt ctcgacatta atcttcccac gggaacgcct
     5341 tccggatcga cgcttgcggt ttcgctgcag gcgcagaata cgaacgacgg gcagagcgcg
     5401 agcgtatcga ccaatcttgt ttatcaacaa cagttatagt gaatc
//
LOCUS       DAIDDT010000049         5429 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_49_length_5429_cov_6.515321, whole genome shotgun sequence.
ACCESSION   DAIDDT010000049 DAIDDT010000000
VERSION     DAIDDT010000049.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49_length_5429_cov_6.515321"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1240 a         1525 c         1325 g         1339 t
ORIGIN      
        1 attttgactc ctctttgctt ttgaatttta tattccgaat tctctattct gattgcacga
       61 ggcctgggcg ggaatcgcac ccgcgtataa gggttttgca gacccttgcc ttactacttg
      121 gctaccaggc cattgagaaa ccggttccgg ccgctttttg agtttaaaga aaaaaacgga
      181 tatcttcaag cgaataaggt tgtattcaaa aacctttgaa aaatccgctc ttctttttcc
      241 tcagccgagg acctacgctt taagagattg aaaagaaaag attgaaagca tggatcctcg
      301 ccccatggag tggcggcttc gcctactcca tagggcgagg acgacccata aaaacatttg
      361 ttctctctcc aaattctact ccaactccaa aaatttcttt tccacctgcg cggcgtttgc
      421 cacgccgcgg tcgattgcga acgcaatccg cggcatcggc ttgatgttta ttttttttaa
      481 aagcgcgtgc tgaagctggc cgatattctt attgagcgcc tgcagggcgg atttttcttt
      541 tccggacggg atcacgctca acagcacttt cgcgtggtca agttttttat ctgcttcgac
      601 ttcggtaatc gtaatcagcg cgccgggaaa ctcaaaatcc cgcgcgagaa ttttcgataa
      661 ttcctcgcgg atcaatttct cgacgcgctg tgaacggtaa aaacgcataa tgggtaaata
      721 aaatatttaa atatatgtct ctgctttcgc cgatgcgagg acgatgcaaa atgtcgctta
      781 ctttctgata accagaatgt cgccgacttc gatcattatc ggcgcactcg cgagaatacc
      841 gatctctttc cctttttccg cctgggttat ttccgatttg ttttcgcgca atgtaagaat
      901 tttcccgaga ccgatcggtt cctgcgatcc cgcgccgcgg aaaatttcaa acggcacttt
      961 tgcgcggaaa acgccgctcg atatccgtcc gccaaaaagc tgcttgtccg atttttcctg
     1021 attgaaaacc gcgagcacgt cgagcgttcc cagggcggtc gccattcccg cgcccgtcaa
     1081 aagatcttcg accgctttca cgatatcata aaccactttc gacgcgatga ccggcacgtg
     1141 ctgcgcttcc acaagattct gcgcgccttt ctcgatgcga ttcttgaaag cgacaatgat
     1201 ggcattcgtc gcggcggcgg tccgcacatc gccgtccgta acgtcgccga cggattcccg
     1261 gactatgttt attgtttttt gcctttcaac ggcgataccg cgcaacacca tgccgagcgc
     1321 ttcgagcgat cccgcgtcgg atgctttgag tatcaaggca agcgcattcg catcgcgctg
     1381 cggcatatct ttcgtttttg attcagaaat atcttttttt ctgaacgccg cactatccgt
     1441 cgaaaattcc tctccgatcc ccggcaatga ttcaaaaccg atgacgagcg ccggcgcgga
     1501 tggcgataag cggtccgccg gatttcccat aaagtcctcg agaattttaa tttttcccgc
     1561 ggcggaaggc gtcgcaatat cgtcattcct tttcagcgtg ccgttcttca cgataaccga
     1621 cgcttcaatg ccgcgccgcg gatcgcgccg cgcctcgaga atgtatcccg acgccggaac
     1681 atccggatcg tatgcaaggc cctccatgtc cgccgcaagg ataatcaaat ccaaaagctc
     1741 ggcgacgccc tcccctgtct tcgccgaaat gccgtgatag ctcacctgac cgccgaagcc
     1801 ctccaataaa acgccggccg ccaaaagatc gtttttcgct ttgtccatgc tcccgttcgt
     1861 gcggtcgatt ttattgacgg cgaccacgaa tggcgtcccg gtttcttcca gtatttttat
     1921 cgcttccttt gtttgcggct tcacgccttc atcggcggcg acgacaagaa cggcaagatc
     1981 ggcggcatgt gctccgcgcg accgcatcgc gctgaacgct tcgtgcccgg gcgtatcgat
     2041 aaatgtaatc gtgcgatcgt tatggacaat ctcatatgcg ccgactgcct gcgtgatgcc
     2101 gcccgcttcc cgctcggcaa ccgaacgcgg cgcgacctcc gaaatttttc cttgaaattt
     2161 ttttgagatc gcgccgcgtt gtacggcacc gcgtgcggcg tacgtcatct tccggatatg
     2221 gtccaaaagc gtcgtcttgc cgtgatcgac gtgtcccatc acggccacga tcggcggccg
     2281 cggttgcagt tttttcgttt tgtcaggcat aatctttgta atttaccaca aaaatcgaaa
     2341 aaaaacaaca atttttcttg caaagaagcg gttcattcat taaactgttc agtacggaga
     2401 ggtggctgag tggccgaaag cgcctcactg ctaacgaggt agggccgtta aacgccctcg
     2461 agggttcgaa tccctccctc tccgcaattg cagcaaatcg cccttatctg ttatgtcagc
     2521 aatgcgctga tatgcttttt tgtattgaaa atcctgtact ttttgactgc tcaccaaaac
     2581 ggagatgaaa ccctaagaca taagtaagta agagtaagtt aagttaagta tagagtaaaa
     2641 atagcacgta aaaaagaaaa cggctataat gagaattaaa aagaagtaag aatattggca
     2701 ataaaaggcg gcggttatca tatactcaaa tggatccata tgcaggatta aaagatgcgg
     2761 acatcgccgg attgcgggcg cactacggct tcaacgaaac gcccgttttc cacgagagca
     2821 tggtcgtgct gtacctcaag aatttttggg gaccgctcgc gtggctcatg gaagcgatgg
     2881 tcgttattac ttttttatcg ggcgacgctt ttgaagcgat cgttatcgcc tgcctgctcc
     2941 tcgtaaatag cggcatcaat atctatcagc gcaggtccgc cgacgccgct ctcgcgatat
     3001 tggcgcaggc gatccagacg accgcccgcg tacagcgaaa cggaacctgg agcgtcatac
     3061 catcacgcga attgttgccc ggagacagca tccgattgcg cgcgggcgat atcattcccg
     3121 ccgacgcaca gctttttgac ggaagcctga gcgttgatct ctccaccctg accggagaat
     3181 ccctgccgcg cgatatcaac gcgccggacg cgatctattc gggcggcatc gtacaacacg
     3241 gcgaagcgac cgcgaaagtg tccgcgatcg gcagcaagac ccggtacgga aaaacgaccg
     3301 aactccttga aaccgcgcat ccgccgacgc atatggagaa ggtcgttttt gacatcatta
     3361 aatatttttt cgcgctcaat gtgatcgtgg cgattgccgc ggtcattttc ggacttgccg
     3421 tccatgcgcc ggcattgcag atcacgaact tcgttatcgt ccttctcatt atgtccgtac
     3481 cggtggcatt tcccacgatg ttcgccgttg cgcaaaccta cggcgcgctc cagctcaata
     3541 aaggcggcga cagcaaagaa aacgaaggtt cgaaagtcct ggtccgccga ttggccgcgg
     3601 tgcaggaggg cgccattatg gacgttctct gtagcgacaa aacaggcacc ttgactcaaa
     3661 atcgcctgag cgtagcggaa gtcactcatt acggcgtgaa cgacgaagcc agggtactga
     3721 tgcttgccgc cgcgtgcagt aatatggcgg acaaagattc caccatcgat caagctattt
     3781 tccagaaagc agccgagctg aatgtcgccc ttttggaacc gatcagtttt tccccctttg
     3841 attcattgac caagcggacg cgggccgaga tcgttgagca agggaaaaaa atcaatattg
     3901 aaatgggact ggcggatctc ctactgaccc ccgcggtatt ttcttccaag gaagcattcg
     3961 cggacgttgt ccttatgagc ggcaaaggat tgcgcatctt ggcgattgtg acgagcgacc
     4021 cggacgcggc atgcgcgggc cttatcggtt tgagcgaccc cattcgtccc gatgcgccga
     4081 cgcttatccg ggaattgaac gatctcggcg tgcgcgtcgt catgatcacg ggcgacggcc
     4141 gcatcacggc gcaggccgtc gcgagccagc tcggattgca gggcgacgtc tgcactcccg
     4201 atgatcttaa aaataatccg gagatcgctc tgcggggcgc ggtcttcgcg gaggcctacc
     4261 cggaagacaa agtgacgatc atcaaagcgc tgcagcacgc gggacatgtc gtcggcatga
     4321 cgggtgacgg cgtgaacgac gcgccggcgc tccatcaggc ggagatcggc atcgccgtca
     4381 aggacgcaac cgaagtcgcg aagcaggccg caagcttcat tcttaccacg ccgggacttg
     4441 aaggcgtgcg ccgcgtcgtc acggcaagcc gccgcgttta catgcggatc cgcacgtggg
     4501 ccttgaataa ggtagtaaaa tccattgaga tattattcat cgccacaatc atattcgtta
     4561 tcacgcactc gtatatcctc tcgccgctca tcgccatcct cgtcctgctg gccaatgatt
     4621 ttttgaccat caccatcgcg accgatcaca cgaaaccgct tctccgtccc gcgcgatgga
     4681 acatcccgcg gatgattgcc gcatcgtcaa tgatcgccgc cgcgccattc ttctttacaa
     4741 tctccatcta cgccatagct caacattttc actatccttt tgataccata agaacaatcg
     4801 tgtacggctc gctcatctat ctcggcggaa cgaccctgct cgccatccgc gcatggccgt
     4861 tcggttggag catccgtccg agcaaaacgc tcctcggcgc gctcctgttc tccctggtat
     4921 tcgcctccat cgtcgccggg ttcggcatct tcatcacggc catgccgccg gtattctttg
     4981 cgctgattat tgcgggcgcg atcgtcagtt ttttcctgat tgaatccgtg aaacaatcac
     5041 acggcgtgcg cgcgttattg gatattgaat gaagctttcc gcggcaaagc cggcagaata
     5101 tcagaaaatc atgtcggttt tgtcgcagag aacttcatta taaatattta aaaacccgct
     5161 tggacatgcc aagcggggaa aataattatc tctttcttct ttctttcgct tcacttctcc
     5221 cggtgaaaaa aggaagtgaa gcctctgttc cctctatttt ctttttctta tgctttctct
     5281 ttcccaacag gctgcaaatc tcacgataaa tcgccgtccg ttcaacagga tcgtgaacac
     5341 gacattgatc gagcgctttt ccgaccgcag cctgaaattt attccatccc gataaaacaa
     5401 ggctgcaatt tcagcgatac tttcaatat
//
LOCUS       DAIDDT010000050         5254 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_50_length_5254_cov_5.090400, whole genome shotgun sequence.
ACCESSION   DAIDDT010000050 DAIDDT010000000
VERSION     DAIDDT010000050.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50_length_5254_cov_5.090400"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1414 a         1226 c         1385 g         1229 t
ORIGIN      
        1 cttcattccc gccctgatgg cgagaatgaa gccaaagata gaaatgcgtt ttcctgaaat
       61 gataagaatg ttgcataacg aagagggggg gataaacgcc tctcttttcc tgccgcgttt
      121 tttttgttac catcaatggg atggatcccg ttagaaaatt aaaaattatg gtattcttat
      181 actcgttatt taatttcgca actgactgct ttatgccgag aaacgtttat ttaattttct
      241 aacgggatga acattccgga aataaaaaaa tcttttttca gggaactcgg caaggcgcat
      301 gatgccgttg atattgagaa cgtgcgcgtg gcgtatacgg gacggaacgg aaaattaacg
      361 gcgatattgc ggtcgctcgg tattatgccg cttgaagaac ggaaaaagac tggcccttcg
      421 gcgcaggcat tgcggaagga aatggaagag gcgcttaagg agaaaacgaa tgcgctggcc
      481 ggttccggcg gacgaccagc atcaaatgtc gatctcaccg cgccgggaaa aaagttcagc
      541 cgcggccatc tgcatccgct tacgatagcc ctccgcgacg tccggcggat tttttcatcg
      601 atgaattttt cggtcatcga cgggccggag atcgaatcgg aatactataa tttcgatgcg
      661 ctgaatgtcg cgtcaaacca tcccgcgcgg gacatgtggg atacgttctg gatcaaacag
      721 ccggaaccgg aggaaaaaga tccgcggaaa catcttttgc ttcgtacgca cacgtcgccc
      781 gtccaggcgc ggtttatgcg ggaacatacg ccgccgttcc agatcatcgt gccgggccgc
      841 gtcttcaggt acgaggcgac ggacgccggc catgagatca atttcaacca gatagaaggg
      901 ttggtggtcg gcaaaaacgc gaatctcgcc aatttcaaat tcattctcga agattttttc
      961 aggaaatttt tcaaaggcaa aaagatcgaa ttccggtatc gtccgagcta tttcccgttc
     1021 accgaaccga gcgtggaggt ggatatcaag cttgcgggcg gaaaatggct tgaagtgatg
     1081 ggcgccggca tggtgcatcc gcgcgtgttc gagtacgcgg gctacaaccc gcgggactgg
     1141 cagggattcg cgttcggcat gggacttgac cggcttacga tgataaaata cggtattccc
     1201 gacatcaggt ttttttattc cggcgacctg cggtttataa aacaattttg attttggcgt
     1261 ggcgccgctc cggcatcggc gccggcaccg cggaaaaaaa cataaaaaat ctgttaaaat
     1321 ttatccatat ttctcttggt cgtcctcgcc ctggccgagg atctatgctt taagaaatca
     1381 acagaaaaaa ttggaagcgt agatactcct cttcgggagg acgacgaaag ggaaggcata
     1441 tatctttata ataaccatga aattctcata ttctctcgtc aagcgtcttt tacccggcat
     1501 tcctccggcg aaaaaacttg cggatcaatt aaatggccat tcatttgagg tggaggcggt
     1561 cgaaggggat atgctcgata taaaaattcc ggcgaaccag tattccgcaa tggcgtcgca
     1621 tagcggtatc gcgcgcgagg cgtcggcgct cttcaaactg ccgttcaaaa agccggcgca
     1681 aagaattata aacgctccgg tgcggaaagg attcgtgaaa gtgaaaatag acgaaccggc
     1741 cttatgcacg aagtatgccg cgcgggtttt cgacgtgaaa catatcggcg atgtaccctc
     1801 ccgaatttta tcggataaaa ctccggagga cgcgaccgcg gaaacaaaaa aaatactcca
     1861 cacctgcggt atcaatcccg tgaacgcggt tgtggacatt atgaatctcg tcatgctcga
     1921 aacaggacag ccgctgcacg cattcgatgc ggaaaagata aaaggaagtc tccgcgtgcg
     1981 ccgcgcgaaa aaaggagaaa cgatcgaaac gctggacaag aaaacggtcg cactcgatcc
     2041 ttccgtgctc gtgattgcgg acgatgaacg cgcgcaggcg atcgccggca ttaaaggggg
     2101 attgtcttcc ggcgtcacgc aggcgacccg gcgcatcatc gtcgaagcgg cgaatttcga
     2161 cggcgtcgcg atctaccggt cgtcgcgcgc gctgaaactc gtgaccgacg cgagcgtccg
     2221 tttcgcgcac ggcatttcgc ccgcgcttgt tcctgtcggt cttgaccgcg cgaccgagtt
     2281 tttggtaaaa aatggcgcac ggcttatcga ttccgtagaa gcgggaaatg caaaaatagg
     2341 ggatacgatc atcgatttcg atccggcggg attcgagcgc ctgatcggca ttcccgctcc
     2401 gatcgcggac gtcaaacgtt atttcgccgc gcttggattt acctttcaga atttttctcg
     2461 gaaaaatttt ccgaaaaatt caggaaagcg aagttctgaa acagcgccgc aaaaaagaaa
     2521 cgccgcgctc cgcgtgcgga ttcccgcatg gcggaatgac atcgagaatc cggaggatct
     2581 ctacgaagaa gccgcgcggc ttatgggata cgataagctg ccgaagcatc cgcccgtcct
     2641 taccatccag ccggcgcacg ttgatgattc gcttgtcctt aacgacaaga tccgcgatat
     2701 cctttccggg cttgcgatcg acgaagtcta taataattca ttttatggtc ccgccgcggc
     2761 ggcacatgaa aaattttcac ttatgtttaa aaaagacgcc gcgcgcgccg aggttctgaa
     2821 tcccgtttcg gaagatttga aattcctccg aaaaagcact ctgccgcttc tttttgaaaa
     2881 ttttgaaagc aacgcccgtt atttcgagaa tgtgcgttta tttgagatcg gaaaagtttt
     2941 cggtacggcc gcccgcgggc cggaagaaaa atcggcgctc ggaattttta tcgccgcaaa
     3001 aaaagaccgg cggctcgcgc tcgaactcaa aggcgctacg gatgaacttt taggaggact
     3061 gggtatcggt gatttttcgt tcgtgccgga ggatcatggt aatgaaatcc gtatcgaaac
     3121 ggaccatgcc gttctgggaa tgataggaac ggaaacgctg agacacggat gggagtgcgc
     3181 gtacgcggaa ttcgacctgg aaaaaatatc cttgttgacc gaagaaggga aggagttcgt
     3241 tccgctcaag cggtttccgg cggtcacgcg ggatgtttcc gttcttgtcg gacgcgacgc
     3301 gcgcatcggg agcgtcattg ccgcaatcga agccgcaaat cccgcgctta tcgagaccgt
     3361 cgagctgatt gacgaatata aagacgagaa actcggcggg aagcaaagca tcacgttccg
     3421 tattattttt caggcggatg accggacgct taccgatgaa gaagtgaatg atgagatgaa
     3481 aaaaatctca caggcgttac gcgcaaagtt tagcgccgag ataagataga aagataataa
     3541 attcacggga caccttttcc ttttccccag cgcttttatc aaaacaattg ttttcataaa
     3601 agcgctgggg ggtatccggc gtccgccgta atttttcgga aaaaaacaaa aaaaccgggc
     3661 gggctcaccg gcgcgatgat gaccgatgga tggatggctg gatacaaata atttgctttt
     3721 tataaaatat ctgctacact acattgacta tggagctcac ggtgcaaaca cgcgaaaaat
     3781 tcggcgcaac ggcctcgaaa gcggcgagga aagaaggcct gatccctgcg gaactgtacg
     3841 gacacggcat aacgaacggc catttttcgg ttgccgcaaa agatttcaca aaagcattca
     3901 aggaagcagg gacaagcact gttttatatc ttcttatcga cggagaaaaa ggttcaccag
     3961 cgaagaaaca ggaaaaaaag cccgctatta ttcatagcgt cgcgcacgat ccgcttacgg
     4021 gcgatatcgc gcatgtcgat ttttatgcgg tgcgcatgga cgaaatgatc acggcaaaga
     4081 taccgctgaa gttcgtgaac gaagcgcctg ccgtgagaga gaaggcggcc attatcaata
     4141 aatcgatggc ggaaatcgag gttgaggcct tgccgcagga tctgccccat gaattcacga
     4201 tcgacctttc gatgcttgac gatctcgata aaagcattta tgtgcgcgat atcaaggttc
     4261 ccaaaggagt gaaacttctt gtcgaagggg ataccgccat cgcgaccgcg acaccgccgc
     4321 ttgccgaaga agtggtcgaa gcggctccgg ccgatattga tattaccgac gtgaaagtgg
     4381 aaggcgaaga gaaaaaagcg gagcgcgaaa aacagaacgc ggaaaaaaat tagcattcag
     4441 cggataaaaa ccccaccggt aaggatgggg tttttattgt ccatcggatt aaattaaata
     4501 tggtataatt aaagcaatga aaaggggata tcaaaaagcg ctcataacgg gattatgggc
     4561 tttcgcggcg gttgcggtct tttattgcgg ctccgcattc gcgtatccat tggcgggaac
     4621 tcaggatcaa aatcaagcga cggaaacatc gttacaggtg ccgtcgttca acttcaactg
     4681 ggctagcggt atttttaata atttatccgc gccgttccag atgtttgtaa gtggtatcat
     4741 cagcggcata gcaccgtcaa aaacttccac aagtacgtcg aatatttcca caaacccggt
     4801 gaataaagtc ataaccgccg cgccgggtgt gtcgataggc aatattaatg gttatttccg
     4861 gcaatggacg accgtaagcg gcgcattatc gggtttcaat aatctgctct ccggcatttt
     4921 cgggttccgt acaacatcgt tctttagtgc cattacgggc gtgatagtat gggtgctgat
     4981 ccttgcaaaa agcattatcg actggctttt gtcggtaata cactgaaaga gtggcgagcc
     5041 cgaacttatt ttttatttat gatgtttttg tcgtttgatc ctacgttccc gccatttacg
     5101 tttacgacag tatatgaaaa cttttcactg acagtttccc agtttccggc cttttttatg
     5161 agaaatgggt acgtcatgtc gtaaaatgta tattgtgcga cagataggat attgataaac
     5221 tgaataatcc ccttaaaata aggaagggta agag
//
LOCUS       DAIDDT010000051         5164 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_51_length_5164_cov_5.526440, whole genome shotgun sequence.
ACCESSION   DAIDDT010000051 DAIDDT010000000
VERSION     DAIDDT010000051.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51_length_5164_cov_5.526440"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1350 a         1351 c         1211 g         1252 t
ORIGIN      
        1 gcccgaatat aggacttccg atccgtctca ctattccaaa aatcaatgtc gataccgcta
       61 tcgaggatgt gggtcttacg ccacagggat ctatggctgt gccgaagggt ccgacggaca
      121 cggcatggtt cgatctcggg ccgcgtcccg gggataacgg cagtgccgtg attgccggac
      181 atgaaggatg gaaagacggt atatgggcgg tattcgataa tttatataaa ttacacccgg
      241 gagacgaaat atatgtcgaa gacgaaaaag gaacgaccat cgcttttgtg gtgcgcaaaa
      301 ttcagacgta tgaccaaaat ggaaatgcct cggatatatt taactcaaac gacgggaaag
      361 cgcatcttaa tctcattaca tgcgaaggaa cgtggaatgc ggctcaaaaa agttattcca
      421 accggctggt ggtattcgcc gacaaggaat aaggcgcgga gaataacgga tctcttcaat
      481 ctcgcacagt gcggtgccct cgcgtgaaat cgttgcctga atttatgatc ctcaaaaagg
      541 tgatgaagcc gaagtttatc ttaatatttg cgggcatcgt ggccgtggga atcattttaa
      601 caggatatct ttttaatgcg atattataaa acaaatgaat aacgaactat ttgtagatat
      661 aaggacggac gaggaacggg atgacggcca tctggatagc gcattacatt tcgagttagt
      721 gcgattgaat cagggcgagt tgctgacatc aaaaaagata cactctcttc tggataggcg
      781 ttaaagtggg cgagttcaga accgctctcg gctattacgg atacggaata tggcctggta
      841 tgatgggaag tagttactac ggtgccaact tttatcctta tggctatacg ccgatgatga
      901 gcggatacta tcaaagccag aatgcggctc cgtcccaagc aatcgcgacg actaccccaa
      961 gctactacta cgagcataga ggtatgatgc aatgttatta tcagaacgtt ccgcaagcaa
     1021 ccggctcaaa gtaatcaaca ataaaatacc cgacaagcgg gtattttatt ttactttcac
     1081 aaaagggcta tctcgccaaa caggttctga atcccttcat taaagtattc tcggaggata
     1141 ctcttagaat atgaaagatc tctttcttgt tccgattcca atcatggtac accttgatga
     1201 aactcaaaaa agtcagcatt tacgccctcg gtcgatctta ctgtcttggt gcccccgtga
     1261 ggaattgaac ctcaattttc tccttcggag ggagatatcc tatccgttga acgacgaggg
     1321 cgtcgggtat gcgaaatacc ggatgataaa aaagatttac cggcgggcgg cgcagtatac
     1381 ggaaatggaa tacgacgtac cgcacaacgt tttccggcgc gtttccaacc gttatcacct
     1441 tactataatt cacctcaaaa atccataaac tgagtaaact gccgcgaaaa taaaaaccgc
     1501 cgatgcgatg ccgaaaaata ttttttgcgc gggtgaaaac gcgagcgaac gcgcgccgac
     1561 ggcgataata agaagatatt gcacgctcag aaaaagccct ccggcaatgc ttacgactgc
     1621 caaaaaatta ttaaatcccg aaagaacggc cgcgagcggc gcaaagacga ttgcggaacg
     1681 cgcaaaaacg ggatgccatc ccatgtcttt ttcgaacgca ttgcgcaatt cgcgcatgat
     1741 aggcatcgct atcgttccaa tcgcaagcaa tccgataatc gcgataatat cttttttcca
     1801 cgccggccag tttgtcaatc ccgacacggt gtcctgcgtg atcagcgacg ccgatccggc
     1861 aatccccatc gcaaataaaa aataaagcag cgccgcaaaa agggttccgg cgataaaagc
     1921 aatccataaa atactgcgac ggccggtttt taaaaaagac cctggttttt gcattacgcc
     1981 ataaacggat tcaacgctcg tccatccggc aagcgaaaaa agaacggcgc cgagcggaag
     2041 gaaaagataa tggagggcga ctggcggaat gacggataat gcgtggacgg gatttcccgt
     2101 caaaaaaata aaaagaacga ccgcggcaat gcagaaaatg ccgaattctt caagatgaac
     2161 cgtgcgctca tcgccaagaa aaaccggtat ggcgatgacg aaccaaaata aaacaagcgc
     2221 ggcagccggc ggcacggcgg gaaaaataag tcgtaaaaat tgcgggccga tgatgagaaa
     2281 cgccacaatg cccatcaata aaccgatgac gatggcgatg aatcccgtca aaaatcctgc
     2341 cgttccgaaa tacttccgcg cgagccccag gagccgctct ttttcaccga ctgccgcaag
     2401 cgtcttgaaa taaacgatat gcgccgctga aataattgcg gcaaacacga cgaaatagca
     2461 ggccgcaagg atccagcccg cgcttgcgac gatatacggc aacgcaaaca cgccggttcc
     2521 tatcgtcgcc gccgccacga tcgccgcgat ctttaaaaat tgacggaagg cagccataga
     2581 aaattctatc ccgccttata cacctcatcc ccttcgcgga ctttcttttt tactttaatg
     2641 ccgatctgtt tccccttcgg cgccgcggca atcgaaacat ggttatattg cattgatttc
     2701 gccgtttccg tgaaatcggt cgttgcgccc ttgaaatgca gcgcggttcc cgcaggaact
     2761 ttttgattga acttcacgat cgcgacacgg atattgccga aaaaatgcgt caccgttccg
     2821 atcggctttg tcttcacgga tttctgagcg cgaaccggtt tcgcggcatt ttttttcgcc
     2881 ggcttccgca aaaacacagc tttcggtttc gcctttttcg cttttatttt cttgaccagc
     2941 cgtttcgctt tcattatctt cttcgccttt ttcgccttaa cggatttttt ccgcgtctgt
     3001 gtttttattt tcaccgcttt ttttgttttc ataataattt taaaaaacgt cccgatgtta
     3061 tttattgcac tatttcaatt tcattgaccg ccgtaatgga agtaaaaact attcaatgtc
     3121 gaactcgctt tcttcttttt ttagcaatgc ggcaaccttt tcaatcagcg ctttcgggtc
     3181 ggtgtcaaag acgacttttc ccgtaccgcg atgagcgttt ttcagaatat cctggatcat
     3241 atcagtcgta ccgccgatgc cgagcaagac accctgcggc ttgccgtcct cgaatgcatc
     3301 cgtaaattca ttcaaagtgc caattctgcc gcagaccgtg ataacggcat cggcggaacg
     3361 ggtgaaaaga agattccgtc cggaatagcc aaaaccggta tagacgatga gatcgtgata
     3421 atcgagcgga agccccattt tcacgtgttc gcgtttcgtc gccgccggcg aaatgccgat
     3481 cacgataccc cccgccagct tcgcgccctt cgccgcccaa tacggcgcgc cggtcgtcgc
     3541 tcccgtcacg gcaaccatgc cgcgttccgc gataagccgc ccgaccgttt caactttttc
     3601 caacgcatca ggagccatga attccgtttc cgccgctccg gaaaccgcga tcttatattt
     3661 caggtgatac gccccgaccg ttgttgcagc cgtttcctct tttttattga atgcgtccat
     3721 aataatgaat ggttaaattt atttaaaacg aaactttaca actcaaagtt cgacttcttc
     3781 ctcaggttgc ggcacttccg cggcgacggc gaattcgtcg cggatctttc cggcaagaac
     3841 ttcactcgaa gacggatcgc cctgcaccac gaaaactttt tttaatgtgc tcttcatcgc
     3901 gccaacccat tccaaaagcc gcggctggtc ggcgtgggcg gaatatccat ggatggccgt
     3961 ttttttgcat cgcacgggaa cggtctcgcc gaaaatcttc acctcggccg cgccgtcctg
     4021 aatttccctc ccgagtgttc ccgctgcctg atagccgaca aaaagaatcg tgcttttcgg
     4081 atcggggaaa taacgcagtt catgatggag gatccttccg ccgttcgaca tgccgctgcc
     4141 cgcgatgatg atcttcggcg ccggcacgtt attgatcgcc ttcgactggt cggtggtcaa
     4201 agtaaaatgc atgcccggaa aactcaaaat atcgtcgccc ttttttatga tgttctcggt
     4261 ttccgtattg aaataatttt catgtttttt atagacctcg gtgagcttga tcgcaagcgg
     4321 actgtcgatg aaaaccggca cgcgcggaat gcggccttcc tcgaaaagct gatgaagatg
     4381 gtaaagcagt tcctgcgtgc gttccatcgc aaacgcggga atcaggagcg tgccgccggc
     4441 tttcacggta tcctcgacga catcctccaa aagatccttg cgccggtcca tatcctcgtg
     4501 cacacggtcg ccgtacgccg attcgacgag gcaataatcc gcgccgctca tcatttcggt
     4561 cggacggatg atgggcgcgg ggaaattgcc gaggtctccc gaaaaaacaa cggttttccc
     4621 ctccgcctcg acgtttatga ttgcggatcc taaaatatgc cccgcatcga aaaacgtaac
     4681 tttataaccg cctatcgcca ccgtctcatg gtaatcgatt ccccgccaga aggtcataag
     4741 caaatcgata tcttcgccgg tataaagcgg cggcttcccc tcccgcgccg cctcgttgcc
     4801 gagaatatgt tcggagtccg taagcatcag ccgcgcgaag tccttcgtcg ccgccgtgga
     4861 ataaatcacg cccttgaaac cgttcttgac gagcttcggc agaagcccga tatggtcgag
     4921 atgcgaatgc gttatgaaaa ccgccgcgat ctcctcggga cggaacggaa acggctcgaa
     4981 atttttcttc tcggcgtaat gccctccctg atgaagcccg caatcgatca taatcttcat
     5041 cccgtccgac tcaagacaat agttcgcgcc cgtcacttcg cccatcccgc cgtaaaaagt
     5101 aagtttcatg gatatattat acggtggatt gattttgaaa acaatctatg atactcttta
     5161 acga
//
LOCUS       DAIDDT010000052         5014 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_52_length_5014_cov_6.104112, whole genome shotgun sequence.
ACCESSION   DAIDDT010000052 DAIDDT010000000
VERSION     DAIDDT010000052.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52_length_5014_cov_6.104112"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1214 a         1235 c         1398 g         1167 t
ORIGIN      
        1 gaattttcgg agcggaaagc gcgcattgca acgaaagaat atcgggatca ggatatttca
       61 aggcgaagtc cgcgctttca gaacgaaaat aaccgcaaga cccgcggatt cttcttgaaa
      121 agctattttc gagacgggtt ctaaactgaa taacaagtaa atttataacc aataaccaag
      181 ccatgcccaa ttttcatcgt tttaccatta aggcgcagga agcgctgcag aacgcgcagg
      241 agatcgcggc gaaaaaaaat cacggcgagc tgaaggcgat tcatcttttg gcggcgctta
      301 tggaagatga gcaatcgctg gtcatcccgc ttttggagcg ttccggcgta aatctcgaga
      361 aaatggacga gctgatcgat ttcgagcttg atcgcatcat gcctatcgtg ggtaataccg
      421 ccgtcagcca gctctatctt tctcaggaat tgatgcaggt tctcgatcag gcggcgaaga
      481 tcgctgcgca gcagaaagac gaatatgttt cgtgcgagca tcttttactt gcgttgcttg
      541 aggtgtcgac gacggcgaaa acgacgctgg aaaaattcgg cgtcaagcgc gatgccgcgt
      601 tccgcgtgct ggcgcagctc cggggaacgg tgcgcgtgac ggatgaaacg ccggaaagca
      661 aattccagac cttcgagaaa tacgccattg atttaagcga ggcggcgaaa gcgggcaagc
      721 tggatcccgt aatcggccgc gaagaggaat tgcggcggct gatgcaggtg ctttcgcgcc
      781 gcacgaagaa taatccgtgc ctcatcggcg agccgggcgt cggcaaaacg gcgatcgtgg
      841 agggtcttgc ccagcgcatc gcttcgggcg acgttccgga aatgttgaaa ggcaagcggg
      901 ttttgatgct tgatatgggg tcgcttatcg ccggcacgaa gttccgcggc gagttcgagg
      961 atcggttgaa agcatttata aaagaagtga aaaacgcttc cggcaatatc atccttttta
     1021 tcgacgagat ccatacgatt gtgggagccg gcgcggcgga cggggcaatg gacgcttcga
     1081 accttttgaa gccggcgctc gcgcgcggcg aactgcacgc gatcggcgcg acgaccacga
     1141 gggaatacca gaagcacata gaaaaagacc ccgcgctcga gcggcggttc cagccgatca
     1201 cggtcgaaga gccgtccata gaaaactcca ttgcgatcct gcgcgggttg aaagaaaaat
     1261 acgagatcca tcacggcatg cgcatcagcg acgaggcgat tgtcgaagcg gtcaatctgt
     1321 cggcgcggta cattaccgac cggtttctgc ccgataaggc ggtggatata atagacgagg
     1381 ccgccgcgtc tcgccggctc gattcggaaa gtttgccgaa agagatcgac gcgatccggc
     1441 acgatatcat gcggctcgag gtggaacgcc aggcgctcgc gaaagagggc gcgaaggatg
     1501 ccgcgcgcgt gaaggagatc gacaaagaac tcgcgaaatt caaagaacag aacgacgagc
     1561 tttcgagcaa gtggcatgcg gaaaagataa aattcgagac gctgcatcag ctccgccaga
     1621 aagtggacaa tttgaagcgg gaatcggaag ttgccgagcg tgaggggaac ctggagcgcg
     1681 tggcgcagat cctgtacggc gaactgccgc aggcggaaaa ggatttcact atttttgaaa
     1741 agaaaaattt cggcgcggcg gcaaaaggca agaaagcgaa gggcgtttcg gacgaccgtt
     1801 acttaaaaga agcggtggat aaggaagatg tcgcggcggt ggtttccaca tggacgggca
     1861 ttcctttgaa gcgtatgctc gaatccgata ccgaaaaact gatgcgcatt gaggaggtgc
     1921 ttcacgaacg ggtcgtcgga caggaggaag gtatcgcggc gatcgcgcac gccctgcgcc
     1981 gcgcgcgcgc gggactttcc gatatcaacc gtcccatcgg gtcgtttatg ttcctcggcc
     2041 cgacgggcgt cggcaagacc gagcttgcga gggcgctcgc ggagttcatg ttcaacgacg
     2101 aaaaggcgct tatccggatc gatatgtcgg aatatatgga acgttacgcc gtgagccggc
     2161 tggtcggttc gccgcccggc tatgtcggcc atgaagaagg cggccagctg acggaaatat
     2221 tgcggcatcg tccgtacagc gtcattcttt tcgatgagat cgaaaaagcg catcccgaag
     2281 tgtttaatct tctgctgcag gttttggaca acggccggct gaccgacagc aaagggaaaa
     2341 cggtgaattt caagaatacg atcatcatta tgacctcgaa cgcgggaagc gagcacttaa
     2401 aggcggtgtc gcgtatcgga ttcagcgcgg cgggcgcgca ggcgacgctc gaagaagggg
     2461 attatcgcga aaaagtgatg gaagcattgc gtcgcagctt ccgtcctgaa ttcctgaacc
     2521 gcatcgacga aatagtcgtt ttcaaaccgc tcggcaaaga ggacatcgag aaaatcgtgg
     2581 acatccagtt ggcgcttatc gaaaagcggc ttgccgagcg gcatatcacg cttgcgcttg
     2641 attcgacggc aaaagaatac ctgtcaaagg agggtttcag cccggagttc ggcgcccgcc
     2701 ccttgaagcg tttgatgcag aaggttatcc ttgatacatt ggccgataag atcattcgcg
     2761 gtgaattcaa agatggcgga aaagttaaga tcaatcttaa agcgggcgca ctggtcttta
     2821 atcactagag cccatctcga aaattggcgg cctatttttg agttgggctc tcgagccgtt
     2881 attttcgccg ccgcgtggct ttttctgcca tggtggcttt ttcttttcgt cgcgcttggc
     2941 tcttatttct atcatttttt ccagccatgg aaaacgtttg ccgctttttt gtgccttgtg
     3001 gcgctcacgg ccgcgcaatc gccgtctctt ttatttgcgc ttatttttgg agccgtcttc
     3061 tattacctcc ttcttatcaa ggacctgatg atcatcgacc ggcgatccgc ctatgatatc
     3121 cttgttctcg cactctcgtt tttcctgatc agggattttt tcagagggtt ttatgcgccc
     3181 ggcgcagccg cttttttctg gtcattctgg atcgctgtcg caataggatt gctcgccgac
     3241 agcatgatgc gttttttcgc ggttgataat ttttcttccg catcgctttc tgtatctgcc
     3301 agggacgctg ttcttgacgg cgatcaggag cccgtttcgg gagaaaatat ggacagcgat
     3361 gtttttaacg accatctccg tcgtcctcgc gaagtcaagg atccgcgctt ccagtattcc
     3421 tctcttcgtt attcgccgga agcaagaatt ccccggaacc gcctccggcc tgtcgcggca
     3481 tggctcatcg ccctgctttc gtggcagctg cttcttgtcg gtctttttct tccgcttgat
     3541 ttcatttatc aatccgtcat cgtttttgtc gccgccgcat ttctttatga acttatcacc
     3601 ggatatttct ttaacgatct ttcccgggaa aagacattcg taacggtcgg cgtttttttc
     3661 tcgatcatta ttcttcttct tgcttcggca acctggaaac cgtagaggtt ggctttattc
     3721 tcgtttccgc gccctgttcc ctgcgctcgg agaaaacgac aacgcgggct ggattatttt
     3781 tgagatgggg tctatactca tcagcatgtt tgaatccggc aaaacaactc ctgttctgaa
     3841 gaaatcatac gagattgcgt acgcgctttg ccggatcgcg gcgaaaattc cggaaaagta
     3901 ttttgcggat gcgcttgcga acgaaggcgt gaaactgctt ggttttgccg cggaagggga
     3961 atccggcaat gcgcaaaact cgctcgccgc cgcggagtat tttataaagc tcggtatggg
     4021 tatcggttct atcgataccg cgaacggcga attgcttctc ggcgagattg ccggactcaa
     4081 cgggatgatt gccggattat tgaatatatc caaagcaaaa cctgccgacc tttcaggaat
     4141 ttttactccg gcggaagaat ccgctctttc tgtcatcccc gcgcaggcgg aggcccacgc
     4201 ttccaatctt aatgcatctt ttaagactgg aagcataggt tatccgtctc cacggacaat
     4261 gacagagggg aacgcggacg acagaaaaac cggttccggc aataatccgg caacagaatc
     4321 cggcaacgat gattatttca taaataatcc ggcagaatcc ggcaaagaat ccggcaatcc
     4381 ggcaataaat gaacatgaat ccggcaacga cagcaactat ggcattgccg aattgattaa
     4441 atccggcaat cggcagggag cgattcttga aataatccgg caatccggca attgccgcat
     4501 caaggacgtc caggatattt tccccggatt aagtgaaaga acgctcagac atgaccttca
     4561 attgcttgcc gagcaaaact taatagagaa aataggcagc ggcggtccgg cggtgttcta
     4621 taaaagaagg ggataactat agtttctctc cttcgtcgtc ctcgacccgg tcgaggatcc
     4681 acgctttaag aaataaaaga gaagattgaa agcatagatt ccctccttcg gggagacgac
     4741 gcaataagaa gtgcgccgga ttggaagcat gggtcgtccg gtcaagccag atgatgacag
     4801 atcgagggcg gacgacggcg atatccggca gccctgtttt gccggatttt tccgctttgc
     4861 cgggtaactt tccggtacgc ccgtccgttg taatgaatga tggcattttg gaagtggaga
     4921 ggaaagggaa atgcccttct tcccaatttc agatatgtct cctatgatga aaatccttaa
     4981 ataacttcgg aagtgaggcc ggactaaaga taac
//
LOCUS       DAIDDT010000053         4945 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_53_length_4945_cov_6.356820, whole genome shotgun sequence.
ACCESSION   DAIDDT010000053 DAIDDT010000000
VERSION     DAIDDT010000053.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53_length_4945_cov_6.356820"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1261 a         1343 c         1221 g         1120 t
ORIGIN      
        1 atagtatagc atattgaaaa tattattacc gtcttcaaga tccgtttttc atggctttcg
       61 caatcatccg ctttgggtta tcccgtgccg tgaatacgga acggatgacg acgggaaaag
      121 ggatgtttgc cttctctttt tatgtacttg atatttcgac cgtatcccct atttttatat
      181 cgtctttcgc cacggtgccg gcgctcactt caagcaccgc atcaacgccg ggcggcgacg
      241 aatatatttt caatttccac agcggcgtgc cgggctgcgg cgcggcgttc tcagcgaacc
      301 cgaggacttt accctcccag gttttgcctt gcgaaccttc tgaaattttg ttctgaaaaa
      361 ttggggaggg cgaaccgccg ccgatccaga tcatatccaa aggaaagttc atgtctttca
      421 tccaaaaata cctgaccgtc gaagacccga aaagaaagac catcccgtcg ttttgcccca
      481 gacccgcgcg aaacgacagc ccgcgcgcct gctcgattgc cgtccgcgca acctccgcgg
      541 aaaaaaccgc gcttccgacc gtcacccgcg ccgcaggaag cggcggattt tcctgagaaa
      601 ggacactatg ccaaaaaccg aacaccacaa gcactaccgc tatgaacgca atgatggcta
      661 ttattatcat aattttcttt gaagtcatct tcagtaaaag gatacctttg tttcggaaaa
      721 tttgcacatc ttattacaaa tggcgtagtt caaacgccac gatgccgaac gaaaccgcga
      781 cattcagcga ttcttttccg cggccggaat gtttcggatg atcggcatcg ccgaccatgg
      841 cgccgcgcat gggaatttcc aaaattttgc cggcgcggtt caggattgac ggcggcaaac
      901 cgcggacctc gtcgccgaga accaatgcaa tttttaaatt tttacttttt ccgcctgtga
      961 cggatccgct ttttgcggaa atttttaact cgtcatacgg aaccgacttc cttgattgtt
     1021 cgacggcgaa aattttccag ccgccttttt taagcgccgc ggttaagcgc gccgtgtttt
     1081 tcaccgactc ccacgcaaca tatttttccg cgccgagcgc aaccttcgca aaatccttgc
     1141 gcaaattccc cagccggtcg tgcggcgcgg gtgtaatgcc gcacaaatat attttttccg
     1201 cgcccgcggc atcggctgtc cggaaaatgg aaccgacatt atgcacgctc ctcacattat
     1261 gaagtatgac gcaaaaaacc atactctcca ttatattaca taaggaatgt gctgaaaata
     1321 ataaaacatc ttatggataa ctatttaaaa atttaatttt cgtcatgcga aagaggaaat
     1381 ttatgctttg tcctgaaaaa ttcttaaaga atatatctcg cctccgcaag aacggccgat
     1441 aagccattat gactttttcg aatacgttta ccctccgtct tcatggtgtt tccggcggtc
     1501 attgactcgc ggcggcggtc gtggatgacg ccgcatcggg caatgcccac gtcgacatag
     1561 tcggtgcgga aacattgccg aaataatcca cggcacggac gccgatataa taagtattcg
     1621 caggatcaat tgcgcagaaa cccaaacgga tactgccgac cgattgccaa tgcaacgcat
     1681 caagcgtcgg cgatgttccg caattgatct cgtaagcgag cgacgaagaa gctgtatccg
     1741 gatcggtgga agccgaccat gaaagatcca attgtccggt cgcgggatcg acggaggtca
     1801 caatatcggt cggcgggctc ggcggcatat gcggcggaaa attcgggatg aacacccatg
     1861 aaagatatgg ataaaaagaa taggtatacg gaaccttgcc ggagccataa acgaacgcgt
     1921 tccacatcgc cgtgccggtc gccatcgtcc ccttcaaaat aacactccgg ttctggcaac
     1981 cctgataagt atagaggcga taatatttgt tcggctgaag cgcgatattc aggccgccga
     2041 ccgtaacctt cgtattggca tgtataaacg gcgcattgtc cgaaatggta aattgccgat
     2101 taagcttggt atagccggga tcgaggaatt ccccaatcca aagactcgat tcgaaaaaaa
     2161 acggatcgtc tatataacct tcaaacgtaa gcgagcggat cgtgccataa aatccagtgc
     2221 cgagatcgta ccagttgtcg tcgtaatggc tcccgtgcga atcgctgccg tcaactgcct
     2281 ggaccgtcgt ggaccaatcc ggtatggcga ccgtcgcagt cgcaaatgaa atatctcccg
     2341 caacgtcaac ggcacggata ccaaaataat aatcatggcc gtccgcagag acaggatatg
     2401 aaaacgaagt cgacgtgcca gtccataatg gcggcgtacc ggatgcaata acgtcagtaa
     2461 tagttccaga agcggtttgc ggcgagcttg tcgatgcggc atccagatcg aaaacagagt
     2521 aaacgatacc atccgatgat ccgctcgcat cctgcggcgc ggcccatata aaatcaatcg
     2581 cgagcgtgct cgaattgaaa acggcgattg catccatgcc ggcgacgtgc gacggcggcg
     2641 cgaaaacaaa aacgggggtg ggcggtaccg cagaggaagt cgcatccgat aattgagtcg
     2701 gcgatgcgga tgatgatacg acggaatcaa tactggaagt tgccgttgat gtcgaagcgg
     2761 atgcggttga cggcgatgac gtggaaattg catccgtaat cgaagaaaaa ccgtcaacta
     2821 aagtggatga tgccgtggaa gtcgtgataa ccaacgttgt cgacgatgca acggacgata
     2881 ccgcggtggt ggtcgcaacg ccgatatcgg caataataat agtagaagaa gtattgtcgg
     2941 ttgacgctgc ggatgtcgat gttgcactgt aatcactgac cgtatccgtg cccgcggaag
     3001 aagacgcatc ctcggcttgc ccaatcgttc cggatggcgc agccgatgcc aacgcagaag
     3061 aatcttccgg cgatgcggca gaaaaagacg gcggcgcgct cgttatcgtc gctgaaacgc
     3121 cgcctgtcgg aacagtttgc ccgcttgtgg cgagcgaagt cgaaccattg ccgccgccgg
     3181 acgaatcgga aatatcctgc gtagtcgatg aaacgatatc cgaggaataa ttttgcgacg
     3241 acaaaccgtt cgacgcggca ctgtccggat ctaaacttaa aaccgatgaa ttcgcggccg
     3301 gggaatttgc cggagtgaat gcgctatcga tgccgcttgg cgcagaggac aatcctgcgc
     3361 cgttattcaa actctccgcg cttgaactct ccgtagtcgc atcaaaaata ttatttccat
     3421 ccgaagatcg gcccgttgaa aacgaaaata aagaggaatt cttcgtgata atttgcgatt
     3481 gcccctttga attttcccct gaaaaatccc gagaggaagg cgccggcgcg gacgacgttc
     3541 cggaaataga agaagcgccg atgctttcgt atgatgacgt cgcgccactt attaatccgt
     3601 tatcggccgc cctattgctg tccgatccga caccgcctgc cgcgccgaac aaattaccgg
     3661 cggcttgcgc catgatcgcg ccgatatcac ccaaaaggcc cgtgtccggc gcgccgttgt
     3721 ccgaaacata ccgcgcttct tcctttatac cggtatagcc ggaaatcggc tcgaaattat
     3781 gcgccgaaaa acttccatcg gcatttttat cgtacatcgc gaaatgaaga tgcgcgccgg
     3841 gatgctccgc gcatgcagtt cccattacaa ggccggtatc gccgagtttt cccaaaacgg
     3901 cacccgtttt taccgcatcg ccgtcgttca ccgcaatgct tccctggata agatgcgcat
     3961 agcggctcac gatattgccg tcgcccgcca caagaatttc cgtgccatag cccccgttat
     4021 accctgcttc ttcgacaatc cacgcttttc ccggcgccgc cgcaagaata tttttgccgt
     4081 aagtattaca gccgtcctgc gtgaaatccg ccgcataagc gtctttatga atgtgcgtcg
     4141 gaggactgtc atacccctgc gtcacgacaa aactttgctt gttttcatag ggaagcttcg
     4201 ccgcaaacga ttgcgccact gcataccgca cgcaaaatac cggtagcgcg gcaagggcgg
     4261 ccataaaaaa catggctata aatttcttat ttcgacgaac gagcggcgct atggtcaccg
     4321 acatgtgaag acaatgcgcg aacgaagcac gctctcgacg gcgtttgaag gtcaaaaaaa
     4381 gttttttatt ttgttccatt ttcaaaatgc atgccgttaa taaattgcgt aagtgcggca
     4441 tcgcaaaccg cattgccgtc ggcctttcgt atgtccaaaa ccgaaggagc tatttcgtaa
     4501 atattcccct ccgcggcgac ataggcaagc gttgtcgcga cgccatccat ggtccccttc
     4561 cattccaaag cggcatccgc gccggacacc gaaagcccct cgctcgatat ttcggcaacc
     4621 gcaatcgtag gatcttcgct caaatgagcg gccagccaat gatccatatc cgccgcggga
     4681 atatccctaa aaagggaaac ttctattttg cactccggcg acgagcttgc ctccggacga
     4741 taatccggat agaccgcgac gccgctccct tttttatttt caagatacca gcttggcggc
     4801 aggacgaatg aataaccgcc cgttttgtcg ctgacaaaat ggctgccatc cggcagtaat
     4861 gcctgcgaaa tatcatcggc gttcaaagat gcgcccgatg gctgcgcgcc gatccgcccg
     4921 tgccgcgcca ttataaagac aacga
//
LOCUS       DAIDDT010000054         4873 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_54_length_4873_cov_5.855922, whole genome shotgun sequence.
ACCESSION   DAIDDT010000054 DAIDDT010000000
VERSION     DAIDDT010000054.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54_length_4873_cov_5.855922"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1153 a         1038 c         1286 g         1396 t
ORIGIN      
        1 ctatacatcg ctttaaagcg atgtataata aaacaatgaa atccaagaca tgtcttagca
       61 tttttgccgc cacccgcagc ggccagttgc tggtggaggt tcttgtcggc atgggcatcg
      121 gcgttttact gattatggcc ggtattgcga tgattgtgcc ggcgcttaag actaacacgc
      181 atgtaacgag cgtccagacg ggatcgactc tggcaaatga gcttttggat aacgtgcggg
      241 tgtggtcgga aggggactgg aataatgtgc tcgcgcttgc cacgggaacg gcgaaccagt
      301 attatctcgt gacggcatcc tctcctttta cggttgctac aggaaccgag acattggtaa
      361 tctccacgac gacttatacg cgttcttttt atgtgagcga tgtttatcgc gacggatcgg
      421 gtaatatcac gacgagtgga ggaacctatg acccttcgac gaaacaaata acggcggttt
      481 atggctggac gaacagcgcg accgatacgc tgtcgaccta tcttacgcgc aatcgcaata
      541 atatctatca cgctacggat tggtctggcg gcgcgagcgg taatacaggc tcgttgactg
      601 tggtggataa tcaatttgct acgtcgacga atattgattt tacttcgacg ccgggttcga
      661 ttaccatagc caatcttcag ggcgtgacaa cgagtgtatt gggcacgaat atcagtgccg
      721 ctgtgaatga ccattgggcg tggaatgata atatcggctg gattgatttt tatgatactc
      781 aaaatatcgt tgtatcactt acggggatta cgggatatgc ttcgtcatct gtgggatacg
      841 tttcgcttga ttgtggcacg gttggcgtgt gcgggacgag cgattacaat atcgcgaatg
      901 acggcgccgg aaatctttcg gggtgggcgt ggaacgatct ttatggctgg atcagttttt
      961 ggtgtggaaa tggcggtggg agttgtagtg tgccatatcg cgtgacgatt gactccagtg
     1021 gaaattttca gggttacgcg tggaacgata atatcggctg gattgatttc aattgtgata
     1081 atgtttcggg tgcgtgcgcg acatccgatt acgaggtgca aaccgcatgg actgttacat
     1141 ctaccgttgg ctttcttgat tcgcagactt tcgataccgg tgtcgtgtca ggtgcgcagc
     1201 ttaattctgt cctatggcat ggccttttgc cgacaggtac ttcggtcgcg ttccagtttg
     1261 cggtgagcaa cgcatcgagt ggtccgtgga attttatcgg ccctggcggc gctacgagca
     1321 cctattggat cgcttctgcc gtcggttcgt cactgccgct ggattataat ttatacaatg
     1381 attttcgata ttttcgttat cgcgttactt tattttctga tctcgctcat agccagtcac
     1441 cgcgcgttga tgacgttatc attaattgga gcccgtaaaa aatgagaatg aataataatg
     1501 aataatattg aagaaagata attgtaaggt ttttaatata tgcagcacgc aaataatgta
     1561 atgcaatgat tttttcaaga aagaaaggta taataatagg cgcaagaaag gggcaggcct
     1621 ttttggcgac ggttcttctt attggcggta tcgttgttgc ggcgggcatt cttcttgctg
     1681 ctctcgtttt ttcctatatt gattccggct atgggttacg ggcatcttac gtcgccgaag
     1741 cgatggcgag cgcaggagcc gaagatgcct tgttgcagct tgcgaggaat ggctcatttt
     1801 cgaatccgtc gggctatacg gttgcaatgg gagtatatac cgcgaccgtt actgtaaacc
     1861 agaatatccc gtctcaggga ctcatcacca tactttctgc tgcaaccgtt ttatttcgga
     1921 cgagagaagt aaccgcagtg gttgcggaag atccgtcgac aaatcaagta acgcttatct
     1981 catggcagga aacccaataa atctttcgcg ctgcatcaat tttatgcctc tctcaaaaca
     2041 gccagagaca aacgttaaaa aagtgttttg ccatgcgtat gcgcattatg tctttttaag
     2101 agcaaaaatt aattaagcgc tggaatgaaa aatatttttc accaagtgga agtttttttc
     2161 aataaggcgt tgtctttgtt gcacgttcag cagaacgtcg gtggtcttga cgtaagtgat
     2221 cacgtacttc ggttcgcata ttataacggg gaacaatggc gattgcaggc gttccgtctt
     2281 gcgccgggtg ttatcaagga cggcaggata aaaaacaagg acgcgttccg cattgcactg
     2341 cttgagttga aggcggaagt ttttggggcg aagtccggga aaaagaaaat aaacgtggtg
     2401 gtgtcgctca gttcggtgaa cgcatatagt caggttttca gtctgccgct tctctccggt
     2461 gacagccttg aaaaagcagt gatgcttaat ataaaaatgg cgtctccgga cgatgttgcc
     2521 aagatgtata ctgattggca ggtcgtgggg cgtgatacgg caatcggcag gtgcgatgtt
     2581 ttgagtgttt ttatcgaccg gccgctcgtc gatgagctga cggaagtgct ttcgtcagcg
     2641 ggtttcgtcg cgatggcact tgaatcaaaa gcattgatcc ttgcgcgaat gcttcgcgaa
     2701 aaaggagcga gtatcagcca tgaccaatcg tatgtggtgg tcagcgtcga tgatgtcggt
     2761 attgatttcc tcattatccg caaaggcgaa ttatattttg aatacgtgaa tctttggcgc
     2821 gatcttatgg acgcgaaagg acagattcag atggatgttt ttactgccgc cattgaacag
     2881 agtattcgcc aggttatgaa tttttatgga cagcattggc cggattccgt gagtggcgta
     2941 tttattgctg cgaccgcgct ctatgaagaa accaggagcg ccgttatcgc taacgtttcc
     3001 gtgccggtgg cgtcctacac gctttatttc aaagaccaag aaatatcgcc gaattggctg
     3061 gtcgccgtgg ggtgtggtac aaggagtatt aatttcggaa aaagaaaaaa agaaattagc
     3121 cttttgagtg tttcggccga ggaaacgtat ttgcgcgatc ggttcgtgaa ttttatggat
     3181 ttttggagag ttcttattcc ggttatcttt agtcttatta tcgttgtgct tacgggaact
     3241 gatatttttc ttgcgcgcgt acaggtcact gtggctgcgg aatcggccgc cgcgcttaaa
     3301 aacggaacca taccgggggt tgcgacggcg cttgatttgg caagccaatt taaccaatcc
     3361 gtgatgttca tacagacgat tgaaaaagcc gccgatccta aaatgccgac ggtgagcgcc
     3421 attgccgata ttgcttcttc aagcgggatt acagtgaccg gcatttcctt tccttccgga
     3481 agcggtacga ttacattcac tggcacggcg gaatcggaag accagatatt ttcttttgaa
     3541 aaaaccatgc aaggcgattc tcgatttacc gatgttgacg tgccgcttgc aggtattcag
     3601 acgccgcttg gcggaggagc gttttctttc actatgacgc tttcgagggc gaaataaaat
     3661 aaaatagagg agcaacaatg tcagatgccc tttttattcc atcttaaggc tcatccccgc
     3721 ctcccttggg gcagggatga aagtcggagc gctgctgttt ttcctcgcgg cgcggaggat
     3781 ggagaagatc ctatgacgta tcaatgcggc cacgtggccg cattgatacg tcatttttct
     3841 atacctattt tcttttcctt tattatttca gcattaattt tttcaaaaac atcgcgtagc
     3901 gtcccttcat ctttctttgt tatttttttc agcacgaaca tgcccgcttt ttttctttgc
     3961 gcttcgattt ttggacggat gccgattctg aatcgcgtaa aatttttcgt ctgcagggca
     4021 tcgatgatcg actggacgcc gcgatgtccg cccgcgtttt tcccgaaaga gattttgaaa
     4081 tcgccgagcg gcaggtcgct ctcgtcatgg atgaccgcga tcgcttccgg ttttacgccg
     4141 aattcgcgcg cggcggtccg gatcgccctg cctgagtcgt tcataaaaac gaggggtttt
     4201 atcaatacaa cgccgttctc ttccgcatat tcaaaaagtt ttttgcgttt gaatttcggc
     4261 ggagcttctt gatcgctccg tttttttgcg aagaacgaaa gcgccatagc gccggcatta
     4321 tggtaggtat tttcgaattc cttgccggga ttgccgagtc ccgtaatcag cttgtatgtg
     4381 gctgtccgta ttttatccgg cgaaactccg gcggatttat cctcggaaat tttgtcaggc
     4441 aaagttttgg agggtttgct ttttttttct tcaggcatat aattacgata attatcgcat
     4501 tttcagcagg gttttgccag ccgataaaat gtatttatca tgtcgttcct gcagaggcga
     4561 tcccccaaaa acttttcgga gaaaaatttt ggcgggcggg gatcgcgttt tgaaaaatca
     4621 agatgaaaac ctatcctgcc tcccatcgaa agagcggagg gaaccggaag cgtggattat
     4681 ccggccaagc cggatgatga caggaaaaat gggaagcgca ggacaacgcc aagaggggac
     4741 aataaaatat aaataaataa taatgagaaa attcttagct tcttttcttg aggtcgccga
     4801 ggtcgttgtc attgcggtcg cggcggtttt catcgtgcgg acgtttatcg tccagccgtt
     4861 cctcgtgagc ggc
//
LOCUS       DAIDDT010000055         4837 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_55_length_4837_cov_5.707983, whole genome shotgun sequence.
ACCESSION   DAIDDT010000055 DAIDDT010000000
VERSION     DAIDDT010000055.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55_length_4837_cov_5.707983"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1188 a         1106 c         1307 g         1236 t
ORIGIN      
        1 attcagaact taccctgttt ttatcaatgg tgttttcgaa aaataaaaag aaaacagaga
       61 ggggcactgg agcaatcgtc ttaatagatg gagtatagca tgagccatgt ttaaaagtca
      121 aagatttttc gatgattatt cggatttttc cattcagtcg tctttgtcct cgatctccgc
      181 atcaaagtgc ggggtaaact tcgccgtggg caagctccgg ctcgttcgtc caaatttttc
      241 gggggaaaaa tttggacggg cgggaattca tgctttcaat tttccccctt aaaagcatga
      301 atcctcgcct ccgcgaggac gacaggcgat tacggaatat tatcacttta aaacccgtgt
      361 cgtgtcgagg taccacgcgc tgacggtgcg aaaaagttcg gaggggtcgg caaggggtcc
      421 ggtcgtaata ccgtggacgc ttttgtctgt tatataaaga taatcgggcg aataaagaaa
      481 gacggcggga ttatctcccg tgatgatatt ctgtaaatct gcgaatgctg cggtgcggga
      541 ggcttcgtcg gaactttcac ggatcgactt aaggagagca tccgcttttg ggttgtcgta
      601 catcgagaaa ttaagccccg gggcggcggc ttgcgacgaa ttccaaaacg aataaagatc
      661 cgagcttggg ccgaggacat ttccgaaaag cagggcgtca taggaacggt tcggtatgtc
      721 ggcgtttgca atgttgtccg gagagtcggg agcgatggtc acgcgcacgc cgatattctg
      781 ccatgcggtc tgtatcaaag aggcggtttt cataagaaaa tctatctggg ggacggtaat
      841 ggtgattgcg agaggaatcg tgttttttcc gacggtcttg gaacgaaaac catcgccgcc
      901 cattttccag ccggcgttat ccagtgtcgt ggatgcgaaa tcaggcgatg tggtcgccgc
      961 gatgggaatg gtatatgccg catcgggggg aatgggtccg taatcggatt tgccgaatcc
     1021 attcaagacg gcgcttacga tgcttttgcg gtctatcgcg gaagaaagcg cttcgcgcac
     1081 ggccggatcc gcaagcgcgg gattttccgt ctgatttaaa aataccgcat aatagcccgg
     1141 cgtccggaac gtgaaaagat catagggtct tgtgatattt gcgatatcat ccggcaatgc
     1201 gccgccgaag ccgttgattt gcgcggtgtt aaaactttta ataagcgaat ccttgtccgc
     1261 cgaaaactgg aaatcgaagt tttgaataag cggtttcgtt ccgaaataac cgtcccatgc
     1321 ttcgaggtga taggcggtga taaagccgtc agggcgtttt tcgtatgaaa tgaacttata
     1381 aggcccgctt ccgataggtt tcaggttgtt gtccgacagc cgccagtttg ccggagggat
     1441 agcggcgaaa atatgcttgg ggaggatata aagatttgac aaatcgtcgc caaagaacgc
     1501 atagggcttg tcaagcgtga accggacttc cagttcgctc acgcggctta ccgaaattcc
     1561 tgcccagtta tcgaaaagag gggaatttgt gtctttgttc tggatttctt caaccgtgaa
     1621 taccacgtca tcgctcgtta attgcgcacc gtcctgccag tggagatttt ccttgaggcg
     1681 caccgtccac gtcttcgtgt cggatgaaac ttttatcgta tcggcgacgt cattgatgtt
     1741 gctgtaaatc attttgacga ggtcgcggtc ggtttcgctt acggccgtta cgggatttac
     1801 gtattccggc tgtcccgcga tgccatccgt atattcgccg ccggtcgacg gtaccgtctt
     1861 ggtggttttt acaatcacaa ttcccgcgat gatgataacg cttattgcga ctactcccgc
     1921 ggcggcaaaa aatgaaacgc gttccttcgc cgtcagcgca ttaaatattt ttttaaagaa
     1981 agacatcttt tatcatcctg gaaaatgcga gtcttcgagg gtttatccgg catccaggtt
     2041 ttgaatttta attaaaaaga attaatgctg gatcctgagt agccggggcg gattccggat
     2101 cattcggttc cgggggtctt gtgctcgacg agacggcagc ataacgggag cagagaacgg
     2161 ataatattct tacgggacaa ccagttctaa tgcattccag cggcgagtcc atagagagca
     2221 accgccacga agagcgtaac gagaacgatg gtcgcgtaga atatgatctt ttcggcgccg
     2281 cggcgcgcct gataaaatcc gatgccgtcg ccgcccaaaa ggccggacat gccagaagaa
     2341 cgctcttgaa gaaggatgag cgcgatgatg gcgacggaaa gtataatttg gatggcggca
     2401 atcatgagaa gaagttaaaa attaaaagca gctaaagatt aaaaaattat aaagcgaaac
     2461 atccataact tttcattatt cgcttttaat ccttcactta tttattccga ggtggattac
     2521 gaaattaatg acgccgagaa gcaatgtggc atagatgagc gccggaacgc tctctatgct
     2581 aatggttccg cgcagaatgt caagattatt actgagaaac tggagcataa ggagaatggc
     2641 cgcgtttacg acaatgacgc cgaggccgag ggtaagaatg atgatcggtc cgaaaataag
     2701 cgtaagaatc ggcttcagaa cggcgttcag gattgcgagc aggagcgcga taagggcaag
     2761 ctgagaaatg gcggcaacga gaacaaaccc cggaatccat aagcttgcga gccaaagtcc
     2821 caaggcattt ccggcgacaa tgataattat ttttccgagc caatgcatgc ctgaagtata
     2881 gcatataaca cacgcccgcg cacaacaacg gatgtccgca aaatgctttt atcaaaacga
     2941 tcgttttgat aaaagcattt ttatcttttc tgcggaaatc cgcgagcgaa aaattggaac
     3001 atttattcaa tcgggacgga tctttccgta ttaagcgtcc gttatcacac gatctcttcc
     3061 aataaactat ttccggtcat tgcatcgggt tttttaatcc ccataatttc aaggatagtg
     3121 ggagcgacat ccgcaagcgt gccggcggtt tccgatgcca gccggccgta gttcgcaaat
     3181 ttctttcctt tatgttcttc ggtgacgaga taaaacggta cggggttggc atcgtgctgg
     3241 ctttccggaa ggccggtcat ccggtttatc atttcttcta tattaccgtg atcggatgtg
     3301 ataatgagca gtgtttcggg gttgatggcg gccttgagta cgcgaccgat ttcgcggtcg
     3361 attacgcgca ccgcctcgac ggccgcgttg tagttcgcgg tgtgagcgat ggtatcgggg
     3421 ttagcgtaat tgaccaatat gaaatcgaat ccgcggcttt gtatcgcttc aataaggcga
     3481 tcggtgatcg ccgccgccat catttcaggg tgttcctcgt aatgaagcga cgggagcgac
     3541 gggacgagcg tccggtattc gcctttataa ggtgcctcgt gcaggccgtt gaagaaataa
     3601 gtgacgtggg catatttata agtctcggcg atgcgaagct gtgtgagtcc cagatcgctt
     3661 atcacttttc cgagcggcgc cgttaccata tcggcgggat aggcgacggg aaccgaaaaa
     3721 cggtcgtcgt aatggctcat ggttgcgaca taaagattct ggaagttcag gacggggaat
     3781 ttgtcgaaat ctttcagtat aaaagaagac gcgatctgcc ggatgctgtc ttctcggtaa
     3841 ttgaagaaaa taaccgcatc gccatccgct atttttttgt cgtcggcgaa gcgcatcggc
     3901 ggaagatatt cctcgctgtt gccgtcgcgg tatgtcgcct gagcgatcgc ggcggggtcg
     3961 tcaactattt tgccgagctg tcccgtcatg gtctgatatg ccgtttgcgt gagccgccag
     4021 ttgccgtcgc ggtccatacc gtaatagcgg ccgaccacgc tcgcaagata ttcgcgcgga
     4081 atttctttca ggaatttctc gaaggtgcgc ggcggactgt ctttcccgtc gccgaaaaga
     4141 tgaaggttga ttttttccat tttttcgccg cgcgccattt tgatgagcgc ctgaagatgg
     4201 ccaagcgatg catgcacatt cgccttcgtg agaagcccca cgaagttcac cgcgccgcca
     4261 tgcgcgcgcg catgagtaaa cgcgtctaaa aatgccttat tttcaaaaaa gctgccgtcc
     4321 tcgatcgcca tcgtgatctt cggataatat tgatagagca cttttcccgc gccgatggtc
     4381 agatggccga cctcgctgtt gcctgtttcg ccccacggca gtccgaccgc gatacccgat
     4441 gcctgcaggc ttgtcatggg atagttttcc tcgagccatt tgaacgtcgc cggtttcacg
     4501 ccatagatcg gattcgattc atcgttgctg ccgacgcccc agccgtcgag gataataagt
     4561 acggtccgtt tttttgccat tatttcattg tagcgggaaa aaggatattc cgcactgcgg
     4621 ggcttgcgta atcttatttt tatgttattc taaaaacagc tttttaacag tttttaattt
     4681 tatagagata ttaagaaagg aggggtatga caagcaagcg gcggcttcgc aaaaaggaat
     4741 gcggggagaa aattaaattt gacagtaagg cgggggcggc cgaagccgcc ttactctatc
     4801 ggcgcagaca ctcggatccg aatgtccatg aatacgg
//
LOCUS       DAIDDT010000056         4521 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_56_length_4521_cov_5.691494, whole genome shotgun sequence.
ACCESSION   DAIDDT010000056 DAIDDT010000000
VERSION     DAIDDT010000056.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56_length_4521_cov_5.691494"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          940 a         1134 c         1150 g         1297 t
ORIGIN      
        1 aaattaaaaa gaatactcga attgcaagta aatctcttta tcggatgtat ctggctggta
       61 accaaatagt ccgttgccga ggccgatgtc gttagatttc ttccattttt tcaattcata
      121 ggaaagactg aattttattt ttcccgaatg aaagccgatt tccgcaaagg gcgtaatcac
      181 accatttggc cttgtcgaaa attttgtcaa ctggccatcg ttttgcatca agataatcca
      241 gttttctgcg tagaccggga aaaacaatcc ggtctttatg aaaaacggac ctttccctgc
      301 tttaaacccg acttttccat agccggtaag ccagatttcg ccagagattg accgtttcca
      361 cgcggaaact ccagcggaaa caaacggcga aacataaaac ttattgggaa tttttatctt
      421 ataccccgcc gttccttcca gtgttgtccc tatccttagg gtatccatat cacaaggagc
      481 gtcatttgat atgtataccc ctttataacg gagcaatccg ccaaaaccct ccgctttgat
      541 ctcgccgaaa aaattctttt tgggatgaaa cgaaaacttc gcgcccaggg tatagatttg
      601 cccgctttcc tggtcataag aattcccatt cagtgtttca ttccaaaaca tccatccatc
      661 ttttgcataa acttttacgc ccgcggcatg tgtggacgaa gtgcacacag cagaaatcaa
      721 gataacaact ataactgaag ctaacttttt caccattctc cctccctttt aaagttattt
      781 tccagattga ggcatcatat atcaaccctt tttaaattgt taagtactct atacaaagta
      841 ctctttaaat tatattttgt caagctccaa tttttttgcg ttatcgcaga tcatttttgc
      901 ctggccgtcg cggacggata tcctgccctg gatgatgacg atattgtttt caatccacac
      961 gggaacggtc ttttcgagga cgctcgtgaa gacgacgatc tcaagcgatt tctgggcggc
     1021 gtcttcgatg gtcgcgaaaa gcataggttg tccgcttttc gtgaaaattt tccgcacttt
     1081 tgaaacgagt cccgccgcgc gtacgacgag gttttcgttg gtgacggccg caagttccgc
     1141 aagcggtttc gcgttatatt tttttatttt ttccgcgtag ccggtcagcg ggtgttccga
     1201 gagataaaaa ccgatgagct ctttttccca tgcgagcctt tcttcgttcg tcgcgggagc
     1261 cgccggcttg agcttgagcg ccgcctgcgg gatgccggcg gcgccgaaaa gggaattcgc
     1321 cgcgttctcg gtgttccgtt tcgcggcgtt cacgaacttc aggatgtcgt cgatgttttc
     1381 gagcgcctgc ttgcgttcga tgccgagcgc gtcgaacgcg ccgcttttca ccaggctttc
     1441 gatggatttt ttattgaggt ccttatgctt gatccgcatg atgaagtcct cgaaattttt
     1501 gaacgcgccg ttgcgcgagc gttcttcgat cacggctttc gtgatttcgg aaccgacatt
     1561 ctttatcgcc aaaagcccga agcggatatg cgcgccttcc ggcgtgaaat tcacgaagct
     1621 gtggttcacg tcgggtccca ggatgctgat cttcgcctgc cgcgcttcct gcacgaggag
     1681 tgcgatgcgt tcaatatcgt ccatttccgc gtttaaaagt cccgccatga attcttcggg
     1741 atagttcgct ttgaggtagg cggtttcata gccgatcatg gcatagcacg ccgcgtggct
     1801 cttattgaaa ccgtagcgcg cgaacggcgg gaaaagttcc cagatcgccg ccgcggtttt
     1861 cggcgggatg ccgtttttga tcatgccgct cgtgagtttt tctttctgtt catcgagcag
     1921 tgattttatt tttttcccga tcgctttgcg caaggtgtcc gcttcgggga gcgtgtagcc
     1981 cgcaaggtcg cgggcgatct gcatcatctg ttcctgatag acgccgatgc cgtacgtatt
     2041 tttcaaaatc ggttcgagtt tcggatgcag ataagttatt ttttcctggc catgtttgcg
     2101 cttgatgaac gacgggataa gttccatcgg tcccgggcgg aacaatgcga cgagcgcgat
     2161 aagatcttca agttcggtcg gcttgatgtc tttcatatag cgccgcatgc cgctcgattc
     2221 gaactggaac acgccggtgg tgtcgccttc gcggagcagc gcgaacgttt ttttatcgtc
     2281 aagcgggatt ttcgagatgt cgatcgtgac gtcgtgcagt tcttttatga ggcggagcgt
     2341 gttttcgatg atggtgagat ttttgaggcc gagaaaatcg attttcagaa gcccgagatc
     2401 ttcgacggcg tgcatttcga attgcgtgat gatgatttct ttgtcctgcg gggcgtattg
     2461 caaagggacg cgttccatga gcggttcttt cgcgatcacg gtgccgcacg cgtgcacgga
     2521 cgcatggcgc gcgacgcctt caagatgccg cgcggtgtct atcaattttt tcgcttcggc
     2581 gttcgtatcg tagagcgcct tgagttcgac gaccttttcg aggcattcgt cgagccagcc
     2641 ctctttcatg ccctgtgtcg ggttcacggg gatgagtttt gcgacctggt cgcagaagcc
     2701 gtaggcatag cccatcgcgc ggccggcgtc gcggattgcc gcgcgcgccg ccatggttcc
     2761 gaaggtgatg atgcgcgcca catggtcttc gccgtatttt tcgcgcgcgt acgccagtac
     2821 ttcgtcgcgg cgggtatcgg caaaatcgat atcgatgtcg ggcatctgga tacgctcggg
     2881 attgagaaaa cgttcgaaaa gcaggttata ggtgaggggg ttgatgtcgg tgatgtcgag
     2941 aatgtacgat accaggcttc ccgccgccga accgcggccg gggcctacgg cgatgccgtg
     3001 gatcttcgcc caatggatga gatcctggac gatgagaaaa taatcggcga agccggtttt
     3061 ttctataaca ccgatctcgt attccagccg gtctttcatt tcctgcgtct gcgcttcggg
     3121 cgggattttt ttcggcagcc gttctacgac aagttctctg agataatcgt tcgccgtttt
     3181 tccgtccggc ttcgggaatt cgggaagcag tactttgtcg agctcgagtt cgacattgca
     3241 gcgcgccgcg atcttcgacg tgttttcgac ggcgcccggg atatgcgcga acttttccgt
     3301 catttcctcg ggagtgtcca gtgaaaaatc gtcttctttc atcgagagcc ggtcatcgtc
     3361 cgaaagctgg ttgccggtct gaatcgcaag aaggacatcg tggtatgccg cgtcttcctt
     3421 ctttaaatag tggagatcct gcgtcgcgac aagcggaatg ccggtctctt ccgaaagttt
     3481 tatgagcgat gattctattt tttcggaagc gggaacgtgc acgtgtttct ggatttcgag
     3541 aaaataatta tctttgccga atatggactg gtattcttcg gtaatgcgct tcgcttcctc
     3601 gaaccgttcc gcaagcagcg cctgtccgat ctcgccgccg agacacgccg aaagcgcgat
     3661 cagtccctcg tggtgttcgc ggagaaggtc cttatccacg cgcggacggt aataaaatcc
     3721 ttcgagatgc gccttcgacg aaagtttcac gagatttttc cagcccattt cgttctcgca
     3781 gaggaggatg aggtggtaat agcgctcgct ctgttctttt gaaaaacggt cacgtggcgc
     3841 aacgtacgct tccatgccga ggatcggttt gatacccgcc ttttttgccg ctttataaaa
     3901 ttcaatcgcg ccgtagagca cgccgtgatc ggtcaaagca atcgagtcca tcccgagttc
     3961 ttttgtccgc gcgacgagct cgggcacttt tgcaagcccg tcgagcaggg aataatggga
     4021 atggacgtga agatgggtga atttcggcat aaagaatatt tttgaattta tataggattt
     4081 acgcacgtgc gccgagtacg aaatattttt tctttgtcat cgtccggctc cctctattct
     4141 gttatcgtcc tctctctgtc atcgtccgtc ttcacggacg atgacagagg agaaattttt
     4201 ttatgatttt tcttcaaatg cgtaagtctt attttattat tgctgaccgc cgccatttgt
     4261 tcgaattcga tcggggcgaa ttccgagcca cctcccggat tcggaccggg gaccttcgct
     4321 ttacaaaagc gttgctctac caactgagct aaggtggcgc gtttctatct tacctcatca
     4381 tctccttttt ctgaaatctt tttcagatat atagacgatc ctccattcat attgacctca
     4441 cctcgtcctc ggaccatatc tatcactttc tttgatggtg gatgagcgtg agccttccta
     4501 tgagcccata tatacagttg g
//
LOCUS       DAIDDT010000057         4401 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_57_length_4401_cov_5.373959, whole genome shotgun sequence.
ACCESSION   DAIDDT010000057 DAIDDT010000000
VERSION     DAIDDT010000057.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57_length_4401_cov_5.373959"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1001 a         1097 c         1284 g         1019 t
ORIGIN      
        1 ataaattaca atgattaaaa aacaattttc aacggaggtt gcggggaaaa cattgacatt
       61 cgagatttcg gcgcttgcgg agcaggcgaa cgcggccatc gtcgcgaaat acggcgaaac
      121 ggtgatcctg gcgacggcgg ttatgtcgcg gagagacagc gatatcgact atcttccgct
      181 caaggtggat tacgaggaaa aattctatgc ggcgggaaaa ataatcggca gccgttttat
      241 ccgccgtgaa ggccgttctt cggaagaggc gattcttgcc ggacgtcttg tcgaccggac
      301 gatcaggccg cttttcaata atcgcatgcg gcgcgaggtt caggtggttg tgacggtgct
      361 tcagattgac gaagaaaacg atccggagtt cgtggggctg ctcggcgttt ccgccgcgct
      421 tcttatttcc gatataccgt ggaatggtcc ggtcgccggc gtccgcgtgg cgcagttcga
      481 ggacggcata aaaataaatc ccgataattc ttacgttatt gagcatccgc cggttttcga
      541 tatgttcgtt tccggcgcga agaaccgcat taatatgctc gagctcggcg gtttggatgc
      601 gaaagaaagc gatgtcgtcg cgggatttga ggcggcgcaa aaggaaataa ataaattgat
      661 cgattttcag gaaagcgtcc ggaaggagat cggaaagccg aaagaagacg ttgcgctttc
      721 ggagccggat gaggcattgc gtgcggcgat cacggcgttt attgacggta aactcgaagc
      781 ggccgtgtat cacccgagca agatggagca gcagagcgag atcggaaaat tgaagcatga
      841 actttttatg cacctcaagg agttgtttgc ggaaaaaaag gatatgaatt ggaaagccgc
      901 cgattttctt tttgaagacg cgatcaataa cctggtgcat aagaatgttt tggaaaaaga
      961 gaagcgcccc gacggacgga aactcgacga attgcgtccg ctttcgggcg aggtgggttt
     1021 gttttcgcgg acgcacggct cggcgttgtt cacgcgcggc aatacgcagg cgctcgccgt
     1081 aacgacgctc gcggcgcccg gcgcggaaca gatgatcgag acgatggaga cgagtgaaaa
     1141 gcggcggttt atgctgcact ataatttccc gccattctcg gtcggcgagg taggattttt
     1201 ccgcggaccc ggccgccgcg aaataggcca cggtaatctt gcgcggaaat cgcttgagcg
     1261 gcttcttccg accaaagagg aattcccgta cacgatccgc gttgtttcgg agatcatgtc
     1321 atcgaacgga tcgtcatcga tggcgaccgt ctgcgcgtcg gcgctttcca tgatggatgc
     1381 cggcgtgccg ctcaagaaac cggcggcggg tatcgcgatg gggcttatga tgtccgatgt
     1441 cgataatttc aaagtgatga ccgacattca gggaccggaa gatcatcacg gcgatatgga
     1501 tttcaaaatt gccggaacga aagacggcat cacgggaatg cagatggacg tcaaagtgga
     1561 cggattgacg atcgagattc tccaaaagac gatggagcag gcaaaaaaag cgcgccttga
     1621 aattcttgaa acgataacaa gcacgcttcc ggccgggcgt gaaaaacttt ctcccttcgt
     1681 gccgaccatc cgccagttga agataagccc gtcgaagatc ggtgcggtga tcggccccgg
     1741 cggaaaaacg atcaacggac tgatagagaa atacgagctt gcgggcatca acgtcgatga
     1801 tgacggcggc gtttttgttt cgggtaccga cctcgcgaaa gtcgaggcgg cggtcgccgt
     1861 gatccgcggc atcacgagag aattcaaagt cggcgagatt gtcgaagggc cgatcataaa
     1921 aatcatggat ttcggcgcga ttatggatct tggtggcggc aaagatggta tgattcacgt
     1981 ttcggaactc aagaacggtt atgtgaaaaa tgtcgaggaa gtggtaaagt tcggcgatgt
     2041 cgtccgcgca aagatcatcc gtgccgacga tgacggccgg atagggcttt ccataaagca
     2101 gttggaataa taaaaagcga ttagcgattg gctgttagcg gttagcaagc ataagcgcaa
     2161 ttcctttttg cgtcgtccac gtcccgtatc gtggtacggg aatcaagggc cgacgatgac
     2221 cgaagagaag tgtcttgagt atgtataaaa agctgccaac cggcggcttt ttatgatatc
     2281 attgcattta tggatgaact cggcatcgat aatacagcgg aaagcgatag cggcgcttct
     2341 tcgaaatcgt tcgcgaatat tcccgcgccg acggcggaag tgaaagtgcg tacgatgcgg
     2401 tcagatctgg catctcttac ggcaaccggc ggcggtttac cgcggtttaa taaagtgaac
     2461 gttgaagggt tgtcgattgc gagggctgcc gctcctgttg cggaagcgaa gccgcaaaaa
     2521 agaagcaatc ttatccttgt gattgcgatc gtcgtcgttg catgcgtcgc gctcgcggtc
     2581 ggtggattgc ttttatataa tactttctca aatcagggat cgacggcggg gcagggtgcg
     2641 gcgtcgaaca acgcccagcc ggtttcgccg ccgacatcca gtcccgtgca ggcatatgtt
     2701 ccgccggcag cggcgacgac atcggcaccc gtagttttta ctcacgtgtc gttcttcaaa
     2761 aagcccgccg accagacgat aacgctttcg ctttcctccg gcggggcggc gcagacggcg
     2821 agcgaactcc agacgtatga tcagcaggtg cttggcgttt tggcgtcgac gacggccggt
     2881 gcgcaattga tcgagatcga tgtgaaagga ggcgacggca acgccttgag cattgaagag
     2941 cttctttcgc aggccgatgc ggaaattatt gatccgaatt ttctttcctc tcatttcaat
     3001 cccgatgcca cttttttcgc ctaccgcgat aagaacggtt tctggccggg atatgtcatc
     3061 gctttgaata cgggggaaaa caaaacatcg ctccagagcg gcgtccaggc catcgaatcg
     3121 tccgcgaaga tcggcaatat ttttcttgcg ggagccggca ccgcgtcgcc ctccggtttc
     3181 ggcgatgcca tgatctcggg cgtccaggtg aggattttga attttaccgg cgttgcgcct
     3241 gccaatggcg gacagaccgt gtccgcgtct ttcgtatacg gctggttccg gaactatctc
     3301 atcataagcg cctcgcgcga gggctttgcg caggcagtgg cacgactgta ataaataaaa
     3361 aactatcaaa ttgacttttc cgtcgtcccc gtcccgtatc gtggtacggg aatcaagggc
     3421 ggacgatggc aaaaggaagg atgacgagaa acgagtttat ttgaaataaa ccctacgctt
     3481 cttcttttgt attttttacc ggtcgtatat cggggttttt cattgcttca ataaccccct
     3541 ttttaatttc ttccagcagt tttttatctt ctttgagctt cgcgcgcgcg ttttcgaatc
     3601 cgacgccgag ccgctcgccg ttgaagctga agctcgcgcc tgcttttttg actacgccgc
     3661 gggtgaatgc cgcgttcagg acgtcgcctt cataggagat gccttcgccg aaaaggatgt
     3721 cgaattccgc aagcttgaaa ggcggcgcta ctttattttt cgctattttc gctttcacgc
     3781 gattgcccac gatatcttcg cctttcttga cctgggcaat gcggcggata tcgatgcgca
     3841 ccgatgcggc gaatttcagg gcgaggccgc ccggtgtcgt ttcaggattg ccgaacataa
     3901 cgccgatctt catgcggagc tggttgatga aaataataag cgtctggctt tgattcgcga
     3961 tcgcggtcag tttccgcagc gcctgcgcca tcattcttgc ctgccgtccc acatgctggg
     4021 ctcccatttc gccttcaagt tccgcgcgcg gtgtgagcgc cgccacggaa tcaacgacga
     4081 cgacggagat catcccggag cgcacgagac tttccaggat attaagcgcg tcttcgccgc
     4141 aatcgggctg ggaaatcaaa agttcatcca cgttcacgcc gagttttttc gcgtattcgg
     4201 gatccatcgc gtgttcggcg tcgatgaatg ccgcccgtcc gccctgtttt tgcgcctgag
     4261 cgacgacgtt cagcgcgagc gtggtttttc cgcttgattc gggaccgtag acctcgacga
     4321 tccttccgcg gggaagcccg cccacgccga gcgcgaggtc gagcgagaac gatcccgtgg
     4381 ggatcacggc tacgtccaca t
//
LOCUS       DAIDDT010000058         4397 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_58_length_4397_cov_5.557870, whole genome shotgun sequence.
ACCESSION   DAIDDT010000058 DAIDDT010000000
VERSION     DAIDDT010000058.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58_length_4397_cov_5.557870"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1085 a         1064 c         1325 g          923 t
ORIGIN      
        1 gttgttaatg agctatggga tatgtcaaag tatttttttc attataacca gacaatcctg
       61 tcgccgcaat cgtcgcggcc gtaacccctc gctatttata aaaccagcgc cgattccatc
      121 gaagcacttt cccctccggt cgtcccccga aggaggggaa aatgttcccg gtcttaaatt
      181 cttaaagcat ggatcctcgc ctccgcgagg acgacggaaa aagcgcggat cctcgaccgg
      241 agcctgccct cgacccgatc gggggtcggg gacgacggaa aagaggggcg cgaggggtta
      301 cccgcggtcg ccaagcggga aacgcaaaat tgaatatttt tcgcatgttt gctatactgt
      361 aaccatgaaa ataaccatta aaaaaataac ggattcaacg ccggcgttcg aagaatatgt
      421 ggaagaaaaa ttgctgccgc ttgcgaaact gataaagcct tttgagaaaa acggcgaaac
      481 ggcggtcgtg cttgaaattt tccgcacgac gcgccatcac cggaaaggcg acgaggtgtt
      541 tacggcggtg gccgaactgc gtttgccgaa gaagatgatc cgtgcggaag aatatgccgg
      601 cgctatgcgc ctggcgatcg atcgcgtgcg ggatacgctc cggcttgaga tcgggaaata
      661 taagacgcag ttcgttgagc ttgataagaa aaaattaaca aaggaataat gcttgaaagt
      721 ttcggaaaat ttttcaagaa gcgttcgctt gaatcgcttc aggttttcgt ccgcgcgatc
      781 aatgcgctgg aagcggagat tacgccgttg agcgatgatg agcttcttgc ggaaagcgaa
      841 aaattaaaac ggtccgttgc ggacggcgcg ccgcttgacg accttttgcc gcgcgcattc
      901 gcgcttacgc gcgagacggc gaagcgggcg ctcggccagc ggccgttcga cgtccagctg
      961 ctcggcggca tcgcgcttca taaaggatcg gttgcggaaa tgatgacggg cgaagggaaa
     1021 acgcttgcgg cagtcgcgcc ggcgtatttg aatgccttaa ccggaaaagg cgtgcatgtc
     1081 gttacggtga acgaatatct cgcccgccgc gacgcggtat ggatggggca ggtctaccgc
     1141 gcgctggggc tttccgtcgc atgtcttgtg ccgaacgccg cgtttttgta cgatcccgaa
     1201 tggaagatcc ccgaagacgg catcgcgctt gcggacaagg aacgcgatgc gacaggaagc
     1261 tttcttgtcc agcaggaatt cctccggccc gtttcgcgcc gcgaggcgta tgccgcggat
     1321 gtcacgtacg gcacgaacca tgagttcgga ttcgattatc tccgcgataa tctcgtgtat
     1381 cgcctggaag acgaggcgca gcgcgggcat catttcgcga tcatcgacga ggtggacagc
     1441 atattgatag acgaggcgcg tacgccgctc atcatcgccg cgcccgacgc gcaatcaagc
     1501 gatttttaca aaacgttttc gcgtatcgct gcgaatctcg tgaaggacga agattacgag
     1561 gttgacgaga aacggaaaac ggcgaccgtt acggatgccg gcgtcgcaaa agtggaaaag
     1621 atgatcggcg ttcagaatat ttacgcgccg gaaaatatcc gcctcgtcca ttacctcgag
     1681 gaaagcatca aagcgcaggg gctttttttt cgcgaccgcg attatgtcgt caagaacggc
     1741 gaagtcatca tcgtggacga gttcacgggg cggatgctgc agggccgccg ttatcaggcc
     1801 ggtttgcatc aggcgattga agcgaaggag ggcgtactgg tgaaagagga atcaaagacg
     1861 tacgcaaaaa tatccgtcca gaattatttc cgcatgtacg ggaagatcgg cggcatgacg
     1921 ggcaccgcgc agacatcggc ggaagaattc tataaagtct atgacctcga agtggaaagc
     1981 attccgccga atcgtgtttt ggcgcgcaag gacgagaatg atctcattta ccggaatttc
     2041 aaagcgaaag tcgatgcggt cgtccgcgag gtaaaagcgc ggcagaaaaa aggccagccg
     2101 gtgcttctgg gaacgacttc catcgcgaag aacgagatct tttcggcggc gctcgcgaat
     2161 gcgggcgtgg cgcacgaggt tttgaatgcg aagaacaacg aacgggaagg cgccatcatc
     2221 gcacaggcgg gacggtccgg cgccgtgacg gtcgcgacga acgtcgccgg ccgcggcgtc
     2281 gatatcattc tcggcggcaa tccgccggag ccggaagaag cggcgaaggt gcgcgcgctc
     2341 ggcggtttgc atattctggg caccgaacgc cacgaagcgc gtcgtatcga caaccagctg
     2401 cgcggccgcg cggggcggca gggcgatccc ggctcatcgc aattctttct ttcgttggac
     2461 gacgatcttt tgcggatttt cggcggcgac aaactgaagg gactgatgga acggttcgat
     2521 ctgccggaag aagagccgat tacgttcggt ttcgtttcaa aggcggtcga gcaggcgcag
     2581 aaaaaagtgg aaggcgcgaa tttcgatttg cggaaacatt tgcttgagta tgacgatgtt
     2641 ttgaacaaac aacgcggcgc ggtttatagg cgccgccgcg agatattgag cattatgaat
     2701 aaagacgatg ccgcggcgtt cattacggcc gcgaccacgg gttattggga cctgatgtac
     2761 ggccaggcgt catccgaaaa agcggaagag gtgaagcggc tgttcgacga atccggtctc
     2821 gtaaaaagcg ccggcgatgc cgctgccgcg gttgattttt ctgacagcac ggcgtgccgg
     2881 aaaataatag aagaaaaaag cgccgcagcg gcgcaaagtc ccatggcgaa aaaccagctt
     2941 gtaagcgttt tggacatgct ttggatgaac aaccttgagg accttgaagc gctggcggaa
     3001 tccgtcgggc ttcgcgcgta cggccagcgc gatccgctcg tggaataccg ccatgaagcg
     3061 agcaagtttt ataaagactt ctggaataac ttcaacgcga tcgtgtttat gaatatcttc
     3121 aagctggcgg ccggcgatca gcaaccggcg gcttccgtaa atccacagca gcagtcagct
     3181 tccggcaatc aatttaccgg cgtcggccgg aacgatccgt gtccgtgcgg cagcggaagg
     3241 aaatataaga agtgcggtct tgccggaacc gaagaacatc gggccaatat ggcgcgaaaa
     3301 tcaggcggaa agcacgaggt gacgggcgga tagatatttt taaaacaaga aaattttgtc
     3361 gagaatccgt ttcaaaagca gttttccgtt ccgaaaattc cctgcgtaac ccgccggcct
     3421 gttttttgag acagggttct catttacgcg gcaaagcttg aaatataggg caaggcagat
     3481 tttttcaggc gtctttaaaa tgattgatcg ttatgccatc ggttgtatcc gcgtgaaaat
     3541 aagctaaact tataaaaatg gaaaataaaa taaaacctgg cgtctacgaa cattacaaag
     3601 gaaagcgtta tgaggttatc ggtgttgcgc gccatagcga gacgctcgaa gaaatggcgg
     3661 tgtatcgggc gctttatggc gaggggcaga cgtgggtgag accgctcgct atgtttttgg
     3721 aagaagtgga acgggacgga agaaaaatgc cgcgattcag atatatcgga gacgcaagat
     3781 agcgtcgttg caaaaattct ttttcttttg ctatactatg cgcaatgacc atagatgaat
     3841 ttcaaaaagc ggatcttcgc gtggggaaag tgattgccgc ggagtgcgtg gagggctcgg
     3901 aaaaactttt gaaattacag gttgttatcg gcccgcgcgc cgttgatgta tccgtcgcga
     3961 ttccgccgga aacggaaggt tctggtgttg ccgtggatag cgccgccacg aacgacgtgc
     4021 ggcagattct ttcgggcatc gcgaaaacat atgcgccgga agctatggtc ggcagggagg
     4081 tcgtcatcat cgcgaatctt gaaccgcgga tgatgatggg gatggaaagc cagggtatga
     4141 tccttgcggc gcacggtggg aacggcgaac cggtgcttct cgcggtcgaa cgggaagtgc
     4201 cgccggggtc gaaggtaagc tgaaccaata cgccgttatc cggttcagcg ggagagcccg
     4261 cgcttcccct ctgccattct cctgaagatg aggatctatg cttccattct ttttcttaat
     4321 ccttaaaagc gtggattctt gccctatgga gtaggcgagg ccgccactcc atagggcgag
     4381 ccggagttta tcccata
//
LOCUS       DAIDDT010000059         4388 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_59_length_4388_cov_5.913245, whole genome shotgun sequence.
ACCESSION   DAIDDT010000059 DAIDDT010000000
VERSION     DAIDDT010000059.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59_length_4388_cov_5.913245"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1026 a         1169 c         1094 g         1099 t
ORIGIN      
        1 tcattcgacc ggactgatgg aagtaaaata ataaatgcta ttttttttaa aatatacctg
       61 ttacaataag cggatgttca gtgaatacaa aaacctcgat gatttcaagg aggcgaacgg
      121 ttacgcaatc ggcgaaacat ggtatccgcg cgtgacgaag atcgtcggca taaaagcgaa
      181 gccggcactt tatcattatt acggcgcggc ggcgagctat aaagcggcgc aagaagcgac
      241 cgagcgatcg gccgtcgaag ggacactcat ccatgaagcc gtggaagcac tgatgctcgg
      301 caaagaacca acaatcgatc cgggtatcgc accggcggtg caatcgtttc gggaattttt
      361 tgcgaagggg catatccgcg tgacgcccga attcgtggaa cgccggatca taagccacga
      421 ccaccgttat gccggcacca tcgacgcggt cgccgtgatc ggcggtgtga cgggcgtgct
      481 cgacataaaa acttcgcagg cgatctatcg cgattataat ttgcaaacct cggcgtatat
      541 ggatgcccta aaaaacgagc ttgcggaatt gcaaacgcgc tggatattgc gcatcgacca
      601 gatacaaaag tgcgtccggt gcggcgcgcg ccggcgcata aaaggcggcc gcgaaaagat
      661 tgccgtcgaa tggaataata tgcgcatgcg ggcgtgccgc cacgcatggg gcgacctgta
      721 cggcgagata gaacttcagg aatttccttc ctggcagaat gattttcagg cgttcctcgg
      781 cgcaaagcgc ctctgggaat gggagaacga agagtggttg aagaagacag gatatttatg
      841 attggttttt gcacgaatac aacccaaatg caatgagatt aaaaaatcat taaatgtaat
      901 ttgacgcgcg cttctgtctt gctattattg agtcaataaa gtgccatgag gctccgagaa
      961 atcccgccga tgagcgggat ttcgattttc aaagcatgga tcctcgacca ggtcgaggac
     1021 gacaaagaag aacctggatc caggatattt ttcgcggact caaaattcca ggatgacgaa
     1081 gagagaagtt ctcttcgtca tcttcccgct ctttttcttt cgttctcgtt cagatatttt
     1141 tttctgatgc ggatgctttg cggcgtcgct tccacgagtt cgtcgtcgcc gatgtaatcc
     1201 agtgaatctt caagggaaag cgtgaccggc acttcaagct gatcggcaag gccttcgttt
     1261 ttggcgcgga agttcgtaag ctgcttttcg cgcgcgacat gcaccagaag gtcgccttcc
     1321 ttcgcgcact cgccgaccac catgccttca tagaccggca cgccggcgct gatgaaaagc
     1381 ttgcctctcc cctgcgcggt cacgaggccg taattattcg aaacgcccgc ctgcgtcgcg
     1441 acgatggaac cgcggttgat catcgccatg tcgcccttgt acggctcgta accgagaaaa
     1501 atactgttca taatgcccgt gcctttggtt gacgtaagaa atttattgcg cacgccgatc
     1561 agtccgcgcg tcggaatttc aaagtcgaga aaagcgattt tggcatccac gcgcatattt
     1621 ttcataatgc cgagccgctt gcccatcatt tcgatcacgg tgccggtata ttcttcgggg
     1681 acttcgatgg acaataattc catcggctcg agtttctttc cgtccttttc cttgaaaata
     1741 acctgcggtt tcgatacctc gagctcgaat ccttcgcggc gcattttttc gataaggatg
     1801 gaaaggtgaa gttcaccgcg gccggaaacg atccatgtgt ccgtcgtagc ggtcggcgcg
     1861 acggagagcg cgacgtcggt ttcgagttct ttttccagcc ggtccttgag attccgcgag
     1921 gtcgtaaatt tcccttcttt gccgcggaac ggcgacgtgt ttacggcaaa cgtcattttg
     1981 atggtcggct cgtcgatcgc gattacgggc aatgccaccg gatggtcggc gtccgcaatg
     2041 gtctccccga ttgtgacatc cggaatgccg gcgatggcga cgatatcgcc ggcctttgct
     2101 tcatccacat caacgcgcgc caaaccgtcg aaaagcatga cggacgtcag ctgttctttt
     2161 tttatctcgc ctgcgcgcat gatgtgcgcg atcgtcattc cctttttaag cgtccccgca
     2221 tagagccggc cgatggcgat cttgccttta taattgtcgt acgcaagatt tacggtgagc
     2281 atctgcaaag gcgctgcggc atccacaacc ggagccggaa tgtgttgcac gatcgtctcg
     2341 aaaaccggca cgacatcctt catttcgtcg aggtgtttcg ccagtcccgc ttttccctgc
     2401 attgcggacg cataaatgac cggaaattcg atctgctcct cgctcgcgcc gagctcaaag
     2461 aaaagatcaa gcgccgcgtt gagcgcccat tcgggccgcg cgtccggctt gtcgatctta
     2521 ttgatgacca gaataatctt gtggcccgct tggatcgctt tccgaagcac gaatttcgtc
     2581 tgcggcatgg gtccctcttt cgcgtccaca agtaaaagta cgccgtccac catacgcatg
     2641 atccgctcca cttctccacc gaaatccgcg tggccgggcg tatccacgat gttgatcttc
     2701 gtatctttat aaataaccga cgcgtttttt gaaaaaatcg taatgccgcg ctcgcgctcg
     2761 agctcgttcg aatccatgat gagctcgctc tgcgcaccgg tcttgagctt gaagctcagc
     2821 gactgtttca aaagcgcgtc cacgagcgtc gttttgccgt ggtcgacgtg ggcaatgatg
     2881 gcgatattcc tgatgtccat tttattgaat gatgcgcggt gccggcgaat gatcggcatc
     2941 gcgcgccgat tttttattaa aatttaaaaa cgccacggaa aagcggcgct tcttccttcg
     3001 acgcgaacat tatagcagaa aaaattaaga aacgcaaatt tttatgcagc gaattcaagc
     3061 cactaacaca acgaatgcat ttttatttac ttcctccaaa caaaccgacg aataaaacaa
     3121 agttttataa attttttgta caacccgcgg atcctactta aaaaactgcc tttgagacgg
     3181 gctctaaaca ggattgacaa cactatccat ctccataaaa ttattttttt tctcttgttc
     3241 tggcctccaa gactgagatg gatgctatac cagctccgct tcctcttcaa gccgcacgcc
     3301 gaatttttta tacacttcgg attttgcaag tgtaatgaga ttttttacgt cgcacgaacc
     3361 ggcctcgagc acattcacga tgaagttgga atgtttcggc gagatcatgg cgccgccgca
     3421 ggtaacaccc gcgagtcccg cttcggaaat gagcttcgcg gcggcaatca ccggaaacgg
     3481 atcggtcttt acggaaaacc gcgacccgcg gaacgcaagc gccttacccg caacggcctc
     3541 tttgtacggc acgcttccct ttttatgaat cgaggacagc gggacattct tgaaaatgct
     3601 gccgacgctc ggatagtcga gcggatgatg gtcgttgcgg tgcgcgatat tttttttcat
     3661 cgtttctata attttttttc tgtcgctctt cgcaagccgg agttgcgcgg aaagaatgac
     3721 ctccgcgccg ttgttctttt tgaaaatact gtagcggtat ttgaatgtac aattttttgc
     3781 gcgccgcgtg cggatgacca tccttttcat atcaaagctt cgtaccgaaa caacgcgatt
     3841 ttccattgcg ccgccgaaac atcccgcgtt gccgcggatt gcgccgccaa ccgttcccgg
     3901 aaggccgccc gcccattcga gtcccgccaa tgaccgtttc gcggcaagat taagaagatc
     3961 cgccatcatc acgcccgcac cggcggtcat cgtcattcct ttcacggtta gcgtatcgat
     4021 agcgggcttc aaaaccaatc cgtcccatcc ttcgtcatcg atcaaaagat tcgttccggc
     4081 acccaggata aaaaccggaa gttttcttgc cttcgcttcc gcaaccgccc atacaacctc
     4141 tttttcgttc tgagcggcga aaaagaaccg tgcgcggccg ccgatcctgt aattgctgaa
     4201 ttccgaaagc gaaacgtttt ctttgaattt tggcatatga attctttaaa aaggatgaat
     4261 atcgctccgt ccgacgtccg aatatcggac ggagcgactg ccattttttt ctaagcggtc
     4321 gcgagtggtg acctccaatt ctgtttgtat gaacgagtgc tctgcccgcg accgcttaga
     4381 atcaaatg
//
LOCUS       DAIDDT010000060         4336 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_60_length_4336_cov_5.441418, whole genome shotgun sequence.
ACCESSION   DAIDDT010000060 DAIDDT010000000
VERSION     DAIDDT010000060.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60_length_4336_cov_5.441418"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1195 a         1163 c         1102 g          876 t
ORIGIN      
        1 agaatggaca taacgtcgat attaaaaaaa tattataaaa aatcttccca tgttcggaaa
       61 aaatccattc gcggaggtga gaaaaagaga cgggcgcatc gttccgttcg ataaaaacaa
      121 aatcacgggc gccatcgcgc gcgccatgca ggtgacgggg gaaggcgacc cgaagcagga
      181 tgccgaaaaa gtgtccgaac aggccgtcgc cgcgctcatt aaaaaatttc cgaaggatca
      241 cgttccctcg atcgaagaga tccaggacat cgtggaaacc cagctcatca tcatggattt
      301 cgcgaaaacg gcgaagtcct atatcctcta ccgcgccgac cgcgcgcata tccgcgaatg
      361 gaaacgggag atccccgaac gggtgaaaac gcttgtcgca gaaagcaaga aatatttcag
      421 gaactcgctc gctgaattcg tctattaccg cacctactcg cggtggatcg aagaagaagg
      481 ccggcgcgaa acatggatcg agaccgtcga ccgctacatc gatttcatga aagaaaatct
      541 cggagggaaa ttgacggacg ccgaatacgc ggacgtacgc gaagcgatcc tccgccagga
      601 agcgatgccg tccatgcggc ttttgcagtt cgccggaaaa gcggcgcgcg cgacgaacat
      661 cgccgcgtac aattgttctt atatcgcacc ggaaaaattg gaggacttcg cggagatcat
      721 gtacatcctg atgtgcggca gcggcatcgg tttctcggtg gaaagccaga ccgtgcaggc
      781 gctcccccag ataaaaaaac agaacgggaa aaagcttccg gcgcacgtga tcgaggacag
      841 caaggaagga tggttcgacg ccctggcggc cggcctcaag acatggttcg acggcaaaga
      901 tgtcgatttc gatttttcac agctgcgtcc cgccggcgcg cgcctgaaaa cgatgggcgg
      961 caaaagctcc ggtccggaac ccctgcgctc actcctctct ttcgcgcgaa ggaaaatatt
     1021 cgcgaagcag ggcagccggc tttccaacgt cgacgtccac gatatctgct gcaagatcgg
     1081 cgagatcgtc gttgcgggcg gcgtacgccg cagcgccatg atctcccttt ccgacctcga
     1141 tgacgtcgag atgcgcgccg caaaaaccgg ccaattttat atgaccgaac cgcagcgcat
     1201 gatggcgaat aattcggcgg tctacacgca caagcccgac gcgacgcaat tcctcgatga
     1261 atggcttgcg ctcgcgaaaa gcagggccgg agaacggggc atcttcaacc gcggcggcct
     1321 tgcgacgcaa atgccggaac gacgcgtaaa agcgctcaaa tcggcgatcg accggatcgg
     1381 cacgaatccg tgcggcgaga ttctcatcca atcgaaggaa ttctgcaatc tttcggaagt
     1441 cgtcgcgcgc gcggaagaca ccgaagcggc gcttatgaaa aaaatgcggg cggcggtcat
     1501 cctcggcacg taccaggcaa cgctcacgaa tttcccgtat atctcaaagg aatggaaaaa
     1561 acatgtcgag gacgaacggc tgctcggcgt ttccatcacc ggccaatggg attgtcccgt
     1621 cgtccgcaac gaaagcgtcc tccgcaatct ccgcaattac gcgattgaag tgaacaaaga
     1681 atacgccaaa cgcttcggca tcaacgcggc ggcggccatt acctgcgtga aaccgtcggg
     1741 aaccgtttcc cagaccgtgg actcctcatc ggggcttcac acgcggcatg cgcaatatta
     1801 tatccgccgc atacggattg cggcgaccga cgggcttttc cagatgctga aagaccagaa
     1861 agtcccttac ttccccgagg tcgggcaatc gctttcttcg gcgaccacgt tcgtcattga
     1921 atttcccata aaagcacccg acggcgccgt cacaaaaaac gaccttaccg cgctgcagca
     1981 gctcgagtac tggaaaaaag tgaaaatgaa ctataccgag cacaatccgt cggtcacgat
     2041 ttccgtcggc gacgacgaat ggatcgaagt cgcaaactgg gtctatgaaa attgggacat
     2101 catcggcgga ctttctttcc ttccgcgcga aaacgacgac gcggtctacc agctcgcccc
     2161 gtacgaagaa atcacaaaag agcgctacga ggaaatgacc gcaaaaatgc cgtcgatcga
     2221 tttcgaaaaa atcctccttt acgaaaaaga agatgcgacc gatgtcaaaa aagaactagc
     2281 atgcgtaagt ggcgtctgcg agatagaaga cattcccgcg aaaacggcgg ctgtgtaacc
     2341 ggactgaatg aagtcataaa aaacgctctt ttcaaagagc gtttttttat ccgtatttta
     2401 ggcgaatcct ctcacgcccc tcttttccgt cgtccttgcc ctgtggagta ggcgaagccg
     2461 ccactccaca gggcaaggat ccgcgctttc tgaatttaaa atgggaagta tggattccat
     2521 tctttaaggg gacgacagag gggaaagtgc ttcggtttcg acagaatcgg cgctgattat
     2581 agatagtgag gggctacacg gcgacttatt tccaattccc ctcaattttg atagaatgct
     2641 atcattatga acctcaagaa agctcttatc atcctcggta cggtcgccgt cctgctcggc
     2701 atcgccgcgg tgttcgccgt attctataat ccgtcgattt ccgtcatacc gggcgttgcg
     2761 tcaccgtcat caacctctct tttttcgctc accaaaggaa cctcgggtat agcatcggcg
     2821 ggaaacgcaa ccggcagcgc ggcggatgcc ttttttggaa ataccccggc ggggggatcc
     2881 gcgtcttcca cgggaagcca atcaggtacg ccgcaatttg caagcacgta cgcatggccc
     2941 tatcccctgc aatggacgga aggacagtca acgctcgtga tcgccggcgc cgccattgaa
     3001 gggaatcagc tcgtgcttgc gatcgtgacg catataggaa gccagcagga atgcataccg
     3061 atgaacgtag accttgtcgt tgatgaatcg ggtaatacgg aaagttcgac gccggcgcaa
     3121 ttttcatttc ccgataccaa tagctgcaac gggacgccga acacgacgta tgacgcgcaa
     3181 aatttttctt ttaatttgaa cccgaatatg caagcgcctt ttcttctcac gaccggcggc
     3241 gcttcgaata ttttcttcga agtcgcgacc acgaccgacg gcgacgtgac catcacgctc
     3301 cccgcaacaa cggactagaa cccgtctcaa aagcaggccg gcgggttctc ttccggttcc
     3361 cgtgtccgcc tcaaaacaaa cgcggcacct ctcttttttt tattttttca ccgataaccg
     3421 caccgataac cattaacctt tcgctatcta taaatcagcg ccgattctgt cgaaacattt
     3481 tcccctccgt cgcccccccc cgaaggaagg aatccatact cccgatccga agatttcaag
     3541 aagatttcaa agcgcgaatc ctcgaccgga gcctgccctc gacccgatcg agaaggacgg
     3601 aaaagagggg cgtgagaggt tacgataacc ttactggacg gcaaaaaaaa gatgcgctaa
     3661 tattctaaag attccgcgat agctcaatgg tagagcggcg ccctgttaag gcgatggttc
     3721 ctggttcgag tccaggtcgc ggagcactta ggaatatact atctctatgg aaaaatcttt
     3781 cgagtccgag gaagcttcgc gggaaaacga aaaagaagaa aaagaaacgc tttcaaataa
     3841 agagaagttt gaaatcattc taaaactttt tggaacggat aaagcccgtg acatttttat
     3901 tgaaaattgc aaaaaatacg ccgatgcgcg aatagcttcc gcaaggaatg cctatgtcca
     3961 aaatcatggc gaaaattata tatcgctgag ggatcgttat tccccgccga aacgcgccca
     4021 ctaccataat gaaattatgt acacccttaa taatctttcc cttcaaaaac ttgaccccat
     4081 tcaagaaatg gtattaaagg aaatgagaag cagtcgcgaa acggcgtggt acatcatcga
     4141 ggattggcta ctgtcacaaa aagccaaaaa aacagacgag gaaaatgata ataaagaacc
     4201 cgatacgtgg ctgtatcctt cgcggcgaat agaagaatag aagaatagaa gaatagaaga
     4261 atcggatacc ggtttccgcg ttttgcgcgg agcatcaaaa gcaaaaaacc cgtggcgaag
     4321 ccgcaggaaa ttataa
//
LOCUS       DAIDDT010000061         4141 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_61_length_4141_cov_6.551181, whole genome shotgun sequence.
ACCESSION   DAIDDT010000061 DAIDDT010000000
VERSION     DAIDDT010000061.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61_length_4141_cov_6.551181"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          881 a         1061 c         1092 g         1107 t
ORIGIN      
        1 atcatagctt atctttaatt aaatgtcaaa tccttggacg cgaccaacct tgctaccatt
       61 tttccgccat cagtattttt tccgggcgga atttccgcca gacggcttcc gtgacgaacg
      121 gcatgaaagg atggagcagt ttcatggagc cgaggaggag cgactcgagg attgcgtatg
      181 atgccgcttt ttccgcttcc gtgccgttct ttaaattttc tttttccgtt tcgatgattt
      241 tgtccgcaaa cgtgtgccag atatagcggt acattttttc cgcgccgagg tggaactcga
      301 aattttcgat atgcgccgtc acttcggatt tcaatgcttc aaattctttg agatatattt
      361 catggcgctc gttttttttc atttctccgt tttccgctct ccattctgaa ttctgcatta
      421 tgaaccgcgc gatattccat attttcgtcg aaaagttccg gtagccgcgg atgcggtctt
      481 cggaaagctt gatatcgttg ccgggcgcag cgccgatgat gagcgaaaga cgtgtggcat
      541 cggcgccgta cttgtccgcc atttccaaag ggtcgatgcc gttgcctttt gatttcgaca
      601 ttttccgccc tttcgcatcg cgcacgaggc cgtgcagata cactttatgg aaggggactt
      661 cgcccataag acacgttgac ataaggatca tccgcgcgat ccagaagaaa aggatgtcgt
      721 agcccgtctc gagtacggaa ttcggaaaat aatttttcag atccgctgtt tcgtcgggcc
      781 aattgaatac cgagaacggc caaagccccg acgagaacca ggtatcgagc gtatcgggat
      841 cctgttgcca gccgtcgcct tccggcggct caatgtcgac gtagaatttt tcaggctgtt
      901 ctttggctaa atccgtctgc gtcaacaaag attcatcgtc gatatcctta taccatgccg
      961 ggatccgatg cccgaaccag atctgccgcg aaacgcacca gtcgcggaga ttatcgatcc
     1021 aattgaaata gatcttctcg aagcggccgg ggagaatttt tactttaccg ctttctacgg
     1081 catcgcgcat taattctttc aatgttcttt cttcgtcttt ttcattgaaa gcgcggatct
     1141 ccgccttcgc ggggacggca tcggaatgtc gttttccggt ttttgccgca aacggcttgt
     1201 tcaccattat gaaccactgc agtttcggca gcggttcgat gatgccaccg gtgcgttcgg
     1261 ccgtcgcgac attttgtgaa atttcctctt ctttttcgat aagattcgcg ttgcgcaaca
     1321 gtgcaacgac cgcttcgcgc gcttccgcgg tttttttacc gtataccgtt tcgccgccga
     1381 ctgtcatctt cccgtattcg tcgatgatct gtttaaaggg gaggccgtgc cgccgcgcga
     1441 tttctccgtc gatcacgctg tgcgcgggcg tcacgcccaa cgcgcccgtt ccgaaatctt
     1501 tttcaaccga ctggtcggcg atgattttaa tgtgaagcgc tgcgccgcag aatgccacat
     1561 cgtattcttt gccggcgaat ttcttatacc gttcatcgtc gggatggatt gcgacggcca
     1621 cgtcgccgaa tttcgtttcc ggccgcgtgg ttgaaatggc gacagggaag agtttatctt
     1681 tttccggacc gctcaggatt ttcccgtagc ggaacgtata gagttttgtt ttccgttctt
     1741 catagacaat ttcatcgtcc gagatgacgg tttgaccttt cgggtcccaa ttcaccacgc
     1801 gaaaattgcg ctcaatgagc cccctgtcgt acatctcctt gaatgccgtc cggacagcgc
     1861 ggctgcgcgt ttcgtccagc gtatatgctt cgcggctcca atccaaagaa gcgccgagcc
     1921 gttttatctg ccgtacgatc gtatcatggc tttcctgcgc gaacgcgttc acgcgcgaga
     1981 ggaattcttc gcgcccgaga tcgtggcggt tctttttttc ttctttcaat atctttttct
     2041 cgactttcga ttgcgtggcg atggcggcat ggtcggtacc gggcacccag agcgtccgtt
     2101 tgccgttcat gcggtggtag cgtaccatga cgtcctcgac ggcaagcatt gccgcgtgcc
     2161 ccatatgcag ttcgcccgtc acattcggcg gcggcaatat aatagagaac ggttccgcgt
     2221 ccgccgccgt cactccgttt tctgcgcaga tatcggggtt gaaatatccc gactcttccc
     2281 agagcttata aatcttctct tcgcttttga cgtggtcgta gcggggttcg aattcgtttt
     2341 cgtccatacg ggtattgtaa gcgatttttc aggcgtttgt catctgtctt cgtttgctat
     2401 cctgtttttg atggttgtta aaaacaggat agcaaacgaa tatgaacgct ttcgcaaacc
     2461 gataaatagg aaggtataaa aatactttta tcgaaacaat cgttccgata aaagtatttt
     2521 tgcattgcgc gtattaaata tccagatctc ctttcgcggc gagcttatat ttttttttgc
     2581 ggaggaacgc cataaagccg gcggtggcaa tcatcgcggc attgtcctgg ttatacgcgg
     2641 cgtcgggaac gaaaaacgcg cagccgatcc tttcggattc tttcttaagc gttttccgga
     2701 gaagtttgtt cgacgcgacg ccgccggaaa gcagaaccga tcgcgcaccg tattctttcg
     2761 ccgcgcggat cgtttttgcg gcaaggacat cgacggcggc ctgctggaac gaagcggcga
     2821 catcggcgcc gtccgcattt ttatggctgc gcaaataata gagcacggcg gtcttcagtc
     2881 ccgaaaagct gaaatcatag tttttgtcgc gcatcatcgg ccggggaaaa tcaatcgcgc
     2941 gcgcgtcgcc cttgcgcgca atcttttcta tttccgggcc gcccgggtag gggagctcca
     3001 acagccgcgc aaccttatcg aatgcttcgc cggcggcatc gtcgcgcgtt tcgccgagcc
     3061 gtttccattt ttcgaatgta tccatcttga ggacgatcgt atgtccgccg cttgcaatca
     3121 gagcgacggc ggggaaaaga gccggacggc gcgcggcggg tgatgtacga tgcgcgggaa
     3181 gtaaaaaact gtacaggtgt ccttcgaggt ggtttgcgcc gataagaggt ttcttcaatc
     3241 gtgcggcgag ttcttccgcg aaattgatgc cggtccagag cgccggttca agtccgggcc
     3301 cgacggtgac ggcgacgaga tcgacggaat tgagcatcga gcatcgagca tttagttttt
     3361 tgaggagcag cggaagattt ttgatgtgtt cccgtttcgc gagattggga acgacgccgc
     3421 cgaacgggcg gtgaatcttt atctgcgacg caaccagact ttttaaaatt ttgaatttcg
     3481 gattccgcaa tccgcccgtc gcttccacca gggcaacgcc ggtttcgtcg cagcttgttt
     3541 caatggcgag aatgcgcatg atgtttttac ggataaaacc cggcggtcgc aaaatttgcg
     3601 tgcacgccga tctttcttta ttcttccgac gattttttat gccggggcgc aaaatggata
     3661 tgcataaaac tgtcgatgcc gaggtagctg ccggcgttcg cggcgaccaa taccaaaagg
     3721 catacgatga taaggatttc gtcattgata aattgcgacg cgctcacggc ggaaaaattt
     3781 acagtgggta aatagcccgc aagcgtgagg ccgatgccga taatggacgc gagacgggta
     3841 aagagcccta tgacgaggca cgcgccgatg ataaggaatg cccactggaa atagagctgg
     3901 acatggctta ccgggattgc cgtcggcgcg ccgctgctca gccattgata gaggcttgcg
     3961 aacgatcgcg cgccgagttc acgtccgagc gggacgttaa agatcgtact gaagccgaga
     4021 taaaaaaaat tcaaaccgag cgtgagccgc aggaaaaaaa tgacaagccg cagggaatgg
     4081 gaaatttttg atttggcggc gtttttcatg gatgattatg gtacaatgat aacattataa
     4141 c
//
LOCUS       DAIDDT010000062         4014 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_62_length_4014_cov_6.456693, whole genome shotgun sequence.
ACCESSION   DAIDDT010000062 DAIDDT010000000
VERSION     DAIDDT010000062.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62_length_4014_cov_6.456693"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          972 a         1122 c          876 g         1044 t
ORIGIN      
        1 aatcagtagc gataacgctg ctttgaatgt tttttcatcg gtcattccgg aatcggcaag
       61 atgtcttttt aattataatg tcttttttta gaaaactgaa agtcttcctc tttaggaata
      121 cttcaacaaa gcagacggtc gcaaaaaaca ccgtctggct ttccgtctcg aacttcggag
      181 ggcgcgccat taaagcgatc atcgtcattt acgccgcgcg cgtcctcggc accgccgcat
      241 ggggcgtgtt ttcctacgcc gtaaccctcg caggattctt cacctcgttc ctggatcccg
      301 gcgtgaatgc catcctaatg cgtgaggcgc caaaagcgga agaaaaagaa aaaatgaccc
      361 tgttttcgac cacgctcgtc atgaaactga cgctcatcgc cgcaacggcg gccttcatca
      421 tcttcgtggc gccttccttc agcacgcttc cgggcgcgaa aatactgctg cccatcgtcg
      481 ttcttatcat cgccttcgac acgttgcggg atttcttttc cgcattcatc cgatccatgg
      541 aaaaaatgga atgggaggcg ggcatctttc tgtttaccaa cttgagcatc gtggtcgccg
      601 gatttatttt tcttgccatt gcaccgacgg cgcagtcgtt cacttgggca tatgccgccg
      661 gaaccgccat cggcgccatc gccgcaatat tcgtgctccg gcgctatctc aaaaaaatat
      721 tttcattttt ttccattcgc ctcgtaaaac ccatcatcgc tgcggcgtgg ccgttcgcca
      781 tcacgggcgc gctcggcgta ctgctcacga acaccgacat tcttatcgta agctggatgc
      841 gcacggcatc cgatgtcggt atttattcgg ccgccatccg catcatccag gttatttatc
      901 tcatccccat gatcttccag ttcagtatgc tccccctgtt cgcccgcctt gcgcatcgcg
      961 ataacgaaaa attccgcatc ggactcgaaa aaaccatcgg gttgatattc ctcgtttcgg
     1021 ttcccctcgc gctcggcggc gcgatcctcg gcacacagat catggcgctt gttttcggcg
     1081 catcgtatgc cccgggcgga ctttcattca aaatccttat gctcacgatg ctcgttgatt
     1141 ttccgtcgtc cgtcatcagc gccgcggtct tcgcctacgg ccgccagaaa gggcttatca
     1201 tcacctcgct catcggcggc atcggcaacg tcattttcga tattctgctc atcccgcgtt
     1261 tcggcatcgc gggttccgcc gtggcgacgc tgctcgccca gaccgcaagc aatgggtacc
     1321 tctggcacat gatgaaaaaa ataaaccgct ttgaggtgct gccgaagctc ggcctcatag
     1381 ccattgccgg cgccggcatg gccgcaatca cggcatcgct tttcgcgctc catgtcgaag
     1441 tcgttctgaa cgtcgcgctt tccgccgccg tctatcttac aatcctcgcg gcgttccgcg
     1501 aaccgctgct cgtggaaata aaacacatcc ttcgtccagc agcggaagtc gtctccgaat
     1561 cgattaaagt ataaaacccg tcccgaacga aaaacggcaa agcgagctta tttcggtgca
     1621 aatccaaacg gtttttcttc cttttcaatc ggcggatcga taagctgttt gagcattatg
     1681 ataatattct gcaggtcgga atcgtgcgcg ccaactttat tttccaatac ttctatctta
     1741 agagcgagtt cgctgttcat ggcgaatact cgccgcattc tcacaaaggc gcggacaact
     1801 tggatgcttg cgcgaatcgc tatggagctg ttcagtacgc ttgccagcat aaccgcgcca
     1861 tgctccgtaa acgcatacgg cgcgtatttc aaatttttac ctttcttcaa ggtcgcaaat
     1921 tgcgaccttg aaaccaacaa cgccattcct tcctccatgg tcaattgaaa catgaagtca
     1981 tcggggaagc ggtctctgtt gcgtttcacc tgttcattga ggcgcttcac cgaaacgccg
     2041 taaaattcgg caatatcacg atcaatcatc actttatggc tccgcaaaac aaatattttg
     2101 ctttcgataa tcttaaacgg ggttggcgct attttattct ctgattcttg catgccctca
     2161 ttatcacaca aaggcattaa aattttatta aaaccagcat catactttta aataaggctg
     2221 cgataaaccg ctaaaaatct atcatatcaa aaatgatacg ataggccgca tacatttgat
     2281 acgattttat atgataaaaa aatattaaac acgcggcatt atatcattct tgcaacaatc
     2341 accgcgaaga aattccccgt ttcaatttcg acagaaagcc gtgcgacatc cggcggacgc
     2401 cctccggcag aagactgtag caatattgcg cgaggttcac cgcataagcc ggataaaaat
     2461 tcggatattc ttttttatgg gcgcggacta tccgcaaaat ttccttcgac tgcgaccgct
     2521 tgaaaagaaa cgacttactg ctttccgcca tgagataccg cacgaaatat tcctgaaagt
     2581 tatggaactt tccgcgcgcg cccatcgtga gccacaagtc ccagtcctcg gcgttcttga
     2641 aatcgccata gccgccgact gcaagcgcaa catcgcgacg gaacatcacc gtagaatgcg
     2701 tgaaaggatt cgcgaacaac gcccttgaac ggatcgccgc atcggtctcc agtttcagat
     2761 accggaaccg ctcgcgatca ttctcgtcaa tgacgatagt cccgccgccc gtgacgacgc
     2821 agtcaggatg cgcgtccaaa aacgccgtct gttttttaag tttatcaggg tcgcaccaat
     2881 aatcatcgtc atcaagccgc gcgatatatt tcccctgcgc cagtttcaat ccctcgttca
     2941 gcgtccgtga aatgtcggga taatagttcc gttcgtgatg caccggccgc acgcgggcgt
     3001 catgctttgc caatgcatcg agatatgcac gggttccgtc cgttgacgcg tcgttcaaaa
     3061 cgataagctc ataatcggag aacgactgat caagaacgct ccgcgccgct ctttttaaaa
     3121 ggtcgactcg attataggta gggagcaaaa tactgactac gggagattta tccatgttat
     3181 tgcaacaaga aaaaccggcc ttattatttg atcgcctctt caaactgccc catccacacc
     3241 gaaagcatcg cattgccttc ttttcccaca aggtactgcg aaagaatttc gcgggcaacg
     3301 gcatgttcat ctataacggc attgcctttg cgagctaccg ccacgacatt cggcaattta
     3361 tgtccccctc tttttttata aaaagatgcc ggcaaaaaat ttcccgccat gttcaaaaat
     3421 ggaactttcg gaaaatgctg cgccacggca ttctgatagg ttttaatgac gactttttcg
     3481 aaaccgcatt ttccaaaaac atattcgaac ttcggcgcat cccaaaaatc gtaatggacc
     3541 gcccacggcg cttcctgcga tcccagcatg tgccgtccca gctccatgcg ccttttttgc
     3601 gttaaagcgg acgcataggc cgcggcgcaa ccgtaaaaat cgggcgtttc gataacgagc
     3661 tttccgccct gcttgagcca gcggcgccat ctcataagaa ttgcgactgc ctcggcacgc
     3721 tcgaaatgct caaaaagatg gtgactgcgg atctcgtcga ccgaattgtc agtataggaa
     3781 agcgtgcgga tatcatgata aacatccgcc ctcaccttca taaccgagtg ctcggattgc
     3841 ggatagtcta tgtttacata tccttcaaga tatttttcgc cgcacccgat atgtaatttt
     3901 atagcaggtt ctttttccat aatttatttg aatatttttt catgaatgcc gccgcaaatc
     3961 ctttgccata cttgaagaat aaaaatattt tagcatgaaa cggctggcca tagg
//
LOCUS       DAIDDT010000063         3979 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_63_length_3979_cov_5.896463, whole genome shotgun sequence.
ACCESSION   DAIDDT010000063 DAIDDT010000000
VERSION     DAIDDT010000063.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63_length_3979_cov_5.896463"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          893 a          987 c         1023 g         1076 t
ORIGIN      
        1 gatatctgtc cgttcaatta ttatcggtcg gtgcgataaa aacggattgt cccgtttcct
       61 tgcagtcgca ccacgtgggg cgggtatgag aatgacggaa tacttgaccc gggatatggc
      121 cctgcgggat tcgacccttc ataccagaat gccacccatg atttcggatc atttccgacc
      181 atctgttttt gccagctttg ccatccatcc ggcgtgcgaa tccggcccga ccagcatcct
      241 ccgtgaagtt ttatatcgaa gtaatcgccc ctgaaggatg aaaaattaag tttactttcc
      301 tgtctccatg cgtcgtcgca gacgcgcgcc ggattgtaat ttcttctcat ggattgatgt
      361 tcattgccgc cgccagccgg tatcattttc aagatgccga taagggataa taccgccgag
      421 atgattattg cggagataaa aaattttccg tttatcagcc ggccgcttcc ggttggtatc
      481 ggtatttttt gcataggcat tattcctcac cccctttttt cttgaccatc gccgagaaaa
      541 tccctttgag aaggtcttct cctgcattaa tcaattcggg gctcatctca atccttgaaa
      601 cctttatatc ctttttgaca agacttctcc tgatgagggt ttgttccagc ggatctttga
      661 ctccgggaat atccgcgtag ccggccgctt catctttaac attcttcgca atgatatcct
      721 gctgttccgc gttgaatttt tcggccgcga cctttgccac ggcaactttc gtttcttcgg
      781 tcggttccat attgccgacg accatccgtc gtatgacgac gcccagcccg tgcagcggga
      841 atgattgatt tccgtccctc attttttgga tgagtttgaa ccgttcctga gtcgctttgc
      901 ttaattcttt tttttcgtca tccggcagtt ttttgatagc tttatcccat tgtttttcca
      961 atggacttaa cgccatcctg cctttgaaat atccaatcac agcttcggtt ggaatctccg
     1021 gatggattct tttcacgtcg tcggaaagca gtccttccag aatatggatg atggcttctt
     1081 cgttcatccc tcgggcctga tccaggttct gcggcccttc cgttttggat acgatccaat
     1141 tccgaaagcg ctggctaacc tgttcatcta tccggtttcc gacttctccg agtccgccca
     1201 cggttaaaac ctggacggga ttttggggat cgactgagat gtagatgctc actggaacag
     1261 ccacttcact gcgatcgccc ggcaccattt cttttatggt gaaccggacc gcgacctctt
     1321 ctccggttat ctcctggaaa tcaaggacta atcctttcaa tggaacgaag tgccaccccg
     1381 atccaaggag gttattcact tctttttccg ggtcgataaa tttcccccat atcataggca
     1441 ttcctttcgt cggcggattt gccgggatga catagatgct cctccggaaa aattcctcaa
     1501 agagtacgat gaaatttccc gctgcgaacc aaaatccgag cgctgtccaa acgtaataga
     1561 cagacgtgaa gatcaagaca atggcaacga atgtcaccaa cgccctcatt gcattaaact
     1621 ttcccatatt acccacctcc tgttatttag tttttgcgcc cacgcgccac tgaataaaag
     1681 ttagcataat ataaagaaaa tgtcaaattt tctgcgcggt aagccggaaa gaaagatagc
     1741 ccctttatca tcgcttcgcc ttccttctct cgtcgtccgt gaagaaggat aacaggcggg
     1801 gattaagtgt cccttctttt tccgtggaat gctttatgga cttcacggag ggctttgtcg
     1861 gtgagatggg tgtaggcctg cgtggtcgaa atattggaat ggccgagcag ttcctgcacg
     1921 gagcggaggt cggcgccgtt catgaggagg tcggttgcaa aactgtgccg gagctcatgc
     1981 gcgcggacac gctgcgggat gccggcttcg cgcgcgcgct tcgcgattag gcgctggacg
     2041 gcgcgtggcg tgactctacc gatgactttt cccgatttat cgatgcttac gaacagcgct
     2101 tcttccgcgt cgccgcgttg cgcaagatat ttttgtatcg cttgttttgc ggaatcggcg
     2161 ataaaaacga cgcggatctt atcgccttta ccgcggacgg aaacttcgcc gcggcggaga
     2221 tcgatcgtgc ggtcgagcgc gcagagttcg gaaagccgca aaccggacga gaagaacgtt
     2281 tcaagaaccg ctttatcgcg gagcgcgcgg agtccgtcgc ccgcgggcgc cgcgagcagc
     2341 cgttcaagat cgttgtattc caggacttcg atctgccgcg ccggcgtttt gggaagctct
     2401 attttttccg ccgccagcgt tttgatgtcg cgcttcgcga gatatttaag gaagttccgg
     2461 atggcgatga tgtaatagct ttgcgtgagc ttttttaaag aacgggcttg tcccaggttt
     2521 tttttctccg aatttttttg gtttgccctc ccgttttttt ctccggaaaa ttttggaggg
     2581 cgggcgagcg cgatgcggaa tccgcggacg acgtcatccg tgatgtcttc cgccgccgca
     2641 atctttgaaa aatcgagaaa tacgtttaaa taatgtccgt aattctcgcg cgttttcggc
     2701 gaccggtttt tttcgatttc aaggtaatca aggtaatctt tcagaagttt ttttatttcg
     2761 gtttcgggca tgattgtatt ataacccgaa atacggcgtg tgcgatcatc ggttattgat
     2821 aagcatccgc ctcaaaagca tgccggcggg gattgcggac gattgtggat tttttcattg
     2881 ccgtaatttg cgtttttggc aacgctctgc tataatgcat ccatgcttac caaacgggtc
     2941 aaatcaaaaa tagcgaaaga gactggaaat cacgagagtg atacgggttc cgccggcgtg
     3001 cagattgcgg ttttgtcgcg ccagattgac gaacttgccg cgcatcttaa gaaaaacccg
     3061 aaggacaatc attcgcgccg cgggcttttg aaaatggttt ccaagcgcag gaagttcacg
     3121 gcttatatga agaaaaacga accggcttca tacgataaat tagcgaaaaa actcgatctt
     3181 aagcgataag ggtttttacc gcaaaattgg catgaagcag ggtcagaatt atccaagcca
     3241 gcgcgtcggc attttcatcg atgtggtgaa tttataccac tccgcgaaaa acatctatcg
     3301 cgggcgggtg aattatgccg agctgatgaa gcatctcgtc ggagggcggc agcttgtccg
     3361 cgcaatggcg tacgtggtga aaagcgaagg gattgaagcg cctcacgcgg agggcgcgca
     3421 tgttcgtccg gcgcgcgtcg atgaggcgcg ttcggcggcc ggcgtcaatc tttcttccga
     3481 agccgcgttt tttgaagcgc tggagaaagc gggccttgaa cttcgcgtga aggacctgca
     3541 aatttatgcg gacggcacga aaaaaggcga ttgggatgtc ggcatggcag tggacgccat
     3601 ccggcaggcg ccgtttctcg atgtcattgt tctcgtgacc ggcgacggcg atttccttcc
     3661 tctcatcgat tacctgaaat ggggtacggg acggatggtc gaggtggcgg cgtttcatcg
     3721 aagcgcgtcc gcgaagatac aggaagcggc ggataagttc atcaacatcg aggagattcc
     3781 gagaatcctc atcccgacaa gcgcgcggcg cgtcgggagg cggtagaaca ggaagagttt
     3841 gttttatcgg gacaatcccg cctgagtttt cctctgaaaa actcaggcgg gcagggcatc
     3901 cgttttttga ataaaaaata aattaaagtc gtaccgttta aggaatatgc gtgaaaaaat
     3961 aaaaaataaa ttacaatga
//
LOCUS       DAIDDT010000064         3805 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_64_length_3805_cov_5.301234, whole genome shotgun sequence.
ACCESSION   DAIDDT010000064 DAIDDT010000000
VERSION     DAIDDT010000064.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64_length_3805_cov_5.301234"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1061 a          979 c          899 g          866 t
ORIGIN      
        1 ttcaagagaa aatggaaaga acgcgccgtt cttttcctat gggcaaccca tatgaaaaaa
       61 tggcgttccc ctgctccggg ggataccgtc tctgaccttt atatatggga tggaaatcat
      121 gtgccgcttg gtgaatggat tattgaaaac aggacgtacg cactggcgcc gttgctgctt
      181 gagatatttt cttatgattt ttttcaaatg cgcacgtcct atgaaaaaaa aaataaaaca
      241 aaaagcgccg ttattccgta aagccaacgc ttcttctttt ttttaccgcg gcaaggggat
      301 tttatttata aaaatgtcgc cgcattacga tcctgctcct gaagagtttt cgcaaaaatc
      361 gtttttttcg tctttgggtc gctgatataa aaccaatacg gagaagattc gggatgcatg
      421 gccgcttcta tactcaaaac acccggattc cctataggac cagggggcat ccccccatac
      481 aaatagctat tataaggcga gtcaattttt aagtcaagag gggtcagcgg agaacatccg
      541 gattccgaaa gcggagccgc catcatcttc gcatagcaca ccgtggcatc gacttgaagc
      601 cgcattcccg ccgcgagccg cttcagcaat atccccgcaa ctatcttctg gtcttccgga
      661 tcgggcacct ctttttcaat gatggacgcg aggataagat tcttttcgca taccgcatcg
      721 gacgcgcact gcgcgtcatt gaaaccgagc acgggttccg ccttggcatt aaaatcatcc
      781 aaaaattttt ggacgacggc ggcgaccggc ataccggtga aaaattgata ggtatcggga
      841 aacagccttc cttcaagatt cccgtcggca tgaaaattaa tgagatcgcc gcgatggatg
      901 accagcgcat cgctcagtat cttatcgatc tcataaatat tcgatccttc cggtaccgtc
      961 accgtaaccg gttgcagcgc gctattactc gtcaatacat cctcgatagc gggcgccccc
     1021 atcgcgcggg tgagctgata atcccccggt ttcaatgccc ctgctcgtcc ggaaaccaat
     1081 aagagaatat cgaaaccgac cgacgaacgg ataaaaccgg cctgataaag atccctgctc
     1141 acttcgcgaa atccttcacc cgatcttatt tcaaaaacag gcggcgcatc tcctttttgt
     1201 gcgccaaccg gcgtcaaagc ataaaaaaaa ccggcgcatg cgagtaaaat aagaaacgtt
     1261 gccgccggcg cgaaaattat ttttttgttt gaaaaattga tattcataat tccgacacaa
     1321 atagttgtaa tgacgaaatc tcgcaacccc tcacgctcct cttttctgtc gtcctcgcgg
     1381 aggcgaggat ccacgctttg aaatctcctg atcggaaata tggatcccct ccttcctggg
     1441 ggacgacaga gaggaaacgc ctcactctaa tcgatacttt attataggca gtgagggatt
     1501 acgcaatact ccatcgaccg aaactgcctt attataacgt tatattttac tattacaaca
     1561 atatgaaata aaatttcata tcttgattcg ttcgaaaaat ataaatatta agaaatcttt
     1621 acgtttttct cgccgccaag attaaaacat tacaacatct atagcgcttt acgaacttcc
     1681 ccgcgagcgc tgccgcaccg aatttacgac cgccagtagg aaaaagtcgg cgatgatgct
     1741 cgatcctccg tagctcagga acggaaatgt gacgccgaca accggcgtca atcccgtcgc
     1801 ggaaccagcg ttcagaagga actggatacc gagcatcgcg gcagtgccga gacagatgaa
     1861 tttctcgaaa ttcttgtcgg cgcgaagccc tatggcaaga atccggaaaa taagaaaaag
     1921 gaaagcggcg atgataagca ggcctgccgc aatgccccat tcctcgatga ttgccgcaaa
     1981 tataaaatcc tccgtcggct cgctcaaaaa cccgagttcc gtctgcgtcc cctgcccgta
     2041 tccttttccc caaaatccgg cggaaccgat ggcgattttc gtctggatcg tgctgtaatt
     2101 cacgccaagc gagctttgct gcggagaaaa ataaccgatg atccgcgcgc gatgatagtc
     2161 cttgagaacg taaacccaga ggaatcccgc caccaaaata aaaatcgccg atgccgcaag
     2221 gattcttcgc gcgggaagcc ccgaaagaag caggaaaccg aaccagatgc ccgcaaaaat
     2281 aaccgcggaa ccgaggtcgg gaagttttac ggcgatcgcc gcgggaatga tgaaaaagaa
     2341 aaatgacgag aggatagtcc gccatcgcgc cacggcgaga tgccgccggc tgaaataatt
     2401 cgcaaaaaga aaaataaggg cgatcttcac aagttcgacg ggctgtagag tgacggggcc
     2461 gagcgaaagc cagcttctcg tgttccgtat gacagggcct ctcgcataag caaggatcat
     2521 aagggcgagt cccgccgcat aaaacccccc gatgacccac cggacgttaa aaaccgaccg
     2581 ccagtcgaca aaaagaaaaa gaacaaccaa tgccgttccc agcgcgaccc agatgagctg
     2641 cataaaaaaa agatgccacg aaagcgcgga aagcatgacg agccccgccg cgacaataat
     2701 gaaaacggga aaaaataaag cgaacatgta ttgatgttaa agtgttgcaa atgatgtaaa
     2761 tgttataagt gcaagtaatg agaaaaatac ggaacattgc atcactacgg cattacaaca
     2821 tcatggcttc tatggtctca atccgtaagc atgaacctct gtcgccgccg gatcgatgtt
     2881 cgggtcggcg ggatacgtta ccgagaaatc gtatgttccg ttcggggcga cattatccaa
     2941 ttcggtttgc gatgcgccga tgatcgcgcc tgacgcatct ttgaaaagag cgatgacggt
     3001 caccgtatcg aaagcactcg cgtcgttgtt cgtaagcgtg ccgctcgtgc caatcatgtc
     3061 tgatgatatc gttcccgttt gcacgttttc aaggtcgaac tgcggcgccg taccggtatc
     3121 ggaactcttc gcccacacgg gattccggat gacgagcgcg acattactta ccgaggacgt
     3181 caccgcttcg ttcggcgcaa cgagatattc ctcttcaccg gcatagatga acgattgtcc
     3241 ggcaatcgac tgaacgaccg tgcccgacgc atcggaaaga tcgaaacggt aatcgaaatt
     3301 tcgcgccgtg aaatcggtgt ttgtgttcgc aatgccggca aggaacgtca cgcgccccgg
     3361 gctggtcgtg aatgtcatga cggcgccgag gacggtgatc ggctggagag ttgccggcgt
     3421 gcatactttc gcgcacggtc cgccgcaatc gaccccttct tcgccctgat tctggatatg
     3481 atcgaagcag gtcggcgcgg gctttaaata aaagaaataa atccatgcgc cgatgccgcc
     3541 caaaacacaa agatacaatg cgccgtaaac gatctgtttt gcgatgcggg acataacgct
     3601 attgtaaaac aattcagtgc tatgcgaaag cgcctcattt cagaaaatta ttttataata
     3661 gaggacgtac gcgcttgcgc cgctgcggtt tgaagcattt tcttatgatt tttttcaaat
     3721 gcgcacgtcc tgattaaata tttaaactca tgaaactttt tgtatcattt tcccgaatcg
     3781 ggaaacaatg cgaccagctt cgaca
//
LOCUS       DAIDDT010000065         3304 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_65_length_3304_cov_5.254726, whole genome shotgun sequence.
ACCESSION   DAIDDT010000065 DAIDDT010000000
VERSION     DAIDDT010000065.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65_length_3304_cov_5.254726"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          863 a          722 c          938 g          781 t
ORIGIN      
        1 tatcgctact gattaagaat atattaaata aaagcgcccg taaacggacg gattctttgg
       61 tgtcatgtcc gaaaaacaaa aaatgaataa aaaacaaacc aacaaaaaga aaacggtggt
      121 cgcggtctcc ggcgggttcg atcccatcca tatcggccat gtgcggatgt tcggccgtgc
      181 gcgggcgttg ggggatgaac tggtggtgat tttgaataac gataactggc tccgccaaaa
      241 gaaagggcat attttcatgc cggaccgcga acggaaagag gtgatcgaag cgttgcgcgg
      301 cgtcgaccgg gttgtactca caaaacacga ggcgggtgcg tccgacatga gcgtgtgccg
      361 cgaattggcg gagatcaaac cggatatttt cgcgaacggc ggcgaccgaa aattgggcaa
      421 catccccgaa gtgccggtct gcaaggcgat cggctgcaaa atgattttca atatcggcgc
      481 cggcgggaaa atacagtcca gctcgtggct tttggaagat tacattaaaa aagcggcgac
      541 gaagaagcgg acaaaaaaga aatgagacgt gtccgcgcgc gttgtgtttc aataaaaata
      601 tttttattga atgccgtcgt ccaaagggtt tacttgagcg agcggattac ttcttttatc
      661 ccggatttac gaatatcgat tttatcgttt taaggataat ggcgaagtcg aggatcagcg
      721 accggttctt gagataatac acgtcgtatt gcagtttttg cgcgacatcg tccagcgtgc
      781 ggtcggcgcg gtggttgacc tgcgcccatc cggtcacgcc gggtttcacg agaagccgca
      841 tttcgtaata aggaattttt tctttaaggg ttgcgacgat ttcggggcgc tccggccggg
      901 gtccaacgaa cgaaagctct ccctttacga tattcaagag ctgagggagc tcgtcaagat
      961 gcgacgcacg gaggaatttt ccgaaaggcg tcgcgcgcgc gtcgtttttc gcggcccatt
     1021 ggacgcccgc gcgttcggcg tcaacgcgca tagtcctgaa cttatagagc gtgaaggggc
     1081 ggctgttgtg tcccacgcgg acttggcggt aaatgacggg tcccggggac gtcaatttta
     1141 tgataatggc gatgagtatt tcaaaaggaa ggaggattat tccgacggca atcgcaaaaa
     1201 taaattcgcc ggcgtttttc agggggtcgt aaaaccgcga tgtccgttct atgttctcga
     1261 aaaaccatgc ttcctcgatt ccgacgagag ggattttatg cataacaatc tcgtaaaagt
     1321 cggcgatatc aatcacgaga acgccctccc cgaaaagccg gtagagtatt ccggcgagcc
     1381 ggttttcgcg tttcaggtgc cgcggtacca cgatgatatt cgccgctttt tcccgcgccg
     1441 cttcttccag taattcgggg cggctgttta tggaatcttc ttttatggaa agcgcgatcg
     1501 cataaccgag ctggcggttt tcccgtataa ccttctcgac ctcctccgcc gcggaaccgt
     1561 cgctgacgaa aaggacgtta ttgagggctt catgggatgc cgccgtacgg ttgaatgtcc
     1621 gccgccatat agtttctatg attatgaaga cgacgaggaa gataaaaagg tttgtcttgg
     1681 gcgcgatgcc gaaggcggga atcagataaa aaagaagcat tgcgaccacg gcgccgataa
     1741 tggtcgcgag cgcgagcgtt tttgtaaaat caagattatt gcgcaggcga cggagatcgt
     1801 aaagccccgc gatgtagaat atgataagcc acggaacgaa aatgatggtg aacggaacgg
     1861 catgggcgtc tatgaattgg cggtaaaagc cgttgccgta acggatgaaa agcgtggtga
     1921 aaagcgccgc gtagagcgcg acaatatcgc ccaagaaaag caaagttatt ttaagggacg
     1981 cccctttttt catgcgttca ttttaacata gaaaaagcgc tatttggaat gcgcgcctct
     2041 ttttatcatt attcgacttc aatgtatcgt tttttgttgt agtttttcaa tctttcggtt
     2101 cgtcttattt atctttataa agacgggaag catgggtcgt ccgtccgtct tcacggacga
     2161 tgacagaggg agcaggacga tggatggcag aagggaaact tttgaatagt tttattaaaa
     2221 tttgacacgc atggggaggt cgaatataat ttctaatatg gaaaaaaatc aacatcaaat
     2281 aaccgaagag gaagtacagg ttttcaataa aaaagaaacg ctggccggaa aaagcgaaaa
     2341 gaaaagagat tactttttgc cggcaagcat cgtcgttgcg ggagtcctta tcggcggcgc
     2401 ggtcgtgttt gcgctccttt ataaaggagg atcgtccgcg ggcaacggag cggcgcagaa
     2461 cgggaataac gccaacggtc aggccgcttc cacgactgac gttatgaacg tggggggccg
     2521 cgatatcgtc ctcggtaatg cgaatgcgcc ggttacgatc gtcgaataca gtgattatca
     2581 gtgcccgtat tgtggaaaat tttttgcgga aacacagccg cttatcgtta agaattatgt
     2641 tgacaccggg aaagtaaggc ttgttttcag gaatttcccg tttttggggc ctgaatcggt
     2701 tgcggcagcc gaagcggcgg aatgtgcaat agaccagaac cagggtgcgg cctatcatga
     2761 cgcgctctat agggcgaaat atgcggaaga agcgggtggc gggagcgaga acgacggcag
     2821 tatgaacagc gcattcttca taaaactcgc gcagcagctc aatctcgata ttccgacctt
     2881 tacgacgtgt atcaacagca ataaatatgc gaacgaggtg aaacaggaag aggcgcgcgg
     2941 tgtcgctgcg ggcgtgaatt caacgccgac attcttcgtg aacggccagc aggttctggg
     3001 agcccagccc tatgcgacat tccaggcggg tattgatgcc ctgttaaaag gacaataacc
     3061 aaggacaata aaatgatccg caattggacg gagcttgtaa tcgggctttg gatcatggtt
     3121 tccccgtggc tgcttggatt ttcaagcatc ggcatcatga agtggagtaa tgtgctgtgc
     3181 ggactcgtgt tggttctcat gaacgcgtgg gcgcttttcg ggaaagaacc tatggatcat
     3241 gagggagagg ggaaataaaa aattttattt gttcgtcggc cccccccgaa gagaagtgtc
     3301 catg
//
LOCUS       DAIDDT010000066         3280 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_66_length_3280_cov_6.168280, whole genome shotgun sequence.
ACCESSION   DAIDDT010000066 DAIDDT010000000
VERSION     DAIDDT010000066.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66_length_3280_cov_6.168280"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          716 a          975 c          845 g          744 t
ORIGIN      
        1 aatgaaaata acgctcgttg ccaggccgca aaaaaacatt gcatttgttt tcttcctttt
       61 tgaaaatgac cggatcgacg cccagccgct cttccttttc cttgaaaaac cggaacagga
      121 ctatcttcgc ggcgcggaaa aaagtcttgc gcttaaagaa agggaaagca ggacgctcat
      181 catgcccggc agcggtcgga aaatcatcac gataggcctc ggaaaagccg ccgacgcttc
      241 ggtcaaaaaa atgggaatcc cgcaccggat ggcggtgcag gcggcgagag cggaaaagat
      301 agaacgcttt gcctattggg cggcggcgca aaaaacatcc acggcgaccc gggaaacgta
      361 tgaggttgcc gcgtcaaatg cgatcatggc gaactttgaa ttcgtgaaat ataaaacgcc
      421 gacgccggaa ggcggcgcgt tcgtcaagga gatcgaagcg gtcgccgcga aattaccgca
      481 gcatgatatt acggaaggga tccgccgcgg catcgtgatc ggcgaggaga cgaatgcctg
      541 ccgtgaactt tccaacacgc ccggcggcga catgacgccc gcgatcctcg cggatcacgc
      601 cgtcaaagaa gccgccaagg ccggcatcaa agctaccgtg ctcgacggaa agaaaatgaa
      661 gacgctcggc atggacggca ttttgggcgt aggcagggga tcggacgcgc cgccgcggtt
      721 cattattctc gaatacatga aaggagcgaa aaacgaaaaa ccgacggtgc tggtcggcaa
      781 gggcgtgacg ttcgataccg gcgggctcaa cctcaaaccg ggcaaccata tttatgagat
      841 gcatatggac atgtccggcg gcgcggccgt catccatgcc gttatcgccg cggcgcggct
      901 gaaactcaaa aagaatatcg tggcgctcgt acccgcggcg gaaaatatgc cgtcgggatc
      961 gagctatcat cctggcgaca tactgcgcac tatgaacggc ataacgatcg aagtgctcaa
     1021 caccgacgcc gagggccgcg tgatcatggc cgacgctctg gaatattcaa aaaaatacga
     1081 ccccgcattg gtgatagacg cggcgacgct gaccggcgcc gcggcggtcg ctttgggaca
     1141 gcagtattcg gggcttttca cgaccgataa gaaactggaa gaaagattca gggagctcgg
     1201 tgaagcggtg aacgatccgg tgtggccgtt gccgctcagt gccgagtacg aaaaagacat
     1261 caaaggaaca ttcggcgact gggcgaacgc cggcaagccc gacacggcag gcgcctcaac
     1321 cgcagccgta ttcctctggc aattcataaa agggtatccg tgggtgcatc tcgacatcgc
     1381 gccgcgcatg acggcgggcg aaggcgatta tctcgcaaag ggcgcactcg gaacgccggt
     1441 gcggctgctt gtacgctttc ttgaaaatta acggcgggcc cccgccctcc ttcaaacaca
     1501 aaccccggcg cacagccggg atttgtgttt attttttacg gacaaaccgc aaaaaatgaa
     1561 tatctatctt tcaaaacgga ttatcatcat ttctaataat cctctcctcc catcatgccg
     1621 ccgggcatac cgccgcccat cggcgatgat ttcttttcgg ggatgttcgt tatcgcacag
     1681 ccgaccgtga gataattcgc ggaaaccgaa agcgcgttcg tgaacgccat tttcacgacc
     1741 ttcagcggat cgatgatacc ggcctctttc agatcgccca tcttgttcgt gagcgcgtta
     1801 aatcctttcc acggctcgtt cttcctgcga cgcaattcgt cgatcacgcg atccgccgat
     1861 tcgccgctgt tcgcgacgat ggcgcggatc ggcgcttcaa gcgcgcgggt cacgatccgc
     1921 gaaaccgcgc tctctaccgt tttcccgtcg ccttctttta atgaaagctc catgctcacg
     1981 ttgaaaagcg cgatgccgcc gcccggcacg ataccttcct ccatcgcggc gcgggtcgcc
     2041 gcgaccgcat cctccacgcg ctgcttcaat tccttctgcg cggattcggt cggcgcgccg
     2101 actttgatga ccgcgacgcc tccggcaagc ttgccgagac gctcctgcat tttttcttta
     2161 tcgaaatccg attcggtttt tttgatctgc gccttgatgg cggcgatgcg gctttcaatg
     2221 tccttcttct cgcctttgcc gtcaacaatc gtcgtcgcgt ccttattgga aataacgcga
     2281 tgcgcgcggc caagatcgct caagtcggta ttttcaagct tcttaccgag atcatccgaa
     2341 atgaactgcg cacccgtcac aattgcaata tcctgcagca tctccttccg gcggtcgccg
     2401 aaaccgggcg ctttcaccgc aagcacgttg aaaacaccgc ggagtttgtt caacactaat
     2461 gtcgtcaatg cttcgccgtc cacttcttcg gcgacgatca aaagctcttt tttcccgctc
     2521 gccacgattt tctcaagcag cgccagaata tcctggatgg acgatatttt cttgtccgtc
     2581 accagaatat acggatcgtc gagcaccgcc tccatctttt cctggtcagt caccatatat
     2641 tgcgaaacat aaccgcggtc gaactgcata ccctccacga tctcgtgcgc attgccgatc
     2701 gtgttcgaat cctcgacggc gaccacgcct tccttgccga tcttttccat caccgccgcg
     2761 atgagcccgc caatctcctc gtccttcgcg gacaaggtcg cgacctcttt tatctttttg
     2821 ttgtcattga taagctcgcg ctggttttcg agcgccttga tgatctggtg gctcgttttc
     2881 ttcagctcct ccgcaagctg gatcacgttg aacccttttt cgcggatcac tttctcgcct
     2941 tcgtcgatca tcgcgtgcgc caaaaccgtc gcggtcgtcg ttccgtctcc cacgccgtcg
     3001 ttcgttttgt cggctgcctg cttgatgaat tccgcgccga cgttttccca tttatcctca
     3061 agctcgattt ctttcgccac cgtcacgcca tcgaacgtca cctgcggcgc gccatagccc
     3121 ttttcgatga caaccgcgcg gccgcgcgga ccgagcgtca tgatcaccgc ttcggcgagc
     3181 ttatcgattc ccttttttat cgacacccgc gcgtcttcgt tgaattttat ttgttttgcc
     3241 atggtaatag atatttgtaa tttaaaaact tgaaatgaat
//
LOCUS       DAIDDT010000067         3265 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_67_length_3265_cov_6.204517, whole genome shotgun sequence.
ACCESSION   DAIDDT010000067 DAIDDT010000000
VERSION     DAIDDT010000067.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67_length_3265_cov_6.204517"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          826 a          918 c          743 g          778 t
ORIGIN      
        1 attctaagtt ctgatttcta tattctattt acgcctgttt attatgaacc gaaaaataat
       61 actgcgcgaa cgccaggaac acgaaaagac aaaggtacat cggcagcacg tcgaagatcg
      121 cgatgccctg gacaaggtaa gcgataggaa ccgccaagat aagtccgcgc tcaaacaaag
      181 cgacggtatt tttcgcatgt ccttcgcggt gatgcgccga agcgccttgc tccgggcccg
      241 tcaaacgggc tttttgtttg cggaaaaatt gccaataaaa cgttacgaat atagaaaggt
      301 atgccagcaa accgagtatc cccgtttccg aaagataatc gaagaaaacg ctgtgtgcgc
      361 ggtcaaacca cgtttctgac gattgtccgg gaacaaaaaa cttcggattg aaatgtttat
      421 cgtaaacggc cgtgaaattc tccggccccc agccaaggat cggccgttcc aagaatccct
      481 gccacgcctc gttccaaacc caaaaacgcg tctgcaccgt cgcgtcggaa aggctgatgg
      541 ttaaaaatct tcctcccggc agatttttca cgaagggaat gttccgtgcg agataagcgg
      601 cgccgcccaa aacaataagt atcgcaagaa taatggccga ccatttttta agcgcggcat
      661 tcttggaaaa aacaaaataa ataagtaccg cgaaaacgcc ggctcccaaa ccgataaatg
      721 cgccgcgcgt ctcgctcaga acaaaaaaga aaagaaaaat ggcgataaga atgccgtaca
      781 tccatgcgcg cgctttcgcc ggcatcttgg aaacatcgcc cttcgttatc caaagatatg
      841 cggcgaagaa catcgaaaaa ataaggtacg gcgcgacata agcaggattg ccaagcgacc
      901 cctgaaaacg gccgtcaacg agcgtatgcc agaatcccac aggggtggcg ccgccggagt
      961 agggaccgat aaatccctgc gcgccgaaat ttccgaatag tccgtaaacg atcatcgccg
     1021 tagcggcgac aagggaaaat ttgaacaaat tccgccactc gccttctttt ttgaaaagaa
     1081 gggaaagcag gatgaaaaag ccgtaataat gaagcatctg gaatgctcct tcgccgcgtt
     1141 caaaatttga ccaaaacgcc gcatgggggt cgtaggcgaa gatcgacgcc agcgtcacca
     1201 taaggacgaa cgcggacacc gcgatgaaaa gcgggctttt tacgagtgtc gcaaccatcc
     1261 gtttcgcttc gccgtctttc gcttcaaacg cccaccataa tatgaacgcg ataaggccga
     1321 gctcgaccgc gaacctgaaa aaccaatctt ttccgccgat aaacgggaaa aaaatggaat
     1381 ccatcacgat aaccaccgca aaaacagcgg cataacaata ccaccgcgcg atcctttcga
     1441 aggattgccc catcatcttc gccgcaatac cgagcaacgg aagtcccgcg acaaccgcaa
     1501 gcaccaaagg atggctaaaa agagtatcca taatattatg aagttaatta tatattagtt
     1561 caaaaaataa atacaatcgc tttgtcttct ctcccctccg tcgtcctcgg cttggctgag
     1621 gatccatgct ttaagaattt aaaagaaaga ttggaagcat agatccctgc cttcgcgagg
     1681 gcgacatgcc atgagccttt ttcttttacc gcgcgtattc caccgcccgc atctcgcgga
     1741 tgacgttcac ttttatctca ccgggatatt ttaactcgga ttgtatcttg tccgcaatct
     1801 cgcgcgcaag ctcgagtgcg cggaagtcgt cgatcttttc cggcgtgacg aacacgcgca
     1861 actcgcggcc ggcggaaatg gcataggcat ttttcacgcc gggaaattcc gcggcgatct
     1921 tttcgaggtc gccgagccgt tttatatagt tctcgatcgt gccgcgccgc gcacccggac
     1981 gggcggcgga aagaacgtcg gcggccgtca cgacatacgc ttcgggccgc gcatacggat
     2041 aatcgtcgtg atgcgattgc atcgcttcga caaccgcatc gctcacatta tattttttta
     2101 aaatctttat cccgagttcc acgtgcgttc cttcaacctc atgatcgatc gctttgccga
     2161 tatcgtgtaa aagcgcgccg cgcttcgcga catcggcgtt caacccgagt tcgcgcgcca
     2221 tcatttcact caaatacgcc acttctatcg aatgggtcag gacgttctgc ccgaagctcg
     2281 tcctgaaatg cagccgcccc aaaagctgga gaagttcttt ggggagatcg taaatactga
     2341 gatcctgcgc ggtggcatcc ccgatttcct gcatccgctt ggaaagttcg cctctcgctt
     2401 cctccacttt ctcctctatt tttgcgggct gaagccgtcc gtctttcata aggttttcaa
     2461 gcgcgagccg cgccacctcg cggcgatacg gatcaaatga cgatataatc acgccgtcag
     2521 gcgcttcgtc gatgataaat tccacgcccg tcgcgcgttc aagcgcgcgg atatttcttc
     2581 cctcgcggcc gatgattttc cccttcagat cgtcattcgc cagcggaaaa atagtcgtcg
     2641 ttatttcgga aacatgcgaa cgggcgtacc gctgcaatgc ggtcgtaatg atatcgccgg
     2701 ccttcttttc cacttcttcg cggttttcct tttctatctt ctggataacc tgagcaagat
     2761 cgttcgccct gttttctttt acgtggcgaa gcacttcttc ccgcgcgtcc gccgccgaca
     2821 gtccgctgat cttttcaatc cttgattgaa tgtccgcgtt ctttttttca agctcttcct
     2881 ctcctgcacg cacctgctct tcccgcgcat gcattgattc accttgctcc aaaagcgcgc
     2941 cctctttctt atcaaataat tcttccctgc tcaaaagacg cgactcgagc gcttcaattt
     3001 cttttctcct ttcgcgttcc tcgttcttcg cgtcaatcaa aagcgaagcg gccttttctt
     3061 tcgcttcgat gattatttct tttgcctgcg cctcggcgga cgaaactttc cctttaagct
     3121 gcgcctcgag cgaaccgagt tttttagtcg cccataagcg gcgaatgaag tatcccgcga
     3181 caaagccggc gcaaagagcc gctcccgctg ccaacagcaa tagagaatcc ataatgtttt
     3241 attttaaatt tattcatacc aataa
//
LOCUS       DAIDDT010000068         2873 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_68_length_2873_cov_5.415951, whole genome shotgun sequence.
ACCESSION   DAIDDT010000068 DAIDDT010000000
VERSION     DAIDDT010000068.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68_length_2873_cov_5.415951"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          725 a          751 c          699 g          698 t
ORIGIN      
        1 gtataatatc catatatgaa aaaaatcctc gcttatcttc tcgtcgcggt catcattatc
       61 ggcgtcatcg gggcggttat ctattttttg acgcgcggaa acagccagcc gggaaaccag
      121 acgggacagg cgggatcgct tccttcttct acccttaacg gcgggacggc gggacaggcc
      181 gcgggcggga cgcttcccgc cggatctttt gcggggagcg gcaccccttt cacagcaacc
      241 ggtgtttcca atattatagg aaccgtgccg gcgcttgatt attttgtcga caaccggaac
      301 aatgcggtcg taatcgaacc cgacgggacg gtcgcgggca tcgcaaacaa caacaccgat
      361 atcataagct cttcaaaaat ccagaatatc ataagcgcgg cgttctcata cgacggcgca
      421 aaactgctcg tcagtttcgg cgacgcgcgg gatccccaga ccagcgtatt tgatataaat
      481 acaaaatcgt ggatggcgct gcccacggga atggtgtcgc cccggtggtc gccggcagac
      541 gatcggatcg cgtatctccg cagtaatgcc aacggcaccg caacactcgc aacccttgac
      601 gcgtcaaaaa cgaaaaacaa tattacagcc gtcgcaacgc ttcatgtcca ggatatttcg
      661 ctcttatggc cgtcgaaaaa tagaatttta ttctacgacc gcccgagcat ttatgccgac
      721 gggtcggtgt ggtcattcga tctccagaaa aaaagcttga cgcccgttat catcggacaa
      781 cgcggactgg aaacaatatg gagtaatacc acgaccacga tggggcttgc gtttatcggg
      841 aacgcctcac aatacggcgg ccagttacag ctcgttgacg gatttggaaa cacaatagaa
      901 cagctaagat tccttacgtt gccgcccaaa tgcctcttca gtatgttttc cgcttcgcaa
      961 acggcctccg ggacggcgca ggccgcgtcc gccggaaata cctcgacaac ggcggcatcg
     1021 tcaaccgtaa atcctcctta tctcgcgtta tactgcggcg tgccgcggga ccagaacgcg
     1081 ctctcgtcca cacgattgcc cgacgattac gaaatggggt cgctgttcac ctccgacaat
     1141 atctatcgca tcaatacggc gagcggcaac attgatacgc tcttcgacga tcaaagccag
     1201 aacgtggacg tttcggatat gaagattttc aataacgctc ttttctttat caaccgctac
     1261 gaccaaaaaa tctattcgat agcgctataa agacggaatt ccccgcggta aacgcaaaaa
     1321 atccatgcgc gcgcatggat tttttgccga tgatcaatac cttaatcacc gatcgccgcc
     1381 tcgttttttt ctgtcttttt ctcaaaatta atcggcttga taatgacata ccttcttttt
     1441 cccgctccgg cgctttccgt cgcgatgtcg ggatgcaccg ccagcgcaac gtgcaccaac
     1501 cgcctttcat atgaattcat tgcgggcagg aatatctctt ctttggtggc gagcgccttt
     1561 ttcgccgccg ccttggcgag ctcagtgata aggttttccc gctcctgccg gtaatggtta
     1621 atatccaaaa aaatcgcctt gttctggttt ttgcgcgcca gcatctgcag gatatgattg
     1681 aaagcgtcaa caatcacggg aagatccttt ttaaacagtt cggcgtcctc ataaataaaa
     1741 agcgaggcgc ggccttcttc cggcagcact tcaagccggt aatcccggaa tcccatgcgg
     1801 tcaattattt ccttaataag cggttcccat gtatccattg gatttttata atgattttag
     1861 acttaatcac tatttcattt ttcggagcaa aaagcaagac ctccggcgaa ggaggcgaaa
     1921 cgcgccgcac tctatatctt cctcttctgt ttattgtccg tatttttcct tcagcttctt
     1981 attgataatc aactgctgtg ccaccgaaaa aacggacgag acgagccaat aaaggccgac
     2041 ggcggccggg aaaccgtaaa aaataacaat ggtcataata gggcctatga acgacatctg
     2101 gctggctatt ttttgcgcct gcgacggcgc ttccccgggc atccggtaga tagcgaggcg
     2161 cgcctggaag tattgcgcaa tagccgccaa aacgacaagg gtcaggtttt tgtcggcaag
     2221 gttaatcagc cccagaaacg tcgcatggac aacacccggc gcggcgacaa aagaataaag
     2281 ctggttcaaa gtgcccgccc cgaggctcga ccgcacgatc tggtaaagcg cgataaggat
     2341 gggaagctgg atgataagca gaagaaggct ggaaaacgga ttgacgccgt gttctttata
     2401 aagatccata agcgccttgc tttgtttttc cttgtcgtct ttgtgcaatt cctgaatttt
     2461 ttttatcttc ggctggaggc gctgcattac catctgctga tgggaccctt tatgaaaaag
     2521 cgggaagagg atgatacgca cgagcaatgt ggtgaggatg attgcgaggc cgaagtcgcg
     2581 gaacgcaatg gtatcgtaaa aaaacacgag cgcgttgaga atcggctggt aaaaaataat
     2641 attatagaga taagccatga tagacagtat acctgtttgc cgtatttatt gcatggcggc
     2701 gcgcagaaac gcttctttga attttttcgc cgtgcatttt tttatgcctc cggaaaaaat
     2761 aatcagaaaa tcattgccgg cgccgggcac gagcatgaaa tccgcttttg cccgccgctt
     2821 ccaaaaattc ctgcgtgccg cgctttttac gacggcagcg cttattatga ttc
//
LOCUS       DAIDDT010000069         2847 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_69_length_2847_cov_6.885199, whole genome shotgun sequence.
ACCESSION   DAIDDT010000069 DAIDDT010000000
VERSION     DAIDDT010000069.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69_length_2847_cov_6.885199"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          612 a          810 c          679 g          746 t
ORIGIN      
        1 cgacatccac caccggcccg atgattttta ttatttttcc tgttttcata ataattataa
       61 taagttgaat tccttctgtt attcgcttat tttctgtcat cgtccgcctc cgctgacgat
      121 gacagaaaag aaaattggta ttgcattatg ccgccgcttc cgcgcccgac gtcacttcga
      181 taatcgcgtt cgtgatcgcc gcttggcggg atttattata tacgacgttc agtttttccg
      241 aaaggtcttc ggcgttctcc gaagcgttct tcatcgccac gcgccgcgcc gaatgttccg
      301 acgcgttcgc ctcgagtata agatggtaca gttgtatttt taaaagcttg ggagcaagct
      361 cttccatgac ttcgttcgcg gacggctcga taagatagtc cccgttctgc cgccccgcga
      421 gggattcgat atcgatatag tgcgaataac gccccgcccg cggaacggtt tcctcgatac
      481 tttcttttat tttcgcgaac gtgaccggaa agagctggcg catgatggga tcctgacgca
      541 aagcgctcac gaaacccgtt gaaaacaaga ttacttcgtc gaaatcctcc cgcaggtagc
      601 ccttgatcaa aaaatcggcg atcggttcca cttcttccat ttttgaagca tcgcccatgt
      661 ggcggaacga ctgcgcaaga gcaagattcc tgcgttcgca ataggcgcgc gccttctggc
      721 cgacggcgat aaaggaatat tttttttcgt acacgtcgat agcgtgcgcg cggatgaaac
      781 tttcaaattt gcggatgacc gcgctgttga acgatccggc aagaccctta tccgaagcga
      841 taagcatgaa cgctgtcttc ctgaccggac gcccgcgtaa aagtttcggc agtggcgaat
      901 atacctgcat ggaaagaatc cccaaaatgc cgagcgcggt atacgaatac ggacgcgaat
      961 tgagcgcgta ttcctggctt ttgcgcattt tcgtaacctc gacaagctcc atcgctttcg
     1021 tgatcgtccc gatattctgc gcccccttca ggcgccgctt gagattttgg agagattcca
     1081 ttggataata atcaaaagtt gacaatgaaa agtggcgtga agtctttagc gatgagggaa
     1141 atttctctcc ttcgccgttc tcgaccctcg attaggtcaa gagcatgctc tggtcgagga
     1201 tccatacttc cttcttactt cctactttct gccttccgtt ttctacgcct aaagcatgga
     1261 tcctcgcctc cgcaaagacg acagaatggt tactttccca agaattttcc tatcacttct
     1321 tttaatttgg tttcgacttg ttccgtgaat tcgccggtct cgcggatcgg cttcaaaaca
     1381 tcggcttcgt gaaactctcc gaggtattcg agtaagcggt tttcggtctc ttttatgttt
     1441 tccacggcga tatgctcgaa ataaccattg agcaccgcgt aaaaaacgca cacctgtttc
     1501 tcgaacggca acggcgcaag gtcttcctgc ttcagaatct cgttgatctt ctgcccctgg
     1561 aggatctttt tcttcgtcgc atcatccaca tccgtcgcga actgcacgaa tgcctgcaat
     1621 tcgcggaact gcgcgagcga gagccgcaat tttccggcga cttttttcat cgccttcgtc
     1681 tgcgcggccg aaccgacgcg tgaaacggaa atgccgacat tcagcgctgg ccgcaatccc
     1741 tggttgaaaa gatccgattc gagataaatc tgcccgtcgg ttatggaaat gatgttcgtc
     1801 ggaacgtacg ccgtcacgtc gccgagctgg gtctccacga tcggcaatgc cgtcagcgac
     1861 ccgccgcctt tttcttttga aagtctcgcg gcgcgttcca ataaacggga atggagataa
     1921 aaaatatcgc ccggatacgc ttcgcggccc ggcggccggc gcagaagcag cgagatctgc
     1981 cggtacgccc acgcatgttt cgaaaggtcg tcgtaaacaa tcagcgcgtc ctgcccttta
     2041 tcgcggaaat attcccctat cgcgcagccc gcatacggcg cgagatacca cattgccgcg
     2101 ggcgacgatg ccggcgccga aacgataata ctgtattcga acgcgccgaa ttttttcagg
     2161 gtctccgcgg tcttcacgac cttggattcc ttctggccga tggcgacata aatgcagacc
     2221 ggcttctttt ttccggtttc ctgcgcctgg ttgatgatag catcgatcgc gatcgccgtt
     2281 ttcccgacct gccggtcgcc gatgataagt tcgcgctgtc cgcggccgat gggaatgatg
     2341 gcatcgatcg ccttcagccc cgtatgcagc ggcgtcgtga ccgattcacg ctccagaacc
     2401 gacggcgcgc gcgcttcaag cggattgaac tgcggttttg ttttcgtgaa aatcggccct
     2461 ttgccgtcca aaggatttcc cagcggatca acgacgcggc cgataagttc ctcgccgacc
     2521 ggaatggaca agacatcgcc ggtacgtttt acttcattgc cgacctcgac cgcaagaaaa
     2581 tcgtcgagta caagcgcgcc gatcgattct tcttcaagat tcagcgccag cgccgtacgc
     2641 tcgccgcgcg gcgtttgtat ttttaaaatc tcctgactca cggcgttccg caaaccgacg
     2701 atcttcacga tgccgtcgcc aacctcgatc acgcgcccga attcctccca cttcggatcg
     2761 tccttgaaac cctctatctc ttttttcagt ttttctatga tttcgtaact cataatttta
     2821 atatttcatc aactatccct tctgtca
//
LOCUS       DAIDDT010000070         2740 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_70_length_2740_cov_5.209163, whole genome shotgun sequence.
ACCESSION   DAIDDT010000070 DAIDDT010000000
VERSION     DAIDDT010000070.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70_length_2740_cov_5.209163"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    2291..2307
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          626 a          769 c          700 g          628 t
ORIGIN      
        1 tgaaattaat tggataaaaa aaattaggac ttacgcgttt gaataaaaat atttaaaaaa
       61 cgtcggatgt ttcaaaccgc aatggacagg acccattact tcattatttt cctgcaattt
      121 tcatcgccgt aatcggacta cggcttccaa aatcctcgga aaatgcctcg tacccggcac
      181 acgtgcgtac gtcctataat actaatactt gcttcattct gtcatcgtcc tgccgaacgg
      241 gacgatgaca gaggggaacg cagtggcggc ataagaatgc cgcttttcac ttttagtttt
      301 tagtttttat cctgttttca taataccaat tcaaaacgtc tttcgccacc ggcacggcgt
      361 tcaagcttcc ttgtttggaa ttttctataa ggacaagtat ggctatcttc gggttgtcgc
      421 acggggcgta acccacgaaa aacgcgtcct cctccttatt gtcttttatc tgtgcgctgc
      481 cggttttcgc gcaggatgga aaaggcaggt catccatggt ataggcggtt ccctgaggcg
      541 acgttacggt ttcgcgcatt ccctgctgga cctgttttat ttcgggaagt agcgaggtga
      601 gatcctcgtt aaccgagggc gttgacgacg cgttcaagaa cggccggtaa atcgttccgc
      661 cgttcgcgat cgctccgata taatccaaaa gctggatcgg cgtcaaaagc aggtcgccct
      721 gcccgatgga aacgttatac gtgtcgccca aaagccacgg cgtccccatt ttcttctgtt
      781 tccagtcggg tgtcggaaga aatccggatg cctcgccggg caaatcaatc cccgtcggct
      841 ttcccagccc gaatttttcc caccagtcat gaaggcggcc gataccgagc ccgcttatgc
      901 cgtaatcgct cggatcgttg atgagcggcg tggttacggg cggcgaaccg cctcccacaa
      961 tataaaaata aacgtcggac gattgcgcaa gcgcggcggc gagattgacg cttccctgat
     1021 acctccagtc gagatagcgg gtcggcgtgc tcgaattata cggatccggc accataagat
     1081 atccgggcga aaatattgcg cgtgtggaat cgatcacgcc ctcatgcagc gccgccacgg
     1141 catcaagcgg ttttatcgtt gagcccggat tataaagacc gctcacggcg cgatcgaaca
     1201 gcggtttgtc gggcgaagac aaaagacttt gtatcgcggc ggaattcccc gagcccgaaa
     1261 aaacgttgtt atcgaaaccg ggaagatcca cgagcgacag cacggcgccc gtttgcggat
     1321 ccatggcgat gccgaggccg acctgccgcc ccagcctcgc gaggtcgctt tgcatctgcg
     1381 aatagaaata cgactgcaga ccgccgtcta tcgtaagctg cagcggatgc ccgatcacgg
     1441 gcggcgattt catttctttc gtcaggacat ttccctgcgc attcctgaaa gaaacatcca
     1501 cgcccggcgt tccccgcaac gtgctgtcat aaaacgcttc aagaccggct ttcccgataa
     1561 aatcattgcc ggaaagcgcc ggattatttt taatatcgtc cccgttcacg cggctcacat
     1621 atccgagcac cgtggaaaat gcaggacctg cgggataatc acgctcgaaa tcactcttga
     1681 cggtcagcgt cggaaggttg agcgcttcaa gattcaccag ctggttcggg tcgacattct
     1741 cgcttaaaac gatcggcgcc gcgaaatcct gcgcgcttcc ctggttgatg agcgaccaca
     1801 cgccgtccgg cgcaatgccg aaaatattct ggatcgccgc gagcgtgctc gtctgcaaat
     1861 tctcattttg gagaaattca cgcgggtcga gcatcgccgc aaacgccgcc ttgttgtcgg
     1921 caagaacatc cccttcgcgg tcgtagatga tcccgcgcgg cgcgggcgtt tccttcaatt
     1981 gagcgacatt cgcctgcgcc ctcgccgtgt aataagcgcc gtccgcgcca agcgatatta
     2041 tttctaccgc aactgcgatc acaatcacgg cgatagcgat accgagatag agaaacggac
     2101 ggtctcccag cggaacttcg gccacgcccg catcgcggtt ccattcgtca ttcaacgatt
     2161 cttcaaaagc aatgattttc ttttgttttt tacgccttaa catgagttca gattagtgaa
     2221 acgggaatgg tttgtaaagc tgacgggggc ttgaaaacga aatgccggag cgatggcggc
     2281 gtataccgcc nnnnnnnnnn nnnnnnnaag aagtcgcggt tcccgccgcc ccgttcgttc
     2341 cggatgttcc cgcgggaacg ctgcccgctt gtgccgcaga agcggcgctt gccgccgctt
     2401 gcggattttc gatctcctgg acaatctggc tgtcgttccg aagcggcgcc gtaaagagta
     2461 cctgcgccgg gttaaaatag atatcaccgg tcggagccca tgcaaccgat ttgaacattg
     2521 ctatgccata ctgaggctgt ccgttctggc cagtgccttt cgtgacgtac atcgcgtcgg
     2581 caatgtacgg cttggggaac cagtacaatt taccgaagta aatatcgccg ctcgtcatat
     2641 aaaccgccgt ataacccgac gccgcggacg gattcgccga ttggcttccc gcaacctcga
     2701 cgccgatcaa aataccgata ccgacaagaa cgaccaacac
//
LOCUS       DAIDDT010000071         2448 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_71_length_2448_cov_4.308309, whole genome shotgun sequence.
ACCESSION   DAIDDT010000071 DAIDDT010000000
VERSION     DAIDDT010000071.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71_length_2448_cov_4.308309"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          675 a          541 c          668 g          564 t
ORIGIN      
        1 gttaatggac gacggaaaag aggggcgcga ggagttacct tgttctctat tctgacttct
       61 atattgagat atccacacgg cccttttcca tatataaaga agagatgata tacttttagg
      121 atatgaaaaa aattatcctt gaaaaagacg agggggttgc ggaggccgtt gatcggatat
      181 tgaacgaacc cgacaatgac gttacgctcg tggtccccaa tggttcggcg ctggggaaga
      241 cgatgcggaa tttcaatatt ctgaagcggg aagtcgaagc tgcggggcgg gacattacga
      301 tcgagtcggt tgatgagaac atcctggctt tcgcgcgcaa aagcggcata gcaggcagtc
      361 atcctctctg gacgggatcg ccgcacggcg gcttttcgga tattctctcg ggagaaaata
      421 acaatgacga cgatccgccg cctgcgcaca aagtaaatcc tctttcctca aagtcacgga
      481 atgacgcggt tctgaaaccg gaagtggatc gtaaaaggac gcctattaaa aagaaaatag
      541 ccggagaaac gccggtgaca atcgcggtgc aaagcgaaga cgaagaggaa gatgagaata
      601 cggaagaagc taccgaagat aagcatggcg atgccgataa acatcggtcc gtagcggaag
      661 agaaaaaagc agtgattgag aaaaagagcg acgaaccgga aaagaaaaat ttttccggcg
      721 gaaacaattt ttttaaagag acggcgatac ccgacgcgca tggcgatgaa tcggaatcgc
      781 atcgcggcgg cgcgaaaaaa gttatcggtt atatcgtgct cggcgtcatg gtcatcgccg
      841 ccgtgttttt tgccgtgccg cagtttttcg ggagcgtcaa agtgacgatt gacttcaaaa
      901 aaacgccgtg gggatacagt catgctttca tggcggacaa atcggttgca acgtcgaccg
      961 taggcgtggc ggacaatatt attcccgcgc aagtatttat tgcaaccaat agcgccgtcc
     1021 agtctttcca cgcatcgggt atcgcgaacg tttcgctgaa agcaacgggt gttattacta
     1081 tttataatga ctacagtgcg gcgccgcagg tgcttgtcgc gacgacgcgc ttcgtaacgc
     1141 cggacggaaa gatattccgt ctggtaaatc gcgtgaccgt acccggggcg caggtggcgg
     1201 acgggaagat cgttccctcc tctctgaacg cgccgattat cgcggatcag gcgggtgccg
     1261 cgtataatgt cggtccggtg acgaaactta ctgttccggg ttttcagggt acggcgaaat
     1321 ataacggttt ttacggatcc atcgcaagct tgactgccgg aggtttcgtc ggcaagaagg
     1381 cggtgccgac cgcgagcgat atcgccgccg cggaagccaa cgtaaaaaat atcttacaat
     1441 cggcgacctc aagcggcggc gctttggcaa aaattcctga aaatttcgaa attcttgacg
     1501 gagcgacagg gacgacgatc acaaaactta ccgtgaatac aaacaccgac cagaacggca
     1561 atttcaacgt attcggagaa tcggtttttc aggcgattgg ttttgataaa acaaaactcg
     1621 aggcattttt gctcgcgcag gcggaaagcg gcaggacagt gagttccact tttaataatt
     1681 ctttaaacat acattacagc ggaacacagg ctgattttac gaaaggtatt atgagtttca
     1741 cgcttgatgc ttcgggaact attgaaccgg cgttttcggt tgataatttc cggagtgcca
     1801 ttcttggcaa aagcgtcgcc gacgcccgtt cgatgatcgc gtctcttccc gaacttgccg
     1861 acggcaccat cgccgcctgg ccgtcatggc tcgcgaatat cccgtccgat ccgggaaaag
     1921 tacggattac ggtgaattaa gaatattttt ttaagcaaaa cttcctcgcc gctcttgttc
     1981 cgtactatag tactaagtac ggaacaagag cggcaatgga cgggtgttcc gcgctatttg
     2041 acaataatag tctttctcgg gtatactctc attacgttta tggggttcaa ttatatcgta
     2101 agaatcgaaa tggagggcga acaagcgtgc cgtaacaaca aaaggatagc gccttttatt
     2161 ttgtagcatg gaaggagcga aagaagggac tgcttttgag gcgcttccga acctgatgtt
     2221 ccgggtcaag cttaaggact cgggagagac ggtgcttgca catatggccg gtaaaatgaa
     2281 gcttcataaa atacgggttc ttcccgggga tcgcgttctt atcgagatga gccccgacgg
     2341 gggacgggga agaatcgtga gaagattata aataatagaa tagagcatag agaggttaga
     2401 atatagaaaa aagaatccac attctctatt ccaagttcta acttttga
//
LOCUS       DAIDDT010000072         2371 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_72_length_2371_cov_6.010026, whole genome shotgun sequence.
ACCESSION   DAIDDT010000072 DAIDDT010000000
VERSION     DAIDDT010000072.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72_length_2371_cov_6.010026"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          593 a          564 c          407 g          807 t
ORIGIN      
        1 ataatatctt catccgttat tgatttatta tcatagtcat aagagctggg agtatgaaaa
       61 tgtaaatccc acctccccca ttcagaacct cttgaatatg gactattcat agaaatttat
      121 tgtttaattt aaatccaaaa atttccaatc cttcaaagct ttcggattat attcgtattt
      181 ttcattatcg ttatatctcc actggccatc agtggggata acaatgccac ccgataattt
      241 ctttcccttt ttcctttgtg cttttatgta ttttgctaat ccttccgctc gctctttcgc
      301 gtcctcggcg gtttgtccct ttttggtatc caataaccat acctctccgt ttttcaatcg
      361 gataataaaa tcgacataaa acgccgccat cgctccatgc ttgtctttgt aaggaacagc
      421 gaaataattt acctcgccat agccattttt aaaccaccat tcaatcttct tggccttatc
      481 aatataaccg ataaaatcgg cttctgtttc gctatcgaac aaggcctgct gattgtcctg
      541 cttaaacttt gaatagaaag gcagcatcac cgacttctta taggccttcg ctttatactt
      601 gctgttatag ctaagtatct tcggcacatt ccaaacgaga gtttccattt ctcgttcttc
      661 ttctttcgcc acaaccaccc gcttatattc ctctctcgcc aatgccaacg ccgcgcttat
      721 tttcgcggta ttggccggat tcaaaacgat tttatgaaat tcaggatcgt cgttttcaaa
      781 tttaaatgat ttgacgaaaa atttattgag ggcggtttta atcctatcca tcgaatcaac
      841 gggcgcgaat tctcccgtgc tcgccttgca gaaaagatcg taataggttt gtacttccat
      901 cgcgggcgag tgataactca ttatcgagtg ttcgatctct ctcgccttat caagattgat
      961 aacctttcca tcaactatta tttcatggga aatggtgcga ttttttatat ctatctgcgc
     1021 ttttaatttc tgcttctcgg caacggccat aaaaatggga gtgaattcgc cggacagcct
     1081 tgttttttct ctttgccgct taaagtgtat cgaatacaaa tttaaatcct tgtagatgtc
     1141 atccctacgc ttcgcctctt ggatggtaaa gtattcttta gctatctcat cgccaagctt
     1201 tatttcatta acgccgatat tcgtgaaaac ataggcctta ttcaattcag gatgatcctc
     1261 atagtgtcgg aattcgggca tacgcataat gcgcccgacg gtttggataa gaaacaccct
     1321 acttcccgtt tccctaaaaa taaccaatat ctgcgcacgc gggcaatccc aaccaagcgc
     1381 aatggcctgt ttgaaaatta aaacctcgac ctcatttgag ggcttttcaa tgttcaccaa
     1441 gttcggcgtt ttattttccg cgagccagat cgccagtcgc tcgttgtcct cggttatgtc
     1501 atgtttcgct ttcaagattt tctgcacatc aagtaaaaca tccttgtcta aatcgtttat
     1561 gcctttttgt aagtttggta attgaataag caaaagcgga ttcacattac ttccctcggc
     1621 tttatatgcc ttacaaagct cctcgcgttt cgctatcgcg gcattgataa ccatttctcg
     1681 actgctcttg ccgtccactc tgaatttttg gaattcggga ttgatggcaa tttctttttt
     1741 gatcattccc ccatcaatca ctttttcaaa atggactttt acatgagcgt cggggtcttt
     1801 aaatatcggc gtggctgaaa cttcaagcgt gatctttgcc gcaatgtcgg cgcgcaccct
     1861 tgtggcgttc ggccccaagc tcgtatgatg gctttcgtca ataatcagaa taatatccct
     1921 gcccgcttgc ttggtatttt cgacgatatt ggagagattt ttacctttct cgttatcacg
     1981 aatataaacc ctgtcttggt tataaatttt ttcccagttt aaaaacaaaa tctcgttctt
     2041 tccgatgaca ttgtttctca agtcctcgaa atgcgaataa cgaaagtcat tctcaaaatt
     2101 ctttgttaat gcttttttgc tttggttatg caaatcgcgg acggaaatcc aaatgaatga
     2161 aaactgcttg ctgtcttctc gatcaatcgg attcacaaga cgctttaaaa attcccccac
     2221 cataaccgtt ttgcccgatc ccgtaggggc ttcgaatata caaagtgaat tgccgtccag
     2281 ttccaaaagt tcgttgatct tgtccttcaa ctcctcaacc ttttcttcct gataatattt
     2341 taattgcaaa ttcggttcca tagttatttt t
//
LOCUS       DAIDDT010000073         2186 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_73_length_2186_cov_5.982456, whole genome shotgun sequence.
ACCESSION   DAIDDT010000073 DAIDDT010000000
VERSION     DAIDDT010000073.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73_length_2186_cov_5.982456"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          554 a          461 c          550 g          621 t
ORIGIN      
        1 ataaagaaaa tgtcaatttt ttttgacgcg agttcaagct tgaaatgcaa tatctctttt
       61 gggaacaccc tattcggcca cgataaaggg gggggcgcga acgatcctta tttttattgc
      121 gtgggtggat gtaaaagttt tattttccga aatcaagggc aaaaggtttt tgaggatgcg
      181 cgggcgttga ttgtaaatat tccaatagcc cgtcaatcgc agttttgacc tcatccggct
      241 tgattgccgc tacgcatggc gccggtatat ttctgtcggc aatgcactgg ccattgtcgc
      301 ccatgcaggg agcgtttttt tggaacaccc aacggttgat tccttcgtat ccatagaacg
      361 tccagagtcc cgggttccat gcgcccgaca ctaccatgca tggttttttc aaagcgatcg
      421 ccatatgccc taccgaagtt tcgttggaaa gcgtgaaaag acaatttttc atcaatgccg
      481 ctatgtccaa gaggccggtc atgccggtga ggtttgtgac ggggcgttga acgagccgcg
      541 ggaaggattt ttttctttcc gcatctcctt ttgttccgat gagtacgacg ggaatatttt
      601 tcgcggcgag ataatcgcat acttcggcgt atttttccat ctcccattct ttttccgccg
      661 ataaccctgc ggtgagcgaa agaaggcaat agccgttttc ccggatgccg tttttggcaa
      721 gcaggttttg cgcctccccg ggtaaacgat gcccttccat cacgagctcg gtggcgtatt
      781 cttgttgggg agcatgggca cgctcgctta catactcgaa tatcctgatg tggtgattga
      841 tgatatgggg aagatacttc ataccgtcgg aaattctcca ttgttcgatg gtattcgcaa
      901 taacgtcggt gtagaggggg aatatttttt tcttgatgta cgaatatccc cgcgcgtaac
      961 tcggcgtaat cggccgttct atatctatat ggaccccttc atatccaatg acccgttcgg
     1021 cgcccaattc tgcggcgatg agttttccgg cccattccaa gtggggatta tgctctacga
     1081 cggtggaaaa acctatgctt ctgagttttt tgacgaaacg gtagccatag cggaaatcgg
     1141 cgttaaattt ttttggattg ataagaatga tatggtcgat atatccccgg aatagcgcag
     1201 ggtcgaagcc attcgcgccg cttatcaata aagatatttc tttgtccgga aatgcgcgtt
     1261 tataatgttt gagtaccgac gtgaagacaa ccaggtcgcc gatggcgccg agattgatga
     1321 ggagcattct ttttgccaat ccgcttttat tggtccgcgc gaacaagagg ccgagcaaca
     1381 gaagacgcgt gcggcgaacg gcccgtttca ttatgtgttt caggttctct ttcatatttt
     1441 ataaggttac cattttgaac taatatatta tactggtatg atgaatgcaa tgagcggcct
     1501 taccatttcc ataataaccg ccgtctataa tggcgccgat tatatcgaag agaacatttt
     1561 aagcgtaaaa aatcaggatt atccgaatat cgagcacatc atcattgacg gaggatcaaa
     1621 agacgggacg gtggatatca ttaaaaaata tgccggaacc tatcatttaa aatgggtttc
     1681 cgaaaaagac ggcggaattt attatgcttt taataaaggg tttgccatgg cgacgggaga
     1741 ggtttatgca tggcttgacg gcgataacta ttttcatccc ggtatcgtaa agaaagttgc
     1801 cgatgttttc gaaggaaata atggcattga cattgtgcat ggcgatattg agattgtcgc
     1861 gaataatggg aggcataggg gcatctatcg tgcgccggaa gtttctttta aaaatgcgct
     1921 cctgaaaaat acgggggcga taccgttgca gccgtccgct tttttaaaaa aagaacttta
     1981 taaaaaaaca ggcgggttca ataccgacta taggattgcc gcggactatg atttttggct
     2041 gaaggtttta aaaaataatc cgcaaatata ttgcctgcgc gacgtcatcg gatcatatcg
     2101 aagaggcgac gaagccgctt cgcaatcatt gaagggggtt ttagcgggat atcgggaaat
     2161 gcttgccatt ggcgatgcct attgcc
//
LOCUS       DAIDDT010000074         2001 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_74_length_2001_cov_8.876819, whole genome shotgun sequence.
ACCESSION   DAIDDT010000074 DAIDDT010000000
VERSION     DAIDDT010000074.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74_length_2001_cov_8.876819"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          484 a          487 c          560 g          470 t
ORIGIN      
        1 atataatacc tatccataat atggtaccat atccgtttta ttttgataca atcatcggtt
       61 gtgcttaatt ttagggggat attcggcaat aggggcggcc tttagcgaca aaattgaaac
      121 tttttttccg gaaattcgta ttatataata atgcctggaa gctccttaaa agcagggtgt
      181 atggtttgtg tctgttgtcg ttcgcatctt tctgtccgtc atcccgcttt gtgtaggatg
      241 gcatagggcg ggaatccata ttttaaaaga ccggaagcat gagtttccgc tttcgcggga
      301 atggcggatt tttacgggat taacggaaaa aataatcaaa catcatagcc atgagcatct
      361 tcaacgatca ttataaaaaa aatcttgccg ccatcgttgc gatcgtcctc attattttcg
      421 ccgcagcggc gtttttgaat tccggttcgg catcggctgc cgcaatcgcc agtttctgga
      481 acggcagggt tctgccgttt gttttccgga aatacgaaca taatgccgcg atgcaatcat
      541 ccgcggcagc tggcaatacg tcgctttatc agccggcgat cgattacgag caggcggtcg
      601 tggatgccgt aaaaaaagcg tcgccggcag tggtttccat caccatttcc gagaacgtgc
      661 cgatcgtcgc acagtgtccg tataatccct atcaaaatct tccgccggaa ttccagcaat
      721 ttttcggcgg cgatgcatcg ggccttacgg ccccgtgcga taccggcaag acccagttgc
      781 aggaagtggg aggcggcagc ggttttatta tttcttcgga cgggctcatt ctcacgaaca
      841 agcacgtggt ttcggacgcg ggcgcgtcgt ataccgttct tatgaacgac ggtaagaaat
      901 attccgcgaa agttctggca agggatcctt cgcaggattt cgcggtggtg aagatcgatg
      961 cgaccggact gcccacggtg acgctcggtg attcaaacgg cctggcgctc gggcagaccg
     1021 ccatcgcaat cgggaatgcg ctcggcgaat acagcaatac ggtatcggtc ggcgtcatat
     1081 ccggcctttc gcgcaccgta acggcgggtg atccgaattc cgcgcaggag gaaacaatcc
     1141 gtggcgtgat ccagaccgat gccgcgatca atcccggcaa ttccggcggg ccgctgctga
     1201 acttgcgcgg cgaggtgatc ggcatcaata cggcaatcgc atcgggcgcg caaagcatcg
     1261 ggttcgcgat cccgatcgat caggcgaagc atgatatcac ttccgtggaa acgagcggcc
     1321 agatccagac gccctatctt ggcgtgcggt acatggcagt gacggcggat atggcgaagg
     1381 cccaaaatct gccggttgcg agcggcgctt tggtgcgcgg cggtgcgggc ggtccggccg
     1441 tgatgccggg atctcccgca gcaaaagccg gcatcaaggc ggaggacatc ataaccaaag
     1501 tggacaatca ggcgatcgac gccgatcacg atctcggaac ggtgatagcg caatacaacg
     1561 tgggtgatac ggtgacgctt acgataaacc gcgcagggaa agtcattacg ttgcaggcga
     1621 cgcttgcgaa aaggccggcg aatcagtaac ttcttgactc attggcactc tcggtatagg
     1681 attgccaatg agttattttg ccctctgtca tcctggaaaa cgcgagtatt cgggcgttta
     1741 tccaggatcc ggaattaatt ttctttggat gatgatgaag agggtccgga tcctggatat
     1801 tccttgcgga ctcggaattt caggatgaca caagggaaaa ccatcatcgg acgacaataa
     1861 tgataaaaaa ttgttatact ataactagaa cccgtctcga aaataggcca gcggggattg
     1921 cgatgaattt tcggagcgga aagcgcgcat tgcaacgaaa gaatatcggg atcaggatat
     1981 ttcaaggcga agtccgcgct t
//
LOCUS       DAIDDT010000075         1802 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_75_length_1802_cov_7.828986, whole genome shotgun sequence.
ACCESSION   DAIDDT010000075 DAIDDT010000000
VERSION     DAIDDT010000075.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75_length_1802_cov_7.828986"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          456 a          416 c          409 g          521 t
ORIGIN      
        1 tatacctata cggaagcggc gaaaaagata ctcgctgcgc gcggcgcgag cattgagtga
       61 cgtgcgtgca ataatgttgg gatgattttt aattataaaa tggcaaatca cataatcttt
      121 cttatgctgt tatcgtatac ctaatatcta tatatagaga gacataaagg tcgatagccc
      181 atataattat cattgttata aaaaagggcg cttgtaaggc gtcctttgtt gtatttaatt
      241 aagctgaccg tttcagaata tttggcggca acgatctttg ctgctccatg gaatactcgt
      301 agcgaacacg ggaactggtt ggattaaacg gtatgccgag tttttttcga atgtcacgct
      361 ccagattatt tcgaagggag acaatttctt ccggctgaag aaaatccgta gagacaaacg
      421 aagtatatcc tccgtcgggg tcgcccttgt agtattcggc cacggcgcaa tagtcaattt
      481 cttggctata aagcctgtcg ccgaaataag agtaaatcca agtaccttcg cggtcggggt
      541 gcggaattgc atcgtcgtaa tacggtgtcc ccggatagac ggtgatgatg gtcgcgtcga
      601 agtcatcagg attaaccgct aaaagccact cgtaggtttc gtggatggtt tcgcccgatt
      661 cacccggatg tcctatagac atcaatgctt ttaccttgag accatatttt ttagcaatct
      721 ccacgcaacg ggtattctcc tcgcggcttg cccgtttatt cgtgttgcgt aaaatgcgtg
      781 gagatccgga ttcgaagcca accaagatcc atctgaagcc ggctctccac atgctctctg
      841 cctgttcttc cgtgaatagc tgggatttga tgaagccacg taagcggaat tcaactccga
      901 gcttctcctg gagattggct atggcattca tcatatccac gactaatctc ttatttacgt
      961 tcaattcgtc gtcgtagaac atgaaacctg ttacaccgta cgtgctgtag aggtgactga
     1021 tttccgccac tacgttttgc gtggacctgg ttctaatttt tctcaggaac ggagaatttc
     1081 tacctccgca aaaaccacag ctgaatggac accccagttg tgaaatcaag cttagggcac
     1141 gatgtccttc aatggaataa ttgtagctac caacatcaat gaggtgtcgg gctggccagg
     1201 gtagaatatc gagatcggtg tttttcaaaa acaggtcggt tttgggattg tcggcgtcaa
     1261 tcagttttgg agcgttttcc tctattgcgt gaaagatggc tttttctcca tctcctgcta
     1321 tgaggacatc aaaccttttt tccagttccg cgaaagcaat tgtggcccga cctttcacac
     1381 cggttaatgt ttcctttcgg taggcggcat tgacaagagt ggtgtgcggc ccacctaaga
     1441 tcaccttgga ctcaggagat tctcttctta taacattcgt tattttgccg acagccggca
     1501 tttgaggcgt ggtagccgta attccgaatg tgcggattgg cgaataccgc agataattca
     1561 ctatcacatc ctcgtaattc tcgatgcccg atagatcaag tacttcaacc aaaaactttt
     1621 tttcaagtac tgcggcaatc ctgaggattc ccagggccat gaacacgcgc tcatcgagca
     1681 aaaacgctga cggcggtatc accaaacaga ctccagtatt ccgtttcatc gtctaacctc
     1741 cttttctaat attctgttat tttattattt agtttttgcg cccacgcgcc actgaataaa
     1801 aa
//
LOCUS       DAIDDT010000076         1684 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_76_length_1684_cov_6.123211, whole genome shotgun sequence.
ACCESSION   DAIDDT010000076 DAIDDT010000000
VERSION     DAIDDT010000076.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76_length_1684_cov_6.123211"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          431 a          378 c          473 g          402 t
ORIGIN      
        1 gatttatagt acgccgacat gtgtttactg caggatggcg aaggattttt tcgtgaagaa
       61 taatgtcgcg tatgtcgatc atgatgtcgc ggcggatgaa gcggcgcgga acgagatgtt
      121 ttcgaaatcg catcagatgg gcgttcccgt gattgacatc gacgggacga ttatcgtcgg
      181 gttcgataaa gggacgctgg aaaaagcgct gggaatcaaa taagcggtga gttatattgt
      241 tttaatgttg cagatgttct catggaaagg aaggttataa catcataaca ttacaacctt
      301 gcgacactcg aacattacaa cgatttatca tgtacgatct tattatcatt ggcgcgggac
      361 cgggcggtat tgccgcgggc gtgtatgccg cgcggaaaaa aatgaagacg cttttgatca
      421 cggacggctt cggcggccag tcggtggttt cgaatgatat cgggaactgg atcggggagc
      481 cgtccatctc cgggtacgac ctttcgaaaa aactcgaaat gcatctccgc gtgcaggaag
      541 atatcgagat cgtcgacgat gacttcgttg taacggcgaa aaagatcgat gccggatttt
      601 caatcacgac gaagagcgga aaaacactcg aaacgaaata catgcttctc gcgtcgggaa
      661 gccaccggaa aaaactcggt attccgggcg agaaggaact agacggcaaa ggcgtcgttt
      721 attgctcgac ctgcgatgcc ccgattttca aaaacaagac cgcggtggtc gtgggcggcg
      781 gcaattccgc gcttgaggcc gtggtggatc tttttccgta cgcgtcgaaa atttatctca
      841 tggtgcgctc ggaagttttg aagggcgatc tcgtgacgca ggataaaatc aagacgcatc
      901 ctggcgtgga aattctttgg aatgccgttc cgcaggaggt cttgggaaag gattctgtga
      961 cgggcatccg ctatcgggac gcgaagagcg gcgaggtgaa agacctggcg ctggacggcg
     1021 tgttcgtgga aattggtctt ttcccgaact ccgatttcgt gaaagatctc gtgaagctgg
     1081 atgattacgg agcggtcatc gccgatccga aaacgcagca aacgtcctgc gtcggcatat
     1141 gggcggtggg cgacgtgacg gacgcgctct ataaacagaa caacattgct gccggtgatg
     1201 ccgtgaaagc agtgctgaat atttacgatc agctgaagaa gcagtagttt taaaacgcac
     1261 ctgaaactct ccgtcgttct taccctgtat ggtgataggg gataaactcc gccgaaaatc
     1321 cattcttttt ttctccgtca tcctcgactc cggtcgagga tgtatgcttt aaaaaattaa
     1381 aagataagat gggaagcatg ggtagtccgt ccgtcttcac ggatgacgac agaggaagcc
     1441 gaacgatgac aaatttgcga tctggctgtt ctgtccgcaa cggagctttc ttattcgaga
     1501 aggagttaga ggagcccgtc cttttccgca ccagacgaaa catctctgtt tgtagcgggt
     1561 ttaagaaggc ctgccatttc tttccgcacc agacggagct ttcctgcttt caaaggacta
     1621 aaaagagccc ctccttttcc gcaccagacg gagctttcct gctttcaaag gactaaaaag
     1681 agcc
//
LOCUS       DAIDDT010000077          610 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_77_length_610_cov_1.345216, whole genome shotgun sequence.
ACCESSION   DAIDDT010000077 DAIDDT010000000
VERSION     DAIDDT010000077.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77_length_610_cov_1.345216"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    310..319
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          147 a          161 c          160 g          132 t
ORIGIN      
        1 accgggaatc ggacctggat ctctcatcac ctcctcttaa acaacataag gcaagaacaa
       61 aaccactcca gcgagagctg ccttgaactc gagctggagt ggttttgttc tcgcctgtgc
      121 cctcgagagg aatcgaacct ctattacaag atccgcaatc ttgcgtccta tccgttgaac
      181 gacgagggcg tcgtcgtttc tgcggccgcg gttgtgcgca gcgcacctat agcgtactag
      241 aaccggagga gtttttcaat ggaatcaatg agcgtgggct cgtaaggaac ttcgcggagg
      301 atggtcttcn nnnnnnnnnt gcggagttcg gtcgcgcgcg cacccggcat aaggattttg
      361 aacggcgtct tgagcttggc gcggaacgtg aagatgatct ccgcaagatc gtttcccagg
      421 tcttcaacgc tgaagctctc aaactcgcgg agcggatggg acttaccgtc cgttacggct
      481 acttcgttct ccgtgagcgt gaatgccacg agccgcggcg aacggttgcc ccatacaata
      541 aagagtatct ccgcgatcac aataaaaaat ccaaacagaa agttccgttc ccaaacggag
      601 aaagcaatta
//
LOCUS       DAIDDT010000078          595 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_78_length_595_cov_1.471042, whole genome shotgun sequence.
ACCESSION   DAIDDT010000078 DAIDDT010000000
VERSION     DAIDDT010000078.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78_length_595_cov_1.471042"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          141 a          157 c          164 g          133 t
ORIGIN      
        1 ggccggaagt catcatccag tttgaccatc acttccgcac gcccctgcgc atcaaggcgc
       61 cgatgaactg gctcccggaa atacggcggg agttccgggt gcacatcccg gaggagcatt
      121 ttgaaccggg cctggcggac gtgctccaaa aatatctgcg cttttaaatc ggcgcgggtt
      181 cgggtaatat ggggaacgcc cgccctcgtc gttcaacgga taggacgcaa gattgcggat
      241 cttgtaatcg aggttcgatt cctcgcgggg gcacgggcat gtgagaccga ccgaggtgcg
      301 taaatgctac gtttttttga ggcggtcaag gcgtacccac ccaggaatcg aaccggcgct
      361 ctgcgtatat caatattaaa gggtttttaa aattggcttt ttcgccgatg cgataatttg
      421 tttcaaagac tcgaactagg cattatataa tgcagacatg agcaaaaccc agattcgcct
      481 tgtcggggtt ccggtgcaaa ccaaacccac catcttcaac gccgtccgga aggccattgc
      541 aacggggcgc aggcagcttg catatcaagt acccatcacg gtcgtaatat tcgag
//
LOCUS       DAIDDT010000079          595 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_79_length_595_cov_0.870656, whole genome shotgun sequence.
ACCESSION   DAIDDT010000079 DAIDDT010000000
VERSION     DAIDDT010000079.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79_length_595_cov_0.870656"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          148 a          152 c          167 g          128 t
ORIGIN      
        1 actataacca gcgtgatgcg gcgtggggaa gtgatgcact cgacgggacg aaatacagct
       61 tggcaagcgc cggttgcctg atcgcgtccg tggcaatggt gcttacccat tacggctatc
      121 gcgacgtgac acccgcgacc atcaatgcgg atccggataa ttttgcggcg tactctcccg
      181 acctgctact cgcgacgatc agcgtagacg gggtgaccgt cacgcggaag gccgtgaatg
      241 tgcgcggtga caccgccgca cgcaagatcg ctgttattga cgcgatgctc gcgaccggcc
      301 atccggcgat cgtcggcatg aatgccctcg gcggaacgca ctttgttgtg ctgacaagcg
      361 gaagcaaagg cgattacctt atgcaagacc cctatgtggc gaacggcgac aatattggtt
      421 tttcaaccca ttattcactc aaagagattt acagtgtcgc aagggttgta atcagtagtt
      481 agcgctgttc acgggggatt tgacaagaaa taccctaggg gggtataata gcactattca
      541 gcgcaaaagg agccatatcc ctaaaaatgc tgctagcaaa tcattttaat aaagg
//
LOCUS       DAIDDT010000080          593 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_80_length_593_cov_1.676357, whole genome shotgun sequence.
ACCESSION   DAIDDT010000080 DAIDDT010000000
VERSION     DAIDDT010000080.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80_length_593_cov_1.676357"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          163 a          140 c          163 g          127 t
ORIGIN      
        1 ataaagcgtc gctgcttaac gataaaggtc cgatcgatgc gtcatgcgag tgcctggtct
       61 gccggaacta tcggcgcaat tacatcgcgc atctcgtgcg cgcgaacgag atcacgggcg
      121 gcgcgctgct cacgttccat aatttgtttt tcttcaatac gtacgtggaa aaagcgcggg
      181 aaaagatagc aagtggagcg ttgtaatttt cgacggaagt gtacattccc ggcgcgttag
      241 tacgcttttg aacaaatgaa agactcgggt gagaattgag actatgggag tccacccaaa
      301 ggtcgcaaat caacaaaccc gagtctttca tgcataaaaa taccaaactc ttgccgcgga
      361 tgcgacagga gctctaccgg agatggatcg acggcgatac catcgcggaa cttgctcgcc
      421 attacgggat ttcccgggcg actgtgtatc ggacggtaaa gaaggcgcgt atggatatct
      481 tcgtgaatct cccgtcaatg aaccaccggt atcgcaagat ccagtatggg ttgaagaagc
      541 tcacgaagac agaaaagata ttggcaaaga agcttgcgcg gagagcacac cgt
//
LOCUS       DAIDDT010000081          579 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_81_length_579_cov_0.786853, whole genome shotgun sequence.
ACCESSION   DAIDDT010000081 DAIDDT010000000
VERSION     DAIDDT010000081.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81_length_579_cov_0.786853"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          138 a          170 c          156 g          115 t
ORIGIN      
        1 agcttgcgcg gagagcacag cgtctcaccc gttacgagaa ggccactccc ggggaaatga
       61 tccactttgg cacgaagaaa ctgcccgtca tgtacggaga gtccgtccgc gcccctcgcg
      121 aatggctcca tgtggcaatc gatgactttt cgcgggatct cgttgcggat attttgcccg
      181 acaagacggg atattcttct gccatcttcc tcgaagaagt catccatgca atgccgttcc
      241 gcatcgaggt ggcatattcc gacaacggcg gcgaatatcg cggacggcct gatcatccct
      301 tcgtcgcggg attggcccgc cacaagatca aacagcagca tacgaagccc cggcatccgc
      361 agacagatgg caaggcggag cgggtcatca agaccatcat gacggaatgt ttctcaaggg
      421 gagggagaac cttctcttcc cgtgatcaga ggcggaaatt cctctacgca ttcgtgaact
      481 ggtacaatca agtgagaccc catgagtccc tgggcggcat atcgccgctc caaaggatcg
      541 aagcctatct tgcccgagta cctactgccg aaaatgaac
//
LOCUS       DAIDDT010000082          572 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_82_length_572_cov_0.905051, whole genome shotgun sequence.
ACCESSION   DAIDDT010000082 DAIDDT010000000
VERSION     DAIDDT010000082.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82_length_572_cov_0.905051"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          121 a          139 c          209 g          103 t
ORIGIN      
        1 ctgcgattcc gctccggtgg tgatctggaa cgtcttgccg ttcgtcgtat aggtatagta
       61 gaggcaggag gacaccttgt tcacgggatc cacgggaagc ttggggagcg atgcccccat
      121 gccggtgccc gtgaggttca cggggatcca tcccgtgccg ttggtgtccg tataggtggc
      181 gggaggggcg caggcatagc tccagccgtt cgggagcgca ggaaggccga ggcctccgca
      241 ggtggaggaa ctgtcgggga tcgagacgta caccgtgttc gcggtgccga gcgagaggcc
      301 gttgaacgac gcgagggata gcgcgttcgt cagcgtgttc atgtcggaga cccggttcgc
      361 atccctcgat tgggaaagca gctgtgcggg attcaacgta aggaccacga ccaccgcaag
      421 gatggcgatg atcgcgatca cgacgagaag ctcgatgagg gtgaaggcgg agcgggatct
      481 gcgggataaa gaggcacggg gagacatgag aaaattatac catgacagga tgcatgggaa
      541 agacgatgcg tctcgatgct attgccgctc ag
//
LOCUS       DAIDDT010000083          571 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_83_length_571_cov_0.825911, whole genome shotgun sequence.
ACCESSION   DAIDDT010000083 DAIDDT010000000
VERSION     DAIDDT010000083.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83_length_571_cov_0.825911"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    236..249
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT           98 a          152 c          150 g          157 t
ORIGIN      
        1 tccctacctt gcgtgggtgt tctttgccgt gtatctcaac cttggtattt ggatgctgaa
       61 ctgattggac gcatacaagc tgttgatata tcctgtctcc tgtttacggg cctatagtaa
      121 agtggtatta cgcggcattc gcattgccga gtccggggtt cgactccccg taggtccacc
      181 gccatgacaa agctcggcga gattgcactc aacgtaacgc tcttggcgcc ccgggnnnnn
      241 nnnnnnnnnt gtagtctcac tgggcacgct cgcggattgc gtgcgtaact tccaccgtcg
      301 cacacaggta ttgccggcgc tgttttgatt aatagccgtg gtccttttcc atctcgcttg
      361 catacgttcc ctgcgctgcc agcgagttca ttttggagcg gaatgcgagt gcgctctggg
      421 ctcttgctac gttcctcttg atgtcctgcg accaaatttt gaggacgggg ttttggatag
      481 cacgcgagta ggaaaaagaa agcgcccatg gcgtaccgcc aatcttgttc atcgcgttga
      541 gattttcagt cgcctgctca tcgccttggc c
//
LOCUS       DAIDDT010000084          552 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_84_length_552_cov_0.976842, whole genome shotgun sequence.
ACCESSION   DAIDDT010000084 DAIDDT010000000
VERSION     DAIDDT010000084.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84_length_552_cov_0.976842"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          140 a          130 c          117 g          165 t
ORIGIN      
        1 ttccgattat acgagctttc tttattccgt ttcttactcc aaaataaaaa acggaatatt
       61 agtcaggaaa acagagatgg atccggcagt aataataacc accgtcgtgc ttctcgtaat
      121 ttttatatcc ttccttttgt cctttttaac cgtgattcga cggaaaagga taccgtgcta
      181 cagcggcaat gtaaaaaaat accgcttcgc atggtaatgg tattgttctc tatggcctcc
      241 ggcgcgattt ttttcgcaac gccggaggct ttttttatct cttattatca agaatttgat
      301 cttcgtgtcc ggaaatagac tatctttaac caaccggttt tgcaatgatg tgcccggggt
      361 gggattcgga cccacaaacc cttacgggtc aaggatttta agtcctccgc gtaaaccgtt
      421 ccgccacccg ggctcgcata tttatatatc gaccaatttt aaatacaggc tgatataaca
      481 agacctggag gcgtgggagg gattcgaacc ctcgctgcgc ttttggcgca gacagtcttt
      541 ccaggactgc ac
//
LOCUS       DAIDDT010000085          541 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_85_length_541_cov_2.956897, whole genome shotgun sequence.
ACCESSION   DAIDDT010000085 DAIDDT010000000
VERSION     DAIDDT010000085.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85_length_541_cov_2.956897"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT           91 a          132 c          156 g          162 t
ORIGIN      
        1 cccggcgata cattcgcgct tgagtttgcg cagatgaaga ttttcaatta cggtctgctt
       61 tttgcgctcg cgcttatgac cggcgcgctt cttgcgcttg aatttttcgc attcaggcgt
      121 tctcgcatac agggcttccg cgccgtgagc aagagcggtg ttggcgttgc cgcttcgctt
      181 gccggaggaa tacttacggt tgcttcgtgc ggttgcggta ccggcatttt gcttggcgtg
      241 ctcggacttg gcggaggtgc gtttttcgtt gcggcgaacc agacggccat cgccgccgtc
      301 atgctcgccg tcgttgcggt cggtatctat ttttcggcgc gccgcgctgc gggaatttgc
      361 gcgacatgcc gtgttgagtg atctttgcgg tttgagatat tttcttatga tttttttaat
      421 gcgtaagtcc taaaaaacac acgcagaatc ggcataaaaa aggatcgaat gttcatctct
      481 gtttattccc gtagcagtgc tgtcgcggcg cttgacaatg actgcatcgg gagtatatac
      541 t
//
LOCUS       DAIDDT010000086          538 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_86_length_538_cov_0.917570, whole genome shotgun sequence.
ACCESSION   DAIDDT010000086 DAIDDT010000000
VERSION     DAIDDT010000086.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86_length_538_cov_0.917570"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          147 a          146 c          109 g          136 t
ORIGIN      
        1 acctaatagt gacacagggt ctccatatta tgtcctccga ctctacaatt cggagttccg
       61 ccaaaacagc agatgatttt aaataacgct tcccggtgat accgctgccc aaaagacagc
      121 ttacgtaaca ccgaaagata caaagccaag aaaaaaaatc agccgctcag cggccgctcc
      181 atcgcgtaga atacgcgcgg tcagccgtct tgctgtgaaa gattagtgtt ctttaccatt
      241 cgtatcttag gagtaagtat actcaggtca aaaaagatgt caatagctgc attgttgtcg
      301 ggccgccggg aatcgaaccc ggtctttacg aacccgaatc gcacgtacta ccggtatact
      361 acggcccgta gaataccaag gtatttaacc gcgtttagga aaataattca actaagcccc
      421 cgtcgtgccg aaccctccgc gcgttttatt tttcatctca tccacttcga tccattcggg
      481 atgggaaatt tttacgaatg tcccctgcgc aatgcgctcg cctttttcta tttcaacc
//
LOCUS       DAIDDT010000087          515 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_87_length_515_cov_1.152968, whole genome shotgun sequence.
ACCESSION   DAIDDT010000087 DAIDDT010000000
VERSION     DAIDDT010000087.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87_length_515_cov_1.152968"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    240..265
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT           88 a          160 c          159 g           82 t
ORIGIN      
        1 agccgatgat ctcgacgcag tggtttctga acatgaaggg catggccgac agggccctcg
       61 aggccgtcca ctcgggcaag accgagatca tccccgccga gtgggtgaag acctacgacc
      121 acttcctgga gaacatccag gactggtgcg tctcgcgcca gctctggtgg ggacaccaga
      181 tcccggcgtg gcatcacccc gacgggcgcg tcgtggtggc gcgcgagcgt ccgaaggagn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnacgtg ctcgacacct ggttcagctc ggcgctctgg
      301 ccgttctcga cgcaggggtg gcccgacacc acccacgcgc tcgagaagtt ctacccggcc
      361 agcgatctcg agacgggcta cgacatcctc ttcttctggg tcgcccggat gatcatgttc
      421 ggcgtccact tcatgaagcg cccccccttc acgcgcgtgc tcctccacgg gctcgtcgtg
      481 gacgagacgg gggacaagat gagcaaggtg aaggg
//
LOCUS       DAIDDT010000088          510 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_88_length_510_cov_1.593533, whole genome shotgun sequence.
ACCESSION   DAIDDT010000088 DAIDDT010000000
VERSION     DAIDDT010000088.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88_length_510_cov_1.593533"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          115 a          150 c          110 g          135 t
ORIGIN      
        1 tacaccccta gttgcctaag agctagcaga catagtttac ggctaggact acacaggtat
       61 ctaatctgtt tcgctgccct agctttcgtg cctcagcgtc aggaaaaacc caggtgcctg
      121 ccttcgcctt tggtgttcca gctaatatca acgcatttca ctgctacact aactgttcca
      181 gcatccctgc caatcctcaa gaatgtgagt atcctaacag gtcctgtggt tgagccacag
      241 tatttaagct aggactttac actccgccta cgcacacttt acgcccaata aatccggata
      301 acgcttgcct cctacgtatt accgagactt ctggcacgta gttagtagag gcttattcgc
      361 caaactgtga aagttttggc aaaaggagtt tacgacccta aggccttcat cctccacgcg
      421 gcgtcgctgc gtcaggcttt cgcccattgc gcaagattcc ccactgctgc ctcccgtagg
      481 agtctggacc gtgtctcagt tccagtgtgg
//
LOCUS       DAIDDT010000089          507 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_89_length_507_cov_3.820930, whole genome shotgun sequence.
ACCESSION   DAIDDT010000089 DAIDDT010000000
VERSION     DAIDDT010000089.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89_length_507_cov_3.820930"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          125 a          112 c          175 g           95 t
ORIGIN      
        1 ggtgggggac aaccgcggga aactgcggct aataccgcat gacgactgag gtccaaagct
       61 cgcgagagcg cctggggagg agcctgcgtc cgattagcta gttggtaggg taacggccca
      121 ccaaggcgac gatgggtagc tggtctgaga ggacgaccag ccacactggg actgagacac
      181 ggcccagact cctacgggag gcagcagtgg ggaattttgg acaatggggg aaaccctgat
      241 ccagccatgc cgcgtgagtg aagaaggcct tcgggttgta aagctctttc gtgagggaag
      301 aaaggacttg agtgaatagc ttgagttgat gacggtacct caagaagaag caccggctaa
      361 ctacgtgcca gcagccgcgg taatacgtag ggtgcgagcg ttaatcggaa ttactgggcg
      421 taaagcgtgc gcaggtggtt ttgtaagtca gatgtgaaat ccccgggctt aacctgggaa
      481 ctgcgtttgt gactgcaagg ctagagt
//
LOCUS       DAIDDT010000090          506 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: bacterium isolate clean5442
            NODE_90_length_506_cov_1.930070, whole genome shotgun sequence.
ACCESSION   DAIDDT010000090 DAIDDT010000000
VERSION     DAIDDT010000090.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081276
            Sequence Read Archive: SRR9320197
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (mine drainage metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90_length_506_cov_1.930070"
                     /isolate="clean5442"
                     /isolation_source="water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA: Vershire, Ely Copper Mine"
                     /lat_lon="43.91924 N 72.28644 W"
                     /collection_date="2017-07-28"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          124 a          135 c          150 g           97 t
ORIGIN      
        1 gggtagtacg gccgcaaggc taaaactcaa aggaatagac gggggcttgc acaagcggtg
       61 gagcgtgtgg ctcaattcgt cgctaaacga agaaccttac cagggctaga aatcctactg
      121 cacgcctagc gaaagctagg aagccttcga gggtgtagga caggtgatgc atggctgtcg
      181 tcagttcgtg gtttgaatcg ttcccttcag tggggtaacg aacgcaaccc tcgttgcctg
      241 ttacaagtgt caggcgagac tgccccctca cgggggagga aggtgaggat gacgccaagt
      301 cagcatgtcc ctctgatgtc ctgggctgca cacgcgctac aatcgccttt acaaagggac
      361 gcaatgccgc gaggcggagc aaatccccaa aagaggcgcc agttcggatt ggagtctgca
      421 acccgactcc atgaagccgg aatcgctagt aatcgtgggt cagccacacc acggtgaata
      481 cgttctcaag ccttgtactc accgcc
//
